LOCUS       DAXWIQ010000001       102043 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000001 DAXWIQ010000000
VERSION     DAXWIQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 102043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 102043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..102043
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.001"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(444..1562)
                     /locus_tag="ACGEUI_000001"
                     /pseudo
     CDS             complement(444..1562)
                     /locus_tag="ACGEUI_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1867..2664
                     /locus_tag="ACGEUI_000002"
     CDS             1867..2664
                     /locus_tag="ACGEUI_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8164885.1"
                     /translation="MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSG
                     RSLLAIDQLFEPFLDQIAVIAENGSVVQYRGEILFADMMTKEQYTEVAKKILANPHYV
                     ETGMVFSGQKAAYILKGASEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHT
                     VLEGSDWLNQALPYATAVTTGFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDN
                     FNDYQMLEFAGRAIATENARPEIKVISDQVIGHCNDGAVLTYLKGLV"
     gene            2668..3492
                     /locus_tag="ACGEUI_000003"
     CDS             2668..3492
                     /locus_tag="ACGEUI_000003"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8164886.1"
                     /translation="MNIRILALDLDGTLYNTEKIVTDANKKALAAAREKGVKVVITTG
                     RPLKAIGNLLEELDLLDHDDYSITFNGGLVQRNTGEVLDKSSLSFDQVCQIQQALEAV
                     GLPTDIISGGDVYSIPSKDGRHSQYHLANPLLTFIEVTSVAELPKDITYNKIVTVTDP
                     DFLDQQIIKLSPSLFEDFEAFKSRDIIFEIMPKGIDKAFGLNLLCQHLGLDARHVMAM
                     GDEANDFAMLEWAGLGVAMANGVSGAKADADAVTTLTNDESGVAEAVKTFILEEES"
     gene            3492..5042
                     /gene="ftsY"
                     /locus_tag="ACGEUI_000004"
     CDS             3492..5042
                     /gene="ftsY"
                     /locus_tag="ACGEUI_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFF8164887.1"
                     /translation="MGLFDRLFGKKETPKVAEEKLEENLLTETTQKEELSEKANEQDK
                     IEAVQQEDVSSEGAGSVENGPEAASVNALVEEETGDNSNHPSEDTNEFAADKTDLKVS
                     ELSQSTASEPKDLVDQPVVEQFPTKQAQADASNDSANEEAVDTSKEQSSSQQVMEDYY
                     RRKAALEKSLQEKAAATVPVMPEEVPQENQASTSAEASQNKATHDTIPETDQEKYKRS
                     LKKTRTGFSARLNSFFANFRRVDEEFFEDLEEMLILSDVGVHVATTLTEELRYEAKLE
                     NAKKPDALKRVIVEKLVDIYEKDGRYNEAINYQDGLTVMLFVGVNGVGKTTSIGKLAY
                     RYKQEGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVITGPEKADPASVVFDGMEKAVA
                     KGVDILLIDTAGRLQNKENLMAELEKMGRIIKRVLPDAPHETLLALDASTGQNALSQA
                     KEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVKFIGFGEKVDDIGEFHSEDFM
                     KGLLEGIL"
     gene            complement(5096..6463)
                     /locus_tag="ACGEUI_000005"
     CDS             complement(5096..6463)
                     /locus_tag="ACGEUI_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8164888.1"
                     /translation="MSHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVY
                     QLPLNSLQWMTTIYLLAVAIMMTTSATLKKNVRERPLFFMATGLFTFGTILAVLTQSF
                     AIMLLARIFQGIGTGLVMPQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGF
                     MISHFSWQWIFICILPVPLIAGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLA
                     ITSLENGSVNLYYLGLFILSFILFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQL
                     INLGINFLTPNFIVMEKIANSSQAGMVLLPGTLLGALLAPAFGKLYDQKGARLSLYLG
                     NALFSLSLIIMTLQTRHFMLLPFTLLYILFTFGRNMGFNNSLATAIRELPAEKNADAT
                     AIFQMMQQFAGALGTAMASLIANSQAEFTSGVQSVYLLFTIFALLDFIFFFAMFYHLG
                     KKGLA"
     gene            6791..7633
                     /gene="licT"
                     /locus_tag="ACGEUI_000006"
     CDS             6791..7633
                     /gene="licT"
                     /locus_tag="ACGEUI_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFF8164889.1"
                     /translation="MLIKRVLNHNAAISTNHQGLDILLMGKGIAFGKKVGDSIELNAI
                     ETSFVLKNSDNMNRFTELFITVPQEVVACSERIINLGKIKLGKTLDEILYINLTDHIH
                     SAIERHEQGMLIHNPLRWEIQRYYPDEYSLGVKALELIERNLGVTLAIDEAAFIAMHF
                     VNASLDTPFKEPHRLTEIVSYIEQKIKTDFKTELDDTSIDYYRFMTHIKLFAQRVLSQ
                     MSYDDDDAELLLVVKTKYPKEYRCVLDISEEIKKRYNYHLNSSELLYLTVHVKRLVKH
                     LKET"
     gene            7635..9497
                     /locus_tag="ACGEUI_000007"
     CDS             7635..9497
                     /locus_tag="ACGEUI_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HFF8164890.1"
                     /translation="MTYQETAKAILAAVGGKTNIQRVTHCVTRLRLVLKNDEKVKDQQ
                     VKAISNVIGVMRKNGQYQIILGNDVNNYYQAFLSLGHFDNQDEDHSSKAKGSILERLI
                     ETIAGVITPLIPALLGGGMLKVVGILLPMLGLASADSQTVAFINFFGDAAYYFMPVMI
                     AYSAAARFKVTPVLAATIAGILLHPAFVAMVAEGKPLTLFGAPVTPASYGSSVIPILM
                     MVYLMQYIEKWVNRLVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGQGLSNTMLA
                     IYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVATPDVLILPAMLASNLAQGA
                     ASLAVAFKTKQKQTRQVALAAGISALLAGITEPALYGVTLKFKKPLYAAMISGGLVGA
                     FIGFVNIASYTFVVPSIIGLPQYINPSGGANFTNALIAGTATIVLAFSLTWFMGIDEE
                     SPKQVSVAADMSQVKSGLSTKQTLYAPMTGEMLFLSEVPDETFSSKLLGEGFAILPSE
                     GEVYAPFDGEVITFFPTKHAVALKNTRGVEVLIHVGIDTVELKGQGFEQLVSVGDVVK
                     RGQALLKMDIDFITSKGYSLISPVVVTNSAEQLEIIIQDDKKMVTKEDALLVIL"
     gene            9516..10940
                     /locus_tag="ACGEUI_000008"
     CDS             9516..10940
                     /locus_tag="ACGEUI_000008"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8164891.1"
                     /translation="MGIFPKDFLWGGAVAANQVEGAFEADAKGLSVQDVLPNGGLGEW
                     TDSPTSDNLTLEAIDFYHRYKEDIALFAEMGFKVFRTSIAWSRIFPNGDDDQPNEAGL
                     QFYDDLFDELLNYGIEPLVTLSHYETPLHLAKAYNGWTDRRLIGFFERFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGGIMTEKEKLSLQDLYQAIHHELVASASVTKLAHEI
                     NPDVKVGCMILAMPAYPMTSDPRDILAAHAFENLNLLFSDIHVRGKYPSYIKSYFKEN
                     GIEIVFEDGDKELLAEHTVDFLSFSYYMSVTQAHNPEAYTSGQGNILGGLSNPYLESS
                     EWGWQIDPIGLRLVLNQYYDRYQIPLFIVENGLGAKDQLVQTADGSMTVHDDYRIDYM
                     SQHLVQVAKAIEDGVEVMGYTSWGCIDCVSMSTAQLSKRYGFIYVDRNDDGTGQLTRY
                     KKKSFDWYRQVIQTNGRYLEDNFS"
     gene            complement(11039..11854)
                     /locus_tag="ACGEUI_000009"
     CDS             complement(11039..11854)
                     /locus_tag="ACGEUI_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFF8164892.1"
                     /translation="MIRFLILAGYFELTMYLQLSGKLDQYINVRYSYLAYISMILSFI
                     LALVQLYTWMKNIKVHSHLTGKIARLTSPFILVFPVLIGLLVPTVTLDSTTVSAKGYT
                     FPLAAGASKTGVSDDGTTIQYLKPDTSLYFTKSAYQKEMRQELHKYKGKKPVTITTEN
                     YMEVMELIYLYPDEFLDRDIQYTGFVYNEPGHDNYQFLFRFGIIHCIADSGVYGLLTT
                     GNQTSYPNNTWLTVKGRLHMEYDKNLEQHLPVLQLAEVHQTKEPNNPYVYRVF"
     gene            complement(11854..12756)
                     /locus_tag="ACGEUI_000010"
     CDS             complement(11854..12756)
                     /locus_tag="ACGEUI_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFF8164893.1"
                     /translation="MSLFSNLPPSVLQWFAIFMSIIIEALPFVLLGTILSGCIEVFVT
                     PELVQKYLPKQKCLRILFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSLRFLILRLVGAALVAITLGVMLAFIVDDNILKDNAQPVHFH
                     DYSHESLPKRIYLALVHAIDEFFDTGRYLVFGTLIASAMQIYVPTRVLTTIGHNPLTA
                     ILIMMLMAFILSLCSEADAFIGASLLSTFGVAPVLAFLLIGPMVDIKNLMMMVKAFKG
                     RFIVQFIGVSVLMIAVYCLLVGVL"
     gene            complement(12904..13059)
                     /locus_tag="ACGEUI_000011"
     CDS             complement(12904..13059)
                     /locus_tag="ACGEUI_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFF8164894.1"
                     /translation="MAITHKKNDELEKMLAGFASIPSFDKPLEVNTDGKLATKEKTTE
                     QTKPSKP"
     gene            13293..15425
                     /locus_tag="ACGEUI_000012"
     CDS             13293..15425
                     /locus_tag="ACGEUI_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFF8164895.1"
                     /translation="MENNNNHNIAEALSVSLHQIEQVLALTAQGNTIPFIARYRKEVT
                     GNLDEVVIKSIIDMDKSLTTLNERKATILAKIEEQGKLTDQLRTSIEATEKLADLEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNAQNLETAAEPFVTEGFASPQEALAGAVD
                     ILVEAMSEDAKLRSWTYNEIWQYSRLVSTLKDEQLDEKKVFQIYYDFSDQVSNMQGYR
                     TLALNRGEKLGILKVSFEHNLEKMQRFFSVRFKETNPYIEEVINQTIKKKIVPAMERR
                     VRSELSDAAEDGAIHLFSENLRHLLLVSPLKGKMVLGFDPAFRTGAKLAIVDQTGKLL
                     TTQVIYPVAPASQTKIQAAKETLTQLIETYQIDIIAIGNGTASRESEAFVADVLKDFP
                     NTSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLSENLGFVVDTVVNQVGVNVNTASPSLLAHVSGLNKTISENIVKYR
                     EENGALTSRADIKKVPRLGAKAFEQAAGFLRIPGAKNILDNTGVHPESYPAVKELFKV
                     LGIQDLDDAAKATLAAVQVPQMAETLAIGQETLKDIIADLLKPGRDLRDDFEAPILRQ
                     DILDLKDLEIGQKLEGTVRNVVDFGAFVDIGVHEDGLIHISEMSKTFVNHPSQVVSVG
                     DLVTVWVSKIDLDRHKVNLSLLPPRDTH"
     gene            15412..15849
                     /locus_tag="ACGEUI_000013"
     CDS             15412..15849
                     /locus_tag="ACGEUI_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019318761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFF8164896.1"
                     /translation="MTLTNYVQEVSLADFGKSLHHKAYWNKRLKTTGGRFFPKDGHLD
                     FNPRMLEEHGELIFRKIVRHELCHYHLYFEGRGYHHKDRDFKDLLAQVNGLRYVPTSS
                     KSKTNHHYSCQTCGQVYQRKRRINLAKYVCGNCHGKLMEKNQS"
     gene            15913..16185
                     /locus_tag="ACGEUI_000014"
     CDS             15913..16185
                     /locus_tag="ACGEUI_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFF8164897.1"
                     /translation="METKFYKQRKNRLVAGVIAGLADKYGWDLALARVLAALLIYGTG
                     FGVLLYILLAIFLPYKEDLLEERYGRGPRRRKDADVLNEEEDGWFW"
     gene            16550..17482
                     /gene="hprK"
                     /locus_tag="ACGEUI_000015"
     CDS             16550..17482
                     /gene="hprK"
                     /locus_tag="ACGEUI_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFF8164898.1"
                     /translation="MTVTVKMLVQKVKLDVVYATDNLLSKEITTSDISRPGLEMTGYF
                     DYYAPERLQLFGMKEWSYLTQMTSHNRYSVLKEMFKKDTPAVVVSRNLAIPKEMVQAA
                     KEEGISLLSSRVSTSRLAGEMSYFLDASLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAIYLENFEAGKVFDRLGNGNEEITFSGVRIPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKEMGFDATKTFEDRLTQLITKNEVSQ"
     gene            17479..18258
                     /gene="lgt"
                     /locus_tag="ACGEUI_000016"
     CDS             17479..18258
                     /gene="lgt"
                     /locus_tag="ACGEUI_000016"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFF8164899.1"
                     /translation="MINPIALKCGPLAIHWYALCILSGLVLAVYLASKEAPKKGISSD
                     AIFDFILIAFPLAIVGARIYYVIFEWSYYVKHLDEIIAIWNGGIAIYGGLITGALVLL
                     AYCYNKVLNPIHFLDIAAPSVMVAQAIGRWGNFINQEAYGKAVSQLNYLPSFIQKQMF
                     IEGSYRIPTFLYESLWNLLGFVIIMMWRRKPKSLLDGEIFAFYLIWYGSGRLVIEGMR
                     TDSLMFLGIRISQYVSALLIIIGLIFVIKRRRQKGISYYQE"
     gene            18280..18687
                     /locus_tag="ACGEUI_000017"
     CDS             18280..18687
                     /locus_tag="ACGEUI_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFF8164900.1"
                     /translation="MDLVGISLMIIALAFVALVIFLIIVLKKVSETIDEAKKTISVLT
                     SDVNVTLHQTNDILAKANILVEDVNGKVATIDPLFVAIADLSESLSDLNSQARHFGQK
                     ATNATGNVSKAGKLALVGKVASKVFGKKGEKHE"
     gene            18680..19108
                     /locus_tag="ACGEUI_000018"
     CDS             18680..19108
                     /locus_tag="ACGEUI_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFF8164901.1"
                     /translation="MNKSFKNLVIGAVSGVAAAYFLSTEKGKALKNRAEKAYQAYKES
                     PDDYHQFAKEKGSEYSHLARDTFYDVKDKLASGDLTKEDMLDLLKDKTTAFVQKTKET
                     LAEVEAKEKQDDVIIDLNEEDIIIDYTEQDEPVSDTLDKH"
     gene            complement(19267..19551)
                     /locus_tag="ACGEUI_000019"
     CDS             complement(19267..19551)
                     /locus_tag="ACGEUI_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HFF8164902.1"
                     /translation="MPAPLKKHNSVEQDDSYQESQSKFRDFQEFNGQSAKLKELVFFA
                     RIASFSLITVLFAFVLLVMNLAPMFAFLFASIISLGITSLFAKVIKSLLM"
     gene            19750..20676
                     /locus_tag="ACGEUI_000020"
     CDS             19750..20676
                     /locus_tag="ACGEUI_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFF8164903.1"
                     /translation="MEKIIITATAESIEQVKALLAAGVDRIYVGEANYGLRLPHNFSY
                     DELRQIAKLVHDAGKELTVACNALMHQDMMDQIKPFLDLMIEIAVDYLVVGDAGVFYV
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVESVLAREIPSAELFTLAENLEFPAE
                     VLVYGASVIHHSKRPLLENYYHFTKIDDEVSRERGLFLAEPGDASSHYSIYEDNHGTH
                     IFINNDIDMMSKLGELYAHGLTHWKLDGIYCPGDDFVAITKLFIQAKTLLEAGQFTQE
                     EAEKLDQAVHAHHPAGRGLDTGFYEFDPKTVK"
     gene            20767..22053
                     /locus_tag="ACGEUI_000021"
     CDS             20767..22053
                     /locus_tag="ACGEUI_000021"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFF8164904.1"
                     /translation="MSHMKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGIDYAHARGAKVYVAANMVTHEGNEIGAGEWFRQLRDMGLDAVIVSDP
                     ALIVICSTEAPGLEIHLSTQASSTNYETFEFWKAMGLTRVVLAREVNMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGGERRSL
                     KGEIPEDYSMSSVDMCMIDHIPDLIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFYAIKEELIDELWKVAQRELATGFYYGIPTENEQLFGARRKIPQYKFVGEVV
                     AFDSASMTASIRQRNVIMEGDRIECYGPGFRHFETVVKDLHDADGQKIDRAPNPMELL
                     TISLPREVKPGDMIRACKEGLVNLYQKDGTSKTVRT"
     gene            22263..22475
                     /locus_tag="ACGEUI_000022"
     CDS             22263..22475
                     /locus_tag="ACGEUI_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HFF8164905.1"
                     /translation="MAEFTFNIEEHLLTLSENDKGWTKELNRVSFNGAEAKWDIRTWS
                     PDHTKMGKGITLTNEEFKTILDAFRK"
     gene            complement(22572..22712)
                     /locus_tag="ACGEUI_000023"
     CDS             complement(22572..22712)
                     /locus_tag="ACGEUI_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8164906.1"
                     /translation="MTKEYISYISAAIFLLYGLLAAKWIFLGLGLILVLVGLADRLNS
                     KK"
     gene            complement(22848..24341)
                     /gene="lysS"
                     /locus_tag="ACGEUI_000024"
     CDS             complement(22848..24341)
                     /gene="lysS"
                     /locus_tag="ACGEUI_000024"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFF8164907.1"
                     /translation="MSNQHIEELNDQQIVRREKMTALAEQGIDPFGKRFDRTANSAEL
                     KEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGFAHLQDREGQIQLYVRKDSVGED
                     NYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETI
                     YRKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFV
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYLDIMNLTEGIIQHAAKAVRGDGPIDYQGTEIRINEPFKRVHMVDAIKEVTGAD
                     FWPEMTVEEAIALAKEKQVPLEKHFISVGHIINAFFEEFVEETLVQPTFVFGHPVEVS
                     PLAKKNPEDTRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATG
                     IDYDFVEALEYGMPPTGGLGIGIDRLCMLLTNTTTIRDVLLFPTMKP"
     gene            24515..25417
                     /locus_tag="ACGEUI_000025"
     CDS             24515..25417
                     /locus_tag="ACGEUI_000025"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8164908.1"
                     /translation="MITAIVFDVDDTIYDQQAPYRIAMEKCFPDFDMSVMNQAYIRFR
                     HYSDVGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEAAGVHFQEVYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVRKLGLYDYIDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPQTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRQLKPGTKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDVNDKKNPILQMGINNGLMMAAERLLESNMS
                     IDKVVILLRLTKQQEKVLRLKYAR"
     gene            complement(25525..26148)
                     /locus_tag="ACGEUI_000026"
     CDS             complement(25525..26148)
                     /locus_tag="ACGEUI_000026"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8164909.1"
                     /translation="MKLYFVRHGKTLWNLEGRFQGAGGDSPLLEEAKDEIHLLGKELA
                     KVAFDAVYTSDLQRAMATAAIILDAFDQQPKLYHTDQLREWRLGKLEGAKIATMAAIY
                     PQQMLAFRENLAQFKPDQFEAESIYQTTQRVCHLIQSFKDKHYQNVLIVGHGANLTAT
                     IRSLLGFEPALLLAKGGLDNASLTILETKDYLTYDCLIWNDKSFLEV"
     gene            complement(26489..26968)
                     /locus_tag="ACGEUI_000027"
     CDS             complement(26489..26968)
                     /locus_tag="ACGEUI_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFF8164910.1"
                     /translation="MAKKTKLKKTLVEQILDKANIAHQGLKLNALEGDFPDDLQPSDI
                     YKTLALTGDQTGPLIGIIPLTEHLSEKQLAKVSGNKKVSMVPQKDLQKTTGYIHGANN
                     PVGIRQKHSYPIFIDQTALEKGQIIVSAGEVGRSIKISSQALADFVGASFADLKKRK"
     gene            complement(27059..27622)
                     /gene="thiT"
                     /locus_tag="ACGEUI_000028"
     CDS             complement(27059..27622)
                     /gene="thiT"
                     /locus_tag="ACGEUI_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFF8164911.1"
                     /translation="MSPNTNVKYLIEAAIFAALAMTLSFIPDFAGWFSPSYGAIALVI
                     FSLRRGLKYGMLAGLIWGLLHFVLGKVYYLSMSQVFIEYILAFTSMGLAGSFSNSLIK
                     TLRRQQTFFAVFLAIMASLLAVTVRYLWHFLAGIIFWGSYAPKGMSAVWYSFSVNGTA
                     GVLTFLITCLALMIALPIHPQLFDPKD"
     regulatory      complement(27667..27763)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(27891..28739)
                     /locus_tag="ACGEUI_000029"
     CDS             complement(27891..28739)
                     /locus_tag="ACGEUI_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFF8164912.1"
                     /translation="MRRKIKPIVVLVFFILLAMVLIIGKRQANHAKQKEIEDAKSHIP
                     IATSNPGKAKTSTSETEDFILNPIVDVSGWQLPEEIDYDTLSRHISGAIVRVYGGSQI
                     TAHNNAAFTTGIDKSFKTHIKEFQKRNVPVAVYSYALGRSTKEMKEEARAFYKNAAPY
                     NPTYYWIDVEEATMKDMNKGVTAFREELKKLGAENVGLYIGTYFMAEQDISTKGFDSV
                     WIPTYGSDSGYYEAAPNTTLDYDLHQYTSQGYLSGFNNALDLNQIAVTKDTKKTFEKL
                     FGNSNN"
     gene            29064..29567
                     /locus_tag="ACGEUI_000030"
     CDS             29064..29567
                     /locus_tag="ACGEUI_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8164913.1"
                     /translation="MSAKLMSRIAIMSALCVVLRMVFSSLPNVQPVTAFLLSYLLYFG
                     LAEAVLVMMLCLFLSAFLLGFGPWVFWQVTCFVLVLLLWRFVLYPLSQQFPKYQLGCQ
                     AFLVALCGLLYGVLIDTCFAYLYSMPWWSYVLAGMPFNIAHALSTLVFFPVVMMLFRR
                     LIGEQKI"
     gene            29551..29937
                     /locus_tag="ACGEUI_000031"
     CDS             29551..29937
                     /locus_tag="ACGEUI_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFF8164914.1"
                     /translation="MNKRFKTGFLALVAMLLVACSQGTKQIQTTPSVPKADHHVRLVV
                     KEDTNTVDEKVSFGKGDTVLEVLKDNYEVKEKDGFITAIDGIEQDTKANKYWLFKVNG
                     KMADKGADQITVKDGDSIEFYQEVFN"
     gene            complement(29983..30462)
                     /locus_tag="ACGEUI_000032"
     CDS             complement(29983..30462)
                     /locus_tag="ACGEUI_000032"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFF8164915.1"
                     /translation="MPNLYDFTVKAQDGSDISLVDYKEKVVLVVNTATKCGLTPQYQA
                     LQALYDTYHDKGFEVLDFPCNQFLNQAPGDAEEINHFCSLTYHTTFPRFAKIKVNGKD
                     ADPLFTWLKEEKSGPLGKRIEWNFTKFLIDQNGQVIKRYSSKTDPKLIEEDLKALLG"
     gene            complement(30455..32254)
                     /gene="pepF"
                     /locus_tag="ACGEUI_000033"
     CDS             complement(30455..32254)
                     /gene="pepF"
                     /locus_tag="ACGEUI_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFF8164916.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKALQDIDLFKRN
                     YEGRLTSVDDFTQALIEIEHIYIQMSHIGTYAFMPQTTDFSDESFAQIAQAGDDFMTK
                     ASVALSFFDTALANADLDVLDTLEKNPYFSAAIRMAKIQKEHLLSPDVEKALANLREV
                     INAPYDIYTKMRAGDFDMDDFEVDGKTYKNSFVSYENFYQNHENAEIREKAFRSFSKG
                     LRKHQNTAAAAYLAKVKSEKLLADMKGYASVFDYLLAEQEVDRSLFDRQIDLIMTEFG
                     PVAQKFLKHVAQVNGLEKMTFADWKLDIDNDLNPEVSIDGAYDLVMKSLAPLGQEYTK
                     EIERYQTERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNTHMSTYYVEAPSTFNELMLSDYLEHQFDDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGGTFGADQLNAMMKEVLTDFWGDAVDIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLNLKHNPNGAKEWLDFLKSGGSRTPLDTAMLIG
                     ADIATEKPLRDTIQFLSDTVDQIISYTEDMSHA"
     gene            32449..35211
                     /gene="ppc"
                     /locus_tag="ACGEUI_000034"
     CDS             32449..35211
                     /gene="ppc"
                     /locus_tag="ACGEUI_000034"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFF8164917.1"
                     /translation="MPLKKLESSNNQDIIAEEVALLKEMLENITRRMIGDDAFTVIES
                     IMVLSEKQDYIELEKVVANISNQEMEVISRYFSILPLLINISEDVDLAYEINYQNNTD
                     TDYLGKLALTIKDLAGKDNGKDILEQVNVVPVLTAHPTQVQRKTILELTTHIHKLLRK
                     YRDAKAGVINLEKWRQELYRYIEMIMQTDIIREKKLQVKNEIKNVMQYYDGSLIQAVT
                     KLTTEYKNLAQKHGLELDNPKPITMGMWIGGDRDGNPFVTAETLCLSATVQSEVILNY
                     YIDELAALYRTFSLSSTLVQPNSEVERLASLSQDQSIYRGNEPYRRAFHYIQSRLKQT
                     QIQLTNQPAARMSSSVGLNTSAWSSPASLENPILAYDSPVDFKADLKAIEQSLLDNGN
                     SALIEGDLREVMQAVDIFGFFLASIDMRQDSSVQEACVAELLKGANIVDDYSSLSETE
                     KCDVLLQQLMEEPRTLSSAAVAKSDLLEKELAIYTTARELKDKLGEEVIKQHIISHTE
                     SVSDMFELAIMLKEVGLVDQQRARVQIVPLFETIEDLDNARDIMAAYLSHDIVKSWIA
                     TNRNYQEIMLGYSDSNKDGGYLASGWTLYKAQNELTAIGEEHGVKITFFHGRGGTVGR
                     GGGPSYDAITSQPFGSIKDRIRLTEQGEIIENKYGNKDVAYYHLEMLISASINRMVTQ
                     MITDPNEIDSFREIMDSIVADSNIIYRKLVFDNPHFYDYFFEASPIKEVSSLNIGSRP
                     AARKTITEITGLRAIPWVFSWSQNRIMFPGWYGVGSAFKRYIDRAQGNLERLQHMYQT
                     WPFFHSLLSNVDMVLSKSNMNIAFQYAQLAERQDVRDVFYEILDEWQLTKNVILAIQD
                     HDALLEDNPSLKHSLKSRLPYFNVLNYIQIELIKRWRNNQLDENDEKLIHTTINGIAT
                     GLRNSG"
     gene            35381..36655
                     /locus_tag="ACGEUI_000035"
     CDS             35381..36655
                     /locus_tag="ACGEUI_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFF8164918.1"
                     /translation="MKIDKRHLLNYSILLPYLILSVIGLIMVYSTTSVSLIQAHANPF
                     KSVINQGVFWIISLVAITFIYKLKLNFLTNTRVLTVVMLGEAFLLIIARFFTTAIKGA
                     HGWIVIGPVSFQPAEYLKIIMVWYLALTFAKIQKNISLYDYQALTRRKWWPTQWNDLR
                     DWRVYSLLMVLLVAAQPDLGNASIIVLTAIIMFSISGIGYRWFSAILVMITGLSTVFL
                     GTIAVIGVERVAKIPVFGYVAKRFSAFFNPFHDLTDSGHQLANSYYAMSNGGWFGQGL
                     GNSIEKRGYLPEAQTDFVFSVVIEELGLIGAGFILALVFFLILRIMNVGIKAKNPFNA
                     MMALGVGGMMLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVGFVLNIDAS
                     EKRDDIFKEAELSYRKDTRKENSKVVNIKQFQ"
     gene            37009..38205
                     /gene="tuf"
                     /locus_tag="ACGEUI_000036"
     CDS             37009..38205
                     /gene="tuf"
                     /locus_tag="ACGEUI_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFF8164919.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETATRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAG
                     DNVGILLRGVQRDEIERGQVIAKPSSINPHTKFKGEVYILSKDEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTINVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            38446..39204
                     /gene="tpiA"
                     /locus_tag="ACGEUI_000037"
     CDS             38446..39204
                     /gene="tpiA"
                     /locus_tag="ACGEUI_000037"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFF8164920.1"
                     /translation="MSRKPIIAGNWKMNKNPQEAKAFVEAVASKLPSTDLVDVAVAAP
                     AVDLVTTIEAAKDSVLKVAAQNCYFENTGAFTGETSPKVLAEMGADYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGLTPIVCCGESLETYEAGKAVEFVGAQVSAALAGLSAEQV
                     ASLVLAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVKDYMACPDVDGALVGGASLEADSFLALLDFLN"
     gene            complement(39303..40538)
                     /locus_tag="ACGEUI_000038"
     CDS             complement(39303..40538)
                     /locus_tag="ACGEUI_000038"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HFF8164921.1"
                     /translation="MALIEISQEQFDHYCHSLVHHSFIQTSEMASLMAKRGAKPQFLG
                     LEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPEELEHFYTQLKDYAKQKDVIELI
                     LKPYDNYQSFDTDGIPISRPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGI
                     DSSRLTRSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSY
                     YQDFYDSFGDSCEFMVATLNFEDYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNR
                     LKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTEQEAVYLFSGSYPKFNKF
                     YSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFRCY
                     PFPIKYHFIRLAKKLLNRY"
     gene            complement(40525..41751)
                     /locus_tag="ACGEUI_000039"
     CDS             complement(40525..41751)
                     /locus_tag="ACGEUI_000039"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HFF8164922.1"
                     /translation="MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWGKVKDNWKHERIS
                     FYENGVQVAAAACLIRKLPLGFTMIYIPRGPIMDYANFELLDFVIKTLKTFGKSKRAL
                     FIKIDPSLVIKQTLEGKESKENDVTLSIIAFLKKLGVEWSGRTKELEDTIQPRIQANI
                     YAKDFDFDSLPKKAKQSIRTATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKS
                     YYQKLLGIYAGQSYITMASLDLPEQKKLLIQQLDKALAEQARLTDKSKPSKVAENQKT
                     IARLQKDLTILSEQLATGQTRIPLAATLTLIYGETSENLYAGMDDDYRNYQAPLLTWY
                     ETAKEAFKRGCRWHNLGGVENQQDGGLYHFKARLNPTIEEFAGEFNIPVGLVSSLAIL
                     TYNLRKKLRSTTNGTN"
     gene            complement(41751..42560)
                     /gene="yidA"
                     /locus_tag="ACGEUI_000040"
     CDS             complement(41751..42560)
                     /gene="yidA"
                     /locus_tag="ACGEUI_000040"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFF8164923.1"
                     /translation="MSIKLVAVDIDGTLLTDDRRITDDVFQAVQEAKAQGVHVVIATG
                     RPIAGVISLLEQLELNHKGNHVITFNGGLVQDAETGEEIVKELMTYDDYLETEFLSRK
                     LGVHMHAITKEGIYTANRNIGKYTVHESTLVNMPIFYRTPEEMTNKEIIKMMMIDEPD
                     LLDAAIKQIPQHFFDKYTIVKSTPFYLEFMPKTVSKGNAIKHLAKKLGLDMSQTMAIG
                     DAENDRAMLEVVANPVVMENGVPELKKIAKYITKSNNDSGVAHAIRKWVLN"
     gene            complement(43016..44317)
                     /locus_tag="ACGEUI_000041"
     CDS             complement(43016..44317)
                     /locus_tag="ACGEUI_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8164924.1"
                     /translation="MNEKVFRDPVHNYIHIDNPLIYDLINTKEFQRLRRIKQVPTTAF
                     TFHGAEHSRFSHCLGVYEIARRVTAIFEEKYADIWNKDESLVTMTAALLHDIGHGAYS
                     HTFEVLFHTDHEAFTQEIITNPETEINAILVRHAPDFPDKVASVINHTYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYFSAANYGQFDLMRILRVIRPVEDGIVFEHSGMHAVEDYIVS
                     RFQMYMQVYFHPASRAVELILQNLLKRAQHLYPEQQAYFQKTAPGLIPFFEKKANLAD
                     YIALDDGVMNTYFQVWMASEDHILSDLASRFINRKILKSVTFDQDSQGELERLRQLVE
                     SVGFDPDYYTGIHINFDLPYDIYRPELENPRTQIEMMQKDGSLAELSQLSPIVKALTG
                     TTYGDRRFYFPKEMLELDDLFAPSKETFMSYISNGHFHFSQ"
     gene            44399..44785
                     /locus_tag="ACGEUI_000042"
     CDS             44399..44785
                     /locus_tag="ACGEUI_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFF8164925.1"
                     /translation="MKLQLTNHIRFGDETEIIQEIHDCEWREKGGYQYLIYQNTDKEK
                     VVIKYNETELTMSRFSNPQSIMKFFAGKKVLIALPTPMGVQQFLTDTSHYHLDCSCQK
                     LDLHYHLLQAQTEMLFASYHLELSWD"
     gene            45016..47697
                     /locus_tag="ACGEUI_000043"
     CDS             45016..47697
                     /locus_tag="ACGEUI_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8164926.1"
                     /translation="MSKEQRHEAFYTQSEETVLAQLETSREGLTSAQAKERLAEYGRN
                     ELDEGEKRSLFMKFLDQFKDLMIIILIVAALLSVLTEGMEGLTDAIIILAVVILNAAF
                     GVYQEGQAEAAIEALKSMSSPLARIRRDGHVTEIDSKELVPGDIVLLEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSTAVSEDAGIGDRVNMGYQNSNVTYGRGIGVIT
                     NTGMYTEVGHIAGMLANADETDTPLKQNLDNLSKILTYAILVIAAVTFAVGVFLRGQH
                     PLEGLMTSVALAVAAIPEGLPAIVTVVLSLGTQVLAKRNAIIRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGTLQSSSADIAFDNTTLRVMNFANDTKVDPSGKLIGDP
                     TETALVEFGLDHNFDVREAMVAEPRVAELPFDSDRKLMSTIHKQADGKYFIAVKGAPD
                     QLLKRVTQIEENGQIRPITDADKKTILDTNKSLAKQALRVLMMAYKYSDALPTLETEI
                     VEANLVFSGLVGMIDPERPEAAQAVKVAKEAGIRPIMITGDHQDTAKAIAKRLGIIEE
                     DGVDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNEGKVVAMTGDG
                     VNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKTI
                     QYLLSANMAEVFTIFLATLFGWDVLQPVHLLWINLVTDTLPAIALGVEPAEPGVMKHK
                     PRGRKSSFFDGGVKEAILYQGAFQTILVLGVYGFALMFPEHTSYHDVHADALTMAYVT
                     LGLIQLVHAYNVKSVYQSIFTVGLFKNKLFNYSIPVAFVALMATVVVPGFNQFFHVTH
                     LTITQWLVVIIGSLLMVVLVELVKAVQRSLGQDEKAI"
     gene            complement(47781..48776)
                     /locus_tag="ACGEUI_000044"
     CDS             complement(47781..48776)
                     /locus_tag="ACGEUI_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8164927.1"
                     /translation="MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRISESI
                     KATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQIVKGIESKAQEFGYQIIIGNSN
                     YDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
                     KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMI
                     IDENQTSSEAIAQFLQGSLTKKSLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNI
                     EWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIFDCQVNWQESTF"
     gene            complement(48840..50747)
                     /locus_tag="ACGEUI_000045"
     CDS             complement(48840..50747)
                     /locus_tag="ACGEUI_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HFF8164928.1"
                     /translation="MFFAFARFKETVNPDFCRNYLLDYQTDSYADQKRIADLLLTNTF
                     LFEDNWDMEPCHIPYHLDPITWQEAVIDDPEWNFMLNRQTYLQKLILVYLVERDERYL
                     LTAKGFILNWIESAIPLDPKGLATRTLDTGIRCFAWVKCLIYLNLFNALTKQEESLIL
                     ASLEKQLQFLHANYLDKYSLSNWGILQTTAILLADAYFGSDLDIAAATAFARKELTQQ
                     IALQILEDGSQFEQSTMYHVEVLKALLELTALVPDYLPQLRPTLLAMSDYLLKMTGPD
                     HKQIPLGDSDVTDTRDILTLAATILEEPHLKAAAFPTLDIDSLLLLGEKGVHTFEQLP
                     VQTLPTFAHHFEHSGHITINQENYYLFFKNGPIGSSHTHSDQNSLCLYYKGQPLFCDA
                     GRYTYKEEPLRYALKSASHHSTAFLEEQLPEQIDSSWAYLSYPKSNYCHLRQNGHVYF
                     IEGSYQTQFSDRNNYQHDRQILILPPGIFLIIDTIQAQGNHCLVSQFILDNHLDVKTD
                     HLSDLRLISDCPFTIEETILSKKYNQYLTSHKLIKRKPFKDKGCTSTLLVPDDTKVTP
                     LTPLQTGKRNPIETALSWHLKGKQFDYSICVLQEDLIKGEKLVLLNSHKIRGKVVVIN
                     HITNEIIRLKH"
     gene            complement(50834..51655)
                     /locus_tag="ACGEUI_000046"
     CDS             complement(50834..51655)
                     /locus_tag="ACGEUI_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8164929.1"
                     /translation="MTGSNKLTKKDYLKTALRAFFLQNGFNYNNYQGIGYANVIYPAL
                     KKHFGSDKKGLYQALEDNCEFYNTNPHFLPFITSLHLVMLENNRPEEETRNIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLASDGLVLGPILFFLAMNIILTAIKIGSGLYGYK
                     VGTSFIDKLSEQMAVVSRMANIVGVTVIAGLAATSVKITVPITFAAGKVDAANTAQKF
                     VTIQGMLDKIAPALLPALFTLLMYYLIKNKKWTTYKLVILTVIIGVIGSWLGILA"
     gene            complement(51642..52424)
                     /locus_tag="ACGEUI_000047"
     CDS             complement(51642..52424)
                     /locus_tag="ACGEUI_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8164930.1"
                     /translation="MDINLLQALLIGLWTAFCFSGMLLGIYTNRCIILSFGVGIILGD
                     LPTALSMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVAIQFLQTFAYTAFAGAPETAKKQLQKGNIRGFKFAANGTIWAFAFIGLGLGLLG
                     ALSMDTLLHLVDYIPPVLLNGLTVAGKMLPAIGFAMILSVMAKKELIPFVLIGYVCAA
                     YLQIPTIGIAIIGIIFALNEFYNKPKQVDATTVQGGQQDDWI"
     gene            complement(52443..52931)
                     /locus_tag="ACGEUI_000048"
     CDS             complement(52443..52931)
                     /locus_tag="ACGEUI_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8164931.1"
                     /translation="MTQPNIIMTRVDERLIHGQGQLWVKFLNCNTVIVANDAVSEDKI
                     QQSLMKTVIPSSIAIRFFSIQKVIDIIHKASPAQSIFIVVKDLQDAKLLVEGGVPITE
                     INIGNIHKTDDKVAITQFISLGETDKSAIRCLAHDHHVVFNTKTTPAGNSASDVDILD
                     YI"
     gene            complement(52967..54166)
                     /locus_tag="ACGEUI_000049"
     CDS             complement(52967..54166)
                     /locus_tag="ACGEUI_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8164932.1"
                     /translation="MARPLKTIALEPIKQPERFTKEDFLSQEDITQALDLALKQVRLN
                     MDYFKEDFPTPATKDNQYAIMDNTEWTNAFWTGCLWLAYEYSGDDAIKALAQANDLSF
                     LDRVTRDIELDHHDLGFLYTPSCMAEWKLLKTPESREAALKAADKLVQRYQDKGGFIQ
                     AWGELGKKEDYRLIIDCLLNIQLLFFASQETGDNRYRDMAINHFYASANHVIRDDASA
                     YHTFYFDPETGDPVKGVTRQGYSDDSAWARGQAWGIYGIPLTYRFLKEPELIQLFKGM
                     THYFLNRLPKDQVSYWDLIFGDGSEQSRDSSATAIAVCGIHEMLKTLPDHDPDKKTYE
                     AAMHSMLRALIKDYANKDLKPGAPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALLRFY
                     KDWNPYW"
     gene            complement(54166..54603)
                     /locus_tag="ACGEUI_000050"
     CDS             complement(54166..54603)
                     /locus_tag="ACGEUI_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIA"
                     /protein_id="HFF8164933.1"
                     /translation="MIAIIVMGHGHFASGIVSALELIAGKQEKVTAIDFTTEMTAADV
                     QDQLSRALIPEEETLVLCDLLGGTPFKVAATLMESLPNTTCNVLSGLNLAMLIEASFA
                     RQTAASFDDLVSGLITCSKEGIVDWKTLSQQEDGATDDELGGI"
     gene            54939..55733
                     /locus_tag="ACGEUI_000051"
     CDS             54939..55733
                     /locus_tag="ACGEUI_000051"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFF8164934.1"
                     /translation="MENMFSLQGKIALITGASYGIGFEIAKAYAQAGATIVFNDIKQE
                     LVDKGLAAYRELGIEAHGYVCDVTDEAGIQQMVSQIEDEVGAIDILVNNAGIIRRTPM
                     LEMAAEDFRQVIDIDLNAPFIVSKAVLPSMIAKGHGKIINICSMMSELGRETVSAYAA
                     AKGGLKMLTKNIASEFGEANIQCNGIGPGYIATPQTAPLRERQADGSRHPFDQFIIAK
                     TPAARWGTTEDLAGPAVFLASDASNFVNGHILYVDGGILAYIGKQP"
     gene            55758..56399
                     /locus_tag="ACGEUI_000052"
     CDS             55758..56399
                     /locus_tag="ACGEUI_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFF8164935.1"
                     /translation="MKIALINENSQAAKNGIIYDALTTVTDKHGYQVFNYGMYGTEGE
                     SQLTYVQNGLLASILLTTKAADFVVTGCGTGVGAMLALNSFPGVTCGFASEPTEAYLF
                     SQINGGNALSIPFSKGFGWGAELNLTLIFERLFAEPMGGGYPKERAIPEQRNARILSD
                     LKKITYRDLLAIVKDIDQDFLKETISGAHFQEYFFANAEPSELVTYLKSVLEQ"
     gene            56428..57429
                     /locus_tag="ACGEUI_000053"
     CDS             56428..57429
                     /locus_tag="ACGEUI_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFF8164936.1"
                     /translation="MSKLLLVGEPLIRVSPNQFQPLTNACEAQLFFGGSEVNIARTLG
                     GFGLEARLFTALPDNPVGHAFHQFLKQSGVDMSLTAWQGHRVGLYYLENGFGCRASQV
                     YYDRCGSSFSALDKDSLDLAAIFEGISHFHFSGISLALGKKTQDLIEVLVREAKKRDI
                     CISFDLNFRSSMIAVADAKRLFSHFAQYADIIFGMEPLLLDSDDFDMFDRKKADTTTI
                     RERLAGLYQRYQLQAIYHTERSNDAQGSNHFKAYAYDRQFYESCEVTTPVLQRVGSGD
                     AFVAGLLYQLLEGNEKQRNLDFAVATASLKCTVAEDQLYHSVQQVEAVLANQRDIQR"
     gene            57434..58069
                     /locus_tag="ACGEUI_000054"
     CDS             57434..58069
                     /locus_tag="ACGEUI_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFF8164937.1"
                     /translation="MGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEV
                     TYTNPFASEVIGQLAERFKEDPEVLIGAGTVLDDVTARQAILAGAQFIVGPNFNRAVA
                     LICHRYSIPYLPGCMTVNEVVTALESGVDMVKIFPGSTVGISFIRAIKSPLPQVEVMV
                     TGGVSSDNLKDWLAAGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ"
     gene            58365..59015
                     /locus_tag="ACGEUI_000055"
     CDS             58365..59015
                     /locus_tag="ACGEUI_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8164938.1"
                     /translation="MIKGIIFDMDGVLFDTEPFYLRRREDFFKTKGIPIDHLNSKDFI
                     GGNLQELWKELLGKNRDDAIVKAITTDYDAYKQAHKPPYQKLLITEVNSCLEQLEKQG
                     IKLAVASNSKRQDVLLALETTQIKDYFEIILAREDVSRGKPYPDIYNKAVQKLGLQKK
                     QLLVVEDSQKGIAAAKAANLTVFAITDYRYGIDQSQADHKIDHLGQLCVKIGCFGS"
     gene            59707..60831
                     /gene="queG"
                     /locus_tag="ACGEUI_000056"
     CDS             59707..60831
                     /gene="queG"
                     /locus_tag="ACGEUI_000056"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFF8164939.1"
                     /translation="MTIKAEIKALAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRIYTERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPSSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNSPATEIDPELAQPELIPFLSLSN
                     GKFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILAFMSHLTLKDEDSRKELELIRHKWQF"
     gene            60900..61998
                     /gene="prfB"
                     /locus_tag="ACGEUI_000057"
     CDS             join(60900..60971,60973..61998)
                     /gene="prfB"
                     /locus_tag="ACGEUI_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFF8164940.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENHMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKI
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            62017..62709
                     /gene="ftsE"
                     /locus_tag="ACGEUI_000058"
     CDS             62017..62709
                     /gene="ftsE"
                     /locus_tag="ACGEUI_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFF8164941.1"
                     /translation="MALIEMSGVTKKYRRSTTALRDVNVSVNQGEFVYLVGPSGAGKS
                     TFIKLLYREEQLTTGKLYVGEFNLTKLKARDVPILRRHIGVVFQDYKLLPRKTVFENV
                     AYAMEVIGEKRRHIKKRVPEVLDLVGLKHKMRSFPSQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEISWEIMQLLERINVQGTTILMATHNSHIVNTFRHRVVAIEDGRIV
                     RDEEKGDYGYDD"
     gene            62702..63631
                     /gene="ftsX"
                     /locus_tag="ACGEUI_000059"
     CDS             62702..63631
                     /gene="ftsX"
                     /locus_tag="ACGEUI_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFF8164942.1"
                     /translation="MIRYFFRHIWESIKNLKRNFWMTFASVSMVAVTLTLVGVFAATL
                     LNIQRVASGVENNVHINTYLQVDSTDAAKVIQNTAGEPVNNDNYHSVYDKIAQIKGVK
                     KITFSSKDEQLKKLQETLGDVWNMYDQDTNPLQDIYLIETQTPKQVKAITKKIRTIEG
                     VEAADYGGINSDKLFKFSTLIQTWGLIGTAMLLFVAVFLISNTIRMTIMSRKRDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGLLGAVLPSLLIYYGYDLVYKHFAQELQRNNLSMYPL
                     DPYVYYLIGALFVIGIMIGSLGSVLSMRRYLKF"
     gene            complement(63941..64576)
                     /locus_tag="ACGEUI_000060"
     CDS             complement(63941..64576)
                     /locus_tag="ACGEUI_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8164943.1"
                     /translation="MKIYKTINHIAGENTYYLVNDQAVILIDPGSNGQEIIAKIKSFE
                     KPLVAILLTHTHYDHIFSLDLVRDAFDHPPVYVSEKEAAWLSSPDDNLSGLGRHDDII
                     TVIARPAENFFKLKQPYQLNGFEFTVLPTSGHSWGGVSFVFHSDELVVTGDALFRETI
                     GRTDLPTSNFEDLITGIRQELFTLPNHYRVYPGHGPSTTICHEKNANPFFH"
     gene            64807..65571
                     /locus_tag="ACGEUI_000061"
                     /pseudo
     CDS             64807..65571
                     /locus_tag="ACGEUI_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0019152 - acetoin dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045150 - acetoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
     gene            65721..68180
                     /locus_tag="ACGEUI_000062"
     CDS             65721..68180
                     /locus_tag="ACGEUI_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HFF8164944.1"
                     /translation="MTQKKLRKYAVVDLEATGAGPNASIIQVGIVIIQGNKIIDSYET
                     DVNPHESLDEHIVHLTGITDKQLAKAPDFGQVAHHIYQLIEDCIFVAHNVKFDANLLA
                     EALFLEGYELTIPRVDTVELAQLFFPRFEKYNLSHLSRQLNIDLAEAHTAIADARATA
                     ILFLRLLQKIESLPIECLESLLVYSDSLLFETAMVIQEGLAKAKPYDPNKYIKIRQIL
                     LPKGSKALKPYQISKSFPINMALLGLEERPKQTQFAQLIDEDYHQGVASFIEAQTGIG
                     KTYGYLLPLLAKEDQNQIIVSVPTKLLQDQLMAGEVAAIQEQFHIACHSLKGPANYLK
                     LDSFADSLDQNDQNRLVNRYKMQLLVWLLETKTGDLDEIKQKQRFAAYFEQLKHDGDI
                     KQSSEFYDYDFWRVSYEKAKTARLLITNHAYFLHRVQDDKDFARNKVLVFDEAQKLML
                     QLDQLSRHQLNLTVFLQTIQAKLSNPLPLLEKRLLESLSFELGQVSSDYYQNKEHQLA
                     HDWSRIAGYAKELTGADYQELQAFFATSDGDYWLSSEKQEEKRVTYLNSASKAFIHFQ
                     QLLPETVKTYFVSATLTISSEVTLADLLGFEEYLYHVIEKDKKQDQLVLVDQEAPIVT
                     EVSDQIYVEAIAKRIESLKQEGYPILVLFNSKKHLLLVSDYLDQWQVPHLAQEKNGTA
                     YNIKKRFDQGEQTILLGLGSFWEGVDFIQADRMITLIARLPFDNPEDFFVKKMSHYLL
                     EKGKNPFRDYFLPMTILRLKQAIGRTMRRQDQKSVVIILDRRLLTKSYGQVILEGLGQ
                     EFLISQQNFHDCLVETDCFLI"
     gene            68515..69708
                     /locus_tag="ACGEUI_000063"
     CDS             68515..69708
                     /locus_tag="ACGEUI_000063"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8164945.1"
                     /translation="MPKLSKRVLEMKESVTLAAGARAKALKAQGRDVLNLTLGEPDFF
                     TPKHIQDKAIESIQNGTASFYTNASGLPELKAAIATYLKNQYGYHLSPDQIVAGTGAK
                     FILYAFFMAVLNPGDQVLIPTPYWVSYSDQVKMAEGQPIFVQGLEENQFKVTVDQLER
                     ARTSKTKVVLINSPSNPTGMIYGAEELRAIGEWAVHNDILILADDIYGSLVYNGNQFV
                     PISTLSEAIRRQTITVNGVAKSYAMTGWRVGFAAGEPEIISAMSKIIGQTTSNLTTVS
                     QYAAIEAFCGSQSSLEEMRLAFEERLNITYPLLCQVPGFEVVKPQGAFYFFPNVKKAM
                     EMTGFSDVTSFANAILEEVGLAVVSGAGFGAPENVRLSYATDIETLKEAVRRLHVFME
                     SNEIN"
     gene            69729..71075
                     /gene="asnS"
                     /locus_tag="ACGEUI_000064"
     CDS             69729..71075
                     /gene="asnS"
                     /locus_tag="ACGEUI_000064"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFF8164946.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEIIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYATYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYITEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIRP"
     gene            71489..72379
                     /gene="rapZ"
                     /locus_tag="ACGEUI_000065"
     CDS             71489..72379
                     /gene="rapZ"
                     /locus_tag="ACGEUI_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFF8164947.1"
                     /translation="MSDKHINLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALVPK
                     FLELIEQTNENRRVALVVDMRSRLFFKEINSTLDSIESNPSIDFRILFLDATDGELVS
                     RYKETRRSHPLAADGRVLDGIRLERELLSPLKSMSQHVVDTTKLTPRQLRKTISDQFS
                     EGSNQASFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYQVELREKTGLDEDVFNYVM
                     SHPESEVFYKHLLNLIVPILPAYQKEGKSVLTVAIGCTGGQHRSVAFAHCLAESLATD
                     WSVNESHRDQNRRKETVNRS"
     gene            72376..73353
                     /locus_tag="ACGEUI_000066"
     CDS             72376..73353
                     /locus_tag="ACGEUI_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HFF8164948.1"
                     /translation="MKNPKMTVIGGGTGISIILKSLRNEAVDITAVVTVADDGGSSGE
                     LRNAMQLAPPGDLRNVLLAMSDMPKFYERVFQYRFNESDGALAGHPLGNLIIAGISEM
                     QGSTYNAIQILTKFFHITGKIYPSSEQALTLHAVFKDGHEVAGESSIAKYPGMIDHVY
                     VTNTYNDQKPQASRKVVEAILESDMIVLGPGSLFTSILPNLVIPEIKEALRQTKAEVV
                     YICNIMTQYGETEQFSDADHVAVLNQHLGRDLIDTVLVNVAKVPQAYMNSNKFDEYLV
                     QVDHDFAGLCRAAKRVISSYFLRLENGGAFHDGNLVVEELMNLVRIVKQ"
     gene            73350..74261
                     /gene="whiA"
                     /locus_tag="ACGEUI_000067"
     CDS             73350..74261
                     /gene="whiA"
                     /locus_tag="ACGEUI_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFF8164949.1"
                     /translation="MSFTTKVKEELIHLSTGDNNELAAIIKLSGSLGLAHQSLHLSIT
                     TENAKIARYIYSLIEDAYVIVPEIRYHQKTNLRKNRVYTVYVEQGVETILADLKLADS
                     FFGLETGIEPQVLSDDNAGRSYLKGAFLAAGSIRDPESGKYQLEIYSVYLDHAQDLAQ
                     LMQKFMLDAKTIEHKSGAVTYLQKAEDIMDFLIIIGAMSCKEDFEAIKLLREARNDIN
                     RANNAETANIAKTISASMKTINNIIKIMDTIGLESLPIELQQVAQLRVKHPDYSIQQV
                     ADALEFPITKSGVNHRLRKINKIADDL"
     gene            74394..75791
                     /locus_tag="ACGEUI_000068"
     CDS             74394..75791
                     /locus_tag="ACGEUI_000068"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HFF8164950.1"
                     /translation="MACTTILVGKKASYDGSTMVARTEDSQNGDFTPKKMIVVKPEDQ
                     PRHYRSVQSSFEMDLPDNPMTYTSVPDALGKDGIWAEAGVNEANVAMSATETITTNSR
                     VLGADPLVASGIGEEDMVTLVLPYIRSAREGVLRLGAILEDYGTYESNGVAFSDEHDI
                     WWLETIGGHHWIARRVPDDAYVTNPNQFGIDHFEFNNPEDYLCSADLKDFIDTYHLDL
                     TYSHEHFNPRYAFGSQRDKDRQYNTPRAWIMQKFLNPEIVQDPRSFALAWCQKPYRKI
                     TVEDVKYVLSSHYQDTGYDPYGSEGTPVSKKVFRPIGINRTSQTAILHIRPNKPQEIA
                     AIQWMAYGSMPFNTMVPFFTQVKTIPDYFANTYENVFTDNFYWTNRLIAALADPHYNH
                     HETDLDNYLEETMAKGHAMLHAVEAQLLAGETVDLEEENQKMSDYVQGETQTLLNKIL
                     FDASNLMTNRFSLSD"
     gene            75943..77490
                     /locus_tag="ACGEUI_000069"
     CDS             75943..77490
                     /locus_tag="ACGEUI_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFF8164951.1"
                     /translation="MKKKILLMMSLISVFFAWQLTQAKQVLAEGKVKVVTTFYPVYEF
                     TKGVIGNDGDVFMLMKAGTEPHDFEPSTKDIKKIQDADAFVYMDDNMETWVSDVKKSL
                     TSKKVTIVKGTGNMLLVAGAGHDHHHEDADKKHEHNKHSEEGHNHAFDPHVWLSPYRS
                     ITVVENIRDSLSKAYPEKAENFKANAATYIEKLKELDKDYTAALSDAKQKSFVTQHAA
                     FGYMALDYGLNQISINGVTPDAEPSAKRIATLSKYVKKYGIKYIYFEENASSKVAKTL
                     AKEAGVKAAVLSPLEGLTEKEMKAGQDYFTVMRKNLETLRLTTDVAGKEILPEKDTTK
                     TVYNGYFKDKEVKDRQLSDWSGSWQSVYPYLQNGTLDQVWDYKAKKSKGKMTAAEYKD
                     YYTTGYKTDVEQIKINGKKKTMTFVRNGEKKTFTYTYAGKEILTYPKGNRGVRFMFEA
                     KEADAGEFKYVQFSDHAIAPEKAKHFHLYWGGDSQEKLHKELEHWPTYYGSDLSGREI
                     AQEINAH"
     gene            77638..78360
                     /locus_tag="ACGEUI_000070"
     CDS             77638..78360
                     /locus_tag="ACGEUI_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8164952.1"
                     /translation="MPKEQPLYLQIVDDLEVKIKKSMTENDKLLSERELSDLYGVSRI
                     TIRLTLKELELRDLIYKKQGKGTYVSGIKEPATDLSSTYSFTEQMKKMGKTPKTEILS
                     FEQYQVTPYLSGLLELDPDTEVFELERLRIADDMPLMFERSYIPAQPFQGLSIADLKR
                     KALYDIFAKDYQETIRLAEEEFYASIALDYEAGLLGIKKGDPVLHIIRKTYNDKNILI
                     ELTFSIARADQFRYRVQHHPNG"
     gene            78379..79578
                     /locus_tag="ACGEUI_000071"
     CDS             78379..79578
                     /locus_tag="ACGEUI_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8164953.1"
                     /translation="MFTKTQEALEALGAAITTKEIKQEPRLWQETMTFFEETRDSLDS
                     FLKRVCNSANRNNVHVIFTGAGTSEYIGNTICPYLKKVGNRQRYLFESVASTDLVAAP
                     DYYLVEEETVLLVSFARSGNSPESVAAVNLVNQLVPNSYHLTITCAKDGELAKKAQQD
                     ERSYLYLMPEDANDAGFAMTGSFTCMMLAALLIFDDANSIAQKEAYVTDMILAGQAVI
                     DQERRLQELADLGFERLVYLGSAGLAKLTQEAQLKMLELTAGQVATLYESSMGFRHGP
                     KSFINDRTLVIGFVNNDAYVRQYDLDMLEEIQADGIALKILALLQGGDINFSEDQFRL
                     DTRHLLPDAYLAFPMILVAQTLALLTAVTLGNSPDTPSATGTVNRVVKGVTIHPYPTS
                     SRQCNKA"
     gene            complement(79675..79935)
                     /locus_tag="ACGEUI_000072"
     CDS             complement(79675..79935)
                     /locus_tag="ACGEUI_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFF8164954.1"
                     /translation="MRKDIHPDYRPVVFLDTTTGYQFLSGSTKASKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAK"
     gene            complement(80050..80991)
                     /locus_tag="ACGEUI_000073"
     CDS             complement(80050..80991)
                     /locus_tag="ACGEUI_000073"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFF8164955.1"
                     /translation="MITTFETILDKIKAHQTIIIHRHQNPDPDALGSQAGLKEIIAQN
                     FPDKKVLMTGFDEPSLAWISQMDQVTDKDYKEALVIITDTANRPRIDDERYTLGKCLI
                     KIDHHPNDDVYGDFYYVDTSASSASEIIADFAFSQNLTLSDKAAKLLYTGIVGDTGRF
                     LYASTTSKTLSIASQLRHFEFDFAAISRQMDSFPLKIAKLQSYVFEHLTIDESGAAYV
                     LVSQETLKHFDVTLAESSAIVCAPGKIDNVQAWAIFVELTDGNYRVRMRSKEKIINGI
                     AKRHGGGGHPLASGANSANLEENQAIFRELIAVCQEI"
     gene            81385..81834
                     /locus_tag="ACGEUI_000074"
     CDS             81385..81834
                     /locus_tag="ACGEUI_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFF8164956.1"
                     /translation="MALAKIVYASMTGNTEEIADIVANKLQELGHDVDIDECTTVDAS
                     EFENADIAVVATYTYGDGDLPDEIVDFYEDLQDLDLEGKIYGVVGSGDTFYDYFCKSV
                     DDFSEQFALTGAIKGAEPVKVDLAAEDEDIDRLEAFAEQLSQAVNNL"
     gene            82010..82294
                     /locus_tag="ACGEUI_000075"
     CDS             82010..82294
                     /locus_tag="ACGEUI_000075"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFF8164957.1"
                     /translation="MRLEKIRQEINGIDHHLVALLEKRMALVEQVTAYKLANHLPVLD
                     QARENQILDRVSYLVKDQAFEPAIHETFKTIMSLSRQYQTQHLTGGDIND"
     gene            82287..83549
                     /locus_tag="ACGEUI_000076"
     CDS             82287..83549
                     /locus_tag="ACGEUI_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFF8164958.1"
                     /translation="MIKSIATLPKPSLKEVLGLVGLALPIGGAVGVVDVIFGKGLLFL
                     SEYRDHHLFLLLPFLALAGLVIVFLYDKLGKEVRQGMGLVFQVGHGQKNQIPPILIPL
                     ILFSTWVTHLFGASAGREGVAVQIGATISHYCRRFVTSQEAARHLLIMGMAAGFAGLF
                     QTPIAAVVFALEVLLVGTLRYSALLPSLVAAYVASWTSHALGLEKFTIVLEETLTITP
                     LTLVKLIGLGLIFGLVGNSFAYLLGWFKPYLSQKLPNPYFRIAFIGALLSICLMIGHV
                     GRYSGLGTNLIAAAFSGQTILTYDWLLKMIVTVISLSAGFQGGEVTPLFAIGASLGIV
                     LAPYLGLPVLLVAALGYTTVFGSATNTFWAPIFIGIEVFGPENALAYFVTSAAAYMVS
                     HRHSIYSYQKVVMINLKDMTVNQMDLKK"
     gene            83664..84011
                     /gene="rplS"
                     /locus_tag="ACGEUI_000077"
     CDS             83664..84011
                     /gene="rplS"
                     /locus_tag="ACGEUI_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFF8164959.1"
                     /translation="MNPLIQSLTEGQLRSDIPNFRPGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVIRHGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            84258..84329
                     /locus_tag="ACGEUI_000078"
     tRNA            84258..84329
                     /locus_tag="ACGEUI_000078"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84291..84293,aa:Arg,seq:cct)
     gene            complement(84292..84606)
                     /locus_tag="ACGEUI_000079"
                     /pseudo
     CDS             complement(84292..84606)
                     /locus_tag="ACGEUI_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004234471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            84629..84920
                     /locus_tag="ACGEUI_000080"
                     /pseudo
     CDS             84629..84920
                     /locus_tag="ACGEUI_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056811.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
     gene            85023..85583
                     /locus_tag="ACGEUI_000081"
     CDS             85023..85583
                     /locus_tag="ACGEUI_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFF8164960.1"
                     /translation="MNYQDYIWDLGGTLLDNYELSTQAFVQTLAFFSLPGDHDAVYQK
                     LKESTAIAVAMFAPNEPEFLHVYRLREADKLAQPIWCLGAKEILGKIATSGSRNFLIS
                     HRDCQVNQLLEQAGLLIYFTEVVTASNGFARKPNPESLFYLKEKYDINSGLVIGDRLI
                     DKQAGQAAGFNTLLVDGRKNLLEIVT"
     gene            85584..87536
                     /gene="gyrB"
                     /locus_tag="ACGEUI_000082"
     CDS             85584..87536
                     /gene="gyrB"
                     /locus_tag="ACGEUI_000082"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFF8164961.1"
                     /translation="MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEG
                     LHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITVVDDGRGIPVDIQAKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVV
                     ADLEVIGTTDVTGTTVHFTPDPEIFTETTQFDYSVLAKRIQELAFLNRGLKISITDKR
                     SGMEQEEHFLYEGGIGSYVEFLNDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQET
                     VMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPGIDQEDQ
                     LKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVF
                     DMLMGDRVEPRRDFIEENAVYSTLDI"
     gene            87904..89628
                     /gene="ezrA"
                     /locus_tag="ACGEUI_000083"
     CDS             87904..89628
                     /gene="ezrA"
                     /locus_tag="ACGEUI_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFF8164962.1"
                     /translation="MSSGIILLIVAIVLLVIIAYLVGVIIRKRNDSLITSLEERKQAL
                     FALPVNDEIEEVKSLHLIGQSQTSFREWNQKWVDLTVNSFADIENHIFEAENLNDTFN
                     FIRAKHEINSVESQLNLVEEDIASIREALNILKEQEEKNSARVTHALDLYEKLQASIS
                     ENEDNFGSTMPEIDKQMKNIETEFSQFVALNSSGDPVEASEVLDRAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKNIEARFQEIRESIRANSSELV
                     TLDLDRAREENTHIQERIDSLYEVFEREIAAYKVAAKNSKMLPRYLEHVKRNNEQLKD
                     EIARLSRKYILSETESLTVKAFEKDIKEIEDSTLAVAEQFGLQEKPFSELQVTFERSI
                     KTLTNVESGQMDVFAAVKDIEKIESQARHNLDVYVTQLHMIKRYMEKRHLPGIPQDFL
                     SAFFTTSSQLEALMDELSRGRINIEAVSRLSEVATVAIANLEDLTYQVVQNATLTEQL
                     LQYSNRYRSFEAGVQSSFEHALRLFEVENDYQASFDEISYALETVEPGVTDRFVNSYE
                     KTREHIRF"
     gene            complement(89760..90218)
                     /locus_tag="ACGEUI_000084"
     CDS             complement(89760..90218)
                     /locus_tag="ACGEUI_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFF8164963.1"
                     /translation="MTNLEDKLLKGAWGERRLNPEQQRYYLGTYAERVVLSALLDEAQ
                     TKTVKDYLKRELPSLQLVYQPLHLKISSKLAPQLQILYMKLAKENHLPVTIITETHMT
                     SPFAFILHTDHAINLKETRLEVILKQTKNDQLSKQTPEKTKSFWKRFLKK"
     gene            90445..91752
                     /gene="eno"
                     /locus_tag="ACGEUI_000085"
     CDS             90445..91752
                     /gene="eno"
                     /locus_tag="ACGEUI_000085"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-displayed alpha-enolase"
                     /protein_id="HFF8164964.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEGLRWGAEVFHALKKILKERGLVTAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQV
                     DYLEELVNKYPIITIEDGMDENDWDGWKVLTERLGKRVQLVGDDFFVTNTEYLARGIK
                     ENAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            complement(92234..93263)
                     /locus_tag="ACGEUI_000086"
                     /pseudo
     CDS             complement(92234..93263)
                     /locus_tag="ACGEUI_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096501.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(93530..93982)
                     /locus_tag="ACGEUI_000087"
     CDS             complement(93530..93982)
                     /locus_tag="ACGEUI_000087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8164965.1"
                     /translation="MSAEGIVGKLPSYHLLLFTIQLEELLKTYLPPIPVYLLTNNTAA
                     LDLMTNALSIYFPPAIATVMPVNVEIIPFKDIVKEKQSVIIADRQYLNLIQHLYQNQG
                     HLFLYFLFSFRDVSEAYIHKAFLDFRQKRYDEFIVTLLDTYHKNLSSP"
     gene            complement(94183..95070)
                     /locus_tag="ACGEUI_000088"
     CDS             complement(94183..95070)
                     /locus_tag="ACGEUI_000088"
                     /inference="COORDINATES: protein motif:HMM:NF019875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8164966.1"
                     /translation="MLHLHLETKLQDKLSLLNILLDVSEVSIDQLCQETELKKQRVYN
                     LLFEMIKDLEDTLTLTICDDTVSIPYKTYQLKMPYFKKLYQTSIFLKMLCFLIEPGEL
                     SLHDFIKREYISQATAYRIRTNCRKYLKKVGLNVRQNHVVGPEYRIRFLIALLHYQFG
                     MTIYDFDKTSMNKVVSLIINSNQATTLNDASKAPYEFSYFAILISLIWKRRHDNLGIP
                     QTDAFKHLKKLSIYRDIKMTSQEIIGKWYHPELTDEDLDYIFLCFCTTNNPFHKDKWT
                     PKKVKELFELVMTKQMEKP"
     gene            95542..101478
                     /locus_tag="ACGEUI_000089"
     CDS             95542..101478
                     /locus_tag="ACGEUI_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HFF8164967.1"
                     /translation="MFLKHQDVKQKNWRMRKVKKVFVSSCMLLTVGLGVAVPTGFSQS
                     NGVMVVKAAEVPATDLSRQASDSERVDESSLLQKENLSVDSFKLENLNGWEAENDTAG
                     NLGKFKDPDSSGYQNILTSSGKNISVAVAPKGSGKMNIKVTKRSNFQGGYYVGGLRTQ
                     TPVLKLNDVYRYSFTTKKLSGNSSEFKTRVKPVESNNKLGKELVIRVDNKNVSTKHDW
                     LPDISDGTHTVDFTGLDKKLSVAFRFSPRQTSNVVYEFSNINIKNISPASVPAIPSKV
                     LEGTSVLSGTAISSGDTLEKRKSFDGDILRVYKDSKIIARTVIKGNKWDVKLSKPLIA
                     GEKLDFEILHPRSQNVSKKISKQVEAKPFDPASYKEKVIAKLKPVYEATSEKITNDAW
                     LDENAKDLQKQKLEEQYISGKVAISEAGTKQEAIDAAYNKYSSQTDPDSLPSQYKQGN
                     KENEQEKGRQDLIQTRDLTLKAIQEDKWLTEQEKTIQKEEALKAFETGIESVNQTVSL
                     EQLKQRLIVYKASEKDSEKKEYPESIPNQHIPGKEKEVKAAKQEELKKLHDTTLEKIN
                     QDKWLTPDQQAEQLKQAEVTFKKGQEAIKSAQTLTQLETDLADYVSENEGKGNSIPDK
                     YKSGHKDDLVNKAEVKLKEAHEATKQAIEKDPWLSPEQKKAQKEKAKARLDEGLKALK
                     AADSLEILKVTEEAFVDKEKNPDSIPNQHKAGTADQARKQALDSLDKEVQKELESIDN
                     DNTLTTDEKAAAKKKVNDAYDVAKQTAMEANSYEDLTTIKDEFLSNLPHKQGTPLKDQ
                     QSDAIAELEKKQQEIEKAIEGDKTLPRDEKEKQIADSKERLKSDTQKVKDAKNADAIK
                     KAFEEGKVNIPQAHIPGDLNKDKEKLLAELKQKADDTEKAIDVDKTLTEDEKKEQKVK
                     TKAELEKAKTDVKNTQTREELDKKVPELKKAIEDTHVKGNLEGVKNKAIEDLKKAHTE
                     TVAKINGDDTLDKATKEAQVKEADKALAAGKDAITKADDADKVSTAVTEHTPKIKAAH
                     KTGDLKKAQVDANTALDKAAEKERGEINKDATLTTEDKAKQLKEVETALTKAKDNVKA
                     AKTADAINDARDKGVATIDAVHKAGQDLGARKSGQVAKLEEAAKATKDKISADPTLTS
                     KEKEEQSKAVDAELKKAIEAVNAADTADKVDDALGEGVTDIKNQHKSGDSIDARREAH
                     GKELDRVAQETKGAIEKDPTLTTEEKAKQVKDVDAAKERGMAKLNEAKDADALDKAYG
                     EGVTDIKNQHKSGDPVDARRGLHNKSIDEVAQATKDAITADTTLTEAEKETQRGNVDK
                     EATKAKEELAKAKDADALDKAYGDGVTSIKNQHKSGKGLDVRKDEHKKALEAVAKRVT
                     AEIEADPTLTPEVREQQKAEVQKELELATDKIAEAKDADEADKAYGDGVTAIENAHVI
                     GKGIEARKDLAKKDLAEAAAKTKALIIEDKTLTDDQRKEQLLGVDTEYAKGIENIDAA
                     KDAAGVDKAYSDGVRDILAQYKEGQNLNDRRNAAKEFLLKEADKVTKLINDDPTLTHD
                     QKVDQINKVEQAKLDAIKSVDDAQTADAINDALGKGIENINNQYQHGDGVDVRKATAK
                     GDLEKEAAKVKALIAKDPTLTQADKDKQTAAVDAAKNTAIAAVDKATTAEGINQELGK
                     GITAINKAYRPGEGVKARKEAAKADLEREAAKVREAIANDPTLTKADKAKQTEAVAKA
                     LKAAIAAVDKATTAEGINQELGKGITAINKAYRPGEGVEAHKEAAKANLEKVAKETKA
                     LISGDRYLSETEKAVQKQAVEQALAKALGQVEAAKTVEAVKLAENLGTVAIRSAYVAG
                     LAKDTDQATAALNEAKQAAIEALKQAAAETLAKITTDAKLTEAQKAEQSENVSLALKT
                     AIATVRSAQSIASVKEAKDKGITAIRAAYVPNKAVAKSSSANHLPKSGDANSIVLVGL
                     GVMSLLLGMVLYSKKKESKD"
     gene            complement(101664..101780)
                     /locus_tag="ACGEUI_000090"
                     /pseudo
     CDS             complement(101664..101780)
                     /locus_tag="ACGEUI_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        31674 a        18085 c        21640 g        30644 t
ORIGIN      
        1 agatggaagt atggaataca gattttcatc tgtagcttta aaaaataaaa ttaaaagcat
       61 acttgatagt tgatgtatac tgtcatttag aataataatc acatattgtt gtcaacgaaa
      121 cccaccatag tctataatga aagcctagta gggatgagct atataccaac tatctatcaa
      181 tccagacgtc tctactaaaa aatactccgt cttctatgaa actagaaacg gagtatttct
      241 gtttgtctat accactaaat gaacagttat atagaaaggt gattggatgt ttggaataaa
      301 catcctgaaa aaaatttgag accttcttat atcctaaact agaacgaatg tctagtgaaa
      361 taaaaaaagc gacaccatta ggatgtattt tggactaatg tcaaataagt agacagaaaa
      421 ccgtgttatt ttattacgtt aaactaattt tcttctttct gattaggggt tagtcctaga
      481 ttagccgtat gtgggttgta attgttataa aaattctcaa tgtattcaaa gcagtctaat
      541 tgaacctgtt tgatactttt tttggttgat ttgtctatgc tttaaatact tgaaaaatgc
      601 ttcagttacg gcattatcat aaggatatcc aggtttagaa aaagaatgca tgatagtgtt
      661 gtcatcaatt atttttctca attccctggc cttaaattgt gacccttggt cggaatgaaa
      721 gagaagtgtt ccttcaatct ttcttttatt aagagctaat tctagagtgt cacatgctaa
      781 ctttgcatcc atacggtgac tcagtttcca agcaatacat tttctagaat agagatcaag
      841 tattgcgcag agatagacat atttcttata tccaatagaa atataggtga agtcagtaga
      901 ccatacttga ttaggtttgt caggattaaa tttctggttg agtaagtttt gaggatacgt
      961 tttttgagtc cttttaagag ctgtttttgg cttaacggta gccattttag ggagcgccat
     1021 attcttcatt agacggtaaa ttcttccttc agagattttt gtgtcgtagt ctctttgaag
     1081 aatgactttg atagacttca caccaagtct tttgtttgct ttggtataaa tctcaagtat
     1141 ttgttttcta taaatttgat tatccaattc tctttttgag ggcttatgtt ttagaaattt
     1201 atagtaagtg gagcgattga cacgtaaaac acgatacaag gttgttgtga cgtgttcaaa
     1261 gcgtaaccga taaacagcga ggagtcttac tttaagtttt gcatgaatat ggcacttgct
     1321 ttttttagga taaggttctc ctcttcaagc tgcgcattac gtttttgtag ctcttgtatt
     1381 tgcttggcag taagtacaga attatcttcg agtttgactt gggaatactg tttgatccat
     1441 tttgcaagcg cagaagcgga taccccatag tctttacaga gttcagaccg agtttttcca
     1501 gtttgataaa ggttaacaag agattgtttg aattcttcat catagcgttt aactgttgac
     1561 ataggctgtc ctttcgtttt tgtgtctttt aaaacagatt gtaacacaca aagttttgtc
     1621 tacttttata gtatacatcc agtggtcatt tataaaaata aaaaaagtag cagttcactt
     1681 caaaaacggt caggttatcg aaatataatc tggctgtttt ttacgaatgg aatgaaagct
     1741 gctttttatg atataataag aaaaattgaa cagaatgcag aaatgatggg gcttatttaa
     1801 atcgttaaaa agtgcacacc ctaaatggcg tttgcacccc cctagagtaa aaaaaggaga
     1861 cattgtatga taaaactaat agcaacagat atggatggta ctttcctagc tgaagatggt
     1921 acttataatc aagagcaact agctgcgctc ttgccaaagt tagccgaaaa aggcatttta
     1981 tttgcggttt cgagtggccg ctcgctctta gccattgatc agctctttga gccctttttg
     2041 gatcagattg ctgttattgc agaaaatggt tccgtggttc aataccgtgg tgagattcta
     2101 tttgcagata tgatgaccaa agagcagtat accgaagttg caaaaaagat tttagccaat
     2161 cctcactatg tagaaacagg tatggttttt tctgggcaaa aggcagccta tattttaaaa
     2221 ggcgcatcag aagagtacat tcaaaaaaca aaacattact atgctaatgt taaggttatt
     2281 aatggttttg aagatatgga gaatgacgcc atttttaaag tttctaccaa cttcacaggc
     2341 cataccgtct tagaaggaag cgattggttg aatcaagccc tcccttatgc cactgcagtg
     2401 accaccggct ttgattccat tgatattatc ttaaaagagg tgaataaggg ctttggtatg
     2461 gagcatcttt gtcaagcctt gggcattaag aaagcagaaa cgattgcttt tggggataat
     2521 tttaatgatt accagatgtt ggagtttgct ggtagggcta ttgctacgga aaatgcaaga
     2581 cctgaaatca aagtgatttc agaccaagtc attggccact gtaatgacgg agcagtacta
     2641 acgtatttaa aaggactagt gtaactcatg aatattagaa ttttggcgct tgatttagac
     2701 ggtaccctct acaacactga aaaaattgtc acagatgcca ataaaaaagc cttagcagcg
     2761 gctagagaaa aaggggttaa ggtggttatt accacaggtc gccctctaaa ggccatcggt
     2821 aacttgctag aagagttgga cttgcttgac cacgatgact acagtattac ctttaatggg
     2881 gggttggttc agcgaaatac aggggaggtt ttggataaga gttctctcag ttttgaccag
     2941 gtttgccaga ttcagcaagc tttagaggcg gttggcttac caacagatat tatcagtggt
     3001 ggggatgtgt atagcattcc aagtaaggac ggtaggcatt cgcagtacca tctagctaac
     3061 ccactgttga cctttattga ggtgacatct gtagcggaac taccaaaaga cattacctat
     3121 aacaaaattg tgacagtcac cgatccagac tttttggatc agcagattat aaagctatcc
     3181 ccaagcttat ttgaggattt tgaagccttt aaatcacgag atattatttt tgaaatcatg
     3241 ccaaaaggca ttgataaagc ttttggatta aacttgcttt gtcagcacct tggccttgac
     3301 gctagacatg tcatggctat gggagacgaa gctaacgact ttgcgatgtt agaatgggca
     3361 ggacttggag tagctatggc taatggtgtt tcaggagcta aagccgatgc cgatgccgtg
     3421 accaccttga ccaatgatga gtctggagtt gcagaagctg tgaagacatt tattttagaa
     3481 gaggagagtt aatgggatta tttgatcgtt tatttgggaa aaaagaaacc ccgaaggtag
     3541 ctgaggaaaa gttagaagaa aatcttttga cagaaacaac tcagaaagag gagctttctg
     3601 aaaaagccaa tgagcaagac aagattgagg cggttcaaca agaggacgtt tcttcagagg
     3661 gcgcaggttc ggtggaaaat ggtcccgaag cagcttcagt aaacgcactg gttgaggaag
     3721 aaacaggtga caatagtaat catccatcag aagataccaa tgagtttgct gccgacaaaa
     3781 cagaccttaa ggtaagtgaa cttagtcaat ctacggcatc agaaccaaaa gaccttgttg
     3841 accagccagt tgtggaacag tttccaacaa aacaagccca agctgatgcc tcaaatgatt
     3901 cggcgaatga agaagcagta gacacttcaa aagagcaatc cagtagccaa caggttatgg
     3961 aagactacta ccgtcgtaag gctgctcttg aaaaaagcct tcaagaaaaa gcagcggcaa
     4021 cggttccagt gatgccagaa gaagttccac aagaaaatca ggcctctacc tcagcagaag
     4081 cttctcaaaa caaggcaacc cacgatacca ttcctgagac agatcaagaa aaatacaagc
     4141 gtagcttgaa aaaaacgcgg actggcttct cagctcgtct gaacagtttc tttgccaatt
     4201 tcaggcgagt ggatgaagaa ttctttgaag acttggaaga aatgctgatc ttatcagatg
     4261 tcggtgtaca tgtggcgaca actttgacag aggagttgcg ttatgaagcc aaactggaaa
     4321 atgcgaaaaa accagatgcc ttgaaacgtg tgattgtgga aaaattggta gatatctatg
     4381 aaaaagatgg gcgttataat gaagccatta attatcaaga tggcttgacg gttatgcttt
     4441 ttgtaggtgt taacggggtt ggtaaaacga cttcaatcgg gaagctggct taccgttaca
     4501 aacaagaagg taaaaaagtc atgttggtgg cagcagatac cttccgagca ggggctgtgg
     4561 ctcagctagt cgaatgggga cgccgagtag atgtgccagt cattactggc cctgaaaagg
     4621 ctgatccagc gtctgttgtt tttgacggga tggaaaaagc tgtggcaaaa ggtgtagata
     4681 ttctcctgat tgacacggct ggtcgcctgc aaaacaaaga aaatctcatg gcagagctgg
     4741 aaaaaatggg tcgtatcatt aagcgcgtgc taccagatgc ccctcatgaa acgcttttgg
     4801 ccttagatgc gtcaacagga caaaatgcgc ttagccaagc caaggaattt tcaaaaataa
     4861 caccattaac aggtttgatt ttaacgaaaa ttgatggcac ggccaaaggt ggtgtagtcc
     4921 tagccatccg tcaagaatta gacattccag tcaagtttat cggatttggt gaaaaagtgg
     4981 atgatattgg agaattccac tcagaagact ttatgaaagg ccttttggaa ggtatcttat
     5041 aacagcaaaa accagtctcg ttttattgaa cgagactggt ttttagtatg tcatcttagg
     5101 ctaatccttt tttccctaaa tggtaaaaca tagcaaagaa aaagataaaa tcaagtagag
     5161 caaaaatagt aaagagaagg tagacagact ggacaccgct tgtgaattct gcttgactat
     5221 ttgctatcag tgatgccata gcagttccta gagcgccagc aaattgctgc atcatctgaa
     5281 aaatggccgt ggcatcggca tttttctcgg caggcaattc tcgaatagcc gtggctaagc
     5341 tattattaaa gcccatgtta cgcccaaacg tgaataaaat atataaaaga gtgaatggta
     5401 aaagcataaa atgtcttgtt tgaagtgtca taataatcaa tgataaacta aataaggcat
     5461 ttcctaaata aagcgaaagt cttgctcctt tttgatcata aagtttacca aaagcaggtg
     5521 ctagtagagc tccgagtaag gtaccaggta gtaacaccat accagcttga gaactattag
     5581 caatcttttc catgacaata aagtttggcg ttagaaaatt tatgcctaaa ttaatcagct
     5641 ggaagacaaa aaagggaatc aggccaaagg ttagagaggg aattttgaga atgcgaatat
     5701 caagaaaggg ttgcttagct gtgagattct tgtagaggaa gaggataaag ctgagaataa
     5761 aaagccctaa gtaatacaaa ttaacactgc cgttttctag actagtaata gctaataagg
     5821 cagaagttaa gctaatcgat agtgcaataa atgccaacca gtcaaagggt actttttcgc
     5881 ttactggaga atcttcgagg taataataag ctagaatacc tgcaatcagt ggtacaggga
     5941 gtatacagat aaaaatccat tgccaactaa aatggctaat cataaagccc ccataagtag
     6001 gcccaaaagc aggtgctaag ctaataataa gaccagcaaa tcccataaat agacctacct
     6061 tatgcattgg gacacgctct aaaataatat taaacatctg aggcattacc agaccagtac
     6121 caatgccttg aaaaatgcgg gctagcaaca tgatcgcaaa ggactgggtc agaacggcca
     6181 agatggtgcc aaatgtaaaa agacctgtgg ccataaaaaa gaggggtctt tcccgcacat
     6241 tttttttcag tgtagccgaa gtggtcatca taatcgccac tgctagtaaa taaatggtcg
     6301 tcatccattg caagctattt aagggtaact gatagactga catcagtgtc gggaaggtga
     6361 cattcatact ggtttcagac aaaattcctg aaaaaccaat gagtgctatg gcgataatcg
     6421 ccataattgt ttgttttgaa acggtttgtt gatgatgtga catgataact cctttatcta
     6481 atcgattgtt attgtatcat ataatcttaa aattgtcaga aactcttttt tgctaaatca
     6541 atagatacga ataacaatag ttgctctttc agagaatttt ttgaaaacgc ttgacagaat
     6601 cttaaagaga tgataacatt aaagcgttct cagatgtgat tacttcaggt aagcaggcta
     6661 gagacataac aatcagaata ttaaaggatg ttatcacagt tttgtgagca gggacctatt
     6721 tgaaaagtgc tttttagcga gcttttggat aggtcatttt ttattgtgat actaaaggac
     6781 taggactttt atgttgatta aacgcgtttt aaaccataat gcggcgattt cgaccaacca
     6841 tcaaggatta gatattttat tgatgggaaa agggattgcc tttggaaaaa aagttggaga
     6901 tagtattgag cttaatgcta tcgaaacatc atttgtttta aaaaatagtg acaatatgaa
     6961 tcgcttcaca gagttattta ttacggtgcc acaagaagtt gttgcttgct cagagagaat
     7021 tatcaatcta ggtaaaatta aattaggcaa aaccttagat gaaattctct atattaattt
     7081 aacggatcat attcactctg ctattgaaag gcatgagcaa ggaatgttga ttcataaccc
     7141 actacggtgg gaaattcaac gctattatcc tgatgaatat agtctagggg taaaagcctt
     7201 agagctaatt gagcgtaatc taggagtcac tctagctatt gatgaagcag cttttattgc
     7261 catgcatttt gtcaatgcta gtttggacac tccttttaag gagccacatc gcctgacgga
     7321 gattgtttct tatattgagc aaaaaatcaa aacagacttc aaaacagaat tggatgacac
     7381 atccatcgat tattaccgct ttatgaccca tattaaatta tttgctcaac gtgtgttatc
     7441 acagatgtct tacgatgatg atgatgcgga actgctttta gtggtgaaaa cgaaataccc
     7501 taaagaatat cggtgcgtct tagatattag tgaggagata aaaaagcggt acaactacca
     7561 ccttaattca agcgaacttt tgtatttgac ggttcatgtt aaacgtttag tgaaacattt
     7621 aaaggagact taatatgacg tatcaagaaa ctgccaaggc tattttagcg gcggttggtg
     7681 gcaaaacaaa tattcaacgt gtgacacact gtgtaacacg tctacgctta gtactgaaaa
     7741 atgatgagaa agttaaagat cagcaagtca aagccatttc aaatgtgatt ggcgtgatgc
     7801 gcaaaaacgg ccagtaccaa attatcttgg gcaatgatgt caataattat taccaagcct
     7861 ttttaagcct agggcatttt gacaatcaag atgaagatca ttcttcaaaa gcgaaaggaa
     7921 gtatccttga gcgcttgatt gaaaccattg ctggcgtgat tacaccgctg attccagccc
     7981 ttcttggtgg tggaatgctc aaagtggtgg gaatcttgct tcctatgctt ggcttagcga
     8041 gtgcggactc tcaaaccgtt gcctttatta acttctttgg cgatgcggct tattatttta
     8101 tgcctgtcat gattgcctat tcagcagcgg cgcgctttaa ggtgacacct gttttagcag
     8161 cgacgatcgc agggatttta ttgcatcctg cctttgttgc aatggtagca gaaggtaaac
     8221 cattgacttt atttggagca ccagtcactc ctgctagcta tggctcatct gttattccca
     8281 ttttgatgat ggtttacttg atgcaataca ttgaaaaatg ggtcaatcgc ttggtgccaa
     8341 gcgtgatgaa aagcttctta cagccaacct taatcatttt gatttctggc tttttggctt
     8401 tagtggttgt aggtcctctt ggtgtgatta ttggtcaagg gttatctaat accatgctcg
     8461 ctatttatca tgtggctccg tggctagcac ttgctatttt gggagcgatt atgccgcttg
     8521 ttgtcatgac ggggatgcac tgggcttttg caccaatctt tttggccgct tcggtcgcaa
     8581 caccagatgt cttgatttta ccagcaatgt tggcttctaa tttggctcaa ggagccgcat
     8641 ctttggctgt tgcctttaaa acaaagcaga aacaaacacg tcaagttgcc cttgccgcgg
     8701 ggatttcagc tttgctggca ggtatcacgg aaccggcact ttatggggtc acactgaaat
     8761 ttaaaaagcc actctatgca gccatgattt caggtggtct ggttggagcc tttattggtt
     8821 ttgtcaatat tgcttcttat acctttgtcg taccttctat tattggttta ccacagtaca
     8881 tcaacccatc aggcggagct aattttacaa atgctttgat tgccggaact gcgacgattg
     8941 tgttagcctt cagtttgact tggttcatgg gaattgatga agagtcccca aagcaagtga
     9001 gtgttgcagc agatatgtca caagttaaga gcggcttgtc aaccaaacaa acgttgtatg
     9061 ctccaatgac aggtgagatg ctttttctat cagaagttcc tgatgaaacc ttttcttcca
     9121 agttattagg agaaggattt gccatattac caagcgaagg tgaggtctat gccccctttg
     9181 atggtgaagt catcactttc tttccaacca agcatgctgt tgccttaaaa aacacacgtg
     9241 gtgtggaagt cttgattcat gtcggtattg atacggttga gttaaaaggg caaggtttcg
     9301 agcagttagt gtctgttggc gatgtcgtga aacgaggcca ggcacttcta aaaatggata
     9361 ttgatttcat tacttcaaaa ggctattcac tcatcagtcc tgtggtcgtg accaattcgg
     9421 ctgagcagtt ggagattatt attcaagatg acaaaaaaat ggtgactaaa gaggacgctt
     9481 tgttagtcat tttataacct aaaaggagag ggagaatggg aatttttcca aaagatttct
     9541 tatggggcgg cgcagtagct gccaaccaag tggaaggagc atttgaggca gacgcaaaag
     9601 gtttgtccgt acaagatgtt ctaccaaatg gtggactagg agaatggaca gatagtccta
     9661 ccagcgataa tttaacttta gaggcgattg atttttacca ccgctacaaa gaagacattg
     9721 ctctttttgc cgagatgggc tttaaagtct ttcgaacgtc tattgcttgg tctcgtattt
     9781 ttccaaatgg tgacgatgac cagcccaatg aagcaggttt gcaattttat gatgacttat
     9841 tcgatgaatt gttgaactat ggcatcgaac ccttagtgac cttgtcgcat tatgagacac
     9901 ctctgcattt ggccaaagcc tataatggtt ggacagatag acgtctgatt ggcttttttg
     9961 agcgctttgc tcagacagtc atggaacgct ataaggacaa agtcaaatac tggttgacct
    10021 ttaacgaagt caactcgatt ttacacatgc cctttaccag tggtggcatc atgacagaaa
    10081 aagaaaagct gagcctgcaa gatttatacc aagctattca tcatgaattg gtagcatctg
    10141 ctagtgtcac aaagctagcg catgaaatca atcctgatgt taaagttggg tgtatgattt
    10201 tggcgatgcc tgcttaccca atgacctctg atcctcgtga tatacttgct gcgcatgcat
    10261 ttgaaaatct taatctctta ttttcagata ttcatgtgag aggcaagtat ccttcatata
    10321 ttaagtcgta ttttaaagaa aatgggattg agattgtttt tgaagacgga gacaaggaat
    10381 tattagcgga acatacggtt gattttctat cctttagcta ttacatgagt gtcacacaag
    10441 cccacaatcc tgaagcttac acatctggtc agggaaatat tctaggtggt ttgtccaatc
    10501 catatttaga atcttcggag tggggttggc agatagaccc aattggtctg cgattggtct
    10561 tgaatcagta ctatgaccgc taccagattc ctttgtttat tgttgaaaat ggcttaggtg
    10621 caaaggatca gctggtgcaa accgcagatg gcagcatgac ggttcacgat gattatcgca
    10681 ttgactacat gagtcagcat ctcgtacaag ttgccaaagc gattgaagat ggcgttgagg
    10741 tcatgggtta cacttcttgg ggctgtattg attgtgtctc tatgtcaaca gcccaattaa
    10801 gtaaacgcta cggttttatt tatgttgacc gaaatgatga tggtactggt caactcacac
    10861 gctataagaa aaaatcattt gattggtacc gacaagtcat tcaaaccaat ggacgttacc
    10921 ttgaggataa tttttcctaa ataggtcttg tttaagatgg tgactctgtt cctatgctaa
    10981 ctgataaaac tcaaaaagca tttgttttga taaaaaacaa gtgctttttt attagtcttt
    11041 aaaaaacgcg gtaaacataa ggattattag gttctttggt ttgatgaacc tcagcaagtt
    11101 gaaggacagg tagatgttgt tctaagtttt tatcatattc catatgaagg cgacctttaa
    11161 ctgttagcca tgtgttgtta gggtagctgg tttgattacc tgtggtcaaa agtccgtaca
    11221 ctcctgagtc ggcaatacag tgaataatgc cgaagcggaa aaggaattgg tagttgtcat
    11281 ggcccggttc gttataaaca aagccagtgt attggatgtc acgatcaaga aactcatctg
    11341 ggtagagata gatcaattcc atcacttcca tataattttc agtggtaatg gtgaccggtt
    11401 tcttgccttt atatttatgg agttcttgac gcatctcctt ttggtaggca gactttgtaa
    11461 agtaaaggct ggtatcaggt ttgaggtatt gaatggtggt accgtcatca ctgactcccg
    11521 ttttagaggc tcccgcagcc aatgggaaag tatagccttt agcagaaacc gtggtggaat
    11581 ctaaggtcac ggtcggcaca aggagaccaa tcaaaacagg aaagaccaag ataaaaggac
    11641 tcgtcagcct tgcgattttt cctgttaaat ggctatgtac cttaatgttt ttcatccagg
    11701 tatagagttg aacaagagct aaaatgaaag ataaaatcat ggaaatataa gcaaggtaag
    11761 aataccgaac attgatgtac tgatccagtt tgccagacag ctggagatac atggttagtt
    11821 caaaatagcc agctaaaatt aaaaagcgaa tcattagagc acccccacaa gtaaacaata
    11881 aacggcaatc ataagaacgg atacgccgat aaattggaca atgaagcgtc ctttaaaagc
    11941 ctttaccatc atcataaggt ttttaatatc caccatagga ccaattaaaa gaaaggcaag
    12001 aactggagcc actccaaagg tggataataa ggaagctccg ataaaggcat cggcttcact
    12061 acataatgat agaatgaaag ccatcagcat catgatcaaa atggctgtca agggattgtg
    12121 cccaatcgtt gtcaacacac gagttgggac atatatttgc atggcagagg caatcaaggt
    12181 tccaaatacg agatacctac cggtgtcgaa gaattcatca atagcatgta ccagtgctag
    12241 ataaatgcgt tttggcaagc tttcatgaga ataatcatga aaatgcacgg gttgagcatt
    12301 atccttgaga atgttatcat ctacaataaa agctaacatt accccaagtg taatggctac
    12361 tagtgcagca ccgaccaagc gaagtatcag aaagcgcaaa gaattcccaa aagcagaata
    12421 agtagcgaac aaaacaatag gattaatgat aggcgctgtt gccagaaaag ggacggccgt
    12481 ataactaggc accttttttt ccaaaaagcg gttgatgatg ggaataatcc cgcattcaca
    12541 agaaggaaaa acaaatccaa caaaggttcc aaacagaatg cgcaagcatt tttgcttagg
    12601 taggtatttt tgaaccaatt ctggcgttac aaagacttca atacaaccag ataaaatagt
    12661 tcctaggagc acaaaaggaa gagcttcaat gataatggac ataaaaatcg caaaccattg
    12721 caagacactg ggaggaaggt tggaaaagag tgacattcac catatccttt ctagatgata
    12781 aaagaggagg ctgaaacacg aatgttcagc ctcgactgat acgttttgtt agcctgatcg
    12841 ctaaaaatct ctcaactcag tttaaacgaa tggacgatat gttaagagaa tgagacaaac
    12901 tagctatggt ttgcttggtt ttgtttgttc tgtcgttttt tctttggtcg ctagtttacc
    12961 atcagtattg acttccaaag gcttatcaaa actagggatt gatgcaaagc ctgctagcat
    13021 tttttcaagt tcatcatttt ttttatgggt aattgccata tctatgacct tctttcattc
    13081 aagataagtc tattatacta tcatttgaga acaatgccaa agcggaagtg ttgtttactt
    13141 ctttcttttt taaaattagg tattgttatt tagggtttcg tcagttattt actaaaagta
    13201 gctttttttg attctttacg caactttata ttaggacaat gtttcgctat cattttttgc
    13261 attaacatgg tttgtgttat aatagcaata ttatggaaaa taacaataat cacaacattg
    13321 cagaggcttt gtcagttagt ctccatcaaa ttgaacaggt gcttgcccta acagcacaag
    13381 gcaataccat tccctttatt gcacgttacc gtaaagaagt aacaggaaat ctagatgaag
    13441 tggtgattaa gtccattatt gatatggata agtctctcac taccctgaat gagcgcaaag
    13501 caaccattct cgctaaaatt gaggaacagg gaaaactgac agatcagtta cgaacgagta
    13561 ttgaagcaac cgagaaactc gctgatttgg aagagttgta cctgccttat aaggaaaaac
    13621 gccgtacaaa agcgacgatt gcgcgtgaag caggcttgtt cccattagca cgtttgattt
    13681 tacaaaatgc gcaaaacctt gaaacagcag cagaaccctt tgtcacagaa ggatttgcca
    13741 gcccacaaga agctctagca ggagctgtgg acatccttgt ggaggccatg tcagaagatg
    13801 ctaaattacg ctcttggacc tacaatgaga tttggcagta cagccgctta gtatcaacgc
    13861 ttaaagatga gcagttggat gagaaaaagg ttttccaaat ctattacgat ttttctgacc
    13921 aagtgtctaa catgcaagga tatcgtactt tagcccttaa ccgtggcgaa aagttaggca
    13981 tcttaaaagt gtcttttgag cataacttgg agaaaatgca acgctttttc agtgtgcggt
    14041 tcaaagaaac caacccttat attgaagagg tcatcaatca gaccatcaaa aagaaaattg
    14101 ttccagctat ggaaagacgg gttcgttcag aactcagtga tgccgcagaa gatggggcta
    14161 tccacctctt ttctgaaaat ctccgtcatc ttttgttagt gtctccttta aaagggaaaa
    14221 tggttcttgg gtttgaccct gcctttcgaa caggtgcaaa attggctatc gttgatcaga
    14281 ctggaaaatt gttgactact caggttattt atcccgtagc accagctagc caaacgaaga
    14341 ttcaagcagc taaagaaaca ctgactcagc tcattgagac ttaccagatt gatattattg
    14401 ccattggaaa cggaacagcc agtcgggaaa gtgaagcctt tgtagcagac gttttgaaag
    14461 atttcccaaa tacgtcctat gtcatcgtta acgaaagcgg tgcttctgtt tattcagcgt
    14521 cagagttagc ccgccatgag tttccagacc tcacagtgga gaagcgctct gctatttcta
    14581 ttgctcgtcg cttgcaagac ccactcgcag aattggttaa aattgatcct aaatctattg
    14641 gcgtcggtca gtaccagcac gatgtgagtc agaaaaagtt gagtgagaat cttggttttg
    14701 ttgtggatac tgtggttaac caagtcggtg ttaatgtgaa cacggctagt ccatctttat
    14761 tagcacatgt gtcaggtttg aacaaaacga tttcagaaaa tattgtcaaa taccgcgaag
    14821 aaaatggagc actgacatca cgcgctgaca tcaaaaaagt gccgcgttta ggagccaagg
    14881 cgtttgagca agcagcaggt tttctcagaa ttccaggagc caaaaatatt ttagacaaca
    14941 caggagttca ccccgagtcc tacccagcag tcaaagaact ctttaaggta cttggtattc
    15001 aggacttgga cgacgctgcc aaggcaactt tagcagcagt tcaagttccc caaatggcag
    15061 aaacattggc tattgggcag gaaaccctta aagatatcat tgctgatctc cttaaaccgg
    15121 gtcgtgacct tcgtgatgac tttgaagcac caatcttacg tcaagatatc cttgatttga
    15181 aagatttgga aattggccag aagcttgaag gaactgtgag aaacgtcgtt gactttggtg
    15241 cttttgtaga cattggtgtt catgaagatg ggcttattca catttctgaa atgagcaaaa
    15301 cctttgtcaa ccaccctagt caagttgttt cagtgggtga cttggtaact gtttgggtct
    15361 ctaagattga cttggaccgc cacaaggtca atctcagcct attgccaccg cgtgacactc
    15421 actaattatg tccaagaagt ttccctggct gattttggca aatcacttca ccacaaggct
    15481 tattggaaca agcgcctaaa aacaactggc ggtcgctttt ttccaaaaga tggtcatttg
    15541 gattttaacc ctaggatgct tgaggagcac ggtgagctta tttttcggaa aattgttcgc
    15601 catgagctct gccattacca tctttatttt gaaggaagag ggtaccatca taaagatagg
    15661 gatttcaaag atctgctagc tcaagtaaac gggcttcgtt atgtcccaac tagctctaaa
    15721 tcaaagacta atcatcatta tagttgccaa acctgtgggc aggtttatca acgaaaaagg
    15781 cgtatcaatc tggctaaata tgtttgtgga aattgccacg ggaaacttat ggaaaaaaat
    15841 cagtcgtaag actggtttta ttttgcgaaa aacaatgtaa actagtatca tagaatgagg
    15901 aggtaaagaa acgtggaaac taaattctat aaacagcgaa aaaatcgctt ggttgcaggt
    15961 gttattgctg gtttagcaga taagtatggt tgggatcttg ctctagcgcg tgtcttagcg
    16021 gctcttctta tttacggaac tggttttgga gttttgcttt atattttgtt ggccatcttc
    16081 ttaccttaca aggaagactt gcttgaagaa cgttatggcc gaggtcctcg tcgtcgtaaa
    16141 gacgctgatg tcttaaatga agaagaagat ggttggttct ggtaaataat cagttaaaat
    16201 gataattcat cacgtaagag caaccatgtg attaactgat gataataaaa tgttttgata
    16261 tgtaagtaag cgaagggtct tatcaactat gatgaggctt tttgcttttt gacttagtaa
    16321 gatgatacct ttttaaacac cataataagc actaattgct caatgatact agtaaagtat
    16381 ttgtttgcca ttgtcttatt tttgactcat atctctaaat gataaaacct agtttttaat
    16441 agagctaaga ccatttttgg tgtcgttgtt ctcatttagt agtgaaaaaa tgagtatttg
    16501 caagggtcta acggtcgatt tgtgttataa tattgaaaaa ggagaaatta tgaccgttac
    16561 tgtaaagatg ctagtgcaaa aagttaaact tgatgtggtt tatgctacag ataacttgct
    16621 gtcaaaagag ataacaacat cagatatttc acgtccaggt cttgaaatga ctggttattt
    16681 tgattattat gctccagaaa ggttgcagct atttggaatg aaagagtggt cttatcttac
    16741 acagatgact tctcacaacc gttattctgt tttaaaagag atgttcaaaa aggacactcc
    16801 tgcagtggtt gtctctagaa atttagccat tccaaaagaa atggttcaag ctgcaaaaga
    16861 agaaggtatt tcacttttaa gtagccgtgt ttcaactagc cgcttagctg gagaaatgtc
    16921 ttattttctt gacgcttctt tagctgaacg tactagtgtt cacggcgttt taatggatat
    16981 ttatggtatg ggggttctta tccaagggga ttctggaatt ggtaaaagtg agactggttt
    17041 agaattagtt aagcgagggc atcgtttggt tgcagatgac cgtgtggatg tctatgccaa
    17101 agatgaagaa accctttggg gagagccagc agagatcttg cgccacttgc ttgaaattcg
    17161 tggggtaggt attattgatg tgatgtcgct ttatggagct agtgctgtga aggactcatc
    17221 acaagttcaa ttggccattt accttgaaaa ctttgaggct ggtaaagttt ttgatcgtct
    17281 cggaaacggt aatgaggaga taactttctc aggagtgaga atccctcgta ttcgtattcc
    17341 ggtaaaaaca ggtcgaaatg tttcagtggt tattgaagcc gctgcgatga atcatcgggc
    17401 taaagaaatg ggctttgatg ctacaaaaac ttttgaagat cgtttaactc aactaatcac
    17461 caaaaatgag gtgagtcaat gatcaatccc attgccttaa aatgtggtcc actagccatc
    17521 cactggtatg ctctatgtat cttgtcagga cttgttttgg cggtttactt agcatcaaaa
    17581 gaagcaccca aaaaaggaat atcatctgat gctattttcg attttattct gattgctttt
    17641 ccacttgcta ttgttggtgc aagaatttac tatgttattt ttgaatggtc ttattatgtc
    17701 aagcatttag atgaaatcat agccatttgg aatggcggta ttgctattta tggcggtctc
    17761 attacaggag ctcttgtact tttggcttac tgttataaca aggtgctcaa tcccattcat
    17821 tttttagata ttgccgcacc aagtgtcatg gtcgctcaag ctatcggtcg ctggggaaat
    17881 tttatcaacc aagaagccta tggtaaagct gtgagccagt taaattactt accaagcttt
    17941 attcaaaagc aaatgtttat agagggaagt taccgcattc cgacctttct ctatgaatca
    18001 ctttggaatc ttttgggctt tgtcattatt atgatgtggc gtcgtaagcc aaaaagtcta
    18061 ttagatggag aaatctttgc attttactta atttggtatg gtagtggaag actagtcatt
    18121 gaaggtatgc gaacagatag tcttatgttt ttaggtattc gcatctccca atatgtgtct
    18181 gccttattaa ttattattgg cttaatcttt gttataaaaa gacgtcgtca aaaaggaatt
    18241 tcttattatc aagaataagg atagaaaaag gagaaactta tggacttagt tggaatctca
    18301 ctgatgatta ttgcactcgc atttgttgct ttggtgattt tcttgattat tgtattaaag
    18361 aaagtctcag aaacaattga tgaagccaaa aaaacgattt ctgtattgac aagtgatgtt
    18421 aatgtgacac ttcatcaaac taatgatatt ttagcaaaag ctaatatcct tgttgaagat
    18481 gttaatggta aagtagcaac catcgatcca ctgtttgttg ctattgctga tttgtcagaa
    18541 agtctttcag atttaaatag tcaagcaagg cattttgggc aaaaagcaac taatgctaca
    18601 ggtaatgttt caaaagctgg aaaattagca ttagttggta aagtagcctc aaaagtattt
    18661 ggaaaaaaag gagaaaagca tgaataaaag tttcaaaaac ttagtgattg gagctgtttc
    18721 aggagtagcg gcagcttact tcttatcaac agaaaaaggc aaggctttga aaaatcgtgc
    18781 tgaaaaagct tatcaagcct acaaagaaag tccagatgat taccaccaat ttgctaaaga
    18841 aaaaggtagt gaatacagcc atttagcacg tgacactttc tatgatgtga aagataagtt
    18901 ggcttctggt gacttaacta aagaggacat gttagacttg ctcaaagata aaacaacagc
    18961 tttcgtgcaa aaaacaaaag aaacccttgc cgaagttgaa gcgaaagaaa agcaagatga
    19021 tgttatcatt gatttaaatg aagaagacat catcattgac tatactgaac aagatgagcc
    19081 tgttagtgat acactagata aacactaact aaaacgacct aaaacataga agaagaaaag
    19141 attgaaggat ctgatcacta gtcccgatgg ttagttttag gctaactgaa gaagggggga
    19201 ttgtggcttc aatctttttt tgtttttgac acaaaaaaaa gagtcacgta aaacggactc
    19261 tggaagttac atcagtaatg atttgataac cttggcgaat aaggaagtaa tgcctaagct
    19321 gatgatgctt gcaaataaga aagcaaacat tggcgctaaa ttcattacga gcaggacaaa
    19381 tgcaaataaa acggtaataa ggctgaaact agcgatacga gcaaagaaga ctaattcttt
    19441 tagctttgca ctttggccat tgaactcttg aaaatctcga aatttacttt gagattcttg
    19501 gtaagaatca tcctgttcaa ccgaattatg cttctttaac ggtgcaggca tagtcctctc
    19561 ctttacttaa catactaagt aattctatca taaaagtctc ttcttgtcat ctttttttaa
    19621 aagatttgaa aagatttact aatgacaagt gacttatcat gctataacag taaggggact
    19681 agcttaacgt ttgggctttt gcaagaaata tgcttaaggt cttaaaattt ttggtataat
    19741 aaagcttata tggaaaaaat aattattaca gccacagctg aaagcattga gcaagtcaaa
    19801 gctttattag ctgctggtgt tgatcgtatt tatgttggtg aagccaatta cgggttacgt
    19861 cttccccata atttttctta tgatgaattg agacaaattg ccaaactagt gcatgatgca
    19921 ggcaaagagt tgacagtagc ttgcaatgct ttgatgcatc aagatatgat ggatcagatc
    19981 aaaccctttt tagacttaat gatagagatt gctgttgatt atcttgtggt tggtgatgca
    20041 ggtgtgtttt atgtcaataa aagggatggc tataacttta aactgattta tgatacctct
    20101 gtttttgtga cttctagccg tcaggtaaat ttctgggggc aacatggtgc tgttgaaagt
    20161 gttttagcaa gggaaatccc atcagcagag ttattcacct tagctgaaaa tttggaattt
    20221 ccagcagagg tgctagttta cggcgcctcg gtgattcatc actcgaaacg tcccttgcta
    20281 gaaaattatt accattttac aaagattgat gatgaggtta gtcgtgaacg aggacttttc
    20341 ttggcagaac caggagatgc tagcagccat tactccattt atgaagacaa tcatggcact
    20401 catattttca ttaataacga tatcgacatg atgtctaaat tgggtgagct gtatgcgcat
    20461 ggcttaactc attggaagct agatggcatt tactgtccag gtgatgattt tgtcgctatt
    20521 actaaattgt ttattcaggc taaaacctta ttagaagctg ggcaatttac ccaagaagaa
    20581 gctgaaaagc ttgatcaagc agtgcatgcc catcatccag ctggccgtgg attagatact
    20641 ggcttttatg agttcgatcc aaaaaccgtt aaataagtaa tccttgtctt gagtgagtgg
    20701 gcaagacagg gttatgacac cattacacag gtaatctacc tatcttgaaa atagaaagaa
    20761 tatcacatgt cacatatgaa aaaacgtccc gaggtcttat cacctgctgg aacacttgaa
    20821 aaattaaaag ttgcgattga ctatggcgca gatgctgttt ttgttggagg gcaggcctat
    20881 ggcctaagaa gccgcgctgg taacttctct atggaagaat tgcaagaagg cattgattat
    20941 gcacatgcgc gtggagctaa ggtctatgtt gctgctaaca tggttaccca cgaagggaac
    21001 gaaattggtg cgggcgagtg gtttcgtcaa ctgcgtgata tggggcttga tgcggtcatt
    21061 gtttcagatc cagccttgat tgttatttgt tcaacagaag ccccaggttt ggaaattcat
    21121 ttgtcaacgc aagcttcatc taccaattac gagacctttg aattttggaa agccatgggc
    21181 ttgacccgag ttgttttagc tcgcgaggtt aatatggccg agttagcaga aatccgcaag
    21241 cggacagatg tggaaattga agcctttgtc catggagcca tgtgtatctc ttattcaggc
    21301 cgctgtgttt tgtcaaacca catgagtcac cgtgatgcca acaggggcgg ctgctcacag
    21361 tcttgccgct ggaagtatga tttgtatgac atgccatttg gaggagagcg ccgctcctta
    21421 aaaggggaaa ttccagaaga ctattctatg tcctctgttg acatgtgtat gattgaccat
    21481 attcctgacc tgattgaaaa tggggttgat agcttaaaaa ttgaaggccg aatgaaatct
    21541 atccactacg tctcaaccgt aaccaactgt tacaaggcgg ctgtagatgc ttacatggaa
    21601 agcccagaag ctttttatgc tatcaaagag gaattgattg acgagttgtg gaaggttgcc
    21661 cagcgcgagt tggctacagg tttttactat ggtatcccaa ctgaaaatga acaattattt
    21721 ggtgctcgcc gcaaaattcc acaatataaa tttgtcggag aagtagttgc ctttgactca
    21781 gctagcatga cagcgagcat tcgtcagcgt aatgtcatca tggaaggcga tcggattgaa
    21841 tgttatggac caggtttccg tcattttgaa acggttgtta aggacttaca tgatgcggat
    21901 ggccaaaaga ttgaccgtgc cccaaatcca atggaactct taaccatctc tttaccgaga
    21961 gaagttaagc caggggatat gattagggct tgcaaggaag gtctggttaa cctctatcaa
    22021 aaagatggca ccagtaaaac tgttagaaca tagtaacaag aaatactaga ggccctcttt
    22081 ttccaaatgg tatacgacca tagttgggaa aagagagcct gttttttttg aaaattggat
    22141 atcacaatag cttattttct caaaacttga tagaataata ggagaatgag tcataaaaaa
    22201 agtgaaaaaa agatgatttt ttccacttta atttcgaata gtatagatga ggaggaaaac
    22261 taatggcaga atttacattc aacattgagg aacatttact caccttatca gaaaatgata
    22321 aaggttggac gaaagaattg aaccgcgtca gctttaatgg cgcagaagcg aaatgggata
    22381 tccgcacgtg gagtccagac catacgaaaa tggggaaagg cattactctt actaatgaag
    22441 aatttaaaac catcttagac gcttttcgta agtaatgtta ccttaatcga aacataatcc
    22501 ctcgttcgcc cctgtccttt taacctggtg atgacatcgc taggttaaaa ggacaggggc
    22561 gaatagaagg attatttttt tgaattgagg cgatcagcaa ggccgacaag taccaaaatt
    22621 aagcctagcc caagaaaaat ccatttggca gctaaaaggc cataaagcaa gaagatagca
    22681 gcactaatgt aggagatata ttctttggtc atcttgttaa gtcactttct ataagctact
    22741 gttaataaac cagtcttcat ggtaatgaag actggttaga gataataaaa cgttatgtta
    22801 tattgccact tatcggtttt aagatatgaa tacaaatctt aaaaaagtta aggtttcatc
    22861 gttgggaata aaagcacatc tctgattgtt gttgtgtttg ttaataacat gcaaagacga
    22921 tcaatcccga ttccgagtcc accggttggc ggcataccat attcaagagc ttcaacgaag
    22981 tcgtagtcaa tgccagttgc ttcgtcatca ccaagttctt tagcttgtgc ttgtgcttca
    23041 aaacgagata attggtcaat gggatcattc aactctgtga aagcattggc gtattctttg
    23101 gtcatgatga aaagttcgaa gcggtcggtg aagcgggtat cttctggatt cttcttagcc
    23161 aaaggcgata cttcaacagg atgaccaaac acaaaggttg gttggaccaa ggtttcttca
    23221 acaaattcct cgaagaaagc gttgatgata tgtccgacac ttatgaagtg tttttcgagt
    23281 gggacctgtt tttctttggc aagtgcaata gcttcttcga cagtcatttc aggccagaaa
    23341 tcagcacctg tcacttcctt gatagcatct accatgtgaa cacgtttgaa aggttcattg
    23401 atacggattt cagttccttg ataatcaata gggccatcac ctctaacagc tttagcagca
    23461 tgttggataa tgccttctgt caggttcatg atatcaagat agtctgcata ggcttggtaa
    23521 acctcaatag aagtgaactc aggattatga gtagcatcca ttccctcgtt acggaagata
    23581 cgaccaattt catagacacg ttccatacca ccaacaatca aacgtttcaa atgaagttct
    23641 gttgcgatac gaagcaccat atcaatgttt tgagcattat ggtgagtaac gaaaggacga
    23701 gcagcagcac caccagcctc gttatgaagc acaggcgttt ctacttctag gaaatcaaga
    23761 ccgtctaaat aacgacggat ttctgaaatc attttggaac gggtcacgaa gcggtcaaag
    23821 ctttcacgat ttgaaattaa atcaaggtga cgcttacggt aaatggtttc aatatcagtt
    23881 aatccgtgga atttttcagg aagaggacga agtgatttag aaaggtgagt caacttggtt
    23941 gccttgattg acaactcgcc catgtctgta cgcatgactt cgccttcaac gccgataaag
    24001 tcgcctaaat cggctttttt gaagatttca tagttgtctt cgccaacaga gtccttgcga
    24061 acataaagtt ggatttggcc ttcacggtct tgaagatgag caaaaccaac cttacctttg
    24121 ccacgttttg tcatcaagcg gccagcaaca atagctgttt cgtttaactc atgtaattct
    24181 tcttttgttt tatcagcata cttttctttt aattcagcag aattggctgt gcggtcaaag
    24241 cgtttaccga aaggatcaat tccttgttca gccagcgccg tcattttttc acgacggaca
    24301 atctgttggt catttaattc ttcgatgtgt tgattagaca tattttcctc caatacgatt
    24361 tcctttccat tgtatcataa aaggggctag aatacaccta tatttcgttg gaaaatgcga
    24421 ctgcagcggg gcattttttt gaaaatgaaa ttgaaaagcc taatggaaaa tagtataatg
    24481 attgggatat taaactagaa aagaaaagag actcatgatt actgctattg tttttgatgt
    24541 ggatgacaca atttacgacc agcaagcgcc ttatcgaatc gccatggaaa agtgcttccc
    24601 agattttgat atgtcagtaa tgaatcaagc ttatattcgc tttcgtcact attcagatgt
    24661 tggattccca agagtaatgg caggggagtg gaccacagaa tatttccgat tctggcgttg
    24721 caaggaaaca ctgcttgagt ttggctatcg agaaatcgac gaagctgcgg gagtacattt
    24781 tcaagaagtc tatgagcacg aacttgagaa cattaccatg ttagatgaga tgcgcatgac
    24841 ccttgacttt ttaaaatcaa agaatgttcc tatggggatt attaccaatg ggccaacaga
    24901 acatcagctg aaaaaagttc gtaaattagg gctttatgat tatattgatc ctaagcgtgt
    24961 cattgtgagc caagcaacag gctttcaaaa accagaaaaa gaaattttta acctggcagc
    25021 agaacaattt gacatgaacc cacaaaccac actatatgtt ggggactctt atgacaatga
    25081 tatcatgggt gcctttaatg gtggttggca ttccatgtgg tttaatcata gaggtcgtca
    25141 gctaaaacca ggcactaagc ctgtttatga tgtggctatt gataactttg aacagctctt
    25201 tggtgccgtt aaagtactct ttgatttacc tgataataaa tttatttttg atgtcaatga
    25261 caagaaaaat ccaattttac agatgggaat caataatggc ttgatgatgg cagcagagcg
    25321 tttacttgaa agcaacatga gcattgacaa ggtggttatc ttgcttcggt taaccaagca
    25381 acaagaaaaa gttttgcgct taaaatatgc cagatagatg aaaccataat agcccactag
    25441 ataataataa aaaaagctag caaagtgagt gtcttgtcag taagagatca atgtgttagc
    25501 tttttttatt attttctagt ggttttacac ctctagaaaa gatttatcat tccaaattaa
    25561 acagtcataa gttaaataat ctttagtttc aagaatagta agactggcat tatctaagcc
    25621 acctttagcg agtaaaagag caggttcaaa acctaataat gagcgaatag ttgctgttaa
    25681 gttagcgccg tgtcctacaa taagaacgtt ttgataatgt ttatctttaa acgattggat
    25741 aagatggcag acacgttgag tcgtttggta aattgattca gcctcgaact gatctggttt
    25801 gaactgggct agattctctc gaaaagccag catttgttgg ggatatatag ctgccattgt
    25861 tgctatttta gccccttcca atttacctaa tctccactcc cgaagctggt cggtgtggta
    25921 aagttttggc tgttggtcaa aagcatctaa gataatagct gctgttgcca tagctctttg
    25981 aaggtcgcta gtataaacgg catcaaaagc caccttagct aactctttgc caagtaagtg
    26041 aatttcgtct tttgcctctt caagtaatgg cgaatcgccc ccagcgcctt gaaagcgacc
    26101 ttctaaattc caaagggttt tcccatgtct tacaaaatat aatttcattt aagcctcctt
    26161 ttagagataa ctgatgcgag cgctaaaaat aaaaaagtaa ccagaaaaag tttaggcatc
    26221 accaagcaaa aataatacct atagtgaaaa gataaagtaa aaaagcgtag tataagtgta
    26281 taatagctag gttctgatta aagtgtaaag cccatattag atctaagaca aatcgacctg
    26341 aatttgtcgt gatatcaagt ctctacaaaa gaaaaagatt aacaggatca atgttaaagc
    26401 agttgagatc aaaataatac taaacaaaga ctagcttaat caaggcgaaa aacttaaaaa
    26461 agaaaccaga taaagttggc agtagtgttc attttctctt ctttaaatca gcaaagcttg
    26521 cgccaacgaa atcagctaga gcctggctag aaatctttat agaacgccca acttcaccag
    26581 ctgaaacgat tatttgacct ttttccagtg cagtttggtc aataaaaata ggatatgaat
    26641 gtttttgacg aatcccaaca ggattattgg caccgtgaat atagcctgtt gtcttttgca
    26701 agtctttttg aggaaccatg gacacttttt tgttacctga tacttttgcc aattgttttt
    26761 ctgataagtg ttctgttaat ggaataatgc caattagagg acctgtttga tcaccagtca
    26821 gagcaagtgt cttgtaaata tctgatggtt gaagatcatc tggaaagtcg ccttctaaag
    26881 cattaagctt gagtccttga tgagcaatat tagccttatc gaggatttgt tctactaagg
    26941 tttttttgag ttttgttttt ttagccatat caagtcctta cctttatcta aacataaaaa
    27001 aatggtataa caacattatt ataccatttt tttaatgtaa gattcaaatg actaaaaatt
    27061 agtctttggg atcaaataat tgaggatgaa ttggtaaagc aatcattaac gctaaacatg
    27121 ttattaaaaa agttaggaca ccagctgtac cattaacgct aaaagaatac cagacagcgg
    27181 acatgccttt aggggcgtaa ctgccccaga aaatgattcc tgcaaggaag tgccacaagt
    27241 aacgtactgt aacggctagt aaactagcca ttattgcaag aaaaacggca aaaaaggttt
    27301 gttgtcttct taaagtttta atcaaactat tagagaaact accggctaat cccatagagg
    27361 taaaggctaa gatatattca ataaagactt gggacatgct gagatagtaa acttttccaa
    27421 gtacaaagtg aagtagcccc caaatgagac ccgccaacat gccgtacttt aacccacggc
    27481 gcaaagagaa aataaccagt gcaatagcac cataagaagg actaaaccag ccagcaaaat
    27541 ctggaataaa tgaaagggtc atcgccaatg cagcaaaaat agcagcttcg ataaggtatt
    27601 tgacattggt gtttggtgac ataaaaaact ccttttgtgc acaaaaagag tatcgctttc
    27661 caatcattga ttattggtca caatccctac gcttgtacta acaagatcag gtcctcagga
    27721 tttcgcaatt gcgatctcag ccaaagcact cctttgtgaa atattatagt gctttcatta
    27781 tatgatgaga taatctcctt gtcaaggata aggggtttta aattattgac ctagagtttt
    27841 tcctggtaag atgtaaacta taaaaaaaga ttagccatga tgctaacctc ttaattgttg
    27901 gagttgccaa ataacttctc aaacgttttt ttagtgtctt ttgttacggc aatttgattt
    27961 aaatctaaag cattgttaaa accactgaga taaccttgtg aggtgtattg gtgtaaatcg
    28021 taatctaagg ttgtattagg agcagcctca taatagccag aatcactacc ataagtcgga
    28081 atccatacag aatcgaaacc ttttgttgaa atatcttgtt ctgccataaa ataagttcca
    28141 atatagagac caacattttc agcaccaagt tttttaagct cttctcggaa ggctgttacg
    28201 cctttattca tatctttcat agtggcttct tcgacatcaa tccagtagta agttggatta
    28261 tagggagcag cgtttttgta gaaagctcgg gcctcttctt tcatctcttt agtactacga
    28321 ccaagtgcgt aactgtaaac agctacagga acatttcgtt tttgaaattc tttgatatgc
    28381 gttttaaaag atttgtcaat tccagttgta aaagcagcgt tgttatgagc tgtaatttgc
    28441 gatcctccat agacacgtac aattgcgcca gagatgtgac gtgacaatgt gtcataatca
    28501 atttcttcag ggagttgcca gccggaaaca tcaacaatag gatttaaaat aaagtcttcg
    28561 gtttcagaag tgctagtttt tgctttacca ggattactgg ttgcaatagg aatatgactc
    28621 ttagcgtctt caatttcttt ttgtttcgca tgattagctt gtctttttcc gataatcaat
    28681 accattgcca gtaaaataaa gaagactagc acaacaatag gttttatttt tcttctcatt
    28741 aactgttatt gtaccctaat tactaaaaaa aatcaaaata tcctaactgt tttcttatat
    28801 tgtcttgcat tttaaggtat tctctttgag aagttacaaa tgagatcctc tctaactatg
    28861 tcaagtttat gtcactatct cttatagttg gtagctatat tctttacaat attagtttag
    28921 tctttgacta tatcatgaca gtcagcaagt actgttttgt tatttaattc tcatgttatt
    28981 atcatttgat aaataagtga gctccaaact ggtaaacgct ctgtaaatct gtcataatag
    29041 agaaaaaaac gaggaagaag tttatgtcag ctaagcttat gagtcgcatt gctatcatgt
    29101 cagccctatg tgttgtgttg agaatggttt ttagcagcct gcctaacgtg cagcctgtga
    29161 cggcattttt actcagttat cttctttact ttgggttagc cgaggctgta ttagttatga
    29221 tgttgtgctt gtttttatca gcctttttgc tgggatttgg cccttgggtc ttttggcaag
    29281 ttacttgttt tgtgcttgtt cttttattat ggcgttttgt cttgtaccct ttgtctcagc
    29341 aattccccaa atatcagctt ggctgtcagg ctttcttggt cgccttatgt ggcctccttt
    29401 atggggtgtt gatagatact tgttttgcgt atttgtatag tatgccttgg tggagctatg
    29461 ttctagctgg gatgcctttt aatatagcgc atgctttatc aactctcgtc ttttttccag
    29521 ttgtgatgat gttatttagg aggttaatcg gtgaacaaaa gatttaaaac aggatttcta
    29581 gcgcttgttg ctatgctact tgttgcttgc tcgcaaggaa caaaacaaat acagacgact
    29641 ccaagcgttc caaaagcaga ccatcacgtt cgcttagttg tcaaagaaga taccaatact
    29701 gttgacgaaa aagtttcttt tggaaaagga gatacggtgc ttgaggttct caaagataat
    29761 tatgaggtta aagaaaaaga tggttttatc acagcaattg atggtatcga gcaagacacg
    29821 aaagctaaca aatactggct ttttaaggtg aatggaaaga tggcagataa gggtgccgat
    29881 caaattaccg ttaaagatgg tgatagcatt gaattttatc aagaagtttt taattagtaa
    29941 ctataatata aaatagactt ggcacgccaa gtctatttta tattaaccaa gaagtgcttt
    30001 aaggtcttct tcaattagtt taggatctgt ctttgaggaa tagcgtttaa tgacttgacc
    30061 attttggtca atgagaaatt tagtgaaatt ccattcgatg cgtttcccaa ggggaccact
    30121 tttttcttct tttaaccagg tgaacaaagg atcagcgtct ttaccattca ctttgatttt
    30181 tgcaaagcga gggaaagtag tgtgataggt cagactgcaa aagtgattaa tctcctcagc
    30241 atctcctgga gcttgattta aaaattggtt gcaaggaaaa tctaagacct caaatccctt
    30301 gtcatggtat gtgtcatata gtgcttgcaa ggcctggtat tgtggggtta gcccacattt
    30361 ggtagctgta ttcacgacca aaaccacttt ttctttgtag tcaactagtg agatgtcact
    30421 gccgtcctga gccttaaccg taaaatcata gagattaggc atgtgacata tcctcggtgt
    30481 aactgataat ttggtcaacg gtatcactca agaattgaat ggtatctcga agaggctttt
    30541 cagtggcaat gtctgcccca atgagcattg cagtatcaag aggcgtacgg ctgccacctg
    30601 attttaggaa gtcaagccac tccttggcac catttggatt atgtttaagg ttgaggtaac
    30661 cagctgttga aataacaagc ccagcagaat aggtataact atataagccc atgtagtaat
    30721 gtgcttggcg catccaggta agagctgcgt catcgtcaat gtcaacggca tctccccaga
    30781 agtctgttaa gacctctttc atcatcgcat ttaattggtc agctccaaag gtaccgcctt
    30841 cttcaataag ggtgtaaacc ttacgttgga aagccgcttc caataggtga gtgatgaagt
    30901 tatgaaagta agtatctgtt aaacggtgag caagggcaaa acgtttttga cgagggtcat
    30961 caaactgatg ttccaagtaa tcactaagca tcaattcatt aaaggtcgat ggcgcttcga
    31021 cgtaataagt tgacatatgg gtgttgaagt aactttggtg attatctgaa aaaatgaatt
    31081 ggccagagtg accgatttca tgaataaggg tataaacatc agacattctt ccagtccaac
    31141 tcattaagac atacggatga accttgtagg gatcagcggc ataaccacca gaatccttat
    31201 tggcgttagc agcaaagtca acccagcgtt ctgtctgata acgttcgatt tctttcgtgt
    31261 attcttgccc taatggggct agagatttca tcacgaggtc ataggcacca tcaatgctga
    31321 cctctggatt gagatcattg tcaatatcta gcttccagtc agcaaaagtc attttctcaa
    31381 gaccattgac ttgggcgaca tgttttaaga atttctgagc aaccggtcca aattcagtca
    31441 tgatgaggtc aatttggcgg tcgaacaagc tacgatccac ttcctgttca gctaggaggt
    31501 aatcaaagac agaagcgtag cctttcatat ccgctaacaa tttttcagat ttaaccttgg
    31561 ctaagtaagc cgctgcagct gtattttggt gtttacggag accttttgaa aaagagcgga
    31621 aggctttttc acgaatctca gcattttcgt gattttgata gaaattttcg taagaaacaa
    31681 agctattttt ataggtttta ccatccacct caaaatcatc catgtcaaag tcaccagccc
    31741 gcattttggt gtaaatatca tagggcgcat tgataacctc gcgtaagtta gccaaagctt
    31801 tttccacatc aggacttaag aggtgttctt tttgaatttt tgccatgcga atagctgcgc
    31861 tgaaataagg gttcttttct aatgtatcta acacgtcaag gtcggcattg gctaaggcgg
    31921 tgtcaaagaa gctaagggca acgctagctt tagtcatgaa atcatcgcca gcctgagcaa
    31981 tttgagcaaa actttcatct gaaaagtcag tggtttgagg cataaaggca taagtgccaa
    32041 tatgactcat ttgaatataa atatgctcaa tttctattaa ggcctgagtg aaatcgtcca
    32101 ccgacgttaa gcggccttca taattgcgct taaacaggtc aatatcttgt aaggcttttt
    32161 caatagcgag taaaaaatct tggcggtctt tgtagagcgc tgttagatcc caaagttcat
    32221 tttcagggaa ttctgaacgt tttttcaatt ccatagttgt ctccttaaca atatactcaa
    32281 gactagtata acagaatttt agtggaatat ggagactaaa cctatccaaa cattctttaa
    32341 aataaagcgc ttacttttgt taaaaaacaa acgttatgaa catgatcact tttatttttg
    32401 gtaaaaagtg gtatgataga agaaactagc aaagaaggag atggtcattt gccacttaaa
    32461 aagttagaga gtagtaacaa tcaagatatc attgctgaag aagttgctct tttgaaagaa
    32521 atgttggaaa atatcacacg acggatgatt ggagatgatg cgtttacagt tatcgagtct
    32581 attatggtgt tatctgaaaa acaagattac atagaattag aaaaagttgt cgccaatatt
    32641 tcaaatcaag aaatggaagt gatttccagg tatttttcta ttttaccctt gctaatcaat
    32701 atttcagaag atgtcgattt agcctatgaa atcaattatc aaaataatac ggatactgat
    32761 tatttaggaa aattagccct aaccatcaag gatcttgctg gaaaagataa tggtaaagac
    32821 attttggagc aggttaatgt ggttcctgtt ttaacagcac atccgacgca agtacaacgt
    32881 aaaacgattc tggaattgac aactcacatt cataaacttc tgcgcaaata ccgtgatgct
    32941 aaggctggtg tgattaatct tgagaaatgg cgtcaggagc tatatcgtta tattgaaatg
    33001 atcatgcaga cagatattat tcgcgagaaa aagcttcagg tgaaaaacga gataaaaaat
    33061 gtcatgcagt attacgatgg ttctctcatt caagcagtga ccaagttgac aacagagtac
    33121 aaaaacttag cccaaaaaca cggtctagag ttagacaatc ccaaacccat taccatgggg
    33181 atgtggattg gtggggaccg tgatgggaac ccctttgtca cagctgaaac cttgtgcttg
    33241 tcagctacgg ttcaaagtga ggttatcctc aattactaca ttgacgaatt agcagccctt
    33301 taccgaacat tttcactgtc gtcaaccttg gtacaaccca actcggaagt ggaacgttta
    33361 gccagtttgt ctcaggacca atcgatttac cgtggaaatg aaccttatcg cagagctttt
    33421 cattatattc aatcgcgtct taaacaaaca caaattcaac taactaatca gccagcagct
    33481 cgtatgagta gtagtgtggg actaaacaca tctgcttggt cctcaccagc tagtttagaa
    33541 aatcccatct tagcctatga ctctcctgtt gattttaaag ctgatttaaa ggctattgag
    33601 cagtcgctac ttgataatgg caactccgct ttgattgagg gggatttgcg agaagtcatg
    33661 caagctgttg atatttttgg cttcttctta gcaagcattg acatgaggca agactccagt
    33721 gtccaagaag cttgtgtggc agagttatta aaaggagcca atattgttga cgattatagc
    33781 tctctttcag aaactgaaaa atgcgatgtt ctcttacaac aattaatgga ggagccaagg
    33841 actttatcct cagcggcagt tgctaaatca gacctattgg aaaaagaact ggctatctat
    33901 actacggcac gagaattgaa agataagctt ggagaagagg ttattaaaca acatattatt
    33961 tcacatacag aaagtgtctc tgatatgttt gagttggcca tcatgcttaa agaagtggga
    34021 ttggttgacc agcagcgggc tcgtgtgcaa attgttcccc tctttgaaac cattgaggat
    34081 ttagataatg cccgtgacat catggcagcc tatttaagcc atgacattgt caaatcttgg
    34141 attgcaacga atcgtaatta ccaagaaatc atgttaggtt attctgacag taataaagat
    34201 ggtggctact tggcatcggg ttggactctt tataaagctc agaatgaatt aacggctatt
    34261 ggtgaggaac atggagtgaa gatcaccttc ttccatggtc gtggaggaac agttggtcga
    34321 ggaggtggcc cttcttatga tgccattact tcacaacctt ttggttccat caaagatcgg
    34381 attcgtctga cagagcaagg ggaaattatt gaaaacaaat acggcaataa agatgtggct
    34441 tattaccatt tagaaatgtt gatttcagct tctatcaatc gtatggtgac tcaaatgata
    34501 acagatccta atgagattga tagttttcga gaaatcatgg atagcattgt tgctgatagc
    34561 aatatcattt atcgaaaatt ggtttttgac aatccccatt tctatgatta tttctttgag
    34621 gcaagtccga ttaaggaagt ttccagtctc aacattgggt caagaccagc ggcccgtaaa
    34681 acgattacgg aaattacagg cttgcgtgcc attccttggg tcttttcatg gtcacaaaat
    34741 cgcatcatgt ttccaggttg gtatggggta ggttcagcct ttaaacgtta tattgaccga
    34801 gcacaaggta accttgaacg cctccaacat atgtatcaga cgtggccttt tttccattcc
    34861 ttgttatcaa atgttgatat ggtattatct aaatcaaaca tgaatattgc ctttcagtat
    34921 gcacaactag ctgaaaggca ggatgttcga gatgtttttt atgagatttt ggatgagtgg
    34981 caattgacaa aaaatgttat tcttgctatc caagaccatg atgctctatt ggaggataat
    35041 ccttccctta agcatagctt gaaatcacgc ttgccgtatt ttaatgtgtt gaactatatt
    35101 caaattgaat tgattaaacg ttggcgtaat aatcaacttg acgaaaatga tgaaaaattg
    35161 atccatacaa ctatcaatgg catagcgact ggtttacgta attcaggcta atgctcttga
    35221 gtattaattg gtatttatag aagttttcaa gtgactaatc agaattttaa aggttagccc
    35281 ttggagcttc tttttttgag gattcttgac tttttctttt gtttgatata gaaagatgtt
    35341 ataatgctaa atgattatct caagaagtag agggaaaact atgaagattg ataaaaggca
    35401 cctcttaaat tattctatat tattgcctta tttaatttta tcggtgatag gcttaataat
    35461 ggtttactca accacaagtg tcagcttgat tcaagcacat gctaatccat ttaaatcggt
    35521 gattaaccag ggagtttttt ggataataag tctagtggct attacattta tctataaatt
    35581 aaaattaaat tttttaacga atacaagggt attaactgtg gttatgttag gagaggcctt
    35641 tcttttaatc attgcccgct tctttacaac tgctatcaaa ggagcgcatg gttggattgt
    35701 gatcggacca gtcagctttc agcctgctga gtatttaaag atcattatgg tatggtattt
    35761 ggcattgacc tttgctaaga tccaaaaaaa cattagcctt tatgattatc aagcactaac
    35821 tcgtcgaaaa tggtggccaa cacaatggaa cgatttacgg gactggcgtg tttattcttt
    35881 gttgatggta cttttggttg ctgctcagcc tgacttggga aatgcttcca ttattgtatt
    35941 gacagccatt attatgtttt ctatcagtgg cattggttac cgatggtttt cggctatcct
    36001 agtcatgatt accggtctat ccacggtctt tcttgggact attgccgtta ttggggtaga
    36061 aagagttgct aagattcctg tttttggcta tgtcgctaaa cgttttagtg ccttttttaa
    36121 tccttttcat gacttgaccg attcaggtca ccaattagcc aattcttact atgctatgag
    36181 taatggtggg tggtttggcc aagggttagg aaattccatt gaaaagcgtg gctatttgcc
    36241 cgaagcacag acagactttg tcttttcagt tgtcattgaa gagttaggtc taataggagc
    36301 agggtttatt ttggcacttg ttttcttctt aattttacgt attatgaatg ttggtattaa
    36361 agcaaaaaat ccttttaatg ccatgatggc tttaggagta ggcggtatga tgctcatgca
    36421 agtctttgtt aatattggtg gtatctcagg cttaattcca tctactggtg ttaccttccc
    36481 ttttttgtct caaggtggaa atagcttact tgtcttatct gttgcagtgg gttttgtact
    36541 aaatattgat gctagcgaaa agcgagatga tatatttaaa gaagctgaat tatcttatcg
    36601 aaaggacact cgaaaagaaa atagtaaagt tgtaaatatc aagcaatttc aataatattc
    36661 taaaatctcc tttaaggaga ttttagaata tagacaatcc cagtttgatg ctcaaaaaac
    36721 ttgaactgct ctgctgattt cttatttttg cgtgtgacat gagcccaaac agctgctttt
    36781 tgctgtggca aaaataaagc gtggaattcc aaaggaagtc gctgccgtaa gcaccaccta
    36841 agaaaagtat caaaaaagac tttttcatct atttaaaaaa taaagaaatg gcaaaaaaca
    36901 taaaatactt gcattttcat tgtaatttgc tacaatagta aggtaaagtt agactgtatg
    36961 gcctaccgtc tatctataaa atatatttta ttggaggctt ttcctaaaat ggcaaaagaa
    37021 aaatacgatc gtagtaaacc ccacgttaac attggtacaa tcggacacgt tgaccatggt
    37081 aaaactactt taacagctgc aatcacaact gtattggcac gtcgcttgcc ttcatcagtt
    37141 aaccaaccaa aagattacgc ttctatcgat gctgctccag aagaacgcga acgcggaatc
    37201 actatcaaca ctgcacacgt tgagtacgaa actgcaactc gtcactatgc gcacatcgac
    37261 gctccaggac acgcggacta cgttaaaaac atgatcactg gtgccgctca aatggacgga
    37321 gctatccttg tagttgcttc aactgatgga ccaatgccac aaactcgtga gcacatcctt
    37381 ctttcacgtc aggttggtgt taaacacctt atcgtgttca tgaacaaagt tgaccttgtt
    37441 gatgacgaag agttgcttga attagttgag atggaaattc gtgaccttct ttcagaatac
    37501 gatttcccag gtgatgacct tccagttatc caaggttcag ctcttaaagc tcttgaaggc
    37561 gacactaaat ttgaagacat catcatggaa ttgatggata ctgttgattc atacattcca
    37621 gaaccagaac gcgacactga caaaccattg cttcttccag tcgaagacgt attctcaatt
    37681 acaggtcgtg gtacagttgc ttcaggacgt atcgaccgtg gtactgttcg tgtcaacgac
    37741 gaaatcgaaa tcgttggtat caaagaagaa actaaaaaag ctgttgttac tggtgttgaa
    37801 atgttccgta aacaacttga cgaaggtctt gcaggagaca acgtaggtat ccttcttcgt
    37861 ggtgttcaac gtgacgaaat cgaacgtggt caagttattg ctaaaccaag ttcaatcaac
    37921 ccacacacta aattcaaagg tgaagtatat atcctttcta aagacgaagg tggacgtcac
    37981 actccattct tcaacaacta ccgtccacaa ttctacttcc gtacaactga cgtaacaggt
    38041 tcaatcgaac ttccagcagg tacagaaatg gttatgcctg gtgataacgt gacaatcaac
    38101 gttgagttga tccacccaat cgccgtagaa caaggtacta ctttctcaat ccgtgaaggt
    38161 ggacgtactg ttggttcagg tatcgtttca gaaatcgaag cttaatttta attaagttcc
    38221 cagaatagca atttaagtca gacaacttaa gtttgaagaa gcccccaatt cgggggcttt
    38281 ttgctatctt aaactccata aaatgctgat aaataagaat gaaaagcttt cagaaaaagg
    38341 gtttttattt gtgaaaatat tcctaaaata taagcaattc cttttaatcg tcggtcattt
    38401 gtgctaaaat agaagatata aataaaaaaa gaagaggtat gtgatatgtc acgtaaacca
    38461 attattgctg gtaactggaa aatgaacaaa aaccctcaag aagcaaaagc attcgttgag
    38521 gctgtagcaa gcaaattacc ttcaactgac cttgttgatg ttgccgtagc agccccagct
    38581 gttgatttgg taaccactat tgaagctgct aaagattcag ttcttaaagt tgctgcacaa
    38641 aactgctact ttgaaaacac aggggcattc actggtgaaa catcaccaaa agtgcttgct
    38701 gaaatgggag ctgactatgt tgtgattggt cactcagaac gccgtgatta cttccatgaa
    38761 acagatgaag acattaataa aaaagccaaa gcaatctttg ctaacggttt aactccaatt
    38821 gtttgttgtg gtgagtctct tgaaacttat gaagctggta aagccgttga gtttgtaggt
    38881 gcgcaagttt cagcagcact tgcaggtctt tcagcagagc aagtagcttc acttgtttta
    38941 gcttatgagc caatctgggc tatcggtact ggtaaatcag ctactcaaga tgatgctcaa
    39001 aacatgtgta aagctgttcg tgatgtggta gcagcagact ttgggcaaga agttgctgat
    39061 aaagttcgcg ttcaatacgg tggttctgta aaacctgaaa acgttaaaga ttacatggct
    39121 tgtccagatg ttgacggtgc ccttgtaggt ggtgcatcac ttgaagctga tagcttcctt
    39181 gctttgcttg atttccttaa ctaagtcatc taaaaaagaa aaggttaaat ccttttcttt
    39241 tttagatgac ttaactctta ctataagaga ataaaggtga ttctacaggc aaatgcatta
    39301 gcctaatagc ggtttaatag cttttttgcc aatctaatga aatgatattt gattggaaat
    39361 ggataacatc taaatgtccc tggtttttgc aaaataaagc cattgaaatt ctgtttaaag
    39421 cgcaagacac cgtctgaacc atcaaacttt ccagtaatac caagaaaatt atattgtttg
    39481 atgcccttgt gaattgcctt gagcatagca tgctcttgta atagagctgg gctataaaat
    39541 ttattaaatt tggggtaaga tcctgaaaag aggtagacgg cctcttgttc ggtatagata
    39601 aaaagacttc ccgcaaggaa cacatcttta gttccatact cttcaagaaa atggagagct
    39661 tcagaaattc ttacctgaaa ggtttcgaac tggctggata gttcttttaa tctattttgc
    39721 tttttttcag aatgaggatt tttgccaagg tctcctttaa ctttgttgat gctggttgct
    39781 agttgtagtt gtctttgttt aaggttatta aggtaatctt caaaattaag agtcgcaacc
    39841 ataaattcac aagagtctcc aaaactatca taaaagtcct gatagtaaga taatgatttg
    39901 tcaaggtaat ctctacgatc agaggttgct tcagtaattt ctttgaaatg gtgtaactca
    39961 tctcgtttca gttgacgtaa tttaatacca aaagtattag ctttttttat taaagctttt
    40021 ccttttttgc tgaaggaacg ggttaaacgt gaggaatcaa ttccttctaa ctttttaacg
    40081 taatgccaaa caggttcacc ctcaggatag cctgttttta aaccgtcatg tttatatcct
    40141 aacgccgtta aaaggctaat aaggtcagtg tttggacgag aaatagggat gccatcagta
    40201 tcaaatgact gataattgtc gtaaggtttt aaaatgagtt cgataacgtc tttttgtttg
    40261 gcatagtctt taagttgagt gtaaaaatgt tctaattctt cgggatgatt ggtattaggg
    40321 cctgcattga gttccatacg ccaacctcca gccacttttt ggctaaagac catagcagca
    40381 acctttagtt caccatcttt ttcaagtcca agaaattgtg gttttgcccc tcttttagcc
    40441 atcaagctgg ccatttctga ggtttggata aaggaatgat ggacgagact gtggcagtaa
    40501 tggtcaaatt gttcttgtga aatttcaatt agtgccatta gttgtgctcc ttaatttttt
    40561 tcttaagtta taggttaaga tggctaagct tgaaacaagg ccgacaggaa tgttgaattc
    40621 acctgcaaat tcttcgatag tggggttgag tctagctttg aaatgataga gtccaccatc
    40681 ttgttgattt tccacacctc ctaaattatg ccagcggcag cctcttttaa aagcttcttt
    40741 agccgtttca taccaggtta aaagaggagc ttgataattg cgataatcat cgtccattcc
    40801 cgcataaaga ttttcagaag tctcgccata aatcaaggtt agggtggcgg ctaatgggat
    40861 gcgggtttgg cctgtagcga gctgctcgct taaaatggtt aaatcttttt gtagccgtgc
    40921 aatggttttt tgattttctg ctactttgct aggtttactc ttatctgtta atcgtgcttg
    40981 ttctgctaag gccttgtcca actgttggat caagagtttt ttttgttcag gtaagtctaa
    41041 actagccata gtaatgtagg actgaccagc ataaatacct agtaattttt gataatagga
    41101 tttgcctcga aggataatgc cttttcgatt ctcggttttt ttcatcaagg ctgaaaagtc
    41161 gtctagcaat tcactgccac caatcgtgac attaacacct ttattggtag cggtacgaat
    41221 ggattgcttg gcttttttgg gcaggctgtc aaagtcaaaa tccttggcgt agatgttggc
    41281 ttgtattctt ggttgaatag tgtcttcgag ttcttttgtt ctgccagacc attcgacacc
    41341 gagttttttt agaaaagcaa taatagagag tgtcacatca ttttctttgc tctcttttcc
    41401 ttctaaagtt tgtttgatga ctaaacttgg atcaattttg ataaaaagcg ctcgtttgct
    41461 ttttccgaaa gttttgagcg ttttaatgac gaaatcaagg agttcaaaat tggcgtagtc
    41521 catgattggc ccacgtggga tataaatcat agtgaaacca agaggaagtt ttctgattaa
    41581 acaagcagca gcagcaactt gtaccccatt ttcataaaag ctgatgcgtt cgtgtttcca
    41641 gttatctttg acttttcccc actttgaact ttgaagtaag ccagcttgtg gctgtgctaa
    41701 gacaaactga tcatgttctt caggagatat tccgatttta tatgtataca ttagtttaat
    41761 acccacttgc gaatagcatg tgccacacca ctgtcattgt tggatttggt gatgtatttg
    41821 gctatttttt tcaattcagg aaccccattt tccatgacaa cagggttggc aacgacttct
    41881 aacatagcac gatcattttc agcatccccg atggccatgg tttgtgacat atctagcccg
    41941 agttttttag ctaagtgttt gatggcatta cccttgctga cggtttttgg catgaactct
    42001 aaataaaaag gggttgactt gacgatggtg tacttatcaa agaaatgttg aggaatttgc
    42061 ttgatggccg cgtccaaaag gtcaggttca tcaatcatca tcattttgat gatttcttta
    42121 ttggtcattt cttcgggagt gcgatagaaa ataggcatgt ttaccaaagt cgattcatga
    42181 actgtgtatt ttccaatatt ccgattggca gtgtaaatac cttctttggt aatggcatgc
    42241 atgtgaactc ctaattttcg gctcaaaaat tccgtttcca gataatcgtc gtaagtcatc
    42301 aattctttaa caatttcttc gccggtctcg gcatcttgca ccaagccacc attaaaagtg
    42361 ataacgtggt ttcctttgtg gttgagttct aattgttcga gtaatgaaat cactccggca
    42421 ataggtctgc cagtagcaat caccacatga accccttggg ctttagcctc ttggacagct
    42481 tgaaaaacat catctgtaat tcgacggtca tcagtgagta atgtgccatc aatatcgaca
    42541 gcaaccagtt tgatagacat ggcatcctcc ttgttttttt gtggtttatc ttaaaaatgt
    42601 tctccctcct atcttactaa aaataggtgc ttttgtcatg ggatgggaaa ggaaacttaa
    42661 ggaaaaacga gagaaaacgt tttagtttag ctcaatcgcc tctgtagtaa ctaaaaagat
    42721 tgaagaaagt actctttctt caatcttaga atggagacaa acccagtttg atgctcaaaa
    42781 agcatgaact gctctgctga tttgacaact gatttcttat ttctgcgtgt gacgtgagcc
    42841 aaaacagctg ctttttgctg tggcaaaagt ggtgtcaata ctggcagtga cgttacgcgg
    42901 aatttttgag accctaggct caaaaataaa gcatagaatt ccaaaggaag tcgctgccat
    42961 aagcatcacc taagaaaagt atcaaaaaag actttttctg caatcttaaa gggctttact
    43021 gactaaagtg aaaatgccca ttggaaatat aactcataaa agtttccttg ctaggagcaa
    43081 agaggtcatc gagctctaac atttcctttg gaaaataaaa gcgacggtct ccgtaggttg
    43141 tccctgttaa tgctttgaca ataggagata actgagataa ttcagcaagg ctcccgtctt
    43201 tttgcatcat ttcaatctga gttcttggat tttcgagctc tggtcgataa atatcataag
    43261 gcaggtcgaa gttgatatgg ataccggtgt aataatctgg atcaaagcca actgattcga
    43321 ccaattggcg caagcgttct aactctcctt gggaatcttg gtcaaaggta acagacttca
    43381 gaatttttcg attaataaag cgactggcca aatctgataa gatatggtct tcgcttgcca
    43441 tccagacttg gaagtaagta ttcatgactc cgtcatctag ggctatataa tctgctaaat
    43501 tagccttctt ttcaaagaag gggatgagtc caggagctgt tttttggaaa taagcctgtt
    43561 gctcaggata taagtgttgg gctcttttga ggagattttg taagatgagc tcaacagcac
    43621 ggctggcggg atgaaaatag acctgcatgt acatttgaaa acgactgaca atatagtcct
    43681 cgacagcatg cataccacta tgttcaaaga caatgccatc ttcaacaggt cgaatcacac
    43741 gtaggatgcg cattaaatca aactgaccgt aattggcagc agaaaaataa gaatctctta
    43801 aaaggtaatc catacggtcg cagtcaatct ggctggaaat cagttgcaca acttgcttgt
    43861 taggataagt atgattaatc acgctggcaa ctttgtctgg aaaatcagga gcatgacgca
    43921 ccaaaatggc attgatttct gtctcaggat tggtaattat ctcctgagta aaggcctcgt
    43981 gatccgtatg aaaaagaacc tcaaaggtat gagaataggc tccatgacca atgtcatgta
    44041 agagggcagc cgtcatagtg accagacttt catctttatt ccagatatca gcgtatttct
    44101 cttcaaaaat agctgtcacc cgacgggcaa tttcgtaaac acctaggcag tgagaaaaac
    44161 ggctatgttc tgcgccgtgg aaggtgaaag ctgttgtagg gacttgtttg atgcgtcgta
    44221 agcgttggaa ttctttggta ttgataaggt cataaattaa tggattatca atgtggatat
    44281 agttgtgaac agggtcacga aatacttttt cattcataat ttttattata tcaaaaattg
    44341 ataaaataag gagaaattga tagaataaac agacgagctt agaatagaaa gaagagttat
    44401 gaaattacag ttaaccaacc atattcgctt tggagatgag acagagatta ttcaagaaat
    44461 tcatgattgt gagtggcgag aaaaaggggg ctatcagtac ctcatctatc aaaacactga
    44521 caaggaaaag gttgtcatca agtacaatga gacagaactg accatgagcc gtttttccaa
    44581 ccctcagtca atcatgaaat tttttgcggg caagaaggtc ctgattgctc tacctacgcc
    44641 gatgggggtt caacaatttt tgacagatac cagtcattac cacttagatt gttcttgtca
    44701 aaaattggac cttcattatc atttgcttca agctcaaaca gaaatgctat ttgcaagcta
    44761 tcatcttgag ctgagttggg attaactaaa aagatagcag taaatcatct gattagggat
    44821 tattcttgtt ttttagtcag gtttttacta taatggttat tatatcaagg gagtagcaga
    44881 cggctagccg tgattgtatc gtcaatacgg aatttcggtt tccggtacaa tcttaaacag
    44941 cgagacttgt ttaagaaaaa taaacaggtc tttttatttg aacaactaaa ataaaaagac
    45001 caaggaggta ctaatttgtc taaagaacaa agacatgaag cattttacac gcaaagtgaa
    45061 gagaccgtac tggctcaatt agagaccagt cgcgaaggtc ttacgtctgc ccaagcaaaa
    45121 gagcgcttag cagagtatgg tcgtaatgag cttgatgaag gcgaaaaacg aagtttattt
    45181 atgaaattcc ttgatcaatt taaggatttg atgattatta ttttgattgt ggcagccctt
    45241 ctttcagttc ttacagaggg gatggaaggt ttgacagacg ccattattat cttggctgtt
    45301 gttatcctaa atgctgcttt tggtgtttac caagaaggac aagctgaagc tgctattgaa
    45361 gccttgaaat caatgtcaag tccactggct cgtattcgcc gcgatgggca tgtgactgag
    45421 atagactcta aagaattggt ccctggagat attgtcttgc tagaagcggg agatgttgtc
    45481 cctgctgatc tgcgtctttt ggaagctaac tccttaaaaa tcgaagaagc agcgcttact
    45541 ggagaatctg taccggttga aaaagacttg tcaacggctg tttcagaaga tgccggtatt
    45601 ggtgatcgcg tcaatatggg atatcaaaac tcaaatgtga catatggtcg tgggattggg
    45661 gttatcacta acactggcat gtacaccgag gttgggcata ttgctggtat gcttgctaat
    45721 gctgatgaga cagatacacc attgaagcaa aaccttgaca acctttctaa gattttgact
    45781 tatgcgatcc ttgtgattgc agcggtgact tttgctgttg gagtcttctt gcgtggtcaa
    45841 cacccgcttg aaggcttgat gacttcagta gcgcttgctg ttgcagctat tccagaggga
    45901 cttcctgcga ttgttactgt tgtgctttct cttggaactc aagtacttgc taaacgtaac
    45961 gccattatcc gtaaattacc agctgttgaa acgctaggat caactgaaat cattgcttct
    46021 gataaaacgg gtaccttgac aatgaatcaa atgaccgttg aaaaagttta taccaacggg
    46081 acactccaaa gttcatcagc tgatattgct tttgacaata cgacacttcg tgtaatgaac
    46141 tttgctaatg atactaaagt tgatccttca gggaagttaa ttggtgaccc aacagaaaca
    46201 gctttggtag agtttgggtt agaccacaat tttgacgtac gtgaggctat ggtggcagaa
    46261 cctcgtgtgg cagaactgcc atttgattct gaccgtaagt tgatgtcaac cattcataaa
    46321 caagctgatg gcaaatattt catcgctgtt aaaggtgcac cagatcagtt attgaaacgt
    46381 gtcacacaga ttgaagaaaa tggacaaatt cgtcctatta cggatgctga taaaaaaacc
    46441 attcttgata ccaataagag tcttgccaaa caagctcttc gtgtcttgat gatggcttac
    46501 aaatattctg atgcccttcc aacactggaa acagaaattg ttgaggctaa tcttgtcttc
    46561 tctggtttgg tagggatgat tgaccctgaa cgtcctgaag cagcacaggc tgttaaagta
    46621 gctaaggaag ctggcattcg tccaatcatg attactggtg accaccaaga tacagcaaaa
    46681 gctattgcga aacgtcttgg tattatcgaa gaagatggcg ttgatcatgt ctttacaggt
    46741 gctgaactca acgaattatc agatgaagag ttccaaaaag tctttaaaca atactccgtg
    46801 tatgcccgtg tatctcctga gcataaagtt cgtatcgtta aggcttggca aaatgaaggc
    46861 aaagttgttg ccatgactgg cgatggggtt aacgatgcac catctcttaa aacggcagat
    46921 attggtattg gtatgggaat tacaggtaca gaggtttcta agggagcttc tgacatggtt
    46981 cttgccgatg ataactttgc aaccattatc gttgcggttg aagaaggacg taaggtcttc
    47041 tcaaacattc aaaaaacaat tcaatactta ctttctgcta acatggcaga agtctttacc
    47101 atcttccttg caaccttgtt tggttgggat gtgcttcaac cagttcactt gctttggatt
    47161 aacttggtaa ctgataccct accagctatt gctcttggtg ttgaacctgc tgagcctggt
    47221 gtgatgaaac acaaacctcg tggacgtaag tcaagcttct ttgatggtgg ggtgaaggaa
    47281 gccattcttt accaaggtgc cttccaaacc attttggtgc ttggggttta tggttttgcc
    47341 ttgatgttcc cagaacacac aagctatcat gatgtccatg cagatgcttt gaccatggct
    47401 tatgtgaccc tcggtttgat tcagttggta catgcctaca acgtgaaatc tgtttaccaa
    47461 tccatcttta ctgttggatt gttcaaaaac aaattgttca actattctat tccagtggcc
    47521 tttgtagcat taatggcaac agttgttgtt cctggattta atcaattctt tcatgtgaca
    47581 cacttgacga tcacacaatg gttagtggtt attatcggaa gtctcttgat ggttgttctt
    47641 gttgaattgg tgaaagctgt tcaacgatca cttggtcaag atgaaaaagc aatttaatag
    47701 acgctaaaag gtttgagaca attgtctcaa accttttttt ataatcgtta caatgataag
    47761 gactattggt atgcctgaca ttaaaaagtt gattcttgcc aattgacttg acagtcaaaa
    47821 atttgttgtt tggcttcttg actgtgacct tcaatatcat cgattaaaat cttagtagcc
    47881 tgttcgcctt cttcataggc gggttggata atagttgtta gtgttggtga agaaaatttt
    47941 gtccactcaa tgttatcaaa tcctactagt ccaatctctg gtatattaaa tttaagagac
    48001 ttcatagctg taaatacttt tggaagagcc caacaattgg ggacaaaaac caaactcttt
    48061 ttagtcagtg atccttgcaa aaactgagca attgcctctg aagaagtctg attttcatca
    48121 ataatcattt cttgatgagt aagatgatta gcttctaaca catcaatgaa gcctgaagcg
    48181 cgttcaatac gagtgctaag taaattgggg ttacccgtga tcatgataaa atgttcataa
    48241 cctttatcga tacattgttg aatggtatca taaacagcat cataattatt agttttgacc
    48301 cagtttgtgc gatgttcgta aagttggcta tcaaaaaaga caaccttctt ttttttaata
    48361 tcgataatac gtgaatattt tctaaagtta gaagttggtt gaataataaa accatcaaca
    48421 ccaagattga gcatcttttc aatgagttca tcttcgcgag aagggtcgta gttgctgttt
    48481 ccgatgataa tttggtaacc aaattcctgg gctttgcttt caattccttt gacaatttgg
    48541 tttgaaaaac tgtttgtgat gtcacctata acaacaccaa tgagtttagt cgattttgca
    48601 ttgagacttc tagcagcaat gctcggttta taattagtag ctttgatact ctcgctgatg
    48661 cggtttttag tttcttcaga catcttgtca aatctgccat ttaagtagaa agaaactgtt
    48721 gtttttgaag ttttcgctaa ttcagcaata tctttgatgg ttactttgcg ctgcattgca
    48781 tacactcctt taaaatagta atggttagtc atattataac ataaggactc gtaaaagatt
    48841 tagtgtttca atcgaataat ctcattcgtg atatggttga taacaaccac ctttcctctg
    48901 attttatgac tatttaggag aactagtttt tctcctttaa ttaaatcctc ttggagaaca
    48961 cagatactgt agtcaaactg ctttcctttt aaatgccagg aaagggcagt ctcaattggg
    49021 ttgcgtttgc ctgtttgaag tggtgttaag ggagtaactt tggtatcgtc agggactaat
    49081 agggtactgg tgcaaccctt gtctttaaag ggttttcgtt tgatgagttt gtgactagtc
    49141 aggtattggt tgtatttttt tgacaaaatc gtttcttcga tagtgaaagg gcagtcactg
    49201 attagtctga ggtcactaag gtgatctgtc tttacgtcta agtgattatc aagaataaat
    49261 tgactgacaa gacagtggtt gccttgagcc tgtatcgtat caatgataag aaaaattcct
    49321 ggtggtaaga tgagaatttg tcggtcatgt tggtagttgt tcctatcaga gaattgcgtc
    49381 tggtagctac cttcaataaa gtaaacgtgt ccattttgtc gaaggtgaca ataattggat
    49441 ttggggtaag aaaggtaagc ccaggagcta tcaatctgct caggtaattg ttcttctaag
    49501 aaggcggtag aatgatggct agcgcttttt agggcatagc gtaatggttc ttctttgtaa
    49561 gtgtatcgcc cagcatcaca gaaaagtggt tgtcctttat aatagagaca gagactattt
    49621 tggtcgctat gggtatggga actaccgatg ggcccatttt tgaaaaaaag ataataattt
    49681 tcttgattga tggtaatgtg cccggaatgt tcaaagtgat gcgcaaaagt tggtaaggtt
    49741 tggacgggta actgttcaaa agtgtggaca cctttttctc ctagaaggag aagactatcg
    49801 atatcaagtg tgggaaaagc agcagctttt aagtgtggtt cttcaaggat agtggctgca
    49861 agcgttagaa tatctctggt atctgtaaca tcgctatcac caagagggat ttgtttatga
    49921 tctggaccag tcattttgag gagataatca ctcatggcaa gcaaagtagg tctaagctgt
    49981 ggaagataat cagggactag agcagttaat tccagaaggg ctttgagtac ttccacatga
    50041 tacatggttg attgttcaaa ttgactgccg tcttcgagaa tttgtagtgc gatttgttgc
    50101 gtcaattctt ttctagcgaa agctgttgct gcagcaatat caaggtcaga accaaagtag
    50161 gcatcagcta ataaaatcgc agtggtttgc aagattcccc aattgctgag gctgtattta
    50221 tcaaggtagt tggcatggag aaattgaagt tgtttttcca gggaagctag gatgaggctt
    50281 tcctcttgct tggttaaggc gttaaaaaga ttaaggtaaa tcaaacattt gacccaagca
    50341 aaacaacgga tgccagtatc taaggttcta gtagctaacc ccttagggtc taaaggaata
    50401 gctgattcaa tccaattgag gatgaaacct ttagctgtta agagataccg ttcatctctt
    50461 tcaacaaggt agacgaggat taatttttgg agataggtct gtcgatttaa cataaagttc
    50521 cactcaggat catctatgac agcctcttgc caagtaatgg gatctaagtg gtaagggatg
    50581 tggcagggct ccatatccca attatcttca aataaaaagg tattggttaa gagtaggtca
    50641 gctattcttt tttgatcagc atagctgtca gtctgatagt ctagcaggta attcctgcaa
    50701 aagtctggat tgactgtttc tttaaagcgt gcgaaggcaa aaaacatagg acatttcctt
    50761 tccaaaagaa aagagacctc atcatgtgct taggaactat ttccctatgc tcataacagg
    50821 tctctatgaa ccattaagct aaaataccca accaactgcc aataacacca atgataacag
    50881 ttaaaatgac caatttgtag gttgtccatt ttttgttttt aataaggtaa tacatgagta
    50941 aggtaaagag agctggtaaa agtgcagggg caatcttatc aagcattcct tgaatagtca
    51001 caaatttttg tgctgtattg gccgcatcca ccttgccagc ggcaaaagta atgggaacag
    51061 taattttaac agaagtagct gctagtccag cgataacggt aacgccgaca atatttgcca
    51121 tgcgcgacac aaccgccatt tgttcactta atttatcaat aaagcttgtg ccgaccttgt
    51181 agccatagag tccagaacca attttaatag cggttaggat aatattcatg gctaggaaga
    51241 agaggattgg tcccaaaacc agaccatcac tagctaagga agctgcaatc gttgaaaaga
    51301 gtggcgccaa gcaaaattgt gataaggagt caccgatacc agctaaaggc cccataagtg
    51361 ccattttgat gtttcgggtt tcttcttcag ggcgattatt ttctaacatg accaagtgca
    51421 gactggtgat gaaaggaagg aagtgaggat tagtattgta aaactcacag ttatcttcta
    51481 aagcttgata taagcccttt ttatcactgc caaaatgttt tttcaaggca ggatagatga
    51541 cattggcgta tccgatacct tggtaattat tgtaattaaa cccattttgt aggaagaaag
    51601 ctctcagagc tgttttgagg taatctttct tggttaattt attagatcca gtcatcttgt
    51661 tggcctcctt ggacagttgt tgcatcgact tgtttaggtt tgttgtaaaa ttcattcaag
    51721 gcgaaaatga taccaataat ggcgatacca atggttggaa tttggaggta ggctgcacaa
    51781 acataaccaa ttagtacaaa aggaatcaat tctttcttgg ccataacaga taagatcatg
    51841 gcaaatccga tagcaggtaa cattttacca gcgacagtca atccattgag caagacaggt
    51901 gggatataat caaccaagtg aaggagagta tccattgaca aggcacctaa taaaccaagg
    51961 cctaatccga taaaagcgaa agcccagata gtgccgttgg cagcgaattt gaagcctcta
    52021 atattgcctt tttgcaattg ttttttagcg gtttcgggag cgccagcaaa agctgtatag
    52081 gcgaatgttt gaaggaattg gatggcaact gcgattggtg ttgataaggc tagtgccgct
    52141 tctggggtga ctttaccagc actggtaata gccatcaagg taccaaagat accagggccg
    52201 ataggattag gaggaacggt accaccggca cccactccaa atcccatata agctaattcg
    52261 gagatagctc ccatgctaag tgcagttggc aagtcaccta agataatccc tacaccaaaa
    52321 gacagaataa tacaacggtt ggtgtagatg ccaagtaaca ttccgctgaa acaaaaggct
    52381 gtccaaagac caattaaaag tgcctgtaac aaattgatat ccatatcaat ctcctttatt
    52441 cattaaatat aatctaagat gtcaacgtca gaggcgctat tgccagctgg agttgttttg
    52501 gtgttaaaaa ccacgtggtg gtcatgggct aagcaacgaa tagcagactt gtctgtttct
    52561 cccagcgaaa taaactgggt aatcgcaacc ttatcatcag ttttatgaat attaccgata
    52621 ttgatttctg tgatgggaac acccccttca actaggagtt tggcatcttg caaatctttg
    52681 accacgatga agatactttg agcaggactt gctttatgaa tgatgtcaat cactttttga
    52741 atggagaaaa aacgaatggc aatactggat ggaataactg ttttcatgag ggattgttga
    52801 atcttatctt cagaaaccgc atcgttagcg acgataacgg tgttgcagtt gaggaatttg
    52861 acccataatt gcccctgtcc gtgaatcaaa cgctcatcaa ctcgcgtcat gataatgttt
    52921 ggttgtgtca taaaatctcc tttttaaatg taggtatcgg tgtttgttac cagtaagggt
    52981 tccagtcttt gtagaaacga agcagagctt caagatagta atagtctccc cagatatttc
    53041 cctcgtcaac cccttttcca gagtgccaag aatagactcc gtgaagcagt agaggagcac
    53101 caggcttgag atctttgtta gcataatcct taatcaaggc tcttagcatg ctgtgcatgg
    53161 cagcttcata tgttttttta tcaggatcat gatctggtaa ggtttttaac atttcatgaa
    53221 taccacacac agcaatggcg gttgctgaac tatcacgaga ctgctcgcta ccatctccaa
    53281 aaattaaatc ccagtaggac acctggtctt taggaagacg gttcaagaaa taatgggtca
    53341 tgcccttgaa gagttgaatg agttcaggtt cttttaggaa acgataggtc aaaggaatac
    53401 cataaatgcc ccaagcttgc cctctggccc aagcagaatc atcgctataa ccttgtcggg
    53461 tgaccccttt aacaggatca cctgtttctg gatcaaagta gaaggtgtgg taagcagagg
    53521 cgtcatctcg gatgacatga ttggctgagg cgtagaaatg attgatcgcc atgtcacggt
    53581 aacggttgtc tccagtttct tggctagcaa aaaagagtaa ttggatgttt aggagacaat
    53641 caatgattaa acggtaatct tcttttttac ccagttctcc ccaggcctga ataaagccgc
    53701 ctttgtcttg atagcgttgg actaatttgt cggctgcttt caaagcagct tctctagact
    53761 caggtgtttt taaaagtttc cactcagcca tgcaagaagg ggtgtaaaga aagcccaaat
    53821 catggtgatc tagctcaatg tctcttgtta ctcgatctag gaaagaaagg tcatttgctt
    53881 gagccagagc tttaatagca tcatccccac tgtattcgta agctaaccaa aggcaacctg
    53941 tccaaaaggc attggtccat tctgtattat ccataatggc atattggtta tctttggtag
    54001 ctggcgttgg aaagtcttct ttaaaatagt ccatattaag ccgaacttgt ttgagggcta
    54061 agtcaagggc ctgagtaatg tcttcttggc ttaggaaatc ttctttggta aatcgttcag
    54121 gctgcttgat aggttcaaga gcaatggttt ttaaaggtcg tgccattaaa tgcctcctaa
    54181 ctcatcgtct gtcgcaccgt cttcttgctg agaaagagtt ttccagtcaa caatgccttc
    54241 tttagaacat gttataagtc ctgatactaa gtcgtcaaaa gaagctgctg tttgtcttgc
    54301 aaaactagct tcaatcagca tggctaagtt aaggccagaa agaacattac acgtcgtgtt
    54361 tggaagagat tccattaagg ttgcagcgac tttaaaaggg gtacctccta gaaggtcaca
    54421 aagaactaag gtttcctctt ctgggattaa agcacgagat aattggtcct gcacatcagc
    54481 ggcggtcatc tcagtagtaa aatcaattgc agttaccttt tcttgtttgc ctgcgatcaa
    54541 ttctaaggct gaaacaatac cactcgcaaa atgtccatgt cccataacga taatagcaat
    54601 cataaataaa tctccttcgg tttaccggtt tactcaaaaa tattgtagcg ctttcaaaat
    54661 tctttgtcaa tactttttta aaaagatttt tgaaaaagaa aaagtgaacg gttgtgctaa
    54721 aaaagaatca aaaatggtct ttttttgtca ctatttaatg tagaaaagtc ttaatggcta
    54781 ttaagtatag gagaagacta aacaagaatg gcagtttgga taagagcact cttttaaatt
    54841 ttgaaccgtt ttcattaaaa tgattgacaa aactagttta ctttgttata gtcttggtaa
    54901 acaggtttac ttaatttaat aacaaaaagg agaatcctat ggaaaatatg ttttcgttac
    54961 aaggtaagat tgccttgatt accggagcat cttacggtat tggttttgaa attgccaaag
    55021 cctatgctca agcgggtgca actattgtgt ttaatgacat caaacaagag ttagttgata
    55081 aggggttggc tgcctatcga gagttaggga tagaagcgca tggctatgtt tgtgacgtga
    55141 ctgatgaagc tggtatccaa cagatggtta gtcaaattga agatgaagta ggtgctattg
    55201 atattttggt caacaatgca ggtattatta ggcgtacgcc gatgcttgaa atggccgcag
    55261 aagattttcg tcaagttatt gatattgatt taaatgctcc ttttattgtg tcaaaagctg
    55321 tattaccatc aatgattgca aagggacatg gtaaaattat aaatatctgt tctatgatga
    55381 gcgaattggg gcgtgaaaca gttagtgcct acgcggcggc taaaggtggc ttgaaaatgt
    55441 taaccaaaaa tatagcctct gagtttggag aagccaacat tcaatgtaat gggattggtc
    55501 ctggctacat tgcgacgccc caaacagctc cgttgagaga gcgacaagca gatggtagtc
    55561 gtcatccttt tgatcaattt atcattgcta aaacaccagc agcacgttgg gggacaacag
    55621 aagacttagc tggtccagct gtttttctag ctagcgatgc cagtaatttt gtcaacggcc
    55681 atattcttta tgtcgacggt ggcattttag cttatattgg aaaacaacct taatcacaca
    55741 ctagaaagag attttatatg aaaattgcat taattaatga aaatagccaa gcggctaaaa
    55801 atggtatcat ttacgatgcc cttaccacag tgacagataa acacggctac caagtcttta
    55861 actatggtat gtatggcaca gaaggtgaaa gccagttgac ctatgttcaa aatggactct
    55921 tagcttctat tttattgacc actaaggcgg ctgattttgt ggtgactggt tgtgggacag
    55981 gtgttggagc tatgttggcc ttgaacagct tcccaggtgt tacctgtggt tttgcgagtg
    56041 agccaacaga agcttatctc ttttctcaaa tcaatggtgg taatgccttg tccattcctt
    56101 tttccaaagg ttttggttgg ggagcagagt tgaatttgac cttaatattt gaacgcctct
    56161 ttgcagaacc aatgggtggt ggttacccta aagagcgagc tattcctgaa cagcggaatg
    56221 cacgtatttt aagtgatctg aaaaaaatta cttacagaga tttgttggca attgtcaaag
    56281 acatcgacca agatttcctc aaagaaacga tttctggcgc tcatttccaa gagtacttct
    56341 ttgccaatgc ggagccgtca gaattagtca cttatttgaa atcagtactt gaacagtaat
    56401 tccattttga cgaggaaagg aggtcacatg tctaagttat tattggttgg tgaaccgctg
    56461 attcgcgtta gccccaatca gtttcagcct ttgaccaatg cctgcgaggc tcagcttttt
    56521 ttcggcggtt cagaagttaa tattgcaaga actctaggcg gttttggctt agaagcaagg
    56581 ctatttacgg ccctaccaga taatcctgtt ggtcacgctt ttcatcagtt tttgaagcaa
    56641 agcggcgtgg atatgtcctt gacggcttgg caaggtcatc gcgttgggct atattacctt
    56701 gaaaatggct ttggctgtcg ggctagtcag gtttattatg accgctgtgg ctctagcttt
    56761 tcggccttag ataaggatag tcttgattta gctgccattt ttgaaggcat tagtcacttt
    56821 cattttagtg ggatcagttt ggcgttgggc aaaaaaactc aggacctgat tgaggtactt
    56881 gtgagagaag ctaagaaaag ggatatttgt atttcctttg accttaactt tagatctagt
    56941 atgattgccg tagctgatgc taaacgactc ttttctcact ttgctcaata cgcggatatt
    57001 atttttggca tggaaccttt gctgttagac agtgacgatt ttgacatgtt tgatagaaaa
    57061 aaggcagata ctacgacgat tagagaacgt ctggcaggac tttatcagcg ctatcagtta
    57121 caagctattt accatacgga gcgttcaaat gatgcccaag gcagtaatca tttcaaagct
    57181 tatgcctatg ataggcagtt ttacgaatcc tgtgaagtga cgaccccagt cttgcaacgg
    57241 gttggaagtg gagatgcttt tgtggcgggt ttactttatc agttgttaga aggtaacgaa
    57301 aagcagcgca accttgattt tgctgtggca acagctagcc taaaatgtac agtagctgaa
    57361 gatcagctct atcacagtgt ccaacaagta gaagcagtct tagctaatca acgagatatc
    57421 cagaggtgat gatatgggaa aaatagaaat cttaaccaaa cttaaggcta atcggcttgt
    57481 cctagttgta cgaggagagt ctagtgagga agctttagct tgcagcctgg ccagtattga
    57541 aggtggcatt aagacaattg aagtcaccta tactaacccc tttgctagtg aggttatcgg
    57601 tcaattagct gaacgcttca aagaagatcc tgaggtattg ataggtgctg ggacagtttt
    57661 ggatgatgtc actgcccgcc aagctatttt agcaggagca cagtttattg ttggtcctaa
    57721 tttcaatcga gcagtggctt tgatttgtca tcgttatagc ataccttatt taccaggttg
    57781 tatgacggtt aatgaagtgg ttacagcatt agaatcaggg gttgatatgg tcaaaatttt
    57841 tcctggttct actgtaggga tatcctttat tcgggcaatc aaatctcctt tgccacaagt
    57901 agaggttatg gttacaggcg gagtttcttc agataatctc aaagactggc tagctgctgg
    57961 agtagatgtt ctaggcatag gaggcgagtt taatcagtta gcgagccaaa aacaatataa
    58021 cctaatcact aagaaagccg ctcattatat caaaagtttg cgacaataat ctaaaaaacg
    58081 tttttacctt ggctttagtg ctagtaaaaa cgttttttta tcattcattc aattaatatg
    58141 cttctgctca tgtttttcca gatagtatag atataagagc agctaattta acaagatgag
    58201 cctgtcgttt gatatatagg atataaactt tttatgatca aacaaaagta ataccttttg
    58261 aattcgttag tattgatctg atctaaagtg atatcactta gaatggacta gctgtacagc
    58321 ttttacagca acttatgcta aaatagaaaa aaggagaaat gttgatgatt aaaggaatta
    58381 tttttgatat ggacggtgtt ttatttgata cagaaccttt ttatctgagg cgacgagaag
    58441 atttttttaa gacaaaggga attcccattg atcatttgaa ctctaaagat tttattgggg
    58501 gcaatcttca agaattatgg aaagagttgt taggtaaaaa tagggatgat gctatcgtta
    58561 aggcaattac aactgactat gacgcttaca aacaagcgca taagcctcct tatcaaaaac
    58621 tgttgattac agaagtgaac tcttgtcttg aacagttgga aaaacaaggt attaaactgg
    58681 ctgtggcatc aaactcgaag cgtcaggatg ttttgttggc gttggagaca acgcaaataa
    58741 aagattattt tgaaataatc ctagcgcgtg aagatgtttc tagaggcaaa ccttatccag
    58801 atatttataa taaagcagta caaaaactag gattacaaaa aaaacaactg cttgtagtag
    58861 aggacagcca aaaaggcatt gctgccgcca aagcagccaa tctgacagtt tttgccatta
    58921 ccgactaccg atatggcatt gatcagagtc aagctgatca caagatagat catttaggac
    58981 aactgtgtgt aaaaatcggt tgttttggat cgtgaagtaa cgcaactaac tgatcatgat
    59041 acttcaagac agttaagaat acgtctagga ctttctacag accaataaca agaatatgtt
    59101 tatgtgtttt tactgttttt tagctaccta tttacaatat gacactgaaa agagtactat
    59161 gtttaagtta ttatcaaaaa aaacgtcgca taagatcacc tagaaaagga ttatgatttt
    59221 tgtgatttgg atgcgatcat tagtcgtcga ccatcttcat ttggcaggag gtatgtttaa
    59281 ccgtggtcat tatttggatt tgtttttaga ttggctagtg agtaaatata gaaggatcat
    59341 aatactgctt tgacaaaggc agtatctgag tccatgagtt gcacgggtaa tgtgactcgg
    59401 ttttggttag gtataataat atttggaagt gttatgctgc ttgtcttcgt aggtccattg
    59461 attatagtca gacttacttt agagctgagc tgactgtcta gacaagacgt gatggagatg
    59521 tgatttctga ccaagtagag gtaaccagaa aagggacttt tacgatggaa ttgaatttac
    59581 ttgttgcatt cttgtgcatg ttaagagaga aaacccagat aaatcaggaa gtaaaatggt
    59641 tatgtttgat tagaattgtc cttctttttc cttgaagtct taacttattt tgattatgat
    59701 agtagtatga ctattaaggc agaaattaag gcgctcgcca aagaaattgg tatctctaaa
    59761 attggtttta cgacggcaga taactttgat tatttggaaa agtcattgcg tgcttctgtg
    59821 gaagaggggc gcaattcagg atttgaacat aaagtcatag aggatcgtat ctacactgaa
    59881 aggcttttag aatcggctaa aacgattatt tcaattggcg ttgcttatcc tcacaaactt
    59941 cctcagcaac ctcaaaaaac accttataaa cgtggaaaaa ttacacctag ttcgtggggg
    60001 cttgattatc attatgtggt cggcgaaaag ttagacagac tatcaaaagg cattgaagag
    60061 ctgtgtcgag attttccttt gcaacaaaaa gcaatggtcg atactggagc tttagttgat
    60121 actgcagttg cccaaagagc gggtattggt tttataggaa aaaatggtct agttatttct
    60181 aaagaatatg gctcttatat gtttttggga gaattaatta ccaatttaga gatcgagcca
    60241 gacaagcctg tcgattatga ttgtggtgac tgtcgtcgtt gtctagatgc ctgtccaact
    60301 tcttgtttaa ttggtgatgg ctcaatgaat gccaagcgtt gtctatcatt tcaaacccaa
    60361 gataagggaa tgatggacat agagtttcga aaaaagatta aaacagttat ctatggttgt
    60421 gatatttgcc aaatttgctg tccttataac aagggtataa acaattcacc agctacagag
    60481 attgatcctg agttggctca accagaatta ataccatttt tgagtttatc aaatggaaaa
    60541 tttaaagaaa aatttggtat gattgctgga agttggcgag gtaaaaatat tttacagcgt
    60601 aatgccatta ttgcgctagc aaatgctcat gataaaactg cagtggtcaa attaatagag
    60661 attattgata aaaataataa ccctattcat actgcaacag ctatatgggc attgggagaa
    60721 attgtcaaaa aaccaaacga tgagatactg gcgtttatga gtcatttaac cttaaaagat
    60781 gaagatagcc gaaaagagtt ggaattaatc cgtcataaat ggcaatttta agccttttat
    60841 ggtattttta agaaccttat gataaaataa aacagattaa caaagattgg agaaatctca
    60901 tggaagtggc tgaaattcgc caaaaaatag tagaaaataa agagaagttg actagcttca
    60961 ggaggtctct ttgacttaga tcgtttggaa gaggagattg ctcttcttga aaatcacatg
    61021 actgagccag acttttggaa tgacaatatt gcagcgcaaa agacatctca agaactaaat
    61081 gagttaaaag gtaagtatga tactttccat aatatgcaag aattatctga cgaaacagaa
    61141 ctcttacttg agatgcttga cgaagatgat tctttgaaag aagaattaga agaaaatcta
    61201 atgcagctag ataagataat gggtgcttat gaaatgacgc ttctcttatc tgaaccctat
    61261 gaccacaata atgctatctt agaaatccat cctggttcag gaggaactga agcgcaagat
    61321 tggggagatt tactcttacg aatgtatacc cgttttggca atgctaatgg tttcaaaata
    61381 gaagtattgg attatcaagc aggagatgaa gctggtatta aatcggttac cctctctttt
    61441 gaaggcccca atgcttatgg tttgttgaaa tcagaaatgg gagttcatcg cctcgttcgt
    61501 atatcacctt ttgattcggc taaacgtcgt catacctcat ttgcttcagt tgaagtgatg
    61561 ccagagttag atgatacgat tgaagtagaa gtacgtgatg atgacattaa aatggatact
    61621 ttccgttccg gaggagctgg tggtcagaat gtcaataaag tatcaacagg tgttcgtttg
    61681 acgcatattc caactggaat tgtggtatcg tctaccgttg accgtactca atatggaaac
    61741 cgcgatcgcg ctatgaagat gttgcaggct aagctatatc agttagaaca agaaaagaaa
    61801 gcacaagaag tagatgctct caagggtgat aaaaaagaaa tcacttgggg aagtcagatt
    61861 cgttcttatg ttttcacccc ttatacgatg gtgaaggatc atcgtactaa ctttgagctt
    61921 gctcaggtag ataaggttat ggatggagag atcaatggct ttattgatgc ctaccttaag
    61981 tggcgcattg aagactaaaa ataataaagg agaaacatgg cactaataga aatgtctggt
    62041 gttactaaaa aataccgccg ctcaacgacc gccctcagag acgtcaatgt ttctgttaac
    62101 caaggtgagt ttgtttatct cgtaggccca tcaggtgctg gtaaatcaac ttttatcaaa
    62161 ctcctttacc gtgaagaaca actgacaact ggtaagctat atgtgggtga attcaattta
    62221 actaagctaa aagcacgtga tgttccgatt cttcgtcgtc acatcggagt agtttttcaa
    62281 gattacaaac tgctaccaag aaaaacagtc tttgaaaatg tagcttacgc catggaagtt
    62341 attggtgaaa agcgccgcca cattaaaaaa cgcgtgccag aggtactcga tttagttggc
    62401 ttgaaacata agatgcgttc ttttccaagt cagctatcag gaggggagca gcaacgtgtg
    62461 gccattgcgc gtgcgattgt taacaaccct aaattattga ttgcagatga accgacaggg
    62521 aatttagatc ctgagatttc atgggaaatt atgcagttac ttgaacgtat taatgttcag
    62581 ggaacaacta tcttaatggc gacacacaac agtcatattg tgaatacatt ccgtcatcgt
    62641 gttgttgcta ttgaagatgg tcgcatagta cgtgatgaag aaaaaggaga ttatggttac
    62701 gatgattaga tactttttcc gtcatatttg ggaatccatt aaaaatctta aacgcaactt
    62761 ttggatgaca tttgcttcag ttagtatggt ggctgttaca ttgactttag ttggtgtttt
    62821 tgcggcgaca ttgttgaata tacagcgtgt tgcttcgggt gttgaaaata atgtccatat
    62881 caatacctac ttacaagttg attcaactga tgctgcaaaa gttattcaaa atactgccgg
    62941 tgaacctgtt aataacgata actaccacag tgtttacgac aaaattgctc agataaaagg
    63001 tgtcaaaaag attacatttt ctagcaagga tgaacaattg aaaaaattgc aagaaacctt
    63061 aggtgatgtt tggaatatgt atgaccaaga cactaatccg ctccaagata tctatcttat
    63121 tgagacccag acgcctaaac aagttaaggc gatcactaaa aagatcagaa cgattgaagg
    63181 cgtagaagcc gcagattatg gagggattaa ctccgataaa ctatttaagt tttcaacatt
    63241 aattcaaact tgggggctga tcggaacagc aatgctatta tttgtagctg tctttctcat
    63301 ttcaaacact attcgtatga ctattatgtc tcgtaaacgt gacattgaaa ttatgcggtt
    63361 agttggtgct aaaaattctt atattcgagg gcctttcttt tttgaagggg cttgggttgg
    63421 cttgttagga gctgttttac catctcttct gatttattat ggatatgatc ttgtttataa
    63481 gcattttgca caagaattac aacgtaataa tctctcgatg tatccgcttg atccttatgt
    63541 atattacctg attggtgctt tgtttgtcat aggtattatg attggttcgc ttggttcagt
    63601 gctttctatg agacgttatt taaaattcta aaaaaagcgc tgctgacgaa acgtagcgac
    63661 cctcaaaagt tggacctctt atgttattcc aaggacctcg ttttgtattg cacaggactg
    63721 gtccttttca ttttgttttg attcatttgt ttatttgtaa taggcaatac agtttaaaat
    63781 agcagtttct aaggtttcaa gagaagaaaa gcaccccaaa agttagatgt ttgatgtatc
    63841 taacttttgg ggtgtcattc aaaatttagt ggctcttttt tagtaaaata ctatgatttc
    63901 agttttagga taacctcaaa cagtatctaa aatagctatg tcaatgaaaa aaggggttag
    63961 catttttttc gtgacaaatg gtagtagaag gaccatgccc aggataaact cggtaatgat
    64021 taggtaaggt gaatagttct tggcgaatac cagtaatgag gtcctcaaag ttactggtag
    64081 gtaagtcagt acgaccgata gtttcacgaa atagagcatc gccagtaacg actaattcat
    64141 ctgagtggaa aacaaatgag actcctcccc aagaatgacc agatgttggt aaaactgtga
    64201 attcaaaacc atttagttga tagggttgct ttaatttaaa aaagttctct gccggtcttg
    64261 ctataacagt aatgatatca tcatgacggc ctaagccaga taaattatca tctggggaag
    64321 atagccaagc ggcttctttt tctgaaacat aaacaggtgg atgatcaaaa gcatctctga
    64381 ccaaatctaa actaaaaata tgatcatagt gagtatgcgt taagaggata gccactaaag
    64441 gtttttcaaa ggatttaatt ttagcaatga tttcttggcc gttacttcct ggatcaatga
    64501 ggataacagc ttgatcatta acgaggtaat aagtattttc accagcaatg tggtttattg
    64561 ttttatagat tttcataagg atagtgtacc aaaaaatcaa gactttgtta aaaaaagagt
    64621 agcggaaaat aaatggcaat tagtttcaga aaatatgaga tctcacagca atttcttgac
    64681 accgttttca taaaggtgta tcataaaagt aatcaataag ccaagcctaa agctgaactt
    64741 agtttaagaa cactgacttt ttgagagcaa cctaatcggt tgactaaaaa atatgaggtg
    64801 gaagatatgt caaaagttgc tattgttact ggtgctggtc aaggaattgg atttgctatt
    64861 gcgaaacgtc ttcatgcaga cggctttaaa ataggcattt tagactataa tgaagagact
    64921 gctcaggctg cggttgataa actatcacct gaggatgctg ttgcagttgt agcagatgtt
    64981 tcaaaacgtg accaagtttt tgatgctttc caaaaagttg tagatacttt tggtgatttg
    65041 aatgttgttg ttaacaatgc aggcgttgcc ccaacaaccc ctcttgacac gattactgaa
    65101 gagcagtttg aaaaggcttt tgcaatcaat gtcggaggaa cgatttgggg aagccaagca
    65161 gctcaaaaac attttagaga attaggtcat ggcggaaaaa ttataaatgc aacatcacaa
    65221 gcaggatgtg aaggaaatcc taatttgact gtttatggtg gtacaaaatt tgcagttcgt
    65281 ggtattacac agactttggc aaaggatttg gcatcagaag gaatcacagt caatgcttac
    65341 gcaccaggta ttgttaagac acctatgatg tttgctattg cccatgaagt tggtaaaaat
    65401 gcaggcaaag atgatgaatg aggtatggag caatttgcaa aagatatcac tttgaaacgc
    65461 ttgtctgaac cagaagatat tgctaatgca gtaggcttct tagcgggaga tgattcaaat
    65521 tatatcactg gacaaacaat agttgtcgat ggtggtatgg tattccatta aaaatgatta
    65581 atactgacac tgtgggtttc actcacagtg ttttttaaga agtataaaaa attatcctat
    65641 cttggttatt ggagtgatgg cacctttgag gtatagtcat gttttgtttt atagatatag
    65701 cgtgctataa tagacttact atgacccaga aaaaacttag aaaatatgca gtagttgact
    65761 tggaggctac tggagcaggt cccaatgctt ctattattca ggttgggatt gttattatcc
    65821 aaggaaataa aattattgat agttatgaaa cagatgttaa tcctcatgaa tctttggatg
    65881 agcatattgt tcatttgaca ggtattacgg ataagcagtt ggctaaagct ccggattttg
    65941 gtcaagtggc gcatcacatt tatcaattga ttgaagattg tatttttgtt gcccacaatg
    66001 taaagttcga tgcaaattta ttggcagaag ctcttttttt agaaggatat gagttaacaa
    66061 taccacgtgt tgatactgtt gaattagccc aactattttt ccctcgcttt gaaaagtata
    66121 accttagtca cctatctagg cagctcaata ttgatttggc agaagctcat actgctattg
    66181 cagatgcaag agctacagct atactgtttt taagactttt acaaaaaatt gagagccttc
    66241 caattgagtg cttagaaagc ttattagtct attcagatag cctgctattt gaaacagcaa
    66301 tggtcattca agaaggatta gccaaagcca aaccttatga tcccaacaaa tacattaaaa
    66361 tcagacagat tctcttacca aagggctcaa aagctctcaa gccttatcaa atatcaaaaa
    66421 gcttccctat taatatggca ctattaggtc tggaagaaag gcctaaacag actcaatttg
    66481 ctcagttaat tgacgaagat taccatcagg gagtagctag ttttattgaa gctcagacag
    66541 gtattggaaa aacctacggc tacctgctac cccttttggc aaaagaagat caaaaccaaa
    66601 tcattgtcag tgtgcccacc aaacttcttc aagaccaatt aatggctggt gaagtagctg
    66661 ctattcaaga acaatttcac atcgcctgcc atagtttaaa agggcccgcc aattacctaa
    66721 agctagatag ctttgcagat agtttggacc aaaatgacca gaaccgtttg gtgaatcgtt
    66781 ataaaatgca attattggtt tggctcttag aaactaagac aggagacctt gacgaaatca
    66841 aacaaaagca gcgctttgca gcttattttg agcagcttaa gcacgatgga gacattaagc
    66901 agtcttctga attttacgat tacgattttt ggagagttag ttatgaaaaa gccaaaacag
    66961 cccgtctcct gattaccaac catgcttatt ttttacaccg tgttcaagat gacaaggact
    67021 ttgctagaaa caaggtcttg gtctttgatg aggcccaaaa gctgatgctc caattagatc
    67081 aattatcacg tcatcagctt aacctgacgg tttttttaca aacaatacag gccaagttaa
    67141 gcaacccttt acctctttta gaaaaacgct tattggaaag cttgtcgttt gaactgggac
    67201 aagtgtcaag tgattactat caaaataaag agcaccagct tgctcatgat tggtctcgta
    67261 tagctggcta cgccaaagaa ttaacaggag cagattatca agagttacaa gccttttttg
    67321 ctacctcaga tggtgactac tggcttagta gcgaaaagca agaggagaag cgagtgactt
    67381 atctaaacag tgctagtaag gcatttattc atttccaaca gctcttacca gaaacggtga
    67441 aaacttattt tgtttctgcc actttaacca tcagctcaga agtaaccttg gcagacctat
    67501 taggttttga agagtatctc tatcatgtca ttgaaaagga taaaaagcaa gaccagttgg
    67561 tgcttgttga tcaagaggcg cctatcgtga cagaagtcag tgaccaaatc tatgtggaag
    67621 caattgcaaa acgaatcgaa agcttgaagc aagagggtta tcctatttta gtgcttttta
    67681 attctaaaaa acacttgctg ttggtttcag actatctaga ccaatggcag gtgcctcact
    67741 tagcgcagga aaagaatgga acagcttata atattaaaaa acggtttgat caaggagagc
    67801 aaactatttt gcttggttta ggttcttttt gggaaggagt tgattttatt caagcggatc
    67861 gtatgattac ccttatcgca cgattgccct ttgataaccc agaggatttc tttgttaaga
    67921 aaatgagtca ttatctattg gaaaaaggta aaaatccttt cagagattac ttcttgccaa
    67981 tgactatttt gcgattaaaa caggctattg gtcgcacgat gcgtcgacaa gatcaaaagt
    68041 cagtggttat tattttggat cgtcgtctcc ttacaaagtc ctatggacaa gttattttgg
    68101 aaggtctcgg tcaggaattt ttgatttctc aacaaaattt tcacgattgt ctagtagaaa
    68161 cggactgttt tctgatataa tggaaaggat ctattatatc agaaaaattt ttaatggcgt
    68221 taagcacaat caactaacat ttaaattagt ttgaactttt attatgctct tttagacaac
    68281 tgttcaaatg ttttcgaatt gtttttttga taaggacaat aaatgaacaa atttttggtt
    68341 ttagtaaacc caattactgt tcttttcatt agtgtcagtt gctatcaaaa cagcttgtca
    68401 tagttcgtgc gatcagtagc gagtattggt aaaactgaaa ccgtagatta tgaaaaaatg
    68461 atctgttgag cattaattaa tctgaagata agaagtcgat aaaaaggaga tacgatgccc
    68521 aaactatcaa agcgagtttt agaaatgaaa gagagtgtta ctctggctgc aggtgctaga
    68581 gctaaggctt tgaaagcgca gggccgcgat gtactgaatt taactttagg agaaccagat
    68641 ttttttacac ctaagcacat tcaagacaag gcgattgaat ctattcaaaa tggtacggct
    68701 agcttttata caaatgcttc tgggcttcca gaattaaaag ctgcgattgc cacatacctg
    68761 aaaaatcagt atgggtatca cttgtctcct gatcagattg tggcaggaac aggtgccaag
    68821 ttcatccttt atgccttttt tatggctgtt ttaaatcctg gtgaccaagt tctcattcca
    68881 acaccatatt gggtatctta ttctgaccaa gtaaaaatgg cagaaggtca gccgatcttc
    68941 gttcaaggac tagaagaaaa tcaattcaag gtaactgttg accagctgga aagagcaagg
    69001 acaagtaaga ctaaagttgt ccttatcaat tctccttcca atccaacagg catgatttat
    69061 ggtgctgagg aattaagggc cattggagaa tgggcggtgc acaacgatat tcttattctg
    69121 gcagatgata tttacgggtc cttagtctat aacggcaatc agtttgttcc tatttcaaca
    69181 ctctcagaag cgattcggcg tcaaaccatt acggtcaatg gcgtggctaa aagttatgcc
    69241 atgacaggct ggcgggttgg ttttgcagca ggtgaaccag agatcatttc ggcaatgagt
    69301 aagatcatcg ggcagactac ctctaacctg acaaccgttt cacaatatgc tgctatcgag
    69361 gctttctgtg gtagtcagtc atcacttgaa gagatgcgat tagcatttga agagcgcctt
    69421 aatatcactt atccattgtt gtgtcaggtc cctgggtttg aggttgttaa accacaagga
    69481 gctttttact tctttccaaa cgttaagaaa gccatggaaa tgacaggttt ttccgatgtg
    69541 acaagctttg ctaatgctat tttagaggaa gtcggattag ctgttgtgag tggagcaggc
    69601 tttggcgcac cggaaaatgt tcgtctgagt tatgctacag atatcgaaac ccttaaagaa
    69661 gctgttcgta gactgcatgt ctttatggaa agtaatgaaa ttaattaaat aaaaatagag
    69721 agaaacttat gtcaaaaaaa cttatttcaa ttgttgatgt taaagattat gttggtcaag
    69781 aagttactat tggtgcttgg gttgctaaca aatcaggtaa aggtaagatt gcgtttgtac
    69841 agttacgcga tggttctgct tttttccaag gtgtcgcttt taaaccaaac ttcattgaaa
    69901 aatatggtga agaatcaggt cttgaaaaat ttgacgttat taaacgtctc aaccaagaaa
    69961 catctgttta tgtaacaggg attgttaaag aggatgagcg ttctaaattt ggctatgaac
    70021 ttgatattac tgaccttgaa atcattggag agtcacatga atatcctata actcctaaag
    70081 agcatgggac agacttcttg atggacaacc gtcatctttg gttgcgttct cgtaaacaaa
    70141 tggctgttat gcaaattcgt aacgctatta tttatgcaac ttatgaattc tttgaccaaa
    70201 atggttttat caaatttgat agcccaattt tatcagaaaa tgcagcagaa gattcaacag
    70261 agttattcga aacagactat tttgggaaac cagcatttct tagccaatct ggtcaattat
    70321 accttgaagc tggagcaatg gctctaggcc gagttttcga ctttggtcca gtctttcgtg
    70381 ctgaaaaatc taaaacacgt cgccatttaa cagaattttg gatgatggat gctgagtatt
    70441 ctttcttatc acatgaggaa tcacttgacc ttcaagaagc ttatgttaaa gcattgatcc
    70501 aaggcgttct agatcgtgcg cctcaagcac ttgatatttt agaacgtgat gttgaggctc
    70561 tcaaacgtta tatcacagag ccattcaagc gtgtttctta tgatgatgct attacacttc
    70621 ttcaagagca tgaagcagat gaagatacag attatgaaca tttagagcat ggtgatgact
    70681 ttggttcccc acatgaaact tggatttcta attactttgg agtaccgaca tttgtggtga
    70741 attacccagc aagctttaaa gcattttata tgaaaccagt tcctggaaat ccagaacgcg
    70801 ttctttgtgc tgatctttta gctccagaag gttatggaga gatcattggc ggctcaatgc
    70861 gtgaagacaa ctacgatgct cttgtggcta aaatggatga actaggtatg gataaatcag
    70921 aatatgactt ttaccttgac cttcgtaaat atggctctgt tcctcatggc ggttttggta
    70981 taggtattga acgtatggta acttttgttg ctggtaccaa acatattcgc gaagctattc
    71041 cattcccacg tatgttacat cggattagac cctaataata caaaaagttc atgtcatcta
    71101 tgaacttttt gtattggtaa gaagtgagtg tgttataaaa acatcaatat gtcctaaagg
    71161 agagataaga aatagtagag tttgaaccta catcctttgt gaagataatt tttggaagtt
    71221 tagtagatca gttagcagat aagacaatat agctttgctc aagggatatt ttatagacaa
    71281 gtatttggac tacgattatc aaatagacac tctcatttga tactaaaaag gtctcgtcta
    71341 ttcatataat aggagagcta cgggagttat tattaaaatt agctgatttt tacagttcaa
    71401 aatgtgttac cactttttca cttcttagct ttcttatgta tgtggtataa tgaaggtgga
    71461 cggttcgata gagaaaggga aattgtaaat gtcagacaaa cacattaatt tagttattgt
    71521 gacaggaatg agcggcgctg gaaaaacagt tgccattcag tcttttgagg atctaggcta
    71581 ctttaccatt gataatatgc ccccagcctt ggttccaaaa tttttagaat taattgaaca
    71641 aaccaatgaa aatcgtaggg tggctttggt tgtcgatatg agaagtcgtt tgtttttcaa
    71701 ggaaattaat tctaccttag atagtattga aagcaatcct agcattgatt ttcggattct
    71761 ttttttggat gcaacggatg gagaattggt gtcacgctat aaagaaacca gacggagcca
    71821 ccctttggct gcggacggtc gtgtgcttga tggtattcga ttggaaagag aactcctatc
    71881 tcctttgaaa agcatgagcc aacatgtggt ggatacaaca aaattgaccc ctagacaatt
    71941 gcgtaaaacc atttcagacc agttttctga agggtctaat caagcctctt tccgtattga
    72001 agtgatgagc tttgggttca aatatggtct tcctttggat gcggatttgg tttttgatgt
    72061 gcgttttcta cccaatcctt attatcaggt agagcttcgt gaaaaaacag gactagatga
    72121 ggacgttttt aattatgtga tgtctcaccc agaatcagag gtgttttaca agcatttgtt
    72181 aaaccttatt gtccctatct taccggctta ccaaaaagaa gggaagtctg tcttgacggt
    72241 ggctattggc tgcacaggag gccaacaccg cagcgttgcc tttgcccatt gcttggcaga
    72301 aagtctggca acagattggt cggttaatga aagccatcgt gatcaaaatc gtcgtaagga
    72361 aacggtgaat cgttcatgaa aaatcctaaa atgactgtta taggtggggg tactggcatc
    72421 tccattatcc taaaaagcct tcgtaatgaa gcagttgata ttactgctgt ggtcactgtt
    72481 gcagatgatg gtggttcctc aggagaactt cgtaatgcca tgcaattagc tccgccagga
    72541 gacttacgga atgtcttgtt agcgatgagt gacatgccaa aattttatga gcgggttttc
    72601 caataccgtt ttaacgagag tgatggagcc ttggctggtc accctctagg caatctcatt
    72661 attgctggta tctctgaaat gcaaggctca acctacaatg ccattcaaat tttaaccaaa
    72721 tttttccata ttacaggtaa aatttacccc tctagcgagc aggccttgac ccttcatgct
    72781 gtttttaaag atgggcatga agtagcagga gaaagttcta ttgccaaata cccaggcatg
    72841 attgaccatg tctatgtgac caacacctac aacgaccaaa agcctcaggc cagtcgtaag
    72901 gtggttgagg ctattctaga aagtgacatg attgtcttgg gaccaggctc actgtttacc
    72961 tctattttac ctaatttggt cattccagaa attaaagaag ctttgcgcca gactaaagca
    73021 gaagtggtgt atatttgtaa tatcatgacc cagtatggtg aaacagaaca attttcagat
    73081 gcagaccatg tggctgtttt aaatcagcac ttaggaagag atttgattga tacggttttg
    73141 gtaaatgtgg ctaaggtgcc gcaggcttac atgaattcta acaaatttga tgaatacctc
    73201 gttcaggttg atcacgactt tgctggcctt tgcagggctg ctaagcgtgt gatttcgtct
    73261 tattttttac ggttggagaa tggcggagct tttcacgatg gtaacttagt ggtggaagaa
    73321 ttaatgaatt tagtgaggat agtcaagcaa tgagttttac aaccaaggtc aaagaggaat
    73381 taattcactt gtccacaggt gacaacaatg aattggccgc cattatcaaa ttatcaggca
    73441 gcttgggctt agctcaccaa agccttcatt tatccattac cactgaaaat gctaaaattg
    73501 cccgctacat ttattctctt attgaagacg cctatgttat cgtgccagaa atcaggtatc
    73561 atcaaaagac gaacctgcgc aaaaaccgtg tttacaccgt ctatgttgag caaggtgtgg
    73621 aaaccatttt agccgatttg aaattagcgg actccttttt tggtctggaa acaggcatcg
    73681 aacctcaggt tttatcagac gataatgctg gccgttctta tcttaaaggg gcttttctag
    73741 cggcaggtag catcagagat cctgagtcag gcaagtatca gttagaaatc tattcagttt
    73801 atcttgatca tgctcaagat ttagctcaac tcatgcaaaa atttatgcta gatgccaaaa
    73861 ccatcgagca taaaagcggt gcagttactt atttgcaaaa ggcagaagat atcatggact
    73921 ttttaattat aattggtgcc atgtcctgca aagaagactt tgaagccatc aaattacttc
    73981 gagaagctcg aaatgatatt aaccgtgcca ataatgcaga aacagctaat attgccaaaa
    74041 ctatcagtgc tagcatgaaa accattaata atattattaa aatcatggac acaattggtt
    74101 tagaaagttt accgattgaa ttgcaacagg tagcccagct tcgggttaag catccagact
    74161 attccattca acaagttgct gatgctcttg agttcccaat caccaagagc ggggtcaacc
    74221 atcgcttgcg taaaatcaat aaaattgcag atgacttata gaacattttt atcgtatgag
    74281 cctttgttca tacgattttt ttgtctaaaa gtcgttttcc attgtttttt tgaagtgtct
    74341 gatgttctgt gatatgataa aagggataat aacgtttgta aaggagactg ataatggcat
    74401 gtacaactat actcgtcggt aaaaaggcat cttatgatgg ctcaaccatg gttgctcgaa
    74461 cagaagattc tcaaaatggt gatttcacgc ctaaaaaaat gattgtggtg aaaccagaag
    74521 atcaaccgag acattaccgt tcggtgcaat cttcctttga aatggactta ccggacaatc
    74581 ccatgactta cacgtctgtt cccgatgcgc ttggtaaaga tggtatctgg gcagaagcag
    74641 gcgtgaacga ggctaatgtg gccatgtcag caactgaaac catcactacc aattctcgtg
    74701 ttttaggggc agatcctcta gtagcttctg gtattggtga ggaggacatg gtgaccttag
    74761 tgctccctta tatccgttct gcgcgtgaag gggttttgcg ccttggtgcc attttagaag
    74821 actatggcac ttacgagtca aatggtgtgg ctttttcaga tgaacatgac atttggtggc
    74881 tggaaacgat tggtggtcat cactggattg caagacgggt gccagatgat gcttatgtca
    74941 ccaatcccaa tcaatttggc attgatcatt ttgaatttaa caatcctgaa gattaccttt
    75001 gctcagctga tttaaaggac tttattgaca cgtatcactt agatttaacc tatagccatg
    75061 agcattttaa tccgcgctat gcctttggta gtcaacgtga caaagaccgt cagtataata
    75121 cgccacgtgc ttggatcatg caaaaattct taaacccaga aattgtgcaa gacccgcgca
    75181 gctttgccct tgcttggtgt caaaaacctt accgtaaaat aacagtagaa gatgtcaaat
    75241 atgtattgag tagtcactat caggacacgg gttatgaccc ttacggttca gaaggaactc
    75301 ctgttagtaa gaaagtcttt cgccctattg gcattaaccg taccagccaa accgctatct
    75361 tacacattcg tcctaataaa cctcaagaaa ttgccgctat tcaatggatg gcttatgggt
    75421 caatgccatt taataccatg gtgccttttt tcacacaggt gaagactatt ccagattatt
    75481 ttgccaacac ttacgaaaat gtgtttaccg acaatttcta ttggaccaac cgtctgattg
    75541 cggctttggc agatcctcac tacaatcatc atgaaactga tttggacaat tacttggaag
    75601 aaaccatggc aaaaggtcat gccatgttac atgctgtgga agcacagtta ttggctggtg
    75661 aaacagtcga tttggaagag gaaaatcaaa aaatgagtga ctatgtgcaa ggagaaacgc
    75721 agacgctgtt gaacaagatt ctctttgacg ctagcaatct aatgaccaat cgtttcagtt
    75781 tgagcgatta atagtgaagc taaggctgag caactgctta gcctttttta cgataacttt
    75841 ttaggaaaag taatcgcaaa aagacaaaaa cttggaaaaa aaatcaaagt atgctatact
    75901 taactggtca atttttaacc agttaataac aaaggaggaa tcatgaaaaa gaaaattctt
    75961 ttaatgatga gtttaatcag tgtctttttt gcttggcaac ttactcaggc aaaacaagtc
    76021 ttagcagagg gtaaagtgaa ggtggtgaca actttctatc ctgtttatga atttacaaaa
    76081 ggggttattg gtaatgatgg cgatgttttc atgcttatga aagcaggaac ggaacctcat
    76141 gattttgagc cttctacaaa agacattaaa aaaatccaag atgcagatgc atttgtttat
    76201 atggatgaca atatggaaac ttgggtttct gatgtgaaaa aatcattgac atctaaaaaa
    76261 gtgaccatcg tcaagggaac tggtaacatg ctcttggtag caggagctgg acatgaccat
    76321 caccatgagg atgctgacaa aaagcatgag cataataaac atagcgaaga aggacacaac
    76381 catgcttttg acccacacgt gtggttgtca ccataccgta gcattacagt cgttgaaaat
    76441 attcgcgaca gtctttcaaa agcttaccca gaaaaagcag agaacttcaa agccaatgcc
    76501 gctacttata ttgaaaaatt aaaagagctt gacaaagact atacggcagc actttcagat
    76561 gctaagcaaa agagctttgt gacacaacac gcagcttttg gttatatggc acttgactat
    76621 ggcttgaacc aaatttctat taatggtgtc acaccagatg cagaaccatc agcaaaacgt
    76681 attgctactt tgtcaaaata cgttaaaaaa tatggcatca aatacattta ttttgaggaa
    76741 aatgcgtcaa gtaaagtcgc aaaaacccta gctaaagaag caggagttaa agcggctgtg
    76801 cttagtccgc ttgaaggttt gactgaaaaa gagatgaaag ctggccaaga ttactttacg
    76861 gtcatgcgta aaaaccttga aaccttacgc ttaaccactg atgtggctgg taaagaaatt
    76921 cttccagaaa aagacacgac taagacagtt tacaatggtt atttcaaaga caaagaagtc
    76981 aaagatcgtc aattatctga ctggtcaggt agctggcaat ctgtttaccc ctatctacaa
    77041 aatggtactt tagaccaagt ttgggactac aaggctaaaa aatctaaagg taaaatgaca
    77101 gcagccgagt acaaagatta ctacactact ggttataaaa ctgacgtgga acaaatcaaa
    77161 atcaatggta agaaaaagac catgaccttt gttcgtaatg gtgaaaagaa aaccttcact
    77221 tacacatacg ccggcaaaga aatcttgacc tatccaaaag gaaatcgcgg ggttcgtttc
    77281 atgtttgaag ctaaagaagc agatgctggc gaattcaaat acgttcaatt cagtgaccat
    77341 gccattgctc ctgaaaaagc aaagcatttc cacctgtact ggggtggtga cagccaagaa
    77401 aaattacata aagagttaga acattggcca acttactacg gttcagactt atctggtcgt
    77461 gaaatcgccc aagaaatcaa tgctcattaa tcttaactca ctaagcaaat cttcttttaa
    77521 ggaggtttgc tttttctttg tggagtaaaa aaatttaata attctcaaca ttaatttgac
    77581 ggacacttat gattataata tactttaagt ggttataaca agttagaggt gaataagatg
    77641 ccaaaagaac aaccactcta tctacagatt gttgatgact tggaagtgaa aatcaaaaaa
    77701 agcatgacag aaaacgataa gttgttgtca gaacgagaat taagtgacct ttacggtgtt
    77761 agtcgtatca cgattagact gactcttaag gaattggaat tacgagactt aatttataaa
    77821 aagcagggca aggggactta tgtttcagga atcaaagaac ctgccactga tttatcaagt
    77881 acttacagct ttacagagca gatgaaaaaa atggggaaaa ctcctaaaac agagattctt
    77941 tcttttgaac agtatcaggt aacaccttac ttgtctggtc tcttagagct tgatcccgat
    78001 acagaagttt ttgaattaga acgcctaagg attgcagatg atatgccctt gatgtttgaa
    78061 cgatcctata ttcctgctca gccttttcag gggttaagca ttgcggactt aaagcgtaaa
    78121 gctttgtacg atatctttgc aaaggactat caagaaacaa tccgtcttgc agaagaagag
    78181 ttttatgcca gcattgcctt ggactatgaa gcaggtctct taggcattaa aaagggagat
    78241 cctgtcttac atattattcg taaaacttat aatgataaaa atatcttaat tgaattgact
    78301 ttcagcattg caagggcgga tcagtttagg taccgggtgc aacatcaccc taatggttaa
    78361 ggaattggag gttaggacat gtttacaaaa acgcaggaag ctttggaggc tcttggtgca
    78421 gctatcacta ccaaggaaat taaacaagaa ccccgtttat ggcaagaaac catgacgttt
    78481 tttgaagaaa caagagattc tctggattct tttttaaaga gagtttgtaa cagcgctaac
    78541 agaaataacg ttcatgtcat ctttacaggt gcgggtacat ctgagtacat cggtaatacc
    78601 atttgccctt acttgaagaa ggtggggaat cggcagcgct atctctttga gagcgttgct
    78661 tcaaccgatt tggttgcggc gccagattat tatcttgttg aggaagagac ggtactatta
    78721 gtctcctttg ctaggagtgg taatagtcca gaaagtgtag cggctgttaa cctggtcaat
    78781 caactagtgc caaatagtta tcatttaacc attacctgcg ccaaagatgg agaactggcg
    78841 aaaaaagcac agcaagatga gcgttcttac ctttatttga tgcctgaaga cgctaatgat
    78901 gctggttttg ccatgactgg tagttttacc tgtatgatgc tagcggctct tttgattttt
    78961 gatgatgcta acagtatagc tcagaaagaa gcttatgtga ctgatatgat tcttgctggg
    79021 caagctgtta ttgaccaaga aagacggcta caagagctag ctgatttagg atttgaacgt
    79081 ttagtttatc taggttctgc tggtttagcc aagttaaccc aagaagctca attaaaaatg
    79141 ttagaattga cagctggaca agttgcgacc ctctacgagt cctctatggg ctttaggcat
    79201 ggtccgaaat cttttattaa tgaccgtacc ttagtcattg gatttgtcaa taatgatgct
    79261 tacgtcagac agtacgattt ggacatgtta gaggaaatcc aagcagatgg cattgccctt
    79321 aaaatcttgg ctctattgca aggaggggat atcaactttt cagaggacca atttcgacta
    79381 gacacccgtc acttattgcc agacgcttac ttagcttttc ccatgatttt agtcgcgcag
    79441 accttggccc tcttaacggc cgttacatta ggaaattccc ctgacacccc atctgcgaca
    79501 ggaacggtta atcgtgtggt caaaggagta acgattcacc cttacccgac tagtagtcgt
    79561 caatgcaaca aagcttaaag cttttgtgta gaagccgtaa aacgaaatga ttttgtgcta
    79621 gtaggtcaaa agaaaaaccc tcctgataca ggaaggtttt ttgtattgtc ttgcttattt
    79681 tgctgcgttt gcgtctttga gaccgtattt tttgttgaag cggtccacac gtccatctgc
    79741 ttgagtgaac ttttgacgtc ctgtgtagaa tgggtgtgaa tctgatgaaa tttccacacg
    79801 gataagtggg taagtttcac cttcaaactc aacagtttct ttagaagctt ttgttgagcc
    79861 gctaaggaac tggtaacctg tagttgtatc taggaaaaca actggacgat aatctggatg
    79921 aatgtctttt ctcatcttaa aaaattcctt tctgccatga cacttaatgc atcatagtta
    79981 ttaaccattc tagtgtaaca aaacttgaat gacttgacaa ggcttttctt tccttatttt
    80041 ttaaaaatcc taaatctctt ggcaaacagc gatgagttct cggaaaatag cttgattttc
    80101 ttctaaatta gctgagttgg ctccgctagc aaggggatgc ccccctccac cgtgacgctt
    80161 agcaatgcca ttaataatct tttctttact gcgcatacgc acacggtagt tgccgtcagt
    80221 taactcaaca aaaatagccc aagcttgaac gttatcaatt ttaccaggag cacagacaat
    80281 ggcagagctt tctgctaggg tcacgtcaaa atgttttaag gtttcttggc tgacaaggac
    80341 ataagcagcc ccactctcat caattgttaa atgctcaaag acgtagcttt gcagctttgc
    80401 tattttcaaa ggaaacgaat ccatttgcct tgaaatcgca gcaaagtcga actcgaaatg
    80461 tctgagttgg ctagcaatgg aaagggtttt actagtggtt gaggcataaa gaaatcgccc
    80521 tgtatcacca acgataccgg tgtataagag cttagcagcc ttgtcagaga gagtaagatt
    80581 ctgactaaag gcaaagtctg caatgatttc acttgcgcta gaagcgcttg tgtccacata
    80641 atagaagtca ccatacacat catcgttggg atggtgatca atcttaatta agcacttccc
    80701 cagtgtgtag cgctcatcat caatccttgg tcgattcgct gtatctgtaa tgatgaccaa
    80761 agcctcttta tagtctttgt cagtcacctg atccatttgg ctaatccaag ctaaactagg
    80821 ctcatcaaaa ccagtcatca aaaccttttt gtctgggaaa ttttgtgcaa taatttcttt
    80881 caagccggcc tgactaccaa gagcatcagg gtcaggattt tgatggcgat ggataataat
    80941 agtttggtga gcttttattt tatctaaaat tgtttcaaaa gttgttatca tgctttaatc
    81001 tttctttttc aaaaagagga aaataaccgt ttaacatagt cttcaaaaaa cggtttgtgt
    81061 catagggagg tgtgcgttat gttaagtggc taatgctaga attttttaag attgtcaaag
    81121 accataaatt gacattttat ctgaaaatca tatcctattt tattatagca taaaagtaag
    81181 ctctagataa tggtaaatta atctaggaag aaaagaggtt agttatcagt tatgatacta
    81241 tcaaagctag tcttgcaaat gcaagtgaaa aaatagtttt taagtgtttt aagcaatcat
    81301 atcaaccttc tcgttaaaca attagtgctt ttcctaaaaa agtgtgctaa aataaacata
    81361 gtttagtaat tttgggagga atttatggca ttagcaaaaa tcgtttatgc cagcatgacg
    81421 ggaaatactg aagaaatcgc tgatatcgtt gcaaataaat tgcaagaatt gggccatgat
    81481 gttgacattg atgagtgcac aacagttgac gcttcagagt ttgagaatgc tgatatcgct
    81541 gtcgttgcga cttatactta tggtgatggt gacttaccag atgaaatcgt tgatttttac
    81601 gaagatttac aagatttaga tttagaaggt aaaatctatg gcgttgtcgg ctctggtgac
    81661 actttttatg actatttttg caaatctgtt gatgactttt cagagcaatt tgcgcttact
    81721 ggtgccatca aaggtgcaga gcctgtcaaa gtagacttgg cagcagaaga tgaagacatc
    81781 gatcgtttag aggcctttgc agagcaactc tcacaggctg ttaataatct ataaaactat
    81841 ttagtaaaaa tgtcagggac ttccttggca tttttagatt tttaacctaa aataaaggga
    81901 atgtcacttc agggtaggaa ttgaaaaaaa caatcagctt caccttgctt ctgatcctct
    81961 tattaactat aataaaaaga taatctaata catctagaaa aggaaagtta tgcgtttaga
    82021 aaagatacga caagaaatca atggtattga tcaccacttg gttgccttat tagaaaagcg
    82081 gatggcactt gtagagcaag taacagctta taaactagcc aatcacttgc ctgtcttaga
    82141 tcaggcgagg gaaaatcaga tcctagatag ggtttcttat ctcgttaaag accaagcatt
    82201 tgagccagct atccatgaga cgtttaaaac cattatgtct ctttcacggc agtatcaaac
    82261 gcaacactta acaggtggtg acattaatga ttaaatcaat tgcaacactg cctaaacctt
    82321 ccttgaaaga agtgttagga ctagtaggat tagcacttcc catagggggc gcggttggag
    82381 tggttgatgt tatttttggc aaagggcttt tgtttctttc cgaatatcgc gatcaccacc
    82441 ttttcttgct tcttccattt cttgccctag caggccttgt tatcgttttt ctttacgata
    82501 agttaggcaa agaggttcgt caagggatgg gattggtttt tcaggtaggg catggtcaaa
    82561 aaaatcagat tcctcctatt ctcattcctc ttattttgtt ctcaacttgg gtaacccact
    82621 tatttggagc gagtgcgggt agagaagggg tagccgttca aataggagct actatctctc
    82681 attattgtcg gagatttgtg actagtcaag aagccgcacg acatcttttg attatgggga
    82741 tggcagccgg ttttgcaggg ctttttcaaa cgccaattgc tgctgtcgta tttgccctcg
    82801 aggttttatt agtaggaacg cttcgttatt ccgctttatt gccttcctta gttgctgctt
    82861 acgtggcttc ttggacttcc catgccttgg gattagagaa atttacgatt gttcttgaag
    82921 aaacattgac cataactccc ttaacattgg ttaaattaat tgggttaggc cttatttttg
    82981 gtctggtggg aaatagtttt gcctatttac ttggctggtt taaaccttac ctcagtcaaa
    83041 agctaccaaa tccttatttc cggattgctt ttatcggtgc tttgttatct atctgtctga
    83101 tgattggtca cgtcggaaga tatagtggtc taggaaccaa tttgattgcg gcagcttttt
    83161 ctggccaaac tatcctaact tacgactggc ttttaaaaat gatcgtgacg gtgattagct
    83221 tatctgcagg ctttcaaggt ggtgaagtga cgcctctatt tgctattgga gcttctttag
    83281 gcatcgtttt ggccccttac ttaggtcttc ctgtactgtt ggttgctgca cttggttaca
    83341 ccacggtatt tggaagtgca acaaatacct tttgggcccc tatctttatt gggatagagg
    83401 tctttggtcc agagaatgcc ctagcatatt ttgtcacctc ggctgcagcc tatatggtta
    83461 gtcacagaca ttccatttac agttatcaaa aagtagtcat gatcaattta aaagacatga
    83521 cggtaaatca gatggattta aaaaaatagc tttacaataa agaagctttt tgttagaata
    83581 aatctcgtgt gtaatggaca cacgaaaatt acggctaatc cgctaagaca agtacttaag
    83641 attagtaaga taggagaata aaaatgaatc cattaatcca aagtttgaca gaaggtcaac
    83701 ttcgctctga tatccctaac ttccgtcctg gtgacactgt tcgcgttcac gcaaaagttg
    83761 tcgaaggaac tcgcgaacgt atccagatct ttgaaggtgt tgttatttca cgtaaaggtc
    83821 aagggatctc agaaatgtac actgttcgta aaatctcagg tggtatcggg gtagagcgta
    83881 cattcccaat ccacacacca cgtgttgaca aaatcgaagt tatccgccac ggtaaagtcc
    83941 gtcgtgcgaa actttactac ttgcgtgcat tacaaggtaa agcagctcgt atcaaagaaa
    84001 ttcgtcgtta gtttgaaccg aaacgtaacg taatagaata aaaagactag agtctctagt
    84061 ctttttatgt cgtcgtgaca atgtgacaat tggtgattaa cgctatgctt gtaggacact
    84121 ttgaaagttc cttagcagtt ataattagta tattgtaata acaaaatgaa gcaaaataaa
    84181 ctttcagtta tctgatattt catcttgcat taccttattt tctaggttaa aatagaagac
    84241 aaggtgagta taagatagac cccttagttc aatggatata acaactccct cctaaggagt
    84301 agttgctggt tcgattccgg caggggtcat tattgtcact gaaaaagcct ttacatcaaa
    84361 ggctttttgt tttattccgg ttttaaaagg gtcagtttgt ttatttataa tttctttcat
    84421 gtttacagtt gtgtgactgt aaatagataa tgttgttttt ggatcgctgt gaccaactct
    84481 atccattatc gcatttagcg gtatcccttt ttctgctaaa aatgatatat gcgagtgtct
    84541 aaataagtgc gtgtgataat ctccgtaaat tttcaatcgc ttattgatgt acgcgtttaa
    84601 aattggtaaa ccttttgaat atggaaagct gttagcactc tgcttgtgat taatctatcg
    84661 tatgttaaaa gtgacattta aattaaatca ttttttatac atatatttca aaaggattta
    84721 tcatattgga aaatagcggt gcttctattg gcattttact attgacagga atttccctgc
    84781 cttttgtctc ctaaagaaga tcagctttga tttctaactt gattagagtg ggcttggtac
    84841 tgtttatgaa ttatcagtac atactttctg gagatcaaaa aggaaaggaa cagctaaaga
    84901 ggtgtatgtg ttaccattag aggaatagaa catcgcaaga ctcgtttatt cagtcattac
    84961 gatacctgtt ttatttgtga aataactata atattaagtg aatttgtgat ataatgaaag
    85021 gtatgaatta tcaagattat atatgggatt tgggaggtac cttactcgat aattacgagc
    85081 tttcaaccca agcttttgtc caaacgctag cttttttcag ccttccagga gatcacgatg
    85141 ctgtttacca aaaattaaaa gaatcaaccg ctatcgctgt ggcaatgttt gctcctaatg
    85201 agccagaatt tttgcatgtg tataggctaa gagaagcaga caaattggca caacccattt
    85261 ggtgtttggg agctaaagaa attttgggaa agatagcgac ttcaggttcg cgaaattttt
    85321 taatttctca tcgagattgt caggtgaacc aacttttaga gcaagctggg ttgctaatct
    85381 attttacaga agtcgtgaca gcttcaaatg gctttgctcg gaaaccaaat cctgaaagtt
    85441 tgttttattt aaaagaaaag tatgatatta atagtggatt agtaattgga gatcggctaa
    85501 ttgataaaca agcaggacaa gcagcgggtt ttaatacctt gcttgttgac ggtagaaaaa
    85561 acctattgga gatagtaacg tagatgattg aagaaaataa acattttgaa aaaaaaatgc
    85621 aagaatacga tgccagtcaa attcaggttc tagaagggct ggaggctgtc cgcatgcgtc
    85681 cagggatgta tattggctcg acagctaaag agggtttgca tcatttagtc tgggaaattg
    85741 ttgacaactc aattgacgaa gcattagcag gttttgcctc tcatattaaa gtctttattg
    85801 aagcagataa ttccattaca gtagtagatg atggccgtgg aattccagtt gatatccaag
    85861 ccaagacagg acgtcccgcc gttgaaacag tttttacagt cttacacgca ggtggtaaat
    85921 ttggtggagg cggctataag gtttctggag gattacatgg tgtagggtca tctgttgtta
    85981 atgctttatc aacacaatta gatgtacgtg tttataaaaa cggccaaatt cattaccaag
    86041 aatttaaacg cggggctgtt gtagcagatc ttgaggtcat tggaaccact gatgtgactg
    86101 gcacgaccgt acactttaca cccgatccag aaatttttac cgaaacgact cagtttgatt
    86161 acagtgtttt agcaaaacgt attcaagagt tagccttttt gaatcgtggt ttaaaaattt
    86221 ccattacaga taagcgctca ggtatggaac aagaagaaca tttcctttat gaaggtggaa
    86281 ttggttctta tgttgaattt ttaaatgata aaaaagatgt tatctttgaa acgcccatct
    86341 atacagatgg tgaattagaa ggtattgcag ttgaagtagc catgcaatac acgactagct
    86401 atcaagaaac agtcatgagt tttgctaata atattcatac tcatgaaggt ggaacgcatg
    86461 aacaaggctt tagagcggct cttactcggg tcatcaatga ctacgctaag aaaaataaaa
    86521 ttcttaaaga aaatgaggac aatttgacag gagaagatgt tcgtgaaggt ttgacggcgg
    86581 taatttctgt taagcatcca aatcctcaat ttgaaggtca aaccaaaaca aaattgggca
    86641 actcagaagt ggttaagatc actaatcgtc tctttagtga ggcctttcaa cgttttcttt
    86701 tggaaaaccc acaagttgct cgtaagattg tggaaaaagg gattttggct tctaaagcta
    86761 gaattgcagc taagcgagcc cgcgaagtca cccgcaaaaa atcaggctta gaaatttcaa
    86821 acttacctgg aaaattagca gactgttcgt caaatgacgc taaccaaaac gaacttttca
    86881 tcgtcgaagg agattcagcg ggtgggtcgg ccaaatcagg tcgtaaccga gagtttcaag
    86941 ctatcttgcc tattcgcggt aaaattttga acgtggaaaa agcaactatg gataagattc
    87001 ttgccaacga agaaattaga agtctcttta ccgctatggg tacaggtttt ggtgcagatt
    87061 ttgacgtgtc aaaagctcgc taccaaaagc tggttatcat gaccgatgcc gatgtggatg
    87121 gcgctcatat tagaacctta cttttaacct tgatttaccg ctttatgaga cctgttctag
    87181 aagctggcta tgtttacatc gcccagccac ctatttatgg tgttaaggtc ggtagtgaga
    87241 ttaaagagta tattcagcca ggtattgatc aagaagacca attaaaaaca gctcttgaaa
    87301 aatatagtat tggtcgttca aaaccaactg ttcaacgtta taaaggtctt ggggaaatgg
    87361 atgaccatca actttgggaa actactatgg atcctgaaaa tcgtttgatg gcgcgtgtga
    87421 cagttgatga tgccgcagaa gcagataaag tatttgatat gttaatggga gatcgtgttg
    87481 aaccaagacg tgatttcatt gaggaaaatg cggtttatag tacactggat atttagtcac
    87541 ctttttagtt tcacaaggaa tctgctacca aagaggacga attgactaaa aatcatgatg
    87601 catgatgcca acagctttgt tagttagtac gtcttcatct tgagggacag cgttaatgca
    87661 taggtattga gaccgcccct taatacctat gttagtcctt attaagaaag ccttttgagt
    87721 cgctggtacg agattgttga attagtcaaa tatattttga aatgcatttt aaagtatgtt
    87781 ataatcgtaa tgttatagtg tattttaaat aaaattttaa taaaaggcga ttgaggaatt
    87841 tcagttttta gttattactg gaacaatgtt gataaatcgt gtctaaataa taaggagatt
    87901 cagatgtcaa gcggaattat cttgctgatt gtggctatag tcctactagt gattatcgcc
    87961 tatctagtgg gtgtcatcat acgaaagcgt aatgattccc taattaccag tctagaagaa
    88021 cgaaaacagg ccttgtttgc tttgcctgtt aatgatgaaa ttgaagaagt aaagtcactt
    88081 catttgatag gacagagcca aacctctttt cgggaatgga atcagaaatg ggtagactta
    88141 actgtgaatt cgtttgcaga tattgaaaat catatttttg aagctgaaaa tttgaatgat
    88201 acgtttaatt ttattcgcgc caagcatgag attaatagcg ttgaaagtca gcttaatctt
    88261 gttgaagaag atatcgcttc tattcgtgaa gcacttaata tcttaaaaga gcaagaagaa
    88321 aaaaatagtg ctcgtgtcac ccatgctctt gatttatatg aaaagctcca agcttctatt
    88381 tctgagaatg aagataattt tggttcaact atgcctgaaa ttgacaaaca gatgaaaaac
    88441 attgaaactg aattttcaca gtttgtcgca ctcaactcat caggtgatcc tgtagaagct
    88501 tctgaagttc tggatagagc agaagagcat actattgctc tagggcagat tacagagcag
    88561 attccagcta ttgtggccaa gttggaagat gattttccag atcagttaga tgatttggaa
    88621 acaggatatc gccgtttact tgaagaaaac tatcattttc cagaaaaaaa cattgaggca
    88681 cgttttcagg agattcgtga gtctattcgt gccaattcat cagagttagt tacacttgac
    88741 ttggatcgtg ctagagaaga aaacacgcat attcaagaac gcattgattc tctttatgaa
    88801 gtctttgagc gcgaaattgc cgcttataag gtagctgcta aaaatagtaa gatgctacct
    88861 cgttacctag aacatgtgaa acgtaataat gaacaactca aagatgaaat tgcacgttta
    88921 tcacgtaaat atattctaag tgaaacggaa agtctgactg ttaaggcatt tgagaaggat
    88981 attaaagaga tagaagacag tacacttgct gttgcagaac aatttggttt acaagaaaaa
    89041 ccattttcag aactgcaagt gacttttgaa cgcagtataa agacgcttac taatgtcgag
    89101 tcaggtcaaa tggatgtttt tgcagcagtt aaagatattg aaaaaattga atcacaggca
    89161 cgccataacc ttgatgttta tgttactcag cttcatatga ttaaacgtta tatggaaaaa
    89221 cgtcacttgc ctggaattcc acaagacttt cttagtgctt tctttacaac gagttcacaa
    89281 ttggaagctt tgatggatga gcttagtaga ggacggatca atattgaggc agtgtcacgt
    89341 ttgtcggaag ttgcgacggt tgctattgct aatctagaag atttaaccta tcaagtggtt
    89401 caaaatgcta ccttgactga acaattacta caatattcta atcgctatcg ttcttttgaa
    89461 gcaggagttc aaagtagttt tgaacatgct ttgagacttt tcgaagtaga aaatgattat
    89521 caggcttctt ttgatgaaat ttcttatgct cttgaaacag ttgaacctgg ggtaacagat
    89581 cggtttgtta actcttatga aaaaacacga gagcacattc gtttttgata cttgaaagtc
    89641 gtgatattct cacgactttt tattagcttt ttggaccaat acgtgttttt gactagaatc
    89701 ttctttttaa tgatcaaacg aagaagtagt aacagtgttt tttgctattg agaggatagt
    89761 tattttttca aaaatctttt ccaaaaggat ttggtttttt caggagtttg tttgctaagt
    89821 tgatcgtttt ttgtctgctt taagatgact tctaaacgtg tttccttcag atttattgcg
    89881 tggtcggtat gaaggataaa cgcaaaaggt gaagtcatat gcgtttctgt aataatagta
    89941 acaggtaaat ggttttcttt tgctaatttc atatagagta tttgaagttg aggtgccagc
    90001 ttggaagaga tttttaagtg taaaggttga tagactaact gaaggctcgg taattctctt
    90061 ttcaaatagt cttttactgt tttagtttga gcttcgtcta ataaagcact taaaacgact
    90121 cgttcagcat acgttcctag atagtagcgt tgctgctcag gatttaatct tctttctccc
    90181 caggctcctt ttaatagctt atcttctaaa ttagtcatag ataccctcgc ttctttataa
    90241 tagtttcttt attatatcga tttctacaat aaaataccaa tttgaaaatt ttcggtaaat
    90301 gtgttaaaaa acaagaaaga aagtgatttt tcttgcaaaa aacaaaggaa gcttttcatg
    90361 agattgcttt ttaagttgat taaaggccta gtttgtggta ttatgaacat gtaaaatatt
    90421 aaaactcata aggagagtat tacaatgtca attattactg atgtatacgc tcgcgaagtc
    90481 cttgactcac gcggtaaccc aacacttgaa gtagaagttt atacagaatc aggtgcattc
    90541 ggacgtggta tggttccttc aggagcttca actggtgaac atgaagccgt tgaacttcgt
    90601 gatggtgaca aatctcgtta ccttggtcta ggtactcaaa aagcagttga taacgttaat
    90661 aacattatcg ctgaggcaat catcggttac gatgttcgcg atcaacaagc tatcgaccgt
    90721 gcaatgatcg ctcttgacgg tactcctaac aaaggcaaac ttggtgctaa tgctattctt
    90781 ggtgtttcta tcgctgttgc tcgtgcagct gctgactatc ttgaagtgcc actttacact
    90841 taccttggcg gattcaacac taaagttctt ccaactccta tgatgaacat catcaacggt
    90901 ggatcacact cagatgcccc aattgcattc caagagttca tgatcatgcc agttggtgca
    90961 cctactttca aagaaggtct tcgttggggt gctgaagttt tccacgctct taagaaaatt
    91021 cttaaagaac gcggacttgt tacagctgta ggtgacgaag gtggatttgc tcctaaattt
    91081 gaaggaactg aagacggtgt agaaactatc cttaaagcta ttgaagcagc tggttacgaa
    91141 gctggtgaaa acggcattat gatcggtttt gactgtgcat catcagaatt ctacgacaaa
    91201 gaacgtaaag tttacgacta tactaaattt gaaggtgaag gcgctgctgt tcgtacatct
    91261 gcagaacaag ttgattacct tgaagagttg gttaacaaat acccaattat tactatcgaa
    91321 gatggtatgg atgaaaacga ctgggatggt tggaaagttc ttactgaacg cctaggcaaa
    91381 cgtgttcaat tggttggtga cgacttcttc gttacaaaca ctgaatacct tgctcgtggt
    91441 atcaaagaaa atgcagctaa ctcaatcctt atcaaagtta accaaatcgg tactttgact
    91501 gaaacgtttg aagctatcga aatggctaaa gaagctggat atactgccgt tgtatcacac
    91561 cgttcaggtg aaactgaaga ttcaacaatc gctgacatcg cagttgcaac aaacgctggc
    91621 caaatcaaaa caggttcatt gtcacgtaca gaccgtattg ctaaatacaa ccaattactt
    91681 cgtatcgaag atcaacttgg tgaagttgct caatacaaag gtatcaaatc attctataac
    91741 ttaaaaaaat agacggtaag acagaaatcg gtcattcgta gaatgcgatt tcgtcgtcgc
    91801 acctccgtca ggctgactag gttggtcacg acaaaaggag tgaacatcca gtcagctacg
    91861 acgcaaaagt taaagattaa agaatagaaa gcttgggaaa atcccaagct ttttgctttc
    91921 taaatagtaa aaatttactg tgggctaatt tatcctgagc taatgtatta gcgaggtaaa
    91981 gttactgaag aagacttgga tttgtactgc ctctaaaaag ttggacacta tattttaaat
    92041 acaggactaa gtccttttat accaattgtc aagcacatca aggttttaac tgaaaaaatg
    92101 ttttagacaa tagcctaggt ttagctgttg tcttacttgt tgtgctctga ttagcataaa
    92161 aaagtgtaca agaaaaaaac cttgcctagt atttttaaga tcaaggtgtt acaatgatag
    92221 agcaacaacg actttaatga ttttgggtcg aagcgtaatt gtaaagtttg ttatgcgtta
    92281 tgaggtaaag cattgtctga caaggcaatg tctagaggca atcgtatgtg gcttggttga
    92341 agctgtttgc gattgccttt ttcgtttctc atagaagtct gcgatatgac aaggattggt
    92401 atgactagca aaagcaatgt catggataca cttgaaaaga atctttggag cataaggatt
    92461 cccacgtttg gtaatgtgct cttgagctag atagttgcca gactcataat gtctaaagtc
    92521 aatcccaatg aaagcattaa tttgattagc agattgaaaa cgacgaatag caccgagttc
    92581 gccaataata cttgttgcag ttgtttctac gatacttgga atagagcgta gaatcttgtc
    92641 ttctggtaaa ggttgagccg acgccaccat cttatcaaaa atagcttgac gacgttcaga
    92701 aagtctaagt aattctttgg catagtagcg aacctcttct atcatcggag aggttttcgt
    92761 aacagtaaaa taagactgct tggctaatga gacaagtttt tccactaagt agatcccacg
    92821 cttgtcagag atacgtttag aggtagattg acggatgata gctgacaatg ctttgtcagc
    92881 tagctccagt acaaagctat tacatgaaaa aagccgttac cacattccag tattactcac
    92941 cagttggtgc aaataagatg tgttccagtt caggaaaagt gacttgtaag atcttgtgaa
    93001 ggcgattctt aactctcgaa caatattatc ctcagtcaag ttttgataga agcgactcaa
    93061 gtcacgtagc agctgataaa cttcttcctg aacgtaagtt ggttttctaa ctagaataaa
    93121 ttgagactga gccaattttt cagtgtctaa cttatccgct ttacgcaccc gtagactatc
    93181 tagttttcta aaggattgag ttgtgtataa gcgtagccat ttttctcaag gaaggactga
    93241 agccgacgag aatagactcc tgttgtgatg gtctcccttt tgccttttaa acaatagtat
    93301 agccgttttt caaaatgtta ttaatgattt tttcctttat tccagaagaa taagtctgat
    93361 actgtttctt attaataatc tcaatgccct aacaatccat cagtcgaacg gtatttctca
    93421 aattactgca gttgactctt gcgttcagtt ccctcacctt gtggatgccg tttagaacaa
    93481 tattagcttg ctgagccttt tggtaatgtg aacaaagcat ctttatcact caaggagaag
    93541 acaggttctt gtggtaagtg tccaacaagg tcacgataaa ctcatcgtac cgtttttggc
    93601 ggaagtctag aaatgcctta tgaatgtaag cctcagaaac gtctctaaaa ctaaacaaga
    93661 aatacaagaa caaatgccct tgattctggt acaaatgctg tatcaggttc aggtattgcc
    93721 tatctgcgat aatgacactc tgcttttcct taacaatatc tttgaaagga atgatttcga
    93781 cattgacagg cataaccgtt gcaatagctg gcgggaagta aattgagagt gcattggtca
    93841 tcaaatccag cgcagcagtg ttattggtca aaagatagac aggtatcggt ggcaaatatg
    93901 tttttaggag ttcctctaac tgtatcgtga agagcaataa atgataagac ggcagtttcc
    93961 ccacgatgcc ttctgcgctc atccattcct caacgatggt ttttagagta ttgtagaagc
    94021 tcttgtttcg tttttgctgt cttctgaggt aatggaacaa gtgtatgtca ggtaacaaaa
    94081 cttgaagatt actgataaat agacgtgaga aggaaaccaa aatccgtttg aaaggaaggg
    94141 agtttaggat attattgcct agtagtggac gcagggatgc tttcaaggtt tttccatttg
    94201 ttttgtcatc accaactcaa acaattcttt cactttttta ggtgtccatt tatccttatg
    94261 gaaaggatta ttggtggtgc aaaaacataa aaaaatataa tccaaatcct catcagttaa
    94321 ctcaggatgg taccattttc cgatgatttc ctgactggtc attttgatat ctcgatagat
    94381 agacagtttt ttcaagtgtt tgaatgcatc tgtctgaggg atccccaagt tatcatgtcg
    94441 ccgtttccat ataagggata tcaaaatggc aaagtatgaa aattcataag gggctttact
    94501 ggcatcatta agtgtggtgg cttgattgga gtttataatc agcgaaacca ctttattcat
    94561 tgatgtttta tcaaaatcat aaatggtcat accaaactga tagtgcaaca aggcgatcag
    94621 aaagcggata cggtattctg gtccaacaac atggttttgc cgaacgttta aacctacctt
    94681 tttcaagtat ttccgacaat tggtcctgat acggtaagcg gtggcttggg aaatatactc
    94741 tcgtttaatg aagtcgtgta ggctcagctc tcctggttca attaaaaaac ataacatctt
    94801 taagaatatc gaggtttgat agagtttctt aaaataaggc attttaagtt ggtaggtctt
    94861 ataggggata gaaacagtgt cgtcacaaat ggttaaggtg agggtgtctt ctaaatcctt
    94921 aatcatttca aagagtaagt tgtagacacg ttgttttttg agttcggttt cctgacacaa
    94981 ctggtctatg gagacttcgg aaacgtctaa caaaatgttg agcagtgata atttatcttg
    95041 taatttggtt tctagatgaa ggtgtaacat aactgaactc ctttcatttt tatagcagaa
    95101 actcgtattt atttaattat cacataatta aataaaacag tcaaataatt caatggtgac
    95161 gcgtgtttaa ggagtattga gaatgtcaga tataagcaaa atgcagctga atattcttaa
    95221 ttttaggtat gaaaaagcgt cgggattgat aaaaaatcta ttgaattggg taaatttaag
    95281 ttgatataat aagttgatta gaaaaattaa caattcattt aaattctaat cagctgtaac
    95341 gttccggggc gtgttaaact gtctgaacat agaaaataca agtttctacg gtgactaaga
    95401 taagttaagg aggattcctt aaattctgtg ataggaacgt aaactctcta caagagtaga
    95461 gcacgtcatc agtcagtctg ggtgtctact ggctgacaac gtcaaataag ataggcatga
    95521 aaaaataagg gggaatacag aatgttttta aaacaccaag atgtgaaaca aaaaaattgg
    95581 agaatgcgta aggtcaaaaa agtctttgtt agttcatgta tgcttttaac agtgggcctc
    95641 ggagttgccg tacctactgg attcagccaa tctaatggcg tgatggttgt aaaggctgcg
    95701 gaagtgccgg cgacagattt atcacgtcag gcgtctgatt cggagagggt agatgaatcg
    95761 tctttattgc agaaagaaaa cttatcagta gattcattta aattagaaaa tttaaatgga
    95821 tgggaagctg aaaatgatac agcaggtaat ttggggaaat ttaaagatcc agatagttcg
    95881 ggctatcaaa atattttgac atcatctgga aagaatatca gtgtagctgt tgctcccaaa
    95941 ggttcaggta aaatgaacat taaagtaact aaaagatcaa attttcaggg tggatattat
    96001 gtaggtggtc ttagaactca aactccggta ttgaagttaa atgatgttta tcgatattct
    96061 tttacaacta aaaaattatc aggaaattct tcagagttca aaacgagagt taagcccgtt
    96121 gaatctaata ataaactagg gaaagagctt gttattaggg tggataataa aaatgtatct
    96181 actaagcatg attggcttcc agacatctct gatggaactc atactgtgga cttcactggt
    96241 cttgataaaa aattatctgt tgctttcaga ttttctccaa gacaaacttc gaatgttgtt
    96301 tacgaatttt ctaacataaa tataaaaaac attagtcctg catcagtgcc ggctattcct
    96361 tcgaaagttt tagagggaac cagcgtcttg tcgggtactg caatatcttc tggagataca
    96421 ttagaaaaaa gaaaatcgtt tgatggcgat atcctaagag tttataaaga tagcaaaatc
    96481 attgctagaa cagtaataaa aggcaataag tgggatgtta aactttcaaa gcctcttatt
    96541 gcaggtgaaa aattagattt tgagattttg catccgagat ctcaaaacgt tagtaaaaaa
    96601 atttcaaaac aagtcgaagc taaaccattt gatccagctt cctataaaga aaaagttata
    96661 gccaaattaa agccggttta tgaagctact agtgaaaaaa tcacaaatga tgcttggttg
    96721 gatgaaaatg cgaaggattt gcaaaaacaa aaattagaag aacaatatat ttctggaaaa
    96781 gtagcgatat cagaggctgg aactaaacaa gaagctatag atgcagcata taataaatat
    96841 tcaagtcaaa cagatccaga ctctcttcct agtcagtata aacaaggtaa taaagaaaat
    96901 gaacaagaaa aagggcgtca agatttaatc cagactcgtg atctgacgtt gaaagccatt
    96961 caagaagaca aatggctaac agagcaggag aaaacaattc aaaaagaaga agcattaaaa
    97021 gcttttgaaa ctggtataga aagtgttaat caaacagtat cattagaaca gttgaagcaa
    97081 cggttaatag tgtataaagc ttctgaaaaa gattcagaga aaaaagaata tcctgagtca
    97141 attcctaatc agcatattcc agggaaagaa aaagaagtta aagctgctaa acaagaagaa
    97201 cttaaaaaat tacatgacac aactcttgaa aaaatcaatc aagataaatg gttgacgcca
    97261 gaccaacaag ctgaacagtt aaaacaagcg gaagttactt ttaaaaaagg ccaagaagca
    97321 attaaaagtg ctcagacttt aactcagctt gagacagact tagctgatta tgtttctgag
    97381 aatgaaggta agggaaattc tattcccgat aaatacaaat ctggccataa agatgatttg
    97441 gtaaataagg ctgaagttaa acttaaggaa gctcacgaag ctactaaaca agcaattgaa
    97501 aaagatccat ggttgagtcc ggaacagaaa aaagctcaaa aagaaaaagc caaagcaaga
    97561 ctagatgagg gcttgaaagc tcttaaagct gcagatagtt tagagattct taaagtgaca
    97621 gaagaagctt tcgttgataa agaaaaaaat ccagattcaa ttccaaatca acataaagct
    97681 ggaactgctg atcaagctag aaaacaagct ttagatagtt tagataagga ggttcaaaaa
    97741 gagttagagt caattgataa cgataatact ctaacaactg atgagaaagc agctgctaag
    97801 aaaaaagtca atgacgctta tgatgtagct aagcaaacag ctatggaagc caattcttat
    97861 gaagatttga ctactattaa agatgagttc ttatctaatt tacctcataa acaaggaacg
    97921 ccgcttaaag atcaacaatc tgatgctatt gcagaattag agaagaagca gcaagaaatt
    97981 gaaaaagcta ttgagggtga taaaacatta ccaagagacg aaaaagagaa acaaattgct
    98041 gactctaagg aacgcttaaa atctgacacg caaaaagtta aagatgctaa aaatgctgat
    98101 gctattaaaa aagcatttga agaagggaaa gtgaatattc ctcaagcaca tatcccaggt
    98161 gatttgaaca aggataaaga aaaacttctt gcagaattga agcaaaaagc agatgatact
    98221 gaaaaagcta ttgatgttga taaaactctg acagaagatg agaaaaaaga gcaaaaagtc
    98281 aaaacaaaag ctgaacttga aaaagctaaa actgatgtta aaaatactca gacacgtgaa
    98341 gaactagata aaaaagttcc agaacttaag aaagctattg aagacactca cgttaaaggt
    98401 aatcttgaag gtgttaagaa taaggctatt gaagatctta aaaaagctca tactgaaaca
    98461 gttgctaaaa taaatggtga tgataccctt gacaaagcta ctaaagaagc tcaagtgaaa
    98521 gaagctgaca aagctttggc agcaggtaaa gatgcgatca ctaaagcaga tgatgctgat
    98581 aaagtaagta cagctgttac agagcacaca ccaaaaatta aagcagcaca taaaactggt
    98641 gaccttaaaa aagctcaagt agatgctaac acagctcttg acaaagcagc tgaaaaagaa
    98701 cgtggagaaa tcaataaaga tgctacacta acgacagaag ataaagcaaa acaactgaaa
    98761 gaagttgaga cagctcttac taaagctaaa gataacgtga aagctgctaa gacagcagac
    98821 gctatcaatg acgcacgtga taaaggcgta gcaacaattg atgccgtcca taaagcaggt
    98881 caagacttag gtgctcgtaa gtcaggtcaa gtcgctaaac ttgaagaagc agctaaagca
    98941 acgaaagaca agatttcagc tgatccaact ttgacaagca aagaaaaaga agagcaatct
    99001 aaagctgttg acgctgaact taagaaagcg atagaagctg ttaacgcagc tgacacagct
    99061 gacaaggttg acgatgctct tggtgaaggt gttacagaca tcaagaacca acacaagtca
    99121 ggtgactcta tcgacgctcg tcgtgaggct catggtaagg aacttgatag agtcgctcaa
    99181 gaaactaaag gtgcgattga aaaagaccct actttgacga ctgaagaaaa agctaaacaa
    99241 gttaaagacg tagatgccgc taaagaaaga ggcatggcta agcttaatga agctaaagat
    99301 gctgatgctt tagacaaagc ttacggtgaa ggtgttacag acatcaagaa ccaacacaag
    99361 tcaggtgacc ctgtcgacgc tcgtcgtgga ttacacaaca agtcaatcga cgaagtggcg
    99421 caagcaacta aggacgctat cacagcagat acgactttga ctgaagctga aaaagaaaca
    99481 caacgtggca atgttgataa agaagcaact aaagctaaag aagaacttgc taaggctaaa
    99541 gatgctgatg ctttagacaa agcgtacggt gacggtgtaa ccagcatcaa gaaccaacac
    99601 aagtcaggta aaggtcttga cgttcgtaaa gatgagcaca agaaagctct tgaagctgtt
    99661 gctaaacgtg tcactgctga aattgaggct gatccaacct taacaccaga agtgagagaa
    99721 caacaaaaag cagaggttca aaaagagctt gaacttgcga ctgataagat tgctgaagct
    99781 aaagatgcag atgaagcaga caaagcttac ggtgacggtg tcacagcgat cgaaaatgcc
    99841 cacgttattg gtaaaggtat cgaagctcgt aaagaccttg ctaagaaaga ccttgctgaa
    99901 gctgctgcta agacaaaagc tctcattatt gaagacaaaa cgcttactga tgatcaacgt
    99961 aaagaacagt tattaggtgt tgatacagag tatgctaaag gtatcgagaa tattgatgca
   100021 gctaaggatg ctgcaggtgt tgataaagca tatagtgacg gtgttcgtga catcctggca
   100081 cagtacaaag aaggtcaaaa ccttaatgat cgtcgtaatg ctgccaaaga atttcttctt
   100141 aaagaagcag acaaagtgac gaaactaatc aatgatgatc caaccttgac tcatgaccaa
   100201 aaagttgatc aaatcaacaa agttgaacaa gctaagttag acgcaatcaa gtctgttgat
   100261 gatgctcaaa cagctgatgc tatcaatgat gctcttggta agggtattga aaacatcaac
   100321 aaccaatacc aacatggcga tggcgttgat gttcgtaaag cgactgccaa aggcgatctt
   100381 gaaaaagaag ctgctaaagt gaaagctctt attgctaagg atccgacctt aactcaagct
   100441 gataaagaca aacaaacagc agcggttgac gcagctaaga atacagcaat tgcagcggtt
   100501 gataaagcga caacagctga aggcattaac caagaacttg gtaaaggcat cacagctatc
   100561 aataaagctt accgtccagg tgaaggtgtt aaagcacgta aagaagccgc taaggctgat
   100621 cttgaaagag aagctgctaa ggttcgtgaa gctatcgcta acgacccaac cttaacaaaa
   100681 gctgataaag ctaaacaaac agaagctgtt gctaaagctc ttaaagctgc tatcgcagcg
   100741 gttgataaag cgacaacagc tgaaggcatt aaccaagaac ttggtaaagg catcacagct
   100801 atcaacaaag cttaccgtcc aggtgaaggt gttgaagcac ataaagaagc tgctaaagct
   100861 aatcttgaaa aagtagctaa agaaactaaa gctcttattt caggagaccg ttacttgagc
   100921 gaaactgaaa aagcagtcca aaaacaagct gttgagcaag ctcttgcgaa agcacttggt
   100981 caagttgagg ctgctaagac agttgaagct gttaagttgg cagaaaacct tggtactgta
   101041 gctatccgtt cagcatatgt tgctggttta gctaaagata ctgatcaagc aacagctgct
   101101 cttaacgaag cgaaacaagc tgctattgaa gctcttaaac aagctgcggc agaaacactt
   101161 gctaagatta caactgatgc taaattgact gaagctcaaa aagctgaaca atcagaaaat
   101221 gtatcattag cgcttaagac ggctattgcg actgttcgtt cagcacaatc tattgcgtct
   101281 gtgaaagaag caaaagataa aggtattact gctatccgtg cagcctatgt gcctaataag
   101341 gcagtcgcaa aatcatcgtc agcgaaccat cttccaaaat caggtgatgc aaactcaatt
   101401 gttcttgttg gcttaggagt tatgtctctt cttttaggta tggtgcttta tagcaagaaa
   101461 aaagaaagta aagactaaga acctggcttt atccaaattg ctttctacta tattttcaaa
   101521 ccaaggtgat tttgaaatca ccttggtttt ttggatcgta agggcccaag aacggagtgt
   101581 attgaaaaca tttcaaagtt caattgaaaa ggcaacagtt ttctgtacag aatttataag
   101641 gcgttttttg ttcaaacgat gactcattta gttatgggtg tttgataatt aagattactg
   101701 tgaaggtgat ggtagttcca ccagtggact tcctccttgg ttttaagggt taattgttct
   101761 agtgaataaa aggtttcttg gtcgcgcagt agggatcaag cgagcagata aaatgcctat
   101821 tttaaatcag ttcgagcgaa ttttcgtgct acaagctcaa aagaggctac taaagtatta
   101881 gaatagttta tctctgagtg aagttcttgt tataagaaga tgatgttatc gtttgaaacg
   101941 accaaaaaat ctcctaactt ttactaattt ctacatgaaa tctagtccag tatttactct
   102001 acaaccttga ttgattcgct taataaagac atttctactt gat
//
LOCUS       DAXWIQ010000002        74303 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000002 DAXWIQ010000000
VERSION     DAXWIQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 74303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74303
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.002"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            43..1386
                     /gene="murT"
                     /locus_tag="ACGEUI_000091"
     CDS             43..1386
                     /gene="murT"
                     /locus_tag="ACGEUI_000091"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HFF8164968.1"
                     /translation="MKMKTLLGIIAGKAAQSILTKLGRGSTYPGRLALACDKDILKDL
                     SKDYDIVVVTGTNGKTLTTALTVGILKEAFGEIITNPSGANMITGITSTFLAAKKGKS
                     ERQIAVLEIDEASLPRITTYLKPSLFVYTNIFRDQMDRYGEIYTTYQMIVDGARNAPK
                     ATILANGDSPIFSSKDIVNPVQYYGFDTAKHAPQLAHYNTEGILCPKCEHILQYRLNT
                     YANLGDFVCLNCQFQRPTLDYQLTELTAITHQSSEFVIDGQNYKINVGGLYNIYNALA
                     AVSVAEFFGVSPEKIKAGFNKSKAVFGRQETFTVGDKSCTLILIKNPVGASQALEMIQ
                     LADYPFSLSVLLNANYADGIDTSWIWDANFELITQMPITEINAGGVRHSEIARRLRVT
                     GFDDTKIKQAEKLEQIIETIEKQEAKHAYILATYTAMLEFRSLLADRHVVEKEMI"
     gene            1386..2177
                     /gene="gatD"
                     /locus_tag="ACGEUI_000092"
     CDS             1386..2177
                     /gene="gatD"
                     /locus_tag="ACGEUI_000092"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit GatD"
                     /protein_id="HFF8164969.1"
                     /translation="MTYTSLKSPENQDYIYDLTIAHLYGNLMNTYGDNGNILMLKYVA
                     EKLGARVTVDIVSINDTFEQDDYDIVFFGGGQDYEQSIVAKDLPSKKAALADYIANNK
                     VVLAICGGFQLLGQYYVQANGVKIDGLGIMGHYTLNQHQNRFIGDIKIHNDEFNETYY
                     GFENHQGRTFLSGDEKPLGRVVYGNGNNKEDQTEGVHYKNVYGSYFHGPILSRNVNLA
                     YRLVTTALKKKYGSAISLPSYDDILKQEITEEYADLKSKASFNKV"
     gene            complement(2286..3275)
                     /locus_tag="ACGEUI_000093"
     CDS             complement(2286..3275)
                     /locus_tag="ACGEUI_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007893175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFF8164970.1"
                     /translation="MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIG
                     KHQNTIQEINKEYIDEHGIHVVRRLSGGGAVYHDLNNLNYTIISNKTAEGAFDFKTFS
                     QPVIATLADLGVTANFTGRNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGD
                     ALKVSKDKIESKGVKSVRARVTNILNELPEKITVEEFSDKILAKMKETYPDMTEYVLS
                     EDELAKIEQSAKEQFGSWDWTYGKAPEYTIERNVRYPAGKISTFANVENSIIKNLKIY
                     GDFFGIKDVQDIENLLIGCKYEYRDVFERLKTIDTTQYFSRMTVEEVAKAIVS"
     gene            3508..5925
                     /gene="hylA"
                     /locus_tag="ACGEUI_000094"
     CDS             3508..5925
                     /gene="hylA"
                     /locus_tag="ACGEUI_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HFF8164971.1"
                     /translation="MNTYFCTHHKQLLLYSNLFLSFAMMGQGTAIYADTLTSNSEPNN
                     TYFQTQTLTTTDSEKKVVQPQQKDYYTELLDQWNSIIAGNDAYDKTNPDMVTFHNKAE
                     KDAQNIIKSYQGPDHENRTYLWEHAKDYSASANITKTYRNIEKIAKQITNPESCYYQD
                     SKAIAIVKDGMAFMYEHAYNLDRENHQTTGKENKENWWVYEIGTPRAINNTLSLMYPY
                     FTQEEILKYTAPIEKFVPDPTRFRVRAANFSPFEANSGNLIDMGRVKLISGILRKDDL
                     EISDTIKAIEKVFTLVDEGNGFYQDGSLIDHVVTNAQSPLYKKGIAYTGAYGNVLIDG
                     LSQLIPIIQKTKSPIKADKMATIYHWINHSFFPIIVRGEMMDMTRGRSISRFNAQSHV
                     AGIEALRAILRIADMSEEPHRLALKTRIKTLVTQGNAFYNVYDNLKTYHDIKLMKELL
                     SDTSVPVQKLDSYVASFNSMDKLALYNNKHDFAFGLSMFSNRTQNYEAMNNENLHGWF
                     TSDGMFYLYNNDLGHYSENYWATVNPYRLPGTTETEQKPLEGTPENIKTNYQQVGMTG
                     LSDDAFVASKKLNNTSALAAMTFTNWNKSLTLNKGWFILGNKIIFVGSNIKNQSSHKA
                     YTTIEQRKENQKYPYCSYVNNQPVDLNNQLVDFTNTKSIFLESDDPAQNIGYYFFKPT
                     TLSISKALQTGKWQNIKADDKSPEAIKEVSNTFITIMQNHTQDGDRYAYMMLPNMTRQ
                     EFETYISKLDIDLLENNDKLAAVYDHDSQQMHVIHYGKKATMFSNHNLSHQGFYSFPH
                     PVRQNQQ"
     gene            complement(6152..6289)
                     /locus_tag="ACGEUI_000095"
                     /pseudo
     CDS             complement(6152..6289)
                     /locus_tag="ACGEUI_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611514.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6599..8362)
                     /gene="lpdA"
                     /locus_tag="ACGEUI_000096"
     CDS             complement(6599..8362)
                     /gene="lpdA"
                     /locus_tag="ACGEUI_000096"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFF8164972.1"
                     /translation="MAVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKITRQAGETVPVTEVIGYIGAEGESVEVSSPAASDVNVARTTEDL
                     EAAGLEVPKAPAQAASAAPKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEK
                     SEFGGTCLNVGCIPTKTYLKNAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVV
                     KTLTGGVQGLLKANKVTIFNGLGQVNPDKTVTIGSQTIKGRNVILATGSKVSRINIPG
                     IDSKLVLTSDDILDLREMPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAM
                     DKEVSLELQKILSKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVAEKALLSIGRVS
                     QMNGLENLNLEMDRNRIKVNDYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAM
                     HGNTTRKANLKYTPAAVYTHPEVAMVGLTEEQAREQYGDVLIGKNSFTGNGRAIASNE
                     AHGFVKVIADAKYHEILGVHIIGPAAAEMINEAATIMESELTVDELLLSIHGHPTFSE
                     VMYEAFADVLGEAIHNPPKRK"
     gene            complement(8689..10098)
                     /locus_tag="ACGEUI_000097"
     CDS             complement(8689..10098)
                     /locus_tag="ACGEUI_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HFF8164973.1"
                     /translation="MAFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKITRQAGDTVPVTEVIGYIGAEGESVDTIASSEKTTEIPVPASAD
                     AGPAVAPKENVASPAPQVAATAIPQGNGGKVRATPAARKAAAEMGIDLGQVPGTGPKG
                     RVHKEDVENFKGAQPKASPLARKIAADKGIDLATVSGTGFNGKVMKEDIMAILEAAKP
                     AEAKAPAAKEEKVVDLPEGVEHKPMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMI
                     ALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKPEHEYMNASLINDANDIELHRFVN
                     LGIAVGLDDGLIVPVIHGANKMCLSDFVLASKDVIKKAQTGKLKAAEMSGSTFSITNL
                     GMFGTKTFNPIINQPNSAILGVGATIPTPTVVDGEIVSRPIMAMCLTIDHRLVDGMNG
                     AKFMVDLKKLMENPFELLI"
     gene            complement(10283..11281)
                     /locus_tag="ACGEUI_000098"
     CDS             complement(10283..11281)
                     /locus_tag="ACGEUI_000098"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFF8164974.1"
                     /translation="MSETKLMALREAVNLAMTEEMRKDENIFLMGEDVGVYGGDFGTS
                     VGMIEEFGPKRVKDTPISEAAISGAAIGAAITGLRPIVDVTFMDFLTIMMDAIVNNGA
                     KNNYMFGGGLITPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGNANDAKGL
                     LKSAIRDNNIVLFMEPKALYGKKEEVNQDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVLQAAEEVAADGINVEVVDPRTLIPLDKELIIESVKKTGKLMLVNDAYKTGGFIGEI
                     ATMITESEAFDYLDHPIVRLASEDVPVPYARVLEQAILPDVEKIKAAIVKMANKGN"
     gene            complement(11340..12308)
                     /locus_tag="ACGEUI_000099"
     CDS             complement(11340..12308)
                     /locus_tag="ACGEUI_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HFF8164975.1"
                     /translation="MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVG
                     EEAANVGAVAHLSYDDIIFSNHRGHGQSIAKDMDLNKMMAELAGKATGVSKGRGGSMH
                     LADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNM
                     AATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGVPGFYCEDGNDVMAVYET
                     MGKAVEHVRGGNGPAIVEVESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLT
                     SEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAFEDVWVD"
     gene            complement(12593..14500)
                     /locus_tag="ACGEUI_000100"
     CDS             complement(12593..14500)
                     /locus_tag="ACGEUI_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8164976.1"
                     /translation="MIILQGNKLERSFSGDVLFQNISLQVDERDRIALVGPNGAGKST
                     LLKLLVGEETPTSGEVNTKKDLTLSYLAQNSRFESDQTIYEEMLKVFEALRQDEKRLR
                     QMEMDMATVSGQVLTRLMTDYDLLTEHFRQQGGFTYESDIKAILNGFKFDESMWQMTI
                     AELSGGQNTRLALAKMLLEKSELLVLDEPTNHLDIETIAWLENYLANYQGALIIVSHD
                     RYFLDKVATVTLDLTPNGLDRYSGNYSRFMALKAEKLVAEEKQFDKQQKEIAKLEDFV
                     QKNIVRASTTKRAQARRKQLEKIERLDKPTGGRKSAHMTFHAEKPSGNVVLRVEEAAI
                     GYGDQVLSEPINVDINKLDAIAVVGPNGIGKSTLIKSIIGQLPLLKGQLKYGANVETG
                     YYDQTQSHLTSSNTVLEELWQDFSTTPEVDIRNRLGAFLFSGDDVKKSVAMLSGGEKA
                     RLLLAKLSMENNNFLVLDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRLAT
                     KVLEITENGSTLYLGDYDYYLEKKAELEELARLAAGETVEETKEASATDYQLQKANQK
                     ERRRLTRRYEEIEARLETIEERIGAIQEDMHASNDTAQLIAWQKEWDQLDQEQEALME
                     EWETIAEQIES"
     gene            complement(14546..14872)
                     /locus_tag="ACGEUI_000101"
     CDS             complement(14546..14872)
                     /locus_tag="ACGEUI_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8164977.1"
                     /translation="MKQLLAIILWLPKLIVKMFWHLIKGFLQTILLVTIIIIGLMYYS
                     NHSDSVLANKISIVTEQVVQIFDILTQKPSAKTRHGSGNSHSTFLVSAKEGNQRLKKS
                     SISFGN"
     gene            complement(14902..15687)
                     /locus_tag="ACGEUI_000102"
     CDS             complement(14902..15687)
                     /locus_tag="ACGEUI_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8164978.1"
                     /translation="MASIAIEYHSVVLGMERKVNVIYPDQSEIPKKDQGDKDIPVLYL
                     LHGMGGNENSWQKRTAIERLLRHTNLIVVMPSTDLGWYTDTAYGLNYYRALSQELPQV
                     LAAFFPNMTQKREKTFVAGLSMGGYGAFKWALKSNRFSYAASFSGALDFSPETLLEGK
                     LGELAYWQGVFGQFDDPDLDKHYLKNMVAESDGKTKFYAWCGYEDFLFATNEKAIADF
                     QAQGLDIDYHKGHGKHEWYYWNQQLEVLLEWLPINYQKEERLS"
     gene            complement(15820..17481)
                     /locus_tag="ACGEUI_000103"
     CDS             complement(15820..17481)
                     /locus_tag="ACGEUI_000103"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFF8164979.1"
                     /translation="MTDIKMIALGGVREYGKNFYLVEINDSMFILDAGLKYPENEQLG
                     VDLVIPNLDYVIENKGKVQGIFLSHGHADAIGALPYLLAEVSAPVFGSELTIELAKLF
                     VKSNNSTKKFNNFHVVDSDTEIEFKDGLVSFFRTTHSIPESMGIVIGTDKGNIIYTGD
                     FKFDQAAREGYQTDLLRLAEIGKEGVLALLSDSVNATSNDQIASESEVGEEMDSVISD
                     ADGRVIVAAVASNLVRIQQVFDSATAHGRRVVLTGTDAENIVRTALRLEKLMITDERL
                     LIKPKDMSKFEDHELIILEAGRMGEPINSLQKMAAGRHRYVQIKEGDLVYIVTTPSTA
                     KEAMVARVENLIYKAGGSVKLITQNLRVSGHANGRDLQLLMNLLKPQYLFPVQGEYRD
                     LAAHAKLAEEVGIFPENIHILKRGDIMVLNDEGFLHEGGVPASDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVAITVSKKEKRIISKAKVNTRGFVYVKKSHDILRESAELVNTT
                     VGNYLKKDTFDWGELKGNVRDDLSKFLFEQTKRRPAILPVVMEVR"
     gene            complement(17685..18443)
                     /locus_tag="ACGEUI_000104"
     CDS             complement(17685..18443)
                     /locus_tag="ACGEUI_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8164980.1"
                     /translation="MTKIIELINATVDVDNGFEDAKTILDNVTLTIYEHDFLTILGGN
                     GAGKSTLFNVIAGTLSLTRGQIRILGQDVTHWPAEKRALYLSRVFQDSKMGTAPRMTV
                     AENLLIARQRGEKRSLASRKITEHLASFEDLVKRTGNGLEKHLETPAGLLSGGQRQAL
                     SLLMATLKKPALLLLDEHTAALDPKTSQSLMQLTDEFVTKDGLTALMITHHMEDALTY
                     GNRLIVMKDGNIIKDLNQMEKEQLTITDYYQLFD"
     gene            complement(18440..19309)
                     /locus_tag="ACGEUI_000105"
     CDS             complement(18440..19309)
                     /locus_tag="ACGEUI_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8164981.1"
                     /translation="MIISSVSQGLIWGVLGLGIYLTFRILNFPDMTTEGSFPLGGAVA
                     VTAISLGWNPFLSTLLGMLSGALAGFLTGLLYTKGKMPTLLAGILVMTSCNSIMLMVM
                     GRANLGLHDHKRIQDCLPFSIDLNSLLTGLITVVIVISVLIYFLYTNLGQAYIATGDN
                     KDMAKSFGINTDWMEVMGLVVSNSLIALSGALVSQQDGYADVSKGIGVIVIGLASIIV
                     GEVLYSTGLTLLERLIAIVIGSILYQFLISVVITLGFNTSYLKLISALVLALCLMIPV
                     VKERFFKGVRLTR"
     gene            complement(19655..20653)
                     /gene="trpX"
                     /locus_tag="ACGEUI_000106"
     CDS             complement(19655..20653)
                     /gene="trpX"
                     /locus_tag="ACGEUI_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8164982.1"
                     /translation="MKNKSLIATLLVLTVIVIGSLLSKGVSKENRDLANQQNITIGIL
                     QFVTHEALDDIKRGIEDQLKKQMPQKQNVVIKVMNAEGDQSKIQTMSRQLVQSGSDIV
                     IGIATPAAQGLAATSKDIPVVMSAVSDPVGSRLVMQLDQPEANVTGLSNKVPVKQTID
                     LMKKLTPHVKTVGILYASNEDNSLSQVKEFRRLARKKGYQVISYAVPSTNEVPATMSV
                     MLGKVDAVFIPQDNTIASAFSSVMTTSKAAKIPVYTSVDRMVEKGGLAAISQNQYDLG
                     VQTANQVLKLIKGKRVVDVPVKVVDIGQPLINKNVAAELGIAIKKEDFPSASFIEN"
     gene            21007..22659
                     /gene="fbp54"
                     /locus_tag="ACGEUI_000107"
     CDS             21007..22659
                     /gene="fbp54"
                     /locus_tag="ACGEUI_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein Fbp54"
                     /protein_id="HFF8164983.1"
                     /translation="MSFDGFFLHHLTNELKENLLYGRIQKVNQPFERELVLTIRNHRK
                     NYKLLLSAHPVFGRVQITQADFQNPQVPNTFTMIMRKYLQGAVIEQLEQIDNDRIIEI
                     KVSNKNEIGDAIQATLIIEIMGKHSNIILVDRAENKIIESIKHVGFSQNSYRTILPGS
                     TYIEPPKTAAVNPFTITDVPLFEILQTQELTVKSLQQHFQGLGRDTAKELAELLTTDK
                     LKRFREFFARPTQANLTTASFAPVLFSDSHATFETLSDMLDHFYQDKAERDRINQQAS
                     DLIHRVQTELDKNRNKLSKQEAELLATENAELFRQKGELLTTYLSLVPNNQDSVILDN
                     YYTGEKIEIALDKALTPNQNAQRYFKKYQKLKEAVKHLSGLIADTKQSITYFESVDYN
                     LSQASIDDIEDIREELYQAGFLKSRQRDKRHKRKKPEQYLASDGTTILMVGRNNLQNE
                     ELTFKMAKKGELWFHAKDIPGSHVIIKDNLDPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLHKPSGAKPGFVTYTGQKTLRVTPDQAKILSMKLS"
     gene            complement(22718..23965)
                     /locus_tag="ACGEUI_000108"
     CDS             complement(22718..23965)
                     /locus_tag="ACGEUI_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFF8164984.1"
                     /translation="MLNSEKMIASLDQQDLAHAEKYFQKALKEDDADSLIALGEYLES
                     IGFLPHAKRIYLQLADDYPELNINLAQIAAEDDAIEEAFLYLDKVSKDSPNYLSALLV
                     MADLYDMEGLTEVAREKLLQAVGISPEPLVIFGLAEIDMSLQHFKEAIDYYAQLDNRQ
                     ILELTGISTYQRIGRAYASLGKFEAAIEFLEKAVAIEYEDETVFELATLMYDQENYQK
                     ANLYFKQLETINPDYPGYEYGYALSLHEEHKTSEALRLVQQGLRKNAFDSQLLLLASQ
                     LSYELHDRQNAENYLLQAKEVAVDDEEILMRLVTLYFDAERFEEVIALNRETIDNVLT
                     KWTIAKAYHALEQEEVALALYNEISADLAENPEFLQDYAYLLREFGQFHKAIQMATAY
                     LRQVPDDVNMQDFLDHIKHQQSE"
     gene            complement(23955..25136)
                     /locus_tag="ACGEUI_000109"
     CDS             complement(23955..25136)
                     /locus_tag="ACGEUI_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8164985.1"
                     /translation="MPLIPEKSRTDSLFYKWFLNNQATMALVITLLAFLTIFVFTKIS
                     FLFMPVISFFAVIMLPLVISTILYYLTKPLVDLINHLGPNRTTSIFIVFGLITLLFVW
                     AISGFVPMVQTQLTSFIEDLPKYVGKVNEEANKLLENEWLVSYKPQLQDMLTHTSQKA
                     LDYAQSFSKNAIDWAGNFAGAIARITVAIIISPFILFYFLRDSSHMKNGLVNVLPLKL
                     RVPMVRVLGDINKQLSGYVQGQVTVAIVVGFMFSIMFSLVGLKYAITFGIIAGFLNMI
                     PYLGSFLAMIPVVIMAMVQGPFMLVKVLVIFMIEQTIEGRFVAPLVLGNKLSIHPITI
                     MFLLLTAGSMFGVWGVFLVIPIYASVKVVIKELFDWYKKVSGLYDEEVLVIEEVKDHV
                     K"
     gene            complement(25194..25670)
                     /locus_tag="ACGEUI_000110"
     CDS             complement(25194..25670)
                     /locus_tag="ACGEUI_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFF8164986.1"
                     /translation="MTQLATICYIDNGDSLLLLHRNKKENDVHKGKWISVGGKLEAGE
                     TPDECARREILEETHLTVTEMAFKGIITFPEFTPGHDWYTYVFKVTGFEGDLISDEES
                     REGTLEWVPYDQVLEKPTWEGDYDIFKWILEDRSFFSAKFTYDQNNQLMDKSVTFY"
     gene            complement(25891..26931)
                     /gene="rfbB"
                     /locus_tag="ACGEUI_000111"
     CDS             complement(25891..26931)
                     /gene="rfbB"
                     /locus_tag="ACGEUI_000111"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFF8164987.1"
                     /translation="MYKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGNRA
                     NIEAILGDRVELVVGDIADAELVDKLAAKTDVIVHYAAESHNDNSLEDPSPFIHTNFI
                     GTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGQGEGPGEKFTAETKYNPSSPYS
                     STKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLYGE
                     GKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKDAY
                     DHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKDAV
                     EAKYAKTQEVIK"
     gene            complement(27175..27768)
                     /locus_tag="ACGEUI_000112"
     CDS             complement(27175..27768)
                     /locus_tag="ACGEUI_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HFF8164988.1"
                     /translation="MTETFFDKPLACREIKEIPGLLEFDIPVRGDNRGWFKENFQKEK
                     MLPIGFPERFFEEGKLQNNVSFSRQHVLRGLHAEPWDKYISVADDGKVLGAWVDLREG
                     ETFGNVYQTVIDASKGMFVPRGVANGFQVLSETVSYSYLVNDYWALDLKPKYAFVNYA
                     DPSLGITWENLAAAEVSEADKNHPLLSDVKPLKPKDL"
     gene            complement(27768..28637)
                     /gene="rfbA"
                     /locus_tag="ACGEUI_000113"
     CDS             complement(27768..28637)
                     /gene="rfbA"
                     /locus_tag="ACGEUI_000113"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFF8164989.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDVLIISTPQDLPRFEELLGDGSEFGISLSYKEQPSPDGLAQAFIIGEEFIGDDRV
                     ALILGDNIYHGNGLTKMLQKAAAKEKGATVFGYQVKDPERFGVVEFDENMNAISIEEK
                     PEVPKSHFAVTGLYFYDNDVVEIAKNIKPSARGELEITDVNKAYLERGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNAQVANLEEIAYRMGYISKEDVHKLAQSLKKN
                     EYGQYLLRLIGEA"
     gene            complement(28695..29801)
                     /locus_tag="ACGEUI_000114"
     CDS             complement(28695..29801)
                     /locus_tag="ACGEUI_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8164990.1"
                     /translation="MKIAIIGAGIVGSTAAYYLQQSGQKEVTIFDHGQGQATKAAAGI
                     ISPWFSKRRNKVWYRMARLGADFYQQLINDLKEDGFATDFYQQNGIYVLKKQEEKLRD
                     LYELALARKVESPIIGELAVKNRKELGNDFKGLIGFDNCLYASGAARVEGAALCETLL
                     KASGYPVIRQKVTLKQQGSGYEIAGHYFDQVILAAGAWLPDLLRPLGYQVDVRPQKGQ
                     LLDYDVHHIISDTYPVVMPEGEIDLIPFNQGKISVGTSHENDKGYDLEPDWQVLEKLE
                     MQALTYLPLLKEATQKTCRVGIRAYTSDYSPFYGQVSGLKNLYTASGLGSSGLTVGPL
                     IGYELAQLLLGHEGLLTPSDYSPEPYLLRSTGKA"
     gene            complement(29841..30629)
                     /locus_tag="ACGEUI_000115"
     CDS             complement(29841..30629)
                     /locus_tag="ACGEUI_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019781659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFF8164991.1"
                     /translation="MKAKTLIDAYEAFCPLDLSMEGDVKGLQMGSLDKDIRKVMITLD
                     IRESTVAEAIKNEVDLIITKHAPIFKPLKDLVSSPQRDILLDLVKHDISVYVSHTNID
                     IVPGGLNDWFCDLLEIKEATYLSETKEGFGIGRIGTVKEQALEELASKVKRVFDLDTV
                     RLIRYDKENPLISKIAICGGSGGEFYQDAVQKGADVYITGDIYYHTAQEMLTEGLFAV
                     DPGHHIEVLFTEKLKEKLQGWKEENGWDVSIISSKASTNPFSHL"
     gene            complement(30619..31305)
                     /locus_tag="ACGEUI_000116"
     CDS             complement(30619..31305)
                     /locus_tag="ACGEUI_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFF8164992.1"
                     /translation="MDSQLSNRLAQVAAYVPKGVKLLDVGSDHAYLPIFLVETNQISA
                     AIAGEVVRGPYESALKNVTQSGLAEHIQVRLANGLAAFEEADDVTAITICGMGGRLIA
                     DILEAGKEKLQGIERLVLQPNNREDDLRAWLSVNAFKIVAETIMAENDKYYEIIVAEH
                     GEKALSATELRFGPYLSQEKSVVFKEKWQREMDKLAYALSCIPEEKTQERQLLLTKIQ
                     QIKEVIVHES"
     gene            complement(31377..32033)
                     /gene="nth"
                     /locus_tag="ACGEUI_000117"
     CDS             complement(31377..32033)
                     /gene="nth"
                     /locus_tag="ACGEUI_000117"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFF8164993.1"
                     /translation="MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQT
                     TDKAVNKVTPGLWQSYPEIEDLAFAELSDVENALRTIGLYKNKAKNIIKTAQAIRDDF
                     KGQVPKTHKELESLPGVGRKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVK
                     QIEADLMAKIPKKDWIITHHRLIFFGRYHCLAKKPKCEICPVQSYCKYYQDTYGKSKA
                     "
     gene            complement(32030..32713)
                     /locus_tag="ACGEUI_000118"
     CDS             complement(32030..32713)
                     /locus_tag="ACGEUI_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HFF8164994.1"
                     /translation="MSFLEHYKSGNLVIPSALLFHYKDLFKSSDDFLVWQFFYLQNTT
                     KRDDLAPSQIAHALGKSVADINKIISSLTNQGLLDMRTIELTGEIEIIFDASPVLAKL
                     DQLFVSQTATEIDKQETPNHFKRLVDEFERELGRFLSPFELEDLEKTLRDDKTDPDLI
                     REALKEAVFNGKTNWKYIQAILRNWRKEGIVNLRQVEERRRVREGEDLSQVTISEDFL
                     SAMNLWSDS"
     gene            complement(32794..33312)
                     /locus_tag="ACGEUI_000119"
     CDS             complement(32794..33312)
                     /locus_tag="ACGEUI_000119"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFF8164995.1"
                     /translation="MDLTNYIASIKDYPKAGITFRDISPLMADGKAYSYAIREIAQYA
                     CDKDIDMVVGPEARGFIIGCPVAVELGIGFAPVRKPGKLPRDVVSADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELEGLNGRHAIR
                     NYDYKVLMQFPG"
     gene            complement(33463..35673)
                     /gene="recJ"
                     /locus_tag="ACGEUI_000120"
     CDS             complement(33463..35673)
                     /gene="recJ"
                     /locus_tag="ACGEUI_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFF8164996.1"
                     /translation="MIKSKYSWKIKDKKPDDGFFKLAKTKGLTQTAAQLIYDRGIRTE
                     EALDEFLTADLSQLHDPYLLHDMAKAVPRIRQAIEEGERILIYGDYDADGMTSASIVK
                     ETLDMMGAEPLVYLPNRFTDGYGPNQSVYKYFIEQEAVSLIITVDNGVAGHEAIRYAQ
                     EQEVDVIVTDHHSLPEELPEAFAIIHPEHPDADYPFKHLAGCGVAFKLATALLESLPT
                     DCLDLVAIGTIADMVSLTGENRVLVKNGLAMLKHTERVGLQELMSLSPIDLEHFNEDA
                     IGFQIAPQLNALGRLDDPNPAIELLTGFDDQEAQAIALMIKKKNEERKALVQDIFDQA
                     MVMVDPQKPVQVLAQAGWHPGVLGIVAGRIMETIGQTVVVLTIDNGFAKGSARSLEAI
                     NIFEALNGKRELFTAFGGHAGAAGMTLPVDNLEALSDFLCQFVIERGLDQTAKNTLTI
                     DERLSLDDLSLDILKSLDKLAPYGMDHQKPVFYVKDIRVSQARTIGQDQSHLKFKVSQ
                     GKASFDVLAFGQGSQLQEFRQATGLELAVTLSVNHWNGNTSLQFMLVDARVDGVQLLD
                     LRTKTAKVPEGIPTIEEDPNARVILINDIPEDFKTWRNQFVHKDFDAIYFKNQMKHPY
                     YLTGFGSREQFAKLYKTIYQFPEFDLRHKLTELSHYLNIEKLLLIKLIQIFEELSFVT
                     IDDGLMTVNPQAQKREISESHIYQDLKELVKFQEIMALASPKEMYDYLVKSNDQ"
     gene            complement(35670..36434)
                     /locus_tag="ACGEUI_000121"
     CDS             complement(35670..36434)
                     /locus_tag="ACGEUI_000121"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8164997.1"
                     /translation="MAQRIIVITGASGGLAQAIVKQLPKEDSLILLGRNKERLEHCYQ
                     HIDNKECLELDITNPVAIEKMVAQIYQRYGRIDVLINNAGYGAFKGFEEFSAQEIADM
                     FQVNTLASIHFACLIGQKMAEQGQGHLINIVSMAGLIASAKSSIYSATKFALIGFSNA
                     LRLELADKGVYVTTVNPGPIATKFFDQADPSGHYLESVGKFTLQPNQVAKRLVSIIGK
                     NKRELNLPFSLAVTHQFYTLFPKLSDYLARKVFNYK"
     gene            complement(36434..37363)
                     /gene="rnz"
                     /locus_tag="ACGEUI_000122"
     CDS             complement(36434..37363)
                     /gene="rnz"
                     /locus_tag="ACGEUI_000122"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFF8164998.1"
                     /translation="MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKIRKIFITHLHGDHIFGLPGFLSSRSFQASEEQTDLDIYGPIGIKTYV
                     LTSLKVSGARVPYQIHFHEFDDKSLGKIMETDKFEVYAERLAHTIFCMGYRVVQKDLE
                     GTLDAEALKAAGVPFGPLFGKIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTR
                     KTSASVKLAKDADVLVHESTYGKGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVS
                     ARFLGRDCRQMEKDAATIFENVKMVQDLEEVII"
     gene            complement(37378..38013)
                     /locus_tag="ACGEUI_000123"
     CDS             complement(37378..38013)
                     /locus_tag="ACGEUI_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFF8164999.1"
                     /translation="MNNYIDLAKTYGGFTSLDTNYLNHLLASLTDQQKLAFITPPPSV
                     INAYFAEIYQKQSPQAATDYYFNLSKALGLFTDQPSFEEEKPFVRLNLSGKAYGFAYQ
                     NDQEVALVFSEKAEPKKPELFFELTQIFPQYMVYEDKGQLKMQAKQFEQGECEDITPD
                     DTLLSKIYRLANGITMLKGFNVEELWALSQTFSGQKYYDFAQREFMIYITQ"
     gene            complement(38006..39244)
                     /gene="hflX"
                     /locus_tag="ACGEUI_000124"
     CDS             complement(38006..39244)
                     /gene="hflX"
                     /locus_tag="ACGEUI_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFF8165000.1"
                     /translation="MIETKRQQERVILLGVELQTTEHFDMSMTELANLAKTAGVKVMA
                     SFSQKRERYDSKTFIGSGKLDEIKAIVEADEIDAVIVNNRLTARQNANLEAVLEVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQIADIERQLTQVEKNRQTIRDRRVGSDTFKIGLIGYTNAGKSTIMNLL
                     TDDSHYEANELFATLDATTKQLYLENQFQATLTDTVGFIQDLPTELVAAFKSTLEESK
                     YVDLLLHVIDASDPNHSEQEKVVLNLLKELDMLNIPRLAIYNKVDIAEQFTATAFPNI
                     RISARSKDSKILLRRLIIDQIRDQFVPFRIKVHQDKAYKLYDLNRVALLDHYTFDQEI
                     EDISGYISPKQQWRLDDFYE"
     gene            complement(39317..40216)
                     /gene="miaA"
                     /locus_tag="ACGEUI_000125"
     CDS             complement(39317..40216)
                     /gene="miaA"
                     /locus_tag="ACGEUI_000125"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFF8165001.1"
                     /translation="MTKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQL
                     DIGTAKATQEEQEAAVHHLIDIREVTESYSAYDFVQDAQKSISDIVSRGKLPIIVGGT
                     GLYLQSLLEGYHLGGQVDQEAVKAYRNELEQLDDHDLYERLQVNNITIEQVNRRRAIR
                     ALELAQFADELENAETAYEPLIIGLNDDRQVIYDRINQRVNRMIENGLLEEAKWLYEH
                     YPTVQASRGIGYKELFPYFVGEMTLAEASDQLKQNTRRFAKRQLTWFRNRMAVSFTAI
                     TAPDYPQVVHDRVRDFLGQKEKS"
     gene            40350..40526
                     /locus_tag="ACGEUI_000126"
     CDS             40350..40526
                     /locus_tag="ACGEUI_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFF8165002.1"
                     /translation="MVKKYQFVKGLATGVLATAATVAGAVFAVKKTIIDPEEEKAAFI
                     EENRKKAARRRVAR"
     gene            complement(40644..41552)
                     /locus_tag="ACGEUI_000127"
     CDS             complement(40644..41552)
                     /locus_tag="ACGEUI_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFF8165003.1"
                     /translation="MKHLKTPPLVMSGLALGTLSFGNLLATYVSIFNYLGILAALFIY
                     GILLVGMVRNLNDTKMQLRQPLIASVFPTFFMTGMLLSSLFLKVTGGCWLGFLTWWLF
                     FLGNLVLIAYYQYRFVFSFSWDNVFPSWSVLFVGIAMAALTAPASRQFLLGQVIFWVC
                     LLLTAVILPFMAKKTYGIGLGQAVMPNISTFCAPLSLLSASYLATFPRPQVGMVIFLL
                     VSSQLLYAFVVVQLPRLLNRPFNPGFSAFTFPFVISATSLKMTLSFLGWQGLGWQVLL
                     LGEVLLATALVTYVYGAYLRFLFQNK"
     gene            complement(41789..42208)
                     /locus_tag="ACGEUI_000128"
     CDS             complement(41789..42208)
                     /locus_tag="ACGEUI_000128"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HFF8165004.1"
                     /translation="MPFIAKQTLKSQLIPQDNLLAESRFNEIMDFLTGDFPLVFRPMI
                     NPHRYTISQDNQALEKVKQASYKRMDIAMTHLDSLIGESGHVYRDQQTIADAYAYAMA
                     LWSQKTPKSYENYPHLAAFMAKMVEDSAVQQVLNAAH"
     gene            complement(42519..43505)
                     /locus_tag="ACGEUI_000129"
     CDS             complement(42519..43505)
                     /locus_tag="ACGEUI_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFF8165005.1"
                     /translation="MSEKIRVLLYYKYVSIENAQEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWVHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENETKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPEFVGA"
     gene            43704..44006
                     /locus_tag="ACGEUI_000130"
     CDS             43704..44006
                     /locus_tag="ACGEUI_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165006.1"
                     /translation="MTYELCLEYGTYPLSRVDAYWGEDQNPPTFIQEDRLLCHKLETM
                     NHLFHDLFVTIESQFHYVGFNMPEKRAQIRILYQEVATILKSKYKDYPIKIETFLL"
     gene            complement(44369..44440)
                     /locus_tag="ACGEUI_000131"
     tRNA            complement(44369..44440)
                     /locus_tag="ACGEUI_000131"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44405..44407),aa:Arg,seq:ccg)
     gene            complement(44553..45758)
                     /gene="rpsA"
                     /locus_tag="ACGEUI_000132"
     CDS             complement(44553..45758)
                     /gene="rpsA"
                     /locus_tag="ACGEUI_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFF8165007.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIEGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVIEEAAKEARAEVFSKISEGAVVTGTVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVSVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AQGDVVEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSVGQEVTVKVL
                     EVNAADERVSLSIKALEERPAQAEGDNKEEKRQSRPRRPKRESRRDYELPETQTGFSM
                     ADLFGDIEL"
     gene            complement(45885..45956)
                     /locus_tag="ACGEUI_000133"
     tRNA            complement(45885..45956)
                     /locus_tag="ACGEUI_000133"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45922..45924),aa:Gln,seq:ttg)
     gene            complement(46008..46088)
                     /locus_tag="ACGEUI_000134"
     tRNA            complement(46008..46088)
                     /locus_tag="ACGEUI_000134"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46052..46054),aa:Tyr,seq:gta)
     gene            complement(46149..46379)
                     /locus_tag="ACGEUI_000135"
     CDS             complement(46149..46379)
                     /locus_tag="ACGEUI_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFF8165008.1"
                     /translation="MSKKDKKIEIQLIDHKVMVNETKIDGYQLQIGKRVVGEIAELDE
                     KFAVLKNDGVDCFFKTLEQAVENIIENYNLNH"
     gene            complement(46443..47510)
                     /locus_tag="ACGEUI_000136"
     CDS             complement(46443..47510)
                     /locus_tag="ACGEUI_000136"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFF8165009.1"
                     /translation="MKKSYNRKKQKRGTMMTIAIDWDNLGFEYHKLPFRYISYYKNGQ
                     WDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNAVRLQATAD
                     RLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYI
                     FTIFAMPVGNYFKGGLAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAG
                     FSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPLSPSILPSITKYSLLQLAEERLG
                     MTVIEGDVPINELDKFVEAGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYD
                     ELVGIQFGDIEAPEGWIVKVD"
     gene            complement(47588..50047)
                     /gene="parC"
                     /locus_tag="ACGEUI_000137"
     CDS             complement(47588..50047)
                     /gene="parC"
                     /locus_tag="ACGEUI_000137"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFF8165010.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQDWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVSFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSSGISAGYATDIPPHNLSEVIDAVVYMIDHPKASLEKLMEFLPGPDFPTGGI
                     IQGADEIKKAYETGKGRVVVRSRTEIEELKGGKQQIIVTEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIVEVRDESDRTGLRIAIELKKEADSQTILNYLLKYTDLQVNYNFNMVAIDH
                     FTPRQVGLQKILSSYISHRKDIIIERSKFDKAKAEKRLHIVEGLIRVLSILDEIIALI
                     RSSDNKADAKENLKVSYDFSEEQAEAIVTLQLYRLTNTDIVTLQNEENDLRDLITTLS
                     AIIGDEATMYNVMKRELREVKKKFANPRLSELQAESQIIEIDTASLIAEEETFVSVTR
                     GGYLKRTSPRSFNASSLEEVGKRDDDELIFVKQAKTTEHLLLFTTLGNVIYRPIHELT
                     DLRWKDIGEHLSQTISNFATEEEILYADIVTSFDQGLYVAVTQNGFIKRFDRKELSPW
                     RTYKSKSTKYVKLKDDKDRVVTLSPVIMEDLLLVTKNGYALRFSSQEVPIQGLKSAGV
                     KGINLKNDDSLASAFAVTSNSFFVLTQRGSLKRMAVDDIPQTSRANRGLLVLRELKTK
                     PHRVFLAGGVQSDTSAEQFDLFTDIPEEETNQQMLEVISKTGQTYEIALETLSLSERT
                     SNGSFISDTISDQEVLVARTR"
     gene            complement(50138..52087)
                     /gene="parE"
                     /locus_tag="ACGEUI_000138"
     CDS             complement(50138..52087)
                     /gene="parE"
                     /locus_tag="ACGEUI_000138"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFF8165011.1"
                     /translation="MTKKEITINNYNDDAIQVLEGLDAVRKRPGMYIGSTDATGLHHL
                     IWEIVDNAVDEALSGFGDDIKVVINKDGSVSVADSGRGMPTGQHAMGIPTVQVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSAWLEVEITRDGSVYRQRFENGGKPVTTLK
                     KVGTAPKSKSGTVVTFMPDDKIFSTIDFKFNTISERLKESAFLLKNVKMSLTDLRGDD
                     PIIEEFHYENGVQDFVEYLNEDKETLTPVIYMEGQDQDFQVEVALQYNDGFSDNILSF
                     VNNVRTKDGGSHETGLKSAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILV
                     PEQHLQFEGQTKDKLGSPLARPIVESIVSEKLTFFLLENGEVASHLVRKAIKARDARE
                     AARKARDDSRNGKKNKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTEKAKMADILKNEEINTMVYTIGAGVGADFNLEDINYDKIIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKTEKIAYAWTDGEL
                     EDLRREFGKGAILQRYKGLGEMNANQLWETTMDPETRTLIRVTIDDLARAERRVSVLM
                     GDKAAPRRQWIEDNVKFTLEENTVF"
     gene            52222..52863
                     /gene="plsY"
                     /locus_tag="ACGEUI_000139"
     CDS             52222..52863
                     /gene="plsY"
                     /locus_tag="ACGEUI_000139"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFF8165012.1"
                     /translation="MKLLLFITIAYLLGSIPTGLWIGQYFYHINLREHGSGNTGTTNT
                     FRILGVKAGTATLAIDMFKGTLSILLPIIFGMTSISSIAIGFFAVLGHTFPIFANFKG
                     GKAVATSAGVLLGFAPLYLFFLASIFVLVLYLFSMISLASVVSAIVGVLSVLTFPAIH
                     FLLPNYDYFLTFIVILLAFIIIIRHKDNISRIKHHTENLIPWGLNLSKQVPKK"
     gene            complement(52921..54189)
                     /locus_tag="ACGEUI_000140"
     CDS             complement(52921..54189)
                     /locus_tag="ACGEUI_000140"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFF8165013.1"
                     /translation="MILIKNGRVMDPKSQRDQVADVLIDGKQIVKIASAIECQEAQVI
                     DASGLIVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGVTTVVMMANTNPVISDVE
                     TLQEVLASAAKEKIHIYTNASVTQAFNGKDVTDFKALLEAGAVSFSDDGIPLESSKVL
                     KEAFDLANANQTFISLHEEDPQLNGVLGFNEGIAEEHFHFCGATGVAEYSMIARDVMI
                     AYDRQAHVHIQHLSKAESVQVVAFAQQLGAKVTAEVSPQHFSTTEDLLLIAGTSAKMN
                     PPLRTQRDRLAVIEGLKSGVITVIATDHAPHHKDEKAVDDMTKAPSGMTGLETSLSLG
                     LTHLVEPGHLTLMSLLEKMTLNPALLYGFDAGYLAENGPADLVIFADKQERLITENFA
                     SKASNSPFIGNKLKGVVKYTIADGEVVYPN"
     gene            complement(54321..54974)
                     /locus_tag="ACGEUI_000141"
     CDS             complement(54321..54974)
                     /locus_tag="ACGEUI_000141"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFF8165014.1"
                     /translation="MAHSIWHEKIKSFLPEHYYGRINHFLDEAYASGLVYPPRENVFK
                     ALQVTPLEETKVLILGQDPYHGPKQAQGLSFSVPEEISAPPSLINILKELADDIGPRD
                     HHDLSTWASQGVLLLNACLTVPAGQANGHAGLIWEPFTDAVIKVLNEKDSPVVFILWG
                     AYARKKKAFITNPKHHIIESPHPSPLSSYRGFFGSKPFSRTNAILEKEGMTGVDWLK"
     gene            complement(55107..55757)
                     /locus_tag="ACGEUI_000142"
     CDS             complement(55107..55757)
                     /locus_tag="ACGEUI_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8165015.1"
                     /translation="MINIPLMKDSLGFVLSGLPYTLGISLLSFFTGLFLGLGLALLGR
                     SRQPLIHYLVRAYISIMRGVPMIVVLFVLYFGLPYYGLELPALLCAYLGFSMVSAAYI
                     SEVFRSSIEAIDKGQWEAAKALGLPYALMVKKIILPQAFRIAVPPLGNVIIDMVKSSS
                     LAAMITVPDIFQNAKIIGGREWDYMSMYILVAFIYWLIAFLLERYQEFLENKLALV"
     gene            complement(55786..56649)
                     /locus_tag="ACGEUI_000143"
     CDS             complement(55786..56649)
                     /locus_tag="ACGEUI_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8165016.1"
                     /translation="MKKSVTLLSIGLASLLLAACAPHQSQKSSWDTIKEKGVLKVATP
                     GTYQPTSFYNDNNELVGYEVDMVKEIGKRLNIKVKFVETGFDQAFTSVDSGRVDISLN
                     NFDITPKRQKKYNISTPYKYGVGGMIVRADGSSNMAKKDLSDWKGKKAAGASGTEYMK
                     VAQKQGAELVTYDNVTGDVYLNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMS
                     DVQYNPTEQGIVMNKKDDSLKKKIDAVIKDMIKDGSLKKISETYHAGQDLTKPFGKDK
                     KIPVIDTKDVN"
     gene            complement(56767..58221)
                     /locus_tag="ACGEUI_000144"
     CDS             complement(56767..58221)
                     /locus_tag="ACGEUI_000144"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFF8165017.1"
                     /translation="MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSE
                     RFEAALEEAKQRDFSGLPFAGVPLFLKDLGQELKGHSSTSGSRLFKEYQATKTDLFVK
                     RLEALGFIILGRSNTPEFGFKNISDSSLHGPVNLPRDNTRNAGGSSGGAAALVSSGIS
                     ALATASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTR
                     NLLYYLQMEQMESPFPLATLTKDSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALRQA
                     VTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIEDTFGRPMTKDDMET
                     MTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVP
                     DSKLMANLAQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEG
                     LSMGIQLIAAKGREDLLLGIAEQFEAAGLLKIPQ"
     gene            complement(58418..59047)
                     /gene="pyrE"
                     /locus_tag="ACGEUI_000145"
     CDS             complement(58418..59047)
                     /gene="pyrE"
                     /locus_tag="ACGEUI_000145"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFF8165018.1"
                     /translation="MTLASQIATQLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLS
                     YPKTRDLIENGFVETIKAHFPEVEVIAGTATAGIPHGAIIADKMTLPFAYIRSKPKDH
                     GAGNQIEGRVLKGQKMVIIEDLISTGGSVLDAAAAASREGADVLGVVAIFTYELPKAS
                     QNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKEDQVNWQQ"
     gene            complement(59105..59797)
                     /gene="pyrF"
                     /locus_tag="ACGEUI_000146"
     CDS             complement(59105..59797)
                     /gene="pyrF"
                     /locus_tag="ACGEUI_000146"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFF8165019.1"
                     /translation="MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYVQG
                     PDIVRYIKSLGHNVFLDLKLHDIPNTVRAAMAVLKELDIDMATVHAAGGVEMLKAARE
                     GLGQGPTLIAVTQLTSTSEDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGAVCSAQEV
                     EAIKAVTPTGFTCLTPGIRPKGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPV
                     AAYQAIKAEWAG"
     gene            complement(60074..60610)
                     /locus_tag="ACGEUI_000147"
     CDS             complement(60074..60610)
                     /locus_tag="ACGEUI_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165020.1"
                     /translation="MNVTEQNSHQILIQKLLVSIHYLTLFRDELKLVERTPSILGGEF
                     PAHLVQSELGDIVAAIDTLDMQQRLIESTFWYEESAFKLMNKTLDIVDNWIKGVDHLI
                     DLCQSKEVFQIIIGDKRIRVFGVLSDVFSSLKVSALSLKEAPIPDVLYEHIQRVNLEE
                     EAFIKYYQSPKVPTGEFE"
     gene            60832..61737
                     /locus_tag="ACGEUI_000148"
     CDS             60832..61737
                     /locus_tag="ACGEUI_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8165021.1"
                     /translation="MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMG
                     ITIFNRNPKGITLTKDGVEFLSYARQIIEQTSLLEDRYKNHNTGRELFSVSSQHYAFV
                     VNAFVSLLKRTDMTRYELFLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDD
                     NHLTASPLFKAQPHIFVSKSNPLATKSLLSMDDLRDFPYLSYDQGIHNSFYFSEEMMS
                     QMPHNKSIVVSDRATLFNLMIGLDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFI
                     KHEKANLSKMGERFIEYLLEEVTFD"
     gene            complement(61816..62604)
                     /locus_tag="ACGEUI_000149"
     CDS             complement(61816..62604)
                     /locus_tag="ACGEUI_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HFF8165022.1"
                     /translation="MTNNQTLDILLDVYAYNHAFRIAKALPNIPKTALYLLEMLKERR
                     ELNLAFLAEHAAENRTIEDQYHCSLWLNQSLEDEQIANYILDLEVKVKNGAIIDFVRS
                     VSPILYRLFLRLITSEIPNFKAYIFDTKNDQYDTWHFQAMLESDHEVFKAYLSQKQSR
                     NVTTKSLADMLTLTSLPQEIKDLVFLLRHFEKAVRNPLAHLIKPFDEEELHRTTHFSS
                     QAFLENIITLATFSGVIYRREPFYFDDMNAIIKKELSLWRQSIV"
     gene            complement(62597..63310)
                     /gene="deoD"
                     /locus_tag="ACGEUI_000150"
     CDS             complement(62597..63310)
                     /gene="deoD"
                     /locus_tag="ACGEUI_000150"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFF8165023.1"
                     /translation="MSIHISAKKGDIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAIDPEVHVRE
                     LVLAQAAATNSNIIRNDFPEFDFPQIADFGLLDKAYHIAREMGVTTHVGNVLSSDVFY
                     TNMPERNMALGKLGVKAIEMEAAALYYLAAQHHVKALGIMTISDNLNDPTEDTTAEER
                     QTTFTDMMKVGLETLIAND"
     gene            complement(63973..64782)
                     /locus_tag="ACGEUI_000151"
     CDS             complement(63973..64782)
                     /locus_tag="ACGEUI_000151"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFF8165024.1"
                     /translation="MSLMTKINETKDFLVTKGIETPEFGLILGSGLGELAEEVENAIV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKA
                     LGCEGVLVTNAAGGIGYGPGTLMAITDHINMTGNNPLIGENLDEFGPRFPDMSDAYTK
                     VYRNKAHEVAEKMNIKLEDGVYMGLTGPTYETPAEIRAFKVLGADAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQHIKEDFKGLVKAILAEL"
     gene            complement(64766..65206)
                     /gene="arsC"
                     /locus_tag="ACGEUI_000152"
     CDS             complement(64766..65206)
                     /gene="arsC"
                     /locus_tag="ACGEUI_000152"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922177.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFF8165025.1"
                     /translation="MEEITIYHNPNCGTSRNVLAMIRHAGIEPTIIEYLQTPPNRETL
                     IELLQSMGISARELLRTNVPEFEAYGLANQAVAEKDIINAMLADPILINRPIVVTRKG
                     VKLCRPSETLLDILPVPLPSPYIKEDGESVNPIEVKGEKDVIDD"
     gene            complement(65225..66436)
                     /locus_tag="ACGEUI_000153"
     CDS             complement(65225..66436)
                     /locus_tag="ACGEUI_000153"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFF8165026.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDADKFFNAGVADTDSDTLGHISE
                     AAGLSVPNMAKIGLGNISRPIPLKTVPTEDNPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGPRQMETGELIV
                     YTSADPVLQIAAHEDIIPVEELYKICEYARSITLERPALLGRIIARPYVGDPGNFTRT
                     ANRHDYAVSPFQDTVLNKLADAGVPTYAVGKINDIFNGSGITNDMGHNKSNSHGIDTL
                     IKTLQLPEFTKGFSFTNLVDFDANFGHRRDPEGYRDCLHEFDNRLPEIIANMKEDDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSVSFTGNGLIPQGHFADISATVAENFGVDTA
                     MIGESFLSHLK"
     gene            complement(66513..67196)
                     /gene="rpiA"
                     /locus_tag="ACGEUI_000154"
     CDS             complement(66513..67196)
                     /gene="rpiA"
                     /locus_tag="ACGEUI_000154"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFF8165027.1"
                     /translation="MEALKKIAGVTAAQYVTDGMTIGLGTGSTAYYFVEEIGRRVKQE
                     GLQVVGVTTSSVTSKQAEVLGIPLKSIDDIDSIDLTVDGADEVDKNFNGIKGGGAALL
                     MEKIVATPTKEYIWVVDASKMVEHLGAFKLPVEVVQYGADRLFRVFEKAGYKPSFRMK
                     GDSRLVTDMQNYIIDLDLGCIKDPVAFGHLLDGTVGVVEHGLFNGMVDKVIVASKDGV
                     TVLEAPTAG"
     gene            67575..69674
                     /locus_tag="ACGEUI_000155"
     CDS             67575..69674
                     /locus_tag="ACGEUI_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8165028.1"
                     /translation="MTHFSGKDPFVNMDDIFNQLMANMGGYRSENPRYLVNGREITPE
                     EFQHYRQTGQLPVATTKATNSQMLTPKADSVLTQLGTNLTQEARQGHLDPVIGRNKEI
                     QDTAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIVNGDVPAAIKNKEIVSIDISSL
                     EAGTQYRGSFEETIQNLIQEVKEAGNIILFFDEIHQIVGAGATSSDSGSKGLADILKP
                     ALSRGELTLIGATTQDEYRNTILKNAALARRFNEVKVNAPSAEDTFHILMGIRNLYEQ
                     HHHITLPDNVLKAAVDYSIQYIPQRSLPDKAIDLLDMTAAHLAAQHPVTDLKTLETEI
                     AKQKESQEKAVAKEDFEKALAAKTRIETLQKQIEQHNQSQNVTATVNDIAESVERLTG
                     IPVSNMGTNDLERLKGISSRLKSHVIGQDEAVAAVARAIRRNRAGFDDGKRPIGSFLF
                     VGPTGVGKTELAKQLALDLFGSKDAIIRLDMSEYNDRTAVSKLIGTTAGYVGYDDNNN
                     TLTERVRRNPYAIVLLDEIEKADPQIITLLLQVLDDGRLTDGQGNTINFKNTVIIATS
                     NAGFGQQDTETSESNIMDRIAPYFRPEFLNRFNSIIKFNHLQKESLEEIVDLMLAEVN
                     QTTAKKGISLTITDDAKAHLIDLGYNHAMGARPLRRIIEQEIRDRITDYYLDHPEVKK
                     LQAILKEGQLVIRQNDQ"
     gene            complement(69732..70475)
                     /locus_tag="ACGEUI_000156"
     CDS             complement(69732..70475)
                     /locus_tag="ACGEUI_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165029.1"
                     /translation="MTQVAQGRSKGKSLLCHQCNALFVFIIFLILLAVLFGVRYRNSS
                     IEGIWRTTSIDQKLGDDFAKRLTGLHQSPLIDDSLLTSSQMILTVKNNNVDLSFSVQV
                     ERDIFVKRLAAYHQNELLKTLKENHLVVGDLSSKERQIIENSMPASHELEMILDQAFE
                     KLASQIGGKYNQKTGHLSAVVLKGKVNRILHTIDIKEEVAAGHTSFSKGLLTPNGYFD
                     YTRFGKKLELLGDEKIIFKKALKKSPSSV"
     gene            complement(70623..71222)
                     /gene="yihA"
                     /locus_tag="ACGEUI_000157"
     CDS             complement(70623..71222)
                     /gene="yihA"
                     /locus_tag="ACGEUI_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFF8165030.1"
                     /translation="MAEEQVLNTHNASILLSAANKSHYPQDDLPEIALAGRSNVGKSS
                     FINTILGRKNLARTSSKPGKTQLLNFFNIDDKLRFVDVPGYGYAKVSKSERAKWGKMI
                     EEYLTSRDNLRAVVSLVDLRHAPSKEDIQMYDFLKYYDIPVIVVATKADKIPRGKWNK
                     HESVVKKALNFDKSDTFIVFSSVERIGIDDSWDAILEQV"
     gene            complement(71232..72461)
                     /gene="clpX"
                     /locus_tag="ACGEUI_000158"
     CDS             complement(71232..72461)
                     /gene="clpX"
                     /locus_tag="ACGEUI_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFF8165031.1"
                     /translation="MAGSRTNDIKVYCSFCGKSQDDVKKIIAGNNVFICNECVALSQE
                     IIKEELAEEVLADLTEVPKPKELLDVLNQYVVGQDRAKRALSVAVYNHYKRVSFTESR
                     DDDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTV
                     ASVPPQGGRKHPNQEMIQIDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNSRKID
                     DNASYMQEIISEDIQKFGLIPEFIGRLPVVAALEQLNTSDLIQILTEPRNALVKQYQA
                     LLSYDGVELAFDKEALEAIANKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVR
                     ITKAAVEGKSKPVLETA"
     gene            complement(72591..72761)
                     /locus_tag="ACGEUI_000159"
     CDS             complement(72591..72761)
                     /locus_tag="ACGEUI_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165032.1"
                     /translation="MQRSIFGVFTAFLCVICVLCAIPAFKKNRYGLASLFLLNAFTNL
                     VNSIHAFYGTLF"
     gene            complement(72781..73278)
                     /locus_tag="ACGEUI_000160"
     CDS             complement(72781..73278)
                     /locus_tag="ACGEUI_000160"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFF8165033.1"
                     /translation="MTKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAIL
                     MGRVTFEGMNCKRLPQRQTLVMTRNRDYQVDEVLTMTSIEKVLEWYHAQDKTLYIIGG
                     NKVLEAFNGYFDRVIKTVIHHRFKGDTYRPNLDFSHFTQESQTFYARDAKNPYDFTVT
                     ALKHK"
     gene            complement(73358..74197)
                     /locus_tag="ACGEUI_000161"
     CDS             complement(73358..74197)
                     /locus_tag="ACGEUI_000161"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFF8165034.1"
                     /translation="MTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAF
                     AEYDLAKGEFPITTLRPIPIKSAIKELFWIYQDQSNSLDVLEAKYNVHYWNEWEVDQT
                     RTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMF
                     DVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNL
                     HIYDNQFDQAQELLKRQPVASQPKLVLNVPDRTNFFDIKPDDFELQNYDPVKPQLHFD
                     LAI"
BASE COUNT        22457 a        15697 c        13116 g        23033 t
ORIGIN      
        1 tttccctatc taaaatcaat aaacttatga taaaatataa gcatgaaaat gaagacatta
       61 ctcggcatta ttgctggcaa agctgctcaa tccattttaa caaaactagg gcgaggatct
      121 acttatcctg gtcgattggc tctagcttgt gataaagata ttttaaagga tttgtctaaa
      181 gactatgata ttgtggtcgt cactggtact aatggtaaaa cgttaacaac tgctcttact
      241 gtcggtattt tgaaagaggc atttggcgag ataatcacaa atccaagtgg tgccaatatg
      301 attactggaa ttacttctac ttttttagct gctaaaaaag ggaaatctga aaggcaaatt
      361 gctgttttag aaattgacga ggctagtctt cctagaatta ccacttactt aaaacctagt
      421 ctttttgttt atactaatat ttttagggat cagatggacc gctacggaga gatttacact
      481 acttatcaga tgattgtaga tggtgctcga aatgctccaa aagcaactat tctagccaat
      541 ggagatagtc ctattttctc ttctaaggac atagttaatc cagttcagta ttatggtttt
      601 gatactgcta agcatgctcc gcagctagct cattacaaca cagaagggat tttatgtcca
      661 aaatgcgaac atattcttca gtatcgtctc aatacttatg cgaatctagg agactttgtt
      721 tgcttaaact gtcagttcca aagaccaacg ttagattatc aattaactga gctcaccgct
      781 attactcacc aaagttctga atttgttatt gatggacaaa actacaagat aaatgttggt
      841 ggtctttata atatttacaa tgcgctcgct gccgtctctg ttgctgaatt tttcggtgtt
      901 tctcccgaga aaatcaaggc gggctttaac aaaagtaagg cggtttttgg aagacaagaa
      961 acttttactg ttggtgataa atcatgcacc cttatcctta tcaagaatcc tgttggagcc
     1021 agtcaggcac tggaaatgat tcaattggca gattatccat ttagcttatc agttttatta
     1081 aatgccaatt acgctgatgg cattgatacc agctggattt gggatgctaa ttttgaactc
     1141 attacccaaa tgcccattac agaaattaat gctggcggcg tccgccactc tgaaattgct
     1201 agaagactta gagtcacagg atttgacgat actaagataa aacaagctga aaagctcgag
     1261 caaatcatag aaaccattga aaaacaagaa gctaagcatg cctatatttt agcaacctat
     1321 acggcaatgt tagaattccg tagcttactt gctgaccgcc atgtcgttga aaaggagatg
     1381 atctaatgac atacacttca ctaaaatctc ccgaaaatca agattacatc tacgacctca
     1441 ctatcgccca tctctatggt aatcttatga atacttatgg tgataacggc aatattttaa
     1501 tgctcaagta tgtcgctgaa aaacttggag ctagggtcac tgttgatatt gtgtctatca
     1561 atgatacctt tgaacaagac gattacgata ttgttttctt tggtggtggt caagattatg
     1621 aacaaagcat tgttgccaag gatttgccta gcaaaaaagc tgccttagcg gattacattg
     1681 ctaataataa agtggtgctt gccatctgtg gtggcttcca attactaggg caatactatg
     1741 tccaagctaa tggggttaaa attgatggac ttggaattat gggacactac acccttaatc
     1801 agcaccaaaa tcgctttatt ggagatatta aaattcataa tgacgaattt aacgaaacct
     1861 attatggctt tgaaaatcat caaggacgta ccttcttatc tggtgacgaa aagccacttg
     1921 gacgagtggt ttatggcaac ggtaataata aagaagacca aactgaaggt gttcactata
     1981 aaaacgtcta tggtagttat ttccacggtc ctatcttatc acgcaatgtt aatcttgctt
     2041 accgcttagt aacaacagct cttaaaaaaa agtatggatc agctatttca ctacctagct
     2101 atgatgatat actgaaacaa gaaatcactg aagaatatgc cgaccttaaa agcaaagctt
     2161 cctttaataa agtttaagtt cttctttcat gtcatcaaac aaaaactcct aagtaggaca
     2221 cgatagttcg cttaggagtt ttatagtttt ttcttctgac agagcttctt aagtgattaa
     2281 aaccactaag aaacaatagc tttagcaact tcttcaacgg tcatgcgtga gaagtactgg
     2341 gtcgtatcaa tcgtttttaa acgttcaaaa acatcacggt actcatattt acaaccaatt
     2401 aataagttct caatgtcttg cacatctttg attccaaaga aatcaccata gattttaagg
     2461 tttttaatga tagaattttc aacatttgca aaggttgaaa ttttacctgc agggtaacga
     2521 acattgcgtt caatcgtata ttctggcgct ttgccgtagg tccaatccca gctaccaaat
     2581 tgctctttgg cagattgttc aatcttagcc aactcatcct ctgacaagac atattctgtc
     2641 atgtctgggt acgtttcttt catcttggcc aagattttat ctgaaaattc ctcaacggta
     2701 attttttctg gtaattcatt taaaatattg gtcacgcgag cacgaacaga cttcactcct
     2761 ttagactcaa tcttgtcctt acttacctta agagcatcgc caagaacggt catatccaca
     2821 tcaaacaaaa ggcaaccatg gtgcatcatg cgacctttgt agtaagcttg agcattaccg
     2881 cagatttttt taccatcaat ttcaatatca ttacggcctg taaagttggc tgttacaccc
     2941 aaatcagcta aagtcgcaat cacaggttgt gagaaggttt tgaaatcaaa tgcgccttcc
     3001 gctgttttat ttgaaatgat agtgtagttc aagttattca aatcatgata aaccgcacca
     3061 ccaccagata aacgtcgcac aacatgaata ccatgttcat caatgtattc tttattgatt
     3121 tcttgaatgg tattttgatg tttgccaata ataatagctg gctcattgat ccaaaggata
     3181 aacaattcgt cttcttccac tagttctcta aaagcatagg cttccaaagc gatgttaaag
     3241 gcaggattgt gacttttgtt aacgatgtat ttcataggag aaaaaatcct ttcaaaaatg
     3301 taatctacct ctctagtata atgaaaacaa atataaaaga caagtgtcct attctgtatg
     3361 acatcttcag tacctacact tattagtgca caaaaactca gccattattt atgtaagggt
     3421 ttataaaaat actttaacgt ggtatatttt tacttagtaa atcaatttac ttttattatc
     3481 tcatttttat attggaggaa tttatcagtg aatacttatt tttgcacaca ccataaacaa
     3541 ttactacttt attcaaacct attccttagc tttgctatga tgggccaagg aactgccatt
     3601 tatgccgata cactgacttc aaattcagaa cctaataata cttactttca aacgcaaacg
     3661 ctcactacta cagatagcga aaaaaaggta gtacagccac aacaaaaaga ctactatact
     3721 gaattgttag accaatggaa cagtattatc gcaggcaacg atgcttatga taaaaccaat
     3781 cctgacatgg tcacttttca taataaagct gaaaaggatg ctcaaaacat tattaaaagc
     3841 tatcaagggc ctgaccacga aaatagaact tacctttggg aacatgcaaa ggattattcc
     3901 gcttctgcta atatcacgaa aacttaccgc aatattgaaa aaatagcaaa acagatcact
     3961 aatcctgaat catgctatta tcaagatagt aaagctattg ctattgtaaa agacggtatg
     4021 gccttcatgt atgaacacgc ttataatcta gatcgtgaaa atcatcaaac aactggaaaa
     4081 gaaaacaaag aaaattggtg ggtttatgaa attggaactc ctcgtgctat taataatacc
     4141 ttatccttga tgtatcctta ttttactcaa gaagaaattc ttaaatacac agctccaatc
     4201 gaaaaatttg tgcctgaccc tactcgtttt agggttcgcg ctgccaattt ttcacctttt
     4261 gaagccaata gcggaaattt aattgatatg gggcgtgtta aactcatttc cggtattctt
     4321 cgtaaagatg atctcgaaat tagtgataca atcaaagcaa ttgagaaagt tttcacgcta
     4381 gttgatgaag gaaatggttt ttaccaagac ggttctttaa ttgatcacgt ggttactaac
     4441 gctcaaagtc cactttataa aaaaggcatt gcttacactg gagcttacgg taatgtgctt
     4501 atagatggct tatcgcaatt aattcctatt attcaaaaaa caaagtctcc tataaaagcg
     4561 gataaaatgg ctactatcta tcattggatt aaccattctt ttttccctat catcgttcgt
     4621 ggagaaatga tggatatgac tcgagggcgt tctatcagtc gttttaatgc ccaatctcat
     4681 gttgctggca ttgaagcact tcgtgctatt ttacgtattg ctgacatgtc tgaagagcct
     4741 caccgtttgg cacttaaaac acgtataaaa acactcgtca cacaagggaa tgctttttac
     4801 aatgtctatg ataatttgaa aacctatcac gatatcaaac ttatgaaaga actactaagt
     4861 gatacttctg ttccagtcca aaaacttgat agttacgtag ctagtttcaa tagtatggat
     4921 aaattggcac tatataataa taaacacgat tttgcttttg gcctatcaat gttttcgaat
     4981 cgaactcaaa attatgaagc tatgaataat gaaaatcttc atggctggtt tacttctgat
     5041 ggaatgtttt acctatacaa taacgattta ggacactaca gtgaaaacta ttgggcaacg
     5101 gtaaatccct accgcttacc tggaaccaca gaaactgagc aaaaaccact agagggaact
     5161 cctgagaata ttaaaacgaa ctatcaacaa gttggcatga ctggtctctc tgatgacgct
     5221 tttgttgcaa gtaaaaaact taataacaca agtgctctag ctgctatgac cttcactaat
     5281 tggaataaaa gcctcaccct caataaaggg tggtttatct taggaaacaa aataatcttt
     5341 gttggtagca atatcaaaaa ccaatcatct cacaaggcgt atacaactat tgaacaacga
     5401 aaagaaaatc aaaagtaccc ttactgttct tatgttaaca atcaacccgt tgacttgaat
     5461 aatcagctag ttgattttac aaacactaaa agtattttcc ttgaaagtga tgatcccgct
     5521 caaaatattg gttactactt cttcaagcca acaacactta gcataagtaa ggcacttcaa
     5581 acagggaaat ggcaaaacat aaaagctgat gacaaatcac cagaagccat caaagaagtt
     5641 tcaaatacct ttatcactat catgcaaaac catactcaag atggcgatcg ttatgcctat
     5701 atgatgcttc caaatatgac tcgtcaagaa tttgaaacct atattagcaa gcttgatatc
     5761 gacttattag aaaacaatga caaactggcc gctgtctacg atcatgatag tcaacagatg
     5821 cacgtcattc actatggaaa aaaagcaacg atgttttcaa atcataatct ttctcatcaa
     5881 ggcttttata gttttcctca tcctgtcagg caaaatcaac aataaaagct tgctgatcaa
     5941 ggaattgcag ctaaaaacaa tgctcttaac tctcataaaa ttccccacaa aagacaacga
     6001 cgcttaccgc atactgggta tcagtcctcc tctttagaat tcttaggtgg agctttagtt
     6061 gcttctttta accatatcac aaaacccttt cgtaagaaag acttaaggat ttagctgtta
     6121 ttggaaagta agggttaaca atgaaacgtt attaaagaca aaaaactaga aactgcactg
     6181 agccccaaaa gttggctact ctatttgaag taaaggattt agttctgtat tgtagaggac
     6241 taagtccttt tcgttttatt ttgatacgtt tattgttgca ataatcgatg ataatagtag
     6301 gtagaacgag tcaatttagc cgtccttaaa agaaaatcta atcagaactc gtctttatcc
     6361 attctttata atggtttttt gtcttcttct tttcagagat gcttctttca aacggagttc
     6421 tctcggtttt tttagcgcta aatgtctagc ttatcttcat agcttaatgt catacaaaaa
     6481 caccccaaac gttagggttt tatatctaac gtttggggtg cagttcatag ttgtgctagt
     6541 cttttttgtg atctgttatg tgatgatatt tttcattagt taataagaca aagacgtttt
     6601 acttacgttt tggtgggtta tggatagctt cgccaagcac atctgcaaag gcttcgtaca
     6661 tcacttcaga gaaggttggg tgtccatgaa ttgataataa caattcatca acggttaatt
     6721 cagattccat aatagtagct gcttcgttaa tcatctcagc tgcagctgga ccaataatat
     6781 gaacacctaa gatttcgtgg tatttggcat cagcaataac tttaacaaaa ccatgtgctt
     6841 catttgaagc aatcgcgcgt ccattaccag taaagctgtt tttaccaata agcacatcac
     6901 catattgttc acgcgcttgt tcttcagtta aaccaaccat tgccacttca gggtgtgtgt
     6961 aaacagctgc tggagtgtat ttaaggttag ctttacgagt tgtgttgcca tgcatcgcat
     7021 tttctgcagc cacttcaccc atacggtaag cagcgtgagc aagcattttc gttccgttaa
     7081 cgtcacctgg cgcatagata cctggaattg atgtctcttg gtagtcatta actttgatac
     7141 ggttacgatc catttcaagg ttaagatttt ccagaccgtt catttgtgat acacgtccga
     7201 tagaaagaag agctttttca gcaacaactt cttcaccatt gttaagtttc aaagtcaatt
     7261 ggttatttgc ttcaacaatt tcagagacac caacagatgt tttgatcttc atgcctttct
     7321 tagaaaggat tttttgaagt tcaagagaga cttctttatc catagctggg ataatacggt
     7381 cagccatttc aataacggta acatccacac cgtaagatgc ccaaacaagt ccaagctcga
     7441 taccaacaac accaccgccc ataactgcta gtgattttgg catttcacgg aggtcaagga
     7501 tatcatctga cgttaagaca agtttagagt caatacctgg gatattgata cgtgatactt
     7561 tagaacctgt agcaaggata acgttgcgac ccttaatggt ttgtgaccca atggttacag
     7621 tcttgtcagg gttgacttga ccaagcccat taaagatagt gactttatta gcttttagaa
     7681 gaccttgaac gccgcctgtt aaggttttaa caacggtgtt tttaaagtca actgttttgt
     7741 ccatgtcaat agtatagtta gttgaggcaa ggttaatccc acgtcctgct gcaatcttaa
     7801 taccatcaag gatttcagca tttttaaggt aagttttggt tgggatacac cctacgttca
     7861 agcaagttcc accaaattca gatttctcaa cgatggcaat tttaccacca agttgagcac
     7921 cacgaatagc tgcataataa cctgcagggc caccaccaac aacgatgatg tcatactcat
     7981 catcagcaag tgcagctttt ggtgcagctg aagcagcttg agctggtgct tttggcactt
     8041 caagtccagc agcttctaaa tcttctgttg tacgagcaac attcacatct gaagcagctg
     8101 ggctagaaac ctcaacagat tcaccttcag caccgatata tccgataact tctgttacag
     8161 gtactgtttc acctgcttga cgtgtaattt taagaagaac acctgagtct tctgcctcaa
     8221 gttccatatt ggttttatct gacatgattt caagaagaat atcgccttca ttgacagtat
     8281 caccttcttg ttttttccac tcgatgattt caccttcttg catgtcaaca ccgagttttg
     8341 gcataataat ttcaacagcc ataatttaag ataccaagag caaggagaaa gcgattgaat
     8401 ttttgcggta agtcagaaat ctttgatttc gttgacgcac ccacgtcagg acgactagga
     8461 ctttccgaaa caaaaactga ggaaagttca gacgtctacg acgctaagat tagccaactc
     8521 ttaccatttt tatccgagct tttcgctcgg attcaattgc ttatcactca tttgcatgag
     8581 agctcctcgt tctacatcct ttctatattt ttgagaaaag ggcaccaagt ctaaacttag
     8641 caaaatccct tttattattg aatgcttttc ttgtttaaaa ctgattgatc agattaacaa
     8701 ttcaaatgga ttttccatca atttcttgag atcaaccatg aattttgcac cgttcatacc
     8761 atcaactaaa cggtggtcga tggttaggca cattgccata attggacggg aaacaatctc
     8821 accatccaca accgttggag ttgggatagt tgctccaaca ccaagaattg ctgagtttgg
     8881 ttggttaata attgggttaa atgtcttcgt accaaacatt cccaagtttg tgatagagaa
     8941 ggttgatcca gacatttcag ctgctttcaa cttaccagtt tgcgcttttt taatcacatc
     9001 ttttgaagca agcacgaaat ctgacaagca catcttgtta gccccatgga taacaggaac
     9061 gataagacca tcatcaagac ctacggcaat accaaggttc acaaagcggt gcaattcaat
     9121 atcattagca tcattgatga gggaagcatt catgtactca tgctcaggtt tcatcaaggt
     9181 tttaaccact gccataccaa tcaagtctgt aaagctgact ttaaggcctg ttttagccat
     9241 gattgggtca attaacttct tacgaagggc aatcatctct gtcatgtcaa tatcgtagtt
     9301 aagcgtaaag gtaggggcag taaggtaaga gtttgtcata cctttagaga ttgccttacg
     9361 catagctgac attggcttgt gttccacacc ttctggaaga tcaacaactt tttcttcttt
     9421 agctgctggg gcttttgctt cagctggttt agccgcttca agaatagcca tgatgtcttc
     9481 tttcatgacc ttaccgttaa agccagtacc tgatacggtt gccaaatcaa tacctttgtc
     9541 tgctgcaatt ttacgagcaa gtggtgaagc tttcggttga gcacctttaa agttttcaac
     9601 gtcttcttta tggacacgtc cttttggtcc tgttcctgga acttggccaa ggtcaatgcc
     9661 catttctgct gctgccttac gtgcagctgg agtagcacga acttttccgc catttccttg
     9721 cggaatagct gtcgctgcca cttgaggagc tggacttgcc acattttctt ttggagcaac
     9781 tgctggtcca gcgtctgctg aagcaggaac tggaatttct gttgtttttt cacttgaagc
     9841 aatagtatct actgattcac cctctgcacc aatgtaaccg ataacttcag ttacaggtac
     9901 agtatcacct gcttgacgtg taattttaag aagaacacct gagtcttctg cctcaagttc
     9961 catattggtt ttgtctgaca tgatttcaag aaggatatca ccttcattga cggtatcacc
    10021 ttcttgtttt ttccactcga tgatctcgcc ttcttgcatg tcaacgccca gctttggcat
    10081 aataatttca aatgccattt ctacatcctt tcacgatttg ataagcgcca tcaacatcct
    10141 caccttgcta cgaaaacata cgaaaggtga ggtgccgcga cacgggataa gacaactcag
    10201 ttcttctaaa aactttcaaa atagttttta gctgagacgt tactgataaa catagccttt
    10261 atcggaccac ttgccttatc ttctagttgc ctttgtttgc cattttcaca atagcagctt
    10321 tgattttctc aacatctggt aagattgctt gttcaagtac acgagcataa ggtactggaa
    10381 catcttcact tgcaagacga acgattggat gatcaaggta atcaaaggct tcactttcgg
    10441 taatcatggt tgcaatttcc ccaatgaatc caccggtctt gtaggcatcg ttgacaagca
    10501 tcaatttacc tgttttctta acagattcaa taatcaactc tttatcaagt gggatgaggg
    10561 tacgtgggtc aacaacttca acgttaatac catccgctgc tacttcttca gccgcttgaa
    10621 gaacacgctc caacatacga ccgtatgaca caattgtcaa atcagttcct tcacgtttga
    10681 tgtcaccttt gccaagtggg atatagaagt caggatcttg gttaacttct tcttttttac
    10741 cataaagcgc ttttggttcc atgaaaagaa caatgttatt atcacggata gctgatttta
    10801 ataagccttt agcatcgttg gcattaccag gagcaacaac tttgattcct ggaatgtgag
    10861 tcaaccatgc ctcaagagat tgtgagtgtt gcgcagcgga accaatacca gaacctgatg
    10921 ccacacggaa ggttacaggg gtaataaggc ctccaccgaa catgtaatta tttttagcac
    10981 cattattcac gatagcgtcc atcatgatgg taaggaaatc catgaaggta acgtcgacaa
    11041 ttggacgtag tcctgtaata gctgcaccaa tagccgcacc agagatagca gcttctgaaa
    11101 ttggggtatc tttaacacgt ttagggccaa attcttcaat cattccaaca gaagtcccaa
    11161 agtctcctcc gtaaacccct acgtcttctc ccataaggaa aatgttttcg tccttgcgca
    11221 tttcctcagt catagcaagg ttgactgctt cacgcaaagc cattaatttt gtttctgaca
    11281 tcatttttat ttctccattt attattcaaa tacgattgct tcttactgac aaggtgctct
    11341 tagtcaaccc aaacgtcttc gaaggctact gaaagttctg gatctggact gttttgtgca
    11401 aattcataag catcgtcaac ttcttttttg acttgtgctt gaatagcatc caattcgtca
    11461 tcagtggcaa tgccttcgct tgtcagataa gtacgatatt tgatcattgg atctttttct
    11521 ttccactcat ccacttcttc tttagtacgg tatttaccag catctgcagt cgagtgacca
    11581 aaccaacggt atgattctac ctcaacaata gctggaccat ttccaccacg aacatgttca
    11641 acggctttgc ccattgtttc atagacagcc atcacgtcat tgccatcttc acagtagaaa
    11701 ccaggaactc cgtaagcttc tgcacgtgtg tataagtgtg gtgtatttgt tgcattattg
    11761 atgctcattg aaattccata acggttgttg atgatgaaga aaataacagg aagtttccat
    11821 gttgcagcca tattaacaga ctcgtggaat gatccttcgt tagtagcgcc atcacctgag
    11881 aaagcaacag ctatattgtt agttcctttg tattgttgag tgagggcagc accaactgca
    11941 agagcgtaac caccaccaac gataccatta gtaccatagt ttcctttttc aaaatcagcc
    12001 aagtgcattg agccaccacg ccctttagac acaccagttg ctttaccagc aagctcagcc
    12061 atcattttat taaggtccat gtctttagcg attgattgtc catgtccacg gtgatttgaa
    12121 aaaataatgt catcgtaaga caagtgagcc accgctccaa cattggctgc ttcttcacca
    12181 actgagaagt gagtcatccc ttgtacaaat ccacgacgga cgagtttatt aatacgtgaa
    12241 tcaaattcac gaatacgttc cattttaagg aacatatcta aatgttgttc tttagaaact
    12301 gttaccattt cggcctccat attatcatat taccttttta tgataaaact ttttttcaca
    12361 atttgcaaat aattaaatcg ctttctacct taccttattt ggttttttag aaatgcaata
    12421 gtttgtgaaa ttacaaacaa aactgtacta aaaaatttaa aaatactata taacagtttc
    12481 acgttttacc gaccttgaca taaaaaacac atcattttaa aacctctttc acaccttcaa
    12541 acaagtagca aagcgataaa aataagctta gcaaaactgc taaagcttat ttttatgatt
    12601 ctatttgttc agcgatggtt tcccattctt ccataagagc ttcttgttct tgatccaatt
    12661 ggtcccattc cttttgccaa gcgattaact gcgccgtatc attggaggca tgcatgtctt
    12721 cttgaatagc accaatacgc tcttcaatgg tttcaagtct tgcttcaatc tcctcatatc
    12781 ggcgggtcaa gcgacgacgc tctttttgat tagctttttg tagctgataa tcagtggcag
    12841 aggcttcttt tgtttcctca actgtctctc ctgctgctag gcgggctaat tcttctagct
    12901 ctgccttttt ctcaaggtaa taatcatagt cacctaagta aagggttgag ccattttcgg
    12961 taatttccaa aaccttagtt gccaaacgat taatgaaata gcggtcgtgg ctgacaaaaa
    13021 gcaaggtacc gtcaaagtca atgagggcat tctctagcac ttctttactg tcaatgtcta
    13081 ggtggttagt cggctcgtcc agaacaagaa aattattgtt ttccatagat aacttcgcaa
    13141 gaagcagacg ggctttttct ccacctgaca gcatagccac cgattttttg acatcatctc
    13201 cagaaaagag aaaagcccct aatcggtttc tgatatcgac ttctggcgta gttgaaaaat
    13261 cctgccataa ttcttccaaa actgtgttgc tagacgtcaa atgtgattgg gtttggtcat
    13321 aataacctgt ttcgacattg gcaccatact tgagttgtcc tttgagcaaa ggcaattgtc
    13381 caataatcga cttgatcaag gttgatttac caataccatt tggccctaca acggcaatag
    13441 catccaactt attgatatct acattaatag gttcggacaa gacttggtct ccataaccga
    13501 tggctgcttc ctcaactctt agcacaacat ttcctgatgg tttttctgca tgaaatgtca
    13561 tatgagcaga tttacgccca cctgtgggtt tgtccaaacg ctctattttt tccaattgct
    13621 tacgcctagc ctgagccctt tttgtggtag aggccctgac aatgtttttt tgaacaaaat
    13681 cttctagctt ggcaatttct ttttgttgtt tatcgaactg tttttcttcg gctactagtt
    13741 tttcagcttt taaggccata aaacgagaat agttaccact ataccgatct aagccgttag
    13801 gcgtcaaatc taaagtcacg gtcgccactt tgtccagaaa ataacggtcg tgactcacaa
    13861 tgattagagc tccttgatag ttggccaagt agttctctaa ccaggcaatg gtttcaatat
    13921 ctaagtggtt tgttggctca tcaaggacca atagttctga cttttctaaa agcattttag
    13981 ccaaggccag acgagtattt tgaccccctg acagctcagc gatagtcatc tgccacatgc
    14041 tctcatcaaa cttaaaccca tttaaaatgg ctttgatgtc agattcatac gtaaagccgc
    14101 cttgctgacg aaaatgctct gtaagcagat cataatctgt catcagtcgg gttaaaactt
    14161 gacctgaaac ggttgccata tccatttcca tctgacgcag gcgcttttca tcttggcgta
    14221 atgcttcgaa aaccttgagc atttcctcat atattgtttg atctgattca aaacggctat
    14281 tttgagccag ataagacaag gttaggtctt ttttagtatt aacttctccg ctcgttgggg
    14341 tttcttcccc aaccaataat tttagtaaag tcgacttgcc agctccatta ggaccaacca
    14401 aggcaatgcg gtcacgttca tcaacttgta gtgatatgtt ttgaaaaaga acatctcctg
    14461 agaaagagcg ttctaacttg tttccttgta aaataatcat actttcattc taacaaaaaa
    14521 ataccctttt gaggacattt tcagcctagt ttccaaaaga aatcgaactc tttttaagtc
    14581 gttgattacc ttctttagcg ctaacaagga aagtggaatg actattacca ctcccatgac
    14641 gcgtctttgc agatggcttt tgtgttaaga tatcaaaaat ttgcaccact tgctcagtaa
    14701 ctatcgagat cttattggct aaaacggaat cactgtgatt agaatagtac atcagcccta
    14761 taataataat cgttactaat aagatagttt gtaaaaaccc tttgataagg tgccaaaaca
    14821 ttttcactat tagcttaggc agccacaaaa tgatcgccag taattgtttc atttgtcacc
    14881 tcatgttgta ctaggaaatg gttaagacaa cctttcttct ttttggtagt ttatcggtaa
    14941 ccactctaac aggacttcta attgctggtt ccaatagtac cactcatgtt taccatgacc
    15001 tttatgataa tcaatgtcaa gaccttgagc ctgaaaatca gcaatagctt tttcattagt
    15061 ggcaaaaaga aaatcctcat agccacacca agcataaaat ttggtttttc catcactttc
    15121 tgccaccata tttttaaggt aatgcttgtc aaggtcaggg tcatcaaact ggccaaaaac
    15181 gccctgccaa taggctaatt ctcccagttt tccctcaagc aaggtctcag gagaaaaatc
    15241 caaagcgcct gaaaacgatg ccgcataaga aaaccgattg cttttgagtg cccacttaaa
    15301 ggcaccatag ccacccatag agagcccagc aacaaaggtt ttttctcgtt tctgggtcat
    15361 attaggaaag aaagctgcta agacctgcgg taactcttgt gacaacgccc gataatagtt
    15421 aagtccataa gccgtgtctg tataccaacc taggtctgtc gaaggcataa cgactatcaa
    15481 attggtatgc cgcaacaagc gttcaatagc tgttcgtttt tgccaagagt tttcattgcc
    15541 tcccatacca tgtaacaaat ataaaacagg aatatcctta tccccttgat cttttttagg
    15601 gatttcagac tggtcaggat aaatcacatt taccttacgc tccattccta aaactaccga
    15661 atggtattca attgcaatac ttgccattca ttacactcct ttataccaaa ataatcctat
    15721 cacgtaaatc tactgtattt ttgggtgaac agtttcctgg tttaccagtt gttaaaatca
    15781 aagaaggtag gcagtttttg cctaaccttc ctattttcac taacgaactt ccatcacaac
    15841 cggcagaatc gctgggcgcc ttttggtttg ttcaaataag aatttagaca aatcatctct
    15901 gacattccct tttaattcgc cccagtcaaa ggtatctttt tttaagtaat tgccaacagt
    15961 tgtattaact aattctgcac tttctcttaa aatatcatga cttttcttga catacacaaa
    16021 tccacgcgta ttaactttag ccttagaaat aatgcgtttt tcttttttag aaacagtaat
    16081 agcaacaatg aaaataccat cttctgacaa gactttacgg tctcgtaaaa cgatattacc
    16141 cacatcaccg atagcattac cgtcaatcat gacatcactg gcaggtacac ctccttcatg
    16201 gagaaaacct tcgtcattaa gcaccatgat atcgccacgc ttcagaatat gaatattctc
    16261 tggaaaaata ccaacttctt cagctagctt tgcatgggca gctaaatcac gatattctcc
    16321 ttgtactgga aaaagatact ggggttttaa caaattcatc aatagttgca aatcacgccc
    16381 attagcatgg cctgaaacac gtaagttttg agtgattaat ttaacagaac caccggcctt
    16441 gtaaatcaag ttttcaacac gcgccaccat agcttcttta gccgtacttg gtgttgttac
    16501 aatataaaca aggtctcctt ctttgatttg aacataacgg tgacgtcccg ctgccatttt
    16561 ttgtaagcta ttaattggct cacccatacg accagcttca aggatgatta attcatggtc
    16621 ttcgaattta gacatatcct taggtttaat taaaagacgc tcatccgtaa tcattaattt
    16681 ttctagacgt aaagctgttc tgacaatatt ttctgcatct gttccggtta aaacaacacg
    16741 acgcccatgg gcagtggcgg aatcaaatac ttgctgaatc cgaacaagat ttgaggctac
    16801 tgcagctaca atgacacggc catcagcatc agagataaca ctgtccattt cttcaccaac
    16861 ttcagattcg ctggcaatct gatcgttgct tgtcgcattg actgaatctg ataaaagagc
    16921 tagaacccct tctttcccaa tctcagctaa acgtagcaaa tccgtctgat aaccttctct
    16981 tgctgcttga tcaaatttga agtcaccggt ataaataatg tttcctttat ctgttccaat
    17041 aacaataccc atactttctg ggattgaatg cgttgttcta aagaacgaaa ctaggccatc
    17101 tttaaattca atttcagtat cactgtctac cacatgaaaa ttattaaatt tcttagtgct
    17161 gttattactt ttcacaaata gcttggctaa ttcaatagtc aattcagatc caaatacagg
    17221 agctgatact tcagctaaca aataaggaag ggctccaatg gcgtcagcat ggccatgact
    17281 caagaaaatc ccttgtactt tacctttgtt ttcaattaca taatctaaat tggggatgac
    17341 taaatctacc ccaagttgct cattttcagg gtacttgagc cctgcgtcta aaataaacat
    17401 agaatcgtta atttctacca aatagaagtt ttttccgtat tcacggaccc ctcccaaggc
    17461 aatcatttta atatcagtca tgatactcct ttaaatattt tattattaca aacggtcctt
    17521 acaacgtaag tcgaattact gttatgtagc tatgctacta tcttctatag tatatcatat
    17581 ttgaggcgtt ttttctctaa atagccttga tactttcaga ttattttcag tatggaaatc
    17641 aaaaaatcac tgacttatta ttagtcaatg atttagatcg ttgattaatc aaatagttgg
    17701 taatagtcag taatcgtcag ttgttctttt tccatttgat ttaaatcttt aataatgttt
    17761 ccatctttca ttacaatcag acgattccca taagttaatg catcttccat atggtgggta
    17821 atcatcaaag ctgttaagcc atcttttgtc acaaactcat ctgtcaattg cattaaagat
    17881 tgactcgtct tagggtcaag agcagccgta tgttcatcta ataataagag agctggcttt
    17941 ttcaacgttg ccattaataa acttaaagct tgtctttgtc caccagacaa aagacctgct
    18001 ggtgtttcta aatgcttttc taggccattt cctgttcgtt taaccaaatc ctcaaaacta
    18061 gctaagtgct ctgttatttt tcgagaagcc aaggagcgtt tttctcctct ttgacgtgcg
    18121 attaagagat tttcagccac tgtcatccga ggggctgttc ccattttgga atcttgaaag
    18181 actcgcgata ggtaaagagc tcttttttct gctggccaat gcgtcacatc ctgaccaaga
    18241 atcctaattt gcccccgtgt caaacttagc gttcctgcaa ttacattaaa aagggttgat
    18301 tttccagctc catttcctcc taaaatagtt aaaaaatcat gttcatagat agttagtgtc
    18361 acattgtcca atattgtttt agcatcttca aaaccattat ctacgtcaac tgttgcattg
    18421 atcaattcaa taatttttgt catcgtgtta acctcactcc cttaaagaag cgctctttta
    18481 ccactggtat catgaggcac agtgctaaaa caagagcact aattaatttc aaataacttg
    18541 tattaaaacc tagcgtaata actactgaaa ttaaaaattg ataaagaatc gagcctatta
    18601 caatagcaat caaacgttct aaaagagtca gtccagttga gtatagtact tcaccaacaa
    18661 ttatgctcgc caaaccaata acaataactc caatcccttt tgagacatct gcatagccat
    18721 cttgttggct cactagagca cctgataaag ctattaaact attagaaacc acaagtccca
    18781 taacttccat ccaatccgtg ttaataccaa aactcttagc catatcttta ttgtctcccg
    18841 tagcgatata agcttgacct agattagtgt aaaggaaata gatcaaaacg ctaataacaa
    18901 tcacaacagt tatcagacct gttaaaaggc tatttaaatc aattgaaaaa ggtaaacaat
    18961 cctgaatacg tttatgatca tgtaaaccca agtttgctct tcccattacc attagcataa
    19021 tggagttaca tgacgtcatc accaaaattc ccgctaaaag tgttggcatt tttcctttcg
    19081 tatataacag ccctgttaaa aaacctgcta aagcacctga taacatacca agtaaggttg
    19141 atagaaatgg attccaccct aagctaatag cggtgaccgc aacagctcct cctaaaggaa
    19201 aagaaccttc tgtagtcatg tcagggaagt ttaaaatcct aaaggtcagg tagattccta
    19261 atcctaaaac tccccatata aggccttgtg aaactgatga aataatcata tttttcctca
    19321 tatttatatt agcgggtaac tctcgccagt ataaaccctt tctttacatt aatttttaga
    19381 tttgttttat acttagttca gcaccgtggc ttagtctttt ttttcaatca ctttgacgaa
    19441 actaagacag aaaacaaaaa agtcgagaat catgtttaaa cttactcaaa aggcaagatt
    19501 ttttacgtgt ctaaaataga gcattttaaa actaaaaatg ataaaaataa ccgttacatc
    19561 ctcaaaacga ttgtctaact tggcatccaa attcatctca gtgagggtgt tttttatggt
    19621 tttttcttga ccttttatat cacatgtcat tgtattaatt ctcaataaaa gaagctgaag
    19681 gaaaatcttc ttttttaata gcaataccta attctgctgc tacattctta ttaattagcg
    19741 gttgaccaat atccacaact ttgacaggaa catctacaac acgctttcct ttgattaatt
    19801 ttaacacctg attagctgtt tgaactccca aatcgtactg attttgagaa atagctgcca
    19861 aaccaccttt ttcaaccatt cgatcaacgc tagtgtagac aggaatcttt gcagccttgc
    19921 tggttgtcat gactgatgaa aatgcacttg ctatagtatt gtcttggggg ataaataccg
    19981 cgtctacttt acctaacata actgacatcg tggcaggaac ttcattagta gaaggaaccg
    20041 cgtatgaaat aacttggtag ccctttttgc gagctaaccg cctaaactct ttaacttgag
    20101 ataaggaatt atcctcatta cttgcatata aaattccaac tgttttgaca tgtggtgtca
    20161 gctttttcat taaatcaatt gtctgtttaa caggcacttt attagataga ccagttacat
    20221 tagcttcagg ttgatccaat tgcatcacta atctagatcc tactggatca gatactgctg
    20281 acatgacaac tgggatatcc ttgctggtgg ctgctaagcc ttgcgccgcc ggggttgcga
    20341 taccaatcac gatatcactt cctgattgca ctaattgcct actcattgtt tggattttac
    20401 tctgatcccc ctcagcattc ataaccttaa tcactacatt ttgtttttga ggcatctgct
    20461 ttttgagttg atcttcaata cctcgcttaa tatcatcaag agcctcatga gtaacgaatt
    20521 gtaagatgcc aattgtaatg ttttgttggt tagccaaatc acggttttcc tttgatactc
    20581 cttttgataa tagagagcct atcactatta cagttaaaac aaggagggta gctattaaac
    20641 tcttgttttt catctgaatt cctcacattt catgtcaatc tctttaaaac gttgatgatg
    20701 agaatacttc tcaaaagaaa aagcacttag taatgattac taggcgctct tgtgaaatcc
    20761 aatgtcagcg catagatatc tttttagaag tactctaaaa gtcgtatcta tgccaattca
    20821 taaacacaac ttatctatgc cagctttgcc aatttgctaa gtgttgtgtt tttaccatca
    20881 ttgacttcct ttctcgtttt tttaacatta taacctaaat caaaaaaatt gcaagctctt
    20941 tcgtccatat ttttagtcaa gtatttgtgg tataatgaag agattagaat tcagaaagga
    21001 gaaaccatgt cttttgacgg ctttttctta caccatttaa ccaacgaatt aaaagagaac
    21061 ttgctttacg gtcgtatcca aaaggtcaat caaccctttg aacgggaact tgtcttaacc
    21121 attcgtaacc atcgcaagaa ttataaattg ctattgtctg cccatcctgt ttttggaagg
    21181 gtacaaatca cccaagcaga ctttcaaaat cctcaggtac ctaatacctt taccatgatt
    21241 atgcgtaaat acctccaggg tgcagtcatt gagcagttag aacagattga taatgaccgc
    21301 attatcgaaa tcaaggtatc taataaaaat gaaatcggag atgctattca ggcaacccta
    21361 atcattgaaa tcatggggaa gcacagtaat atcatcttgg tggaccgtgc agaaaataaa
    21421 atcatcgaat ccattaaaca tgttggtttt tcacaaaact cttaccgcac cattttacct
    21481 ggctctacct acattgagcc tcctaaaaca gctgctgtta atccttttac aattacagat
    21541 gtgcctctat ttgaaatcct ccaaacacaa gaactaacag ttaaaagctt gcaacagcat
    21601 tttcaaggtt tggggcgaga caccgcaaaa gagttggccg agcttctgac cacagacaaa
    21661 ttaaaacggt tccgtgagtt ttttgctaga cctactcagg caaatctgac cacagcttct
    21721 tttgcgcctg ttcttttctc agatagtcat gcgacgtttg agactttgtc tgacatgctt
    21781 gaccactttt atcaagacaa ggctgagcgt gaccgtatca atcagcaagc cagtgatttg
    21841 atccaccgtg tgcaaactga actggacaaa aatcgcaaca agctaagtaa gcaagaagct
    21901 gagctgctag ccactgaaaa tgctgagttg ttccgtcaaa aaggagaatt attgaccacc
    21961 tacctcagtc tggtcccaaa caaccaagac tctgtgattt tagacaatta ttatacaggg
    22021 gaaaaaattg agattgcctt agacaaggct ctgacaccaa atcaaaatgc tcaacgttat
    22081 tttaaaaaat accaaaagct aaaagaagct gtcaaacact tatctggatt gattgccgat
    22141 accaagcaaa gtattactta ctttgagagc gtagactaca atttatctca agcaagtatc
    22201 gacgacatcg aagatattcg cgaagaatta tatcaggctg gctttttgaa aagtcgccaa
    22261 agagacaaac gccataaacg taaaaagcct gagcagtacc tggcttcaga tggaacaacc
    22321 attctcatgg tgggacgcaa taacttgcaa aatgaagaac tcacctttaa aatggctaaa
    22381 aaaggagaac tctggttcca tgctaaggat atccctggaa gtcatgtcat catcaaagac
    22441 aatcttgacc caagtgacga ggttaaaact gatgctgccg aactagctgc ttattattcg
    22501 aaagctaggt tatcaaatct cgttcaggtc gatatgattg aagctaaaaa attacacaaa
    22561 cctagtggcg ccaagccagg atttgtgact tatactggtc aaaaaacttt acgggttact
    22621 cctgaccaag ccaaaattct ctctatgaaa ttatcctgat aagcaactaa aaaacagctc
    22681 tcctaggaaa gttatttccc ctaggagaac tgttttttta ttctgattgt tggtgcttga
    22741 tatggtcaag aaagtcttgc atattcacat catcaggcac ttgtctgagg taagcggtag
    22801 ccatttggat agccttgtga aactggccaa actcccttaa taaataggca taatcctgta
    22861 aaaactctgg attttctgcc aaatcagccg aaatctcatt ataaagagcc aaggcaactt
    22921 cttcttgttc caaggcgtgg taagcctttg caatggtcca tttggttaag acattatcaa
    22981 tcgtctcacg attaagagca ataacctcct caaaacgctc ggcatcaaaa taaagggtca
    23041 ctaaacgcat gagaatctct tcgtcatcaa cagctacttc ctttgcttga agcaggtaat
    23101 tttcagcatt ctgcctatcg tgtaattcat aagaaagctg cgatgctagt aataataatt
    23161 gactgtcaaa tgcattttta cgtaggccct gttgaaccaa gcgcaaagct tctgacgtct
    23221 tatgctcttc atgaagactc aaagcataac catattcata acctggataa tctggattga
    23281 tggtttccaa ctgtttaaag taaagattgg ctttttgata attttcttgg tcatacatga
    23341 gggtcgccaa ttcaaaaaca gtttcatctt cgtattcaat ggccacggcc ttttccaaga
    23401 attcgatggc tgcctcaaat ttccctaagc tagcataggc tcgtccaatg cgttgataag
    23461 tggaaatacc tgtcaattca aggatttgac gattgtctaa ctgtgcatag taatcaatag
    23521 cttctttaaa atgctgcaag gacatgtcaa tttctgccaa accaaaaatg accaatggct
    23581 ctgggctgat gcccactgct tgaagcaatt tttcacgcgc cacctctgtc agtccttcca
    23641 tgtcatagag gtcagccatt actaagagag ctgacaaata attggggcta tcttttgaaa
    23701 ccttatccaa atacaagaaa gcttcttcaa tggcgtcgtc ttctgctgca atctgagcta
    23761 aatttatatt gagttctggg taatcatcag ctaactgcaa ataaatacgc ttggcatgtg
    23821 gcaaaaaacc tatactttct aagtattctc ctaaggcgat taaactatct gcatcatctt
    23881 cttttaaagc tttttggaaa tatttttcag cgtgcgctag gtcttgctga tccaaggaag
    23941 caatcatttt ttcactattt aacatggtcc ttaacctcct caatcaccaa tacttcttcg
    24001 tcatacaaac cgctaacttt tttgtaccag tcaaataatt ctttaatcac aactttgaca
    24061 gaagcgtaga ttgggatgac aaggaaaaca ccccagacac caaacataga gcctgcagtc
    24121 aataacaaga acataatggt aataggatga atgctcagct tatttcccaa aacaagcggt
    24181 gctacaaaac gcccttcgat ggtttgctca atcataaaga taacaagcac cttaaccaac
    24241 ataaatgggc cttgtaccat agccataatc acaacaggta tcatggcaag gaaacttcct
    24301 aagtaaggta tcatgttaag aaagccagca ataatcccaa aagtgatggc atatttcagt
    24361 cccacaaggc taaacatgat agaaaacata aagccgacaa caattgcaac cgtaacctgc
    24421 ccttgcacat agcctgataa ttgcttgtta atatctccca agactcgaac cataggcacc
    24481 cgtaatttga gaggtaagac attcacaagg ccatttttca tgtggctact gtctcgtaaa
    24541 aaataaaaga gaataaaggg cgaaataata atagccacgg taatccgagc aatggcacca
    24601 gcaaaattac cagcccagtc aatagcattt tttgaaaaac tttgcgcgta atccaaagcc
    24661 ttttgacttg tatgggtaag catgtcttgg agttggggct tatagctgac taaccattca
    24721 ttttcaagaa gtttattggc ttcttcattg accttgccca cgtatttggg cagatcctca
    24781 ataaaactgg taagttgggt ctgtaccatg ggaacaaagc ctgaaatcgc ccagacaaaa
    24841 agtaaggtaa tcaaaccaaa aacaataaaa atagaggtgg tacgattcgg gcccaagtga
    24901 ttaataaggt caactaaggg tttggtcaga taatacagaa tggtcgaaat aaccaagggc
    24961 aacatgatga ccgcaaaaaa agaaatcact ggcataaata aaaaagagat tttagtaaaa
    25021 acaaaaatcg ttaaaaaggc taaaagtgtt attactaaag ccatggtagc ttgattattt
    25081 aaaaaccatt tgtaaaataa gctatctgtt ctagactttt caggtattaa gggcattaag
    25141 atagggttcc tttctttggg aaaatgatag gttttactgg taacctgggt aaatcaataa
    25201 aaggtcacag atttatccat gagttggttg ttttggtcat aggtaaattt ggcagagaaa
    25261 aaagaacggt cttctaaaat ccatttaaaa atgtcataat caccttccca agttggcttt
    25321 tccaacactt gatcgtaagg tacccattcc aaggttcctt ctcgtgactc ttcatctgaa
    25381 atgaggtctc cttcaaatcc tgttacttta aaaacatagg tataccagtc atgaccaggg
    25441 gtaaattctg gaaaagtaat gattccttta aaggccatct ctgtaacagt caaatgggtt
    25501 tcctctaaaa tctcacgacg ggcacactca tctggggtct caccagcctc gagcttaccc
    25561 ccaactgaaa tccacttgcc tttgtggaca tcattttctt ttttattgcg gtgtaataat
    25621 agcaaagaat cgccattatc aatgtaacaa atggttgcta attgagtcat ctatctcttt
    25681 cctttccatc tttgtttcta ttataccaat ctctagaaaa tatgctatca ttttatgagt
    25741 caaagtgtga atacttagtc acgctcaaaa ccttctaaag atgccttaaa aaagtgtaga
    25801 taagagccta caatgaaaaa catcaattta aactatcaaa agaaccttgt catatcaatt
    25861 atcgttgata tgacaaggtt ctttaatgtt ttatttaatc acttcttgag ttttagcata
    25921 cttggcttct acagcatctt tttctgcttt ccaccatgtc tcattttctg tgtaccactt
    25981 aatagtttct tccaaacctt ctgaaaagtt tgtaaattgt ggttcccagc cgagttcttc
    26041 acgcaatttt gtagaatcaa tagcgtaacg aagatcgtga ccagcacggt cagttacgtg
    26101 atcataagcg tcttttggtt gacccatttt ctcaaggata agctcaagaa cttccttgtt
    26161 gtttttctcg ccgtcggcac caataaggta tgtttcaccg atacgaccct tagtcaaaat
    26221 agcccatact cctgtagaat gatcattagt atgaatccag tcacggacgt ttttaccttc
    26281 accataaagt tttggtttga ttcctgccaa gatatttgta atttgacgtg gaatgaattt
    26341 ttcgatgtgt tggtatggtc catagttatt tgaacagttc gaaatggtcg ctttcacacc
    26401 gaaggaacgt acccatgcct taacgataag gtcagaagct gccttagttg atgagtaagg
    26461 tgatgatgga ttgtattttg tttcagcagt gaatttttca cctggtcctt caccctgtcc
    26521 tggaaggtct tcacgaagtg gaagatctcc ataaacttca tcagttgaca cgtggtggaa
    26581 acggatatcg tatttacgag ctgcttcaag caaagtgtaa gttccgataa agtttgtatg
    26641 gataaatgga cttggatctt ccaatgagtt atcgttgtgg ctctcagccg catagtgaac
    26701 aataacatcc gttttggcag ccaatttatc taccaattca gcgtcagcga tatcaccaac
    26761 aactaactca acacgatcac caagaatagc ttcaatgtta gcacgattac ctgcatatgt
    26821 caatttatca aggacagtta catgaacatc tgggtggtta ttgtagacat agtgcacaaa
    26881 gttagatccg atgaaaccag ctccaccagt tacgataata tttttataca taagatataa
    26941 agggaccttt cgtttccgaa aaaatctatt aaaagtaatg actctaacaa agacgtttta
    27001 ggcctaagaa aacatcgctt tctaagattt aagtcccatg ttcagctcaa agagagaaac
    27061 tgaactttcc tttcatcatt tttagttgtt acttgttacc tgaagtagag aagaataact
    27121 cccctcatat ccacctcaaa tgattaatcc caatcatctg agtatgttgt ttccctatag
    27181 gtcttttggt ttcagtggtt tgacatcact aagaagagga tggtttttat ctgcttcaga
    27241 aacttctgca gctgctagat tttcccaagt aatcccaaga cttgggtcag catagtttac
    27301 aaaagcatac ttaggtttca agtcaagagc ccagtagtca ttgacaagat aactgtaaga
    27361 gacagtctct gaaagaactt gaaagccatt agcaacccct ctaggaacaa acattccttt
    27421 tgaagcatca atcactgtct ggtagacgtt tccaaaagtc tctccctcac gaagatcaac
    27481 ccaagctcct aaaaccttgc catcatcagc aactgagatg tatttatccc aaggttcagc
    27541 atgaagtcca cgcagcacat gctgacgtga aaaagaaacg ttgttttgta gttttccttc
    27601 ctcaaaaaaa cgttctggaa agccaattgg caacattttt tctttttgga aattttcttt
    27661 aaaccagcca cgattatccc cacgaacagg aatatcaaac tctagtaagc ctgggatttc
    27721 tttgatttct cggcaagcca atggtttgtc aaaaaaagtt tctgtcatta agcttctcca
    27781 atcaaacgaa gcaagtattg cccgtattcg tttttcttta aagattgcgc caatttatgg
    27841 acatcttctt tactgatata gcccatgcga taggcaattt cttccagatt tgccacttga
    27901 gcgttttgca agcgttgaac agtttcaata tattgagccg cttcaagtag actttcatgc
    27961 gttccagtgt ctaaccatgc aaaaccacgc cccatcaatt caacggagag gtcaccacgt
    28021 tccaagtaag ccttgttgac gtctgtaatt tccaactcac cacgcgcgct tggtttaatg
    28081 tttttagcaa tctctaccac gtcattgtca tagaaataaa gtccagtcac tgcaaagtga
    28141 gacttaggca cttctggttt ttcttcgata gagatagcat tcatgttctc atcaaattcc
    28201 accactccaa agcgctcagg gtctttcact tgataaccaa aaacggtggc tcctttttct
    28261 ttggcagctg ccttttgaag cattttagtg aggccattac cgtggtaaat attatctcct
    28321 aaaataagag caacacggtc atcaccgata aactcctctc caataatgaa agcttgtgct
    28381 aaaccatctg ggcttggttg ttccttatag gataagctaa tgccaaattc tgacccgtct
    28441 cctagcaatt cttcgaaacg aggaagatct tggggagttg aaatgattaa aacatcttta
    28501 atccctgcca acatcaaggt tgacaatggg taataaatca ttggtttgtc ataaatgggc
    28561 atcaactgtt tagatgcggc acgggtcagt ggatacaagc gtgtccctga acctcctgca
    28621 agaataatac ctttcatagt agtgctccta ttcttctata taccctatca ttctatcatt
    28681 ttttgaaagc agtgtcacgc tttaccagta gaccgcaaaa ggtaaggctc aggagaatag
    28741 tcgctaggcg tcaaaagtcc ttcgtgtcct agcaataatt gagctaattc atagccaatc
    28801 aaaggtccaa ctgttaatcc cgatgaccct aaaccactag ctgtataaag gttcttcaat
    28861 cctgaaacct gaccataaaa aggagaataa tcactggtat aagctcttat tcctacacga
    28921 cacgtttttt gagtagcttc ttttaataac ggcaaataag taagagcttg catttcaagt
    28981 ttttctaaaa cctgccaatc aggctccaaa tcataacctt tatcattttc gtgactcgta
    29041 ccaacagaaa tcttaccttg gttaaaagga ataagatcta tttcaccttc tggcataaca
    29101 actggataag tgtcgctgat gatgtgatga acgtcataat ccaagagttg tcccttttgt
    29161 ggtcgcacat caacctgata gcctaaaggc ctcaataagt caggtagcca tgctcctgct
    29221 gccagaatga cctgatcaaa ataatgacca gcaatctcat aaccacttcc ttgctgtttc
    29281 agagtgactt tttggcgaat aactggataa ccactagcct tgagtaatgt ctcacataaa
    29341 gcggctccct ccactctagc agctcctgaa gcgtacagac agttatcaaa gccaatcaat
    29401 cctttaaaat cattccccag ttctttacga tttttaacgg ctaactcacc aataatcggt
    29461 gattctacct ttcttgctaa cgctaattca taaagatctc ttagtttttc ttcctgcttt
    29521 tttaaaacat agatgccatt ttgctgataa aaatctgttg caaaaccatc ttctttgagg
    29581 tcattgatta attgctgata aaagtccgct cctaaacgag ccatgcggta ccaaacttta
    29641 ttgcgacgct ttgaaaacca aggactaata ataccagcag ctgccttagt ggcttgtcct
    29701 tgaccatggt caaaaatggt aacttctttt tgaccacttt gttgtaaata ataagcggcg
    29761 gttgatccta caatccccgc cccaataatg gctattttca ttcctggtat ccttttatct
    29821 tattttaaga ccttaacagc ctataaatgg ctaaatggat tggtcgaggc tttgcttgaa
    29881 ataatgctga catcccaacc gttctcttct ttccaccctt ggagtttctc ctttaatttc
    29941 tccgtaaaaa gcacctcaat atgatgcccc gggtcaaccg caaatagtcc ttctgtcagc
    30001 atctcctgtg ctgtatggta atagatatcc cctgtaatat aaacgtctgc gcctttttgc
    30061 acagcatcct gataaaattc accaccactt ccgccacaga tagcaatctt tgaaatcaaa
    30121 gggttttcct tatcgtaacg gataagtcgc acggtatcta aatcaaacac ccttttgacc
    30181 ttacttgcta actcttctaa cgcttgttct ttaactgttc caatacgacc aatgccaaag
    30241 ccttctttgg tttcagacaa gtaagttgct tccttgattt ctagcaggtc acaaaaccaa
    30301 tcatttagcc cacctggtac aatgtcaata ttggtatggc tcacatagac ggaaatgtca
    30361 tgcttgacca aatctagaag gatatcacgc tgaggggatg aaaccagatc tttaagcggt
    30421 ttaaagatgg gagcatgctt agttataatg agatcaactt catttttgat ggcttctgct
    30481 actgttgact ctcggatatc taaagtaatc ataaccttac gaatgtcttt atccaatgaa
    30541 cccatttgaa gacctttaac gtctccttcc atagataaat caagtgggca aaaggcttca
    30601 taggcatcaa ttagggtttt agctttcatg aacaatcacc tcttttattt gttgtatttt
    30661 tgtcaagaga agctgacgct cttgagtttt ttcttctggg atgcaggaca aggcataagc
    30721 taacttatcc atttctcgtt gccacttctc tttaaaaacg actgattttt cctgagaaag
    30781 gtatggacca aaacgcaatt ctgtcgcgga taaggccttt tcaccatgct cagcaacgat
    30841 gatttcataa tatttgtcat tttcagccat gatggtttct gcaacaattt taaaagcgtt
    30901 aaccgataac caagcccgta aatcatcctc acgattgttg ggttgtaaaa ccagacgttc
    30961 aatgccctgc aatttttcct tacctgcctc taaaatatca gcaatcaaac ggccacccat
    31021 gccacaaatg gtaatggctg tcacatcatc agcttcttca aaggctgcaa ggccattggc
    31081 taagcgcacc tgaatatgct cggcaaggcc agactgtgtg acatttttaa gagcagattc
    31141 gtagggaccc ctaaccactt cgccagcaat ggctgctgaa atctgattag tttctaccaa
    31201 aaatatggga aggtaggcat gatcactgcc aacatctaat aatttgacac cttttggaac
    31261 ataggccgca acttgggcca aacggtttga taattgacta tccatcttga tttctttcta
    31321 aaacgtaatg gttctattat agcaaaatta cctccataaa aaaagaaaac caaaggctag
    31381 gctttcgatt ttccataagt atcttgatag tacttgcagt aagactgaac gggacaaatc
    31441 tcacattttg gttttttggc taaacaatgg tagcgtccaa aaaaaatcaa tcgatggtga
    31501 gtaataatcc aatctttctt tggaatttta gccatcaaat ctgcttcaat ctgcttgaca
    31561 tcggcatctg gcgatgaaat attgagcctc tttgagactc tagccacatg agtatcaacc
    31621 gcaatagctg gaacaccata aacctctgcc agcaccacat tggctgtttt tcggccaaca
    31681 cccggtaaac tttcaagctc tttgtgggtt tttggcactt gacccttaaa atcatcacga
    31741 atagcctgag cagttttaat gatatttttg gccttatttt tatagagtcc aatcgttctc
    31801 agagcatttt caacatcaga aagttcagca aaagctaagt cttctatttc tggataagac
    31861 tgccatagtc caggagttac cttattgacg gccttatctg tcgtttgagc tgataaaatc
    31921 accgcaatca acaattgaaa aggtgtttcc cagtctaact ctcccttagc ctcaggaaac
    31981 atttgaccaa tgatggtcag gacttttgct aatcttgctt ttccaattct catgaatcac
    32041 tccataaatt catcgcagat agaaaatcct ctgatatcgt cacttgagaa agatcttcgc
    32101 cttctctaac acgtctacgt tcttctactt gtcgaagatt aacaatacct tcttttcgcc
    32161 aattacgtaa gatagcctgg atatatttcc aatttgtttt gccgttgaag acagcttctt
    32221 ttaaagcttc tcgtatcaaa tcaggatctg ttttatcatc acgaagcgtt ttttcaagat
    32281 cttctaactc aaaaggactc aagaatcgtc ctagttcacg ttcaaattca tcaactaatc
    32341 gtttgaaatg atttggtgtt tcttgtttgt caatttcagt tgccgtttga ctgacaaaca
    32401 attgatctaa cttggctaac acaggactcg catcaaaaat aatttcaatt tctccagtaa
    32461 gttcaattgt ccgcatatct aaaagccctt gattagtcaa agatgaaatg attttattaa
    32521 tatcagcaac tgattttcca agcgcatgag caatttgact gggtgctaag tcgtctcgct
    32581 ttgtcgtatt ttgaagatag aaaaactgcc agaccaaaaa atcatctgag ctcttaaaaa
    32641 gatctttgta atgaaaaagc aaggcacttg ggataaccaa attacctgac ttataatgtt
    32701 ctaaaaaact cataatccct caatcaaaag gctaaaatcg aatctatagc ctttactact
    32761 ttaaatcccc taatcagatg ctgattaatg tgtttaccca ggaaactgca ttaagacttt
    32821 atagtcatag ttcctaatag catggcgtcc atttaaacct tctaattcaa taaggaaggc
    32881 acaaccagct acaataccac caagtttttc aatcatttca atagttgctt ttactgtacc
    32941 gccagtcgct agtaaatcat cgacaatcaa aacacgttgt ccaggcttaa tagcatctgc
    33001 atgcatagtt aaagtatcaa gaccatattc tttttcgtaa tcggcagaga caacatctct
    33061 tggcagtttt cctggtttac gaacgggagc aaaaccaatt cctaactcaa ctgcaacagg
    33121 acaaccaatg ataaaaccac gcgcttcagg tccaacaacc atatcaatat ctttatcaca
    33181 agcgtactgt gctatttcac gaatagcata gctatatgct ttaccgtcag ccattaaagg
    33241 tgagatatct cgaaacgtaa taccagcttt agggtaatct ttgatcgatg caatgtaatt
    33301 tgttaaatcc atagttaatt ctttctagtg gcaaattgcc aaatataaaa tactcttcat
    33361 tatatcacga aacatatttt tcgtaaaata agacttctga cattattgtt tatctgatac
    33421 ttcattcgta actacctcaa taactactgt caactgcttt acttattgat cgtttgattt
    33481 caccaaataa tcatacatct ctttcgggct agccaaagcc atgatttctt gaaatttcac
    33541 caattctttt aagtcttggt aaatgtggct ctctgaaatc tctcgttttt gcgcttgggg
    33601 gttaaccgtc atcagcccat cgtcaatcgt cacaaaagac aactcttcaa aaatttgaat
    33661 caacttaatc agtaaaagtt tttctatgtt taaatagtga ctcaactctg tcaatttatg
    33721 tcgtaaatca aattcaggaa actgatagat ggttttgtag agcttggcaa actgctctct
    33781 gctgccaaat cctgttaaat aataaggatg tttcatctga tttttaaaat agatggcgtc
    33841 aaaatccttg tgaacaaact ggttgcgcca ggttttgaaa tcttcaggga tgtcattaat
    33901 cagaatgact ctggcatttg ggtcttcttc aatagtcgga ataccttcag gaactttggc
    33961 tgtttttgtc cttaagtcaa gaagttgcac tccgtcaaca cgagcatcta ctaacatgaa
    34021 ttggagactc gtgttcccat tccaatggtt gacagataag gtaactgcta attctaaacc
    34081 agtagcttgt cggaactctt gcagttgact tccttgacca aaagccagta cgtcaaagga
    34141 agctttgccc tgactgactt taaatttaag atggctctgg tcttgcccta tggtacgcgc
    34201 ttgactaacc cttatgtcct tgacataaaa gaccggcttt tgatgatcca tcccataagg
    34261 ggctaattta tctaaactct ttaaaatatc aaggctaagg tcgtccaaag acagtctttc
    34321 atcaatagtt aacgtatttt tggcagtctg atctaagcca cgttctatca caaactgaca
    34381 aagaaagtct gaaagagctt ccaagttgtc aacgggcagg gtcattcctg ctgcaccagc
    34441 atgtccccca aaggctgtaa ataactcacg cttgccattt aaggcttcaa aaatattaat
    34501 agcttctaaa cttctggccg agccttttgc aaagccatta tcaatggtca aaacgacgac
    34561 cgtttgtcca atagtttcca tgatacgacc agcgacaatc ccaagaacac caggatgcca
    34621 gccagcttgt gccaagactt gaacaggttt ttgaggatca accataacca tcgcttgatc
    34681 aaaaatgtct tggactaagg ccttacgctc ttcatttttt ttcttaatca tcaaggcaat
    34741 cgcttgagct tcttggtcat caaagcccgt cagtagctca atggctggat tagggtcatc
    34801 taagcgccct aaagcattta attgcggagc aatctgaaag ccaatggcgt cttcattaaa
    34861 atgttccaaa tcaataggag acaaggacat caactcctgc aagcccactc tttcggtgtg
    34921 tttgagcatg gccagaccat ttttgactaa aacacggttt tcacctgtca agcttaccat
    34981 atcggcaatg gttccaatag ctaccaagtc tagacagtct gttggtaaac tttctagcaa
    35041 agctgttgcc aatttaaagg caacgccaca gccagctaag tgtttaaagg gataatcagc
    35101 atcaggatgc tcaggatgaa tgatggcaaa ggcttctggc aattcttcag gcaggctatg
    35161 atggtcagtt acaataacat caacttcctg ttcttgagca tatctaatcg cttcatgtcc
    35221 ggccacgcca ttatcaaccg tgataattag ggacacagct tcctgctcaa taaaatattt
    35281 atagacgctc tggttggggc catagccatc tgtaaagcgg ttgggaagat aaaccaaagg
    35341 ctctgctccc atcatgtcta gggtttcttt gacaatagaa gccgacgtca tgccatcagc
    35401 atcatagtcg ccgtaaatga gaattcgttc gccctcttca atagcttgac gaattcttgg
    35461 cacagctttt gccatgtcat gtagcaagta aggatcatgc agttgagaca agtccgctgt
    35521 taaaaactca tctaaggctt cttcagtcct tatccctcga tcataaatca actgagcggc
    35581 ggtctgggtt agaccttttg tctttgctag cttaaagaag ccatcatctg gcttcttgtc
    35641 ttttattttc cagctatatt tggatttaat catttataat taaatacctt tcttgcaaga
    35701 taatcagata atttagggaa aagggtgtaa aattgatggg tcaccgctaa actaaagggc
    35761 aaattcaatt ctcgtttatt tttcccgata atagaaacca aacgcttagc cacttgattt
    35821 ggttggagag taaatttacc aacgctttcc aaataatgtc cagacgggtc agcttggtca
    35881 aaaaacttgg tggcaatggg acctggattc acggtggtca cgtaaacccc tttatccgct
    35941 aattctaagc gaagggcatt ggaaaatccg ataagggcaa acttggtggc tgaataaatg
    36001 ctcgatttgg ctgacgcaat caagcctgcc atggacacaa tattaataag gtgaccttgc
    36061 ccctgctctg ccattttctg accaatcaag caagcaaagt gaatgctcgc tagggtgtta
    36121 acctgaaaca tatcagctat ttcttgggca gaaaactctt caaagccttt gaaagctccg
    36181 tagccagcat tattaatcaa gacatcaata cggccatagc gctggtaaat ctgggcgacc
    36241 attttctcaa tggctactgg attggtaata tccaactcga ggcattcttt gttgtcaata
    36301 tgctgataac agtgttctag gcgttctttg ttacgtccta ataaaatcaa gctgtcttcc
    36361 ttgggtaact gcttaacaat tgcctgagcc agtcctccag aagctcccgt gataacaatg
    36421 attctttgtg ccattaaata atcacttcct ctaaatcttg taccatttta acattttcaa
    36481 atatagtggc ggcatctttt tccatctggc gacaatcacg ccctaaaaag cgagctgata
    36541 catgattgag caagaggcgt tttgcgcctg cttcatgagc gatttgtgct gcctgcatgt
    36601 tggtagaatg cccgtgattt ctcgcaatcc gctcgtcacc cttaccatag gtggattcat
    36661 gaaccaaaac atctgcatct ttagcgagtt taacactggc agacgttttg cgcgtatcgc
    36721 cgataatggt gatgattttg ccttttttag gggcagaaat ataatctttg gcacaaatca
    36781 ggcggccgtc ttccaactca acgtcttgac catttttaat tttgccaaag agtggtccaa
    36841 aaggaacacc cgctgctttt aaggcttctg cgtctagggt tccttctaag tctttttgaa
    36901 ctactcgata acccatgcaa aaaatggtat gcgccagacg ctctgcatag acctcaaact
    36961 tatctgtttc cattatttta cctaatgatt tgtcatcaaa ttcatgaaaa tgaatctgat
    37021 aaggtacacg cgctccagaa acttttagac tagttaacac gtaagtttta atgccaattg
    37081 gtccataaat atcaagatct gtctgttctt cactagcttg aaaagaccga cttgataaaa
    37141 atcctggtaa tccaaaaatg tggtctccat gcaaatgagt aatgaaaatc tttcttattt
    37201 ttcgtggctt aatcgtcgtt tctaaaattt gtctttgcgt tccttcaccg caatcaaaca
    37261 tccagacttc attgatttcg tctagtaatt tcaaggcaag acttgacaca ttgcgctgct
    37321 tggctggttg accagctcca gttcctaaaa attgtaattc cattcaatct attctttcta
    37381 ttgggtgatg taaatcataa attctcgttg ggcaaaatca taatactttt gaccagaaaa
    37441 tgtttggcta agagcccata actcctctac attaaacccc ttaagcatgg tgataccgtt
    37501 tgccagacgg taaatcttcg acaagagtgt gtcatcaggt gtaatgtctt cacattctcc
    37561 ttgctcaaac tgcttagcct gcattttaag ctggcctttg tcctcataaa ccatatattg
    37621 tgggaaaatt tgtgttaatt caaagaagag ttcgggtttc ttaggctctg ctttttcaga
    37681 aaataccaat gcaacttctt ggtcattttg gtaagcaaaa ccataagctt ttccagacaa
    37741 attgaggcgt acaaagggct tttcttcctc aaaagatggt tggtcagtaa aaagccccaa
    37801 agccttagaa agattaaaat aataatcagt tgcagcttgc ggtgactgtt tctggtagat
    37861 ttcagcaaag taggcattaa taacacttgg tggtggtgtg ataaaagcta gtttttgctg
    37921 gtctgtcaaa ctggctaaca gatggtttag gtagttggta tccagactag taaatccacc
    37981 ataggttttt gctaaatcaa tataattatt cataaaaatc gtcaagtctc cattgctgtt
    38041 ttggggaaat gtagcccgaa atatcctcta tttcttggtc aaacgtgtag tggtctaaaa
    38101 gagcaactcg attcaaatca tatagcttgt acgccttgtc ttgatgcact ttgattctaa
    38161 aaggaacaaa ctggtctcga atttggtcaa tgattaaacg tctcaagaga attttgctat
    38221 cctttgaacg ggctgaaatc ctgatatttg gaaaagcagt agctgtgaat tgttctgcta
    38281 tatccacctt gttataaata gctaaacgcg gaatattaag catgtctagc tcttttagaa
    38341 ggtttaaaac cactttttct tgctcactat gattgggatc actcgcatca atcacatgta
    38401 acagcaaatc cacatatttg ctttcttcta gggttgattt aaaagcagcc actaattccg
    38461 ttggcaagtc ttgaataaag cctacggtgt ctgttaaggt tgcttgaaat tgattttcta
    38521 aatagagttg ctttgtagtt gcatctaagg tagcaaataa ttcattggct tcatagtggc
    38581 tgtcatctgt caacaagttc ataatggttg acttgccagc attggtgtag ccaatcaagc
    38641 caattttaaa ggtatctgaa cccacacgac gatctctaat tgtttgacga tttttctcta
    38701 cctgagttaa ttggcgttca atatcagcaa tctgatgacg aatcgaacgg cgatttaact
    38761 ctagttggct ttcacctggt ccacggctcc caataccacc tgcttgacga cttaacatga
    38821 tgccctgtcc aaccaaacga ggtaacatat atttcaattg ggcaaggtgt acttgtaatt
    38881 tgccctcatg gcttctggca cgcatggcaa aaatatctaa gattaactgc atacggtcaa
    38941 tgacttttac ctctaaaaca gcttctagat tagcattttg cctagcagtc aaccgattgt
    39001 tcacaataac tgcatcaatc tcatcagctt ccacaatcgc cttaatctca tccagtttcc
    39061 ctgagccgat aaaggttttg ctgtcgtaac gttctcgttt ttggctgaaa cttgccataa
    39121 ctttgacacc agctgttttg gcaagattag ctaattctgt catggacatg tcaaaatgtt
    39181 ctgttgtctg aagctcaact cctaaaagaa tcactctttc ttgctgacgc tttgtctcaa
    39241 tcatcacctt taccaatcct ccaaattccg taaattctat tataacatat cttcaacaga
    39301 ttctgctaat tttgcgtcat gacttctctt tctgaccaag aaaatccctg accctatcat
    39361 gtactacctg tgggtaatca ggagccgtta tggcagtgaa gctaacagcc attcgattgc
    39421 ggaaccaagt tagctgtcgc ttggcaaaac gtctcgtgtt ttgttttaat tgatcactag
    39481 cttctgccaa agtcatttca cctacaaaat aggggaataa ttccttataa cctatgccac
    39541 gactagcctg aacagtcggg taatgctcat ataaccattt tgcttcttcc aataagccgt
    39601 tttcaatcat acgattcacg cgctgattga tacggtcata gatgacttgg cggtcatcat
    39661 tcaagccgat aataagcggc tcataagccg tctcggcatt ctctaattca tcagcaaact
    39721 gagccaattc aagggctcgg atcgcccgtc tgcgattcac ctgctcaatg gtaatgttat
    39781 tgacttgcaa gcgttcatac aagtcgtgat cgtccaattg ctccaactca ttccgatagg
    39841 cttttaccgc ctcttgatca acttgacctc ctagatgata accttcgagt aaactttgta
    39901 agtaaagacc agttccacct acaataattg gtaatttccc acgactaaca atatctgaaa
    39961 tagatttttg agcatcttga acaaaatcgt aggccgaata agactccgtc acctcacgaa
    40021 tatcaattaa gtgatgaacc gctgcttctt gctcttcttg agtggccttg gctgttccaa
    40081 tatccaactg tcgataaact tgctggctat ctcctgaaat aatttctcca ttaaaagcct
    40141 tggctaaact aatcccaagt gctgtttttc caaccgctgt cggaccaaca atcacaacta
    40201 tttttatttt tgtcattttc ttgtctttct attagaaaag cgtgtccaaa aacgcctttt
    40261 acttgaaaat tagcttattt ttcgctacca tatattataa cataagatta aggaccactg
    40321 aggtccttca ctagaaatgg agaataatca tggttaagaa atatcaattt gttaaaggtc
    40381 ttgctactgg cgtcctagca actgctgcta ctgttgctgg tgctgtcttt gctgtaaaaa
    40441 aaacaatcat tgaccctgaa gaagaaaaag ctgctttcat tgaagaaaac cgcaaaaaag
    40501 ctgcgcgtcg tcgtgtagct cgctaacgtg acaatggatc aatttaaaag agactagcac
    40561 attaaagtgc cggcctcttt tttagtaatc tcgataacta gttagtagta actgtctctt
    40621 gatgagccaa gatgttagct gctttactta ttttgaaata gaaatctcag gtaagcccca
    40681 taaacataag tgactaaagc tgtcgccaac aatacctctc ctagcaaaag aacctgccaa
    40741 cctaaacctt gccaacctaa aaaagataag gtcattttta aggaagtggc actaatcaca
    40801 aacggaaagg taaaagctga aaaacctggg ttaaagggac gattgagtaa gcgaggaagc
    40861 tgtacaacca caaaagcata taacagttgt gagctgacga gaagaaagat aaccattcct
    40921 acttgtggtc taggaaaggt tgctaaataa gacgctgaaa gtaaggacag aggggcacaa
    40981 aacgttgaaa tattgggcat tactgcttga cctagaccaa tcccataagt ctttttggcc
    41041 ataaaaggta aaatcacagc agttaagaga agacaaaccc aaaaaatcac ctgtcctaat
    41101 aaaaactgcc tactagcagg tgctgttaat gccgccattg caattccaac aaaaagcacg
    41161 gaccaggaag gaaaaacgtt atcccatgaa aaagaaaaga caaaacgata ctggtaataa
    41221 gcaatcagga caagatttcc caaaaagaat agccaccaag tgagaaagcc taaccaacac
    41281 cctcctgtca ctttgagaaa gagtgaagac aacaacatcc ctgtcataaa aaaagttgga
    41341 aaaacagagg caattagagg ttgtcgtaac tgcattttgg tatcgttaag gttcctaacc
    41401 atgccaacaa gcaagatacc atagataaaa agagctgcca gaattcccaa ataattaaaa
    41461 atcgaaacat aagtagccaa caaattgcca aatgacaacg ttcctaatgc caatcctgac
    41521 atgaccaatg gtggggtttt aaggtgtttc ataagtgtcc ttttctgcct attaacagct
    41581 atttttagtg acataatctt gtttatttta tcacatttcg agcatttttg acaccacccg
    41641 cgaaagaaaa tttaaggctc ttgccattgt ttttttacat tctcgcaaaa ctaggaaact
    41701 gcttaaaaag ctaaggagtg accactcttc tctagaaaaa tgaaagagta gagaagactt
    41761 ggtctgatct ttacttcttc tttttgattt agtgagctgc attaagcact tgttgaacag
    41821 ctgaatcttc caccattttt gccataaaag ctgccagatg cggataattc tcgtaagatt
    41881 ttggtgtttt ttgagaccat agcgccatgg cgtaagcata agcatctgca atggtttgtt
    41941 gatctcgata aacatgccca ctctccccaa taagactatc taaatgcgtc atagcaatat
    42001 ccatccgctt gtaagaagct tgtttgactt tttcaagcgc ttgattgtct tgagaaatag
    42061 tgtagcgatg agggttgatc attggcctaa aaacaagggg aaaatcacct gttaagaagt
    42121 ccatgatttc gttgaaccga ctttctgcca aaagattatc ttggggaatg agctgagatt
    42181 tcagggtttg tttcgctata aagggcaaga taatacctgc ctgtgtgaca atttgaccat
    42241 catcaagctg taaagcttaa accattccca aaggattgat ggtaaggcag tcttcagaat
    42301 ccagttttac cttctctatc tcgtaataca gaaactcaca attcttttga aaaggtgcta
    42361 gacagagttg gtgttgacgt cttcgctgtt gatggctctg catgacaaaa tgagcttggt
    42421 gtccacgttc acttgtatct tgttgcaaag tctttgcaat ttggtctcat catctgtatc
    42481 acaaaaaaca aaccagtttc ctgatttgtt ttgagatatt aggcacctac aaattctggc
    42541 aaggattctc caatagcttc taaacgttct gcccattctt gacgtgataa accattttct
    42601 tgcacatagc gattacgttc atgtgcacga cattcaggtg agcatcctcg aacatacttt
    42661 gtttcgtttt cttctgaagc aaaaatttgt ttgttacaaa acggatttgc acaattgaca
    42721 taacgttcac aaggtgttcc atcaaaataa tctttactaa tgacagttgg attaacatga
    42781 ttaattggca ccgagatacg atcatcaaaa acatacatcg caccatccca aagttcacct
    42841 tgaacttccg gatcttttcc atatgttgca ataccaccgt gtaattgtcc aacatcttta
    42901 aagccttctc gaaccatcca acccgagaat ttctcacagc gaacaccacc cgtacagtac
    42961 acaacaacac gtttttccat aaacttatct ttgttgtcac gtacccattg tggtaactca
    43021 cggaaattgc gaatatcagg acgaattgct ccacggaagt gacctaagtc atactcataa
    43081 tcattacgcg tatctaaaac aacagtatct tcatcaagca aggcttcttt aaattgttta
    43141 ggatttaaat actcacctgt tgtttccaaa ggattaatat cactatcaaa gttattgtct
    43201 tctaacccaa ggtgcacgat ctctttctta taacgaacaa acattttacg gaaagcttgt
    43261 tgattttcct cgtcaatctt gaaccaaaga tcagcaaaac gctcatcgct gtggacccaa
    43321 tccatatact tctgagttgt ttcataatct ccagaaacag ttccattaat cccctcatcc
    43381 gcaatcaaaa tacgaccttt aagcccaata gatttacaaa attctagatg tttcgcagcg
    43441 tactcttgcg cattttcaat tgaaacatat ttataataga gtaaaactct gattttttct
    43501 gacatttttt ctccttcatt gttattcagt tacttcctat tatatctagg aaaaacaagt
    43561 ctaggcaagt catttgtttg tctttttttc acaaaataga tctcaattta tttttgatga
    43621 gatgagatgc aacccattac tcctttaagc aaatcattag aagctttatt tagaaaatgc
    43681 taagataaaa caaggaggcc aaaatgactt atgaactttg tttagaatat gggacttatc
    43741 cgttgtcacg tgttgatgct tactggggag aagatcaaaa tcccccaaca ttcattcaag
    43801 aagatcgact gctttgccat aaactagaaa ctatgaatca cctgttccat gatttatttg
    43861 taactattga atcacaattc cattatgttg gttttaacat gcctgaaaaa agagcgcaga
    43921 ttcggatact atatcaagaa gtagctacta tattaaaatc caaatataag gattatccaa
    43981 ttaagattga gacgtttctt ctttaaaaat ctaatactaa aaaactaact gtgcttagac
    44041 agatgacaca gctagttttt taggtaaaac tcttcttaaa ttttaaaacg tttcgaaaaa
    44101 tcactaaatg ctttaatagc acttgctaaa aatggtttca ccatctcatc attaattaac
    44161 cttgattact tacaatctta tgaatctgag tcaaataaaa ttcaggctgt ggcattacag
    44221 agacatttaa aacctatact catcgttttc aaggtcagaa agagaatgat tttgaaaagg
    44281 ttcgtcaact cttctagata attttggaca aattttgatc aagaaacaaa aagagcctat
    44341 caaataaggc ttttttatat gatttctatc caccttgagg gaatcgaacc cccatctcaa
    44401 gaaccggaat cttacgtgat atccattaca ctaaaggtgg tcacaacttt gtcaataccc
    44461 ttatagtata tcataaaatg aaataatatg aaataacttt tctaaccaga agacaataaa
    44521 aagttgagga aaacctcaac tttttagatt tattacaatt caatatcacc gaaaagatca
    44581 gccattgaga atccagtttg tgtttctggg agttcgtagt cacgtcttga ttcacgtttt
    44641 ggacgacgtg ggcgtgattg acgtttttct tctttgttgt ctccttcagc ttgcgctggg
    44701 cgttcttcaa gagctttgat tgataatgat acgcgctcat cagctgcatt cacttcaaga
    44761 actttaactg ttacttcttg tcctacagaa agtacatctt ttggattttc aacacgtttg
    44821 tgtgaaattt gtgaaatatg aacaagtcca tcaatacctg gtaatacttc aacaaaagca
    44881 ccgaagtcag tcaagcgttt tactttacct tcaacaacat caccttgagc aagtttttgt
    44941 tcaacgccgt cccatggtcc aggtgttgtt gctttaagtg aaagtgacac acgaccagct
    45001 tcttcgtcaa ttgaaagaac tttaacttca acttcttcac caactgaaac aactgattta
    45061 ggtgacacgt tacgttcgtg agacaattca gtcacgtgaa caagtccgtc aacaccacca
    45121 aggtcaatga aagcaccaaa acttgttaaa cgcgctacag tacctgtaac gactgcacct
    45181 tctgaaatct tagagaacac ttcagcacga gcttctttag cggcctcttc gataacttca
    45241 cgacgtgata ggatgaaacg gttttctgct gcatcaactt ctttgatttt agcatcaaac
    45301 tcttgtccaa caaatttttc agtgttgcga acaaaacgag tgtcaatcat tgaagcaggg
    45361 ataaatccac gaagtccttc aaattcaact gaaaggccac ctttaacagc acgggtacct
    45421 ttaacagtaa caacttcgcc ttcacgacca acaagtttgt cccaggcttt acgagcttcc
    45481 aaacgttttt tagatactag gaaagtaact gtatcagtat ctttacctac tacttgacga
    45541 agaacaagta cttcaactgt gtcgccagct ttaacaaagt cgttaatatc agcatcgcgg
    45601 tcgttagtca attcacgaag tgtcaagaca ccttcaacac ctgttccttc gataacaacg
    45661 tttgcttgac cgttatcaac agttaaaact tccgcagtga caacatcacc agggttcact
    45721 tcgctaacac tgtttagcaa atcttcaaat tcattcattc taaaaaatcc tccaacaaaa
    45781 gctagccaat gctagctttt gataacaata tattttctca aggtaacccg caacgacaac
    45841 aactcttatc tttgacggtc tcagggagca cctgatacct ctgactgggg tagctggatt
    45901 cgaaccaacg catgagggag tcaaagtccc ttgccttacc gcttggctat accccaataa
    45961 aattttttta cagaaaacta attttctaat tagattagtt tccgaaatgg aaagagaggg
    46021 attcgaaccc ccgaacccga aggagcggat ttacagtccg ccgcgtttag cctcttcgct
    46081 atctttccgt aacaacaaaa actattctat catagttttt gtaagctggc aagtgttttt
    46141 gtctaatttt aatggttcaa attgtagttt tcaatgatat tttcaaccgc ttgttcgagc
    46201 gttttgaaga agcaatctac tccatcattt tttaagacag caaatttctc atcaagttct
    46261 gcaatttctc caaccacacg tttaccaatt tgtaattgat agccatcaat cttagtctca
    46321 ttaaccataa ctttgtgatc aatcagttgg atttcaattt ttttatcttt tttactcatc
    46381 actttacctc actttgtcta ttttacacaa aatcaaaaaa gcttgcaaga gcaagctcta
    46441 ttttaatcta cttttacaat ccaaccttct ggtgcttcaa tatccccaaa ttgaatgccg
    46501 accaactcat cgtataaacg gcgtgtcaca ggaccaactt ctgtttcact gtaaaagaca
    46561 tgtaagttat ccttgtactg gataccgcca attggagaga taacagctgc tgttccacaa
    46621 gctcctgctt ccacaaattt atccaattca ttaattggaa catcaccctc aattactgtc
    46681 ataccaagtc tttcttcagc tagttgtaag agtgaatact tcgtaataga tggcaagatg
    46741 gatggactaa gtggcgtaac aaattcatta tttgcagtaa tgccaaaaaa gtttgcagct
    46801 ccgacttctt caattttagt atgggttgct ggatctaagt aaatcacatc agaaaagcct
    46861 gctgatttag cggccttacc tggtagcaaa cttcctgcat agtttcctcc caccttagca
    46921 gcacctgttc cgtaaggagc ggcgcggtca aacgcttcag aaacaataaa attagttgga
    46981 gcaagtccac ctttgaaata gtttcctact ggcatggcaa aaatggtgaa aatatattct
    47041 tcggctggtt taaccccaat aatatctcca actccaatta ataacggtct caggtaaagc
    47101 gttgcacctg ttccgtaagg tggtacaaag tcttcattgg ctttaaccac ttgcttagct
    47161 gcatcaataa attgctctgt tgatacttgt ggcatcaaca agcggtctgc tgtggcttgt
    47221 aaacgcactg cattacggtc tggtctaaat agttgaatag agccatcttt ggtgcgataa
    47281 gcctttaagc cttcaaaagc ttgttgtcca taatgaagcg ctggagcgct ttctgagata
    47341 tgtaaggtgg catcttctgt taattgacct ttatcccatt gaccattttt gtaataagaa
    47401 atgtaacgaa atggcaactt atgatattca aatcctaggt tgtcccaatc aattgctatt
    47461 gtcatcatcg tacctctttt ctgctttttt ctattgtaac tttttttcaa taattttcaa
    47521 gtttattcta ttcttttgta ttaatattct aaatttatca taaaattaaa aagagttttc
    47581 actcttttta acgtgttcta gctaccagta cttcttgatc cgaaatagtg tctgaaataa
    47641 aggagccgtt gctggttctt tccgataacg aaagcgtttc taaagcaatc tcgtaggttt
    47701 gaccagtctt actaatgact tcaagcattt gttgattagt ttcttcttca ggtatatcag
    47761 tgaacaaatc aaactgctca gctgaagtat ctgattgaac gcctccggcc agaaaaactc
    47821 tatgaggttt ggtttttaac tctcgtagga ccaatagtcc acgattggca cgacttgtct
    47881 gaggaatatc atcgacagcc atccgtttga gagaacccct ctgagttaag acaaagaaag
    47941 agttacttgt cacagcaaag gctgaggcaa ggctgtcatc gtttttcaag ttaatgcctt
    48001 taacaccagc agatttcaac ccttgaatag gcacttcctg actggagaat cgcaaagcat
    48061 aaccattttt agtcactaat aggaggtctt ccataataac tggtgacaag gtaaccacgc
    48121 ggtctttgtc atcctttagc ttcacatatt tggttgattt agatttataa gttcgccaag
    48181 gacttagttc tttacgatca aagcgtttga taaaaccatt ttgtgtgaca gccacataca
    48241 agccctggtc aaaggaagtc acaatatcag cgtagagaat ctcttcttct gtcgcgaagt
    48301 ttgaaatggt ttgtgataaa tgttcaccga tatccttcca acgtaaatcg gtcaattcat
    48361 ggattggccg gtaaatgaca ttgcctaaag ttgtaaataa caaaaggtgt tctgttgttt
    48421 tggcctgttt aacgaagatg agttcgtcat catctctctt gccaacttct tccaaactag
    48481 acgcattgaa ggatcgtggg ctggttcttt tgaggtatcc gccccgtgtc acactaacaa
    48541 aagtttcttc ctctgcaatc aagctggcag tgtcaatctc aatgatttga ctttctgctt
    48601 gtaactctga taaacgagga ttggcgaatt tctttttcac ctctcttaat tcacgtttca
    48661 tgacgttata catggttgct tcgtcaccga taatagcgga aagggtcgtg attaagtctc
    48721 ttaaatcatt ttcttcattt tggagagtaa caatatcggt attagttaaa cgataaagct
    48781 gcaaggttac aatcgcttct gcttgttcct cagagaaatc atagctgact ttcagatttt
    48841 ctttagcatc agccttattg tcagacgatc taatcaaagc aataatctca tctaagatgg
    48901 acaagacacg aatcaatcct tcaacaatgt ggaggcgttt ttcagctttg gctttatcga
    48961 atttagaccg ctcaataata atatctttac ggtgactgat atagcttgat agtatttttt
    49021 gaagtcctac ttgacgtggt gtgaagtggt caatagcaac catgttaaaa ttataattaa
    49081 cttgaaggtc tgtgtatttc aacaaataat ttagaatggt ttggctatca gcctcttttt
    49141 tcaactcaat agctatgcga agacctgtgc gatcagattc atcgcgtact tctacgatgc
    49201 ctgggacttt attgttaaca cgcacatcgt caatcttttt gaccaagact gctttgttaa
    49261 cttcataagg aatctcagtg acaataattt gttgtttacc acctttaagt tcctcgattt
    49321 ctgtcctaga gcgaacaacg acacgtccct tacctgtttc gtaggctttt ttgatttcgt
    49381 cagctccttg gataatgcct cctgtcggga aatctggtcc tggtaaaaac tccattaatt
    49441 tctctaaact agctttggga tgatcaatca tatacacaac agcatcaatc acttctgaca
    49501 aattgtgcgg tggaatatct gtcgcatagc ctgcggaaat accagaagac ccattgacaa
    49561 gtaagttagg aaaagctgct ggtaaaacgg tgggttcttt ttctgtgtca tcaaagttcc
    49621 aagcaaaaga gaccgtattt ttctcaatgt cttgaagaag ataacccgca atttcagaca
    49681 agcgagcttc tgtataacgc atagccgcag gcggatcacc atccattgaa ccattatttc
    49741 cgtgcatttc aacaaggatt tcacggttct tccaatcttg actcatccgt accatggcat
    49801 cataaataga ggaatcccca tgtgggtgga aattccccat aatattacca actgatttgg
    49861 ctgatttacg gtaacctttt tcaaaagtat tgccatcctt attcatcgaa taaagaatcc
    49921 gacgctgaac gggtttcaat ccatctcgaa tatcaggaag tgcccgttcc tgaataatat
    49981 acttggaata acggccgaag cgttctccca taatatcctc aagggacatg ttttgaatgt
    50041 ttgacatatt caaatagctt tctttttcat tatttattaa ttacctaaaa aacaatcgtc
    50101 tttagcactt gtaattatct gtgagaaact tgtatgacta aaacaccgta ttttcttcta
    50161 aggtaaactt gacgttatcc tcgatccact gacggcgtgg ggctgctttg tcacccatca
    50221 acacagaaac acgtcgctct gctcttgcca agtcgtcaat agtaacacga ataagggttc
    50281 tggtttcagg gtccatagtg gtttcccata attggttagc attcatttca cccagtcctt
    50341 tatagcgttg aagaatagcc cccttgccaa attctctgcg cagatcttcc aattcaccat
    50401 cagtccaagc ataagcaatt ttttcagttt tgcctttacc ttttgacatt ttgtaaagcg
    50461 ggggaagcgc aatataaacg tgacctgctt ccaccaacgg tcgcatataa cggtaaaaga
    50521 aagtcagcaa aagagtctga atatgagccc catcggtatc agcatcggtc atgataataa
    50581 tcttatcata gttgatatcc tcgaggttaa agtcagctcc cactccagct ccaatggtat
    50641 aaaccatagt gttaatttct tcatttttca agatgtcagc catcttagct ttttcggtat
    50701 ttaaaacttt gccacgtaat ggtaaaatag cctgaaattt gcggtctcgt ccttgtttgg
    50761 ctgaacctcc cgcagaatct ccttcgacca gatacaattc atttttcttg gcatttttgg
    50821 actgagctgg ggttagtttt cccgacaaca agcctttgtc ttttttgttt ttcttaccat
    50881 tacgtgagtc atcacgcgct tttcgagcag cttcgcgagc atctctagct ttaatggctt
    50941 tgcgaactag atgagaagct acttctccat tttcgagtaa aaagaaggtt aatttttcag
    51001 aaacaatgct ctctacaata ggtctagcta aaggacttcc caatttgtct ttggtctgtc
    51061 cttcaaattg caagtgctgc tcaggaacta agatagataa tactgctgag agtccttctc
    51121 gataatctga gccctctaga tttttatcct tttcttttaa gagattggtt ttacgagcat
    51181 agtcattcat cgctttggta atagctgact ttagtcccgt ttcatggctt ccaccatctt
    51241 tggtgcgcac attgtttaca aaggataaga tattgtcgga gaaaccatcg ttatactgca
    51301 aagcaacctc gacttgaaag tcttggtctt gaccttccat gtaaataaca ggtgtcaagg
    51361 tttccttatc ttcattgagg tattcgacga aatcttgaac cccattttca taatgaaact
    51421 cttcaataat cgggtcatct ccacgaagat ctgtcaagct catcttgaca tttttcaaaa
    51481 gaaaagcaga ttctttcaat cgttctgaaa tggtattgaa cttaaagtca atagttgaaa
    51541 aaatcttatc atcaggcata aaggttacaa ccgttcctga tttagactta ggagctgtgc
    51601 caactttttt aagggttgta acaggtttcc caccattttc aaagcgctga cgatacactg
    51661 acccgtctct tgtgatttcg acttctaacc aggcagacaa agcattgaca acagaggagc
    51721 caacaccgtg caaaccacct gacgttttgt agccaccctg accaaattta ccacctgcat
    51781 gaagaatcgt aaaaataact tgaacggtgg gaatccccat ggcatgctgg cctgttggca
    51841 ttccacgtcc gctatctgcc acactgaccg agccatcttt attgataaca accttaatat
    51901 catctccaaa accagacaag gcctcatcga cagcattatc aacaatttcc caaatcaggt
    51961 ggtgcaatcc cgtagcatct gttgaaccga tatacatccc tggacgcttg cgaacagcat
    52021 ctaacccttc taggacttgg atagcatcgt cattataatt attaattgta atttctttct
    52081 tagtcaacac cgacctccag acagtttcat cttttttatc ttacaagttt tttaccaatt
    52141 ttgcaaaata atttgctaat tttttccgaa ttcttttcca gatctagttc tccaatctat
    52201 gaatatgtta taataaggat tatgaaatta ctacttttta ttaccattgc ctatttactt
    52261 ggttctattc caactggact atggattgga cagtactttt accacatcaa cttacgagag
    52321 catggatcag gaaatactgg aaccacaaat acttttcgga ttttaggtgt caaggcagga
    52381 acagctacct tagctattga tatgtttaaa gggacacttt caatattgtt accaattatt
    52441 tttggtatga cttcaatttc atccattgct atcggctttt tcgcagtttt agggcatact
    52501 tttcctattt ttgccaactt taaaggtggt aaggccgtag caacaagtgc tggtgtattg
    52561 ctaggctttg ctccgttata tctctttttt ttagcatcta tctttgtttt agttctctat
    52621 ttatttagta tgatatcttt agctagtgtg gtttcagcta tcgttggtgt gttatctgtt
    52681 ttaacatttc ctgccattca ttttctttta ccaaattatg actacttttt aacttttatt
    52741 gtgatattac ttgcgttcat tattattata agacacaaag ataacattag tcgtatcaaa
    52801 catcatactg aaaatctaat accttggggg ctaaatttaa gcaaacaagt gcctaaaaaa
    52861 taaatttact aaaagcctga ctttctctga aagtcaagct tttttgtttt aactcatttg
    52921 ttaattaggg taaacaacct ctccgtctgc aatagtgtat ttcacgacac ctttgagttt
    52981 gttaccaata aatggggaat tactagcttt agaagcaaaa ttctcagtga tcagacgttc
    53041 ttgcttgtct gcaaaaataa ccaaatctgc tggaccattt tctgccaaat aacccgcatc
    53101 aaaaccataa agcaaagctg gattaagcgt cattttttct aatagtgaca taagtgtaag
    53161 atgacctggt tcaaccaaat gcgtcaaacc taatgacaaa gaagtttcaa gtcctgtcat
    53221 accagatggg gctttagtca tatcatccac agctttttca tccttgtgat gaggagcatg
    53281 atcagtggca atgactgtga taacacccga tttcaagcct tctatgacag ctaagcgatc
    53341 tctttgtgta cgaaggggag gattcatctt ggcgctggta cctgcgatca ataagagatc
    53401 ttcagttgtt gaaaaatgtt gtggggacac ttcagcagtt accttagcac ccaactgctg
    53461 agcaaaagcc actacttgaa ccgattctgc tttagataaa tgttggatat gaacatgagc
    53521 ttgcctgtca taagcaatca tgacatctct tgcaatcata ctatattctg ctacaccagt
    53581 tgccccacaa aaatgaaaat gctcctcagc aataccttca ttaaaaccaa gaaccccatt
    53641 taactgagga tcttcctcat gcaaagaaat gaaagtttgg ttggcattag ccaaatcaaa
    53701 ggcttctttt aaaactttag aactttctaa gggaatacca tcatctgaaa agcttactgc
    53761 tcctgcttcc aacaaagctt taaaatcagt cacgtctttt ccattaaagg cttgggtgac
    53821 actggcattg gtgtaaatat gaatcttttc tttagctgcg ctagctagca cttcctgtaa
    53881 agtttcgaca tctgaaatga caggattggt attagccatc atgacaaccg tagtcacacc
    53941 acctgctgct gctgccaagg ctcccgtgtg aatatcttct ttgtgcgttt gaccgggttc
    54001 tctaaaatgc acatgaatat cgaccaaacc cggggcaaca ataagaccac tagcatcaat
    54061 cacttgagct tcttggcatt caatagctga agctatcttt acaatctgtt taccatcaat
    54121 caagacatct gccacttgat ctctctgcga tttcgggtcc ataacgcgtc catttttgat
    54181 gagtatcatg ttttctcctt tatttatctg ttttaaccat aagaccttac gttctttaat
    54241 aggaaaaatc tactgcactc atttcactta ttaaaggtat caaaatgtct agattaagct
    54301 atttagttga aatggctggc ttattttagc caatctacgc cagtcatgcc ctctttttct
    54361 aaaatagcat tggttcttga aaagggtttg ctgccaaaaa agccacgata agatgacaaa
    54421 gggcttggat gagggctctc gatgatgtgg tgctttggat tagtaatgaa ggcttttttc
    54481 ttccttgcat aagctcccca caaaatgaaa actacagggc tgtctttctc attcagcact
    54541 ttaataacag catctgtaaa tggctcccat attaagcctg catgcccatt agcctggcct
    54601 gctggcactg ttaagcaagc attcaaaagc agaacccctt gactagccca agtgcttaaa
    54661 tcatgatggt cacgaggacc aatgtcatct gctaattctt ttaaaatatt aataagggat
    54721 ggcggagcag aaatctcttc tggaactgaa aatgataagc cctgggcctg tttggggcca
    54781 tgataagggt cttgtcctaa aatcaacact ttggtttctt ccaaaggagt gacttgtaag
    54841 gctttaaaga cattttctcg tggaggatag acaagaccgg aagcataggc ttcatctaaa
    54901 aagtgattga tgcgaccgta gtaatgctcc ggcaaaaatg atttgatttt ctcatgccaa
    54961 attgaatgag ccataagatt cctttctaat cacgtcagtt tctaagttaa atacgatgtc
    55021 cttttctatc tagtttacca cattttaaat agaaagaccc tgaaaatatg atatttccag
    55081 ggttaatatt cgttttttac aggatattaa acaagtgcta gcttattttc taagaattct
    55141 tggtaacgtt ctaacaaaaa agcaataagc caatagataa aggctactaa aatatacatg
    55201 gacatgtaat cccactctct accgccaata attttggcat tttgaaaaat atctggtacg
    55261 gtaatcatgg cagctagcga cgaactttta accatatcaa taatgacatt tcctaaagga
    55321 ggaactgcaa ttcgaaaggc ttgaggaaga ataattttct taaccataag agcatatggc
    55381 aaccctaaag ccttagctgc ttcccattgt cccttgtcaa tagcttctat agaagagcga
    55441 aaaacttcag aaatataggc agcgctaacc atagaaaaac caagataagc gcaaagtaaa
    55501 gctggcaatt ctaaaccata ataaggcaac ccgaaataaa gaacaaagag caccacgatc
    55561 atcggcacac ctcgcataat agagatgtac gctctaacaa gataatgaat caacggctga
    55621 cgtgaccttc ccagaagggc taggcctaaa cctaaaaaca gacccgtaaa aaaactgagc
    55681 aacgaaatac caagggtata aggtaatccc gataacacaa atcctaaact atctttcatt
    55741 aatgggatat taatcatgtg gttaggactc ctttaacatc aagtattagt taacgtcttt
    55801 cgtatcaatg acgggaattt ttttgtcttt gccaaaaggt tttgtcagat cttgaccagc
    55861 atggtaggtt tcagagattt tcttcaagct accatctttt atcatgtctt taataacagc
    55921 atctattttt ttcttgaggc tatcgtcttt tttgttcata acaattcctt gttcggttgg
    55981 gttgtactga acatcgctca ttttgacatt tagatttgga ttttgagata acatgtaatc
    56041 cacaaataat ttttgagctg ggtaatcatt tgggatgaaa tctgttcgac cgttagccac
    56101 atcgtttaga taaacatctc ctgtaacatt atcgtaggtc accagttcag ctccctgttt
    56161 ttgcgctact ttcatgtatt ctgttccaga agcgccggct gctttttttc ctttccagtc
    56221 gctaagatct tttttagcca tgtttgagct gccatcagca cggacaatca tgcctcctac
    56281 cccgtattta taaggcgtag agatattgta ttttttctga cgttttgggg taatgtcaaa
    56341 gttgttcaaa gaaatatcca ctcgaccact atcaactgag gtaaaggctt ggtcaaatcc
    56401 tgtttcaaca aacttgactt taatgttaag tcgtttaccg atttctttga ccatatctac
    56461 ttcgtaacct actaactcat tattatcgtt gtaaaaagaa gttggctgat aggttcctgg
    56521 ggtagctact tttaacaccc ctttttcttt gatagtgtcc caacttgatt tttgagattg
    56581 atgtggcgca catgctgcca ataaaaggct ggcaagtccg atagacagta atgtaactga
    56641 tttcttcata gtaactcctt tattaagata agaatagtat aacattttga ttaagttgtg
    56701 ttatcagtga gcactcaatc tctttaaaaa gttaatctat aatagttaaa ctgttttata
    56761 agattattat tgaggaatct tcaataatcc tgctgcctca aactgttctg caattcctaa
    56821 taataggtcc tctctacctt tggctgcaat tagttgaatg cccattgaga gaccttcctt
    56881 agtttcataa gtaggtaaac taatagctgg ctggccagtt agattaggca gagctgtgta
    56941 aggattaata gccagactct taccaaacat ggtttcgacc aaattgaatt gctcttcact
    57001 gctgaaaatt tcagcttgag ccaagttagc catgagtttc gaatctggga caagttctcc
    57061 gtgcttagga gctggtgtgt tggttgtgaa cgtcaacaac aggtcataag tctcatgaaa
    57121 actagccatg gttgcactgt acgtatccca tttttgaagg acctgtgagt aacgccaagc
    57181 tgggatgtct ttacctgatt ggtaaatagc ccaggtcatg gtttccatat cgtctttggt
    57241 catcggacgt ccaaaagtgt cttcaatatc agcaaacata gcagccgttt ccacactatt
    57301 cataatataa taatgacgaa tcacttctgt catatttaca ggaaactctt ccaattcaac
    57361 tagctgatgc ccttgttctc ttagccaagt tactgcttgt cttaaagctt ttgcagtatc
    57421 taaagatact gggctaccgt cacttaaccg ttgataaaaa gcaattgtca agggacgttg
    57481 tagcgattga taaatactat cttttgttaa ggtggctagg gggaaggggc tttccatttg
    57541 ttccatctgt aaatagtaga gtagatttct agtatctcta acggatttgg tcaaggcaaa
    57601 atgaactgag gctccttgcc agcttcgata agaaccaggt ccgacaggca tgcgcccacg
    57661 gctaggtttg agcccaatta agccattaaa cgaggctgga attcgaatag aaccaccacc
    57721 atcactagca gttgctagcg cactaattcc tgatgacact agagcagcag ctcccccaga
    57781 tgagccacca gcatttcgtg tattatccct tggtaaattc actggtccgt gcaaactgct
    57841 atcgctaata tttttaaagc caaattcagg cgtgttggag cgccctaaaa taataaagcc
    57901 taaagcttct aatcttttca caaataaatc cgttttagtc gcttgatact ctttaaacaa
    57961 tctggaacct gaggttgatg agtgaccttt taattcttgt cccaaatctt ttaaaaaaag
    58021 aggcacacca gcaaaaggca gacctgaaaa atccctttgt ttggcttctt ctagggctgc
    58081 ttcaaagcgt tctgaggtaa tagcatttaa ggtagggttc aactttttag ccttataaat
    58141 agcctgggtt actaattcta gtggtgtggt ctgaccagtc tgaacagcaa tagccatagc
    58201 cgtcgcatct tgataagtca tagctactct tcctttctaa gatatgaatc ctaaaacatt
    58261 cttttttatt aaaacacaaa taaaagaaaa ccaccattaa aaggttagaa attagaatgt
    58321 gatgtgtaag gaatgtagac aaaacaatca aaacataaca ttttgaattg gggctaaaga
    58381 gattttttca ttaaaaataa acctggctga tagttcctta ttgttgccag tttacctggt
    58441 cttctttaaa ttttttaagt aaatgtaaac catcattagt aatatagcct tgcaactttg
    58501 caactgcaat tagctcagta taattagaaa gcgtaatcaa cttgatacca gcttctttaa
    58561 aattctgact agcttttggt aactcataag tgaaaatcgc aacaactccc agaacgtcag
    58621 ctccctcacg actggctgct gcggctgcat ctagtaccga accacctgtt gaaatcaaat
    58681 cttcaatgat aaccattttt tggcctttaa gcacacgccc ctcaatctgg ttgcctgctc
    58741 catgatcttt tggcttactg cgaatataag caaaaggaag agtcatttta tctgctataa
    58801 tggcaccgtg aggaatacct gctgtagctg taccagcgat gacctcaact tctgggaaat
    58861 gagccttgat agtttccaca aacccattct caattaaatc acgagtctta ggataagaaa
    58921 gggtgacacg attatcagtg taaataggtg atttaatgcc agatgcccac gtaaaagggt
    58981 cttcaggctt taaataaaca gctttaatat caagtaattg agttgcaatt tggcttgcta
    59041 gtgtcatttc ttattctcct ttttcttctt taacagacaa gtgttggttt cctaatctta
    59101 tctgttagcc tgcccactca gcttttatgg cttggtaagc agccactggg tcttttgcct
    59161 gggtaattgg tcgccctacc acaatataat cacttccaat gcgtcttgct tggtttggag
    59221 tcatcactcg tttttgatcg ccaatattac tgcctttagg acgaatgcca ggagttaagc
    59281 aagtaaatcc cgtaggcgtc accgccttga tagcttctac ttcttgtgcc gaacaaaccg
    59341 ccccatctag ctgagcttta gctgccccct tgctgtaatg caaaacagac tctaaaagac
    59401 tcgtttgaat attttgatca ccacgcattt ggtcttcact ggttgaagtt agctgcgtta
    59461 ccgcaattaa ggtagggcct tgacctagtc cttctcgagc tgcttttagc atctctacac
    59521 caccagctgc atgaacagtt gccatatcaa tatctaattc tttaagaact gccatagcag
    59581 cacgaacagt atttggaata tcatgtaatt ttaaatccaa aaatacatta tgccctaacg
    59641 atttgatata ccgaacaata tctggtcctt gcacataata taattccata ccaattttca
    59701 cgtatagttt ttcttctgct ggaaataagt ctaaaaatgc tttagtctcc tcaaatgatg
    59761 aaaagtctaa agcaataatt gggcgttctt ctttcataac ttatcctttc tataatgtgt
    59821 caatatctca ttaagcacca aaaagcctcg ctaccaaaga agcgaggcta cagcaaacta
    59881 aaagggaatt cccttttttt atcttgtata ccttgtttgc ctctctggac tcacttaaag
    59941 gtgtctaaat ttaaactatt atagcttttt taaaacaaga tgtcaaaact ttttgttatt
    60001 tgaacacatc tttcctacta tatctttttt tgtatactta cgtcaactca ttttatcatt
    60061 tttacccaat catttattca aactcacctg tgggtacctt agggctttgg taatatttta
    60121 taaaagcttc ttcttcaaga ttaaccctct ggatatgttc atagagcaca tccggaatag
    60181 gagcttcctt aagtgacaaa gcactcactt ttaaagatga aaaaacatca cttaagaccc
    60241 caaaaactcg aatgcgttta tctccaataa taatttgaaa gacttctttg gactggcata
    60301 aatctatcag atgatccact cctttaatcc aattatcgac aatatccaaa gttttgttca
    60361 tcaacttaaa agctgattct tcataccaaa aggtagactc aatcaagcgt tgctgcatat
    60421 ccaaggtatc aatggccgca acaatatctc ccaactcaga ctgtactaaa tgagctggaa
    60481 attcccctcc aagtatagaa ggagttcgtt ctacaagctt cagttcatct cgaaaaagag
    60541 tcaaatagtg gatactgacc aataactttt ggattaagat ttgatgacta ttttgttcag
    60601 taacattcac acgaccacca ccctttgatt agaaaaatac ctatttgtta atactattaa
    60661 tataacacaa gaaaaataaa agggcaatga aattgataga ttcaactctc aaaattgaat
    60721 catatttatc ctaggccata ttgagaaaag actccagcgc tttttcaaag ttctaaaccc
    60781 atgatgacgt ttgtgcttgc gtctttattt tttttgagct agactgtact tatgagaatt
    60841 caacaactgc attatattat caaaattgtt gagtgcggct ctatgaatga agctgctaag
    60901 caattattta tcacacaacc aagtctatcc aatgcggtca aggatttgga aatggagatg
    60961 ggcattacca ttttcaaccg aaatcctaaa gggatcacct tgacaaaaga tggggtggaa
    61021 tttttatctt atgctcgcca aattatagag caaacatcac tgttagaaga ccgctataaa
    61081 aaccacaata ctggccgaga gctctttagc gtttcatctc aacattatgc cttcgtagtc
    61141 aatgcatttg tctcactcct caagcggacc gacatgactc ggtatgaact ctttttgcgc
    61201 gagacaagaa cttgggaaat cattgatgat gtcaaaaact ttcgatctga aattggtgtt
    61261 ctctttatca acgactataa ccgagatgtc ttgaccaaat tatttgatga caatcatttg
    61321 accgcctctc cattatttaa ggcacagcct catatttttg tcagcaaatc aaatcctcta
    61381 gcaactaaaa gcctattaag catggacgac ttgagagatt ttccttacct gagctatgac
    61441 caagggattc acaactcctt ttatttttca gaagagatga tgtcccagat gccccacaac
    61501 aaatcaattg tggtcagtga ccgtgccacc ctctttaact taatgattgg tttagatggc
    61561 tacaccgttg ccagtggtat tctaaatagc aatctaaatg gtgaccagat tgtggctatc
    61621 ccgctagatg tccctgacga aattgacatt gtttttatca agcatgaaaa agccaacttg
    61681 tccaaaatgg gggaacgttt tatcgaatac cttctcgaag aagtaacctt tgattaatgt
    61741 ctttttcaat cgtcatacta aaaaacacgg tagtaagacc tgtatcggtc aagttatcgt
    61801 gttttttgat aaatatcaga caatagattg tctccaaagg ctcaactcct ttttaataat
    61861 ggcattcatg tcatcaaagt aaaaaggctc acgtcggtag attacaccag aaaaagtcgc
    61921 caaggtgata atgttttcca aaaaagcctg agaagaaaaa tgagtggtgc gatgcagttc
    61981 ctcttcatca aaaggcttaa tcaaatgagc cagaggatta cggacagcct tttcaaaatg
    62041 tcgtaacaaa aaaaccaagt ccttgatttc ctgaggtaag gaggtcaacg tcaacatgtc
    62101 tgctaagctt ttggtcgtca cattgcgaga ctgcttttga gacaggtaag ccttgaaaac
    62161 ctcgtgatca gattccaaca tggcctgaaa atgccaggta tcatattggt cattctttgt
    62221 atcaaaaata taagccttga agtttggaat ttctgacgtg attagtctga gaaaaagtcg
    62281 gtaaagaata ggcgacactg acctgacgaa atcaataata gcaccgtttt ttactttaac
    62341 ttctaaatcc aaaatgtaat tggcaatctg ctcatcttca agcgattggt taagccataa
    62401 tgaacagtga tactggtctt caatggtccg attctctgct gcatgttccg ctagaaaggc
    62461 aaggttcaat tctctgcgct cttttaacat ctctagtaaa tagagggcag ttttagggat
    62521 atttggcaag gctttagcaa ttctaaaggc gtgattataa gcatagacat ccaaaaggat
    62581 gtctagtgtt tgattattag tcattggcaa ttaaggtttc caagccgacc ttcatcatat
    62641 cagtgaaagt tgtttggcgc tcttcagctg tagtatcttc ggttggatca ttcaagttat
    62701 ctgagatcgt catgattcca agggctttga catggtgctg agcagctaaa tagtaaagag
    62761 ctgctgcttc catttcgatg gctttaacac ccaatttacc aagagccata ttacgctcag
    62821 gcatgttagt gtaaaagaca tcagaagaaa gcacattccc aacgtgagtt gttactccca
    62881 tttctctggc gatatggtaa gccttgtcaa gtaaaccaaa atcagcaatt tgtgggaaat
    62941 caaattctgg gaagtcatta cggataatat ttgagttggt tgctgctgct tgggcaagca
    63001 ccaattcacg cacatgaact tctgggtcaa tcgcacccgc agtaccaaca cggatcaatg
    63061 ttttgacacc gtaatcaacg attaattcac gagcatagat agagatcgaa ggcatcccca
    63121 taccagttcc catgacagaa acgcggtgcc ctttgtaagt tcctgtgtag ccaaacatgt
    63181 tacgcacttc gttaaaacac actgcatctt ctaggaaatt ttcagcgata aatttcgcgc
    63241 gcaaaggatc tccaggtaga agaattttat cagcaatatc gccttttttg gcagaaatat
    63301 ggatagacat aaattatgat actgagggcg ttaaaagaac gatggcaaaa taggaagttg
    63361 gcagaaaatc ctgattttct agacaactta tctttttgac caagtcctta gcccgaattc
    63421 aaattaatga actcagccct cacctttctt atttttttat tttttataag gaagaaaaac
    63481 agttctccgc ccgtattcaa ttcaatgaat acagttagcc ctacctttcc ttttattttg
    63541 tagaaggtca gagggcggac agaaagcgac tgaaatttag ggaactaacg ccatgtgtga
    63601 gctcaaaagt tagtttatct agtttccgag ctttccgccc gtattcaatt gaatacaacc
    63661 ccattccttt cataattgat attgaataga agagagctaa aaatcgagtt caaataagaa
    63721 aggactaact ctctttcctt tattatttaa aaattaatac gaagaaatgt tcgagtccat
    63781 actcaattta aaccgaatac gtctctttcc cttcgttggt tatgttagtt agaaaagcta
    63841 gaatctgagt tcaacattca tggaactcac actccgtgat tattttttga tttttttatt
    63901 gcgaacagat gtgccaaact gacgacataa aataacattc ctgtaataat gctccagttg
    63961 attttgttcg tcctacaact ccgctaaaat agctttaaca agacctttga aatcttcttt
    64021 gatatgttgg gtcacttcca caacttcttc atggttcaat tctgattgga aaccagctgc
    64081 aaagtttgta atagctgaaa taccaagcac tttaagtcca gaatgagcag ccacaataac
    64141 ttccggtaca gttgacatgc caacagcatc tgctcctaat actttgaaag cacggatttc
    64201 tgctggtgtt tcataagttg gaccagtcaa gcccatataa acaccatctt ctagtttgat
    64261 gttcattttt tcagccactt catgtgcctt attgcgataa accttagtat aggcatcaga
    64321 catatcagga aagcgtggac caaactcgtc caagttttca ccgattagtg ggttatttcc
    64381 ggtcatattg atatggtctg tgattgccat caaggtacct gggccgtagc cgataccacc
    64441 agctgcattt gtgacaagaa cgccttcaca tcccagagct ttcatgacac gaacagggaa
    64501 agtcaccact tcaagcggat ttccttcgta aaaatggaaa cgtccttgta aggcgagcac
    64561 tttacggcca gctaaatctc cgtaaactaa ctttccagca tggccaacaa ccgttgactt
    64621 gccccagttt ggaatatctg cataatcaat aacaatagca ttttctactt cttcagcaag
    64681 ttcaccaagg cctgaaccca gaattaatcc aaattctgga gtttcaattc ctttagtaac
    64741 taaaaagtct tttgtttcgt taattttagt catcaatgac atctttttct ccttttacct
    64801 caatagggtt cacactctcg ccatcctctt taatataagg actaggcagg ggaacaggaa
    64861 gaatatccag taaggtctca gaaggacggc aaagttttac tcctttgcga gtcacaacaa
    64921 taggacgatt gatcaaaatt ggatccgcca acatggcatt aataatgtct ttctcagcta
    64981 ctgcttgatt tgctaatcca taagcctcaa attcaggaac attggttcgc aagagttctc
    65041 ttgcagagat tcccatcgat tgtaagagct caattaaagt ctcccgatta ggtggcgtct
    65101 gcaaatactc aataatagta ggctcaatac ctgcatggcg aatcatggca agaacattac
    65161 gtgaagtgcc acagtttgga ttatgataaa tcgtgatttc ttccattatc aattactaac
    65221 tttcttattt taagtgacta aggaatgact ccccaatcat ggctgtatca actccaaaat
    65281 tttctgcaac agttgctgaa atatccgcaa aatgcccttg tggaataaga ccatttccag
    65341 taaaggatac agagtaggca agcaatggaa tgtattcacg agtgtggtcg gtgcctgcat
    65401 aggttgggtc attaccatgg tcagcagtga tgagaagcaa atcgtcttct ttcatatttg
    65461 cgatgatttc aggtaggcga ttgtcaaatt catgcaagca gtcgcgataa ccctctgggt
    65521 cacgacgatg accaaagttg gcatcaaaat caacgaggtt agtaaaggag aatcccttag
    65581 taaactcagg taattgaaga gtcttaataa gcgtgtcaat accatggctg ttcgacttat
    65641 tatgtcccat gtcgtttgta atacctgaac cattaaagat gtcattaatc ttaccaacgg
    65701 cataagttgg gacacctgcg tcagccagtt tgttcaaaac ggtatcttgg aaaggagaca
    65761 ctgcatagtc atgacggtta gccgtacgtg taaagtttcc tgggtcgcct acataaggac
    65821 gcgcaataat acggcctaaa agggcagggc gttcaagcgt gatggagcga gcatattcac
    65881 aaatcttgta aagctcttcc acaggaataa tgtcttcatg ggctgcaatt tgaagcactg
    65941 ggtctgctga ggtgtaaaca atcaactcac ctgtttccat ttgacgcgga ccaaaatcat
    66001 caataaccgc agttcctgaa taaggtttgt tggcctcgcg aatgattttg cggcctgaga
    66061 attcttcaat cttagttaaa atctcttctg ggaaaccatt ccagaaagtg tcaaaaggtt
    66121 ccgtaatgtt caagcccata atttcccaat ggcctgtcat ggtatctttt ccaagagata
    66181 cttcttccaa tttggtcacg taaccagtag gattgtcttc tgttggaaca gtttttagtg
    66241 gtattggacg agaaatgttt ccaagtccaa tttttgccat atttggaacc gataagcctg
    66301 ctgcttctga aatatgaccg agggtgtcag aatctgtatc agctacacca gcattaaaga
    66361 atttgtcagc atctggtgct gcaccaatac cgacagagtc taaaacaacc aagtgaatac
    66421 gattaaattt tgacataagt ctccaatcta ggggaggtta cggttgactc cccaatacct
    66481 ataaaatata ttaacgctgt tatatgctaa gactagcctg ctgtaggcgc ttctaaaaca
    66541 gtaacgccgt ctttactagc aacaatcacc ttatcaacca taccattaaa gagaccgtgt
    66601 tccacaacac caactgttcc atctagcagg tgaccaaaag caacaggatc tttaatacaa
    66661 cccaaatcaa gatcaataat atagttttgc atgtctgtca ccaaacgact atcacctttc
    66721 atacgaaaag acggtttgta accagctttt tcaaagacac ggaacaaacg atcagcacca
    66781 tattgaacga cttcgactgg taacttaaag gcacccagat gctctaccat tttagaagca
    66841 tcaacaaccc agatgtattc ttttgttggt gttgcaacaa ttttttccat tagtaaggct
    66901 gcgccgccac ccttgattcc attgaaattc ttatccactt catcagcacc atctacggtg
    66961 aggtcaatgc tatcaatatc atctatcgat tttaaaggaa tgcccaagac ttctgcttgc
    67021 ttgctagtca cacttgacgt agtcacacca accacttgca gtccttcttg ctttacgcga
    67081 cggccaattt cctcaacaaa atagtaagcc gttgacccag ttccaagccc aatggtcatg
    67141 ccatcagtca catattgcgc agcggtaaca ccggcaattt ttttgagtgc ttccatgatt
    67201 cccccttata atagaattgc gtcttagcac tttattatac catgaaagcg ccaacttgtt
    67261 aatgaaaaca tttgtttttc tctcattgac agcctaagtg ctatagaata ggatgatctt
    67321 tttaaaactt atgatttatt ctaaagtcat catataagca cttacttttc gtgtttattt
    67381 agctttttga aaatcaagtg ttttttgttt gacctttact gaccttttga ttataataat
    67441 actataagat gagtaaaaat aatgtctgaa tcattttatt agcaaaggat ttcctatcat
    67501 gtgaaatcct aacaacgatt ctgcgactgc ttacaaacat cttatagtct attttttatt
    67561 gcgaggtaac tgatatgaca catttttctg gtaaagatcc ttttgtaaat atggatgata
    67621 ttttcaatca attaatggca aacatgggtg gttatcgtag cgaaaatccc cgctatctcg
    67681 ttaacggtcg cgaaataact cccgaagaat tccaacacta tcgtcaaaca ggacaactcc
    67741 ctgtagcgac aaccaaagca acaaattctc aaatgttaac acctaaagct gatagtgtat
    67801 tgactcaact cggaactaac ttaacacaag aagctcgtca agggcatctt gaccccgtca
    67861 ttggtcgtaa caaagaaatt caagataccg ctgaaatctt ggcacgacga actaaaaata
    67921 atcctgtcct tgtcggagat gctggtgtcg gtaaaactgc agtcattgaa ggtttagctc
    67981 aagccattgt taatggagat gtcccagctg ctattaaaaa caaggaaatc gtatcaattg
    68041 atatttctag tcttgaagct ggtacacagt atcgtggttc ctttgaagag accattcaaa
    68101 acttgattca agaagtaaaa gaagctggga atatcatctt attttttgat gaaattcatc
    68161 aaatagttgg tgctggtgcc acaagcagtg attcaggctc aaagggactt gcagatattc
    68221 ttaaaccagc cttgtcacga ggagaattaa cactgattgg tgctactact caagacgaat
    68281 accgcaatac tattttaaaa aatgctgctc ttgctcgtcg ctttaatgaa gttaaagtga
    68341 atgcaccatc agctgaagat acttttcaca ttttaatggg tattcgtaat ctttatgaac
    68401 aacaccacca tattaccctt cctgataatg ttctaaaagc tgctgttgat tattctattc
    68461 aatacatccc acagcgtagc ctgccagata aagcaattga cttactggat atgacagctg
    68521 ctcatttagc tgcgcaacac ccggtcactg acttaaaaac actcgaaact gaaatagcta
    68581 aacaaaaaga gagtcaagaa aaagctgtcg ccaaagaaga ctttgaaaaa gctttagcag
    68641 caaaaacacg gattgaaaca ttacaaaagc aaattgagca acataaccaa agccaaaatg
    68701 tcactgctac agtcaatgat atcgcagagt ctgtagagcg cttaactggt attcctgtct
    68761 ctaacatggg gactaatgat ttagaaaggc tcaaaggaat tagcagccgc ttaaaaagcc
    68821 atgtcatcgg acaagatgag gctgtcgcag cagtagcacg tgctattcga cgaaatcggg
    68881 caggttttga tgatggtaaa cgtccaatag gttctttcct ttttgtgggt ccaactggtg
    68941 ttggtaaaac agagttagct aaacaattag cacttgattt atttggttcc aaagatgcta
    69001 ttattcgact tgatatgtcc gaatataacg atcgcactgc tgtgtcaaaa ttgattggga
    69061 caacggctgg ttatgttggc tacgatgata ataataatac cctaactgag cgtgttcgtc
    69121 gtaaccctta tgctattgtc ttactagatg aaattgaaaa agctgatcct caaattatta
    69181 cacttcttct acaagtatta gatgatggac gattgacaga tggacaagga aatactatta
    69241 actttaaaaa taccgttatt attgctacat caaatgccgg ttttggccaa caagatacgg
    69301 aaacttctga gtctaacatt atggatcgca ttgcacctta tttcagacca gagtttttga
    69361 accgttttaa tagtatcatt aaatttaatc atctgcaaaa agaaagttta gaagaaattg
    69421 ttgatttaat gctagctgaa gtaaatcaaa ccaccgcaaa aaaaggtatt agtttaacga
    69481 ttactgatga cgctaaagct catttgatag atttagggta caatcacgct atgggagctc
    69541 gtccattacg acgtattatc gaacaggaaa ttcgtgaccg tataactgat tattatcttg
    69601 accatccaga agtcaaaaaa cttcaggcaa tcctaaaaga aggacaacta gtgattaggc
    69661 aaaacgatca gtaataaact tcttcaattg aataattttc aaaacatgaa aaaatccgtc
    69721 ttgtgactac gttatacact tgacggactt ttttttagcg cttttttaaa aataattttt
    69781 tcatcgccta ataactcaag tttctttcca aatctggtgt aatcaaaata accattaggt
    69841 gtgagtaatc cttttgaaaa agaagtatgt cccgctgcta cttcttcttt gatatcaatg
    69901 gtgtgcaaaa tacgattaac ttttcctttg agaacaacag cagataaatg tcctgttttt
    69961 tgattatatt tgcctccgat ctgagaagct aatttctcaa aagcttgatc taaaatcatt
    70021 tctaattcat ggctagctgg catactattc tcaataattt ggcgttcttt actactcaag
    70081 tcaccaacaa ctaaatgatt ttcctttaat gtctttaata attcattttg gtgataagca
    70141 gctaaacgtt tgacaaaaat gtctcgttca acttgaacac taaaactcaa gtcaacgttg
    70201 ttatttttaa ctgttagtat catctgagat gacgttaata aactatcatc aataagaggg
    70261 ctctgatgca aaccagtaag acgtttagca aaatcatcgc ctaacttctg atcaatgctt
    70321 gttgttcgcc agataccttc aatagagcta ttacgatatc gaacaccaaa aagtacagca
    70381 agcaagataa gaaaaataat aaatacaaaa agggcattgc attgatggca taacaatgac
    70441 tttcctttag agcgcccttg ggctacttgt gtcatgactc atctccttaa tataaaatac
    70501 tagcatgtca tcattaaggc gtcaagtaaa atcggtaaac ctaactatga ttttttatgt
    70561 tttcgataag aaaaacaccc tctactgaga atgttttagc atagataaat caacacctat
    70621 cgctagacct gctctaaaat agcatcccaa gaatcatcga taccaatacg ttctaccgaa
    70681 gaaaatacta taaaagtatc agatttgtca aaattcaaag ctttcttgac cacagactca
    70741 tgtttgttcc atttgccacg aggaatctta tctgctttgg ttgccacaac aataacagga
    70801 atgtcatagt acttcaaaaa gtcatacatc tgaatatctt cttttgaagg ggcatgacgt
    70861 aaatccacca aactcacgac tgcacgcaga ttgtctctgc tggttaagta ttcttcgatc
    70921 atctttcccc atttggcccg ttcactttta gaaaccttgg catagccata accagggaca
    70981 tccacaaatc taagcttgtc atcaatattg aaaaaattta atagttgggt ttttcctggc
    71041 ttgcttgagg ttcttgccaa atttttgcgc cccaatatgg tattaataaa acttgattta
    71101 ccaacatttg aacgtccagc gagggcaatt tctggcaaat catcttgggg gtaatgagat
    71161 ttattggcag cgctcaataa aattgatgcg ttatgggtat ttaagacttg ttcttcagcc
    71221 attatttctc cttaagctgt ctctaaaacg ggttttgact tgccttcgac agcagccttg
    71281 gtgatacgca cttttgtgac atcttcttgg cttggaattt caaacatgat gtcaagcatc
    71341 gtttcttcga taatagaacg aagaccacgt gctcctgtct tacgttcaat ggctttattg
    71401 gcaatggctt ctaaggcttc cttatcaaat gccaattcga cgccatcgta agaaagaaga
    71461 gcctgatatt gcttgaccag ggcatttctt ggttctgtca aaatttgaat caaatctgat
    71521 gtgtttagtt gttcaagggc tgccacaact ggtaagcggc caataaattc tggaataagc
    71581 ccaaactttt gaatatcttc tgaaataatt tcttgcatat aagaagcatt gtcatcaatt
    71641 ttacggctgt tttgaccaaa accaataact ttttcaccta aacgttgttt aacgatctcc
    71701 tcgatgccat caaaagcccc gccaacaata aacaagatat tttttgtatc aatttggatc
    71761 atttcttgat tagggtgctt ccgcccacct tgtgggggaa cacttgctac cgtaccttca
    71821 ataattttaa gaagggcttg ctgtacacct tcaccagata catcacgtgt gatagatacg
    71881 ttctcacctt ttttagcaat cttatctatt tcatcaacat aaataatacc acgctctgct
    71941 cgttcgacat tatagtcagc agcctgaatc agtttgagta aaatattttc cacatcttct
    72001 ccaacatacc ctgcttcagt caaagaagtt gcatctgcaa tagcaaatgg tacattgagg
    72061 cttttagcca gagtttgtgc taagaaggtt tttcctgaac ctgttggtcc aatcatcaaa
    72121 atgttggact tttgtaaatc cacatcatcg tcatcacgac tttctgtaaa cgagacacgc
    72181 ttataatgat tgtacacagc aactgataaa gcacgtttag cacgatcttg accaaccaca
    72241 tattgattta aaacatcaag gagctctttt ggttttggaa cttctgtcaa atcggcaagg
    72301 acttcttcag ccaattcttc ttttataatt tcttgtgata aagctacgca ttcattgcag
    72361 atgaaaacat tatttcctgc gataattttt ttaacgtcat cctggctttt accacaaaat
    72421 gaacagtata ctttaatatc gttagttcta cttcctgcca tttcttataa ttctcttact
    72481 ttctcttatc ttttactgaa ttttaatatt agtcaatgtg ttgtattggt ttttggtgat
    72541 tcttgcttga tagctaaatc ttcaccagtg ctgtctttgc aagtcgcatc tcaaaacaat
    72601 gtaccataga aggcgtgaat actattaacc agattagtaa acgcatttaa taagaacaga
    72661 cttgctaagc cataacgatt ctttttaaaa gctggaatgg cacaaagaac acaaatcacg
    72721 cacaaaaaag ccgtaaatac tccaaaaata ctacgttgca tcgcttatca tctcctcaga
    72781 ctatttgtgt tttaaagcag taacggtaaa atcataagga tttttagcgt ctcgagcata
    72841 gaacgtttgg ctttcttgcg tgaaatgact aaaatctaga tttggtcggt aggtatctcc
    72901 tttaaaacga tgatggataa ctgtttttat gacacgatcg aaataaccat taaacgcttc
    72961 taaaacctta ttcccaccaa tgatataaag tgttttatcc tgcgcatggt accactccaa
    73021 gactttttca atactagtca ttgtcaacac ctcatctact tgataatctc tatttcttgt
    73081 catgacaagg gtttgacgtt gaggaagtct tttgcaattc attccttcaa aagtcacacg
    73141 gcccattaag attgcttgat gtagcgttgt ctttttaaaa tgttctaact cttttggtaa
    73201 ataccatggt aacttacccg caacaccaat aagccctgct tcatcctctg cccaaatagc
    73261 aataatttcc tttgtcatta attatttcct tcattttaaa ctctatttat tttaccaaat
    73321 ttatgaacaa aatgctcaca aaaaatctaa ataaagttta aatagcaagg tcaaagtgca
    73381 actgaggctt cactggatca taattttgta attcaaaatc atctggttta atatcaaaga
    73441 aattagtcct atctggaaca tttaaaacaa gcttaggctg actagcaact ggctgtcgtt
    73501 ttagcaattc ttgagcctga tcaaattggt tgtcataaat atgtaaattg ttaacaaaat
    73561 aaaagaactt acctattttc catccaaaat gtttagcaat catcatctgc aaggcaacgt
    73621 attgcatggc attgatgtga tgagcaacta atatgtcatt agaacgttgc gtcaaactag
    73681 catctaaata aagatcttcc cctacacgtc taacatcaaa catgatttga aaggcacatg
    73741 gcaacaaccc ttttgtctcc tcaaatgctt catagtccca gagagaaatg acattacgac
    73801 gattccaagg attttctgct aattgcttta gtatttttga gatgatatca tgttttttga
    73861 ctacagcacc ataacgttgc ccaatagtac gtgtctggtc aacttcccat tcattccaat
    73921 aatgaacatt atatttagct tctagcacat ctaaactgtt agactgatct tgatatatcc
    73981 aaaataattc ctttatagca gatttaattg gaatgggtcg taaagttgtg atgggaaact
    74041 ctcctttagc taaatcatat tcagcaaacg ccccagtaat atacttagaa tgagctgttc
    74101 gaccatcttt atatttagga cgtgcttgct cactcaatga tccttcgttt ataatttttt
    74161 gaatattagc tttaaaaatc tggtcagctt ttgtcatctc taataacctt tctgtaagca
    74221 tacttcatac tattctaact aattttacca aaaaatacca gaataaataa acattcgaaa
    74281 aacttcattt ggtcatgaac att
//
LOCUS       DAXWIQ010000003        63147 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000003 DAXWIQ010000000
VERSION     DAXWIQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 63147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63147
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.003"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            41..1936
                     /gene="pepO"
                     /locus_tag="ACGEUI_000162"
     CDS             41..1936
                     /gene="pepO"
                     /locus_tag="ACGEUI_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HFF8165035.1"
                     /translation="MTTYQDDFYQAVNGKWAETAVIPDDKPRTGGFSDLADEIEALML
                     DTTDAWLAGENIPDDAILKNFVKFHRLVADYAKRDEVGVSPILPLIEEYQSLKSFSEF
                     VANIAKYELAGLPNEFPFSVAPDFMNAQLNVLWAEAPSILLPDTTYYEEGNEKAEELR
                     GIWRQSQEKLLPQFGFSTEEIKDLLDKVIELDKQLAKYVLSREEGSEYAKLYHPYVWA
                     DFKKLAPELPLDSIFEKILGQVPDKVIVPEERFWTEFAATYYSEANWDLLKANLIVDA
                     ANAYNAYLTDDIRVESGAYSRALSGTPQAMDKQKAAFYLAQGPFSQALGLWYAGQKFS
                     PEAKADVESKVARMIEVYKSRLETADWLAPATREKAITKLNVITPHIGYPEKLPETYA
                     KKVIDESLSLVENAQNLAKITIAHTWSKWNKPVDRSEWHMPAHLVNAYYDPQQNQIVF
                     PAAILQEPFYSLDQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNDWWTQEDYA
                     AFKERTDKIVAQFDGLESHGAKVNGKLTVSENVADLGGVACALEAAQSEEDFSARDFF
                     INFATIWRMKAREEYMQMLASIDVHAPGELRTNVTLTNFDAFHETFDIKEGDAMWRAP
                     KDRVIIW"
     gene            complement(2102..3142)
                     /gene="tsf"
                     /locus_tag="ACGEUI_000163"
     CDS             complement(2102..3142)
                     /gene="tsf"
                     /locus_tag="ACGEUI_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFF8165036.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREK
                     GMAKAAKKADRVAAEGLTGVYVHGNVAAVVEVNAETDFVAKNAQFVELVNATAKVIAE
                     GKPANNDEALALVMPSGETLAEAYVNATATIGEKISFRRFALIEKADEQHFGAYQHNG
                     GRIGVISVVEGGDDALAKQVSMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNE
                     SRAMVDKPALPFLKYGSKAQLSDDVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLDSVNAKAIAFARFEVGEGIEKKANDFES
                     EVAATMAAALNN"
     gene            complement(3276..4043)
                     /gene="rpsB"
                     /locus_tag="ACGEUI_000164"
     CDS             complement(3276..4043)
                     /gene="rpsB"
                     /locus_tag="ACGEUI_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFF8165037.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVADEATRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFDVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDIIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDADVAFEADTQADSIEEIVEVVEGDNA"
     gene            4347..7421
                     /locus_tag="ACGEUI_000165"
     CDS             4347..7421
                     /locus_tag="ACGEUI_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8165038.1"
                     /translation="MTKGIRFQLLGSPHIFLDDKEQFFAFAKANALLYYLVVNGSVSR
                     EVAASLLWENKNTQTAKKNLRNAIYQVNKVLQADVIICPNRNLLVLNKTLDIKTDINL
                     FLADPLAHLELYQGEFLQGFYLKSGEEFDLWVSKMRMQYEQVYLKACYQKIEEKLSLD
                     AIEDVEEHLKQLIERDEFEEKNYQLLMRLYQQGNCPGKVIETYYQLANVLDKELGIQP
                     SLQSQQIYQEVVAKDRNERKIKHFLRNSNHFLGRIDEIKQLENYFANCLACQEVGALL
                     LIGDTGIGKRTLARQVLANQTQTFQIVTAKCFREEAMDSLLPWRNILDGLGDLVIQNR
                     LLTTKAWKAALKRCFPVATIFQEDNNQPFIKDHTSLLVSFIVDILQHLAEIKALVILI
                     EDCHWMDEDSLTLLQRVMNQLVHYPIAFVLTKHLGTTPELGLCLNALMSQGRLESICL
                     EPFNRQESLVYINSQLGSQPVTAEEMEHLYQASQGNPFFLSEYTQALLRHEKFVPLTP
                     AIKAKLGLKLANLSSRDDALLNYLSCCRRPIPLNTLAQLMLLPLEEVIEMVDNLGHYY
                     ILVEESVGEEVLISFRQRIIQLYSYDRLSLSKRRLLHGQIAKRLEDLLPILTPSPHLL
                     DDIAYHYQESRQVIKALEYNLNYLDATLPFQHELFPIYSKSIGSLEKSDRDHQRLMEE
                     QFDKIRQSIADLELTYDNNRDFQQLLIRFSYLEGRYDIRTGRYQEGIKHIQKVIALAT
                     ELKQPSFLLEGYRQLIHYCIQVENKPEMRYYTSLSLEAAVAANHFEAIAISLRLNGLY
                     HLIIGELNEAERLLQQSIDFFKVTPGLQANYAIQIAAALDYLGEIAQIRYQFEKAVAY
                     QKQAIALTENKPAELSASIFYIGLGISYFYLADFEQAEQILSLAKEALVNHSYPWKET
                     QLEIYLAMIQWKKGNYQPALTLLDYRETLMSRYRNPRDKGLVFYLMAVVKYQLIFQGA
                     TLSQQEKEMADHLLSESFEYYYEIASTNLNPYRDCHLVSELNDLRQQLSAKS"
     gene            complement(7452..8438)
                     /locus_tag="ACGEUI_000166"
     CDS             complement(7452..8438)
                     /locus_tag="ACGEUI_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HFF8165039.1"
                     /translation="MLEDYYPSTTSYYHGGIDDDLYTAKWGMVMTFLDLNDSSLTPFE
                     GTHFALIGFKSDKGVYINNGRVGAVESPAAIRTQLAKFPWHLGNQVMVYDVGNIDGPN
                     RSLEQLQNSLSKAIKRMCDLNLKPIVLGGGHETAYGHYLGLRQSLSPSDDLAVINMDA
                     HFDLRPYDQTGPNSGTGFRQMFDDAVADKRLFKYFVLGIQEHNNNLFLFDFVAKSKGI
                     QFLTGQDIYQMGHQKVCRAIDRFLEGQERVYLTIDMDCFSVGAAPGVSAIQSLGVDPN
                     LAVLVLQHIAASGKLVGFDVVEVSPPHDIDNHTANLAATFIFYLVQIMAQHS"
     gene            complement(8624..10165)
                     /gene="hutH"
                     /locus_tag="ACGEUI_000167"
     CDS             complement(8624..10165)
                     /gene="hutH"
                     /locus_tag="ACGEUI_000167"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HFF8165040.1"
                     /translation="MTRVINLDGESLTIEDVIAIARQGVACHIDDSAIEAVNASRKIV
                     DDIVSEKRVVYGVTTGFGSLCNVSISPEDTVQLQENLIRTHASGFGDPLPEDAVRAIM
                     LIRINSLVKGYSGIRLSTIEKLLELLNKGVHPYIPEKGSLGASGDLAPLAHMVLPMLG
                     LGKAYYKGELLSGQEALDKAGIDKISLAAKEGLALINGTTVLTAVGALATYDAIQLLK
                     LSDLAGALSLEVHNGITSPFEENLHTIRPQSGQLATARNIRNLLEGSQNTTVATQSRV
                     QDPYTLRCMPQIHGASKDSIAYVKSKVDIEINSVTDNPIICKDGHVISGGNFHGEPMA
                     QPFDFLGIAISEIGNVSERRVERLVNSQLSKLPSFLVKYPGLNSGFMITQYACASLAS
                     ENKVLAHPASVDSIPSCENQEDFVSMGTTAARKAFEILKNSRRIVATEIMAACQALDL
                     KPENHELGKGTKVAYDLFRKEVNFIEHDKHIEIYDELNKASAVIEDPSFLEAVEQAVE
                     LSIQF"
     gene            complement(10176..11519)
                     /locus_tag="ACGEUI_000168"
     CDS             complement(10176..11519)
                     /locus_tag="ACGEUI_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8165041.1"
                     /translation="MASNHMNEQEREQAKFSLSGATLYGINAVIGSGIFLLPRAIYKG
                     LGPASIAVMFGTAILTIMLAVCFAEVSGYFGKNGGAFQYSKRAFGDFIGFNVGFLGWT
                     VTIFAWAAMAAGFARMFIITFPAFEGWHIPLSIGLIILLSLMNIAGLKTSKIVTITAT
                     IAKLIPIVAFCACTLFFIKNGLPNFTPFVQLEPGTNLLGAISNTAVYIFYGFIGFETL
                     SIVAGEMRDPEKNVPRALLGSISIVSVLYMLIIGGTIAMLGSQIMMTNAPVQDAFVKM
                     IGPAGAWMVSIGALISITGLNMGESIMVPRYGAAIADEGLLPAAIAKQNQNGAPLVAI
                     LVSGAIAIVLLLTGSFESLAKLSVVFRFFQYIPTALAVMKLRKDDPDANVIFRVPFGP
                     IIPILAVIVSLVMIWGDNPMNFVYGAVGVIIASSVYYLMHGRNHQMDQMPPKEQI"
     gene            complement(11739..12335)
                     /locus_tag="ACGEUI_000169"
     CDS             complement(11739..12335)
                     /locus_tag="ACGEUI_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HFF8165042.1"
                     /translation="MTKVTIKAPSDYLQTDWSGGETNQLFLYPEDGDYKKREFSYRLS
                     TATVALRQTTFTRLDGYHRILMSLDKPITLSDLSHAKEISLAPFQPYAFEGDTRIVSR
                     GTCQDINLMYDDQYQGQLLPVWSDTPFEAPKADCQLLYALSKLTYSVTNQANQELEAN
                     HLLVTEQEASLSDLKVCLNQTSPAQTPVAVWAGLWYKT"
     gene            complement(12357..14030)
                     /locus_tag="ACGEUI_000170"
     CDS             complement(12357..14030)
                     /locus_tag="ACGEUI_000170"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFF8165043.1"
                     /translation="MVLSDIEIANSVTMEPISKVADQLGIDKEALCLYGKYKAKIDAR
                     QLVALKNKPDGKLILVTAISPTPAGEGKTTTSVGLVDALSAIGKKAVIALREPSLGPV
                     FGVKGGAAGGGHAQVVPMEDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSR
                     RITWKRVVDMNDRQLRHIVDGLQGKVNGIPREDGYDITVASEIMAILCLSENISDLKA
                     RLEKIIIGYNYQGEPVTAKDLKAGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANI
                     AHGCNSVLATKLALKYGDYAVTEAGFGADLGAEKFIDIKCRMSGLRPAAVVLVATIRA
                     LKMHGGVPKADLATENVQAVVDGLPNLDKHLANIQDVYGLPVVVAINKFPLDTDAELQ
                     AVYDACDKRGVDVVISDVWANGGAGGRELAEKVVTLAEQDNQFRFVYEEDDSIETKLT
                     KIVTKVYGGKGINLSSAAKRELADLERLGFGNYPICMAKTQYSFSDDAKKLGAPTDFT
                     VTISNLKVSAGAGFIVALTGAIMTMPGLPKVPASETIDIDEEGNITGLF"
     gene            complement(14048..14674)
                     /locus_tag="ACGEUI_000171"
     CDS             complement(14048..14674)
                     /locus_tag="ACGEUI_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HFF8165044.1"
                     /translation="MGLVDLSLTDFAKVLGSDAPAPGGGSAAALSGANGISLTKMVCE
                     LTLGKKKYADYQDIITEIHAKSTALQASLLAAIDKDTEAFNLVSAVFDMPKETDEDKA
                     ARRTAMQKALKTAAQSPFEMMTLMVEALEITATAVGKSNTNAASDLGVAALNLKAGLQ
                     GAWLNVLINLSGIKDEDFVTDYRQKGQALLDKGCHLADDIYTKILDIV"
     gene            complement(14685..15584)
                     /gene="ftcD"
                     /locus_tag="ACGEUI_000172"
     CDS             complement(14685..15584)
                     /gene="ftcD"
                     /locus_tag="ACGEUI_000172"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - L-histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HFF8165045.1"
                     /translation="MAKIVECIPNFSEGQNQAVIDGLVATAKSIPGVTLLDYSSDASH
                     NRSVFTLVGDDQSIQETAFQLVKYASENIDMTKHHGEHPRMGATDVCPFVPIKDITTQ
                     ECVEISKQVAERINRELGIPIFLYEDSATRPERQNLAKVRKGQFEGMPEKLLEEDWAP
                     DYGDRKIHPTAGVTAVGARMPLVAFNVNLDTDNIDIAHKIAKIIRGSGGGYKYCKAIG
                     VMLEDRHIAQVSMNMVNFEKCSLYRTFETIKFEARRYGVNVIGSEVIGLAPAKALIDV
                     AEYYLQVEDFDYHKQILENHLLG"
     gene            complement(15673..17703)
                     /locus_tag="ACGEUI_000173"
     CDS             complement(15673..17703)
                     /locus_tag="ACGEUI_000173"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HFF8165046.1"
                     /translation="MSFYSETDIAAAMTVKLDDVLPEKTVFEEGIRRAPDRGFRLTQA
                     QTEIALKNALRYVPTKFHEEVIPEFLEELKTRGRIYGYRFRPKDRIYGKPIDEYKGNC
                     TAAKAMQVMIDNNLSFEIALYPYELVTYGETGSVCANWMQYCLIKKYLEVMTDEQTLV
                     VESGHPVGLFKSKPEAPRVIITNGLLVGEYDNMKDWEIAEEMGVTNYGQMTAGGWMYI
                     GPQGIVHGTFNTLLNAGRLKLGVADDGDLTGKLFISSGLGGMSGAQGKAAEIAKAVAI
                     IAEVDQSRIKTRHSQGWISQIAESPEEALQLAQKAIDAKESTSIAYHGNIVDLLEYVN
                     DKQIHVDLLSDQTSCHNVYDGGYCPVGISFDERTRLLAEDKDTFHQMVDDTLARHFEA
                     IKTLTENGTYFFDYGNAFMKSVYDSGITEISKNGRNDKDGFIWPSYVEDIMGPMLFDY
                     GYGPFRWVCLSGNHDDLVATDKAAMEAIDPDRRYQDRDNYNWIRDAEKNQLVVGTQAR
                     ILYQDCIGRVTIALKFNELVRKGKIGPVMIGRDHHDVSGTDSPFRETSNIKDGSNVTC
                     DMAVQCYAGNAARGMSLVALHNGGGTGIGKAINGGFGLVLDGSERIDEIIKSAIAWDT
                     MGGVARRNWARNEHAIETAIEYNRLHAGTDHITIPYLADDDLVTSAVDQLFH"
     gene            17995..19260
                     /gene="hutI"
                     /locus_tag="ACGEUI_000174"
     CDS             17995..19260
                     /gene="hutI"
                     /locus_tag="ACGEUI_000174"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0019556 - L-histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HFF8165047.1"
                     /translation="MVADVLLTHFNQLFCLNDPGHPLTGQEMKKATIVEDGYIAIKDG
                     LIVALGSGEPDAELVGTQTIMRSYKGKIATPGIIDCHTHLVYGGSREHEFAKKLAGVS
                     YLDILAQGGGILSTVRATRSASFDNLYQKSKRLLDYMLLHGVTTVEAKSGYGLDWETE
                     KRQLDVVAALEKDHPIDLVSTFMAAHAIPEEYKGNPKAYLDVIIKDMLPVVKEENLAE
                     FCDIFCEKNVFTADESRYLLSKAKEMGFKLRIHADEIASIGGVDVAAELSAVSAEHLM
                     MITDDGIAKLIGAGVIGNLLPATTFSLMEDTYAPARKMIDAGMAITLSTDSNPGSCPT
                     ANMQFVMQLGCFMLRLTPIEVLNAVTINAAYSVNRQERVGSLTVGKEADIAIFDAPNI
                     DYPFYFFATNLIHQVYKKGQLTVDRGRIL"
     gene            complement(19318..20850)
                     /gene="ahpF"
                     /locus_tag="ACGEUI_000175"
     CDS             complement(19318..20850)
                     /gene="ahpF"
                     /locus_tag="ACGEUI_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFF8165048.1"
                     /translation="MALSPDIKEQLAQYLTLLEADLVLQVSLGDNEQSQKVKDFVEEI
                     AAMSERISIENITLDRQPSFKVAKKGHGSGVVFAGLPLGHELTSFILALLQVSGRAPK
                     VDQDVIDRIKAIDRPLHFETYVSLTCHNCPDVVQALNIMSVLNDKISHTMVEGGMFQD
                     EVKAKGIMSVPTVFLDGEEFTSGRATIEQLLEQIAGPLSEEAFADKGLYDVLVIGGGP
                     AGNSAAIYAARKGLKTGLLAETFGGQVMETVGIENMIGTLYTEGPKLMAEVEAHTKSY
                     DVDIIKAQLATSIEKKENIEVTLANGAVLQAKTAILALGAKWRNINVPGEDEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGLEAALDLAGLAKHVYVLEFLPELKADKVLQDRA
                     AKTNNMTIIKNVATKDIVGEDHVTGLNYTERDSGEDKHLDLEGVFVQIGLVPNTAWLK
                     DSGVNLTDRGEIIVDKHGSTNIPGIFAAGDCTDSAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(20871..21431)
                     /gene="ahpC"
                     /locus_tag="ACGEUI_000176"
     CDS             complement(20871..21431)
                     /gene="ahpC"
                     /locus_tag="ACGEUI_000176"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFF8165049.1"
                     /translation="MSLIGKEIAEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLGVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQAFEVLGEDGLAQRGTFIVDPDGIIQMMEINADGIGRDASTLIDKIHAAQYVRKH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            21837..21907
                     /locus_tag="ACGEUI_000177"
     tRNA            21837..21907
                     /locus_tag="ACGEUI_000177"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21869..21871,aa:Cys,seq:gca)
     gene            22427..22630
                     /locus_tag="ACGEUI_000178"
     CDS             22427..22630
                     /locus_tag="ACGEUI_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8165050.1"
                     /translation="MAQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQTNGFKTLEEG
                     QKVAFDVEEGQRGPQAVNITKLA"
     gene            22826..23287
                     /locus_tag="ACGEUI_000179"
     CDS             22826..23287
                     /locus_tag="ACGEUI_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFF8165051.1"
                     /translation="MPTKNTSDSIEEYIKELLAKSGIAEIKRSMLADSFQVVPSQINY
                     VIKTRFTESRGYEVESKRGGGGYIRIAKVHFSDKHHLIGNLMATIEDCISEQVFTDSI
                     QLLFDEHLLTEREGNIILAVASDDVLGTDGSTIRARMLYRLLQRIDRKGSN"
     gene            23287..25731
                     /locus_tag="ACGEUI_000180"
     CDS             23287..25731
                     /locus_tag="ACGEUI_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFF8165052.1"
                     /translation="MIMYSTKMQDIFRQAQFQAARFDSHCLETWHVLLAMVAVDNSLA
                     NMILSEYDAQVAIEEYEAAAILAMGKTPKEQLSRVDFRPQSKTLTNLLAFAQAISQIT
                     RDQEVGSEHVLFAILLNPDIMASRLLEIAGYQIKDNGNGQPRLADLRKAIERHAGYSK
                     EMIKAIHELRKPKKTKTQGTFSDMMKPPSTAGELSDFTRDLTEMARQGLLESVIGRDQ
                     EVSRMIQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGAIPYELKEMRVLELDMM
                     SVVAGTRFRGDFEERMNQIIDDIEADGQIILFVDELHTIMGSGSGIDSTLDAANILKP
                     ALSRGTLHMVGATTQEEYQKHIEKDAALSRRFAKILIEEPNTEDAYQILMGLKLSYET
                     YHNVSISNEAVKTAVKMAHRYLTSKNLPDSAIDLLDEASAAVQNMVKKSAPETLTPID
                     QALINGDMKKVSRLLAKEAKGQMRKPTPVTEDDILATLSKLSGIPLEKLTQADSKKYL
                     NLEKELHKRVIGQDAAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKAL
                     AEVLFDDEAALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTQKVRNKPYSVLLF
                     DEVEKAHPDIFNVLLQVLDDGILTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFG
                     VKDIHQDHQAMEKRILEELRKTYRPEFINRIDEKVVFHSLTQDNMRDVVKIMVQPLIT
                     TLAEKGITLKIQPLALKHLSEVGYDEHMGARPLRRTLQTEIEDKLSELILSRELTSGH
                     TLKIGLSHGKLTFHIA"
     gene            25909..26199
                     /gene="groES"
                     /locus_tag="ACGEUI_000181"
     CDS             25909..26199
                     /gene="groES"
                     /locus_tag="ACGEUI_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFF8165053.1"
                     /translation="MLKPLGDRVVVRFDDEKEQTVGGFVLAGTHKESTRKATVLAVSE
                     TGVRTITGDSVLPSVSVGQEVLVENGHDLEVTVDDEKVSIIRESDIIAIVTK"
     gene            26235..27866
                     /gene="groL"
                     /locus_tag="ACGEUI_000182"
     CDS             26235..27866
                     /gene="groL"
                     /locus_tag="ACGEUI_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFF8165054.1"
                     /translation="MAKDIKFSADARAAMVRGVDMLADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVHEG
                     LKNVTAGANPIGIRRGIETATATAVEALKAIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVADLENPFI
                     LITDKKVSNIQDILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATMTALGQAAKITVDKDSTVIVEGS
                     GSSEAIANRIALIKSQLETTTSDFDREKLQERLAKLAGGVAVIKVGAPTETALKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALITVIEKVAALELEGDDATGRNIVLRALEEPVRQ
                     IALNAGYEGSVVIDKLKNSPAGTGFNAATGEWVDMIKTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPATPAPAMPAGMDPGMMGGF"
     gene            28117..28402
                     /locus_tag="ACGEUI_000183"
                     /pseudo
     CDS             28117..28402
                     /locus_tag="ACGEUI_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004183195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(28394..28775)
                     /locus_tag="ACGEUI_000184"
                     /pseudo
     CDS             complement(28394..28775)
                     /locus_tag="ACGEUI_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920900.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
     gene            complement(29069..30565)
                     /locus_tag="ACGEUI_000185"
     CDS             complement(29069..30565)
                     /locus_tag="ACGEUI_000185"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HFF8165055.1"
                     /translation="MINKKISLGVLSILTAFSLQSVSYACTGFIIGKDLTKDGSLLYG
                     RTEDLEPHHNKNFIVRLAKDNPAGEKWKDLSNGFEYPLPEHSYRYSAIPDVTPNKGVY
                     DEAGFNEFGVSMSATVSASANDAIQKIDPYVKNGLAESSMTSVILPSVKTAREGVALI
                     AKIVTEKGAAEGNIVTLADKDGIWYMEILSGHQYVAIKFPDDKYAVFPNTFYLGHVDF
                     NDKENTIASEDVEKVAKKAKSYTEVDGKFHIAKSYNPPLNDANRSRSFSGIKSLDPDS
                     KVTYKDSNYELLQSTDKTFSLEDAMKLQRNRFEGLDLKPLDQMALDGKGKPKSKKAVK
                     GYAYPISNPNVMEAHIFQLKKDIPAELGGVMWLSIGSPRNAPYLPYLGNISRTYEAYQ
                     EKSTQYNDKSWYWTVSHINDLVAAHPKPFGTKVIDEMKGLEKTWIAEQDKSTKEISDL
                     VVSDPKAAQEKADKISLDRAEKTFKRLKAIEAKLVKEKPKNKKGLNRS"
     gene            30812..32452
                     /locus_tag="ACGEUI_000186"
     CDS             30812..32452
                     /locus_tag="ACGEUI_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFF8165056.1"
                     /translation="MTKPFHHKKLKQITIIAATSLFLFLIGGAFYYSKNHCINAYLKA
                     RSAQSGPVFENIKAYLVWDDTNEQITNDEAMYTKFRRYSQKELRQKKQDLKAASQDSA
                     VQVKSVGRRFWIFPDYRIAIKPMDLTIKTNVPQADVLLNQKKVAVSDSEQFSVKLDRL
                     PTAEYTASIRGKHNGRNIKVNKSYDGDNPVLDLSVSFRTFLVTSNAKQGDLYFDDNHI
                     GTLKDGQLQVEDYPVTENAQAYMKTTFPDGELRSQKYALADVEEGATLEILVTDLLEE
                     DKAGELLVSAFDQLMHYLSTGQDSSNLRSVFEAGSSNAFYRGLKESIKAKFQTDTRKA
                     SRLNIPSILLTTMTQVGKTTYVLDFTATYEFLYDKSTDPEQHTSGHINQDLTGKVTVK
                     KVGQHYLISQSGSKNITVVKEDNQLKAPSVFPESILGTWTGQANGLSIHMSLASDGTI
                     TTKVEDQKGNRSKETRTAKISKVEDKGNGFYLYTPDPGSDISALVPEGGLGGANVKYA
                     YGFKISGKTASPVVWQAALTHEFDYTKPLSGVTLQKQP"
     gene            complement(32541..33452)
                     /locus_tag="ACGEUI_000187"
     CDS             complement(32541..33452)
                     /locus_tag="ACGEUI_000187"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8165057.1"
                     /translation="MKTTNKPSKGFTVTLKNPFEELTVKALLEEQLLIPRKIRHFLRT
                     KKHVLINGHSVNWQSCVKYGDQVKLFFDHEDYPEKIIVMGQAEKVTCLYEDEHIIIVN
                     KPEGMKTHGNDPTELALLNHVSAYTGQTCYVVHRLDKETSGAILFAKTPFILPILNRL
                     LEKRDIHREYLALVHGSLDSPRVTYHHPIGRHRHDRRKRVVDPINGKKAITEVTLVKN
                     FHKTASLLTCQLQTGRTHQIRVHLAHQGHVLFGDPLYSNGKKDCARLMLHAYQLRLKH
                     PLTQEDICVQAKSATFDAVLNAQKARK"
     gene            complement(33436..33627)
                     /locus_tag="ACGEUI_000188"
     CDS             complement(33436..33627)
                     /locus_tag="ACGEUI_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165058.1"
                     /translation="MNKFQAFKETLSAESLKAIYDETRLEVANDEREGTEAFSAALAT
                     QMAINLVEKYHNWLNEDNK"
     gene            34118..34498
                     /locus_tag="ACGEUI_000189"
     CDS             34118..34498
                     /locus_tag="ACGEUI_000189"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFF8165059.1"
                     /translation="MKTIRRYDVNEDRGHTGLVEAGDFYYLNYCVGNVGQDIESQING
                     AFDEMERRLALVGLTLDAVVQMDCLFRDVWNIPVMEKMIKERFNGRYPARKSIQTEFA
                     HHGGPQGLLFQVDGVAYSKHISMT"
     gene            34537..36873
                     /gene="pbp2a"
                     /locus_tag="ACGEUI_000190"
     CDS             34537..36873
                     /gene="pbp2a"
                     /locus_tag="ACGEUI_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2A"
                     /protein_id="HFF8165060.1"
                     /translation="MRFLELLQKKFFPKAYQEKQFLMHQKTRLTPQHNQKQYSPNANH
                     LDSSATKNSEQDPATALQRSRAYEGSPKSRPAWLQKLEAVLPSPQRPIRRFWRRYHIG
                     KLLMILIGTLVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSY
                     VELNAISDDLENAVIATEDRTFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAY
                     LSQDQTIKRKAREFFLALELTKKYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTA
                     ANLTLDEAATLAGMLKGPEIYNPYHSLKNATHRRDTVLGAMVDAKKITQTKAQQARAV
                     GLKNRLADTYVGKTDDYKYPSYFDAVISEAIATYGLSEKDIVNNGYKVYTELDQNYQT
                     GMQTTFNNDELFPVSAYDGSSAQAASVALDPKTGGVRGLIGRVNSSENPTFRSFNYAT
                     QAKRSPASTIKPLVVYAPAVASGWSIEKELPNTVQDFDGYQPHNYGNYESEDVPMYQA
                     LANSYNIPAVSTLNDIGIDKAFTYGKTFGLDMSSAKKELGVALGGSVTTNPLEMAQAY
                     AAFANNGVIHPAHLINRIENARGEVLKTFTDKAKRVVSQSVADKMTAMMLGTFSNGTA
                     VNANVYGYTLAGKTGTTETNFNPDLAGDQWVIGYTPDVVISQWVGFNQTDENHYLTDS
                     SAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISAVYGVNETGNQSGVDTQSIID
                     GLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR"
     gene            36922..37074
                     /gene="rpmG"
                     /locus_tag="ACGEUI_000191"
     CDS             36922..37074
                     /gene="rpmG"
                     /locus_tag="ACGEUI_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8165061.1"
                     /translation="MAQKKASLACVECGSRNYSIGVSSTPKPTRLEVNKFCKYCKTYT
                     LHKETR"
     gene            37088..37264
                     /gene="secE"
                     /locus_tag="ACGEUI_000192"
     CDS             37088..37264
                     /gene="secE"
                     /locus_tag="ACGEUI_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFF8165062.1"
                     /translation="MGFISGTFKVLKDTTWPNRKQRWKDFISVLEYTAFFTVIIYIFD
                     QLLAKSVLALINLF"
     gene            complement(37501..38274)
                     /locus_tag="ACGEUI_000193"
     CDS             complement(37501..38274)
                     /locus_tag="ACGEUI_000193"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFF8165063.1"
                     /translation="MTDRGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQQ
                     KAPEQMLTSDGLNTKIVGEEKTVDEVIEEVLKDLDFYEESGGGMTLSGGEIFAQFDFA
                     LALLKAAKAAGLHTAIETTAFAKHEQFVTLVDYVDFIYTDLKHYNQLRHQKVTGVRND
                     LIIKNIHYAFQAGKEIVLRIPVIPQFNDSLDDAKAFSELFNQLEIDQVQLLPFHQFGE
                     NKYKLLGREYEMAEVKAYHPEDLADYQAVFLNHNIHCYF"
     gene            38400..39146
                     /locus_tag="ACGEUI_000194"
     CDS             38400..39146
                     /locus_tag="ACGEUI_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019317626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8165064.1"
                     /translation="MNRLERIIQLVSQKKKIDVNSLSEQLDVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNVRLSYNYNIKRRIAEKAAELVQDNDTIMIESGSTCALLAE
                     VLCQTKRNIKVITNSCFIANYIRQYSSCQIILLGGYYQPNSEVTVGPLLKEMISLFHV
                     NRVFVGTDGFNKDLGFMGKDMMRSEGVRYMADAAEEVVILTDSSKFSKTSLVHQLSLA
                     DVNRVITDQALDKQTQELLSASGLVLDFVS"
     gene            39162..40142
                     /locus_tag="ACGEUI_000195"
     CDS             39162..40142
                     /locus_tag="ACGEUI_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFF8165065.1"
                     /translation="MKEERRRLLAKVAYLHYVQGKSQTLISKEMNIYRTTVCRMLAKA
                     KEEGIVRIEIADYDADLFALEEYVRQQYGLEKLDLVPNQVEDTPMDTLTNVAKTAAEV
                     FRHVVKDGDKIGLSWGATLSCLMDELNPKAMKDVFIYPLAGGPSHINAKYHVNTLVYR
                     LARIFHGNSAFMNAMVIQEDKHLAKGILQSKYFNDILTSWDQLDLALVGIGGEPNSLE
                     QSQWRDLLTSSDHDQLKYEKAVGEVCCRFFDQAGQPVYTGLQDRTIGISLEQLRRVPK
                     TMAVATGKHKAKAILAALKAGFINYLVTDKETMLAVLALDEDIDLNNVLL"
     gene            40430..40750
                     /locus_tag="ACGEUI_000196"
     CDS             40430..40750
                     /locus_tag="ACGEUI_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8165066.1"
                     /translation="MQVIVPDQIIMGLILNAGDAKQHIYQALKCAKEDDYATSEKEMA
                     LADDALLEAHNLQTQFLAQEASGNKSEITALFVHSQDHLMTTITEINLIKEIIDLRKE
                     LATK"
     gene            40778..41086
                     /locus_tag="ACGEUI_000197"
     CDS             40778..41086
                     /locus_tag="ACGEUI_000197"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8165067.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKVAAEKKGIDCQIEAYAQGKLAD
                     YAPLLDVALLGPQVAYTLDKSEAICKDNDIPIAVIPMADYGMLDGNKVLDLALSLVKE
                     "
     gene            41096..42400
                     /locus_tag="ACGEUI_000198"
     CDS             41096..42400
                     /locus_tag="ACGEUI_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8165068.1"
                     /translation="MAKMNMQNIIKPIMTFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     AGQIPFQGVNDAIASVFGADWTEPFMQVYHGTFAIMGLISCFAIGYSYAKNSGVEPLP
                     SGVLSLSAFFILLRSSYVPAEGEAIGDAISKVWFGGQGIIGAIVIGLTVGAVYTTFIR
                     RHIVIKMPDQVPQAIAKQFEAMIPAFVIFTLSMLVYIIAKSVTGGGTFIEMIYDVIQV
                     PLQGLTGSLYGALGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKALMAAGELS
                     LDKGAHIVTQQFLDSFLILSGSGITFGLVVAMIFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFILVPVLAALTVYGAIAIGFMQPFAGVTLPWSTPAIISGFM
                     VGGWQGAIVQILILIMSTLVYFPFFKIQDNMAYQNEQASEES"
     gene            42610..45027
                     /locus_tag="ACGEUI_000199"
     CDS             42610..45027
                     /locus_tag="ACGEUI_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFF8165069.1"
                     /translation="MTETKSPYFGHLTDRMTHYREAVLDKKPYIDAERAILATEAYQK
                     HQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLEFVLNELD
                     LFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGK
                     MNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAV
                     KTYAKRYAKLARELAKTAKPERQAELLDIARICDKVPYEPAKTFAEAVQSVWFIQCIL
                     QIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDTIVERLTNLWIKTLTINKVRSQAH
                     TFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNT
                     FMNECIEVMKLGFGMPAMNNDEIIIPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYR
                     CTGMSYINFPKILLITMNDGIDPASGKRFAKGHGHFKDMTSYEELKAAWDATLREITR
                     MSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVGIANLSD
                     SLAALKKLVFEEGRLTPEELWKALESDFAGERGEDIRQMLINDAPKYGNDDDYADSLV
                     VEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGKGTLATPDGRHAGTPL
                     AEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLKLMALL
                     RTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDII
                     ERTEHTL"
     gene            45040..45708
                     /locus_tag="ACGEUI_000200"
     CDS             45040..45708
                     /locus_tag="ACGEUI_000200"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFF8165070.1"
                     /translation="MEYMLDTLDLEAIKKWHHILPLAGVTSNPSIAKKEGEIDFFERI
                     REVRAIIGDKASIHVQVIAQDYEGILKDAAEIRRQCGDSVYVKVPVTTEGLAAIKTLK
                     AEGYHITATAIYTTFQGLLAIEAGADYLAPYYNRMENLNIDPEAVIEQLAEAINRENA
                     NSKILAASFKNVAQVNKSFALGAQAITAGPDVFEAGFAMPSIQKAVDDFGKDWEAVHH
                     RKSI"
     gene            45765..46853
                     /locus_tag="ACGEUI_000201"
                     /pseudo
     CDS             45765..46853
                     /locus_tag="ACGEUI_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
     gene            complement(46935..47444)
                     /locus_tag="ACGEUI_000202"
     CDS             complement(46935..47444)
                     /locus_tag="ACGEUI_000202"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFF8165071.1"
                     /translation="MSTDHQLREVSYQLLAERGVSLEAIAELVLFLQNDYIPNLTMAE
                     CLESVEAVLAKREVQNAIITGVELDKLAEANQLSEPLLSILKTDQGLYGIDEILALSI
                     VNLYGSIGFTNYGYLDKTKPGIVDKLNHKDGYSCHTFLDDIVSAIAAAAASRIAHNDP
                     AKSAITNQQ"
     gene            47808..48623
                     /gene="sdaB"
                     /locus_tag="ACGEUI_000203"
     CDS             47808..48623
                     /gene="sdaB"
                     /locus_tag="ACGEUI_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptodornase B"
                     /protein_id="HFF8165072.1"
                     /translation="MNLLGSRRVFSKKCRLVKFSMVALVSATMAVTTVTLENTALARQ
                     TQVSNDVVLNDGASKYLNEALAWTFNDSPNYYKTLGTSQITPALFPKAGDILYSKLDE
                     LGRTRTARGTLTYANVEGSYGVRQSFGKNQNPAGWTGNPNHVKYKIEWLNGLSYVGDF
                     WNRSHLIADSLGGDALRVNAVTGTRTQNVGGRDQKGGMRYTEQRAQEWLEANRDGYLY
                     YEAAPIYNADELIPRAVVVSMQSSDNTINEKVLVYNTANGYTINYHNGTPTQK"
     gene            complement(48864..49706)
                     /gene="ropB"
                     /locus_tag="ACGEUI_000204"
                     /gene_synonym="rgg1"
     CDS             complement(48864..49706)
                     /gene="ropB"
                     /locus_tag="ACGEUI_000204"
                     /gene_synonym="rgg1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982409.1"
                     /note="RopB (Regulator of Protease B) is a paralog of the
                     quorum-sensing transcriptional regulators Rgg2 and Rgg3,
                     which bind short, hydrophobic peptides encoded by genes in
                     their respective vicinities. RopB binds the leaderless
                     short peptide SIP (SpeB-inducing peptide), with sequence
                     MWLLLLFL; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     RopB/Rgg1"
                     /protein_id="HFF8165073.1"
                     /translation="MEIGETVEFIRHSKNISIKQVCGDYLTRQTYYRFIKNNLDISSK
                     KLLYILDNLNVNVDEFLFISNNFKQYKEFIDMDTAKHYFECRNIEGLNHILDSYKDSK
                     STKEKNLFALVKVLLATLTEEDCLTERTYLSNYLINIETWSHYETVLFNNCMFIFESC
                     FIEMVFSKVILNLDKYNTLRYYGNESIRMFVNMLILFIQRQEYDKASEILAKIEDYQL
                     NDDCLYERCCVSFFDGIIGLINGKEGAEQKCVQILEIFQLLNCKTIHHMFQTYLEAIK
                     HKLS"
     gene            50646..51842
                     /gene="speB"
                     /locus_tag="ACGEUI_000205"
     CDS             50646..51842
                     /gene="speB"
                     /locus_tag="ACGEUI_000205"
                     /EC_number="3.4.22.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine proteinase exotoxin SpeB"
                     /protein_id="HFF8165074.1"
                     /translation="MNKKKLGVRLLSLLALGGFVLANPVFADQNFARNEKEAKDSAIT
                     FIQKSAAIKAGARSAEDIKLDKVNLGGELSGSNMYVYNISTGGFVIVSGDKRSPEILG
                     YSTSGSFDANGKENIASFMESYVEQIKENKKLDTTYAGTAEIKQPVVKSLLDSKGIHY
                     NQGNPYNLLTPVIEKVKPGEQSFVGQHAATGCVATATAQIMKYHNYPNKGLKDYTYTL
                     SSNNPYFNHPKNLFAAISTRQYNWNNILPTYSGRESNVQKMAISELMADVGISVDMDY
                     GPSSGSAGSSRVQRALKENFGYNQSVHQINRGDFSKQDWEAQIDKELSQNQPVYYQGV
                     GKVGGHAFVIDGADGRNFYHVNWGWGGVSDGFFRLDALNPSALGTGGGAGGFNGYQSA
                     VVGIKP"
     gene            51844..52149
                     /locus_tag="ACGEUI_000206"
     CDS             51844..52149
                     /locus_tag="ACGEUI_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spi family protease inhibitor"
                     /protein_id="HFF8165075.1"
                     /translation="MEMHFVRTEPEARRIAETFCAENTQTKTPMRVQQLSYPSDTDHS
                     GGELYIYALSPAGFIIVSGDTRAHTILGYSFDNNLDLNHDNVRSMIEAYQKQINSLD"
     gene            52629..53558
                     /gene="prsA"
                     /locus_tag="ACGEUI_000207"
     CDS             52629..53558
                     /gene="prsA"
                     /locus_tag="ACGEUI_000207"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HFF8165076.1"
                     /translation="MKQMNKLITGVVTLATVVTLSACQSSHNNTKLVSMKGDTITVSD
                     FYNETKNTELAQKAMLSLVISRVFETQYANKVSDKEVEKAYKQTADQYGTSFKTVLAQ
                     SGLTPETYKKQIRLTKLVEYAVKEQAKNETISKKDYRQAYDAYTPTMTAEIMQFEKEE
                     DAKAALEAVKAEGADFAAIAKEKTTAADKKTTYTFDSGETTLPAEVVRAASGLKEGNR
                     SEIITALDPATSKRTYHIIKVTKKATKKADWKAYQKRLKDIIVTGKLKDPDFQNKVIA
                     KALDKANVKIKDKAFANILAQFAKPNQKQPAQK"
     gene            complement(53834..54070)
                     /locus_tag="ACGEUI_000208"
     CDS             complement(53834..54070)
                     /locus_tag="ACGEUI_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165077.1"
                     /translation="MTHRFFLTPLSTLWRRLRPKRYSKKVLADEPTSYQPPAAHGPCD
                     DIHQLLEDKSLSDDYLIATSAMRHLWYWGYSQIG"
     gene            54314..54610
                     /locus_tag="ACGEUI_000209"
     CDS             54314..54610
                     /locus_tag="ACGEUI_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFF8165078.1"
                     /translation="MTKQDQLIVEKMEQTYEAFSPKLANLIEALDAFKEHYEEYATLR
                     NFYSSDEWFRLANQPWDDIPCGVLSEDLLFDMIGDHNQLLADILDLAPIMYKHM"
     gene            54757..55041
                     /locus_tag="ACGEUI_000210"
     CDS             54757..55041
                     /locus_tag="ACGEUI_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFF8165079.1"
                     /translation="MKFKKVLVIPALALAATCFLTACGTKKDSKKEEVKEIKMSDIKD
                     DAVSKKTKVVDGEEVTEYTTKDGNVIQIPAGNEEGMESKDAGGSGAPAKN"
     gene            55464..56732
                     /locus_tag="ACGEUI_000211"
     CDS             55464..56732
                     /locus_tag="ACGEUI_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFF8165080.1"
                     /translation="MFQLRKKMTRKQLALLSAGVLTCVVGGSYLIMNHQQQEIVSSVN
                     KVKALTIKEAMEQGKDISLTLAGEVTANNSSKVKIDSSKGEVKEVFVKKGDVVKVGQP
                     LFSYETSQRLTAQSSEFDVQTKANQLQVAKTNAALKWETYNRKVNEINTLKSRYNTAP
                     DESLLEQIRSAEDSVSQALSDAKTADSDVKTAQIELDKANATATTEKGKLEYDTVKSD
                     TAGTIVSLNTDLPNQSKSKKENETFMEIIDKSKMLVKGNISEFDRDKLKIGQKVEVID
                     RKDNSKKWTGKVTQVGNLKAEEKGQGQGQGGNDQQDNPNQAKFPYVIELDQSDKQPLI
                     GSHTYVNVLNNVPEAGKIVLKETFTMAENGKTYVWKVDKNKVKKQEIKTKPFSKGYVE
                     VTSGLTMQDKIAQPLPGMKDGMEVGSIVKP"
     gene            56719..57393
                     /locus_tag="ACGEUI_000212"
     CDS             56719..57393
                     /locus_tag="ACGEUI_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8165081.1"
                     /translation="MLNLKDIRKSYHLGTEEFAILKGIDLEVNEGDFLAIMGPSGSGK
                     STLMNIIGCLDKPGSGSYAIEGRDVSSLSDNELADLRNQKIGFVFQNFNLMPKLTACQ
                     NVELPLTYMNVPKKERRKRALEMLKLVGLEERSEFKPMELSGGQKQRVAIARALVTNP
                     SFILGDEPTGALDTKTSVQIMDLFKQFNDNGKTIIIITHEPEVAALCKKTVILRDGNI
                     EHSDIE"
     gene            57406..58623
                     /locus_tag="ACGEUI_000213"
     CDS             57406..58623
                     /locus_tag="ACGEUI_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8165082.1"
                     /translation="MEDIIAALSSILSHKMRSILTMLGIIIGIGAIIAIFSIIEGNTE
                     NTKRQLIGGSNNTINIVFNKKSSIDPKFPDKSNAKKPDYLPFMAEEELSKIQQVKGVK
                     NALISYGIDDKVYHLGQKSSAKISAITKNVAEVRRMTFIKGSDFSDKDFIDQKQVIYL
                     EKSLYESLFPKDDGLGKFVEVMGNPFRVIGVFESKEQSGLTSGTEKIAYIPLHQWYNI
                     NGVVDATPEITIQTYRADDLKPVAKRVSDMLNQTIPKSDYMFGVMNLKEFERQLDNLN
                     KSNFVLLAGIASISLIVGGIGVMNIMLVSVTERTREIGIKKALGARRKLILKQFLIEA
                     VILTLLGGVIGVISGMVSGLIITRSLEYPYILSLFSVVLSLAFCCIIGIVFGLLPAIK
                     ASKLDPIEALRFE"
     gene            58717..59370
                     /locus_tag="ACGEUI_000214"
     CDS             58717..59370
                     /locus_tag="ACGEUI_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8165083.1"
                     /translation="MFKILVVEDDDTISQVICEFLKANNYDPDCVFDGQAALDKWQTT
                     SYDLIILDIMLPSLSGLEVLKTIRKTSDVPIIMLTALDDEYTQLVSFNHLISDYVTKP
                     FSPLILIKRIENVLRVSTPDEKRQIGDLLVDETEHSVYWQGTLVKLTKKEYDIIDYLA
                     KRHQKIVTRDQLMDDIWGYSELDTRVLDNHIKNLRKKMTGIPLKTITGMGYLLGERE"
     gene            59367..60755
                     /locus_tag="ACGEUI_000215"
     CDS             59367..60755
                     /locus_tag="ACGEUI_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8165084.1"
                     /translation="MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKC
                     EFDQTSKQIKGKTVTEIRDILTKKINKDNIWYSLVDSDNQLLYPSLQLLDGVSESKDS
                     QNVNIVTTFDNSYSNVKVMSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLI
                     FSVTVGSIVAYLYSRTSSRRILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRL
                     YASLSTSIKSLQKEYEKASDSEREKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFA
                     DRDKYLRKCRDVLEGQAQLVQSILSLSKIETLASQNQELFSLKSSLEEEMEVFLVLSE
                     LKHLKVTINLEEQFVKANKVYLLKAIKNIIDNAFHYTKSGGQVMIQLKDNQLVIKNEA
                     ETLLTQQQMKQLFQPFYRPDYSRNRKDGGTGLGLFITHQILDQHHLAYRFVVLDQRWM
                     VFTIDFPSHHDD"
     gene            60857..62458
                     /locus_tag="ACGEUI_000216"
     CDS             60857..62458
                     /locus_tag="ACGEUI_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8165085.1"
                     /translation="MKKRKLLAVTLLSTILLNSAVPLVVADTSLRNSTSSTDQPTTAD
                     TDTDDESETPKKDKKSKETASQHDTQKDHKPSHTHPTPPSNDTKQTDQASSEATDKPN
                     KDKNDTKQPDSSDQSTPSPKDQSSQKESQNKDGRPTPSPDQQKDQTPDKTPEKGPEKA
                     TDKTPEPNRDAPKPIQPPLAAAPVFIPWRESDKDLSKLKPSSRSSAAYVRHWTGDSAY
                     THNLLSRRYGITAEQLDGFLNSLGIHYDKERLNGKRLLEWEKLTGLDVRAIVAIAMAE
                     SSLGTQGVAKEKGANMFGYGAFDFNPNNAKKYSDEVAIRHMVEDTIIANKNQTFERQD
                     LKAKKWSLGQLDTLIDGGVYFTDTSGSGQRRADIMTKLDQWIDDHGSTPEIPEHLKIT
                     SGTQFSEVPVGYKRSQPQNVLTYKSETYSFGQCTWYAYNRVKELGYQVDRYMGNGGDW
                     QRKPGFVTTHKPKVGYVVSFAPGQAGADATYGHVAVVEQIKEDGSILISESNVMGLGT
                     ISYRTFTAEQASLLTYVVGDKLPRP"
     gene            62537..62788
                     /locus_tag="ACGEUI_000217"
     CDS             62537..62788
                     /locus_tag="ACGEUI_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165086.1"
                     /translation="MIKKVTTPSQKTKKRVRNGYLLKLGTACLLLSILSYGIGLLGQP
                     SMENTFMGIASVAMLGSVCFFIIFALNRIFDALEDNLRD"
     gene            complement(62894..63079)
                     /locus_tag="ACGEUI_000218"
     CDS             complement(62894..63079)
                     /locus_tag="ACGEUI_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165087.1"
                     /translation="MVQPLKILSYFSQFTPTLALENVSTMFFMNWLTCLFSLAILRKQ
                     GFGFYFAFNFSHVTLTS"
BASE COUNT        19668 a        12474 c        13259 g        17746 t
ORIGIN      
        1 taaagattct acaaatttaa aatgatataa ggagatacga atgacaactt atcaagatga
       61 tttttaccag gctgtgaatg gaaagtgggc tgaaacagca gttattccag acgacaagcc
      121 tcgaacaggt ggattttcag acttagcaga tgaaattgag gcattaatgc tagacactac
      181 agatgcttgg ctagctggag aaaatatccc tgatgatgct attttaaaaa attttgtgaa
      241 attccatcgt ttggtggctg actacgctaa acgtgatgaa gttggagtaa gtccaattct
      301 ccctttaatt gaagaatatc agtccttaaa atctttttca gaatttgttg ctaatatagc
      361 gaaatatgaa ttggcaggtt taccaaatga atttcccttt agtgtggcac cagactttat
      421 gaatgctcag ctgaatgtgc tttgggcaga agcaccaagc attcttttgc cagatacaac
      481 atactacgaa gaaggcaacg aaaaagctga ggaattgcgt ggtatttggc gtcagtcgca
      541 agaaaagctt ttacctcaat ttggtttttc aacagaagaa atcaaggatc ttttggataa
      601 ggtaattgag ctagataagc aattggccaa gtatgtcttg tctcgtgagg aaggctcaga
      661 atatgccaaa ttataccatc cttatgtgtg ggctgatttt aaaaaactag ctccagagct
      721 tcctttggat agcatttttg aaaagatttt aggccaagta cctgacaagg ttattgttcc
      781 tgaggaacgt ttttggacag aatttgcagc cacttattac tctgaagcca attgggattt
      841 actcaaggct aatttgattg tggacgcggc taatgcttac aatgcttact tgacggatga
      901 tattcgtgtg gaatcaggcg cttattcgcg cgctctttca ggaacacctc aagctatgga
      961 caaacaaaaa gcagcctttt atttggcgca aggaccgttt agccaagcgc ttggcttgtg
     1021 gtatgctggt caaaaattct ccccagaagc taaggctgat gtggaaagta aggtagcacg
     1081 catgattgag gtttataagt ctcgtctaga aacggctgat tggttagcac cagccacacg
     1141 tgaaaaagcc attactaaat taaatgttat cacgccacat attggttacc cagaaaaatt
     1201 accagaaacc tatgctaaaa aagtgattga tgagagcttg tcattggttg aaaatgcaca
     1261 gaacttggct aaaatcacga ttgctcacac ttggagtaaa tggaacaagc cagttgaccg
     1321 tagtgaatgg catatgccag cccacttagt caatgcttat tatgatcctc agcaaaatca
     1381 gattgtcttt cctgcggcaa tcttacaaga gccattttat tctttagatc agagttcttc
     1441 agccaattat ggaggaattg gtgcagtcat tgcgcatgag atttcacatg cctttgacac
     1501 caatggtgct tcctttgatg aacatggtag cctaaatgat tggtggacac aagaagacta
     1561 tgctgccttt aaagagcgca cagataagat tgtggcgcag tttgacggct tagagtcaca
     1621 tggtgctaag gtcaatggta aattgacagt ttcagaaaac gtcgctgatc taggtggggt
     1681 agcttgtgct ttagaagcgg cacaatcaga agaagacttc tcagcccgtg acttctttat
     1741 taactttgcg acgatttggc gtatgaaggc ccgtgaggaa tacatgcaaa tgctagctag
     1801 cattgatgtt catgctccgg gtgaactgcg aacaaatgtc accttaacca actttgatgc
     1861 tttccatgag acctttgata ttaaagaagg cgatgctatg tggagagctc cgaaagaccg
     1921 tgtgattatt tggtaattag gtagttgata aaccttcaaa tgcctgtgat tataaaaatg
     1981 actgattctg tgaaacacag gatcagtttt ttgcttcctt attggtgtcc gttttgacac
     2041 aacaaaaaga caaccctcag gaaagaaggt tgcctttttt agacttatca gaaataagtg
     2101 attagttgtt aagggcagca gccatagtag ctgcaacttc tgattcaaag tcgttagctt
     2161 ttttctcgat accttcacca acttcaaaac gtgcaaaagc aatcgcttta gcgtttactg
     2221 agtcaaggta agcttcaaca gttttgctgt cgtccatgat gtaaacttgt gcaagaagag
     2281 tgtaagcttg gtcaacttta gtgttgtcaa gcatgaagcg gtccatttta cctgggatga
     2341 ttttgtccca gatcttttct ggtttgcctt cagcagcaag ttcagctttg atgtctgctt
     2401 cagcagcagt aatcacatcg tctgataatt gagcttttga accgtatttc aagaatggaa
     2461 gagctggttt atcaaccatt gcacgagatt cgttgtcaag ttcgatagcg tggttcaatt
     2521 gagcaagttc atctttgatg aattgtgcat caagctcagt gtatgaaaga acagttggtt
     2581 tcattgcagc gatatgcatt gatacttgtt tagcaagagc atcgtcgccg ccttcgacaa
     2641 ctgagataac cccgatacgg ccaccattat gttggtaagc accgaagtgt tgctcatctg
     2701 ctttttcaat caaagcaaaa cgacggaatg agattttttc tccgatggta gcagttgcat
     2761 taacgtaagc ttcagcaaga gtttcacctg aaggcataac aagtgcaagt gcttcatcgt
     2821 tgttagctgg tttgccttca gcgataactt tagcagttgc gtttaccaat tcaacgaatt
     2881 gagcattttt agcaacaaag tctgtttcag cgttaacttc aacaacagca gcaacgttac
     2941 catgaacgta aacgcctgtt aaaccttcag cggcaacacg gtcagctttt ttagctgctt
     3001 tagccattcc tttttcacga agaagttcaa cggctttgtc catgtcacca tctgtttcaa
     3061 caagtgcttt tttagcgtcc ataacgccag caccagattt ttcacgtaat tcttttacaa
     3121 gcttagctgt aatttctgcc atgtgtagta tcctccaaat gatatttgtt ttggtttcaa
     3181 aaaacgagac agagcggttt gctttctgcc ccgtttggtt ttcgattgtt aaagacaaac
     3241 aaagtttgct ataagagtta ggaactctgt gctaattaag cgttgtcgcc ttcgacaact
     3301 tcaacgattt cttcgattga atctgcttga gtgtcagctt caaaagcaac gtctgcatct
     3361 tcaccttgac ggccttcgat aatagcgtca gctaatttag cagtgattaa tttaacggcg
     3421 cggatagcgt catcgttagc tggaatgatg atatcgatat catctggatc agcgtttgta
     3481 tcaaccatcg ctacgactgg gataccaagt tttttagctt ctttaacagc gatttgttct
     3541 ttatgtgggt caacaacgta cattacgtct gggatacgag gcatatcttc gataccgccc
     3601 aagaattttt caagacgagc gcgttgtttg ttaagaagtg caacttcttt cttaggaaga
     3661 acgtcaaagg ttccttcttc ttccatacgt ttgatttctt tcaaacgagc gatacgtttt
     3721 tggatagttc cccagttggt aagcgttcca cccaaccaac ggtggttgat gaagtattgg
     3781 cctgcacgcg ttgcttcgtc agcaactgct tcagcagctt gttttttagt accaacgaac
     3841 aagatgacag catcgtttgc tgcagcatca cgaacaaatt cgtaagcttg gtcagctaat
     3901 tttacagttt gttgaagatc gataacgtgg ataccgttac gctctgtgaa gatgtattta
     3961 gccatcttag ggttccagcg acgagtttgg tgaccaaagt gaacaccagc ctcaagaagt
     4021 tgtttcattg aaattactgc catgagtagt ttctccttta taaaatgttt tttcctcttc
     4081 cagacttcga cttgcaggct aacctatcgg caacaagccc acaattggtc tagaatgagt
     4141 atttgccgtc tacacgacct tactatagta tcaaaaaaag aggttaaaag caaggagaaa
     4201 tgctatggat tcccttattt ttgagatgac tagcttcttt gtttattggt aagtgttaag
     4261 acaagctttt gctcattttg ggtgacactt gccaaatcgt tttatttaag atatagtgaa
     4321 tttgactaat ttttggaggc gttttcatga cgaagggtat tcgatttcaa ttgcttggca
     4381 gtccccacat ttttcttgat gacaaagaac aattttttgc ctttgccaaa gcaaatgctc
     4441 tcctttatta cttagtggtt aatggatctg ttagccgcga ggtggcggcg agtctacttt
     4501 gggaaaataa gaacacgcaa acagcgaaaa agaatttacg aaacgctatc taccaggtca
     4561 ataaagtctt gcaagctgat gtgattattt gtcccaatcg caacctgctg gttttgaata
     4621 aaacacttga tatcaaaaca gatattaacc tttttctggc tgatccgcta gctcacttag
     4681 aactctacca aggggagttt ttgcagggct tttaccttaa aagtggtgag gaatttgatc
     4741 tttgggtttc aaaaatgcgt atgcagtatg aacaggtata cctaaaagcg tgttaccaaa
     4801 aaattgaaga aaagctctct ttagatgcca ttgaagatgt cgaggaacat ttaaaacagc
     4861 ttattgaacg tgatgaattt gaagagaaaa attatcagct cttgatgagg ctttatcagc
     4921 aggggaattg tcctggtaag gtcatcgaaa cttattatca gttggctaat gtgttggata
     4981 aggagctagg gattcagcca agtttgcaaa gccaacagat ttatcaggaa gtggtcgcta
     5041 aagatcgtaa tgaacgcaaa atcaagcatt ttttgcgcaa cagtaatcat tttttggggc
     5101 ggattgatga gattaagcag ctggaaaact attttgccaa ctgtttggcc tgccaagagg
     5161 taggagctct gctcttaatt ggtgatacag ggattgggaa acgaacccta gccagacagg
     5221 tcttagccaa tcaaacccaa acgtttcaaa ttgtcacagc taaatgtttt cgtgaagagg
     5281 ctatggattc cttattgcct tggcgtaata tcttagatgg cctgggagat ttggtgattc
     5341 aaaaccgctt attgaccacc aaagcttgga aggctgctct taaacgctgt tttcctgtgg
     5401 caaccatttt tcaagaagat aacaaccaac cctttatcaa ggaccacacc agcttgttgg
     5461 tttcctttat tgttgatatt ttacaacatt tggcagaaat caaggcgctg gtaatcctta
     5521 ttgaggattg tcactggatg gatgaggata gtttgacctt gttgcaacgg gtcatgaatc
     5581 aattggtcca ctatccgatt gcttttgtct tgaccaagca tctaggaacg actcctgagc
     5641 tcggtctttg tttgaatgct ttgatgagtc aagggcgttt agaaagcatt tgtttggagc
     5701 cttttaatcg gcaagaaagt ttggtttata ttaacagtca actgggtagt cagccagtga
     5761 cggcagaaga aatggagcac ctctatcaag ccagccaagg caaccctttc tttttgtcag
     5821 aatacactca ggccctactg cgtcacgaaa aatttgtgcc tttaacgcct gccattaagg
     5881 ctaagttggg tcttaaacta gctaatctaa gtagtcgtga tgacgcattg ttaaactatt
     5941 tgtcttgttg tcggaggcct atccctctaa ataccctcgc tcaattgatg ttactgcctt
     6001 tagaagaagt gatcgagatg gtggataatc tggggcacta ctatattttg gtagaagaaa
     6061 gcgtaggaga ggaagtgttg atttcatttc gtcagcggat tattcaactc tatagttatg
     6121 accgtttatc tttgtcaaaa aggcgtttat tgcacggaca aattgccaaa cggttagaag
     6181 atttactgcc tattttgaca ccaagccctc acttattaga tgacattgct taccattatc
     6241 aggaatcacg gcaggtgatt aaggctttgg aatacaacct taactatcta gatgctacct
     6301 tgccatttca gcatgagctg tttcccattt attcaaagag tattggctcc ttggaaaagt
     6361 cagatcgtga ccatcaacgc ttaatggaag agcagtttga taagattcgt caaagcattg
     6421 cggatttgga actgacctat gataataacc gtgatttcca gcagttgctt attcgttttt
     6481 cttaccttga aggccgttat gatattcgaa cagggaggta tcaagaaggg attaaacaca
     6541 ttcaaaaggt catcgctttg gcaaccgagc taaaacagcc ctccttcttg ctagaaggct
     6601 atcgccagtt aattcattac tgtatccaag tggaaaacaa gccagaaatg cgttattaca
     6661 ctagtctttc attggaggca gcggttgctg ctaatcattt tgaggccatt gcgattagtt
     6721 tgcggttaaa cggtctctat catttgatta ttggggagtt aaatgaggca gagcgtctgc
     6781 ttcaacagtc gattgacttc ttcaaggtga cgccaggctt gcaagctaac tatgcgattc
     6841 agattgctgc tgccttagat tatttggggg agattgccca gattcgctat cagtttgaaa
     6901 aggccgttgc ttatcaaaaa caagccattg ccttgaccga aaacaagcca gctgagttat
     6961 cggcgagtat tttttatatt ggattaggga tttcctactt ttatttagct gattttgaac
     7021 aagctgagca aatcttgagt ttggctaagg aagctttggt caatcatagt tatccttgga
     7081 aagaaacgca acttgaaatt tacctggcta tgattcaatg gaaaaagggc aattatcagc
     7141 cagccttgac cctacttgat tacagagaaa ccttgatgtc taggtatcgc aatccgcgtg
     7201 ataagggctt ggttttctat ttgatggctg tggtcaagta ccaattgata tttcaaggag
     7261 ctaccctaag tcagcaggaa aaagaaatgg cagaccacct gttgtcagaa tcctttgagt
     7321 attattatga gattgctagt actaacctca acccttaccg agattgtcat ttggtgagtg
     7381 agttgaacga tttgcgtcaa caattatctg ctaaaagcta aggaaaaccc tctgaagtga
     7441 atcagagggt tttagctatg ttgtgccata atctgtacga ggtaaaagat aaaggtggct
     7501 gctaaattgg cggtgtggtt atcaatatcg tgtggtggtg aaacctcaac cacatcaaag
     7561 ccaactaatt ttccgctagc tgcaatgtgt tgcaaaacta agacggcaag atttgggtca
     7621 acaccgaggg actggatagc actaacccca ggcgcagcac caactgagaa acaatccatg
     7681 tcaatggtca ggtagaccct ttcttgccct tctaagaaac ggtcaatggc tcggcagacc
     7741 ttttgatggc ccatttggta gatgtcttga cctgttaaaa attgaatgcc cttggatttt
     7801 gccacaaagt caaataggaa aaggttgtta ttatgttctt gaatgcctaa aacgaagtat
     7861 ttaaagaggc gtttgtcagc aaccgcatcg tcaaacatct gtcgaaagcc tgtccctgaa
     7921 ttagggcctg tttggtcgta aggccttaaa tcaaagtggg cgtccatatt gatgacagct
     7981 aggtcatcac ttggggatag gctttggcgt aggcctaagt agtggccgta ggcggtttca
     8041 tggccacctc ctaagacgat gggtttgaga ttgaggtcac acatccgttt gatggctttg
     8101 cttaaactgt tttgcagttg ttccaaagag cgattggggc cgtcaatgtt acccacatcg
     8161 taaaccataa cttggtttcc taaatgccaa gggaatttgg cgagctgggt tcgtatggca
     8221 gctggacttt cgaccgctcc gacacgacca ttattgatat aaaccccctt atcactttta
     8281 aagccaatca aggcaaaatg tgtcccttca aatggggtta gtgagctgtc gttaaggtca
     8341 aggaaagtca tgaccatgcc ccatttggca gtatataagt catcgtcgat cccaccatga
     8401 taatagctag tggtggatgg gtagtaatct tctagcatgc gcgtcatctc ctttaggatt
     8461 atgatagcaa aataaaactt acagtcaaca ctttcttggc ctttgtcact ggtttttagg
     8521 aatagcagag caataaaatc gtctgctttt ggtcccggtt agttagtcta aagaggccat
     8581 gacctctgcc acagcccctc tagttaaaga agtaccttat tcactaaaat tggatactca
     8641 actcaactgc ctgctcaacc gcttcgagga agcttgggtc ttcaatgact gctgatgcct
     8701 tgtttagttc atcgtagatt tcgatgtgtt tatcgtgttc gataaagttg acttccttgc
     8761 ggaagagatc gtaggcaacc ttagtccctt tacctagttc atgattttca ggtttgagat
     8821 caagggcttg gcaggctgcc atgatttcag tagccacgat gcggcgagaa tttttaagga
     8881 tttcaaaggc cttgcgagca gcggtggtgc ccatgctgac aaagtcttct tggttctcac
     8941 aagatgggat agaatccaca ctggctggat gtgccaaaac cttgttttca gaagcaagag
     9001 aggcacaggc gtactgggta atcataaagc ctgaattgag acctgggtat ttgacaagga
     9061 aggatggcaa tttgctgagc tggctattga ccaaacgttc cacgcggcgt tcagaaacat
     9121 ttccaatttc agaaatggcg atgcctaaga agtcaaatgg ctgtgccatt ggttctccgt
     9181 ggaagttacc tcctgaaata acgtggccat ctttacagat aattggatta tctgtgacag
     9241 aattgatttc aatgtcaact ttggatttga cataggcgat actgtctttg ctagccccat
     9301 gaatttgagg catgcagcgc aaggtgtaag ggtcttgcac acgagattga gtagctacgg
     9361 ttgtgttttg gctgccttca aggaggtttc ggatattgcg ggctgttgcc aactggccgc
     9421 tttgtgggcg aatggtgtga aggttttctt caaatggact agtaatcccg ttatgcactt
     9481 caagggatag ggcaccagct aggtctgaca gtttcaataa ttgaatggcg tcataggttg
     9541 caagggcacc gactgcagtg agaacagttg ttccgttgat aagggcgagt ccttctttag
     9601 cggctaaaga aatcttgtca ataccagctt tatcaagagc ttcttgacca gaaagaagct
     9661 ctcctttgta gtaagctttc cctagaccaa gcataggaag caccatgtga gcaagcggcg
     9721 ccaaatctcc tgaagcccct agagaccctt tttcaggaat gtaaggatga acccctttat
     9781 tgagcagttc caacagtttt tcaatggtgg atagacgaat accagaatac cctttgacta
     9841 gtgaattgat acggatcagc atgatagctc tcacggcatc ttctggcaat gggtcaccaa
     9901 atccactagc atgggtacga atcaaatttt cttggagttg aactgtatct tctggtgaga
     9961 tgctcacgtt acaaagggat ccaaaaccgg tggtcacccc gtaaaccacc cgtttttcac
    10021 tgacaatgtc atcaacaatt ttacgagagg cattcacagc ttcaatagca ctatcatcaa
    10081 tgtgacaggc tacgccttgg cgagcaatcg caatcacgtc ttcgatagta aggctttcgc
    10141 catctaaatt aatcactctt gtcatagttt ttctcctaga tttgttcctt agggggcatt
    10201 tggtccattt ggtggttacg tccgtgcata aggtagtaca cactacttgc aatgatgaca
    10261 ccaactgcac cataaacaaa gttcattggg ttgtcacccc aaatcataac taaactaacg
    10321 ataactgcaa gaattgggat aatagggcca aatggtactc ggaagatcac attagcatcg
    10381 ggatcgtcct tccgtaattt cataacggct agtgctgttg gaatgtattg gaagaagcgg
    10441 aaaacaacac tgagttttgc taagctttca aatgagccag taagcaggag tacaatggca
    10501 attgctcctg aaaccaagat agcaacgagt ggcgcgccat tttgattttg cttggcaata
    10561 gcagctggta agagcccttc atcagcaatg gcagcaccgt aacgtggcac catgatggat
    10621 tctcccatgt taagacctgt aatggaaatt aaagcaccga ttgagaccat ccaagcgcca
    10681 gcagggccta tcattttaac aaaggcatct tgaacaggtg cattggtcat catgatttga
    10741 gagccaagca tggcaatggt tccaccgata atcagcatat ataagacaga gacgatactg
    10801 attgatccta aaagagcacg cggtacattt ttttcagggt cacgcatttc cccagcgaca
    10861 atagagaggg tttcaaaacc gataaagcca tagaagatat aaacagcagt attggagata
    10921 gcccctaaga ggttagtccc tggttccaac tgaacaaatg gggtaaagtt aggaagcccg
    10981 tttttgatga agaaaagggt acaggcacaa aaggcaacga ttggaattaa cttagcaatg
    11041 gtggctgtga tggtgacaat tttagaggtt ttcaaaccag cgatattcat caaagatagt
    11101 aagatgatta acccaatact aagtggaatg tgccaacctt caaaggcagg gaaagtaatg
    11161 ataaacattc tggcaaaccc agctgccatg gctgcccaag cgaagatggt aacggtccaa
    11221 ccaaggaagc caacgttaaa gccaataaaa tcaccaaaag ctcgtttgga atattggaag
    11281 gctccgccgt ttttcccaaa ataaccagag acttctgcaa aacaaactgc caacatgatg
    11341 gtcaaaatag ctgttccaaa cataacagcg atggatgcag ggccaaggcc cttataaatg
    11401 gccctaggta agagaaaaat accagagccg ataacggcat tgatcccgta aagagtggca
    11461 ccgctcaagc taaactttgc ctgttctctt tcttgttcgt tcatgtgatt tgacgccata
    11521 ataatgctct cctttttgtt tgcaaattgc gttcaaatat aatggataag gtcgctagga
    11581 aatgaggaga tagtcggttt aggaggatcc ttcttgcacc taacgatagt gatgagctat
    11641 tggttagcga tgagaaatag gtagcctttt gtaatgatgt gtagtgattt cattggagaa
    11701 gaataggcat gagtagtgtg atgcctatct ttatcccact aagtcttata ccataagcct
    11761 gcccaaacag ctactggagt ttgggcgggg cttgtttgat tgaggcaaac ttttagatct
    11821 gaaaggctgg cctcctgctc ggttaccaac aagtgattgg cttcaagctc ttgattcgcc
    11881 tgattggtca cagaataagt cagtttgctt agggcataaa gcagttgaca gtccgcttta
    11941 ggtgcctcaa atggtgtgtc agaccagacg ggtaataatt gcccttggta ttggtcatca
    12001 tacattaaat tgatgtcttg gcaggttcca cgactgacga ttctggtatc gccttcaaac
    12061 gcatagggtt gaaaaggagc tagggaaatc tctttagcat gactcaaatc acttaatgtg
    12121 atgggcttgt ctaggctcat tagaatcctg tgatagccat caagtcgggt aaaggttgtc
    12181 tgtcgtaggg ctacagttgc agttgagaga cgataagaga attctctctt tttgtaatct
    12241 ccatcttcgg gataaaggaa caattggttg gtttcaccgc cagaccagtc agtttggagg
    12301 taatcagagg gcgctttgat ggttaccttt gtcatttgtt ctctccttat ttccaattag
    12361 aatagtcctg tgatgttgcc ctcctcgtca atatcaattg tttcgctggc aggtactttt
    12421 ggaaggccag gcatggtcat gatagcacct gttagggcaa cgataaatcc tgctcctgcg
    12481 gacactttaa ggttgctaat agtcactgta aagtcagttg gtgcgccaag ttttttggca
    12541 tcgtctgaga aggaatattg ggttttggcc atgcaaattg ggtagttgcc aaagccgagg
    12601 cgttctaagt cagccagttc acgtttggct gctgagctca agttgatgcc tttaccaccg
    12661 taaactttgg taacgatttt agtcagtttg gtttcaatgc tatcgtcttc ctcataaaca
    12721 aagcggaatt gattgtcttg ctcggctagt gtcacgactt tttcagccag ttcacggcca
    12781 ccagctccgc catttgccca aacatcagaa atcacaacgt caacgccacg tttgtcgcag
    12841 gcatcataga cagcttgtaa ttcggcatcg gtatcaagcg ggaatttatt gatcgccacg
    12901 acaactggaa ggccatagac gtcttggata ttagccaaat gtttgtcaag gttaggtaaa
    12961 ccatccacaa cggcctgaac attttcagta gccaggtctg cttttggcac acccccatgc
    13021 attttaaggg cacgtatagt ggctactagg acaacggctg ctgggcgaag gcctgacatg
    13081 cggcatttga tgtcaatgaa tttttcagct ccaaggtcag cgccaaatcc agcttcggta
    13141 acggcataat caccatattt caaggctagt ttggtggcga ggacactatt acagccatgg
    13201 gcgatattag caaatgggcc accgtgaatg agggctggtg tgtgttccag ggtttgcacg
    13261 agattaggat gaatcgcatc cttgagcaga gcggctaggg caccaccagc tttcaagtct
    13321 ttagctgtta caggttcacc ttggtagttg taaccgatga tgattttttc aaggcgggct
    13381 ttgaggtcag aaatattttc ggataggcac aggattgcca tgatttcaga ggcgacggta
    13441 atgtcataac catcttcacg aggaatcccg ttgaccttgc cttgcaagcc gtcaacaata
    13501 tgacgaagct ggcggtcatt catatccacc acacgtttcc aagtgatacg tctggaatca
    13561 atgcctaggc tattgccatg gtgaatgtgg ttatcaatca aggcagctaa taagttgttg
    13621 gcgacaccga tggcatgaaa gtcccctgta aagtgaaggt tgatgtcttc cattgggaca
    13681 acttgcgcgt ggccaccgcc agcagctcct cccttaaccc caaaaacagg accaagagaa
    13741 ggttcacgaa gggcaataac tgcttttttt ccaatggcag aaagagcatc taccaatcca
    13801 acagaagtgg tggttttgcc ttcaccagct ggtgttggtg agatagcagt gacaagaatg
    13861 agtttgccat caggtttgtt ttttaaggcg accagttgac gggcatcaat ttttgccttg
    13921 tacttaccat agagacagag cgcctccttg tcaatcccta attgatcggc aaccttactg
    13981 ataggttcca tagtgacaga gttggctatt tcaatatctg ataaaaccat agttgctcct
    14041 ttacttgtta aacaatatcg agaattttcg tgtagatgtc atctgccaaa tgacagccct
    14101 tgtctagtaa ggcttgtcct ttttggcgat agtcggtaac aaagtcttca tctttgatgc
    14161 cagacagatt aatcaaaaca ttcaaccaag cgccttgtaa acctgctttt aagtttaaag
    14221 cagcaactcc taagtcgcta gcagcatttg tattagactt accgacagct gttgcagtga
    14281 tctcaagtgc ttcgaccatg agggtcatca tctcaaaagg tgattgtgca gctgttttca
    14341 aggccttttg catggcagtt cgtctagcag ccttgtcttc gtcagtctct tttggcatat
    14401 caaaaacggc agaaaccagg ttgaaggctt cggtatcttt gtcaatggct gcaagcaagc
    14461 tagcttgtag agcggtgctt ttagcatgga tctctgtaat aatatcttgg tagtcagcgt
    14521 attttttctt accaagagtc aattcacaga ccatcttggt taaggaaatg ccattagcgc
    14581 cagagagagc agcagcagaa ccaccacctg gagcaggggc atctgagcca aggactttag
    14641 cgaaatctgt taagcttaaa tcaactaaac ccatagagaa cctcctaacc tagcaaatgg
    14701 ttttcaagaa tctgtttatg gtagtcaaag tcctcaactt gtaagtagta ttcagccaca
    14761 tcgattaagg ccttggctgg cgctaagccg atgacttcag aaccaataac attcacaccg
    14821 tagcgacgcg cttcaaattt gatggtttca aaagtacggt aaagagaaca tttttcaaag
    14881 ttaaccatgt tcatggaaac ttgggcaata tgacggtctt ctaacatgac accgatagcc
    14941 ttacaatatt tgtagccacc acctgaaccg cggataattt tcgcaatttt atgagcaatg
    15001 tcgatattgt cagtatccaa attaacattg aaagcaacca atggcatgcg agcaccaacg
    15061 gcagttaccc cagcagttgg gtgaatctta cgatctccgt agtcaggtgc ccaatcctct
    15121 tccaacagtt tttctggcat gccttcgaat tgtcctttac ggactttggc caggttttga
    15181 cgttctgggc gtgtggcaga atcttcataa aggaagattg ggattccaag ttcacggtta
    15241 atccgttctg cgacttgttt ggaaatctca acacattctt gtgtagtgat gtctttgatc
    15301 ggaacgaatg ggcagacatc ggttgctccc atacgagggt gttcgccatg atgtttggtc
    15361 atatcaatgt tttcagaagc gtatttcacc agttggaaag ctgtttcttg aatagactgg
    15421 tcatctccaa ccaaggtaaa cacgctccgg ttgtggctcg catcagaaga gtagtcgaga
    15481 agggtcactc caggaatact tttggccgtc gctaccaaac catcgataac agcctgattt
    15541 tggccttctg agaagttggg aatacattca acaattttcg ccatgtttaa tctcctttta
    15601 tgtcattgaa ttactcatta agggaatagg ttatctactt tagataacgc ttacattttt
    15661 agctagcagt tattagtgaa ataattgatc tactgctgaa gtcacaaggt catcatcagc
    15721 caagtatggg atagtgatgt gatcagtacc tgcatggaga cgattatact caatagcggt
    15781 ttcaatagcg tgctcattgc gagcccaatt gcgacgagca acaccaccca tggtgtccca
    15841 agcaatagca gatttaataa tctcatcaat gcgttcgctg ccatctaaaa caagtccgaa
    15901 accaccattg atggctttac caataccagt tccgccaccg ttatgaagag ccactaaact
    15961 cataccacgc gcagcgttac cagcgtaaca ttgcaccgcc atatcacagg tcacattgga
    16021 accgtcttta atgtttgagg tttcacgaaa tggtgagtcg gtaccagaca cgtcatggtg
    16081 gtcacgacca atcatgacag gaccaatctt gcctttgcgg accaattcat taaatttaag
    16141 ggcgatagtc acacgaccga tacagtcttg gtaaaggatc cgggcttggg taccaactac
    16201 gagttgattt ttctcagcat cacgaatcca gttgtagtta tcacggtctt gatagcggcg
    16261 gtcaggatcg atagcttcca tggcagcttt gtcagtggcc accaagtcat cgtgattacc
    16321 actcaagcag acccaacgga aaggtccata gccatagtca aaaagcatag gtcccatgat
    16381 atcttccaca taggatggcc agataaatcc gtctttgtcg ttacgaccat tcttagaaat
    16441 ttctgtaata ccagagtcgt aaacagactt cataaaggca ttgccgtagt caaagaaata
    16501 ggtgccattt tcagttaacg tcttaatagc ttcaaaatgc cgtgccaagg tatcatcaac
    16561 catttggtgg aaggtatctt tatcttctgc caagagccgg gttctttcgt caaagctaat
    16621 acctactggg cagtagccac cgtcataaac gttgtgacaa gaggtttggt cagacaaaag
    16681 atcaacatga atctgcttgt cattgacgta ctctaaaaga tcaacgatat taccatggta
    16741 agcaatcgaa gtggactcct tagcatctat ggctttttgg gctaattgca gagcttcttc
    16801 agggctttcg gcaatttgac tgatccagcc ttgagaatga cgggttttaa tccgagattg
    16861 gtcaacttca gcaataatgg caactgcttt agcaatttca gcagccttac cttgtgcccc
    16921 actcatgcct cctagaccag atgagatgaa gagtttaccg gtcaaatcac cgtcatcagc
    16981 cactccaagt ttgagacgac cagcatttaa gagggtatta aacgttccat gaacgattcc
    17041 ttgtgggcca atatacatcc aaccacctgc ggtcatttga ccatagttgg tgacacccat
    17101 ctcttcagcg atttcccagt ctttcatgtt gtcgtattca cccaccaaaa ggccattagt
    17161 aataatcaca cgaggcgctt caggtttgga tttgaaaaga ccaactggat gaccagactc
    17221 aaccaccaag gtttgttcat cagtcatgac ttctaggtat tttttaatca aacaatattg
    17281 catccagttg gcacaaacag aaccagtttc tccatatgtg actagttcgt aagggtacaa
    17341 ggcaatttca aaactcaagt tattgtcaat catgacctgc atggccttgg cagctgtaca
    17401 atttcccttg tactcgtcaa tgggtttgcc gtaaatgcgg tcttttggtc taaagcggta
    17461 gccataaata cggccacggg ttttgagttc ttccaaaaat tctggaatga cttcttcgtg
    17521 gaacttcgta ggtacatagc gtagggcatt tttcaaggcg atttcggtct gagcctgggt
    17581 caatcgaaaa cctctgtctg gtgctcttcg gataccttct tcaaacacag tcttttccgg
    17641 taaaacatcg tctaacttaa cagtcatagc ggcagctatg tcagtttcac tgtaaaatga
    17701 catagaaaac tcctcctcaa ctccttttta ttgtcgagat tagtctaact tagtctagtc
    17761 tataaaaagt caaccacgct ttgaaatgcg aaaaaagtct aaaaaaaaat caaaacctca
    17821 ttttttgact aaaatttgac gcttaatcag tacacttgag gtatgacaga taaagtcagt
    17881 cagaggttaa ttgttgttac caaagttgca aagatcgcaa ctattgagtg ggtgaggggg
    17941 gactagtaat ggctattaat cagttgacaa acgatgttaa aaaaggaggt ttttatggtt
    18001 gctgatgtct tattaacaca ttttaatcaa ctattttgtc taaatgatcc aggtcatcct
    18061 ttaactggtc aagaaatgaa aaaagccact atcgttgaag atggctatat agccatcaaa
    18121 gacggcctca ttgtggctct tggttctggt gaaccagatg ctgagcttgt cggtacacag
    18181 actattatgc gttcttataa aggtaaaatt gcaacgcctg gtattattga ctgtcatacc
    18241 catttggtct atggtggcag tcgtgaacat gaatttgcca agaaattagc aggggtgtct
    18301 tacttggata ttttagctca aggtggtggt attttaagca cggtgagggc cactcggtca
    18361 gccagctttg acaaccttta tcaaaaatcc aagagattgc tagactacat gttgcttcat
    18421 ggagtgacta ctgtcgaagc gaaaagtggc tacggccttg attgggaaac agaaaaacgc
    18481 caactggatg ttgttgctgc tttagaaaaa gaccatccca ttgatttggt gtctacgttt
    18541 atggcagccc atgcgattcc tgaagaatac aaagggaatc ctaaggctta tctcgatgtg
    18601 attatcaagg atatgcttcc tgttgttaaa gaggaaaatc tggcagaatt ttgtgatatt
    18661 ttctgtgaaa aaaatgtttt tacagctgat gagtctcgct atctgttaag caaagccaaa
    18721 gaaatgggct tcaagcttcg tattcacgca gatgaaattg cgtctatagg cggagttgat
    18781 gtcgcagcag agctcagcgc agttagtgca gagcatttga tgatgataac agatgatggt
    18841 atcgctaagt tgattggtgc tggtgttatt ggtaatttgc ttccagccac cacctttagt
    18901 ttgatggaag acacttatgc ccctgctcgc aaaatgatcg atgctggcat ggccatcacc
    18961 ctctcaacag atagtaatcc aggcagttgc ccgactgcta acatgcagtt tgtgatgcaa
    19021 ttaggttgtt ttatgttacg tttaacaccg attgaagtct taaatgctgt taccattaac
    19081 gcggcctact ctgtcaatcg tcaggagaga gttggtagtt tgacagttgg taaagaagca
    19141 gatattgcca tttttgatgc cccaaatatt gattacccat tttatttctt tgccaccaac
    19201 ttgattcacc aagtctataa aaaaggacaa ctcacagttg accgaggtcg tattctttaa
    19261 aaaatttaaa aaagaagctt tgagcgttag ctcaaaactt cttttaaata tgctgtttta
    19321 ttgacgaatc aagtagtcaa aggcaccgat ggcagcagta gcaccagacc ccatggaaat
    19381 aatgatttgt ttataagctg aatcagtaca gtccccagca gcaaagatac caggaatatt
    19441 ggttgaccca tgtttatcca caatgatttc tccgcggtcg gttaggttga caccactatc
    19501 tttgagccaa gctgtgtttg gaacaagacc aatttgaaca aagacccctt caaggtcaag
    19561 gtgtttgtct tcaccgctat cacgctcagt gtagttaaga cctgtaacat ggtcctctcc
    19621 aacaatgtcc ttagtagcaa catttttgat gatggtcata ttgttagtct tagctgcgcg
    19681 gtcttgaagc actttgtcag ctttaagttc aggcaagaat tctaaaacgt aaacgtgttt
    19741 agcaagacca gccaagtcaa gggccgcttc taatccagag ttcccaccac cgataacagc
    19801 aacatctttg ccttcaaaga gaggaccgtc acagtgaggg cagtaagtca cccctttatt
    19861 acggaattca tcttcaccag gaacattaat gttacgccat ttggccccaa gagctaagat
    19921 agcagttttg gcttgtagaa ccgcaccatt agctagagta acctcgatgt tttctttttt
    19981 ctcaatagaa gtagctagtt gtgctttgat aatatcaaca tcataagact tagtatgggc
    20041 ctccacttcg gccattaatt ttggtccttc agtgtaaagg gtaccaatca tgttttcgat
    20101 gccgaccgtt tccatgacct gaccaccaaa ggtttcagca agtaaacctg tttttagtcc
    20161 tttacgagct gcatagatag cagcgctatt gcccgcaggg ccaccaccaa taacaaggac
    20221 atcatagagg cctttatcag caaaggcttc ctcagaaagt gggccagcga tttgttctaa
    20281 gagttgctca atggtagcgc gaccagaagt gaattcttcc ccgtctaaaa agacagtagg
    20341 gacagacata atgccttttg ctttcacttc atcttggaac atgccgcctt ctaccatagt
    20401 gtgtgaaatc ttgtcgttca agacggacat aatgtttaaa gcttgaacaa catctgggca
    20461 gttatggcag gttaagctga cataagtttc aaaatgcaac ggacggtcaa tggccttgat
    20521 gcgatcaata acatcttgat ccacttttgg agcacgtcca gatacttgca agagagcaag
    20581 aataaaggac gtcaattcgt ggcctaaagg aagaccagca aagacaaccc cactaccgtg
    20641 accttttttc gcaaccttaa agcttggttg tcggtctaaa gtgatgtttt caatagaaat
    20701 acgctcagac atagcagcta tttcttccac aaagtctttg actttttgag actgctcatt
    20761 gtcaccaagg gaaacttgca aaacaagatc agcttctaac agggtgaggt attgggcaag
    20821 ttgttcttta atatcaggac ttaatgccat agctttcctc ctaaaattta ttaaatttta
    20881 ccaactaaat caagacttgg tgtcaaagtt tcagcgcctt ctttccattt agctggacaa
    20941 acttcacctg gatgtttacg gacgtattgg gcagcgtgaa ttttatcaat caaggtgcta
    21001 gcgtcacgtc caataccatc agcattaatt tccatcattt ggataatacc atctggatca
    21061 acgataaatg ttccacgttg agcaagtccg tcttcgccaa gcacttcaaa ggcttgtgaa
    21121 ataaggtgtg aagggtcacc aatcataggg tatgtgatag tgccaaccac atctgaatca
    21181 tcatgccaag ctttatgaac aaaatgagta tcagtagaga cagaataaac ttctacacca
    21241 agagatttca gtgtttcgta ttgctcttga aggtcaccga gttcagttgg gcaaacaaat
    21301 gaaaagtctg ctgggtagaa acaaaaaact gcccattttc ctttaacgtc ttcatttgta
    21361 acagtgatga attttccatc gtgataagct tgagctgaaa attcagcaat ttcttttcca
    21421 attagagaca tatagttgtc ctcctttttt atagttttat acttgtatta taaaagaaca
    21481 acaagattat gtctactcag aaacccttga ttttactggg aaatcagcga ttattttctg
    21541 acaattaagt tgtgaaataa atcacattaa ttaaaatgat tctaaataag ctgaagatgc
    21601 cgttaaatca aaggttaatt taattataat ctacagaaaa tataattgtt attaaagcgt
    21661 ttacaaaaaa cttttttgat tccgttaagg caacttaata ttatggttac ctaatcgcta
    21721 atattgtaac ctattttggc atgagatcag ggaatggctg ttatattaat tggatatgtc
    21781 ttgtgcaaat agtgcttgac ggatttgtgg aattagggta aaataatctt tgttgtggcg
    21841 gtatagccaa gtggtaaggc acggctctgc aaaagcttga tcgtcggttc aaatccgtct
    21901 accgcctttt atatacaaaa caagccttga ttaacttcgt gaaaacgttg ttaaatcaag
    21961 gtttttattt tttgcggcag gataaaaaaa gagaaaaaaa gaaataaagt cggtcaaaag
    22021 tcggtcaaat ttcccgaaga tgtcttttag agcctgatta ttaggtatga aaaatactat
    22081 ggccaaaatt ggtaaacgat tgcaaataaa ggtcagagtt cttaccccct taaacgatat
    22141 gcgttaaaag ttagttgaac tgccgtgcac cttaacgata gacacggtga tgtgagagcg
    22201 agtagagttg aatctctact tgtttacctt accatagtca gctttataaa gtaaccaccc
    22261 attacaatgc aactatattc ttaatgtttt taagcatgtt attgacaagt gttataaaat
    22321 gtgttactct tagtgagtaa attagttttt gagttagtat ttgtgtttca acatctttat
    22381 ttctctctat ttttacaaat gatttttaga aaggaagtgg atgtatatgg cacaaggtac
    22441 agtaaaatgg tttaatgctg aaaaaggttt tggttttatt tcaactgaaa acggtcaaga
    22501 tgtctttgca catttttcag ccatccaaac taatggtttt aaaacgttag aagaaggaca
    22561 aaaagtggca tttgacgtcg aagaaggtca acgtggacct caagcagtca atatcactaa
    22621 attggcctaa tctggttaaa gtaactaaag agaagcagat gttggaaaca gcaactgctt
    22681 ttttgatttc taccagttac ctttaatgat taagagttgc gtttaaggtc ttgtatttgt
    22741 tataataata gtcagtttta gtcaaaatgt attgacgtgg tgctactaag tttttagagt
    22801 agcccgcaag caaaggagtg acagcatgcc aaccaaaaat acttcagaca gcattgaaga
    22861 gtatattaaa gaattattag ctaagtcagg aatagcagaa attaagcggt ccatgctagc
    22921 agattcgttt caagttgtcc caagtcagat caactatgtc attaaaaccc gttttacgga
    22981 gagtcgaggc tatgaagtag agagtaaacg aggtggtgga ggctatattc gtattgccaa
    23041 ggttcatttt tcagacaaac accatctgat tggcaattta atggcaacta ttgaagactg
    23101 tatcagtgag caagttttca cggactcgat acaattgctt tttgacgaac acttattgac
    23161 agagcgtgag ggaaatatca ttttggctgt ggcgtctgat gatgttttag gaactgatgg
    23221 ttcaactatt cgtgcacgaa tgctctatcg cttattgcaa cgaattgaca gaaagggaag
    23281 caattaatga ttatgtattc aacgaagatg caagacattt ttagacaggc gcagttccaa
    23341 gctgctcgct ttgatagcca ttgcctggaa acttggcatg ttttgttagc tatggtagct
    23401 gtagataatt ctttagcaaa tatgatttta agtgaatatg atgcccaagt cgccatagaa
    23461 gaatatgaag ctgcagctat tttagccatg ggcaaaaccc ctaaagaaca gttgtctcgt
    23521 gtagacttca gacctcaatc taaaactttg actaacttgt tagcttttgc gcaggctatt
    23581 agccaaatca ctagggatca agaagtcggc tctgagcatg tcttatttgc tattttattg
    23641 aatccagata ttatggcgag tcgtttgtta gaaatagctg gctatcagat aaaagataac
    23701 ggcaatgggc agccgcgatt agctgacttg cgaaaagcaa tagaacgtca tgcaggttac
    23761 agtaaggaaa tgatcaaggc tattcacgaa ctacgtaagc ctaaaaaaac gaaaacacaa
    23821 gggacctttt cagatatgat gaagccacca agtacagctg gtgagttgag tgatttcaca
    23881 agagatttga ctgaaatggc aagacaaggt ttgttagaat cggtgattgg acgtgaccaa
    23941 gaagtatctc gtatgattca ggtactaagt cgtaaaacga aaaacaatcc tgtcttggta
    24001 ggtgatgcag gtgttggtaa aactgcgctt gcttatggcc ttgctcaacg gattgcaaat
    24061 ggcgctattc cttatgaact taaggagatg cgtgtcctag aattagacat gatgagtgtg
    24121 gtagcaggaa cccgttttcg tggggatttt gaagagcgca tgaatcaaat cattgatgat
    24181 attgaagctg atggtcagat tattcttttt gttgatgaac tacatactat tatgggttct
    24241 ggcagtggta ttgacagtac acttgatgcg gctaacattt taaaaccagc attatcgcgc
    24301 ggcactcttc atatggttgg agcaacaact caagaagaat accaaaaaca tattgaaaaa
    24361 gatgcagctc tttcgcgtcg ttttgctaaa atattaattg aagaacctaa tacagaagat
    24421 gcttatcaga ttttgatggg cctaaaatta tcttatgaga cctaccataa tgtctcgata
    24481 tcaaatgagg cagttaaaac agctgtaaaa atggcacacc gttatttaac cagtaaaaat
    24541 ctccctgatt cagctatcga tttactagat gaagctagtg ctgctgtgca aaacatggtg
    24601 aaaaaatcag cacctgagac tttaacacca atagaccaag ctcttatcaa tggtgatatg
    24661 aaaaaagtat ctcgcctctt agctaaagaa gcaaaaggtc agatgagaaa accaacaccg
    24721 gtgacagaag atgatatttt ggcaaccttg agtaagttat cgggaattcc acttgaaaaa
    24781 ctgacgcaag ctgatagtaa aaaatacctc aatttagaaa aagaactgca taagcgtgtg
    24841 attggtcagg atgctgctgt tacggctatt tcaagagcca ttcgtcgtaa tcagtcaggt
    24901 attcgaacag gaaaacgtcc tattggatca tttatgtttc ttggcccaac aggagtaggt
    24961 aagacagaac tagcaaaggc ccttgcagaa gttctctttg atgatgaagc agcgcttatt
    25021 cgttttgata tgtctgagta catggaaaaa ttcgcagcgt ctaggcttaa tggagcacct
    25081 cctggttatg tcggctatga tgaaggaggt gaactgacac agaaagttag aaataaacct
    25141 tattcagtct tgctttttga tgaagtggaa aaagcacatc ctgatatttt taacgttctc
    25201 cttcaagtat tagatgatgg tatattgact gatagtcgtg ggcgtaaggt cgatttttca
    25261 aatactatta ttatcatgac cagcaatctt ggcgcaacag ccctgcgcga tgataaaacg
    25321 gtcggttttg gggtcaaaga cattcaccaa gaccatcaag ctatggagaa acgtatttta
    25381 gaagaattaa gaaaaactta ccgcccagaa tttatcaatc gtattgatga aaaagtggtc
    25441 tttcatagtc tgacccaaga taacatgcgc gatgtggtta aaatcatggt acagcccctg
    25501 attactacat tggcagaaaa aggtattacc cttaaaattc agcctttggc cttgaaacat
    25561 ttgtccgagg tcggctatga tgagcatatg ggggcaagac cattacgtcg aacgctgcaa
    25621 actgagatag aagataagct atcagagctt attctttctc gagaattgac aagtgggcat
    25681 acgctaaaaa ttggattatc acatggcaaa ttaacgtttc acatagctta atttccatat
    25741 aaaaggtgga tcatccttga tgtcaccaaa aaaagaggtt attcttacaa aaataacttc
    25801 ttttttcttg actatttttg accaagtgat aaaatagaat ataaagttag cactcgatga
    25861 acatgagtgc taactttact aaagattctt ttattggagg aaaatttcat gttaaaacca
    25921 ttaggtgacc gtgtggtcgt aagatttgat gatgaaaaag agcagacagt cggtggtttt
    25981 gtccttgcag gcactcataa agagtcaaca cgaaaagcga cagtcctcgc tgtcagtgaa
    26041 acgggtgttc gcactattac aggtgatagc gtgctacctt cagtttcagt gggtcaagag
    26101 gttttagttg aaaatgggca tgatttagaa gtaaccgtag atgatgagaa agtttctatc
    26161 atccgtgaat ctgacatcat tgctattgtt acaaaataaa acgacaaatc tctaaataga
    26221 aagaaggatt gaatatggca aaagatatta aattttcagc agatgcgcgt gctgccatgg
    26281 tgcgcggagt tgatatgtta gcagataccg tcaaagtaac gcttggtcct aaagggcgca
    26341 atgttgttct tgaaaaagct tttggttctc ccttaattac taatgacggg gtaaccattg
    26401 ctaaagagat cgaattagaa gatcattttg aaaacatggg agcaaaattg gtgtctgaag
    26461 tggcttctaa aaccaatgat attgctggtg atgggacgac tactgcaaca gttttgacac
    26521 aagccattgt tcatgaagga ctaaaaaatg tgacagcagg tgctaatcca attggtatcc
    26581 gtcgaggcat tgaaacagca acagcaacag ctgttgaagc cttgaaagcc attgctcaac
    26641 ctgtatctgg caaggaagct attgctcagg tcgctgcagt atcatcacgc tctgaaaaag
    26701 ttggagagta tatctcagaa gctatggagc gtgtgggcaa cgatggtgtg attaccatcg
    26761 aagaatctcg aggtatggaa acagaacttg aagtggttga aggcatgcaa tttgaccgtg
    26821 gttacctgtc tcaatacatg gtcacagaca atgaaaaaat ggttgcagac cttgaaaacc
    26881 catttatctt aatcacggat aaaaaagtgt caaacatcca agacattttg ccactacttg
    26941 aggaagttct taaaaccaac cgtccattac tcattattgc agatgatgtg gatggtgaag
    27001 cacttccaac ccttgtcttg aacaagattc gtggtacttt caatgtggtt gctgtcaaag
    27061 cgccaggatt tggtgatcgt cgtaaagcta tgcttgaaga cattgctatc ttgacaggtg
    27121 gtacagtgat tacagaggat ctaggacttg aattaaaaga tgctacaatg acagcccttg
    27181 gacaggctgc taagattaca gttgataaag atagcacagt aattgttgaa ggttcaggaa
    27241 gttcagaagc tattgctaac cgtattgcac tgattaaatc gcaattagaa acaacaactt
    27301 ctgactttga ccgtgaaaaa ctacaagaac gtttggcgaa attagctggt ggtgtagctg
    27361 ttatcaaagt aggagctcca acagagacag ctttaaaaga aatgaaactt cgcattgagg
    27421 atgctctaaa tgctacacgt gcagccgttg aagaaggtat cgttgctggt ggtggaacag
    27481 cacttattac ggttattgaa aaagtagcag ctcttgagct tgagggcgat gatgctactg
    27541 gacgtaacat tgtgcttcgt gctctagaag agcctgtacg tcaaattgct ttaaatgctg
    27601 ggtacgaagg ctccgtagtt attgacaagt tgaaaaacag ccctgcagga acaggattta
    27661 atgctgcaac aggtgagtgg gttgatatga ttaaaacagg aatcattgac cctgtcaaag
    27721 taacacgatc agcgcttcaa aatgcagctt ctgtagctag tcttattttg acaacagaag
    27781 cagttgttgc taataaacct gaaccagcta cgccagcgcc agcaatgcca gcaggtatgg
    27841 atccaggaat gatgggcggc ttctaagctc tctatagcat taaggattat gaaaaagaag
    27901 ttgagactaa aggtctgctt cttactatga aaaaagaaca aagattggat ttcgactcat
    27961 gatatcccat ctttgttttt ttagttgtga tataaaatgc caatctttgt ggttacagac
    28021 ttttaatgta ttggaaaata cattgttttt ctttgtcatg tgctatcatg gagtcaatga
    28081 aaagttattc ccatatatgg gtgttggagg tctgtgatga atcattttgg agagatttta
    28141 aaaaaccttt agagaatcaa agggattacg attaaaagat gtcgcaaagg ctggtatatc
    28201 aacctctcag ctgtcgcgtt tcgaaaaagg ggagacggac ttaaccatat cgacatttat
    28261 gctgattttg gatgaaagta acatgcccat tgatgagttt atgtatgctg tccatgattt
    28321 tcatcgtgac aacacaattc aagatagtga tggcttgaag cagttacttc atgctcaact
    28381 agcatcaact gataagaagg agatgttcac ggatatcttt tggcatatcc agcatgtcta
    28441 agtctgctat ggcatgctat tttgggagtc cctcacgcga ctctgtcagt agctctcctt
    28501 caaaggattc acatagaaag tcataataga catagccctc tttttttgtc aggctggata
    28561 aagctagaaa taccctttaa ttgaaggttt aaagctgtca gatctatctc ttcttttagt
    28621 tctcataaag ccgcttcaaa gaaagattcg cagggctcga ctttaccttc tggctggacc
    28681 catcctggga agttatcgtg ttgtctatgg agtcataaaa tcatatcgtt atgtttgaca
    28741 cacatattgc cccagtctct aaccgttgct atcaccagat tcaccacctt ttagtatagt
    28801 ataagaaaaa aggggtgaaa gaggtttctt gttgtttttt tggcttaagg tttctttaag
    28861 tcttatctag tatactaatg tcattcctta ataggataag tatttggttg tagaagtatc
    28921 gtcttaaact aatgctatct caacccgatt cccatcttat tcacccctcc tataccgata
    28981 aggtttcaca aagtgtgaga ccttattttt tgacttcagc tctagttatg gagaaacttg
    29041 ttgatacaag cttttttggg ttatggtatt atgatctatt caagcctttc ttgtttttgg
    29101 gtttttcttt aaccaacttg gcttcaatag cttttaagcg tttgaaggtt ttttcagcgc
    29161 gatctagtga aatcttatca gctttttctt gagcagcttt ggggtcactg acgactaagt
    29221 cactgatttc tttggtgctt ttgtcttgtt cggctatcca ggttttttcc aagcctttca
    29281 tctcatcaat cactttagtt ccaaatggtt ttggatgagc agccacgaga tcattaatgt
    29341 gtgacaccgt ccaataccat gacttatcgt tatattgggt gcttttttct tgataggctt
    29401 cataggttct actgatattg ccaaggtatg gcaagtaagg ggcattacga ggactgccaa
    29461 tagatagcca catgacacca cccaattcag cagggatgtc ttttttcagt tggaaaatat
    29521 gagcttccat gacattagga tttgatatgg gataagcata acctttaaca gcttttttgg
    29581 attttggttt acccttaccg tctaatgcca tctgatcaag tggtttaaga tccagcccct
    29641 caaaacgatt acgttgcagt ttcatcgcat cttctagact gaaggtctta tcagtgcttt
    29701 ggagcaactc ataattgctg tctttataag ttacttttga atcagggtca agtgatttaa
    29761 tccctgagaa agagcgagag cggttggcat cgtttagtgg tggattgtaa gatttggcaa
    29821 tatgaaattt accatcaact tcggtataag atttggcttt tttagcgact ttttccacat
    29881 cttctgaggc aatcgtattt tccttatcat tgaaatcgac atgtccgaga tagaaggtat
    29941 ttggaaaaac agcgtattta tcatctggga atttgatggc aacgtattgg tgaccagaga
    30001 gaatttccat gtaccagatg ccgtctttgt cagccaaggt aacaatattt ccttcagcag
    30061 ctcctttttc agtgactatt ttagcgatca atgcgacacc ttcacgggct gttttgacac
    30121 ttggtaaaat gacactggtc atagatgatt ccgctaagcc atttttgacg taaggatcaa
    30181 ttttttggat ggcatcatta gctgaagctg atactgttgc agacatagag acgccaaact
    30241 cattaaaacc agcttcgtca taaacgcctt tgttaggtgt tacatcagga atcgcactat
    30301 agcgataaga atgctcaggc aacgggtact caaaaccatt agagaggtct ttccattttt
    30361 cgccagcagg attgtccttt gctagacgta caatgaaatt tttattatgg tgtggctcaa
    30421 gatcttctgt tcgaccataa agaagagaac cgtctttggt taaatctttg ccaataataa
    30481 aaccagtaca agcataacta acactttgaa gcgaaaaggc tgtcagaata cttaaaactc
    30541 caagtgatat ttttttgttt atcatgcgaa tcctcctagt aaacgataac gctttcacag
    30601 acattataaa atgctctttt taaaaattca agtcttttat gaagtgtttt gaaattgtta
    30661 gctatctaaa tattatgaga attctctctt gaattttggc ttaacggccc ctaaaaatgt
    30721 ataggcagtg aactatctag gtttcttcga aaatccatta gccatttatg atataatagt
    30781 aaagaaaaac atagaaagtt ggggtctgtt gatgaccaaa ccatttcatc ataagaaact
    30841 aaaacaaata actataatag ctgcaactag ccttttttta ttcctgatcg gtggtgcctt
    30901 ttactactct aaaaatcatt gtattaatgc ctatcttaaa gctcgatcgg cccaatcagg
    30961 accagttttt gagaatatca aagcatatct agtctgggat gatactaatg agcagatcac
    31021 aaatgacgag gcgatgtata ctaagtttag aaggtatagt cagaaagaat tgaggcaaaa
    31081 aaagcaggat ttaaaagccg ctagtcaaga tagtgccgtt caagtaaagt ctgttggtcg
    31141 tcgtttttgg atttttcctg attatcggat tgccataaaa cccatggatt taacgataaa
    31201 aacgaatgtg cctcaagcag acgttctttt aaatcaaaaa aaagttgctg tttctgattc
    31261 agaacagttc tcagtcaagc ttgatcggct accaacggca gaatataccg caagtatcag
    31321 aggcaaacac aacgggcgaa acattaaagt caacaaatca tatgatggtg ataatcccgt
    31381 gctagatttg agtgtgtctt ttagaacttt tttggtaaca agcaatgcta agcaaggaga
    31441 tctttacttc gatgataacc atattggcac attaaaagat ggtcaattac aagtagaaga
    31501 ttaccctgtt acagaaaacg cacaagctta tatgaaaaca acattcccag atggtgagtt
    31561 aagatcacaa aaatatgctc tagctgatgt tgaagaagga gcaaccctgg agattttagt
    31621 tacagatctt ttagaagagg acaaggcagg ggagctatta gtatcagctt ttgatcagct
    31681 gatgcactac ctcagtacag gtcaagactc atctaactta cgtagcgtct ttgaggcagg
    31741 gtctagcaac gcattttata ggggattgaa agagtccata aaagcgaaat ttcagacaga
    31801 tacaagaaaa gccagccgtc tcaatattcc atctatcctt ttgacaacaa tgactcaagt
    31861 gggtaaaaca acttacgtgc ttgatttcac agctacctac gaatttttgt atgacaagtc
    31921 aacagatcct gagcagcata cctctggaca tattaatcaa gacttgactg gaaaagtgac
    31981 tgtaaaaaaa gttggacagc attaccttat cagccagtcc ggctccaaaa atattactgt
    32041 tgttaaagag gacaatcaac tcaaagcgcc atctgtcttt cctgagtcta ttttgggaac
    32101 gtggacaggc caagccaatg gtttgagcat ccatatgtct ctagcatcag atggaacaat
    32161 tacgactaaa gttgaagatc aaaaaggcaa ccgttctaaa gaaactcgga cagctaaaat
    32221 tagtaaagtt gaagacaaag gcaatggttt ttatctttat acgccagatc ctggaagtga
    32281 cataagcgcc ttagttccag aaggaggatt ggggggtgca aatgtcaaat atgcttatgg
    32341 tttcaaaata tctggtaaaa cagcctctcc agtggtgtgg caggcagcat taacacatga
    32401 atttgattat accaagccac tttcgggagt aactttgcaa aagcaaccat aaaggatgca
    32461 aagccacaaa gaacagtaga aacaatgtta tcgctcaaat acagtgctat gaaaacagat
    32521 aaactcaaaa ggctaggaga ttatttccta gccttttgag cgtttaagac tgcgtcaaat
    32581 gtagctgatt tagcttgcac gcaaatgtct tcttgggtta gagggtgttt caaacgtaat
    32641 tgataagcat ggagcatcag tcgagcacaa tctttttttc cattggaata caaaggatca
    32701 ccaaataaaa catgaccttg atgggctaaa tggactcgaa tttgatgagt ccgtccagtt
    32761 tgtaattgac aagtgagtaa gcttgccgtt ttgtggaagt ttttgactag agttacctca
    32821 gtaatagctt tcttgccatt tatcggatcg acaacacgtt ttctgcggtc atgtcgatga
    32881 cgaccaattg gatgatggta agttacccta ggactgtcta aggatccgtg aactagtgcc
    32941 aaatattctc gatggatatc tcgtttttct aaaagacgat taaggattgg taagataaaa
    33001 ggtgttttag caaataaaat ggccccactt gtttccttat ccagacgatg aaccacatag
    33061 caggtctgtc ccgtgtaagc agacacgtgg ttgagcagcg ccaactctgt tggatcattg
    33121 ccgtgtgttt tcatcccttc aggtttgtta acaataatga tgtgctcatc ttcataaagg
    33181 caggttactt tttcggcctg ccccatcacg ataatctttt caggataatc ttcgtgatca
    33241 aaaaaaagtt tgacctgatc accgtattta acacaacttt gccagttaac agaatgacca
    33301 ttaataagaa catgtttttt agttctcaga aaatgcctaa ttttacgggg aattaacaat
    33361 tgttcctcta ataatgcttt gaccgtcaat tcttcaaagg gatttttcag tgtgacagta
    33421 aacccctttg atggtttatt tgttgtcttc attgagccag ttatggtatt tttcaacaag
    33481 gttgatagcc atttgggtgg caagagcagc agagaaagct tcagtacctt cacgctcgtc
    33541 attagctacc tccaagcgag tctcatcgta aatggctttt agactttctg cggataaggt
    33601 ttctttaaaa gcttggaatt tattcattgg ttaaacccgt ccttctatgt ttaatgtgag
    33661 ctcctgatta atgcaaggaa ttcaatgcta ttatagcatg tttctaatca aaacgtgcca
    33721 ctcaaaaagg taaaaacgtt attttgctaa tccatctaat aagaaagtgt cttaaataag
    33781 acctcttgat agagtttgta acagaaatga tatgaaacta ggtgctaacg cagaaataga
    33841 aaacaactaa cgtgttgcga agttggaaca gtcataaaaa atagcaagat aaaacttatc
    33901 aacacatagt tacctaagac gatgagtcaa acaggtatgt tctttcaaaa caaccccatg
    33961 aggttgaacg acgtatcgga aaaatacgga ttagtttggc cttatgatag gtatttctct
    34021 atcgtaaaag aagtcaacca aaggttttta atataaaata ttaagaaaaa ttgttataat
    34081 tgtgataaaa acataatcgt aaaatagagg aaaaacaatg aaaacaatta gacgttatga
    34141 tgttaacgaa gatagagggc atacaggcct tgtagaagca ggagatttct attatttaaa
    34201 ctattgtgtc ggcaatgttg gacaagacat tgaaagtcag attaatggag cctttgacga
    34261 gatggagcgc cgattagcat tggtggggct cacattagat gcagttgttc aaatggattg
    34321 tctatttcga gacgtttgga acatacctgt gatggaaaaa atgattaaag agcgctttaa
    34381 tggcagatac cctgctcgaa aatcgattca aacggaattt gcacatcacg gtggaccaca
    34441 aggactactc tttcaagtgg atggtgtggc ttattcaaag catatttcga tgacctaatc
    34501 aaggttactt taagatcata gtggtataat aacgttatga gatttctaga acttttacaa
    34561 aagaaatttt ttcctaaagc atatcaggaa aaacaattct taatgcatca aaaaacgcgt
    34621 ttaacgccac aacacaatca aaagcagtat tcgccaaatg ccaatcattt ggactcatca
    34681 gctaccaaaa actcagaaca agaccctgca acagctctgc aacgcagtag agcctacgaa
    34741 ggaagcccta aaagtcggcc cgcttggttg caaaagctgg aagctgtttt gccgtctcct
    34801 caacgtccaa ttcggcgttt ttggcgccgc tatcacatcg gaaaactgct aatgattctg
    34861 attggaactc ttgtcttact cttaggatca tacttgtttt acttatcaaa aacagctaaa
    34921 gtatctgatt tacaagatgc cttgaaggct acaacggtta tttatgatca caaaggagag
    34981 tatgcaggca gtttatctgg tcaaaaaggg agttatgttg agctcaacgc tatttcagat
    35041 gatcttgaga atgctgttat tgccactgag gataggactt tttacagtaa tagcggtatt
    35101 aatcttaaac gcttcttatt ggcggtagtt acggcgggcc gctttggagg tggctcaacg
    35161 attacacagc aactggctaa aaatgcttat ctctcacaag atcagacaat taaacgaaag
    35221 gcccgagagt tttttttggc gttagagttg accaaaaaat acagtaaaaa agatattctt
    35281 actatgtacc ttaacaactc ctactttgga aatggagttt ggggagttga agatgccagt
    35341 caaaaatatt ttggaaccac agctgctaac ttaacactgg atgaagctgc cacattagca
    35401 ggtatgctca aaggacctga aatatataac ccttaccatt ctctaaaaaa tgctactcac
    35461 cgtagagata ctgttttagg agcgatggtt gatgccaaaa agattaccca aacaaaagct
    35521 cagcaagcta gagcagtagg gctaaaaaat cgcttagctg atacttatgt tggtaagaca
    35581 gatgactaca aatacccatc ctactttgat gctgttatta gtgaagcaat agcaacttat
    35641 ggtctttcag aaaaagacat tgttaataat ggatacaaag tttacactga gctagatcaa
    35701 aattaccaaa ctggcatgca gacgactttt aacaacgatg aactatttcc tgtttcagct
    35761 tatgacggta gctctgctca agcagctagt gttgctttag atcctaaaac aggaggtgtt
    35821 agaggtctga ttggtcgtgt gaatagtagt gaaaatccga ctttcagaag ttttaactat
    35881 gcgactcaag caaaacgtag tcccgcatca acaatcaaac cactcgtggt ttacgcgcca
    35941 gccgttgctt caggatggtc aattgaaaaa gaactaccaa ataccgttca agatttcgat
    36001 ggctatcagc cacataatta tggaaattat gaatcagaag atgttcctat gtatcaagca
    36061 ttagcaaact cttataatat tccagcagtt tctacattga acgatatcgg aatcgataaa
    36121 gcctttacct atggtaaaac atttgggtta gatatgagct ctgccaaaaa agagttgggg
    36181 gtagctttag gtggcagcgt gacaaccaat ccattggaga tggctcaggc atatgctgcc
    36241 tttgccaata atggagtaat ccatcctgcg cacttgatta accggattga aaatgccagg
    36301 ggtgaagtgc ttaaaacctt tactgataag gctaaacgtg ttgtcagcca gtctgttgca
    36361 gataagatga cagccatgat gctaggtacc ttttcaaatg gaacagcagt caatgctaac
    36421 gtatatggct atacactagc tggtaaaaca gggacgacag aaaccaactt caatcccgac
    36481 ttagcaggcg atcagtgggt tattggttat acgccagatg ttgttattag tcaatgggta
    36541 ggatttaatc agaccgatga aaatcattat ctaacggatt caagtgcagg cacggcctca
    36601 gctattttta gcactcaggc atcttacatt ttgccttata ccaagggcag ccaatttcat
    36661 gtagataatg cctacgctca aaatggtatt tcagctgttt atggagtcaa tgaaacaggt
    36721 aatcaatcag gagttgatac tcaatctatt attgatggtt taagaaaatc agcacaagaa
    36781 gcttcgcaat cactatcaaa agcagtcgat cagtcagggt tacgtgataa agcccaatct
    36841 atttggaaag agattgttga ctattttaga tagcttgcag tattttagag atcgtgttaa
    36901 aataatagtt acggaggcgt tatggcacag aaaaaagcga gtctagcgtg tgtcgaatgt
    36961 ggcagtcgta actactctat cggagtgagt agcacgccta aaccaacacg tctagaagta
    37021 aataaatttt gcaaatactg caaaacatat accttacaca aagaaacacg ttaaaaagga
    37081 gagcctgatg ggattcatta gcgggacttt taaagtctta aaagacacaa catggccaaa
    37141 tcgtaaacaa cgttggaaag attttatctc agttcttgag tacacagctt tctttacggt
    37201 gattatctac atctttgatc agctcttagc aaagagtgta ttagcactga ttaatttatt
    37261 ctaggtcaag attggtagaa atgataagct tgcttggaaa agcctaggct gggagtattc
    37321 ccagcctttt ttgtagtatc aataatctga ttggtaagct tttttaaagc ccatactggt
    37381 ttgagcttta aaaaagcata aaggtaaagg caatgtagca acgatctcca aaccaagtgc
    37441 actaaattag cttgtaacag agtcaacgca taaacagcct ggcctcactt gcttagcaca
    37501 ttaaaaatag caatgaatat tatggttgag gaaaacagcc tgatagtctg ctaaatcttc
    37561 gggatgataa gccttgacct cagccatctc atactcccta cctagaagtt tgtacttgtt
    37621 ttctccaaat tgatggaaag gtaacagttg cacttggtca atctcaagct gattaaagag
    37681 ctctgagaag gctttagcat cgtctaggga atcattaaat tggggaataa cagggatgcg
    37741 caagacgatt tcttttccag cttgaaaggc ataatgaata ttcttgataa tcaagtcatt
    37801 acgaacccca gtcacttttt ggtgtctgag ctgattataa tgtttcaaat cggtatagat
    37861 aaagtccacg tagtcaacaa gggtgacaaa ttgctcgtgt ttggcaaagg cggtggtttc
    37921 aattgcggtg tgaagacctg ctgctttggc tgcttttaac agggctaagg caaagtcaaa
    37981 ctgagcaaag atttcccccc ctgacagggt catgccccca cctgactctt cgtagaaatc
    38041 aaggtcttta aggacttctt cgatcacctc gtccacggtt ttttcctctc caactatttt
    38101 agtattaagc ccatcacttg ttaacatctg ctcaggcgct ttttgttggg attcgggatt
    38161 ggcacaccat ggacatctca aggggcaacc ttttaaaaaa acggttgtcc gaataccagg
    38221 accgtcatga atactaaaat gttggatgtt gaaaacaata ccgcgatcag tcataagtgt
    38281 gaattccttt cgttgtaaaa ctatcatacc agataaacgt aaataaatca attacgaatg
    38341 aaagtaaata tcccttttct tttaagaaaa taactggtac aatgaaaggg aggtgaatga
    38401 tgaatcgatt agaacgtatt attcaactgg tttcccagaa gaaaaaaata gatgttaaca
    38461 gtttatcaga gcaattagac gtttcgaaag taaccattcg aaaagacctt gataagcttg
    38521 aaagtaaggg cttgttgcga cgggaacatg gctatgcggt gctaaacagt ggcgatgacc
    38581 tcaatgtacg cttgtcctac aattacaaca taaagcgacg gattgcagaa aaagcagctg
    38641 aattggtaca agacaacgat accattatga ttgaatctgg ctcaacctgt gcccttttgg
    38701 cagaagtgct ttgccagacc aaacgaaata ttaaagtcat taccaattcg tgcttcattg
    38761 ccaactacat tcgtcaatat agtagctgtc aaatcatttt actgggcggt tattaccagc
    38821 ccaattcaga ggtgactgtt ggtcctttat tgaaagaaat gatatccctt tttcatgtca
    38881 atcgtgtctt tgttggaaca gacggtttca acaaagatct tggctttatg ggcaaggaca
    38941 tgatgcgctc agaaggggtg cgttacatgg cagatgctgc tgaggaagtg gtgattctga
    39001 ccgattccag caagttttca aaaaccagtc tggtacacca gttgtcatta gctgatgtca
    39061 atcgcgtcat tacagaccaa gcactagaca agcaaacaca agagttatta agtgctagtg
    39121 gtcttgttct tgattttgtt tcttagaaaa taggtggcta gatgaaagaa gaacgcagac
    39181 ggctccttgc caaggtggct tacttgcatt atgttcaagg caagagccaa accttgattt
    39241 caaaggaaat gaatatctac cgaacaacgg tttgtcggat gttagcaaaa gccaaggaag
    39301 aaggcattgt tcggattgaa attgccgact atgatgctga tttatttgct ttagaagaat
    39361 acgtgcgaca gcagtatggc ttggaaaaac tagaccttgt gcccaaccag gttgaagata
    39421 ccccaatgga taccttgaca aatgttgcta aaacagcagc tgaggttttt agacatgttg
    39481 ttaaagatgg cgataagatc ggtctgtcct ggggagcgac tctttcttgc ttgatggatg
    39541 agttaaatcc aaaagcgatg aaagatgtgt ttatttatcc tttggcagga ggacctagcc
    39601 atattaatgc caaataccat gtcaataccc tggtttaccg cttggctcgc atctttcatg
    39661 gtaatagcgc ttttatgaac gctatggtca ttcaagaaga caaacacttg gctaagggaa
    39721 tcttacaatc taaatacttt aacgacattt taacaagctg ggaccagtta gatcttgcct
    39781 tagtaggaat tggtggggag cctaatagcc ttgagcagag ccaatggcgt gatttattaa
    39841 ccagctcaga tcatgaccaa ctcaaatatg aaaaagccgt tggtgaagtt tgttgccgtt
    39901 tctttgatca ggcaggtcag ccagtttaca ctggcctaca ggatcgaact attggtattt
    39961 cactagaaca gttgaggcgt gtccccaaaa caatggctgt tgcaacaggt aaacacaagg
    40021 ctaaggcaat attggcagcc ttaaaagcag gttttatcaa ttatctagtg acggataaag
    40081 agaccatgct agctgtttta gcgttagatg aagatattga tcttaataac gttcttcttt
    40141 aatagaaatt caaaaatact atgatattta tagaggccca agaccaaacg gtcagggctt
    40201 tttttgtttg atgtgttgat aaaacagaga ttgctcaaat gtgcagttaa tgtattggca
    40261 caaaagtgca ttaaccaaaa tatagcacaa aatatattga ccctaaaaac aaaaatgata
    40321 caataaactt accaacgaaa tgtaaacgat tacaaacaca ataacatcac cttcgaaaaa
    40381 aagactcatt gccgttacat cacatgaaaa agaaaaaaag gagacaccaa tgcaagttat
    40441 tgtaccagac caaatcatta tgggcttaat tttaaatgca ggcgatgcca aacaacatat
    40501 ctaccaagct ctgaaatgtg ctaaagagga cgattatgcc actagtgaaa aagaaatggc
    40561 cttagcagat gatgccttat tagaagccca caatttacag acccagtttc ttgcccaaga
    40621 agctagtggt aataagtcag agattacagc tctttttgtt cactcacagg atcacttgat
    40681 gacgaccatt acagaaatta atctcatcaa agagattatt gaccttcgta aagaattggc
    40741 aactaaataa gttttcatga ctaaaaagga gattgacatg attaaaattg gattgttttg
    40801 tgcagcagga ttttcaaccg gtatgttggt gaacaacatg aaagtagctg ctgaaaaaaa
    40861 aggcattgat tgtcagattg aagcctatgc tcaagggaaa ttagcggact atgcaccatt
    40921 acttgatgtg gcacttttag gcccacaggt tgcctatact ctggataaat cagaagctat
    40981 ttgtaaagac aatgatatcc ctattgcagt tatcccaatg gctgattatg gaatgttaga
    41041 cggcaacaag gtgcttgatt tagcccttag ccttgttaaa gaataggagt caatcatggc
    41101 aaaaatgaat atgcaaaaca tcatcaagcc aatcatgaca tttgtgaata tgcgcggcat
    41161 cattgcctta aaagacggta tgttagccat tttacctttg accgttgtgg ggagtctttt
    41221 cctgattgct gggcagattc ctttccaagg ggttaatgat gccattgcta gtgtgtttgg
    41281 tgctgactgg acagaaccct ttatgcaggt ttaccatgga acatttgcta ttatggggtt
    41341 gatttcctgt tttgcaattg gctattctta tgctaaaaac tcaggtgttg aacctctccc
    41401 ttcaggggtt ctatccttgt cagccttctt tattttattg agatcatctt atgttccagc
    41461 agaaggtgaa gctattggag atgctatcag taaggtttgg tttggtggtc aagggattat
    41521 tggtgctatt gtgattggtt taacggtcgg agctgtttac acaacattta tccgtcgtca
    41581 tatcgtcatt aaaatgccag atcaagtgcc acaagccatc gccaaacagt ttgaagccat
    41641 gattccagcc tttgtgatct ttaccttgtc aatgcttgtg tatattattg ccaagtcagt
    41701 aacaggtggt ggcaccttta ttgaaatgat ttatgatgtc attcaggtac cgctgcaagg
    41761 gttaacgggt tctctttatg gcgcactagg tattgccttc tttatttcct ttctttggtg
    41821 gtttggcgtg catgggcaat ctgtggtcaa tggaattgtc actgctcttc tcttatcaaa
    41881 tctagatgcc aataaggcct tgatggcagc aggtgagtta tccctagaca agggtgccca
    41941 tattgtaacc caacaatttt tagactcttt cttgattcta tcaggttctg gcattacctt
    42001 tggtttagtg gtagctatga tctttgcggc taaatccaaa caatacaagg ctttaggcaa
    42061 ggttgcagcc ttcccagccc tctttaatgt taatgaacca gtcgtctttg gttttccaat
    42121 tgtgatgaat ccagtcatgt tcttgccatt tatcttggtt ccggtcttgg cagctctcac
    42181 agtttatggc gccattgcta ttggtttcat gcagcccttt gcaggagtga cccttccgtg
    42241 gtcaacacca gccattatct cagggttcat ggttggtggc tggcaaggag ctattgtgca
    42301 aattcttata ctcatcatgt caacgttggt gtacttccca ttctttaaaa tccaagataa
    42361 tatggcttat caaaatgaac aagctagtga agagtcatga tttagcgtcc accaaattct
    42421 tgagaccatc tccagttaga ggtggttttt tagaaaaaga agaacgactt atcaggccta
    42481 tattgcacaa tagtgcaaaa taaagtgact atctcttctt atggttaagg atacgcaaaa
    42541 gtcctaatat atgataaaat agttacgaaa gaaacgttta taatttcgta agaaagaagg
    42601 agaccccgca tgactgaaac aaaaagccct tattttggac atttaacaga cagaatgact
    42661 cactatcgtg aagctgtgtt agacaagaaa ccttatattg atgctgagcg tgctatttta
    42721 gccactgaag cctatcaaaa acaccaaaat aagccagcaa acctcaaacg cgcttacatg
    42781 cttcaaacta ttttggaaaa catgaccatc tatattgagg atgaaagcct cattgctggt
    42841 aaccaagcct cttcaaacaa ggatgcaccg attttcccag aatacactct ggaatttgtc
    42901 ctcaacgaac ttgatctttt tgaaaaacga gatggggatg tcttctacat taccgaagaa
    42961 accaagcaac agctccgaga cattgcccca ttctgggaaa acaataacct gcgtgctcgc
    43021 tgcggtgtct tgctaccaga agaagtgcaa gtgtacatgg aaacaggttt ctttggcatg
    43081 gaaggcaaga tgaactctgg ggatgctcat ttagcggtta attaccaaaa actcttggag
    43141 catggcttaa agggatttga agaacgggca cgagctgcca aagctgcgct tgatttgacc
    43201 attcctgaaa acattgacaa ataccatttt tacgactctg tttttattgt cattgatgca
    43261 gtcaaaactt atgctaaacg ctatgccaag ttggctagag aactagcaaa aacggctaaa
    43321 ccagaacgtc aagcagaatt gttagacatc gcgcgcattt gtgataaagt accttacgaa
    43381 ccagcgaaaa cctttgcaga ggctgtgcaa tctgtctggt tcattcagtg tattttgcaa
    43441 attgaatcaa atgggcattc cctatcttat ggccgctttg accagtacat gtatccttat
    43501 gtcaaagctg atttggaggc tggccgtgaa acagaagaca caattgttga acgtttgacc
    43561 aacctctgga ttaagacctt gaccattaat aaagtacgga gccaagccca caccttctca
    43621 tcagcaggta gtccacttta ccaaaacgtg acaatcggcg gtcaaacacg cgataaaaaa
    43681 gatgcggtca acccactgtc ttaccttgtc ctacggagtg tggctcaaac caaattgcca
    43741 caacctaact tgactgttcg ttaccacaaa ggcctagaca atacctttat gaacgaatgt
    43801 atcgaggtca tgaaacttgg ttttggtatg ccagccatga acaacgatga aatcatcatt
    43861 ccatctttca tcaaaaaagg tgtctcagaa gaagatgctt atgattactc agccattggc
    43921 tgtgtggaaa cagcagttcc tggaaaatgg ggctaccgtt gcacggggat gagttacatc
    43981 aacttcccta aaatcttgct gattaccatg aatgacggga ttgacccagc atcaggcaag
    44041 cgctttgcaa aaggacacgg tcacttcaaa gacatgacct cttatgaaga attaaaagca
    44101 gcatgggatg ccaccctgcg cgaaattacc cgcatgagcg tcattgttga aaatgccatt
    44161 gacctcggtc ttgaacgtga agtaccagat attctttgct cagccttgac agatgattgt
    44221 atcggtcgtg gcaagacctt aaaagaaggc ggagctgttt acgactatat ctcaggcctt
    44281 caagttggta ttgccaacct atcagactcc ctagcagcgc tgaaaaaatt agtgtttgaa
    44341 gaaggtcgct tgaccccaga ggaactctgg aaggcccttg aaagtgactt tgctggagaa
    44401 cgcggagaag acatccgcca aatgctcatc aatgacgcac caaaatatgg taatgatgat
    44461 gactacgcag atagtttagt cgtcgaagct tacgacacct acattgatga aattgccaaa
    44521 tacccaaata cccggtatgg ccgtggccca atcggaggta tccgctattc tgggacatca
    44581 tcaatctcag ccaatgtcgg acaaggaaaa gggaccttgg cgacaccaga cggtcgccat
    44641 gcaggcacac cactagcaga aggctgttca ccagaacaca gcatggacaa aaaaggcccg
    44701 acttcagtat tgaaatctgt tgctaaatta ccaacagatg agattgtcgg aggagtgcta
    44761 ctcaaccaaa aagttaaccc acaaaccctg gccaaagaag aagacaaact gaaattaatg
    44821 gccttacttc gtaccttctt taatcgccta cacggttatc atatccagta caatgtcgtg
    44881 tcacgtgaaa ccttgattga tgcccaaaaa catcctgaaa agcaccgcga cctcattgtc
    44941 cgtgttgccg gctactcagc tttcttcaat gtgctctcaa aagcaaccca agatgacatt
    45001 attgaacgta cagaacacac actttaggag aaaataagta tggaatacat gttagacact
    45061 ttagatttag aagctattaa aaaatggcat cacattttgc ctctcgcagg tgtgacctca
    45121 aacccttcta ttgccaaaaa agaaggtgag attgatttct ttgaacggat tcgagaagtc
    45181 cgtgctatta tcggtgacaa ggcttctatt catgttcaag tgatcgccca agattacgaa
    45241 ggcatcttaa aagatgctgc tgaaatccgc aggcaatgtg gagatagtgt gtatgtcaaa
    45301 gtacctgtaa ccacagaggg ccttgcagcc attaaaacct tgaaagccga aggctatcac
    45361 atcacagcaa ccgctattta tacgaccttc caaggactat tagccattga agctggcgct
    45421 gattatttgg ctccttacta taaccgtatg gaaaatctta atattgatcc agaagctgtc
    45481 atagaacaat tagcagaagc tattaaccgc gagaatgcta atagtaagat tttggcagca
    45541 agctttaaaa atgtggctca ggttaataaa tcctttgctc taggagcaca agccatcact
    45601 gctggcccag atgtgtttga agcaggattt gccatgccat ctattcaaaa agcggttgac
    45661 gattttggca aggattggga agccgttcat caccgcaaga gcatttaaga aatagcatca
    45721 gtagtaatca catcattagg aatcacagat aaaggagttc aacgatgaaa gtatttgcaa
    45781 gtccatctcg ttacattcaa ggtaaaaatg ccttgtttac caatgttaaa accctaaaac
    45841 aactagggga tagccccatt ttactgtgtg atgacgtggt gtatggcatc gtcggagaaa
    45901 gatttgaaag ctatttgata gataatggca tgactcctgt tcacgtagcc tttaatggcg
    45961 aagcctcaga taatgaaatc agccgcgtgg ttgccattgc caaggaaaat ggcaatgatg
    46021 ttattattgg acttggtggt ggaaaaacca ttgacagtgc taaggctatc gctgacttac
    46081 ttgctgttcc ggtgattatt gccccaacca ttgcttcaac agatgcccca acctcagcct
    46141 tatcggttat ttacaccgat gaaggagcct ttgaaaaata cattttctat tcaaagaatc
    46201 cagatcttgt attggttgac acacaagtga tctgtcaggc accaaaacgc ttattggcgt
    46261 cagggattgc agatgggtta gcaacatagg tagaggcgcg tgctgttatg caaaaaaatg
    46321 gagacaccat ggcaggtggc aatcaaacct tggcaggagt tgccattgcc aaagcctgtg
    46381 agcagacctt gtttgcagat ggcctaaaag cgatggctag ctgtgacaga caagtcgtga
    46441 ccccggcctt ggaaaatgtc attgaagcca atacacttct tagtgggctt ggttttgaaa
    46501 gtgcgggtct ggctgctgca catgccattc acaatggctt tacagcgctg acaggtgcta
    46561 ttcatcacct tacccatggt gaaaaagtcg cctatggcac gttgacccaa ctcttcttgg
    46621 aaaatcgttc gcgtgaagaa attgaccgct acatcgactt ttaccaagct attggcatgc
    46681 caacaaccct caaagaaatg catttagaca cggctactca agaggatttc ttaaaaattg
    46741 gccgtcaagc aaccatggca ggcgaaacca tccatcaaat gccatttgtt atcagtccag
    46801 aagatgtagc agcggctttg gttgctgttg atgcctatgt tacgagtcga taggctaacc
    46861 aaaaagagat ttcttatttg ccagataacc tcaaaaaaac aagtctaggg agtcacttcc
    46921 tagacttgtt ttgcttattg ttggttggta atagcacttt tagcggggtc attgtgggca
    46981 atacgactgg cagctgctgc cgcaatggcg ctaacaatat catctaaaaa ggtatggcag
    47041 gagtagccat ctttgtggtt gagtttgtca actattcccg gttttgtctt gtccagatac
    47101 ccataattgg taaaaccgat agacccatag aggttaacaa tggataaggc caggatttca
    47161 tcaatcccat acagcccttg gtcggttttc aagatactaa ggagtggctc agacagctgg
    47221 ttggcttcag ccaatttatc caattctacc ccagtgataa tagcattttg cacttctcgt
    47281 tttgcaagca ccgcctcaac gctctctaaa cattctgcca tggtcaaatt gggaatataa
    47341 tcattctgca aaaaaagcac cagctctgca atagcttcta aagaaacccc tcgctcagcc
    47401 agtaattgat aagaaacttc tcgtaattga tggtcagttg acatgtgaaa cctccttttt
    47461 ttctttattg taacataaaa gattttaaga aacctctaca acagctaaag agggaatagt
    47521 tcttttttaa gaggtgttga ctactgaaat cccctacttg gtaaaagtca aagaaacgat
    47581 agtaaagtaa ccatcttacc taggcctaaa tacaatcacc acctctaggt gacacacagc
    47641 acctatttca aaaatgatat tagtatggca aaacaaaaag acaacgcctt cttttttctc
    47701 cttactatct cctttaattt tcatattttt taaaaaaact attgataaac tagttaagta
    47761 agcgtatact atggttagtg agcgaaatta gaaaagagga caagcatatg aatctacttg
    47821 gatcaagacg ggttttttct aaaaaatgtc ggctagtaaa attttcaatg gtagctcttg
    47881 tatcagccac aatggctgta acaacagtca cacttgaaaa tactgcactg gcacgacaaa
    47941 cacaggtctc aaatgatgtt gttctaaatg atggcgcaag caagtaccta aacgaagcat
    48001 tagcttggac attcaatgac agtcccaact attacaaaac cttaggtact agtcagatca
    48061 ctccagcact ctttcctaaa gcaggagata ttctctatag caaattagat gagttaggaa
    48121 ggacgcgtac tgctagaggt acattgactt atgccaatgt tgaaggtagc tacggtgtta
    48181 gacaatcttt cggtaaaaat caaaaccccg caggctggac tggaaaccct aatcatgtca
    48241 aatataaaat tgaatggtta aatggtctat cttatgtcgg agatttctgg aatagaagtc
    48301 atctcattgc agatagtctc ggtggagatg cactcagagt caatgccgtt acagggacac
    48361 gtacccaaaa tgtaggaggt cgtgaccaaa aaggcggcat gcgctatacc gaacaaagag
    48421 ctcaagaatg gttagaagca aatcgtgatg gctatcttta ttatgaagct gctccaatct
    48481 ataacgcaga cgagttgatt ccaagagctg tcgtggtatc aatgcaatct tctgataata
    48541 ccatcaacga gaaagtatta gtttacaaca cagctaatgg ctacaccatt aactaccata
    48601 acggtacacc tactcagaaa taataccaaa aggctagacc tctgctcact aggcctagct
    48661 ttttacatca aaaaaagcaa tgactataga aagtaaaaat actagaaaaa gcaatgattg
    48721 tcgtcattgc tttttatgat ttgtcaaaaa gtaaaaagca aattgaaaaa atcgccctgg
    48781 agctgttgag ataaactaca ccatttatta agataccaaa tcaaaaagct aacaccataa
    48841 gagcgttagc tttttaaaga gcctcaggac agtttatgtt taatggcttc taggtaggtt
    48901 tgaaacatat gatggatcgt tttgcaatta agtaattgga agatctccaa gatctgcaca
    48961 cacttttgct cagccccctc tttaccattt atgaggccaa taatgccatc aaaaaaagac
    49021 acacaacacc gttcatataa gcaatcatca tttagctgat aatcttcaat ttttgccaaa
    49081 atctcagaag ctttatcata ctcttgtcgc tgaataaaca aaatcaacat attgacaaac
    49141 atccgaatcg attcattccc ataatacctt agggtattgt atttatcgag gttcaaaata
    49201 acttttgaaa acaccatctc aataaagcaa gactcaaaaa taaacataca attattaaaa
    49261 agcacagtct catagtgact ccaagtttca atattaataa gatagtttga caaataagtc
    49321 cgctctgtca gacagtcttc ctcagtaaga gttgctaata acaccttgac caaggcaaaa
    49381 aggttctttt cctttgttga cttactatct ttataagaat caaggatatg atttaaacct
    49441 tctatgtttc ggcattcaaa ataatgtttt gccgtatcca tatcaataaa ttctttgtat
    49501 tgtttaaagt tattactaat aaagagaaac tcatcaacat tgacattcaa gttgtctaag
    49561 atataaagta actttttcga agaaatgtca agattatttt taataaaacg ataataggtt
    49621 tgcctagtga gataatcacc acaaacttgt ttaatcgaaa tgttttttga atgcctaatg
    49681 aattcaacgg tttcaccaat ttccatatgt caagccttcc tagttgatgt caaaaatacg
    49741 ttacgcatgt acctaatacg taacaagttg aatgtttcgg atgatagtcg cttatgatag
    49801 gtgcataagg tcaatagcca gatgcgatag ttttatcaac tgtcatatgt taaacctttc
    49861 taatctaata gatgttaaaa atacgttacg tgtgtaccta atacgtaaca aaataatggg
    49921 ttagcaaaat aagcagctat gatatagcca taaggttaaa aggaggcgcc tactatgtgg
    49981 ttattgttac tatttttgta gttccttttg caaaatggta gtagataaaa atgagaagtc
    50041 aataaaagca ttgacaaaca aaagaatcat tttgtttata ttatcagtat ttttaagttt
    50101 cttgtcaact gaaatgagca tctactagcc acaatagtaa ctaaaaggct aattgtcagt
    50161 aaaaatgaga taaatcagcc aggaaaaagc ctacgagtca actaatggac aacccaaaaa
    50221 aagacaaagg actttcactt ggtgttgtgg accctatcac gatccttatc acagaatcag
    50281 ttattaacaa aatgagaacg gtcccatatc atctaaaaaa ctgtcaagca caacctattg
    50341 tttgctagct gatcttgcct atcagtctgc ctagtagata cggcagaaga taagagatca
    50401 accaaaagat gataagtgca acgactcata gcgtcttttg accaaaacca attaggctca
    50461 catgagatga gaataattgg gttgggttgt cagtgtcaac taaccgtgtt attgtctatt
    50521 accattcatg gtatcagcga catcgtatga taaccatacg attcagctaa gtaaggaggt
    50581 gtgtccaatg taccgttaaa agcaaatgca gtagattaac ttattttgaa agaggtataa
    50641 aaaaaatgaa taaaaagaaa ttaggtgtca gattattaag tcttttagca ttaggtggat
    50701 ttgttcttgc taacccagta tttgccgatc aaaactttgc tcgtaacgaa aaagaagcaa
    50761 aagatagcgc tatcacattt atccaaaaat cagcagctat caaagcaggt gcacgaagcg
    50821 cagaagatat taagcttgac aaagttaact taggtggaga actttctggc tctaatatgt
    50881 atgtttacaa tatttctact ggaggatttg ttatcgtttc aggagataaa cgttctccag
    50941 aaattctagg atactctacc agcggatcat ttgacgctaa cggtaaagaa aacattgctt
    51001 ccttcatgga aagttatgtc gaacaaatca aagaaaacaa aaaattagac actacttatg
    51061 ctggtaccgc tgagattaaa caaccagttg ttaaatctct ccttgattca aaaggcattc
    51121 attacaatca aggtaaccct tacaacctat tgacacctgt tattgaaaaa gtaaaaccag
    51181 gtgaacaatc ttttgtaggt caacatgcag ctacaggatg tgttgctact gcaactgctc
    51241 aaattatgaa atatcataat taccctaaca aagggttgaa agactacact tacacactaa
    51301 gctcaaataa cccatatttc aaccatccta agaacttgtt tgcagctatc tctactagac
    51361 aatacaactg gaacaacatc ttacctactt atagcggaag agaatctaac gttcaaaaaa
    51421 tggcgatttc agaattgatg gctgatgttg gtatttcagt agacatggat tatggtccat
    51481 ctagtggttc tgcaggtagc tctcgtgttc aaagagcctt gaaagaaaac tttggctaca
    51541 accaatctgt tcaccaaatc aaccgtggcg actttagcaa acaagattgg gaagcacaaa
    51601 ttgacaaaga attatctcaa aaccaaccag tatactacca aggtgtcggt aaagtaggcg
    51661 gacatgcctt tgttatcgat ggtgctgacg gacgtaactt ctaccatgtt aactggggtt
    51721 ggggtggagt ctctgacggc ttcttccgtc ttgacgcact aaacccttca gctcttggta
    51781 ctggtggcgg cgcaggcggc ttcaacggtt accaaagtgc tgttgtaggc atcaaacctt
    51841 agtatggaaa tgcatttcgt tagaacagaa cctgaggcac gccgcatagc tgaaaccttt
    51901 tgtgccgaaa acacacaaac aaaaacccct atgcgcgtgc agcagttaag ctatccatca
    51961 gacacagatc actcaggtgg tgagctatac atctatgctt tgtctcctgc tggatttatc
    52021 atcgtatcag gagacaccag agcgcacacc attttaggct attcttttga taataaccta
    52081 gacctcaacc atgataatgt caggagtatg atagaagctt accaaaaaca aatcaactct
    52141 ttggattgat cggtcaaagt agcttttgcc ccctcaccac tcgtttaaga cgaccgtgtt
    52201 tctaaaatac cactaccttt taagtttcac gccgacctaa gcaagtgttt gtctcatgga
    52261 gcagactgtt tccatcgaaa agtcacccat tgataaagga ggtcatcaac gcctactaat
    52321 gatgacttcc ctaagatgcc aaggtaaagg actgagttct gtagagatgg gactcagtcc
    52381 ttttacgatt tttgagagtt attctagcca taccgtggca agaaaacaac actcaaaaat
    52441 agccaacgtc agattctgtt catacatgac tacagccaaa caccacaacc taacctatcc
    52501 taaagacagt ggctttctaa cgaacagtca caacgagcag caacaccaaa aagcaagatt
    52561 aagctacgtt taagactaat gatataagat aacagtaatt attaatgaga aaagaggctc
    52621 aaaaggacat gaaacaaatg aataaactca ttacaggagt ggtaacgctg gcgacagtcg
    52681 tgaccttatc agcttgtcaa tcatcacaca acaacaccaa actcgtctcg atgaaaggag
    52741 acaccatcac tgtcagtgac ttctacaatg agaccaaaaa cacagaactc gcacaaaaag
    52801 ccatgttaag cttggtgatt agccgcgttt ttgagacaca atatgccaac aaagtctctg
    52861 acaaagaggt tgaaaaagcc tataaacaaa ccgcagacca atacggtaca tcctttaaga
    52921 cagtcctagc acaatcaggc ttaacgccag aaacctataa aaaacaaatt cgcctcacaa
    52981 aattagtcga atatgccgtc aaagaacaag ccaaaaacga aaccatctca aaaaaagact
    53041 accgtcaggc ctatgacgct tataccccaa ccatgaccgc agaaatcatg cagtttgaaa
    53101 aagaagagga tgccaaagca gcgcttgaag ccgtcaaagc tgaaggggca gactttgcag
    53161 ctattgccaa agaaaaaacc actgcagccg ataaaaaaac aacctatacg tttgactcag
    53221 gcgaaacaac cctaccagca gaagtagtta gagctgcatc aggcctcaaa gaagggaaca
    53281 gatcagaaat catcacagcg cttgatccag ccacctcaaa acgcacctac catatcatca
    53341 aagtcaccaa aaaagcaact aaaaaagcag actggaaagc gtaccaaaaa cgcttgaaag
    53401 acatcatcgt gactggcaaa ttaaaagacc ctgacttcca aaacaaagtc atcgctaaag
    53461 ctcttgataa agcaaatgtc aaaatcaaag acaaagcatt tgccaatatc ttagcccagt
    53521 ttgcaaaacc taaccaaaaa caacctgccc aaaaatagag ggttgccagt ttatttccct
    53581 aaggttgctt aaaaagacag ccctagccat cactcccctt ttcaaaacac cgataatgga
    53641 aacgtgtcct atactgatgg tctagggcaa tgtcataaag ctaaagactc tatccaaata
    53701 aggatagagt ctttaatatt taaaaaccag tcagcaaaaa aaattcacgc taagcctagc
    53761 tagaatgagg cttagcgtga atgttatcag tgatagtccg ttaacatagc tgattgggag
    53821 tcagaaactc taactaccca atctgactat acccccagta ccagaggtgt ctcatcgcag
    53881 aagtagcaat cagataatcg tctgataagc tcttgtcctc aagcaactga tgaatatcgt
    53941 cgcacgggcc atgagctgcc ggtggctgat agctagtagg ctcatcagcc aaaacttttt
    54001 tggagtaacg ctttggtctc agacggcgcc agagggttga aagaggggtg agaaaaaaac
    54061 gatgggtcat cttggcctcc ttatctaatg atagtcacct taacagacaa gagtgtcaat
    54121 aatgtcattt taaggagaaa aagattaaaa atcaaggtca caaaacaatc tttaaataaa
    54181 aatgatgacc ctatatttat tgagtgagca cattttttgg atgatgtatg cttttatcaa
    54241 atagaggttt tttcatgaca attgctagca tttcggtgta aaataaaggt aataaccagc
    54301 aaaggagcaa cccatgacaa agcaagatca gttaatcgtt gaaaaaatgg aacaaaccta
    54361 tgaggccttt agcccaaagc tagcaaatct catagaagcc ctagatgcct ttaaagagca
    54421 ttatgaggaa tatgccacac ttagaaattt ttacagtagt gacgaatggt ttcggcttgc
    54481 aaaccaacct tgggatgata tcccgtgcgg tgtcctatct gaagacctcc tctttgatat
    54541 gatcggtgac cataaccaac tactagctga tatcttagac ttggccccta tcatgtacaa
    54601 gcatatgtaa ccatcgctaa cgtcacagat actacgatga caggacaaaa aaagctagcc
    54661 aatctcaaaa aaacagataa aaaacagata atcgttagaa aaacagtaga tgcgtctgtt
    54721 aaactaaagg tatcaaaaca aaaggagcat ttcatcatga aattcaaaaa agtattagtc
    54781 attcctgcat tagcattagc agcaacatgc tttttaacag cttgtggcac aaaaaaagat
    54841 tctaaaaaag aagaagttaa agaaatcaaa atgtctgaca tcaaagacga cgcagttagc
    54901 aaaaaaacaa aagtggttga tggcgaagaa gttacagaat acaccacaaa agatggaaac
    54961 gtgattcaga ttcctgcagg aaacgaagaa ggcatggaat caaaagatgc tggtggatca
    55021 ggcgctcctg ctaaaaacta agggttacaa caaagagcat aaggtgactc gcaaacgtta
    55081 cgcatgacat gaggcagtct gcctaagatg gtaactcact tatcataatg cctaaatagc
    55141 caaacacact aactcgcaaa tgtacaaaat catatacccg tgcttatgtg agcgaacggt
    55201 tatatgattt ttgttttcca aaaacaagaa aagaacgcta aaatagccag tttgtcgtgt
    55261 ttcaagctac caaaaaggaa gtgctaaaat gaaaaaagat ccattatacc tgtcatctta
    55321 ctgttggtca tcggattaat cgttggtgtt aagcgtcatc atgactagta ggtcttgcta
    55381 gtgaccgttc aaaagggata acgttggtat ggtataacca taaaaatcgt ataaaaagaa
    55441 aaaatagcta ttgaggagaa agtatgtttc agttaagaaa aaaaatgacg cgcaaacaat
    55501 tagccttgtt gagtgctgga gtgttgacct gtgtggttgg tggtagctac ttgataatga
    55561 accatcaaca acaagaaatt gtctctagtg tcaacaaagt aaaagcctta accataaaag
    55621 aagccatgga acaaggaaaa gatatcagct tgaccttagc tggcgaagta acagctaaca
    55681 acagcagcaa agtcaaaatc gactcaagta aaggagaagt caaagaggtc tttgttaaaa
    55741 aaggcgatgt tgtcaaagta ggacaaccct tgtttagcta tgaaacgtca cagcggttaa
    55801 cggctcaaag ttcagaattt gatgttcaaa ccaaagccaa tcagctccaa gttgctaaaa
    55861 ccaatgcagc attgaagtgg gaaacctaca atcgcaaggt caatgaaatc aacaccctaa
    55921 aatctcgcta caacactgca ccagatgaga gcttactaga gcaaattcgc agcgcagaag
    55981 acagtgtatc ccaagcacta agcgatgcca aaacagcaga tagcgatgtc aaaaccgctc
    56041 aaatcgaact cgataaagct aatgctactg ccacaacgga aaaaggtaaa ctagagtatg
    56101 acaccgttaa gtcagacacc gcaggaacca ttgttagtct aaatactgat ttgccaaatc
    56161 aatcaaaatc caaaaaagaa aatgaaactt ttatggaaat tatcgacaaa tcaaaaatgt
    56221 tagtcaaagg taacattagt gaatttgacc gtgacaagtt aaaaatcggt caaaaagtcg
    56281 aagtgattga ccgcaaagac aactctaaaa aatggactgg aaaagtaacc caagttggca
    56341 acctcaaagc agaggaaaaa ggccaaggtc aaggccaagg tggcaatgac caacaagata
    56401 atccaaacca agcaaaattc ccttatgtta ttgaacttga ccaatcagac aagcagccac
    56461 tcattggctc acacacctat gttaatgtgc tcaacaatgt tccagaagct ggcaagatcg
    56521 tattgaaaga aacctttaca atggcagaaa atggaaaaac ctatgtgtgg aaagttgata
    56581 aaaacaaggt caaaaaacaa gaaatcaaga ctaagccctt ctcaaaaggt tatgttgagg
    56641 taacaagtgg cttgactatg caagataaga ttgctcagcc gcttcctggc atgaaagacg
    56701 gtatggaggt aggaagtatt gttaaacctt aaagatattc gaaaaagcta tcatcttgga
    56761 actgaagaat ttgcgatttt aaaaggaatc gatttagaag ttaacgaggg tgacttttta
    56821 gccatcatgg gaccatcagg ttcgggaaag tcaacattga tgaatatcat tgggtgttta
    56881 gataagcctg gctctggctc atatgccatt gaaggcagag acgtgtcatc cttatctgat
    56941 aatgaacttg ctgatttgcg taatcaaaaa atcggctttg tttttcaaaa ttttaacctg
    57001 atgcccaagc taacagcttg tcaaaatgtc gaattaccct tgacttatat gaatgttcct
    57061 aaaaaagagc gtcgcaaacg agccctagag atgttaaagc tagtaggact agaagaacgt
    57121 agtgaattta aaccgatgga gctatctggt gggcaaaaac agcgtgtagc gattgcaaga
    57181 gctttagtca ctaacccgag ttttatcctt ggtgatgagc caacaggtgc actagacaca
    57241 aaaaccagcg tccaaatcat ggacctattt aaacaattca atgataacgg caaaacgatt
    57301 attatcatca cacacgagcc tgaagtagct gccctatgca aaaagacggt gatcctaaga
    57361 gatggtaata tagaacattc cgatatagag taaaggggga aaataatgga agatattatt
    57421 gcagctttaa gctctatatt atcacacaag atgcggtcaa ttttgacaat gctagggatt
    57481 attatcggta ttggtgccat tatcgcaatc ttttcaatca tcgaaggtaa taccgaaaat
    57541 accaaaagac agttgattgg tggcagcaat aacactatta acattgtgtt taacaaaaaa
    57601 agtagtattg accctaaatt tccagacaag tcaaatgcta aaaaaccaga ctatcttcct
    57661 tttatggctg aagaagagct tagcaaaata cagcaagtca aaggcgtcaa aaatgccctg
    57721 atctcatatg gtattgatga caaagtctac catctaggcc aaaaatcatc tgcaaagatt
    57781 tctgccatca caaaaaacgt agctgaagtg agacgcatga catttattaa aggcagtgac
    57841 ttttcagaca aagactttat tgaccaaaaa caggtgattt accttgaaaa aagcttgtat
    57901 gagtcactat ttccaaaaga cgatggcctt ggaaaatttg tcgaagtgat gggaaatccc
    57961 ttcagggtca ttggggtgtt tgaatcaaaa gaacaaagtg gcctcacaag tgggactgag
    58021 aaaattgctt atatcccgct acatcagtgg tataatatta atggtgtagt ggatgcgaca
    58081 cctgagatca cgatccagac ctatcgcgca gatgacctca aacctgtagc aaagcgtgtc
    58141 agtgatatgc tcaatcaaac gatccctaaa tcagattata tgtttggtgt catgaacctt
    58201 aaagaatttg aacgccaact agacaaccta aacaaatcta attttgtgtt attagctggt
    58261 attgctagta tctctcttat tgtaggtggt attggtgtga tgaacattat gctagtatca
    58321 gttaccgaaa gaacgagaga aattgggatc aaaaaagcgc ttggcgctag acgtaagctt
    58381 attttaaaac aatttttaat tgaagcggtc attctaacct tgctaggtgg tgtgataggg
    58441 gttatttcag ggatggtatc aggattgatc atcacaagat cactcgaata tccctatatc
    58501 ctatccttgt tctcagttgt cttgagttta gcgttttgtt gtattatagg gattgtcttt
    58561 ggactattac cagccattaa agcgtctaag ctagacccaa ttgaagcatt gcgatttgaa
    58621 taatcaacca aacaagcata aaagtcaaaa aggatgaccc tttgatgtca tccttagctg
    58681 cttgatacac taaacgccaa tctagaaagg gaagttatgt ttaaaatatt agtagttgaa
    58741 gatgatgata ccatcagtca agtgatttgt gagtttttaa aagcgaataa ctatgatcca
    58801 gattgtgtat ttgatggcca agctgcctta gacaagtggc agacaacgtc ttatgatctg
    58861 attattttag acatcatgct cccttcctta agtggcttag aagtgctaaa aacaattcga
    58921 aagacatcag atgtccctat tattatgcta acggcattag atgacgagta cacacagctt
    58981 gtaagcttca accatttgat tagtgattat gtgaccaaac cattttcacc actcatttta
    59041 atcaaacgta tcgaaaatgt attacgcgtc tctactccag atgaaaagcg ccaaatcgga
    59101 gacttacttg ttgatgagac cgaacatagc gtctattggc aaggaacatt ggtcaaacta
    59161 accaaaaaag aatatgacat tattgattat ttagcaaaac gtcaccaaaa aattgtcacc
    59221 agagaccaat tgatggatga catatggggc tattctgaac tggacacgcg tgtccttgac
    59281 aaccacatca agaaccttcg taaaaaaatg acgggtattc ccctcaagac aattacaggc
    59341 atgggctatt tattgggaga gcgtgagtga gactaatcaa aaaaaccttt ttggtcatca
    59401 atggcttaat tattgtagtg gttacctcta ttttgttagt gctttatttt gcaatgccaa
    59461 tttattacac caaagtcaaa gacaaagaag ttaagtgtga atttgaccaa acaagcaagc
    59521 aaatcaaagg caaaacggta accgagatta gagatatcct aacaaaaaaa atcaacaaag
    59581 ataatatttg gtacagctta gtagacagtg ataaccaact gctctatcct tccttgcagc
    59641 tcctagacgg cgtcagtgaa agcaaagaca gtcaaaatgt taatatcgtc accacctttg
    59701 acaactctta ttctaatgtt aaggtgatga gtcaaaaagt aaccctacgc gatggaaaaa
    59761 aaatgacatt gcttgggcaa tcttcacttc aacccgtaac agatgctagc aaagttctcc
    59821 ttgacctcta cccgtcacta ttgatttttt cagtgaccgt agggagtatt gtcgcctact
    59881 tatatagtag gacttctagc cggcgtatcc ttagcatgtc acaaaccgcc aaaaaaatgg
    59941 tcaacctaga accaaactta acgtgtacca ttcacggcaa agatgaaata gcaatgttgg
    60001 ctagtgatat taaccgtttg tatgcgagtc tttcaacgag catcaaatcc ttgcaaaaag
    60061 agtatgaaaa agcttctgat tcagaaagag agaaatctga gtttttacgc atgacatcgc
    60121 acgagctaaa gacaccaatc actagtgtta tcggaatgat tgatggaatg ctctataatg
    60181 ttggtgactt tgcagatcgt gacaaatatt tgcgaaagtg ccgagacgtc cttgaagggc
    60241 aagcccaact ggttcaatcc attttatctt tatctaagat tgaaacccta gcttctcaaa
    60301 atcaagaact gttttcacta aaaagcagcc tagaagaaga aatggaagtc tttctcgtct
    60361 tatcagaact aaaacacctc aaagtgacta tcaatcttga agaacaattc gtcaaagcca
    60421 ataaagtata tctactaaag gcgattaaaa atattattga caatgccttt cactatacca
    60481 aatcaggcgg ccaagtgatg attcaactaa aagacaacca actagtgatt aaaaatgaag
    60541 cagagacatt attgacacaa cagcagatga aacagttatt ccaaccgttt tatcgaccag
    60601 attatagtcg taacaggaaa gacggtggta caggattggg gctgtttatc acccaccaga
    60661 ttcttgatca gcatcatctg gcttatcgct ttgttgttct tgatcaaaga tggatggtat
    60721 ttacgattga ttttccatcc catcatgacg attgatccat actagtagga cgacaaaagt
    60781 catcaatcat taaccaagcc ttgaccttta cccatcattg tttaggcaag tataaaaaag
    60841 aggagagaaa tcatatatga agaaaaggaa attgttagca gtaacactat taagtaccat
    60901 actcttaaac agtgcagtgc cattagttgt tgctgatacc tccttgcgta atagcacatc
    60961 atccactgat cagcctacta cagcagatac tgatacggat gacgagagtg aaacaccaaa
    61021 aaaagacaaa aaaagcaagg aaacagcgtc gcagcacgac acccaaaaag accataagcc
    61081 atcacacact cacccaaccc ccccttcaaa tgatactaag cagaccgatc aggcatcatc
    61141 tgaagctact gacaaaccaa ataaagacaa aaacgacacc aagcaaccag acagcagtga
    61201 tcaatccacc ccatctccca aagaccagtc gtctcaaaaa gagtcacaaa acaaagacgg
    61261 ccgacctacc ccatcacctg atcagcaaaa agatcagaca cctgataaaa cacctgaaaa
    61321 aggaccagaa aaagcaactg ataaaacacc agagccaaat cgtgacgctc caaaacccat
    61381 ccaacctcct ttagcagctg ctcctgtctt tataccttgg agagaaagtg acaaagacct
    61441 gagcaagcta aaaccaagca gtcgctcatc agcggcttac gtgagacact ggacaggtga
    61501 ctctgcctac actcacaacc tgttgtcacg ccgttatggg attactgctg aacagctaga
    61561 tggttttttg aacagtctag gtattcacta tgataaagaa cgcttaaacg gaaagcgttt
    61621 attagaatgg gaaaaactaa caggactaga cgttcgagct atcgtagcta ttgcaatggc
    61681 agaaagctca ctaggtactc agggagttgc taaagaaaaa ggagccaata tgtttggtta
    61741 tggcgccttt gacttcaacc caaacaatgc caaaaaatac agcgatgagg ttgctattcg
    61801 tcacatggta gaagacacca tcattgccaa caaaaaccaa acctttgaaa gacaagacct
    61861 caaagcaaaa aaatggtcac taggccagtt ggataccttg attgatggtg gggtttactt
    61921 tacagataca agtggcagtg ggcaaagacg agcagatatc atgaccaaac tagaccaatg
    61981 gatagatgat catggaagca cacctgagat tccagaacat ctcaagataa cttccgggac
    62041 acaatttagc gaagtgcccg taggttataa aagaagtcag ccacaaaacg ttttgaccta
    62101 caagtcagag acctacagct ttggccaatg cacttggtac gcctataatc gtgtcaaaga
    62161 gctaggttat caagtcgaca ggtacatggg taacggtggc gactggcagc gcaagccagg
    62221 ttttgtgacc acccataaac ctaaagtggg ctatgtcgtc tcatttgcac caggccaagc
    62281 aggagcagat gcaacctatg gtcacgttgc tgttgtagag caaatcaaag aagatggttc
    62341 tatcttaatt tcagagtcaa atgttatggg actaggcacc atttcctatc ggacgttcac
    62401 agctgagcag gctagtttgt tgacctatgt cgtaggggac aaactcccaa gaccataacc
    62461 cccacaacca agaagctacc tgcctgttga ccaatctcaa aaagagacag cgtcaactta
    62521 gaatgaggtt catcagatga taaagaaagt gaccaccccg agtcaaaaaa caaaaaaacg
    62581 tgttcgtaat ggctacctcc taaaactagg tacagcctgt ttactcttaa gtatactcag
    62641 ttatggcatt ggtcttttgg gccagcctag catggaaaat acgtttatgg gaatagcgag
    62701 tgttgccatg ttaggaagtg tctgtttctt tattatcttt gctctaaacc gtatctttga
    62761 cgcattagag gacaatttgc gagattagag taataggtca aataatcttc caataattta
    62821 gagcacgagc taagttattg gaagtttttt ggtcttttga gagccatgat gtcatcaaaa
    62881 agccaaacac tccttatgat gtgagagtca catggctaaa attaaaagca aaatagaacc
    62941 caaagccctg ttttctcaaa atagccaacg aaaaaagaca agttaaccag ttcataaaaa
    63001 acatcgtaga aacgttttca agagctaatg ttggtgtaaa ttgactgaag tatgatagaa
    63061 tttttaatgg ttgtaccata acagtcaaac ttaaaaactg aaatcagtca aaaacattga
    63121 attgcatgta aaaaatctaa ttaagtt
//
LOCUS       DAXWIQ010000004        61111 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000004 DAXWIQ010000000
VERSION     DAXWIQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 61111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61111
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.004"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(302..1639)
                     /gene="pepC"
                     /locus_tag="ACGEUI_000219"
     CDS             complement(302..1639)
                     /gene="pepC"
                     /locus_tag="ACGEUI_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HFF8165088.1"
                     /translation="MSALTETFTEQLFAHYEANAKFSAIENAVTHNGLLKSLETRQSE
                     VDNDFVFSIDLTKDKVSNQKASGRCWMFAALNTFRHKLITEFKLENFELSQAHTFFWD
                     KYEKANWFMEQVIATADQELTSRKVKFLLDVPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESISSSNSRELNQYLNKLLRQDAQILRDLIASGAKADQVEDRKAELLQEIFNFLAMTL
                     GLPPRHFDFAYRDKDDHYHVEKGLTPQAFYDKFVGLKLSDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSREVRYLNLDMKRFKELAIKQMQAGESVWFGSDVGQVSDRQKGILATN
                     TYDFEASMDINLSQDKAGRLDYSESLMTHAMVLTGVDLDETGKPLKWKVENSWGEKVG
                     DKGYFVASDAWMDEYTYQIVVRKEFLTADELAAYEKEPQVLAPWDPMGALAQY"
     gene            complement(1824..2648)
                     /gene="nadE"
                     /locus_tag="ACGEUI_000220"
     CDS             complement(1824..2648)
                     /gene="nadE"
                     /locus_tag="ACGEUI_000220"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFF8165089.1"
                     /translation="MTLQEEIIRQLGVKASIDPQEEIRKAVDFLKAYLRKHSFLKTYV
                     LGISGGQDSTLAGKLAQMAIAELREEASDQAYQFIAVRLPYGVQADEADAQKALAFIA
                     PDQTLTINIKAAVDGQVEALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGT
                     DHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQK
                     PGLADEVALGVTYQDIDDYLEGKLISKVAQATIEKWWHKGQHKRHLPITIFDDFWK"
     gene            complement(2650..4104)
                     /locus_tag="ACGEUI_000221"
     CDS             complement(2650..4104)
                     /locus_tag="ACGEUI_000221"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFF8165090.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFEQGIHNRHAVFEVYFRKEPFN
                     NGYAVFAGLQRMVEYLEQFQFSETDLAYLEELGYPENFLTYLKELRLELTIRSAKEGD
                     LVFANEPIVQVEGPLGQCQLVETALLNIVNFQTLIATKAARIRSVIEDEPLLEFGTRR
                     AQELDAAIWGTRAAMIGGADATSNVRAGKRFDIPVSGTHAHALVQAYGNDYDAFMAYA
                     KTHKDCVFLVDTYDTLKVGVPTAIRVAKEMGDKINFLGVRLDSGDLAYLSKTVRQQLD
                     DAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     QEDGSMRDTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITFTDINVNELTEIEM
                     FHPTYTYIKKTVKEFDAIPLLVDIFVKGELVYQLPTLAEIKAYAKKEFDKLWDEYKRV
                     LNPQDYPVDLARDVWQNKMALIDNIRKDAYGKSE"
     gene            complement(4275..5654)
                     /locus_tag="ACGEUI_000222"
     CDS             complement(4275..5654)
                     /locus_tag="ACGEUI_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8165091.1"
                     /translation="MSIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SIALTGPSIIFVYMITGAFMFMMMRAIGEMLYYDPDQHTFINFISKYIGPGWGYFSGL
                     SYWISLIFIGMAEITAVGAYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFW
                     FAMIKILAILALIATAIFMVLTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVF
                     FAYQAIEFVGITTSETANPRKVLPKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDE
                     SPFVMVFKLIGIKWAAALINFVVLTSAASALNSTLYSTGRHLYQIANETPNALTNRLK
                     INTLSRQGVPSRAIIASAVVVGISALINILPGVADAFSLITASSSGVYIAIYALTMIA
                     HWKYRQSKDFMADGYLMPKYKVTTPLTLAFFAFVFISLFLQESTYIGAIGATIWIIVF
                     GIYSNVKFK"
     gene            complement(5823..6740)
                     /gene="trxB"
                     /locus_tag="ACGEUI_000223"
     CDS             complement(5823..6740)
                     /gene="trxB"
                     /locus_tag="ACGEUI_000223"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFF8165092.1"
                     /translation="MYDTLIIGSGPAGMTAALYAARSNLSVAIIEQGAPGGQMNNTFD
                     IENYPGYDHISGPELAMKMYEPLEKFNVENIYGIVQKIENFGDYKCVLTEDASYEAKT
                     VIIATGAKYRVLGVPGEEYYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAIYLT
                     QFAKKVTVVHRRDQLRAQKILQDRAFANDKVDFIWDSVVKEIQGNDIKVSNVLIENVK
                     TGQVTDHAFGGVFIYVGMNPVTGMVKDLEITDSEGWIITDDHMRTSIPGIFAIGDVRQ
                     KDLRQITTAVGDGAIAGQGVYHYLESFSS"
     gene            complement(6804..7028)
                     /locus_tag="ACGEUI_000224"
     CDS             complement(6804..7028)
                     /locus_tag="ACGEUI_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HFF8165093.1"
                     /translation="MLVEIFSLYVKGLILSSLTMILICLIWTLWRAKTKRDKTLIERQ
                     AFLYEMLMVAILTIPILSFAFMSILVVIKS"
     gene            complement(7132..7875)
                     /locus_tag="ACGEUI_000225"
     CDS             complement(7132..7875)
                     /locus_tag="ACGEUI_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8165094.1"
                     /translation="MITIRNLSKTFSGQKVLDSLALDIEKGQVIALVGASGAGKSTFL
                     RSLNYLEKPDSGSISIGDFTVDFETITTEQVLILRRKLAMVFQQFNLFERRTALENVK
                     EGLKVVKKLSDQEATKLAQAELAKVGLADRKHHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSITDAAKSGQTMVLVSHDMNFVYQVADRVLFLDQGKIL
                     EQGTPEEVFRHPQKERTKEFFASYSKTYI"
     gene            complement(7875..8678)
                     /locus_tag="ACGEUI_000226"
     CDS             complement(7875..8678)
                     /locus_tag="ACGEUI_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8165095.1"
                     /translation="MTSVFLTSGWAFYDYLISPIPHGKLFSWHAVFDAIPNIIQRLPI
                     TLGLTLSGATFGLVLALIFALVKINKVKLLYPIQAIFVSFLRGTPILVQLMLTYYGIP
                     LFLKFLNQKYGFDWNVNAIPASIFAITAFAFNEAAYASETIRAAILSVDTGEIEAAKS
                     LGMTSVQVYRRVIIPNATVVAIPTLINGLIGLTKGTSLAFNAGIVEMFAQAQILGGSD
                     YRYFERYISVALVYWSISILMEQVGRLIENKMAIKAPEQARNEKLGELR"
     gene            complement(8873..10216)
                     /locus_tag="ACGEUI_000227"
     CDS             complement(8873..10216)
                     /locus_tag="ACGEUI_000227"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8165096.1"
                     /translation="MSFKDYHFKQYVQQALEEIGFVNPTEVQKRLIPIVNSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDEAKAEVQVVITAPSRELATQIFDACKQIAKHFQEEIRL
                     ANYVGGTDKLRQIEKLKDSQPHIVIGTPGRIYDLVKSGDLAIHKATTFVVDEADMTMD
                     MGFLDTVDKIAASLPKSVQILVFSATIPQKLQPFLKKYLTNPVIEQIKTKTVIADTID
                     NWLVSTKGRDKNGQLLEILKTMQPYMAMLFVNTKERADDLHAFLTANGLKVAKIHGGI
                     PPRERKRIMNQVKKLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGMAGTAITLYQPSDDSDIKELEKMGIAFTPKVLKNGEFQDTYDRDRRQNREKAYQK
                     LDTEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKERRAENRAKGRAERKAKKQHF"
     gene            complement(10374..11384)
                     /gene="mraY"
                     /locus_tag="ACGEUI_000228"
     CDS             complement(10374..11384)
                     /gene="mraY"
                     /locus_tag="ACGEUI_000228"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFF8165097.1"
                     /translation="MFLTLIAAIISFMVSAFTMPYFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLLVATAVSLLVSLFSIKNTQSLALISGILSIVVIYGIIGFLDDFL
                     KIFKQINEGLTAKQKLALQLVGGLMFYFLHVSPSGISSINVFGYQLPLGIFYLFFVLF
                     WVVGFSNAVNLTDGIDGLASISVVISLVTYGVIAYVQSQFDVLLLIGAMIGALLGFFC
                     FNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLIIGIVYVLETSSVMLQVSYFKY
                     TKKKYGEGRRIFRMTPFHHHLELGGLSGKGKKWSEWQVDAFLWGVGSLASLLVLAILY
                     VF"
     gene            complement(11386..13641)
                     /gene="pbp2X"
                     /locus_tag="ACGEUI_000229"
     CDS             complement(11386..13641)
                     /gene="pbp2X"
                     /locus_tag="ACGEUI_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2X"
                     /protein_id="HFF8165098.1"
                     /translation="MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIIN
                     FMIIIGTDQKFGVSLSEGAKKVYQETVTIQAKRGTIYDRNGTAIAVDSTTYSIYAILD
                     KSFVSASDEKLYVQPSQYETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGIS
                     YSTMSTIQKAMEDAKIKGIAFTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGK
                     TGLEASFDKILSGQDGVITYQKDRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLE
                     TQMDVFQAKSNGQLASATLVNAKTGEILATTQRPTYNADTLKGLENTNYKWYSALHQG
                     NFEPGSTMKVMTLAAAIDDKVFNPNETFSNANGLTIADATIQDWSINEGISTGQYMNY
                     AQGFAFSSNVGMTKLEQKMGNAKWMNYLTKFRFGFPTRFGLKDEDAGIFPSDNIVTQA
                     MSAFGQGISVTQIQMLRAFTAISNNGEMLEPQFISQIYDPNTASFRTANKEIVGKPVS
                     KKAASETRQYMIGVGTDPEFGTLYSKTFGPIIKVGDLPVAVKSGTAQIGSEDGSGYQD
                     GGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFGPLFWQDVVNPVLEEAYLMQDTLTK
                     PVVSDANRQTTYKLPNFVGKNPGETSSELRRNLVQPVVLGTGSKIKKVSHQPGQTLTE
                     NQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISFKGTDSGRVMKQSVDVGKSLK
                     KIKKMTITLGD"
     gene            complement(13645..13968)
                     /gene="ftsL"
                     /locus_tag="ACGEUI_000230"
     CDS             complement(13645..13968)
                     /gene="ftsL"
                     /locus_tag="ACGEUI_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFF8165099.1"
                     /translation="MTNEKRTQAVTNALQKRIKTFSRIEKAFYTAIIVTAITMAVSII
                     YLQSRKLQLQQEITSLNSHISDQKLELNNAKQEVNELSRRDRIIDIAGKAGLSNRNNN
                     IKKVE"
     gene            complement(13973..14923)
                     /gene="rsmH"
                     /locus_tag="ACGEUI_000231"
     CDS             complement(13973..14923)
                     /gene="rsmH"
                     /locus_tag="ACGEUI_000231"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFF8165100.1"
                     /translation="MTKEFHHVTVLLHETVDMLDIKPDGIYVDATLGGSGHSAYLLSK
                     LGEEGHLYCFDQDQKAIDNAQVTLKSYIDKGQVTFIKDNFRHLKARLTALGVDEIDGI
                     LYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLTAYEVVNTYPFNDLVKIFFKYG
                     EDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQDAMELLALDGRISVITFHSLEDRLTKQLFKEASTVDVPKGLPLIPE
                     DMKPKFELVSRKPILPSHSELTANKRAHSAKLRVAKKIRK"
     gene            complement(15300..15440)
                     /locus_tag="ACGEUI_000232"
     CDS             complement(15300..15440)
                     /locus_tag="ACGEUI_000232"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165101.1"
                     /translation="MTNEREKEEAKVTLEELLHQIQVIHLELKIVRNWGYFVFFWRWY
                     AG"
     gene            complement(15460..16710)
                     /locus_tag="ACGEUI_000233"
     CDS             complement(15460..16710)
                     /locus_tag="ACGEUI_000233"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFF8165102.1"
                     /translation="MTDMRRLGQRAKQASLLIAPLSTQIKNRFLSTLAKALVDDTQTL
                     LAANQKDLANAKEHGISDIMMDRLRLTSERIKAIAQGVQQVADLADPIGQVIKGYTNL
                     DGLKILQKRVPLGVIAMIFESRPNVSVDAFSLAFKTNNAIILRGGKDALHSNKALVKL
                     IRQSLEKSGITPDAVQLVEDPSHAVAEELMQATDYVDVLIPRGGAKLIQTVKEKAKVP
                     VIETGVGNVHIYVDAQADLDIATKIVINAKTKRPSVCNAAEGLVIHEAVAARFIPMLE
                     KAINQVQPVEWRADDKALPLFEQAVPAKAEDFETEFLDYIMSVKVVSSLEEAISWINQ
                     YTSHHSEAIITRDIKAAETFQDLVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKMHA
                     RGPMGLEALTSTKFYINGDGHIRE"
     gene            complement(16703..17524)
                     /gene="proB"
                     /locus_tag="ACGEUI_000234"
     CDS             complement(16703..17524)
                     /gene="proB"
                     /locus_tag="ACGEUI_000234"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFF8165103.1"
                     /translation="MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLM
                     NKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAVSSVGQVAMMSLYSQIFAYYQTN
                     VSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
                     LSAVVAGITKADLLIMLSDIDGLFDKNPTIYEDAQLRSHVANITQEIIASAGGAGSKF
                     GTGGMLSKVQSAQMVFENKGQMVLMNGANPRDILRVLEGQPLGTWFKQIEEVRHD"
     gene            complement(17589..19217)
                     /locus_tag="ACGEUI_000235"
     CDS             complement(17589..19217)
                     /locus_tag="ACGEUI_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8165104.1"
                     /translation="MNWSTIWELIKINILYSNPQSLANLKKRQEKHPKENFKAYKSMI
                     RQQALMIAMFLVIYLFMFIGVDFSHYPGLFSFDVAMFFIMSTLTAFSSLYTIFYESND
                     LKLYIHLPVTSEELYIAKIVSSLGMGAVFLMPLISLLLIAYWQLLGNPLSILVAIVLF
                     LVLLVSSMVLAIYINAWVGKIIVRSRKRKLISTIMMFVSTFGAFVLIFAINISNNKRT
                     MTDGVFTDYPTIPYFKGFYDVVQAPFSTAALLNFWLPLLLILAMVYGIVTKVMPTYYR
                     EAFYISNENKVKQTKKPVNRPHQNQSLAQLLRKHHLLTLQNATLLTQTYLMPLMYVML
                     FIGPSLSRGTGFFKHISPDYFGVALLFGVSLGVMCATPTSFIGVGISLEKDNFTFIKS
                     LPITLKKFLMDKFCLLVGLQLIVPMVIYLVFGLFVLHLHPLLTIAFCLGYALSLIVQG
                     ELMYRRDYRLLDLKWQDMTQLFTRGDGQWLTMGLIFGNLIVAGVLGFGAVIIANIIQQ
                     PLLISILLSCLILMVLGLAQLWIQKTFWKSLERL"
     gene            complement(19222..19956)
                     /locus_tag="ACGEUI_000236"
     CDS             complement(19222..19956)
                     /locus_tag="ACGEUI_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8165105.1"
                     /translation="MIEFKHVSKLYGDKEALSDLNVTINDGEIFGLIGHNGAGKTTTI
                     SILTSIIEASYGEVFVDGQLLTENREAIKKQIAYVPDSPDIFLNLTPNEYWQFLAKIY
                     GVSDEDREERLAQLTTLFELKEEVNQTIDSFSHGMRQKVIVIGALVSNPNIWILDEPL
                     TGLDPQASFDLKEMMKAHAASGHTVLFSTHVLSVAEQLCDRIGILKKGKLIFVGTIDE
                     LKEHHPDKDLESIYLELAGRKAQEEG"
     gene            complement(19990..20256)
                     /locus_tag="ACGEUI_000237"
     CDS             complement(19990..20256)
                     /locus_tag="ACGEUI_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFF8165106.1"
                     /translation="MEKKRQRLYDVIRQAYDYPENRENVALSQLLLAASNRLIKHSNP
                     LLVAYQLNQDVDNYLLDNDILLPKSLCRFKQSLEKYILEEASLV"
     gene            complement(20449..22434)
                     /gene="tkt"
                     /locus_tag="ACGEUI_000238"
     CDS             complement(20449..22434)
                     /gene="tkt"
                     /locus_tag="ACGEUI_000238"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFF8165107.1"
                     /translation="MTFDAIDQLAVNTVRTLSMDAIQAANSGHPGLPMGAAPMAYVLW
                     NHFMNINPKTSRNWSNRDRFILSAGHGSAMLYSLLHLAGYDLSVEDLKNFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDIVDHYTFALNGDG
                     DLMEGVSQEAASMAGHLKLGKLVLLYDSNDISLDGPTSMAFTEDVKGRFEAYGWQHIL
                     VKDGNDLEEIAAAIEAAKAETEKPTIIEVKTIIGFGAEKQGTSAVHGAPLGAEGIAFA
                     KKAYQWTHQDFEVPAEVTERFAQGLQARGEKAEQAWNDLFAAYEAEYPELAAEYQKAF
                     TNEAAQVELEAHELGSSMASRVSSQQAIQQISEQVASFWGGSADLSASNNTMVKAETD
                     FQPGHYEGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWKRAIAET
                     DRPTMLVLTRQNLPVLEGTKELAEDGLSKGAYILSEAKGDLDGILIATGSEVKLAMDT
                     QEALEAEGIHVRVVSMPSQNIFDEQSAEYKESILPAAVTKRLAIEAGSSFGWAKYVGL
                     SGKTLTIDTWGASAPGNRIFEEYGFTVANATELYKSL"
     gene            complement(22652..23296)
                     /gene="fsa"
                     /locus_tag="ACGEUI_000239"
     CDS             complement(22652..23296)
                     /gene="fsa"
                     /locus_tag="ACGEUI_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFF8165108.1"
                     /translation="MKFFLDTANVAAIKAINELGVVDGVTTNPTIISREGRDFETVIK
                     EICDIVDGPISAEVTGLTADAMVEEARSIAKWHDNVVVKIPMTTEGLKATNILSKEGI
                     KTNVTLIFTVSQGLMAMKAGATYISPFIGRLEDIGTDAYQLISDLREIIDLYDFQAEI
                     IAASIRTTAHVEAVAKLGAHIATIPDPLFAKMTQHPLTTNGLKTFMEDWASFKK"
     gene            complement(23422..24921)
                     /locus_tag="ACGEUI_000240"
     CDS             complement(23422..24921)
                     /locus_tag="ACGEUI_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8165109.1"
                     /translation="MKIEDLMDKERRAQYRLLVTLYHAKETLRLKDLMRLSNLSKVTL
                     LKYIDNLNHLCREQGLACQLLLEKDSLSLKENGQFHWEDLVALLLKESVAYQILTYMY
                     CHEHFNITNLSVELMVSEATLNRQLAHLNQLLSEFDLALSQGRQLGSELQWRYFYFEL
                     FRHTLTRQGIDALVNQLDASHLATLIERLIGQSLSAEALEQLLIWLAISQARMSFQKG
                     YNDHFLRDSDFMTSNIFFKRLESMLLHYLRRYALEFDAFEAKSLFVFLHAYPLLPIAS
                     MKYSLGFGGPIADHISEALWLLKKAHVIIHQTKEEIIYGLGIFFSKAYFFKGAILSQP
                     TNSQYLYQLVGEDKRALLRVIINHLVLQMDQETDFSQQLSDDVLALLIFSIERHHEPL
                     LVGLALGQNKVEAAIAELAIRRHLGHRRDFQLMPYDHQKVYDCLITYQTVCLPRQDLP
                     YYRLKQYSSPYELTALEAFLKDLFQQKNTREEELSLSPAAKSSFAHKTV"
     gene            complement(24911..26257)
                     /locus_tag="ACGEUI_000241"
     CDS             complement(24911..26257)
                     /locus_tag="ACGEUI_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8165110.1"
                     /translation="MTLTTKTIHVIGASFAGLAFVDKYKDLNPDSQIILIDKESCPNY
                     IPNGINQLFRGDIQDLSDAMWGRACLAAQIESNHRFIQAEVLAIEAPSNTLLLKDSQG
                     RVFEEGYETLVCAMGASPQSHYIETSQTNKVLVTKYYEESQASLKLIEASQEVLVIGA
                     GLIGLDLAYSLSLQGKRVKLIEAAERPDFYQTDAELIAPVMAEMSTHHVTFINNKRVT
                     AIHEIEGKVVAHTEQGDTFQGDLAILAINFRPNTHLLQGQVACALDKTILVNENLQTS
                     QANIYAIGDMVSLHFGILGMDYYTPLINQAMKTGQALALHLAGYPIPPLQTVKVLGSS
                     HFDYYRASVGVTEEEAELYMDTCSYLYQNGDSKNLFWLKLIARKTDGILIGAQLLSKT
                     NALVIANQLGQALALKVTDADLAFQDFLFLQGHSDLAYHLHEACLKLFEKRLRHED"
     gene            complement(26366..27067)
                     /locus_tag="ACGEUI_000242"
     CDS             complement(26366..27067)
                     /locus_tag="ACGEUI_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFF8165111.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWG
                     IAVAVAAFISGKVAPAHLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMVGSTLVFLQ
                     FRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
                     TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGP
                     IIGAVLAVLLFQVMS"
     gene            complement(27069..28907)
                     /gene="glpO"
                     /locus_tag="ACGEUI_000243"
     CDS             complement(27069..28907)
                     /gene="glpO"
                     /locus_tag="ACGEUI_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFF8165112.1"
                     /translation="MEFSRETRRLALQKMQERDLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAQGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVSNTPAANKVLTKEEVLKREPDLKQEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGALIASHVKAEDFLLDDNGKIIGVKARDLLS
                     DQEIIIKAKLVINTTGPWSDEIRQFSHKGQPIHQMRPTKGVHLVVDRQKLPVSQPVYV
                     DTGLNDGRMVFVLPREEKTYFGTTDTDYTGDLEHPQVTQEDVDYLLGVVNNRFPNANV
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKVSDDSFDHLVDTVKAYINHEDSREAVE
                     KAIKQVETSTSEKELDPSAVSRGSSFERDENGLFTLAGGKITDYRKMAEGALTGIIQI
                     LKEEFGKSFKLINSKTYPVSGGEINPANVDSEIEAYAQLGTLSGLSMDDARYLANLYG
                     SNAPKVFALTRQLTAAEGLSLAETLSLHYAMDYEMALKPTDYFLRRTNHLLFMRDSLD
                     ALIDPVINEMAKHFEWSDQERVAQEDDLRRVIADNDLSALKGHQEG"
     gene            complement(28923..30449)
                     /gene="glpK"
                     /locus_tag="ACGEUI_000244"
     CDS             complement(28923..30449)
                     /gene="glpK"
                     /locus_tag="ACGEUI_000244"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFF8165113.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAG
                     WVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGITNQRETTVVWDKKTGVPIYNAI
                     VWQSRQTAPIAEQLKQDGHTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAVHVTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEV
                     KSNSEIYGKTAAFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GDEMQLSSNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLKMIETSPESEQFA
                     LASTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRDV
                     IDTMQVDSGIDIQQLRVDGGAAMNNMLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     AVGYWEDMDALKELNATGQLFKASMNESRKEKLYKGWKRAVKATQVFTQEEDADDDAK
                     "
     gene            complement(30810..31202)
                     /locus_tag="ACGEUI_000245"
     CDS             complement(30810..31202)
                     /locus_tag="ACGEUI_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFF8165114.1"
                     /translation="MCLSYNRRNQRKKVVLMTIKKFSLLAIASLSLLSLAACDMDDKD
                     DHMDNQPKTSQTSKKVKLSEDKAKSIALKDASVTEADAQMLSVTQDNEDGKAVYEIEF
                     QNKDQEYSYTIDANSGDIVEKSSEPIND"
     gene            complement(31329..31586)
                     /locus_tag="ACGEUI_000246"
     CDS             complement(31329..31586)
                     /locus_tag="ACGEUI_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFF8165115.1"
                     /translation="MDPKKIARINELAKKKKTVGLTGPEKVEQAKLREEYIEGYRRSV
                     RHHIEGIKLVDEEGNDVTPEKLRQVQREKGLHGRSLDDPKS"
     gene            complement(31740..33779)
                     /gene="glyS"
                     /locus_tag="ACGEUI_000247"
     CDS             complement(31740..33779)
                     /gene="glyS"
                     /locus_tag="ACGEUI_000247"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFF8165116.1"
                     /translation="MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDI
                     QTFSTPRRLAVRVSGLADQQTDLTEDFKGPAKKIALDADGNFSKAAQGFVRGKGLTTD
                     AIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVH
                     TLTVLLNDEALELDFLDIHSGRVSRGHRFLGTETTITSADSYEADLRSQFVIADAKER
                     QEMIVEQIKTLEVEQGVQVDIDEDLLNEVLNLVEFPTAFMGSFEAKYLDVPEEVLVTS
                     MKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLVARLEDGEFFWREDQ
                     KLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIY
                     KFDLLTGMVGEFDELQGIMGEKYALLAGEDAAVATAIREHYLPDAAGGALPETKVGAV
                     LALAAKLDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILDHFGWRIPMDKLVDSLYD
                     LSFDSLTYANKADVMNFIRARVDKMMGKAAPKDIREAILASSTFVVPEMLAAAEALVK
                     ASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAAIQGLTLAGSAAQ
                     QLEQVFALSPVINDFFDNTMVMAGDQALKNNRLAILSDLVSKAKTIVAFNQLNTK"
     gene            complement(34157..35074)
                     /gene="glyQ"
                     /locus_tag="ACGEUI_000248"
     CDS             complement(34157..35074)
                     /gene="glyQ"
                     /locus_tag="ACGEUI_000248"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFF8165117.1"
                     /translation="MSKKLTFQEIILTLQQYWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLASLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFLQPEYEHSKYSFEISDQDMLLENFE
                     KFEKEASRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEATRAILLAEDDE"
     gene            complement(35446..35979)
                     /locus_tag="ACGEUI_000249"
     CDS             complement(35446..35979)
                     /locus_tag="ACGEUI_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFF8165118.1"
                     /translation="MTIFLVMLAMFTIRLVFLKKSITNEKAILAQGGQEFGAQNTKFL
                     TLLHIMIYVFAVIEALLKQIKFDGISFLGLLLMLLSVAVLYEVTRILGDIWTVKLMLA
                     KDHKYVDHWLFKTIKHPNYFLNIAPELVGIALLCHAKITAMLLFPCYIVVIYLRIREE
                     NKLLAEVIIPNGTRTKP"
     gene            complement(36111..36950)
                     /locus_tag="ACGEUI_000250"
     CDS             complement(36111..36950)
                     /locus_tag="ACGEUI_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFF8165119.1"
                     /translation="MVTTVKMTSGYEIPVLGFGTYQAADGEEAYQSTLAAIKAGYRHI
                     DTAAIYKNEESVGRAIKDSGVLREDLFITTKLWNDAHSYEGAKDALAASLDRLGLDYV
                     DLYLIHWPNPKALRNTWKEANAQAWQYMEEAVEAGLIKSIGVSNFMVHHLEALQETAK
                     ITPAINQIRLAPGCYQKEVVDYCKANEILLEAWSPLGQGEIFDNETMQQLANKYDKTV
                     AQVALAWSLAEGFIPLPKSVHDERIKENMAIFDVSLTQEDKKTIRYLSGMSAIPTPDT
                     TSF"
     gene            complement(37072..38220)
                     /gene="nagA"
                     /locus_tag="ACGEUI_000251"
     CDS             complement(37072..38220)
                     /gene="nagA"
                     /locus_tag="ACGEUI_000251"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFF8165120.1"
                     /translation="MTCYLKADCFYYPTEVRPAGYLSLHDGVFGEWTEIVPADAQIID
                     YTGYQIAPGLVDTHIHGYAGADVMDNSAQGIHQMSEGLLATGVTSFLPTTLTSTFEQL
                     EKVSGTIASVADQVKGAKIQGIYFEGPYFTEEYKGAQNPSYMKTPRLEEFDAWQKAAK
                     GLIKKIALAPERDGVKEFVSAVTKQGVTVALGHSNGTYQEAKEAVQAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVSPIACDILMQQKGHDHVAMITDCMRA
                     GGSPDGDYLLGEFSVVVANGTARLKESGNLAGSILKLKDGLRNVVAWGIATPAEAIHM
                     ATYVPAVSVGIDDVCGQIKAGHAADFIVLDKDLTLVATYLNGQKAFDA"
     gene            complement(38338..39969)
                     /locus_tag="ACGEUI_000252"
     CDS             complement(38338..39969)
                     /locus_tag="ACGEUI_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFF8165121.1"
                     /translation="MSVNWQDIAFHFFGGLGLFLFSIKYMGDGLQQAAGDKLRYYIDK
                     YTSNPFFGILVGIAMSALIQSSSGVTVITVGLVSAGLLNLRQAIGIVMGANIGTTITS
                     FLIGFKLGDYALPMIFIGAACLFFTSNKKLNNFGRIIFGVGGIFFSLNLMGDAMDPLK
                     SVSAFQNYLATLGDKPFQGVFIGTALTMLIQSSAAIIGILQGLFSGGLLTLQGAIPIL
                     LGSNIGTCITAVLAAIGSNIAAKRVAAAHVLFNLIGTIIFMIILVPFTSLMLWLQSKL
                     SLTPEMTIAFSHGSFNITNTILLIPFISLLAMIVTRLIPGEDEVVKYEALYLDRLLIT
                     QAPSIALGNAHKELVHLASYAIQAFEASYSYIMTADGKFGEKVKRYERAVDTIDEELT
                     TYLVDISNEALSPSENEVLAGILDSSRDLERIGDHSESLGILIEGIISKQIGFSISAR
                     QELTEMYQLTHCLTLDAIRAIVDSDTDLAQTIVTRHKEIEEKERRLRKTHIKRLNCGE
                     CTAQAGINFIDIISHYTRITDHALNLAEKVLSHQL"
     gene            40171..40893
                     /locus_tag="ACGEUI_000253"
     CDS             40171..40893
                     /locus_tag="ACGEUI_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165122.1"
                     /translation="MATMKQYHKGDSMTKSQKEALYWMLSVLTITLIGGSCLIFGSHP
                     QTQDKVAKHSKSAASLLKKAVKAVNDADRLATAAAIQEAQKAVDKLAESSKKKTLQEQ
                     LNVAKAKQEQEDAATQAVKAAEETLNQNLKDIAQKAVNDLSNKGKKAALQSRLDAILP
                     AKPIIDEFPRQSGEITDNSYWTPFPGDVSDTYDNSQSPTLDPSSESSASDVTPQPSHP
                     DPIPPQTSSEPSDSGDKQSSKE"
     gene            complement(41022..41864)
                     /locus_tag="ACGEUI_000254"
     CDS             complement(41022..41864)
                     /locus_tag="ACGEUI_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8165123.1"
                     /translation="MTWKIVTDSGCDLRSLTRQSKELRFERVPLTLQIGTEIFRDDDG
                     LDIDNMMTTMYQSSKATTSSCPSPEAFLQAYRGADNVIVMTITGTLSGSHNSARLAKN
                     ELLEENPNVNIHLIDSLSAGGEMDLLVLELERLIKLGLSFEEVVKQITAYQQKTRLIF
                     VLAKVDNLVKNGRLSKLVGKVIGLLNIRMVGKASNKGTLELLQKARGQKKAVSALIEE
                     IQKEGYVGGKVYIAHAQNPKICEQISEKIKSLYPDAVIQTGRTSGLCSFYAEDGGLLM
                     GYEI"
     gene            42157..42714
                     /locus_tag="ACGEUI_000255"
     CDS             42157..42714
                     /locus_tag="ACGEUI_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8165124.1"
                     /translation="MTDKALSKRSLQNLTQFNKETRRIARESMEIALLNLLETKPLGD
                     ITISELVTKAGVSRNAFYRNYTSKEAIIEQLLVGVIRRIFRGLKQFDIKTQAYQAWLY
                     LFTEAKKEAQLLKMIFKHHLHHLLTRLVTKRLKAYQKLKDKKQSHYKRLFWSNAIVSV
                     LTNWISDDMIVPAEEMAAMGLPLLT"
     gene            complement(42750..43574)
                     /locus_tag="ACGEUI_000256"
     CDS             complement(42750..43574)
                     /locus_tag="ACGEUI_000256"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8165125.1"
                     /translation="MIQLIAIDLDGTLLNQDKQIPKENITAIQEAAQSGLKIVLCTGR
                     PQSGTRPYFDQLGLTQEEFLIINNGCSTYSSPDWQLRHSKMLKVSDIELLEELSQSFP
                     DIYLTLTEENDYLVLEEEVPDLVQEDGDLVFTIVKPVSLAELSDTPRLIFQAMYLGEK
                     AALDAFERAVRNQLSQSFHVVRSQDNILEILPQGVSKASALKELVEDLGLTADQVMAI
                     GDAPNDIEMLTYAGLGVAMENASAAIKPLADKVTLTNDMAGVAQAIRQFALVAQKD"
     gene            complement(43576..44193)
                     /locus_tag="ACGEUI_000257"
     CDS             complement(43576..44193)
                     /locus_tag="ACGEUI_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFF8165126.1"
                     /translation="MTIDMWEILNIIGTIAFALSGAIVAMEEEFDILGIFILGFVTAF
                     GGGAIRNTLIGLPIEALWGQKPEFTCAFFAMVLIMLFPKLMARGWVRAAVLTDAIGLA
                     AFSVQGALHAVRLNQPLSAVIVAAVLTGAGGGVVRDILAGRKPSVLRSEIYAGWSILA
                     AIVLHFKLADSTIECYALVVLLTTLRMIGNRKKWNLPKIKWKEER"
     gene            complement(44390..45367)
                     /gene="lacD"
                     /locus_tag="ACGEUI_000258"
     CDS             complement(44390..45367)
                     /gene="lacD"
                     /locus_tag="ACGEUI_000258"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8165127.1"
                     /translation="MTITANKRHYLEKVSHQGIISALAFDQRGALKQMMAAHQEGEAT
                     VTQIETLKVLVSEELTPYASSILLDPEYGLLATKVRANQTGLLLAYEKTGYDATTTSR
                     LPDCLVEWSVKRLKAAGADAIKFLLYYDVDGDEQINLQKQAYIERIGSECTAEDIPFF
                     LELLSYDERISDNNSAAYAKLKPHKVNGAMSVFSDKRFGVDVLKVEVPVNMAYVEGFT
                     EGEVHYSQAEAIKAFQDQEAASHLPYIYLSAGVSAKLFQETLYFAAAAGAQFSGVLCG
                     RATWAGSVPVYITKGEDEARKWLCTEGFQNIDELNRVLEETASPWTEKI"
     gene            complement(45414..45867)
                     /locus_tag="ACGEUI_000259"
                     /pseudo
     CDS             complement(45414..45867)
                     /locus_tag="ACGEUI_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061423.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
     gene            complement(45877..46392)
                     /gene="lacB"
                     /locus_tag="ACGEUI_000260"
     CDS             complement(45877..46392)
                     /gene="lacB"
                     /locus_tag="ACGEUI_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFF8165128.1"
                     /translation="MKIAIGCDHIVTNEKMAVSDFLKSKGYDVIDCGTYDHTRTHYPI
                     FGKKVGEAVVNGQADLGVCICGTGVGINNAVNKVPGIRSALVRDMTTALYAKEELNAN
                     VIGFGGKITGELLMCDIIDAFIKAEYKETEENKKLIAKIAHLESHHANQEDPDFFTEF
                     LEKWDRGEYHD"
     gene            complement(46407..46832)
                     /gene="lacA"
                     /locus_tag="ACGEUI_000261"
     CDS             complement(46407..46832)
                     /gene="lacA"
                     /locus_tag="ACGEUI_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFF8165129.1"
                     /translation="MAIILGADAHGNALKELIKSFLQEEGYDIIDVTDINSDFIDNTL
                     AVAKAVNEAEGRLGIMVDAYGAGPFMVATKLKGMVAAEVSDERSAYMTRGHNNARMIT
                     IGAEIVGPELAKNIVKGFVTGPYDGGRHQIRVDMLNKMA"
     gene            complement(47072..48523)
                     /locus_tag="ACGEUI_000262"
     CDS             complement(47072..48523)
                     /locus_tag="ACGEUI_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFF8165130.1"
                     /translation="MMQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKAL
                     EGGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGISLNIIDVGWAPLATITWGSPY
                     TLYFLLVMLVVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYAARNHLPVFVSLLI
                     ATVAVILVGVLKIINSDLMKPTFDDLLGTGPQSPMTSTHMNYMMNPIIMVLDKIFDKV
                     FPGLDKYDFDAAKLNKKIGFWGSKFFIGMALGFVIGIMGDPHFTVESIKNWFGLGFTA
                     GACLELFSLIGSWFIAAVEPLSQGITNFANARMHGRRFNIGLDWPFIAGRAEIWACAN
                     ILAPIMLIEAVLLSKVGNGILPLAGIIAMGMTPALLVVTRGRLIRMIIFGSLLLPLFL
                     LSGTMIAPFATELAKKVGAFPAGTSAGSLITHSTLEGPMEKIFGYVIGQATTGQIASI
                     ITLIIFVAIYLGLFAWYANQMKARNAEYAKTMK"
     gene            complement(48549..48854)
                     /locus_tag="ACGEUI_000263"
     CDS             complement(48549..48854)
                     /locus_tag="ACGEUI_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8165131.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIETQMSDRGYDVHCDAVMVKDIT
                     EELVSRYDIFTPIAKTDLGFEIPIPIVEAGPILYRIPIMSEPVFAELERVIKENHLQ"
     gene            complement(48847..49320)
                     /locus_tag="ACGEUI_000264"
     CDS             complement(48847..49320)
                     /locus_tag="ACGEUI_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8165132.1"
                     /translation="MFPNILFTEARTQPELFDLVASHLEKVGYVTQEYHQALVEREAV
                     FPTGLKVDLKDGSDILYAAIPHTETKYCLVDQVVYVRNSQALTFKHMINPEEDCLVTD
                     FFFIINSQNEGQTTILSNLITFFITKGNLSYLASLKDDKQAISNYLIEKGVFQHD"
     gene            49557..50327
                     /locus_tag="ACGEUI_000265"
     CDS             49557..50327
                     /locus_tag="ACGEUI_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8165133.1"
                     /translation="MLKRERLLKITEIVNEQGIVTVNDIIQTLNVSDMTVRRDLDELE
                     KAGKLIRIHGGAQSITMPNKKERSNIEKQTVQTKEKWELASYATQLVNDGETIFIGPG
                     TTLECFAEQLKNRQIRIVTNSLPVFNILQDSETIDLILIGGEYRSITGAFVGSLASQN
                     ISSLKFAKAFISCNGIYKNDIATYSETEGEIQKLAFNNSIEKYLLVDNQKFNAYDFFI
                     FYHLNNIDAVVTDSQITEDVIERYSQFTQLLVAHENKE"
     gene            complement(50531..50734)
                     /locus_tag="ACGEUI_000266"
     CDS             complement(50531..50734)
                     /locus_tag="ACGEUI_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HFF8165134.1"
                     /translation="MEKHYQVTGMTCDGCARTVTEKLSAVPGVQSVQVNLEKGEAKVT
                     GRPLTFLIKRALKDTKFELYKWS"
     gene            complement(50748..52979)
                     /locus_tag="ACGEUI_000267"
     CDS             complement(50748..52979)
                     /locus_tag="ACGEUI_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8165135.1"
                     /translation="MAKEIFLVEGMSCASCALTIEKTVNQLPEVEQAVVNLATEKLTV
                     TYQDDDLETAKVIQAVKAAGYGAQVFDEHAKHMQTGRRDEDLKVIWSQVLWSALFTIP
                     ILYLAMGHMVGLWLPPFLQPDRYPFVYSFSQLLLTIPVLVLNRHYYHNGFKALFKGHP
                     NMDSLVALATSFAFAYSLYGVVEISLGRAHFVHSLYFESVVVILTLIGLGKYFESRSK
                     GRTSQAIQKLLSLKATVVRVWRNSQWQLLPLEEVSYDDLVLVQPGEKVSIDGIIVEGH
                     SSLDESFLTGESLPVEKGEKDNVFAGSLNGPGALTIKPDKLGNDTLLAQIIQLVENAQ
                     ENKAPIAAIADRVSGVFVPVVLVLALLTGVFWLVFMQESLRFSLTTAIAVLVIACPCA
                     LGLATPTAIMVGTGRAAENGILFKGGDILEQAHQVDTVVFDKTGTITEGKPSLQKLLT
                     FSGDDHLILQEAASLEAYSQHPLGEAIVRAAQEAGYDSLPVEEFESLTGLGVTGQLEG
                     RRLAIGNAALMGILGIDLSCVQSVCAKASEKGQTLVYYAKEGQLRALFSIADAVKEDS
                     QATVEALHQLGIHTIMLTGDHDATAKAIASQVGITDVISQVLPDQKAGVIADLRSQGR
                     KVAMVGDGINDAPALAVADIGIAMGSGTDIAIESADVILMKPDMLDLVKAMSLSRVTM
                     RIVKENLFWAFIYNVLMIPVAMGLLHLFGGPLLNPMLAGFAMSFSSVSVVLNALRLKG
                     KTI"
     gene            complement(52979..53413)
                     /locus_tag="ACGEUI_000268"
     CDS             complement(52979..53413)
                     /locus_tag="ACGEUI_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFF8165136.1"
                     /translation="MQQISAAEWEVMRVVWASGDIKSSDIITILRKKYQWSDSTIKTL
                     IGRLVKKNFLTSYRQGRAYIYQALLDETLLQKEALATVLDGICQRQHTRLLLERLYHL
                     PMTLEEIGAFQELLEVKKENAVLEVPCNCLPGQCHCQRKETY"
     gene            53597..54571
                     /locus_tag="ACGEUI_000269"
     CDS             53597..54571
                     /locus_tag="ACGEUI_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8165137.1"
                     /translation="MRKTLVTLGLLLTLCLPTPVAASSRSWKSWFIEQYFWLKRDKSY
                     YSKQDDPSFQRYLDACREQSDKPYQLDTNLVNGPLVQENLYGMQVYSWNDNGKPDQKT
                     IIYLAGGSYLNNPTTYHINMLKTLSTSLDAKIVLPIYPKAPRYTYNYTMPKLVNLYQH
                     YYHKNQNVFLMGDSAGGGLALGLAHALHNESVPQPKQLVLLSPWLDVTMSHPEIPEYE
                     DADPILSSWGLKRVGELWAYSADNTNHIYVSPKNGPITYLPPITLFTGTREIFYPDIR
                     DYAAKLKAANHNITFITQEGMNHVYPIYPIEEAKTAQYQIIDAINKTP"
     gene            complement(54705..55055)
                     /gene="rbfA"
                     /locus_tag="ACGEUI_000270"
     CDS             complement(54705..55055)
                     /gene="rbfA"
                     /locus_tag="ACGEUI_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFF8165138.1"
                     /translation="MANHRIDRVGMEIKREVNDILQKKVRDPRVQGVTITEVQMQGDL
                     SLAKVYYTIMSDLASDNQKAQTGLEKATGTIKRELGKQLTMYKIPDLVFEKDNSIAYG
                     NKIDQLLRDLDNKS"
     gene            complement(55260..58121)
                     /gene="infB"
                     /locus_tag="ACGEUI_000271"
     CDS             complement(55260..58121)
                     /gene="infB"
                     /locus_tag="ACGEUI_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFF8165139.1"
                     /translation="MSKKRLHEIAKEIGKSSKEVVEHAKYLGLDVKSHASSVEEADAK
                     KIISSFSKASKPDVTASQTVKPKEVAQPSVTVVKETGSEHVEKTQVSKPKSRNFKAER
                     EARAKEQAARKQANGSSHRSQERRGGYRQPNNHQTNEQGDKRITHRSQGDTNDKRIER
                     KASNVSPRHDNHQLVGDRNRSFAKENHKNGRFTNQKKQGRQEPQSKSPKIDFKARAAA
                     LKAEQNAEYSRQSETRFRAQQEAKRLAELARQEAKEAALKAQAEEMSHREAALKSIEE
                     AETKLKSSNISAKSTADNRRKKQARPEKNRELTHHSQEGQKKNKKSWNSQNQVRNQKN
                     SNWNKNKKTKKGKNVKNTNTAPKPVTERKFHELPKEFEYTEGMTVAEIAKRIKREPAE
                     IVKKLFMMGVMATQNQSLDGDTIELLMVDYGIEAKAKVEVDDADIERFFEDENYLNPE
                     NIVERAPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIEEAGKKITFL
                     DTPGHAAFTSMRARGASVTDITILIVAADDGVMPQTIEAINHSKAAGVPIIVAINKID
                     KPGANPERVIAELAEYGIISTAWGGECEFVEISAKFNKNIDELLETVLLVAEVEELKA
                     DPTVRAIGTVIEARLDKGKGAIATLLVQQGTLHVQDPIVVGNTFGRVRAMVNDLGRRV
                     KSAEPSTPVSITGLNETPMAGDHFAVYADEKAARAAGEERSKRALLKQRQNTQRVSLD
                     NLFDTLKAGEIKTVNVIIKADVQGSVEALAASLVKIEVEGVRVNVVHSAVGAINESDV
                     TLAEASNAVIIGFNVRPTPQARQQADTDDVEIRLHSIIYKVIEEVEEAMKGKLDPVYQ
                     EKILGEAIIRETFKVSKVGTIGGFMVINGKVTRDSSVRVIRDSVVIFDGKLASLKHYK
                     DDVKEVGNAQEGGLMIENFNDLKVDDTIEAYIMEEIVRK"
     gene            complement(58141..58443)
                     /locus_tag="ACGEUI_000272"
     CDS             complement(58141..58443)
                     /locus_tag="ACGEUI_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFF8165140.1"
                     /translation="MTNLERLSSLIGLAQRAGKVISGEELVVKAIQHQQVILVFLAND
                     AGPNVTKKVTDKSNYYNVEVSTVLNALELSAALGKPRKVAAIADAGFSKKMRTLME"
     gene            complement(58436..58732)
                     /locus_tag="ACGEUI_000273"
     CDS             complement(58436..58732)
                     /locus_tag="ACGEUI_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFF8165141.1"
                     /translation="MSKVKKIPLRKSLVSGEIIAKRDLLRIVKTKDGQVFIDPTGKQN
                     GRGAYIKLDNQEALMAKKKQVFNRSFSMDIPESFYDDLIAYVDHKIKRRELGLD"
     gene            complement(58748..59905)
                     /gene="nusA"
                     /locus_tag="ACGEUI_000274"
     CDS             complement(58748..59905)
                     /gene="nusA"
                     /locus_tag="ACGEUI_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFF8165142.1"
                     /translation="MSKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSES
                     CVIEFNEKTADFQVFTVREVVEEVFDSRLEISLKDALAISSAYELGDKIRFEESVNEF
                     GRVAAQSAKQTIMEKMRRQMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQ
                     LSHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDG
                     TVEIMSVSREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGL
                     EIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDEDLKRATVVVPDSKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEYDRLEAEKEAATAVEEPVVDDVMTAEEV"
     gene            complement(60080..60616)
                     /gene="rimP"
                     /locus_tag="ACGEUI_000275"
     CDS             complement(60080..60616)
                     /gene="rimP"
                     /locus_tag="ACGEUI_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFF8165143.1"
                     /translation="MDSQGPIILEKSIKIEEVIKIANTSIIDIVTKTVTPEIKAPYEL
                     VDVEYDKMGSDYILSILVDKEGGITVEDTSDLTNIISPLLDTIDPDPFPNQYMLEVSS
                     PGLERPLKTADSLKAAVGSYINVSLYQAIDKVKVFQGDLLAFDGETLTIDYLDKTRHK
                     IVNIPYQAVAKVRMAVKL"
     gene            complement(60861..61040)
                     /locus_tag="ACGEUI_000276"
     CDS             complement(60861..61040)
                     /locus_tag="ACGEUI_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HFF8165144.1"
                     /translation="MLTYDEFKQAIDHGYITGDTVAIVRKNGQIFDYVLPHEKVKNGE
                     VVTDEKVEEVLMELE"
BASE COUNT        18260 a        13361 c        10934 g        18556 t
ORIGIN      
        1 aaaacagaat tctttaatcg tattaaagaa aaggtacaac gttaagggaa ttagcaactg
       61 atttttcaaa cgttaaccta aaacaaaata gacttattta aaattgttat aaatagcaac
      121 taatgccata agagagtata catgaaaaaa acacgtttcc atataccgaa gacgtgtttt
      181 ttcatatgtc attttcttag cataataatt tttttcatgt tcactttctt atgcggagtc
      241 caatccgttt gctgttcgtt ttgtccagct tttagatttc atacagtcgc aaagaaagga
      301 attagtattg tgctaaagca cccattggat cccaaggagc tagtacttgt ggctcttttt
      361 cataagccgc taactcgtca gctgttaaga attctttacg gacaacaatt tgataggtat
      421 attcatccat ccaagcatct gaagcaacaa agtaaccttt gtcaccaacc ttctcacccc
      481 atgaattttc aaccttccat tttaacggtt ttcctgtttc atctaagtca acacctgtta
      541 aaaccatagc atgtgtcatc aaactttcac tataatccaa acgtccagcc ttatcttgac
      601 taagattgat atccatacta gcttcaaaat cgtaagtatt agttgctaaa atgccttttt
      661 gacgatctga aacttgaccc acatcagagc caaaccaaac ggactcacca gcctgcatct
      721 gcttgatagc tagttctttg aaacgtttca tatcaaggtt gaggtagcga acctcacgac
      781 taccgaccac atttcctagc atctcaacag tgtatgattt gccataaggc ttatcagcag
      841 ttggcgcatt aataacagac acataatcag aaagctttaa gccaacaaat ttgtcgtaaa
      901 acgcttgcgg tgtcaaacct ttctcaacat gatagtggtc atctttatca cggtaggcaa
      961 aatcaaaatg acgaggtggt aaaccaaggg tcatggccaa gaagttaaaa atttcctgaa
     1021 gaagctctgc ttttcgatcc tcaacttggt ctgccttagc acctgacgca atcaggtcac
     1081 gaagaatttg agcatcttgg cgcaataatt tattcaaata ctggtttaat tcacgactgt
     1141 tacttgatga gattgattct gggtaaacag attttggcac cactccatac ttctcaaaca
     1201 aggaaaccac catgtcccac tgaccaccat cttgttgtgg aacatccaag agaaacttaa
     1261 ctttacgact ggttaattct tggtcagctg tagcaatgac ctgctccata aaccagttag
     1321 ctttttcata cttatcccaa aagaaagtat gagcttgtga cagttcaaaa ttttctaact
     1381 taaattctgt gattaatttg tgtcggaaag tgttcaaggc agcaaacatc cagcaacgac
     1441 cagaagcctt ttggttagat accttatctt tagttaaatc aatagaaaac acaaaatcat
     1501 tgtccacttc actttgacgg gtttctaatg attttagcaa accattatgc gtaacggcat
     1561 tctcaatagc tgaaaatttt gcattggctt cgtaatgagc aaataattgt tctgtaaatg
     1621 tctctgttaa tgctgacatt attgtcctcc taaaatatta agataagctt cacctgaaat
     1681 aatattggca agatggttat tatccagcca ttctattcta aaggattact tctattatag
     1741 accttatttg attttcttca agaaaaaagt taggataacc taactttttt tgttttcttt
     1801 ttttactctt tgaacatcat ctcttatttc caaaaatcat caaaaatggt gattggcagg
     1861 tgacgcttgt gttgtccttt atgccaccat ttttcaatag ttgcttgagc tactttggaa
     1921 atcagcttcc cttccaaata gtcgtcgatg tcttgatagg tcacccctag agcaacctca
     1981 tcagccaagc ctggtttttg atcttctaaa tcagcagttg gaactttttc atacaaagca
     2041 gcatctgctc ctaaaacttt caaaagagcc tttccttgac gtttgttaag cctaaagagg
     2101 ggaagaatat ctgctccacc atcaccaaat ttagtaaaga aaccagtgat gttttctgcc
     2161 gcatgatccg ttccaataac agcaccagcc atttggccag caatggcata ttgactgatc
     2221 attcgctgac gtgctttaat attaccttta ttgaaatcag aaatctcaac gcctgctgct
     2281 tgtagagcct ctacttgacc gtccacagct gccttgatat taatggtaag ggtctgatct
     2341 ggtgcaataa aagccaaggc tttttgcgca tctgcttcat cagcctgaac cccataaggc
     2401 aagcgaacag ctataaattg gtaagcttga tcacttgctt cctcccgtaa ctctgcaatg
     2461 gccatttggg ctagctttcc tgcgagggta gaatcctgac ctcccgaaat gcctaagaca
     2521 taagttttta aaaatgagtg cttacgcaaa taagctttta aaaaatcaac ggctttccga
     2581 atttcttctt gtgggtcaat gctagccttg acgcctaatt gtcgaattat ctcttcttgt
     2641 agtgtcatgt cattcactct ttccataagc atctttgcga atgttatcaa ttaaggccat
     2701 cttgttttgc cacacatcac gggctaagtc tactgggtag tcttgaggat ttaagacacg
     2761 cttgtattca tcccaaagct tgtcaaattc ttttttagca taagccttga tttcagctaa
     2821 ggtcggtagc tggtaaacta attctccctt gacaaaaata tcaactaaga gaggaattgc
     2881 atcaaattct ttaacggttt tcttaatata ggtataggtc ggatggaaca tctcaatctc
     2941 agttaattca ttaacattaa tatcagtaaa cgtgatatag tcgccttctg atttgccttt
     3001 ttcacgactg gtgatgcgcc aaacttgttt tttacctggt gtggatactt tttctgcatt
     3061 atttgataat ttgatcgtat cacgcatgct gccatcttct tgctcaatag agacaatctt
     3121 ataaacagca cctaaggcag gctgatcata ggctgtgatc agtttggtcc caacacccca
     3181 aacatcaatt ttggcctttt gcatttttaa gttgaggatg gtattttcat cgaggtcatt
     3241 agaagcatag atcttagcct cggtaaaacc agcgtcgtct aactgctgac ggactgtttt
     3301 tgagaggtaa gccaagtcac cagagtccaa tcgaacacct agaaaattaa tcttgtcacc
     3361 catttctttg gctactcgga tagctgttgg cacacctact ttaagtgtat cataggtgtc
     3421 aacaagaaat acacaatctt tatgggtctt agcataagcc atgaaagcat cataatcatt
     3481 accataggct tgcactaaag catgagcatg cgtacctgaa acagggatat caaagcgttt
     3541 acctgctctc acattacttg ttgcatctgc tccaccaatc atcgctgcgc gggttcccca
     3601 aatcgcagca tctagttcct gtgcacgacg ggtgccaaat tccaaaaggg gttcatcctc
     3661 aatcacagaa cgaatacgag cagcctttgt agcaatcaag gtctggaagt taacaatgtt
     3721 taaaagagct gtttcgacca actgacactg acctagaggg ccttctactt gcacaatcgg
     3781 ctcattagca aaaaccaaat ctccttcttt agctgaacgg atggtcaatt ctaatcgtaa
     3841 ctctttaagg taggtcaaaa agttttctgg ataacccagt tcttcaaggt aagccaaatc
     3901 tgtttctgaa aattgaaatt gctctaggta ctccaccatg cgctgtaaac cggcaaaaac
     3961 agcataacca ttgttaaaag gttccttgcg gaaatagact tcaaacaccg catgacgatt
     4021 atgaatgccc tgctcaaaat aaacttgcat catattaatc tgatacaaat cagtatggag
     4081 agttaaacta tcatctttat acataaaggg gactcctttg aattattgtt actattataa
     4141 catattttaa aaaagactct ttggctaggg tttagaaata acttgagtta tcgctaaagt
     4201 aaaaaaagaa ctgatccatc atgacatcct caaataagaa agagttaatg gattcagttc
     4261 tatatcgcta attattattt aaatttgaca ttgctataaa tcccaaaaac aataatccaa
     4321 atcgttgctc caatggcgcc gatataggtg gattcttgta agaaaagaga gataaagaca
     4381 aaggcaaaga aagcaagggt caaaggcgtt gtcactttat attttggcat caaataacca
     4441 tctgccataa agtccttaga ttgacggtac ttccagtgag caatcatggt taaggcgtag
     4501 atagcaatat atacacctga tgaggaggcc gtaatgagtg agaacgcatc tgcaactcct
     4561 ggtaggatat taatcaaagc tgagatacca accacaaccg ctgaagcaat aatggcacga
     4621 cttggcaccc cttgtcgaga taaagtatta atctttaagc gattggtcaa agcgtttgga
     4681 gtctcattag caatctgata gagatgacga ccggttgaat aaagggttga gttaagcgct
     4741 gacgctgctg atgtcaagac cacaaagtta atcaaggctg ctgcccattt aataccaatc
     4801 aatttgaaca ccatcacaaa aggagattca tcaactggta actgatgcca tggcacaatt
     4861 gccataatag agaccaaggc tcccacataa aagatcacaa tacgggttgg aatttcttga
     4921 atagcctttg gtagaacttt tcttgggtta gccgtctcag aagtggtaat tcccacaaat
     4981 tcgatagctt gataagcaaa gaaaaccatt tggaaggcca taaagaactt tagtttccca
     5041 tttgggaaca tggaaaaatg gtcaaaaata ttggaaaggc tagcatggcc tgtgtgagtt
     5101 tcaaaacctg tcaataccat gaaaatagct gttgcaatca aagctaaaat agctaaaatc
     5161 ttaatcatgg caaaccaaaa ctctgtttcc ccaaaaacgc gcacagcaat taagttgatc
     5221 gaactaagta agactaagaa aaccaactga atcaaccagg ctggccagct tgggaaccaa
     5281 aattgcacat aagcacctac tgctgtgatt tctgccattc caataaaaat aagggaaatc
     5341 caataggata gacccgaaaa ataaccccag cctggaccaa tgtatttgga aataaaattg
     5401 ataaaggtgt gttgatctgg atcataatag agcatttcac cgatggcacg catcatcata
     5461 aacatgaagg cacctgtaat catatacaca aaaataatag atggtcctgt tagggcaata
     5521 gagcgtcctg cccctaaaaa gagacctgtt ccaatcgttc ctgcgatcgc aattaactgc
     5581 acatgacgat tttctaagcc ccttaccatt ccattttcta gttcgttatt gtctgtttgt
     5641 tcttttatag acattctaaa ctccattcac taaaatacta tttattatat ccttttttca
     5701 gaaaattttc aacatttatc taggaaagat attaaggaaa actgttcttt atatttctta
     5761 aaggtcttat gcaatttagc ttttttaagg gtgagacaag gaaaaatgaa ggagttttaa
     5821 ccttatgaag agaaactttc aaggtagtga taaacacctt gccctgcaat tgcaccatct
     5881 ccaacagctg tcgtgatttg gcgaaggtct ttttgacgaa catctccaat cgcaaaaata
     5941 ccaggaatac ttgtcctcat atgatcatct gtgataatcc aaccttctga atcggtaatt
     6001 tctagatcct ttaccatacc tgtaacagga ttcataccaa catagataaa cacacctcca
     6061 aaagcgtggt cagtgacttg gcctgttttc acattttcga ttaggacatt ggagacctta
     6121 atgtcgttac cttgaatttc tttgacaact gaatcccaga taaagtcaac tttgtcatta
     6181 gcgaaggcac ggtcctgcaa gattttctga gctcttagtt gatcacggcg atgaacaacg
     6241 gttacctttt tagcaaactg agtaagataa atagcttctt caactgctga gtcaccacca
     6301 ccaacgacca aaagatcctg gtcacggaaa aacgcaccat cacatactgc acaataagaa
     6361 actcctcgac tagtatagta ttcttcacct ggaacaccta gtacacggta tttagctccc
     6421 gttgcaataa taaccgtttt agcttcataa ctagcatctt cagtcaacac acacttatag
     6481 tcaccaaaat tttctatttt ttgaacaata ccataaatgt tttcaacgtt aaacttctct
     6541 aaaggttcgt acattttcat agctaattct ggtccagata tatgatcata tcccgggtaa
     6601 ttctctatat caaaagtgtt gttcatctga ccaccaggtg ccccttgttc aataatggca
     6661 acagataaat tacttctagc agcgtataaa gcggcagtca ttcctgctgg tcctgaacca
     6721 ataattaatg tatcgtacat gatttttctc ctttaatcta ctcttgccaa tattttagca
     6781 atgataatcc cttcttgcaa tgattaggat tttatgacaa ccaaaatact cataaaagca
     6841 aacgataaaa ttgggatggt taaaatagca accattagca tctcataaag aaaagcttgt
     6901 ctttcaatca atgttttatc tcttttggtt tttgctcgcc ataaagtcca tattaaacaa
     6961 attaaaatca tagtaaggct tgagagaatg agacctttta cataaaggct aaagatttct
     7021 actaacatat ttccacctag tcattatttc tttcccataa gtttttctaa aaaacataac
     7081 aagaaaagcc cagggatacc tagacctttt tattataaca aaagaataac attaaatgta
     7141 tgtttttgaa tagcttgcaa aaaattcttt ggttcgttct ttttgaggat gtctaaagac
     7201 ttcttcaggt gttccttgtt ccaagatttt cccttgatct aaaaagagta ctctatctgc
     7261 cacttggtaa acaaaattca tgtcgtgact cactaaaacc atggtctgtc cggatttagc
     7321 agcatcagta atagactttt ccacttcgcc aactaattcc gggtcaagag ccgaggtcgg
     7381 ctcatctagt aataaaacat ctggtttcat agccagagct cttgctaaag ctacccgttg
     7441 tttttgtccc cctgataaat gacgtgggta atgatgtttt cgatctgcta aaccaacctt
     7501 agccaattct gcttgcgcca gtttagtcgc ttcttggtca gacagttttt tgaccacctt
     7561 aagaccttct ttgacattct caagggctgt acgacgctca aaaagattaa attgttgaaa
     7621 aaccatggca agtttccgac gtaaaataag gacttgctct gtggtaatgg tctcaaaatc
     7681 aaccgtaaag tcaccaatgc taatggagcc tgaatctggt ttttccaaat agtttaggct
     7741 acgaaggaag gtcgatttcc ctgctcctga agcaccaact aaagcaatca cttgaccttt
     7801 ttcaatatca agagccaaac tatctaaaac tttttgccct gagaatgtct tgctcaaatt
     7861 tctaattgta atcatcaacg caattctcct aacttttcat ttctagcttg ttctggtgct
     7921 ttgatggcca tcttgttttc aatcaagcga cctacttgct ccatcaaaat actgatagac
     7981 caatagacaa gagcaacaga gatataacgt tcaaaataac ggtaatctga tccgcctaga
     8041 atttgagcct gggcaaacat ttcgacaatc cctgcattaa aggcaagcga tgttccttta
     8101 gttaagccaa ttaacccatt aatcaaggtt ggaatagcca caacagttgc attaggaata
     8161 atgacacgac ggtaaacctg cacagaagtc atgcctagac tttttgctgc ttcaatttca
     8221 cctgtatcta ctgataaaat ggctgcccga atagtttcac tagcgtaggc tgcttcatta
     8281 aaagcaaagg ctgtaatggc aaaaatagaa gccggaattg cattaacatt ccaatcaaag
     8341 ccatacttct gattgagaaa ttttagaaag aggggaatac cgtagtaggt caacatcaac
     8401 tgtaccaaaa taggagttcc tcgcaagaaa ctcacaaaaa tagcttgaat agggtatagt
     8461 agcttcactt tattaatttt gacaagggca aaaatcaaag ccaaaaccaa gccaaaggtt
     8521 gctcctgata gtgtcaaacc aagcgtaatt ggaagccgtt ggataatatt tggaatggca
     8581 tcaaaaacag catgccaact aaaaagtttc ccatgtggaa tcggtgagat gaggtaatca
     8641 taaaaagccc aaccactggt taaaaacact gatgtcatgt atttcccctt tgtatttgtt
     8701 tgtcttaata ttctaacact cttagaccca catgtaaaat agtaaattcg tattatagca
     8761 ataagcaaaa gctatgatca ctttttgtgt taacaaatgg taaacaatac aagtagcagg
     8821 tgtaaaaaaa gcaagattag atgccaattg atcacttgct tgctctttgt tcttaaaaat
     8881 gttgtttttt ggccttacgc tcagcgcggc ccttagcacg attttcagca cgacgttctt
     8941 tgcgacgttt ttcatcaacc gcccactgaa tttttttctt gtaacctggt ttgacttttt
     9001 tctttttctt tttgaccaaa ccaatcattt ctgtgtctaa tttttgatag gctttttcac
     9061 ggttttgacg gcgatcacgg tcataggtat cttgaaattc tccatttttg agcaccttcg
     9121 gtgtaaaggc aatccccatt ttttccagct ctttgatatc tgagtcgtca cttggctggt
     9181 aaagtgtaat ggcagttcct gccataccat ttcgacctgt tcggcctaca cgatggacaa
     9241 agaaagacaa gtcttgtggg atggcgtcat tgatcacatg gctgacccct tcaatgtcaa
     9301 tcccacgcgc tgctaagtct gtggcaacaa tgtattcaaa gtccagtttt ttgacttggt
     9361 tcatgatgcg cttacgttct cttggaggaa tgcccccatg aatcttagcc acttttaagc
     9421 cattagctgt taaaaaggca tgcaaatcat ctgcgcgttc cttggtattg acaaaaagca
     9481 tagccatata aggctgcatg gttttgagaa tctctaacag ttgaccattc ttatcacgcc
     9541 cttttgtcga cacaagccag ttatcaatgg tatctgcgat aacggtcttt gtcttgattt
     9601 gttcaataac aggattggtc aagtattttt tcaaaaaggg ttgtaatttc tgtggaatag
     9661 ttgctgagaa caccaaaatc tgaacagatt ttggaagcga agctgcaatc ttatctaccg
     9721 tatccaaaaa gcccatgtcc atggtcatat cggcctcatc cacaacaaag gtcgttgctt
     9781 tatgaatcgc taaatcacca gacttgacca agtcataaat acgacctggg gtcccgatga
     9841 caatatgcgg ctggctgtct tttagttttt caatctgacg gagtttgtct gtgcctccaa
     9901 catagttggc caaacgaatc tcttcttgga agtgcttagc aatctgttta caagcatcaa
     9961 aaatttgagt tgctaattca cggctaggtg ctgtaataac gacttgcact tctgctttag
    10021 cttcatcaag tttttcaaaa atgggcaaca aaaaggtgtg ggttttccct gaacctgttt
    10081 ttgattcccc aactaagtca cgaccggagt taacaatagg aatcagccgc ttctggacct
    10141 ctgttggatt gacaaagcca atttcttcca gcgcttgctg aacgtattgc ttaaaatgat
    10201 aatctttaaa tgacatgttt tacctcgttt tcttccatat tatatcatta ttgttagaga
    10261 caagcaaaag gagtgggaca acactcgcaa aatcagcatt tgtgggtcat ctccactctc
    10321 ttattgatga tgttaatcaa aatgctattg gtgactattc ttgataatcc cccttagaac
    10381 acgtataaga tagctaaaac tagtaaacta gctagacttc ctactcccca caaaaaagca
    10441 tctacctgcc attctgacca cttcttgcct ttaccagaca agcctcccaa ttccaaatgg
    10501 tggtggaaag gcgtcattct aaaaatacga cgaccttctc catatttttt cttggtgtac
    10561 ttgaaatagg acacttgcaa catcacagaa cttgtttcaa gaacataaac gatcccaata
    10621 atcaggagag tccattcttg acgaagcgca atagaaatgg cagccaacat agctccaagg
    10681 gctaaactac ctacatctcc cataaatact ttagcaggtt tgtgattaaa gcagaagaag
    10741 ccaagcaagg ctccaatcat tgctccaatc agtaacaaaa catcaaattg actctgaacg
    10801 taggcaataa cgccgtaagt cactagacta atcaccactg agatagaagc taagccatcg
    10861 ataccatctg tcaaattaac ggcatttgaa aaaccaacca cccaaaacaa aacaaagaat
    10921 aggtagaaaa ttcccagcgg cagctgataa ccaaacacat taatagagga aatgccactt
    10981 ggactaacat gtaaaaaata gaacatcaat cctccaacca actgcaaggc taacttttgt
    11041 ttggctgtca atccttcatt gatttgctta aaaattttta aaaaatcatc caaaaatccg
    11101 ataatcccgt aaatgacaac aattgataaa atgcctgaaa tcaaagctaa actttgagtg
    11161 tttttgatcg aaaatagact aaccaataac gatactgccg tagctactaa aagaaaaaca
    11221 gttcccccca tagtcggtgt tccagcttta gctaaatgtt gcttgacatc ttcatgcatt
    11281 tgttgcccac caattttttt caattgatag aatttgataa aataaggcat tgtgaaagca
    11341 gataccataa aggatatgat tgctgcaata agggttaaaa acatgttaat ctcctaaagt
    11401 aatggtcatt ttttttattt ttttcaagga cttaccaaca tcaacacttt gtttcataac
    11461 acgaccagaa tctgttcctt taaagctgat gtctattcca gtccatttag caaaggtttt
    11521 aacattggat tttgtccagc catacatgtc tggtacctcc acaaaacggt ctgataatat
    11581 gagaacttgt tggttttctg ttaacgtttg accgggctga tgcgatactt ttttgatctt
    11641 gctgccagta ccaaggacaa ctggctggac aaggtttcga cgcaactcgc ttgatgtctc
    11701 accaggattc tttcctacaa agtttggtaa tttataagtt gtttgacgat tagcatctga
    11761 tactactggc ttagttagtg tatcttgcat taagtatgct tcttccaata ctgggttaac
    11821 cacatcttgc caaaaaaggg gaccaaaatg ttgtggttta gtcatagtaa catacatcaa
    11881 aaagtctggt ttatcagctg gcaccattgc cacaaccgaa tagacatagt tagtcaatcc
    11941 accatcttga taaccacttc catcttctga accaatttgt gctgttcctg atttaacagc
    12001 aacaggtaaa tcacccactt taataattgg tccaaatgtt tttgaataga gtgttccaaa
    12061 ctcagggtct gttcctacac caatcatgta ttgtcttgtt tcactagcgg ctttttttga
    12121 tacaggtttt ccaacaattt ctttatttgc cgttctaaaa cttgctgtgt taggatcata
    12181 aatttgactg ataaattgtg gctctaacat ctcaccatta ttagaaatag cagtaaaggc
    12241 tctaagcatt tgaatctggg ttacagaaat cccttgacca aaagcgctca tagcttgagt
    12301 cacgatatta tcagaaggaa atatacctgc gtcttcatct tttaaaccaa aacgagtagg
    12361 aaaaccaaag cggaacttcg tcaaataatt catccatttt gcattaccca ttttttgttc
    12421 aagtttagtc atcccaacgt tacttgagaa ggcaaaccct tgtgcataat tcatgtactg
    12481 tcctgtggaa atgccttcgt taatagacca gtcttgaata gtagcatctg caattgtcaa
    12541 accattagca ttgctaaagg tttcgtttgg gttgaaaact ttatcatcaa tagccgctgc
    12601 cagagtcatc actttcatgg ttgaacctgg ttcaaaattt ccttgatgaa gtgcactgta
    12661 ccatttgtag ttcgtatttt caagtccttt cagagtatca gcattataag tggggcgttg
    12721 tgttgttgcc aaaatttcac cagttttagc attaacaagt gttgcactgg ccaactgacc
    12781 atttgatttg gcttgaaaaa catccatctg ggtttctaag aaggtctgga taggctcaga
    12841 tagcgttgtg taaatatctt tgccatcaat agctttcttg acagtcttac ctgtacccaa
    12901 gagtgtggtc ccatttcgat ctttttgata agtaataaca ccatcttgac ctgataaaat
    12961 tttatcaaaa gaagcttcta gacctgtttt tccaactaaa ctcttaacac ctgtcttttt
    13021 atcttctgtt agagatgcta ggcctataaa ttctgaagca aatgtcccat ttggatacat
    13081 acgaccagga ctagttgtga aagcaattcc cttgattttg gcatcttcca tagccttttg
    13141 aatagtagac atagtactat atgaaatgcc agaccctgac ggtccaaacg agacttggaa
    13201 aagtccttta cgcttaagct gtttaattac atctgttttt ttcattccca gatgcttttt
    13261 taaaatatca gctactgttt catactgaga aggttgtaca tataactttt catctgaagc
    13321 cgagacaaat gatttatcca aaattgcgta tatgctataa gtcgtagaat ccactgcaat
    13381 agctgtacca tttcgatcat aaatggtccc acgcttagct tggatcgtaa cggtttcttg
    13441 ataaaccttc ttcgcccctt ctgacaaact aactccaaac ttttgatctg ttccaataat
    13501 aatcatgaaa ttaataatga aaataaagaa aataaagata gttaagagca tcatattttg
    13561 tccaactcga acgcgatttt cgactggagt tctcctatcg cgcacaacat aatctaaaac
    13621 atatttttgc cattttttca tagattactc gactttcttg atattattat tacggttgct
    13681 taagcctgct ttaccagcaa tatctataat acgatcacgg cgagataact catttacttc
    13741 ttgcttggca ttattaagct ctagtttttg atccgaaata tgactattta aactagtgat
    13801 ttcttgttgt agctgcaact tacgactttg caggtaaata atacttactg ccatcgtaat
    13861 cgctgttaca atgatagcag tataaaaagc cttctcaatt ctagagaaag tttttattcg
    13921 tttttgcaag gcatttgtaa ctgcctgagt tcttttttca tttgtcatag gcttatttcc
    13981 gaattttttt ggcaacacgt agcttggctg agtgtgccct tttattagct gttaactctg
    14041 aatgactagg taagatcggc ttacgtgaaa caagttcaaa cttaggtttc atatcttcag
    14101 gaattagagg aagccctttt ggcacatcca ccgtactagc ttctttaaac aactgcttgg
    14161 ttaggcgatc ttccagagaa tggaaggtaa taactgagat acgaccatca agggctaata
    14221 attccatagc gtcctgaata gattcatcgg ccgctcccaa ttcatcattg acttcaatgc
    14281 gaatagcttg aaaaatctgt ttagcagggt ggcctttttt cttcaactct ttagctggct
    14341 ttgctgcctt aatcaattct gccaactctg ttgttgtctc aataggctta atagctcttg
    14401 cttgttcaat ttttcgagcg atctgcttgg agaatttatc ttcaccatat ttgaaaaaaa
    14461 tcttaaccaa atcattgaat ggataggtat tcaccacttc gtaagctgtt aagagcgact
    14521 gacgatccat gcgcatatcc aatggagcat cttgtttata agaaaaccct cgttctcttt
    14581 catccaattg cgggctggaa acaccaaggt catataagat accatcaatt tcatcaactc
    14641 caagcgctgt taaacgtgct ttgaggtgtc taaaattatc tttaataaaa gttacctgtc
    14701 ctttgtcaat ataagatttg agggtaactt gtgcattgtc aatagccttt tggtcttggt
    14761 caaaacaata gaggtgccct tcttcaccaa gtttggacaa caaataagct gagtggcctg
    14821 agccacctag cgtcgcatca acataaatcc catcaggctt tatgtcaagc atgtccactg
    14881 tttcgtgaag gagtacggtc acgtgatgaa attcttttgt cataacttta attgtaccat
    14941 acttcaaaag gtttagaaat gaaaaagtaa gagaaaagga tttcattctc gtgggaatct
    15001 tcctcttctc taatcttgtt ctttttgttc gctatcagcc aacttagcaa gcactgactt
    15061 agccactgcc tctagagttt caactgcttg taaaagattt ttagcttcct taatcacaca
    15121 acaatctgtc agcgggttgt ctaaccaaat accaaaggtt ttatcccaaa tcgtatttgg
    15181 taagtcagct aataatagcc tatcgacgtc tgccagctat cttcggcagt tttgaagagt
    15241 aacttgtaag tctattaata attggttgac cttgctcatt atttcgacgt ttgatccagc
    15301 taaccagcat accatctcca aaaaaaaaca aaatagcccc aatttcggac aattttcaat
    15361 tccaaatgaa tcacctgaat ctgatgtaaa agttcttcta aagtaacctt tgcttcttct
    15421 ttttctcttt cattagtcat taagggacct tatagtagct tattctctga tatgtccatc
    15481 cccattaata taaaatttag tgctggtcaa ggcttctaag cccattggcc cacgggcgtg
    15541 cattttttga gtagaaatcc caatttcagc accaagccca aaaacaaaac catcggtaaa
    15601 tcgtgttgaa gcgttgacat aaacagcagc agcatccact aaatcttgaa aggtctctgc
    15661 tgccttaata tcccttgtga tgatagcttc ggaatggtga ctagtatact gatttatcca
    15721 agaaatagct tcttccaaag aggaaacaac cttaacagac atgatataat ctaaaaattc
    15781 ggtctcaaaa tcctctgctt ttgcaggcac tgcctgctca aataacggca aagccttgtc
    15841 atctgcccgc cattctactg gctgaacttg attgatcgct ttttcaagca taggaataaa
    15901 ccttgctgct acagcttcgt gaattaccaa tccctcagct gcattgcaaa cacttgggcg
    15961 ctttgtttta gcgttgataa caatcttagt agctatatct aagtcagctt gagcatccac
    16021 atagatatgc acattcccca cacctgtttc gatgacaggc acttttgctt tttccttaac
    16081 ggtctgaatt aatttagcac caccacgtgg aatcaaaaca tctacataat ccgttgcctg
    16141 catcaattct tcagcgacag catgtgaggg atcctcaact agctggacag catctggggt
    16201 gatccctgat ttttccagac tttgtcggat caacttcacc aaagctttat ttgagtgaag
    16261 ggcatctttt cctcccctta ggatgatcgc attattggtt ttgaacgcca gactaaaagc
    16321 atccactgaa acattcggac gactctcaaa aatcatcgca atcaccccta gaggaactct
    16381 cttttggagg attttaaggc catcgaggtt agtatagccc ttaatgactt gtccgattgg
    16441 atcagctaag tcagcaacct gttgaactcc ctgtgctatc gctttaatcc gttctgaagt
    16501 taagcgcaag cgatccatca tgatgtcaga aataccatgt tctttagcat tcgctaaatc
    16561 tttttggtta gcggctaaga gagtctgtgt atcgtcaact aatgcctttg ctaaggtgct
    16621 taaaaagcga tttttaattt gcgtcgacaa aggtgctatt aaaagactag cttgttttgc
    16681 cctttgccct aaccttctca tatcagtcat gccttacctc ttctatctgc ttgaaccagg
    16741 tgcctagtgg ttgcccttca agcactctta aaatatctct gggattagct ccattcatca
    16801 agaccatctg tcctttgttt tcaaatacca tctgagcaga ctgcacctta cttagcatcc
    16861 cacctgtgcc aaacttactg cccgcaccac ctgctgacgc aatgatttct tgcgtaatat
    16921 tagcaacatg gctgcgtagc tgcgcatcct cataaatagt agggttttta tcaaaaaggc
    16981 catcaatatc agacaacatg attaataaat cagcttttgt aataccagca acaacagcag
    17041 ataaacggtc attatctcca aatttagtcg catgatccat ttcatccaca ctaaccgcat
    17101 cgttttcatt aacaatcggc acaatgccca aactaatcag actttcaaaa gcattggtaa
    17161 cattggctag actttctggg aaaaccacca catctctcgt caataaaatt tgtgacacgt
    17221 tggtttggta gtatgcaaaa atttgtgaat aaagactcat catggctacc tgaccaacac
    17281 tggaaactgc ctgttgttta gcaagattgg tcggacgttt ttccattttc aaaatatcta
    17341 gtccaaatcc catcgctccc gatgatacta aaataacttc cttgccctta ttcatcaaac
    17401 ttgaaatcac aaaagccagt tggtcgattt tttctaaatt aattttacct gttggtagca
    17461 ctaatgaact agtgccaatt ttaattacga tacgtgttac atcttcaaat tgtcgtttca
    17521 tcatacccaa attatagcat aaaaaacagc cccagaggac tgtttttccg aataatgatc
    17581 cctcttcgtt aaagcctctc taagcttttc cagaaggttt tttgaatcca taattgcgca
    17641 agacctaaaa ccattagtat tagacaactc aatagaatac taatcaacag aggttgttgg
    17701 ataatattgg caatgataac agctccaaaa cctagcacac ctgcaactat taaattacca
    17761 aagataagtc ccattgttaa ccattgccca tctcctctgg taaagagttg cgtcatgtct
    17821 tgccatttta aatccagaag ccggtagtca cgacgataca tcaattcccc ttgcacaatc
    17881 agtgataggg cgtagccaag acaaaaagca atcgttagaa gaggatgcag gtgcaacaca
    17941 aagagaccaa acacaagata aatcaccata ggcacaatca gctgcagacc tacaagcaag
    18001 caaaatttat ccatcaagaa tttttttaac gttattggta agcttttaat aaaggtaaag
    18061 ttatcctttt caagtgaaat acctactcca ataaagctgg ttggtgttgc acacatgaca
    18121 cccaaactaa ccccaaataa taaggctacc ccaaagtaat ctggggaaat atgcttaaag
    18181 aaacctgtgc cacgtgacaa acttggaccg ataaaaagca tcacatacat caggggcatg
    18241 agataggttt gtgtcagtaa agttgcattt tgtaacgtta ataggtgatg ttttcgcaac
    18301 aactgcgcca gtgattgatt ctgatgagga cgattaactg gttttttagt ttgcttgact
    18361 ttgttctcgt tactaatata aaaagcctca cgataataag ttggcatcac ttttgtgaca
    18421 ataccatata ccatagctag gataagtaac aatggcaacc aaaagttaag gagggccgca
    18481 gtagaaaacg gtgcctgaac aacatcatag aatcctttga aatagggaat agttgggtaa
    18541 tcagtaaata cgccatccgt catcgtacgc ttgttgttac taatattaat cgcaaaaatt
    18601 aagacgaaag cgccaaaagt tgagacaaac atcatgatag ttgaaatgag ttttcgtttg
    18661 cgacttctta caatgatttt gcctacccaa gcattgatat aaatagctaa taccatagaa
    18721 ctcactagta acactaaaaa gagcacgata gctactaaaa tagacaaagg atttcccaag
    18781 agttgccaat aggcaattaa gagaagagag ataagaggca tcaaaaagac agcacccatc
    18841 cctagcgacg agacaatttt tgcaatatag agttcttctg acgttactgg taagtgaata
    18901 taaagtttta ggtcattact ttcataaaaa atggtgtaaa gagagctaaa ggctgtcaag
    18961 gttgacatga taaaaaacat agcaacgtca aaggagaaaa gtcctggata atgactgaaa
    19021 tcgacaccaa taaacataaa aaggtaaatc accaaaaaca tggcaatcat taaggcctgt
    19081 tgccttatca tgcttttata ggctttgaag ttttctttcg ggtgtttttc ctgacgcttt
    19141 ttgaggttag ctaaactttg tggattggaa taaagaatgt ttatcttgat aagttcccaa
    19201 atagtagacc aattcatgcc atcacccctc ttcttgcgcc ttacgcccag cgagttcaag
    19261 gtagatgctc tccaagtctt tgtcaggatg gtgttctttc aactcatcaa tcgttccaac
    19321 aaaaatcaat ttcccttttt ttagaatccc gatacgatca cataactgct cagcgactga
    19381 tagcacgtga gtcgaaaata agactgtatg tccagaagct gcatgagctt tcatcatttc
    19441 tttaaggtca aaagaagcct gaggatccaa acctgttaat ggttcatcca aaatccagat
    19501 gttaggattt gatacaagag caccaatcac aatcactttt tggcgcatcc catgtgagaa
    19561 actatcaatg gtttgattaa cttcttcctt cagttcaaaa agtgttgtca gctgtgctaa
    19621 ccgttcttct ctgtcctcat ctgacacccc ataaatttta gctaaaaact gccaatactc
    19681 atttggagtt agattcaaga aaatatcagg agaatctggc acataagcaa tttgtttttt
    19741 gatagcttct ctattttctg tcagaagttg accatccaca aatacttcgc cataactcgc
    19801 ttcgataata gaagtcaaaa tgctgatggt cgtggtttta cctgcaccat tatgtccgat
    19861 aaggccaaaa atttcaccgt cattgatggt gacgtttaaa tcgctgagag cttctttgtc
    19921 gccatacaac tttgaaacat gtttaaattc tatcatggta tcctttttcc ttttcttctc
    19981 tttttctccc tagactaatg aggcttcctc taaaatatac ttttccaatg actgcttaaa
    20041 acggcaaagc gattttggaa gtaaaatgtc attatcaagt aagtaattat ccacatcttg
    20101 gtttagttgg taggcaacaa gaagtgggtt actgtgttta attaatctat tagaagctgc
    20161 taaaagtagc tgcgacaagg ccacattttc acgattttct ggataatcat aagcctgtct
    20221 aatcacatcg tataagcgct gtcgtttctt ttccatttat actcctttat tttttgtact
    20281 tttacgctaa tacaataaca attaaattat aatttttatg aatcgttttg tcaatgactt
    20341 cttatcaaaa gaagtgtatt aggcagtttg aggaaaagaa agaactgggt attttatttt
    20401 aaaaaaacag agtaaatcaa atgatttact ctgtttggga tgtttaactt agagtgattt
    20461 gtataactca gttgcatttg ccacagtgaa accatattct tcgaagatac gatttcctgg
    20521 agctgaagca ccccaagtgt caatggtcaa tgtttttcct gataggccga catattttgc
    20581 ccagccaaag cttgaacctg cttcgatagc gagacgttta gtcacagctg ctggtaggat
    20641 gctttcttta tactctgctg actgttcgtc aaagatattt tgtgatggca tagatacgac
    20701 acgcacatga atgccttctg cttcaagagc ttcttgagta tccattgcca gtttcacttc
    20761 tgaacctgtt gcaatcaaga taccatcaag gtcgcctttg gcttctgaca agatataagc
    20821 tcctttgcta agaccatctt ctgccaattc ttttgtgccc tcaagcactg gaaggttttg
    20881 acgcgtcaat acaagcatgg ttgggcgatc tgtttcagca atggcacgtt tccaagcagc
    20941 atttgtttcg ttaccgtctg ctggacgaat aacgttcaag ttaggcattg agcgaacact
    21001 tgccaattgt tcaattggtt cgtgcgttgg cccatcttca ccgacagcga tagaatcatg
    21061 tgtcatgaca taaactgttg gcaagttttg gagcgctgcc atacgaacag ctggaagaag
    21121 atagtttgag aagacaaaga aggttccgcc gtaaacgcgt gtcccgccgt gaagggcaat
    21181 accgttcatg gctgcagcca tggcaaattc acgaacgcca aaccagatgt tacggccttc
    21241 gtagtgacct ggttggaaat ctgtttcagc cttaaccata gtattgtttg aagctgaaag
    21301 gtctgctgac ccaccccaga aagaggctac ttgttctgaa atttgttgga tggcttgttg
    21361 actggataca cgactcgcca ttgaactacc aagttcgtgc gcttcaagtt ctacttgagc
    21421 agcttcgtta gtaaaggctt tttggtattc tgctgctaat tctggatatt ctgcttcata
    21481 agctgcaaac aagtcattcc aggcttgttc tgctttttca ccacgcgctt gaagaccttg
    21541 agcaaagcgc tctgttactt cagcaggcac ttcaaaatct tgatgagtcc attggtaagc
    21601 ttttttggca aaagcaatgc cttctgctcc aagaggagcg ccgtgaacag ctgaagttcc
    21661 ttgtttttcg gcaccaaaac caataatggt tttgacttcg atgatggttg gtttctcagt
    21721 ttcagcttta gctgcttcaa tagcagctgc aatttcttct aaatcatttc catctttcac
    21781 aaggatatgt tgccaaccat aagcttcgaa acgtcctttc acatcttctg tgaaagccat
    21841 agaggttgga ccatcaagag agatgtcgtt tgaatcatat agcaagacca atttcccaag
    21901 ttttaaatgt cctgccatac ttgctgcttc ttggctgacc ccttccataa ggtcaccgtc
    21961 accattcaaa gcaaatgtgt agtgatcaac gatgtcaaag cctggtttgt taaatttagc
    22021 tgctagatga gcttctgcca tggccatccc aacggcattt gcgatccctt gaccaagagg
    22081 tcctgtggtt gcttcgacac cgtctgtgtg gttcacttct gggtgacctg gtgttttaga
    22141 accccattga cggaagttct ttaaatcttc tacagataaa tcataaccag ctaagtgtaa
    22201 caagctataa agcatggcac ttccatgacc tgctgatagg ataaaacggt ctctgtttga
    22261 ccaattacgg cttgttttgg gattgatgtt catgaagtga ttccaaagaa cataggccat
    22321 aggtgctgcc cccattggaa gtcctgggtg tccagaattt gccgcttgaa tggcatccat
    22381 tgatagggtg cggacagtgt ttactgccaa ctggtcaatt gcatcaaatg tcataagtaa
    22441 aacctctctg tttattacaa ctaaaattaa acgttttcta ttgtctattg tatcacaaaa
    22501 atagacgctt ttttcagaaa atcctccaaa gtccctaaat tctttttctc tattttgcca
    22561 gaaaaaaact agtttggaca tcattgatgg attctttttg acgatgaata taagagagcc
    22621 tgttgacaca gtggccacag actctttagc cttatttttt aaaggaagcc caatcttcca
    22681 taaaggtttt caaaccattt gtcgttaagg gatgttgcgt catcttagca aacaaggggt
    22741 ctggaatcgt cgcaatgtgt gcgcctaatt tagcaacagc ttccacatgg gctgttgtac
    22801 gaatacttgc tgcgatgatt tctgcttgga aatcgtacaa gtcaatgatt tcacgaaggt
    22861 cgctgattaa ttgataagca tctgtgccaa tatcttccaa acgaccgata aatgggctaa
    22921 tgtaagttgc gcctgctttc attgccatta aaccttggct tactgtgaaa atcaaggtca
    22981 cattagtttt aatcccttct tttgaaagaa tgtttgttgc ttttaaccct tctgtagtca
    23041 ttggaatttt aacgacgaca ttgtcatgcc acttagcaat gcttctagct tcttctacca
    23101 tagcatctgc tgttagccct gtcacttcag cactgattgg gccatcgacg atgtcacaga
    23161 tttctttgat aactgtttca aaatcacgtc cttcacgtga gataatggtt ggattggtgg
    23221 tgacaccatc tacaacgcca agttcattaa tcgctttaat agcggctaca ttagctgtat
    23281 ctaggaaaaa tttcatttct tacctccaaa aattttcgat tttgttaagc tctatttgat
    23341 aagctttttc aaatcatcta cctttagact atcctacttt attattataa aagtgtaaca
    23401 gtccttagaa aagaccaagt atcacacagt tttgtgtgca aaactagact tagctgctgg
    23461 gctgagtgag agttcctctt ctcgtgtatt tttttgctgg aaaaggtctt tgagaaaagc
    23521 ttctaaagca gtgagttcat agggggagct atactgtttt aaccggtaat agggtaaatc
    23581 ttgtctcggt aaacagacag tctgataggt aatcagacaa tcataaactt tctgatgatc
    23641 atagggcatt aattgaaaat ccctacgatg ccccaaatga cgtctaatgg ccaattccgc
    23701 aatggctgct tccaccttat tttgccccaa ggcaagccct actaaaagag gctcatggtg
    23761 gcgctcaatg gaaaaaatca acaatgctaa aacgtcgtca ctcaactgct gactgaaatc
    23821 cgtttcctga tccatttgca gcactaaatg gtttatgata acccttaaaa gcgctctttt
    23881 atcttcgcct actaattgat aaaggtattg gctattggtt ggttgactaa ggatagctcc
    23941 tttaaaaaag taagctttgg aaaaaaatat ccccaagcca taaatgattt cctcctttgt
    24001 ctgatggata atcacatgag ccttctttaa aagccataag gcttctgaaa tgtgatcagc
    24061 aataggacct ccaaaaccca aactatactt catgctggca ataggaagta gaggataagc
    24121 atgcaaaaag acaaagaggc tcttagcttc aaaagcatca aattcaaggg catagcgtct
    24181 taagtaatgt aaaagcatgc tttctaagcg cttaaagaaa atattagatg tcataaagtc
    24241 actatctctt aaaaaatgat cattgtagcc tttttgaaag gacattcgcg cttgcgaaat
    24301 cgccaaccaa atcaataatt gttctaaggc ttctgccgat aatgactggc caatcagccg
    24361 ctcaattaag gtagccaaat gcgaagcgtc taactgattg accaaggcgt ctatgccttg
    24421 tctggtcaag gtatggcgga acaattcaaa atagaaataa cgccactgaa gctcacttcc
    24481 cagttgtctg ccttgcgaga gcgccagatc aaattctgat aaaagctggt tgaggtgagc
    24541 taattgtctg tttagagtag cttcactaac catcagttca acggataagt tggtgatgtt
    24601 aaagtgttca tggcagtaca tgtaggtcaa tatctgatag gcaaccgatt ctttgagaag
    24661 cagagctacc aaatcctccc aatggaactg gccattttct tttaagctca gactgtcttt
    24721 ttctaaaagt aattgacagg ctaaaccttg ttctcgacac aaatgattta gattgtcaat
    24781 gtattttaat agggtcactt tagacaagtt tgatagcctc attaggtctt ttagccttaa
    24841 cgtttcttta gcatgataaa gggtcaccaa gagacgatat tgggcccttc gttccttatc
    24901 cattaagtct tcaatcttca tggcggagcc tcttttcaaa taatttgaga caagcttcat
    24961 gaagatgata agccaaatca ctgtgacctt gtaaaaagag aaaatcttga aatgctaagt
    25021 cagcgtctgt aactttcaga gccagagcct gacctaattg attggcgatg acaagagcat
    25081 tggttttgga taggagctga gcgcctatta aaatcccatc cgtttttcta gctattaatt
    25141 tcagccaaaa gaggttttta gaatccccat tttggtacaa atagctacac gtatccatat
    25201 aaagctctgc ttcttcttct gtcacaccaa cactcgcgcg ataataatca aagtgagaac
    25261 ttcctaatac cttgaccgtc tgtaatggag gaataggata tccagctaaa tgcagagcca
    25321 gagcctgacc tgtcttcatt gcttggttaa tcaaaggtgt atagtaatcc attcccaata
    25381 taccaaaatg caatgaaacc atgtctccaa tggcatagat attagcctgg ctggtctgta
    25441 agttttcatt aaccaaaatg gtcttatcca gagcacaagc cacttgtcct tgcaaaaggt
    25501 gtgtatttgg cctaaagtta atggctaaaa tagccaaatc accttggaag gtgtcccctt
    25561 gctcggtatg agcgacaact tttccctcta tctcatgaat agccgtaaca cgcttattat
    25621 taataaaggt gacatggtga gtggacattt ctgccattac aggggctatc agttctgcgt
    25681 cagtctgata gaaatctgga cgttcggctg cttcaatcag tttgacccgt ttgccttgta
    25741 aggacaaact gtaggcaagg tctaagccaa tcaagccggc accaataacc aagacctctt
    25801 ggctagcctc aatcagtttt aaactagctt ggctttcttc ataatatttg gttaccaaaa
    25861 ctttgttggt ctgtgacgtc tcaatataat gagactgggg gctagcaccc atggcacaaa
    25921 caagcgtttc ataaccttct tcaaaaactc gtccttgact gtctttgaga agcaaggtgt
    25981 ttgagggagc ttcgatagct aacacttctg cttgaataaa ccgatggtta gactctattt
    26041 gtgctgctag gcaggctctt ccccacatag catcagacag gtcttgaatg tctcctctga
    26101 ataattggtt gataccattt ggaatataat tgggacagct ttccttatca atgagaatga
    26161 tttgggaatc tggattgagg tccttatatt tatcaacaaa ggctagacca gcaaaggatg
    26221 ccccgataac atgtatggtc tttgtcgtta atgtcataat agtattatta tagcagtttt
    26281 ttttaagata tgaaagcgtt ttacctaatc ctataaagaa aatgcaaagg agctggccta
    26341 tgatagccaa cccctttagt gatggttagg acatgacttg gaaaagcaaa actgctaaga
    26401 ccgctccaat aattggtcct acaacaggta tccaagcata agaccaatca gaatctccct
    26461 tgttgttgag tggtagaata gcatgaacta gacgaggtcc taaatcacgt gctgggttaa
    26521 tggcgtaacc tgtcgttcct cctaaggata acccaatccc aataaccaag gtacctacac
    26581 agagggttcc tagtcctgct ggcatatcat ataagccaaa tgccaaaata ccaagcatca
    26641 agacaaaggt cccaaagatt tcactcaata ggtttgagct agtatctcga atagcaggac
    26701 ctgtcgcaaa tgtccctaaa atatcagcct gactctcagc agccagataa tgtggcctga
    26761 actgaaggaa taccagagtt gaaccaacca tagctcccaa tagttgggca aggctatagg
    26821 caatggctgt tgaccaggca atggttccac tcatggcaaa agcaaggcta acagctggat
    26881 tgaggtgggc aggggcaact ttgccactga taaaggctgc cacagcaact gcaattcccc
    26941 agccagtcgc aatcacgatc cagccagaag catgggtttt cgttttgggc aagacaaccc
    27001 ctgctaccac accatttcct agtaaaacca ataaggctgt acccaaaaat tcgccaaaga
    27061 tatccatatt aaccctcctg atggcctttt aaggcactca aatcattgtc tgcaatcacg
    27121 cgacgaagat cgtcttcttg tgccactctt tcctgatcag accattcaaa atgtttagcc
    27181 atttcattaa tcactgggtc aatcaaagca tctaggctat ctcgcataaa taagaggtga
    27241 tttgttcttc tcaagaaata atctgtcggt ttaagagcca tttcataatc catcgcataa
    27301 tgtaaggaca aggtttcagc taaacttaac ccttcagctg ctgttaattg acgagttaag
    27361 gcaaagactt ttggcgcatt agaaccataa aggtttgcca aatacctagc atcatccatc
    27421 gaaagaccac taagagttcc taattgagca taggcttcta tttccgaatc tacattggct
    27481 ggattgattt cacctcctga aacaggatag gtttttgaat tgataagctt gaaggatttg
    27541 ccaaactctt ctttgaggat ttgaataatc cctgtcaatg ctccttcagc cattttgcga
    27601 tagtcggtaa tcttaccacc tgccaaggta aagagtccat tctcatcacg ctcaaaactt
    27661 gaacctcgtg acactgcaga cggatccaat tctttttcag atgtgctggt ttcaacctgc
    27721 ttaatagctt tttcaacagc ttctcggcta tcttcgtggt taatataggc tttgacagta
    27781 tcaaccaagt gatcaaaact atcatcactg actttaccgc tgttgccacc attgtagtca
    27841 gaagcactat tacctgataa caaggggcga agaccagccc aactgctttc aatatcatca
    27901 atggtcacgt tggcatttgg aaagcggtta ttgacaacgc ctaacaaata atccacatct
    27961 tcttgagtaa cttgtgggtg ctccaagtct ccagtgtagt ccgtgtccgt tgttccaaaa
    28021 taagtttttt cctcacgtgg caagacaaag accatacgac catcatttaa ccctgtgtca
    28081 acatagacag gttgagacac tggtaatttt tgacggtcca ctaccagatg cacccctttt
    28141 gtagggcgca tttgatgaat cggttgtccc ttatgagaga attgacgaat ctcatcactc
    28201 catggacctg tggtgttgat gactaattta gccttgataa tgatttcttg atctgacagc
    28261 agatcacgcg ccttcacacc aataatctta ccattgtcat ctagcaagaa atcttctgct
    28321 ttcacatgac tagcaataag agccccatca cgattagctc gtttgatatt ttcaataaca
    28381 agccttgcgt cattattgcg gaaatcaagg taaaccccac caccaagcaa gccttcttgt
    28441 tttaagtctg gttctcgttt taagacttct tctttggtta acaccttgtt ggccgctggc
    28501 gtattggaca cgcctgctaa aagatcatac aaatccatag cgaccttcaa acggaacatg
    28561 ctaaaggtac tgccaggttc gtcataaaca ggtaataaca tagggtctgg ttttggaatg
    28621 tggggtgcaa tttgttgcac cacagcccgc tctgacaccg tatctgaaac cacctccaca
    28681 tcaaattgtt tcaagtaacg aagccccccg tgaaccaatt tggttgaacg gctagaggtt
    28741 ccttgagcaa aatcttgcat ctcaatcaga cccgtatcta ggccactagc tgccgcctga
    28801 agtgccacac cagcacccgt aatacctccc ccaataatca gtaggtctaa atctctttct
    28861 tgcatttttt ggagagctaa gcgtcttgtt tctcttgaaa attccattcc atcctcctct
    28921 agttattttg catcgtcatc tgcatcttct tcttgggtga aaacttgagt tgctttaaca
    28981 gcgcgcttcc aacccttgta taatttttct ttgcgcgatt cattcatgct agccttaaag
    29041 agctggcctg tggcatttaa ttctttcaaa gcatccatat cttcccaata ccccacagca
    29101 agccctgcta agaaagctgc tcctagagct gttgtttcta aatttttagc acgggcaata
    29161 tcaattccta aaatatcagc ctgaaattgc atgagcatat tattcatggc tgcaccgcca
    29221 tccacacgga gctgttgaat gtcaatacct gaatccacct gcatggtatc aataacatct
    29281 cgcacttgat aggcgataga ttgtaaggtt gctttgacaa agtcttcttt gctggtacca
    29341 cgtgtcaagc caaagacaga tccacgcgca ttagaatccc agtaaggagc tcctaaaccg
    29401 gtaaaagcag gcacaacata aacttcatcg tcactagttg atgctagagc gaactgttca
    29461 gattctggtg aagtctcaat cattttaaga ccatctcgca accattgaat ggcagagcct
    29521 gcaataaaga tggaaccttc caaggcatag tgaaccttcc cattaatccc gtaaccaatg
    29581 gtggtcaaaa gattattact cgataactgc atctcatcac cagtattcat gataatgaaa
    29641 gaacctgtgc cataagtgtt tttcaccata ccaggctcaa aagctagttg gccaaacaaa
    29701 gctgcctgtt ggtcacccgc catacctgaa ataggcactt cgcccccata aaaatgaaag
    29761 gcagctgttt tgccataaat ttcagaattg gatttaacct ctggaagcat atcttttgga
    29821 atatttaaaa gctctaaaat ctcatcatcc catgttaagt ctttaatatt atagagcatg
    29881 gtacgagccg cattggaata atccgtcaca tgaacagcac catctgtcaa tttccaaacc
    29941 agccaagtat caatggttcc aaaaagtaac tcgccttttt cagctcgttc ttgagcacca
    30001 ggaacatggt ccaaaatcca acggatcttg gttgcagaaa agtaagcatc aataacaagg
    30061 cctgtctttt catggatcat ttttgtatgg ccatcttgtt tgagttgctc agcaattggt
    30121 gccgtctgcc gtgattgcca cacaatagca ttataaatag gtacacccgt ttttttgtcc
    30181 caaaccactg tggtctcacg ttggttggta attccgattg cttcaatttg gcttggtttg
    30241 atgctagact caataaaagc acccgcaata acagactgaa ccgaattcca aatctgatta
    30301 gcattgtgct ctacccaacc agcgtgtggg aaaatctgag ggaattcttt ttggctactg
    30361 ctaacctttt cccctttttg attgaaaata atcgctcgtg aacttgttgt tccctgatca
    30421 atagccatga tatacttttc ttgcgacatt cattaccctc ctttaagtaa gcgttttcct
    30481 atttgattct attatagcta attttatcag aaatatatta tcaagaattt ttaaagctaa
    30541 aaaaaaagct gataagatag gtagcttatc ccactagatg aacttgaaaa atccactgat
    30601 gacaaccacg tctcctcatt tctaacgagg taatctttct tatcaaagaa aatcaaaaga
    30661 cccaacaatc actctgctaa tccagagtca tcattgggtc ataaagagaa ctaagcctca
    30721 aaggcaacac ggtatcaaac agccgcgcct aagaggcaaa gacaattgga cacaagagtt
    30781 aaggctgtct tattcattac agtccttgat tagtcattga ttggttcaga agatttttcc
    30841 acaatatccc ctgagttggc gtcaatggta tagctgtatt cttgatcttt gttttgaaac
    30901 tcgatttcat acacggcttt gccatcttca ttatcttgtg taactgatag catttgagca
    30961 tcagcttccg taacagaagc atcttttaac gcaattgatt tggctttgtc ttctgagagt
    31021 ttaactttct tgctagtttg acttgtcttt ggttgattat ccatatgatc gtctttatca
    31081 tccatatcac aggctgcaag actgagtaaa gagaggctgg ctatggctag tagagagaat
    31141 tttttaattg tcataagtac taccttcttt ctttgatttc ttctattata actcaaacac
    31201 atggtttttc ccaatatttt gactatacaa tataaaaacg tgtggtcact ttgaagtatc
    31261 ataccattta aaataaaagc taacagtcat cacgataaac aaaaagcagt tcagagctgc
    31321 tttttgtctt atgattttgg gtcatctaat gaacggccgt gcaagccttt ttcacgttgc
    31381 acctgtctta atttttcagg ggtcacgtcg tttccttctt cgtccaccaa tttgatgcct
    31441 tcgatatggt gtctgacaga gcgacgatag ccttcaatgt attcttcacg gagttttgcc
    31501 tgttccactt tttcaggtcc agtcaagcct accgtttttt tctttttagc cagctcattg
    31561 atacgagcaa tttttttagg atccatgtta agccactaag agcctgggaa aaaagtataa
    31621 ggcttaaaaa actgacttga ctctcactgt gaaaagcaat gtttaaaggc cttgtttttt
    31681 ccactcgggt actgaactca cttttctagc taagtcccca acggctcttt cctttctatt
    31741 tacttggtgt ttagttggtt aaaagcaaca atggtcttag ctttgcttac taaatctgat
    31801 aaaatagcta aacggttgtt tttaagggct tgatctcctg ccataaccat tgtgttatca
    31861 aagaaatcat taatcactgg gctaagggca aagacctgtt ctaactgctg ggcagcactg
    31921 cctgctagag tcaagccttg aatagctgca aacaaagtgt tttcttgctc attttcaaag
    31981 aggctagggt ccacctgaac agaagcgtcc gctttttctg ccaaattaaa agcacgtgac
    32041 aaagattcaa ctgctggttt gtaattttcc gtatgactgg ctttgacaag agcttcagct
    32101 gcggccaaca tttctggaac cacaaaggtt gaacttgcta gaatagcttc ccgaatgtct
    32161 tttggtgcgg ctttgcccat catcttatcc acacgagcac ggataaagtt catcacatcc
    32221 gccttattgg cataggtcaa actatcaaag gacaagtcat ataagctatc taccagctta
    32281 tccattggga tgcgccatcc aaaatgatca agaatacgga caatcccttg ggttgcacga
    32341 cgtagagcat aaggatcatt agagccagat ggaatcaagc caacagaaaa gaatgacaag
    32401 agagtatcca atttagctgc caaggccagg acagcgccaa ctttggtttc aggtagcgca
    32461 cctcctgcag catcaggtaa gtagtgttca cggattgctg ttgccacagc agcatcttca
    32521 ccagctagaa gggcatattt ttcacccata atcccttgca gctcatcaaa ttcacccacc
    32581 atacctgtta acaggtcaaa tttgtaaatt tgggcagcac ggtcaacagc tgtgacttcc
    32641 tcagcagata ggttggcttc cttagcaagt gatgcggcaa tgacacgcgt acgatccatg
    32701 tgttcagcaa gagagccgat tttttcatgg aaggtcacat tggtcaattt ggcaaccaaa
    32761 tcagcaattt gcaatttctg atcctcacgc cagaagaatt caccatcttc taagcgagct
    32821 accaaaactt tttcgttccc tttaataaca ttttcaatgg cttgatcatt cccattacga
    32881 actgatacaa agtttggcat caagtgacca gcctgatcac gtactacaaa ataacgttgg
    32941 tggtttttca ttgatgttac caatacttct tctgggacat caaggtattt agcctcaaaa
    33001 cttcccatga aagctgttgg aaactcgacc aagttcaaaa cttcattaag caagtcttcg
    33061 tcaatgtcaa cttgcacacc ctgctccact tcaagcgtct tgatttgctc cacaatcatc
    33121 tcttgacgct ctttagcatc agcgataaca aactggctgc gtaaatcagc ctcataagaa
    33181 tcagcacttg tgatagtggt ttcagtacct aaaaaacggt gaccacggct gacgcgtcca
    33241 gaatgaatat ccaagaagtc aagttccaag gcctcatcat tcaacagaac tgtcaaggtg
    33301 tgaacaggac gaatgtattc aaaactattg ttagcccagt gcatgctgac tgggaaggtc
    33361 attgctgata acacttcagt aacgcctaga agcacttcct tagcaggttt tccagcttcg
    33421 tgtttggtca cataaacgta ttcttctcct ttgacttcgc ggaattcgat agcatctgtg
    33481 gtcaagcctt tgccacgaac aaatccttga gcagcttttg agaaattccc atcagcatca
    33541 agggcaattt tcttagcagg ccctttaaag tcctcagtca aatccgtttg ctgatcagct
    33601 aatccagaca cacgcactgc caaacggcgt ggagttgaaa aggtttgtat atcttcaaaa
    33661 gagagacgat tttctgttaa gaaagtcgcc aagcgctctc ccagttgttt ttcacttggt
    33721 gtgaccacgt aggctggaag ttcttccaga ccaagttcga ttagtagatt tttactcata
    33781 attaagatac caagggcgtt aagcggacac catcaaaata gcaaagcgac gcaggtgctt
    33841 gcacctagga agtcttggtc tttttgacat agtccgtagc ccgtgttcag ttcctttctc
    33901 taaaaagtat ctttttgatt gttcccttag gtagggggga cgcaaagtga aattatccag
    33961 tggataattt cagaaaacaa aagtgttcca ttcctagtta gccagcctaa agtctagcca
    34021 actccctcgt tcgataagta accttaccga attgtcacta cagcgggagt aaccttgact
    34081 cggcgacctt cggtcgccca cttcttgctc gctcttaaga ttacaaagct cgcaactaga
    34141 tcatctttaa atcgacttat tcgtcatctt cagctagcaa gattgcgcgt gttgcttcat
    34201 ccaacagtgg aaatccaagc ttcttgcgtt ctgcaacaaa ggtcttagca acgacacgtg
    34261 ccaaattacg gatacgagca atgtagcctg cacgttctgt gacggaaaca gcgccacggg
    34321 catccagcag gttaaacgta tgagaacatt tgagcacgta atcataagct gggtgaacca
    34381 aaccttcttc tagggcacgc gaggcttctt tttcaaactt ttcaaagttt tcaagcagca
    34441 tgtcttggtc tgatatttcg aaggaatatt ttgaatgctc atactcaggt tgtaagaaga
    34501 tttcaccata tttaacacct ggtgcccact caatatcata gaccgaatcc acttcttgaa
    34561 tataggaagc taagcgctca agaccataag ttacttctgc tgtcactgga gatgtcgcaa
    34621 ggccaccaac ttgttggaaa tacgtaaatt gtgtgatttc cattccatca agccaaactt
    34681 cccaaccaag ccctgctgat cctgttgatg ggttttccca gttatcttca acaaaacgaa
    34741 tatcatgttc taaaggattg atgcccaatt tttccaaaga agcaaggtaa agttcttgaa
    34801 tatttgatgg tgatggtttc atgaccactt gaaattggtg gtgttggtaa agacgatttg
    34861 ggttttctcc gtaacggcca tctgcaggac gacgtgatgg ctctacataa gccgcattcc
    34921 atggctcagg acctatagca cgtaagaacg tgtaaggact catggtccca gctccttttt
    34981 cattgtcata ggcttgcatg agcatacaac cctgatcatt ccaatattgt tgtaaggtca
    35041 agatgatttc ttgaaaggta agttttttag acatttcgta ctcctttgat agtagtaaaa
    35101 tggtatatca ttgacaatcg tttttttgtc atcagtaaaa tacctgacga tgttactagc
    35161 atgtattaca ggtctaggca ccaaaaaacc gcattttgat gctcctaaat cataagacct
    35221 aggggcgtca aaacgcggtt ccaccctaat ttattacttc atttatatag tatctttttg
    35281 ggcaatattg acaaatatac ttaatcacac tgcccaaacg gaagcgccaa tctgcagtcc
    35341 taccattcgg ctctaactac cccgaactcg ctttagataa gatacttgca tcctttttcc
    35401 tagagtctag tttagcataa agccattagt ttgtccatca tgagcttaag gttttgttcg
    35461 agtaccgttt ggaataatca cttccgctaa tagcttgttt tcttctcgaa tacgtaaata
    35521 tattactaca atgtagcatg ggaaaagaag catagccgta attttagcat ggcaaagtaa
    35581 ggctatgccc accagctcag gggcgatatt taaaaagtag ttaggatgtt taatcgtttt
    35641 gaacaaccag tgatccacat atttgtggtc ttttgctaac atcaatttta cagtccaaat
    35701 gtcacctaag atacgtgtca cttcatataa aacagctact gataataaca tcaacaaaag
    35761 acctaaaaaa ctaatgccat caaacttaat ttgtttaagc agcgcttcaa tcaccgcaaa
    35821 cacataaatc ataatgtgaa gtaacgtcaa aaatttcgtg ttctgagcac caaattcctg
    35881 tcctccttgg gctaatatgg ctttttcatt tgttatcgat ttcttcaaaa atactaaacg
    35941 aatagtaaac atagctaaca tcactaaaaa gatggtcaca atacggctcc ttattttcaa
    36001 taatagtttc tttcacaaaa cactaataaa gattatagct aaatgataaa cctttgtcaa
    36061 accgttttca aaaaagattt agcgaattat ggctaaatct ttgtagttcc ttaaaaactt
    36121 gtagtatcag gggtaggaat cgctgacatt cctgagagat agcgaattgt ttttttatct
    36181 tcttgtgtca agctcacatc aaaaatagcc atattttcct tgatgcgctc atcatgcacc
    36241 gattttggca agggaataaa accttctgcc agcgaccaag ctaaggcaac ttgtgccact
    36301 gttttgtcat atttattagc taactgctgc atagtctcat tatcaaaaat ctccccttgt
    36361 cctagaggac tccaagcttc caataaaatc tcatttgcct tacaataatc aaccacttcc
    36421 ttttgatagc aacctggggc taatcgaatc tgattaattg ctggagttat tttagctgtc
    36481 tcttgaagag cttctagatg atgcaccata aaattgctga cgccaatgct tttaataagc
    36541 cctgcttcaa ccgcctcttc catatattgc catgcttggg catttgcctc tttccaagtg
    36601 ttacgcagag cttttgggtt tggccaatgg ataagataga gatccacata atccagacca
    36661 agacggtcaa gagaagcagc taaagcgtct tttgcccctt cataactatg ggcgtcattc
    36721 cagagtttag ttgtaataaa taagtcttct cgtagaaccc cagaatcctt gatggcacga
    36781 cccacacttt cttcattttt ataaatagct gcagtatcaa tatgacgata gccagccttg
    36841 atggctgcta aggtagattg gtaagcttcc tcaccatcgg ctgcttggta agttccaaat
    36901 cctaaaacgg gaatctcata gccacttgtc atttttactg ttgttaccat cacaaaaacc
    36961 tccttttatc aatatctgta agcgattact ttaatctatt ctatcataaa gaaagaaaat
    37021 acaaaaagaa ttggcctgcc agacatagct accccaatcc ttttgtatct cttaggcatc
    37081 aaaagctttt tgcccattta aataggttgc aacaagtgtt aaatccttat caagaacaat
    37141 aaagtcagcc gcatgcccag ctttgatttg accacatacg tcgtcaatac cgacagaaac
    37201 agctggcaca taagttgcca tatgaatggc ttctgccgga gttgcgattc cccaggccac
    37261 tacatttcta aggccatctt ttaatttcaa aatagatcct gccaagtttc cactctcttt
    37321 aagacgagca gtgccatttg ccacaaccac agagaattcc cccaagaggt aatcaccatc
    37381 tggtgaacca cctgctcgca tacagtccgt aatcatggct acatgatcat gccctttttg
    37441 ctgcatcaaa atatcacaag caattggtga gacatggtgg ccatcacaaa tcaactctgc
    37501 gtaagtattt ggaagattat aaaccgctcc caccattcct ggttcacggt gggtcaatcc
    37561 tcgcatgcca ttataagcat gaacccaaac actagcacca gcttgaacag cttctttagc
    37621 ctcttgataa gtaccgtttg aatgccctaa ggccactgtt acgccttgtt ttgtcactgc
    37681 agacacaaat tctttgacac catcacgttc aggagcaagg gcaatttttt tgatgagccc
    37741 tttagcagct ttttgccaag catcaaattc ctccaagcgt ggggttttca tataacttgg
    37801 gttttgagca cccttatatt cttcggtaaa ataaggacct tcaaagtaga taccttgtat
    37861 tttagcacct tttacctgat cagcaactga agcaatagtc cctgatactt tttctaactg
    37921 ctcaaaggta gaagttaagg tcgtcggtaa aaaagacgtt actcctgttg ctaatagtcc
    37981 ttcactcatt tgatgaatcc cttgggcaga attatccatg acatcagctc ccgcatagcc
    38041 atggatatgc gtatccacaa gacctggtgc aatttgataa ccggtgtagt caatgatttg
    38101 cgcatctgcc ggaacaattt ctgtccactc cccaaaaaca ccatcatgaa gacttaggta
    38161 gccagccggt ctcacctctg ttgggtaata aaaacaatct gctttaagat agcatgtcat
    38221 cttttttcct cccttttttc cttattatac gccttattct ttactttgta aatggtatat
    38281 accacttttg ggattcaaaa aagaaaacgt ggatgatcat tcacgttttc tcttttttta
    38341 aagctggtgt gaaagtactt tctctgctaa attcaaggca tgatctgtga tgcgggtata
    38401 gtgggaaata atatcaataa aattaatacc tgcttgtgcc gtacattctc cacaattcaa
    38461 gcgcttaatg tgggttttac gtaatcgtcg ctctttttct tcaatttctt tatggcgagt
    38521 cacaatagtt tgcgccaagt ctgtatcact atcaacgata gctctaatgg catccagtgt
    38581 taaacaatgc gtcaattgat acatctctgt caattcttga cgagcagata ttgaaaaacc
    38641 aatttgttta gatatgatgc cttcaattag aatgcctaaa gactcagaat gatctccaat
    38701 tctttccaaa tcacgtgaag agtcaagaat accagccaag acttcatttt cacttgggct
    38761 gagggcttcg ttagaaatat caaccaaata agtggttaac tcctcatcga tggtatcaac
    38821 tgctctttca taacgcttga ccttttcccc aaatttaccg tcagctgtca taatataact
    38881 gtaagaagct tcaaaagctt gaatcgcata agaggctaaa tgaaccaatt ccttgtgggc
    38941 atttcctagt gcgatagaag gggcttgggt aataagtaag cgatctaaat aaagggcttc
    39001 gtatttgacc acttcatctt ctcctggaat gagtctagtc acaatcatag ctagtagact
    39061 aatgaaagga attaataaga tggtattagt gatattaaaa gaaccgtgag aaaaagcgat
    39121 agtcatctca ggcgttaaac tcaactttga ttgtagccat aacatcaatg atgtgaatgg
    39181 gactaggata atcataaaaa taattgtacc gatcaaatta aataacacat gagctgctgc
    39241 cacacgttta gcagcaatat ttgaacctat agctgctaag acagctgtaa tgcaggttcc
    39301 tatatttgag cctaacaaaa taggaatagc cccctgtaag gtcaaaagac cgcctgaaaa
    39361 cagtccctgt aaaatcccga taatggctgc cgatgactga atcaacatgg tcaaagccgt
    39421 tccgataaag acaccttgga agggtttatc acctaatgtc gctaaataat tttgaaaggc
    39481 tgacacagat ttcaaaggat ccatggcatc tcccattagg ttcagcgaga agaaaattcc
    39541 tccgacacca aaaatgatac gcccaaaatt gttgagcttt ttattagagg taaagaaaag
    39601 acaggctgca ccaataaaaa tcatgggcaa ggcgtaatct cctagtttaa aaccaattaa
    39661 aaacgaagtg atagtcgttc caatattagc ccccataaca attccaatgg cttgtcgcaa
    39721 atttagcaaa cccgcagaaa ccaaaccaac cgtaattaca gttacgcctg aactggactg
    39781 aattaaggca gacatggcaa ttccgactaa gataccaaaa aagggattac tggtgtattt
    39841 atcaatgtaa taccttagtt tatctcctgc tgcttgttgc agaccatctc ccatatactt
    39901 gatactgaaa agaaagagac ctaagcctcc aaagaaatga aatgcaatgt cttgccaatt
    39961 tactgacaca atattgtcct cgctcaattt tttagtctat tctattttaa tgtattacgg
    40021 tctttatcac aaggtttttt taagcgtttc tgtgtatacc gatgtcaaca tttgcatatt
    40081 attggtttga taaggtgtta ggcttttccg atattttaca tcatttgttt ttgcacaaat
    40141 cttttcattt cgtttaatta gaggtataat atggctacga tgaaacaata tcataaagga
    40201 gattccatga ctaaatcaca aaaagaggcg ctttattgga tgttgagtgt tctaactatc
    40261 actctaatag gtggctcctg ccttattttt ggatcccacc ctcaaacgca agataaggta
    40321 gctaaacatt ctaaaagtgc tgctagttta ctaaaaaaag cagttaaagc agttaatgac
    40381 gctgatcgtt tagccactgc agctgctatc caagaagccc agaaagctgt tgataaatta
    40441 gcagaatctt ctaagaaaaa aacgctacaa gaacagctaa acgttgccaa agcaaagcaa
    40501 gagcaagaag atgcagctac tcaagcagtt aaagctgctg aggagactct caatcaaaat
    40561 cttaaagata tcgcccaaaa agcagttaac gacttaagta acaaaggtaa aaaagccgct
    40621 ttacaatctc ggttagacgc tattttacca gcgaaaccca ttattgatga atttccaaga
    40681 caaagtggag aaataacaga taactcttac tggacacctt tcccaggaga cgtctcagat
    40741 acttatgata attcccagtc acctacttta gacccaagct cagaatcttc agcttcagat
    40801 gttacaccac aacctagtca tcctgatcca attccacctc agacctcttc agaaccttcc
    40861 gactcaggag acaagcaatc atcaaaagag taatacaaca gtttttcgta aaatcaatca
    40921 ataaacgttt tagacggcta tcttagtgat tagtcttcct gacatcttaa aaaagctagc
    40981 tcaaatcaaa agagtctagc tttttgtttt gttagatagc attaaatttc gtagcccatt
    41041 aaaagtcctc cgtcttcagc ataaaagcta cacaaacctg aggtccttcc agtttgaatc
    41101 acagcatcag gatataaaga tttgattttt tcactgatct gctcacaaat ttttggattt
    41161 tgagcatgag caatgtaaac ttttccgcct acataaccct ctttttgaat ttcttctatc
    41221 aaggcagaaa ctgctttttt ttgaccacgc gctttttgaa gaagttcaag cgttccttta
    41281 ttgctagctt tcccaaccat tctaatattg agtaagccaa ttactttacc tactaattta
    41341 ctcagtcgac catttttaac caaattatca accttggcaa gcacaaaaat caaacgtgtt
    41401 ttttgctgat aagctgtgat ctgtttgaca acttcttcaa aggataatcc aagtttgatc
    41461 aaccgctcaa gctccagtac gagcaaatcc atctcgccac ctgctgataa agaatcgatt
    41521 agatgaatat tgacattagg attttcttca agaagttcat ttttagccag tcgtgcacta
    41581 ttatgactgc cagataaggt ccctgtaatt gtcatcacga taacattatc tgcacctcta
    41641 taggcctgca aaaaggcttc tgggcttgga cagctagatg tcgttgcttt agacgactgg
    41701 tacatagttg tcatcatatt atcgatatct aatccatcat catctctaaa aatttctgtg
    41761 ccaatctgta gggtcaaagg cacacgttca aagcgcaact ctttggattg tctagtcaaa
    41821 cttctaagat cacatcctga atctgttaca attttccaag tcatttttta tccttctttt
    41881 tctctaattt gtgttcggta acaaagacca gattattaag ccttcgctaa gatgtctagg
    41941 ctaatggtaa taactagtca ttaaatccta tgttttatta ctagcctttt caatagttag
    42001 taaagtcatc caatagagct atacaaggag gtaatgaggc tcctgataac taactttagt
    42061 tgacaaatta agacttttcc cataaaatta tatcattatt aagtcggttt ttgctactga
    42121 taactcatca gatgtaacac ttgaaaggta tttgtgatga ctgataaagc actctcaaaa
    42181 agatcacttc aaaacttaac acaatttaat aaagaaacac gacgcatagc acgtgaatct
    42241 atggaaatcg ccttactaaa tcttctagaa acaaaacctt taggtgatat tacgatttca
    42301 gaattggtga cgaaagcagg agtatctcgt aacgcttttt atcgtaatta tacctctaaa
    42361 gaagccatta tagaacaact gttagtaggg gttatccgtc gtatttttcg tggactaaaa
    42421 caatttgata ttaaaactca ggcttatcaa gcttggctct atctttttac tgaagctaaa
    42481 aaagaagctc agcttcttaa aatgattttc aaacatcacc tgcaccatct tctaacacga
    42541 ttagtcacta aacgtctgaa agcttatcaa aaattaaaag acaaaaaaca atcgcactat
    42601 aagcgccttt tttggagcaa tgctattgtt tctgtcttga caaactggat ttcagatgac
    42661 atgatcgttc ctgccgaaga aatggctgct atgggcttgc ctctattaac gtaaaaaggt
    42721 tccttttggg aaccttttta cgttaattgt tagtcttttt gagctacaag tgcaaactga
    42781 cggatagcct gggccacacc cgccatatca ttagtcaaag tgactttgtc agctaatggc
    42841 ttgatagctg ctgaagcatt ttccatagca actcctaaac cagcataagt taacatctca
    42901 atgtcattgg gagcatcccc aatagccatc acttggtcag cagttaaacc caaatcttct
    42961 actaattctt tgagagccga tgccttgctg actccctgag ggagaatttc taaaatatta
    43021 tcttggctac gaacaacatg aaaactctga ctaagctgat ttctcaccgc acgttcaaag
    43081 gcatctagcg cagctttttc tcctaagtac atggcttgaa aaatcaggcg aggagtgtcc
    43141 gacagctctg ctaaggagac aggcttgaca atggtaaata ccaaatctcc atcttcctgc
    43201 acaaggtctg gcacttcttc ttcaagcacc aaataatcgt tttcctcagt taaagtcagg
    43261 taaatatctg gaaaactttg gcttagctct tccagcaatt caatatctga aaccttcaac
    43321 atttttgaat ggcgtaattg ccagtcagga ctactataag tactacagcc attattgata
    43381 atgaggaatt cctcttgtgt caaacctaat tggtcaaaat agggtcttgt ccctgactga
    43441 ggccggcctg tgcataagac aatttttaag cctgactgag ctgcttcttg gatggctgtg
    43501 atattctctt ttggaatctg tttgtcctgg tttaataagg tgccgtccaa atcaattgca
    43561 attagttgaa tcatcctatc tttcctcttt ccatttgatt ttagggaggt tccatttttt
    43621 acggttgcca atcatgcgta aagtcgtgag taaaacaacc aaggcataac attcaatcgt
    43681 tgaatctgct agtttaaaat gcaaaacaat agctgcaaga atagaccacc cagcatagat
    43741 ttcactacgc aaaacactag gttttcgccc tgccagaata tctctgacaa caccaccgcc
    43801 tgcaccagtc aacacagctg ctacaatgac agcacttaaa ggctggttaa gacggacagc
    43861 atgcaaggct ccttggacac taaaagctgc cagaccaatg gcatcagtca aaacagctgc
    43921 ccgtacccaa cctctagcca ttagcttagg aaaaagcata atcaaaacca tagcaaagaa
    43981 agcgcaagtg aattctggct tttgccccca taaggcctct ataggtaaac caattagggt
    44041 gtttcgaatg gctccgcctc caaaggctgt cacgaaacct aaaataaaaa tccctaaaat
    44101 atcaaattct tcttccatgg caacaatagc accagaaaga gcaaaggcaa tggtcccgat
    44161 gatatttaaa atttcccaca tatcaattgt cataagctac cctattgtct tttagtcttc
    44221 ttttagttta ccacgttttc ctttaaaaaa taagtagaaa aagataaaat gtaaggataa
    44281 aaaagcaagg ataaaaccca ttttcatcct tgctacaaaa gattcaaaca attatcccca
    44341 tacttatctt atcacatcaa tgagagctgc ttgataggat aaggttgttt tagatttttt
    44401 ccgtccaagg gctagcggtc tcttcgagta ccctgtttaa ctcgtcaata ttttggaagc
    44461 cttcagtgca tagccacttt cttgcttcgt cctctccttt agtaatataa actggaacgg
    44521 atcctgccca cgtggcacgc ccacacaaaa caccactaaa ttgagcacct gctgctgctg
    44581 caaagtataa tgtctcttga aaaagtttgg cagatacgcc tgcactcaaa tagatataag
    44641 ggaggtggct ggcagcttct tggtcttgaa aagctttaat agcctcagct tgactgtaat
    44701 ggacttcccc ttcagtaaaa ccttctacat aagccatatt aacaggaacc tctactttta
    44761 gcacatcaac gccaaaacgc ttatccgaaa atactgacat agcaccgtta accttatgtg
    44821 gcttaagctt tgcgtatgcc gcactgttgt tatctgatat tctctcatca taactcaaaa
    44881 gttcaagaaa aaaagggatg tcttcagctg tacattctga accaatgcgt tcaatataag
    44941 cttgtttttg taaattgatt tgttcatctc catcaacatc ataatagagc aaaaatttaa
    45001 tagcatctgc acctgctgct ttcaaccgtt tgaccgacca ttccactaag caatcgggta
    45061 aacggctagt ggttgtagca tcataacctg ttttttcgta agctaacagt aagcctgtct
    45121 gattagctct taccttagta gccagcaacc catattctgg atctaataaa atagatgaag
    45181 cataaggcgt caattcctca gaaacgagaa ctttcaatgt ttctatctgt gtaactgtcg
    45241 cttctccttc ttgatgtgct gccatcattt gcttcagtgc acctcgttga tcaaaagcta
    45301 aagctgatat gattccttga tggctcactt tttctaagta atgtctttta tttgctgtga
    45361 ttgtcatgtg atacctctct tacttgaagt tgctgatata attgtttaaa agttgccata
    45421 gtcatcaaat cttgataata gtgagtagct agtctcctag gcagactgcc tgagaagtta
    45481 agacatcaaa cggcttagca atagtactca tgtggctcaa agagacttcg gcttcgtcca
    45541 catcactgta gggaccagct tccaaaattt ttgtctgcat gccttcatgt aaaacagcaa
    45601 tacaatttct cgtttcccct ttgattttga aaaattggtt agaaatgccc tgttcttgta
    45661 actgatgaat gataaagtca cccagcttac caccactaaa tcctgttgcc ataactgacc
    45721 agcttttgct aacattctgg tcacatttag ctccttatca ccagctgttt ttgccgttct
    45781 gtcaacacaa ttaaccgtgt ccatagccag acaatctaaa ggataggaaa tagcgacaga
    45841 aggatttaag ataactgtta aactcatctc ttcttcctaa tcgtgatact caccacgatc
    45901 ccatttttcc aaaaattctg taaaaaaatc aggatcttct tggttggcat gatgactttc
    45961 caagtgggct attttagcaa ttagcttctt attctcttca gtttctttgt attctgcctt
    46021 gataaaagca tctataatat cacacataag aagttcaccc gtgattttac caccaaagcc
    46081 aatcacattg gcattcaatt cttccttggc ataaagcgct gttgtcatat ctcgaacaag
    46141 ggctgaacga atacctggta ccttgttcac ggcattatta atgccaactc cagttccaca
    46201 aatacaaaca cccaagtctg cttgaccatt aaccacagct tcgccgactt tttttccaaa
    46261 aatagggtaa tgggttcttg tatggtcata agtgccacag tcaataacgt catagccttt
    46321 agatttcaaa aaatcagaaa cagccatttt ttcgttggtg acaatatggt cacaaccaat
    46381 cgcaattttc atcactttat cctcctttag gccattttat tgagcatgtc aacacgaatt
    46441 tgatgacgac caccatcgta gggaccagtc acaaaacctt tgacgatatt cttcgctaat
    46501 tctggaccta caatctctgc ccctattgtg atcatgcggg cattgttgtg accgcgcgtc
    46561 atgtaagcag agcgttcatc tgacacttca gcagcaacca ttccttttaa ttttgtggct
    46621 accataaagg gacctgctcc gtaagcatcc accataatgc ctaaacgacc ttctgcttca
    46681 ttaacggctt tcgccaccgc taatgtgtta tcaataaaat cactgttaat atcagtcacg
    46741 tcaataatgt catagccttc ttcttgaaga aaacttttga tcaattcttt taatgcattt
    46801 ccgtgtgcat ctgcacctaa aataatagcc atagcttttc cttttctaac ccaaaacgct
    46861 tgccaatcta gagaaggggg acatgattac ctgtccttag atcaagatga ttgccagtag
    46921 tcttagtgtc ttccattatt tctttcaaga caaagcactt gtgggttccc acagagcgcc
    46981 ttgccttaca tgagaaatac tcttttcacg ttagggagta aggtagaaat accttactcc
    47041 ctaatttttt agtcagtcac ctgacctgat tttatttcat tgttttcgca tactcagcgt
    47101 tacgtgcctt catttggtta gcataccaag caaagagacc taggtaaatg gccacaaaga
    47161 tgattagcgt gatgattgag gcaatctgac ctgttgtcgc ttgaccaatc acataaccaa
    47221 aaattttttc cattggtcct tcaagtgttg aatgcgtaat caaggaacca gcgcttgtac
    47281 ctgctggaaa agcccctact tttttagcca actctgtcgc aaatggagca atcatagttc
    47341 ctgacaagag gaataatggc aataagaggg aaccaaaaat aatcatgcga atcaaacgac
    47401 cacgtgtgac cacaagaaga gctggtgtca tccccatagc aataattcct gccagtggta
    47461 aaatcccatt gccaactttt gataggagaa cggcttcaat caacatgatt ggggcaagaa
    47521 tattggcaca ggcccagatt tcagcacgac cagcaatgaa gggccagtcc aaaccgatat
    47581 tgaaacgacg cccatgcatt ctagcattgg caaaattggt aatcccttgt gacaaaggct
    47641 caacagctgc gataaaccaa gagccaatca aagagaaaag ttccaagcag gcacccgcgg
    47701 taaagcctag gccaaaccaa tttttgatag attcaacagt gaaatgagga tctcccataa
    47761 tcccaatcac aaaacctaga gccattccga taaagaactt agagccccag aagccaattt
    47821 ttttgttcaa tttagcagca tcaaagtcat atttatccag ccccggaaag accttgtcaa
    47881 aaatcttatc taagaccata atgattggat tcatcatgta gttcatgtga gttgaagtca
    47941 ttggtgattg aggccctgtc ccaagaaggt catcaaaggt cggtttcatc aaatcagagt
    48001 taatgatttt cagtacccca acaagaataa ctgccacagt agcaatcaaa agagacacaa
    48061 agactggtaa atggttacga gccgcatacc acataatcaa tagacccgta atggacaaat
    48121 gccaaatatc aaaaatatcg acgtcaaggg tgtccgtttt tttcatgaca atcatgacaa
    48181 tattaaccac cagcatcacc agcaagaaat aaagggtgta aggggaaccc caggtaattg
    48241 ttgcaagcgg tgcccaaccg acatcaataa tgtttaaact gatccctgta tttttaacaa
    48301 aggcctgtaa agactctgaa aaagcccccg tcaaaattcc aataatggct ccaatccctg
    48361 tcaaagcgat ggctaactta atcccacctt ccagtgcctt tgtgaattta accccgaaaa
    48421 tcatggctaa acctgtcatg acaatcagca tgaccacacc tgatcccaac tgaataggaa
    48481 atcccagaat cttgttaata atgtctaaaa aaggttgcat catcttgtct ccttatgttt
    48541 ttattaactc actgcaaatg attttcttta atcacgcgct ctaattcagc aaaaacaggc
    48601 tcactcatga ttgggatacg atagagaatc ggtcctgctt ctactatggg aatcggaatc
    48661 tcaaatccca agtcagtttt tgcaatcggc gtaaaaatat cataacgaga caccaattct
    48721 tctgtgatgt ctttgaccat caccgcatca cagtgaacat cgtaaccacg gtcactcatc
    48781 tgagtttcaa tcgcatcttt aatttgatgg cttgagttaa cccctgcacc gcaggctgct
    48841 agaattttaa tcatgctgaa acactccttt ttctattaaa tagtttgaaa tagcttgttt
    48901 atcatctttt agactggcta gatagctaag attgcctttc gtaataaaga aggtaatgag
    48961 attggataga atcgtcgttt gaccctcgtt ttgactattg ataataaaga agaaatccgt
    49021 taccaaacag tcttcctctg gattaatcat gtgcttaaag gtaagggctt gactatttct
    49081 aacatagacc acttgatcaa ccaagcaata cttggtttct gtatgcggaa tagccgcata
    49141 caaaatatct gacccatctt tgaggtccac tttgagtcct gtcggaaata ccgcctcacg
    49201 ctccactaaa gcctgatggt actcttgcgt cacataccca accttttcca agtggctagc
    49261 aacaagatca aaaagctctg gctgcgtcct tgcttcagta aatagaatat taggaaacac
    49321 tccctaccgc ctttctataa gtatcaattt ttaacaactg tcatctcatc atcaacaaat
    49381 tggtaaccgt ttacaaaatc attatagtat aaccttcaac ataattcaac atttttacgc
    49441 aatatttttt gttctatatg tttgtttttg ttttgtttat tttgttttat tgaaatatga
    49501 aggtctatat gctatgataa acataagtaa ataaactata gaaaggagcc ccttagatgt
    49561 taaaaagaga aagacttcta aaaattaccg aaatagtcaa tgagcaaggt attgttactg
    49621 tgaatgatat tattcagact ttaaacgttt ctgatatgac agttcgtaga gacttagatg
    49681 aactggaaaa agccggcaaa ttgatccgca tccatggagg tgcacaaagc attaccatgc
    49741 ctaataaaaa agagcggtca aatattgaaa aacaaaccgt tcaaacaaag gaaaaatggg
    49801 aactagcttc atatgcgaca caactagtca atgatggtga aacaatcttt attggtccag
    49861 gaacaacttt agaatgtttc gctgagcagt taaaaaatcg tcagattcgc attgtgacca
    49921 atagcctccc tgtctttaac atccttcagg acagcgaaac cattgacctg attcttatcg
    49981 gtggtgaata ccgatctatt acaggagctt ttgtagggtc ccttgccagt caaaatatct
    50041 catccttaaa atttgccaag gcttttatta gctgtaatgg tatttataaa aacgatattg
    50101 caacttatag cgaaactgaa ggagagattc aaaagctagc ttttaataat tctattgaaa
    50161 aatacctctt ggtagataac caaaaattca atgcctacga cttcttcatt ttttaccatt
    50221 taaacaacat tgatgctgtt gttacagatt cgcaaataac tgaggacgtt atcgaacgtt
    50281 atagtcagtt cacgcaatta ttagtagccc acgaaaacaa ggagtaaccg tttcatcaag
    50341 aatgggcagc ctgtcagtgc atcgtcctta aacaccttct ggcaaaagtg atttgccccc
    50401 agagaggtgt ttttgttttg ctcggaacac acctgctaac gtcagagtct ttatgagtga
    50461 ccacaaaaaa agaagcccag attaaaaaac tggaacctct tttgagatga agtaaggcta
    50521 ccaaaataac ctaagaccat ttatataatt caaatttggt atccttcaaa gcacgcttga
    50581 ttaggaacgt taaaggacgt cctgtcacct tagcctctcc tttttccaaa tttacctgga
    50641 ccgattgcac accggggaca gctgataatt tttcagtgac cgttcttgcg caaccgtcac
    50701 atgtcattcc tgtaacctga taatgttttt ccattttgta ttctccttta aatggttttt
    50761 ccttttaatc gcagggcatt caaaacaact gatactgaac taaaactcat ggcaaaacct
    50821 gctaacatcg ggttcaatag tggacctcca aaaagatgaa gtaagcccat ggccactgga
    50881 atcatgagga cattgtaaat gaatgcccaa aataaatttt ccttgacaat tctcatagtc
    50941 actcgactca atgacatagc tttcaccaaa tccagcatat caggtttcat cagaatcaca
    51001 tccgccgatt caatggcaat atctgtccct gaccccatag ctatcccaat gtctgctaca
    51061 gccaatgccg gagcatcatt aataccatca cctaccattg caacttttct cccttgagag
    51121 cggaggtctg ctatcacgcc tgctttttga tcaggtaaga cttgactgat cacatctgta
    51181 atccccactt gagaggcaat agccttagcg gtagcatcgt ggtcacctgt tagcatgatg
    51241 gtgtgaattc ccagttgatg caaagcttcg acggtagctt gactatcttc tttgacagcg
    51301 tcagcaatac tgaaaagagc tcgtagctgc ccctctttag cataatatac aagggtttgg
    51361 cctttctcag aagccttagc acaaaccgac tgaacacaag ataaatcgat accaagaatt
    51421 cccattaaag cagcatttcc aatcgccaaa cgacgccctt caagctgacc agtaactcct
    51481 aggccggtta aagactcaaa ctcttccact ggtagactat cgtaaccagc ttcctgagca
    51541 gcacgaacaa tagcctcccc taaggggtgt tgagaatagg cctccaagga agcagcttct
    51601 tgcaagataa ggtgatcatc tcccgaaaaa gttaggagct tttgaagact tggtttcccc
    51661 tccgtaatcg tccccgtttt atcaaaaaca accgtatcta cctgatgtgc ttgttctaag
    51721 atgtcaccac ctttaaaaag aataccattt tcagctgcac gccctgttcc aaccataatc
    51781 gctgtcggcg ttgctaaacc aagcgcacaa gggcaagcaa tgactagaac agcgatggct
    51841 gttgtcaaac taaaacgaag actttcctgc atgaatacca accaaaacac ccctgttaac
    51901 agagctaata ccaaaaccac cggaacaaag acacctgaga ctctatcagc aatagctgca
    51961 ataggtgctt tattttcttg agcattctca actagttgga taatttgtgc taacaaggta
    52021 tcatttccaa gcttatctgg tttaatcgtg agggcgcctg gcccattcaa gctgcccgca
    52081 aagacattat ctttttctcc cttttcaaca ggcaaacttt ccccggttaa aaaggattca
    52141 tccaaactgc tatgtccttc cacgataata ccgtcaatag aaactttttc acctggttga
    52201 accaggacaa ggtcatcata gctaacttct tctaatggta acaattgcca ttggctgttt
    52261 ctccataccc tcacaacagt agccttcaag gataggagct tttgaatagc ttgagaagta
    52321 cggcccttag aacgactttc aaaatacttt cctagaccga tgagtgtcaa aatcactacc
    52381 accgattcaa aataaaggga gtgcacaaag tgcgcccggc ccagactgat ttcaaccact
    52441 ccataaagac tataagcaaa agcaaagctg gttgctaaag ctaccaagga gtccatatta
    52501 ggatgtccct taaacaaagc tttaaaccca ttatggtaat aatgacgatt taatactaag
    52561 actggaatcg tcaagagtag ctgactaaag ctgtaaacga aaggatagcg atctggttgc
    52621 agaaatggag gaagccataa gcccaccatg tgacccatag ctaggtataa tataggaatg
    52681 gtgaataggg cagaccacaa gacctgagac caaataacct ttaggtcttc gtcacgacga
    52741 cctgtttgca tgtgttttgc atgttcatca aagacttgtg caccataacc agcagctttc
    52801 accgcttgaa taactttggc cgtttctaaa tcgtcatctt gataagtaac cgtgagcttc
    52861 tcagtcgcta aattcacaac agcctgttct acttcaggca attgattgac cgttttttca
    52921 attgttagag cacaagaggc acaactcatt ccctccacta aaaatatctc tttagccatt
    52981 aatacgtctc ctttctctga cagtggcact gcccaggcag acaattacac ggcacctcaa
    53041 gaactgcatt ttcttttttg acttctagta gctcttggaa tgccccgatt tcctctaagg
    53101 tcatcggtaa gtgataaagc cgttcaagaa gtagacgcgt gtgttgccgc tgacaaatac
    53161 cgtctaaaac agtcgctagt gcttcttttt gtagcaaagt ctcatccaaa agagcttgat
    53221 aaatataagc tcttccttgc cgataagacg ttaaaaaatt ttttttaact agcctaccaa
    53281 tgagggtctt aatagtggaa tctgaccatt gatatttttt ccttaaaata gtaataatat
    53341 cgctactttt aatatcacca ctggcccaca ccacacgcat gacttcccat tcagcagctg
    53401 atatctgttg cataacgtca ccttcattca caatatattt acgtttgtaa attaattata
    53461 acgaatttaa ctacatttgt aaataaataa cttttctcat ttttctatcc ttatattgac
    53521 aaatgaatcg tttttactat aatattattg caataaaaat aacatttaca ttaaggagat
    53581 acccttgaaa catcctattc gaaaaacact tgtcacctta ggtctattac tgaccttatg
    53641 cctgccgact ccagttgcag ctagttctcg ttcttggaag agctggttta tcgagcaata
    53701 tttctggtta aaacgtgaca agagttacta ttctaaacaa gatgacccaa gcttccaaag
    53761 ataccttgat gcctgtcgtg aacaatctga taaaccttat caactagata ctaatttagt
    53821 caatggtccc cttgttcaag aaaatcttta tggcatgcag gtttactctt ggaatgataa
    53881 tggaaaacct gatcaaaaaa caattattta tcttgccggt ggttcttatc ttaacaatcc
    53941 aacgacatac catattaata tgttaaagac attatccaca agtcttgacg ctaaaattgt
    54001 tttacccatt tatcctaaag ccccacgtta tacctataac tatactatgc caaaattggt
    54061 caatctttac caacactatt accataaaaa tcagaatgtt ttccttatgg gagattcagc
    54121 aggtggaggt ttagccttag gtctagctca tgccctacac aatgaatcag tccctcaacc
    54181 aaagcagctc gttcttttat caccttggtt agatgttact atgtcacacc cagagattcc
    54241 agaatacgaa gatgctgatc ctattctatc ttcttgggga ttaaaacgag tgggagaatt
    54301 gtgggcctac tctgctgata ataccaacca tatttatgtc agtcctaaaa atggtccgat
    54361 cacttatcta ccaccaatta ctttatttac tgggacacga gagatctttt atcctgatat
    54421 tcgcgattat gccgcaaaac tgaaagccgc taaccacaat attaccttta ttacccaaga
    54481 aggcatgaac cacgtttacc caatttatcc tatcgaagag gcaaaaacag ctcaatacca
    54541 aatcattgat gccatcaaca aaactcctta atgaggggaa taactcctac ctgccaccag
    54601 ttaatctggt gttttttttg tagtttgcac cacaaaataa caattaaaaa gagccctatc
    54661 ataatgataa ggctctttct gtcataacat ccgcaccgca atacttagga tttgttgtca
    54721 agatctcgta agagttgatc aattttatta ccataggcga tggaattgtc tttttcaaac
    54781 accaaatctg gaattttgta catggtcaat tgtttgccaa gttcacgttt aatcgttcca
    54841 gtcgcttttt caagccctgt ttgtgctttt tgattatcag atgccaagtc actcataata
    54901 gtgtaataaa ctttagcaag tgacaagtca ccctgcattt gcacttctgt aatcgttact
    54961 ccctgaacgc gtggatcacg cacttttttc tgaagaatat catttacttc gcgcttgatt
    55021 tccatcccaa cgcggtcaat acgatgatta gccatagcca tttcctttcc acattttaaa
    55081 acaaaagcta ggctgaaagc ccagcttatg acattgctac ctgttttgat aacgacaatt
    55141 aaagggaatc tgagaattcc atgacgagac tggaaggaat gccccaagta gctcatctgg
    55201 tagaaaaagt cattatttcc gacagaggaa aaacgttaca tctcagacct tcttagcccc
    55261 tattttctca caatttcttc cataatatag gcttcaatag tatcgtcaac tttaagatcg
    55321 ttaaagtttt caatcatcaa cccaccttct tgagcattac cgacttcttt aacgtcatct
    55381 ttgtaatgtt tcaagctggc cagtttacca tcaaaaataa cgacactatc acggataaca
    55441 cgaacactcg aatcacgtgt caccttaccg ttgattacca taaagccacc aattgttccc
    55501 actttagaaa ctttgaaggt ttcacggata atggcttcac caagaatttt ttcttggtaa
    55561 acagggtcaa gtttcccttt catggcttct tctacttctt caatgacttt atagataata
    55621 ctatgaagac gaatttccac atcatccgta tctgcttgtt gacgagcttg tggggtaggg
    55681 cgaacgttga aaccgataat aacagcattt gatgcttcag caagggtcac atcagactca
    55741 ttgatagcac ctacagcaga gtgaacgaca ttgacacgga caccttccac ttcaatcttt
    55801 acaagagatg ctgcaagggc ttcaacagaa ccttgaacgt ctgctttgat gatgacatta
    55861 acagtcttga tttctcctgc tttaagggta tcaaagagat tatcaaggct aacacgctgt
    55921 gtattttgac gttgtttgag gagggcacgt tttgatcgct cctcaccagc tgcacgcgca
    55981 gctttttcat cagcgtaaac agcaaagtga tcaccagcca ttggtgtttc gttcaaacca
    56041 gtaatggaaa ctggtgttga tggctccgca gatttaacac gacgcccaag gtcatttacc
    56101 atggcacgga cacgaccaaa ggtattacca acaacgattg gatcttgaac atgaagagtt
    56161 ccttgttgga caagaagcgt agcgatagca cctttacctt tatctaagcg tgcttcaatt
    56221 acagtaccaa tagcacgaac cgtcggatca gctttcaatt cttccacttc agcaaccaaa
    56281 aggactgttt ctaatagttc atcaatattt ttattaaatt tagcagatat ctctacaaat
    56341 tcgcactctc cgccccaagc agttgagatg ataccatatt ctgccaattc tgcgataaca
    56401 cgttctggat tagcacctgg tttatcaatt ttgttgatag caacaataat tggaacacca
    56461 gcagcttttg agtgattgat cgcttcaatc gtttgtggca taacaccatc atcagctgca
    56521 acaatcaaaa tggtgatatc ggtaacggaa gctccacgtg cgcgcatact tgtaaaggcc
    56581 gcgtgtccgg gagtatccaa gaaggtaatt ttcttgccag cttcttcaat ctggtaagca
    56641 ccaatatgct gagtaattcc tccagcttct cctgttgcca cacgtgagtt acgcaaagta
    56701 tcaagcaaag ttgtcttacc gtggtctacg tgtcccatga tagttacaac aggtgcgcgt
    56761 tctacaatat tttcaggatt gaggtaattt tcatcctcaa agaagcgctc gatatcagca
    56821 tcatctactt ctaccttagc cttagcctca ataccataat ctaccatcaa taattcgata
    56881 gtgtcaccat ctaaagattg gttttgagtt gccataacac ccatcataaa cagcttttta
    56941 acaatttcag cgggttcgcg tttgatacgt tttgcgattt cggcaactgt catgccttca
    57001 gtatattcaa attcttttgg caattcatgg aatttacgct cggttactgg ttttggagcg
    57061 gtgttggtat tcttaacatt tttgcctttt ttggtctttt tatttttatt ccagttacta
    57121 tttttttgat ttctcacttg gttttgacta ttccaactct tcttattttt cttttgtcct
    57181 tcttgtgaat gatgagttaa ctcacgattc ttttcaggac gtgcttgttt tttacggcga
    57241 ttatcagcag tagatttagc actaatattt gatgacttta atttagtttc tgcttcttca
    57301 atagatttca atgctgcttc acgatggctc atctcctcag cttgtgcttt tagagccgct
    57361 tctttagctt cttgtcttgc caactcagct aaacgcttag cttcttgctg tgcacgaaaa
    57421 cgtgtttcac tttggcgtga gtattcagca ttttgttcag cttttaaggc agcagcacga
    57481 gctttgaaat caattttagg acttttcgat tgaggctctt gtcgtccttg ctttttttgg
    57541 tttgtgaatc gaccattctt gtgattctcc ttagcaaagg aacggttacg atcaccaact
    57601 agctgatgat tatcatgtct aggtgataca ttcgatgctt ttcgctcaat acgcttatcg
    57661 tttgtatcac cttgagaacg atgggtaatc ctcttatctc cttgttcgtt agtttgatga
    57721 ttattgggct ggcgataacc tcctcgacgt tcttgagaac gatgagaaga gccatttgct
    57781 tgttttcgag cagcctgttc ctttgcacga gcctctcttt cagctttaaa attacgactc
    57841 tttggcttag agacctgtgt tttctctaca tgctcagaac ctgtttcttt gaccactgtt
    57901 acagagggtt gagcaacttc tttgggtttg actgtttgag aagctgtcac atcaggttta
    57961 gaagcctttg aaaaacttga aataatcttt ttcgcatccg cttcttcaac acttgatgca
    58021 tggcttttca catctaatcc taagtattta gcgtgttcaa ccacctcttt actactttta
    58081 ccaatttcct tggcaatttc atgtaatctt ttctttgaca atttgtgtcc tcctattcat
    58141 ctattccata agagtcctca ttttctttga aaatccagca tctgcaatgg cagcaacctt
    58201 ccttggttta cctagtgcag cacttaattc cagtgcattc aacactgtgg agacttctac
    58261 attataataa ttacttttat ctgtcacttt ttttgtcaca ttaggaccag catcattggc
    58321 taaaaatact aatattacct gctggtgttg aatagctttt actactagtt cttcacctga
    58381 aatcactttt ccagcccgtt gagctagacc aataagacta gataatcttt ctaaattagt
    58441 caagacctaa ctctcttctt ttgattttgt gatcaacata agcaatcaag tcatcgtaaa
    58501 aactttctgg aatgtccatt gagaaactcc tgttgaaaac ttgttttttc ttggccatta
    58561 aggcctcttg attgtctaat ttgatataag caccacgacc attttgttta ccagttggat
    58621 caatgaaaac ttgaccatcc ttagttttta caatcctaag aagatctcgt ttagcaataa
    58681 tttcaccaga aacaagtgat tttcgtaaag gtattttttt gactttagac atcaaacacc
    58741 tcttttctta tacttcctcg gcagtcataa catcgtcaac aactggctct tccacagctg
    58801 ttgctgcttc tttttccgcc tcaagacgat catattcgct agctgatttg atatcaatac
    58861 ggtagccagt caaatgtgcc gctaaacgaa cattttggcc gcgacgcccg atagctaatg
    58921 ataatttgct atcaggaact actaccgtag cacgtttaag atcttcatcg tcaaaaagta
    58981 ccatgtctac ttcagccgga gcaatggcat tgtagatgaa ctcagctgga tcatctaccc
    59041 attgaatcac gtcaatattt tcttcaactg gaatttcaag ccccgtctta gcatctacac
    59101 gttttggatg gaacttgctg atgacctttt taatattact tccaccacgt ccaacaattg
    59161 ttccgatggc atcaacgtta ggattatgac tgcgcacagc aaccttggtg cggtcacctg
    59221 cttcacgaga aacactcatg atttcaacag taccatcaaa gacttcagga atttcttgct
    59281 ccataatacg tttgatgaat tctggatggc tacgactaac aaagacgtta actcctttag
    59341 gattattttc aactttgtag acataaacgt caatacggtc atgtgatttg aaagtttcac
    59401 ctggaatctg gtcttggtga gacaattgtg cttctaagga accaagattg acataaataa
    59461 aacgttgatc aaaccgttca actgttcctg tcataatttc accttcatgc tctttgtatt
    59521 cattgaacat cacttcacgc atttggcggc gcatcttttc cataatagtc tgtttagctg
    59581 attgagctgc gacacgtcca aattcattga cagattcttc aaagcgaatc ttatcaccca
    59641 attcataggc agaactgata gcaagagcat ctttcaaact aatctcaagc cggctgtcaa
    59701 aaacttcttc aacaacctca cgaaccgtaa atacttgaaa atcagctgtt ttttcattaa
    59761 attcaataac acatgattct gattgaccat agcgacgttt ataggcagac tttaatgatt
    59821 ctgtcactgc gtcaataata tctgctttat caatgtgttt ttcttcttcc aaaatacgga
    59881 aggcttctag catttctttg ctcataatgt tatctacctt gcttgtaaag cttggcaaaa
    59941 atcctttctt aaattaatac gatcaatagc cccctaaagg ctcccttaat tccttttgtt
    60001 tagcttgtat aaactctata ggtgttgttt caaatttgct taaatgttcc taaaaaagtt
    60061 tagtgctttg aaataaaagt tataatttga cagccattct caccttagca actgcttgat
    60121 atggtatatt cacgatctta tgacgtgttt tatcaagata atcaatggtc aacgtttcac
    60181 catcaaaggc taataagtct ccttgaaaaa ctttcacctt atcaatagct tgataaagac
    60241 tcacattgat ataagagcca actgctgctt ttaaactgtc agctgtcttg agcggacgct
    60301 ccaaacctgg actagagact tctagcatat attggtttgg aaaaggatct ggatcaatcg
    60361 tatctaacag cggactaata atattcgtca agtcagatgt atcttccact gtgattccac
    60421 cttccttatc gactaaaata ctaaggatgt agtcactacc cattttgtca tactcaacgt
    60481 ctactaattc ataaggtgcc ttaatctcag gcgttactgt cttggttacg atatcgataa
    60541 tagatgtatt tgctattttg attacctcct ctatttttat gctcttctct aagataattg
    60601 gtccttggga atccacttaa tacaaaagtg gcgagacata tcccaccacc tttctgtatt
    60661 atttaagcct attataccat aatttaagag gaatgcaagg atatgcactt tgttttactc
    60721 acaaggagag gaggggattg ctggtagcta tctcaccgtt ttcatccttt gcaagacact
    60781 taaacgttgg tatttttaaa atatagaaag gatagaaaaa gatgaaaaaa taaaaataaa
    60841 cgggtgattg acgggtaatg ttactccaat tccatcaaca cttcttccac tttttcatct
    60901 gtcacaactt ctccattctt tactttctca tgtggcaaca cataatcaaa gatctgtccg
    60961 tttttgcgca caatcgctac tgtgtctcct gtgatatacc catggtcgat tgcttgctta
    61021 aattcgtcgt atgttagcat agattacctc cttatcttat tattcgtatt ttacaggaaa
    61081 taatataatt aaatttgaag cttatttaaa a
//
LOCUS       DAXWIQ010000005        59468 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000005 DAXWIQ010000000
VERSION     DAXWIQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 59468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59468
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.005"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..371
                     /locus_tag="ACGEUI_000277"
     CDS             <1..371
                     /locus_tag="ACGEUI_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HFF8165145.1"
                     /translation="LLKTIGINVSLRLLILNSLIYIIFAYVTYLINHIISYIFSTGVA
                     LGISMVGVIAAAFCETSLGDKVWFLIPWAWLLRISDTLYNQQKMAIVPLIVIFFVSCT
                     VALLHIRIFKRWNQDCLTSS"
     gene            563..781
                     /locus_tag="ACGEUI_000278"
     CDS             563..781
                     /locus_tag="ACGEUI_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8165146.1"
                     /translation="MYPRIRNLREDNDFTQKFVANLLSFSHANYAKIERGEVALMADV
                     LVQFYKLYNVSIDYLLGQTDYPYRFHNK"
     gene            complement(852..995)
                     /locus_tag="ACGEUI_000279"
     CDS             complement(852..995)
                     /locus_tag="ACGEUI_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165147.1"
                     /translation="MGISPTGLLRWQPERSEVSLSLFVVMADREPLYFIANNYRSYHG
                     VSH"
     gene            1082..1315
                     /locus_tag="ACGEUI_000280"
                     /pseudo
     CDS             1082..1315
                     /locus_tag="ACGEUI_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828144.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
     gene            1272..2459
                     /locus_tag="ACGEUI_000281"
                     /pseudo
     CDS             1272..2459
                     /locus_tag="ACGEUI_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943304.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            2617..3852
                     /gene="pbp3"
                     /locus_tag="ACGEUI_000282"
     CDS             2617..3852
                     /gene="pbp3"
                     /locus_tag="ACGEUI_000282"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase PBP3"
                     /protein_id="HFF8165148.1"
                     /translation="MIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRV
                     LYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVP
                     FDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSELHFVNQMREQLSHWGITSGKILN
                     ASGLPNEVLKDHRYPGSALEEENMLSAQDVAIVTMHLLEDFPEILEITKQTEVDFAGN
                     SIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRLITVILNADGSDKN
                     QNTRFEQANRLLDYVARTYCRRKILKKGSLVSERSLPIQDGQVKELPISVAEDVTIIL
                     QQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQS
                     LKKASDLQLWWRDFLEKRR"
     gene            complement(3969..4931)
                     /locus_tag="ACGEUI_000283"
     CDS             complement(3969..4931)
                     /locus_tag="ACGEUI_000283"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFF8165149.1"
                     /translation="MNNRHKRRQKRKAFTLINLILLFACILGLTLIFYLYQGSNKFGL
                     SDVSRFVPKISLFHHKKTAKKETTKLKKTHFDSSKSQKKAHSKLTWTKQETPVKIPIL
                     MYHAIHVMSPEETANANLIVNPDLFDQQLQKMKDEGYYFLSPEEVYRALSNNELPAKK
                     VVWLTFDDSMIDFYNVAYPILKKYDAKATNNVITGLTEMGSAANLTLKQMKEMKQVGM
                     SFQDHTVNHPDLEQASPDVQTTEMKDSKDYLDKQLNQNTIAIAYPSGRYNDTTLQIAA
                     RLNYKLGVTTNEGIASAANGLLSLNRIRILPNMSPENLLQTMEP"
     gene            5263..6540
                     /locus_tag="ACGEUI_000284"
     CDS             5263..6540
                     /locus_tag="ACGEUI_000284"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFF8165150.1"
                     /translation="MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHI
                     VGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRI
                     RPLTERLVQETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAM
                     VVVCPSIGLDHQAILGETYANIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGA
                     SIWEWQEQFQMAENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLQDRYP
                     RLTPDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYNDKKLHILF
                     GAIDTKPIADMLVALEQIGDLQVTSFHYPNAYPLEKYPERFGRVADFKDFLALRKHAK
                     ADDFFVITGSLYFISEIRQYWEKHIEKSVLLTH"
     gene            6587..7153
                     /gene="folE"
                     /locus_tag="ACGEUI_000285"
     CDS             6587..7153
                     /gene="folE"
                     /locus_tag="ACGEUI_000285"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFF8165151.1"
                     /translation="MSINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKMYAEMFL
                     GLGKDPKEEFTAVFKEQHEDVVIVKDISFYSICEHHLVPFYGKAHIAYLPSDGRVTGL
                     SKLARAVEVASKRPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRGIKKPGS
                     KTITTTARGLYKESRAERQEVISLMTKD"
     gene            7162..7962
                     /gene="folP"
                     /locus_tag="ACGEUI_000286"
     CDS             7162..7962
                     /gene="folP"
                     /locus_tag="ACGEUI_000286"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFF8165152.1"
                     /translation="MKIGKFVIEGNAAIMGILNVTPDSFSDGGSYTTVQKALDHVEQM
                     IADGAKIIDVGGESTRPGCQFVSATDEIDRVVPVIKAIKENYDILISIDTYKTETARA
                     ALEAGADILNDVWAGLYDGQMFALAAEYDAPIILMHNQDEEVYQEVTQDVCDFLGNRA
                     QAALDAGVPKNNIWVDPGFGFAKSVQQNTELLKGLDRVCQLGYPVLFGISRKRVVDAL
                     LGGNTKAKERDGATAALSAYALGKGCQIVRVHDVKANQDIVAVLSQLM"
     gene            7969..8328
                     /gene="folB"
                     /locus_tag="ACGEUI_000287"
     CDS             7969..8328
                     /gene="folB"
                     /locus_tag="ACGEUI_000287"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFF8165153.1"
                     /translation="MDKIVLEGCRFYGYHGAFKEEQTLGQIFLVDLELSVDLQAASLS
                     DQLTDTVHYGMVFDSVRQLVEGEKFILIERLAGAICEQLFNEFPPIEAIKVAIKKENP
                     PIAGHYKAVGIELERQR"
     gene            8325..8825
                     /gene="folK"
                     /locus_tag="ACGEUI_000288"
     CDS             8325..8825
                     /gene="folK"
                     /locus_tag="ACGEUI_000288"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFF8165154.1"
                     /translation="MTIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETT
                     AWGKTGQADFLNMACQLDTQLTAADFLKETQAIEQSLGRVRHEKWGSRTIDIDILLFG
                     EEVYDTKELKVPHPYMTERAFVLIPLLELQPDLKLPPNHKFLRDYLAALDQSDITLFS
                     AQQTEF"
     gene            8975..9862
                     /gene="murB"
                     /locus_tag="ACGEUI_000289"
     CDS             8975..9862
                     /gene="murB"
                     /locus_tag="ACGEUI_000289"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFF8165155.1"
                     /translation="MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIV
                     AYANKENMPWLVLGNASNLIVRDGGIRGFVIMFDKLNTVHLNGYTLEAEAGANLIETT
                     KIAKFHSLTGFEFACGIPGSIGGAVFMNAGAYGGEISHIFLSAKVLTPSGEIKTISAR
                     DMAFGYRHSAIQETGDIVISAKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSV
                     FKRPPGHFAGQLIMEANLKGHRIGGVEVSEKHTGFMINVADGTAKDYEDLIAYVIETV
                     ENHSGVRLEPEVRIIGENL"
     gene            9908..11062
                     /locus_tag="ACGEUI_000290"
     CDS             9908..11062
                     /locus_tag="ACGEUI_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8165156.1"
                     /translation="MTKPIITFNNVSKTFEDSGTQVLKNINFDLEEGKFYTLLGASGS
                     GKSTILNIMAGLLDASSGDIYLDGERINDLPINKRDIHTVFQNYALFPHMTVFENVAF
                     ALKLKKVDKKEIAKRVKETLKMVQLEGFENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVANFIGESNIINGTMIEDYLVSFNGKEFESVDGGMRPNEPVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIEGEVIG
                     LDFTPEDLHIMRLNETEEEFDARIEEYVEMDEPEDGLINAIEEERNEENL"
     gene            11046..11840
                     /locus_tag="ACGEUI_000291"
     CDS             11046..11840
                     /locus_tag="ACGEUI_000291"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8165157.1"
                     /translation="MKKTSSLFSIPYFLWILFFVVAPVTLLFYKSFFDIEGRVTLANY
                     ETFFSSWTYLRMSVNSILYAGIITLVTLLISYPTALFLTRLKHKQLWLMLIILPTWVN
                     LLLKAYAFMGIFGQQGGINSFLTFMGIGPQQILFTDFSFIFVASYIELPFMMLPIFNA
                     LDDIDHNVINASRDLGASEFQAFSKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLTTQNWGMGSTIGVVLILTMVAIMWLTKEKSK"
     gene            11837..12613
                     /locus_tag="ACGEUI_000292"
     CDS             11837..12613
                     /locus_tag="ACGEUI_000292"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8165158.1"
                     /translation="MKKFANLYLASVFVLLYIPIFYLIFYSFNKGGDMNGFTGFTLEH
                     YQTMFEDSRLMTILLQTFVLAFSSALLATIIGIFGAIFIHHVRGKYQNAMLSANNVLM
                     VSPDVMIGASFLILFTSLKFQLGMSSVLLSHIAFSIPIVVLMVLPRLKEMNQDMVNAA
                     YDLGANYFQMLKEVMLPYLTPGIIAGYFMAFTYSLDDFAVTFFLTGNGFTTLSVEIYS
                     RARQGISLDINALSTIVFFFSILLVIGYYYMSQDKEEKHA"
     gene            12606..13679
                     /locus_tag="ACGEUI_000293"
     CDS             12606..13679
                     /locus_tag="ACGEUI_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8165159.1"
                     /translation="MRKLYSFLAGVLGVIVILTSLSFILQKKSGSGSQSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMYTKIKQGGTTYDIAVPSDYTIDKMIKEN
                     LLNKLDKSKLVGMDNIGKEFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKAPMHWED
                     LWRPEYKNSIMLIDGAREMLGVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVA
                     DEMKGYMIQGDAAIGITFSGEASEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEK
                     EAYAFLNFINRPENAAQNAAYIGYATPNKKAKALLPDEIKNDPAFYPTDDIIKKLEVY
                     DNLGSRWLGIYNDLYLQFKMYRK"
     gene            complement(13734..14399)
                     /locus_tag="ACGEUI_000294"
     CDS             complement(13734..14399)
                     /locus_tag="ACGEUI_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8165160.1"
                     /translation="MNVLIIEDDPMVDFIHRNYLEKLNLFDRIISSDSMKAVQSILTD
                     YAIDLILLDIHITDGNGIQFLEKWRTQHIPCEVIIISAANDGNIIRDGFHLGIIDYLI
                     KPFTFERFQESIQQFVTHREHLANQQLEQAQIDQLKCLTSKKDTKNKQLLEKGLSEST
                     FQWIMENIKVFDQPFTIQELASACHLSHVSVRKYIAYLEENKQLNSQQIFTKVGRPYR
                     VYW"
     gene            complement(14380..15921)
                     /locus_tag="ACGEUI_000295"
     CDS             complement(14380..15921)
                     /locus_tag="ACGEUI_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HFF8165161.1"
                     /translation="MKKPLRLWASLSLILVSMIVVTTSLFYGIMLHDTHQSIKNQETH
                     LLTSTGKMLASHQAIKELLLNNQPNAKTTAYTNSIASIYNLDYVVVMNMKGIRLTHPN
                     PKNIGKPFQGGDEEAVLAGKKVISTAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTT
                     LNDVALTSKRNYTLSLLLCLLISLLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLD
                     QIEAAVFVVDKAGILQLCNQAGQKLIARKCQLGKPTGNSFNYLFPDFPKLSLQEGHEQ
                     LFRYGEEDYLLAISPICVKNDHRGHIIFMREAVKAIDTLDQLAYTTAYASALQAQTHK
                     FMNQLHVIYGLVDIAYYDQLKIYLDSILEPENEILTSLSVLVKEPLLASFLIGEQEKY
                     QELNVHLKIDVLSEIPHSATKNQLNNGLMIYRFIHTNLLTTLRPKSLVLSIQHDQNHL
                     ISHYTLTDNWIDLERVQPIFDLPYFQQLLTDTNSQFHQHQTVTELQFSVTTPYVGGQH
                     ERFNH"
     gene            16082..17413
                     /locus_tag="ACGEUI_000296"
     CDS             16082..17413
                     /locus_tag="ACGEUI_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFF8165162.1"
                     /translation="METISKKMPQKDLSEHSKAWQNRRIGSVPLPVYLVLATLILVTG
                     WLQQLPVNMLGGFAVILTLGWLLGTIGATIPGLKHFGGPAILSLLVPSILVFFNLLNP
                     NVLEATNVLMKQANFLYFYIACLVCGSILGMNRKILIQGLFRMIIPMLLGMVCAMGVG
                     TLVGVILGLDWQHTLFYVVTPVLAGGIGEGILPLSLGYSAITGVGSEQLVAQLIPATI
                     IGNFFAILCTALLNRFGEKHPSYSGQGQLVKIGHSEDMSDALKDNSGALDVKLMGAGV
                     LTACSLFIAGGLLQHLTDFPGPVLMIILAAFLKYLNVIPQETQNGAKQLYKFISSNFT
                     FPLMAGLGLLYIPLKEVVATLSWQYFIVVISVVLTVVSVGFFVSRFLNMSPVEAAIIS
                     ACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRIIFS"
     gene            17444..18610
                     /locus_tag="ACGEUI_000297"
     CDS             17444..18610
                     /locus_tag="ACGEUI_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HFF8165163.1"
                     /translation="MKNQLGQLALEQAKTFGGKLEVQPKVDIKTKHDLSIAYTPGVAS
                     VSSAIAKDKTLAYDLTTKKNTVAVISDGTAVLGLGDIGPEAAMPVMEGKAALFKAFAG
                     VDAIPIVLDTKDTEEIISIVKALAPTFGGINLEDISAPRCFEIEQRLIKECHIPVFHD
                     DQHGTAIVVLAAIFNSLKLLKKSLDEVSIVVNGGGSAGLSITRKLLAAGATKVTVVDK
                     FGIINEQEAAQLAPHHLDIAKVTNREFKSGTLEDALEGADIFIGVSAPGVLKAEWISK
                     MAARPVIFAMANPIPEIYPDEALEAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAAKGIASLVPDDALSTTNIIPDAFKEGVAEIVAKSVRSVVLKS"
     gene            complement(18693..19742)
                     /locus_tag="ACGEUI_000298"
     CDS             complement(18693..19742)
                     /locus_tag="ACGEUI_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8165164.1"
                     /translation="MKAATYLSTGNLQLIDKPKPVIIKPTDAIVQLVKTTICGTDLHI
                     LGGDVPACKEGTILGHEGIGIVKEVGDAVTNFKIGDKVIISCVTSCHTCYYCKRGLSS
                     HCQDGGWILGHLINGTQAEYVHIPHADGSLYHAPDTIDDEALVMLSDILPTSYEIGVL
                     PSHVKPGDNVCIVGAGPVGLAALLTVQFFSPANIIMVDLSQNRLEAAKTFGATHTICS
                     GSSEEVKAIIDDITNGRGVDISMECVGYPATFDICQKIISVGGHIANVGVHGKPVDFN
                     LDELWIKNITLNTGLVNANTTEMLLNVLKTGKIDATRLITHHFKLSEVEKAYETFKHA
                     GANNALKVIIDNDIS"
     gene            19978..20709
                     /gene="aphA"
                     /locus_tag="ACGEUI_000299"
     CDS             19978..20709
                     /gene="aphA"
                     /locus_tag="ACGEUI_000299"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFF8165165.1"
                     /translation="MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQAT
                     VKLISIADIASSLEGQKPITVSFDIDDTLLFTSQYFQYGKEYITPGSFDFLHKQKFWD
                     LVAKRGDQDSIPKEYAKQLIAMHQKRGDKIVFITGRTRGSMYKKGEIDKTAKSLAKDF
                     KLDKPIAINYTGDKAVKPYQYDKTYYIKKNGSQIHYGDSDEDINAAKEAGARPIRILR
                     APNSTNLPLPKAGGYGEEVLENSAY"
     gene            20887..22419
                     /locus_tag="ACGEUI_000300"
     CDS             20887..22419
                     /locus_tag="ACGEUI_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFF8165166.1"
                     /translation="MENHKNEFTFSNKSIIAYVWRGVVVGIIAGVIVSLFRLLIEVTA
                     DWVIEWYRYAHINSLLLLPILSVSLLAVLFVGFLVKSDSDIKGSGIPHVEGELKGLMS
                     PDWWSVLWKKFLGGIMAISMGFMLGREGPSIQLGAMSAKGLAKFLKSSRLEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLIWITALVASLVANFISLNIFGLKPVLA
                     MSEAMPFLGLNQYWLLLLLGLFLGCLGYLYEIVILNFNKLYVILGSWLHLPDYFYGII
                     MVFLILPIGYYLPQLLGGGHGLILSLSNQQLPLMTIFFYFIIRFIVSMFSYGSGLPGG
                     IFLPILTLGALAGLLFGQIASQLGLLNQSFLSLFLILGMAGYFAAISKAPLTGMILVT
                     EMVGDLKPLMAIAVVTFVSYLVMDLLNGQPIYEAMLDKMAMKHPTNLVEPTLIELTVG
                     DKIAGKYVKELKLPENVLITTQIHHQKSQVVSGNTRLLSGATIFLVVNEADTGFVREV
                     LM"
     gene            22582..23196
                     /locus_tag="ACGEUI_000301"
     CDS             22582..23196
                     /locus_tag="ACGEUI_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFF8165167.1"
                     /translation="MLEIVSEELRHYQEQKLIEYRNKNQLAPKGGIVFAGDSLIEFFP
                     LKKAFGSCLPIINRGIAGIDSQWLLRHFSVQITDLEPKHIFLLIGCNDIGLGYDKCHI
                     VKTIVELISQIRSHCVYSQIYLLSLLPVSNNPRYQKTVKIRTNAMIDAINKDLAMIPT
                     VEFINLNTCLKDEKGGLSDENTLDGLHLNFPAYAKLAEIIKSYI"
     gene            23376..24503
                     /locus_tag="ACGEUI_000302"
     CDS             23376..24503
                     /locus_tag="ACGEUI_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8165168.1"
                     /translation="MKFEKKQVFYLVLTFILCYGILANWRNGTAIVTTIYKTSLPFFY
                     GAAGAYIVNIVMSAYEKVYVYIFKDWSHVLKVKRGICLLLAYLTFFILITWIISIVIP
                     DLITSISTLTKFDTITIQEVVNNLEHNKLLARTIQYIGGDGKLTETIANYSQQLLKQF
                     LTVLTNILTSVTVIASAIINLFISFVFSLYVLASKEDLCRQGNTLVDTYTGKYAKRIH
                     YLLELLHQRFHGFFVSQTLEAMILGSLTASGMFILRLPFAGTIGVLVAFTALIPVIGA
                     SIGAAIGFILIMTQSMSQAIIFIIFLIILQQIEGNFIYPKVVGGSIGLPAMWVLMAIT
                     IGASLKGIVGMIIAVPLAATLYQVIKDNIQKRQAIQKKQVS"
     gene            24552..25232
                     /gene="radC"
                     /locus_tag="ACGEUI_000303"
     CDS             24552..25232
                     /gene="radC"
                     /locus_tag="ACGEUI_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFF8165169.1"
                     /translation="MYSIKCDDNKAMPRERLMRLGAESLSNQELLAILLRTGNKEKHV
                     LELSSYLLSHLDSLADFKKMSLQELQHLAGIGKVKAIEIKAMIELVSRILATDKTLTD
                     SVLTSVQVAEKMMAALGDKKQEHLVVLYLDNQNRIIEEKTIFIGTVRRSLAEPREILY
                     YACKNMATSLIVIHNHPSGNIEPSSNDYCFTEKIKRSCEDLGIICLDHIIVSYKDYYS
                     FREKSTLF"
     gene            complement(25234..25929)
                     /locus_tag="ACGEUI_000304"
     CDS             complement(25234..25929)
                     /locus_tag="ACGEUI_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFF8165170.1"
                     /translation="MTKPIIGITANQRLNMALDNLPWSYAPTGFVQAVTQSGGLPLLL
                     PIGDEAAAKTYVSMVDKIILIGGQNVDPKYYQEEKAAFDDDFSPERDTFELAIIKEAI
                     TLKKPILGICRGTQLMNVALGGNLNQHIDSHWQEAPSDFLSHEMIIEPDSILYPIYGH
                     KTLINSFHRQSLKTVAKDLKVIARDPRDGTIEAVISTNDAIPFLGVQWHPELLQGVRD
                     EDLQLFRLFVNDF"
     gene            complement(25939..26583)
                     /locus_tag="ACGEUI_000305"
     CDS             complement(25939..26583)
                     /locus_tag="ACGEUI_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFF8165171.1"
                     /translation="MVIDKSIPKATAKRLSLYYRIFKRFHADQVEKASSKQIADAMGI
                     DSATVRRDFSYFGELGRRGFGYDVTKLMNFFADLLNDHSTTNVILVGCGNIGRALLHY
                     RFHDRNKMQIAMGFDTDDNALVGTKTADNIPVHGISSVKERIANTDIETAILTVPSIH
                     AQEVTDQLIEAGIKGILSFAPVHLQVPKGVIVQSVDLTSELQTLLYFMNQNHLD"
     gene            complement(26835..27182)
                     /locus_tag="ACGEUI_000306"
     CDS             complement(26835..27182)
                     /locus_tag="ACGEUI_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFF8165172.1"
                     /translation="MAFEKEIKLKDCKYLYTISPNIKKYTLRDTTFSQTKVGHYQLIR
                     LLEKIPNSGDGFPLKITINKELTGFKLAITDQSGLRLVNIFKTKDNQILQDKFYFLMD
                     SLVEREIFSKTVV"
     gene            complement(27172..28299)
                     /locus_tag="ACGEUI_000307"
     CDS             complement(27172..28299)
                     /locus_tag="ACGEUI_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFF8165173.1"
                     /translation="MTYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKI
                     LRECRQAIARNLGASEQQIIVTSGGTESNNMAIKGYALAHQAKGKHLITTTIEHHSVL
                     HTMAYLEERFGFEVTYLPCQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKDI
                     GNLLKDHQAAFHVDAVQAVGKLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPID
                     PLLHGGDQEGKRRASTENMLGIIGMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLP
                     YYINQGTHYLPHVLNIGFLGYQNTILLTQLDLAGIAVSTGSACTAGAVNPSHVLAAYY
                     GDDSSRLKESIRISFSDQNSIEDVNQLAQTLKNILGGTDGF"
     gene            complement(28296..29276)
                     /locus_tag="ACGEUI_000308"
     CDS             complement(28296..29276)
                     /locus_tag="ACGEUI_000308"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFF8165174.1"
                     /translation="MTERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSNG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVLPYFGYSR
                     QDRVAKPREPITAKLVANMLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAER
                     YSKLGLSGSDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSG
                     KKAILIDDILNTGKTFAEAAKILERSGATDTYAVASHGLFAGGAADVLETAPIKEIIV
                     TDSVKTKNRVPENVTYLSASDLIAEAIIRIHERRPLSPLFSYQPKGKNNA"
     gene            complement(29416..29994)
                     /locus_tag="ACGEUI_000309"
     CDS             complement(29416..29994)
                     /locus_tag="ACGEUI_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFF8165175.1"
                     /translation="MTNLEIEYKTLLTKNEYNRLLSQMKHVTPVTQTNYYIDTKAFDL
                     KANKMSLRIRTFVNSAELTLKVPEKVGNREYNVPLFLEQAKDMIKHGNLPESTALDII
                     ISKGIKPSALVTFGNLTTVRRETVIPIGKLALDYNLYANTKDYELELEVSDALQGKID
                     FDSFLSEHHITFKYAKSKVARCINTLKKFNDK"
     gene            30082..30753
                     /locus_tag="ACGEUI_000310"
     CDS             30082..30753
                     /locus_tag="ACGEUI_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFF8165176.1"
                     /translation="MTLDWEEFLDPYIQTVGELKIKLRGIRKQYRKQNRYSPIEFVTG
                     RVKSIESIKEKMILRGVIEENIAQDIQDIAGLRIMVQFVDDVEEVLALLRQRQDMTIV
                     YERDYIRNMKSSGYRSYHVVVEYPVDTIEGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YGGDFPEEIKKRLEVTAKIALELDEEMRKIREDIREAQLLFDPVTRNLSDGVGNSDDT
                     DELYR"
     gene            30728..31564
                     /locus_tag="ACGEUI_000311"
     CDS             30728..31564
                     /locus_tag="ACGEUI_000311"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFF8165177.1"
                     /translation="MTQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSK
                     KNPDIVISIGGDGMLLSAFHMYEKELDKVRFVGIHTGHLGFYTDYRDFEVDKLIDNLR
                     KDKGEQISYPILKVAITLDDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGD
                     GISVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIE
                     LVPKRLGIYTISIDNKTYQLKNVTKVEYFIDDEKIHFVSSPSHTSFWERVKDAFIGEI
                     DS"
     gene            31561..32466
                     /locus_tag="ACGEUI_000312"
     CDS             31561..32466
                     /locus_tag="ACGEUI_000312"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8165178.1"
                     /translation="MRFEFVADKRIKVKTLLKSYDVSKGLLAKIKYKGGNILVNGIEQ
                     NAIYLLQVGDVVTIDIPNEEPFEKLEAIPFDLDIVHEDDHFLVINKPIGFASIPSAIH
                     SNTIANFIKAYYVDNHYLDQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQTRSIE
                     KRYFALVSGNGMLPDEGDIIAPIGRSKDSIITRAVDPMGKYAKTSYKVVARYSENVHL
                     VDIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRLDLGITRQALHCHYLNFKDPFTESD
                     CSCAIHLTDDFDSVIIGLQKKDRGY"
     gene            32470..33465
                     /gene="pta"
                     /locus_tag="ACGEUI_000313"
     CDS             32470..33465
                     /gene="pta"
                     /locus_tag="ACGEUI_000313"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFF8165179.1"
                     /translation="MSIRSLFGGLREKILGKNMKIVFPEGNDERVVRAAARLKFEGLL
                     EPIILGQSEEVRNLLTKLGFADQDYTIINPNEYADFDKMKEAFVEVRKGKATLEDADK
                     MLRDVNYFGVMLVKMGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRE
                     NTSERYVFADCAINIDPTAQELAEIAVNTAETAKIFDIDPKIAMLSFSTKGSGKAPQV
                     DKVREATEIATGLNPDLALDGELQFDAAFVPETAAIKAPDSAVAGQANTFVFPDLQSG
                     NIGYKIAQRLGMFDAIGPILQGLNKPVNDLSRGSSAEDIYKLAIITAAQAIESQG"
     gene            33592..34364
                     /locus_tag="ACGEUI_000314"
                     /pseudo
     CDS             33592..34364
                     /locus_tag="ACGEUI_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(34558..35247)
                     /locus_tag="ACGEUI_000315"
     CDS             complement(34558..35247)
                     /locus_tag="ACGEUI_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HFF8165180.1"
                     /translation="MKNAISILALLTPENQLEETFIRELSHHFSHLFEVTILTSKANI
                     QSNQLSTFQGIFSFHEHDIDLPTLYFKTSQHGQGFLVTESVFDQATAVLSLSQYLTGF
                     YQKFDGHFLQYLPLQARLSDANGNIIVDNHTFNGSFLPTTDKEIEDWILAELRLSDNP
                     CKTFLLPSGSLDHIYMQHYQALKNPQGQLVGVLDTVQDIKPLLNQYLEETGQAIVGWS
                     DVTSGPSILDH"
     gene            35667..36395
                     /locus_tag="ACGEUI_000316"
     CDS             35667..36395
                     /locus_tag="ACGEUI_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8165181.1"
                     /translation="MIRFNNVSKTFGQTKVLQEQTFQINDREFFVLVGPSGSGKTTLL
                     KMINCLIEPSSGDILLNNVPQTELDLREMRLSIGYVLQQIALFPNLTVAENIAIIPEM
                     KQWSAEEIRQKTEELLDKVGLPAKDYLDRYPSDLSGGEQQRIGIVRAIISHPKILLMD
                     EPFSALDPISRKQLQELMLSLHKEFDMTIVFVTHDIDEAIKLGDRVAILNEGEIVQLD
                     RPEMIKTHPANAFVVNLFGGDEHA"
     gene            36388..37914
                     /locus_tag="ACGEUI_000317"
     CDS             36388..37914
                     /locus_tag="ACGEUI_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFF8165182.1"
                     /translation="MPSLFVTFQNRFNEWLAALGEHLQISLLSLMIALLIGVPLAALL
                     SRSKRWSDIMLQVTGVFQTIPSLALLGLFIPLMGIGTLPAVTALVIYAIFPILQNTIT
                     GLNGIDPSLVEAGIAFGMTKWERLKTFEIPIAMPVIMSGVRTSAVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNANLILIGAISSALLAIIFNSLLQYLEKASLRRIMISFGITLL
                     ALLASYTPMALSQFSKGKDTVVIAGKLGAEPDILINLYKELIEDQSDISVELKSNFGK
                     TSFLYEALKSGDIDMYPEFTGTITSSLLRDKPPLSNDPKQVYEDAKKGIAKQDKLTLL
                     KPFAYQNTYAVAMPEKLAKEYQIETISDLKAHADTLKAGFTLEFKDRADGYKGMQSQY
                     GLQLSVATMEPALRYQAIQSGDIQVTDAYSTDAEITKYHLKVLKDDKQLFPPYQGAPL
                     MKTSLLTKHPELKGILNQLAGKITEKEMQDMNYEVSVKGADANKVARDYLLKTGLIQK
                     "
     gene            38192..39175
                     /gene="guaC"
                     /locus_tag="ACGEUI_000318"
     CDS             38192..39175
                     /gene="guaC"
                     /locus_tag="ACGEUI_000318"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFF8165183.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIITSRSQADTSVTLGKYQFKLPVI
                     PANMQTIIDETIAEQLAKEGYFYIMHRFDEDSRKPFIKRMHEQGLIASISVGVKACEY
                     EFVTSLKEDAPEFITIDIAHGHANSVIDMIKHIKTELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHFESPGKTVEVDGETFKEYYGSASEYQKGEHKNVEGKKIL
                     LPTKGHLSDTLTEMQQDLQSSISYAGGKDLDSLRHVDYVIVKNSIWNGDSI"
     regulatory      39298..39393
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            39480..40061
                     /locus_tag="ACGEUI_000319"
     CDS             39480..40061
                     /locus_tag="ACGEUI_000319"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFF8165184.1"
                     /translation="MQLLEERILTDGNILGENILKVDNFLTHQVDYRLMKAIGKVFAQ
                     KYAEAGITKVVTIEASGIAPAVYAAEAMDVPMIFAKKHKNITMTEGILTAEVYSFTKQ
                     VTSTVSIAGKFLSKEDKVLIIDDFLANGQAAKGLIEIIGQAGAQVVGVGIVIEKSFQD
                     GRRLIEDMGIEVTSLARIKNFENGNLNFLEADA"
     gene            40061..41344
                     /locus_tag="ACGEUI_000320"
     CDS             40061..41344
                     /locus_tag="ACGEUI_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFF8165185.1"
                     /translation="MTHSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSA
                     RELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVLGCAFQSVAPLSIIGAQQGSGAM
                     FGALIASGIYVILVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTA
                     QSMMLSLLTIVIILLVQKFTKGFVKSISILIGLVAGTLVSAMMGLVDTTPVVEASWIH
                     VPTPFYFGMPTFEITSIVMMCIIATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSE
                     GIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILVVIGLLPKFGAMAQM
                     IPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIAAVSISAGLGFNGTNLFAS
                     LPETAQMFLTNGIVIATLTSVVLNLVLNGKDKQDE"
     gene            complement(41408..42346)
                     /locus_tag="ACGEUI_000321"
     CDS             complement(41408..42346)
                     /locus_tag="ACGEUI_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFF8165186.1"
                     /translation="MFVTQQLKLMGTVIDIQIESDKACQQLSRVIDLLYTYKNRFSAN
                     DSNSELMAINQAAGVKPVSVHSDLFNLIQIGKAHSLSTPSNLNIAIGPLVQAWRIGFE
                     DARVPSHNLISQQLALTDPRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYL
                     IEDGIDSALINLGGNVRVHGPNPKSPDKTFRIGIQKPDAKRGQHLGVIKVNNHSVVTS
                     GIYERQFTSKGKQYHHILDRQTGYPIETDMLSLTIMAPSSFYCDIWTTRLFGLDSSMI
                     ITLLNTFDNIEGLLVTRKHHVLMSNGLRHYFQPYYH"
     gene            complement(42399..42584)
                     /locus_tag="ACGEUI_000322"
     CDS             complement(42399..42584)
                     /locus_tag="ACGEUI_000322"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HFF8165187.1"
                     /translation="MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGEMKQKS"
     gene            42722..43291
                     /locus_tag="ACGEUI_000323"
     CDS             42722..43291
                     /locus_tag="ACGEUI_000323"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFF8165188.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     GFGIVSSRIGMRREAIPISNDMDIFTFIAQLEEKPYCVLIDESQFLSKQNVYDLARVV
                     DELNVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPDI"
     gene            43326..44405
                     /gene="prfA"
                     /locus_tag="ACGEUI_000324"
     CDS             43326..44405
                     /gene="prfA"
                     /locus_tag="ACGEUI_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFF8165189.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMAKEELKESKAAKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTKKLESLNN"
     gene            44405..45244
                     /gene="prmC"
                     /locus_tag="ACGEUI_000325"
     CDS             44405..45244
                     /gene="prmC"
                     /locus_tag="ACGEUI_000325"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFF8165190.1"
                     /translation="MNYATLIRTYEDKLEQIDEDRENLAYVFREIKEWSSLDMLIHQN
                     QAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDLKLAVDKRVLIPRPETEELVDMI
                     LAENLDAPLNVLDIGTGSGAIAISLKKERPNWQVTASDISRAALDLAKANADAYQLDI
                     TFIESDVFSLISETFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRK
                     IIEQADNYLTKEGKLYFEIGYKQAEGIKDMLQAYFPQRHIRAVTDIFGKERMVVVDYG
                     VSS"
     gene            45228..45818
                     /locus_tag="ACGEUI_000326"
     CDS             45228..45818
                     /locus_tag="ACGEUI_000326"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFF8165191.1"
                     /translation="MEYLASIIESGDALVLPTETVYGLFAKALDEKAVNAVYDLKQRP
                     RDKAMNLNVADFNSILAFSKEQPRYLKKLYQAFLPGPLTIILKANDQVPYWINSGLST
                     VGFRLPSHPITAALIQKTGPLIGPSANLSGKASGRVFDHIMQDFDFQVFGYADDPFLT
                     GKDSTILDLSGERAVILRQGAITKEELLANVPELRF"
     gene            45836..46288
                     /locus_tag="ACGEUI_000327"
     CDS             45836..46288
                     /locus_tag="ACGEUI_000327"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8165192.1"
                     /translation="MLRPLSKTDCPMLQEINAKALGYLVSLDLLERQYERLIEDCHHY
                     FLAYADKDTNQLLGYVHAERYETLYASDGLNLLGLAVLPAYQRRGIGSALLRALESQA
                     RQEGIAFIRLNSASHRKEAHAFYRNLDYADDKTQLRFIKNLQGDDNDF"
     gene            46278..47534
                     /gene="glyA"
                     /locus_tag="ACGEUI_000328"
     CDS             46278..47534
                     /gene="glyA"
                     /locus_tag="ACGEUI_000328"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFF8165193.1"
                     /translation="MIFDKGNVEDFDKELWDAIHAEEERQEHHIELIASENMVSKAVM
                     AAQGSVLTNKYAEGYPGNRYYGGTECVDIVETLAIERAKKLFGAAFANVQAHSGSQAN
                     AAAYMALIEAGDTVLGMDLAAGGHLTHGSPVNFSGKTYHFVGYSVDTDTEMLNYEAIL
                     EQAKAVQPKLIVAGASAYSRSIDFEKFRAIADHVGAYLMVDMAHIAGLVAAGVHPSPV
                     PYAHIVTSTTHKTLRGPRGGLILTNDEALAKKINSAVFPGLQGGPLEHVIAAKAVAFK
                     EALDPAFKDYAQAIIDNTAAMAAVFAQDDRFRLISGGTDNHVFLVDVTKVIANGKLAQ
                     NLLDEVNITLNKNAIPFETLSPFKTSGIRIGCAAITSRGMGVKESQTIARLIIKALVN
                     HDQETILEEVRQEVRQLTDAFPLYKK"
     gene            47541..48518
                     /locus_tag="ACGEUI_000329"
     CDS             47541..48518
                     /locus_tag="ACGEUI_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFF8165194.1"
                     /translation="MLDSYIKRIVIHQFSPNDTELLLSDRLVSITPRIDEYFRKKLAK
                     VFSDEAKRGQFEANNTFFTTIGDDLLETSVTIAQLWKEAFVISEDQKTNDLVFVQFDK
                     DGEPFFAFLRIALKEQFAHLSDNYEHPFTVTQNNLPSPTQTPDEALVINLKSGQYYLI
                     EKRVKHNGSFANYFSEHLLKVTPEQSVKKSIKMIEQTAQKIAEHFNQDDFTFQSKMKS
                     TLFKQLEADDVLSPEKLADQLFDDNLTARLTFVDQVKDVIPEPIKISDIEHSRQIKKL
                     ENQKLSLSNGIELTVPNAIYQDAEAVEFLLNDDGTYSILIKNIEDIKTK"
     gene            48519..49118
                     /locus_tag="ACGEUI_000330"
     CDS             48519..49118
                     /locus_tag="ACGEUI_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFF8165195.1"
                     /translation="MFRLLKRACSFLLLFVIYQSFVIHHNVQRVLAYKPMVEKTLAEN
                     DTKANVDLVLAMIYTETKGGEADVMQSSESSSGQKNSITDSQASIEHGVNLLSHNLAL
                     AEEAGVDSWTAVQAYNFGTAYIDYIAEHGGQNTVDLATTYSKTVVAPSLGNTSGQTYF
                     YYHPLALISGGKLYKNGGNIYYSREVHFNLYLIELMSLF"
     gene            49128..50852
                     /locus_tag="ACGEUI_000331"
     CDS             49128..50852
                     /locus_tag="ACGEUI_000331"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8165196.1"
                     /translation="MKRLRPYVKGYLKESILGPLFKLLEALFELLVPLLIANMIDISI
                     SQHNSQGILRVVLTLFGLATIGLLLSVTAQYFSSKAAVGFTRQMTDDLFKKIMFLSKE
                     DQDHLGYASLLSRLTSDSFQIQTGINQFLRLFLRAPIIVCGAMVMAYWISPSLTLWFV
                     MMVIVLLTLVFVMSHLLGPLYLLIRRETDHLVRLTSQQLQGIRVIKAFNQTQKELQAF
                     KQQNMLLSRHQYQAATLANVLNPMTFLVVNLTLLILIWQGSWQVAHRSLSQGMLVALI
                     NYLLQILAELLKMTMLMGTINQSVTAAKRINQVFVLADEAPLPLLKDGPISTHLLTIR
                     HLTFTYPGAAEPSLYDIQLSADQGEWIGIIGGTGAGKTTLIDLICQTYSQYSGEISLN
                     WQGEVPKTLTEWRNVIALVPQKAQLFKGTIRSNLLLGQSMPISDEELWRALELAQAKE
                     FVAALPEQLEAPVEAFGRHFSGGQRQRLAIARALLKPKPILILDDASSALDNETRGRL
                     FKALKEELSDVLVILVTQSIKNLQFADKILVLEQGHQLDFASHDQLKVSNALYQEMLA
                     LYVKGEQK"
     gene            50849..52576
                     /locus_tag="ACGEUI_000332"
     CDS             50849..52576
                     /locus_tag="ACGEUI_000332"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8165197.1"
                     /translation="MIKTDHHLLKRVLQDLLKKPLPVCILVIASFVQVGLSVYLPVLI
                     GKAVDMSLSVNSWQTLKWLLGQMLVIIVVNTLIQWVMPLVYSRLLYQYSQQLKDKLLE
                     KIHRLPFAYLDRQTIGDLVSRVITDTEQLINGLQMVFNQFILGLLTILCTIIAMAQID
                     WLMLILVLVLTPSSLFLARFIAQKSFHYAQAQTKSRGNLAQFTEEILRQEGLVQLFNA
                     QEQSICDYHVLNKTYCEASQKAIFYASTVNPATRFINSVIYALLAGLGAVRIMAGLFS
                     VGQLTTFLNVVVQYTKPFNDISSVLAEIQSSLACAQRLYDLLDIEIKEQEHFLTFKAS
                     AVKGQIDFEEVSFSYQKDRPLLKDINFSVPAGSKVAIVGPTGAGKSTLINLLMRFYEL
                     DAGSIKLDKVPIKCYAKEELRSITGIVLQETWLKDATVHELIAYGSEEASRDEVVAAA
                     KAAHAHFFIMQLPKTYDTYLSASDDALSQGQLQLLAIARMFLKKPKVLVLDEATSSID
                     IRTEAVIQEALKELMRGRTSFIIAHRLSTIQSADLILVMDQGRLVEWGTHASLMSKNG
                     CYARLQKIE"
     gene            52817..54187
                     /gene="nox"
                     /locus_tag="ACGEUI_000333"
     CDS             52817..54187
                     /gene="nox"
                     /locus_tag="ACGEUI_000333"
                     /EC_number="1.6.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H2O-forming NADH oxidase"
                     /protein_id="HFF8165198.1"
                     /translation="MSKIVVVGANHAGTACIKTMLTNYGDANEIVVFDQNSNISFLGC
                     GMALWIGEQIAGPEGLFYSDKEELESLGAKVYMESPVQSIDYDAKTVTALVDGKNHVE
                     TYDKLIFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIAKLENKDI
                     KRVAVVGAGYIGVELAEAFQRKGKEVVLIDVVDTCLAGYYDRDLTDLMAKNMEEHGIQ
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTTLGNGKIDLFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATRDTNYIALASNAVRTGIVAAHNACGTDLEGIGV
                     QGSNGISIYGLHMVSTGLTLEKAKRLGFDAAVTEYTDNQKPEFIEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDVSMGIHMFSLAIQEGVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(54346..55329)
                     /locus_tag="ACGEUI_000334"
     CDS             complement(54346..55329)
                     /locus_tag="ACGEUI_000334"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFF8165199.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVTQNIAQELGIIDIFKEK
                     TQGDAEDLSHALAFTSPKKIYAADYSDCHDADLVVLTAGAPQKPGETRLDLVEKNLRI
                     NKEVVTQIVASGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AAKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVGLYDWLQANRDIDEQGLVDLFIS
                     VRDAAYSIINKKGATFYGIAVALARITKAILDDENAVLPLSVFQEGQYEGVEDCYIGQ
                     PAIVGAYGIVRPVNIPLNDAELQKMQASANQLKAIIDEAFAKEEFASAAKN"
     gene            55520..58006
                     /gene="gyrA"
                     /locus_tag="ACGEUI_000335"
     CDS             55520..58006
                     /gene="gyrA"
                     /locus_tag="ACGEUI_000335"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFF8165200.1"
                     /translation="MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYDTGKGSIVLRSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRLEGITAVRDESSREGVRFVIEIRREASATVILNNLFKLTSLQTNFSFNMLAI
                     ENGVPKILSLRQIIDNYISHQKEVIIRRTRFDKDKAEARAHILEGLLIALDHLDEVIA
                     IIRNSETDVIAQTELMSRFDLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LSDILAKPERIITIIKEEMDEIKRKYANPRRTELMVGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDDFVRELISTSTHDTLLFFTNFGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIINARKEETAGKSFFFTTKQGIVKRTE
                     VSEFNNIRQNGLRALKLKEGDQLINVLLTSGQDDIIIGTHSGYSVRFNEASIRNMGRS
                     ATGVRGVKLREDDRVVGASRIQDNQEVLVITENGFGKRTSATDYPTKGRGGKGIKTAN
                     ITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQTGRATLGVKIMKLDADAKI
                     VTFTLVQPEDSSIAEINTDRENSISKNKDN"
     gene            58026..58775
                     /locus_tag="ACGEUI_000336"
     CDS             58026..58775
                     /locus_tag="ACGEUI_000336"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HFF8165201.1"
                     /translation="MVKKQKRRKIKSMSWARKLLIAVLLILGLALLFNKPIRNTLIAR
                     NSNKYQVTKVSKKQIKKNKEAKSTFDFQAVEPVSTESVLQAQMAAQQLPVIGGIAIPE
                     LGINLPIFKGLGNTELIYGAGTMKEEQVMGGENNYSLASHHIFGITGSSQMLFSPLER
                     AQNGMSIYLTDKEKIYEYIIKDVFTVAPERVDVIDDTAGLKEVTLVTCTDIEATERII
                     VKGELKTEYDFDKAPADVLKAFNHSYNQVST"
     gene            58858..59271
                     /locus_tag="ACGEUI_000337"
     CDS             58858..59271
                     /locus_tag="ACGEUI_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8165202.1"
                     /translation="MKLNAIHHVAIIVSDYHLSKDFYVNKLGFEIIRENYRPDKHDYK
                     LDLSCGRIELEIFGKVTSDPNYQAPPKRVSEPEFKSEACGLRHLAFRVTNIESYVDDL
                     KSLGIPVEPIRHDDYTGEKMTFFFDPDGLPLELHE"
BASE COUNT        18340 a        10130 c        12275 g        18723 t
ORIGIN      
        1 ttttattgaa aactattggt ataaatgtat ctctcagact acttattttg aatagtttaa
       61 tttatatcat ttttgcctat gtaacctatc tgatcaatca tattattagc tatatattta
      121 gtacaggtgt ggcattaggt atttcaatgg taggtgttat tgctgcagca ttttgtgaaa
      181 cgagccttgg tgataaggta tggtttctta ttccatgggc atggctttta agaatatcag
      241 atactctata taaccaacag aaaatggcaa ttgttccact tattgttata ttttttgttt
      301 catgcacagt agctttactt catatacgaa tatttaaaag gtggaatcaa gactgtttga
      361 caagtagtta aatacatcta ttttgtttac ctagcaataa atatgatttt ttaagttata
      421 aatactatct aacttgttta actaaaaaat gactataaaa tttcaatgga ttactagctt
      481 atatttaata gtcttgtcca aataggacaa ttaaacattt tttaatcaaa tctcctttat
      541 actgatatta ggagaatgtt agatgtatcc gcgtattagg aatttgagag aagataacga
      601 tttcactcaa aagtttgttg caaatttact ttccttctct catgcaaatt atgcaaaaat
      661 tgagagagga gaggttgcct taatggcaga tgttcttgtg cagttttata aactctataa
      721 cgtcagtatt gattatttat tagggcaaac agattatccg tatcgttttc ataataaata
      781 aaagtctcgc cttgatttgt tcattgacaa ctgaataaac cgaggtggga ctttcttgtt
      841 tgtcgagcaa tttagtgaga tacgccgtga taggagcgat aattattagc tataaaatag
      901 agtggttctc tatctgccat gaccacaaat agggacaatg atacttctga acgttcaggc
      961 tgccagcgta aaaggccagt gggtgagatt cccatgagtg atttaaccag tcatacccac
     1021 ggaggttgta atttttttga aaggagtgga ggtgtgaaaa tagtaacata taaaaagctt
     1081 gatgaatatt ggttttctcg aacatactgc aagaaatatt attagagaag caaaaagaat
     1141 agctataaaa aagttgaaga agctcgcgaa cttgaacaaa atgcggtaca attaagtaaa
     1201 tcgccctttg acaatagaag acttggtatt gctcctaaag atgaagaaag tttagaaagt
     1261 gagaattaaa gatgactata aaaaatcaaa aaaagtacaa aggtgtttat tgtgataaga
     1321 atgggaagat attttatcaa gctgatcttg gtgtagatcc agtaacaggt aaacgagttc
     1381 aaaagaaagc cagaaagaat tagtatggaa agccatttgg tacaatgaaa gaagcgtatg
     1441 atgaactggt tcggattagg tatgggttta gtaataatct tagtattgaa aatgacaata
     1501 tatcgtttga aaactatatg aatactattt acctaagagc atataagcaa aaggttcagg
     1561 ctgtaactta tgaaatagcc ttaccacatc ataagttatt tattcagtat tttgattcta
     1621 aacttttaaa gggaataaca gcttgagatt gtgaatcttt tagtttacat attattgaaa
     1681 attatgcaaa gaatttatgg tcgcgattca aagcttgcat ggggtatgct gaaaggttag
     1741 actatatttc aaaagtgcca tgtaaggctt tggataatcc taaagggaaa catcccgaca
     1801 ctccattttg gacttatatt gagtttcaga attttataaa atcgtttgat ttgcaagatt
     1861 atgaagagct acaaagattt acaactattt ggctatatta tatgacaggt gttcgtgtaa
     1921 gtgaaggttt atcattatga tgggaaaata tcgatttcga acaaaaattc ttgaaagttc
     1981 atacaacctt ggaaaaggat gaaaatggaa attggtatcg taaagaccaa acaaaaacgc
     2041 cagcaggaga acgtcttatt gagttagatg atgtgacaat agtagtattg gaaaattgga
     2101 gaagaaatca agtagtcaat actgacacag atttcattat ttcacggttt ggtgaaccat
     2161 tttgtaaatc aacgatttgt cgagttatta agcacaaagc tcaaagtatt ggagttcccg
     2221 taataactgg aaaaggattg cgacatagtt atgcttccta tcttattaat gttttgaaaa
     2281 aagacattct gtacgttgca aagtgtatgg gacatgctga taagtctaca actttgaata
     2341 catatagtca ctgatttaat gcattagata aaacagtatc tgaagaaata acccaaaatg
     2401 taataagttc aggattagat tccattttat accaaaattc ctgccaaata actaattgat
     2461 ataacgaagc ttattatatc gcccatataa caggatgtgt tattgttaca gggtacttgc
     2521 tttatgagct tgtttaagct actaaaggtg tgtgccacta tacttccttc caaatttctg
     2581 agaatttttg ttataatagt aactatgaaa gatataatga taaaaaggtg ggttatcctt
     2641 ttttttatgg tattaacttg ttcagggttt ggtaaaacag ttctcgcagc tgattttcaa
     2701 gtgggagcag aacatgcgat tgtcgtagag gcagactctg ggagagttct gtatgagaaa
     2761 gacgctaaga cgccagatgc tattgcctct ttgaccaaat tggtgacggc ttatctggtt
     2821 ttagataagg ttaaatcagg ccagctgcaa ttatcagatc aagttaatct ctcagattat
     2881 gccttcgagt taacaaaaga taggtcttta agtaatgtac cttttgacaa aaaaacttat
     2941 tcagttcaag acctgttgac agctactttg gtagcaagct caaatagcgc tgctattgcg
     3001 ttagccgaaa aagtggcggg ctcagagctt cattttgtaa atcaaatgag agaacagcta
     3061 tctcattggg ggattacttc tggtaaaatc ctcaacgctt caggactgcc gaatgaggtg
     3121 ctaaaggacc atcgttatcc tggttctgcg ttggaagaag agaatatgtt gagtgctcag
     3181 gatgttgcca ttgtgacgat gcatttactg gaagattttc ctgagatttt agagatcact
     3241 aaacaaacag aagttgattt tgctggtaat tctattaaaa gttttaatca actcttacca
     3301 ggtatggcta agggtagagc aggagtggat gggctaaaga caggaacgac agatttggca
     3361 ggtcattgcc ttgtggtaac ttctattgaa aatggtatga ggctgattac cgttattcta
     3421 aatgctgatg gatctgataa aaaccaaaac acgcggtttg agcaagcaaa tcgcttgttg
     3481 gactatgtgg ctagaactta ttgtcgtcgt aagattctta aaaaaggaag tcttgtctcg
     3541 gagcgatcgc tccctattca agatggccaa gtaaaagaac taccgatatc tgttgccgaa
     3601 gatgtaacca tcattttgca acagggtgaa caagttccca aacctaaaca atttatgata
     3661 tcagagactt ctttattagc accgattact aaaggtgaag tggttgctta tttgacgtct
     3721 ccaagaataa cagatcagtc agtgaggtat ttaaaggaac ctaagcgtat tcctcttaaa
     3781 gctagccaat ctctaaaaaa agcctctgac ttacagctgt ggtggcgaga ttttcttgaa
     3841 aaaagacgtt aaagataaga ataaaataat tttaaaacaa tcaaaaaaag acagttatga
     3901 aagaattttt tcataactgt ctttttagtt tacagtgagt atgatttgga atgataaaaa
     3961 aattgatgtt atggttccat tgtttgtaaa agattttcag gactcatgtt tgggaggata
     4021 cgaatccggt taagagaaag aagtccatta gccgcgctag ctattccttc gttagtggta
     4081 acacctaatt tatagttaag tctagctgca atttgtaagg tagtgtcatt atatctacca
     4141 gatggataag caatggcaat agtgttttgg tttaattgct tatcaagata gtcctttgaa
     4201 tctttcattt cagttgtttg aacatcagga ctagcctgct ctagatcagg atgattcact
     4261 gtatgatctt ggaaagacat accgacttgt ttcatttctt tcatctgctt aagagtcaga
     4321 ttggcagcgc ttcccatttc ggttagtcct gtaataacat tattggtggc tttagcatca
     4381 tattttttta agattgggta agcaacattg tagaaatcaa tcatactgtc atcaaatgtt
     4441 agccatacaa cttttttagc aggcaattca ttatttgaaa gagcgcggta gacctcttca
     4501 ggactcaaaa aatagtagcc ctcgtctttc attttttgaa gttgttgatc aaagagatct
     4561 gggttgacaa tcaaattagc attagccgtc tcttcagggg acattacatg aatagcatga
     4621 tacattaaaa ttgggatctt gacaggtgtt tcttgcttgg tccaagtcaa tttagaatgg
     4681 gccttttttt gagattttga ggaatcgaaa tgagtcttct taagttttgt cgtttctttt
     4741 tttgccgtct ttttgtgatg aaaaagactt attttaggta caaatcgaga gacatctgac
     4801 aagccgaatt tgttagaccc ttgatataaa tagaaaatga gggtcaaacc aagaatgcag
     4861 gcaaataata gtataaggtt aatgagagtg aaggcttttc ttttttggcg ccgtttatgt
     4921 ctattgttca ttacttgctc ctttttttga tataagtacc tctattgtaa cattttttag
     4981 agaaaagaag gcttgaagtc agcctttttt cttgtttttt ggaagaacgt gtattaagga
     5041 gtaaaaaaat tattgtcaca gataacactt taggaggtag caaagactat gtggggctgg
     5101 tattcttatc acgtaaaaaa agcaatgaag ataaaagtgt agtgtacttt agaacttatt
     5161 tgagtgtttg gtgaggataa taaaaaaaag ggtactactc taggttatct gctgtcactt
     5221 ttttttatat atggtatact aaagatagat gtgaggtatg aaatgactta tgaagagact
     5281 ctagaatgga ttcatgatca cttagttttc ggcatcaagc caggcttaaa acgtatgctt
     5341 tgggttcttg gacagttagg aaatccccaa aaaaatgtca agggagtgca tatcgttgga
     5401 actaacggaa aaggttccac ggtcaatcat ttacaacaca tctttacgac agctggttat
     5461 gaagtgggga cctttacctc tccttatatt atggatttca aagaacgtat tagcataaat
     5521 ggtcgaatga tttcagaaaa agatttggtt attgctgcta atcgtattcg tcctttgaca
     5581 gagcgtctag ttcaagagac tgattttgga gaggtaactg aatttgaagt gattactttg
     5641 atcatgttcc tctattttgg tgacatgcat cctgtagata ttgctattat tgaagcaggc
     5701 cttggcggtc tttatgattc gaccaatgtt tttcaagcaa tggttgtggt ctgtccctca
     5761 attggtttag atcaccaagc tattttgggc gagacttacg ctaatattgc cgcccaaaaa
     5821 gctggtgttt tagaaggggg cgagaccctt gtttttgccg ttgaaaaccc gtcagctcga
     5881 gaggtttttt taacaaaagc tgagcaggtt ggtgcttcaa tctgggaatg gcaagagcag
     5941 tttcaaatgg ctgagaacgc atctggctat cgttttacta gtcctttagg agttatttca
     6001 gatattcaca tcgctatgcc aggacaccac caagtgtcta atgcagcttt agctattatg
     6061 acttgcttaa ccttgcaaga ccgctatcct aggctaactc cagatcatat aagggaaggt
     6121 ctagcaaata gtttgtggct aggccgtacc gaattattag caccaaatct aatgattgat
     6181 ggggctcata acaatgaaag cgttgctgct ttggtagccg tcttaaaaaa taactacaat
     6241 gataaaaagc ttcatatttt gtttggtgcg atcgacacca agcctattgc agatatgttg
     6301 gttgctcttg agcaaatagg tgacttgcag gttactagct ttcattaccc taacgcctat
     6361 ccattggaaa aatacccaga acgttttggt agggttgctg atttcaaaga tttcttggcc
     6421 ttgcgtaagc atgctaaagc agatgacttt ttcgtgatta cagggtcact atattttatt
     6481 tcagaaatta gacagtattg ggaaaaacat attgaaaaaa gcgttctttt aacccattaa
     6541 aaaagagaaa atgaaaagga aaaaactaat gaaaagggaa agactaatgt ctattaataa
     6601 ggaaaaagca gaagcagcca tttatcagtt tttagaggct attggtgaaa atccaaatcg
     6661 ggaaggtctt cttgacacgc ctaaacgggt tgctaaaatg tatgcggaga tgtttttggg
     6721 actgggaaaa gatcctaaag aggagtttac ggctgtcttt aaagaacaac atgaggatgt
     6781 ggtgattgtc aaagacatta gtttttattc tatatgtgaa caccacttag ttccttttta
     6841 tggcaaggct catatcgctt acttgccaag tgatggtcga gtaacaggtt tgagtaaatt
     6901 agcgcgagca gttgaagtag ctagtaaacg acctcaactc caagagcgtt tgacctctca
     6961 aatagcggat gctttagttg aagccctcaa tcctaaaggg actttagtta tggtagaagc
     7021 agagcacatg tgcatgacca tgcgaggcat caaaaagcca ggtagtaaaa ccattaccac
     7081 cactgccaga ggcttatata aggaaagtcg tgctgagagg caagaagtta tttctctaat
     7141 gacaaaagat taggagaact tatgaaaatt ggaaagtttg tgattgaggg caatgcggct
     7201 atcatgggga ttttaaatgt gactccagat tctttttcag atggggggtc ttacactact
     7261 gtgcaaaaag cattagatca cgttgagcaa atgattgctg atggggctaa aatcatcgac
     7321 gttggtggag aatcaacacg tccaggttgc caatttgtaa gcgctaccga tgaaattgac
     7381 cgggtggttc ctgtgatcaa ggccatcaaa gaaaactatg atattctaat cagcattgat
     7441 acctataaaa ccgaaacagc tagagcagct ttagaggcgg gtgccgatat tctcaatgat
     7501 gtttgggcag gtttgtacga cggtcaaatg tttgccttag cagccgagta cgatgcgcct
     7561 atcatcttga tgcataacca agacgaagaa gtttatcaag aggtaacaca agacgtttgt
     7621 gattttctag gcaatagagc acaagcagct cttgatgctg gcgtgccaaa aaacaatatt
     7681 tgggttgatc caggatttgg atttgccaaa tctgttcaac agaatacgga gttattaaaa
     7741 ggattggacc gcgtctgtca gttgggctat cctgtcttgt ttggtatttc gagaaagcgt
     7801 gtcgtagatg ccttgttagg cggcaacacc aaagctaaag agcgagacgg agcgacagca
     7861 gccttgtctg cttatgccct tggaaaaggc tgtcagattg tacgcgtaca cgatgtcaag
     7921 gctaatcaag acattgtggc tgtgttgagc cagttgatgt gaggacttat ggataaaatc
     7981 gtattagaag gttgccgttt ttatggctac catggagcct ttaaagaaga acagaccctt
     8041 gggcaaattt ttctggttga tttagaattg tcagtcgatt tgcaagcagc ttctttgtca
     8101 gaccaattaa cagatacggt ccattacggg atggtgtttg atagtgtccg tcagttagtg
     8161 gaaggggaaa agtttatttt gattgaacgt ttagcaggtg cgatttgtga acagcttttt
     8221 aacgaatttc cgcctattga agccatcaaa gtggccatta aaaaggaaaa cccaccgatt
     8281 gcaggccact ataaggcagt tggtattgaa ttggagagac agagatgacc attgtttatt
     8341 taagtttagg caccaatatg ggggaccgag cagcttactt gcaaaaagct cttgaggctt
     8401 tagctgactt acctcaaaca cggttgcttg ctcaatcatc catttatgaa acgactgctt
     8461 ggggcaagac aggccaggct gattttctca atatggcctg tcaattagat acacaattaa
     8521 ccgcggctga tttcttaaaa gaaacacaag ctattgagca atctcttggt cgtgtgaggc
     8581 atgaaaaatg gggatcaaga actattgaca ttgatatctt gctatttgga gaagaggttt
     8641 atgacacaaa ggaattgaaa gtgccacacc cttatatgac tgagcgtgct tttgttttaa
     8701 tccccttatt ggaattgcag ccagatttaa aattgcctcc taatcataag tttttaaggg
     8761 attatcttgc agctttagat caatcagata tcacactctt ttcagctcaa cagacagagt
     8821 tctagactct gttttcggag taatgcctgc tataagccaa tccataaagt tttgccaaat
     8881 tcatcgttat tttttctaaa atttgatata atagtatcgg ctttatgccg atttttttac
     8941 gtgtataaga agtgataaaa gaaagaagat aactatgata actgaacttc atgggattga
     9001 tattcgagag aatgaacccc taaaacatta cacttacact aaggtaggtg gcccagcaga
     9061 tttcttggct tttcctagaa atcactacga gctatcacgc atcgttgctt atgccaacaa
     9121 agaaaatatg ccttggcttg ttttgggaaa tgccagcaat ctcattgtgc gagatggtgg
     9181 tattcgtggc tttgtcatca tgtttgataa gctaaataca gtgcacttaa atggttatac
     9241 cttagaagcc gaagctggtg ccaatctgat tgaaacaacg aagattgcca aattccatag
     9301 tttaacaggg tttgaatttg catgtggcat tcctggaagc attgggggtg ctgtttttat
     9361 gaatgcgggt gcttatggtg gggaaatatc acatattttc ttatccgcta aggtgcttac
     9421 cccaagtgga gagatcaaaa ccatttcagc tagggatatg gcttttggtt accgtcactc
     9481 tgccattcaa gaaacaggtg acattgtcat ttctgctaag tttgctctta aaccgggtaa
     9541 ttatgatacg atcagccaag aaatgaatcg gttaaatcac cttcgccaac taaaacaacc
     9601 tttagaattt ccatcttgtg gatctgtgtt taagcgcccg ccaggacact ttgcaggtca
     9661 attaatcatg gaagcaaatc ttaaagggca tcggattggt ggtgtggaag tttccgaaaa
     9721 acacactggt tttatgatta atgtggcaga tggcacagct aaagattatg aagatttgat
     9781 tgcttatgtc attgagacag ttgaaaacca ttctggtgtc aggcttgaac cagaagttcg
     9841 gattattggg gaaaacctgt aaccattatt gataattaaa gaaagtaccg tggaggattt
     9901 atgacaattg actaagccaa ttatcacatt taacaatgtt tcaaaaacat ttgaggacag
     9961 tggaacacag gttctaaaga acattaactt tgaccttgaa gaaggaaaat tttacaccct
    10021 gcttggagct tctggctctg gaaaatcaac tattttaaat attatggcgg gcctactaga
    10081 tgccagcagc ggagacattt atttagatgg ggaacgtatt aatgatttgc cgattaataa
    10141 acgtgacatt cataccgttt tccaaaatta tgccctcttc cctcatatga ctgtttttga
    10201 gaatgttgcc tttgctttga agctgaagaa agtggacaag aaagagattg ctaagcgcgt
    10261 gaaagaaacc ttgaaaatgg ttcaattaga agggtttgaa aatcgttcta tccagaagtt
    10321 gtctggtggt caacgtcaac gtgttgccat tgcgcgtgcg attatcaatc aaccgcgtgt
    10381 ggtcttactt gacgaaccac tctcagccct tgatttgaaa ttaagaacag agatgcaata
    10441 tgaattacgt gaattgcaac aacgtctagg cattaccttt gtttttgtta ctcacgatca
    10501 agaagaagcc ttggccatga gtgattggat ttttgtcatg aatgaaggtg aaattgttca
    10561 gtcgggaaca ccagtggata tttacgatga gccaattaat cattttgttg ctaattttat
    10621 tggagaatct aatattatta acggtaccat gattgaagac tatcttgtct cctttaacgg
    10681 gaaagaattt gaatctgtgg acggtggaat gcgccctaat gagcctgttg aagtggttat
    10741 tcgtcctgaa gatcttcaaa ttactttgcc agaagaaggg aaattacaag ttaaggttga
    10801 tacccaatta ttccgcgggg ttcactacga aattattgcc tatgatgaat tgggtaatga
    10861 atggatgatt cattctaccc gcaaagctat cgaaggagaa gttatcggat tagattttac
    10921 ccctgaagat cttcatatca tgcgtcttaa tgagactgaa gaggaatttg atgcccgtat
    10981 tgaagaatat gtggaaatgg atgagcctga agatggattg attaatgcca ttgaggagga
    11041 gcgtaatgaa gaaaacctct agtctttttt cgattcctta cttcttatgg attctctttt
    11101 ttgttgtggc accagtcact ctcttgtttt acaagtcctt ttttgacata gaagggcgcg
    11161 tgaccttagc caattatgaa acctttttta gctcttggac ttatttgagg atgagtgtga
    11221 attctatttt atacgctggt attatcacac tcgtcacgct cttgatttca tatcctacgg
    11281 ctctcttttt aacgcgccta aagcacaagc agttgtggct tatgctcatt atcttgccaa
    11341 cctgggtaaa tttattgcta aaggcctatg cctttatggg aatctttggt caacaaggag
    11401 ggattaacag ctttttaacc tttatgggga ttggcccgca gcaaatcctt ttcacagatt
    11461 tctccttcat ttttgtagcc tcttacattg agctcccttt tatgatgtta ccgattttta
    11521 acgctttgga tgatattgac cataatgtca tcaatgccag tcgcgaccta ggagctagtg
    11581 aatttcaggc cttctcaaaa gttatttttc ccctttcttt aaatggggtt agggcaggtg
    11641 ttcagtctgt ctttatccca agtttgagtc tctttatgtt aacccgtttg attggtggaa
    11701 accgggtgat tacacttggt acagccattg aacaacattt tttgaccacc caaaactggg
    11761 gaatgggatc aaccattggt gtcgtcttga ttttgaccat ggttgctatt atgtggctca
    11821 caaaggagaa aagtaaatga aaaaatttgc caatctttat ttagcgagtg tctttgtttt
    11881 actctacatt cctatttttt atttgatttt ctattctttc aacaaaggtg gggatatgaa
    11941 tggttttaca ggatttaccc ttgagcatta ccaaaccatg tttgaggata gtcgtctcat
    12001 gacaatctta ctgcaaacct ttgttcttgc ttttagtagc gctctactag caacgattat
    12061 tgggatcttt ggagctatct ttatccacca tgttagaggt aagtaccaaa atgccatgct
    12121 atcagccaat aatgtcttga tggtatcacc agatgtcatg attggggctt cctttttaat
    12181 tctttttaca tcattgaagt ttcagctggg catgtcttca gttttattaa gtcatattgc
    12241 tttttcgatt cctattgtgg ttttgatggt attgccgcgc ttgaaagaga tgaatcagga
    12301 catggtcaac gccgcttatg atttgggagc taattatttc caaatgctca aagaagtcat
    12361 gctgccatac ttaacaccag ggattattgc aggttatttt atggccttta cctattcctt
    12421 agatgatttt gcagtgactt tctttttgac tggaaatggt tttactactt tatctgttga
    12481 gatttattcg cgggctcgtc agggaatttc cttggatatc aatgctttgt caaccatcgt
    12541 tttctttttc tccatcctct tagtgatcgg ttattattat atgtcacagg acaaggagga
    12601 aaaacatgcg taaactttat tcctttctag caggagtttt gggtgttatt gttattttaa
    12661 caagtctttc tttcatcttg cagaaaaaat cgggttctgg tagtcaatcg gataaattag
    12721 ttatttataa ctggggagat tacattgatc cagctttgct caaaaaattc accaaagaaa
    12781 cgggcattga agtgcagtat gaaactttcg attccaatga agccatgtac actaaaatca
    12841 agcagggcgg aaccacttac gacattgctg ttcctagtga ttacaccatt gataaaatga
    12901 tcaaagaaaa cctactcaat aagcttgata agtcaaaatt agttggcatg gataatatcg
    12961 ggaaagaatt tttagggaaa agctttgacc cacaaaacga ctattctttg ccttatttct
    13021 ggggaaccgt tgggattgtt tataatgatc aattagttga taaggcgcct atgcactggg
    13081 aagatctgtg gcgtccagaa tataaaaata gtattatgct gattgatgga gcgcgtgaaa
    13141 tgctaggggt tggtttaaca acttttggtt atagtgtgaa ttctaaaaat ctagagcagt
    13201 tgcaggcagc cgagagaaaa ctgcagcagt tgacgccgaa tgttaaagcc attgtagcag
    13261 atgagatgaa aggctacatg attcaaggtg acgctgctat tggaattacc ttttctggtg
    13321 aagccagtga gatgttagat agtaacgaac accttcacta catcgtgcct tcagaagggt
    13381 ctaacctttg gtttgataat ttggtactac caaaaaccat gaaacacgaa aaagaagctt
    13441 atgctttttt gaactttatc aatcgtcctg aaaatgctgc gcaaaatgct gcatatattg
    13501 gttatgcgac accaaataaa aaagccaagg ccttacttcc agatgagata aaaaatgatc
    13561 ctgcttttta tccaacagat gacattatca aaaaattgga agtttatgac aatttagggt
    13621 caagatggtt ggggatttat aatgatttat acctccaatt taaaatgtat cgcaaataag
    13681 cactcaaaaa ggctggtatt tctccagcct ttttaatgta tcgacagatg acgtcaccag
    13741 tacacgcgat aggggcggcc aacctttgta aaaatttgtt ggctgttcag ttgcttattt
    13801 tcttctaaat aagcaatgta tttacggaca gagacatggg ataagtgaca agcggaagct
    13861 aattcttgga tagtaaaagg ttgatcaaag actttgatat tttccataat ccactggaaa
    13921 gtggattcag ataagccttt ttctaacaat tgtttgtttt tagtgtcttt tttgctagtc
    13981 aaacatttca gttggtcaat ttgggcctgc tctagttgct gattggccaa atgttctcga
    14041 tgtgtaacaa actgttggat gctttcttga aagcgttcaa aggtaaaggg cttgatgagg
    14101 tagtcgataa ttcctaaatg aaaaccatct cggataatat tcccatcatt tgcggctgaa
    14161 ataataatga cttcgcaagg aatatgctga gtacgccatt tctccaaaaa ttgaatccca
    14221 ttgccatctg taatatgaat atccagtaaa atgaggtcaa ttgcgtaatc agtaagtatg
    14281 ctttgaacag ctttcataga gtcgctggat ataatgcgat caaaaagatt tagtttttcg
    14341 aggtaattgc gatggataaa atccaccata ggatcatctt caatgattaa aacgttcatg
    14401 ttgccctcct acataaggtg ttgtcactga gaactgtaac tcggttacgg tttgatgttg
    14461 atggaattgg gaatttgtat cggtcaagag ctgttgaaaa taaggtaagt caaaaatagg
    14521 ctgtacccgc tccaaatcaa tccaattgtc tgtcaaggtg taatggctga ttaaatgatt
    14581 ctggtcatgt tgaatactca aaacgagtga tttgggtctc agtgtggtta gaagattagt
    14641 atggataaag cggtaaatca tcaaaccatt attaagttga ttcttggtgg cagaatgagg
    14701 aatctcgctt aaaacatcaa tcttgagatg gacatttaat tcttggtatt tctcttgctc
    14761 tccaatgaga aaactagcta gaagaggctc cttaacaagg acagataagc tagtgagaat
    14821 ttcattttcg ggttccaaaa tactgtctaa ataaattttc aattggtcat aataagcaat
    14881 atccactaag ccataaataa catgcaattg attcatgaat ttatgagttt gggcttgtag
    14941 agcagaagca taggcagtgg tataggctag ctgatccaag gtatcaatag ccttaactgc
    15001 ttctcgcatg aaaataatat ggcctcggtg atcgtttttg acacagatgg gtgaaatggc
    15061 taacaagtag tcttcctcac cataccgaaa gagttgttca tgcccctctt gcaaactaag
    15121 ttttggaaaa tcaggaaaga ggtaattgaa actgttgcca gtaggttttc ccaactggca
    15181 tttccgagca attaattttt gtccagcctg attgcagagc tgtaagatac cagctttatc
    15241 taccacaaat acggcggctt cgatttgatc caacatggca ttacgctctt caaagagctg
    15301 ataaatttca cttggttcta actggtgcag ttgacgcttg agtcgaaaag aaataaaact
    15361 ggtgaccagt aaactaataa gaagacaaag gagtaaagac agggtataat tgcgttttga
    15421 cgttaaagcc acatcattta gggtagttag tttaatgcca accgcaatag caccaatctg
    15481 tttgtcaccg tcaaacacag gtactagata gcgaagtgac tttcctaaag tgcccttggc
    15541 ggtagagata accttttttc ctgctagaac agcctcctca tcacctcctt gaaaaggttt
    15601 tccgatattt ttcgggttag gatgggtgag tcgaatccct ttcatgttca tcacgaccac
    15661 ataatcaagg ttatagatgg aggcaatgga gttggtatag gctgttgttt tagcatttgg
    15721 ttggttgttt aggagtaatt ctttaatcgc ttgatgactt gctaacattt ttccagttga
    15781 cgttagcaaa tgggtttcct gatttttgat ggattggtgc gtatcatgta gcataatccc
    15841 gtaaaataga gaggttgtga caacaatcat agataccaaa atcaacgata aactggccca
    15901 taaacgtagt ggttttttca taaggtctcc tctttattga gtataacacg ataagaaatg
    15961 acctgctagc ttaaattaat taaataactt gaaaaaatta aagtagttaa acaacttaaa
    16021 taaactatgc ttttgacatg ttctctttta tactagcctt atcactaaag gaggttgttt
    16081 catggaaaca ataagcaaga aaatgcctca aaaagacttg tctgagcatt caaaagcatg
    16141 gcaaaatagg cgaataggct ccgttccctt gcctgtttat ctggttttag ctaccttaat
    16201 tttagtcaca ggctggctcc aacaattgcc tgttaatatg ctgggtggct ttgctgttat
    16261 tttaaccctt ggttggctac taggaaccat tggagcgacc attccaggcc tcaaacattt
    16321 tggtggccca gctattttat cactcttagt cccttcgatt ttggtttttt ttaatctcct
    16381 caatcccaat gtcttagaag cgacgaatgt cttgatgaag caagccaatt ttctttattt
    16441 ttatattgcc tgcctggtct gcggtagtat tttaggcatg aatcgaaaaa tcttgattca
    16501 aggtcttttt cggatgatta ttcccatgtt acttggaatg gtttgtgcaa tgggtgtagg
    16561 gactttggtt ggcgtcattt taggattaga ctggcaacat accctctttt atgttgtgac
    16621 gccagtcctt gctggaggaa ttggtgaagg tattttaccc ttatctcttg gttacagtgc
    16681 cattacagga gtcggaagtg agcaattagt ggctcaactg attccagcca ccattattgg
    16741 gaatttcttt gctatcttgt gcacggcttt gctcaatcgt tttggagaga agcacccaag
    16801 ttattctggt caagggcaac tggttaaaat tggtcacagc gaagacatgt ctgatgccct
    16861 aaaagacaat tctggagccc tagatgttaa attaatgggg gcaggcgttt taacagcttg
    16921 ttcacttttt attgctggtg gcttgttaca gcatttaaca gattttccag gaccggtgtt
    16981 gatgattatt ctggcagcct tcttgaaata cctgaatgtt attccccaag aaacccaaaa
    17041 tggtgccaag caactttata aattcatttc cagcaacttt acttttccgt taatggcagg
    17101 gcttggtcta ctttatatcc ccttaaaaga ggttgtggca acccttagct ggcaatattt
    17161 tattgtggtg attagtgtgg tgttaacggt tgtcagtgtc ggtttttttg tctctcgttt
    17221 ccttaacatg agcccagtgg aagcagctat tatttcagct tgtcaaagtg gtatgggtgg
    17281 aaccggtgat gtggctattt tgagtacggc tgaccgcatg aatctaatgc cgtttgccca
    17341 agtcgctacc cgtttaggtg gggctattac cgttatcact atgacagcta ttttaagaat
    17401 tatttttagt taatccgaac caataaaaag agaggtattt cctatgaaaa atcaattagg
    17461 tcaacttgcc cttgaacaag ccaaaacgtt cggcggtaaa ttggaagttc aaccaaaagt
    17521 tgacattaaa actaaacatg atttgagtat tgcctacaca ccaggtgtag cgagtgtgtc
    17581 atctgccatc gcaaaagata aaacattagc atatgactta accaccaaga aaaatacagt
    17641 agccgttatc agtgatggta ctgcggttct tggtctgggg gatattggcc ctgaagcagc
    17701 catgccagtt atggaaggaa aagcagccct ttttaaagca tttgcgggtg ttgatgctat
    17761 tccgattgta ttagatacca aagatactga agaaattatt agtattgtca aggcgcttgc
    17821 accaaccttt ggtggtatta atttagagga tattagtgcg ccccgctgtt ttgaaattga
    17881 gcagcgcttg attaaggaat gtcatattcc tgttttccat gacgatcagc atggaacagc
    17941 tattgtggtg ttagcagcca tcttcaatag tttgaaactt cttaaaaaat cccttgatga
    18001 ggtatccatt gtggtgaatg gtggtggatc tgctggatta tccattaccc gtaaattatt
    18061 agcagcaggt gctacgaaag tgacagtagt ggataaattt ggcattatca atgaacaaga
    18121 agccgctcaa ttagcgccac atcatttgga tatagcgaaa gtcactaatc gtgagtttaa
    18181 atcaggtacc ttagaagatg ctcttgaagg agcagatatc ttcattggcg tatcagcacc
    18241 aggagtctta aaagcagagt ggattagtaa aatggcagcg cgcccagtaa tttttgccat
    18301 ggccaaccct attccagaga tttaccctga tgaagccctt gaagcggggg cttacattgt
    18361 aggtactggc cgcagtgatt ttcctaacca gattaataat gtccttgcct ttccaggtat
    18421 ttttagggga gcattagatg cacgggcaaa aaccattact gttgagatgc aaattgctgc
    18481 cgcaaaaggt attgccagtc ttgtcccaga tgacgctttg agcactacta atattattcc
    18541 agatgccttt aaagaaggag tcgctgagat tgtcgctaaa agcgtcaggt cggtagtcct
    18601 taaatcatag aatatatgaa aaaaaagaga aagttatact ttaaatcaca aaataagtag
    18661 aagcatattt ctagcttcta cttattttgt gattagctaa tatcattatc aataataact
    18721 ttcaaggcgt tatttgcacc agcatgttta aaggtttcat aagctttttc aacttctgaa
    18781 agtttaaaat gatgtgtaat aagtcttgtt gcgtcaatct ttccagtttt caagacattt
    18841 agtagcattt cagttgtatt ggcattaaca agtccagtgt ttaaggtgat atttttaatc
    18901 cataattcat ccaggttgaa atcaactggt ttcccatgaa ccccaacgtt agcaatatgc
    18961 ccacctactg atataatttt ttgacaaata tcaaatgtcg cgggataacc aacacactcc
    19021 atggaaatat cgacaccacg tccattggtt atatcgtcaa tgatagcctt gacttcttca
    19081 ctacttcctg aacaaatcgt gtgtgtggct ccaaatgttt tagctgcttc aaggcgattt
    19141 tgagataagt cgaccataat aatattggct ggagagaaaa attgaacggt tagtagggct
    19201 gcgagtccta ctggaccggc accaacaata caaacattat ctccaggctt gacatgtgat
    19261 ggcaagacac caatttcata tgaagttggt aaaatatcag aaagcatgac aagtgcctca
    19321 tcatcaatcg tatcaggagc atgatagaga ctaccatctg catgtgggat atggacatat
    19381 tctgcttgtg ttccattaat taaatggcca agaatccaac cgccatcttg acaatgtgac
    19441 gaaaggcctc gtttacaata ataacaggta tgacatgatg tgacgcaaga aataatcact
    19501 ttatctccga ttttaaagtt agtaaccgca tcaccgacct ccttaacaat tccaattcct
    19561 tcatgaccaa ggattgttcc ttctttacat gcgggaacat ccccacctaa gatatgcaaa
    19621 tcagttccac aaatagtggt ttttaccaac tggacgatag catctgtagg ctttataatg
    19681 actggttttg gtttgtctat gagctgtaga tttccggttg aaaggtaagt tgctgctttc
    19741 atatttaact tcttataagg ttgagaggta atcaaccaat tcatttttac acagatatta
    19801 taatatttta agaaagggtt ttcaatattt tttaaatcga ttatgataat tttttagatc
    19861 atctttgttg aataacttca tccttattca gaacttaaag ggtactatta taagaatttg
    19921 aaaccgcttt acttaaaccg tgtatttttg tacaataatg attaaggagg tttttagatg
    19981 aaaaaagaat ttaccagtat cctgtttaca gttagttttt gtggaatcat agcattgcct
    20041 gtcgaagcta gtggccctaa agttccttat acacaagaag gtataacggc catttcaaat
    20101 caggcaacag taaaattgat ttctattgct gatatcgcga gcagtttaga aggacaaaaa
    20161 cctataacgg ttagttttga tatcgatgat actttgttat ttactagcca atattttcag
    20221 tacggcaaag aatatataac tccaggctca tttgattttt tgcataagca aaaattttgg
    20281 gacttggtcg ctaaacgggg agatcaggat tctattccaa aagagtatgc aaagcaacta
    20341 atcgcaatgc atcaaaaacg aggagataaa attgtcttta ttactggccg aactcgagga
    20401 tcgatgtata aaaaaggtga gattgataaa acagctaagt cactggctaa agattttaag
    20461 ttagacaagc caatagctat caattataca ggagataaag ctgtgaagcc gtatcagtat
    20521 gataaaacat attatataaa gaaaaacggc tcacaaattc attacggaga tagtgatgaa
    20581 gatataaatg ctgccaaaga ggcaggtgca agacctattc gcattttaag agctcctaat
    20641 tcaaccaatc ttcctttgcc aaaagctgga ggatacggag aagaggtttt agaaaattcg
    20701 gcttattagt caaatatttg tgtaagaaag gaagtaacag taatttcttt catcacccca
    20761 ctgagtaacc aatagcccag tgggtttttt gggttacaga aatcagttct aaaaaagaat
    20821 cactggtaac ttaaggttgt ctttgataca atggtaacag tgtatttaaa agaaagagag
    20881 gacaacatgg aaaaccataa aaatgaattc accttttcca acaaatcaat tattgcctat
    20941 gtttggcgtg gtgttgtggt tggtattatt gctggtgtta ttgttagttt attccggttg
    21001 ttaattgaag tgacagctga ctgggtgatt gaatggtacc gatacgctca tatcaattcg
    21061 ctgttgctac ttcctatact aagtgtcagt ttgttagctg ttctttttgt aggtttttta
    21121 gtaaagtctg attctgatat aaaaggatca gggatccccc atgtcgaagg agaactaaag
    21181 gggctaatgt ccccagattg gtggagtgtc ctttggaaaa aatttttagg aggaatcatg
    21241 gctatttcta tgggatttat gctcggtcgt gagggtcctt cgattcagtt aggtgctatg
    21301 tcagccaaag gattagctaa gtttttaaaa tcgagtcgtt tggaaaagcg agtgctaatt
    21361 gccagcggag cagcagcagg gctatcagca gcttttaatg ctccaatagc aggcctcttg
    21421 tttgttgttg aagaaattta ccatcatttt tcgcgtttaa tttggattac agcgctagtg
    21481 gctagcttag tggctaattt tatttctctg aatatttttg gattgaaacc agtcttagca
    21541 atgtcagagg cgatgccttt tttaggactc aatcaatatt ggttgttgct tttacttgga
    21601 ttatttctag gctgcttagg ctatttatat gagatagtta tccttaattt taataaactt
    21661 tatgttattt tagggagttg gttacatctg ccagattatt tttacggtat tattatggtt
    21721 tttctaattt taccgattgg ctattattta ccacagttac ttggaggagg acatggttta
    21781 atactttctt tatccaatca acaattgcct ttgatgacta tcttctttta ttttattatt
    21841 cgatttattg ttagtatgtt tagttatgga agtggcttac ctggaggtat ttttttacct
    21901 attttaactt taggtgcttt ggcaggccta ttatttgggc aaatagcttc tcaattaggt
    21961 ttattgaacc aatcttttct ttctcttttt ttgatattag ggatggcagg gtattttgcg
    22021 gcaatttcaa aagcaccact gacaggcatg attttggtaa ctgaaatggt aggtgatttg
    22081 aaacccttga tggccatagc tgtagttact tttgtctcct atttagtgat ggatttgtta
    22141 aatggacagc caatttacga agctatgcta gataagatgg caatgaagca tccaactaat
    22201 cttgttgaac caacattaat tgaattgact gttggtgata agattgcagg gaaatatgtt
    22261 aaagagttaa agcttccaga aaatgtctta attacaaccc aaattcacca tcaaaaatct
    22321 caagttgtgt caggtaacac gcgcctttta tcaggagcaa ctatcttttt ggtcgttaat
    22381 gaagctgata caggttttgt ccgtgaggta ttgatgtagg tattaattta tcacaattcg
    22441 gaaagttcga gcttgtcttt tagactaagt cttcgaacgt tttactgcta gagtttcatc
    22501 tgaaccaaag tgaaacgctt ttgatgaagt caagtaggct ttttgttata ctaacaataa
    22561 agataagcta aggaggctga gatgttagaa attgtttcag aagagctacg tcactatcaa
    22621 gaacaaaaat tgattgagta ccgtaacaaa aatcagttag ccccaaaagg aggcattgta
    22681 tttgcaggtg attctctcat tgaatttttt cctttaaaaa aagcttttgg ttcatgtttg
    22741 cctattatta atcgaggtat tgctgggata gatagtcagt ggttattgcg ccatttttct
    22801 gtccaaatta ctgatttaga gcctaaacat atttttttat taattggttg taatgatatc
    22861 gggcttggat atgataaatg tcatattgtc aagactattg tagagcttat tagccagatt
    22921 cgtagtcatt gtgtctatag tcagatttat ctcttatctt tattaccagt ttccaacaat
    22981 ccccgatatc agaagactgt caaaataaga acaaatgcta tgattgatgc tattaataaa
    23041 gatttagcaa tgattccaac tgtagaattt attaatctta atacttgtct taaagacgaa
    23101 aaaggtggtt tatcagatga gaatactcta gatggtttac atcttaattt tccagcttat
    23161 gctaaactag ctgaaattat taaatcttat atctagcaaa taaatgtact aatagcgatg
    23221 aggcaagaag tcagatagca gatggttcat caatcaatta agaaaaaagt tatcaagata
    23281 ccattaaaaa tagttgctca aagttctttc aaatcaaaag attatgatat cgctatgttg
    23341 agttttagtg tataatgata attaaaggag ttaccatgaa atttgaaaaa aaacaagtct
    23401 tctatttagt ccttacgttt atcttatgct atgggatcct agcaaactgg cgaaatggaa
    23461 cggctatcgt cacgactatt tacaaaacaa gccttccttt tttttatgga gcagcaggtg
    23521 cctatattgt taatattgta atgagcgctt acgaaaaggt atatgtttat atctttaaag
    23581 actggtcaca tgtcttaaaa gtaaaacggg gtatttgttt actgttagcg tatttgacat
    23641 tttttatttt aattacttgg attatttcca ttgtcattcc agacttaatt acaagtatta
    23701 gtactctaac aaaatttgat actataacga tacaagaagt tgttaacaat cttgagcaca
    23761 ataaactgtt agcacgtact attcagtata ttggtggcga tggcaaactt acagagacga
    23821 ttgctaacta tagtcaacag ttattaaagc aatttcttac tgttttgaca aatattttaa
    23881 catctgtcac ggttattgct tctgcaatta ttaatctttt tattagtttt gttttttcgt
    23941 tatacgtttt agcaagtaaa gaagatcttt gtcgtcaggg aaatacttta gtagatactt
    24001 atactggtaa gtacgctaaa cgcattcatt acttgttaga gttgttgcat cagcgttttc
    24061 acggattttt tgttagtcag accttagaag ctatgatttt aggttcgctg actgctagtg
    24121 gcatgtttat attaagatta ccatttgcag ggactattgg cgttttagta gcttttacag
    24181 cattgattcc agttataggt gcttcaattg gagcagctat tggatttatt ttaattatga
    24241 ctcagtccat gtcacaggcc attattttta tcattttttt aattattttg cagcagattg
    24301 aaggcaattt tatttatccg aaagtagttg gtggatcgat tggattaccg gctatgtggg
    24361 tattaatggc aattacaata ggcgcttctt taaagggaat agttggtatg attattgcag
    24421 ttcctttagc agcgacactt tatcaagtga ttaaagataa tattcaaaaa agacaagcta
    24481 ttcaaaaaaa acaagtttcc taaaaggaac ttgttttttt gtcgaataaa aagatggagg
    24541 taatatgata aatgtattct ataaaatgtg atgataataa agccatgcca agagaacgtt
    24601 tgatgcgact aggagcagag tctctaagta atcaagaatt attagcaatt ttattacgaa
    24661 caggtaataa agaaaagcat gtcttagagc tgtcatccta tcttttatcg catttagaca
    24721 gtctggcaga ttttaaaaag atgtctttgc aagaattaca acatttggca ggtataggaa
    24781 aagttaaagc gattgaaatt aaagctatga ttgagttggt ttcccgaatt ttagcgacag
    24841 ataagacatt aactgatagc gtattaacca gtgttcaggt cgctgaaaaa atgatggcag
    24901 ctttaggaga taaaaaacaa gagcatttag tcgtattgta tttagataat caaaatcgta
    24961 ttattgaaga aaaaactatt tttattggga ctgtccgacg ttcacttgca gaaccaagag
    25021 aaattttata ctatgcctgt aaaaatatgg cgactagtct cattgttatt cataatcatc
    25081 cttcaggaaa tattgaacct agttctaacg attattgctt tactgaaaaa ataaaacgat
    25141 catgtgaaga tttaggcatt atctgtctag atcacattat cgttagctat aaagattatt
    25201 atagttttcg agaaaaatca accctttttt aagtcagaag tcattgacga ataagcggaa
    25261 caattgtaaa tcttcatctc gaacaccttg gagaagttca ggatgccact gaacacctaa
    25321 aaaaggaatg gcatcgttgg ttgatatgac tgcttcgatg gtaccatcac gtggatccct
    25381 agcgattact tttaaatcct tagcaacagt ttttaaactt tggcgatgaa aagaattaat
    25441 taatgtctta tgaccataaa taggatataa gatagagtct ggctcaataa tcatttcgtg
    25501 tgataagaaa tcagaaggcg cttcttgcca atgtgagtca atgtgttggt taagatttcc
    25561 tcctaaggca acattcatta actgtgtacc acgacaaatc cctaagattg gtttttttaa
    25621 tgttatagcc tccttgatga tagctagctc aaatgtgtct cgctctggtg aaaaatcatc
    25681 atcaaaagca gccttctctt cttgataata ttttgggtca acgttttgcc caccaattaa
    25741 aatgatttta tcaaccatag atacataagt tttagcggcg gcttcatcac caatgggtaa
    25801 aagaagcggc agccccccag attgagtgac tgcctgtaca aatccagtag gtgcatatga
    25861 ccatgggaga ttatccaaag ccatatttag tctttgattt gcagttattc caataatcgg
    25921 tttggtcatt atacctcctt aatctagatg attttgattc ataaaataga gtagtgtctg
    25981 gagctcactg gtcaaatcga cagattgaac aataactcct ttaggaactt gtaggtgaac
    26041 aggagcaaaa ctcaagattc ctttgattcc tgcttcaata agttgatcag taacttcttg
    26101 agcatgaatg ctgggaaccg ttaagatagc cgtttcaatg tcagtgttcg caatccgttc
    26161 tttgacagat gagataccat ggactggaat attatcagct gttttagtac caacaagagc
    26221 gttatcatcg gtatcaaaac ccatggcaat ttgcatctta ttacgatcat ggaagcggta
    26281 atgtaataga gcacgcccta tattaccgca tccaactaag ataacgtttg ttgtagagtg
    26341 atcattaagc aagtcagcaa aaaaattcat caatttggta acatcatagc caaacccacg
    26401 acgccccaat tctccaaaat aagaaaagtc acggcgtaca gtagcagagt caattcccat
    26461 tgcatctgca atttgtttag aactggcttt ttcaacttgg tcagcatgga atcgtttaaa
    26521 aatacggtaa taaagtgata agcgctttgc agtcgctttt ggaatagatt tgtcaataac
    26581 cactatttta ctccttgtag atttctcgtt aaatatgtct gtctttattg taacaaaaag
    26641 attgtgaaaa atgcaacatt ttgattgttt ttgtatgagt taaaaaagct aaaattagtt
    26701 ttagcactat ataatagtat tcattaggaa agtaagttat gacagtcaaa atgaacaaca
    26761 tgaattaata gcaatagaag cgaaatcatt tattgacatt gtgatatgac taaaaaaaca
    26821 aaatttaaag ttgattagac gactgttttg ctaaaaattt cacgttctac aaggctgtcc
    26881 atcaagaaat aaaatttatc ttgaaggatt tgattatctt ttgttttaaa aatattaact
    26941 aatctaagac cagactgatc ggtgatagct aatttaaacc ctgtgagttc tttattaata
    27001 gtgattttta aagggaaacc atcccctgaa ttaggtattt tttcgagtaa acgtattaat
    27061 tggtagtgcc ctactttagt ttgactaaat gtagtatctc ttagggtata ttttttaatg
    27121 tttggactga ttgtataaag gtatttacag tctttaagct taatttcttt ttcaaaagcc
    27181 atctgttcct cctaaaatat tttttaatgt ttgagctaat tgattaacat cttcaatgct
    27241 attttgatct gaaaaactga tacgaattga ttcttttaaa cgagaagagt catccccata
    27301 gtacgcagct aaaacatgac ttggattaac agctccagca gtacaagcag aaccagttga
    27361 tactgcaatg cctgctaagt caagttgtgt tagtaaaata gtattttggt agcctaaaaa
    27421 tccaatattg agaacatgag gaagataatg agtaccttga ttaatatagt agggtagtcc
    27481 ttctaggagg ctaatcaagt gatgacgtaa actaataatg tgatcagttg attggtcaag
    27541 acaagtcata gcatcagtca aagcttgtgc cataccaata atacctaaca tattttcagt
    27601 acttgcgcgt cgctttcctt cttgatcacc cccgtgaaga agagggtcga ttggttgtcc
    27661 gttactgtat aaaaagccgc atcctttagg accatgaaat ttgtgagcag aagcagataa
    27721 aaagtcaatc ccaagttcgc taggaataat cttgagtttg ccaacagctt gaacggcatc
    27781 aacatgaaaa gcagcttgat ggtctttgag cagattccca atgtccttaa taggtaacag
    27841 gtcacctgtt tcgttattgg catacattat gcttaccaaa atggtgtcat ctctcaaggc
    27901 ttgttttaag tcagataaat ttatttgtcc attttgacaa ggcaaataag tgacctcaaa
    27961 accaaatcgc tcttcgagat aggccatggt atgaagcaca gagtggtgct caatagtagt
    28021 tgtgatgaga tgtttgccct ttgcttgatg agctaaagca tagcctttta tggccatatt
    28081 attgctttca gttccaccgg acgttactat aatttgctgt tcacttgccc ccaagtttct
    28141 agcgatagct tgtcggcatt cacgaaggat tttattggct cggcgaccgt agaaatgaat
    28201 actagagggg ttaccaaagt tatcttgcat agctgctgtc atcgccctaa tcacattagg
    28261 actaagtggt gtggtggcgg cgttatcaaa gtaagtcatg cgttattttt gcctttaggt
    28321 tgataagaaa aaagcggact taagggtctt ctttcatgga tacgaataat agcttctgcg
    28381 attaaatcac tggcgctaag ataagtaacg ttttctggca cacgattttt agttttaaca
    28441 gaatctgtaa cgataatttc tttgattgga gctgtttcta atacatcagc agctccacca
    28501 gcaaataagc catggctagc gaccgcgtaa gtatcagtag cgcctgagcg ttcaagaatt
    28561 ttagcagctt ctgcaaaggt tttcccagta tttaaaatat catcaattaa aatggctttt
    28621 ttaccagaaa catcaccaat aatataacct tgttcacgtt cagaatcatc ttgtgcatag
    28681 tcgataatag caatcgggga atcaagatat tcagccaagc ttctagcacg tttaattcca
    28741 gaatttttag ggctaacgac aacaacatca gaacctgata agcctaattt actgtaacgt
    28801 tctgcaaaaa gagggactgt aaagaggtta tccactggaa tatcaaaaaa accttgtacc
    28861 tgaacagcat gtaagtcaag cgtcactaca cgatcaatac cagctttagt cagcatatta
    28921 gctactagtt tagcagtaat cggttcgcgg ggcttagcga cacggtcttg acgagagtaa
    28981 ccaaagtatg gcaatacaat attgacggta ttagcacttg cacgtttaca agcatcaatc
    29041 atgatgagta attcccaaag attatcattg acaggaaaac tagtggattg aataatatag
    29101 atatcatctc cacgcactgt ttcttcaata ttgatcataa tttctccatt ggaaaattga
    29161 cgtgaagaca tttttccaag agggattcca gcagctttag caattttttc agcaattgga
    29221 agatttgatg tgagtgagaa cagtttgatt tgcttgtcag catatcgttc agtcatgata
    29281 gatttagctc cttaacttca ataaaattgg gtaaactgtt ttattaatga gtcacattac
    29341 ccaaaagtgt ttaattgaca tccctatttt aacagaaaaa cagaaaaatt tcagtttttc
    29401 tgtcgctttt agccattatt tatcgttgaa cttttttaag gtattaatac accgagctac
    29461 tttgctttta gcatatttga aagtaatgtg atgttcagat aaaaaagaat caaaatcaat
    29521 ctttccttga agagcgtcac ttacttctaa ttctaattca tagtctttgg tattggcata
    29581 taggttatag tctaaggcca atttacctat cggtatcact gtctcgcgac gaacggtagt
    29641 taaattgcca aaagttacca gtgcagatgg tttgatacct tttgaaataa taatatcaag
    29701 agcagttgat tcaggaagat tcccatgttt aatcatatct tttgcttgtt caagaaaaag
    29761 agggacattg tactcacgat tccccacttt ttctggcact tttagggtta attcggcgct
    29821 atttacaaat gttcgaatac gtaatgacat tttgttagct tttaaatcaa atgcttttgt
    29881 atcaatatag taattagttt gtgtaacagg cgtcacatgt ttcatttgag ataaaagacg
    29941 gttatactca ttttttgtaa gtagagtttt atattcgatt tctaggttag tcatgggaaa
    30001 ttccttttaa tttgtgctat aataaattgt tatttcaatt ttatattatt atcctggctt
    30061 tgacaagaaa ggagacaata gatgacctta gattgggaag aatttttaga tccttatatc
    30121 caaacagttg gtgaattaaa aataaaacta cggggtattc gcaagcaata tcgtaagcaa
    30181 aatcgttatt ctccgattga atttgttacg ggacgtgtta agtcaattga aagtattaaa
    30241 gaaaaaatga ttttacgtgg tgttattgaa gaaaatatag cacaagatat tcaagatatt
    30301 gctggactac gtattatggt tcagtttgtt gatgatgtgg aagaagtctt agctctattg
    30361 cgtcaacgac aagacatgac aattgtttat gaacgagatt atattcgcaa tatgaagtcc
    30421 agtggctaca ggtcttatca tgtggtggtt gagtaccctg ttgacaccat tgaaggtcaa
    30481 aaaaaggtct tagcggagat tcagattcgt actttggcaa tgaacttttg ggcgaccatt
    30541 gagcattctt taaattataa atatggtgga gacttcccag aagaaattaa aaagcgtctt
    30601 gaagtgactg ctaaaattgc tttagaatta gacgaagaaa tgcgtaagat tcgagaagat
    30661 attcgggagg ctcaattatt gtttgacccc gtgacacgaa atttaagtga tggtgtagga
    30721 aatagtgatg acacagatga attatacagg taaggtaaaa cgagttgcta ttattgcaaa
    30781 tggtaagtac caaagtaaac gcgtcgcctc caaacttttc tccgtattta aagatgatcc
    30841 tgatttctat ctttcaaaga aaaatccgga tattgtgatt tctattggcg gagatgggat
    30901 gctcttatct gcctttcaca tgtatgaaaa agaattagat aaggtacgtt ttgtaggaat
    30961 ccacaccggt catcttggct tttataccga ttatagggat tttgaagttg ataaattaat
    31021 tgataattta agaaaagaca agggagaaca aatctcttat ccgattttaa aagttgctat
    31081 tactttagat gatggtcgtg tggttaaagc gcgtgctttg aatgaagcga cggttaagcg
    31141 tattgaaaaa acgatggtag cagatgttat tattaaccat gtcaaatttg aaagcttccg
    31201 aggtgatggg atttcagtat cgaccccgac agggagcaca gcctacaata aatctttagg
    31261 tggtgctgtc ttgcatccga cgattgaagc gctgcaattg acggaaattt ccagtcttaa
    31321 taaccgtgtc tttagaacct tgggctcatc aatcattatt cccaaaaaag ataagattga
    31381 gttagtgcca aaacgattag gaatttatac catttccatt gataataaaa cctatcagtt
    31441 aaaaaatgtg acgaaggtgg agtattttat cgacgatgag aaaattcatt ttgtttcctc
    31501 tccgagtcat acgagctttt gggaaagggt caaggatgcc tttattggag agattgactc
    31561 atgagatttg aatttgtagc agacaaacga ataaaagtaa aaaccctttt aaaaagttat
    31621 gatgtctcta aaggactatt ggctaaaatc aaatataagg gcggtaatat attagtgaat
    31681 ggtattgaac aaaatgccat ttatttattg caagtgggag atgttgtcac cattgacatt
    31741 ccaaacgaag agccctttga aaagcttgaa gccatccctt ttgatttgga tattgttcac
    31801 gaggatgatc attttttggt gattaataaa cccattggct ttgccagtat tccaagtgcc
    31861 atccactcca ataccattgc taactttatc aaggcatatt atgtggacaa tcattaccta
    31921 gaccaacaag tgcatatcgt gactcgttta gacagagata cgagtggctt gatgttattt
    31981 gctaagcatg gttatgctca tgcaaggttg gacaagcaac tccaaactcg ctcaatagag
    32041 aaacgttatt ttgctctggt ttctggaaat gggatgttgc cagatgaagg agatattatt
    32101 gctccaattg ggcgttcaaa agatagtatt attaccaggg cagttgaccc tatgggcaaa
    32161 tatgccaaga ctagctataa ggttgtggcg cgctattcag aaaatgtgca tttagtggat
    32221 atcaaattgc atacaggacg tacccatcaa atcagagttc attttgccca tattggtttt
    32281 ccacttttag gggatgacct ttacggaggt cgtttggact taggaataac aaggcaagct
    32341 ttacattgtc attatttgaa tttcaaagat ccatttacag aaagtgattg tagctgtgca
    32401 atacacttga cagatgactt tgatagcgtt atcataggtt tacagaaaaa agatagaggt
    32461 tattaaagaa tgagtattcg tagtttattc ggtggtttaa gagaaaaaat tcttggtaaa
    32521 aacatgaaga ttgttttccc agaaggtaat gatgagcgcg tcgtccgtgc agctgctcgt
    32581 ttgaaatttg aaggactctt agaaccgatt atccttggac agtcagaaga ggtacgcaac
    32641 cttttgacaa aattaggttt cgctgatcaa gactatacta tcattaatcc aaatgaatat
    32701 gcggattttg acaaaatgaa agaagctttt gttgaggtcc gtaaaggaaa agcaacgctt
    32761 gaagatgcag ataaaatgct tcgtgatgtt aattactttg gtgttatgtt ggttaaaatg
    32821 ggattagcag atggaatggt atcaggggct attcattcaa cagctgacac tgttcgtcca
    32881 gctcttcaaa tcatcaaaac taaaccaggt atctcaagaa cttcaggtgt cttcttgatg
    32941 aaccgcgaaa atacgagcga gcgttacgtg tttgctgatt gtgctatcaa tatcgaccca
    33001 acagcacaag aattggcaga aatcgctgtt aacacagctg aaacagctaa aatctttgat
    33061 attgatccta aaattgctat gttaagcttc tcaacaaaag gttctggtaa agcgcctcaa
    33121 gttgataagg taagagaagc aacagaaatt gctacagggt tgaaccctga tttggcactt
    33181 gatggcgagt tgcaatttga tgcagccttt gttcctgaaa cagcggctat caaagcacca
    33241 gattcagcgg ttgctggtca agctaatact ttcgttttcc ctgacttgca gtctggaaat
    33301 attggttaca aaattgcaca acgtttagga atgtttgatg ctattggacc aatcttacaa
    33361 ggattgaaca aacctgttaa tgatttatct cgtggctcaa gcgctgaaga tatttacaaa
    33421 ctagccatta ttacagcagc acaagctatt gaaagccaag ggtaactcaa gaccccttga
    33481 tataggattc taactgctaa gtgatgtgtt agaatttgtc ctaagactaa ttgacattat
    33541 taaaaacgag ttggccagac catctcgttt tttttgaagg tgaggaaatg tatgggaaat
    33601 attgccttag taacaggggc ttcagccggt tttggcagag ccattgtcga acacttaatt
    33661 gcagatggga taaaggttat tggtgcttct cgtcgtcttg agaaactcaa ggccttagaa
    33721 acgagctttg ggtcggacaa cttttaccct ttgcagatgg atgtcacaga taccaaagcg
    33781 attgatgatg ctttgacaag tctaccaaat cagtggcaag atattactat tttagtgaat
    33841 aatgcaggct tggcattagg acttgataag gcttatgaag ccgattttga gaattggatg
    33901 acgatgatta ataccaatat tgttgggttg atttatctca cacgtcaatt attaccacat
    33961 atggtctcca aagatgatgg tattattatt aatttagggt caaccgcagg gacaattccc
    34021 tatccgggag ctaatattta tggggctagc aaggcttttg ttaagcagtt ctcactcaat
    34081 ttacgagctg atttagcagg tagccatata cgtgtgacca atattgaacc cggtctatgt
    34141 gaagggacag aattttcaaa gggacagaat tttcaaaggt ccgctttaat ggtgatgagg
    34201 aacgggtgaa ggccatttat caaggagcac atgccattca acctcaaaat attgcgtcga
    34261 ccgtatcatg ggtgattcat cagccccctc atgttaatgt taatcgtatt gagctgatgc
    34321 cagtgtcgca aagttatgga ccgcaaccag ttactcgtga ctagcaaggg atgagtccat
    34381 aagaataata atctttagaa aaatggttta ctgggatggc ttattcttta taagtgagac
    34441 ttaccgattc agtcatcaaa aaaagcagtc aagaagaagg ttttgaggtc ttcaagcgtt
    34501 catgattgct tcttgtgcgt tgatgattta ggctgaactc cctatttaaa atcaagttta
    34561 gtgatctaaa atggaagggc ctgatgtgac atcggaccag ccaacaatgg cttgtccagt
    34621 ttcttctaaa tactggttta ataacggttt aatgtcttga acggtatcca aaactcccac
    34681 cagctgacct tgaggatttt taagagcttg ataatgctgc atgtaaatat gatccaatga
    34741 acccgaagga agtaggaagg ttttacaagg gttatctgat aaacgcaatt cagcaagaat
    34801 ccaatcttct atttctttat cagtagttgg caaaaaactt ccattaaaag tatgattatc
    34861 gactatgatg tttccatttg catcagataa ccttgcttgc aaaggaaggt actgtaaaaa
    34921 atgtccatca aatttttggt aaaatccagt gaggtattgg cttaacgata agactgctgt
    34981 tgcttgatca aagacacttt ctgtcactaa aaagccctga ccatgttgag aagttttgaa
    35041 atagagtgta ggcaagtcaa tgtcatgctc atggaaactg aaaattcctt ggaaagtcga
    35101 caattgattt gactgaatgt tggctttgct agtgagaata gtcacctcaa aaaggtgtga
    35161 gaagtggtgg gaaagctctc taataaacgt ctcttcaagt tggttttcag gtgttaagag
    35221 cgctaaaatg gaaatagcat ttttcataat tgtatgatag ctgtattttc tagaaaagac
    35281 aattaatctg cttatctatc atcataggca agtctaggat ttttgataag gtaattttgg
    35341 agagataggc tgacccagtg acctgttttt ctataagggg gtaatagtct cattatttga
    35401 cttatagtgg gaaacttttt ataatggtca acaacaaacg acaagaaaat aatgagagaa
    35461 aaagtttttg agaattggta gtttctacct tactgaggat acctaagagt ccctttaatt
    35521 agtagatgcg tttgtctaaa aatggtaggg catgcagtga ttccttgtag ttactcagaa
    35581 cgttttgaga aaaaatgaca gagagacagg ttgaatgaac tcatcagctt ttatgctgtt
    35641 actttaacca atgaattgga gaaataatga tacgttttaa taatgtttct aaaacttttg
    35701 gtcagacaaa ggttttgcaa gagcagacct ttcaaattaa tgatcgtgaa ttttttgtgc
    35761 tggttggccc cagcggatcg ggcaagacca cacttttaaa aatgattaat tgccttattg
    35821 aaccaagctc tggagatatt ttactaaata acgtcccaca gacagagtta gatttacgtg
    35881 agatgaggct atctattgga tacgtattgc agcaaattgc tctgttcccc aatctcacag
    35941 tagctgaaaa tattgcgatt atccctgaga tgaagcaatg gtcagctgaa gaaattagac
    36001 aaaaaactga agaattattg gataaggtgg gcctacctgc taaagattat cttgatcgtt
    36061 acccaagtga tttatcggga ggtgaacagc aacgtattgg tattgtacgt gctatcattt
    36121 ctcatcctaa aattctattg atggatgaac cattttcagc cttagatcct atttcacgta
    36181 aacaattgca agagttaatg ttgagtcttc acaaggaatt tgacatgacg attgtatttg
    36241 tgacacatga tattgatgag gccattaaat taggagaccg cgtagctatt ttaaatgagg
    36301 gagaaattgt gcaactggat cgcccagaaa tgattaagac ccaccctgct aatgcttttg
    36361 tggtcaattt atttggaggt gatgagcatg cctagtttgt ttgtaacttt ccaaaaccgt
    36421 tttaatgaat ggctggcggc tctgggggaa cacctgcaaa tttccctttt atctcttatg
    36481 atcgccttgc taataggtgt gcctttagca gcccttctca gtcgcagcaa acgttggtca
    36541 gacattatgt tacaggtaac aggtgttttt caaaccattc cctcactggc tttgcttggt
    36601 cttttcatcc ctttaatggg aattggaacg ttgcctgcag tgacagcttt agttatctat
    36661 gcgatttttc cgattttaca aaacaccatc acaggattaa atggtattga cccaagtctc
    36721 gtggaagcag gaatagcttt tgggatgacc aaatgggagc gattgaaaac atttgagatt
    36781 ccaattgcca tgcctgttat tatgtcaggt gtgcggacgt cagcagtcat gattatcggc
    36841 acagctactt tagcttcctt gataggagcc ggtggacttg gctctttcat cttattaggg
    36901 attgatcgta ataatgctaa cctgattctg ataggggcta tttcttcagc tctgctagct
    36961 attattttca atagtttgtt acagtacctt gagaaagctt ccttgcgacg gattatgatt
    37021 agttttggaa ttaccttact tgcactgcta gcatcgtata ctcctatggc gcttagtcag
    37081 ttttcaaaag gaaaagatac agtggttatt gccggtaaat tgggagcaga gcccgatatt
    37141 ctgattaatc tctataagga attaattgaa gaccaatcag atataagtgt tgagttgaaa
    37201 tctaattttg ggaaaactag cttcttatat gaagccctta aatctggaga tattgatatg
    37261 tatcctgaat ttacaggaac cataacatca agtcttttac gcgacaaacc acctttgtct
    37321 aatgacccta agcaggtcta tgaggatgct aaaaaaggca ttgctaagca agataaactg
    37381 acccttctca agccatttgc ttaccaaaat acgtatgctg ttgctatgcc agaaaaattg
    37441 gctaaggaat atcagattga aaccatttct gatttaaaag cgcatgctga tactttaaag
    37501 gccggtttta ctttggaatt taaggacaga gcagatggct ataagggaat gcaatctcaa
    37561 tatggattac agctatctgt ggcgacgatg gagccagctc ttcgttatca agcaattcaa
    37621 tcaggagata tccaagtaac agatgcttac tctacggatg ctgaaattac gaaataccat
    37681 ttgaaagttt taaaggatga taaacagttg tttccacctt atcagggagc tcctttgatg
    37741 aaaacttcct tattaacaaa acatccagaa ttaaaaggca ttcttaatca attagcggga
    37801 aaaattactg aaaaagagat gcaggacatg aactacgagg tatctgttaa aggagcagat
    37861 gctaataagg tcgctcgaga ttatctgttg aaaacaggct taatccagaa ataaacagaa
    37921 atgttggtcc gtaggactgg cattttttcg tctcttaagt tcaagaagta acaattcata
    37981 aacacaaaaa ccgaacttta aaattaaaaa tagcatataa atttgacaaa acataaacaa
    38041 ataagtaatc tataaaatga aaggtaagtt tttacctaaa gatacctcta atcatcagag
    38101 gtcataggaa gattattttg tatagaggac ttagagaaga aggtgagaac taacttgttt
    38161 ttcttaatct atgttaaaag aggagaaact tatgtttaat gacatccctg tatttgatta
    38221 tgaagatatt caactgattc ctaacaagtg cattattact agtcgttcac aagcagatac
    38281 aagtgtgaca ctcggaaaat accagttcaa gctaccagtt atcccagcaa atatgcaaac
    38341 cattattgat gaaacaattg ctgagcagtt ggcaaaagag ggttattttt atattatgca
    38401 tcgttttgat gaagatagcc gcaaaccttt tatcaaacgc atgcatgaac aaggcttgat
    38461 tgcatcaatc tctgttgggg ttaaagcttg tgaatatgaa tttgtgacct ctttgaaaga
    38521 ggatgctcct gagtttatca ccattgatat tgctcatggt catgctaaca gtgttattga
    38581 tatgattaag catatcaaga ctgaattacc agaaacattt gtcattgctg gaaatgttgg
    38641 tacgccagaa gctgtacgag aactcgaaaa tgcaggtgct gatgctacta aagtaggcat
    38701 cggtccaggc aaagtatgta tcactaaagt taaaaccggt ttcggtacag gtggttggca
    38761 gctagcagca ctgcgctggt gcgcaaaggc agctcgtaaa cctatcattg ctgatggtgg
    38821 gattcgcaca catggtgata ttgctaaatc catccgcttt ggagctagta tggtcatgat
    38881 tggatcgctt tttgcaggac attttgaaag tcctggaaaa acggtagaag tggatggtga
    38941 aacgtttaaa gaatattatg gctcggcgtc agaatatcaa aaaggcgagc ataaaaatgt
    39001 agaaggcaaa aaaatcttat tgcctacaaa aggtcactta tcagataccc tgaccgaaat
    39061 gcaacaggat ttgcaatcgt ccatttctta cgcgggtggt aaagatttag acagtttgcg
    39121 tcatgttgat tatgttatcg tcaaaaattc tatttggaat ggtgattcca tttaaatgat
    39181 atggagttag ctaggaccag taaagaattt tttagcttaa tgctggagat gcttttatca
    39241 tatctctgta ggtattgaaa aaaataagaa acatggtaaa atagtaaatt attataatga
    39301 attcaataat gacatactta tttatgctgt gaattggcgc agcgtctcta caagacacct
    39361 taatgtctaa caataagtaa gcttttaggc ttgcttggtt tttaaccttg caaagtgaga
    39421 aactcttata gtttctggct ttgcggcagg ctttttctat ttgaatcaaa aggagattta
    39481 tgcagttact tgaagaacgc atcttaacag atggcaatat tctaggagag aatattctaa
    39541 aggtagataa ttttttaact catcaagttg attaccggtt gatgaaagca attggtaaag
    39601 tgtttgctca aaaatatgct gaggctggca ttacaaaagt ggttacaatc gaagcttcag
    39661 gtattgcacc agccgtatac gctgcagaag caatggatgt tcctatgatt tttgcgaaaa
    39721 aacataaaaa cattaccatg acagaaggca ttttgacagc agaagtttat tctttcacta
    39781 aacaagtgac gagcacggtg tctatcgctg gtaaattcct atctaaagaa gacaaggttt
    39841 tgattattga tgacttttta gctaatggtc aggcagccaa aggcttgatt gagattattg
    39901 gtcaagcagg ggcacaagtc gtcggcgttg gtattgtgat tgagaaatct ttccaagatg
    39961 gtcgtcgatt gattgaagat atgggcattg aagtgacctc attagcccgt attaaaaact
    40021 ttgaaaatgg taatctaaac ttcttggagg ctgacgcata atgacacatt caacaaagca
    40081 ggaacattct cactcgcaat cagctgtcct tggcttacag catgtgcttt cgatgtatgc
    40141 cggatctatt ttagtaccaa ttatgatcgc aggtgcttta ggttattccg ctagagagtt
    40201 gacctacctg atttcgacag atattttcat gtgtggtgtg gctactttct tacagttaaa
    40261 gttaaccaag catacgggag tgggattacc agttgtttta ggatgtgctt ttcaatcagt
    40321 ggcacctcta tccattattg gagcgcaaca aggttcaggt gccatgtttg gagccctcat
    40381 tgcgtcagga atttatgtca ttttagtggc aggtattttt tctaagattg ctcgtttctt
    40441 cccaccaatt gttacaggat cggtcattac cgttattggc ttaagtctcg taggtgttgc
    40501 tatggggaat atgggagata acgtcaaaga gcctacggca caaagcatga tgttatcatt
    40561 gttgacgatt gtcattattc ttttagtcca aaaatttacc aaaggttttg tgaaatccat
    40621 ttccattttg attgggcttg tagcgggaac tttggtttca gctatgatgg gattagtaga
    40681 tacaactcct gttgtagagg cttcttggat tcatgtgcca accccatttt attttggcat
    40741 gccaactttt gaaatcactt ctattgtgat gatgtgtatc atcgcaacgg tttctatggt
    40801 tgaatcaaca ggtgtctact tagcgctttc agatttgact aatgatcaat tagatgaaaa
    40861 acgtttgcgc aatggttatc gctcagaagg gatcgcagtc tttctcggtg ggttatttaa
    40921 caccttccct tacactggtt tttctcaaaa cgttggactt gtccaaatct caggtattaa
    40981 aacccgtcgt ccgatttact acgcagcagg tatccttgtg gtcattggcc tactccctaa
    41041 atttggagca atggcacaaa tgattccaag cccagttctt ggtggagcca tgcttgttct
    41101 ctttggtatg gttgctcttc aaggaatgca aatgcttaat cgtgttgatt tccaaaagaa
    41161 cgagtataat tttattattg ctgccgtttc catctcagca ggtttaggct ttaatggcac
    41221 taatcttttt gccagcttac cagagacagc tcaaatgttc ttaacgaatg gtattgtgat
    41281 tgccactttg acctctgttg tcttaaattt agttttaaat ggcaaagaca aacaagatga
    41341 ataattaaaa aacggctaat tgagccgttt tttctgtgat cagttttgag caaaccgtta
    41401 acccctttta atgatagtat ggttgaaaat agtgtctcaa accatttgac ataaggacat
    41461 ggtgctttct tgttactaga agaccttcaa tattatcaaa ggtatttaaa agtgtgataa
    41521 tcattgagct atcaagacca aataaacggg tagtccatat gtcacaataa aaagaagaag
    41581 gagccatgat cgttaagcta agcatatctg tttctatagg gtagcctgtc tgacgatcta
    41641 agatatgatg atactgtttc cctttggatg taaactgcct ttcataaatt ccagaagtca
    41701 caacagagtg attgttaacc ttgatgacac caagatgttg cccacgttta gcatctggtt
    41761 tttgaatgcc aattcgaaaa gtcttgtcag ggcttttcgg attaggtcca tgaacgcgta
    41821 catttccacc taaattaata agggcagagt caataccatc ttcaatcagg taggccataa
    41881 tcttatcagt aatataacct tttgctaagg ctcctaagtc taaagccatt cctacttgtt
    41941 gtaaaaagac agtctgtttt ttgtcatcta taagaacctg tctaggatct gttagagcta
    42001 actgttgtga gataaggtta tgactaggca ctctagcatc ttcaaatcca attcgccagg
    42061 cttgaacaag tggcccaatg gcgatattaa gattacttgg tgttgacaag ctatgagctt
    42121 taccaatttg gattaggtta aataaatcag agtgaacgct gacgggttta acaccagccg
    42181 cctgattaat agccatcaat tcagagtttg aatcattagc tgaaaaacga tttttgtaag
    42241 tatataataa gtcaatgact ctacttagtt gttggcaagc tttgtcagat tcgatctgaa
    42301 tatcaataac tgttcccatt agctttaatt gttgcgtaac aaacataatt agtcctcttt
    42361 ttagcagaat acccccacta aggagtgggg gaaggtcatt atgatttttg tttcatttca
    42421 ccttgaggat agtaagttcc ttcaggcata tcgttgataa aaacatggat attttcttta
    42481 ggtgcttttg caatgcgtga gactacttcg gtaacctcgc gagctaattg atttttttgt
    42541 tcttgtgagc gtccctcaaa caagtcaatt gttacaaatg gcataatgtt actcctttat
    42601 gttattgggc atcattgtaa cataatgctg tgaaaaagta tacactgttt tcaatggctg
    42661 ggaaaacttc agcctttgtg gtaaaatagg tagaatacta tagaaagtag gattttccgc
    42721 tttggctcaa ctctattata aatatgggac aatgaattct ggtaaaacca ttgaaatttt
    42781 aaaagttgct cataactatg aagaacaagg caaacctgtt gtcattatga caagtgctct
    42841 tgatacacgt gatggttttg gcatcgtctc aagccgtatt ggtatgcgac gagaagctat
    42901 ccccatttca aatgacatgg atattttcac atttattgcc caattggagg aaaaacctta
    42961 ttgtgtcctg attgacgaaa gccaattttt aagcaagcaa aatgtttatg atttggcgcg
    43021 tgtggtggat gagctaaatg ttcctgtcat ggcttttggt ttgaaaaatg atttccaaaa
    43081 caatcttttt gaaggttcaa aacatttgct attattggcc gataagattg atgaaatcaa
    43141 aacaatttgc caatactgta gtaagaaagc aaccatggtt ttaaggacag aaaacggaaa
    43201 acctgtctat gaaggtgatc agatacaaat tggtggtaat gaaacatata tcccagtttg
    43261 tcgaaaacat tattttaatc cagatattta aaaggtaatc ggttttaaag gagatacaaa
    43321 gaaaaatgaa tatttatgat caattgcagg cagttgaaga tcgttatgaa gagttaggtg
    43381 agttacttag tgatccagat gttgtctcag atacaaagcg atttatggaa ttatcacgtg
    43441 aagaagctaa tactcgagaa actgtcacag cttatagaga atataagcaa gtcattcaaa
    43501 ccatttcaga tgctgaagaa atgatcaaag atgccagtgg tgatcctgaa ttagaagaaa
    43561 tggctaaaga agaacttaaa gaatcaaaag cagctaaaga agagtatgaa gaaaaattaa
    43621 aaatccttct attacctaaa gatcctaacg atgataaaaa cattatttta gaaattcgtg
    43681 gtgctgctgg tggcgatgaa gcagcgcttt ttgctggtga tcttttaaca atgtatcaaa
    43741 aatatgctga aactcaagga tggcgttttg aagttatgga atcatccgtt aatggtgttg
    43801 gtggtattaa agaagtggtt gccatggttt ctggtcagtc agtttattct aagcttaaat
    43861 atgaatcagg agctcatcgt gttcaacgtg tcccagtgac agagagtcag ggtcgtgttc
    43921 atacttcaac tgcaactgtt ttggtaatgc cagaagttga agaagtagaa tacgacattg
    43981 atcctaaaga ccttcgcgtc gatatttacc acgcgtctgg tgctggtggt cagaatgtca
    44041 ataaagttgc tactgcagtt cgtatggtac acattcctac aggaatcaaa gttgaaatgc
    44101 aagaagagcg tactcagcaa aaaaaccgtg ataaagcgat gaaaatcatt cgtgctcgtg
    44161 ttgctgacca ttttgcgcaa attgctcaag atgaacaaga tgctgaacgt aaatcaacag
    44221 ttggtactgg tgatcgttca gaacgtattc gtacttataa tttcccgcaa aatcgtgtaa
    44281 cagatcatcg tattggtttg acacttcaaa aattagatac tattttatct ggaaaaatgg
    44341 atgaagttat tgatgcactc gttatgtatg atcagacaaa gaaattagaa tctctcaata
    44401 actaatgaac tatgctacat taatcagaac ttatgaagat aaactagaac aaatagatga
    44461 ggatcgcgaa aatctcgcct atgtttttag agaaatcaaa gaatggtcaa gtctagatat
    44521 gttgattcac caaaatcaag ctgtgactcc tgaagatgct gttcttttgg aacatatttt
    44581 ctgttctcta agtcagcatt tatctcctca atatattaca ggtaatgctt attttcgtga
    44641 tttgaagctt gcagtcgaca agcgtgtatt aattccaagg ccagaaacag aagaattagt
    44701 tgacatgatt ttagctgaaa atcttgatgc accgttgaat gttttagata ttggtacagg
    44761 tagtggagcg attgctattt ctctcaaaaa agaaaggcct aactggcaag taacggcatc
    44821 tgatatttct agagctgctc ttgacttggc aaaagccaat gctgatgctt accagcttga
    44881 tataacgttt attgaatcag atgtttttag tctcatttca gaaacatttg atattattgt
    44941 gtcaaatcct ccctacatat cctacgagga taaggaagaa gtcagtctaa atgttttgca
    45001 atcagagcct catttggcac tttttgctaa agaaaatggc tatgctattt accgtaagat
    45061 tattgaacaa gctgacaatt acttaactaa ggaggggaag ctttactttg aaattggtta
    45121 taagcaagct gaaggtataa aagatatgtt acaagcctat tttcctcaaa gacatatacg
    45181 agccgtaacg gatatatttg gcaaagaaag gatggtggtt gttgattatg gagtatctag
    45241 ctagtatcat tgaatcaggt gatgcccttg ttttaccaac agaaacggtt tatggtcttt
    45301 ttgccaaggc tttagatgaa aaagcagtaa atgctgttta tgacttaaaa caaagacccc
    45361 gagataaagc aatgaacctt aatgtggctg attttaatag tatcttagcc ttttcaaaag
    45421 aacagccgag atacctcaag aaattatacc aagccttttt accaggtccg ttaacgatta
    45481 ttttaaaagc taatgaccag gttccttatt ggattaattc gggactttca acggttggtt
    45541 ttcgcttacc cagtcatcca attaccgcag ctttaattca aaaaacaggc cctttaattg
    45601 gtccttctgc aaacttatct ggcaaagcta gtggtcgagt ttttgatcat attatgcaag
    45661 attttgactt tcaagtgttt ggttatgcag atgatccttt tctaacaggt aaggattcaa
    45721 ccatcttaga cttatcaggt gagcgtgctg tgattttacg tcaaggtgct atcaccaaag
    45781 aagaattatt agctaacgtg cctgaacttc gcttttaaga gaggagccgc ttggaatgtt
    45841 gagaccatta agtaaaacgg actgtcctat gctacaagaa attaatgcca aagcccttgg
    45901 ctatttagtt tcgcttgact tgttagaaag gcaatatgaa cgattgattg aggattgcca
    45961 tcactatttt ttagcttatg ccgacaagga taccaatcaa ttattaggct atgttcacgc
    46021 tgaacggtat gaaacccttt atgcttctga tggtcttaat ctcttgggct tggctgtttt
    46081 accagcctat cagagaaggg gaattgggag tgccttacta agggccctgg aatcccaagc
    46141 taggcaggaa ggcattgcct ttattcgcct caattctgcc agtcatcgta aagaagcgca
    46201 tgctttttac cgaaacctcg actatgcaga tgataaaaca caattacgat tcatcaaaaa
    46261 cttacaagga gatgacaatg atttttgata aaggtaatgt tgaagacttt gataaagagc
    46321 tttgggatgc cattcatgct gaagaagaaa gacaagaaca tcatatcgaa ttgattgcat
    46381 ctgaaaacat ggtttcaaaa gctgtcatgg cagctcaagg ttctgtttta accaataagt
    46441 atgcagaagg ctatccaggt aatcgctatt atggtggaac agaatgtgtg gatattgtcg
    46501 aaacgcttgc tattgaacgt gctaagaaac ttttcggtgc cgcatttgcc aatgttcaag
    46561 cccattccgg aagtcaagcc aatgcagcag cttatatggc cttgattgag gctggtgaca
    46621 ccgtattagg aatggatttg gctgcaggtg gtcatctcac gcatggctct ccagtaaatt
    46681 tttctggaaa aacctaccat tttgtgggtt actcagtaga tactgatact gaaatgttaa
    46741 attatgaggc gattttagaa caagcaaagg cggtccaacc aaagcttatc gttgcagggg
    46801 cttcggccta ctctagaagc attgattttg aaaagtttcg tgccattgct gatcacgtag
    46861 gtgcttactt aatggtggac atggctcaca tcgctggcct tgtagcagca ggtgtgcacc
    46921 cgagtcctgt cccttatgcg catattgtga catcaacaac ccataaaacc ttacgtggtc
    46981 ctcgtggagg cttgatatta accaatgacg aagctcttgc taaaaagatt aattcggccg
    47041 ttttccctgg tttacaagga ggacctctgg aacacgtgat tgctgccaaa gcggttgcct
    47101 tcaaagaagc cttggatcca gcctttaaag actatgcaca agctattatt gataatactg
    47161 cggctatggc agcagttttt gcacaagatg atcgtttccg tctgatctca ggtggtacag
    47221 ataatcatgt ctttttagta gatgtcacta aggttattgc taatggtaaa ctagcgcaaa
    47281 atcttctgga tgaggttaat attaccctta ataaaaatgc cattcctttt gaaaccttgt
    47341 cgccatttaa aacctcaggt attcgcattg gttgtgctgc cataaccagc cgtggtatgg
    47401 gtgttaaaga gagtcagacc attgcccgtc ttatcatcaa agccttggtt aatcatgatc
    47461 aagaaactat tcttgaagaa gtccgtcagg aagtgcgtca gttaaccgat gccttcccac
    47521 tctataaaaa gtaaggaatc atgttagata gttatataaa acgtattgtc attcatcaat
    47581 tttctcccaa tgacactgag ttactgttat cagatcgttt agtgagcatt acaccgcgaa
    47641 ttgatgaata tttccgaaaa aaattggcca aagtgttctc ggacgaagcc aaacgtggtc
    47701 agtttgaggc taataatacc ttttttacaa ccattggtga tgatttgttg gaaacgtctg
    47761 ttaccattgc ccagttatgg aaggaagcct ttgttatttc agaggatcaa aaaaccaatg
    47821 acttagtctt tgttcagttt gataaggatg gcgaaccctt ttttgctttc ttgcgaattg
    47881 ctttgaaaga acagtttgct cacctttctg acaattatga acatccattt acggtgacgc
    47941 agaataatct gccgagtccc acccagacac cagatgaagc actagtcatt aatctcaaat
    48001 ctggtcagta ctacttgatt gaaaagcgtg tgaagcataa tggcagtttt gctaattatt
    48061 tttcagaaca cctcttgaaa gttacacctg agcaatctgt taaaaagtct atcaaaatga
    48121 tagaacaaac cgctcaaaaa attgcagagc attttaatca agatgatttt acttttcagt
    48181 caaagatgaa atccactctt tttaagcagt tagaagcaga cgacgtttta tcaccagaaa
    48241 aattagctga ccaactcttt gatgataatt taacagcccg tttaaccttt gtggatcaag
    48301 tcaaagatgt cattccagaa cctataaaaa ttagcgatat tgaacattct agacagataa
    48361 aaaaactaga aaatcaaaag ctatcgttat caaacgggat agaattaaca gtaccaaatg
    48421 ccatttatca ggatgctgaa gcggttgaat ttttgttaaa tgatgatggg acttattcaa
    48481 tcttgattaa aaatatagag gatattaaaa ccaaatagat gtttagactt ttaaagcgtg
    48541 cctgcagttt tcttcttctt tttgtcattt atcaatcctt tgttattcat cacaatgttc
    48601 aacgtgtttt ggcatataag cctatggttg agaaaacatt agcagaaaat gatacaaagg
    48661 caaatgtaga cttagtctta gctatgatat acaccgagac aaaaggagga gaagcagatg
    48721 tcatgcaatc cagtgaaagc agttcaggac aaaaaaactc gattacagat agtcaggcca
    48781 gtattgagca cggggtgaat ttactgtctc ataatcttgc tctagctgag gaagcaggag
    48841 tagattcttg gacggctgtt caggcctata attttggaac agcttatatt gattatattg
    48901 ctgaacatgg aggtcaaaat accgttgatt tagcgactac ctattccaaa accgttgtgg
    48961 cgccaagcct aggcaacact agtggtcaaa cctattttta ttaccacccc ctcgccttga
    49021 tttctggagg taagctttat aagaatggcg gcaatatcta ttactcacga gaagttcatt
    49081 tcaatcttta tttaattgaa ctgatgagcc ttttttagaa agttatcatg aaacgattac
    49141 gtccatatgt gaaagggtac ctaaaagaaa gtatcttagg tcctcttttt aaattattag
    49201 aagctttatt tgaattatta gtccctttgt taattgctaa catgattgat atatcgatta
    49261 gtcaacacaa cagccaggga attttgaggg ttgttttaac attatttggt ttagcaacca
    49321 ttggcttatt gctttccgtt acagcccagt atttttcttc gaaagcagct gttggtttta
    49381 caagacaaat gacagatgac ttgtttaaaa aaatcatgtt tttgagcaag gaggaccaag
    49441 accatcttgg ttatgctagt ctgttatcac gattgaccag tgatagtttt caaattcaaa
    49501 ctggtatcaa tcaatttttg cgtctttttc taagggcccc tattatcgta tgtggtgcta
    49561 tggtaatggc ttattggatt agtccaagtc taaccctatg gtttgtgatg atggtgattg
    49621 tcttactaac acttgttttt gttatgtcac atttattagg tcccctttat ctcttaatcc
    49681 gccgagaaac agatcacttg gttcgtttga ctagccaaca gttacaagga atccgagtaa
    49741 ttaaggcttt taatcaaacc caaaaagaac tccaagcatt taagcagcaa aatatgctct
    49801 taagtcgcca ccaatatcaa gctgctaccc tagccaatgt cttgaatcct atgacttttt
    49861 tagtggtcaa tcttaccctg ttaatcttga tttggcaagg aagttggcaa gtagctcata
    49921 gatcgcttag tcagggcatg ttggttgctt taattaatta cctcttgcag attttggcag
    49981 aacttttgaa aatgaccatg ttgatgggaa ccatcaatca aagtgtaaca gccgcaaaac
    50041 ggattaatca ggtgtttgtt ttagctgatg aagcgccttt gcccttactt aaagatggtc
    50101 ctatttcaac tcatctgttg accatccggc acttgacctt tacttatcca ggagcagcag
    50161 agccaagtct gtatgatatt cagttaagtg ctgatcaggg agaatggatt ggcatcattg
    50221 gtggtactgg agcaggcaaa acgactttga ttgacttgat ctgccaaact tattcacaat
    50281 atagtggtga gattagtctt aattggcaag gagaagtgcc aaaaacacta accgagtggc
    50341 gaaatgtgat tgctcttgtt cctcaaaaag ctcaattatt taaaggaacg attagaagta
    50401 acctcctact gggacaatca atgcctatta gtgatgagga actgtggcgg gctcttgaat
    50461 tggctcaagc aaaagagttt gtcgctgcct taccagaaca attagaggca cctgttgaag
    50521 ccttcggccg tcatttttca ggagggcaac ggcaacgtct agctattgcc agagcacttc
    50581 tgaagccaaa acccattttg attttggatg atgccagttc cgccttagac aatgagactc
    50641 gaggacgact ttttaaagct ctaaaagaag agttatcaga tgttttagtg attctagtca
    50701 cgcaatctat taagaatttg cagtttgctg ataagatttt agtgttggaa cagggccatc
    50761 aattggattt tgccagtcat gaccagttaa aagtaagcaa tgcactttat caagaaatgc
    50821 tagcgcttta cgtgaagggg gagcaaaaat gattaaaaca gatcatcacc tcctaaagcg
    50881 agtgcttcag gatttgttaa aaaaacctct acctgtttgt attctcgtga tcgctagttt
    50941 tgtacaagtt ggtttaagtg tttacttgcc tgttcttatc ggtaaagccg ttgatatgag
    51001 tttgtcagtc aattcttggc aaactttaaa atggctgttg gggcaaatgt tagttattat
    51061 tgtggtcaac acattgattc aatgggtgat gcctcttgtc tatagtcgtt tgctatatca
    51121 atatagccag caattgaaag ataagctttt ggaaaaaatt catcgtcttc cttttgcata
    51181 tctagatcgt caaaccattg gagatttggt tagtcgagtg ataaccgaca ctgagcaact
    51241 gatcaatggt ctacaaatgg tttttaacca gtttatactt ggactattga cgattctgtg
    51301 taccattata gctatggcac agattgactg gctcatgtta atcttggttt tggtgttaac
    51361 accaagctcc ctctttttag ctcgttttat tgctcaaaag agttttcatt atgcacaagc
    51421 acagaccaaa agcagaggaa atttagctca gtttactgaa gaaatccttc gtcaagaagg
    51481 tttggttcaa ctgtttaatg ctcaagagca gtctatctgt gattatcatg ttttaaataa
    51541 aacctattgc gaggcttcac aaaaagccat tttttacgcc tcaacagtca atccagccac
    51601 tcgctttatt aatagtgtca tttatgcttt actggcaggt ttaggggctg ttcgtatcat
    51661 ggctggactt ttttcagtag gtcagctaac cactttttta aatgttgttg tccaatatac
    51721 caaacctttc aacgatattt catctgtctt ggcagagata cagagttctt tagcttgtgc
    51781 gcaaaggctt tatgaccttt tagatattga aataaaagag caagagcact ttctaacatt
    51841 taaggcatca gcggttaagg ggcaaattga ctttgaagaa gtaagttttt cttatcaaaa
    51901 agacaggccc ttactgaaag atattaattt ttctgttcct gcgggttcca aggtagcgat
    51961 tgtagggcct acaggtgcag gtaaatcaac tttaatcaat ttactcatgc gcttttatga
    52021 acttgatgct ggtagcatca agttagataa agttcctatt aagtgttatg ctaaggaaga
    52081 acttaggtcc attactggca tagtattgca agaaacctgg ttgaaagatg cgactgttca
    52141 tgagttgatt gcttacggca gtgaagaggc tagccgtgat gaagtagtgg cagcagccaa
    52201 agcagctcat gcacacttct ttattatgca acttcctaag acttatgata cttacttaag
    52261 tgcttctgat gatgctttgt cccaagggca gctccagtta ttagctattg ccagaatgtt
    52321 tttgaaaaaa ccaaaagtct tggttctaga tgaagccacc tcctctattg atattagaac
    52381 agaagctgtt attcaagagg cactaaaaga actcatgaga ggaaggacca gctttatcat
    52441 tgcccatcgt ttatcaacga ttcaatcagc tgatttgatt cttgttatgg atcaaggtcg
    52501 attggttgag tggggaacac atgccagctt aatgtcaaaa aacggctgtt atgctagatt
    52561 acaaaagata gaataaagtt cgtcttttaa caaaggttaa ctttttaagc cttcctaaag
    52621 ttagttaatt aacaaacttt aagctgttat ataaaaaaat aacagcgaaa aagcggacga
    52681 tcttttttta taagtgagaa aaaacagttg actaaagaag aaataactca aaaaagtgag
    52741 caatatattt ggaagcgttt tttgactgtg gtataataga gccctatata atattgtgaa
    52801 atgaggaaca aaatatatga gtaaaatcgt tgttgttggt gcaaaccatg ctggtacggc
    52861 ctgtatcaag actatgttaa caaactacgg tgatgctaat gagattgttg tatttgacca
    52921 aaactcaaat atttcatttt taggctgtgg tatggcactt tggattggtg agcaaattgc
    52981 tggaccagaa ggacttttct attcagataa agaagaatta gagtctttag gggctaaagt
    53041 ttacatggaa tcacctgttc aatcaatcga ctacgatgcc aaaacagtga cagcgcttgt
    53101 tgatggtaaa aaccacgtgg aaacttacga caagttgatt tttgcaactg gctcacaacc
    53161 tatcttacca ccgattaaag gtgccgaaat caaagaagga tcacttgaat ttgaagcaac
    53221 tcttgaaaat cttcaatttg tgaagttata ccaaaactca gctgatgtga ttgcaaagct
    53281 tgaaaacaaa gacattaaac gtgtagccgt agttggtgct ggttacattg gtgttgagtt
    53341 agctgaagct ttccaacgca aaggcaaaga agtggttctg attgatgtcg tggacacttg
    53401 cttggcaggt tattacgacc gtgatttgac tgacttaatg gctaaaaaca tggaagaaca
    53461 tggtattcaa ctagcctttg gtgaaacagt taaagaagta gctggtaatg gtaaggttga
    53521 aaagatcatt actgacaaaa atgagtacga tgtggatatg gttatcctcg ccgttggttt
    53581 ccgtccaaac acaactcttg gaaatggtaa gattgatctt ttccgtaatg gtgctttcct
    53641 tgtcaataaa cgccaagaaa cttctattcc aggtgtttat gctatcggtg actgtgcaac
    53701 tatctacgat aatgctactc gcgatacaaa ctacattgct ttagcttcaa atgccgtccg
    53761 tacaggaatt gtagcagcac ataacgcttg tggtacagac cttgaaggta ttggcgttca
    53821 aggctcaaac ggtatttcca tttacggatt gcacatggtt tcaactggtt tgacacttga
    53881 aaaagcaaaa cgtcttggtt ttgatgctgc agtgactgag tatactgata accaaaaacc
    53941 tgaatttatc gaacacggta acttcccagt aaccattaag attgtttacg ataaagactc
    54001 acgtcgtatc ttgggtgctc aaatggcagc ccgtgaagat gtgtcaatgg gaattcacat
    54061 gttctcactt gctatccaag aaggcgtaac cattgaaaag ttggcattaa ctgatatttt
    54121 cttcttacca catttcaaca aaccttacaa ctacatcaca atggcagcac ttggtgccaa
    54181 agactagtat ctgataggaa aaagccagag cagttcagca gcactggctt ttttgctttt
    54241 taattgttgc aacctgaaaa gttctaaact tatgattatc ctcttatagc acgttggaaa
    54301 aggaatatca ggcaaaacaa aaagagaccg aagtctcttt tgtgattagt ttttagcagc
    54361 agaagcaaat tcttccttag caaaggcttc atcaatgata gctttcaatt gatttgcaga
    54421 ggcttgcatt ttttgtaatt cagcatcgtt aagtggaatg tttactggac gaacaattcc
    54481 atacgctcca acgatagctg gttgaccaat atagcaatct tcaacacctt cgtattggcc
    54541 ttcttggaaa acagaaagcg gaagaacagc attttcatca tcaagaatag cttttgtaat
    54601 acgagctagg gcaactgcaa taccgtagaa agtagcacct tttttattga tgattgagta
    54661 agcagcatca cgtacagaaa tgaaaagatc aactaggcct tgctcatcaa tgtcacgatt
    54721 agcttggagc cagtcgtaaa gacctacacc agcaacgtta gcgtgtgacc aaacagcaaa
    54781 ttcagagtca ccgtgttctc ccatgatgta agcgtgaacc gaacgcgcat caacaccgat
    54841 tttagcagcc agagcttgac ggaaacgggc tgaatctaga gatgtacctg aaccaatgac
    54901 acgctcttta gggaaacctg agaatttcca tgttgagtaa gtcaaaacgt caactgggtt
    54961 agcagcaaca agaaagattc ctttgaaacc agatgcaacg atttgtgtaa caacctcttt
    55021 attgatacga aggttttttt caacaaggtc aaggcgtgtt tcacctggtt tttgaggagc
    55081 accagctgtt aagacaacaa gatcagcatc atgacagtct gagtagtcag ctgcatagat
    55141 ttttttaggt gatgtaaaag ctagagcgtg gcttaaatct tctgcatctc cttgagtttt
    55201 ttctttaaaa atatcaataa taccaagttc ttgagcaata ttttgtgtta ccaatgcaaa
    55261 agcgtatgat gaaccgacgg caccatcacc gaccaagatt acttttttgt gttgtttagt
    55321 tgcagtcatt tctaaacatc tccttaattt tattagagca aaagctcata cggtcttatt
    55381 ttaacatatt gataattgtt tgtcattgaa ttcagaaaaa taatgaaaat agtttactga
    55441 taacgcttcc aataagaaaa aataaggaat ttatggtata atgaaatcta gtattgacta
    55501 gaaaaggagc attccttaaa tgcaagatcg aaatttaatt gacgtcaatc tgacaagtga
    55561 aatgaaaacg agtttcattg actatgccat gagtgtcatt gtggcaagag ctttgccaga
    55621 tgtgcgtgat ggtctgaaac ctgtgcatcg tcgtattctc tatgggatga atgaactcgg
    55681 tgtgacacct gataaaccgc ataaaaaatc tgctcgtatt acgggtgatg tcatgggtaa
    55741 atatcacccg catggggatt cttctattta tgaagctatg gttcgtatgg ctcagtggtg
    55801 gagttatcgc catatgcttg ttgatgggca tggcaatttt ggttctatgg atggtgatgg
    55861 tgctgccgct caacgttata ctgaagcgcg catgagtaaa attgcgcttg aattattaag
    55921 ggatatcaat aaaaataccg ttaattttca agataactac gatggaagcg aaagagaacc
    55981 agtagtatta ccagctcgct ttcctaattt attagtcaat ggagcgacag ggattgccgt
    56041 tgggatggca actaacattc cgcctcataa tttggcagag tctattgatg ctgtcaagat
    56101 ggtaatggaa catccagact gtacaacaag agagttgatg gaagtgatcc ctggacctga
    56161 ctttccgact ggtgcccttg ttatggggcg ctcaggtatt catagggctt atgatactgg
    56221 taaaggttct atcgttttac gctctcgaac agaaattgaa acaactcaaa caggtcggga
    56281 acgtattgtg gttactgaat ttccctatgg cgtcaataaa acgaaagtgc atgaacacat
    56341 tgttcgttta gcacaagaaa aaagactaga aggtattaca gcagttcgtg acgaatctag
    56401 ccgtgaagga gtgcgttttg ttatcgagat tcgtcgagag gcatccgcga cagttatctt
    56461 aaataacctc tttaaattaa caagtcttca gacgaatttc agttttaata tgttggctat
    56521 tgaaaatggt gtgcctaaaa tattgtcact cagacaaatc attgataatt acattagtca
    56581 tcaaaaagaa gttattatac gtcgcacccg atttgataaa gataaagctg aagccagagc
    56641 tcatatttta gaaggcttac tgattgccct tgatcattta gacgaagtga ttgctattat
    56701 tcgtaacagc gagacagatg tcattgctca gacagaattg atgtcacgtt tcgacttgtc
    56761 tgaacgccaa agccaagcta ttttggatat gcgtttgcgt cgtttgactg gtttggaacg
    56821 tgataagatt caatcagaat atgatgattt gttggccttg attgctgact tatctgatat
    56881 tttagccaaa ccagaacgta ttatcactat catcaaagaa gaaatggatg agatcaagcg
    56941 taaatacgct aacccacgcc gtacagaatt aatggtcggt gaagtccttt ctctagaaga
    57001 tgaagatcta attgaagaag aagatgtttt gattaccttg tcaaataaag gttatatcaa
    57061 gcgtcttgct caagatgaat ttcgtgccca gaagcgtggt ggacgtggcg tccaaggaac
    57121 tggagtcaac aatgacgact ttgtacgtga attgatatca acaagtaccc atgatacttt
    57181 gctcttcttt acgaattttg ggcgtgtcta tcggttgaaa gcttacgaaa ttccagaata
    57241 tggtcgtaca gcaaaaggtc ttccaattgt taatctattg aaattagaag atggtgaaac
    57301 cattcaaact attatcaacg cacgtaagga agaaacagca ggtaaatcct tcttctttac
    57361 aacgaaacaa ggcattgtca aacgtacaga agtttcagaa tttaataata tccgtcaaaa
    57421 cggtctccgt gctcttaagt tgaaagaggg agatcagctg attaacgtcc ttttgacaag
    57481 cggtcaggat gacattatca ttggaactca ttccggatat tctgttcgtt ttaatgaagc
    57541 aagcatccgc aacatgggcc gatcagctac gggtgttcgc ggggttaagc ttcgtgaaga
    57601 tgatcgcgtt gttggcgcgt cacgtattca agataaccaa gaagttcttg taattaccga
    57661 aaatggcttt ggtaagagaa caagtgctac agactatcct acaaaaggta gggggggaaa
    57721 aggaataaaa acagctaata ttacccctaa aaatggtcaa ttggctggat tggttaccgt
    57781 agatggtaca gaagatatca tggtgattac aaataaaggt gtcattattc gcacaaatgt
    57841 tgcaaatatt tcgcaaacag gtcgtgctac tttgggtgtt aaaattatga aactagatgc
    57901 tgatgctaag attgtaacat ttaccttggt tcagcctgaa gacagtagta tcgctgaaat
    57961 caatactgat cgtgaaaata gtatttcaaa aaataaagat aattaatcat aataaggagt
    58021 aaaaaatggt aaaaaaacaa aagcgtcgaa aaataaaatc catgtcttgg gcaagaaaac
    58081 tactaatagc tgtactttta attctaggtt tagcattgct ttttaacaaa cctatccgaa
    58141 atacattaat tgctcgaaac tctaataaat atcaagtaac gaaagtttcg aaaaaacaaa
    58201 tcaaaaagaa taaagaggct aagtcaacat ttgatttcca agccgtagag ccagttagta
    58261 cagagagtgt cttgcaagca caaatggcgg ctcagcaact tcctgttata gggggcattg
    58321 ccataccaga gcttggcatt aatttaccaa tttttaaagg tttaggaaat actgagctta
    58381 tttatggcgc aggaacgatg aaagaagaac aagttatggg aggagaaaat aattattctc
    58441 ttgccagtca tcatattttt ggaattacag gttcatctca aatgctcttt tcgccgcttg
    58501 aaagagcaca aaatgggatg tccatctatt taacagataa agaaaaaatt tacgaataca
    58561 tcataaaaga tgttttcacg gtagctcctg aacgcgttga tgttatcgat gatacagctg
    58621 gtctcaaaga agtgacttta gtgacttgta cagatatcga agcaacagaa cgtattattg
    58681 tcaaaggaga actaaaaaca gaatacgatt ttgataaagc gcccgccgat gtattgaaag
    58741 cttttaatca ttcttataac caagtatcta cctagatata tcttaaatat aatatttaaa
    58801 aagtaggacc atagagaaaa tttatctcat cctacttttt ttgaaaggag aaatatgatg
    58861 aaactaaatg ccattcatca cgttgctatt attgtatctg attatcatct gtcaaaagat
    58921 ttttatgtga ataaactcgg atttgaaatt atcagagaaa attatcgtcc tgacaagcat
    58981 gattacaaat tagatttatc ttgtggtcgt attgaattgg agatttttgg taaggtgaca
    59041 agtgacccca actatcaagc tcctcctaaa cgtgtaagtg aaccagaatt taaaagtgag
    59101 gcctgtggtt tgaggcacct tgcatttcgt gttaccaaca ttgagagtta tgtagatgat
    59161 ctaaagagct taggaattcc agtggagcct atcaggcatg atgattacac aggtgagaaa
    59221 atgactttct tttttgatcc ggatggtttg cctttagaat tacatgaata gacttattag
    59281 gttaacgact ggatcgtgct ataaaactcg ttaaaaatag ttatttgttg tatcttcatt
    59341 tgcttgatta atctcttttg atcgttaaat gatttggtta actttcaaag tgaagagcaa
    59401 tttttggagt aatcttgtcg aagtttttta atagaataac tataggaaaa gcaaaatatt
    59461 tgacgata
//
LOCUS       DAXWIQ010000006        58519 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000006 DAXWIQ010000000
VERSION     DAXWIQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 58519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58519
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.006"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            26..313
                     /locus_tag="ACGEUI_000338"
     CDS             26..313
                     /locus_tag="ACGEUI_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-damage-inducible protein J"
                     /protein_id="HFF8165203.1"
                     /translation="MTTVKKNRAVTFQANKELVSEAMTVLNKKNLTLSSALRLFLQNV
                     VVTNEVDLLTEEELEKEKLFKQFQAEINKNIEDVRQGKFYTSEEVRAELGL"
     gene            303..638
                     /locus_tag="ACGEUI_000339"
     CDS             303..638
                     /locus_tag="ACGEUI_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8165204.1"
                     /translation="MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQRKMEQIISD
                     IEKLEVFPEVGFDADEKYGSKIIHYHSTKGYTLSKDYIVLYHIEGEENRIVIDYLLPT
                     QSDYIKLFK"
     gene            736..1743
                     /locus_tag="ACGEUI_000340"
                     /pseudo
     CDS             736..1743
                     /locus_tag="ACGEUI_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003070648.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1863..2255)
                     /gene="rpsI"
                     /locus_tag="ACGEUI_000341"
     CDS             complement(1863..2255)
                     /gene="rpsI"
                     /locus_tag="ACGEUI_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFF8165205.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLIINQPFAVTSTEGSYDVFVNVVGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2276..2722)
                     /gene="rplM"
                     /locus_tag="ACGEUI_000342"
     CDS             complement(2276..2722)
                     /gene="rplM"
                     /locus_tag="ACGEUI_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFF8165206.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKVYYTHSMYPGGLKSITAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(2940..3146)
                     /locus_tag="ACGEUI_000343"
     CDS             complement(2940..3146)
                     /locus_tag="ACGEUI_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8165207.1"
                     /translation="MKNLKLKAARAGKDLSQQALADLVGVSRQTIAAVEKGDYNPTIN
                     LCIAICRVLDKTLDDLFWEADDSR"
     gene            complement(3143..3649)
                     /locus_tag="ACGEUI_000344"
     CDS             complement(3143..3649)
                     /locus_tag="ACGEUI_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165208.1"
                     /translation="MFDSKQNLAKSQWGFIVITAVMDLVVIVATLLAKTSFQRYLVGG
                     VAFVLTSFLILLVWGLKTAKKLQTRLDSKEVLDSKVRDDQKLPKYDERQKQILLKGYT
                     IGFWFMIVVIWLSLFISRFTEGLVSASFLFTLALWGGLAVQTTYCNLSMGASIFYRHY
                     LDAKEADE"
     gene            complement(3785..4645)
                     /locus_tag="ACGEUI_000345"
     CDS             complement(3785..4645)
                     /locus_tag="ACGEUI_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8165209.1"
                     /translation="MTFTIMTDSTADLNQTWAEDHDIVLIGLTILCDGEVYETVGPNR
                     ISSDYLLKKMKAGSHPQTSQINVGEFEKVFREHARNNKALLYLAFSSVLSGTYQSALM
                     ARDLVREDYPDAVIEIVDTLAAAGGEGYLTILAAEARDSGKNLLETKDIVEAVIPRLR
                     TYFLVDDLFHLMRGGRLSKGSAFLGSLASIKPLLWIDEEGKLVPIAKIRGRQKAIKEM
                     VAQVEKDIADSTVIVSYTSDQGSAEKLREELLAHENISDVLMMPLGPVISAHVGPNTL
                     AVFVIGQNSR"
     gene            complement(4742..5260)
                     /locus_tag="ACGEUI_000346"
     CDS             complement(4742..5260)
                     /locus_tag="ACGEUI_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFF8165210.1"
                     /translation="MKKRILLVDGYNMIAFWQSTRQLFKTNQLDQARNTLLTKLNHYA
                     HFENINIICVFDAQYVPGLRQRYDQYYISVVFTEEDETADSYIERMAAELNTAIHMVE
                     VATSDLNEQWTIFSQGALRVTARELEQRVHTVKADLDKMSRDIDLKTPKLRPFDQGQL
                     IQLKDFMSQLDR"
     gene            complement(5264..6010)
                     /gene="rlmB"
                     /locus_tag="ACGEUI_000347"
     CDS             complement(5264..6010)
                     /gene="rlmB"
                     /locus_tag="ACGEUI_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFF8165211.1"
                     /translation="MEDKDTIETNDIVYGVHAVTESLQANTGNKLYIQEDLRGKKVDN
                     IKSLATQKKVAISWTPKKTLSQMTDGAVHQGFVLRVSAFAYTDVDEILEIAEQEANPL
                     ILILDGLTDPHNLGSILRTADATNVCGVIIPKHRSVGVTPVVSKTSTGAVEHIPIARV
                     TNLSQTLDKLKARGFWIFGTDMNGTPSDCWNTNGKLALVIGNEGKGISTNIKKQVDEM
                     ITIPMNGHVQSLNASVAAAILMYEVFRNRR"
     gene            complement(6057..6854)
                     /locus_tag="ACGEUI_000348"
     CDS             complement(6057..6854)
                     /locus_tag="ACGEUI_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165212.1"
                     /translation="MTTMQKTISLLSLALLIGLLGTSGKAISVYAQDQHTDNVIAEST
                     ISQVSVEASMRGTEPYIDATVTTDQPVRQPTQATITLKDASDNTINSWVYTMAAQQRR
                     FTAWFDLTGQKSGDYHVTVTVHTQEKAVTGQSGTVHFDQNKARKTPTNMQQKDTSKAM
                     TNSVDVDTKAQTNQSANQEIDSTSNPFRSATNHRSTSLKRSTKNEKLTPTASNSQKNG
                     SNKTKMLVDKEEVKPTSKRGFPWVLLGLVVSLAAGLFIAIQKVSRRK"
     gene            complement(7050..7463)
                     /locus_tag="ACGEUI_000349"
     CDS             complement(7050..7463)
                     /locus_tag="ACGEUI_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFF8165213.1"
                     /translation="MTNPVDVNLINGIALAFEGDAVYSYYVRRHLIFQGKTKPSQLHR
                     LATRYVSAKAQANLIQAMLEAQLLTEKEEDIYKRGRNTNSHTKAKNADIITYRMSTGF
                     EAIMGYLDMMGQKERLEELIRWCIEYVEKQQLISS"
     gene            complement(7456..8799)
                     /gene="cysS"
                     /locus_tag="ACGEUI_000350"
     CDS             complement(7456..8799)
                     /gene="cysS"
                     /locus_tag="ACGEUI_000350"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFF8165214.1"
                     /translation="MIKIYDTMTRSLRKFVPLTENTVNIYVCGPTVYNYIHIGNARSA
                     VAFDTIRRYFEYTGYQVNYISNFTDVDDKIIKAATQAGVSPKELSDRFIAAFIEDTKA
                     LGVKPATQNPRVMDYIAEIISFVESLIEKDFAYEADGDVYFRVEKSEHYAKLANKTLS
                     ELEVGASGRTDAETALKENPLDFALWKSAKAGEVSWDSPWGFGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVTVDNEKMSKSLGNF
                     VTVHDMLQTVDGQVLRFFLATQQYRKPINFTEKTIHDAEINLKYLKNTLQQPLTETAD
                     EQELKQFVIAFQDAMDDDFNTANGITVVFDMAKWINSGSYTEPVKSAFEKMLAVFGII
                     FEEEVLEVDIEALIAKRQEARANRDFATADAIRDQLAVQGIKLLDTKDGVRWLRD"
     gene            complement(8827..9057)
                     /locus_tag="ACGEUI_000351"
     CDS             complement(8827..9057)
                     /locus_tag="ACGEUI_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165215.1"
                     /translation="MNKIRVGLYVIMSLSILVSSITLVASLIFLLRQDMVKAIIYFIL
                     TLFWLIGLVINIKLLKRWNSISEKDLGYQDDN"
     gene            complement(9536..10117)
                     /gene="cysE"
                     /locus_tag="ACGEUI_000352"
     CDS             complement(9536..10117)
                     /gene="cysE"
                     /locus_tag="ACGEUI_000352"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFF8165216.1"
                     /translation="MGWWKESIAIVKALDPAARNSLEVILTYPGIKALAAHRLSHFLW
                     RHHFKLLARMHSQFWRFWTQIEIHPGAQIAPGVFIDHGAGLVIGETAIVEKGVMLYHG
                     VTLGGTGKDCGKRHPTVRQGALISAHAQVIGPIDIGANAKVGAAAVVLSDVPEDVTVV
                     GVPAKIVRVHGQKDNRQIQSLQKQREVSYQLSK"
     gene            complement(10126..10878)
                     /locus_tag="ACGEUI_000353"
     CDS             complement(10126..10878)
                     /locus_tag="ACGEUI_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HFF8165217.1"
                     /translation="MTKSNELDIRLRAFINAPDNFLDSLALVNAFHNFPVWAAKEPYV
                     IEVEGVKVTPVFTDKEDMARFKEEQKSAQSQYWLERSALAVLEEVITSGAAGLIFNLK
                     KKGDFGNSTIFKSSDMIQFMNHYTTVLNTLMSDDNVAADTMEKVYLVPAFVYPKDNNH
                     YDRLFPTMSTPEGKSYVPAFSNLQSFAKWYNQDDFGGLFRKAEGVILTWTIDDIYQPR
                     NGENELDETFGVAINPFDDQQILVDWSELDKS"
     gene            complement(10871..13003)
                     /gene="pnp"
                     /locus_tag="ACGEUI_000354"
     CDS             complement(10871..13003)
                     /gene="pnp"
                     /locus_tag="ACGEUI_000354"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFF8165218.1"
                     /translation="MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGDSTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFI
                     INPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQE
                     QIVAVVGKEKAEVELLQVDVDLQADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVK
                     AEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPDGRKIDEIRPLDAVV
                     DFLPKVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLAPEYKKRFLHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDTIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAIDRTKEIIAGLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTTNVSDVLEVGEDVDVKVIKI
                     DEKGRVDASMKALIPRPPKPEKKEEKHD"
     gene            complement(13284..14015)
                     /locus_tag="ACGEUI_000355"
     CDS             complement(13284..14015)
                     /locus_tag="ACGEUI_000355"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFF8165219.1"
                     /translation="MMTKKLNVKVFSDGAVLETMLKDLQTGLVTGFTTNPSLMKKAGI
                     SSYIGFAKEVLAKITDYPVSFEVFADDLASMEKEAEKIASLGDNVYVKIPVTTSTGES
                     TCPLIQKLSAKGIKLNVTAIFTIEQTQAVVDHLTARVPAIVSVFAGRIADTGVDPMPI
                     MEEALRICRQKEGVELLWASPRETYNIYQADQLGVDIITCTTDLIAKLPLQGKDLEDY
                     SLETVQMFLKDSTSLGFKILEDYNH"
     gene            complement(14025..14186)
                     /locus_tag="ACGEUI_000356"
     CDS             complement(14025..14186)
                     /locus_tag="ACGEUI_000356"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165220.1"
                     /translation="MDFGVLGILLGRLGTSIGQIGIYLVVAVLAIVLVLPNFLSKSQE
                     AINNVSVEI"
     gene            complement(14168..15403)
                     /locus_tag="ACGEUI_000357"
     CDS             complement(14168..15403)
                     /locus_tag="ACGEUI_000357"
                     /inference="COORDINATES: protein motif:HMM:NF006921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFF8165221.1"
                     /translation="MEALLSFIRDILKEPAFLMGLIAFAGLVALKTPAHKVLTGTLGP
                     ILGYLMLVAGAGVIVTNLDPLAKLIEHGFSITGVVPNNEAVTSVAQKILGVETMSILV
                     VGLLLNLAFARFTRFKYIFLTGHHSFFMACLLSAVLGAVGFKGSLLIILDGFLLGAWS
                     AISPAIGQQYTLKVTDGDEIAMGHFGSLGYYLSAWVGSKVGKDSKDTEDLQISEKWSF
                     LRNTTISTGLIMVIFYLVATVASVLRNASVAEELAAGQNPFIFAIKSGLTFAVGVAIV
                     YAGVRMILADLIPAFQGIANKLIPNAIPAVDCAVFFPYAPTAVIIGFASSFVGGLLGM
                     LILGVAGGVLIIPGMVPHFFCGATAEIFGNSTGGRRGAMIGASLMAYYSPSCQPCFYL
                     YLVNLVFQTRPLEMWISVF"
     gene            complement(15430..15714)
                     /locus_tag="ACGEUI_000358"
     CDS             complement(15430..15714)
                     /locus_tag="ACGEUI_000358"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8165222.1"
                     /translation="MIKIVTVCGNGIGSSLLLRMKVEAIASSLGIDVDAESCDSNAAV
                     GKGADLFVTVKEFKDIFPEDAKVCIVKSYTNRKKIEEDLVPVLKEMSGKE"
     gene            complement(15707..17767)
                     /locus_tag="ACGEUI_000359"
     CDS             complement(15707..17767)
                     /locus_tag="ACGEUI_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8165223.1"
                     /translation="MLSHELIRNYQLFSKYKGHSLEAFESILKASKRHILADIAKIND
                     TLSLYQLPLIALDRQLVYPPDLTEKDLLNRMLPTLDDYLFQDERLDMIIIYIMMAKEF
                     ISINHLESLLRLSRNSVIADLNLVRDRVQAFQVTLAYNRQDGYFFEGEPLALRRLLES
                     AVSSLLQVTSGPWVFSYLLHELGLPDQKKVMAATLEELSRENHLTFISEKLRDLIYFF
                     CLLAHRPFSRNVRAEAVDTFPLASPAVETMVDQLLVNFPSLTEEKYLVQSRLLGCIQG
                     DLELVFQQPIYDIMEEIINSVAVNTGLSITDTPELRQNLYSHLLPAYYRLYYDINLTN
                     PLKEQIKQDYESLFYLVKRSLSPLEKQLGKSVNEDEVAYFTIHFGRWLQAPKKRPSNQ
                     LVALSVCPNGISSSLMLEATLKELFPQLQFIRIHQLDKIKLLDPASFDLIFSTVAFDC
                     AKPVYVTQALMGPVEKMMLKKMVCDDFHLPLSEQFALDDLLSIIHKHTTITNKEGLVS
                     DLSRYLIGNHLTIEKGGLGLLDLLTADFIRQADAVSDWQEAIRLAAQPLLEHQMIETS
                     YIDGMIDSVNELGAYIVLAPKVAVPHAAPEKGTRQLGMSLLQLKEPVSFDLKQEGDPD
                     KQVQLIFVLSAVDSSSHLKALQELSLILDDDEHIEQLIEAKNTEEIMSLISHMIEKGD
                     ESHD"
     gene            complement(18119..18388)
                     /gene="rpsO"
                     /locus_tag="ACGEUI_000360"
     CDS             complement(18119..18388)
                     /gene="rpsO"
                     /locus_tag="ACGEUI_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFF8165224.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNSHIK
                     QHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(18546..18701)
                     /locus_tag="ACGEUI_000361"
     CDS             complement(18546..18701)
                     /locus_tag="ACGEUI_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165225.1"
                     /translation="MTMLGYRLSAEVRGSAILPLLAGYLIRQAIAQVYSDLKTASWLF
                     GLASSVA"
     gene            18827..19096
                     /locus_tag="ACGEUI_000362"
     CDS             18827..19096
                     /locus_tag="ACGEUI_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8165226.1"
                     /translation="MTQNVCQQNITYTVKERFASYTLEAQANQEVHLNLTLLANRFGT
                     SDRHLKHVLKQPIFQRIIERICFKTYRIADLDALTQLRPLRYAAH"
     gene            19197..19811
                     /gene="def"
                     /locus_tag="ACGEUI_000363"
     CDS             19197..19811
                     /gene="def"
                     /locus_tag="ACGEUI_000363"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFF8165227.1"
                     /translation="MSAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDIL
                     LGEKMMQFLKHSQDPVMAEKLGLRAGVGLAAPQIDVSKRIIAVLVPNLPDKEGNPPKE
                     AYSWQEVLYNPKIVSHSVQDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQH
                     RIKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFETKEELLILD"
     gene            complement(19845..20387)
                     /locus_tag="ACGEUI_000364"
     CDS             complement(19845..20387)
                     /locus_tag="ACGEUI_000364"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFF8165228.1"
                     /translation="MKHILFIVGSLREGSFNHQLAAQAQKALEHQAVVSYLNWKDVPV
                     LNQDIEANAPLPVVDARQAVQSADAIWIFTPVYNFSIPGSVKNLLDWLSRALDLSDPT
                     GPSAIGGKVVTVSSVANGGHDQVFDQFKALLPFIRTSVAGEFTKATVNPDAWGTGRLE
                     ISKETKANLLSQAEALLAAI"
     gene            complement(20518..20946)
                     /locus_tag="ACGEUI_000365"
     CDS             complement(20518..20946)
                     /locus_tag="ACGEUI_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8165229.1"
                     /translation="MSHLDKNTALKAMVVFRKAQRTLDAFGADIFKKADLTATQFSVL
                     EVLYTKGCMRINHLIDSLLATSGNMTVVLNNMERNGWISKCKDKTDKRAYVVTLTDKG
                     TRLIEAVLPKHVARVEEAFAVLTEKEQLCLIELLKKFKQL"
     gene            complement(21056..25453)
                     /locus_tag="ACGEUI_000366"
     CDS             complement(21056..25453)
                     /locus_tag="ACGEUI_000366"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFF8165230.1"
                     /translation="MSDLFAKLMDQIEMPLDMRRSSAFSSADIIEVKVHSVSRLWEFH
                     FAFAAVLPIATYRELHDRLIRTFEAADIKVTFDIQAAQVDYSDDLLQAYYQEAFEHAP
                     CNSASFKSSFSKLKVTYEDDKLIIAAPGFVNNDHFRNNHLPNLVKQLEAFGFGTLTID
                     MVSDQEMTEHLTKNFVSSRQALVKKAVQDNLEAQKSLEAMMPPVEEATPAPKFDYKER
                     AAKRQAGFEKATITPMIEIETEENRIVFEGMVFDVERKTTRTGRHIINFKMTDYTSSF
                     ALQKWAKDDEELRKFDMIAKGAWLRVQGNIETNPFTKSLTMNVQQVKEIVRHERKDLM
                     PEGQKRVELHAHTNMSTMDALPTVESLIDTAAKWGHKAIAITDHANVQSFPHGYHRAR
                     KAGIKAIFGLEANIVEDKVPISYEPVDMDLHEATYVVFDVETTGLSAMNNDLIQIAAS
                     KMFKGNIVEQFDEFIDPGHPLSAFTTELTGITDKHLQGAKPLVTVLKAFQDFCKDSIL
                     VAHNASFDVGFMNANYERHDLPKITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQVSL
                     DHHHMANYDAEATGRLLFIFLKDAREKHGIKNLLQLNTDLVAEDSYKKARIKHATIYV
                     QNQVGLKNMFKLVSLSNIKYFEGVPRIPRTVLDAHREGLLLGTACSDGEVFDAVLTKG
                     IDAAVDLARYYDFIEIMPPAIYQPLVVRELIKDQAGIEQVIRDLIEVGKRAKKPVLAT
                     GNVHYLEPEEEIYREIIVRSLGQGAMINRTIGRGEGAQPAPLPKAHFRTTNEMLDEFA
                     FLGKDLAYQVVVQNTQDFADRIEEVEVVKGDLYTPYIDKAEETVAELTYQKAFEIYGN
                     PLPDIIDLRIEKELTSILGNGFAVIYLASQMLVNRSNERGYLVGSRGSVGSSFVATMI
                     GITEVNPMPPHYVCPSCQHSEFITDGSVGSGYDLPNKPCPKCGTPYQKDGQDIPFETF
                     LGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGDEYAFRAGTVGTVAEKTAYGFVKGYE
                     RDYGKFYRDAEVDRLAAGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAS
                     WQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPITIPADDPGVMALFSGTE
                     VLGVTPEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWLGNA
                     QDLIKEGIATLKTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGY
                     IDAMRENNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHHPIMYYCAYFSIR
                     AKAFELKTMSGGLDAVKARMEDITIKRKNNEATNVENDLFTTLEIVNEMLERGFKFGK
                     LDLYKSDAIEFQIKGDTLIPPFIALEGLGENVAKQIVKARQEGEFLSKMELRKRGGAS
                     STLVEKMDEMGILGNMPEDNQLSLFDDFF"
     gene            complement(25707..27563)
                     /locus_tag="ACGEUI_000367"
     CDS             complement(25707..27563)
                     /locus_tag="ACGEUI_000367"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFF8165231.1"
                     /translation="MKQSKLLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMREEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDNS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHHNYEDLDVTYEDYRQAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAITPART
                     DLDRWVVLDKSIASMDDIPKEVLEDIKAELAAWMISGEDTIAYSTESSYAANLEMATN
                     EYKPSSKVAAEDALAEVETPHCKTIDEVAAFLSVDETQTIKTLLFVADNEPVVALLVG
                     NDHINTVKLKNYLAADFLEPASEEEARAFFGAGFGSLGPVNLAQGSRIVADRKVQNLT
                     NAVAGANKDGFHMTGVNPGRDFQAEYVDIREVKEGEMSPDGHGVLQFARGIEVGHIFK
                     LGTRYSDSMGATILDENGRTVPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGDYRY
                     AWGINFPKELAPFDVHLITVNVKDQVAQDLTAKLEADLMAKGYDVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKAAEGIVEIKIKATGDSIEVNAENLIETLEILTKEH"
     gene            complement(27761..29020)
                     /gene="rseP"
                     /locus_tag="ACGEUI_000368"
     CDS             complement(27761..29020)
                     /gene="rseP"
                     /locus_tag="ACGEUI_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFF8165232.1"
                     /translation="MLGIITFIIIFGILVIVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHVDQGGTLYTLRMLPLGGYVRMAGWGDDKTEIKTGTPTSLTLNEQGFVKRINLSQS
                     KLDPTSLPMHVTGYDLEDQLSITGLVLEETKTYKVAHDATIVEEDGTEIRIAPLDVQY
                     QNASIGGRLITNFAGPMNNFILGIVVFILLVFLQGGMPDFSSNHVRVQENGAAAKAGL
                     RDNDQIVAINGYKVTSWNDLTEAVDLATRDLGPSQTIKVTYKSHQRLKTVAVKPQKHA
                     KTYTIGVKASLKTGFKDKLLGGLELAWSRAFTILNALKGLITGFSLNKLGGPVAMYDM
                     SNQAAQNGLESVLSLMAMLSINLGIFNLIPIPALDGGKILMNIIEAIRRKPIKQETEA
                     YITLAGVAIMVVLMIAVTWNDIMRVFF"
     gene            complement(29093..29887)
                     /locus_tag="ACGEUI_000369"
     CDS             complement(29093..29887)
                     /locus_tag="ACGEUI_000369"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFF8165233.1"
                     /translation="MKERVVWGGVAVAIFLPFLIIGNLPFQLFVGVLAMIGVSELLKM
                     KRLEVFSFEGVFAMLAAFVLAVPMDHYLTFLPIDANVAFYSLMVFFILAGTVLNSRAY
                     SFDDAAFPIATSFYVGIGFQHLINARLSGIDKVFLALFIVWATDIGAYLIGRQFGRRK
                     LLPTVSPNKTIEGSLGGIACAVLVSFIFMVIDRSVYAPHHFLTMLVLVALFSIFAQFG
                     DLVESALKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHLFGLF"
     gene            complement(29900..30649)
                     /locus_tag="ACGEUI_000370"
     CDS             complement(29900..30649)
                     /locus_tag="ACGEUI_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFF8165234.1"
                     /translation="MFGLKAKSTKKVLGSIPKHIGIIMDGNGRWAKKRLKPRVFGHKA
                     GMDALQEVTITASELGVKVLTVYAFSTENWSRPQDEVSFIMNLPVTFFDKYVPVLHEN
                     NVKIQMIGETSRLPEDTLAALNAAIDKTKRNTGLILNFALNYGGRAEITSAVRFIAQD
                     VLDAKLNPGDITEDLIANYLMTDHLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEFY
                     FTPVLWPDFKKAELLKAIADYNRRQRRFGKV"
     gene            complement(30868..31233)
                     /gene="yajC"
                     /locus_tag="ACGEUI_000371"
     CDS             complement(30868..31233)
                     /gene="yajC"
                     /locus_tag="ACGEUI_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFF8165235.1"
                     /translation="MGFPTILMFVVMLGLIWFMQRQQKKQAQERQNQLNAIEKGDEVV
                     TIGGMFAIVDEVDTTAKKIVLDVDGVFLTFELLAIKRIVTKATTETTLVETEETSVVN
                     EPAVETVDSTDTDSAIESH"
     gene            complement(31349..31723)
                     /locus_tag="ACGEUI_000372"
     CDS             complement(31349..31723)
                     /locus_tag="ACGEUI_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HFF8165236.1"
                     /translation="MRPKEKETSMTFEEIVANFIPSSVAEVTSAIASGKDMIVFLGRS
                     SCPYCRRFAPKLAQVATDNQKEVYFVDSENAADAAELAAFRENYQLVTVPALLVSYDQ
                     HQRAVCDSSLTPDDILAFLTRE"
     gene            complement(31838..35335)
                     /locus_tag="ACGEUI_000373"
     CDS             complement(31838..35335)
                     /locus_tag="ACGEUI_000373"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HFF8165237.1"
                     /translation="MKKKVNQGSKRYQYLLKKWGIGFVIAATGTVVLGCTPSILTHQV
                     AAKTIVGLARDEAQQGDGNAKSGDGLQSSSKEAKPVLDSSSANPASIAEHHLRMHFKT
                     LPAGESLGSLGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLAAKHRQQVSY
                     LINNKAGENLSKDQHISLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDN
                     LAVWTFKDVKTPTTDWPNGLDLSHKGHYGAYVDVPLKEGANEIGFLILDKSKTGDAIK
                     VQPKDYLFKELDNHTQVFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTLDG
                     LDEDAVKQNIKITDKAGKTVAIDELTLDRDKSVMTLKGDFKAQGAVYTVTFGEVSQVA
                     RQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADTVKVVVYDKQDQTRVVGQADL
                     TKSDKGVWRAHLTSDSVKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNDATAT
                     DDIKTAKAAFIDPSKLGPTGLDFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHP
                     FGTFSAFVEQLDYLKDLGVTHVQLLPVLSYFYANELDKSRSTAYTSSDNNYNWGYDPQ
                     HYFALSGMYSANPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTARTYLFEDLEPN
                     YYHFMNADGTARESFGGGRLGTTHAMSRRILVDSITYLTREFKVDGFRFDMMGDHDAA
                     AIEQAFKAAKAINPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNT
                     LKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPGNFEADAPGDVVQYIAAHDNLTLHDV
                     IAKSINKDPKVAEEEIHKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYKTKAS
                     DDKVPNKATLIDAVAQYPYFIHDSYDSSDAVNHFDWAKATDSIAHPISNQTKAYTQGL
                     IALRRSTDAFTKATKAEVDRDVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNA
                     DNKTRKVVLPQAYRYLLGAQVLVDAEQAGVTAIAKPKGVQFTKEGLTIEGLTALVLKV
                     SSKTANPSQQKSQTDNHQTKTPDGSKDLDKSLMTRPKRAKTNQKLPKTGEASSKGLLA
                     AGIALLLLAISLLMKRQKD"
     gene            complement(35533..37146)
                     /gene="dexB"
                     /locus_tag="ACGEUI_000374"
     CDS             complement(35533..37146)
                     /gene="dexB"
                     /locus_tag="ACGEUI_000374"
                     /EC_number="3.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043896 - glucan 1,6-alpha-glucosidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan 1,6-alpha-glucosidase DexB"
                     /protein_id="HFF8165238.1"
                     /translation="MKKHWWHKATIYQIYPRSFKDTSGNGIGDLKGITSQLDYLQKLG
                     ITAIWLSPVYQSPMDDNGYDISDYEAIADVFGDMADMDELLAAANERGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDYYIWRDEPNNLMSIFSGSAWELDEASGQYYLHLFSK
                     KQPDLNWENAQLRQKIYDMMNFWIAKGIGGFRMDVIDLIGKVPDLEITGNGPRLHDYL
                     KEMNQATFGNHDVMTVGETWGATPEIARQYSRPENKELSMVFQFEHVGLQHKPDAPKW
                     DYAKELDVPALKAIFSKWQTELKLGEGWNSLFWNNHDLPRVLSIWGNDSTYREKSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFKDLTEVNDIESLNYAKEAMGNGVSAARVMDS
                     IRKVGRDNARTPMQWSKDTHAGFSEAKETWLPVNPNYQDINVADALADPDSIFYTYQK
                     LIALRKEQDWLVEADYHLLPTADKVFAYQRQLGEETYVIVVNVSDEEQVFATDLAGAQ
                     VIIANTDVDTVLETKHLQPWDAFCLKLKA"
     gene            complement(37275..38408)
                     /locus_tag="ACGEUI_000375"
     CDS             complement(37275..38408)
                     /locus_tag="ACGEUI_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8165239.1"
                     /translation="MVELNLNHIYKKYPNTTHYAVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIGGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYKKDDIDRRVKEAAQILGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPQGN
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPAMNFFEVEVKDGRIVSEDGLDIAIPEG
                     QAKMLEAAGYKGEKVTFGIRPEDISSRQIVHDTYPSATVTAEVLVSELLGSETMLYVK
                     LGQTEFASRVDARDFHSPGEQVSLTFNVAKGHFFDRDTEQAIR"
     gene            complement(38706..39554)
                     /locus_tag="ACGEUI_000376"
     CDS             complement(38706..39554)
                     /locus_tag="ACGEUI_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8165240.1"
                     /translation="MELKDYFPEMQVGPHPLGDKDWMSIKEGDQYVHFPKSCLSEKER
                     LLLEVGLGQCEVLQPLGSPWQRYLLDHQGNPPQLYETSQFIYLNHQQALPDDLVELLQ
                     QMIAGLEVILPISATQTAFLCRQAISIKVLRWLEDLLPTLESDFGLALTMFVGNAWYQ
                     VAAGTLRECFEEECQLLTAYLRQQSGRKLLTFSGLMLWSLLSHHTFLALTRQFHQFLS
                     PQSDMADVVHALWSEHGNLVQTAQRLYIHRNSLQYKLDKFAQQSGLHLKQLDDLAFAH
                     LFLLKY"
     gene            39914..41236
                     /gene="ska"
                     /locus_tag="ACGEUI_000377"
     CDS             39914..41236
                     /gene="ska"
                     /locus_tag="ACGEUI_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptokinase"
                     /protein_id="HFF8165241.1"
                     /translation="MKNYLSIGVIALLFALTFGTVKSVQAIAGYGWLPDRPPINNSQL
                     VVSMAGIVEGTDKKVFINFFEIDLTSQPAHGGKTEQGLSPKSKPFATDNGAMPHKLEK
                     ADLLKAIQKQLIANVHSNDGYFEVIDFASDATITDRNGKVYFADKDGSVTLPTQPVQE
                     FLLKGHVRVRPYKEKPVQNQAKSVDVEYTVQFTPLNPDDDFRPGLKDTKLLKTLAIGD
                     TITSQELLAQAQSILNKTHPGYTIYERDSSIVTHDNDIFRTILPMDQEFTYHVKNREQ
                     AYEINPKTGIKEKTNNTDLVSEKYYVLKQGEKPYDPFDRSHLKLFTIKYVDVNTNELL
                     KSEQLLTASERNLDFRDLYDPRDKAKLLYNNLDAFDIMDYTLTGKVEDNHDKNNRVVT
                     VYMGKRPKGAKGSYHLAYDKDLYTEEERKAYSYLRDTGTPIPDNPKDK"
     gene            complement(41334..41777)
                     /gene="dtd"
                     /locus_tag="ACGEUI_000378"
     CDS             complement(41334..41777)
                     /gene="dtd"
                     /locus_tag="ACGEUI_000378"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFF8165242.1"
                     /translation="MKLVLQRVKEASVSIDGKIAGAINQGLLLLVGVGPDDNAEDLAY
                     AVRKIVNMRIFSDADGKMNQSIQDIKGSILSVSQFTLYADTKKGNRPAFTGAAKPDLA
                     SQLYDSFNEQLAEFVPVERGVFGADMQVSLINDGPVTIILDTKCH"
     gene            complement(41792..44011)
                     /locus_tag="ACGEUI_000379"
     CDS             complement(41792..44011)
                     /locus_tag="ACGEUI_000379"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFF8165243.1"
                     /translation="MAKIMNVTGEEVIALAATYMTKADVAFVAKALAYATAAHFYQVR
                     KSGEPYIVHPIQVAGILADLHLDAVTVACGFLHDVVEDTDITLDEIEADFGHDARDIV
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLAFRYLNETEFYKISHMMKEKRREREALV
                     EAIVSKVKTYTTQQGLFGDVYGRPKHIYSIYRKMRDKKKRFDQIFDLIAIRCVMETQS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKDMHQV
                     AEYGVAAHWAYKKGVRGKVNQAEQAVGMNWIKELVELQDASNGDAVDFVDSVKEDIFS
                     ERIYVFTPTGAVQELPKESGPIDFAYAIHTQIGEKATGAKVNGRMVPLTAKLKTGDVV
                     EIITNANSFGPSRDWVKLVKTNKARNKIRQFFKNQDKELSVNKGRDLLVSYFQEQGYV
                     ANKYLDKKRIEAILPKVSVKSEESLYAAVGFGDISPISVFNKLTEKERREEERAKAKA
                     EAEELVKGGEVKHENKDVLKVRSENGVIIQGASGLLMRIAKCCNPVPGDPIDGYITKG
                     RGIAIHRSDCHNIKSQDGYQERLIEVEWDLDNSSKDYQAEIDIYGLNRSGLLNDVLQI
                     LSNSTKSISTVNAQPTKDMKFANIHVSFGIPNLTHLTTVVEKIKAVPDVYSVKRTNG"
     gene            complement(44263..45309)
                     /gene="slc1"
                     /locus_tag="ACGEUI_000380"
                     /gene_synonym="slcA"
     CDS             complement(44263..45309)
                     /gene="slc1"
                     /locus_tag="ACGEUI_000380"
                     /gene_synonym="slcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin Scl1"
                     /protein_id="HFF8165244.1"
                     /translation="MLTSKHHNLNKLVWRYGLTSAAAVLLAFGGGASSVKAEVSSTTM
                     TSSQRESKIKEIEESLKKYPEVSNEKFWERKWYGTYFKEEDFQKELKDFTEKRLKEIL
                     DLIGKSGIKGDRGETGPAGPAGPQGKTGERGAQGPKGDRGEQGIQGKAGEKGERGEKG
                     DKGETGERGEKGEAGIQGPQGEAGKDGAPGKDGAPGEKGEKGDRGETGAQGPVGPQGE
                     KGETGAQGPAGPQGEAGKPGEQGPAGPQGEAGQPGEKAPEKSPEGEAGQPGEKAPEKS
                     KEVTPAAEKPADKEANQTPERRNGNMAKTPVANNHRRLPATGEQANPFFTAAAVAVMT
                     TAGVLAVTKRKENN"
     gene            45691..46173
                     /gene="nrdI"
                     /locus_tag="ACGEUI_000381"
     CDS             45691..46173
                     /gene="nrdI"
                     /locus_tag="ACGEUI_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFF8165245.1"
                     /translation="MPQITLVFISLSGNTLSFVKRLSLYLADNYDYHVKQINIKDLKH
                     ETFPVKEEFVAILPTYLEGGNGVDSGDVEILTTPLGEFIAAHGNAQRCLGIIGSGNKN
                     FNHQYCLTAKQYAKRFGFPLLGDFELRGTPDDISRLAQVIMEASSRHSSNDTQTLPNS
                     "
     gene            complement(46567..47385)
                     /locus_tag="ACGEUI_000382"
     CDS             complement(46567..47385)
                     /locus_tag="ACGEUI_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFF8165246.1"
                     /translation="MTKVLTLNTHSWMQANTLKKLVALAEHILAEKYDIICLQEINQL
                     IESELATDLPRYQALSGTPSIHKDHFALLLIHYLQKRGQHYYWSWAYNHIGYDIYQEG
                     VAILSKQPIHVSDILVSAMDDETDYHTRRSLIAKTTLDGKEVAVVNVHLSWFDKGFLG
                     EWEKLEKELLTLNCPLLLMGDFNNPTDQDGYQVMMGSPLDLQDSHKGADHVFGDHSIV
                     ADIDGWQGNKEALKVDHVFTSKDFIIRSSKITFEGGDAPVVSDHYGLEVTLGWR"
     gene            complement(47468..49654)
                     /locus_tag="ACGEUI_000383"
     CDS             complement(47468..49654)
                     /locus_tag="ACGEUI_000383"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFF8165247.1"
                     /translation="MKTSFKQLFRFEFWQKFGKCLMVVIAVMPAAGLMISIGNSIPMI
                     NHDSAFLASLGNIIAQIGWAVIVNLHLLFALAIGGSWAKERAGGAFASGLAFVLINRI
                     TGAFYGVSSTMLADPEAKITSLLGTQMIVKDYFTSVLESPALNTGVFVGIIAGFVGAT
                     AYNKYYNYRKLPEVLTFFNGKRFVPFVVILRSIFVALILVVVWPVIQSGINSFGMWIA
                     SSQDSAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYEVMTGAAAGTKVFGQ
                     DPLWLAWVTDLVHLKGSDASAYSHLMDSVTPARFKVGQMIGATGTLMGVALAMYRNVD
                     ADKKHTYKMMFISAAAAVFLTGVTEPLEYLFMFAAMPLYIVYALVQGASFAMADLVNL
                     RVHSFGNIELLTRTPMALKAGLGMDVINFVWVSVLFAVIMYFIADMMIKKMHLATAGR
                     LGNYDADILGDRNTQTRPTQVADSNSQVVQIVNLLGGAGNIDDVDACMTRLRVTVKDP
                     AKVGAEDDWKKAGAIGLIQKGNGVQAVYGPKADILKSDIQDLLDSGALIPEVNMSQLT
                     SKPTPAKDFKHVTEDVLSVADGMVLPITGVKDQVFAAKMMGDGFAVEPTHGNIYAPVA
                     GLVTSVFPTKHAFGLLTDNGLEVLVHVGLDTVALNGVPFSVKVSEGQRVHAGDLLVVA
                     DLAAIKSAERETIIVVAFTNTTEIQDVTLTSLGAQPAKTKVATVEL"
     gene            complement(50012..50761)
                     /locus_tag="ACGEUI_000384"
     CDS             complement(50012..50761)
                     /locus_tag="ACGEUI_000384"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFF8165248.1"
                     /translation="MQQYFIKGKAEKKVTITDKDTIKHMFQVMRLADEAEVVLVFDDG
                     VKYLAKVTNSMAHELEIIEALPDQVELPVKVTIASGFPKGDKLDTIAQKVTELGASAL
                     WGYPADWSVVKWDGKKLAKKEDKLAKIVLGAAEQSKRNRVPEVHLFEHKAEFLKSLSS
                     FDHIFIAYEETAKAGQLATLAREVKEVKPGAKILFIFGPEGGISPTEITQFEAASAIK
                     VGLGPRIMRAETAPLYALSALSYALELQASK"
     gene            complement(50761..51714)
                     /gene="prmA"
                     /locus_tag="ACGEUI_000385"
     CDS             complement(50761..51714)
                     /gene="prmA"
                     /locus_tag="ACGEUI_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFF8165249.1"
                     /translation="METWQEVTVHVHRDAQEAVSHVLIETGSQGVAIADSADYIGQKD
                     RFGELYPDVEQSDMIAITAYYPSSTNLADIIATINEQLAELASFGLQVGQVTVDSQEL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYDASAGEKVIKLDPGMAFGTGTHPTTKM
                     SLFALEQILRGGETVIDVGTGSGVLSIASSLLGAKTIYAYDLDDVAVRVAQDNIDLNQ
                     GTDNIHVAAGDLLKGVSQEADVIVANILADILVLLTDDAYRLVKKEGYLILSGIISEK
                     LDMVLEAAFSAGFFLETHMVQGEWNALVFKKTDDISGVIGG"
     gene            complement(51785..52255)
                     /locus_tag="ACGEUI_000386"
     CDS             complement(51785..52255)
                     /locus_tag="ACGEUI_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFF8165250.1"
                     /translation="MATYGFLSVLEEEMDKHFQYDYAMDWDKKNHAVEVTFVLEAQNK
                     EAIKTIDDSGEVTQDDIVFEDYVLFYNPAKSQFDAADYLVTIPFDAKKGFSREFLAYF
                     AQFLNDVAIEGHSDLMDFLADDSKADFFLEWNAQAFEEGQQGLEEAASYPYPRY"
     gene            52455..54212
                     /gene="pabB"
                     /locus_tag="ACGEUI_000387"
     CDS             52455..54212
                     /gene="pabB"
                     /locus_tag="ACGEUI_000387"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HFF8165251.1"
                     /translation="MHRKTIIDFKELGQRYLFDEPLVELVAKSLDQVGPVIEKVQHYQ
                     QLGYYVVGYLSYEAAAFFDNALQTHNDRLGNEYLAYFTVHKTCQKKDLPLDYDSITIP
                     NQWVSATQKEAYQKAIETIHREMQQGNTYQVNYTLQLTQELNAADSLAIYNKLVVEQA
                     AGYNAYIAHDEFAVISASPELFFKQEGNRLTTRPMKGTTKRGVNSWLDQQEHDWLQAD
                     GKNRSENMMIVDLLRNDMGKICQTGSVRVDRLCEVERYSTVWQMTSTIVGDLKADCDL
                     IDILKALFPCGSITGAPKVSTMAIITSLEPKPRGIYCGSIGICLPDGRRFFNVPIRTI
                     QLSHNQATYGVGGGITWQSKWEDEYEEVHQKTAFLYRHKQIFDLKTTAKVEHKKIAFL
                     EQHLNRLKEAATYFAYPYNEKALQKQLSTYLENKNNAAYRLMIRLSKDGKISLSDQPL
                     EPLSADFLTAQLSLQKKDVTASPFTYFKTSYRPHIEQKSYEQLFYNQAGQLLETSIGN
                     LFVQLGQTLYTPPVAVGILPGLFRQELLATGQAQEKEVTLADLKEASAIFGGNAVRGL
                     YPLNLELTHLDALLAKSQA"
     gene            54245..54811
                     /locus_tag="ACGEUI_000388"
     CDS             54245..54811
                     /locus_tag="ACGEUI_000388"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFF8165252.1"
                     /translation="MILLIDNYDSFTYNLAQYLSEFDETIVLYNQDPNLYDMAKKANA
                     LVLSPGPGWPKEANQMPKLIQDFYQTKPILGVCLGHQAIAETLGGTLRLAKRVMHGRQ
                     STIETQGPASLFRSLPQEITVMRYHSIVVDQLPKGFSVTARDCDDQEIMAFEHHTLPL
                     FGLQFHPESIGTPDGMTMIANFIAAIPR"
     gene            54844..56112
                     /locus_tag="ACGEUI_000389"
     CDS             54844..56112
                     /locus_tag="ACGEUI_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFF8165253.1"
                     /translation="MPDHLALRMRPKTISEVIGQKHLVGEGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTRYAFRTFNATIDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGTIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKTAIQ
                     LAISDKERGFPFLVTIDDEALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDGSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPDAQVHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYL
                     AMDAALADLRTSGNLPIPRHLRDGHYAGSKDLGNAKDYLYPHAYPEKWVKQQYLPDKL
                     VGHHYFEANETGKYERALGSNKERIDKLSD"
     gene            56201..56397
                     /gene="ssrS"
                     /locus_tag="ACGEUI_000390"
     ncRNA           56201..56397
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACGEUI_000390"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            56398..56470
                     /locus_tag="ACGEUI_000391"
     tRNA            56398..56470
                     /locus_tag="ACGEUI_000391"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56431..56433,aa:Lys,seq:ctt)
     gene            56609..57049
                     /locus_tag="ACGEUI_000392"
     CDS             56609..57049
                     /locus_tag="ACGEUI_000392"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8165254.1"
                     /translation="MKIIEYKGNYRDDLIFMVLEAKNALRRIPSINPDLLTIETSYLE
                     KEDCFWLAVNEESRVIGSIAYSSIPKTNEVFLHRLFIKPSLKHQGIGSQLLAFAEDYL
                     RKIGKSHVRIHLGQPKEQWFESYRFYPKHGYQFYDDTHLIKKLI"
     gene            57104..57610
                     /locus_tag="ACGEUI_000393"
     CDS             57104..57610
                     /locus_tag="ACGEUI_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HFF8165255.1"
                     /translation="MKIAIIGHSGSGKSTLARFLGQHYHCEVFHLDQLHFSSNWQERS
                     DHDMIADLSTCLLKQDLIIEGNYANCLYEERMSEADYIIYVNFSRFHCVYRAFKRYLN
                     YRGKTRPDMADNCQEKFDVAFVKWILLDGRSRNQLKKYQSVVQKYSHKTIVLTNQKQL
                     SHYMNTIN"
     gene            57859..>58519
                     /gene="smeZ"
                     /locus_tag="ACGEUI_000394"
     CDS             57859..>58519
                     /gene="smeZ"
                     /locus_tag="ACGEUI_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal mitogenic exotoxin SmeZ"
                     /protein_id="HFF8165256.1"
                     /translation="MKKTKLIFSFTSIFIAIISRPVFGLEVDNNSLLRNIYSTIVYEY
                     SDTVIDFKTSHNLVTKKLDVRDARDFFINSEMDEYAANDFKDGDKIAMFSVPFDWNYL
                     SEGKVIAYTYGGMTPYQEEPMSKNIPVNLWINRKQIPVPYNQISTNKTTVTAQEIDLK
                     VRKFLISQHQLYSSGSSYKSGKLVFHTNDNSDKYSLDLFYVGYRDKESIFKVYKDNKS
                     FN"
BASE COUNT        17082 a        13113 c        11092 g        17232 t
ORIGIN      
        1 aagttacata aaggggttac ataaaatgac tacagtaaaa aaaaacagag cggttacctt
       61 tcaagctaat aaagaattgg taagcgaagc aatgacagta ttaaacaaaa aaaatttaac
      121 cttatcatct gctttaagat tattcctaca aaatgtcgtt gtcacaaacg aggttgactt
      181 attgacggaa gaggagttag agaaagaaaa acttttcaag caatttcaag ctgaaatcaa
      241 caaaaatatt gaggatgttc gtcaagggaa gttttatacc tctgaggaag tgagggctga
      301 acttggacta taagaaatat cagattatct atgctcctga tgttttagag aagctaaaag
      361 aaattcgtga ttatatttct caaaactatt cctcaacatc aggtcagcgt aaaatggagc
      421 aaatcattag cgacatagaa aaactcgagg ttttcccaga agttggtttt gatgccgatg
      481 aaaaatatgg ttcaaaaatc atccattatc acagcactaa aggttatacc ttaagtaaag
      541 attatattgt cctataccat atcgaggggg aagaaaatag gatcgttatt gattacttgc
      601 ttcctactca aagcgactat ataaagttat tcaaataaca cgttaaaagc cttagcagtc
      661 ttgctagggc ttttttggta gattaccaat taactaggag caaaatgagt tcgggctgtt
      721 tagtagatat tccacaaaga agccaaagac tacgaagcta ggctaatcta ccttaaggct
      781 tcaggaaact tggaggagtt caccaagcca actcataaaa cttatagcga gatattcgag
      841 aaatggtatc aagcctatca agatacggta gagccaacca cggcttctcg tactcttgat
      901 ctgtttcgac ttcacattct gccagtcatg ggagaattgc ccattaacaa aacctcccct
      961 ttagactgct agaacttcat caccgataaa gcaaagacct ttaagaatat caagcaaatc
     1021 aagtcctaca ctgaaaaggt ctttgatttt gccatcaaga tgaaactgct caagcataac
     1081 cctatggctg aaatcattat gccaaagcgg aaaaagacac gaatagaaaa ctattggacg
     1141 gttcaagaac ttcaggagtt tttagccata gtacttcaga aagagcctta caagcactac
     1201 gcactatttc ggctactagc ttatagcggc agctatgcgc tcttaaatgg gctaatatag
     1261 acactaagcg tcaataagag cctaggacga ttagacggac aggcaatcga aaaaagcact
     1321 aaaaatgatt tctcagtaag aaagattaag ctagacagcg aaactatttc tattctgcaa
     1381 gaatggaaag ccatcagcca aaaagaaaag acacaactag cagttgcacc tctttcaatt
     1441 aagtctgact ttatatttac atactgtact agagaaggga gtattgaacc acttcatgca
     1501 gactatatta acaatatctt atcaaaggtc attaagcaac atagtcttaa aaaaataagt
     1561 cctcacagct tcaggcatac ccatgcaacc ctgatgattg agattggagt tgacccagta
     1621 aatacagcca aaaggttagg acatgctagt agtcaaatga ctcttgatac atacagtcat
     1681 gctactgcag tcggagaaga caaatcaatc aaacaatttg ctaactgtct caaaaataac
     1741 taaataaacg tattaaacca agtcaaaagt tttccgaaac ggtatcaaaa gcaaaaaacg
     1801 acaaaaaaag caccttgcaa gagacttgca aaatgcttgt aatcgttgat gtaaagctgt
     1861 tcttaacgtt ttgagaattg tgaagcttta cgagctttct taagacctgg ttttttacgt
     1921 tcaaccatac gtgcgtcacg tgtaagaagg ccagcgcgtt tcaatgaatc gcggaagtct
     1981 gggtctactt gaagaagcgc acgagcgata ccatgacgga tcgcacctga ttgtccaccg
     2041 taaccaccac caacaacgtt aacgaaaacg tcgtatgaac cttcagttga tgtaactgca
     2101 aaaggttggt tgatgatcaa acgtaagtca gcatgtggga tgtattcttc tacatctttt
     2161 ttgttaacag tgattttacc agtacctgga accaaacgaa cgcgtgcaac agcgttttta
     2221 cgacggccag tacctgcata ttgtgcttgt gccattattt aatgctcctt tcctcttaga
     2281 taagtcctga gatgtcaagt acttctggtt gttgtgctgc gtgagtatgc tcaccgccaa
     2341 cgaagacttt caatttcata ccttgtgcac gtccaagagt gttatgtgga agcatgcctt
     2401 taactgattt ttcaatcaag cgaactgcgt ttttagagcg aagttcacca gcagtgattg
     2461 atttcaaacc acctgggtac attgagtgag tgtagtaaac tttatcagtt gcttttttac
     2521 cagttaattt aactttttca gcgttgataa cgataacaaa atcacctgta tcagtatgtg
     2581 gtgtgaaagt tggtttgttt tttccgcgaa gtacgctagc aactactgca gaaagacgtc
     2641 caagtggcac atcagttgcg tcaacaacgt accatttgcg ttcaacttgg cctggtttag
     2701 ccatgaaagt tgttttgttc atgatttctc ctatacgaat tcgtaatatt tgtttacaag
     2761 gtgatgggtg ttccgtccca tcgaaggtat ttggaaggtt ccggggcctt tcaaatgggg
     2821 taaacaatac cgcctactat gatatcaaac ttaagaggct ccagtcaagt gctttagctg
     2881 attttattgg attatttttt ctttcgattt aggagaatgg cagagctgct ctgctggtgt
     2941 taacgagaat catcagcttc ccaaaaaagg tcatctaaag tcttatcaag cactcgacaa
     3001 atggcaatgc aaagattaat cgttgggttg taatcacctt tttcaacagc cgcaatggtt
     3061 tgtctggaga cgcctaccaa atctgctaga gcttgttgag acaaatcttt gcctgcacgc
     3121 gctgctttta atttgaggtt cttcattcat ctgcctcttt agcatccaga taatgacgat
     3181 agaaaatact agctcccata ctaaggttac aatatgtcgt ttgaactgcc aaccctcccc
     3241 aaagagccaa tgtaaagagg aagcttgcag ataccagtcc ctctgtgaat cggctaataa
     3301 agagtgacag ccagataacc acaatcataa accaaaaccc aatagtgtat cctttgagaa
     3361 gaatctgttt ttggcgttca tcatatttgg gcaacttctg atcgtccctt accttagaat
     3421 ctagtacttc cttgctgtct agtctcgttt ggagcttttt cgctgtcttt agcccccata
     3481 ccaacaatat gagaaagcta gtcaatacaa aagctacgcc tccaactaag taacgttgaa
     3541 aactggtctt agccagtaat gttgctacta tcacaactag atccatcact gctgttatga
     3601 caatgaaacc ccattgtgac ttagcgagat tttgtttgct atcaaacatg attcgtttct
     3661 cctttaaatc tttatatgac ataatataaa ttattttgga tattttgtca aatatatttt
     3721 acataattga ttgacttaag cagcaaaaaa ggattgagga tttctcttca atccttataa
     3781 gggattaacg ggaattctgc ccaatcacaa aaactgccag ggtgttagga ccaacgtgag
     3841 ctgagataac tggtcctagt ggcatcataa gaacatcgct aatattctca tgcgccagca
     3901 gctcttctcg taacttttcg gcactaccct gatcgcttgt ataagagaca ataaccgtcg
     3961 aatccgcaat atctttttcc acttgagcca ccatttcttt gatggctttt tggcgtcccc
     4021 gaatttttgc aatgggcacc aatttccctt cctcatcaat ccacaaaaga ggtttgatac
     4081 tggctaaact gcctagaaaa gctgaacctt ttgacagacg cccaccacgc atcaaatgaa
     4141 aaagatcatc tactaagaaa taggtgcgca gtcgagggat cactgcttca acaatatctt
     4201 tagtctccag taaatttttg ccactatctc tggcttctgc tgctaaaatg gttagatagc
     4261 cttctcctcc tgcagcagcc aaggtatcaa caatttcaat caccgcatcg ggataatctt
     4321 ctcgaacaag atcgcgtgcc attagagcgc tttgataggt acccgataaa accgaagaaa
     4381 aagcaagata aagcagtgct ttgttgttcc tagcatgttc acgaaaaacc ttctcaaact
     4441 ctcccacatt aatctgactg gtttgaggat gactgccagc tttcatcttt ttcaaaaggt
     4501 aatcactact aatacggtta ggaccgaccg tttcataaac ctctccatcg cacaaaatcg
     4561 ttaatcctat aaggacaata tcatgatctt ctgcccaggt ttgattcaaa tcagcggttg
     4621 aatctgtcat tattgtaaag gtcattctta cttctcctca tccagttttt tagtttgata
     4681 atcaaataat tattgtttta ttttatcaaa aaaacccgca cttggcgagt cgtaattctc
     4741 attatcgatc taactgagac ataaaatcct tcaattgaat aagctgtcct tggtcaaaag
     4801 gccgcagttt aggcgttttg agatcaatat ctctagacat tttatctaaa tcagctttga
     4861 cagtatgaac tctttgctct aattctcttg cagtgacacg aagagctcct tgagaaaaaa
     4921 tcgtccattg ttcatttaaa tcactcgtag ctacttctac catatgtata gccgtattta
     4981 actctgctgc catgcgctca atgtagctat ctgctgtttc gtcttcctct gtaaatacca
     5041 ctgagatata atactggtca tatcgttgcc ttaaccccgg tacatattgg gcatcaaaaa
     5101 cacaaataat attaatattc tcaaaatggg cataatgatt aagttttgtt aaaagtgtgt
     5161 tacgtgcctg atcaagctga tttgtcttaa acaactgacg ggttgattgc caaaaggcaa
     5221 tcatattata cccatctact aataatatcc gttttttcat aatttagcgc ctatttcgga
     5281 aaacttcata catgagaata gctgccgcta cgctagcatt taagctctgt acgtgaccat
     5341 tcataggaat cgtaatcatt tcatcaactt gctttttgat attggtagag atgcctttac
     5401 cttcgttacc aatgactaaa gcaagtttac cattagtgtt ccagcaatca gacggtgttc
     5461 cattcatgtc tgtgccaaaa atccagaatc ctcttgcttt caatttatct agagtttgac
     5521 taaggttagt tactctcgct atcggaatat gttcaacagc acctgtagac gtctttgaaa
     5581 ccactggagt cacaccaaca gaacgatgtt taggaataat cactccacat acatttgtag
     5641 catcagctgt ccgtaaaatc gatcctaaat tatgaggatc tgtcagacca tctaaaataa
     5701 gaatcaaagg atttgcttct tgctctgcta tttcgaggat ctcatcaaca tcagtatagg
     5761 caaaagctga tactctcaga acaaaacctt gatgcacagc tccatcagtc atttgtgaca
     5821 aggttttttt aggcgtccat gaaatagcga cctttttttg tgtcgctaag cttttgatgt
     5881 tatccacttt ctttcctctt aaatcctctt ggatataaag cttatttcct gtatttgctt
     5941 gaaggctttc tgtaacggca tgaacaccat agacgatatc gtttgtttca atagtatctt
     6001 tatcttccat gagtttatta taccatgaat gcttcaaaat aaatgcatag actcttttat
     6061 tttcgtctag atactttttg aatagctata aataaacctg cagctaaact gactactaga
     6121 cctaataaga cccaagggaa tcctcttttt gaagtaggtt ttacttcctc tttgtccact
     6181 agcatttttg tcttgttgct accgtttttt tggctattac tagcagttgg tgtaagtttc
     6241 tcatttttag tagatcgctt taaggaagtt gatcgatgat tagtagctga tctgaaagga
     6301 tttgaagtag aatctatttc ttggttagct gattgatttg tttgagcttt tgtgtctaca
     6361 tcgactgaat tcgtcattgc tttagaagta tccttttgtt gcatattagt tggtgttttt
     6421 ctagctttgt tttgatcaaa atgaacagtt cctgattgac cagttactgc cttttcttga
     6481 gtatgaacgg tgacagttac atgatagtca ccactctttt gtccagttaa atcaaaccaa
     6541 gctgtaaaac gacgctgttg cgctgccata gtatataccc aactattaat agtattatca
     6601 ctagcgtctt taagtgttat cgttgcctga gttggttgtc tgacaggttg atctgtggtg
     6661 actgtagcat caatataagg ttctgttcca cgcatactgg cttcaacact gacctgacta
     6721 atagttgatt cagctataac attatcagtg tgctgatctt gtgcatacac agatatggct
     6781 ttgccagaag tccccagcaa accaataagt aaagctagtg ataataagct aattgttttt
     6841 tgcatagttg tcataataaa tatctcctct ttaaattaat cttaccctct cattataaac
     6901 tattcgaaaa tgatttcatt atttttatgt cttttttgtt acaaatgtat cttttttata
     6961 tcattatgtc tttttgatgt cataaaaaga cttaatggct ctctaccttt acttcagacc
     7021 tatgaaaata agagtctcct aggggactct tatgaggata tcaattgttg cttttctaca
     7081 tactcaatac accatctgat caattcttct aaccgctctt tttggcccat catatcaaga
     7141 taacccataa tggcttcaaa acctgtcgac atacgatagg taataatatc ggcattctta
     7201 gctttagtat ggctattggt attgcgacca cgcttataga tgtcttcttc tttttcggtc
     7261 aatagctgcg cttctaacat agcctgaatc aagttggctt gtgccttagc agaaacatat
     7321 ctcgttgcta aacggtgtag ctggctaggt ttcgttttac cttgaaaaat gagatgacga
     7381 cgaacatagt aggaataaac cgcatcccct tcaaaggcta gggcaatgcc attaatcaaa
     7441 ttcacatcaa ctggattagt cacgcagcca cctcacacca tctttggtat ctagcagctt
     7501 gatgccttga actgctaact gatctcgaat ggcatcagct gtggcaaaat cacgattcgc
     7561 gcgtgcttct tgtcgcttgg caatcaaggc ttcaatgtca acctcaagca cctcttcttc
     7621 aaagataatg ccaaaaacag ctaacatttt ctcaaaagcg cttttaacag gttctgtata
     7681 ggagccagaa ttaatccact tggccatgtc aaatacgacg gtaatcccat ttgctgtatt
     7741 gaaatcatcg tccatagcgt cttggaaagc tattacaaac tgttttagct cttgctcatc
     7801 tgcagtttct gtcagcggtt gttgcaaggt gtttttaaga tacttcagat tgatttccgc
     7861 atcatgaata gtcttttcag tgaagttaat tggttttcgg tactgttgtg tcgcaagaaa
     7921 gaatcgcaaa acctgaccat caacggtttg taacatgtca tgaacggtca caaaattacc
     7981 tagtgacttg gacatttttt cgttatcaac agtgacaaac ccattgtgca tccaatagtt
     8041 agcaaaagtc ttgcctgttt tggcctcaga ttgggcaatt tcattggtat gatgaggaaa
     8101 ttccaaatca gctccgccac catgaatatc aatggtatca ccaagaattt cagtagccat
     8161 cacagaacat tcaatgtgcc agcctggacg accaaacccc caggggctat cccaagaaac
     8221 ctcacctgcc ttagctgatt tccaaagagc aaagtccagt ggattttctt ttaaagctgt
     8281 ctcagcatct gttcgaccac tggctccaac ttcaagttct gacagggttt tattagctag
     8341 cttggcataa tgctctgact tttccacgcg gaaatacaca tctccatctg cttcgtaagc
     8401 aaaatctttt tcaatgagac tttcaacaaa tgaaatgatt tctgctatat aatccataac
     8461 acgaggattt tgtgtggctg gcttaacacc aagtgccttg gtatcttcta taaaagctgc
     8521 aataaagcga tctgacaatt ctttaggaga aacacctgct tgagtagcag ccttgataat
     8581 tttatcatcg acatcggtaa aattggaaat gtaattgacc tgatagccag tatactcaaa
     8641 ataacgtcga atggtgtcaa aagcgaccgc tgatctagca tttccaatat gaatataatt
     8701 atagaccgtc ggtccacaaa cgtatatatt gactgtattt tcagtcaaag gtacaaactt
     8761 gcggagcgaa cgggtcatgg tatcataaat tttaatcatg taaactcctt attcaatagt
     8821 ctggcattag ttgtcatctt gatatcctaa gtctttttct gaaatgctgt tccacctttt
     8881 caacaattta atattaataa ccaaacctat taaccaaaat agggtcaaaa tgaagtatat
     8941 aatcgcttta accatgtctt gtctcagtaa aaagattaga ctagccacca gtgtaatact
     9001 agaaacaaga atagacaaac tcataatgac atacagccca actcttattt tattcatgag
     9061 gctcctttat taacgaaaaa ccttcacaac ctgtgaaggc ttcagaaagt agacaaaccc
     9121 acttttatgc tcaaaaagca tgaatagatc tgctgatttg acatcaggtt tcctatttct
     9181 gcatgtgggg tgagccaaaa cagctgcttt ttgttgtggt aaaagtggta tcaatactga
     9241 cagtgatgcc catacagatt tttgagacct taggctcaaa actaaggcat ggaattccaa
     9301 aggaagtcgc tgccgtaagc accacctgag aaaaagtatc acaatagact gtttcttcag
     9361 tctaagactt gcttagttga tgctctttta cgtgtttttt gtattttgta acattcccat
     9421 atcactacac tcagcaacca aactgatagg gctagctaag ttcaacatta ctctactaga
     9481 aagtcccaaa atattttatc ggaagtcatc agtgcttctt tttgttattt tccgatcatt
     9541 ttgacaactg ataagagacc tctcgttgtt tttgtaaact ttgaatttga cgattatctt
     9601 tttgcccatg cactcgtact atcttagctg gcacacctac aactgtcacg tcttcaggaa
     9661 catctgataa cacaacagct gctgccccta ctttagcatt tgctccgatg tcaataggtc
     9721 caatcacttg ggcatgtgcc gaaattaagg caccttgtcg aaccgttgga tgacgcttgc
     9781 cacaatcttt tccggttcca cctagggtca ccccatgata aagcatcaca cctttttcaa
     9841 caattgctgt ctctccaata acaagaccag caccatggtc aatgaagact ccaggagcga
     9901 tttgtgctcc aggatggatt tcaatttgtg tccaaaatcg ccaaaattgg ctatgcattc
     9961 tggctagtaa tttaaaatga tgtcgccaaa gaaaatgaga caaacgatga gcagctaggg
    10021 ctttgatgcc tggataagtt aggataacct caagactatt acgagcagct gggtctagag
    10081 ctttaacaat agctatactt tctttccacc aacccatact aactcctacg acttatctaa
    10141 ttctgaccaa tcaacaagaa tttgttggtc atcaaaagga ttgatggcga caccgaacgt
    10201 ttcatcaagc tcgttttcac catttcttgg ttgataaata tcatcaatcg tccaagtcag
    10261 aatcactcct tcagctttcc tgaaaaggcc tccaaaatcg tcttggttgt accatttcgc
    10321 aaagctttga agatttgaaa aggcgggaac atagcttttt ccttcaggcg ttgacatggt
    10381 aggaaaaagg cggtcgtaat ggttattatc tttaggataa acaaaggcag gaacaaggta
    10441 aaccttttcc atcgtatctg ccgccacatt gtcatcactc ataagcgtat ttaacacagt
    10501 ggtgtaatgg ttcataaatt gaatcatatc actgcttttg aaaatggtag aattcccaaa
    10561 atctcccttt ttcttaaggt taaagataag acctgctgca ccagatgtaa tgacttcttc
    10621 tagcactgca agagctgaac gctccagcca atactgactt tgagcactct tttgttcttc
    10681 tttaaagcga gccatatctt ccttatcggt aaaaacaggg gtaaccttga caccttcgac
    10741 ttctatcacg tagggttctt tagcagccca gacaggaaaa ttatggaaag cgttgacaag
    10801 cgcaaggcta tccaaaaagt tatcaggcgc attgataaaa gctcttaagc gaatatctaa
    10861 ttcatttgat ttagtcatgt ttttcttctt ttttctctgg ttttggtggg cgtggaatca
    10921 aagctttcat tgaggcatcc acacgaccct tctcatcaat cttaatgacc ttaacatcaa
    10981 cgtcttcacc cacttcaagg acatctgaca cgttggtcgt tctcgtccag gcaatttcag
    11041 aaatgtgaac aagagcatct gtcttgtcaa agaggttgac aaaggcacca aatttctcaa
    11101 tacggacaac cttagcatgg taaacttcgc ctaccttagc ttcacgaaca agacctgcaa
    11161 taatttcttt agtgcggtca atggcagctt ggtcacttga gtagatagag acatttcctt
    11221 cgtcatcaat atcaattttg acacccgttt cggcaataat cttgtcaata gtttcgccac
    11281 ctttaccgat aaccactttg attttgtcta cgtcaatctt gatggtgtca attttcggtg
    11341 cagttggggc taactcagga cgtggttcag cgatagttgc ttcaatcaca tcaaggattt
    11401 caaagcgagc ttttttagct tgtgcaagag cttcttctag gatttgaggg gtaattcctg
    11461 caatcttaat atccatttga agggctgtaa tcccttcacg agttcctgcc actttaaagt
    11521 ccatatctcc aaagtggtct tcaagacctt ggatatcggt caagactgta taattcgtcc
    11581 catcggaaat gagacccatg gcaatccctg ccacaggcgc tttaatcggc acaccaccag
    11641 ccattagggc aagggtccca gcacagatag aagcctgaga agacgaaccg ttagattcta
    11701 gaacttcggc aactaaacga atagcataag gaaactcttc taagcttggc aagacttgtt
    11761 caagagcgcg ttcacctaag gcaccgtgtc caatttcacg acgaccagca gctccgtaac
    11821 gacccgtttc accaactgaa tattgcggga aattgtagtg atgcaaaaag cgttttttgt
    11881 actctggggc cagaccatca atgatttgag tttctcccat tggtgccaaa gtcaaaactg
    11941 acaaggcctg cgtttgaccg cgcgtaaaaa gacctgatcc atgtactttt ggcaagaagt
    12001 caaccacagc atcaagcgga cgaatttcgt caatcttacg accatcagga cgaatcttgt
    12061 cttctgtaat gaggcggcgc acctcagcat gctccatttg ttcaaggatt tctgccacgt
    12121 cacgcataat ggttgctagg ttttcgtctt ctgcgtaacg ctcttcgtat tcagccttaa
    12181 ccatttcttt aacggcttct gtggctgctt cacgcgcttt tttctcttca acttgaacag
    12241 ctttttgcag ttgagcattg tacttagcca caatatcagc ttgtaagtct acatcaacct
    12301 gtaacagttc aacctcagct ttttctttac caacaactgc tacgatttgt tcttggaagg
    12361 caattaattc ttgaatagct tggtgacctt ttaaaagagc ctcaagcatg atgtcttctg
    12421 ataattcttt ggcaccagac tcaaccatat tgatggcttc tttgctacca gcaacagtta
    12481 attctaagag tgatgcttcc atctgttctt tatctgggtt aatgatgaac tcgccatcaa
    12541 tatagccaac ttgaacacct gcaatcggtc cattaaatgg aatatctgag attgacaagg
    12601 ccagagatga gccaaacatc gctgccattg gagcgctagc attttcatca taagaaagga
    12661 cagtgttaat cacttgtact tcgttacgaa aaccttcggc aaacatggga cgaatcgggc
    12721 ggtcaatcaa gcgagctgtc aaggtcgcat ctgttgaagg gcgtccctca cgtttcatga
    12781 aacctcctgg gaatttacca gcggcataca ttttttcttc gtaattaact tgaaggggga
    12841 agaaatcacc cgttgccatt ttcttagaca taacagctgc ggtcaggaca gttgagtcgc
    12901 cataacgaac aacggttgcc ccattggctt gcttagcgac ttgaccaact tcaacaacaa
    12961 ggggtttccc tgcaaatgtt gttgtaaagg tttgttttga catatattct ccttttaatt
    13021 ttcagagggg taaagaggta cctgttctct gcttagattc agttgttata cgttgttttg
    13081 ctacaaaaag caacctgcca cgcttgtcac acgctcctct ttgtatcaaa cgacgtatct
    13141 gctacatttt accataaaat actagaaatt tcactgtcag atcgcaatct cttgaaatgt
    13201 ccttgaaaaa tattaaacga aacttatact ctttaacaaa gcaaaaaaaa gcaggttggt
    13261 atgcctgctt tcgtctccta atattaatga ttgtaatcct ctaaaatttt aaagccgagg
    13321 ctggtactgt ctttcagaaa catttgcacg gtttcaaggg aatagtcttc taaatctttt
    13381 ccttgaagag gcaacttggc aatcaaatca gtggtacaag tgataatgtc cacgcctagc
    13441 tgatcagctt gatagatatt ataagtttca cgaggactag cccacaacaa ttcgacccct
    13501 tctttttgac gacaaatgcg tagagcttct tccatgattg gcattgggtc aacgccggta
    13561 tctgcaatac gaccagcaaa aacagacaca atagctggaa ctcgagcggt taaatgatca
    13621 accacagctt gagtctgctc aattgtaaaa atggctgtga cgtttaactt aatgcctttg
    13681 gctgacaact tttgaatgag gggacaggtt gattctcctg ttgaagtggt gactggaatt
    13741 ttcacataga cattgtcacc tagactggca attttctcag cttccttttc catgcttgcg
    13801 agatcatccg caaagacttc aaaggacact gggtagtctg taattttggc aagcacttct
    13861 ttggcaaagc caatataaga actgatgcct gcttttttca tcaaacttgg attggttgta
    13921 aagcctgtga ccaaacctgt ttgtaaatcc tttaacatgg tctctaagac cgcaccgtct
    13981 gaaaagactt taacgtttag tttttttgtc atcatatcct ccttttagat ttctactgaa
    14041 acattattga tggcttcttg agattttgac agaaagtttg gaagcaccaa tacaatagca
    14101 agcacagcaa ccaccaagta aataccgatt tgaccaatcg acgttcctaa gcggcccagt
    14161 aaaatcccta aaacaccgaa atccacatct ccaaaggtcg tgtttgaaaa accaagttta
    14221 ccaagtacag gtagaagcat ggctggcaag aaggcgagta ataagccatt agcgaagcac
    14281 caatcatcgc accgcgacga ccacctgttg aatttccaaa aatctctgct gttgcaccgc
    14341 agaagaaatg aggcaccata cctgggataa tcaggacacc acctgcaact cctaaaatca
    14401 acatcccaag taagccacca acgaaacttg acgcaaaacc gatgataaca gcggttggcg
    14461 cataagggaa gaataccgca caatccacgg ctggaatagc atttggaatt aatttgttag
    14521 caataccttg gaaggctgga atcaagtcag ccaaaatcat gcgaacaccg gcgtagacaa
    14581 ttgccacacc aaccgcaaag gtaagaccac tcttaatggc aaaaataaat gggttttgac
    14641 ctgctgctaa ttcttctgct actgaagcat tcctcaaaac agaagccact gttgccacca
    14701 agtagaaaat caccatgata agtcctgttg aaatggtggt gttgcgcaag aaactccatt
    14761 tttcagaaat ctgaaggtct tcggtatctt tactgtcttt gccaaccttg ctaccaaccc
    14821 aggcagaaag gtaatagccc aaactaccaa agtgtcccat agcgatttca tctccgtcag
    14881 tcactttcag agtatactgt tgaccaatgg ctggcgaaat agctgaccaa gctcccaaga
    14941 ggaacccatc taagataatc aaaagacttc ctttgaaacc aacagctcca agcacggcgg
    15001 acaagagaca agccataaag aagctgtgat gtcctgttaa gaaaatgtat ttgaagcggg
    15061 taaaacgagc aaaagccaaa ttgagcaata acccaaccac caagatggac atagtttcca
    15121 cacccagaat cttttgagcg acagaagtga cggcttcgtt atttggcact acgccagtga
    15181 tgctaaaacc gtgctcaatg agtttggcaa gagggtccaa attggtcaca ataacacccg
    15241 cccctgcaac aagcataagg tatcccaaaa tcggccccaa ggttcctgtc aacaccttat
    15301 gagcaggtgt tttcaaagcc accaaccctg caaaggcaat caagcccatt aaaaatgcag
    15361 gttcttttaa aatatctcga ataaatgata ataatgcttc catagtatct tcctatttcc
    15421 tttaaaggct tattccttgc cactcatttc cttgagaacc ggaaccaaat cttcttcaat
    15481 ttttttacga ttggtatagc ttttaacgat acaaaccttg gcatcctctg gaaaaatatc
    15541 tttaaattct ttaacggtaa caaacaaatc tgcacccttt ccaacagccg cattggaatc
    15601 acaggattct gcatccacat caatacccaa actagaggcg atagcttcta ctttcatgcg
    15661 aagtaacaag ctactaccaa taccgtttcc acaaaccgtt acaattttaa tcatgtgatt
    15721 cgtctccttt ttcaatcata tggctaatca gactcattat ttcctcagta ttcttagcct
    15781 caattaattg ctcaatatgt tcatcatcat ctaaaatcaa cgacagttcc tgcaaagcct
    15841 tcaaatgtga actagaatcc acagcagaca aaacaaaaat caattgcact tgcttgtctg
    15901 gatcaccttc ttgcttcaaa tcaaagctga caggttcttt tagttgtaag agagacatgc
    15961 ctaactgccg cgttcctttt tcaggcgccg catgaggaac agccacctta ggggctaaaa
    16021 caatataggc cccaagctca ttgaccgaat caatcatgcc atcgatataa gatgtttcaa
    16081 tcatctggtg ttctagtaaa ggctgagcag ccaaacgaat cgcttcctgc caatccgaaa
    16141 cagcatcagc ctgcctaata aaatctgctg ttaacaagtc caatagtcct agacctcctt
    16201 tttcaatcgt taaatggtta ccaatcaggt aacgggataa atcactaaca agtccttctt
    16261 tattagtaat cgtggtatgt ttatgaataa tactcaacag atcatccaaa gcgaattgct
    16321 ctgacaaggg aagatgaaag tcatcacaga ccatcttttt caacatcatt ttttcaacag
    16381 gtcccatcaa agcttgcgtc acataaacag gtttagcaca gtcaaaagct actgttgaaa
    16441 aaatcaaatc aaaggatgct ggatccaaca actttatttt gtccagctgg tgaatcctaa
    16501 taaactgtag ctgtggaaaa agttccttca aggttgcctc caacatcaga gacgaactaa
    16561 tgccattagg gcaaacagat aaggctacca gctgattgct tggcctcttc ttaggggcct
    16621 gcaaccatct cccaaaatga atggtaaaat aagcaacctc atcttcatta actgatttcc
    16681 ccaattgttt ttctagagga gaaaggctgc gcttgaccaa gtaaaagaga gattcataat
    16741 cttgtttgat ttgttccttc aaaggattgg tcaagttaat gtcgtagtaa agtcggtaat
    16801 aagctggcaa aaggtgactg tataggttct gacgaagttc tggggtatcg gtgatagaca
    16861 atcccgtgtt aaccgccacc gaattgatga tttcctccat aatgtcataa ataggttgct
    16921 gaaagactaa ctctaagtca ccttggatac aaccaagtaa cctagattga accaaatatt
    16981 tttcttcggt tagactgggg aagttgacca ataactggtc caccatcgtt tcaacagctg
    17041 gcgaagctaa aggaaaagta tccactgctt cagcccttac gttccgtgaa aatggtcgat
    17101 gagcgagaag gcagaaaaaa taaatcaaat ccctcaattt ctccgaaata aaagttaagt
    17161 ggttctccct gctcagttcc tccaacgttg ctgccatcac ctttttctgg tcgggcaaac
    17221 caagttcatg caataagtaa ctgaacaccc aaggtccaga tgtgacttgc aaaagagaac
    17281 tgaccgctga ttctaagaga cgccgcaagg ctaagggttc tccttcaaaa aaataaccat
    17341 cctggcgatt gtaagctaaa gttacctgaa aagcttgtac ccgatcacgc accaagttta
    17401 aatcagcaat aacagaattt ctgctgagcc gtaacaagct ttccaagtgg ttaatggaga
    17461 taaattcttt agccatcatg atgtaaatga taatcatatc cagacgctca tcttgaaaga
    17521 gatagtcgtc taaggtaggt agcatgcgat tcaacaagtc cttttcagta aggtctggcg
    17581 gataaaccag ctgcctgtcc agagcaataa ggggtaactg atagagtgac aacgtgtcat
    17641 tgattttagc aatatctgcc agtatatgac gtttgcttgc ctttaaaatg gattcaaatg
    17701 cctccagtga atgtccttta tatttagaaa agagttgata gtttctgatg agctcatgag
    17761 atagcatgtc atctcctcct ataatgtcag tataaaatga agcgttttca ttttcaagat
    17821 aaattgagtc caattgtctc atgtcatttt tgactgcaat tggactcaac aggtgatatc
    17881 attgtaacaa gttcctcaaa aggtggtcaa aaataggcaa atctgggaat tgattccctt
    17941 tttcttcatg atacaacaaa agatacctca tcactgagat accttttgtt gttaaataaa
    18001 atcatcaact gctaaaaaaa cagtcttaga ccattctagc caaatgtgta agttaacaaa
    18061 caacatgtca aaaatgaaaa agcccactgc ataagcagag gactttttaa acttgaaatt
    18121 aacgacgaag tccaagagat tggatcaact cacggtaacg gttaacatct gtacgacgta
    18181 ggtatgccaa caagttacga cggtgaccaa tttttttcat caatccacgg taagtagcgt
    18241 ggtctttttt gtgttgtttg atgtggctgt tcaagtggtt gatttcccaa gtaagaactg
    18301 ctacttgaac ttcaactgaa cctgtatcgc cttcatgacg tgcatattga gcaatgattt
    18361 catttttttt ctcttttgag attgccataa tatattctcc tttttttgct taatccgagt
    18421 cttaggattg gcactcctaa aaccaagaaa aagttggttt gtctttcgac cactctattc
    18481 tagcagattc ataatattag gtcaaccgtt atcctattat tctaagctct cacatccaaa
    18541 ccaccttaag caacagaact agcaaggcca aatagccaag aggctgtttt taaatcagaa
    18601 taaacctgtg cgattgcttg cctaataaga taaccagcta aaagaggtaa gatggctgat
    18661 ccccgcactt cagctgataa gcggtaacct agcatagtca ttcatttctt tccaacaaag
    18721 tgttcactga taatattaat tgctctagta ttaacaaacc caatccctaa tccatgaaca
    18781 cagcttatta accgatgcga cctgccttta tgacataggt caagagttga cacaaaatgt
    18841 ttgccaacaa aacattacct ataccgtcaa agagcgcttt gcttcctata cccttgaagc
    18901 gcaagccaac caagaggttc atctaaacct tacattatta gcaaaccgtt ttggaacaag
    18961 cgatcgccat cttaagcacg tacttaagca gcccattttt cagaggatta tcgaacgcat
    19021 ctgcttcaaa acctatcgta ttgccgattt agacgctcta actcaattaa gacctctgag
    19081 gtatgcagcc cattaactta taatagcaat cactctaacc actatctgac ttttctttaa
    19141 aaaagaaatg atttgtgata tagtaaatcc agttaaaaaa aagtaaagga gtagacatgt
    19201 ctgcacaaga taaattaatt aaacctagtc atctgattac catggatgac attattcgtg
    19261 aagggaatcc aactttaaga gctgtcgcca aagaagtgag cttacccttg tgtgatgagg
    19321 atatcctatt aggagaaaag atgatgcaat ttttgaagca ttctcaagat cctgtaatgg
    19381 cggaaaaact tggtcttagg gccggtgttg gtctagcagc tcctcagatt gatgtgtcaa
    19441 aacgtatcat cgctgtgctc gttcctaatc ttcctgataa agaaggaaat cctcctaaag
    19501 aagcctatag ctggcaagaa gtcctgtata atcctaaaat cgtttctcat tctgttcaag
    19561 atgcagccct ttctgatggc gaaggttgtt tatctgtcga tcgtgttgtt gaagggtatg
    19621 tggttcgcca tgctcgtgtt acagtagact actatgataa agaaggacaa cagcatcgca
    19681 tcaaattaaa gggatacaac gctattgtgg ttcaacatga aattgaccat atcaatggtg
    19741 ttttgttcta cgatcggatc aatgctaaaa acccctttga aaccaaagaa gagctcttga
    19801 ttttagacta acaaaaaaag gccctttagc ctttttttgt tagtctaaat agccgctaaa
    19861 agagcctctg cctgagatag caagtttgct tttgtctctt ttgaaatctc aagccttcct
    19921 gttccccagg catcaggatt cacagttgct tttgtaaact ctcctgctac tgaagttcgg
    19981 ataaacggca atagtgcttt aaactgatca aatacttgat catgcccgcc atttgcaact
    20041 gaagagaccg taaccacctt accgccaata gcagatgggc ccgtcggatc agacaaatca
    20101 agagcacgag acaaccagtc tagcaggttt ttaacagaac ctggaataga gaagttgtaa
    20161 actggtgtaa aaatccagat agcatccgct gactgaacag cttgacgagc gtcaacaact
    20221 ggtaaaggtg cattagcttc gatatcttga ttcaaaacag gaacgtcttt ccaatttaag
    20281 taagatacaa ctgcttgatg ttccagagct ttttgtgctt gagccgctaa ttgatggtta
    20341 aaagaccctt cacgaagcga gccaacaata aataaaatat gtttcatctc tttgccctct
    20401 ttttttaaag atcgttttaa agctttctaa aagtggtatt tcattgatac ttctttttga
    20461 aatttatcac tatgtagtaa ttatatcttc tctaagaaga gctgtaaaga gatttgttta
    20521 taattgtttg aactttttga gaagttcaat cagacaaagt tgttcttttt cagtcaaaac
    20581 agcaaaggct tcttccaccc gtgcgacgtg tttaggcaaa acagcttcaa tcaagcgagt
    20641 acccttatct gttaaggtaa ccacataggc tcttttatcc gttttgtctt tgcacttgct
    20701 aatccagcca ttgcgttcca tattatttag tacaacagtc atattccccg aagtcgctaa
    20761 caaagaatca atcaagtgat tgattcgcat acagccttta gtatatagta cttcaagcac
    20821 actgaactgc gtggctgtta aatctgcctt tttaaaaata tcagccccaa aagcatctag
    20881 cgttcgctga gctttacgaa acaccaccat ggctttcaaa gcggtgttct tatctaaatg
    20941 actcatttga tttcctcgct ttatacggta tgtcacagtt tagtatcaga gagattagaa
    21001 agtgatcttg tccctcttct tttatgactt tgcatattac tatcatcatg ataatctaga
    21061 aaaagtcatc aaaaagactt aattgattat cttctggcat atttcctaaa atacccatct
    21121 catccatttt ctcaacgagc gttgacgatg ccccgcctcg tttacgcaat tccattttag
    21181 agaggaattc gccttcttga cgagctttaa cgatttgctt ggccacgttt tcacccagac
    21241 cttctagcgc tataaatgga gggataaggg tatctccttt gatttggaat tctatagcat
    21301 cacttttgta aaggtctaat ttgccaaact taaagccgcg ttctaacatt tcgttgacaa
    21361 tctccaaggt tgtaaagagg tcattttcca cattggtggc ttcattattt ttacgtttta
    21421 tagtaatatc ttccattctt gctttaacag catctaaacc accactcatg gtttttaatt
    21481 caaaagcctt cgcacgaata gagaaataag cacaataata cataatgggg tggtgcacct
    21541 tgaaataagc cacccgaagg gccatcaaaa cataagctgc cgcatgggct ttagggaaca
    21601 tgtacttgat ttttccacac gattcaatgt accagtcggg cacattgttt tctcgcatgg
    21661 catcaatata gccattacgt tcttcctcag aaatttttag ccataatccc ttacgcacac
    21721 gctccataat ggtaaaggcc atttttggtt ctaagcctgc gtgcatgagg taaaccatga
    21781 tgtcgtcacg acaaccgata acggttttta gggttgcaat gccttcttta atcaaatctt
    21841 gtgcattacc aagccaaaca tcggttccat gagatagtcc agacaactgc aaaagctccg
    21901 caaaagtggt cggatgcgtc tcattaacca tgccgcgaac aaagttggtt ccaaattctg
    21961 gaatgcctag cataccagtc ggtgtcccaa tttgttccgg ggtaacgccc aaaacctctg
    22021 tcccagaaaa gagagccata actcccggat catcagcagg aatagtaata ggatcaatgc
    22081 ccgataaatc ctgaagttta cgaatcatgg tcggatcatc atgccctagg atatcaagtt
    22141 tcaagacgtt ttcatcaata tcatggaagt taaagtgagt tgtctgccaa gaagccgtta
    22201 catcatcggc tggatattgc acgggggtaa aatcataaac atccatgtaa ttaggaataa
    22261 caacaatccc cccagggtgc tgcccagtcg ttcgtttcac accagcagca cctgctgcta
    22321 gacgatccac ctcagcatca cgatagaact tgccatagtc gcgttcatag cctttgacaa
    22381 atccataagc tgttttttct gctacggtac caactgttcc agcacgaaag gcgtattcgt
    22441 caccaaaaat atctcggaca tccaaatggg cactgggctg gtcatcacca gagaagttca
    22501 aatcaatatc gggcacctta tccccatcaa acccaagaaa ggtctcaaag ggaatgtctt
    22561 gcccatcttt ttgataaggg gtgccacatt tcggacaggg tttattaggc aaatcatagc
    22621 cagatccaac tgacccatct gtgataaatt cagaatgttg gcaggacggg caaacgtagt
    22681 gaggcggcat aggattaacc tcagtaatcc caatcatggt ggcgacaaag ctagacccta
    22741 cagatcccct agaaccaact aggtagcctc gctcatttga ccggttaaca agcatttggg
    22801 aagcgagata aatcacagca aaaccgttcc ccaagataga ggttaactct ttttcaatgc
    22861 gtaaatcaat aatatctggg agaggattac cataaatttc aaaggctttt tgataggtta
    22921 attcggcaac cgtctcttcg gccttatcaa tatacggggt gtaaagatcg cccttaacca
    22981 cttccacttc ctcaatacgg tccgcaaaat cctgagtatt ttgcacaact acttgataag
    23041 cgaggtcttt tccaagaaag gcaaactcat ccagcatttc attggttgtt ctaaagtgcg
    23101 ctttaggtag aggagcaggc tgtgcccctt ccccacggcc gattgttcta ttaatcatgg
    23161 caccctgacc aagactacgc acaataattt cacggtaaat ctcttcttca ggctctagat
    23221 aatgcacatt cccagtggca agcacaggtt tcttagctcg tttccctact tcaatgaggt
    23281 cacgaatcac ctgctcaata cctgcttgat ctttgattaa ttcacggaca accaatggct
    23341 ggtaaatggc tggtggcatg atttcgataa aatcataata cctagccaaa tcaaccgctg
    23401 catcaattcc tttagtcaga acggcatcaa aaacctcgcc gtcagaacaa gctgtaccaa
    23461 gtaacaaacc ctctctgtga gcatctaaga cggttcttgg aatacgcggc accccttcaa
    23521 aatatttgat attggaaagg ctgaccaact taaacatatt tttaagacca acctgatttt
    23581 gcacatagat agtcgcatgc ttaatccgcg cttttttgta agaatcctca gccaccaaat
    23641 ctgtattgag ttgcaaaaga tttttgatgc catgcttttc tctggcatct tttagaaaaa
    23701 taaacaaaag acgtcctgtg gcttccgcgt cgtaattggc catatgatgg tggtctagac
    23761 tcacttggaa acgcttggtg agcggtccca aaccgtgacg cttgtactca ggatacaagt
    23821 ttctagcaaa ttctaaggta tcaatcacag gctgtgtgat tttgggcaag tcgtggcgtt
    23881 cataattggc gttcataaag cccacgtcaa aactggcgtt gtgggcaacc aagatactat
    23941 ctttgcaaaa gtcctgaaaa gcttttagga cagtaaccaa tggcttggcg ccctgcaaat
    24001 gcttatcggt aattcctgtc aattcggtgg taaaggctga aagaggatgc ccaggatcaa
    24061 tgaattcatc aaactgctct acaatatttc ctttaaacat tttggaagcc gcaatctgaa
    24121 tcaggtcatt attcatagca gatagacctg tggtttccac gtcaaagacc acataggtag
    24181 cttcgtgcaa atccatatca acaggttcat aagaaatagg caccttgtcc tcaacaatat
    24241 tggcttctag gccaaaaata gccttaatcc cagctttgcg agccctatgg tagccatgag
    24301 gaaaactttg cacattagca tggtcggtga tagcaatcgc cttgtgtccc cacttggctg
    24361 ccgtatcaat caagctttct actgtcggta aggcatccat ggtagacata ttggtgtggg
    24421 catgaagttc gacccgcttt tgcccttctg gcatcaggtc tttgcgctca tgacggacaa
    24481 tttctttgac ctgctggaca ttcatggtga gactcttcgt aaaaggattg gtctcaatat
    24541 tcccttgtac ccgtaaccaa gctcccttag caatcatatc aaatttacgg agctcctcat
    24601 cgtctttagc ccatttttgg agagcaaacg aggaggtata gtctgtcatt ttaaagttga
    24661 tgatatggcg acctgtccta gtcgttttac gctccacgtc aaaaaccata ccctcaaaga
    24721 caatccggtt ttcttcggtc tcaatctcaa tcattggtgt gatggttgct ttttcaaacc
    24781 ctgcctgacg cttagctgct cgttccttgt agtcaaactt aggagcaggt gtggcttcct
    24841 caactggtgg catcatggct tcaagagatt tttgggcttc caaattatcc tgcacagcct
    24901 ttttcacaag agcctgacga ctggaaacaa aattcttggt caaatgctca gtcatttcct
    24961 gatctgacac catatctatg gtcaaggtgc caaagccaaa ggcttctaat tgcttgacca
    25021 gattaggcag atgattgttt ctaaaatgat cgttattcac aaaacctggc gctgcaataa
    25081 tgagtttgtc atcctcataa gtcactttga gctttgagaa agaagattta aaactagcac
    25141 tattacacgg tgcatgctca aaagcttctt ggtaataagc ttgaagcaga tcatctgaat
    25201 aatccacctg agcagcttgg atgtcaaagg ttaccttaat gtcagccgcc tcaaaagttc
    25261 ttatcaaacg atcatgcaat tcacgataag ttgcaatcgg taaaaccgct gcaaaggcaa
    25321 aatgaaattc ccacaagcgt gacaccgaat gtacctttac ctcgataata tcagcagatg
    25381 aaaaggcact tgaacgtctc atgtcaagtg gcatttctat ctggtccatc aatttagcga
    25441 ataaatctga cataattttc tcctgatttt actaggcttt attttatcac aattcaagat
    25501 gattttcgag ttttagatgg aaagaaaagc agggctttct tctgacgttc tttggctaat
    25561 ttattccaag tgatagctca gttgaggtca ttttttttgc caatctttta tcgccaagcg
    25621 cttttggtaa agcaaaagcc actctgtatc agacaagttc acagcaaaaa gaggccactc
    25681 taaacaggca gcctcttcat ttctttttag tgttcttttg ttaaaatttc aagggtttcg
    25741 atgaggtttt ctgcattaac ttcaatgctg tcacctgttg ccttgatttt aatttccacg
    25801 ataccttcag cggctttttt accaacagtg acacgaattg gcaaaccaat caaatcgcta
    25861 tcagagaatt tcgaaccgac gcgttcatta cggtcatctg tcaagacatc atacccttta
    25921 gccatcaagt cagcttctaa cttcgccgtc aagtcttggg ccacttgatc tttgacatta
    25981 actgtaatca aatgcacgtc aaatggggct aattctttag ggaagttaat accccaagca
    26041 taacggtaat cgccttttgg tgtcttgttc acaaagagac gggcatgctg ttcaatgaca
    26101 gcagacaaaa tgcggctaac cccgatacca taacaaccca tcacaattgg aactgttctg
    26161 ccattttcat caagaatcgt tgctcccatg ctgtctgaat aacgagtgcc caacttgaag
    26221 atatgaccga cttcgatacc acgcgcaaac tggagaacac catgaccgtc cggagacatt
    26281 tccccttctt tgacttcacg aatatctaca tattcagcct ggaagtcacg tcctggattg
    26341 actcctgtca tatggaaacc atccttgtta gctcctgcaa cggcattggt aaggttttgc
    26401 actttgcggt cagccacaat acgactacct tgtgccaagt tgacaggccc aagtgagcca
    26461 aaacctgcac caaagaaagc acgggcttct tcttcactag ctggctctaa aaaatcagca
    26521 gctagatagt tttttaattt aacggtattg atatggtcat ttccaactag caaagcaaca
    26581 acaggttcat tatctgccac aaaaagcaag gttttgatgg tttgtgtttc atctactgaa
    26641 agaaaagcag ccacttcatc aatcgttttg caatgtggtg tctcaacttc tgccaaggca
    26701 tcttcagcag ctacttttga ggacggtttg tattcgttag ttgccatctc aaggttggca
    26761 gcgtagcttg attctgttga ataagcaata gtatcttcac ctgaaatcat ccaagcagcc
    26821 aattctgcct taatatcttc taagacctct tttggaatat catccattga cgcaatagac
    26881 ttgtcaagaa ccacccagcg atcaagatcc gtacgagctg gtgtaatggc cataaattct
    26941 tgggaatctt tcccccccat ggcaccgcca tctccaataa tccctttgaa atctaaacca
    27001 gctctggtaa aaatagcttc gtaagcttga cgataatctt cgtaggtcac atctaaatct
    27061 tcatagttgt gatggaaact atagccgtct ttcataataa actcacgggt acgaagcaag
    27121 ccattacgcg gacgtttttc atcacgatac ttagactgaa tttgataaag gtttaagggc
    27181 aattgcttgt aagatttgac cgcatcacgc accaaagtcg taaaggtttc ttcgtgagtt
    27241 ggacccaaga taaagtctga gttatcacgg tttttaagct tatagaggtc ctctccatag
    27301 gtctcataac ggcctgattc acgccagaga tcagctgtca aaagagctgg cgccaacatt
    27361 tcaacagcac cgatcttttc aaactcttca cgcatgatgg tcttgaattt ctcaatggta
    27421 cgatttgcca gtggtaaata agcatagata ccagcagaaa cttggcgcac ataaccggca
    27481 cgcaccataa gcgcgtggct gataacctgg gcatcacttg gcatttcgcg caaggttggg
    27541 ataagcagtt tactttgttt catagattat tccgttttaa tacatcccct tatcctcaaa
    27601 cccttctgga aacatcaagt aagagtgaag cgggattatt caataatttg aatgtgtatt
    27661 cctttcattt tgagtaagac ctcaaccctt gcaaatctta gggttgaggt ctttttcgat
    27721 ctttaacatg ataaggtcag cttaagatga cctatcttga ttagaaaaag acgcgcatga
    27781 tatcattcca tgtcacagca atcatcaata cgaccatgat agcaacccca gctagggtga
    27841 tataggcctc tgtttcttgc ttgataggct tgcgacgaat ggcttcaatg atattcatca
    27901 agatttttcc cccatcaagt gcaggaatcg gaatcaggtt aaagatccct aaattgatcg
    27961 aaagcattgc cataagagat aagactgact ctaagccgtt ttgagcagcc tgattggaca
    28021 tgtcatacat ggcaacaggt ccacctaatt tattgagact aaagccagtg atcaatcctt
    28081 tcaaagcatt caaaatagta aacgccctac tccaagctaa ttctagaccg cctaagagct
    28141 tatccttaaa tcctgttttc agactcgcct taactcctat cgtgtatgtc tttgcatgct
    28201 tttgtggttt cacagccacc gtttttaagc gctgatgtga cttgtaggtg accttaatgg
    28261 tctgtgacgg gcctagatcc cgtgttgcaa ggtctacagc ctcagtgaga tcattccagc
    28321 tagtcacctt gtaaccatta attgcgacaa tttggtcatt atctcgaaga ccagccttag
    28381 ctgctgctcc attttcttga acacggacat gattgctact aaaatctggc atcccacctt
    28441 gtaaaaagac caagaggata aaaaccacaa tccctaaaat aaaattattc atgggacctg
    28501 caaaattggt gattaaacgt ccgccaatac tagcattttg atattgaaca tcaagcggag
    28561 caattcttat ctcagtaccg tcttcttcaa caatagtagc atcgtgagcg accttatatg
    28621 tcttggtttc ttctaaaact aagccagtaa tactcagctg atcttctaag tcatagcctg
    28681 taacatgcat tgggagactc gttgggtcta acttactttg agacaagttg atgcgcttaa
    28741 caaaaccttg ctcattaagg gttaaacttg ttggagtacc tgttttgatc tcagttttgt
    28801 catcacccca accagccatt cgcacataac cgcccaaggg taacatcctc aaggtataaa
    28861 gagttcctcc ttggtcaaca tgagaaaaaa ttttagggcc cattccaatg gcaaattctc
    28921 ttactagaat gcctgatttt ttagcaaaat agaaatgtcc aaattcatgg acaatcacta
    28981 aaataccaaa aataataata aaggttatta ttcctaacat agatttcctt tcagtctatt
    29041 gcttttttga gttttgtcgc gttgttgcga ctccaaacat tacctcttaa gattaaaata
    29101 aaccaaagag atgcatgatg ggaaaaacaa aaatcatgga atcaaagcgg tctaaaatac
    29161 caccatgtcc cgggataagc ttacctgaat ctttgacacc aaagtgacgt ttgagagcac
    29221 tttcaaccaa atcaccaaat tgggcaaaaa tggaaaaaag agcgaccaaa accaacatgg
    29281 tcaaaaagtg atgtggcgca taaacagacc tatcaatcac cataaagata aaagacacaa
    29341 ggactgcaca agcgatccct cctaaactac cttcaatggt tttgtttgga gaaactgtag
    29401 gcaatagttt gcgtctacca aactgacgcc caatcagata tgctccaata tcagtggccc
    29461 aaacgataaa taaggccaaa aagaccttgt caattcctga caggcgagca ttgatcaaat
    29521 gttgaaaacc aatcccaaca taaaaactag ttgcaatcgg aaaggccgca tcatcaaatg
    29581 aataagctct gctatttaaa acagtacctg ccaaaatgaa aaaaaccatt aagctgtaaa
    29641 aagcaacatt cgcatcaatt ggtaaaaaag tgaggtaatg gtccatcgga actgccaata
    29701 caaaggcggc taacatggca aaaacacctt caaaagaaaa gacttctaat cttttcattt
    29761 tcagtaattc tgatacccca atcatggcca aaacgcctac aaataattgg aatggtaggt
    29821 ttcctattat gagaaaaggt aggaaaatag ctactgctac tcccccccaa acaacacgtt
    29881 ctttcataac tatctccttt tagaccttgc caaaacgacg ttggcggcgg ttataatccg
    29941 caatggcctt tagtagctct gcctttttaa aatcaggcca caagactggt gtgaaataaa
    30001 attcactgta agctgattgc caaggcaaga aattgctcaa acgcagctca ccactggtgc
    30061 gaataatcaa atcggggtct cggtagagat atggcagatg gtctgtcatc aagtaattag
    30121 caatcaaatc ctccgtaata tctcctggat ttaatttggc atctaagaca tcttgagcaa
    30181 taaagcgaac agcactggta atctcagcac gtcctccata attgagagca aaattcaaaa
    30241 ttaaacccgt attacgtttt gttttgtcga tggctgcatt tagcgctgcc aaggtatctt
    30301 caggtaaacg actagtctct ccaatcattt gaatctttac attgttctca tgaagcacag
    30361 gcacatattt gtcaaagaag gttactggta aattcatgat aaaagacacc tcatcttggg
    30421 gtctggacca gttttctgtc gaaaaggcat agactgtcag aactttaacc cccaactcag
    30481 atgcagtaat cgttacttct tgtaaagcat ccatcccagc tttatgccca aacactctag
    30541 gttttagtct ttttttggcc caacgtccat taccatccat gataattcca atatgctttg
    30601 ggatactccc caaaactttt tttgttgatt tcgcttttaa tccaaacatg ttttttcctt
    30661 attgataagc atacttatct catattttat catatttcac gccttttggc ttgtttttta
    30721 tcaattagat cttatcttca caaaatgttc acctatcctc tgatagatgt gtcagctttt
    30781 catgaacctg ttagcatcag aataactgtt tcaatccagg gatcaaaaca aaaaagtttg
    30841 gcatatttca tccaaacttt ccccgcctta atggctttca atggcactgt cagtgtcagt
    30901 gctatctact gtctcaacag caggctcatt aacaacactg gtttcttctg tttcaaccag
    30961 tgtcgtttct gtagtagctt ttgttacaat acgcttaata gctaatagct caaatgtcaa
    31021 aaagacgcca tccacatcta acacaatctt tttagctgtt gtatcgactt cgtcaacaat
    31081 cgcaaacatg ccaccaatcg ttacgacttc gtctcctttt tcaatagcgt ttaactgatt
    31141 ttggcgttct tgcgcttgtt ttttttgttg acgttgcata aaccaaatca atccaagcat
    31201 cactacaaac attaaaattg ttggaaatcc cataatgtct cctttttatt ctataaaagt
    31261 catgtcttac tatatcaaaa atcaagcggt atagcaagac tagacgttac ctgatgtctt
    31321 ctttaaacga gacgctaagc gcagtgcttt actcacgagt caaaaaagct aagatgtcat
    31381 ctggtgtcaa acttgaatca catacagccc tttggtgctg atcgtaacta accagcaaag
    31441 ctggaactgt cactaattgg tagttctcac gaaaggcagc aagctcagca gcatcagcag
    31501 cgttttcgct atccacaaaa tagacttctt tttgattatc tgttgctact tgtgccaact
    31561 tgggagcaaa gcggcgacaa taaggacagc tcgaacgtcc taaaaagaca atcatgtctt
    31621 tgccactagc gatagctgat gtgacttctg caacactcga cggtataaaa ttcgcaacga
    31681 tttcttcaaa tgtcatggat gtctccttct cttttggtct catgatacac taaagtagca
    31741 attttctcaa tcacaaggtc ttttcacagc tcagagcttt tatagccgtc aaaaagaatc
    31801 cgcaaacagc aatctttttg tctctattaa caccacccta atctttttgg cgcttcatca
    31861 acaggctaat agccaataaa agcagagcta ttccagctgc taataagcct tttgaggagg
    31921 cttcacccgt ttttgggagc ttttggtttg ttttagctct ttttggtcta gtcattaatg
    31981 atttgtctag gtcttttgag ccatctggtg ttttggtttg atgattgtct gtctgactct
    32041 tttgctgaga gggattagcc gtttttgagg atactttgag gaccagggca gttaggcctt
    32101 caatagtcaa gccttctttg gtaaactgga ctcccttagg cttagcaatg gcagtaacac
    32161 cagcttgctc agcatcaaca agcacttggg ctcctagcaa atagcggtag gcttgaggta
    32221 aaactacctt gcgggtcttg ttgtctgcat tgacaaagac agcatagcga tctccatttg
    32281 atgccactgt ttggtaaccc atgatgaggt cctcttgttg aataccatct tgtcctgctt
    32341 gggtgatcaa ggtcacatcc cgatctacct cagctttggt tgcttttgta aaggcatctg
    32401 ttgagcgacg caacgcaatt agtccctgtg tataggcttt tgtttggttg ctaatcgggt
    32461 gagctatgga atctgttgcc tttgcccagt caaaatgatt gaccgcatca gacgaatcat
    32521 aagaatcgtg gatgaagtaa gggtattgcg ctacagcatc aatcagagtc gccttatttg
    32581 gcaccttgtc atcagacgcc tttgtcttgt agtcgggatt tagaagctgc ttggttcgtc
    32641 catattcctg accagaatgg ataaaggcag tcccttgagc agttaaaatc atggtatttc
    32701 ctagacgaat acgcttgtga atctcttctt cagccacttt aggatcctta ttgatggatt
    32761 tggcaatgac atcatgtaag gtcaggttgt catgggctgc aatatactgc actacatctc
    32821 ctggggcatc tgcttcaaag ttaccaggct gtgctttgat cgttttgaat aaaccttcta
    32881 gattttttgc gccaccagta ataaaggctg ctgtgccttc atttggaaaa cctgacttga
    32941 gggtatttct gatatcatca gagaaaacac cgaccgtatt ggttgctttc atccaatctt
    33001 gatctgccgc aatttctttt ttcccctcat caccttggta ggtacgccag ccttcgccaa
    33061 tcataatggt atttggatta atggctttgg ctgccttaaa ggcttgctca atagctgccg
    33121 catcatggtc acccatcatg tcgaaacgaa aaccatctac cttgaattca cgagtcagat
    33181 aagtaatcga atccaccaag atacgacgac tcatggcatg tgtcgttcct agacgacctc
    33241 cgccaaaact ctctctagct gtaccatcag cattcataaa atgatagtag ttgggttcca
    33301 aatcttcaaa gagataggtt ctagccgtgt ggttataaac cacatcaaaa ataacaccca
    33361 taccacgttt gtgaatctca ttgacaaggt ttttaagctc tgcgatacgt aaagcagggt
    33421 cattaggatt tgccgaatac atgccagaaa gggcaaagta gtgttgtggg tcataacccc
    33481 agttgtaatt attgtctgaa gacgtgtagg ctgttgagcg gctcttgtcc agctcattgg
    33541 cataaaaata actcaaaacc ggtagcaatt gaacgtgggt aacccccaag tctttgagat
    33601 agtctagctg ttcaacgaaa gctgaaaaag tcccaaaagg gtgtgttaac ttgccttcta
    33661 gagccttatc tgacgtaaaa tctcgcacat gtgcttcata gataatagcg tcttcacgct
    33721 ttttaaagtt gttaattttg gcaaaatcaa ggcctgttgg tcctagtttg cttggatcaa
    33781 taaaggcagc ttttgctgtt ttgatgtcat cagtagcagt cgcatcattc caggcagcga
    33841 gagatttggc gtaaggatcc aaaaccatga ctttttcctg accgcgcgtg atttcataaa
    33901 ggtaatagta gcctgtgtaa tcactaatgc ccttgacact gtcagaagtt agatgagctc
    33961 tccaaacacc cttgtccgac ttggtcaaat cagcttgacc aaccaccctt gtctgatctt
    34021 gtttatcata aacgacaacc ttaacagtat cagcacttgg agaccatagc gctaaatcaa
    34081 cagaaccatc cttagctagg gtagctccaa gttcaccatc gtaagcatag agtttatctt
    34141 ttaattgcca ggattggcga gcgacttggc taacttctcc aaatgtaacc gtgtagactg
    34201 caccttgcgc cttaaaatca ccctttaatg tcattacaga cttatccctg tcaagtgtca
    34261 actcatcaat tgcaacagtt ttccctgctt tgtcagtgat cttgatgttt tgtttcaccg
    34321 catcttcatc aagcccatct aaggtcgtaa aaatggcttt aatctcattt ggcgtggttt
    34381 gttcagctcc tttgagacta acctgatcaa tataataagg attgttgtaa acttttgggt
    34441 cagtgtcttt gacaaaaacc tgagtatgat tgtctaactc tttaaataga taatcttttg
    34501 gttgcacttt aatagcatct cctgtcttac ttttatcaag gattaaaaat ccgatttcgt
    34561 tagctccttc ttttaagggg acatcaacat aagctccata atgcccttta tgtgacaagt
    34621 caagtccatt tggccagtcg gtcgttgggg ttttgacatc tttaaaggtc cagacagcta
    34681 agttatcgta gtgtcccgat tgattgtggt agttgattcg aaggtaacct tttttcaaag
    34741 gtcgataagc gtgcgcatgg taattctcgt ctatccaaac ttcattcatt tttggcgtga
    34801 gaagcgagat gtgctggtcc tttgaaagat tctctccagc tttattatta atgagataag
    34861 acacttgctg gcggtgttta gctgctagtg gcacatctag ataatagcca tagtcatctt
    34921 ttttcgcttt tgtcatggtg atagcaccat ttggccaatc ctttgaaggt tgatccacat
    34981 ctccccacac ccaaagtccc aagcttccta gcgactcacc agctggcaat gttttaaaat
    35041 gcatacgcaa atgatgctca gcaatactag caggattagc tgacgagctg tctaaaactg
    35101 gttttgcctc cttgctagac gattgaagac catcaccaga tttagcattg ccatctcctt
    35161 gttgagcttc atcgcgggct agtccaacaa tggttttagc agcaacttga tgtgttaaga
    35221 tactaggggt gcaccctaac acgacagtcc cagttgcagc gattacaaaa cctatccccc
    35281 acttttttaa cagatattga tagcgctttg atccttggtt gactttcttt ttcataagac
    35341 ctccatttag tataacattc aaacaagact atcttatcac attgcgcaag cgcttgcaag
    35401 acatgccgca cttcaactta gaacacagct caaacaatga ctatcacccc ttaataggat
    35461 ctcaaaaatt aaacagccaa aacgagtttc cactcgcttg gctgttgggt aaactcaaat
    35521 cacatacagc tgctaggcct tcagtttgag acaaaaggca tcccaaggtt gtaagtgctt
    35581 ggtctctaat accgtgtcca catctgtatt ggcgatgatc acttgtgcac ctgctaagtc
    35641 tgtggcaaag acttgctcct catcagacac attgacaaca atcacatacg tctcttctcc
    35701 aagctgacgc tgataagcaa acactttatc agctgttggc aagaggtgat aatccgcttc
    35761 aactagccag tcttgctcct tacgcaaggc aatcaatttt tggtaggtgt aaaaaataga
    35821 gtctggatca gcaagggcat cggccacatt gatgtcttgg tagttggggt tgaccggcaa
    35881 ccaagtctct tttgcttcag aaaagccagc atgtgtgtcc ttagaccact gcatcggcgt
    35941 tctagcattg tctcgtccta ccttacggat gctatccatc acacgcgcag ctgacactcc
    36001 atttcccata gcttccttag catagttaag ggactcaata tcattaacct cggttaagtc
    36061 tttaaaagga taattggtca tgccaatctc ttctccttgg taaatgtaag gggttcctcg
    36121 catcaaatgc agtaaaatag ctaaagcctt ggctgatttt tctcggtaag tgctgtcatt
    36181 gccccaaatg gataagaccc gtggcaagtc atggttattc caaaaaagcg aattccagcc
    36241 ttctcctaac tttaattctg tctgccactt gctaaaaata gctttcagag caggcacgtc
    36301 taattctttg gcataatccc acttgggagc gtcaggttta tgttgaagac ccacatgctc
    36361 aaattggaag accatggata actctttgtt ttcagggcga gaatactggc gagcaatttc
    36421 aggcgttgct ccccaagtct caccgactgt catcacgtca tggttgccaa aggttgcttg
    36481 attcatttct ttgaggtaat cgtgtaagcg cggcccgtta cccgtgatct ccaagtcagg
    36541 aaccttccca atcaggtcaa tcacatccat acgaaaacca ccgatgcctt tggcaatcca
    36601 gaaattcatc atatcgtaaa ttttttggcg cagctgagca ttttcccagt tcaaatcagg
    36661 ctgtttcttg ctaaacaaat gcaggtaata ttgtcctgat gcctcatcca attcccaagc
    36721 agatcctgaa aaaatagaca tgaggttgtt tggctcgtca cgccaaatat agtaatcacg
    36781 ctcaggacta ttgggatttt ctctagcttc aacaaaccaa gcatgctcat cagacgtatg
    36841 gttgaccacc aagtccatga taatcttaat gcctcgctca ttagctgctg ctaagagctc
    36901 atccatatct gccatgtctc caaaaacatc agcaatcgct tcatagtcag agatgtcata
    36961 accattgtca tccattggac tttggtaaac aggagataac caaatggctg taatgccaag
    37021 tttttgcaag tagtccagtt gactggtaat gccttttaaa tcaccaatac cattgccgct
    37081 ggtgtcttta aaggaccttg ggtaaatctg ataaatcgta gctttatgcc accagtgttt
    37141 cttcattatt cttctcctat taatcattca ttcatctttt attttaacgt tttttctctt
    37201 tttgtgcaat cgtttgcgca aataacatga taaaaaggct ggaacccaac ctttttatga
    37261 atatcgctta taccttaacg aatagcttgt tcggtatctc tgtcaaagaa atgcccctta
    37321 gccacattaa aggtcaagct aacttgctcc cctggactat ggaaatcacg cgcatctaca
    37381 cgagaagcaa actcagtttg tccaagcttg acatagagca tcgtctcaga gcccagtaac
    37441 tcagaaacca aaacttctgc tgtgacagtt gcacttggat aagtatcatg cacgatttgt
    37501 ctactagaaa tgtcttctgg acgaatacca aaagtgactt tttccccctt gtaaccagca
    37561 gcttcaagca tttttgcttg tccttctgga atagcaatgt caagaccatc ttcactcaca
    37621 atgcggccat ctttaacttc cacttcaaaa aagttcatgg cagggcttcc gataaaaccg
    37681 gcaacgaatt tattagctgg aaggttgtac aattcttgtg gcgagccgac ttgctcaatt
    37741 ttaccaatcg ttccattacc ttgagggttt ttagtggcac tcatgataac gatacggtct
    37801 gccaaagtca tggcttcggt ttggtcatgg gtaacgtaaa tagttgttga gccgatacga
    37861 cggtgaattt tagcaatttc agcacgcata gacacacgaa gcttcgcatc caagtttgac
    37921 aaaggctcat ccatcaagaa taccttagca tcacggacaa tagcacgccc catggcaaca
    37981 cgctgacgtt gaccaccaga caggtcagcc ggtttacgct ctaaaaattc ggtcaaacca
    38041 agaatctgcg cagcttcctt gacacgacgg tcaatatcat cttttttgta cttacgtaat
    38101 ttcaaaccaa aggccatgtt atcatacaca gtcatgtgtg ggtaaagggc gtagttttga
    38161 aagaccatgg caatatcacg gtctttagga gacttgtcat tcaccacttc gccaccgatt
    38221 tttaactctc cttcagaaat gtcctcaaga cctgcaatca tacgaagagt agtcgattta
    38281 ccacatcctg aaggtccaac aaagacgata aattctttgt ctttaatgtc taagtcaaaa
    38341 tcttcaacgg cataatgagt agtatttggg tattttttat agatgtgatt tagatttaat
    38401 tcaaccatgt gtaactccta tttgaacagc aaacgttcta gctgactgca aagctagaaa
    38461 ccactgatat cttttatatg atgcttttat tataacaaaa atagcgcttt caaaacagag
    38521 acaaagcgac caaacttctt tgggcaactt gcacaatagc agggctagtc aagtcgtaaa
    38581 gacccataaa acaagaaaac catgtaaaca caggccggtc acaggagcca aaacaagctg
    38641 aacgatctat cattctccct atcagctaca gtccaggcag caaaaaagaa agccctgtgc
    38701 ctttcctaat atttcaacaa aaagagatga gcaaaggcta aatcatccaa ttgcttcaag
    38761 tggagtcctg attgctgagc aaatttatca agtttgtatt gcaaggagtt acggtgaata
    38821 tacaagcgct gcgctgtctg caccagattg ccgtgttctg accatagcgc gtgaaccaca
    38881 tcggccatat cactttgagg acttaggaat tggtgaaact gacgcgtcaa tgccaaaaaa
    38941 gtgtggtgcg acagcaaaga ccacagcatc agtcctgaaa atgttaataa ttttctgcct
    39001 gattgttgcc ttaaataggc cgttaacagc tgacattcct cctcaaaaca ctctctcaat
    39061 gtacccgcag ctacctgata ccaggcattt ccaacaaaca tggttaaggc caaaccaaaa
    39121 tcactttcta gcgttggcag gagatcttca agccagcgca agacctttat tgagattgct
    39181 tgtcggcata aaaatgccgt ttgggttgca ctgataggca aaatgacttc aagccctgca
    39241 atcatttgtt ggagcaactc aactaagtca tctggtaaag cctgctgatg attaaggtaa
    39301 atgaactgag aagtctcata cagctgaggg ggattgccct gatgatcaag caaataccgc
    39361 tgccaaggtg accctagcgg ctgcaagacc tcacactgac ccagccccac ctcaagtaat
    39421 aagcgctctt tttcggataa acatgatttg ggaaaatgca catattggtc cccttctttg
    39481 atactcatcc aatctttatc tcctaacgga tgagggccta cttgcatctc tggaaaatag
    39541 tcttttagct ccatagccat tcctttcatg acggtcttta aaccattata acacatgact
    39601 ctttatcaca cagttcggtt tgttgtcagc acgattttgt attttctgcc tttttaatca
    39661 ttaaagctaa ataatggtta ttcattttta gcaagaacat tcaatcaaat ggctatttat
    39721 cggaagatta atttaattta ttggaagatt aatttatgtt tatactaaaa aagtattgtt
    39781 gccctttttt cattatcatg acattatcat taagatatca tttaaaaaca atcattagat
    39841 ttttgtttgt atctataaaa ttattatgtt attctaataa tggggattga aacttaactt
    39901 ttaggaggtt tctatgaaaa attacttatc tattggagtg attgcactgc tgtttgcatt
    39961 aacatttgga acagtcaagt cggtccaagc tattgctggg tatggatggc taccagaccg
    40021 tccacctatc aataacagcc agttagttgt tagtatggcc ggtatcgttg aaggtaccga
    40081 taaaaaagtt tttataaatt tttttgaaat cgatctaaca tcacaacctg ctcacggagg
    40141 aaagacagag cagggcttaa gtccaaaatc aaaaccattt gctacagata atggcgcaat
    40201 gccacataaa cttgaaaaag ctgacttatt aaaagctatt caaaaacagc tgatcgctaa
    40261 cgttcacagt aacgacggct actttgaggt cattgatttt gcaagcgatg caaccattac
    40321 tgatcgaaac ggcaaggtct actttgctga caaagatggt tcggtaacct tgccgaccca
    40381 acctgtccaa gaatttttgt taaagggaca tgtgcgcgtt agaccatata aagaaaaacc
    40441 agtacaaaat caagcaaaat ctgttgatgt agaatatact gtacagttta ctcctttaaa
    40501 ccctgatgac gatttcagac cagggctcaa agatactaag ctattgaaaa cactagctat
    40561 cggtgacacc atcacatctc aagaattact agctcaagca caaagcattt taaacaaaac
    40621 ccacccaggc tatacgattt atgaacgtga ctcctcaatc gtcactcatg acaatgacat
    40681 tttccgtacg attttaccaa tggatcaaga gtttacttac catgtcaaaa atcgggaaca
    40741 agcttatgag atcaatccta aaacaggtat taaagaaaaa acgaacaaca ctgatctggt
    40801 ctctgagaaa tattacgtcc ttaaacaagg ggaaaagccg tatgatccct ttgatcgcag
    40861 tcacttgaaa ctgttcacca tcaaatacgt tgatgtcaac accaacgaat tgctaaaaag
    40921 cgagcagctc ttaacagcta gcgaacgtaa cttagacttc agagatttat acgatcctcg
    40981 tgataaggct aaactactct acaacaatct cgatgctttt gatatcatgg actatacctt
    41041 aactggaaaa gtagaggata atcacgataa gaataatcgt gtcgttacag tttatatggg
    41101 caagcgccct aaaggggcaa agggtagcta tcatttagct tatgataaag atctctatac
    41161 cgaagaagaa cgaaaagctt acagctacct gcgtgataca gggacaccta tacctgataa
    41221 ccctaaagac aaataaccac gatcttctaa aatgatgaga ttaactgaca aaaaaagcaa
    41281 gcaacacgct atcaacagtt gcttgctttt ttctaacctc ttagttgtag agactagtga
    41341 catttcgtgt ctaaaataat cgtaactggt ccatcattga tgagactaac ctgcatatct
    41401 gccccaaaaa cgccacgctc aactggcaca aactctgcca attgttcatt aaagctatca
    41461 tacaactgac tagccaaatc tggcttagca gcaccagtaa aggcaggacg gtttcccttt
    41521 ttggtgtcag cataaagggt aaattgcgac acagataaga tactgccctt gatgtcttgg
    41581 atagactggt tcatcttgcc atcagcatct gaaaaaatgc gcatgttgac tatttttcgc
    41641 acagcgtaag ccaaatcttc tgcattatca tctggtccca cacctactaa caacaacaac
    41701 ccttgattaa tagcgccagc aattttccca tcaatagaaa cagaggcctc tttgacccgt
    41761 tgcaagacaa gtttcatgtt tttatccttt cttagccatt ggtccgcttc acgctataaa
    41821 catctggaac tgccttgatt ttttcgacaa cagtggtcag atgcgtcaga tttggaatgc
    41881 caaagctcac gtgaatatta gcaaacttca tgtccttggt cggctgagca ttgactgtcg
    41941 atatgctctt ggttgagttt gataaaattt ggagcacatc attaagcaga ccactacgat
    42001 tgagcccata gatatcaatt tcagcctgat aatctttact cgaattgtcc aaatcccact
    42061 cgacctcaat caagcgttct tggtagccat cttgactctt aatgttatga cagtccgatc
    42121 tgtgaatcgc aatgccacgc cctttggtaa tgtagccgtc aataggatca ccaggtacag
    42181 gattacaaca cttggcaatc cgcatcaaga ggcctgatgc tccttggata atgactccat
    42241 tttcactgcg aaccttgagc acatctttgt tttcgtgttt gacctcaccg cccttaacca
    42301 attcttcagc ttctgcttta gccttggccc tttcttcttc acggcgctct ttttcggtta
    42361 acttgttaaa gacactgata ggactaatgt caccaaaccc aacggctgca tagagtgatt
    42421 cttcgctctt cacactgact tttggaagga tggcttcaat gcgttttttg tcaaggtatt
    42481 tattggcaac gtagccctgc tcttgaaaat aagacaccaa caaatcacgg cctttattca
    42541 ctgacaattc cttgtcttga tttttaaaga actgacgaat tttgttgcga gccttattgg
    42601 ttttgaccag ttttacccag tctcgactag ggccaaagga attggcattg gtgatgattt
    42661 caaccacatc tcctgttttt aacttggcag tgagaggaac catacgtcca ttgactttgg
    42721 cacctgttgc tttttcaccg atttgcgtat ggatcgcata agcaaaatca ataggacctg
    42781 attcttttgg taactcctga acggcccctg tcggtgtaaa gacataaatc cgttcagaaa
    42841 aaatgtcttc tttgaccgaa tccacaaagt ccactgcatc gccatttgag gcatcttgca
    42901 attctaccag ctctttgatc cagttcatgc caacggcttg ctcagcttga ttgaccttac
    42961 cacgcacgcc ttttttataa gcccagtgag cagcaacccc gtactcagcc acttgatgca
    43021 tgtccttagt tctgatttga atctcaatag gtccttttgg cccatacacg gtggtatgaa
    43081 tagactggta gccattagct ttaggagctg caatataatc cttgaagcgg cctggcatgg
    43141 gacgccaaag ctcatgaata tagccaacca tagcatagac atcgctttgc gtttccatga
    43201 cacaacgaat ggcaatcaga tcaaaaatct gatcgaatcg tttctttttg tcccgcattt
    43261 tccgataaat cgaataaatg tgttttggtc ggccatacac atctccaaac aacccttgtt
    43321 gtgtcgtata ggttttgacc ttactgacaa tagcctctac caaagcttca cgctcgcgac
    43381 gtttttcttt catcatatgg gaaattttgt aaaattcggt ttcattgagg taacgaaaag
    43441 ccaaatcttc tagttcccat ttgatgcgac taatccccaa acgatgcgcc aagggggcat
    43501 agatttccat ggtttcgcgc gaaatgcgct cttgtttgtc cttgcgcaaa tgtttgaggg
    43561 tgcgcatatt atgcaggcgg tcagccaatt tcaccaaaat cacgcgaata tctttggaca
    43621 tagccatcag cattttgcga tggttttcgg cgagttgctc ctcatgagat ttgtactcaa
    43681 ctttacctaa cttggtgaca ccatcaacga tatcacgagc atcatggcca aagtctgctt
    43741 cgatctcatc taaggtaata tccgtatctt ctacgacatc atgtaaaaag ccacaagcaa
    43801 ctgtcacagc atccagatgc aaatcagcca gaatccccgc cacctgaatc ggatggacga
    43861 tatagggttc gcctgacttt ctcacttggt agaaatgggc cgctgttgca tatgctaaag
    43921 cctttgccac aaaagccaca tcagccttgg tcatataggt ggccgctaag gcaatgactt
    43981 cttctcctgt tacgttcatt atttttgcca tttcgttcct catctagcat ttcttttccc
    44041 tattttaaca ctttacacac aaaagcaaaa ggcagaatct gctaagaaag cgcttagtgt
    44101 gaccttttct tagaaaacgg ttagtgagct gcttttgata tcttttggga tctctcaggc
    44161 gtgagcatga taccaaaggc ctgctcccga ccaaaaatag agagaaccaa ggggctctct
    44221 ctattatgtt tgcttatcag taaggtctct caattcagag aattagttgt tttctttgcg
    44281 ttttgtaacg gctaggacac cagctgttgt catcactgct actgctgctg ctgtaaagaa
    44341 tgggttggct tgctcaccag ttgctggtag acgtctgtgg ttgttggcta caggtgtctt
    44401 agccatattg ccattgcggc gttctggcgt ttggttagct tctttgtcag caggtttttc
    44461 tgcagctgga gttacctctt tgctcttttc tggagctttt tcgcctggtt ggcctgcttc
    44521 gccttctggg ctcttttctg gagctttttc gcctggttgg cctgcttcac cttgtgggcc
    44581 tgctgggcct tgctcacctg gtttgcctgc ctcaccttgt gggcctgctg ggccttgggc
    44641 gcccgtttca cctttttcac cttgtgggcc tactggaccc tgagctccgg tttcaccgcg
    44701 gtcacctttt tcaccctttt cgcctggagc tccatcttta cctggagcgc catctttacc
    44761 agcttcacct tgtgggcctt ggattccagc ttcgcctttt tcaccgcgtt caccggtttc
    44821 acctttgtcg cctttttcac cgcgctcacc tttttcacca gctttacctt ggattccttg
    44881 ctcaccgcgg tcacctttag gaccttgggc gcccctctca ccagttttac cttgtggtcc
    44941 ggctgggcca gcaggaccag tctcaccacg gtctcccttg attccagatt taccaattaa
    45001 atctagaatc tccttaagcc tcttctcagt aaaatctttt agctcctttt gaaaatcttc
    45061 ttctttaaaa taggttccat accactttct ttcccaaaat ttctcattgg acacttctgg
    45121 atatttttta agactttctt cgatctcttt tatttttgac tctctttgac tcgacgtcat
    45181 agtcgtagaa gaaacctcag ccttaacgct gcttgccccg cctccaaagg ctagaaggac
    45241 ggcagcggct gaggttagcc cgtagcgcca gactagtttg ttgagattat ggtgctttga
    45301 tgtcaacata tgttgttctc tctttcttta ttagatggtg tgttttagat aaaaataaca
    45361 tggtagctag acctgattat ttataatagt attataatag agtgtgaaaa cgattgtaaa
    45421 gttctctttt gttgttcatc ttttagattt tgactgatca ctcttttgtg gagatcagag
    45481 ggctactttg gcacttgcct gcctcgtttt taagtcgagg gcatccggat cctttcttta
    45541 aggtcactct tttttgaaat gttacttttt ttgactgctt gtaccttctt gtctggttgg
    45601 ataccgctag gtttcttctt tacttgtttg ttggtcttga ttgtggtaag cttatgaaga
    45661 aaccttgtag gataaaaaaa ggagacactt atgcctcaga ttacccttgt ttttattagc
    45721 ctcagtggca acacccttag ctttgtcaag cggctatccc tttatctagc agacaattac
    45781 gattaccatg tcaaacagat taacattaaa gatttgaagc acgagacttt tcccgtcaag
    45841 gaagagtttg tggcgatttt acccacttat ctagaaggcg gaaatggcgt tgactctggt
    45901 gatgtggaga tcctcaccac tcctttggga gagtttattg ctgcccatgg caacgcccag
    45961 cgttgtcttg ggattattgg ctcaggcaac aaaaacttta accatcagta ttgcctgaca
    46021 gccaaacagt atgccaaacg ctttggcttt ccactgctag gtgattttga gctgcgaggg
    46081 actcctgatg acatttcgcg gttagcccag gtcatcatgg aggccagctc cagacactcg
    46141 tctaacgaca cacagacact gcctaacagc tagacagtcc atagatgatc atagctgttt
    46201 taaaaaagag cgcttttagg acaataacta cagagggccc taccccacgc cttgtgattt
    46261 ctcagccttt gaaaagagcg atcacagtgc ttcttggggc ttgggatcac accaagcact
    46321 cctagaaata cccccatgca gactttagca aggcaactga acgcttggtg gtactttctg
    46381 tgaagctcag atggttggtg acaaaaaagc cctggttttc tggggttact gacatgacta
    46441 aagaggtcta accagctcta ccttgagctg gttagacctc tttgtgttct cctaatcccc
    46501 cctagatgat cgcagtttct tatgtgtctt ggcttctctt atgtggtgtg cctgttagca
    46561 catgctctaa cgccagccta atgtcacttc tagaccatag tggtcactga cgactggcgc
    46621 atctccgcct tcaaaggtaa tcttagaaga tcggatgatg aagtccttac tggtaaagac
    46681 atggtccacc ttgagggctt ctttgttacc ttgccagcca tcaatatcag ccacgatgct
    46741 atgatcacca aacacgtggt cagcaccctt atggctatct tgtaggtcta gggggctacc
    46801 catcatcact tgataaccgt cttgatccgt tggattgtta aaatctccca taagtagcaa
    46861 gggacagttt agtgtgagca gttctttttc taatttctcc cactccccta aaaacccctt
    46921 gtcaaaccaa gataggtgca cattgacaac agcgacttct ttgccatcta acgtggtttt
    46981 ggctatgagt gagcgtctgg tgtggtagtc tgtttcatca tccatcgctg acacgagaat
    47041 atctgaaaca tgaataggtt gttttgataa gattgccacg ccttcctggt aaatgtcata
    47101 gccaatatgg ttataagccc aagaccaata atagtgctgc cctcgttttt gtaaataatg
    47161 gatcaagaga agggcaaaat ggtctttatg aatgcttggt gtccctgaaa gagcttgata
    47221 tcttggtaaa tctgtggcca gctcgctctc gattaactga ttgatttctt gcaagcaaat
    47281 gatatcgtat ttttcagcta aaatatgctc agccaaagca actaattttt taagagtgtt
    47341 ggcttgcatc cacgaatggg tattcagtgt taataccttg gtcattaatc ctcctttata
    47401 agacaagaag agccataggg cctaaaccag ctatggctat tacctatcac tatctcgtga
    47461 tgttttgtta aagttctacg gtagctactt tagtcttagc aggctgagct cctaaagatg
    47521 tcaaggtcac atcttggatc tctgttgtgt tggtaaaagc aacaacaatg attgtttcac
    47581 gctctgctga tttaatagca gcaagatctg ctacgactaa taggtcacct gcatgaaccc
    47641 gttgcccttc tgagactttg actgaaaaag gcacaccatt taaggcgacg gtatcaagtc
    47701 ccacgtgcac cagcacttca agaccattat ctgtcagtaa accaaaggca tgcttggtcg
    47761 gaaagacact ggtcacaagg ccagctacgg gtgcatagat attaccatgg gttggttcaa
    47821 ccgcaaaccc atctcccatc atcttagccg caaacacctg gtcttttaca cctgtgattg
    47881 ggagaaccat cccgtctgcc actgatagca catcttctgt cacgtgttta aaatcttttg
    47941 cgggagtcgg tttgctagtg agctgtgaca tattgacttc tggaataaga gcacctgagt
    48001 ccagcaaatc ttgaatatct gatttcaaaa tatctgcttt agggccatag accgcttgaa
    48061 cgccgttgcc tttttggatc aagcctatag cgccagcttt tttccaatcg tcctcagcac
    48121 caaccttagc ggggtctttg accgtcactc gtaagcgcgt catacaagca tcaacatcat
    48181 caatattgcc tgccccacca aggagattaa cgatttgcac gacttgagag ttgctatctg
    48241 ctacttgggt aggtcttgtt tgagtgttac ggtcacccag tatatcggca tcgtagttgc
    48301 ctaaacggcc agctgttgcg agatgcattt tcttgatcat catatctgca ataaagtaca
    48361 tgataactgc aaagaggaca gaaacccaaa caaagttaat cacatccatg cctaggcctg
    48421 ctttaagagc cataggggtg cgggttagta gctctatatt tccaaatgag tgaacacgga
    48481 gattcacaag gtctgccatg gcaaatgaag ctccttgaac aagggcatag acgatgtaaa
    48541 gtggcattgc tgcaaacata aataggtact caagtggctc agttacccct gtcaaaaaga
    48601 ccgctgccgc tgctgagatg aacatcattt tgtatgtgtg ttttttatcg gcatccacat
    48661 tacggtacat ggctaaggca acccccatca atgttccggt agctccaatc atttgtccta
    48721 ctttgaaacg agctggagtc acactatcca ttaagtgact ataggctgaa gcgtctgatc
    48781 ctttaaggtg aacaaggtca gttacccaag caagccacaa agggtcttga ccaaatactt
    48841 ttgtccccgc tgctgcacct gtcatgacct cataagttcc accaagagct gtatagttca
    48901 ttgggatcgt caacatgtgg tgaagaccaa atggcaacaa gagacgttcc aaggtaccat
    48961 ataggaatgg ggctaggatt ggagctgaat cttgtgaaga ggcaatccac atcccaaaac
    49021 tgttaatccc agactggatc accggccaaa caacaaccaa aataagggct acaaaaatag
    49081 aacgtaaaat aacgacaaat ggtacaaaac gcttcccatt aaagaaggtc aaaacttcag
    49141 gaagtttacg gtaattatag tatttgttat aggccgttgc tcctacgaac cctgcaataa
    49201 tcccaacaaa aacccctgtg tttaaagctg gagattccaa gacactggtg aaataatctt
    49261 taacgatcat ttgagtgcca aggaggcttg tgatttttgc ttctggatca gctagcatag
    49321 tactagacac gccataaaaa gcacctgtta tccgattgat caagacaaaa gcaagccctg
    49381 aggcaaaggc ccctccagct ctttctttag cccaactacc accaatggcc aaggcaaata
    49441 gcaagtgaag gttaacaata acagcccaac caatttgagc gataatgttg cctagagatg
    49501 ccaaaaatgc tgagtcgtgg ttaatcattg gaatagagtt tccgatactg atcattaaac
    49561 cagccgctgg catgacagca ataacgacca taagacattt cccaaatttt tgccaaaatt
    49621 cgaaacgaaa cagttgcttg aatgatgttt tcattctatc tctcctttat tatccccttt
    49681 tagccatagc tgcccttaag gcgcttttgt gacacaatga tatagacagc aactcagtga
    49741 tgcaatcgct tgcattgatc tgataccttt attatactag cctcgcaaca atttgtaaag
    49801 cgttttctta gacagatgtt agtcgttttt tttcattaat atagtagtat aactcaccaa
    49861 aaagaggaca agagcccatt tccttttttg gtgatgagcc catcaacgct cttcgaaaaa
    49921 acaagaagca ataaaaaaag gccgttgaca caatcgtgaa ctgcctgtcc gtttctttag
    49981 cctcttcttt ctaaagtaag caacagctta cttattttga tgcctgcaat tccaaagcat
    50041 aactaagagc acttaaggca tatagcggcg ctgtctctgc tcgcataatg cgagggccta
    50101 agcccacctt aatggcactt gctgcctcaa actgagtgat ttcagttggg gaaattcccc
    50161 cttctggtcc aaagataaag aggatttttg cccctggctt aacttctttt acttcacgcg
    50221 ccaaggtcgc tagctgacct gccttagctg tttcctcata agcaatgaag atatgatcaa
    50281 agctagaaag gctttttaga aactctgctt tatgttcaaa aaggtgaact tcaggcaccc
    50341 tatttcgttt gctctgctca gccgctccta atacaatctt agccaatttg tcttcttttt
    50401 tagctaattt tttgccatcc catttgacca ctgaccagtc tgctgggtag ccccatagcg
    50461 cactggctcc caattcagtc actttctggg caatcgtgtc taacttatcg cctttaggaa
    50521 atccagaagc aatagtcact ttaacaggca attccacctg atctggcaaa gcctctataa
    50581 tctctaactc atgagccata ctattggtca ccttagctaa atacttcacc ccatcatcaa
    50641 agaccaagac aacttcagcc tcgtcagcca agcgcatcac ttgaaacatg tgcttgatgg
    50701 tatctttatc ggtaatcgtt accttttttt ctgctttgcc tttaataaaa tactgttgca
    50761 ttagccgcca atcactcctg agatatcatc tgttttttta aagacaaggg cattccactc
    50821 accttgaacc atgtgagttt ctaagaaaaa gcctgcgcta aaggcagctt ccagaaccat
    50881 atccaatttc tcagaaatga tgccagataa gataaggtaa ccttcctttt tgaccaaacg
    50941 gtaagcatca tcagtcaata agactaagat atccgccaag atattggcta caatcacatc
    51001 cgcttcttgg ctaacccctt ttaaaaggtc accagcagcc acatgaatgt tgtctgttcc
    51061 ttggtttaaa tcaatgttgt cttgagccac tcgaacagcc acgtcatcta agtcataagc
    51121 gtagatggtt ttagcaccta gtaaggaact ggcaatcgag aggacacctg aaccggtccc
    51181 tacatcgata actgtctcac caccacgaag gatttgttcc aaggcaaaaa ggctcatctt
    51241 agttgtggga tgtgtccctg tcccaaatgc cattccagga tctagtttaa tcactttttc
    51301 cccagcagaa gcgtcataat ccgtccaact agggacaatg gttaaatcat gcgtgatgcg
    51361 agctggttca tagtattttt tccaattgtc tgcccagtct tcctcagcca attcttgact
    51421 gtccacagta acctgaccaa cctgtaaccc aaaactagct aactccgcta actgttcatt
    51481 aatggtcgca ataatatccg ccaaattagt actacttggg taataggctg tgatagctat
    51541 catgtcagac tgctccacat ctgggtataa ctccccaaag cggtcctttt gcccaatata
    51601 gtctgcgcta tctgcgatag caaccccttg actgcctgtc tcaatcaaca catgtgaaac
    51661 agcctcttgc gcatcacgat gcacatgaac cgtcacttct tgccacgttt ccatagttat
    51721 gtcctttcta ttttgacctt ttggttattg tcctaatagt gttagcacca tctcatgtgt
    51781 caccttagta ccttgggtaa gggtaacttg ctgcttcttc tagtccttgt tgaccttctt
    51841 caaaagcttg cgcgttccac tccaagaaga aatcagcctt gctgtcatct gctaagaaat
    51901 ccatcaaatc gctgtgccct tcaatagcaa catcgttgag aaattgcgca aagtaagcta
    51961 agaattcacg agaaaagccc tttttagcgt caaaaggaat agtaaccaaa taatctgctg
    52021 catcaaattg agacttggca gggttataaa agagcacata gtcctcaaag acaatatcat
    52081 cttgggtcac ttcgccacta tcgtcaatgg tttttatagc ctctttattt tgagcttcta
    52141 acacaaaagt cacttcaacc gcatgatttt tcttgtccca atccatagca tagtcatact
    52201 gaaaatgctt atccatttct tcttctaata ctgataaaaa gccgtatgtt gccatctttt
    52261 ttatacctaa tctttctaac tgttgctgta taaggctttg taagacctca ttagtttact
    52321 tactttgaca tgctgagcgt actgttataa cttttattaa ctgaattata acatatttga
    52381 ctaatattga ccataagaga aggtcttaat ggcaggatca cctctagcaa ccgcttttat
    52441 gatataattt gggtatgcat agaaaaacta ttattgattt caaagagctc gggcaacgct
    52501 atctttttga tgagccatta gtagaattgg tagccaagtc cttagatcag gtcggtccag
    52561 tcattgaaaa agtacaacac taccaacagc tgggctatta cgtggtgggc tatctcagtt
    52621 atgaagcagc tgcttttttt gacaatgccc tacaaaccca taatgacaga ctaggcaacg
    52681 agtaccttgc ctattttaca gtgcacaaaa cctgtcaaaa aaaagaccta cctcttgatt
    52741 atgacagcat tactatccct aaccaatggg tcagcgcaac tcaaaaagaa gcctatcaaa
    52801 aagccattga aaccattcac cgtgagatgc aacagggcaa tacttatcag gttaactaca
    52861 cccttcaatt aactcaggaa ctcaatgcag ctgatagttt agccatttac aataaactcg
    52921 tcgttgaaca ggctgctggc tacaatgcct acatcgccca tgatgagttt gccgttattt
    52981 cagctagtcc tgaattgttc tttaaacaag agggcaacag gttaactact aggcctatga
    53041 aaggcaccac caaacgaggg gttaatagct ggctggatca acaagaacat gactggctcc
    53101 aagctgacgg gaaaaaccgt tctgaaaaca tgatgattgt ggatttgctc cgcaatgaca
    53161 tggggaaaat ttgccaaaca ggcagtgttc gtgttgatag gctctgtgag gtcgagcgat
    53221 actctaccgt ctggcaaatg acctcaacta ttgttggtga cctcaaagcg gactgtgacc
    53281 tgatagacat tctcaaagct ctctttcctt gtggctctat cacaggggca ccaaaagtct
    53341 ccaccatggc aattatcaca tctctggagc caaaaccaag ggggatttat tgtggaagta
    53401 ttggcatttg cctacctgat ggtcgccgtt tcttcaatgt tcccattaga accatccagc
    53461 tcagtcataa tcaagctact tatggtgtcg gaggcggcat cacttggcaa agcaagtggg
    53521 aagacgaata cgaggaagtc catcaaaaaa cagcttttct ctatcggcac aaacaaatct
    53581 ttgacctcaa aacaaccgcc aaggtggaac acaaaaaaat agccttcctt gagcaacacc
    53641 tcaatcgcct taaagaagca gccacttatt ttgcttaccc ttataatgaa aaagctttgc
    53701 agaaacaact gtcaacctat ttggaaaata agaataatgc tgcctaccgt ttgatgattc
    53761 gtttatccaa agatggaaag attagcctct ctgatcaacc cttggaacct ttgtcagccg
    53821 attttctgac agctcaactc tctttacaga aaaaggacgt gacagcttcg ccttttacct
    53881 actttaagac cagctaccgg cctcatattg agcaaaaatc ttatgaacag ctattctata
    53941 accaagcggg gcaattattg gaaacgtcca ttggcaatct ctttgttcag ctgggtcaga
    54001 ccctctacac accaccagtg gctgtgggga ttttaccagg actttttcgc caagaactgc
    54061 tagctactgg tcaagctcag gaaaaagagg tgacactagc tgatttaaaa gaggctagcg
    54121 ctatttttgg aggaaatgct gtccgtggcc tttatcctct caaccttgag cttactcacc
    54181 ttgatgccct cttagctaag agtcaagcct aaactgccac tcatcttttg ttattaagga
    54241 acctatgata ctcttaattg ataattacga ttcatttacc tacaacctcg cccaatattt
    54301 aagtgaattt gacgagacga ttgtcttgta taaccaagac ccaaacttat atgacatggc
    54361 caaaaaagct aacgctctag tcctctcacc tggtcctggt tggcccaagg aagccaacca
    54421 aatgccaaaa ctcattcaag acttttacca aacaaaacct atcttaggag tgtgtctggg
    54481 acaccaagct atcgctgaaa ctttaggggg aaccttacgc ttggccaaac gcgtcatgca
    54541 tgggagacaa agcaccattg aaacgcaagg ccctgctagt ctttttcgct ccctgccaca
    54601 agagatcacc gtcatgcgct accattccat cgttgtggat cagttaccaa aaggttttag
    54661 cgtaaccgct agagactgtg acgatcaaga aatcatggca tttgaacacc acaccctgcc
    54721 actttttggg ctacaatttc acccagaaag catcggaact cctgatggca tgaccatgat
    54781 tgccaacttc atcgcagcca ttccccgtta atctcatccc ccaaaagaaa ggaggacagt
    54841 tacatgcctg atcatttagc gctcagaatg cgccccaaaa ccatttcaga ggtcatcgga
    54901 caaaaacacc ttgttggcga aggcaaaatc atccgtcgca tggtggaagc aaatagacta
    54961 tcgtccatga tcctctatgg ccctccagga atcggtaaaa cttccattgc ttctgctatt
    55021 gctggtacta ctagatatgc ctttcggacc tttaatgcca ccattgacag caaaaaacgt
    55081 ctccaagaaa ttgcggaaga agctaaattt tctggtggct tggtattatt actagacgag
    55141 attcatcgcc ttgacaagac aaaacaagat tttcttttac ctttacttga gaatggcacc
    55201 attatcatga ttggcgcaac cactgagaac cccttctttt cagtgacacc cgctatcaga
    55261 agcagggtcc aaatttttga attggaacca ctttctaacg aggatattaa aacagccatt
    55321 cagctagcaa tttccgacaa agaacgggga tttccttttt tagtgacaat tgatgatgag
    55381 gcgctagatt tcattgttac cgccacaaat ggagacctac gatctgctta taattccttg
    55441 gatctagcag tcatgtctac ttctcctaat gaagatggta gtcgtcatat tagtctagag
    55501 acaatggaaa atagtctgca gcgtagttat attaccatgg ataaaaacgg ggacgggcat
    55561 tatgatgttt tatctgcctt acaaaaatcc attagaggtt ctgatgtcaa tgctagcctg
    55621 cactatgccg ctcgtttagt agaagctgga gacttgccta gtttagcaag acgattgacc
    55681 attattgctt acgaagatat tggcttagcc aaccctgacg cccaagttca taccgtcacc
    55741 gcactagatg ccgcccaacg cattggtttt ccagaagctc gtattcttat cgctaacgtg
    55801 gtcattgatt tggctctatc acctaaatcc aattcagctt atttagctat ggatgcagct
    55861 ttagctgatt tgagaacatc aggcaacctc cccattccaa gacatcttag agacgggcat
    55921 tatgctggca gcaaggacct aggcaatgcc aaagattacc tctacccaca cgcctaccct
    55981 gaaaaatggg tcaaacaaca atacctacca gataaattag taggacatca ttattttgaa
    56041 gccaatgaaa ccggtaaata cgaacgcgca cttggttcaa ataaagagcg tattgataag
    56101 ctctcagact gactgctagt tttaaaaatc gcaaccgatt tcaaaaatcg ataattttgc
    56161 ttgaaaaaat tttaaaaagt ggtattataa aagtacagaa acattgctgt ggcatacgaa
    56221 tccacatcta agtgttgacc gactatttct ttgtattttc gggaaacaaa gactctttct
    56281 agtctgcaag ccgtttcacg cggacgcaac ttccaagaaa gagcgcgtta cccacctgct
    56341 tatactgcgc gggctcaata caaaaacgtg gatcaccggt gccaatacag cattcttgcc
    56401 tccttagctc agttggtaga gcagtagact cttaatctat gggtcacagg ttcgagccct
    56461 gtagggggta taaactatac atgaaaaaac ctttgtttaa aggctttttt tgcttgtcag
    56521 aagaggattt gttccatcat ttatccccgc agtaattttt tatggtatga taccaattaa
    56581 agcgcttaag attaaggtgg agactgttat gaaaataatt gagtacaaag gaaattacag
    56641 agatgacctc atttttatgg tcttggaagc aaaaaatgcc ttaaggagaa ttcccagcat
    56701 taacccggat cttttgacaa ttgaaacaag ttatttggaa aaagaggatt gtttttggct
    56761 agcggtcaat gaagaaagtc gtgttattgg ctcaatcgct tattcttcta ttcccaaaac
    56821 aaatgaggtc ttcttgcacc gtctcttcat caaaccaagc ttaaaacatc aaggcattgg
    56881 cagtcaactt ctggcgtttg cagaagatta tctaaggaaa atcggcaaga gtcatgttcg
    56941 tatccactta ggtcaaccta aagaacaatg gtttgaatct tatcggtttt atcctaaaca
    57001 tggttatcaa ttctatgatg acacgcatct catcaagaaa ctaatctaaa cacatttcct
    57061 accctttata gtcatttcat acgtcatttt taggaggtat ccattgaaaa ttgccatcat
    57121 tggacatagt ggctctggca agtcaacctt agcacgattt ttaggccaac attaccattg
    57181 tgaggtattc catttagacc aactacactt ctcatcgaac tggcaagaac ggtcagatca
    57241 tgacatgata gctgatttat caacatgcct gctcaaacaa gatttgatta ttgaggggaa
    57301 ttatgccaat tgtctctatg aagagcgcat gagtgaagct gattacatta tctatgttaa
    57361 tttcagtcgg tttcattgtg tctatcgagc atttaagcgt tatttaaact ataggggaaa
    57421 aacgcgtcct gatatggctg acaactgtca ggaaaaattt gacgttgcgt ttgtgaaatg
    57481 gattctacta gatggacgtt caagaaacca attaaaaaaa taccaatccg ttgtccaaaa
    57541 atactctcat aaaaccattg tcttaacaaa tcaaaagcag ctaagtcatt atatgaatac
    57601 gatcaactaa ttagagtgca aaactaaaat aaccacgggt aatccgggct attcagactg
    57661 aaagcctttg tttaaaggct tttttgcttg tttcataact aatcggctta attaaaagcg
    57721 tcaatccact tttagtatca tgtctcaatt ttcattcgat agtcagcaag tatatctaca
    57781 aaattgttaa tgattatttc ttttgaaagt gttatattaa aacataataa aataataact
    57841 cctgaaaaga ggctatttat gaaaaaaaca aaacttattt tttcttttac ttcaatattc
    57901 attgcaataa tttctcgtcc tgtgtttgga ttagaagtag ataataattc ccttctaagg
    57961 aatatctata gtacgattgt atatgaatat tcagatacag taattgattt taaaaccagt
    58021 cataacttag tgactaagaa acttgatgtt agagatgcta gagatttttt tattaactcc
    58081 gaaatggatg aatatgcagc caatgatttt aaagatggag ataaaatagc tatgttctcc
    58141 gtcccatttg attggaacta cttgtcagaa ggaaaagtca tagcatatac ctatggtgga
    58201 atgacgcctt atcaagaaga accaatgtct aaaaatatcc ctgttaattt atggattaat
    58261 agaaagcaga ttcctgtccc ctataatcaa atatcaacta acaaaacaac agttacagct
    58321 caagaaattg atctaaaggt tagaaaattt ttaatatcac aacatcaatt atattcttct
    58381 ggttctagct acaaaagtgg taaattagtt tttcatacaa atgataattc agataaatat
    58441 tctctcgatc ttttctatgt aggatataga gataaagaaa gtatttttaa agtatacaaa
    58501 gacaataaat ctttcaata
//
LOCUS       DAXWIQ010000007        54375 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000007 DAXWIQ010000000
VERSION     DAXWIQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 54375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54375
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.007"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            1..1497
                     /locus_tag="ACGEUI_000395"
     CDS             1..1497
                     /locus_tag="ACGEUI_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HFF8165257.1"
                     /translation="MQQSMTKVKYLWKENNNYIYSTFLTLLLTVILYANSLNLPLKFI
                     SLLFVVSTLLIVFLPKKLEVATIIFILVFGTISAIISPINDIPDEYVHYSRTVYISEG
                     DINLTNNNKKLRISKDVDKLIKQSGKTFITSNLKATKHSTREYSYPYIKGTNAYYSFS
                     YIPQALGILVGNALDLPILLTYYFGRLCNLISYAMLAFIAIKLSGSFKQVIAVVTLLP
                     MNIYLAASFNQDGFAIGLVLVTIGLFINLLSSKDKSNYNTKFFLYLVLCGLLVLSKFT
                     YFLLVCLPLFIPNEKFGKNTKLVILKKLGGLLLIFLFAAMWFRLYGQVKTPYVADFLK
                     EVNVSQQVKNMLESPIVYSSIIIRHMVINLINMNNIFQFGALSYGITNLFPLYVCFFF
                     FVYISNASKITINIVEKMGIIFVISAIIGATVLAMYLTWTPVGSSTVLGVQSRYLIGI
                     IPLVLLLFSSQQQKFKQIEDILSDKLAIHVSLLFILAMLMSTIFRYYH"
     gene            1591..2814
                     /gene="pepT"
                     /locus_tag="ACGEUI_000396"
     CDS             1591..2814
                     /gene="pepT"
                     /locus_tag="ACGEUI_000396"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFF8165258.1"
                     /translation="MKYDNLLDRFIKYVKVNTRSVPDSETTPSTESQEAFALTILKPE
                     MEAIGLQDVHYNPVNGYLIGTLPANNPTLTRKIGFIAHMDTADFNAENVNPQIIDNYQ
                     GGDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
                     QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALE
                     VTFLGRNVHPGTAKDQMINALELAIDFHEKLPAKDRPEYTDGYQGFYHLTGLTGTVEE
                     ARASYIIRDFEEASFEARKVKVENIAQSMNAQLGTKRVLVELNDQYYNMKKVIEKDMT
                     AIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQT
                     MERAVDVIIGLVCKA"
     gene            2855..3343
                     /locus_tag="ACGEUI_000397"
     CDS             2855..3343
                     /locus_tag="ACGEUI_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFF8165259.1"
                     /translation="MIKLFGKIRYHWQPELSWSIIYWSIAFAPIFVGLSLLYERTEIP
                     SRVFILFAIFAVLVGIGLHRYFIIENNGILRIVSFKLFGPRKLLISTITKIEVTKSTL
                     CLHVEDKSYLFYMRKWPKKYFLDALAVNPYFQGEVILSDNFIKLDYFEVYQHDKKALT
                     RG"
     gene            complement(3330..3527)
                     /locus_tag="ACGEUI_000398"
     CDS             complement(3330..3527)
                     /locus_tag="ACGEUI_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFF8165260.1"
                     /translation="MKVSIIPEKCIACGLCQTYSSLFDYHDNGIVTFSSSSETSQSIC
                     PSDKDAILAVKSCPTKALTLE"
     gene            3576..4052
                     /locus_tag="ACGEUI_000399"
     CDS             3576..4052
                     /locus_tag="ACGEUI_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFF8165261.1"
                     /translation="MAKEPWEEKIVDDTIGTRTRKSRNAFISTPWLTALLSVFFVIIV
                     AILFIFFYTSNSGSNRQAETNGFYGASTHKKTRKASNAKKTSSSSTTTDTTPSSEETL
                     ASSEGTGETLTVLAGEGAASIAARAGISVEQLQALNPEHMTQGYWYANPGDQVTIK"
     gene            4067..4747
                     /gene="cmk"
                     /locus_tag="ACGEUI_000400"
     CDS             4067..4747
                     /gene="cmk"
                     /locus_tag="ACGEUI_000400"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFF8165262.1"
                     /translation="MKAIKIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYI
                     ALTHGYTGKEVALILEELEKNPIFFKKAKDGSQLVFLGDEDVTLAIRQNDVTNNVSWI
                     SALPEIREELVHQQRRIAQAGGIIMDGRDIGTVVLPDAELKIFLVASVEERAERRYKE
                     NLEKGIESDFETLKEEIAARDYKDSHRKVSPLKAAEDALIFDTTGVSIDGVVQFIQEK
                     AEKIVDMS"
     misc_feature    4771..4894
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            4909..5439
                     /gene="infC"
                     /locus_tag="ACGEUI_000401"
     CDS             4909..5439
                     /gene="infC"
                     /locus_tag="ACGEUI_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFF8165263.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQSLADA
                     SNVDLVLIQPQAVPPVAKLMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLADFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPISDKK"
     gene            5481..5678
                     /gene="rpmI"
                     /locus_tag="ACGEUI_000402"
     CDS             5481..5678
                     /gene="rpmI"
                     /locus_tag="ACGEUI_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFF8165264.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAFTSHRFHGKTKKQRRHL
                     RKAGLVSSGDFKRIKAMVTGL"
     gene            5737..6096
                     /gene="rplT"
                     /locus_tag="ACGEUI_000403"
     CDS             5737..6096
                     /gene="rplT"
                     /locus_tag="ACGEUI_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFF8165265.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVAD
                     AAAFTALADAAKAKLGK"
     gene            complement(6387..8558)
                     /locus_tag="ACGEUI_000404"
     CDS             complement(6387..8558)
                     /locus_tag="ACGEUI_000404"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFF8165266.1"
                     /translation="MKKFKTLITGFINTRLGFIITLLFCYWIKTLWAYHTDFSLDLGN
                     IYQVFLTIINPIPLAFLLLGVALYVKNTRAFYICSWVVYIILNILLISNSIYYREFSD
                     FITVSAMLASSKVSAGLGDSALNLLRIWDIIYILDFIILISLSIAKKIKNDQRPFNKR
                     AAFAITALSSLLLSINLFLAEIDRPELLTRGFSNTYIVRALGLPAFTLYSGNQTYQAQ
                     KERNGATAEELIDVKTYVKGHYAAPDPQYFGIGKGKNIIVLHLESFQQFLIDYKLKEG
                     DKEYEVTPFINSLYHSNATLAFPNFFHQVKAGKTSDAETMMENSLFGLNSGSFMVNYG
                     GENTQFATPSILAQKGGYTSAVFHGNVGTFWNRNNAYKQWGYNYFFDSSYFSKQNSKN
                     SFQYGLNDKYMFKDSIKYLEQMQQPFYTKFITVSNHYPYTSLKGESSEEGFPLAKTDD
                     ETINGYFATANYLDAALKSFFDYLKATGLYDNSIFVLYGDHYGISNSRNSSLAPLLGK
                     DSETWSEYDNAMLQRVPYMIHIPGYTNGSIKETFGGEIDALPTLLHILGIDTSQFVQL
                     GQDLLSPQNSQIVAQRTSGTYMTPEYTNYSGRLYNTQTGLEITNPDEMTIAKTKEIRS
                     AVAQQLAASDAIQTGDLLRFDTQNGLKAIDPNQFIYTKQLKQLKDISAKLGSESTSLY
                     SKNGHKSTQKLFKAPSYLELNPVEADAATSELKEDNPKNKE"
     gene            8705..9868
                     /locus_tag="ACGEUI_000405"
     CDS             8705..9868
                     /locus_tag="ACGEUI_000405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8165267.1"
                     /translation="MNKLYIDSFVEKKLTAGVQLLDEKDFSNIKEKNQLVQLVTKSNR
                     PIGTAYISKQNKGIGWYLGPEKIDLSISYFVSLFSVAKAKRQDFAQSDETNAYRLFNQ
                     EGDGFGGVTIDLYKDFAVFSWYNAFVYDKKEMIMEAFQQVFPEVKGAYEKCRFKGPDT
                     ETAHLYGELAQETFSILENGIAYQVFLNEGLMTGIFLDQHDVRRALVDGLAMGKSLLN
                     LFSYTAAFSVAAAMGGAIETTSVDLAKRSRELSLAHFEHNQLNLASHHFVVMDVFEYF
                     KYAKRKKLIFDVIVIDPPSFARNKKQTFSVSRDYHKLITEALDILSPKGTIIASTNAA
                     NMTVSQFKKQIIKGFGSRRPESMTLQQLPSDFTINKADERSNYLKVFTIKVRE"
     gene            9865..10551
                     /gene="aroD"
                     /locus_tag="ACGEUI_000406"
     CDS             9865..10551
                     /gene="aroD"
                     /locus_tag="ACGEUI_000406"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFF8165268.1"
                     /translation="MRIVAPVMPRHFDEAQAIDISKYEDVNLIEWRADFLPKDEIVAV
                     APAIFEKFAGKEIIFTLRTVQEGGNITLSSQEYVDIIKEINAIYNPDYIDFEYFTHKS
                     VFQEMLDFPNLILSYHNFEETPENLMEAFSEMTKLAPRVVKIAVMPQSEQDVLDLMNY
                     TRGFKTLNPEQEFATISMGKLGRLSRFAGDVIGSSWTYVSLDHVSGPGQVTLNDMKRI
                     IEVLEMDISN"
     gene            10645..11811
                     /gene="aroC"
                     /locus_tag="ACGEUI_000407"
     CDS             10645..11811
                     /gene="aroC"
                     /locus_tag="ACGEUI_000407"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFF8165269.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLTLHPADIDHELQRRQGGYG
                     RGARMSIETDRVQISSGVRHGKTTGAPITLTVINKDHQKWLDVMAVGDIEETLKLKRR
                     VKHPRPGHADLVGGIKYHFNDLRDALERSSARETTMRVAVGAVAKRILAELGIDMLHH
                     ILIFGGITITIPSKLSFRELQERALHSELSIVNPKQEEEIKTYIDKIKKEGDTIGGII
                     ETIVQGVPAGLGSYVQWDKKLDAKLAQAVLSINAFKGVEFGAGFDMGFQKGSQVMDEI
                     TWTPTQGYGRQTNHLGGFEGGMTTGQPLVVKGVMKPIPTLYKPLMSVDIDSHEPYKAT
                     VERSDPTALPAAGVIMENVVATVLAKEILETFSSTTMSELQKAFSDYRAYVKQF"
     gene            11872..12213
                     /locus_tag="ACGEUI_000408"
     CDS             11872..12213
                     /locus_tag="ACGEUI_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HFF8165270.1"
                     /translation="MSQEIYDYANQLERAVRALPEYQKVLEVKEAIQADVSASELFDE
                     FVAMQEKIQGMMQSGQMPTAEEQTSIQELSQKIEANDQLKAYFEAQQALSVYMSDIER
                     IVFAPLKDLVK"
     gene            12434..13786
                     /gene="gorA"
                     /locus_tag="ACGEUI_000409"
     CDS             12434..13786
                     /gene="gorA"
                     /locus_tag="ACGEUI_000409"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFF8165271.1"
                     /translation="MVIPYDYIVIGGGSAGIASANRAAMHGAKVLLAEGKEIGGTCVN
                     LGCVPKKVMWYGAQVADILGTYAKDYGFDFKEKAFDFKQLKANRQAYIDRIHASYERG
                     FEQNGVDRIYDYAVFKDAHTVEIAGQLYTAPHILIATGGHPVFPDIEGAQYGISSDGF
                     FALDEVPKRTAVVGAGYIAVELAGVLHALGSKTDLFIRHDRPLRSFDKTIVDVLVDEM
                     AVNGPRLHTHAEVAKVVKNTDESLTLYLKDGQEVEVDQLIWAIGRKPNLEGFSLDKTG
                     VTLNDKGYIETDAYENTSVKGIYAVGDVNGKLALTPVAVAAGRRLSERLFNGKTDEKL
                     DYQNVATVIFSHPVIGSVGLSEEAAVKQYGQEAVKTYQSRFTSMFTAITNHRQPCLMK
                     LVTVGDTEKIVGLHGIGYGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSEEFVTMR
                     "
     gene            complement(13874..15142)
                     /locus_tag="ACGEUI_000410"
     CDS             complement(13874..15142)
                     /locus_tag="ACGEUI_000410"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFF8165272.1"
                     /translation="MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHV
                     VGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFHDRICLNGQPISDLELAQCVSVI
                     RPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHAL
                     AVVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGS
                     RLYQLGKDFLLNPSEKTFSFQHDNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFP
                     KISPKTIQKGIEATTWPGRSEFIQPNLLLDGAHNPDSIAKLKSLLQEEFPKRPIHILF
                     AGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDE
                     LFVVTGSLYFISEVRQYCLSNANIFKNIFP"
     gene            complement(15172..15612)
                     /locus_tag="ACGEUI_000411"
     CDS             complement(15172..15612)
                     /locus_tag="ACGEUI_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6556 family protein"
                     /protein_id="HFF8165273.1"
                     /translation="MSSRYSRQQKPRKSGGVPTEHIKTGFTAFQKSIALIGSILSIIV
                     ATITISRALQPVPDKTSNDTPKESSNTIVKIIEKESSQGNQNHTGTSETTDDNKTTLP
                     SSSTTPSHNETTPTAPETPSATEGTQIPNATGAEDANTGLPTNP"
     gene            15847..16989
                     /locus_tag="ACGEUI_000412"
     CDS             15847..16989
                     /locus_tag="ACGEUI_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFF8165274.1"
                     /translation="MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRI
                     LEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGIAFEKNAFGKHIIISAIEHPAVS
                     ESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAI
                     SNLLANQPTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGK
                     RLNPLLSGGGQEQELRSTTENVAGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKSLS
                     AYPDVTVFSAQEGFAPNILTFGIRGVRGEVIVHAFEKYEIYISTTSACSSKAGKPAGS
                     LVAMGIPVKTAQTAVRISLDDDNDMGQVEQFLTIFKQIYDKTQKVR"
     gene            17001..18215
                     /gene="thiI"
                     /locus_tag="ACGEUI_000413"
     CDS             17001..18215
                     /gene="thiI"
                     /locus_tag="ACGEUI_000413"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFF8165275.1"
                     /translation="MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAITVR
                     SDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLYRD
                     GLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
                     ISYEEIKGTGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPG
                     ALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIR
                     EQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTEITPEIV
                     NDEVENLIDALL"
     gene            18352..19545
                     /locus_tag="ACGEUI_000414"
     CDS             18352..19545
                     /locus_tag="ACGEUI_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFF8165276.1"
                     /translation="MIKTMQYKKTMATVVALIVALLLFGLIYDLLGVQKNELAAQKSA
                     QPKVKTARVVANGDILIHDILYMSARKADDTYDFTPYFEYVKDWISGADLAIGDYEGT
                     ISPDYPLAGYPLFNAPEEIAGALKNTGYDVVDLAHNHILDSQLDGALNTKKVFHQLGI
                     DSIGIYDKDRSKESFLIKNVNGIKIAILGYSYGYNGMEATLSQEDYEKHMSDLDEAKI
                     KKELQLAEKKADVTIVMPQMGTEYALEPTAEQKELYHKMIDWGADVVLGGHPHVIEPS
                     ETVIKGRQKKFIIYSMGNFISNQRLETVDDIWTERGLLMDLTFEKKDNKTKIKTVEAH
                     PTMVLAKGKGIVGKEGFELYNYRTMVLEDFIKGGKYHDKIDEETKAKVALAYQEINDL
                     VNLKW"
     misc_feature    19650..19724
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            19759..20073
                     /gene="rplU"
                     /locus_tag="ACGEUI_000415"
     CDS             19759..20073
                     /gene="rplU"
                     /locus_tag="ACGEUI_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFF8165277.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKIDAEAGAEVTFNEVVLVG
                     GDKTVVGTPVVEGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            20085..20411
                     /locus_tag="ACGEUI_000416"
     CDS             20085..20411
                     /locus_tag="ACGEUI_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFF8165278.1"
                     /translation="MIKAIFTRQKNGQLSSVTLTGHAGSGKHGFDIVCASVSTLAINF
                     VNSLEVLADCQALVDLNDVEGGYMAITIPPHDNKEEVQLLFESFLLGMTSLAKDSSKF
                     VNTQVI"
     gene            20439..20732
                     /gene="rpmA"
                     /locus_tag="ACGEUI_000417"
     CDS             20439..20732
                     /gene="rpmA"
                     /locus_tag="ACGEUI_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFF8165279.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPVAK"
     gene            21080..21994
                     /locus_tag="ACGEUI_000418"
     CDS             21080..21994
                     /locus_tag="ACGEUI_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8165280.1"
                     /translation="MNIQQLRYVVAIANNGTFREAASKLFVSQPSLSVSIKDLEAELG
                     FQIFNRTTSGTVLTSQGLVFYEKALEVVKSFDSFEKTFSQADLDQNEFSIASQHYDFL
                     PPLITAFSQQYDGHRVFRIFESTTIQILDEVAQGNSEIGIIYLNVDNQKGLFQRMDKL
                     GLEYVSLIPFTTHIYLSKTHPLANREALYLNDIQGLPAVRFTQERDEYLYYSENFVDT
                     SECPRIYNVSDRATLNGILERTNAFATGSGFLDHRSVNGIKVIPLADHIDNQMIYVKR
                     KDKNLSVAGATFVTILKDYFDKRRKALT"
     gene            21991..22449
                     /gene="lspA"
                     /locus_tag="ACGEUI_000419"
     CDS             21991..22449
                     /gene="lspA"
                     /locus_tag="ACGEUI_000419"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFF8165281.1"
                     /translation="MKKRLFVLSLILLVALDQLSKFWIVSHIALGEVKPFIPGIVSLT
                     YLQNNGAAFSILQDQQWFFVVITVLVIGYAIYYLATHPHLNIWKQLALLLIISGGIGN
                     FIDRLRLAYVIDMIHLDFVDFAIFNVADSYLTVGVILLLICLWKEEDYGN"
     gene            22439..23329
                     /locus_tag="ACGEUI_000420"
     CDS             22439..23329
                     /locus_tag="ACGEUI_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8165282.1"
                     /translation="MEINVITSGQRLDKALADLSPLSRGQANDQIKQGLVLVNGQQKK
                     AKYTVQAGDVICFELPKEEVLEYQAQNIPLDIIYEDDALAIINKPQGMVVHPSAGHPS
                     GTMVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKTDAAHQALAEELKAKKS
                     LRKYLAIVHGNLPNDRGMIEAPIGRSEKDRKKQAVTAKGKEAVTRFTVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDPLYGPRKTLSGHGQFLHAKTLGLTHPMTGKEM
                     IFTVEAPEIFQKVLKLLRKK"
     gene            23725..24246
                     /gene="pyrR"
                     /locus_tag="ACGEUI_000421"
     CDS             23725..24246
                     /gene="pyrR"
                     /locus_tag="ACGEUI_000421"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFF8165283.1"
                     /translation="MKTKEIVDDVTMKRAITRITYEIIERNKQLDNVVLAGIKTRGVF
                     LARRIQERLHQLEGLDLPIGELDIKPFRDDMRVEEDTTLMSVDITGKDVILIDDVLYT
                     GRTIRAAIDNLVSLGRPARVSLAVLVDRGHRELPIRADYVGKNIPTSSVEEIVVEVVE
                     VDGRDRVSIIDPT"
     gene            24262..25521
                     /locus_tag="ACGEUI_000422"
     CDS             24262..25521
                     /locus_tag="ACGEUI_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFF8165284.1"
                     /translation="MKDVIYDVEEVPKAGMLVGLSFQHLFAMFGATVLVPILVGIDPS
                     VALLSSGLGTLAHLSVTKFKIPAYMGSSFAYIAAMQLLMKTNGIGAVAQGAMTGGLVY
                     LIVALIVKAIGNDWIDNILPPIVVGPIVMVIGLSLASTAVNDVMLKNGNYNLTYLVIG
                     LVTLLSVIFFNIYGKGIVAIVPLLLGLLVGYVVALLVGVLTGQEIVDFTNVAQAKWFS
                     IPSVEIPFLTYGVKFYPSAILTMAPIAFVTMTEHFGHIMVLNSLTKRDYFKDPGLEKT
                     LTGDGFAQIIAGFLGAPPVTSYGENIGVMALNKIFSVYVIAGAAVIAALLSFIGKVSA
                     LIQSIPTPVIGGISVALFGVIASSGLKILIESKVDMDNKKNLLIASVILVSGIGGLML
                     QVNGLQISGVAFSTLLGIILYQVLPEK"
     gene            25582..26517
                     /locus_tag="ACGEUI_000423"
     CDS             25582..26517
                     /locus_tag="ACGEUI_000423"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFF8165285.1"
                     /translation="MSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDH
                     QKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALG
                     TDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGL
                     KIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELD
                     VLMLLRVQHERHDGHQSFSKEGYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADS
                     LVEAPKARIVSQMANGVFVRMAIIEAILNGRNKNS"
     gene            26561..27643
                     /locus_tag="ACGEUI_000424"
     CDS             26561..27643
                     /locus_tag="ACGEUI_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFF8165286.1"
                     /translation="MTKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLIGNYGINRDDYESISPTCKGVVVSEVSRLASNWRKQMTLDAFLK
                     IKGIPGISGIDTRALTKIIRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVST
                     KTAYPAPGIGKNIVLVDFGLKHSILREFSKRQCNITVVPFNITAEEVLQLNPDGLMLS
                     NGPGNPEDLPEALDMIRGVQGKIPIFGICMGHQLFSLANGAKTCKMTFGHRGFNHAVR
                     EIATGRIDFTSQNHGYAVERSSLPDTLMVTHEDINDKTVEGVKHRDFPAFSVQFHPDA
                     APGPHDASYLFDEFLEMIDSWRCTSK"
     gene            27869..31045
                     /gene="carB"
                     /locus_tag="ACGEUI_000425"
     CDS             27869..31045
                     /gene="carB"
                     /locus_tag="ACGEUI_000425"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009568558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFF8165287.1"
                     /translation="MPKRKDIQKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYKV
                     ILVNSNPATIMTDKEIADKVYIEPLTLEFVNRIIRKERPDAILPTLGGQTGLNMAMAL
                     SKAGILDDLEIELLGTKLSAIDQAEDRDLFKQLMQELDQPIPESTIVKTVDEAVTFAR
                     DIGYPVIVRPAFTLGGTGGGICSSEEELCEITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLKIIRALK
                     IEGGCNVQLALDPYSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     INPITGTTYAMFEPALDYVVAKIPRFPFDKFEHGERQLGTQMKATGEVMAIGRNLEES
                     LLKACRSLEIGVCHNEMTSLSNISDEELVTKVIKAQDDRLFYLSEAIRRGYSIEELES
                     LTKIDLFFLDKLLHIVEIEQELQMHVDHLESLKKAKRYGFSDQKIAEIWQKDESDIRA
                     MRHSHSLYPVYKMVDTCAAEFDAKTPYFYSTYELENESVQSNKESILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQKAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAQALSEAGVTILGTQVEDLDRAEDRDLFEKALKELGIPQP
                     QGQTATNEEEALEAAKKIGFPVLVRPSYVLGGRAMEIVENKEDLREYIRTAVKASPEH
                     PILVDSYIFGKECEVDAISDGKSVLIPGIMEHIERAGVHSGDSMAVYPPQQLSKQIQE
                     TIAEYTKRLAIGLNCIGMMNVQFVIKNEQVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKLILGQTLKDLGYEDGLYPQSPLVHIKAPVFSFTKLAQVDSLLGPEMKSTGEVMGSD
                     TSLEKALYKAFEANNSHLSEFGQIVFTIADDSKAEALSLARRFKAIGYQIMATQGTAA
                     YFAEQGLSACLVGKIGDAANDIPTLVRHGHVQAIVNTVGIKRTADKDGQMIRSSAIEQ
                     GVPLFTALDTAKAMLTVLESRCFNIEAI"
     gene            31252..32523
                     /locus_tag="ACGEUI_000426"
     CDS             31252..32523
                     /locus_tag="ACGEUI_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFF8165288.1"
                     /translation="MSKRGKIKITTKTKLITASVITLVLIITGIVLWKQQRNTLTADI
                     AKEPYSTVSVTEGSIASSTLLSGTVKALSEEYIYFDANKGNDATVTVKVGDQVTQGQQ
                     LVQYNTTTAQSAYDTAVRSLNKIGRQINHLKTYGVPAVSTETNRDEATGEETTTTVQP
                     SAQQNANYKQQLQDLNDAYADAQAEVNKAQIALNDTVVISSVSGTVVEVNNDIDPSSK
                     NSQTLVHVATEGQLQVKGTLTEYDLANVKVGQSVKIKSKVYSNQEWTGKISYVSNYPT
                     ESNAGSTTPAGSTGAGSSTGATYDYKIDIISPLNQLKQGFTVSVEVVNEAKQALVPLT
                     AVIKKDKKHYVWTYDDATGKAKKVEVTLGNADAQQQEIHKGVAVGDIVIANPDKNIKP
                     DKKLEGVISIGTNTKPEKDSQSKNKKSGVDK"
     gene            32523..33233
                     /locus_tag="ACGEUI_000427"
     CDS             32523..33233
                     /locus_tag="ACGEUI_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8165289.1"
                     /translation="MSHEKKQLMQLSNIVKSYQNGDQVLKVLKGINLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDRPTSGDYTLHNTKIEILNDRELAKVRNDEIGFVFQQFFLL
                     AKLTALQNVELPLIYAGVNVSKRREQAKQFLEKVGLGRRIKHLPSELSGGQKQRVAIA
                     RALVNDPSIILADEPTGALDTKTGQQIMELLTELNKEGKTIIMVTHEPEIADFATRKI
                     IIRDGDITTDTTASVVID"
     gene            33245..34465
                     /locus_tag="ACGEUI_000428"
     CDS             33245..34465
                     /locus_tag="ACGEUI_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8165290.1"
                     /translation="MENWKFALSSIWGHKMRSILTMLGIIIGVAAVVIIMGLGNAMKN
                     SVTSTFSSKQKDIQLYFQEKGEEEDLYAGLHTHENNHEVKPEWLEQIVKDIDGIDSYY
                     FTNSATSTISYEKKKVDNASIIGVSKDYFNIKNYDIVAGRTLTDNDYSNFSRIILLDT
                     VLADDLFGKGNYKSALNKVVSLSDKDYLVIGVYKTDQTPVSFDGLSGGAVMANTQVAS
                     EFGTKEIGSIYIHVNDIQNSMNLGNQAADMLTNISHIKDGQYAVPDNSKIVEEINSQF
                     SIMTTVIGSIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRLKILSQFLIES
                     VVLTVLGGLIGLLLAQLSVGALGNAMTLKGACISLDVALIAVLFSASIGVFFGMLPAN
                     KASKLDPIEALRYE"
     gene            34719..36452
                     /locus_tag="ACGEUI_000429"
     CDS             34719..36452
                     /locus_tag="ACGEUI_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFF8165291.1"
                     /translation="MTFLSDLISLMTKIRLSWVIKAGIFQLLFVTIANIVLSEFFYFI
                     LDVTGQYHLDKDNVVTFLKNPIALALLGAYLFLLAAFIHLEFFALYRIIADQEISFYL
                     FRKQFSYYLRGLWKTFSGYQLLLFLLYILLTIPVLHIGLSSVITQKLYLPEFIVGELS
                     KITSTKYLLYGSLILVFYLNLRLVYFLPLIAINHRTVAQAWRESWQKTKKKHVLLWMK
                     LFAINGLTIVVLSLAISMILIFVDMFNPKGNNIIVQLGALTFTWELIFFTTIFFKLCS
                     AMILKEAIEPQKQYDEPRRSNKAYVVIFIVVTVGFAYQSLERLTFFDTSHSKTVIAHR
                     GLVSAGVENSLEALEGAKKAGSDYVELDLILTKDNHFVVSHDNRLKRLAGVNKTIRNL
                     TLKEVEHLTSHQGHFSGRFVSFDTFYQKAKKLNMPLLIELKPIGTEPGNYVDLFLETY
                     HRLGISKDNKVMSLDLEVIEAIKKKNPSITTGYIIPIQFGFFGDEFVDFYVIEDFSYR
                     SYLSSQAFWNNKEIYVWTINDPKRIEHYLLKPIQGIITDQPALTNQLIKDLKQDNSYF
                     SRLVRIISSLY"
     gene            36579..36851
                     /gene="rpsP"
                     /locus_tag="ACGEUI_000430"
     CDS             36579..36851
                     /gene="rpsP"
                     /locus_tag="ACGEUI_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFF8165292.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQITIKEDRVLEWLSKGAQPSDTVRNILSKAGVMAKFHDQKFSK"
     gene            36861..37100
                     /locus_tag="ACGEUI_000431"
     CDS             36861..37100
                     /locus_tag="ACGEUI_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFF8165293.1"
                     /translation="MDTIENLIIAIVKPLISQPDNLTIKIEDTPDFLEYHLDLDAQDI
                     GRVIGKKGRTITAIRSIVYSVPTLGKKVRLVIDEK"
     gene            38011..41037
                     /locus_tag="ACGEUI_000432"
     CDS             38011..41037
                     /locus_tag="ACGEUI_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFF8165294.1"
                     /translation="MKKHLKTVALTLTTVSVVTHNQEVFSLVKEPILKQTQASSSISG
                     ADYAESSGKSKLKINETSGPVDDTVTDLFSDKRTTPEKIKDNLAKGPREQELKAVTEN
                     TESEKQITSGSQLEQSKESLSLNKTVPSTSNWEICDFITKGNTLVGLSKSGVEKLSQT
                     DHLVLPSQAADGTQLIQVASFAFTPDKKTAIAEYTSRAGENGEISQLDVDGKEIINEG
                     EVFNSYLLKKVTIPTGYKHIGQDAFVDNKNIAEVNLPESLETISDYAFAHLALKQIDL
                     PDNLKAIGELAFFDNQITGKLSLPRQLMRLAERAFKSNHIKTIEFRGNSLKVIGEASF
                     QDNDLSQLMLPDGLEKIESEAFTGNPGDDHYNNRVVLWTKSGKNPSGLATENTYVNPD
                     KSLWQESPEIDYTKWLEEDFTYQKNSVTGFSNKGLQKVKRNKNLEIPKQHNGVTITEI
                     GDNAFRNVDFQNKTLRKYDLEEVKLPSTIRKIGAFAFQSNNLKSFEASDDLEEIKEGA
                     FMNNRIETLELKDKLVTIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGANNLIFMGS
                     KVKTLGEMAFLSNRLEHLDLSEQKQLTEIPVQAFSDNALKEVLLPASLKTIREEAFKK
                     NHLKQLEVASALSHIAFNALDDNDGDEQFDNKVVVKTHHNSYALADGEHFIVDPDKLS
                     STIVDLEKILKLIEGLDYSTLRQTTQTQFRDMTTAGKALLSKSNLRQGEKQKFLQEAQ
                     FFLGRVDLDKAIAKAEKALVTKKATKNGQLLERSINKAVLAYNNSAIKKANVKRLEKE
                     LDLLTGLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSDT
                     IGEGQKDAYGNPILNVDEDNEGYHALAVATLADYEGLDIKTILNSKLSQLTSIRQVPT
                     AAYHRAGIFQAIQNAAAEAEQLLPKPGTHSEKSSSSESANSKDRGLQSNPKTNRGRHS
                     AILPRTGSKGSFVYGILGYTSVALLSLITAIKKKKY"
     gene            41057..41458
                     /locus_tag="ACGEUI_000433"
     CDS             41057..41458
                     /locus_tag="ACGEUI_000433"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFF8165295.1"
                     /translation="MITQEMKDLINNQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGAYYEAAKKWAQGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEINSESN"
     gene            complement(41638..42513)
                     /locus_tag="ACGEUI_000434"
     CDS             complement(41638..42513)
                     /locus_tag="ACGEUI_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8165296.1"
                     /translation="MPASKKVTIIFILNLSFSLIEFIFGTLFFSGAILADAVHDFGDA
                     IAIGISAILERKAVKKESPNFSLGYKRFSLLGALTTNLILISGSLLVMIETIPKLWHP
                     TIVNYDGMFVLAIFAIIINGFASFIIHSNQTKNEEILSLHFLEDILGWLAIIILSLIL
                     KWKPWYILDPLLSIAIASFILSKALPKLVATANIFLDGVPDSIDYCTLHHELSQLPHI
                     VSVNQLNVWSMDGIDHRATIHCCLRESTTEKHCKKSIRLICQRYNINSVTVEIDTSLN
                     EHQHHCSSLSSIEVN"
     gene            42649..43170
                     /locus_tag="ACGEUI_000435"
     CDS             42649..43170
                     /locus_tag="ACGEUI_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8165297.1"
                     /translation="MTKDRQIKKTKTAIYSAFIALLQKKEYSKITVRDMITLANVGRS
                     TFYAHYESKEMLLKELCEELFHHLFRQKRNVTFEDYLVHILKHFEQNKDSIATLLLSN
                     DPYFLLRFKNELEHDVYPNLRCKYIDKTTIPEVFLKQFVLSSFIETLKWWLHQRQRMS
                     ANELLKYYLELIK"
     gene            43385..43903
                     /gene="rimM"
                     /locus_tag="ACGEUI_000436"
     CDS             43385..43903
                     /gene="rimM"
                     /locus_tag="ACGEUI_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFF8165298.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVSDFAEERFKKGSQLALFDDK
                     DRFVQEVTIVSHRKQKHFDIIKFKDMYHINAIEKYKGYTLKVSKDNQGDLQEGEFYYH
                     QIIGMAVYEKDVLIGHVKEILQPGANDVWIVKRQGKRDLLLPYIPPVVLNVDVPNKRV
                     DVELMEGLDDED"
     gene            43893..44624
                     /gene="trmD"
                     /locus_tag="ACGEUI_000437"
     CDS             43893..44624
                     /gene="trmD"
                     /locus_tag="ACGEUI_000437"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFF8165299.1"
                     /translation="MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKT
                     RHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRIILLDPAGKPFTQAYAEELALEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLKKTYLRRPDLL
                     EHYNFSEEERKLLDKIKEALDQGED"
     gene            44624..45616
                     /locus_tag="ACGEUI_000438"
     CDS             44624..45616
                     /locus_tag="ACGEUI_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8165300.1"
                     /translation="MKDKAYDITIIGGGPIGLFAAFYAGLRGVTVKIIESLSELGGQP
                     AILYPEKMIYDIPAYPSLTGVELTENLIKQLSRFEDRTTICLKEEVLTFDKVKGGFSI
                     RTNKAEHFSKAIIIACGNGAFAPRTLGLESEENFADHNLFYNVHQLDQFAGQKVVICG
                     GGDSAVDWALALEDIAESVTVVHRRDAFRAHEHSVELLKASTVNLLTPYVPKALKGIG
                     NLAEKLVIQKVKEDEVLELELDSLIVSFGFSTSNKNLKNWNLDYKRSSITVSPLFQTS
                     QEGIFAIGDAAAYNGKVDLIATGFGEAPTAVNQAINYIYPDRDNRVVHSTSLID"
     gene            45794..46852
                     /locus_tag="ACGEUI_000439"
     CDS             45794..46852
                     /locus_tag="ACGEUI_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFF8165301.1"
                     /translation="MTTTDKETFSSFMNKVLAGTAIAIVVALIPNAILATFLKPLLPN
                     MAAAEFLHIVQVFQFFTPIMAGFLIGQQFKFNPMQQLAVGGAAYIGSGAWAYTEVIQK
                     GVATGTFQLRGIGDLINMMITASLAVLAVKYFGNKFGSLTIILLPITIGTGVGYIGWK
                     FLPYVSYVTTLIGQGINSFTTLQPILMSILIAVAFSLIIVSPISTVAIGLAIGLNGMA
                     AGAASMGIASTAAVLVWATLKVNKSGVPIAIALGAMKMMMPNFLKHPIMAIPMVFTAA
                     ISSLTVPLFNLVGTPASSGFGLVGAVGPIASLAGGSSILIIILAWIIVPFAVAFAAHK
                     VSKDILKLYKEDIFVFEG"
     gene            46865..47788
                     /locus_tag="ACGEUI_000440"
     CDS             46865..47788
                     /locus_tag="ACGEUI_000440"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFF8165302.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGL
                     VVTGDVEETVKLPIMKPTEATQEADLIILFTKAMQLPQMLQDIKGIIGKETKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLEGPGRAHLQGVGALNLQSMDPSNQEAGHQV
                     ADLLNEANLNATYDENVVPNIWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEI
                     IHEFVIVGQAEGVELNEEEITQYVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAV
                     NTKGEKLGINTPYCRMITELVHAKEAVLNIQ"
     gene            48044..48757
                     /locus_tag="ACGEUI_000441"
     CDS             48044..48757
                     /locus_tag="ACGEUI_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8165303.1"
                     /translation="MAKITEENYVSLEDLMQLLNSSESTIRRDLGELEQEGRLHRVHG
                     GAELFHSLQEELSNQEKSVKNSHIKKAIAQRASQLIYDNDVIFIDAGTTTEFLLPFLQ
                     AKNLTVVTNSIHHAARLVELSIETIIVGGYVKQTTDASIGNVALEQIRQMNFDKAFLG
                     MNGVDDSYLTTPDMEEAVIKKAVLSNAKLAYILVDGTKIGQVSFVKVAPINDVTIITL
                     GGSASILKQIKEKAKVIEL"
     gene            48754..49665
                     /gene="pfkB"
                     /locus_tag="ACGEUI_000442"
     CDS             48754..49665
                     /gene="pfkB"
                     /locus_tag="ACGEUI_000442"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFF8165304.1"
                     /translation="MIYTVTLNPSIDFIVRIDQINLGSVNRMASDDKFAGGKGINVSR
                     ILQRLDIASTATGFLGGFTGRFIEESLSAEGVKTDFVKGDQDTRINVKIKSQEETELN
                     GQGPIISQEQLEDLKTKLSQLTAEDTVVFAGSAPANLGNAVYKELLPLVRQSGAQVVC
                     DFEGQTLIDALAYNPLLVKPNNHELEAIFGTILTSLDDVETYARRLLEMGAQNVIISM
                     AGDGALLVTKEATYFAKPIKGEVKNSVGAGDSMVAGFTGEFMKSQNPIEALKWGVACG
                     TATAFSDDLATIAFIKETYHKVEVEKR"
     gene            49662..51608
                     /locus_tag="ACGEUI_000443"
     CDS             49662..51608
                     /locus_tag="ACGEUI_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFF8165305.1"
                     /translation="MKIQDLLRKDIMILDLQAISKEVAIDEMITKLVEKDIVHDFDVF
                     KKSIMTREEQTSTGLGDGIAMPHSKNIVVDKPAVLFAKSNKGVDYKALDGQPTDLFFM
                     IAAPQGANDTHLAALAELSQYLLKDGFADKLRAAATPEAVIAVFDEASTAKEEVVAPT
                     SGQDFIVAVTACPTGIAHTYMAEEALKKQAAEMGVAIKVETNGASGVANRLTAEDIQR
                     AKGVIVAADKAVEMDRFDGKQFIARPVADGIKKSQELISLILNNEGNTYHAKNGKSET
                     AVSTEKTSLGGAFYKHLMGGVSQMLPFVIGGGIMIALAFLLDNMLGVPNDQLGSLGSY
                     HEIAAIFMNIGGAAFSFMLPVLAGYIAYSIAEKPGLVAGFVAGAIASNGLAFGKVPFA
                     AGGEVSLGLTGVPSGFLGALVGGFLAGGVILALRKLLAGLPRSLEGVKSILLYPLLGV
                     LVTGFLMLFVNIPMAAINTALNDFLQGLSGSSAVLMGLLVGGMMAVDMGGPVNKAAYV
                     FGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFTKEERESGLTNIVMG
                     LSFITEGAIPFGAADPARAIPSFIAGSALTGALVGLAGIKLMAPHGGIFVIALTSNPI
                     LYLVFVVIGALVSGILFGALRKKA"
     gene            51701..52300
                     /locus_tag="ACGEUI_000444"
     CDS             51701..52300
                     /locus_tag="ACGEUI_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8165306.1"
                     /translation="MKVKKRRRRAKSSVNRLVLGLVLLNLIVSMWTLKLGNQRLAPYA
                     DHETLTFVRKISHAAQSVAQKKQLYSSVMMAQAILESNNGKSQLSQKPYYNFFGIKGS
                     YKERSVIFPTLEDDGQGNLYQIDAAFRSYGSLTACFLDYARVLNDPLYDKTHKKFWSH
                     YQDATATLTGTYATDTTYHTKLNELIEWYQLTNFDGLMK"
     gene            52452..53159
                     /locus_tag="ACGEUI_000445"
     CDS             52452..53159
                     /locus_tag="ACGEUI_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8165307.1"
                     /translation="MTKKKGKLVLISLFVLAACLGAYSAMRQSHKTSNVSAETIASSS
                     TRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSLSQAPYYNFFGIKGAYNG
                     SSVTMSTWEDDGNGNTYTIDQAFRAYPSIADSLNDYADLLSSSTYIGARKSNTLSYQD
                     ATAALTGLYATDTSYNLKLNNIIATYGLTAYDVANSSAQETGLATSGYVWNEYRRNYT
                     DAETLAVDEAWAKRMTY"
     gene            complement(53216..53551)
                     /locus_tag="ACGEUI_000446"
                     /pseudo
     CDS             complement(53216..53551)
                     /locus_tag="ACGEUI_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(53731..>54375)
                     /gene="spej"
                     /locus_tag="ACGEUI_000447"
     CDS             complement(53731..>54375)
                     /gene="spej"
                     /locus_tag="ACGEUI_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeJ"
                     /protein_id="HFF8165308.1"
                     /translation="GSVKSDSENIKDVKLQLNYAYEIIPVDYTNCNIDYLTTHDFYID
                     ISSYKKKNFSVDSEVESYITTKFTKNQKVNIFGLPYIFTRYDVYYIYGGVTPSVNSNS
                     ENSKIVGNLLIDGVQQKTLINPIKIDKPIFTIQEFDFKIRQYLMQTYKIYDPNSPYIK
                     GQLEIAINGNKHESFNLYDATSSSTRSDIFKKYKDNKTINMKDFSHFDIYLWTK"
BASE COUNT        17361 a         8917 c        11127 g        16970 t
ORIGIN      
        1 atgcaacaat ctatgactaa agttaaatat ctttggaaag aaaataataa ctatatttat
       61 tcgacttttt tgacattact tctgacagtt attttatatg ccaatagttt aaatctacct
      121 ttaaaattta ttagtcttct ttttgtggtg agcactttac tgatagtgtt tttaccaaaa
      181 aagttagagg ttgctacgat catttttatt ttagtatttg gaacgatatc tgctatcata
      241 tcacctataa atgatattcc tgatgaatat gttcattatt caaggacagt ttatatctca
      301 gaaggtgata ttaatcttac caacaataac aaaaagttaa gaatatccaa agatgttgat
      361 aagcttatta aacagtcagg aaaaacattt ataacatcaa acttgaaagc aactaaacac
      421 agcacaagag agtatagcta tccttacatc aaaggaacta atgcatatta tagtttttct
      481 tatataccac aagctttggg tattttagta ggaaatgcgc tggatttacc tatactttta
      541 acatattatt tcggaagatt gtgcaacctt attagctacg ctatgcttgc ttttatagct
      601 atcaagttgt ctggttcttt taaacaagtg atagctgttg ttactttact acctatgaac
      661 atttatttag cagcatcttt taatcaagac ggctttgcta tcggtttagt tttagtaaca
      721 attggattgt ttattaactt gctctcatca aaagataaat ctaactataa taccaaattc
      781 ttcctttatt tagttctatg tgggttacta gttctttcaa aattcacata ctttttatta
      841 gtatgccttc cattatttat tcctaatgaa aaattcggaa aaaataccaa gttagtcatc
      901 ttgaaaaaat tgggaggact attactcatt tttctttttg ctgcaatgtg gtttagactt
      961 tatggacagg ttaaaacccc atatgtcgcg gattttctaa aagaagtaaa cgttagccag
     1021 caagttaaaa atatgctaga atcccctata gtctatagca gtattattat tagacatatg
     1081 gtaattaatc tgattaatat gaataatatt tttcagtttg gcgcattaag ctatggtata
     1141 acgaaccttt tcccgcttta tgtgtgtttc ttcttttttg tttatattag taatgctagt
     1201 aaaataacta ttaatattgt agaaaaaatg ggtataatct ttgttatttc agcgattata
     1261 ggtgcaactg tattagctat gtacctgact tggacaccag tagggtcctc aactgtctta
     1321 ggtgttcaaa gtcgttatct gataggaatt atcccattag tgttgcttct ttttagtagt
     1381 caacagcaaa agttcaaaca aatagaggat atcctttctg acaaactggc tattcatgtt
     1441 tcgcttttgt ttattcttgc aatgttaatg agtactattt ttagatatta tcattaaact
     1501 cttaattaga ggctttaaaa aacttagctt gaataagtat tttaaagcct tttatgctat
     1561 aattaaattg agaaattatt gaggtggcct atgaagtacg ataacttgtt agatcgattt
     1621 ataaaatatg tcaaagtgaa tacaagaagc gttccagaca gtgagactac tcctagtacg
     1681 gagagtcaag aagcatttgc cttaacgatt ttaaagcctg aaatggaggc aattggtttg
     1741 caagatgttc attataatcc agttaatggc tatcttattg gaactctgcc tgccaataat
     1801 ccaaccttga cgcgtaaaat tggttttata gcccatatgg atacggctga tttcaatgct
     1861 gaaaatgtta atccgcagat tattgacaat tatcaaggag gggacattac acttggcagt
     1921 tctaattata agttggatcc aaaagcattt ccaaatctta ataattatat cggtcaaaca
     1981 ttgattacca ctgatgggac tacactttta ggggcagatg ataaatctgg tattgctgaa
     2041 atcatgacag caatagaatt cttgacatca caaccacaaa tagagcactg tgacattaaa
     2101 gtggcttttg gccctgatga agagattggg gtcggagctg ataagtttga ggtggctgat
     2161 tttgaggtgg attttgctta tactatggat ggcggtcctt taggcgaatt gcaatatgaa
     2221 actttcagcg ctgcagctct agaagtgact tttttaggca gaaatgttca tcctggtacg
     2281 gctaaggatc aaatgattaa tgcccttgag ttagcaatag attttcatga gaagctacct
     2341 gctaaagatc ggcctgaata cacagatggt tatcaaggtt tctatcactt aacgggacta
     2401 actggaacag ttgaagaggc acgcgcatct tacattattc gtgattttga agaagcatca
     2461 tttgaagcta gaaaagtgaa agttgaaaat atagctcaat cgatgaatgc tcagctagga
     2521 acaaaacgtg tcttggtaga gcttaatgac caatactaca atatgaaaaa agttatcgaa
     2581 aaagatatga ccgctattga gttagctaaa gaagtaatgg aagagcttgc tatcaagcct
     2641 gtaatcgaac ccatacgtgg aggaacagat ggctctaaaa tttcatttat gggtattccc
     2701 actcctaata tttttgcagg gggtgagaat atgcacgggc gttttgagtt tgttagctta
     2761 caaacgatgg agagagctgt cgatgtcatt attggacttg tttgcaaagc ctaattatca
     2821 tggaattgat atttagtgga aggggcttat aaaaatgatt aaactatttg gtaaaataag
     2881 gtatcactgg cagccagagt tgtcttggtc tattatttat tggtctattg cgtttgcacc
     2941 tatttttgta ggtttatctt tgttgtatga gcgcactgaa atacctagtc gggtttttat
     3001 tctatttgct atttttgctg tcctagttgg cattggatta catcgttatt tcattattga
     3061 aaataatggt attttaagaa tcgtatcttt taaattgttt ggtccacgta agctacttat
     3121 ttccactatt actaagattg aggtgaccaa atcaacactt tgtctgcacg ttgaagataa
     3181 aagttatctc ttttatatgc gcaaatggcc taaaaaatat tttcttgatg ccttagcggt
     3241 taatccatac ttccaaggag aagttatatt aagtgataac tttattaaat tagactattt
     3301 tgaagtttat cagcatgata aaaaagccct tactcgaggg taagagcttt agttgggcaa
     3361 gatttgacag ctaaaatagc gtccttatct gaaggacaaa ttgactgact tgtttcagat
     3421 gagcttgaaa atgtgacgat accattgtca tgataatcaa aaagtgatga ataggtttgg
     3481 catagaccac aagcgatgca tttttctgga atgatagata ctttcataac tattattcta
     3541 atatgatttt gttaaattaa caagggggga cgatcatggc taaagaacca tgggaagaaa
     3601 aaattgttga tgatactata gggacacgaa cacgtaaatc aagaaatgct ttcattagca
     3661 cgccttggtt gactgcttta ttaagtgtat tctttgtcat cattgttgct atacttttta
     3721 ttttcttcta tacatcaaat agcggtagta atagacaagc tgaaacaaat ggtttttatg
     3781 gagcatccac tcataaaaaa acaaggaaag cttctaacgc taaaaaaaca tcaagtagtt
     3841 caacaactac agacacaaca ccttctagcg aagaaacact tgcttctagt gaaggaactg
     3901 gcgaaaccct tactgtattg gcaggtgagg gggcagcttc tattgcagct cgtgcaggta
     3961 tttctgtgga gcagttacaa gcacttaatc cagagcacat gactcaagga tattggtatg
     4021 ccaatccagg agatcaagtc actattaaat aattgaagag gaaaaaatga aagcaattaa
     4081 aattgcgatt gatgggccag cttcaagtgg aaaaagtaca gtagccaaga ttattgcaaa
     4141 aaatcttggc tatacttatc tagatactgg tgctatgtat cgctcagcaa cttatatcgc
     4201 actgacacat ggctatacgg gtaaagaagt agcccttatc ttagaggaac ttgagaaaaa
     4261 tcctattttc tttaaaaaag caaaagatgg ctcccaatta gtttttcttg gggatgagga
     4321 cgtgacctta gccattaggc aaaatgatgt gactaacaat gtttcttgga tctcagcttt
     4381 gccagaaatt agagaagagc tggttcatca acaaagacgc attgcacaag ctggtggcat
     4441 tatcatggat ggtcgtgaca ttggaacggt tgttctccca gatgcggaac taaaaatctt
     4501 tttagtggct tctgtcgaag agcgggcaga gcgccgctat aaagaaaacc tagaaaaagg
     4561 aattgaatca gattttgaga cgctaaaaga agagattgca gctcgtgatt acaaggatag
     4621 tcatcgtaag gtttcacctc taaaagcagc tgaagatgcc cttatttttg ataccacagg
     4681 tgtttctatt gatggcgtgg tgcaatttat ccaagaaaaa gcagaaaaaa tagttgacat
     4741 gtcctagagc ctatgttaga atagtaacaa tgagaaaagt agaagtgaga gcttctcgcc
     4801 ttgcgactta ggttgtctgg ccctaacatg tcaaattcct atggaatatt atgtgtgcgg
     4861 gcttgattta tcaagtccgc tttcgttttt ctctaaaaaa ataaagaggt gaagatcata
     4921 gctaaaaagg atctattcat taatgatgaa attcgcgttc gcgaagttcg tctcgttggt
     4981 cttgaaggtg aacaattagg tattaaacca ttgtctgaag cgcaatcatt ggctgatgct
     5041 tcaaatgttg atttggtttt aatccagcca caagctgttc ctcctgttgc caaacttatg
     5101 gactatggaa agttcaaatt tgagtatcaa aagaaacaga aagaacaacg taaaaaacaa
     5161 agcgttgtga ctgtgaaaga agttcgtctc agtccagtta tcgataaagg tgactttgaa
     5221 acaaaacttc gtaacggtcg taaattcctt gaaaaaggaa ataaagttaa ggtttctatt
     5281 cgtttcaaag gacgtatgat tactcataaa gagattggtg ccaaggtact agctgacttt
     5341 gctgaagcga ctcaagatat tgctatcatt gagcaacgag caaaaatgga tggacgccaa
     5401 atgtttatgc aacttgcacc aatttcagac aagaagtaat tgtcacagtt tcattatatc
     5461 tttaagagga gaatactaaa atgccaaaac aaaaaacaca ccgcgcatca gctaaacgtt
     5521 ttaaacgtac aggttcaggc ggtttgaaac gcttccgcgc ctttacatca caccgtttcc
     5581 acggaaaaac taaaaaacaa cgtcgtcatc ttcgtaaagc tggtttggtt agctcaggag
     5641 atttcaaacg tatcaaagca atggttactg gtctttaatt ttaaaggcag tagacattta
     5701 cgagtattat aagaattatt tgaaggagaa ctattaatgg cacgtgttaa aggtggcgtt
     5761 gtttcacgca aacgtcgtaa acgtatttta aaattagcta aaggttacta tggagcaaaa
     5821 cacatcttgt tccgtactgc aaaagagcaa gtgatgaact cttactacta tgcataccgt
     5881 gatcgccgtc agaaaaaacg tgatttccgt aaactatgga tcactcgtat caatgcggct
     5941 gctcgtatga acggtttgtc atactcacaa ttgatgcacg gtttgaaact tgctgaaatc
     6001 gaagtaaacc gtaaaatgct tgctgattta gcagtagctg atgcagcagc tttcacagct
     6061 cttgctgatg cagccaaagc aaaacttggc aaataagaaa cgctatacct aaaaaataac
     6121 tttctattac ctatagaaag ttataaagaa acaaaaatga aggagacgat ggacgtcttc
     6181 tttttattat actcaatata ataaaaagca gtttcccatg ttttattacc agtaatgtgg
     6241 gatatttaga tggtagcaag gagttttata gctgatttgt tttctttagg tctaattagc
     6301 atattttgat tactgataaa cttgaatatc gccaataaaa aagaagcaaa attattattt
     6361 tgcttctagt cagatcatta aattgattat tctttatttt tggggttatc ctcttttagt
     6421 tcagaagttg ccgcgtcagc ttctacggga tttagttcta agtaagatgg tgctttaaaa
     6481 agtttctgag ttgatttatg accattttta ctgtataaac ttgttgactc tgatccgagt
     6541 tttgctgaaa tatctttcag ttgttttaac tgcttagtgt agataaactg gttaggatca
     6601 atagctttta gaccattttg agtatcaaag cgcaggagat cacccgtttg aatagcgtcg
     6661 ctagctgcta gttgttgagc aacagcagag cgaatttcct tagttttggc tatagtcatt
     6721 tcatcgggat tagtgatttc taaacctgtt tgcgtgttgt acagacgtcc actatagtta
     6781 gtgtattcag gagtcatata agtcccagat gttcgttgtg ccacaatctg gctattttga
     6841 ggtgataata aatcttgtcc taattgaaca aactgactag tgtcaatacc aagtatgtgg
     6901 agtaaagtag gaagagcatc gatttcaccg ccaaaggttt ctttgatact accattcgta
     6961 taacctggaa tatggatcat atacgggact cgttgtagca tagcattatc atattcagac
     7021 caagtttcag aatctttacc aagaagtgga gcaagactag aattacgaga atttgaaata
     7081 ccataatgat caccatataa aacaaaaata gaattgtcgt acaaaccagt ggctttcaag
     7141 taatcaaaaa aagatttaag ggcagcgtct aaataattgg cagtagcaaa gtaaccattg
     7201 atcgtttcat cgtctgtttt ggctaaagga aaaccttctt cactactttc gccttttaga
     7261 ctagtataag gatagtgatt actcacagtg ataaacttgg tataaaaagg ttgttgcatc
     7321 tgttcaagat attttatgga atccttaaac atgtacttat catttaaccc atattgaaaa
     7381 gagttcttac tattttgttt agagaagtag ctagaatcaa aaaaataatt atagccccat
     7441 tgtttataag cattattgcg attccagaaa gttccaacgt taccatgaaa gacagcgctg
     7501 gtatagccac ctttttgggc taaaatactt ggagtagcaa attgtgtatt ttcaccaccg
     7561 tagttcacca taaaagaacc actatttaaa ccaaataagg aattctccat catggtttca
     7621 gcatcagaag ttttacctgc tttaacctga tgaaaaaagt tagggaatgc taaggtagca
     7681 tttgagtgat aaagtgagtt gataaaaggc gttacctcat attctttatc tccttctttt
     7741 agtttataat ctattaagaa ttgttgaaag ctttctaaat gaagaacaat aatatttttg
     7801 ccttttccaa ttccaaaata ctgaggatca ggtgctgcat aatgtccttt gacataagtt
     7861 ttaacatcaa ttaattcttc tgcagtagct ccatttcgtt ctttttgagc ttgataggtt
     7921 tgattgccgc tatagagggt aaacgctggt agtcctaaag ctctcacaat ataagtattt
     7981 gaaaagccac gtgttaatag ttccggacga tcaatttctg ctaaaaaaag attaatagaa
     8041 agcaatagac tagataaagc ggtgatggca aatgctgcgc gcttgttaaa aggacgttga
     8101 tcatttttga tttttttagc gatagataaa ctaattagaa taataaaatc aagaatatag
     8161 ataatatccc agatgcgtaa aaggtttaaa gcagagtccc ctaatccagc ggagacttta
     8221 ctactagcta gcatggcact cactgtaata aagtctgaaa attctcggta atatatagaa
     8281 tttgatatca agagaatgtt aaggataata taaaccaccc aagaacagat ataaaaggct
     8341 cgggtattct taacgtaaag agcaaccccc aaaagtaaaa aagctagtgg gattggattg
     8401 ataatagtta gaaagacttg gtatatattt cccaaatcta gtgaaaagtc tgtatgatat
     8461 gcccacaggg tctttatcca gtaacaaaag agtagagtaa ttataaaacc caatcttgtg
     8521 ttgataaaac ctgtgataag tgttttaaat ttcttcacag tagcaacttc cttatcgttt
     8581 tttcctaaag atgtcatctc caaataaaat ctcagattaa attataccaa aaatgtgaag
     8641 ctaatgcttg ttgtaagttc aaatttagta ggatttttta tcagattttg ttataataaa
     8701 aactatgaat aaactctata ttgattcttt tgtcgaaaag aagctgacag caggggtaca
     8761 attattagat gaaaaagatt tttctaatat aaaggaaaaa aatcagttag ttcaactggt
     8821 gaccaagtcc aatcgtccca ttggaacagc atatatctct aagcaaaata aaggaattgg
     8881 ttggtatcta ggaccagaaa aaatcgactt gtctatctct tattttgtta gtctattttc
     8941 agttgctaag gccaaaagac aagattttgc tcaatcggac gagaccaatg cttatcgcct
     9001 ttttaatcaa gaaggagacg gttttggtgg agtaacaatt gatctttata aagattttgc
     9061 agtcttttca tggtacaatg cctttgtcta tgacaaaaaa gaaatgatta tggaggcttt
     9121 tcaacaggtt tttcctgaag tcaaaggagc ttatgaaaag tgtcgcttta aaggtccaga
     9181 tactgaaact gctcatctgt atggtgagct ggcacaagaa accttttcga ttttagaaaa
     9241 tggcatagcc tatcaagtct tcttgaatga gggcttaatg acaggtattt ttctagacca
     9301 gcatgatgtt agaagagcac tagtagatgg tttagctatg ggcaagtccc ttcttaacct
     9361 attttcctat acagctgcct tttctgtagc agctgctatg ggtggtgcta tcgagaccac
     9421 ttctgtggac ttggctaaac gatcgagaga attatcacta gctcattttg agcataacca
     9481 gttaaactta gcctcacatc attttgtggt gatggatgtt tttgaatact tcaaatatgc
     9541 caaacggaaa aaattaatct ttgatgtgat tgtgattgac ccaccaagct ttgctagaaa
     9601 taaaaaacaa actttctctg tgagcaggga ctaccacaaa ttaatcacag aggccttgga
     9661 catattgagt cctaagggaa ctattatagc ttcaacaaat gctgctaata tgactgttag
     9721 ccagttcaaa aagcaaatta tcaaaggatt tggaagtcgt cgtcctgagt ctatgactct
     9781 ccagcagctt ccgagtgact ttactatcaa taaagcagat gaaagaagta actatttaaa
     9841 ggtatttact ataaaggtac gagaatgaga attgtagcac cagtcatgcc aagacatttt
     9901 gacgaggctc aagccattga tatttctaaa tatgaagatg ttaatttaat tgaatggcga
     9961 gcagactttc ttcccaagga tgagattgtc gcagtagcac cagctatttt tgaaaaattt
    10021 gccggaaaag aaattatctt tacccttcgg accgttcaag aaggggggaa tattaccctt
    10081 tcaagtcagg agtatgttga tattatcaaa gaaatcaatg ctatttataa tccagactat
    10141 attgactttg agtattttac gcacaagtca gtatttcaag aaatgcttga ttttccaaat
    10201 cttattttgt cttatcacaa ctttgaagag actcctgaaa atttaatgga agctttttca
    10261 gaaatgacca agttggcacc gcgtgtggtt aaaattgctg tgatgcccca gagtgaacaa
    10321 gatgttctag atttgatgaa ctataccaga ggctttaaga cgctcaatcc tgagcaagaa
    10381 tttgcaacta tatctatggg gaaattagga agattgtctc gttttgcggg agatgtcatt
    10441 ggctcttcct ggacatacgt ttcattagat catgttagcg gtccaggtca agtgactctc
    10501 aatgatatga aacgtattat tgaggtctta gaaatggata tctctaacta gtgaatggtc
    10561 taaaaatatt atttctacaa tagttaagaa atgatgtttt tagattaatg tttttaaaat
    10621 aagtggaacc aaaaaggaga aaaattgcgt tatttaacag caggagaatc tcatgggcca
    10681 tccttaacag ctattattga aggtatacca gcaggattaa ctcttcatcc agcggatatt
    10741 gatcacgaat tacagcgacg tcaaggaggt tatggccgtg gtgccagaat gtctattgag
    10801 actgatcggg ttcaaatatc ctcaggagtt cgtcatggta aaacaacagg ggcccccatt
    10861 accttaacgg taattaataa ggaccaccaa aaatggctag atgtcatggc tgtaggagac
    10921 attgaagaga ctctcaagtt aaagcgtcgt gttaagcatc ctagaccagg acatgcggat
    10981 ttggtaggag gtattaaata tcattttaac gatttaagag atgctttaga gcgttcctcg
    11041 gctagagaaa caacgatgag agtggctgta ggcgctgttg ctaaacgtat cttagctgaa
    11101 cttggaattg atatgttaca tcatatctta atatttggtg gtataacgat cacaatacct
    11161 tctaagctct cttttagaga gctgcaagaa agggctctcc attcagaatt atctattgtt
    11221 aatccaaagc aagaagaaga aattaaaacg tatattgata agataaaaaa agaaggggat
    11281 accattggtg gtattatcga aacgatagtt caaggggtgc cagcaggtct tggatcttat
    11341 gtccaatggg ataaaaaatt agatgccaag ctggctcaag cagttctttc cattaatgct
    11401 tttaaagggg tggaatttgg agctgggttt gatatgggat tccaaaaggg ctctcaggta
    11461 atggatgaaa tcacctggac acctacccag ggctatggtc gtcaaacaaa ccacttaggt
    11521 ggctttgaag gtggcatgac cacaggacaa cctcttgttg ttaaaggggt tatgaagccc
    11581 attcctactt tgtataaacc actcatgtcg gtagatattg atagtcatga accttataaa
    11641 gcaacggtgg aacgctctga tccgacggcc ttaccagctg caggggttat catggaaaat
    11701 gtggtggcga cggtgcttgc caaagagatt ttagagactt tttcttctac taccatgtct
    11761 gaattgcaaa aagctttttc ggactatcgt gcctacgtca agcaatttta gatagcgaga
    11821 tactggcttt tatggtatca tacaggtatt aatgaacaaa aggagactgt catgtcacaa
    11881 gaaatttatg actatgctaa tcaacttgaa agagcagttc gtgctctgcc agaataccaa
    11941 aaagtgttag aagtaaaaga ggccattcaa gcagacgtta gtgctagcga actgtttgat
    12001 gagtttgtcg ctatgcaaga aaaaatccaa gggatgatgc aatcaggcca aatgccaacg
    12061 gctgaagaac aaacaagcat tcaagaactt agccaaaaga ttgaagctaa tgaccagcta
    12121 aaagcttatt ttgaagcgca gcaggcctta tctgtttaca tgagtgatat tgagcgtatt
    12181 gtatttgctc cgttgaaaga tttagtcaag taagccaatt aagataggaa aaaaacagat
    12241 tgctggcagc tcgtcaggat ctgtttttta gtgtttattt tctttgtatc agaaagttga
    12301 gcctctttct tatttgtgat atagtcaaat aaagtgacca aaatagcagg ccagtagtgg
    12361 ctttaatgaa ggcaagttgt ctgctaactg gctgtgctaa aacatcagta ttagaaagta
    12421 aaaggtgata aaaatggtta ttccttatga ttatattgtt attggtggag ggagtgcagg
    12481 tattgcttct gcgaacaggg cagccatgca tggggccaaa gtattgttag ctgaaggtaa
    12541 agagattggg ggaacctgtg ttaacctagg ctgcgttcct aaaaaagtca tgtggtatgg
    12601 cgctcaagta gcagatattt tagggactta cgctaaggat tatggatttg actttaaaga
    12661 gaaagcattt gattttaaac aattgaaagc aaatcgtcaa gcttatattg atcggattca
    12721 tgcttcttat gagcgaggct ttgagcagaa tggtgttgac cgtatttatg attatgctgt
    12781 tttcaaagat gctcatacgg tagaaattgc agggcagctt tataccgcac cgcatatttt
    12841 gattgccaca ggaggacatc ctgtttttcc agatattgag ggggcacaat acggtatcag
    12901 ctcggacggc ttttttgcct tagatgaggt accaaaacgt acggctgtgg ttggggcagg
    12961 ctatattgct gtggaacttg ctggtgtttt gcatgctttg ggatcgaaga cggatttgtt
    13021 tatccgtcac gatcgacctt taagaagttt tgacaaaacc attgttgatg tcttagttga
    13081 tgaaatggca gttaatgggc cgcgtttaca cactcatgca gaagtggcaa aggttgtcaa
    13141 aaatactgat gagtccttga ccctttacct caaagatggc caagaagttg aggttgacca
    13201 gctcatttgg gctatcggtc gcaagcctaa tctagaaggt tttagtcttg acaaaactgg
    13261 agtgacgtta aatgataaag gttatattga gacagatgcc tatgaaaaca cgtctgtcaa
    13321 aggtatttat gccgtgggag atgttaatgg gaaactggct ttaactccag ttgctgtggc
    13381 agctggacgt cgcctatcag agcgtctttt caatggcaaa acagacgaga aattggacta
    13441 ccaaaacgtt gctactgtga tttttagtca ccctgttatt ggctctgttg gcttgtcaga
    13501 agaagcagca gtcaaacagt atgggcaaga agcagttaaa acatatcaat cacgttttac
    13561 gtctatgttt actgctatca ctaaccatcg ccaaccttgt ctgatgaagt tggtaacagt
    13621 cggagatacc gaaaaaatag ttggtcttca cggaatcgga tacggggttg acgaaatgat
    13681 tcagggcttt gcagtagcca tcaaaatggg agcaaccaag gcggattttg ataacaccgt
    13741 tgctattcac ccaactggct cagaagaatt tgtcaccatg cgctaaaaaa tgtagcctaa
    13801 ggcaataaaa aatgatgtta acagtttttg aggaaagtct tctaaactgc tagcatcgtt
    13861 ttttgttagg tgattagggg aatatgtttt taaagatatt tgcgttgctc agacaatatt
    13921 gccttacctc ggagataaaa taaagtgacc ctgtaacaac aaagagttca tcagatgtta
    13981 cggaaccttg taaccagtct ctgtaatctt ttacctgtgg gtatttatca ggataatcct
    14041 ctaaaagatt tgcttcaggg aaatcaaaag tcgtgacaga tacttcaaaa gtatctagtt
    14101 gagccagcaa gtcggctaaa ggcttacgtt tgagacctgc aaagagaata tgaataggac
    14161 gtttgggaaa ttcttcttgc agtagagact ttaatttagc aatggaatct ggattgtgag
    14221 cgccgtctaa tagaagatta ggttgaataa attcggaccg gccaggccaa gttgttgcct
    14281 caattccttt ttgaatcgtt tttggagata ttttaggaaa ggattttcta agtaattgag
    14341 ctgtcataat ggctaaacag gcattcgctt tttgatgttg acctaatagt ttgagacgga
    14401 gtttggttaa attcaagtta tcgtgttgaa aggaaaacgt cttttctgaa ggattgagca
    14461 aaaaatcttt tcctaattga tataagcgtg aaccggcttt ttgggtaact tgttgaaaga
    14521 cttgactagc ctcctgctca agttgaccga taataacggg ttctttagct ttgataactt
    14581 ctgctttttg gtgagcaatt tctgctaggc tataacctaa acgttcttgg tgatcaaaac
    14641 taatagaagg gcatacgacg gccagagcat gaaaaacatt ggttgcgtcg gttttcccac
    14701 caatccctgc ctcaataatg gcaatatcaa caggtttaag attggcaaag tagcaaaaca
    14761 ttatgagcgt cactaactca aactcggtag gtctatccca atttgtttca agagacattt
    14821 tagtgagaat gggtctaatc acgctcacac attgcgccaa ttctaggtct gaaatgggct
    14881 ggccattaag gcaaatccga tcgtgaaaag agataataaa aggagatgta aaggttccga
    14941 cctgataacc agaagttgac aggatatgct gtaagtaggc tgtcacagag cccttgccgt
    15001 tagttccaac aacgtgaata gcagggaatt tgtcttgtgg ccggcctagt gcctcaagta
    15061 accaagccat tctggccaaa tcggtcttgc gaccattagc cttaaaggta tgaatccagg
    15121 aaatgctatc ataggtagtc attactattt cttgtccttc taacaatgct tttaagggtt
    15181 cgtaggaaga ccagtattag catcttcagc tccagttgca ttaggaattt gcgtcccttc
    15241 agtggctgat ggtgtctcag gagctgtagg agttgtctcg ttgtgagaag gtgtggtaga
    15301 agatgacgga agagttgtct tattatcatc tgtggtttct gaagtacctg tatggttttg
    15361 attaccctga gaagactctt tttcaataat cttgacaatg gtattactcg attctttggg
    15421 tgtatcatta gaggtcttat ctggtacagg ctgcagtgct cttgaaatgg taatggttgc
    15481 cacgatgata ctcaaaatac taccaataag tgcaatggat ttttggaaag ctgtaaaacc
    15541 tgttttaata tgctcggtag ggacacctcc tgatttacga ggtttttgtt gacgggaata
    15601 acgtgatgac atatttttct cctttaaaat catcgtaatg ccaagattaa ggaggaggaa
    15661 acagaagtcc tagctcctct ttaacacttc atctatcagt ataatctaga aaaaaagtga
    15721 agtcaacggt ttaccttatg tctaggacaa ggaagtcctt tgggcttatt gtaatacgaa
    15781 tattcttgat ttcattttag ctattttgtg atagaataaa gtattgagta aaaatgaagg
    15841 tgacacatga tttattttga taatgcagcc acaaccatac cttatggaga ggctcttaaa
    15901 acttaccaag aagtagcaac aaaaatatat ggcaatccct caagtctgca ccaattaggg
    15961 acaaatgcgt ctcgcatttt agaagcttcc cgtaaacaga ttgctggttt gttgggtgtt
    16021 aagtcagaag aaatcttttt tacgtctggt gggactgaaa gtgctaattg ggctattaag
    16081 ggaattgctt ttgaaaaaaa cgcttttggc aaacatatta ttatctcagc cattgaacac
    16141 cctgccgtga gtgaaagtgt caaatggctt ctcacacaag gattcgaagt ttcttatgct
    16201 ccagtgacaa cacagggagt tgttgatgtg aatgctttag ctgagctgat tagaccagac
    16261 accatcttaa tctcgattat ggctgttaat aatgaaatgg gagctatcca accgattaga
    16321 gccatttcaa acctattggc taaccaacca acgatcacct ttcacgttga tgctgttcaa
    16381 gccattggta aaataccact ctgtgattat atgactaatc gtgtggattt ggcttctttt
    16441 tcagggcaca aatttcatag tgttcgtggt gttggcttcc tttataaaaa agcaggcaag
    16501 cgactcaatc ctctcttgtc aggcggcggc caagagcagg agttacgttc aaccactgaa
    16561 aatgttgcag gaattgctag catggccaaa gcattaagaa tagtcactga aaaacaagtg
    16621 agcgtcttac ctaaactaac agcgatgcga gatgtgattt acaaatcttt gtccgcttac
    16681 ccagatgtga cagtattttc agctcaagaa ggctttgcgc ccaatatttt gacctttggg
    16741 attagagggg ttcgaggaga agttattgtc cacgcctttg aaaaatatga gatttatatt
    16801 tcgaccacaa gtgcatgttc gtccaaagca ggtaagccag caggaagcct agtggctatg
    16861 gggattcctg taaaaactgc ccagacagcc gttcgtatta gtctagacga tgacaacgat
    16921 atgggacaag tagagcagtt tttgaccata ttcaaacaga tttatgataa aacacagaaa
    16981 gttaggtaag attaggtaag atggactatt cagaaattat ggttcgccat ggtgaactat
    17041 caactaaagg gaagaatcgc atgcgattca ttaacaaact taaaaacaat attcaagacg
    17101 tactggctcc tttcccagcc attactgtcc gttcagatcg tgataggacc catgtgtctc
    17161 taaatggcac agattatcag cctattgtag aagctttaaa gctggtgttt ggtgttcaag
    17221 ctctatctcc tgtttataaa cttgaaaaaa gtgtccctct tttggtaaca gctgttcaag
    17281 atatcatgac ttctctttat cgtgatggac tgacgtttaa gattgccact aagcgtagtg
    17341 atcacgcgtt tgaattggat agtcgtgagc ttaacagtct tcttggtgga gctgtttttg
    17401 aggttttacc aaatatccaa gcccagatga aacatcccga tgtgactctc aaagtcgaaa
    17461 ttcgtgatga agcagcttac atttcttatg aagagattaa gggaacaggc gggttaccgg
    17521 taggaacgtc agggaaaggg atgctgatgt tatcgggcgg tattgattca ccagtagctg
    17581 ggtatttagc ccttaaacgt ggccttgata ttgaagttgt tcattttgct agccctccgt
    17641 acaccagtcc aggtgccttg gctaaagccc aagaccttac tcgtcgatta actcgttttg
    17701 gtggaaatat ccaatttatc gaggttcctt ttacagaaat tcaagaagaa attaaaaata
    17761 aggcaccaga ggcttacctt atgacattaa cccgccgctt tatgatgcgc atcacagatg
    17821 ccattcgtga gcaacgtaag ggtcttgtta ttgttaacgg tgagagccta ggtcaggttg
    17881 ctagtcaaac ccttgaaagc atgcaggcca ttaatgcggt aacatcaact ccaattatta
    17941 gaccagtcgt gacaatggat aaacttgaaa ttatcgagat ggctcaggcg attgatactt
    18001 ttgacatttc tattcaaccg ttcgaagatt gctgtactat ttttgcacca gaccgtccta
    18061 aaaccaaccc taaactaggt aatgctgaaa aatatgaaga acgctttgat attgatggtc
    18121 ttgtccagcg tgctgtgtca ggaattgttg tcacagaaat cacacctgaa atcgtcaatg
    18181 atgaggttga aaatctcatt gatgctttac tttaaaagaa aagatcaagt gtaaatcttt
    18241 tagtagtcag taatgatact attgaaaagc ctctataagg gcttttttgc tgcaatgaag
    18301 gaggaaaatt ttgaggcaag atgttataat agagaaaaag gagggtgtgt cttgattaaa
    18361 actatgcaat acaaaaaaac gatggcaaca gttgttgctc ttatcgtcgc acttttgcta
    18421 tttggcttga tttatgattt acttggggtt caaaaaaatg agctagcagc tcaaaaatct
    18481 gctcagccaa aggtcaaaac agcgcgtgtt gtagctaatg gcgacatttt aattcatgat
    18541 attctctaca tgagtgctag aaaagctgac gatacctatg atttcactcc ttattttgaa
    18601 tatgttaagg actggattag cggtgctgac ttagctattg gagattatga agggaccatt
    18661 agcccagatt atcccttagc aggctatccc ttattcaatg ctccagaaga gattgcaggt
    18721 gctctgaaaa atactggcta tgatgtagtc gatttagccc ataatcatat cttagattcc
    18781 caattagacg gtgcccttaa cactaaaaag gtttttcatc aattaggtat agacagtatt
    18841 ggcatctatg acaaggatcg ttcaaaagaa tccttcttaa ttaaaaatgt caatggtatc
    18901 aaaattgcaa ttttaggtta ttcttatggt tataacggca tggaggccac acttagccaa
    18961 gaagactatg agaaacacat gtctgattta gatgaagcta agataaaaaa agaacttcag
    19021 ctagctgaaa aaaaggctga cgtgaccatt gttatgccac aaatgggaac agaatatgcc
    19081 ttagaaccga cagcagaaca aaaagaactt taccacaaaa tgattgattg gggagctgac
    19141 gtcgtcctag gaggccatcc gcatgttatt gaaccttcag agacagttat aaaaggtagg
    19201 caaaagaaat tcattattta ctctatggga aattttattt caaatcagcg gcttgaaacc
    19261 gtagatgata tctggactga gcgtggccta ttgatggacc ttacttttga gaaaaaagat
    19321 aacaaaacaa aaattaaaac agttgaggcc catccaacca tggttttagc caaaggtaaa
    19381 ggcattgtgg gtaaagaagg ttttgaacta tacaattatc gaacaatggt tttagaagat
    19441 tttatcaaag gtggaaaata ccacgacaag attgatgagg aaaccaaagc aaaagtagca
    19501 cttgcctatc aggaaattaa tgatttagtt aacctgaaat ggtaacgttt ggtggtttag
    19561 catctaacga taaaaaatag agattaaact taatttttcc ttgataaaaa gcagttatgg
    19621 tgctaaaata gtaacgttga ctaaatgcac atcctgtgca accgcgcgaa aatcgttgtc
    19681 aagtagacca agtctcacga ttccaggcga gtcttcacag agggaaacct caaacaaaaa
    19741 ttttatagga ggtgcatcat gagcacatac gcaatcatca aaactggtgg aaaacaagtt
    19801 aaagttgaag taggtcaagc aatctacgtt gagaaaattg acgctgaagc tggcgcagaa
    19861 gtcactttta acgaagttgt tcttgtaggt ggtgacaaaa ctgtagttgg tactccagtt
    19921 gttgaaggag ctactgtcgt tggaactgtt gaaaaacaag gaaaacaaaa gaaagttgta
    19981 actttcaaat acaaacctaa aaaaggtagc caccgtaaac aaggtcatcg tcaaccttac
    20041 actaaagttg tcatcaacgc aatcaacgct taatttacta gcttatgatt aaagcaattt
    20101 ttactcgcca aaaaaatggt caactgagca gtgttacact gacaggtcat gctggtagtg
    20161 gtaaacatgg ctttgatatt gtttgtgcct ctgttagtac tctagccatt aactttgtta
    20221 attctcttga ggtgttagca gattgccaag cattagtaga tcttaatgat gttgaaggtg
    20281 ggtatatggc tattacaata ccaccacatg acaacaagga ggaagttcaa ttattatttg
    20341 aatcttttct tctaggaatg actagtttgg cgaaggattc ttcgaaattt gtcaacactc
    20401 aggtcatcta aatttaattt aagagaggaa acaacattat gttaaaaatg aatcttgcta
    20461 acttgcaact tttcgcccac aaaaaaggtg gaggttctac atcaaacgga cgtgactcac
    20521 aagctaaacg ccttggtgct aaagctgctg acggtcaaac tgtttcaggt ggatcaatcc
    20581 tttaccgtca acgtggaact catatctacc caggtgtaaa cgtaggccgt ggtggagatg
    20641 acacactttt tgctaaagtt gaaggtgttg tacgtttcga acgtaaagga cgcgataaaa
    20701 aacaagtttc tgtttaccca gtagctaaat aattgtttaa catcgcatta tataaggctt
    20761 tccaggtgtc tggaaagccg atttttttat tttgctactc atttgctacc catattctaa
    20821 aagccgatgt aggaagagag cttgtcagca gttttatctt ttgaggattt agtaacatga
    20881 gcgtagatat tcatggtagt ttggatagtt tcacgaccaa gtgtattgga ttttcttcta
    20941 tcctactaaa gattgtagtt gcttatttag agcaatagtt ctaattgtaa aaagtcagca
    21001 taataaaaca atgtcataag atgttgttat aacgggcttt atggtataat taaggatact
    21061 cattttagga aggaaagcaa tgaatattca acaattacgc tatgtggtag ccatcgctaa
    21121 taatggaact tttcgagaag ctgcctctaa attgtttgtc agtcagccta gcttatcagt
    21181 ttctatcaaa gatttagagg cggaattagg gtttcaaatt tttaatcgga ctacttcagg
    21241 aacagtcttg acaagtcaag gactagtttt ttatgagaaa gctttagaag tagtcaagtc
    21301 ttttgattct tttgaaaaaa ccttttctca agctgatctg gatcaaaatg aattttctat
    21361 tgctagccaa cattatgact ttttgccacc tcttattaca gctttttcgc aacaatacga
    21421 tggtcatcgt gtctttcgta tctttgaatc aacaactatt caaattttag atgaagttgc
    21481 tcaaggaaac agtgaaattg gtattattta cttgaatgta gacaatcaaa aaggtctctt
    21541 tcagcggatg gataagctgg gcttagaata tgtttctcta attcctttta ctactcatat
    21601 ttatctttca aaaacgcatc cattggctaa tcgcgaagcg ctttatttga acgatattca
    21661 aggattacct gcggtaaggt ttacccaaga aagggacgag tatttgtatt attctgaaaa
    21721 ttttgtggat actagtgaat gccctcgtat ttataatgtg tcagataggg caaccttgaa
    21781 cggtatttta gagagaacca atgcctttgc gacaggatca ggttttctcg atcatcggag
    21841 tgttaatggg attaaagtga tacctctagc tgatcatatt gacaatcaaa tgatttatgt
    21901 caaacgaaaa gataaaaacc tttctgttgc tggggccact tttgtgacta ttttaaaaga
    21961 ctattttgat aaaagaagga aggccttaac atgaaaaaac gattgtttgt gcttagcttg
    22021 atcctccttg tagctttgga tcaacttagt aaattttgga ttgtttctca tatagcgctt
    22081 ggagaagtga aaccctttat cccaggtatc gtcagcttga cttacttgca aaacaatggg
    22141 gctgcctttt ccatattgca ggaccagcaa tggttctttg ttgtcataac ggttttagtt
    22201 atcggttatg ctatttatta ccttgctact catccccatt taaatatctg gaaacaatta
    22261 gctctcttgc ttattatttc tggtggaatc gggaatttta ttgatcgttt gcgtttagct
    22321 tacgtgattg atatgattca tttagacttt gtggattttg ccatttttaa tgtggcagat
    22381 tcatacctta ccgttggtgt catattatta ttgatatgtt tatggaaaga agaggattat
    22441 ggaaattaat gtgataacat cggggcaacg cttggataag gcactagctg atttaagtcc
    22501 cctgtcacgt ggtcaagcca atgaccaaat taagcaaggc cttgttttag ttaatggtca
    22561 gcaaaaaaag gctaaatata cggttcaagc aggagatgtg atttgttttg aactccctaa
    22621 agaagaagtc ttagagtacc aagctcagaa tattcccttg gacattatct atgaagatga
    22681 tgctctcgcc attattaaca agccacaggg aatggtagtt cacccttcgg cgggacaccc
    22741 ttcaggaaca atggttaatg ccttgatgta tcatatcaag gatttgtcct caatcaatgg
    22801 tgtggttcgt ccaggaattg ttcatcggat tgataaggac acctccggtc tcttaatggt
    22861 agctaaaaca gatgcggctc atcaagcctt agcagaagaa ttgaaggcta aaaaatctct
    22921 cagaaaatac ctcgccattg tccatggaaa cctaccgaat gatcgcggga tgattgaagc
    22981 tcctataggt cgtagcgaaa aggaccgtaa aaaacaggct gttactgcaa aaggcaaaga
    23041 agcagtgaca cgttttacag tgctagaacg ttttggcgat tacagtctag tggaattaca
    23101 actggaaact ggtcgaacgc accaaattcg tgttcatatg gcctatattg gtcatcctgt
    23161 tgctggggac cctttatatg gaccacgtaa gacactatca ggtcatggcc aatttcttca
    23221 tgcaaaaaca ttgggattaa cgcatcctat gacaggaaaa gaaatgatat ttactgttga
    23281 agctcccgaa atttttcaaa aggtgttaaa gctgctaaga aaaaaataaa cagcgaggag
    23341 tagccattat ttttagtctt atatctttgt gtaaagtttt taacattata aaaaatacta
    23401 aaaagcattt acgataagcc aggaaatatg agaagtcttt tatgttattg ggatatgcaa
    23461 catatctgtt agagatcttt atttaaactc cttaaagaaa agagatattc caaatagatg
    23521 ctaggtattg tcttttttac ttgtttttct tttctttttt tgctatactg attgaaataa
    23581 aatattcctt taaaacagtc ctgtgaggct gtcaaggaga cggattaaaa acatgcatca
    23641 tgatacttaa ttgtgctata ttttgctgaa tctccttgtt tccaaggaga tctttttatt
    23701 tgaattttta gaggaggttt caacatgaaa actaaggaaa ttgttgatga cgtgaccatg
    23761 aaacgcgcca ttacccgtat tacttatgaa attattgaac gtaataaaca attagacaat
    23821 gtggttttgg caggcatcaa aacgagaggt gtttttctag ctcgtcgcat tcaagaaaga
    23881 ctgcatcaat tagaagggtt ggatttacca attggtgagt tagacatcaa gccttttaga
    23941 gatgacatga gagttgagga agacacaact ctgatgtcag tagatattac aggcaaggat
    24001 gtgattttga ttgatgatgt gctttatact ggtcgtacca tccgcgcagc catcgataac
    24061 ttagtgagtt taggtcgacc agcccgtgtt agtctagctg ttttggtgga tcgtggccat
    24121 cgggaattac cgattcgcgc ggattacgtt gggaaaaata ttccaacaag cagtgtagaa
    24181 gaaattgttg ttgaggttgt cgaagtcgat ggccgtgatc gtgttagcat tattgaccca
    24241 acataagaaa gaggttttac tgtgaaagat gtcatttatg atgtggaaga agtgccaaag
    24301 gcaggtatgt tagttggttt gtcctttcag cacttatttg ctatgtttgg tgcgactgtt
    24361 ctggttccca ttctagttgg aattgaccca tcagttgctt tgttatctag tggtttagga
    24421 acccttgccc acttatcagt gaccaaattt aaaattccag cctacatggg ctcaagtttt
    24481 gcttatatag cagctatgca gttactgatg aagacaaatg gtattggtgc tgtagctcaa
    24541 ggggctatga caggaggtct cgtttacctg attgtagctt tgattgttaa agctattggc
    24601 aatgattgga ttgataacat cttaccaccg attgtggttg gtccgattgt tatggtcatt
    24661 ggtctaagcc tagcttctac agctgttaat gatgtcatgc taaaaaatgg gaactataat
    24721 ctgacttatc ttgttattgg gttagtcacc ttattatcag ttattttttt caatatttat
    24781 ggtaagggaa ttgttgctat tgtaccgctt ttattagggc tattagttgg ttatgttgta
    24841 gcccttttag tgggtgttct caccggtcaa gaaattgttg attttaccaa tgtggctcag
    24901 gccaaatggt ttagtatccc atcagtggaa attcctttct taacctacgg ggttaaattc
    24961 tacccaagtg ccattttaac tatggcaccc attgcctttg ttacaatgac agaacatttt
    25021 ggacacatta tggtcttaaa tagtttgaca aaaagagatt atttcaagga tccaggactt
    25081 gaaaagactt taactggtga tggttttgcg caaatcattg ccggcttctt aggggcacct
    25141 ccggtcactt cttatggtga aaacatcggt gtaatggctt tgaataaaat attctcggtc
    25201 tatgttattg caggtgccgc agtgattgcc gctctcctca gttttatcgg taaggtatca
    25261 gccttaattc aatctattcc aacaccagtt attggaggta tatcagttgc cttgtttggt
    25321 gtgatagctt ctagcggttt gaaaatcttg attgaatcca aagttgatat ggataataag
    25381 aaaaacttat tgattgctag tgttattttg gtctctggga ttggaggatt gatgcttcaa
    25441 gtaaatggtc ttcaaatttc aggtgtcgct ttctcaacac tccttggcat catcttatat
    25501 caggtacttc ctgaaaaata ggaagaattg ttgttagacc acttacatag tcatcagtcg
    25561 ttagtgataa aggggataat catgtcagtt gtaaataatc gcgttgcact taccaattta
    25621 gtttcaatgg aagcattaac caccgaagaa gttttaggtc ttatcaaccg tggttcagaa
    25681 tataaggctg gtaaagttgt tatttcggat catcaaaagg atcttgttgc caatttattc
    25741 tttgaaaact ccacccgaac ccataagtct tttgaagtgg ctgagaagaa actcggcttg
    25801 acagttcttg atttcaatgc tgatgcaagt gcggttaaca agggagaaag tttgtatgat
    25861 actgtgttaa ccatgagtgc acttggaaca gacatctgtg tgattcgtca cccagaagat
    25921 gattattaca aggaattagt agaaagtcca actattacag cttccattgt caatggagga
    25981 gatggttctg gacaacaccc gagtcaatgt ttacttgact tattaaccat ctatgaagaa
    26041 tttggacgct ttgaaggctt aaagattgcg atcgctggtg acttaactca ctctagggtg
    26101 gcaaagtcca acatgcaaat cctcaaaaga ttaggagcag agctttattt ctatggacct
    26161 gaagaatggt attcagaagc ctttaatgct tacggcactt atattgctat tgaccagatt
    26221 attaaggaat tggatgttct tatgttgctg cgtgtacagc acgaacgtca tgacggccat
    26281 cagagttttt caaaagaagg ttaccatcaa gcgtttggat tgacacagga acgttaccaa
    26341 caattaaaag attctgctat tattatgcat ccagctccag ttaatcggga tgtggaaatt
    26401 gcagatagtt tggtggaagc tcctaaagcg agaattgtat ctcagatggc taatggtgtc
    26461 tttgttcgca tggctattat tgaagccatt ttgaatggta gaaataaaaa ctcatgatga
    26521 aagattaaag catgtttaga aggtaggaaa aggtaggaaa atgacaaaac gattacttat
    26581 tttagaagac ggcactattt ttgaaggaga accctttggt gctgatattg atgtgactgg
    26641 tgaaattgtc tttaatacag gaatgacagg ctaccaagaa tccatcactg accaatccta
    26701 caatgggcaa attttaacct ttacctatcc tttgattgga aattatggga ttaatcgaga
    26761 tgactatgag tccatttcac ccacctgtaa aggggttgtt gtgtctgaag tcagtcggtt
    26821 ggcaagtaat tggcgcaagc aaatgacgct agatgcgttc ttgaaaataa aagggattcc
    26881 agggatttcg ggaattgata cgagagcttt gaccaaaatt attcggcaac acggtaccat
    26941 gaaagctacg atggcagatg atggagatag tatccagcat ttaaaagatc aactgcgtgc
    27001 aacagtctta ccgactaaca ccattgaaca agtgtcaact aaaacagctt atcctgcacc
    27061 agggatcggg aaaaatattg tattggttga ttttggttta aaacattcta ttttaagaga
    27121 attttctaaa agacaatgta atatcaccgt tgtccctttc aacatcactg cagaagaagt
    27181 gctccaacta aatccggatg gcttgatgct atctaatggc ccaggaaacc cagaagattt
    27241 gccagaggct ttagacatga ttcgtggggt acaaggtaag attcctattt ttggtatttg
    27301 tatgggacat caactcttta gtctagcaaa tggtgctaag acttgtaaaa tgacctttgg
    27361 tcatcgtggc tttaaccatg ccgttagaga aattgcaacg ggaagaattg attttaccag
    27421 tcaaaaccat ggttatgccg ttgaacgtag tagtctacct gatactttaa tggttacgca
    27481 tgaagacatt aacgataaga cggtcgaagg agtgaaacat agagattttc cagctttttc
    27541 agtccaattt caccctgatg cggctccagg acctcatgat gccagctact tatttgacga
    27601 atttttggaa atgattgatt cttggagatg tacgagtaaa tagtaaatca gttgcaattt
    27661 cacgactata tcacctcttg ttactctatt tattaacatt tacagtaata ttatttaatg
    27721 ttgctctgtg aggcaacaaa tagagaaagg aaggcaagga gaacacaagt aaataattta
    27781 gtaaaaagtt aggttttcaa agagataaag ctcttattga gaagtgctga ctttaccgct
    27841 tcttgtggta ttcttggtat cattaattat gccaaaacga aaagatattc aaaaaatcat
    27901 ggttatcggg tctggcccga ttattattgg acaagctgct gaatttgatt atgcagggac
    27961 tcaggcctgt ttggctctaa aagaagaagg ctacaaagtc atacttgtta attcaaatcc
    28021 tgcaacgatt atgacagata aagaaatcgc tgataaggta tatattgagc ctttgactct
    28081 tgaatttgtt aatcgtatca ttcgaaaaga aaggccagat gccatactgc caaccttagg
    28141 tggccaaacg ggcctcaata tggcaatggc gttatctaaa gctggtattc ttgatgattt
    28201 agagattgaa ttgttaggta ctaagttatc tgcgattgat caggcagaag acagagacct
    28261 tttcaaacaa ttaatgcaag aactagacca acccattcca gaatctacaa ttgttaaaac
    28321 agttgatgaa gctgttacat ttgctagaga tatcggttat ccagtcattg tgcgcccagc
    28381 ttttactctt ggtggaactg gtggaggaat atgtagtagt gaagaagaac tttgtgaaat
    28441 tactgaaaac ggcctaaagt tatcaccagt cacacagtgt ttgattgaac gctcaattgc
    28501 tggcttcaaa gaaattgaat acgaagtaat gcgagatagt gctgataacg cgctggttgt
    28561 ttgtaatatg gaaaactttg atcccgtagg gattcacaca ggagattcta ttgtatttgc
    28621 accaacacag accctttctg atattgaaaa tcaaatgtta cgtgatgcta gtttaaaaat
    28681 aattcgagct ctgaaaattg aaggtggctg taacgtccag ttagcactag atccttatag
    28741 tttcaaatat tatgttattg aagtaaatcc tagagtgtct cgttcctctg cccttgcttc
    28801 aaaagcaaca ggttatccca ttgccaaatt ggctgcaaag attgctgttg gcctaacact
    28861 tgatgaaatg attaacccta tcacaggcac tacttacgct atgtttgaac ctgcccttga
    28921 ttacgttgtt gctaaaattc cgcgttttcc gtttgataaa tttgaacatg gtgaacgcca
    28981 actgggaact caaatgaaag caacaggtga agtcatggct attggccgta atttggaaga
    29041 gtccttacta aaagcctgtc gttctttaga aattggtgtt tgccataatg agatgacaag
    29101 tttaagtaac attagtgatg aagagttagt gactaaagtg attaaagctc aagatgatcg
    29161 acttttttac ttgtcagaag caatacgacg aggttatagt atcgaagaac ttgagagctt
    29221 gaccaagatt gatttatttt tcttggataa attacttcat attgttgaaa tagagcaaga
    29281 attacaaatg catgttgacc atttggaaag cctcaaaaaa gcaaagcgtt atggattttc
    29341 agatcaaaaa attgcagaaa tttggcaaaa ggatgaaagt gatatcagag cgatgagaca
    29401 ttctcatagc ctgtatcctg tctataaaat ggtagatact tgtgcagctg aatttgatgc
    29461 taaaacccct tatttttatt caacatatga gttggaaaat gaatctgttc agtcgaataa
    29521 agaatcaatt ttagttttag gttcaggccc tattcgtatt ggtcaagggg ttgaatttga
    29581 ctacgcaacg gttcattctg ttaaagccat ccaaaaagct ggctatgaag ccattatcat
    29641 gaattcaaat ccggagactg tatctacaga tttttcggta tcagataaat tatattttga
    29701 accgttaacc tttgaagacg tcatgaatgt gatagattta gagcagccta agggagtaat
    29761 agttcagttt ggaggacaaa ctgctattaa cttagcacaa gcactttctg aggcgggtgt
    29821 tacaatatta ggaacacaag tagaggattt ggatagggct gaagacagag atttatttga
    29881 aaaggcgttg aaagagcttg gaatcccaca accacaaggt caaacggcaa ctaatgaaga
    29941 agaagcttta gaggcagcta aaaaaattgg ttttccagtc ttagttagac cttcttacgt
    30001 acttggtggc cgggctatgg aaattgttga aaacaaagaa gatcttagag aatatataag
    30061 aacagcggtt aaagcaagtc ctgaacatcc tatcttagtg gactcttata ttttcggtaa
    30121 ggagtgtgaa gtggatgcca tttcagatgg aaaatcagtc ttaattcctg gtattatgga
    30181 gcacatcgaa agagcaggtg ttcattcagg agattcaatg gcagtttatc cgccacagca
    30241 attatctaag caaattcaag aaacaatagc tgagtatact aaacgtttag ccattgggtt
    30301 aaattgtatt ggtatgatga atgttcaatt tgttattaaa aatgaacaag tttatgttat
    30361 tgaagtgaat ccacgcgcta gtcgtaccgt acctttctta tcaaaagtaa caggtattcc
    30421 gatggctcag attgctacta agttgatttt aggtcaaaca ttaaaggatc taggatatga
    30481 agatggtctt tatccccaaa gtccactggt gcatatcaaa gcgcctgtgt tttcatttac
    30541 aaagttggca caggttgata gtcttttagg tcctgaaatg aaatcaactg gagaagtaat
    30601 gggttcggat actagtcttg aaaaagcact ttataaagcg tttgaagcca ataattctca
    30661 tttatcagaa tttggtcaaa ttgtctttac tatagcagat gatagtaagg cagaagctct
    30721 aagtctagca agacgattta aggcgattgg ctatcaaatt atggctactc aaggtacagc
    30781 agcttacttt gctgagcaag gcttaagcgc gtgtttagtt ggaaaaattg gtgatgctgc
    30841 caatgacatc ccgacactcg tgcgacatgg tcacgttcag gcaattgtta atacagttgg
    30901 aattaaacgc acagctgata aagacggaca gatgattaga agttcagcta ttgaacaagg
    30961 agttccttta tttacagctt tagataccgc aaaagctatg ttaacggttt tagaaagcag
    31021 atgctttaac atcgaagcga tataaaaatt aaaaaaatca tttttaagaa aacttaaaaa
    31081 tgattttttt agattataat gacaaaagtt tcagtaagct attaatttgt catagcatat
    31141 aagtcattat cttgtggctt gttttcgact aagggctaag aatttcttga caacaatata
    31201 aaaaggtcat atacttgtct tatacaaata gtacaaaaga ggaggataga aatgtctaaa
    31261 agaggaaaaa ttaaaataac aacgaaaaca aagcttatta cagctagtgt tataacgcta
    31321 gtattaatta taactggaat agtcttgtgg aaacaacaac gaaatacgct aacagctgat
    31381 atcgctaaag aaccttactc tactgttagt gtaactgaag ggagtattgc ttcttcgact
    31441 ttactatcag gtactgtaaa ggctttatca gaggaatata tttattttga tgctaataaa
    31501 ggaaatgatg caactgttac agttaaagta ggtgatcagg taacgcaggg ccagcaatta
    31561 gttcaatata atacaacaac agctcagtca gcttatgata ctgctgttag gagtcttaac
    31621 aagattggcc gacaaattaa tcatcttaaa acatacggag ttcctgctgt tagtacagaa
    31681 actaatagag atgaagctac cggtgaagag acgacaacaa cagttcaacc atcagctcag
    31741 caaaatgcta attataaaca gcagctgcaa gatttaaatg atgcttatgc agatgcacaa
    31801 gcagaagtaa ataaagcgca gatagcgtta aatgatacag tagttatcag tagtgtctct
    31861 ggaactgttg tggaagtaaa taatgatatt gatccttctt caaagaacag tcaaacactt
    31921 gttcacgtag caaccgaagg acagcttcag gtgaaaggaa cattgacaga gtatgattta
    31981 gcaaacgtta aggttggtca atctgtaaaa attaagtcta aagtttattc taatcaagaa
    32041 tggactggaa aaatatcata tgtttcaaac tatccaactg agtctaatgc aggttcaaca
    32101 acgccagcag gtagcactgg agcgggaagt tctacaggag ctacctatga ttacaagatt
    32161 gatattataa gtcctctcaa ccagcttaaa caaggcttca ctgtttctgt tgaggttgtt
    32221 aatgaagcaa aacaggcctt agttccttta acggctgtta ttaagaaaga taaaaaacac
    32281 tatgtttgga cttatgatga tgctactggc aaagccaaaa aagtagaggt gacacttgga
    32341 aacgcagatg cacaacaaca agaaattcat aaaggagtag ctgttggtga cattgttatt
    32401 gccaatccag ataaaaatat caaaccggat aaaaaactag aaggggttat ttcaataggt
    32461 accaacacaa aaccggaaaa agattctcaa tcaaagaata aaaaatcagg ggtggataaa
    32521 tagtgtcgca tgaaaaaaaa caattaatgc aattatcaaa tattgtgaag agctatcaaa
    32581 atggggatca agttcttaaa gttttgaaag gtattaattt aacagtatat gagggtgaat
    32641 ttttagctat tatgggtccg tctggttcgg gaaaatcaac actaatgaat attattggtt
    32701 tattagatcg tccaacatcc ggagattaca cattacataa tactaaaata gaaatcctta
    32761 atgatcgtga attagcaaaa gtacgaaatg atgaaatcgg ctttgtcttt caacaattct
    32821 ttctgttagc taaattaact gcattacaaa atgttgaatt gcctcttatt tatgctggtg
    32881 taaacgtctc aaaaagacgt gaacaggcta agcaatttct tgaaaaagta ggacttggac
    32941 gacgtatcaa acatctaccg tcagaactgt caggaggtca aaagcaaagg gtggctatcg
    33001 cacgagcttt agtaaatgac ccatctatta ttttagcgga tgaaccaaca ggtgcactag
    33061 atactaaaac aggtcagcaa attatggaac tcttgacaga gttaaacaaa gaaggtaaaa
    33121 caattattat ggttactcat gaaccagaaa ttgctgattt tgcaactcgg aaaattatta
    33181 ttcgtgatgg tgatatcaca acggatacta cagctagtgt tgttattgac taggaggaaa
    33241 aataatggaa aattggaaat tcgctcttag ctcaatttgg gggcataaga tgcgctcaat
    33301 cctgacaatg ttgggaatta tcatcggtgt agcagcagtt gtgattatta tggggttggg
    33361 aaatgctatg aaaaatagtg taacaagcac attttctagc aagcaaaaag atattcaact
    33421 ctattttcaa gaaaaaggag aagaagaaga cctgtatgcc ggattgcaca cgcatgaaaa
    33481 taatcacgaa gtgaagccag agtggttaga acaaattgtt aaagatatcg atggtattga
    33541 ttcttattac tttaccaatt ctgctacttc tactatttcg tatgaaaaaa agaaagtaga
    33601 caatgcttcg atcatagggg taagtaaaga ttattttaat ataaaaaact atgatatcgt
    33661 agctggtcgt acactaacag ataatgatta ttctaatttt tcgcgtatca ttcttttaga
    33721 tacagtatta gccgatgacc tatttggcaa aggtaattat aagtcagctt taaataaagt
    33781 ggtaagttta tctgataaag attatttagt tattggtgtt tataaaactg atcagacgcc
    33841 agtttctttt gatggattat caggcggcgc tgtcatggcg aatactcagg tagcttcaga
    33901 gtttggcacc aaagaaatag gatctatcta cattcatgtg aacgatattc aaaacagtat
    33961 gaacttagga aatcaagcag ctgatatgct aaccaacata tcgcatatta aagatggaca
    34021 gtacgctgtc cctgataata gcaaaatcgt tgaagaaatc aatagtcagt tcagtatcat
    34081 gacaacagtc ataggttcaa ttgcagcgat ctctctttta gtgggaggaa ttggtgttat
    34141 gaatattatg ttggtatctg tcacagagcg aactagagaa attggtttac gtaaagcact
    34201 tggtgcaaca cgcctgaaaa ttttatcaca atttttgatt gaatccgttg ttctaacagt
    34261 tcttggaggc ttgataggat tattattagc tcaattaagt gtaggggctt taggaaatgc
    34321 gatgacatta aaaggagctt gtatttctct tgatgtggcc ttaatagcag ttttattttc
    34381 tgcctcaata ggtgtctttt ttggaatgtt gccagctaat aaagctagta agttagatcc
    34441 aattgaggca ttacgttatg aatagaatag ttcgtatcaa ttaatctata gacgctgact
    34501 agtttaacgt ctgattatac ctagtttttg tattagcaca gtagttatat cattgaagtg
    34561 ttagtgcatt ttttgaggtg ataatatcta gcaaagctat cattggtatg atgatgtgga
    34621 tagcgtataa gtgatacatc tcttatttct agctgaactt cggttcggct aggaagttgt
    34681 caggcaatga gttgtttaaa ataaaggaga ttagttcaat gacattttta tctgatttga
    34741 tatcattaat gacaaaaatc agattatctt gggtaataaa ggcgggtatt tttcaattat
    34801 tatttgtaac gattgctaat attgtcttat cagaattttt ctattttata ttagacgtta
    34861 ctggtcaata tcatttagat aaagacaatg ttgtgacttt tttaaaaaat cctatagcac
    34921 ttgctttatt aggtgcctat ttatttttat tagctgcttt tattcacctt gagttttttg
    34981 ctctatatcg aattattgcg gatcaagaaa ttagtttcta tctttttaga aaacagtttt
    35041 cttattacct aagggggctt tggaaaacat tttctggtta ccaattatta ctttttttgc
    35101 tttatatcct attgactatt ccagtcttac atattggttt atcttctgtg attactcaaa
    35161 agctttatct tccagaattt attgttgggg aattatcaaa gataactagc acaaagtact
    35221 tgctttatgg cagtcttatt cttgtgtttt accttaacct aagattagta tattttttac
    35281 cattgatagc aatcaaccat cgtacggttg ctcaagcatg gagagagagt tggcaaaaga
    35341 ctaaaaagaa acatgtattg ttatggatga aactttttgc aatcaatggt cttacgattg
    35401 tagtcttatc gctagctatt tccatgattc ttatttttgt tgatatgttt aatcctaagg
    35461 ggaataatat tattgttcag ctgggagctt tgacctttac atgggaactc atttttttta
    35521 ctactatttt ttttaaactc tgttcagcaa tgattttaaa agaggcaatt gaaccacaaa
    35581 agcaatatga tgagccaaga agaagtaata aggcatatgt tgtaatcttt atcgtggtta
    35641 cagtaggttt tgcttatcaa tctcttgaac gtttaacttt ttttgacaca tctcactcta
    35701 agacagttat cgcgcataga ggacttgtat cagcaggtgt agaaaattct ctggaagccc
    35761 ttgaaggtgc taagaaagca ggaagtgatt atgtagaact ggatctaatc ttgactaagg
    35821 ataatcactt tgtggtgtct catgataatc gattgaagcg tttagctgga gtaaataaga
    35881 cgattcgcaa cttaacctta aaagaagttg aacatctaac gagtcatcaa ggacattttt
    35941 cagggcgttt tgtttctttt gacacctttt atcaaaaggc taagaagttg aatatgccat
    36001 tacttattga actcaagcca attggtacag aacctggaaa ttatgtcgat ttgtttttag
    36061 aaacttatca tcgacttggt ataagcaaag ataataaagt catgtcttta gatttagaag
    36121 taatagaagc tatcaagaaa aaaaatccat caattacgac tggttatatc ataccaattc
    36181 aatttggatt ttttggagat gaatttgttg atttctatgt cattgaagac ttttcttatc
    36241 ggtcttattt gtcgtcccaa gctttttgga ataataaaga aatttacgtt tggactatta
    36301 atgatcccaa gcgcatagag cattatctcc taaagcctat tcagggaatt attacagacc
    36361 aaccagcttt aactaatcaa ttgattaaag acttaaaaca agataattct tattttagtc
    36421 gattagtcag aattattagt agtctttatt aaaggtttaa ggaaagtgtc aagaaaaaaa
    36481 cttgacactt tctttaaact gagttatact gtttaacata ggaatttagc gtagctgtat
    36541 ttcttataca ttactaaaag aaaagagact atttaaaaat ggcagtaaaa atccgtttaa
    36601 ctcgtatggg ttctaagaaa aaacctttct accgtattaa tgttgcagat tcacgtgcac
    36661 cacgtgatgg tcgttttatc gaaacagttg gaacatacaa cccattagtg gctgaaaatc
    36721 aaattactat taaagaggat cgtgttcttg agtggttatc taaaggagcg cagccttctg
    36781 ataccgttcg taacatcttg tctaaagctg gtgttatggc taagttccac gaccaaaaat
    36841 tctctaaata ataacagcct atggatacca ttgaaaatct tattatcgct attgtgaaac
    36901 ctttgatttc acaaccagat aatcttacca ttaaaattga agatactcct gactttttag
    36961 agtatcatct tgacttggat gctcaagaca tcggtcgtgt tatcggcaaa aaaggtcgaa
    37021 ctattactgc gataagatcg attgtctact cggttccaac actaggaaaa aaagttcgct
    37081 tagttattga tgagaaataa tcactaactt gagatggatt gaaaagcttg ttgacttaaa
    37141 gtcaacaagc tttttgaata gcaaaaatct taatatttct gaaatgaaaa gattagattt
    37201 ttctgtcttt aactaaaaag aagttaaaga atggcttagc tgattgcctt tataagagat
    37261 tatactggtt tttgatagct gctgtcatgt aaaatagcta caattcatct tacgagatag
    37321 tctttatttt ccaacctgct gtttgttgta tgtttatagt ttcctaaaaa atgctctctc
    37381 agttttttgg ggggcattcc taaaattaag tatacttaat tttaggaatg tttatattct
    37441 ttacattctg atgaaaatac aaattatcct aacatgatgt attgaaaggg gaaatagttt
    37501 atgttagtat actgattaag tgaagctgta atgaggctta tacgatcaaa aaagttagag
    37561 taactttata aaacagtggt gttgaagcaa gtttagcttc ttctcagaaa tagattatct
    37621 ataaaaaatc ccaaaagtag tttgtttcca gttaatgaac ttcttttggg aaggcttttt
    37681 tgctgatcgc cacttcactt gtatatcggg aacattcacg ctaagatgta taagttgaat
    37741 cgtttgtcta tggcttgatg aaaattaatc aaaagataat gttcaactct ataattagta
    37801 caagcaaaat gagatagctt attcattagt aagcataaga tataacaaga gaaattacct
    37861 taaaacagga agtactagag tatttaaaat aagtgataga cttggatatt attaggtcga
    37921 ctagtgttct tttcaataat ttaatatcgt tattattagt atcagccaat caaatgtgga
    37981 taagttggct tttagaaagg ttttaccaca atgaagaaac atcttaaaac agttgccttg
    38041 accctcacta cagtatcggt agtcacccac aatcaggaag tttttagttt agtcaaagag
    38101 ccaattctta aacaaactca agcttcttca tcgatttctg gcgctgacta cgcagaaagt
    38161 agcggtaaaa gcaagttaaa gattaatgaa acttctggcc ctgttgatga tacagtcact
    38221 gacttatttt cggataaacg tactactcct gaaaaaataa aagataatct tgctaaaggt
    38281 ccgagagaac aagagttaaa ggcagtaaca gagaatacag aatcagaaaa gcagatcact
    38341 tctggatctc aactagaaca atcaaaagag tctctttctt taaataaaac agtgccatca
    38401 acgtctaatt gggagatttg tgattttatt actaagggga atacccttgt tggtctttca
    38461 aaatcaggtg ttgaaaagtt atctcaaact gatcatctcg tattgcctag tcaagcagca
    38521 gatggaactc aattgataca agtagctagt tttgctttta ctccagataa aaagacggca
    38581 attgcagaat ataccagtag ggctggagaa aatggggaaa taagccaact agatgtggat
    38641 ggaaaagaaa ttattaacga aggtgaggtt tttaattctt atctactaaa gaaggtaaca
    38701 atcccaactg gttataaaca tattggtcaa gatgcttttg tggacaataa gaatattgct
    38761 gaggttaatc ttcctgaaag cctcgagact atttctgact atgcttttgc tcacctagct
    38821 ttgaaacaga tcgatttgcc agataattta aaagcgattg gagaattagc tttttttgat
    38881 aatcaaatta caggtaaact ttctttgcca cgtcagttaa tgcgattagc agaacgtgct
    38941 tttaaatcaa accatatcaa aacaattgag tttagaggaa atagtctaaa agtgataggg
    39001 gaagctagtt ttcaagataa tgatctgagt caactaatgc tacctgacgg tcttgaaaaa
    39061 atagaatcag aagcttttac aggaaatcca ggagatgatc actacaataa ccgtgttgtt
    39121 ttgtggacaa aatctggaaa aaatccttct ggtcttgcta ctgaaaatac ctatgttaat
    39181 cctgataagt cactatggca ggaaagtcct gagattgatt atactaaatg gttagaggaa
    39241 gattttacct atcaaaaaaa tagtgttaca ggtttttcaa ataaaggctt acaaaaagta
    39301 aaacgtaata aaaacttaga aattccaaaa cagcacaatg gtgttactat tactgaaatt
    39361 ggtgataatg cttttcgcaa tgttgatttt caaaataaaa ctttacgtaa atatgatttg
    39421 gaagaagtaa agcttccctc aactattcgg aaaataggtg cttttgcttt tcaatctaat
    39481 aacttgaaat cttttgaagc aagtgacgat ttagaagaga ttaaagaggg agcctttatg
    39541 aataatcgta ttgaaacctt ggaattaaaa gataaattag ttactattgg tgatgcggct
    39601 ttccatatta atcatattta tgccattgtt cttccagaat ctgtacaaga aatagggcgt
    39661 tcagcatttc ggcaaaatgg tgcaaataat cttattttta tgggaagtaa ggttaagacc
    39721 ttaggtgaga tggcattttt atcaaataga cttgaacatc tggatctttc tgagcaaaaa
    39781 cagttaacag agattcctgt tcaagccttt tcagacaatg ccttgaaaga agtattatta
    39841 ccagcatcac tgaaaacgat tcgagaagaa gccttcaaaa agaatcattt aaaacaactg
    39901 gaagtggcat ctgccttgtc ccatattgct tttaatgctt tagatgataa tgatggtgat
    39961 gaacaatttg ataataaagt ggttgttaaa acgcatcata attcctacgc actagcagat
    40021 ggtgagcatt ttatcgttga tccagataag ttatcttcta caatagtaga ccttgaaaag
    40081 attttaaaac taatcgaagg tttagattat tctacattac gtcagactac tcaaactcag
    40141 tttagagaca tgactactgc aggtaaagcg ttgttgtcaa aatctaacct ccgacaagga
    40201 gaaaaacaaa aattccttca agaagcacaa tttttccttg gccgcgttga tttggataaa
    40261 gccatagcta aagctgagaa ggctttagtg accaagaagg caacaaagaa tggtcagttg
    40321 cttgaaagaa gtattaacaa agcggtatta gcttataata atagcgctat taaaaaagct
    40381 aatgttaagc gcttggaaaa agagttagac ttgctaacag gattagttga gggaaaagga
    40441 ccattagcgc aagctacaat ggtacaagga gtttatttat taaagacgcc tttgccattg
    40501 ccagaatatt atatcggatt gaacgtttat tttgacaagt ctggaaaatt gatttatgca
    40561 cttgatatga gtgatactat tggcgaggga caaaaagacg cttatggtaa tcctatatta
    40621 aatgttgacg aggataatga aggttatcat gccttggcag ttgccacttt agctgattat
    40681 gaggggctcg acatcaaaac aattttaaat agtaagctta gtcaattaac atctattcgt
    40741 caggtaccga ctgcagccta tcatagagcc ggtattttcc aagctatcca aaatgcagcg
    40801 gcagaagcag agcagttatt gcctaaacca ggtacgcact ctgagaagtc aagctcaagt
    40861 gaatctgcta actctaaaga tagaggattg caatcaaacc caaaaacgaa tagaggacga
    40921 cactctgcaa tattgcctag gacagggtca aaaggcagct ttgtctatgg aatcttaggt
    40981 tacactagcg ttgctttact gtcactaata actgctataa aaaagaaaaa atattaaatt
    41041 gatttaagga gaaaaaatga taacacaaga aatgaaagat cttattaata accaattagc
    41101 aatggttgcg acggttgatg ccaaaggtca acccaatatt ggtccaaaac gttctatgcg
    41161 tctctgggat gataaaacat ttatttataa tgaaaataca gatggtcaaa ctcgtattaa
    41221 cattgaagat aatgggaaaa ttgaaattgc ctttgttgat cgtgagcgcc ttttaggtta
    41281 caggtttgta ggaactgctg aaattcaaac agaaggtgct tattacgaag cagcgaaaaa
    41341 atgggcacaa ggacgcatgg gggtaccaaa agcagttggt attattcatg tcgagcgtat
    41401 ttttaattta caatctggtg ctaatgcagg taaagaaata aacagtgaaa gtaactaaaa
    41461 ttaactgtaa cagtatttag gacccttatt ttaaacgcta aaaaacaagc atactctttt
    41521 ttacagcata gtgactatgt tgtaaaaagg ggtatgctta ttaggtgctc ttaatattta
    41581 tagataactg cacctaataa gttttcaata gacttatagc acattaattg acttcaatta
    41641 gttgacttca atactagaaa gagaagaaca gtgatgttgg tgttcgttta aagaagtatc
    41701 gatttccaca gtgactgagt ttatattgta cctttgacaa atcagtctaa tagatttttt
    41761 gcaatgtttt tcagtggtag attctctcaa gcagcaatgt atagttgctc tatgatcaat
    41821 accatccatc gaccaaacat taagctgatt aacagacact atatgaggaa gttggctgag
    41881 ttcatggtgc aaagtgcaat agtctataga atcaggaaca ccatctaaaa aaatgttagc
    41941 agttgctact aactttggaa gagctttaga taatataaaa gaagcaatgg caattgataa
    42001 taaaggatca agaatgtacc aaggtttcca ttttaagatc agtgacagta tgataatggc
    42061 taaccaccca aggatatctt ctaaaaagtg aaggcttaat atttcttcat tttttgtctg
    42121 gttagagtga atgatgaagc tagcaaatcc attgattata attgcaaaaa tagctaaaac
    42181 gaacataccg tcataattaa caatagtagg atgccataat tttggtatcg tttcaatcat
    42241 tactaataat gacccgctaa taagtattag atttgtcgtt agcgctccta aaaggctaaa
    42301 tcgcttatag cctagtgaaa aatttggact ctctttttta acagcctttc tttctaagat
    42361 agctgagata ccaatagcaa tggcatctcc aaaatcgtgg acagcatctg ctaaaatagc
    42421 acccgaaaag aataatgtcc caaaaataaa ttcaatgagg gaaaaactaa gatttaatat
    42481 aaaaataatg gtgacttttt tacttgctgg cataattttc tccttttgtt atactggtaa
    42541 tatgtatagt agaatgcttt attctattat ctaaaagtta aaatacaatt actataacca
    42601 aaaatgagca cctgttcaat agtgaacaac atttagaaag tgtccgatat gacaaaagac
    42661 agacaaatca aaaaaacaaa aacagctatc tatagtgctt ttattgcttt attacaaaaa
    42721 aaagaatatt cgaaaataac tgttcgagat atgattacac ttgctaatgt agggagatca
    42781 accttttatg cacattatga aagcaaagaa atgttactaa aagaactctg tgaagaactt
    42841 tttcatcatt tgtttaggca aaaaagaaat gtgacttttg aggactattt ggtacatatt
    42901 ttaaagcatt ttgaacaaaa taaagacagc attgcgacgt tgttactttc taatgatccc
    42961 tattttttat taagatttaa aaacgaacta gaacatgatg tttaccctaa cttgcgctgc
    43021 aagtatatcg acaaaacgac tattccagaa gtattcctaa aacaatttgt tctatctagt
    43081 tttatagaaa ccttaaaatg gtggctccat caaaggcaaa ggatgtctgc aaatgaacta
    43141 ttaaagtatt atttagagct cataaaataa tgactagtga gtcatcttgc caaattcaat
    43201 tttttaatac agacggattt taacaattta acagataaag acaaaactgt aagtgcaaaa
    43261 tagactataa cgtgataaaa taataggtaa tttggttctg aaaaagaagt gtctatcaaa
    43321 ctttagaatc aagagaggtc gctaaagtga cgtgagataa agagttaacg atttaggagc
    43381 taccatggaa tattttaatg ttggaaaaat tgtcaacacg caaggcctgc aaggagagat
    43441 gcgcgtgttg tctgtcagtg attttgctga ggaacgcttt aaaaaaggct cacaattagc
    43501 tttatttgat gataaagatc ggtttgttca agaagtgaca attgttagtc accgtaaaca
    43561 aaagcatttt gatattatca aatttaaaga catgtatcat attaatgcta ttgaaaagta
    43621 caagggatac actttaaagg tctcaaaaga caatcaaggg gatttacaag aaggagaatt
    43681 ttattaccat cagattattg gcatggctgt ttatgaaaag gatgtcctta ttggacatgt
    43741 taaggaaatc ttgcagccag gtgctaatga tgtttggata gtgaaacgcc aaggaaaacg
    43801 agacttgctc ttaccatata ttcccccagt agttcttaac gtagatgttc ctaacaagcg
    43861 cgtggatgtt gaattgatgg aaggcttaga cgatgaagat tgatatttta accctttttc
    43921 ctgaaatgtt tgcccctcta gagcattcca ttgtaggcaa ggcaaaagaa aaaggtctgt
    43981 tagacattca ctaccataac tttcgagatt atgctgaaaa aactcgtcat gtggatgacg
    44041 aaccctatgg tgggggccaa ggcatgttat tgagagcgca gcccattttt gacaccattg
    44101 aacagattga agccaaaaaa ccaaggatta ttcttttaga tccagcaggt aaacccttta
    44161 ctcaggctta tgctgaagaa ttagctcttg aggaagagtt aatttttatt tgtggtcatt
    44221 atgaaggtta tgatgagcgg attaagacct tggtgacgga tgaaatttca ttaggcgact
    44281 ttgtattaac aggaggtgag ttggcggcca tgactatggt ggatgcgacc gtcaggttaa
    44341 ttccacaggt acttgggaaa gaaagtagcc atcaggacga ttctttttca tcaggacttt
    44401 tagaataccc tcaatacaca agaccttatg attatcgtgg tatgacagtt ccagatgttc
    44461 taatgagtgg tcatcacgaa cgtattcgct tgtggcgtct agaagaaagt ctcaaaaaaa
    44521 cttatttaag gcggccagat ttacttgaac attacaattt ttccgaggaa gagcgcaagc
    44581 tattggataa gattaaagaa gcattggatc aaggagaaga ttagtgaaag ataaagcata
    44641 tgatattacg attattggtg gtggtccaat aggtttattt gcagcctttt atgcaggtct
    44701 taggggcgta acagtaaaga ttattgagag tttgtcagaa cttggtggac agcctgccat
    44761 tctttatcct gagaaaatga tttatgatat tccagcttat ccatctttaa caggtgttga
    44821 gttgacagaa aatttaataa aacaattgag ccgttttgaa gatcgtacaa ccatttgttt
    44881 aaaagaagaa gtattaacct ttgacaaggt taaaggtggt ttttcgatta ggactaataa
    44941 ggcagagcat ttttcaaaag ccattatcat tgcttgtgga aatggcgctt ttgcaccaag
    45001 aactttaggg ttagaaagtg aggaaaactt tgctgatcat aacctttttt ataatgttca
    45061 ccagttggac cagtttgcgg gacaaaaagt tgtgatttgt ggtggtggag attcagcagt
    45121 tgattgggct ttggctttag aagatattgc tgagagtgtg acggttgtgc accgtcgtga
    45181 tgctttcaga gcacatgaac acagtgtgga attattgaaa gcatcaacgg tcaacctctt
    45241 aacaccttat gtgccaaaag cattgaaagg aattggaaac ctggctgaaa aattagttat
    45301 tcaaaaagtg aaagaagatg aagtgttaga acttgaattg gacagtctta ttgttagttt
    45361 tggtttttca acttctaata agaatttgaa aaattggaat ctcgactata agcgttcaag
    45421 tattactgtt tcaccacttt ttcaaacaag tcaagaaggt atttttgcca ttggtgatgc
    45481 agccgcttat aatgggaaag tggatttgat tgctactggt tttggcgaag caccaacagc
    45541 agttaatcag gccattaact atatttatcc agatcgagat aaccgtgtgg tgcattcaac
    45601 ctctttaatt gactaaggga gtcggaagtt tcaagagcag tagcaacagc taatattcag
    45661 tttgtcagga gaggacaaat ggtttttacc taagtcttcg tgacaagttt tatactttgt
    45721 gataaaatag tcattgtgaa aaaagtgtga tggttttcac agattaacac attacaatga
    45781 aaaggggtct atcatgacaa caacagataa agagacattt agttctttta tgaataaggt
    45841 cttggctggt acagcgattg ctatcgttgt ggcactcatt ccaaatgcta ttttagcgac
    45901 tttcttaaaa ccgcttttgc caaatatggc agctgctgaa tttttacaca ttgtgcaagt
    45961 cttccaattc ttcacgccga ttatggctgg tttcttgatt gggcaacaat ttaagtttaa
    46021 tcctatgcaa cagttggctg ttggtggagc tgcttatatc ggttctggag cctgggctta
    46081 tacagaagtc attcaaaaag gtgtggcgac aggaacattc caacttagag gtattgggga
    46141 cttaatcaat atgatgatta cagctagcct tgcggtttta gctgtgaaat attttgggaa
    46201 taaatttggc tctttaacca ttatcttgtt acctatcact ataggaactg gcgtaggtta
    46261 tattggttgg aaatttttgc cttatgtgtc ttacgtgaca accctgatcg gacaagggat
    46321 taattctttt acaaccttac aacctatttt aatgtctatt ttaattgcag ttgctttctc
    46381 gcttatcatt gttagtccga tttcaactgt tgcaatcggt ttagctattg ggcttaatgg
    46441 aatggctgcg ggagcagctt ctatgggaat tgcatcgaca gcagcagtct tagtgtgggc
    46501 gacgcttaaa gttaataaat ctggtgtacc aattgctatc gcacttgggg ctatgaaaat
    46561 gatgatgcca aacttcttga aacatcctat catggctatt ccgatggtgt tcactgcagc
    46621 aattagctca ttaacagtac cattgtttaa tcttgttgga acaccagctt catctggttt
    46681 tggcttagtt ggtgcagtag gtccgattgc ttctttagca ggtggtagct caatacttat
    46741 tattatcctt gcttggatca ttgttccgtt tgcggttgct tttgcggcgc ataaggtttc
    46801 taaagatatt ctaaaactgt ataaagaaga cattttcgtc tttgaaggct aataggagga
    46861 aaatatgtta gtttatattg ctggctcagg agccatgggg tgccgttttg gttaccaaat
    46921 ttcaaaaaca aataacgatg tgattttgtt agataactgg gaagatcata tcaatgccat
    46981 taaagaaaac ggtttagtgg tgacagggga tgtggaagaa acagttaagc ttcctattat
    47041 gaaaccgact gaagccactc aagaagccga tttgattatc ttgtttacaa aagcaatgca
    47101 gttgccacaa atgcttcaag acattaaagg cattattgga aaagaaacca aagtgctttg
    47161 ccttttaaat ggtcttggac acgaagacgt tattcgtcag tacattccag agcacaacat
    47221 tttgatgggg gtcactgtct ggactgctgg tctagaaggt cctggtcgtg ctcatcttca
    47281 aggtgtggga gcacttaact tacaaagcat ggatccaagc aatcaagaag ctggacacca
    47341 agtggctgat ttgttaaatg aagctaactt gaatgcgact tatgatgaga atgttgtgcc
    47401 aaacatctgg cgaaaagctt gcgtgaatgg taccatgaac tcaacttgtg cccttctaga
    47461 ttgtacaatt ggtgaattat ttgccagtga agacggtctc aaaatggtaa aagaaatcat
    47521 ccatgagttt gttatcgttg gtcaagcaga aggtgtcgaa ctaaacgaag aagaaatcac
    47581 acaatatgta atggatacat ctgttaaagc tgctcaccat tacccatcaa tgcaccaaga
    47641 ccttgtgcaa aatcaccgat tgactgaaat tgattttatc aatggtgcag tcaataccaa
    47701 aggtgaaaag cttggtatca atactccata ctgccgtatg attacagagc tagttcacgc
    47761 taaagaagca gtgttgaaca tccaataaac gctatcaaat cgttatactc aatattatgg
    47821 ctaaagcttt aatattgagt tttttgttat gctgatagta tttttcttag gcgtcattat
    47881 ttgtgagcct agattatgag ttaataaaag aaataataat tggtagaatt tgatagattt
    47941 tgaaaggtta tgcttgactt ttagttataa aatagtataa taattgtaaa cgatttcagt
    48001 taggggtggt tctatcttaa aatcaaaacg aaaacaatta atcatggcaa agatcactga
    48061 agaaaactat gtttctttag aggatttaat gcaattactc aactcctctg agtctactat
    48121 ccgtagagat ctaggagagt tggagcaaga aggacggcta catcgtgttc atggaggtgc
    48181 tgaacttttt cactctttac aagaggaact ttcgaaccaa gaaaagtctg tcaaaaacag
    48241 tcatattaaa aaagctattg cgcaaagagc ctctcagctt atctatgaca acgatgtgat
    48301 ctttattgat gctgggacaa cgactgaatt tttattacct tttttacaag ctaaaaattt
    48361 aactgtagtt actaattcta ttcatcatgc tgctagatta gttgaactct ctattgagac
    48421 gattattgta gggggttatg tcaaacaaac gaccgatgcc agtattggta acgtagcttt
    48481 ggaacagatt aggcagatga attttgacaa ggcatttctt ggaatgaatg gtgttgatga
    48541 ttcttacctg acgactcctg atatggaaga agcagttatt aaaaaagcgg ttctgtcaaa
    48601 tgcaaaatta gcttacattt tagtagatgg cactaaaatt ggtcaggtat cgtttgtaaa
    48661 agtagctcct attaatgatg tgactattat tacattagga ggatcagcaa gtattcttaa
    48721 acaaataaag gaaaaggcaa aggtgattga attatgattt ataccgtgac cttaaaccct
    48781 tctattgact ttattgtcag aattgaccaa ataaatcttg ggtcagtgaa tcgcatggcg
    48841 agtgatgata agtttgctgg aggtaaaggc attaatgtga gtcgcatctt gcaacgttta
    48901 gacattgcta gcacggcgac agggttcttg ggtggtttta caggacgttt tatcgaagaa
    48961 agtttgagtg cagaaggcgt taaaactgat tttgtaaaag gtgatcaaga tactcgaatc
    49021 aatgttaaaa tcaagtcaca agaagagacc gaattaaatg gtcaaggacc tatcattagt
    49081 caggaacaac tggaggattt gaaaactaag ctttcccagc taacagcaga ggatacagtt
    49141 gtctttgcag gttcagcgcc agctaatctt ggcaatgctg tttataagga attacttcct
    49201 ttagttaggc aaagcggtgc tcaggtggtt tgtgattttg aaggacagac tttaatagat
    49261 gcgcttgcat ataatcctct gttggttaaa cctaataatc atgaactaga agctattttt
    49321 ggtacgattc tgaccagtct tgatgatgtt gaaacctatg cccgtcgtct tttagagatg
    49381 ggtgctcaaa acgtcatcat ttcaatggct ggtgatggtg ctttgctggt tactaaagaa
    49441 gcgacttatt ttgctaaacc gattaaggga gaagttaaaa attctgtcgg tgctggtgat
    49501 tcgatggttg ctggctttac aggcgaattt atgaagagtc aaaatccaat agaagcctta
    49561 aaatggggcg tggcttgtgg tacagcgacg gctttctctg atgacttggc gacaattgca
    49621 tttattaagg aaacatatca taaagttgag gtagaaaaac gatgaaaatt caagatttat
    49681 tgagaaaaga tattatgatt ctcgatttac aggctatttc taaagaagtt gccattgacg
    49741 agatgattac taaattagtt gaaaaagata ttgtacatga ttttgatgtc tttaaaaaga
    49801 gtatcatgac acgtgaagaa caaacatcaa cagggcttgg tgatgggatt gccatgcctc
    49861 attctaaaaa tattgtagta gataaaccag cggtcttgtt tgcgaaatca aataaaggtg
    49921 tggattacaa ggctttagat ggtcaaccaa cggatctctt ctttatgatt gctgcgcctc
    49981 aaggagcaaa tgatactcac ttggctgcac tagcagaatt atcacaatac cttttgaaag
    50041 acggctttgc tgataaactt cgtgctgcag cgacaccaga agcagtcatt gctgtttttg
    50101 atgaagcgtc aacagctaaa gaagaagtgg ttgctccaac aagtggtcaa gactttatcg
    50161 ttgctgttac agcttgtccg acaggaattg ctcacactta tatggcagaa gaggctttga
    50221 aaaaacaagc tgcagaaatg ggagtagcta ttaaagttga aacaaacggt gcttctggtg
    50281 tggctaatcg tttaaccgct gaagatattc agagagctaa aggagttatt gttgcagctg
    50341 ataaagcggt tgaaatggac cgttttgatg gtaaacaatt cattgctcgc cctgttgcag
    50401 atggtatcaa gaaaagtcag gaattgattt ctttgatttt aaacaatgaa ggaaacactt
    50461 atcatgctaa aaatggaaaa tctgaaacag cagtatcaac tgagaaaaca agtttaggcg
    50521 gtgctttcta taaacacttg atgggcggtg tctcacaaat gttgccattt gttattggtg
    50581 gcgggattat gattgctcta gcatttttat tggataacat gcttggtgtg ccaaatgatc
    50641 agcttggaag tcttggatcc tatcatgaaa tagcagctat ttttatgaac attggtggag
    50701 cagccttttc cttcatgtta ccagtactag caggttatat cgcttattct attgctgaaa
    50761 aaccgggctt agtagctggt tttgtggcag gtgccattgc ctcaaatggt cttgcttttg
    50821 gtaaggtacc atttgcagca ggaggagaag tgagcttggg cttaacgggc gtgccatcag
    50881 gtttccttgg ggcgcttgtg ggtggtttcc ttgccggtgg tgttattctt gcccttcgta
    50941 aattgttggc aggcctacca cgttctctag aaggggttaa atctatcctc ctttacccat
    51001 tacttggcgt ccttgtcact ggtttcttga tgctttttgt caatattcca atggcagcta
    51061 ttaacacagc ccttaatgat ttcttacaag gtctttcagg aagctctgct gtccttatgg
    51121 gacttcttgt tggtggaatg atggctgttg acatgggtgg tcctgttaat aaggcagctt
    51181 atgtttttgg tacaggaact ttagcagcta ctgtggcaaa tggtggttcg gtcgttatgg
    51241 ccgctgttat ggcaggtggt atggtacctc ctcttgctgt ttttgtagcc actctcttgt
    51301 ttaaagataa atttaccaaa gaagaacgtg aatcaggctt gacaaatatt gttatgggac
    51361 tttcattcat caccgaagga gcaattccgt ttggtgcagc tgacccagca cgtgcgattc
    51421 ctagctttat cgcaggttct gccttgacag gagctcttgt tggtttggct ggtattaaat
    51481 taatggcgcc acatggtggt atcttcgtaa tcgctttaac aagcaatcca atcttatatc
    51541 ttgtctttgt tgtgattggt gcccttgtat caggtatctt atttggcgct cttcgcaaaa
    51601 aagcctaaaa ggtaaagaac ttttttctta ctaaaactag aatctgtcaa ggttctagtt
    51661 ttttgctata ctagagttaa aggataagaa atgaggacct ttgaaggtga aaaaaaggag
    51721 aagaagggct aaatcatcag taaatcgact tgtcttaggg ttagtattac ttaatctgat
    51781 tgttagtatg tggacactaa aattgggaaa ccagcgatta gctccgtatg cggatcacga
    51841 aactctgaca tttgtcagga agattagtca tgctgctcaa tcagtcgctc aaaagaaaca
    51901 gttatacagt tcggtgatga tggctcaggc cattttagaa tccaataatg gtaagtcaca
    51961 actaagtcaa aaaccttatt ataatttttt cgggattaag gggagctata aagaacggtc
    52021 agtcattttt ccaactttag aagacgatgg acaagggaat ctttatcaaa ttgatgctgc
    52081 ttttcgttcc tatgggagcc tgacagcttg ttttttagat tacgcaagag ttttgaatga
    52141 tccactttat gacaaaaccc acaaaaagtt ttggtctcat tatcaagatg ctactgcaac
    52201 cttaacaggc acttacgcca ctgatacaac ttaccatacc aaattaaatg aattgattga
    52261 atggtatcaa ctcactaatt ttgatggtct aatgaaatag ttatccacaa ttttaaaaaa
    52321 aatcagaaga aatatgtcga tttcaagaca aaagttatca atctcattaa attatctgtg
    52381 gaaaagttaa tttaaacgtt cgtgtgctat aatagaaaca tagaaaatga ataaaaaagg
    52441 atatatgaat aatgacaaaa aagaaaggta agcttgtact tatcagtctt tttgttctag
    52501 cagcttgttt aggagcttat agtgcaatga gacaatctca taaaacttca aacgtgtcag
    52561 ctgagactat cgccagttct tcaacccgac attttattga tgaaattggt ccaactgcta
    52621 gtactattgg tcaagaacgt gatctttatg cgtctgttat gatagcacaa gcgattttag
    52681 aatcaagtaa tggtaaatca agtctaagtc aagcacctta ttataatttt ttcggtatta
    52741 agggtgccta taacggttct tctgtgacga tgtcaacttg ggaagatgat ggtaatggca
    52801 atacctatac gattgatcaa gcctttagag cttatccaag tattgcagac tcccttaatg
    52861 actacgcgga cttgctaagt tctagcactt atataggtgc tagaaaatca aacacgctat
    52921 cgtatcaaga tgctacagca gctctaactg gcttatacgc tacagacacc agttataact
    52981 taaaactaaa taatattatt gcaacctatg gtttgacggc ttatgatgtt gctaatagct
    53041 cagctcaaga aactggtcta gcaacttcgg gttatgtttg gaatgaatat cgtcgtaatt
    53101 acactgatgc ggagacccta gcagttgatg aggcttgggc taaacgcatg acttattaat
    53161 gtaacactaa caacaatctg cttgcactga ccccaaaaaa gctggatact ctattttaag
    53221 taaaggattt agttctgtat tggacaggac taaatccttt tagttttgct ttaatatgtt
    53281 tatcgttgta ataatcaatg tcatttgtaa tcgcttgttc taagtcttct agtgatttat
    53341 agttattctc ataaccatag aacatttctg actttaagat gccaaagaaa gattccatca
    53401 tgccattgtc aggactgttt cttttacgtg acatagacgg acgaattcct ttagagttta
    53461 ggaaatgatg ataatagttg tgttgatatt gccaaccttg atcactctat catatagttc
    53521 cgtcatagca tttatttgga gatgcctttt tccattattg gtttaatgtg atttaagcaa
    53581 ggttattagc taaatttaat gccagacaaa aataccccaa ttattagatt ttttgtctaa
    53641 cttttggggc gcaattcaat ctgagcagtt ttagatttgg tatcaatgtt attataggtc
    53701 aattgatcat atttatccaa agcttttagc ttatttagtc caaagataaa tatcaaaatg
    53761 gctgaaatct ttcatattta tagtcttatt gtctttatat tttttaaaaa tatcactcct
    53821 tgtactagat gaggttgcat catataagtt aaaactttca tgtttattgc cattgatcgc
    53881 aatttctaat tgccctttta tgtatggaga attaggatca taaattttgt atgtttgcat
    53941 aagatattgt ctgattttga agtcaaattc ttgaatcgta aaaataggtt tatctatttt
    54001 tatgggattt attagtgttt tttgctggac tccatctatt agtaaattac ctacaatttt
    54061 actattttcc gaattactgt ttactgatgg tgtaacccca ccatatatat aataaacatc
    54121 ataacgagta aatatgtacg gaagaccaaa aatatttact ttttgatttt tcgtaaactt
    54181 tgttgtaata tagctctcga cctcagaatc aactgaaaaa tttttctttt tataactgga
    54241 aatatcaata taaaaatcat gagtagtcaa gtaatcaata ttacaattcg tataatctac
    54301 tggtatgatt tcgtatgcgt aatttaattg tagcttaacg tctttaatat tttcactatc
    54361 tgattttaca cttcc
//
LOCUS       DAXWIQ010000008        50960 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000008 DAXWIQ010000000
VERSION     DAXWIQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50960
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.008"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            200..937
                     /locus_tag="ACGEUI_000448"
     CDS             200..937
                     /locus_tag="ACGEUI_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFF8165309.1"
                     /translation="MVITSKANQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQV
                     FRHIFVLEEMVERLAGEQELVIVSPQVLKELTDSPSPQGIVAEVEIPKLAFPSDYKGK
                     YLVLEDVQDPGNLGTIIRTADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRT
                     DVYQLCRELQEYETPILATTLSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQ
                     LVHITMPGQAESLNVAVAAGILIFSLI"
     gene            976..1476
                     /locus_tag="ACGEUI_000449"
     CDS             976..1476
                     /locus_tag="ACGEUI_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8165310.1"
                     /translation="MHYTEDKEYMEHVGHLIAHPRFQKLSHIVQHQHSTRLEHSINVS
                     YSSYKLAKRFGWDAKSTARGGLLHDFFYYDWRVTKFNKGHAWVHPRIAVRNAKKLTEL
                     NKKEEDIILKHMWGATIAFPRYKESYIVTMVDKYWAVKEAVTPLRQKWSNRRFLRRKT
                     LQSHNR"
     gene            1491..2180
                     /locus_tag="ACGEUI_000450"
     CDS             1491..2180
                     /locus_tag="ACGEUI_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFF8165311.1"
                     /translation="MNDHVIYTQSDVGLNQFFAKIYSLVGMGVGLSAFVSYLMLYPFR
                     ENLISILVNQPMIYYGAAIIELILVFVASSAARKNTPAALPIFLIYSALNGFTLSFII
                     VAYAQTTVFQAFLSSAAVFFAMSIIGVKTKRDMSGLRKAMFAALIGVVVASLINLFIG
                     SGMMSYVISVISVLIFSGLIASDNQMIKRVYQATNGQVGDGWAVAMALSLYLDFINLF
                     ISLLRIFGRND"
     gene            2358..2600
                     /locus_tag="ACGEUI_000451"
     CDS             2358..2600
                     /locus_tag="ACGEUI_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFF8165312.1"
                     /translation="MSTAIWILLLIVALGVGVFGGIFIARKQIEKEIGEHPRLTPEAI
                     REMMSQMGQKPSEAKIQQTYRNIIKQSKAAVSKGKK"
     gene            2778..3572
                     /gene="racE"
                     /locus_tag="ACGEUI_000452"
     CDS             2778..3572
                     /gene="racE"
                     /locus_tag="ACGEUI_000452"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFF8165313.1"
                     /translation="MDTRPIGFLDSGVGGLTVVCELIRQLPHEKIVYIGDSARAPYGP
                     RPKKQIKEYTWELVNFLLTQNVKMIVFACNTATAVAWEEVKAALDIPVLGVVLPGASA
                     AIKSTTKGQVGVIGTPMTVASDIYRKKIQLLAPSIQVRSLACPKFVPIVESNEMCSSI
                     AKKIVYDSLAPLVGKIDTLVLGCTHYPLLRPIIQNVMGPSVKLIDSGAECVRDISVLL
                     NYFDINGNYHQKAVEHRFFTTANPEIFQEIASIWLKQKINVEHVTL"
     gene            3569..4555
                     /locus_tag="ACGEUI_000453"
     CDS             3569..4555
                     /locus_tag="ACGEUI_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HFF8165314.1"
                     /translation="MSEKIYEYKDENNWFIGKMTGHNLISGWGVKHTTIKKIDDLLDG
                     IAATLDWENPKGYDVSVVRHQSPLSLITFIIDMINQETQREIKVTPHAGTILLMENAK
                     LLAVYLPEGGVSTATFFATSEQGFGDIILIATRNEGKTKEFRNLFGQLGYRVENLNDY
                     PELPEVAETGTTFEENARLKAETISRLTGKMVLADDSGLKVDALGGLPGVWSARFSGP
                     DATDAKNNAKLLHELAMVFDQKKRSAQFHTTLVVAAPNKDSLVVEADWPGYIATQPKG
                     ENGFGYDPVFIVGETGHHAAELEADQKNQLSHRGQAVRKLMEVFPAWQAKQS"
     gene            4534..5055
                     /locus_tag="ACGEUI_000454"
     CDS             4534..5055
                     /locus_tag="ACGEUI_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8165315.1"
                     /translation="MASKTIIVMSDSHGDRDIVQAIKDKYLGQVDAIFHNGDSELNSS
                     DPIWAGIYVVGGNCDYDTGYPDRLVTQLGTVTIAQTHGHLYHINFTWDKLDYFAQEVV
                     ADICLYGHLHRPAAWQVGQTLFMNPGSVTQPRGEINEKLYARVELTDTQIKVDYFTRD
                     HKLYPSLSKEFKR"
     gene            5052..5513
                     /gene="cbpB"
                     /locus_tag="ACGEUI_000455"
     CDS             5052..5513
                     /gene="cbpB"
                     /locus_tag="ACGEUI_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFF8165316.1"
                     /translation="MIAKEFETFLMSHLDNYLIPEQDLAIFIDTHNADHVMLLLVSNG
                     FSRVPVITREKKYVGTISISDIMMYQSKRQLTDWEMSQTDIGEMVNTKIETISITSSL
                     TEIMHKLIDFPFLPVVDRANRFVGIITRKSILKAVNSLLHDFTDDYTIIKK"
     gene            5510..6256
                     /gene="xerD"
                     /locus_tag="ACGEUI_000456"
     CDS             5510..6256
                     /gene="xerD"
                     /locus_tag="ACGEUI_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFF8165317.1"
                     /translation="MKSYIEPFIASKALSQNSQKAYRYDLQQFCQLIGERVNQDKLLL
                     YQNSIANLSLSAKKRKLSTANQFLYYLYQIKYLNSYFRLTDTMKVMRTEKQQAAIINT
                     DIFYQKTPFVWGQLISLLILELGLTPSEVAGIEVANLDLNFQMLTLKTKKGVRVLPLS
                     QILIPFLEQQLVGKEVYLFEHRGIPFSRQWFFNHLKTFVRSIGYEGLTAQKLREQFIL
                     KEKLAGKSIIELSDILGLKSPMTLEKYYKS"
     gene            6256..6957
                     /locus_tag="ACGEUI_000457"
     CDS             6256..6957
                     /locus_tag="ACGEUI_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFF8165318.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSQYKVDIYEVPIVEVIEQYLNYIET
                     LQVMKLEVAGDYMLMASQLMLIKSRRLLPKVVEHIEEDLEQDLLEKIEEYSRFKAVSQ
                     ALAKQHDQRAKWYSKPKQELIFEDAILQEDKTVMDLFLAFSNIMAAKRAVLKNNHTVI
                     ERDDYKIEDMMASIKQRLEKENVIRLSAIFEECQTLNEVISIFLASLELIKLHVVFVE
                     QLSNFGAIILRKEKK"
     gene            6954..7505
                     /gene="scpB"
                     /locus_tag="ACGEUI_000458"
     CDS             6954..7505
                     /gene="scpB"
                     /locus_tag="ACGEUI_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFF8165319.1"
                     /translation="MTYLSQIEALLFVAGEEGLSLRHLASMLSLTPTALQQQLEKLSQ
                     KYEKDQHSSLCLIETANTYRLVTKEGFAELLRAYAKTPMNQSLSRASLEVLSIVAYKQ
                     PITRIEIDDIRGVNSSGALSKLLAFDLIREAGKKDVVGRPHLYATTDYFLDYMGINHL
                     DELIEVSAVEPADEEIALFRTQD"
     gene            7628..8350
                     /locus_tag="ACGEUI_000459"
     CDS             7628..8350
                     /locus_tag="ACGEUI_000459"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFF8165320.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSG
                     DVVEIEGSPIYNEEKVYYLLNKPRGAISSVSDDKGRKTVLDLLPQVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKP
                     ARYNIVRVEADKSRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRP
                     GEARRLNKKEISQLHNLANTKK"
     gene            8347..8610
                     /gene="yidD"
                     /locus_tag="ACGEUI_000460"
     CDS             8347..8610
                     /gene="yidD"
                     /locus_tag="ACGEUI_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFF8165321.1"
                     /translation="MMKKLLIVSVKAYQKYISPLSPPSCRYKPTCSAYMLTAIEKHGT
                     KGILMGIARILRCHPFVAGGVDPVPEDFSLMRNKNTSKNAEKA"
     gene            8788..9336
                     /locus_tag="ACGEUI_000461"
     CDS             8788..9336
                     /locus_tag="ACGEUI_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFF8165322.1"
                     /translation="MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAP
                     LHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLEQFINQCHGQLHLISKFAVNNYS
                     QATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYE
                     ALRQQGFQGLELKHTYEHDKLK"
     regulatory      9445..9568
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            9647..10210
                     /locus_tag="ACGEUI_000462"
     CDS             9647..10210
                     /locus_tag="ACGEUI_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8165323.1"
                     /translation="MSKTHKMIMIGILSAISFLLMLVSFAIIPGAAFLKIEFSIIPVL
                     FGLMIMDLKSAYLILLLRSLLKLFLNNRGVNDFIGLPMNIIAIALFVTAFALVWNRQK
                     TLSQYVFASLLGTGLLTFGMVVLNYTFAIPLYAIFANIDIRAYIGVTKYMMTMVIPFN
                     LVEGLIFAITFYFVYIASKPILERYLH"
     gene            10212..10865
                     /locus_tag="ACGEUI_000463"
     CDS             10212..10865
                     /locus_tag="ACGEUI_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFF8165324.1"
                     /translation="MMTNKQTHFLIASFALLIFVIIGYTVKFFPERLALLDNTIQAEI
                     RGNLPIVLTQFFRGVTVFGNVMTQVLLVIVSVLVLFFMKWKIEALFILSNGAIAAFLI
                     TTLKLFYQRPRPAIEHLVYAGGYSFPSGHAMGSMLIFGSLLIICYQRLHSKLLQFVTS
                     MIFIILILLIGLSRIYLGVHYPSDILAGFVLGFGILHFIYPFYKQKRFEWRFLLKQD"
     gene            11158..12078
                     /locus_tag="ACGEUI_000464"
     CDS             11158..12078
                     /locus_tag="ACGEUI_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407327.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFF8165325.1"
                     /translation="MKKRYQTLNEHYRQLFGAKMFKVPIDAGFDCPNRDGTVAHGGCT
                     FCTVSGSGDAIVAPDAPIKEQFYKEIDFMHRKWPDVNRYLVYFQNFTNTHDTVDVIRD
                     RYEQAINEPGVVGINIGTRPDCLPDDTIAYLAELSERMHVTVELGLQTTYEETSRLIN
                     RAHSYDLYKETVRRLRHYPNINIVSHLINGLPKETHDMMLENVRRCVTDNDIQGIKLH
                     LLHLMTNTRMQRDYHEGRLKLLSQKDYVSIICDQLEIIPKHIVIHRITGDAPRDMLIG
                     PMWSLNKWEVLNAIDKEMERRGSFQGCKVD"
     gene            12117..12671
                     /locus_tag="ACGEUI_000465"
     CDS             12117..12671
                     /locus_tag="ACGEUI_000465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8165326.1"
                     /translation="MLKRPIHLSHDFLAEVVDKSSVVVDATMGNGNDTAFLAQLAKKV
                     YAFDVQEQAIRKTSERLAQLGLSNAELILAGHEAVDQYVTEPVRAAIFNLGYLPSADK
                     SIITLPNTTLQALSKLLTLLMVGGRIAIMVYYGHDGGSLEKDALLDFVKQLDQRKVSA
                     MLYQPLNQVNTPPFLIMLEKLADF"
     gene            12804..14138
                     /locus_tag="ACGEUI_000466"
     CDS             12804..14138
                     /locus_tag="ACGEUI_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFF8165327.1"
                     /translation="MEDPVSQPLVIQFLLLVVLTLLNAFFSASEMALVSLNRSRVEQK
                     AADGDKKYARLLRVLEEPNHFLSTIQVGITFISLLSGASLSASLGKVISGWLGNSATA
                     RTAGTIISLVFLTYVSIVLGELYPKRIAMNLKDKLAIVSAPIIIGLGRLVSPFVWLLS
                     ASTNLLSRLTPMTFDDADEQMTRDEIEYMLSKSEATLDAEEIEMLQGVFSLDEMMARE
                     VMVPRTDAFMIDINDDPLENIQEILKQSFSRIPVYDVDKDKIIGLIHTKRLLESGFRQ
                     GFDQINMRKMLQEPLFVPETIFVDDLLRQLRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDKAEQFVHEIGDNTYIVVGTMTLNEFNDYFDTELESDDVDTIAGFYLT
                     GIGTIPSQEQKEAYEIDNKDKHLVLINDKVKDGRITKLKLILSNIEQIIEED"
     gene            14216..15007
                     /gene="pflA"
                     /locus_tag="ACGEUI_000467"
     CDS             14216..15007
                     /gene="pflA"
                     /locus_tag="ACGEUI_000467"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFF8165328.1"
                     /translation="MTEKDYGQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCH
                     NPDTWEMETNNSKIRTVNDVLKEALQYKHFWGKKGGITVSGGEAMLQIDFITALFIEA
                     KKLGIHTTLDTCGFTYRPTPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKN
                     ILQFARYLSDKQIPVWIRHVLVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGE
                     FKWRELGIPYQLEGVKPPTKERVQNAKNLMQTESYTEYMNRIHQS"
     gene            15138..16073
                     /locus_tag="ACGEUI_000468"
     CDS             15138..16073
                     /locus_tag="ACGEUI_000468"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFF8165329.1"
                     /translation="MSKILVFGHQNPDTDAIASSYAFDYLSQKAFGLDTEVVALGTPN
                     EETAFALDYFGVEAPRVVESAKAQGSEQVILTDHNEFQQSIADIREVEVYGVVDHHRV
                     ANFETANPLYMRVEPVGSASSIVYRMFKENGIEVPKAIAGMLLSGLISDTLLLKSPTT
                     HVSDHLVAEELAELAEVNLEDYGMALLKAGTNLASKSEVELIGIDAKTFELNGNAVRV
                     AQVNTVDIAEVLERQEAIEAAIKDAMAAEGYSDFVLMITDIVNSNSEILAIGANMDKV
                     EAAFNFTLDNNHAFLAGAVSRKKQVVPQLTESFGA"
     gene            16149..16802
                     /locus_tag="ACGEUI_000469"
     CDS             16149..16802
                     /locus_tag="ACGEUI_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFF8165330.1"
                     /translation="MITVFHSDKLTRQPFFQDLINYLDQHDHVILREIKKAFPNVTGI
                     DKAIESYVQAGYIRRENKRYGINLPLVSSDQQLALDTMLFVDTCSAMYENILAVVFET
                     QLTNQTNRVMIKEKTNITRDDLTLANYFYRLKRGEKPSAEQMDLYDLLGDVNQEYALK
                     YMTTFLLKFTRKDFVMQKRPDIFVEALVTLGYLKQVEPTTYQLLMTLDKESLTFIAP"
     gene            complement(16847..17848)
                     /locus_tag="ACGEUI_000470"
     CDS             complement(16847..17848)
                     /locus_tag="ACGEUI_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFF8165331.1"
                     /translation="MKSQRHLLRLYTSLILLLVSLMGLALSLGESHLSFLDLVHVFLG
                     KSSHAISFIVINIRLPRILAACLGGGSLALSGLLLQRLTRNPLADSGVLGITIGAGIS
                     LAIVVSFSFFEQAHISHYLPLFAMLGAIVTTFSVYWLSLTKQGQIDPTRLILTGVAVT
                     TMLSSLMVALVGHINRYKVDLVINWLSGQLIGDDWPTLSVIAPLLLCFWLLTYSQAHF
                     LNIMGLADNTAIGLGLPLNRKRRLILVLAAGLGALSVLLVGNISFIGLIAGHFSTYLV
                     GSNHKITIPISILIGMILLLVADTVGRVYLVGSNIQTGILVSLIGAPYFLYLMAKTK"
     gene            complement(17845..18897)
                     /locus_tag="ACGEUI_000471"
     CDS             complement(17845..18897)
                     /locus_tag="ACGEUI_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFF8165332.1"
                     /translation="MKTKHPFLCLERFCLKKTQIITKSHIFWLVFVLLSFSLCVAIYC
                     HLRFGAVALSHQDLNSILFGKQNGHKANVLLAIRLPRLFGATLTGSALAVSGTIMQAI
                     TRNPIAEPGLLGINAGAGLALVLAYAFVPHLHYSLIILLSLLGSSLAATLVFGLSYQS
                     GKGYHQLRLVLAGAMVSILLSALGQGITNYYHLANAVIGWQAGGLVGVNWQMIGYIAP
                     LIILSLCLAQLLSYHLTVLSLSESQAKALGQKTNLISAVFMILVLILSSAAVAIAGSI
                     SFIGLVIPHLMKHFTPHHYRYLLPLCAVSGASFMVWVDIACRNLNPPYETPLGALVSL
                     IGFPCFLWLIRRGGRY"
     gene            complement(18887..19819)
                     /locus_tag="ACGEUI_000472"
     CDS             complement(18887..19819)
                     /locus_tag="ACGEUI_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8165333.1"
                     /translation="MKKLTLLLTLCLTTITLIACGNQATNHSNTASKSLSPMPQIAGV
                     TYYGDIPKQPKRVVSLASTYTGYLKKLDMNLVGVTSYDKKNPILAKTVKKAKQVAATD
                     LEAVTTLKPDLIVVGSTEENIKQLAEIAPVISIEYRKRDYLQVLSDFGRIFNKEDKAK
                     KWLKDWKTKTAAYEKEVKAVTGDKATFTIMGLYEKDVYLFGKDWGRGGEIIHQAFHYD
                     APEKVKTEVFKQGYLSLSQEVLPDYIGDYVVVAAEDDKTGSALYESKLWQSIPAVKKH
                     HVIKVNANVFYFTDPLSLEYQLETLREAILSSEN"
     gene            complement(19845..20627)
                     /locus_tag="ACGEUI_000473"
     CDS             complement(19845..20627)
                     /locus_tag="ACGEUI_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8165334.1"
                     /translation="MTTISAEDLTIAYEQRTIIDKLSFYIPEGKITTIIGANGCGKSS
                     LLKALTRLLPPKQGVVYLNGQNIATLETKEVAKKLALLPQVQEATNGITVYELVSYGR
                     FPHQSYFGNLSPADKKAIHWAMQATNVMAYADQPVDALSGGQRQRVWLAMALAQGTDT
                     IFLDEPTTYLDLNHQLEILELVKSLNKDAGKTIVMVLHDLNLSARYSDHLIAMKHGKI
                     HYTGTIADVMTSPIIQDIFQIKPVLVDDPIHNCPIVLTYQLQ"
     gene            complement(20873..22318)
                     /locus_tag="ACGEUI_000474"
     CDS             complement(20873..22318)
                     /locus_tag="ACGEUI_000474"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFF8165335.1"
                     /translation="MITIEQLLDILKKDHNFREVLDADGYHYHYQGFSFERLSYDSRQ
                     VDGKTLFFAKGATFKADYLKEAITNGLQLYISEVDYELGIPVVLVTDIKKAMSLIAMA
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLT
                     TPESLDLFAMMAECVTNGMTHLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMENSRAVVINSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQA
                     FSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQKGIAKTRVPGRMEV
                     LTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIH
                     QHPNLTVILTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAV
                     IIAGKGADAYQIVKGQQVAYAGDLAIAKHYL"
     gene            22406..24040
                     /locus_tag="ACGEUI_000475"
     CDS             22406..24040
                     /locus_tag="ACGEUI_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8165336.1"
                     /translation="MSTEKKQLTQEELMVQGAAWSTAGNFISRLLGVLYIIPWYIWMG
                     QYAIQANALFNMGYNVYAYFLLISTTGLNVAIAKQVAKYNSMGQTEHSYQLIRSTLKL
                     MLGLGLIFSAIMYLGSPLFASLSGGDDTLVPIMHSLSLAVFIFPVMSVIRGIFQGHNN
                     IKPYAVSQIAEQLIRVIWMLLTTFFIMKLGSGDYASAVTQSTFAAFIGMVASMGVLGY
                     YLWKQGLLAAIFSKPDHTVSIDIKGLLLETLKESIPFIVTGSAIQAFQLIDQWTFVNT
                     MTLFTDYSRSQLLVLFGYFNANPAKITMVLIAVAASIGGVGIALLTENYVKKDMKAAA
                     RLIINNIEMLVMFLLPALTGAIILARPLYSVFYGASEERAIHLFVAVLFQTLLLALYT
                     LFSPMLQALFENRKAIYYFAYGILIKLVLQIPLIYLLHAYGPLLATTIALVVPIYLMY
                     RRLYQVTHFNRKLLQKRLLLTLIETLLMGLVVFVANWLLGYAFKPTGRLTSLLYLLII
                     GGLGMTVYTALTLLTHQLDKLIGSKASRLRQKLGWH"
     gene            24208..24837
                     /gene="upp"
                     /locus_tag="ACGEUI_000476"
     CDS             24208..24837
                     /gene="upp"
                     /locus_tag="ACGEUI_000476"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFF8165337.1"
                     /translation="MGKCQVISHPLIQHKLSILRRQTTSTKDFRELVNEIAMLMGYEV
                     SRDLPLEDVDIQTPVSKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRNEETLEPVEYLVKLPEDINQRQIFLVDPMLATGGSAILAVDSLKKRGAANIKFVCL
                     VAAPEGVKKLQEAHPDIDIFTAALDDHLNEHGYIVPGLGDAGDRLFGTK"
     gene            25061..25651
                     /locus_tag="ACGEUI_000477"
     CDS             25061..25651
                     /locus_tag="ACGEUI_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002274529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HFF8165338.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGTKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRHRLEKILAQNAGK
                     TIKQIHKDAERDYWMSAEETLAYGFIDEIMENNELK"
     gene            26143..26418
                     /locus_tag="ACGEUI_000478"
     CDS             26143..26418
                     /locus_tag="ACGEUI_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFF8165339.1"
                     /translation="MFQKQERIGLVVYLYYNRDARKLSKFGDLYYHSKRSRYLIIYIN
                     KNDLDTKLEEMRRLKCVKDIRPSAFDDIDRQFVGNLHRDETNNHQKG"
     gene            26667..27302
                     /gene="tmk"
                     /locus_tag="ACGEUI_000479"
     CDS             26667..27302
                     /gene="tmk"
                     /locus_tag="ACGEUI_000479"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFF8165340.1"
                     /translation="MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVAQDILTTREPGG
                     VAISEHIRELILDINHTAMDPKTELLLYIAARRQHLVEKVLPALEAGQLVFIDRFIDS
                     SVAYQGAGRGLIKADIQWLNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLD
                     LETIEIHQRVRKGYLALAKEHPKRIVTIDATKPLKEVVSVALEHVLALLLA"
     gene            27320..28195
                     /locus_tag="ACGEUI_000480"
     CDS             27320..28195
                     /locus_tag="ACGEUI_000480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFF8165341.1"
                     /translation="MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAK
                     VIFCEQKKDQTPCGHCRSCQLIEQGDFADVTVLEPTGQVIKTDVVKEMMANFSQTGYE
                     NKRQVFIIKDCDKMHINAANSLLKYIEEPQGEAYIFLLTNDDNKVLPTIKSRTQVFQF
                     PKNEAYLYQLAQEKGLLNHQAKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWL
                     KDQLQAYLALNRLVQLATEKEEQDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQS
                     NVNFQNTLEYMVMSE"
     gene            28214..28453
                     /locus_tag="ACGEUI_000481"
     CDS             28214..28453
                     /locus_tag="ACGEUI_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 C-terminal domain-containing protein"
                     /protein_id="HFF8165342.1"
                     /translation="MGKRSLSKDLPVATKVAAHKGKKAFEENTAFATDLMKEVSRLIT
                     QNQLEMTFVNSVFLLDQHVVLIPFSAEEHMDFYNC"
     gene            28858..29181
                     /gene="yabA"
                     /locus_tag="ACGEUI_000482"
     CDS             28858..29181
                     /gene="yabA"
                     /locus_tag="ACGEUI_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFF8165343.1"
                     /translation="MNKKELFDAFDGFSQNLMVTLAEIEAMKKQVQSLVEENTILRLE
                     NTKLRERLSHLEHETVAKNPSKQRKDHLEGIYDEGFHICNFFYGQRRENDEECMFCRE
                     LLDRK"
     gene            29186..30049
                     /gene="rsmI"
                     /locus_tag="ACGEUI_000483"
     CDS             29186..30049
                     /gene="rsmI"
                     /locus_tag="ACGEUI_000483"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFF8165344.1"
                     /translation="MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFIC
                     AEDTRNTGLLLKHFDIATKQISFHEHNAYEKIPDLIDLLISGRSLAQVSDAGMPSISD
                     PGHDLVKAAIDSDIAVVALPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFED
                     KHHYPETQMFYESPYRIKDTLTNMLACYGDRQVVLVRELTKLFEEYQRGSISEILSYL
                     EETPLKGECLLIVAGAQADSEVELTADVDLVSLVQKEIQAGAKPNQAIKTIAKAYQVN
                     RQELYQQFHDL"
     gene            30076..30468
                     /locus_tag="ACGEUI_000484"
     CDS             30076..30468
                     /locus_tag="ACGEUI_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HFF8165345.1"
                     /translation="MTEDQATILILGVYGIILAVFLLSALLCLIANWRLFKRAGYKGW
                     YSLIPFYAGFVKHKITFGEDNKWFYFIGWVIAIYYYYVHFCYVRAFGGSKGLAVFGLF
                     FPGIASLIVAFGKTYTTQECHPVPHLLN"
     gene            complement(30515..31144)
                     /locus_tag="ACGEUI_000485"
     CDS             complement(30515..31144)
                     /locus_tag="ACGEUI_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFF8165346.1"
                     /translation="MIKEFCAENLTLLPTLDAGQISRVELCDNLAVGGTTPSYGVIKE
                     ACQLLHDKKISVATMIRPRGGDFVYNDLELKAMEEDILKAVEAGSDALVLGLLTTENQ
                     LDTDAIEQLLPATQGLPLVFHMAFDRIPTDHQHQALDQLIDYGFVRVLTHGSPEATPI
                     TDNVEQLKSLVTYANKRIEIMIGGGITAENCQSLSQLTGTAIVHGTKII"
     gene            31443..31799
                     /locus_tag="ACGEUI_000486"
     CDS             31443..31799
                     /locus_tag="ACGEUI_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFF8165347.1"
                     /translation="MITIYQYPKCSTCQRAMAELKQLVSDFEAIDIKANPPKAQDLKH
                     WMETSGYTIKNFFNTSGNSYRELGLKDKIDQLSLDKAAELLATDGMLIKRPILIKDGN
                     VLQVGYRKPYQELDLG"
     gene            complement(31873..32700)
                     /locus_tag="ACGEUI_000487"
     CDS             complement(31873..32700)
                     /locus_tag="ACGEUI_000487"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFF8165348.1"
                     /translation="MKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQE
                     TKLSAKGPTKKHIETLLSYFPNYLHVWRSSVEPARKGYAGTMFLYKNTLNPVITFPEI
                     GAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQIWDHKYADYLTELDAQK
                     PVLATGDYNVAHKEIDLANPNSNRRSPGFTDEERQGFTNLLARGFTDTFRHVHGDIPH
                     VYTWWAQRSKTSKINNTGWRIDYWLASNRLVDKVKRSEMISSGERQDHTPILLDIDL"
     gene            32933..34114
                     /gene="lctO"
                     /locus_tag="ACGEUI_000488"
     CDS             32933..34114
                     /gene="lctO"
                     /locus_tag="ACGEUI_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HFF8165349.1"
                     /translation="MAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIP
                     KGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSLKGVENPSTEITFDGDYLTSPLILA
                     PVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYSKD
                     DGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGK
                     TMDYVYKSAKQALTSKDIEYIATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGG
                     RQLDGGPAAFDSLQEVAEAVDQKVPIVFDSGIRRGQHIFKALASGADLVALGRPAIYG
                     LAMGGSIGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRL
                     D"
     gene            34377..39320
                     /gene="scpC"
                     /locus_tag="ACGEUI_000489"
     CDS             34377..39320
                     /gene="scpC"
                     /locus_tag="ACGEUI_000489"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CXC chemokine-degrading serine protease SpyCEP"
                     /protein_id="HFF8165350.1"
                     /translation="MEKKQRFSLRKYKSGTFSVLIGSVFLVMTTTVAADELSTMSEPT
                     ITNHAQQQAQHLTNTELSSAESKSQDTSQITLKTNREKEQSQDLVSEPTTTELADTDA
                     ASMANTGSDATQKSASLPPVNTDVHDWVKTKGAWDKGYKGQGKVVAVIDTGIDPAHQS
                     MRISDVSTAKVKSKEDMLARQKAAGINYGSWINDKVVFAHNYVENSDNIKENQFEDFD
                     EDWENFEFDAEAEPKAIKKHKIYRPQSTQAPKETVIKTEETDGSHDIDWTQTDDDTKY
                     ESHGMHVTGIVAGNSKEAAATGERFLGIAPEAQVMFMRVFANDIMGSAESLFIKAIED
                     AVALGADVINLSLGTANGAQLSGSKPLMEAIEKAKKAGVSVVVAAGNERVYGSDHDDP
                     LATNPDYGLVGSPSTGRTPTSVAAINSKWVIQRLMTVKELENRADLNHGKAIYSESVD
                     FKDIKDSLGYDKSHQFAYVKESTDAGYNAQDVKGKIALIERDPNKTYDEMIALAKKHG
                     ALGVLIFNNKPGQSNRSMRLTANGMGIPSAFISHEFGKAMSQLNGNGTGSLEFDSVVS
                     KAPSQKGNEMNHFSNWGLTSDGYLKPDITAPGGDIYSTYNDNHYGSQTGTSMASPQIA
                     GASLLVKQYLEKTQPNLPKEKIADIVKNLLMSNAQIHVNPETKTTTSPRQQGAGLLNI
                     DGAVTSGLYVTGKDNYGSISLGNITDTMTFDVTVHNLSNKDKTLRYDTELLTDHVDPQ
                     KGRFTLTSHSLKTYQGGEVTVPANGKVTVRVTMDVSQFTKELTKQMPNGYYLEGFVRF
                     RDSQDDQLNRVNIPFVGFKGQFENLAVAEESIYRLKSQGKTGFYFDESGPKDDIYVGK
                     HFTGLVTLGSETNVSTKTISDNGLHTLGTFKNADGKFILEKNAQGNPVLAISPNGDNN
                     QDFAAFKGVFLRKYQGLKASVYHASDKEHKNPLWVSPESFKGDKNFNSDIRFAKSTTL
                     LGTAFSGKSLTGAELPDGHYHYVVSYYPDVVGAKRQEMTFDMILDRQKPVLSQATFDP
                     ETNRFKPEPLKDRGLAGVRKDSAFYLERKDNKPYTVTINDSYKYVSVEDNKTFVERQA
                     DGSFILPLDKAKLGDFYYMVEDFAGNVAIAKLGDHLPQTLGKTPIKLKLTDGNYQTKE
                     TLKDNLEMTQSDTGLVTNQAQLAVVHRNQPQSQLTKMNQDFFISPNEDGNKDFVAFKG
                     LKNNVYNDLTVNVYAKDDHQKQTPIWSSQAGASVSAIESTAWYGITARGSKVMPGDYQ
                     YVVTYRDEHGKEHQKQYTISVNDKKPMITQGRFDTINGVDHFTPDKTKALGSSGIVRE
                     EVFYLAKKNGRKFDVTEGKDGITVSDNKVYIPKNPDGSYTISKRDGVTLSDYYYLVED
                     RAGNVSFATLRDLKAVGKDKAVVNFGLDLPVPEDKQIVNFTYLVRDADGKPIENLEYY
                     NNSGNSLILPYGKYTVELLTYDTNAAKLESDKIVSFTLSADNNFQQVTFKITMLATSQ
                     ITAHFDHLLPEGSRVSLKTAQDQLIPLEQSLYVPKAYGKTVQEGTYEVVVSLPKGYRI
                     EGNTKVNTLPNEVHELSLRLVKVGDASDSTGDHKVMSKNNSQALTASATPTKSTTSAT
                     AKALPSTGEKMGLKLRIVGLVLLGLTCVFSRKKSTKD"
     gene            40091..40798
                     /locus_tag="ACGEUI_000490"
     CDS             40091..40798
                     /locus_tag="ACGEUI_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFF8165351.1"
                     /translation="MSKKKRKHYQVYEGLRCAMTLCFISGYVNAFTYMTQGKRFAGVQ
                     TGNLLSFAIRLSEQQLKEALQFLLPMIVFMLGQSFTYFMHRWATKKGLHWYLLSSVIL
                     TGIAFGTALFTPFLPSNVTVAALAFFASIQVDTFKTLRGASYANVMMTGNIKNAAYLL
                     TKGLYEKNHELTHIGRNTLIVILAFAVGVVCSTLLCIAYGEYALMPILMPLLYVNYLL
                     AQEFYHIQTKIKPIKRT"
     gene            41041..43038
                     /gene="metG"
                     /locus_tag="ACGEUI_000491"
     CDS             41041..43038
                     /gene="metG"
                     /locus_tag="ACGEUI_000491"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFF8165352.1"
                     /translation="MKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVF
                     YLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHEE
                     VVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGHE
                     VEWVSEESYFLRLSKYDDRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTT
                     FTWGVPVPSDPKHVVYVWIDALLNYATALGYRQANHANFDKFWNGTVFHMVGKDILRF
                     HSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRYY
                     LMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGTA
                     FDADLSQLIDAQLADYHKHMEAVDYPRALEAVWTIIARTNKYIDETAPWVLAKEDGDK
                     AQLASVMAHLAASLRLVAHVIQPFMMETSAAIMAQLGLEPVSDLSTLALADFPANTKV
                     VAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITFETFDAV
                     EIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKFYPNEQELVGKKLQIVAN
                     LKPRKMMKKYISQGMILSAEHGDQLTVLTVDSSVPNGSIIG"
     gene            43533..44546
                     /gene="nrdF"
                     /locus_tag="ACGEUI_000492"
     CDS             43533..44546
                     /gene="nrdF"
                     /locus_tag="ACGEUI_000492"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFF8165353.1"
                     /translation="MTQHYYERSQSPIEYALSETQKQLRSINWNYLNDDKDLEVWNRV
                     TQNFWLPEKVPVSNDLNSWRSLGEDWQQLITRTYTGLTLLDTVQATVGDVAQIQHSQT
                     DHEQVIYTNFAFMVGIHARSYGTIFSTLCSSEQIEEAHEWVVSTQSLQDRARVLIPYY
                     TGDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKMPNTSDIIRLILRDKVIHNYYSG
                     YKYQQKVARLSPEKQAEMKAFVFDLLYELIDLEKAYLRELYAGFDLAEDAIRFSLYNA
                     GKFLQNLGYESPFTDEETRVSPEVFAQLSARADENHDFFSGNGSSYVMGITEETTDDD
                     WEF"
     gene            44550..45038
                     /gene="nrdI"
                     /locus_tag="ACGEUI_000493"
     CDS             44550..45038
                     /gene="nrdI"
                     /locus_tag="ACGEUI_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFF8165354.1"
                     /translation="MAELIIVYFSSKSNNTHRFVQKLGLPAQRIPVDNRPLEVSTHYL
                     LIVPTYAAGGSDAKGAVSKQVIRFLNNPNNRKHCKGVISSGNTNFGDTFALAGPIISQ
                     KLQVPLLHQFELLGTATDVKKVQAIFARLKHHTHDKQKQTNNLITERTHPCHKPMRHT
                     SH"
     gene            45005..47185
                     /gene="nrdE"
                     /locus_tag="ACGEUI_000494"
     CDS             45005..47185
                     /gene="nrdE"
                     /locus_tag="ACGEUI_000494"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFF8165355.1"
                     /translation="MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPN
                     QMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQ
                     SYALKTLDGKQYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAG
                     KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIEN
                     QATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIK
                     SLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISITEEYDKLLANPAIKK
                     TYISARKFFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPS
                     TFKEDLSFETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAP
                     SVETGNAANHAVGLGAMNLHGFLATNHIYYDTKEAVDFTDLFFHAMAYYAFKASCQLA
                     KEKGAFAGFSLSTYSDGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADWQALVAD
                     IKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQA
                     NYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQN
                     CASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTECQSCMI"
     gene            complement(47388..48140)
                     /locus_tag="ACGEUI_000495"
     CDS             complement(47388..48140)
                     /locus_tag="ACGEUI_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HFF8165356.1"
                     /translation="MLKKRYQLAIVLLLSCFSLIWQTEGLVELFVCEHYERAVCEGTP
                     AYFTFSDQKGAETLIKKRWGKGLIYPRAEQEAMAAYTCQQAGPINTSLDKAKGELSQL
                     TPELRDQVAQLDAATHRLVIPWNIVVYRYVYETFLRDIGVSHADLTSYYRNHQFDPHI
                     LCKIKLGTRYTKHSFMSTTALKNGAMTHRPVEVRICVKKGAKAAFVEPYSAVPSEVEL
                     LFPRGCQLEVVGAYVSQDQKKLHIEAYFKGSL"
     gene            48599..49135
                     /locus_tag="ACGEUI_000496"
     CDS             48599..49135
                     /locus_tag="ACGEUI_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165357.1"
                     /translation="MKTKSKRFLNLATLCLALLGTTLLMAHPVQAEVISKRDYMTRFG
                     LGDLEDDSANYPSNLEARYKGYLEGYEKGLKGDDIPERPKIQVPEDVQPSDHGDYRDG
                     YEEGFGEGQHKRDPLETEAEDDSQGGRQEGRQGHQEGADSSDLNVEESDGLSVIDEVV
                     GVIYQAFSTIWTYLSGLF"
     gene            49409..49660
                     /locus_tag="ACGEUI_000497"
     CDS             49409..49660
                     /locus_tag="ACGEUI_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165358.1"
                     /translation="MPEKKVLPVLSIVVSFVCLLDHIFFFATYSYLYLPLVASSLALI
                     GLVQNKGRQKVWSLAGLVLAALAVAVVAYAYYDPTVIPY"
     gene            complement(49754..50386)
                     /locus_tag="ACGEUI_000498"
     CDS             complement(49754..50386)
                     /locus_tag="ACGEUI_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165359.1"
                     /translation="MKKTLTLLLALFAIGVTSSVRAEDEQSSTQKPVKFDLDGPQQKI
                     KDYSGNTITLEDLYVGSKVVKIYIPQGWWVYLYRQCDHNSKERGILASPILEKNITKT
                     DPYRQYYTGVPYILNLGEDPLKKGEKLTFSFKGEDGFYVGSYIYRDSDTIKKEKEAEE
                     ALQKKEEEKQQKQLEESMLKQIREEDHKPWHQRLSESIQDQWWNFKGLFQ"
BASE COUNT        15912 a         9721 c        10479 g        14848 t
ORIGIN      
        1 gtctacttcc tgtcactttt actaaagaca agaatacaaa aatgagatag ttagtagtac
       61 taacagtaaa agaggtttgt ctattttaaa aatagtatca aaaaaaggtc ttttaggttt
      121 aaaacattga actgacccca aattgaccat ctaagaagtt gatgctttca aatgctgtcg
      181 cgtatggtat aataggcgca tggttattac atcaaaagca aatcaactaa ttaagcaaac
      241 aaaaaaatta ttgcaaaaaa agcatcgaaa acagtcctat ttgattgagg gctggcacct
      301 ttttgaagaa gcccaaaaat caggacaagt ttttcgccac atctttgttt tagaggaaat
      361 ggtggagcgt ctagcaggtg agcaagaact cgttattgtt agcccacaag tgctcaagga
      421 gttgactgac tctcctagtc cacaaggcat tgttgctgag gtggagatac ctaagttagc
      481 ctttccatca gattacaaag ggaaatattt ggttttagag gacgtccaag acccaggaaa
      541 tcttgggacc atcattcgta cagcagacgc tgcacggttt gatggggtct ttctttctga
      601 aaaatcagca gatatttaca accaaaaaac cctacgttcc atgcaaggta gccattttca
      661 tttaccaatt tggcgaacag atgtctatca gctttgtcga gagctccaag agtatgaaac
      721 tcccatccta gcaacaactc tgtcaaagaa atctgttgat tataaaagcc tcacacatca
      781 tgaaaggtta gctttggtgt tgggaaatga aggccagggc attagtgctg aaatggcagc
      841 cctagcagac caactggttc atattacaat gcctggtcag gcggaaagtt tgaatgttgc
      901 cgtagcagca ggaatcctca ttttcagctt gatttaagct aatatgatat aataaccaag
      961 tgaggtaatt atgttatgca ttatacagaa gataaagaat atatggaaca cgtcggtcat
     1021 ttgatcgctc atcctcgctt tcaaaaattg agtcatattg ttcagcacca acattcaacg
     1081 cgtcttgaac attctatcaa tgtttcttat tcaagttata aattagccaa gcgattcggt
     1141 tgggatgcta aaagtacagc cagagggggc ttacttcatg atttctttta ttatgattgg
     1201 cgcgtgacca agtttaataa gggtcatgcc tgggttcatc ctcgtattgc tgtacgaaat
     1261 gcgaaaaagt taacagaact caataaaaaa gaagaggata tcattctaaa acacatgtgg
     1321 ggagcgacca ttgcttttcc gcgttacaag gaaagttata ttgtgacaat ggtagataaa
     1381 tactgggctg tcaaggaagc agtgacgcct ttgagacaaa aatggtctaa tcgacggttt
     1441 cttcgccgca agaccctcca aagtcataat cgttaagtaa aggagttatt atgaacgatc
     1501 acgttattta tacacaatca gatgtgggat taaatcagtt ttttgctaaa atttatagtc
     1561 tagtgggaat gggagtgggt ttgtctgctt ttgtttctta tttgatgctt tatccattta
     1621 gagaaaattt aatctctatt cttgttaatc agccgatgat ttactatggt gctgcgatta
     1681 ttgaacttat tttagttttt gtagccagta gtgcagcacg aaaaaacaca ccagctgcat
     1741 tgcccatttt tcttatctat tctgcgctaa atggctttac cctaagcttt attattgtgg
     1801 catacgctca aacgacagtt tttcaagcat ttctttcttc agcagctgtg ttttttgcca
     1861 tgtcaatcat tggtgtcaag actaaacggg atatgtcagg tcttagaaag gctatgtttg
     1921 ctgctttgat tggtgttgtt gtggctagtc tgattaatct ttttattggt agtgggatga
     1981 tgagttatgt cattagtgtg atttccgtgc taattttctc aggtctcatc gcatcagata
     2041 atcaaatgat taagcgcgtt tatcaagcaa caaatggcca ggttggtgat ggctgggctg
     2101 ttgctatggc cttaagcttg tatttagatt ttattaactt atttatcagc cttcttcgta
     2161 tttttggaag aaatgattaa cattaaaaag tgagtataac tactcacttt ttaatgttaa
     2221 caggataagt aaactgtaag gaagaaattc gagatagata taagatgagg ctgctaaaag
     2281 acctagtgtt aatttgtaaa ctctttcttt ttttggtata ctattaagga ttaaatacga
     2341 ggagaatata gatagatatg tcaacagcta tttggatttt attacttatt gttgcccttg
     2401 gggtaggtgt ttttggtggg atttttattg cccgtaaaca aattgaaaaa gaaattggtg
     2461 aacacccacg tttaacacca gaagctatcc gtgaaatgat gagccagatg gggcaaaaac
     2521 caagtgaagc aaaaatccaa caaacttatc gtaatatcat caaacaatca aaagcagccg
     2581 tatccaaagg caaaaaataa aagctaattg ttatagggga aacctttgtg atagttagaa
     2641 gtgaacagtc tttgatctca atgttcagta gcacttgtct tatcagttag atttgtcaac
     2701 tgttcagggt ttaaaaagaa actcagttgg ctatttccaa ctgagttttt catatcgtca
     2761 gtaaggaagt aaacataatg gatacaagac caattgggtt tttagattct ggtgtgggag
     2821 gtctgaccgt cgtttgtgaa ttgattcgtc agctccctca tgagaagatt gtatatattg
     2881 gtgattcagc aagagccccc tatggtccta gacccaaaaa acaaattaag gagtatactt
     2941 gggagctagt caattttttg ctgactcaaa atgttaaaat gattgttttt gcatgcaata
     3001 ccgcaacagc ggtggcttgg gaagaagtaa aagcagcttt agatattcct gttttaggag
     3061 ttgtcttacc gggggcaagc gcagctatta aatcaacgac aaaaggccag gttggggtca
     3121 tcggaacccc aatgacagtg gcttcagaca tttatcgcaa aaaaatccag ctattagcac
     3181 catctattca agtaaggagt cttgcttgcc cgaagtttgt accgattgtg gaatcaaatg
     3241 agatgtgttc gagtatagct aaaaaaatag tttatgacag tctagcacca ttagtcggta
     3301 aaatagatac ccttgtacta ggatgtactc actatccctt gttacgacca attatccaaa
     3361 atgttatggg gccatctgtt aagctgattg acagtggagc agaatgcgtc cgagatatct
     3421 ctgtcttatt aaactatttt gacattaacg gtaattatca tcaaaaagct gtcgaacacc
     3481 gcttttttac aacagcaaat cctgagattt ttcaagaaat tgcctcgatt tggctgaaac
     3541 aaaaaataaa tgtggagcat gtcaccttat gagtgaaaaa atttacgaat acaaagatga
     3601 aaataactgg tttatcggaa agatgactgg tcataattta atatcgggct ggggagtcaa
     3661 acacacgaca atcaaaaaaa tagatgacct tttagacggt attgccgcaa cgttagattg
     3721 ggaaaatcct aaaggttacg atgtttcggt ggtgcgtcat cagtctcctt tatctctcat
     3781 cacctttatt atcgacatga taaatcaaga gacccagcgt gagattaagg taactcccca
     3841 tgcaggcacg attttattaa tggaaaatgc gaaactgctt gcagtttatc tcccagaagg
     3901 tggagtttca acggctacat tttttgcgac tagtgaacag ggatttgggg atattatttt
     3961 aatcgctaca cgaaacgagg gaaaaaccaa agagttccgc aatctgtttg gccagctagg
     4021 ttatcgtgtg gaaaatctta atgattatcc agaattgcca gaagtagcag aaactggtac
     4081 tacttttgaa gaaaatgctc gcttgaaagc agaaacgatt tcacgcctca caggaaaaat
     4141 ggtattagca gacgattcag gactaaaagt tgatgccttg ggtggtttac ctggtgtttg
     4201 gtcagctcgt ttctcaggac cagatgccac agatgccaag aataatgcca aactcttaca
     4261 cgaattagct atggtgtttg accaaaagaa gcgctcagct caatttcata cgactttggt
     4321 agtggctgct cccaataaag atagtttggt tgtggaagcc gattggccag gctatattgc
     4381 tacgcagcct aagggagaaa atggttttgg gtatgatcct gtctttatcg tcggagagac
     4441 tgggcatcat gctgcagaat tagaggctga ccaaaaaaat cagttgtcgc atagaggtca
     4501 agcagttcga aaattaatgg aggtctttcc agcatggcaa gcaaaacaat catagtaatg
     4561 agtgactctc atggtgaccg agatatcgtt caagccatta aagacaagta tcttggtcaa
     4621 gttgatgcta ttttccataa tggggattct gaattaaaca gctcagatcc tatttgggct
     4681 ggtatttatg tcgttggcgg taattgtgac tatgatacag gttaccctga tcgtttagta
     4741 actcaattag gcacagttac gattgcccag acacatggtc acttgtacca tattaatttc
     4801 acttgggata aactggacta ctttgctcaa gaagtagtag cagatatttg tctatatgga
     4861 cacctacacc gtccagctgc ttggcaagtt gggcaaaccc tctttatgaa tcccggatca
     4921 gtaacacaac ctcgcggaga aattaacgag aaattatatg ctcgtgttga actaactgat
     4981 actcaaatta aagttgatta ctttaccaga gatcataaac tttacccatc actgtctaag
     5041 gaatttaaac gatgatcgca aaagaatttg aaacattttt aatgagtcat ttagataatt
     5101 atcttatccc ggaacaagac ttggctattt ttatcgacac acataatgca gaccatgtga
     5161 tgttactatt ggtgagcaat ggtttttcta gagttcctgt tatcacacgg gagaaaaaat
     5221 atgtgggaac cattagcatc tcagatatta tgatgtacca atctaaaagg cagctgacag
     5281 attgggaaat gagtcaaaca gatattggcg agatggttaa taccaaaatt gaaaccatta
     5341 gcattacttc tagcctaaca gaaattatgc acaagttaat tgatttccca ttcttaccgg
     5401 ttgtagatag agcgaatcgc tttgttggga ttattacgcg aaaatctata ttaaaggctg
     5461 tcaacagtct tttacatgat tttacagatg attacacgat tataaaaaaa tgaagagtta
     5521 tattgaacct tttattgcta gcaaggcttt atctcaaaat agtcaaaaag cttatcgata
     5581 cgatttgcaa cagttttgtc aactgattgg tgaacgtgtc aatcaagaca aactattact
     5641 ttatcaaaat agcatagcta acctaagttt atctgcaaaa aaacgcaaat tatcaacagc
     5701 taatcaattc ttatattact tgtatcaaat aaagtattta aatagctatt ttcgattaac
     5761 agacaccatg aaagtgatgc ggactgagaa gcagcaagct gctattatta atactgatat
     5821 tttctatcaa aaaacccctt ttgtgtgggg gcaattaatc agcttattaa tattggagct
     5881 gggtttaact ccgagtgaag tagctggtat tgaagtagct aacttggatt taaattttca
     5941 aatgttgacg ctaaagacta aaaaaggtgt gcgtgtatta cctttatcgc aaatactaat
     6001 ccctttttta gaacaacaac tcgttggcaa agaagtctat ctttttgaac atcgaggcat
     6061 ccctttttca agacaatggt tttttaatca cctaaaaacc tttgttcgaa gtattggtta
     6121 tgaaggattg actgctcaaa aattacgaga gcaatttatt ttgaaagaaa aactagctgg
     6181 taaatcgata atagaactta gcgatattct tgggctgaaa agccctatga ccttagaaaa
     6241 atactacaaa tcataatgga tataaaactt aaagattttg aaggtcctct tgatttactc
     6301 ttacacttgg tatcacagta caaagttgac atctacgagg ttcctattgt tgaggttatt
     6361 gagcagtacc ttaattatat tgaaacctta caggtaatga aacttgaagt ggctggtgac
     6421 tacatgctga tggcaagtca gctcatgtta atcaaaagta gaaggctatt accaaaggtg
     6481 gtagaacaca tcgaagaaga tttagaacaa gatctgttag aaaagatcga agaatatagt
     6541 cgctttaaag cagttagtca agcgttagcg aagcaacatg accaaagggc aaaatggtat
     6601 tcaaaaccta aacaagaatt aatttttgaa gatgctatct tacaggaaga taaaacggtg
     6661 atggatctat ttctagcttt ttcaaacata atggcagcta aaagagcagt tttaaaaaac
     6721 aaccatacag tcatcgagcg tgatgattat aagattgaag acatgatggc ttctattaaa
     6781 cagcgactgg aaaaagaaaa cgttattaga ttatcagcga tttttgagga atgccaaaca
     6841 ttaaatgaag ttatcagtat atttttagct agtctagaat tgattaagct tcacgtggta
     6901 tttgtagagc agctctctaa ttttggggct atcatactac gaaaggagaa gaaatgacgt
     6961 atttgtcaca gatagaagcg ctgctatttg ttgcaggtga agaaggatta agtttgcgac
     7021 atctagcgtc tatgttatca ttaacgccaa cagctcttca acagcaatta gaaaaactct
     7081 ctcaaaagta tgaaaaagat caacattcta gtctttgctt gatagaaaca gctaatacct
     7141 acaggctagt aactaaagaa ggttttgcag aactcttgcg tgcttatgcc aaaacaccaa
     7201 tgaaccaaag tttatctcgt gcgagtttag aggtcttgtc tattgttgca tacaaacaac
     7261 ctattacacg aatagaaatt gatgacattc gaggggtaaa ctccagtgga gctttgagta
     7321 aattattggc atttgatctc attagagaag ctggcaaaaa agatgtggtt gggcgtcctc
     7381 atttgtatgc gacaacagat tattttctgg actatatggg aattaatcat ttagacgaat
     7441 tgattgaagt atctgccgtt gaaccagcag atgaagagat tgctcttttt aggacacaag
     7501 actgagtttt gcttgtcatc aaatagcaaa tggcgtttac aaaaggattt gttttaaggc
     7561 gttttagttg aaaaaaaagc cttaaactag ggacaacaaa aagttgctca aaagaaagaa
     7621 gaatataatg cgaattaaca aatacattgc ccatgcgggt attgccagtc gtcgtaaggc
     7681 tgaagaattg attaagcaag gcttagtaac cttaaatggt caggttatta cggatttagc
     7741 aaccactgta aaatcgggag acgtggttga aatagaagga agtcctattt acaacgagga
     7801 aaaggtttac tatttgctta ataaacctcg tggcgctatc tcaagtgtgt cagatgacaa
     7861 aggccgtaaa acggtactgg atttactacc tcaggttaaa gaacgcatat accctgttgg
     7921 acgattggat tgggacacat caggggtcct tattttaacg aatgatggtg attttacaga
     7981 tactatgatt catcctagaa atgaaataga caaggtttat ctggcgcgtg tcaaaggtat
     8041 tgcaacgaaa gaaaatcttc gtcccttaac tcgaggaatt gttattgatg gtaaaaagac
     8101 caaaccagct cgctacaata ttgttcgtgt agaagctgat aagagtcgat cgatcgtaga
     8161 attgactatc catgaagggc gaaaccatca agtgaaaaaa atgtttgagt ctgtggggct
     8221 tctggttgat aaattatctc gtacacgttt tgggaccgtc gacttaaagg gactaagacc
     8281 aggagaagca agacgcttaa ataaaaaaga aataagtcaa ctgcataact tagctaatac
     8341 gaaaaaatga tgaaaaaact attaatagta tctgttaaag cttatcaaaa atacatttct
     8401 cctttatctc ctcctagttg tcgctacaag ccaacctgtt cagcttatat gttaactgct
     8461 attgaaaaac atggtactaa gggaatatta atggggatag caagaatatt aagatgtcac
     8521 ccttttgttg ctggtggtgt cgatcctgtt ccagaagatt ttagcctaat gagaaataaa
     8581 aacacgtcta aaaacgctga aaaggcatga ctttatcaaa aaatggcttg acattgatag
     8641 ctgaagtgtt ataatggaaa ataagcattc gttggtttaa atcaaacctg ttatgattta
     8701 agttagcgag tcaccatcaa ccacattgtt tgctgagcat gactccgagc agtgtggttt
     8761 tttaaattaa aaagagtaaa tgagattatg acgacaaaag aacttatcaa caaaaacgac
     8821 aaagtaaaaa aggctcgcaa tcatattgtc ctgtttcaac cccaaattcc acaaaatact
     8881 ggtaatattg ctagaacatg tgctgcaact aatgcacctt tacatattat taaacccatg
     8941 ggatttccta ttgatgaccg caaaatgaag cgtgcaggct tggattactg ggacaagtta
     9001 gaactgcatt tttatgatca tttagaacag tttatcaatc aatgtcacgg acagcttcac
     9061 ctgattagca agtttgcagt taataattat tctcaagcta cttatgctga tggcgattcc
     9121 cattatttct tgtttggacg agaagataca ggcctgccag aagattttat gcgagaacat
     9181 gctgaaaaag cacttcgtat tccgatgaat gatgagcatg tacggagttt aaatgtctca
     9241 aatacggttt gtatggtgat ttatgaagcc cttagacaac agggctttca agggttagag
     9301 ttaaagcata cttacgaaca cgataaacta aaataatgga ctttttaaaa gcgtcagttt
     9361 ttggcaacat gcgttgtcag tagctgacgc ttttaaaaat ttagtcaaag ctattgctat
     9421 ctaggtttaa atctgctatg ctagaagtgt cttcagggca gggtgtgatt cccgaccggc
     9481 ggtgactagt aactagaagt ccgcgagcgc aagctgatgt ggtgtaactc cacaaccgac
     9541 agtatagtct ggatgagaga agaccgggct ttttgtgtag gcttatttta gctacacaat
     9601 aacgacggaa cttctttcaa tttgaaaaaa ttggaggaat tttttaatgt caaaaacaca
     9661 taaaatgatt atgattggta tactttctgc gatatcattt ctattgatgc tagttagctt
     9721 tgccattatt ccaggcgcag cttttttaaa aattgaattt agtattattc ctgttttatt
     9781 tggtttaatg attatggact taaagagtgc ttacttaatt ttgttattga ggtccttatt
     9841 gaaactcttt ttgaacaatc gtggggttaa tgattttatt ggtctcccaa tgaatattat
     9901 cgctatcgct ttatttgtga cggcttttgc cttagtttgg aaccgtcaaa aaacgcttag
     9961 tcaatatgta tttgctagct tactaggcac agggttatta acgtttggca tggttgttct
    10021 taattatact tttgccattc ctttatatgc tatatttgca aatattgata tcagagctta
    10081 tattggtgtt actaagtata tgatgactat ggttattccg tttaatcttg ttgaagggtt
    10141 gatatttgca attacctttt attttgtgta tattgcaagt aaaccaattt tagaaagata
    10201 cttacactaa tatgatgaca aacaaacaaa ctcacttttt aatagcttct tttgcattgc
    10261 taatctttgt tataataggc tatacggtca aattttttcc tgaacgtttg gcacttctag
    10321 ataatactat tcaagcagag attagaggca accttcctat agtgctgacg caattcttta
    10381 ggggtgttac tgtatttgga aatgtcatga cacaagtttt gttagttatt gtgtcggtcc
    10441 tagttttatt ctttatgaag tggaaaattg aagccttgtt tatcttgtca aatggtgcca
    10501 ttgcagcatt tttgattacg actctcaagc tcttttacca aagaccaaga cctgctattg
    10561 agcatttggt ttatgctggt ggctattcgt ttcccagtgg ccatgcgatg ggtagcatgc
    10621 tgatttttgg tagcctattg attatttgtt atcagaggct acattcaaaa ctattacaat
    10681 ttgtgactag catgatcttt ataatactaa ttttattaat tgggttatct cgtatttatc
    10741 taggagtaca ctacccaagt gatattttag caggctttgt attaggattt ggtattttgc
    10801 actttattta tcctttttat aagcaaaaac gatttgaatg gcgatttctt ttgaaacaag
    10861 actaattttt cttgagaaga cataggctta tttaaatcat ttagaataac attggcaagc
    10921 atcaccccct tgattgccag tgttatttta gttttagtta gatgatgtga gctgatgatt
    10981 agtaaatatg tttagataaa aaacgattaa agtgctatgt aacgtcagaa aagacgtgat
    11041 ttttgttgat ctgtaatcat taagtaagct gatgtagaag tagtgaattc taatgactga
    11101 gctatctttt gcatagatga ctatcttgct agtaatgatt tgtgctacaa tagaaacatg
    11161 aaaaaaagat atcagacatt aaatgagcat taccgtcaac tatttggcgc aaaaatgttt
    11221 aaagttccga ttgatgccgg ctttgattgt cccaatcgtg atggaacggt agctcatgga
    11281 ggatgtactt tttgcacagt atcaggttct ggagatgcta ttgtagcacc agatgctcca
    11341 attaaagagc aattttataa agaaattgat tttatgcacc gtaaatggcc tgatgtcaat
    11401 cgctatttgg tttattttca aaactttacc aatactcatg atacagttga tgtgattcgc
    11461 gaccgttacg aacaagctat taacgaacct ggagttgttg gtattaatat tggcacacgg
    11521 cctgactgtc taccagatga taccattgct tacttagcag aattatcaga acgcatgcat
    11581 gtgacagtgg agctaggctt acagacaact tatgaagaaa cttctaggct aattaacaga
    11641 gcacattctt atgatttgta taaagaaacg gtgaggcgac taaggcatta ccccaacatt
    11701 aatattgtct cgcacttgat taatggattg ccaaaagaaa ctcatgacat gatgcttgaa
    11761 aatgtgagac gctgtgtaac agacaatgac attcaaggaa ttaaattgca tttgctgcat
    11821 ttgatgacca atactagaat gcaaagagat tatcatgaag gacgcttaaa gttgttgagt
    11881 caaaaggact atgtttcgat tatttgtgac cagctagaga ttatccctaa gcatattgtg
    11941 attcaccgta ttacaggaga tgccccacgc gatatgttga ttgggcccat gtggagtcta
    12001 aataagtggg aagtattgaa tgccattgat aaagaaatgg aaagacgagg aagctttcag
    12061 ggctgtaaag ttgactaatt tgtctcattt tgacggattt ggataggaga aaagtgatgc
    12121 taaaacgccc cattcatctc tcgcatgatt ttttagcgga ggttgtggat aagtctagtg
    12181 tggttgttga tgccacgatg ggtaatggaa atgatactgc ctttttggca caattagcta
    12241 aaaaggttta tgcttttgat gtgcaagaac aagctattag aaaaacaagt gagcggttag
    12301 cgcagctagg attatcgaac gctgagctga ttttagcggg tcatgaagcg gttgatcagt
    12361 atgtgacaga gccggtgcgc gcagcgattt ttaaccttgg ttatttgccc agcgcagata
    12421 aatctattat taccttacca aataccacac tccaagcttt atcaaaacta ctaacgttat
    12481 taatggtagg tgggcgtatt gctattatgg tctattatgg gcatgatgga ggcagtttgg
    12541 aaaaagatgc tttattagat tttgtcaagc aattagacca aagaaaggtt tctgcaatgc
    12601 tttatcagcc tcttaatcag gtgaataccc cacctttttt aatcatgttg gagaaattag
    12661 cagactttta gctcttatcc ctgtgtcaag tctactagaa gttctgcgat tatttcggtg
    12721 gaaaaagaag aaatcccata ggctttggct ataaatgtga tataatgaag tcactatatt
    12781 atttttttag gagttacatt aacatggaag accctgtgag tcagccctta gtgattcaat
    12841 ttttattgtt agttgtttta accttgttaa atgctttttt ttcagccagt gaaatggcct
    12901 tagtttctct caatcgttct cgggtggaac aaaaagcagc agacggtgat aaaaaatacg
    12961 ctcgtttgtt gcgggtttta gaggaaccta atcatttttt atcaacgatt caagttggga
    13021 ttacctttat tagtttacta tcaggagcaa gtttatcagc ttctttgggt aaggtgatct
    13081 caggttggct aggtaattca gcgaccgcaa ggacagctgg tactatcatc tccttggttt
    13141 tcttgactta tgtctctatt gttttaggag aattgtatcc aaaacggatt gccatgaacc
    13201 tcaaagacaa gttggcgatt gtttcagccc ctattatcat tgggttaggg agactggtta
    13261 gtccctttgt atggctctta tcagcttcta ctaatttact gagccgactt acccctatga
    13321 cctttgatga tgcagatgag caaatgacac gtgatgaaat cgagtatatg ttatcaaaaa
    13381 gtgaggcgac ccttgatgct gaagaaattg agatgttgca aggagttttc tcacttgatg
    13441 aaatgatggc gcgtgaagtc atggtcccaa ggaccgatgc tttcatgatt gacattaacg
    13501 atgatccgct tgaaaatatt caggaaatct taaaacaaag tttttcacgc attcctgttt
    13561 atgatgtgga taaagataaa attatcggtc tcatccacac taagcgtctc ttggagtcag
    13621 gtttccgcca gggatttgat cagattaaca tgcgaaaaat gttacaagaa cctctttttg
    13681 ttcccgaaac catttttgta gatgatctct tacgccagct gcgcaatacc caaaatcaga
    13741 tggctatttt gctagatgaa tatggtggtg tggcaggact tgtgactttg gaagacttgc
    13801 ttgaagaaat cgtcggtgaa atcgatgatg aaaccgataa agcagaacaa tttgttcatg
    13861 agattggaga caatacctat attgttgttg gtactatgac tttaaatgag tttaatgact
    13921 attttgatac cgaactagaa tcagatgatg tagataccat tgctggtttt tatttgacag
    13981 gtatcggaac cattccaagc caggagcaaa aagaagccta cgaaatagat aacaaagaca
    14041 aacatttagt tctaatcaac gataaagtca aagatggccg tattacgaaa ttaaaattaa
    14101 tcctgtctaa tatagaacag attattgagg aagactaggc tccatgccta gtctttttga
    14161 tgaaaagtgt tataatgaaa atgtaaaaac gtttacaagt aatgaaggag atgcaatgac
    14221 tgagaaagat tatggacaag taacagggat ggtgcattcg acagaaagtt ttggttctgt
    14281 agatggtcca ggcattcgtt ttatcatttt cttgcaaggc tgtaaattac gttgccaata
    14341 ttgccataat ccggatactt gggagatgga aaccaacaat tcaaaaatca gaacagtcaa
    14401 tgatgtcttg aaagaagccc ttcagtacaa acatttctgg ggaaaaaaag gaggaattac
    14461 cgtatctggt ggtgaagcta tgcttcaaat tgattttatc actgccttat tcatcgaagc
    14521 taaaaaatta ggtatccata ccacactaga cacctgtggc tttacctata gaccgacacc
    14581 tgaataccat caggttcttg ataacttatt ggcagtgacc gatttgattt tattggactt
    14641 aaaagaaatt gatgaaaaac aacataaaat tgtcacgcgt cagcctaata aaaacatatt
    14701 gcaatttgct aggtatttat ccgacaagca gattcctgtc tggattcgac atgtcttggt
    14761 tccaggcttg actgatattg atgatcattt gacacgattg ggtgagtttg tcaaaacctt
    14821 gaaaaatgtc gataaatttg aagttttgcc ttaccatacc atgggagaat tcaagtggcg
    14881 tgagctaggt attccttatc agttagaagg tgttaaacca ccaactaagg aacgcgttca
    14941 aaatgctaaa aatctgatgc agaccgaatc ttacacagaa tatatgaatc ggattcatca
    15001 gtcataagag ctgttatagg gttgaggttt cttcaacttt ttaaagtatt ataagcagat
    15061 cttttgtaat cttcctttga ttttaggggg taattttgct aaaataaaat gaataaacaa
    15121 aatgaggtag aatgattatg tcaaaaattt tagtttttgg tcatcaaaat cctgatacgg
    15181 atgctattgc atcatcttat gcttttgatt acttatccca aaaagcgttt ggtttggata
    15241 ctgaagtcgt agctttagga acacctaacg aagaaacagc ttttgccctt gattattttg
    15301 gtgttgaggc accacgtgtg gtagagtctg ctaaagcaca agggtctgaa caagtgattt
    15361 tgacagacca caatgaattc caacaatcca ttgcagatat ccgtgaggtt gaggtatatg
    15421 gtgtggttga tcaccaccgt gtggctaatt ttgaaactgc aaatccttta tacatgcgtg
    15481 tggaaccagt aggatcagcc tcatctattg tttaccgtat gtttaaagaa aatggcatcg
    15541 aggtgccaaa agcgattgca gggatgctct tgtctggttt gatttcagat accttgttgt
    15601 tgaaatctcc aacaactcat gtgtctgatc accttgtcgc tgaagaattg gcagagctag
    15661 cagaagtgaa cttggaagac tatggaatgg ctcttttaaa agcaggtaca aaccttgcaa
    15721 gcaaatcaga agtagaattg attggtattg atgccaaaac atttgaatta aatggcaatg
    15781 ctgtgcgcgt ggcacaagtg aacactgttg acattgcaga agtcttagaa cgtcaagaag
    15841 ctattgaagc agccattaaa gatgccatgg ctgcagaagg ttattcagat tttgttttga
    15901 tgatcacgga tattgttaac tcaaactctg aaattctagc tattggagca aacatggaca
    15961 aggttgaagc agcctttaac ttcacccttg acaacaatca tgccttctta gcaggtgccg
    16021 tttctcgtaa aaaacaagtg gttccacaat tgacagaaag ttttggagct taatgaaaag
    16081 acgctttaaa aggacttgat ttttttgacc aagtcctttt gtcactcttc tatgttataa
    16141 tagaaggtat gataacagta tttcattcag ataaactcac cagacaacct ttctttcaag
    16201 accttatcaa ttatctcgac cagcatgatc acgttatatt acgagagatc aaaaaagcct
    16261 ttcctaatgt gacaggtatt gacaaggcca tcgaaagcta tgttcaagct ggctatattc
    16321 gccgtgaaaa taagcgttat ggcatcaatc ttcctttggt gagttctgat cagcagctgg
    16381 ccttagacac tatgcttttt gtggacacct gttcagctat gtatgaaaat attttagcgg
    16441 ttgtttttga gactcagcta acaaaccaaa ccaatcgcgt gatgatcaag gaaaagacca
    16501 acatcacgag agacgatttg accctggcta attattttta ccgtctcaaa agaggtgaga
    16561 agccatcagc tgagcagatg gacttgtatg acctcttggg ggatgtgaat caggaatatg
    16621 cccttaaata tatgacaact tttttgctta aattcacgcg caaagacttt gtgatgcaaa
    16681 aacgtcctga tatatttgtg gaagctctgg ttacacttgg ttacctaaaa caagtggagc
    16741 ctacaacata ccagctgcta atgaccttgg ataaagaaag tttaaccttt atagcaccat
    16801 agaaaagagc taggaacaag gactgttctt agctttttga gtgcttttat tttgtttttg
    16861 ccattaagta aagaaaataa ggggctccga taagcgacac taaaatccct gtctggatat
    16921 tactgccaac cagatagact cgtcctactg tatctgcaac aagaagtagg atcattccga
    16981 ttaaaatact aatgggtatt gttattttat gattactccc taccaagtat gtactaaagt
    17041 gtccagctat taagccgata aaactaatat taccaaccaa aagaacactc aaagcaccta
    17101 agccagctgc taagacaagt atcaaacgac gcttacggtt taaagggagt cctagcccaa
    17161 tagcagtatt atcagctaat cccataatat taaggaaatg ggcttgacta taagtcaata
    17221 gccaaaaaca caataacaaa ggagcgatga cactcagagt aggccagtcg tctcctatca
    17281 attggccgct aagccaattg atgaccaaat cgactttgta gcgattaata tgaccaacaa
    17341 gagcaaccat tagacttgaa agcatggtag taacagctac accagtaaga attaaccgtg
    17401 taggatcaat ttgtccttgt tttgtcaaag atagccaata aacgctaaag gttgttacaa
    17461 tagcaccaag catggcaaat aaaggcaagt aatggctgat atgagcttgc tcaaagaaag
    17521 aaaaggagac cacaatagcc aaagagatac ctgctcctat agtaatacct aatacaccag
    17581 agtcagctaa gggatttcgt gttaaacgtt gtaataatag ccctgataaa gcaagagaac
    17641 cgcctcctag acaagcagct agaattcttg gcagacgaat gttaataacg atgaaagata
    17701 tggcatggct agattttccc aaaaacacat ggacaaggtc caaaaaagat agatgagatt
    17761 ctccaaggga caaagctaat cccataagac ttacaagtag caggatcagg ctagtatata
    17821 accttaaaag gtgtctttga gatttcaata gcggcctcct cttcttatta accataaaaa
    17881 gcatggaaaa ccaattaagc taaccaatgc cccaagaggg gtttcataag gcggattgag
    17941 attacgacaa gcaatatcaa cccataccat gaaactagcg ccagaaactg cacaaagcgg
    18001 caaaaggtat cggtaatgat gaggtgtaaa atgtttcata agatgaggaa tgactaagcc
    18061 gataaaagaa atgcttccag caatggctac agctgctgat gataaaataa gaaccaaaat
    18121 cataaagact gcactgatca agttggtttt ttgcccaaga gcttttgcct gagactcact
    18181 tagactaagg acggtcaggt gataagataa tagctgggct aaacaaagac taagaataat
    18241 aagaggagca atatagccga tcatttgcca attgaccccg acaagacctc ctgcttgcca
    18301 gccgataaca gcatttgcca gatgatagta attggtaata ccttgaccta atgctgatag
    18361 cagtatggaa accatagctc ctgctaagac aaggcggagc tgatggtagc ctttgccaga
    18421 ttgataagaa aggccaaaga ctaaggttgc tgccaggcta gaccctaaca acgaaagcag
    18481 aataatgagg gaatagtgca agtgaggtac aaacgcataa gccagtacca aggctagtcc
    18541 tgctccagca ttaatgccta aaaggccagg ctcagcgata ggattacgtg tgattgcttg
    18601 catgatagtt ccagaaacag ctaacgcaga gccagttaag gtagcaccaa aaagtctcgg
    18661 taatcgtata gcgagtaaaa cattggcttt atggccgttt tgctttccaa ataaaataga
    18721 gttaagatcc tgatgtgata aagcaacggc tccaaagcgc agatgacagt aaatagcaac
    18781 acataagcta aaactaagta gcacaaagac aagccaaaag atatgtgact tagtgattat
    18841 ctgcgttttt tttaagcaaa aacgctcgag acacaaaaag ggatgcttag ttttcacttg
    18901 ataagattgc ctctcgtaaa gtctctaatt gatattctaa agaaagagga tcagtaaagt
    18961 aaaaaacatt ggcattgact ttgatcacat ggtgtttttt cacagctgga atggattgcc
    19021 atagcttgct ttcatataga gcagaaccag tcttatcatc ttctgcagca acgacgacat
    19081 aatcaccaat atagtctggc aagacttcct gcgacaatga caggtaacct tgtttaaaaa
    19141 cttcagtctt cactttttca ggagcatcat aatggaaagc ttgatggata atctctccgc
    19201 cacgtcccca gtctttacca aaaaggtaaa cgtctttttc ataaagcccc ataatagtaa
    19261 aggttgcttt gtcacctgta acggccttga cttctttttc ataagctgct gttttagttt
    19321 tccaatcttt taaccacttc ttggctttgt cttctttgtt aaagatgcgg ccgaagtcag
    19381 ataacacttg taaataatca cgtttgcggt attcaatcga gataacaggc gcaatttctg
    19441 ccaattgttt aatattttct tctgtagagc caacaacaat gaggtcaggc ttaagcgttg
    19501 taacggcttc taaatcagtt gcagcaactt gctttgcttt ttttacagtc tttgctaaga
    19561 tggggttttt cttgtcataa gaagtaaccc cgactagatt catatctaat tttttgaggt
    19621 aaccagtgta agttgaagcc agactgacta ctcgcttagg ctgtttagga atatctccgt
    19681 aataagtcac accagcaatt tgaggcatag gcgataaact ttttgaggcg gtgttagaat
    19741 gattagttgc ttgattacca caggcgatta aggtaatagt tgtcaaacag agtgttaata
    19801 gtagagttaa ttttttcatg tatgctaatt ctcctagaat aaagttattg aagctgataa
    19861 gtcagcacta taggacagtt gtggatagga tcatcaacca gtaccggttt gatttggaag
    19921 atgtcttgaa tgataggtga agtcataaca tctgctatag taccagtata atggatcttg
    19981 ccatgtttca tagcaatcag atgatccgaa tagcgggctg aaagattgag gtcgtgaaga
    20041 accataacaa tagtcttgcc ggcatcctta tttagggatt taacgagctc taggatttct
    20101 agttgatgat ttaaatctaa ataagtagtt ggctcatcta aaaaaatagt atcagtgcct
    20161 tgggctaatg ccattgcaag ccaaaccctt tgacgttgcc ccccagaaag agcatcaaca
    20221 ggttgatcag cataggccat gacattggtg gcttgcatag cccaatgaat ggcttttttg
    20281 tcagctggac taagatttcc aaagtaagat tgatggggaa aacgtccgta agaaaccaat
    20341 tcgtaaacag ttatgccgtt agttgcttct tgaacttgag gtaaaagagc tagtttttta
    20401 gcaacctctt tagtttctaa tgtggcaatg ttttgcccat ttaaatatac aactccctgt
    20461 tttggtggta ataatcttgt cagtgctttt aataaactag acttcccaca gccattggcg
    20521 ccaataatgg tcgtaatttt accttcagga atataaaacg ataatttatc gatgatggta
    20581 cgctgttcat aggcaattgt gaggtcttca gcactaattg ttgtcatatg gttgcctcct
    20641 ttctaaaaag agtcaaaggg ttgatgaaag tgcacattaa agtgttcatc tgcccatttt
    20701 aaaatagttg agaagtagct tattttctca aaatcttcac tattatagca tagaaaaaag
    20761 gcttaacaaa gccttttttg atgtgatttt taggatgtat taaaaaagaa atattagttt
    20821 agaatgatta aaaacaatag cgataatcat tctaaactaa cgtcaacata ctctagaggt
    20881 agtgtttggc gattgctaaa tcaccagcat aagctacttg ttgtccttta acaatttgat
    20941 aagcatctgc gcctttccca gcgataatca cagcatcttt tgctccttgg caaagagaca
    21001 tggctttttg aatagcttgc tcacggtcac taataatctc tactgggcgt gcgatatggc
    21061 tggcaatttc ttttgaaata tcttctggat cctcgaaatt agggtcatca gcagtgagaa
    21121 tgacggtgag gtttggatgc tgatgaatca ccctaccaaa atcagctcgt ctgctctcgc
    21181 ctttatttcc tggtgcacct aaaatcaaca ttaatttgcc tgtctgatgt tcttccacaa
    21241 cgctgagtaa tttttctaag ctgtcaccat tatgggcata atcaacaaaa actttagcat
    21301 gatttgtcat agtgaggact tccatacggc ctgggacgcg agtcttagcg ataccttttt
    21361 gaatatcagc taggctagca cccaaacgaa ggcaggcaag tccagctgcc atagcatttt
    21421 cttggttaaa atggccaatt aattgaatgt cataatggcc agctaattgt cctttagctt
    21481 caaaggagaa ggcttggcta gtggtgatct ggttgtcaga caagggacca taaaacacat
    21541 gctcttgatc cgctacctga tccgctagaa atgaaaaatg atccatcccg ctattgatca
    21601 caacagctct gctattctcc attaaaagac gcttatgata gaaataatct tcaaacgttg
    21661 gatgctcgat tgggccaata tgatcaggac taatatttag gaaaacccca acatcgaacg
    21721 taagcccata gacacggtca acgaggtaag cctgactaga aacttccata atcaagtggg
    21781 tcatgccatt agtcacacac tcagccatca tagcaaaaag atccaagctc tcaggagttg
    21841 tgagttgtga tttgaaaaag gtcttgccat ctaaggtggt attcatggta gaaaacatgg
    21901 caggtttata ggattctttc aacatgtggt aggcaaaata agcagccgtc gtctttccct
    21961 tagtacctgt aaaagctagt agtttgagct tttcttgagg gttgccatag aaagccatgg
    22021 caattagact catagctttt ttgatatcag tgaccaaaac aacgggaata ccaagctcgt
    22081 aatctacctc tgaaatatag agttgtaatc cgttagtgat tgcttcttta aggtagtcag
    22141 ctttgaaagt tgccccttta gcaaaaaaaa gggtcttgcc atctacttga cggctatcgt
    22201 aactcaagcg ctcaaaacta aagccttgat aatggtagtg gtatccgtcg gcatcaagaa
    22261 cctcacgaaa attatggtct ttttttaaaa tgtctaataa ttgttcaatg gttatcatac
    22321 ccttatttta tccttaaagt tcttgtttta caagtcacaa gcaaaagttt ataataaagc
    22381 taatagaaac taggaaagaa tagatatgtc gacagaaaaa aaacaactga cacaagaaga
    22441 attgatggtt cagggagcag cctggtccac agctggtaat ttcattagcc gacttttggg
    22501 agtgttatac attattccct ggtacatttg gatggggcaa tacgcgattc aagccaacgc
    22561 tcttttcaat atggggtata atgtttatgc ttatttttta ttgatttcaa ccacaggact
    22621 caatgttgcg attgctaagc aggttgccaa gtacaattcc atgggacaaa ctgagcatag
    22681 ctatcaatta atccgaagta ccctaaaact catgcttggt ctgggcctta ttttttcagc
    22741 cattatgtat ctgggctctc ctttatttgc gagcttatct ggtggtgatg acacacttgt
    22801 tcccattatg cacagccttt ctttagcagt ttttattttt cctgtaatga gtgtgattcg
    22861 aggcatcttt caaggccaca ataatatcaa gccttatgca gttagtcaga ttgctgagca
    22921 actcattcgg gttatctgga tgcttttgac cacctttttt atcatgaagt tgggttctgg
    22981 tgattacgct tcagcagtga ctcagtcaac ttttgcagcc tttatcggga tggtggctag
    23041 tatgggtgtt ctggggtact atctttggaa acagggactt ctagcagcta tttttagtaa
    23101 gccagatcat actgtttcta ttgatatcaa gggcttgttg cttgaaacct taaaagaatc
    23161 catccccttt atcgtaacag gaagtgccat tcaagccttt caattgattg atcaatggac
    23221 ttttgtcaat acgatgactc tttttacaga ttatagccgt tctcaattgc ttgttttatt
    23281 tggttacttt aacgcaaacc cagccaaaat tacaatggtc ttaattgcag tagcagcatc
    23341 tattggtggt gtaggtattg ccttgttaac tgaaaattat gtcaaaaaag acatgaaagc
    23401 agctgctcgt ttaatcatta acaacattga aatgttagtg atgtttttgt tacctgctct
    23461 tactggggca attattttag caagacctct atattctgtt ttttacggag ctagcgagga
    23521 gcgtgccatt cacctctttg tggcggttct ctttcaaacc ttgctactgg cgctttacac
    23581 cctcttttca ccgatgcttc aagctctttt tgaaaatcga aaagcgattt actactttgc
    23641 ctatggtatc ttgattaagt tagttttaca gataccgctt atttatttgc tacatgctta
    23701 tggtccttta ctagcgacga cgattgcttt agtggtgccg atttatttga tgtatcgacg
    23761 cctatatcag gttactcatt ttaaccgcaa actgttgcaa aaacgtttat tattaacctt
    23821 aattgaaacc ttattaatgg gactggtcgt gtttgtggcc aactggctat tgggctatgc
    23881 ctttaaaccg acaggccgct tgaccagcct tctttacctc ctcattattg gtggcttggg
    23941 aatgacggtt tacacggcac tcaccttgct gacgcatcaa ctggataaat taattggtag
    24001 caaagctagt cgccttcgtc agaaattagg ctggcattaa tataatgaca ccaagaacga
    24061 tttcgtgagt gcttatcgaa atcgtttttt tcttaatgat ctcgcttagt aaaggtagct
    24121 gataaaaaat caggatttcc caagcaaact actgatattt ttggtataat gataaaagtg
    24181 taaaaaaata gaaggaagta tatcaacatg ggaaaatgtc aagttatttc gcatccgctt
    24241 atccaacaca aactttcaat cttacgtcgt caaaccacat caactaaaga ttttcgtgag
    24301 ttggttaatg aaattgcgat gctgatggga tatgaagtgt cacgcgacct tcccttagaa
    24361 gatgtcgaca tccaaacgcc agtctcaaaa acggtccaaa aacagttagc tggtaaaaaa
    24421 ttagctattg ttccgattct tcgtgctggt attgggatgg ttgatggctt gttaagtttg
    24481 gtaccagctg caaaagttgg ccatatcggc atgtaccgta atgaagaaac ccttgaacct
    24541 gttgaatacc tcgttaaact accagaagat attaaccaac gacaaatttt ccttgttgat
    24601 ccaatgttgg caacaggtgg ctcggctatc ttagcagtgg attcgttgaa aaaacgtggc
    24661 gcagcaaata ttaagtttgt ctgtttagtt gcagcaccag aaggggtgaa aaaacttcaa
    24721 gaagctcatc cagatattga tatcttcaca gcagcacttg atgatcattt aaatgaacat
    24781 ggctatatcg tgccaggatt gggcgatgct ggtgatcgtt tatttgggac aaaataaaag
    24841 agacaaccat caaatagtag cctttttagg ccaatcgttc ttgattttgt ttaaaaaatc
    24901 agagagattt ctcttcttta aaggaaaagc aagtttatga tttgaccttt attgaccaaa
    24961 atattataat aaagacaatc agtaaaaaag agatgaccta gtgggttgcc gtctctcaaa
    25021 atgacgctac taaaaaccct tgtagattgg agaatgtatt atgattcctg ttgttattga
    25081 acaaactagc cgtggagaac gttcttatga catttattca cgtttattaa aagaccgtat
    25141 tatcatgtta acagggcctg tcgaagacaa catggccaac tctgtcatag cgcagctctt
    25201 attccttgac gcgcaagaca acactaaaga tatctaccta tatgtcaaca caccaggtgg
    25261 ctctgtctca gcaggcttgg ctattgtcga taccatgaac tttatcaagg ccgatgtgca
    25321 aactattgtc atgggtatgg cagcttctat ggggacagtc attgcttcat caggaacaaa
    25381 aggcaaacgc tttatgttac caaatgcaga gtatatgatc caccagccaa tgggtggtac
    25441 aggtggtggc acgcaacaaa cggatatggc tatcgcagca gaacatctct taaaaacacg
    25501 tcaccgttta gaaaaaatct tagcgcaaaa tgctggaaaa acgattaaac aaatccataa
    25561 agatgctgag cgtgattatt ggatgagtgc tgaagaaacc ttggcttacg gtttcattga
    25621 tgaaatcatg gaaaacaacg aactaaaata aagaggatct ctgcaaaagt attctagaaa
    25681 cttctaggat gctttttctc ttagaaagga aaataatgat acagcctact gcattaaaac
    25741 agcttcaaga gttaaaacgt cagttcagat ccctttgcaa ccaattatga gatagtttga
    25801 ggatgatcct tagcagctgt gtgcaagaag ttatgacgat ttcataaagg atgacctagc
    25861 cagtctttct tcgtctcttt tacttaaagt gtggtataat cagattatgc tagccaaaac
    25921 taaaaaaggc attgccatta aaggtgttgt agatcggctt atcctagatc tcataacaat
    25981 gctgtagtac cacccttacc ttgctactaa aggcgctgtt gaagcacaaa ttgacttgca
    26041 aaggatcttg actgataata tgtccggtgg aatcgccaaa gcagcaaaaa gaccaagacc
    26101 cttatttggt ctctaccaaa atataataga aaaggattta gaatgtttca aaaacaagaa
    26161 cgtattggac tcgtcgttta cctgtattat aaccgagatg ccagaaagtt atcaaaattt
    26221 ggtgatttat attaccattc caagcgctct cgttacctca ttatttatat taataaaaat
    26281 gatttagaca caaaattaga agaaatgaga cgtttgaaat gtgttaaaga cattagacca
    26341 tcagcttttg atgatattga ccgccaattt gtaggtaacc ttcatcgcga cgaaacaaac
    26401 aaccatcaaa agggttaaca accccctagt tactgattag cgtttcagat ttagttagct
    26461 tattaaagta agaaggtttt gacagatgct cacactactt caagacaatg agacaactag
    26521 agcattagtg aataactgag ttgactgtca cttctttaat aagaacggat aggattggtc
    26581 ctaaaataga gcactgcaaa gatatgccag tgctttttag ctcatttttt gataaaatag
    26641 aactactaaa aagtgaagga aataagatga tcacaggaaa actcataacc gttgaaggtc
    26701 cagatggcgc agggaaaacg actgttttag agcaactcat tccactgtta aaacaaaaag
    26761 tggctcagga tatcttgaca acaagagagc ctggaggtgt tgccatttca gaacacatta
    26821 gagaacttat tttagacatc aaccatactg ctatggaccc taaaacagaa ctcttacttt
    26881 atattgcggc tcgaaggcag catttggtgg aaaaggtact accagctctt gaagcaggcc
    26941 agttggtttt tattgatcgt ttcatcgata gttcagtagc ttatcaaggt gcgggacgcg
    27001 gtttgatcaa agcagacatt cagtggctca acgagttcgc aacagatggt ttagagcctg
    27061 acttgaccct ttattttgat gttccttctg aaattggttt agcacggatt aacgcgaacc
    27121 aacaaagaga agtcaatcgt ttagatttgg aaacaataga gatacatcaa cgagtacgaa
    27181 aaggatacct agccttagct aaggagcatc caaaacgtat tgtcacaatt gatgcgacta
    27241 aaccattgaa agaagttgtc tctgtcgctt tggaacatgt cttagccttg cttttagcct
    27301 aggataaggg agggtaggaa tggatttagc gcaaaaagct cctaacgttt atcaagcttt
    27361 tcagacaatt ttaaagaaag accgtctgaa tcatgcttat cttttttcgg gtgattttgc
    27421 taatgaagaa atggctcttt ttttagctaa ggtcatcttt tgtgaacaga aaaaggatca
    27481 gacgccctgc gggcattgtc gctcttgtca attgattgaa caaggagatt ttgccgatgt
    27541 gacggtattg gaaccaacag ggcaagtgat taaaacggat gtggtcaaag aaatgatggc
    27601 taacttttct cagacaggat atgaaaacaa acgacaagtt tttattatca aagattgtga
    27661 caaaatgcat atcaatgccg ctaatagctt gctaaaatac attgaggagc ctcagggaga
    27721 agcttacata tttttattga ccaatgatga taacaaagtg cttccgacca ttaaaagtcg
    27781 gacacaggtt tttcagtttc ctaaaaacga agcctatctt taccaattgg cacaagaaaa
    27841 gggattatta aaccatcagg ctaagctagt agccaaactt gccacaaaca ccagtcatct
    27901 agaacgtctg ttgcaaacga gcaagctttt agaactgata actcaagcag agcgttttgt
    27961 atctatttgg ctgaaagatc agttgcaggc atatttagcg ttgaaccgtc tggtacagtt
    28021 agcaactgaa aaagaagaac aagatttagt tttgaccctt ttgaccttgc tcttggcaag
    28081 agagcgtgcg caaacgcctt tgacacaatt ggaggctgtc tatcaggcta ggctcatgtg
    28141 gcagagcaat gttaattttc aaaacacatt agaatatatg gtgatgtcag aataagtgca
    28201 attggaattc gttatgggga aacgaagctt gtcaaaagac ttgcccgtag cgaccaaagt
    28261 agcggcgcat aagggcaaaa aagcttttga agaaaatact gcctttgcaa cagatttgat
    28321 gaaagaagta tcacgattaa taacacaaaa tcaacttgaa atgacgtttg ttaatagtgt
    28381 ctttctctta gatcagcatg ttgtcctaat tcccttttca gcagaagagc acatggattt
    28441 ttacaactgt taaaagattt agcggtcgat tttaggtcaa gaagtgagct gccccagatt
    28501 aacagccaag aagatttcat tttctatgga ggctatggac cgtgtggcag gccactgtgc
    28561 tgctttcgtt tttgggaaaa tttctgacag tatctattaa aatactaaaa agtcaagggt
    28621 tatcattgac tttgggtaaa gcaacaagcc cttatggccg tttgctttgc tgtctctatg
    28681 atgaaggtgt cttttaccaa taaagcaaga aaaaattccc tgaatatggt accatagtag
    28741 agacaagtga tggccctgca acagtagcag ccgttgatat atttacagat cccattaagg
    28801 tgcggttaaa agaccagttg accttagtca cttatgcttt agaggaggtt aaagtaggtg
    28861 aataaaaaag aattattcga tgcttttgat ggattttccc aaaatttaat ggttacttta
    28921 gctgaaattg aagccatgaa aaaacaagtc caatcactag tagaagaaaa cacgattctt
    28981 cgcctagaaa atacaaaact acgggaacgt ttgagtcacc tagaacatga aacggtagct
    29041 aaaaatcctt ccaaacaaag gaaggatcac ttagagggaa tttatgatga agggtttcat
    29101 atctgcaatt ttttctatgg acaacgtcgt gaaaatgatg aagaatgtat gttttgcaga
    29161 gaattattag atagaaagta agggcatgca ggttcaaaaa agttttaaag acaaaaaaac
    29221 aagcggaacc ttgtatttgg ttccaacccc aattggcaat ctgcaggaca tgacctttag
    29281 agcagtggca acgttaaaag aggttgattt tatttgtgca gaagacacaa gaaacacagg
    29341 tcttttgcta aaacactttg atattgctac caagcaaatc agctttcatg agcacaacgc
    29401 ctatgagaag attcctgatt taattgattt attaatatca ggacggtcac tagctcaggt
    29461 gtctgatgca ggaatgccat cgatttctga tcctgggcat gatttggtca aggctgctat
    29521 tgatagtgat atcgcagtcg ttgctttacc aggtgcctca gcagggatta cagctttgat
    29581 tgctagtgga ctggctcctc aaccccatgt tttttatggg tttttaccca gaaaagcagg
    29641 gcaacaaaag gctttctttg aggacaaaca ccattaccca gagacacaaa tgttttatga
    29701 atcaccctat cgcatcaaag ataccctgac caatatgttg gcttgttatg gtgatcgtca
    29761 agtggtgctt gtcagagaat taaccaagct ttttgaggag taccaacggg ggtcgatttc
    29821 agaaatcttg tcctaccttg aggaaacacc cctcaaaggg gagtgtctgc tgattgtggc
    29881 tggagcccaa gcagacagtg aggttgagct gacagctgat gttgacttag tgtctctagt
    29941 gcaaaaagaa attcaagcag gagccaagcc aaatcaggct attaaaacaa ttgctaaagc
    30001 ttaccaggta aatcgccaag agctttacca acaatttcat gacttataag tcatcgtaga
    30061 ttaggagaaa aacaaatgac agaagatcaa gcaactatcc ttattttagg agtatacgga
    30121 atcatattgg ctgtattctt attatcagct ttattatgcc ttatcgctaa ctggcgactc
    30181 tttaaaagag caggttacaa aggatggtat agtctcattc ctttctatgc aggttttgtc
    30241 aaacacaaaa tcacttttgg agaagataat aaatggtttt actttattgg ttgggtcatt
    30301 gctatttatt attactatgt gcacttttgc tatgtccgtg cttttggtgg atcaaagggt
    30361 ctagcagtat ttggcttgtt tttcccaggc attgcttctt tgatagtggc ttttggaaaa
    30421 acttatacca cacaagaatg ccaccctgtg ccacatcttt taaactaaga gaaaaaagac
    30481 catcaattgg atttattgat ggtcttttag gtgtttaaat gattttagtc ccgtgaacaa
    30541 tggccgttcc agtgagttga ctcaagcttt ggcaattctc agcagtgatg cctcccccaa
    30601 tcatgatttc aatgcgttta ttggcatagg tcacaagact tttaagctgt tccacattgt
    30661 ctgtaatagg agtagcttca ggtgagccat gagttagcac acgcacaaag ccataatcaa
    30721 tcagttggtc aagcgcttgg tgctggtgat cagtcggaat ccggtcaaaa gccatgtgga
    30781 aaaccagtgg caagccttgt gtagcaggca gtaattgctc gatagcgtcg gtatccagtt
    30841 gattttctgt ggtcagcaag cctaagacta gcgcatctga gccagcttca acagctttta
    30901 agatatcttc ttccatagct tttagttcga ggtcgttata gacaaaatcg ccaccgcgag
    30961 gtcgaatcat ggtcgctacg ctaatctttt tatcgtgtaa gagctgacag gcttctttga
    31021 tgacgccata agatggcgtg gtccctccga cagctaggtt atcacagagt tccacacgac
    31081 tgatttgacc agcatcaagg gttggcagta gtgtgaggtt ctcagcacaa aattctttaa
    31141 tcatttttag atctccttta tcagtaatat tatagcacga atgaagctag tcatcaggaa
    31201 acatttttgt caaatcatgg acattatgct atactcacta gtattaaaag taggccaaaa
    31261 cagcagcaaa tactttgaaa aaagattgct atgttctacg gcaatatcag tctttaccaa
    31321 cgaactctca agaagaaaag aaagacaaag cttagcattt taaaaaagcc cccttttttt
    31381 tgtgaaatga tgaccaaaaa caggcgcctc acgcctaaaa cagttacact aaaggagaac
    31441 aaatgattac gatttaccaa tacccaaaat gtagcacctg ccaaagagcc atggctgaat
    31501 taaagcaact ggttagtgac tttgaagcca ttgacatcaa agctaatcca cccaaagctc
    31561 aggacctcaa acattggatg gaaacatctg gttataccat caaaaatttc tttaatacca
    31621 gtggtaacag ctaccgcgaa ttgggactta aagataaaat agaccagctt agccttgata
    31681 aagctgctga acttttagcc acagatggta tgttgattaa acggcctatt ctgattaagg
    31741 acggtaacgt cttacaagtt ggctatcgca agccatatca agagttggat ttaggataat
    31801 gcgacaaagt ctcaaaaaag gacaaggcaa cattataacg ccttgtcctt ttttgggatc
    31861 tgtttttaga aattagagat caatatctag taaaattggc gtatggtctt ggcgttcccc
    31921 agaagaaatc atttcagaac gcttaacctt atccacaagt cggtttgaag ctagccaata
    31981 gtcaattctc caaccggtat tattgatttt gctggttttg ctacgttgtg cccaccaggt
    32041 ataaacatga ggaatatcgc cgtgcacgtg acgaaaagta tctgtaaagc ctcttgctaa
    32101 taggttagta aagccttgac gttcttcgtc ggtaaaacca ggagaacgtc gattgctgtt
    32161 tggatttgcc aaatcaatct ctttatgggc aacattgtaa tctcccgtag ccaaaacagg
    32221 cttttgagca tccagttcag tcaaataatc agcgtatttg tggtcccaaa tttgacggtc
    32281 atctaaacgt ctaaggccat caccagcatt aggcgtgtaa acctgtgtga cgaaaaagtc
    32341 ttcaaattct agggtaatga tacggccctc agcatccatt gttgttgggg caccaatttc
    32401 tggaaaagta atgacagggt ttaacgtgtt tttgtacagg aacatggtgc cagcatagcc
    32461 tttcctagca ggctcaactg aagaacgcca cacatgcaaa tagtttggaa agtaactaag
    32521 tagggtctcg atatgttttt tggtaggccc cttagcagat aatttagttt cttggatggc
    32581 aataatatcc gcatcttgag cgactaaagt gtctaaaaca gcgcgtgata aaagcgcacg
    32641 aggggactct cctgtgagag cagcatttaa ggaatcaatg ttccatgaaa tcagtttcat
    32701 ttggtttctc tttctaacag atagtcttgt tgttattgta acaaaaaacg aaacgataag
    32761 cgactttggg cacctagccc ccataaaaca gtctagccaa atgaagcata gcagcgtaag
    32821 aacaagacaa ggaggtgatc agtgagcata tcgtttgaca gttaagagta ggattactat
    32881 aatagaagca taaatgtaag cgctaacaaa ataaaaatat aggagaatgt caatggcaca
    32941 aaaaacagtc atcaccgaag aaacaaccga ttttgttatg gatttcaaaa caagtagcgc
    33001 tgagggaaat gttgatttta ttaacgtttt tgaccttgag aaaatggcac aacaggtcat
    33061 ccctaaagga gcctttggtt atattgctag tggtgctggt gataccttta ccctccatga
    33121 aaatattcgc tcatttaatc ataaattaat tgtccctcac tccctcaaag gggttgaaaa
    33181 tccgtcaaca gagattacgt ttgatggtga ttacttaacc tctcctctta ttttagcacc
    33241 tgtagcggct cataaactgg ccaatgaaca aggagaagta gcgtctgcta aaggattgaa
    33301 ggaatttgga agtatctaca ccactagttc ctactcaaca actgacctgc cagaaatcag
    33361 tgctgcctta ggtgggacac cccattggtt tcaattttat tacagtaaag acgatggcat
    33421 taatcgtaat attatggatc gtgtcaaagc acaaggttgc aaagctatcg ttttgacagc
    33481 agatgcaaca gttggaggaa accgcgaagt tgataggcga aacggttttg tttttccggt
    33541 cggtatgcca attgttcaag aatatttacc tgatggtgca ggtaaaacaa tggattatgt
    33601 ctataaatca gctaaacaag cgcttacctc aaaagatatt gaatatatcg caacttactc
    33661 aggcctgcct gtttatgtca aaggaccaca gtgtgctgag gataccttgc gggcattgga
    33721 cgcaggagca tcaggtatct gggtaaccaa tcatggtggc cgtcaattag atggtggtcc
    33781 agctgctttt gattccttac aagaagtcgc tgaagcagtt gatcaaaaag ttcctattgt
    33841 ctttgactca ggtataagac ggggtcaaca tattttcaaa gctcttgctt caggtgctga
    33901 ccttgttgcc ttggggcgtc cagcgattta cggcttggct atgggaggaa gcattggtac
    33961 tcgtcaggtc tttgaaaagt taaatgacga gttaaaaatg gttatgcaac tggctgggac
    34021 acaaactatc caagatgtta aagcctttaa ccttcgccac aatccgtatg attcatcgat
    34081 tccatttgat caaaatgcgc tacgcttaga ttagaacaga caaaaacctc cgctatcatg
    34141 tcacaaatag tagaggtttt acactgcaaa aagaggtatg aaaatccaat aacatagtta
    34201 caaggttttc gtttaccccc cactaataga ttaaaatgaa agacttctta cattaataga
    34261 aatttctgtt gcttttctca gaaaaatgtg gttaaataat caaatattat taacgaaaaa
    34321 tgagttaatg attaacatta aactggtttt agttaaaaca tttaggaggg tatcaggtgg
    34381 agaaaaagca acgtttttcc cttagaaaat acaaatcagg aacgttttcg gtcttaatag
    34441 gaagcgtttt cttggtgatg acaacaacag tagcagcaga tgagctaagc acaatgagcg
    34501 aaccaacaat cacgaatcac gctcaacaac aagcgcaaca tctcaccaat acagagttga
    34561 gctcagctga atcaaaatct caagacacat cacaaatcac tctcaagaca aatcgtgaaa
    34621 aagagcaatc acaagatcta gtctctgagc caaccacaac tgagctagct gacacagatg
    34681 cagcatcaat ggctaataca ggttctgatg cgactcaaaa aagcgcttct ttaccgccag
    34741 tcaatacaga tgttcacgat tgggtaaaaa ccaaaggagc ttgggacaag ggatacaaag
    34801 gacaaggcaa ggttgtcgca gttattgaca cagggatcga tccggcccat caaagcatgc
    34861 gcatcagtga tgtatcaact gctaaagtaa aatcaaaaga agacatgcta gcacgccaaa
    34921 aagccgccgg tattaattat gggagttgga taaatgataa agttgttttt gcacataatt
    34981 atgtggaaaa tagcgataat atcaaagaaa atcaattcga ggattttgat gaggactggg
    35041 aaaactttga gtttgatgca gaggcagagc caaaagccat caaaaaacac aagatctatc
    35101 gtccccaatc aacccaggca ccgaaagaaa ctgttatcaa aacagaagaa acagatggtt
    35161 cacatgatat tgactggaca caaacagacg atgacaccaa atacgagtca cacggtatgc
    35221 atgtgacagg tattgtagcc ggtaatagca aagaagccgc tgctactgga gaacgctttt
    35281 taggaattgc accagaggcc caagtcatgt tcatgcgtgt ttttgccaac gacatcatgg
    35341 gatcagctga atcactcttt atcaaagcta tcgaagatgc cgtggcttta ggagcagatg
    35401 tgatcaacct gagtcttgga accgctaatg gggcacagct tagtggcagc aagcctctaa
    35461 tggaagcaat tgaaaaagct aaaaaagccg gtgtatcagt tgttgtagca gcaggaaatg
    35521 agcgcgtcta tggatctgac catgatgatc cattggcgac aaatccagac tatggtttgg
    35581 tcggttctcc ctcaacaggt cgaacaccaa catcagtggc agctataaac agtaagtggg
    35641 tgattcaacg tctaatgacg gtcaaagaat tagaaaaccg tgccgattta aaccatggta
    35701 aagccatcta ttcagagtct gtcgacttta aagacataaa agatagccta ggttatgata
    35761 aatcgcatca atttgcttat gtcaaagagt caactgatgc gggttataac gcacaagacg
    35821 ttaaaggtaa aattgcttta attgaacgtg atcccaataa aacctatgac gaaatgattg
    35881 ctttggctaa gaaacatgga gctctgggag tacttatttt taataacaag cctggtcaat
    35941 caaaccgctc aatgcgtcta acagctaatg ggatggggat accatctgct ttcatatcgc
    36001 acgaatttgg taaggccatg tcccaattaa atggcaatgg tacaggaagt ttagagtttg
    36061 acagtgtggt ctcaaaagca ccgagtcaaa aaggcaatga aatgaatcat ttttcaaatt
    36121 ggggcctaac ttctgatggc tatttaaaac ctgacattac tgcaccaggt ggcgatatct
    36181 attctaccta taacgataac cactatggta gccaaacagg aacaagtatg gcctctcctc
    36241 agattgctgg cgccagcctt ttggtcaaac aatacctaga aaagactcag ccaaacttgc
    36301 caaaagaaaa aattgctgat atcgttaaga acctattgat gagcaatgct caaattcatg
    36361 ttaatccaga gacaaaaacg accacctcac cgcgtcagca aggggcagga ttacttaata
    36421 ttgacggagc tgtcactagc ggcctttatg tgacaggaaa agacaactat ggcagtatat
    36481 cattaggcaa catcacagat acgatgacgt ttgatgtgac tgttcacaac ctaagcaata
    36541 aagacaaaac attacgttat gacacagaac tgctaacaga tcatgtagac ccacaaaagg
    36601 gccgcttcac tttgacttct cactccttaa aaacgtacca aggaggagaa gttacagtcc
    36661 cagccaatgg aaaagtgact gtaagggtta ccatggatgt ctcacagttc acaaaagagc
    36721 taacaaaaca gatgccaaat ggttactatc tagaaggttt tgtccgcttt agagatagtc
    36781 aagatgacca actaaataga gtaaacattc cttttgttgg ttttaaaggg caatttgaaa
    36841 acttagcagt tgcagaagag tccatttaca gattaaaatc tcaaggcaaa actggttttt
    36901 actttgatga atcaggtcca aaagacgata tctatgtcgg taaacacttt acaggacttg
    36961 tcactcttgg ttcagagacc aatgtgtcaa ccaaaacgat ttctgacaat ggtctacaca
    37021 cacttggcac ctttaaaaat gcagatggca aatttatctt agaaaaaaat gcccaaggaa
    37081 accctgtctt agccatttct ccaaatggtg acaacaacca agattttgca gccttcaaag
    37141 gtgttttctt gagaaaatat caaggcttaa aagcaagtgt ctaccatgct agtgacaagg
    37201 aacacaaaaa tccactgtgg gtcagcccag aaagctttaa aggagataaa aactttaata
    37261 gtgacattag atttgcaaaa tcaacgaccc tgttaggcac agcattttct ggaaaatcgt
    37321 taacaggagc tgaattacca gatgggcatt atcattatgt ggtgtcttat tacccagatg
    37381 tggtcggtgc caaacgtcaa gaaatgacat ttgacatgat tttagaccga caaaaaccgg
    37441 tactatcaca agcaacattt gatcctgaaa caaaccgatt caaaccagaa cccctaaaag
    37501 accgtggatt agctggtgtt cgcaaagaca gtgcctttta tctagaaaga aaagacaaca
    37561 agccttatac agttacgata aacgatagct acaaatatgt ctcagtagaa gacaataaaa
    37621 catttgtgga gcgacaagct gatggcagct ttatcttgcc gcttgataaa gcaaaattag
    37681 gggatttcta ttacatggtc gaggattttg cagggaacgt ggccatcgct aagttaggag
    37741 atcacttacc acaaacatta ggtaaaacac caattaaact taagcttaca gacggtaatt
    37801 atcagaccaa agaaacgctt aaagataatc ttgaaatgac acagtctgac acaggtctag
    37861 tcacaaatca agcccagcta gcagtggtgc accgcaatca gccgcaaagc cagctaacaa
    37921 agatgaatca ggatttcttt atctcaccaa acgaagatgg gaataaagac tttgtggcct
    37981 ttaaaggctt gaaaaataac gtgtataatg acttaacggt taacgtatac gctaaagatg
    38041 accaccaaaa acaaacccct atctggtcta gtcaagcagg cgctagtgta tccgctattg
    38101 aaagtacagc ctggtatggc ataacagccc gaggaagcaa ggtgatgcca ggtgattatc
    38161 agtatgttgt gacctatcgt gacgaacatg gtaaagaaca tcaaaagcag tacaccatat
    38221 ctgtgaatga caaaaaacca atgatcactc agggacgttt tgataccatt aatggcgttg
    38281 accactttac tcctgacaag acaaaagccc ttggctcatc aggcattgtc cgcgaagaag
    38341 tcttttactt ggccaagaaa aatggccgta aatttgatgt gacagaaggt aaagatggta
    38401 tcacagttag tgacaataag gtgtatatcc ctaaaaatcc agatggttct tacaccattt
    38461 caaaaagaga tggtgtcaca ctgtcagatt attactacct tgtcgaagat agagctggta
    38521 atgtgtcttt tgctaccttg cgtgacctaa aagcggtcgg aaaagacaaa gcagtagtca
    38581 attttggatt agacttaccg gtccctgaag acaaacaaat agtgaacttt acctaccttg
    38641 tgcgggatgc agatggtaaa ccgattgaaa acctagagta ttataataac tcaggtaaca
    38701 gtcttatctt gccatacggc aaatacacgg tcgaattgtt gacctatgac accaatgcag
    38761 ccaaactaga gtcagataaa atcgtttcct ttaccttgtc agctgataac aacttccaac
    38821 aagttacctt taagataacg atgttagcaa cttctcaaat aactgcccac tttgatcatc
    38881 ttttgccaga aggcagtcgc gttagcctta aaacagctca agatcagcta atcccgcttg
    38941 aacagtcctt gtatgtgcct aaagcttatg gcaaaaccgt tcaagaaggc acttacgaag
    39001 ttgttgtcag cctgcctaaa ggctaccgta tcgaaggcaa cacaaaggtg aataccctac
    39061 caaatgaagt gcacgaacta tcattacgcc ttgtcaaagt aggagatgcc tcagattcaa
    39121 ctggtgatca taaggttatg tcaaaaaata attcacaggc tttgacagcc tctgccacac
    39181 caaccaagtc aacgacctca gcaacagcaa aagccctacc atcaacgggt gaaaaaatgg
    39241 gtctcaagtt gcgcatagta ggtcttgtgt tactcggact tacttgcgtc tttagccgaa
    39301 aaaaatcaac caaagattga taacaaagcg caaagagaca acaaatcatc ccttttaaca
    39361 gtctgacctt tctttgagat tgagctggca gatgatcatt tcttaacaaa agttaaagag
    39421 ttgagcaaag taaggaacag acataaagac cataaaagca agccagtttg gcttgctttt
    39481 atggtgttat tgtcgtgtgc tacgatgact aaaaaagaac aacgaacaat gacctcgaat
    39541 caccaaaagg acttgatttg atagcttccc tatgctatac taagcctatt ctttcaagaa
    39601 ggactccttt tgtggaaaag actgaaaaaa aggacactaa tctatccaaa aactccagat
    39661 gagtagctgc aaacgatgcc tttgctgtga tcaaggtggt aaagcagact gacttgtcac
    39721 tatctgagca ggtgtcaaaa aaagaagctt tggctttgtc attcaacata ctgagcttca
    39781 gtctttcaga acggagacac ccccagtttt gtactcaaaa agcataaaca gctctgctga
    39841 tgtgacatct gatttctacg tgcgcagtga gccaaaacag ctgctgtttg ctatggaaaa
    39901 agtggcgcca cgcggaattt ttgagaccct aggctcaaaa ataaagcata gaattccaaa
    39961 ggaagtcgct gccgtaagca ccacctaagg aaagttatcc caaaagactt tttctgcaat
    40021 ttgaaatact gaatttcagt attttttctt ttgtaactaa gagcaatgaa tgaggtaaaa
    40081 aaaggcaaca atgtcaaaaa agaaacgaaa acactatcag gtttatgagg gattacgctg
    40141 tgctatgacg ctttgtttta tcagtggcta tgtcaatgcc tttacctata tgacccaagg
    40201 aaaacgtttt gcaggtgttc agacaggcaa ccttttgtca tttgccattc gcttgtcaga
    40261 acagcaactg aaggaggcac tgcaattttt acttcccatg atcgttttta tgcttgggca
    40321 gtcctttact tatttcatgc accgctgggc cactaaaaaa gggctccact ggtacttatt
    40381 atcaagtgtt atcttaacag ggattgcttt tggaacggcc ctattcacgc cttttcttcc
    40441 ttccaatgtt acggtagcag ctttagcctt ttttgcctct attcaggttg atacgtttaa
    40501 aaccttacga ggagctagct atgctaatgt gatgatgaca ggaaatatca aaaatgccgc
    40561 ctatttacta accaaaggct tgtacgaaaa aaatcacgaa ctaacacata tcggacgcaa
    40621 caccttaata gtcatcttgg cttttgctgt tggggtggtt tgttcgacgt tgctttgcat
    40681 agcttatggc gagtatgctt tgatgcctat tttaatgcct ttactctatg tcaattacct
    40741 gttggctcag gaattttatc acattcaaac caaaatcaaa ccgattaaaa gaacttaatg
    40801 gacttgtgga gaaagaaagt tcatttaggg taagttttta ctcaccctcc tttgattgac
    40861 tggttttgct gccccttaaa atatgatata atagaccatt gatagctgtt acttaacaga
    40921 aggctgataa aaaaggttcc attagccttg agggttgaat ttaaacagga aaaaacgaaa
    40981 tcctacacta tgcatctcaa tcgtactttc atttgagtat ggttctgaaa ggaaactatg
    41041 atgaaaaaac cattttacat tacgacaccc atttattacc catctgggaa attacacatt
    41101 ggttctgcct atacaactat tgcttgtgat gtcttggcac gctacaagcg cctgatgggc
    41161 catgaggtct tttacctcac aggactagat gaacacggac aaaagattca aaccaaggca
    41221 aaagaagctg gtattacacc tcaaacctac gttgataaca tggcaaagga tgtcaaagcc
    41281 ctttggcaat tgctagatat ttcctatgac acatttatcc ggacaacgga cgattaccac
    41341 gaagaggtcg ttgctgctgt ctttgaaaaa ttgctggcgc aagatgacat ttaccttggc
    41401 gaatactctg gttggtattc tgtctctgat gaggaatttt tcactgaaag ccaattaaaa
    41461 gaagtcttta gagatgaaga cggtcaggtt attggaggga ttgccccatc aggtcatgag
    41521 gtcgagtggg tctctgaaga atcctacttc ctacgcctta gcaaatacga tgatcgtctt
    41581 gtggccttct ttaaagagcg acctgacttt atccaaccag atggtcgtat gaatgaaatg
    41641 gtgaaaaact ttatcgagcc aggcctagaa gatttggctg ttagccgtac tacctttaca
    41701 tggggtgtac ctgttccatc agacccaaaa cacgtggttt atgtctggat cgatgccctc
    41761 ttaaactacg cgacagctct tggttatcgt caagctaatc acgctaactt tgataagttt
    41821 tggaatggaa cggttttcca catggttgga aaagatatct tgcgttttca tagcatctac
    41881 tggccaattt tactcatgat gcttgattta ccaatgccag accgcttaat tgcccatggt
    41941 tggtttgtca tgaaagacgg taaaatgtct aaatcaaaag gcaatgtggt ctatccagaa
    42001 atgttagttg agcgctttgg acttgatcct cttcgttatt acctcatgcg ttctttgcca
    42061 gttggttcag acgggacctt tacccctgaa gattatgtgg gtcgtatcaa ctatgagtta
    42121 gccaacgacc ttggcaattt attgaaccgt accgttgcca tgatcaacaa gtattttgac
    42181 ggcacggtgc cagcttatgt tgacaatgga actgcctttg atgctgactt aagtcaactg
    42241 attgatgctc agctagcaga ctaccataag cacatggaag cggtggacta cccacgcgcc
    42301 ttggaggctg tttggaccat catcgctcgt accaacaaat acatcgatga aaccgctcct
    42361 tgggtgctcg ctaaagaaga tggtgacaaa gctcaactag ccagtgtcat ggcacactta
    42421 gcagccagct tgcgtctggt cgctcatgtg atccaaccct ttatgatgga aacatcagct
    42481 gctatcatgg cccagcttgg tttggaacct gtttctgatt tgtcaacact tgctctagca
    42541 gattttcctg ccaatacaaa ggttgttgcc aaagggacac cgattttccc acgtcttgac
    42601 atggaagctg agattgacta catcaaagcc caaatgggtg acagctcagc tatttcccaa
    42661 gaaaaagaat gggtccctga agaggttgcg ctcaaatctg aaaaagacgt catcacattt
    42721 gaaacctttg atgctgtgga aatccgtgtg gcagaagtca aggaagtgtc aaaagtggaa
    42781 ggttcagaaa aattgctgcg ctttagagta gatgcaggag acgggcaaga ccgtcaaatc
    42841 ttgtcaggta ttgccaaatt ttaccccaac gaacaagaat tagtgggtaa aaaactacaa
    42901 attgttgcta acctgaaacc gcgtaaaatg atgaaaaaat acatcagtca agggatgatt
    42961 ctatcagcag agcatggaga tcagctaacg gtcttaaccg ttgattcctc tgttccaaat
    43021 ggaagtatca ttggctgatg ctgacctatt attcaaaaaa cctaagcaag aacatgtgct
    43081 taggtttttt tgattgcctt caagaaagac gaataacgag atgggtgata tgacatcaaa
    43141 agcataacta tcctaggacc tgtcctagta agattgctgt gcttttgaca agtgaacaga
    43201 gaggtcttaa aactatatct tgtcataaaa aagattgcaa aacactatat cttgtgttat
    43261 tatggttttt ataaaagtta agcatactta actttagcca aacaaaagag gacactagag
    43321 cgtgtcccac aagacagttg agcccttcgc ttttttagta gagtaggagt gctcaagtca
    43381 aaaagagaca tcccatcaaa ccattgctgt ttagcaggtc tttttgtgtt gctcaaaaaa
    43441 acaattcctg tctgtttggc gtctagtgat gacttaggct tagcgctact agacttttgt
    43501 ttgaaaaata cctcaagcct tgaaggagac aaatgaccca acattattat gaacgttcgc
    43561 aatccccaat tgaatacgcc ctatctgaaa cacaaaaaca gttgcgctcc atcaattgga
    43621 attaccttaa tgatgacaaa gatttagaag tgtggaaccg tgtcactcaa aatttttggt
    43681 tgcctgaaaa ggttcctgtt tctaatgatt taaattcttg gcgctccttg ggtgaggatt
    43741 ggcaacagct catcacaagg acctacactg ggttgacctt actagataca gtacaagcaa
    43801 cggttggtga tgtggcacaa attcagcatt cacaaaccga ccacgaacaa gtcatctata
    43861 cgaactttgc ctttatggtg gggattcatg cccgctctta cgggacgatt ttttcgaccc
    43921 tttgcagcag cgagcaaatc gaagaagcac atgaatgggt ggtctcaaca cagagcttgc
    43981 aagaccgtgc ccgtgtcttg attccttact atacgggtga tgaccctttg aaatcaaaag
    44041 tagcagcagc tatgatgcca ggatttttgc tgtatggcgg cttttacctt cctttttacc
    44101 tatcagcacg tggtaaaatg ccaaacacat ctgatatcat ccgcctgatt ttacgcgaca
    44161 aagttattca caactattac agcggttaca aataccaaca aaaagtagca cgtcttagcc
    44221 cagaaaaaca ggctgaaatg aaggcctttg tctttgactt gctctatgaa ctcatcgacc
    44281 ttgaaaaagc ttacttgaga gaactgtatg caggctttga tttggcagaa gatgctatcc
    44341 gttttagcct ttataacgct ggaaaattcc ttcaaaattt aggctatgag tcgcccttca
    44401 cagatgaaga aacgcgcgtt tcaccagaag tatttgctca gctatcagcg cgtgcagacg
    44461 aaaaccatga ctttttctca ggcaatggct catcttatgt gatggggatc accgaagaaa
    44521 ccacagatga cgattgggag ttttaagaaa tggcagagct gattattgtg tatttttcat
    44581 ctaagtccaa taacacccat cgcttcgtcc aaaaattagg cctgcctgct cagcgcatac
    44641 cagtagataa taggccactt gaagtttcca cccattacct tttgattgtg cctacctatg
    44701 cagctggtgg ctcagatgcc aaaggagccg tttctaaaca agtgattcgt tttttgaata
    44761 atcctaacaa tcgtaaacat tgtaagggag tgatttcttc tggaaatacc aattttggcg
    44821 atacctttgc cttagcaggc cctatcattt ctcaaaaatt gcaggtcccc ttattacacc
    44881 aatttgaact attaggcact gctacagatg tcaaaaaggt acaggcgatt tttgcccgct
    44941 taaagcatca cacacacgac aagcaaaaac aaaccaacaa cctgattaca gaaaggacac
    45001 acccatgtca caaaccaatg cgtcatacct ctcattaaat gcactgaccc gttttaaaaa
    45061 accagacggc agctaccatt ttgatagcga taaagaagct gttaggcgct acctggaaga
    45121 acatgtctct cccaatcaaa tggcttttaa ctcactagaa gataagctag cttacctcat
    45181 caatgaaggg tattatgagc aggccatttt tgatgcctat cctaatgacc ttatcaagga
    45241 ggcctttcat tatgcctatc aacaaggcta tcgcttttta aatctcatgg gcgctatgaa
    45301 attttaccag tcctatgcct taaaaacctt ggacggtaag cagtatctag aaacatttga
    45361 agaccgtgct gtgatgaatg cactcttttt agctgatgga gatcaaacct ttgtctttga
    45421 cgtgattgat gccatcttgc accgacgctt tcaaccagct accccaacgt ttttaaatgc
    45481 cggaaaaaaa cgccgtggcg aatacatttc gtgctatctt cttcgtgtcg aagacaatat
    45541 ggaatctatc tctcgcgcca ttagcaccag cttgcaactc tcaaaaagag gaggcggagt
    45601 cgccttgtgc ctcaccaacc tcagagaaat cggagcaccg ataaaaggga ttgaaaatca
    45661 ggcaacaggc atcgttcctg tcatgaaact actagaggac tctttttcct atgccaatca
    45721 actaggtcag cgccaaggag caggtgcggt ttaccttcat gcccatcacc ctgaggtttt
    45781 gaccttttta gacaccaaac gtgaaaatgc cgatgagaaa attcgcatta agtccctagc
    45841 attgggactt gtgatcccag atattacctt ccagttagcc aaagaaaaca aagacatggc
    45901 actcttttca ccctatgata tcaagcgtgc ctatggcaag gacatgtcag acatctccat
    45961 cacagaggaa tacgataaac tgctagccaa tccagccatc aaaaagacct acatcagtgc
    46021 tagaaagttc tttcagttaa ttgcagagct acattttgag tcaggctacc cttacctctt
    46081 gtttgatgac acggtcaata agcgcaaccc tcatgccaaa aaaggcagaa tcgtcatgtc
    46141 taacctgtgt tctgaaatcg ctcaagttag caccccaagc actttcaaag aagatttgag
    46201 ttttgaaacc attggagagg atatctgctg taatttaggg tctatcaata ttgcccaggc
    46261 tatggcagat gcgccacact ttgaacagct catcacaacg agcatcagag cacttgatcg
    46321 tgtctctaga gtgtctgacc tgaattgtgc gccttctgtt gagacaggca atgcagccaa
    46381 ccatgcagta ggattaggcg ccatgaacct acatggtttt ttggcaacca accacatcta
    46441 ctatgataca aaagaagccg ttgactttac agatctgttt ttccatgcca tggcctatta
    46501 tgcttttaaa gcctcttgcc aattagccaa agaaaaaggc gcttttgcag gattttcgtt
    46561 atcaacctat tcagatggca cttattttgc caagtatttg caagaagatg ccaagccaca
    46621 aacagccaag gttgccacat tgttgcaaga ttacggcttc acgttgccaa cggtggcgga
    46681 ttggcaagcc ctagtagcag atatcaagca gtttggccta gccaatgcgc acttgctagc
    46741 ggttgctccg acaggctcca tcagctactt atcatcctgc actccgagtc tacaaccagt
    46801 cgttgctcct gttgaggtcc gaaaagaagg tagcctgggc aggatttacg tccctgccta
    46861 tcagattgac caggctaact atgcttacta tgaacgtggt gcctatgagg tcggcccaaa
    46921 agccatcatc gacgttgtag ctgctgccca aaaacacgtt gaccaagcca tctcattgac
    46981 cctgtttatg accgatcaag cgaccacacg tgacctgaac cgctcctata tccaagcttt
    47041 taagcaaaat tgtgcatcga tctattatgt gcgagtgagg caagacgtcc tagcaggcag
    47101 cgaacagtat gacgaggata gtcttgtgac agctcctgga gctagtgatg agaccaccac
    47161 agagtgtcaa agctgtatga tctaacagct actagaaagc tctcgtgtcc tatcacatgc
    47221 ataggatcag tgcactcgac ctttcaagac aagcaagcat cagctcttgc ttgtcttttt
    47281 ttggcctcaa agcccgttag tctgctgcta tgcgaggctt ttttgagcat cagaacgtca
    47341 aaaaaaagga catggagtcc tttttttggc gatcggtgtt gaggccgtca caaactgccc
    47401 ttgaaatacg cttctatgtg gagctttttt tggtcctgtg acacgtaagc tccaacgacc
    47461 tccagctgac agcctcttgg aaacaagagc tcaacctctg aaggcacagc cgaataaggc
    47521 tcgacaaagg ctgccttggc cccttttttg acacagatgc gcacctccac cggtcgatgg
    47581 gtcatggcgc cgtttttcaa ggctgtcgtg ctcataaaac tgtgcttggt gtagcgtgta
    47641 ccaagcttga tcttacaaag gatatgaggg tcaaactgat ggttacggta gtaagacgtg
    47701 agatcagcat gtgaaacacc gatatcacgc aaaaacgtct cgtatacata gcggtatact
    47761 acaatgttcc acgggatgac tagccggtga gtcgcagcat cgagctgggc cacctgatcc
    47821 cttagctcag gcgtgagttg gctgagctca cctttggctt tgtctaggct ggtgttgata
    47881 gggcctgcct gctgacaggt ataagcagcc atcgcctctt gctcagccct tgggtagatg
    47941 agacccttgc cccatcgctt tttaatcagt gtctcagcgc ccttttgatc cgaaaaggta
    48001 aaataagcag gcgtcccctc acaaaccgcc cgctcatagt gctcacagac aaaaagctcg
    48061 accaagccct cagtttgcca gatcagacta aaacagctaa gaagaaggac aatagccagt
    48121 tgatagcgct tttttagcat agttccccct gacaccagcc caaaaggctg acccttcccc
    48181 tagacctttt ggctagctct tgacaccacc agtttctcag acaggctaat ctttttgtca
    48241 tctctttctc cctaaacaga ctctcttatc atgatattat gctattatga gcgatttgtc
    48301 aatccatagc aaccgttttt tagttagata agtaagcttt tgacctagag gcagacaaga
    48361 gtggcagtac ctcccgtttg ctgtggtggc taataaggac taacacaaaa tctcaaacgc
    48421 tttcaaaaac agctcctaag cttaaaaaag ctgatgaaca gatttgttga acttaagaga
    48481 gattgccatg attttctaat catttgatgt ttaaaatgat aaaaacatga acttgaaatg
    48541 aaataatgag aggagtaaac tagaaccata tcaaaaaata atgaaatgga gtggttttat
    48601 gaaaacaaaa tcaaaacgct ttttaaacct agcaaccctt tgcttggccc tactaggaac
    48661 aactttgcta atggcacatc ccgtacaggc ggaggtgata tcaaaaagag actatatgac
    48721 tcgcttcggg ttaggcgatt tagaagatga ttcagctaac tatccttcaa atttagaagc
    48781 tagatataaa ggatatttag agggatatga aaaaggctta aaaggagatg atatacccga
    48841 acggcccaag attcaggttc ctgaggatgt tcagccatct gaccatggcg actatagaga
    48901 tggttatgag gaaggatttg gagaaggaca acataaacgt gatccattag aaacagaagc
    48961 agaagatgat tctcaaggag gacgtcaaga aggacgtcaa ggacatcaag aaggagcaga
    49021 ttctagtgat ttgaacgttg aagaaagcga cggtttgtct gttattgatg aagtagttgg
    49081 agtaatttat caagcattta gtactatttg gacatactta agcggtttgt tctaaaaaat
    49141 attttttata gtacaatttt ttagcaaatt gttttgaaaa agaacacttt ataaatagtt
    49201 catatttatg gtataatacc taattcaagt atttttaaat ggttggtgaa ttcttggctg
    49261 ataaaattct ttattagtgt tgagcagtcc cactgtttta aaacaatttt ctgctataat
    49321 tgggataatc cctttagtag tcaggccacc tctgataggg gtggcttttt tgggttttag
    49381 tgacccgtgt gttgagaaag gagcatttat gccagaaaag aaagtgttgc ccgtcctaag
    49441 catagtggtg agttttgtct gcctgcttga tcatatcttc ttttttgcta cttatagtta
    49501 tctctactta ccgcttgtag caagtagtct agctcttatc ggtctggtgc aaaacaaagg
    49561 caggcaaaag gtgtggtcgc tagctggact cgtcctagct gctctagccg tggcagtagt
    49621 ggcctatgcc tactatgacc caaccgtcat cccctactaa ccttaaccac tccttcgcac
    49681 gcaatcaaca gcacaaaaag cacccctgac aggtgctttt tgtgctttag ccaacctata
    49741 gcctcgtaat ccctcactga aacagtccct taaagttcca ccactgatct tggatgctct
    49801 cacttaaccg ctgatgccaa ggtttatggt cttcttctct tatctgcttt agcatgcttt
    49861 cttctagctg tttttgttgc ttttcctctt cctttttttg aagtgcttct tcagcttctt
    49921 tttctttttt tatagtatca gagtctctat agatatagct accgacataa aatccgtctt
    49981 ctcctttaaa tgagaaagtt aatttttctc ctttcttcaa aggatcttct cctaagttaa
    50041 gaatataagg tactcctgta taatattgac gataaggatc tgtttttgtt atattttttt
    50101 cgagaatagg actagctaaa attcctcgtt ctttactgtt atgatcacat tgtctgtaaa
    50161 gatataccca ccatccttga gggatatata tttttactac tttactacca acatataagt
    50221 cttctagagt gattgtgttg ccactataat ctttaatttt ttgttgaggt ccatccaaat
    50281 caaattttac tggcttttgt gtactacttt gttcatcctc cgctctgacg ctactagtta
    50341 ccccgatggc aaagagtgcc agtagcaaag ttaatgtctt tttcatttcg ttctctcctt
    50401 ttgctcattt tttaagacaa ggcttattat accaattctt gcgctaaaaa gatagggatt
    50461 gattggctga ggcgatcgga gaagtcctag atttctctga gtggctgcca ctcacttaat
    50521 gtttcactat tttattggga tatgctaata cttcaagata tctttatatc gtctatagtc
    50581 aagagcctat ttgacacttt atctctatgt aaataatttg cacactctgt taagtgctct
    50641 ttttggtcgc ttatcctctt ttaaagttgt tttatgtcag ttgaaaacga tgtttcttga
    50701 gtaacctaac ttacctgata tctcgtatcg tgatacgtta tttatcaata ttatgaaata
    50761 gaacaacgat attaggcatt cttttcgatt aacttcgaat agcttaataa atgtttatca
    50821 tctgcaattt ttagcataat ttaaagaaat acgactgtat cttgataggt actgtttttt
    50881 gatgtcttta acaatatatt ctcaaatttt caagaataac taccttgcta aagatatatt
    50941 ttatattttt aattttctca
//
LOCUS       DAXWIQ010000009        49634 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000009 DAXWIQ010000000
VERSION     DAXWIQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49634
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.009"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            3..1313
                     /gene="rocA"
                     /locus_tag="ACGEUI_000499"
     CDS             3..1313
                     /gene="rocA"
                     /locus_tag="ACGEUI_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RocA"
                     /protein_id="HFF8165360.1"
                     /translation="MIEIMLTLKLFSFVSAIPLRLKNIFYLSLSMVLFQVVFWAFFPD
                     HFILDVVMLAQFLFFALIALYYGKSIKAKFLMFYAFFPLVSISLVKRFIVFFVMPLFG
                     MPYSVVKHNTLLIYSITCFSIFLIYRCIQVFHFDFSTWRQYFQSHRASKLLVFTNSSM
                     ALYYLCVQGIDVMSPSLSGLATTTARSIIVLFYFILFLTLLIHLERYVKQNSIEAIVQ
                     QKEYRELINYSQHLGLLYQDIQELRRLLTTVSSRLKIGIEQNDISIVRLTYEGILNAE
                     KNNAKDDRLDLTCLDKLQVEAIRHIVLAKLIEAKNKKLKVEVSIPNCIATFFLEVVDF
                     TKLLSFLLDNAIEMSLETKQPCLSIAFLDQNHKLVIVIQSSTKQGQDDSQSVFAIPAL
                     KKRDDWQFDLRNVTTILNRYDYLTISSQIHDGILTQLIEIAKPD"
     gene            complement(1414..4119)
                     /locus_tag="ACGEUI_000500"
     CDS             complement(1414..4119)
                     /locus_tag="ACGEUI_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFF8165361.1"
                     /translation="MATKKVHIISHSHWDREWYMAYEQHHMRLINLIDDLLEVFQTDP
                     DFHSFHLDGQTIILDDYLKVRPEREPEIRQAIASGKLRIGPFYILQDDFLTSSESNVR
                     NMLIGKEDCDRWGASVPLGYFPDTFGNMGQTPQLMLKAGLQAAAFGRGIRPTGFNNQV
                     DTSEKYSSQFSEISWQGPDNSRILGLLFANWYSNGNEIPTTEAEARLFWDKKLADAER
                     FASTKHLLMMNGCDHQPVQLDVTKAIALANQLYPDYEFVHSCFEDYLADLADDLPENL
                     STVQGEITSQETDGWYTLANTASARIYLKQANTRVSRQLENITEPLAAMAYEVTSTYP
                     HDQLRYAWKTLMQNHPHDSICGCSVDSVHREMMTRFEKAYEVGHYLAKEAAKQIADAI
                     DTRDFPMDSQPFVLFNTSGHSKTSVAELSLTWKKYHFGQRFPKEVYQEAQEYLARLSQ
                     SFQIIDTSGQVRPEAEILGTSIAFDYDLPKRSFREPYFAIKVRLRLPITLPAMSWKTL
                     ALKLGNETTPSETVSLYDDSNQCLENGFLKVMIQTDGRLTITDKQSGLIYQDLLRFED
                     CGDIGNEYISRQPNHDQPFYADQGTIKLNIISNTAQVAELEIQQTFAIPISADKLLQA
                     EMEAVIDITERQARRSQEKAELTLTTLIRMEKNNPRLQFTTRFDNQMTNHRLRVLFPT
                     HLKTDHHLADSIFETVKRPNHPDATFWKNPSNPQHQECFVSLFDGENGVTIGNYGLNE
                     YEILPDTNTIAITLLRSVGEMGDWGYFPTPEAQCLGKHSLSYSFESITKQTQFASYWR
                     AQEGQVPVITTQTNQHEGTLAAEYSYLTGTNDQVALTAFKRRLADNALITRSYNLSND
                     KTCDFSLSLPNYNAKVTNLLEKDSKQSTPSQLGKAEILTLAWKKQ"
     gene            complement(4134..5420)
                     /locus_tag="ACGEUI_000501"
     CDS             complement(4134..5420)
                     /locus_tag="ACGEUI_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFF8165362.1"
                     /translation="MTRPIPTSVSQFMAKVESLCGDQHPDWALNFKTSFTNTLETTLK
                     TYEDGTSFLLTGDIPAMWLRDSTAQMKPYLFLAKEDEEIRKIIAGLVKRQFRYICIDP
                     YANAFNEEANEKGHQTDHTQMNPWIWERKYEIDCLCYPIQLAYLLYRETGSTDQFNDD
                     FHRGVELILDLWTVEQDHAQSPYLFERDTWRKEDTLTHAGKGSPVAPTGMTWSGFRPS
                     DDACQYGYLIPSNMFAVVVLSYLEDLYNNLFHNEPVATRAKQLKEAIQSGIADHALVQ
                     NSKGETIYAYEVDGLGQFSIMDDANIPSLLAAPYLGFCTKDDPIYLATRRTILSQENP
                     YYYQGNAAAGIGSSHTPENYIWHIALALQGLTALDQDSKKEMLDLLVATDAGTHLMHE
                     GFDVNDPYQYTREWFSWANMMFCELLLDYLGFSIAP"
     gene            5553..6593
                     /locus_tag="ACGEUI_000502"
     CDS             5553..6593
                     /locus_tag="ACGEUI_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8165363.1"
                     /translation="MTKLLYKKIYHFIKEQIASGRLQIGDRLPTEKELSEQFSVSRIT
                     SKRALVELEQEGLITRSRGKGSFVAKKQVTSPEANKDLLLILPFASDYELGDYAKGIM
                     TSIAETGYRLMVQLASTVRLDTLSDYAGIIYYPEDVNHSIDFLFYCDHHHIPLVLLDK
                     SLDLFQFPSVVADNKGGACQLTQHLIEQGCDQISFVATGSFGEVSSVRDRYLGYLAAM
                     AESSLKSHYFPKTETETTDAYLDRLVKTLSETTATKRGLVVENDWLAIQLIQKITQVG
                     LSIPDQVAIVGFDNSQASRLLSPKLSTAAQDFYQMGQEAAKVLLQIIHEPDQAVVSCQ
                     LPVQVFIRESSH"
     gene            6644..8338
                     /locus_tag="ACGEUI_000503"
     CDS             6644..8338
                     /locus_tag="ACGEUI_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase"
                     /protein_id="HFF8165364.1"
                     /translation="MTIYHALKDYQEVMTRGDYLVFDTPLTCRFIGRFFRFENQKALL
                     AELATSKYFQWIEEGQAEVTMKHFFNRQLAKDAFTLKISEDKEIIIESQNLRGFRYAQ
                     EALLKVMTFKGDKLYLPIVSVKHSPSFAMRGIIEGFYGTPWTREERLDCLRFIGNKRM
                     NTYMYAPKDDDYQRKLWRDLYPEDWVTYFKELLAVAKEEGLDFWYMISPGLDFDYTKE
                     ADYQLLYQKLQQLLALGVCHFGLLLDDIDYQIVDAVERRFKKTAYAQAHLATEVHHFL
                     NQQHAAPELVICPTEYDNHHDSIYLQELSERIPKEVAFFWTGPSTLASQISQADIETM
                     AAVYQRPIIIWDNIPVNDYQKDPERLFLTPFANRSPFLCQPGYQVKGIVSNPMISWEL
                     SKLTLTDMSHYLWDANRYQPSHSWLETLTDYTEDTELALALQAFAWHNGNRHLHRDLP
                     FEVEEALLAKDVSTLSAWVAELVERVNTLRKLDKPAFQQAIAPWFERVAKDQDFWQAM
                     LNQEPQLETLYADLQEDKHRIGSDIPSRYYRIYYQQQDKLTANQGQVTQARPEDYA"
     gene            8362..9804
                     /locus_tag="ACGEUI_000504"
     CDS             8362..9804
                     /locus_tag="ACGEUI_000504"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8165365.1"
                     /translation="MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYD
                     YVGPDTASDAYHQIESDLTLLASLGHNSYRTSIQWTRLIDDFEQATINPDGLAYYNRV
                     IDACLANGIRPVINLHHFDLPIALYQAYGGWESKHVVDLFVAFSKVCFEQFGDRVKDW
                     FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRRGPAELSDGR
                     IGTILNLTPAYPASQSEADMAAAHFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLW
                     QSTPEELALIAENRVDYLGLNFYHPKRVKAPDAIPVISPSWSPEWYYDPYLMPGRRMN
                     VDKGWEIYPEAVYDIAIKMRDHYDNIPWFLSENGVGISGEDRYRDETGQIQDDYRIQF
                     LKEHLTYLHKGIEAGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASA
                     YWFKKVATHNRLISLEVMEEFGGSASHLSD"
     gene            complement(10150..10272)
                     /locus_tag="ACGEUI_000505"
     CDS             complement(10150..10272)
                     /locus_tag="ACGEUI_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165366.1"
                     /translation="MPGPFVDVIVADASVKGIGHQLMLELLEAMLVIANKQSLT"
     gene            complement(10415..11293)
                     /locus_tag="ACGEUI_000506"
     CDS             complement(10415..11293)
                     /locus_tag="ACGEUI_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8165367.1"
                     /translation="MSLLCIDIGGTSLKFALCHNGQLSQQSSFPTPSSLEKFYQLLDQ
                     EVARYSAYHFSGIAISSPGAVNKEKGVIEGASAIPYIHHFKIQEALEERLHYPISIEN
                     DANCAALAEATLGAGKGASSLAMLVLGTGVGGSLVIDGKIYHGAHLFGGEFGFMIMND
                     RYQTFSQLGTVVNMAKRYSAIVNNGKDYTGKAVLALAEQGDPLALKERQVFLQSLAIG
                     IFNIQHAFDPQLILIGGGVSQADFLLSAIEAELDKLYQAVEISDLRPQLAICHFKNEA
                     NLLGAAIDFYQEHKEA"
     gene            11489..12439
                     /locus_tag="ACGEUI_000507"
     CDS             11489..12439
                     /locus_tag="ACGEUI_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004219264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8165368.1"
                     /translation="MIRQTNKKTSFWKNVIKYRALLLMVLPGFIWFIFFFYIPVLANV
                     VAFKDFHYSAGGFMESLKESPWVGLANFKYLFASKDAWLITRNTIAYNVIFLLFNVFF
                     AIAFAIIMSELRNKRTVKVYHTMSLLPYFLSWVVIEYFVSAFLNTDKGFINQLLTGSG
                     ADPIKWYSNPTWWPLILLFMSVWKGLGYNSIIYYASVKGISDTYYEAAMVDGASKWQQ
                     IRNITIPQLLPMMSILLIINIGNIFKSDFGLFYVIPKNSGPLYDVTSVLDTYVYNALT
                     ATGDIGMASAASLYQSVVGTSILLVTNAIVRRMDPDAALF"
     gene            12449..13375
                     /locus_tag="ACGEUI_000508"
     CDS             12449..13375
                     /locus_tag="ACGEUI_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8165369.1"
                     /translation="MEKKKKVEKVNVRTFDRKTNAIFNILIGLFAISCIIPFIFVIII
                     SFTDESYLINHGYSFFPDVWSTKAYQYIFQGAMSHRIMRSFGISVFITVVGTFINTTM
                     TSTYAYAISRPYFPYRRFFTVYALITMLFAPGMVANYLVVSNLLHLKDTVWALILPMA
                     LGPFGILVMRTFFKKTVPDSIIESARMDGASEWMIFMKIVLPLAVPGIATISLFSALT
                     YWNDWFNALLYVQSENLYPMQYLLMKIQSNLQALAQNAGMSAQMADSLASLPKESVRM
                     AIVVIATLPIALTYPFFQKYFVGGLTIGGVKE"
     gene            13456..14901
                     /locus_tag="ACGEUI_000509"
     CDS             13456..14901
                     /locus_tag="ACGEUI_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8165370.1"
                     /translation="MKKWQKIVCVTGTVLAASSLAACESKSASKDSDVKLLMYQVGDK
                     PDNFDELMTIANKRIKEKTGATVDLQYIGWGDWDDKMSTIIASGENYDIAFANNYVVN
                     AQKGAFADLTTLMPKYAKKTYKNLDPAYIKGNTIDGKLYAFPVDANVYAQQMLSFNKE
                     LVDKYGLDISNIKSYADAENVLKQFHEKEPNTAAFAIGQVFSMSGDYDYPLTKTQPFA
                     VKIDEGKPTIINQYEDESFKNNLRLMHKWYKEGLIPTDAATNTEGYPLEGNTWFMREE
                     TQGPMDYGDTILTNAAGKDIVSRPLTKPLKTTSQAQMANFVVSSVSKNKEKAVEVLSL
                     LNSDPELLNGLVYGVEGKAWEKIGDKKIKLLDGYQPKMHMGAWNTGNNKILYTQESVT
                     DDMIAKRDQSIKDAKESPILGFTVDTKVIKTELSNISNVMNRYKASINTGTVDPDEAL
                     PKLLADLKGAGWDKVQKEVQKQLDDFVAKDK"
     gene            15207..15812
                     /locus_tag="ACGEUI_000510"
     CDS             15207..15812
                     /locus_tag="ACGEUI_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFF8165371.1"
                     /translation="MNGIEKTCFLIWKMIQLTLIFHLLSLAGLIVFGVGPAWQTIVTL
                     FLKTPQEENHYSLKRAVQLWKSYFKEANLRFGLFALTFLFLTFNLHWAVQFPSLFWFT
                     VSFLIVIAMVWLTMTYLYMVFYAVSYEINLWNNMKLAFISVFLSFKSFLLMLSVLLGI
                     TLVTWQYKGLYLFLTFGALVFCLDFVTKANRRLVDGVIDEC"
     gene            15802..17526
                     /locus_tag="ACGEUI_000511"
     CDS             15802..17526
                     /locus_tag="ACGEUI_000511"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8165372.1"
                     /translation="MSVKTLGRYVTNHFKQGFLINRLMKLYSLLIVSFFTLAAIGLSS
                     YSISSTYKRVDAEAQMRLEETLGRLQSQNDITLRVLDQLVGRSEDYHNLYQYMTLTPN
                     QYFSNVFEDWEKGKDTVLFSEEVRRLFDLYPDVTSMTLLLEDSSAYLYADKVIKTGRL
                     LYSKPEKVTGNVLVRSIRNPESGDVTGRLYLTFDKPPTLVETQQDHYLATFAFDYYGR
                     KLFHQGGRRFPHLEAEVKKAIKADRAADLSNLSKAYRMQYNRSGDLLAYVAVRKSYLL
                     AEAVRTVFVYGLVSLLLAWLLLQLLFRVFRNYIQQVSEITDTVEMVAAGDLSLTIDNS
                     HMELELYHISEAINQMLASIKAYIDEVYVLEVEQRDAQMRALQSQINPHFLYNTLEYI
                     RMYALSCQQEELADVIYAFASLLRNNISQDKMTTLKEELAFCEKYIYLYQMRYPDSFA
                     YHVKIDESVADLAIPKFVIQPLVENYFVHGIDYSRHDNALSIKALDETDYLLIQVLDN
                     GRGISQERLADMEKRLQEHQTTGNSSIGLQNVYLRLFHHFRDRVSWSMAKEPNGGFII
                     QIRIRKDA"
     gene            17526..19010
                     /locus_tag="ACGEUI_000512"
     CDS             17526..19010
                     /locus_tag="ACGEUI_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8165373.1"
                     /translation="MYKVLLVDDEYMILQGLTMIIDWQALGFEVVQTARSGKEALAYL
                     TQYPVDVMISDVTMPGMTGLDLIEAAKTYHPQLQTLILSGYQEFSYVQKAMELETKGY
                     LLKPVDKAELQAKMKQFKDWLDAQQAESIRQEAYHDSLLTLWLTDELSEKEFQQLSQG
                     LPAAALTGFTVLYLDCQEWQAAIDAYFEREGQPFYLKKEEDKKVYLAVLLGKTDQAKA
                     FTYDLQKRFSRNMHQLILGETVDDWENVYESYNQVLKSLFYNCEVSPIRAKDKSKIEL
                     PESRLQFFAFNKALMIGDEPTILSKLEAIFDEMKTLNFSPEDVKHVSFLLFSDIYRQF
                     PILDKMTYLSMVKTIHDSQSIDCILRELKKVLDVTNQNNSPEKRYSDLVSETIDCIRK
                     EYHQELTLKAIADRLHVNGVYLGQCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSIN
                     EIAYETGYNTNHYFIKMFKKLNGLSPKEFRDRYKDNYQAIKGDQ"
     gene            19030..22446
                     /locus_tag="ACGEUI_000513"
     CDS             19030..22446
                     /locus_tag="ACGEUI_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFF8165374.1"
                     /translation="MTPDSKEQTVTGYQYHYIDQEGRKQPFNQGWRFLMADVACAQDP
                     SFDDSNWQVIHLPHDFSLTQPYTRNGEAESAYKLGGVGWYRHYLVLDEVLAGCHVAIT
                     FEGSYMETEIYVNGQFIGKHLNGYQEFTYDISDVVTFGAENLLAVRVENKVPSSRWYS
                     GSGLYREVSLSVLPQLHFVADQVAMTLADTAVQEKGQQKVDLRFALNQSIQTCHYQLS
                     LCLWEQSHCSKDKKLLYQETEVPLADLAFQRQYGLTLSLEELQLWSPDNPHLYDLELT
                     LYYQGQVIDCFCLETGFRQLTFMANQGLFVNGRAVKLKGVCLHHDQGGLGACAYEDAL
                     ARQLVLLKDMGANTIRSTHNPSSPKLRQLANRLGFFVIEEAFDTWTYAKNGNVNDFSN
                     YFHQTIGTENANYLQRVRSPETSWAQYSIEAMVWSAKNDPSVLMWSIGNELMEGFSAD
                     VSHYPELTRQMCQWITAIDTSRPITFGDNKLKEADFCWHEEVSQMATLLSQLDHPQGL
                     IGLNYADGKDYDRLHEEHSDWLLYGSETVSAITSRAYYKETKKVLDSGYHLTSYDHAK
                     VDWGAFASQAWYDTITRDFVAGECVWTGFDYLGEPTPWNKIDSGVVGLWPSPKNAYFG
                     ILDTAGFPKDSYYFYQSQWAQGQTTLHLLPVWQKDQLCFDEQGLVEVVVYSNAASVQL
                     MFEDEQGNLTDYGRKAFHTYSTPTGHTYQLYQGADAAKNPHENLYLTWRVPYQKGLLR
                     AVAYDISGKSIPKTSGRSQVRTYGSVAKLSWKAFEAPIDAPWELLYLDLSLLDSRGEL
                     VSHAQDWLQVQVEGPARLLALDNGNPTDHTPYQEPLRQAYGGKLLAILALTGEAGHIK
                     VTAKASQQLSSVFQTQVACRAPKEHLVTLPLEKYQFKKSRLTSQQEIGQSCLKTTQFL
                     SVLENKDTQQKAGKASTPHILITSYQHYLSLPLQVCSQAFDFKKGDDIFLPQWLQVKD
                     AQGSLVKKAFSMTWYLKEKIFAKPILVTGYVSCFGQSIPVEARLNLAQASKRRHQDLS
                     LVAKQSLAASQKRLTYCYQYDTVQAIGAISLQNHTGTSLSVSVAISYGSQSSIKQETL
                     MLADSHLSQLEEVIFFDDILTVLYLAVTLEVIEGPDLTEDSIQIGLWDLA"
     gene            22584..23462
                     /locus_tag="ACGEUI_000514"
     CDS             22584..23462
                     /locus_tag="ACGEUI_000514"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFF8165375.1"
                     /translation="MSERLSGHTLLVSLLATPIRHSLSPKMHNEAYAKLGLDYAYLAF
                     EVGTEQLADAVQGIRALGIRGSNVSMPNKEAILPLLDDLSPAAELVGAVNTVVNKDGK
                     GHLVGHITDGIGALRALADEGVSVKNKIITLAGVGGAGKAIAVQLAFDGAKEVRLFNR
                     QATRLSSVQKLVTKLNQLTRTKVTLQDLEDQTAFKEAIRESHLFIDATSVGMKPLENL
                     SLITDPELIRPDLVVFDIVYSPAETKLLAFARQHGAQKVINGLGMVLYQGAEAFKLIT
                     GQDMPVDAIKPLLGDE"
     gene            23697..24452
                     /locus_tag="ACGEUI_000515"
     CDS             23697..24452
                     /locus_tag="ACGEUI_000515"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8165376.1"
                     /translation="MVTKEVGHDFLARLGKKRLRPGGVEATNWLIEQGQFSQDCHVLE
                     VACNRCTTAIELAKTFGCQIDAVDLNPKVVKEAQERVAVEGLTRHITVSQASALSLPF
                     PDNHFDIVINEAMLTMLNAQAKEKALNEYWRVLKPGGRLLTHDVAYEDPKTRQVLDEL
                     RQTIHVNVAPLSLTGWQALYQEHGFSQVTYHHGKMSLMTPRGMIKDEGLMNTLNIMRR
                     GLKKENRQQFLKMRRFFTRTGKNLCYIAVKSVK"
     gene            24505..24843
                     /locus_tag="ACGEUI_000516"
     CDS             24505..24843
                     /locus_tag="ACGEUI_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFF8165377.1"
                     /translation="MSYINNIEHAKVLDLTQEVMIEQDQMLSRTLVQRQDLGITVFSL
                     DKGQEIGRHSSPGDAMVTILSGLAEITIDQETYRVAEGQTIVMPAGIPHALYAVEAFQ
                     MLLVVVKPEA"
     gene            24845..25150
                     /locus_tag="ACGEUI_000517"
     CDS             24845..25150
                     /locus_tag="ACGEUI_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFF8165378.1"
                     /translation="MIENLSQLVQYHDHQVASRSLSRLLGLSQSIVLYAMAQGESISQ
                     ETSPRDKVILVLEGQLIFDLEDQKQVLTQESLIAIPAQKVHHLEAKTDCKLLQVLLD"
     gene            complement(25585..26658)
                     /gene="aroB"
                     /locus_tag="ACGEUI_000518"
     CDS             complement(25585..26658)
                     /gene="aroB"
                     /locus_tag="ACGEUI_000518"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFF8165379.1"
                     /translation="MPQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQ
                     TVYHLYQTLFEEFAQQYNAFVHVCPPGGQSKSLERVSAIYDQLIAENFSKKDMIVTIG
                     GGVVGDLGGFVAATYYRGIPYIQIPTTLLSQVDSSIGGKVGVHFKGLTNMIGSIYPPE
                     AIIISTTFLETLPQREFSCGISEMLKIGFIHDRPLFQQLRDFQKETDKQGLERLIYQS
                     ISNKKRIVEQDEFENGLRMSLNFGHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAV
                     SKGLLPKEDLDSLLQVFERYQLPTTLERADVSATSLFDVFKTDKKNSEQHIIFILPTE
                     TGFTTLAINKDDHQFVEKLDSLL"
     gene            complement(26714..27466)
                     /locus_tag="ACGEUI_000519"
     CDS             complement(26714..27466)
                     /locus_tag="ACGEUI_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HFF8165380.1"
                     /translation="MIDIMSDFQNKTCSKNNFIVGPCSIESYDHIRLAASSAKKLGYN
                     YFRGGAYKPRTSAASFQGLGLQGIRYLHEVCQEFGLLSVSEIMSERQLEEAYDYLDVI
                     QVGARNMQNFEFLKTLSHIDKPILFKRGLMATIEEYLGALSYLQDTGKSNIILCERGV
                     RGYDVETRNMLDIMAVPIIQQKTDLPIIVDVSHSTGRRDLLLPAAKIAKAVGANGIMM
                     EVHPDPDHALSDAAQQIDYKQLEQLGQELWQD"
     gene            complement(27708..27778)
                     /locus_tag="ACGEUI_000520"
     tRNA            complement(27708..27778)
                     /locus_tag="ACGEUI_000520"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27744..27746),aa:Thr,seq:ggt)
     gene            27945..28199
                     /locus_tag="ACGEUI_000521"
     CDS             27945..28199
                     /locus_tag="ACGEUI_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HFF8165381.1"
                     /translation="MSYEKEFLKDFEDWVKTQIQVNQLAMATSQEVAQEDGDERAKDA
                     FIRYESKLDAYEFLLGKFDNYKNGKAFHDIPDELFGARHY"
     gene            28217..28612
                     /locus_tag="ACGEUI_000522"
     CDS             28217..28612
                     /locus_tag="ACGEUI_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFF8165382.1"
                     /translation="MAKQKNRKKALKLEMKRNGLLKKAGQVFDKAVESVETAVDKTIS
                     AGKNLVEKGSQTVENLTASKERLTLDEFAGQPVVAGIRSDLVETLYAEGIHSAQAFKE
                     WTEKDLLALKGIGPATVKKLVENGASFKK"
     gene            28974..29216
                     /locus_tag="ACGEUI_000523"
                     /pseudo
     CDS             28974..29216
                     /locus_tag="ACGEUI_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            29213..29776
                     /locus_tag="ACGEUI_000524"
     CDS             29213..29776
                     /locus_tag="ACGEUI_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8165383.1"
                     /translation="MNLIIIGAQASGKMTIGQEVARQTGMTLFHNHDSIDFVLRFMPW
                     SQESTALIERIRFAFFETFAKTGQDMIFTIVIDFNDPNDVAMLEKIQAVFQSYDQEVL
                     FVELKTDIEERLKRNRTENRLKHKPLKRNIEWLEQDIQSTMAYAVFNPEEPPKTLTHY
                     QKINNTQLTAAETAQLIIQKMTHIKEN"
     gene            29778..32426
                     /locus_tag="ACGEUI_000525"
     CDS             29778..32426
                     /locus_tag="ACGEUI_000525"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFF8165384.1"
                     /translation="MTELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIPP
                     PNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQGI
                     SRYDLGRDKFLDKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVFV
                     DLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVATT
                     RPETMFGDVAVAVNPEDPRYKDLIGKNVILPIVNKLIPIVGDEHADPEFGTGVVKITP
                     AHDPNDFEVGQRHNLPQVNVMNDDGTMNELAGDFAGMDRFEARQATVAKLEELGALVN
                     IEKRVHSVGHSERSGAVVEPRLSTQWFVKMDELAKQAMDNQETDDRVDFYPPRFNDTF
                     LQWMENVHDWVISRQLWWGHQIPAWYNAEGEIYVGEEAPEGDDWTQDEDVLDTWFSSA
                     LWPFSTMGWPDTDVEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVLI
                     HGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADSLRWFLSNGSAPGQDVRFSYEKMDAS
                     WNFINKIWNISRYILMNNEGLTLEDAESNVAKVAASEAGNVTDQWILHNLNETIAKVT
                     ENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVITRSVLLYTLDKIL
                     RLLHPIMPFVTEEIYAQYAQGSIVTVDYPVVRPAFENEAAHKGVESLKDLIRAVRNAR
                     AEVNVAPSKPITILVKTADSELEDFFNSNINYIKCFTNPEKLEISSAIAAPELAMTSI
                     ITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLGNERFVANAKPEVVQKE
                     KDKQADYQAKYDATQERIAEMKKIKS"
     gene            32580..34988
                     /locus_tag="ACGEUI_000526"
     CDS             32580..34988
                     /locus_tag="ACGEUI_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFF8165385.1"
                     /translation="MRMILAHYDCKKDKKQSLDEHLWHVACSSRQEASIIGQGDVLFL
                     IGLYHDLGKADRTFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKNRGVDKNERMTFN
                     EMVGYVISAHHGMYDLCYYFDDAEYYGFNKFKNRINRDLDGYHYHEDIKGYALKLEKK
                     LCDYGYKDLRELIDKAFDNYQQAMSSLNWQDKSEWDYYQSCMVRLYLSLLKNADILDT
                     VNAYGLKISPMDKTERSFLKHSYLAAIEQKYASFGQPNNQLNTIRTEIAERVKERGKR
                     DSKGIYRLDLPTGAGKTNLSMRYAFHQLVHHDKSRFFYITPFLSVLEQNASEIRKVTG
                     DLGVLEHHSNVVKQANEDDDDKDSLLSAYLSDSWDSQVVLTSMVQFFQTLFKTKSANL
                     RRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFLNKVMDTTIVLCTATQPAYDSSEID
                     HRICYGGNLGELAEIVELTIEEKQIFSRTELRKFDDSDQKVHLTDVINLILGEENSVL
                     AIFNTKKTVHNCYTMLKDMTDRPVYQLSTNMCAQHRLDLIAKIKTELQNNIPIICIST
                     QLIEAGVDVDFHRVIRSYSGIDSIVQAAGRCNREGKRDKGQVTLVNLTNEEENISRLT
                     EIKTKKEATESILHKIGSPIDISTLNRDFFEYYYANNQGLMDYPLEDNLSIYDYLSLN
                     IYQTANKKFKGKLKQAFKTAGAKMNLINNDMIGILVPYGEAEKKLAYLEELGVSHFLS
                     AKDYQTIKSLLKELQPFTVNVRENDPLFETTKSYLNGQILVLTSEYYDTERGVKYDSA
                     SFYF"
     gene            35121..35849
                     /gene="cas5c"
                     /locus_tag="ACGEUI_000527"
     CDS             35121..35849
                     /gene="cas5c"
                     /locus_tag="ACGEUI_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HFF8165386.1"
                     /translation="MYRSRDFYVRVSGQRALFTNPATKGGSERSSYSVPTRQALNGIV
                     DAIYYKPTFTNIVTEVKVINQIQTELQGVRALLHDYSADLSYVSYLSDVVYLIKFHFV
                     WNEDRKDLNSDRLPAKHEAIMERSIRKGGRRDVFLGTRECLGLVDDISQEEYETTVSY
                     YNGVNIDLGIMFHSFAYPKDKKTPLKSYFTKTVMKNGVITFKAQSECDIVNTLSSYAF
                     KAPEEIKSVNDECMEYDAMEKGEN"
     gene            35849..37744
                     /gene="cas8c"
                     /locus_tag="ACGEUI_000528"
     CDS             35849..37744
                     /gene="cas8c"
                     /locus_tag="ACGEUI_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HFF8165387.1"
                     /translation="MDFFTSLLKTYEKAELADLVDHQKRNNEPVLLPIYHTSLKSNGK
                     NIISVKLDKDGQFHKAEFMADKQMIIFPVTADSVARSGSHPAPHPLVDKFAYYSAEMG
                     QIQYDSFHKQLNNWIDYCEEGDVKKFLTFVQQFILKPEFLTLILDSLIGPDYQHNQLK
                     VTFCDATGKEKLIDLSACFLEFSIDQFQGFKNESVSTFKALHQSYISFVEANRENLGI
                     CNISGREEQLTDKHRGLMGNAKIISVSNKREAYKGRFREREDVFSVGYETSEKIHLML
                     KYLLENKNTSTWLGSSQYLINWFSDDLTNDSRLDIVSPIFDDGLEEEDDDDTPPVITL
                     ATEDNKRIGKSFIKGQKLFANDATYYVAILNKTSNGRIALKYFRQLQASQLLTNLNKW
                     QETYSWESRSKFGKSRLRTPTFHDILNVSYGVDRDRFLELDNDNFKSDQIQKLVASLI
                     DGKPMPQSIVKKLGNNVKERHRYRKHWYQVEQVCLAILHKQNGEEFSPMLDHTNQNRS
                     YLFGRLLAIFELIETLRYGLDGNNNDRITNAERYWTAYTGQPTKLMMLLENKIKPYEE
                     PLKLNRRGSWMKLEKEKEEILELLNPLLETETMEKPLDYRFIFGYYAEKNYYYTKQNT
                     EVTESEE"
     gene            37749..38597
                     /gene="cas7c"
                     /locus_tag="ACGEUI_000529"
     CDS             37749..38597
                     /gene="cas7c"
                     /locus_tag="ACGEUI_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HFF8165388.1"
                     /translation="MLEHKIDFMVTLEVKEANANGDPLNGNMPRTDAKGYGVMSDVSI
                     KRKIRNRLQDMGKSIFVQANERIEDDFRSLEKRFSQHFTAKTPDKEIEEKANALWFDV
                     RAFGQVFTYLKKSIGVRGPVSISMAKSLEPIVISSLQITRSTNGMEAKNNSGRSSDTM
                     GTKHFVDYGVYVLKGSINAYFAEKTGFSQEDAEAIKEVLVSLFENDASSARPEGSMRV
                     CEVFWFTHSSKLGNVSSARVFDLLEYHQSIEEKSTYDAYQIHLNQEKLAKYEAKGLTL
                     EILEGL"
     gene            38599..39273
                     /gene="cas4"
                     /locus_tag="ACGEUI_000530"
     CDS             38599..39273
                     /gene="cas4"
                     /locus_tag="ACGEUI_000530"
                     /EC_number="3.1.12.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HFF8165389.1"
                     /translation="MVYAEDDYLMLSGIQHFQFCKRQWALIHIEQQWLDNEATAHGQV
                     LHTKADNPYIKEKRKELLVSRAMPISSAELGLSGIMDVVEFYKDDQGVSLRGKRGKWL
                     PKVVEYKRGKPKKDTRDIVQLVAQTMCLEETLDCDINEGCLYYHSVNQRVIVPMTSAL
                     RQEVKELAAEMHEVYQSQMLPKAAYFKNCQLCSLVDICKPRLSKKTRSVSRYINEAMT
                     SEEMDL"
     gene            39270..40295
                     /gene="cas1c"
                     /locus_tag="ACGEUI_000531"
     CDS             39270..40295
                     /gene="cas1c"
                     /locus_tag="ACGEUI_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HFF8165390.1"
                     /translation="MKKLLNTLYLTQEDFYVTKEGDNIVIKQEGKVLKRFPFRIIDGI
                     VCFSYLGVSSALVKLCTENQINLSFHTPQGRFCGRYIGSTNGNVLLRREHYRLSDREE
                     SLEYAKRFILAKISNSRKYLLRFKRDHRQQIDTKLFEAVNDELIWALEMVQAADNKDS
                     LRGIEGQAANQYFRIFNDLVLTDKKTFYFQGRSKRPPLDCVNALLSFGYSLLTFECQS
                     ALEAVGLDSYVGFFHTDRPGRASLALDLVEEFRSYIVDRFVFSLINKGQLQKKHFEVK
                     ENGSILLTENGRAIFIDLWQKRKHTEVEHPFTKEKVKLMLLPYVQAQLLAKAIRGDLE
                     SYPPFMV"
     gene            40306..40599
                     /gene="cas2"
                     /locus_tag="ACGEUI_000532"
     CDS             40306..40599
                     /gene="cas2"
                     /locus_tag="ACGEUI_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HFF8165391.1"
                     /translation="MMVLVTYDVNTETPAGRKRLRHVAKLCVDYGQRVQNSVFECSVT
                     PAEFVDIKHRLTQIIDEKTDSIRFYLLGKNWQRRVETLGRSDSYDPDKGVLLL"
     repeat_region   40748..41042
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=40815..40845
                     /rpt_unit_seq="gtctcacccttcatgggtgagtggattgaaa"
     gene            41202..41912
                     /gene="ccdA2"
                     /locus_tag="ACGEUI_000533"
     CDS             41202..41912
                     /gene="ccdA2"
                     /locus_tag="ACGEUI_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HFF8165392.1"
                     /translation="MTLSLVLMVSVFGAGLLSFFSPCIFPVLPVYLGILLDADDSKTI
                     TIFGKKLYWYGIVKTLAFIFGLSTIFVILGYGAGFLGNILYAVWFRYLLGALVIILGI
                     HQMGLITIKSLQFQKSLTFHNNKNRNGLFNAFILGLTFSFGWTPCVGPVLSSVLALVA
                     SGGNGAWQGGVLMIIYTLGLGIPFLLISFASGIVLKQFNKLKPHILLLKKVGGVLIIV
                     MGILLMTGTLNNLAQLFG"
     gene            41925..42548
                     /locus_tag="ACGEUI_000534"
     CDS             41925..42548
                     /locus_tag="ACGEUI_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFF8165393.1"
                     /translation="MKKGLLVTTGLACLGLLTACSTQDNMAKKEITQDKMSMAAKKKD
                     KMSTSKDKSMMADKSSDKKMTNDGPMVPDFELKGIDGKTYRLSEFKGKKVYLKFWASW
                     CSICLSTLADTEDLAKMSDKDYVVLTVVSPGHQGEKSEADFKKWFQGTDYKDLPVLLD
                     PDGKLLEAYGVRSYPTEVFIGSDGVLAKKHIGYAKKSDIKKTLKGIH"
     gene            42591..43697
                     /gene="msrB"
                     /locus_tag="ACGEUI_000535"
     CDS             42591..43697
                     /gene="msrB"
                     /locus_tag="ACGEUI_000535"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFF8165394.1"
                     /translation="MEHKWKLWLFIVGVVLLIGGASVLIQKGQAKVFSQGKPQLPAKK
                     VKSTKVDPNAPVDQKVIYLAGGCFWGVEEYFSQVDGVLDAVSGYANGRGDTTNYQLIH
                     QTGHAETVEVAYDANRISLKELLLHFFRIIDPTSLNKQGNDRGSQYRTGIYYTDKADL
                     AIIDEVFKEKAKDYKKKIVVEKAPLKHFIKAEDYHQDYLKKNPNGYCHIDINQATYPV
                     IDESKYPKPSATEIKEKLSADEYRVTQKNETEKAFSNRYWDSFDAGIYVDVVTGEPLF
                     SSKDKFESGCGWPSFSRPISPDVVRYKEDKSFNMTRTEVRSRSGNSHLGHVFTDGPKD
                     QGGLRYCINSLSITFIPKSDMEAKGYGYLLSSVE"
     gene            43785..44525
                     /locus_tag="ACGEUI_000536"
     CDS             43785..44525
                     /locus_tag="ACGEUI_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8165395.1"
                     /translation="MYSLLIVEDEYLVRQGIRSLVDFSQFKIDRVNEAENGQLAWDLF
                     QKEPYDIVLTDINMPKLNGIQLAELIKQESPQTHLVFLTGYDDFNYALSALKLGADDY
                     LLKPFSKADVEDMLGKLRKKLELSKKTETIQELVEQPQKEVSAIAMAIHERLADSDLT
                     LKSLAQQLGFSPNYLSVLIKKELGMPFQDYLVQERLKKAKLLLLTSNLKIYEIAEQVG
                     FEDMNYFSQRFKQLVGVTPSQYKKGGQA"
     gene            44522..46255
                     /locus_tag="ACGEUI_000537"
     CDS             44522..46255
                     /locus_tag="ACGEUI_000537"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8165396.1"
                     /translation="MKRYPLLVQLISYVFVIVIALITTLGLLYYQTSSRNIRQLIERD
                     TRQSIRQSSQFIDAYIKPLKETTSVLAKNTEIQAFASQIHQENDKQVLQLMKMVLATN
                     SDLQAAVLVTKDGRTVSTNSQLTMKTSSDMMAEPWYKAAIDRQAMPILTPARQLSLSS
                     KKEWVVSVTQEVVDRAGHNLGVLRLDIAYPTIKASLDQLQLGRQGFAFIVNDKHEFVY
                     HPKKSVYSSSKEMAAMKPYLAIQNGYTKDKTSFVYQKLIPNSQWTLVGVASLDQLHRV
                     QRQIFWSFLGTGLLACLICGFATVLVLRRWIRPIQQLQQVILAIQKGDRQLRAQETGS
                     PELTDLAQQFNALLDQIDSLMVAVADKEKAIGQYRLQALASQINPHFLYNTLDTIIWM
                     AEFNDSKRVVEVTKSLAKYFRLALNQGNEYIRLADELDHVSQYLFIQKQRYGDKLSYE
                     VQGLDVYADFVIPKLILQPLVENAIYHGIKEVDRKGMIKVTVSDTAQHLMLTVWDNGK
                     GIEDSSLTNSQSLLARGGVGLKNVDQRLKLHYGEGYHMTIHSQSDQFTEIQLSLPKMH
                     ELMADDTQENE"
     gene            46328..47407
                     /locus_tag="ACGEUI_000538"
     CDS             46328..47407
                     /locus_tag="ACGEUI_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFF8165397.1"
                     /translation="MSLATDYFSRQTPIVEKLMAYGFEKRDNGYFYNERFMEGEFEAQ
                     LRIDEAGNIWDRVIDCDLEEDYLPLQQAAWQGTYTGQVRAAYLELLERLSVACFEATP
                     FQSMQANRLAKHITKEWSDPMDYPFEKHPDLATYRVGGKWYAMIFSLLADKLDQIPER
                     LVGQTCEVMTVKVNPKAFPQLLQQEGIYPAYHMSKKNWISIILDDKVTDDKLWTLVTQ
                     SRQLVNPNGLSNPNGPDYWVIPANLKYYDIDAEFAANDVILWTQKAGIAVGDYVLIYI
                     TAPVKSIRYVCQVLETDIPNEGYRKNPNIDKLMRLRKCQQYKDGLLSFDLMKKHGVAA
                     VRGPRRLSPQLIAFLKEKEYFKENN"
     gene            47421..48137
                     /locus_tag="ACGEUI_000539"
     CDS             47421..48137
                     /locus_tag="ACGEUI_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HFF8165398.1"
                     /translation="MVEFVADQSFWELFPGAKLGVVLLKDVQNQAESPEDVKQLLADS
                     HELAKAYLTADNFSDNQVVQVYRKAYQHFKTKKGARSSIEALLKRVSNGQSIPSINPL
                     VDIYNAASLRFGLPAGAEDSDSFIGDLRLTITDGGDDFYLIGDADNNPTLPNELCYKD
                     DIGAVCRCLNWRDGERTMVTEHTKNAFLIIEALDQEGQNRLQEALKFIEGSAKMYLHA
                     ITSVHVLDKDNPHVPLFTEN"
     gene            complement(48303..48776)
                     /locus_tag="ACGEUI_000540"
     CDS             complement(48303..48776)
                     /locus_tag="ACGEUI_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8165399.1"
                     /translation="MNKKETRHQLIRSLISETTIHTQQELQERLQKNGITITQATLSR
                     DMKELNLVKVTSGNDTHYEALAISQTRWEHRLRFYMEDALVMLKIVQHQIILKTLPGL
                     AQSFGSILDAMQIPEIVATVCGDDTCLIVCEDNEQAKACYETLSHYTPPFFFSNK"
     gene            48918..49598
                     /locus_tag="ACGEUI_000541"
     CDS             48918..49598
                     /locus_tag="ACGEUI_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8165400.1"
                     /translation="MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFF
                     EGDKRDKLFLVTSGYFKVEQSDQSGTFMYTDFIRHGTIFPYGGLFTDDYYHFSVVAMT
                     DVTYFYFPVDLFEDYSLENRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLA
                     ILLVEMGKDSDTLPFQLTTTDIAQISGTTRETVSHVLRDLKKQELITIKGKYLTYLDK
                     DYFLQYTK"
BASE COUNT        14885 a         9222 c        10801 g        14726 t
ORIGIN      
        1 atattattga aattatgtta acgttaaagc tttttagctt tgtaagtgca attccgcttc
       61 gcctgaaaaa tattttttat ttatcactta gtatggtttt gtttcaagta gttttttggg
      121 ctttctttcc agaccatttc attcttgatg ttgtgatgtt ggctcagttt cttttctttg
      181 cgcttatagc cctatactat ggtaaatcaa taaaagctaa gtttttaatg ttttatgcct
      241 tttttccact agtgtctatt agtttggtta agcgatttat tgtatttttt gttatgccat
      301 tgtttggtat gccttattca gttgttaaac ataatacact actgatttac agcattactt
      361 gttttagtat ttttttgatt tatcgctgca ttcaagtttt tcatttcgat ttttcaacct
      421 ggcgccaata ttttcaatca catagagcta gtaaactatt agtgtttact aactcttcga
      481 tggctctata ttacttgtgt gtccaaggga tagatgtgat gtcgccttct ttatcaggac
      541 ttgctacaac gactgctcgt tcaatcatag tgctctttta tttcattttg tttcttactt
      601 tattaattca tttagagcgt tatgtaaaac aaaactctat tgaggcaatt gtgcaacaaa
      661 aagaatatcg tgagctgatt aattatagtc agcaccttgg attgctgtat caagatattc
      721 aagagcttag gcggctatta actaccgtgt ctagtcgtct taagattgga atcgaacaaa
      781 atgatatctc tattgtgaga cttacttatg aaggtatctt gaatgctgaa aagaataatg
      841 ctaaagatga cagacttgat ttaacttgtt tagataaatt acaagttgaa gcaatcagac
      901 atattgtttt agctaaatta attgaggcaa aaaataagaa gcttaaggtt gaggtatcga
      961 tccctaattg tattgcaacg ttttttctag aagtagtaga tttcactaag ctattgtcat
     1021 ttttgttaga taatgctata gaaatgagtt tggagacaaa gcaaccttgc ttatcaatag
     1081 catttctgga tcaaaaccat aaacttgtca tagtcattca aagcagtact aaacaaggac
     1141 aagatgatag tcaaagcgtg tttgctatac cggctttgaa aaaaagagat gactggcaat
     1201 ttgacttaag gaatgtcacg accattttaa atcgttatga ctatctcaca attagctcgc
     1261 agattcatga tggcatttta acccagttaa tagaaatagc taagcctgac tgatgttaaa
     1321 ggtatgaata atcactcaaa atcaacttaa gagtctgaga caatagtccc agactctttt
     1381 tgaatatgtt aatctattac taatgataat agtttattgt ttcttccaag ctagagttaa
     1441 aatttccgct ttgccaagtt ggctgggtgt gctttgcttg ctgtcttttt ctaacaaatt
     1501 agtgaccttg gcattgtagt ttggcaggct taggctaaag tcacaagttt tatcgtttga
     1561 gagattatag ctgcgcgtga taagggcatt gtctgctaag cgacgtttga aagctgtgag
     1621 ggcaacttgg tcgtttgtac ccgtcaaata gctatattct gcggctaatg ttccctcgtg
     1681 ttggtttgtt tgtgtggtaa taacagggac ttggccttct tgagctcgcc agtagctggc
     1741 aaattgtgtt tgcttagtga tgctttcaaa actataagaa aggctgtgtt tgccaagaca
     1801 ctgggcttca ggtgttggga agtaacccca gtcgcccatt tcgccaacag aacgtaagag
     1861 agtgatggca atggtgttgg tatctggtaa gatctcatat tcgttgaggc catagttacc
     1921 aatagtgact ccattttcac catcaaagag actcacaaag cattcttggt gctgtgggtt
     1981 acttggattc ttccaaaagg tggcatctgg atgatttgga cgtttgacag tttcaaaaat
     2041 actgtcagct agatgatggt ctgttttaag gtgcgttggg aataggacgc gcaagcgatg
     2101 attagtcatt tggttatcaa aacgtgtggt gaattggagg cgaggattat ttttctccat
     2161 gcggataagg gttgttaagg ttagctcagc cttttcttgt gaacgtcttg cttggcgttc
     2221 tgtgatgtca atgacagcct ccatctcagc ctgtaagagc ttatctgcgg agataggaat
     2281 ggcaaaggtt tgctggattt caagttcagc aacttgagcg gtgttgctaa tgatgttaag
     2341 cttgatggtc ccttgatccg catagaaagg ttggtcatga tttggctggc gagaaatata
     2401 ttcatttcca atatcgccac aatcttcaaa ccgcaacagg tcttgataga ttagtccaga
     2461 ttgtttatcg gtgatggtta gacgaccatc ggtttgtatc ataactttta gaaacccatt
     2521 ttcaaggcac tgattactgt catcgtagag ggaaacggtt tctgaaggag ttgtttcatt
     2581 tcctagcttt aatgctaagg ttttccaaga catggctggg agagttattg gtagccgtaa
     2641 tctcactttg atggcgaaat aaggttcgcg gaaggatctc ttgggcaaat cgtagtcaaa
     2701 agcgatgctt gtgcctaaaa tttctgcttc gggtctcact tgtccactag tgtcaataat
     2761 ttggaaagat tgggagagtc ttgccaaata ttcttgagct tcttggtaaa cctctttagg
     2821 aaagcgttgg ccaaaatgat attttttcca ggtcaggctg agctcagcaa cacttgtttt
     2881 ggaatggccg ctggtattaa ataagacgaa gggttggcta tccattggaa aatccctggt
     2941 atcaatggcg tcagcaattt gcttagcagc ttcttttgct aaatagtgtc cgacttcata
     3001 ggctttttca aagcgcgtca tcatttcccg atgaacgcta tcaacactac aaccacagat
     3061 agaatcatga gggtgatttt gcatgagggt tttccaagcg taacgcagtt ggtcgtgagg
     3121 gtaggtactt gttacctcat aagccattgc tgctaagggt tcggtgatgt tttcgagttg
     3181 gcgagagact ctggtattag cctgtttgag gtaaatacga gcagaagccg tgttagctag
     3241 ggtataccag ccatcggttt cttgactggt aatctctcct tggacggttg aaaggttctc
     3301 tggtaaatca tctgcgagat cagccaagta atcttcaaag caggaatgca caaattcgta
     3361 gtcaggatag agttggttgg ctaaggcgat tgccttggtg acatcaagtt gtacgggttg
     3421 atgatcacac ccgttcatca tcagaaggtg cttggttgag gcgaagcgtt cggcatcagc
     3481 aagtttttta tcccaaaaaa gacgcgcctc agcttctgtt gtcgggatct cattgccatt
     3541 gctgtaccag ttggcgaaga ggagtccaag aatacgactg ttatctgggc cttgccaact
     3601 gatttcagag aattgggagc tgtatttttc actggtatcc acctgattgt taaatccagt
     3661 tggacgaatg ccacgaccaa aggcagcagc ttgtaggccg gcttttaaca tcagctgtgg
     3721 tgtttgtccc atatttccaa aggtatcagg aaaataacca agtggcacac tagcgcccca
     3781 tctgtcacaa tcttccttac caatcagcat attgcgcaca ttggattcac tgctggtcaa
     3841 aaaatcgtcc tgtaagatat agaaaggtcc gatacggagt tttcccgaag caatggcttg
     3901 tctaatctca ggttctcgtt cggggcgtac ttttaaataa tcatctagga taatggtttg
     3961 cccatccaaa tgaaaactat gaaaatcagg atccgtttga aaaacttcta acaggtcatc
     4021 tattaagtta atcagacgca tgtggtgttg ttcgtaagcc atgtaccact cgcgatccca
     4081 gtgactgtgt gaaataatat gtactttttt agttgccata gtggcctcac tttctaagga
     4141 gcaatactaa agcccagata gtcaagaagt agttcacaga acatcatgtt agcccaagag
     4201 aaccactcac gagtgtattg ataaggatca ttgacatcga aaccctcgtg cattaagtgg
     4261 gtaccagcat ctgttgccac aagcaaatca agcatctctt ttttactgtc ttggtcaaga
     4321 gcggtcaatc cttgtaaggc aagggcgata tgccagatat aattttctgg tgtatgggaa
     4381 gaaccgatgc ctgctgctgc atttccctga taataataag ggttttcttg gctgagaatg
     4441 gtgcggcgcg ttgccagata gatagggtca tctttagtgc aaaaaccaag gtaaggtgca
     4501 gcaaggaggc ttggaatatt agcatcatcc atgatggaaa actgtcccag accatccact
     4561 tcataagcgt aaatggtttc tcccttgctg ttttgaacaa gggcatggtc tgcaataccc
     4621 gattgaatag cttctttcag ctgcttggca cgagtagcta ctggttcatt gtgaaaaagg
     4681 ttgttatata aatcttctag ataggaaagc acaaccactg caaacatatt tgaaggaatg
     4741 aggtaaccat attgacaagc atcatcactt ggtctaaagc ctgaccaggt catccctgtt
     4801 ggtgcgacag gacttccttt tccagcatgt gttagggtat cttctttgcg ccaagtatcg
     4861 cgttcaaaca agtaagggga ctgggcgtgg tcttgctcaa ctgtccatag gtcaagaatg
     4921 agttctaccc cacgatgaaa atcgtcatta aactgatcgg tgcttcctgt ttcacgatag
     4981 agcagataag ccagttgaat agggtagcaa aggcaatcaa tctcatattt gcgttcccaa
     5041 atccaagggt tcatttgggt atgatctgtt tggtgtcctt tttcgttagc ttcttcgttg
     5101 aaggcattgg catagggatc gatacaaatg tagcggaatt ggcgtttgac cagaccggca
     5161 ataatctttc taatttcttc atcttccttg gcgaggaaga ggtaaggttt catttgcgca
     5221 gtggaatcac gtaaccacat ggcaggaata tcgccagtta aaagaaagct agtgccatct
     5281 tcgtaggttt ttagggttgt ctctaaggta ttggtaaagc tggttttgaa attaagagcc
     5341 caatcagggt gttggtctcc acaaaggctt tccaccttgg ccataaattg gctgacgcta
     5401 gttgggattg gtcgtgtcat gtcatatcct ccaaaatgat atatcattta tcttcggttt
     5461 cattataaat gataagttaa actttgtcta taggtttttg aaaaggttta caactctttt
     5521 ttgacatgtt atactgataa caggagtaaa gaatgacaaa attattatac aaaaagatat
     5581 atcatttcat taaagagcag atagcatcag gtcgtttgca aattggtgac cgcctaccta
     5641 ctgaaaaaga attgtcagaa cagttctcgg ttagccgtat cacttctaag cgagcgcttg
     5701 tagaactgga gcaagaaggc ttgattacga gaagtcgtgg gaaaggaagc ttcgtagcta
     5761 aaaagcaagt gacgtcccct gaagcgaata aggatttatt gttgattctt ccttttgctt
     5821 ctgattatga attgggagat tatgctaagg gaattatgac ctccattgct gaaacaggtt
     5881 accgtctcat ggttcaattg gccagtacag taaggttaga caccctgtca gactatgctg
     5941 ggattattta ttatcctgaa gatgtcaatc acagtattga tttcctgttt tactgcgacc
     6001 atcatcacat tcctctagtc ttgttggata aatctctaga tctttttcag tttccgtctg
     6061 ttgtagcgga taataaaggc ggtgcctgtc aattgactca gcacttgatt gagcaaggct
     6121 gtgaccagat tagttttgtg gcaacaggct cttttggaga agtatcatcc gttagagacc
     6181 gttatttggg ttacttagcg gcaatggcag aatcgtctct aaaatcccat tattttccta
     6241 agacagagac tgaaacaaca gatgcttatt tggaccgttt ggtgaaaacc ttatcggaga
     6301 cgacagccac taaaaggggg cttgtcgtgg aaaacgattg gttggctatt cagctgattc
     6361 aaaaaatcac acaggtaggc ttatctattc ctgatcaagt cgctattgta ggctttgata
     6421 atagtcaagc ctcccgtttg ttgagtccca aattaagcac agcagctcaa gatttttacc
     6481 aaatggggca agaagcagct aaagtcttgt tgcagattat tcatgagcca gaccaagcag
     6541 tggtgtcttg ccagttacct gttcaagtat ttatcagaga aagtagtcac taaaaaagac
     6601 gttactagca tgagctggtt agctcaggta aaggagacca gaaatgacca tttatcacgc
     6661 cttaaaggat tatcaagaag tcatgacaag aggagactat cttgtctttg acacgccatt
     6721 gacttgccga tttattggcc gttttttccg ctttgaaaat caaaaagccc tgctggcaga
     6781 attggcaacg tcaaagtatt ttcagtggat tgaagaaggc caagcagagg tgacgatgaa
     6841 acactttttt aaccgtcagc tggctaagga tgcctttacc ctgaagattt cagaagataa
     6901 ggaaatcatt attgaaagcc aaaaccttag aggttttcgt tacgcccaag aagctttgct
     6961 taaagtcatg accttcaaag gggataaact ttatttgccc attgtttctg tcaagcacag
     7021 cccttctttt gctatgcggg ggataattga aggcttttac ggaacaccgt ggacacgaga
     7081 agagcggtta gattgcctgc gttttattgg aaataaaagg atgaatactt acatgtatgc
     7141 tcctaaagat gatgattacc aacgaaaact gtggcgcgat ctttatcctg aggattgggt
     7201 gacttatttc aaagagctac ttgcagtagc caaggaagaa gggcttgatt tttggtatat
     7261 gattagtccg gggcttgatt ttgattatac caaggaagct gactatcagt tgctctatca
     7321 aaaattgcaa caacttctag cgttaggtgt ttgtcatttt gggcttttgc tggatgatat
     7381 tgactaccaa attgtagatg ctgttgagag acgattcaaa aagactgcct atgcccaggc
     7441 ccacttggca actgaggttc atcacttttt aaaccaacaa catgcggcgc cagaattggt
     7501 gatctgtccg acagaatacg acaaccacca tgattctatc tatttgcaag agctgtcaga
     7561 aaggattcct aaagaagtcg cctttttctg gacaggccca agcacgctag cgagtcaaat
     7621 ctcgcaagca gatattgaaa ctatggcagc agtatatcaa cggcctatca ttatttggga
     7681 caatattcct gttaatgatt atcaaaaaga tccagaacgt ctctttttaa ccccttttgc
     7741 caatcgctcg ccatttttat gccagccagg ctatcaggtc aaggggattg tttctaaccc
     7801 tatgattagt tgggaattat ctaagcttac cttgacagat atgagccatt atctgtggga
     7861 tgctaaccgt tatcagccta gtcattcttg gctagaaacc ttaacagact atacagaaga
     7921 tactgaatta gccttagctt tacaggcatt tgcttggcat aatggcaatc gtcatctgca
     7981 cagggacctt ccttttgaag tggaagaagc tctcctggct aaggatgtat caacgttatc
     8041 agcttgggta gctgaattgg tcgaacgtgt caatacgttg agaaagctag ataagccagc
     8101 gtttcaacaa gctattgctc cttggtttga acgggtggcc aaagaccaag atttttggca
     8161 ggctatgtta aatcaggagc ctcagttgga aacactctat gctgacttac aagaagataa
     8221 acaccgtatt ggaagtgaca ttccaagtcg ttactatcgc atctactatc aacagcaaga
     8281 taaactaaca gctaatcaag gtcaagttac ccaagcaaga ccggaagact atgcttagca
     8341 tacgagctag aaaggaatga tatgttagca tttccaaaag agttttggtg gggaggagcc
     8401 acctcaggcc ctcaaagtga ggggcgtttt gctaagcagc accgcaatct ctttgattat
     8461 tggtatgaag aggagcctga cttgttttac gattacgtgg gacctgatac ggcttcggat
     8521 gcctatcatc agattgaatc tgatttgacc ttgttagctt cactaggtca caattcttac
     8581 cgtacctcca ttcagtggac acgtttgatt gatgactttg agcaagcgac gattaatcct
     8641 gatggccttg cctattataa tcgagtgatt gatgcttgct tggccaatgg tattcgtcct
     8701 gttattaatt tgcatcattt tgacctgcca attgccctct atcaagccta tggtggctgg
     8761 gaatcaaagc atgttgtgga cttatttgtc gcctttagca aggtttgttt tgagcagttt
     8821 ggtgatcggg ttaaggattg gtttgtccac aatgaaccta tggtcgttgt ggaaggctct
     8881 tatttaatgc aatttcatta ccctgctatc gtggatggga aaaaagcagt ccaggtcgcc
     8941 tataatctag ccctagccac agccaaggtt attcaagctt acaggcgagg tccagcagaa
     9001 cttagtgatg ggcgtattgg aaccattctc aatctgacgc cagcctaccc agctagtcaa
     9061 tcagaagcgg atatggctgc tgcgcatttt gcagaacttt ggaacaatga cttgtttatg
     9121 gaagctgctg ttcatgggaa attcccagaa gaattggttg cggtattgaa aaaagatggg
     9181 gttttgtggc aatcaacgcc agaagagttg gctcttattg ctgagaatag agtggattat
     9241 ttgggattga atttttacca ccctaagcgc gtgaaggctc ctgatgccat tccagtgatt
     9301 agcccatctt ggagtcctga atggtattat gatccctacc tgatgcctgg ccgccgcatg
     9361 aacgtggata aggggtggga gatctatcct gaggctgtct atgatattgc catcaagatg
     9421 cgagaccatt acgacaatat tccttggttc ttatctgaaa atggtgtagg gatttcagga
     9481 gaagaccgtt accgtgatga aactggtcag attcaagatg attatcgcat ccaatttttg
     9541 aaagaacatt taacctatct ccacaaagga atagaagcag gctcgaattg ttttggttac
     9601 catgtatgga caccaattga tggctggtct tggctcaatg cctacaaaaa ccgctatggc
     9661 cttgtggaaa ataatatcca tacccaagtg cgacgaccta aagcttcagc ttattggttt
     9721 aaaaaagtag ctactcataa ccgcttgatt tcattagaag taatggagga gtttggaggc
     9781 tctgcttctc acctcagtga ttgaacgtgt gaacgtctcc tttgagacaa gtgaataatg
     9841 atgttaccaa tgattaaaca agggcctttt tcctcatgtt aagaggatcg aggctcttgt
     9901 atttctttgg aaagtgttgg tatttgccga actagatata agcgttctgt ctggtttagc
     9961 atcaactacc catcttgttt aatttgactc ttcaattctt tagagtatct tggctaagga
    10021 tgcttgtttt tcatcttggt attttaggat tttctttcat atttttgggc cagataaaag
    10081 tcccttatgg tttctagtga ctgtttgtta tgtcactgtc cacgataagg ggactatatg
    10141 agtgagttat taagtgaggc tttgcttatt ggcgataact agcatagctt ctaatagctc
    10201 gagcattaac tgatgaccaa ttcctttaac agaagcgtca gcaacaatga catcaacaaa
    10261 gggaccaggc atcaggctgt gttcatcagc agagagtcat ttaggcagaa agccagtatc
    10321 gttgtagcta atcagcaatc cttttaggat gtctttgtag atgtcagagg cgattaggga
    10381 caatagggga taagagggat ttagcggtcc atggttaggc ctccttgtgt tcttggtaaa
    10441 aatcaatggc agctcccaga agattagctt cgtttttaaa gtgacaaata gctaattggg
    10501 gtctaaggtc ggagatttcg acggcttggt agagtttgtc tagttcagct tcaatcgctg
    10561 acagtagaaa gtcagcttgg gaaacaccac ccccgattaa aatcagttga ggatcaaagg
    10621 cgtgttgaat gttaaaaata ccgatggcta aactttggag aaagacctgc ctttctttga
    10681 gtgctagggg atctccttgt tctgcgaggg caaggacagc tttgccagta taatcttttc
    10741 cgttattgac gattgcacta tagcgcttgg ccatatttac cactgtccct aactgactaa
    10801 aggtttggta gcggtcgttc atgatcatga aaccaaactc gcccccaaat aagtgggcac
    10861 catggtaaat cttgccatca atgaccaagc ttcctccaac tccagtacca agaacgagca
    10921 tggctaggct actagcgcct tttcctgccc ctaaggtagc ttcagctaag gcggcacaat
    10981 tggcatcatt ttcgatagaa atagggtaat gtagccgttc ttctaaggcc tcttgtattt
    11041 taaaatggtg aatgtaggga atagcgctgg cgccttcgat aacccccttt tctttattga
    11101 ctgccccagg tgaactgatg gcaatacctg agaaatggta agcagagtaa cgagcaactt
    11161 cttggtcgag taattggtaa aacttttcca gactagaggg agtgggaaag ctgctttgct
    11221 ggcttaattg gccattgtga cagagggcaa atttaaggga agtgcctcct atatcaatac
    11281 ataacaagct catgaggtgt tctcctttat tatagtagaa tagacgatca tgtctcatct
    11341 tagtatgaca gatttaagca acaacgaaaa ggctttctaa gtgattctat actttgtgtt
    11401 atcataccta taatttgttg ctttcaaata atgaaagcgc ttgatatact gtaactataa
    11461 cgtgattaac acaaagggag gttgggaaat gataagacaa accaacaaaa agacatcctt
    11521 ttggaaaaat gtgattaaat accgggcctt attattaatg gtattaccag gtttcatctg
    11581 gttcatcttt ttcttttata ttccagtttt agccaatgtg gttgctttta aagattttca
    11641 ttattcagca gggggcttta tggaaagttt gaaggagagc ccttgggttg gtttggctaa
    11701 cttcaaatac ctctttgctt ccaaagatgc ttggttgatt accagaaata ccattgctta
    11761 caatgtgatt ttcttactct ttaatgtttt ctttgcgatt gcttttgcca ttatcatgag
    11821 tgagttacga aataagagaa cggttaaggt ctatcacacc atgtccttat tgccttattt
    11881 cttatcatgg gtggttattg aatactttgt ttctgccttt ttaaatacgg ataaaggttt
    11941 cattaatcag ctcttgaccg gaagtggagc agatccaatc aagtggtatt ccaatcccac
    12001 ttggtggccc ttgattttac ttttcatgag tgtctggaag gggctaggct acaatagcat
    12061 tatctactat gcttcagtca aagggatttc tgatacttat tatgaagctg ctatggtgga
    12121 tggtgcaagc aaatggcaac aaattaggaa catcaccatt ccacaattat tgccaatgat
    12181 gtctatctta ttgatcatta atattggtaa tatttttaaa tctgatttcg gacttttcta
    12241 cgttattcct aagaattcag gcccacttta tgacgtgacc agtgtgttag atacctatgt
    12301 ctacaatgcc ttgactgcta caggagatat tgggatggca tcggcagcta gcctttatca
    12361 gtcagtagtt gggaccagca tcttgttagt caccaatgcg attgttcgtc gcatggaccc
    12421 tgatgcagcc ttgttttagg aggaaaacat ggagaaaaag aaaaaagtcg aaaaggttaa
    12481 tgttagaacc tttgatcgaa aaaccaatgc tatctttaat attctgattg gtctgtttgc
    12541 catctcttgt atcattcctt ttatcttcgt gattattatt tccttcactg atgaaagtta
    12601 cttgattaat catgggtata gtttttttcc agatgtctgg tcaactaagg cttaccagta
    12661 tatttttcaa ggagccatgt cccatagaat catgaggtca tttgggatat ccgtgtttat
    12721 tacagtggtg ggaaccttta ttaacacgac catgacatca acctatgctt atgcgatttc
    12781 aagaccttat ttcccataca gacgtttttt tactgtttat gcacttatca ccatgctctt
    12841 tgcaccaggg atggttgcta attacttggt ggtcagcaat ctccttcatt tgaaggatac
    12901 ggtttgggcc ttgattttac caatggctct gggcccattt ggcatcttgg tcatgagaac
    12961 gttctttaaa aagacagttc cagatagcat tattgagtcg gctcgtatgg atggggctag
    13021 tgaatggatg atttttatga aaattgtctt accattagct gttccaggga ttgccaccat
    13081 cagtttattt tctgccttaa cttattggaa tgattggttt aacgccttgc tttatgtgca
    13141 aagtgagaat ctttacccaa tgcagtactt actgatgaaa attcagagca acctacaggc
    13201 cttggcacaa aatgctggca tgagtgcaca aatggcagat agcttagcat cgctgccaaa
    13261 agaatcagtt cgtatggcta tcgtggttat tgcaaccttg ccgattgcct tgacttaccc
    13321 attcttccaa aagtactttg tcggtggttt gaccattggt ggggtgaagg aataggactt
    13381 aaggaactgg tcacaagacc agattttatc taatcattag taacaaaaga aaacattttt
    13441 taggaggctt cacttatgaa aaagtggcaa aaaatcgtgt gtgtgactgg aactgtgctt
    13501 gcagcgtcta gtttagcagc ctgcgaaagc aagtcagcat caaaggatag tgatgtcaaa
    13561 ttattgatgt accaagttgg tgacaaacct gataacttcg atgaattgat gacaattgct
    13621 aacaagcgca tcaaagaaaa aacaggtgca acggttgacc ttcaatacat cggttggggg
    13681 gactgggatg ataaaatgag taccatcatt gcctctggtg aaaactacga cattgctttt
    13741 gccaataatt atgtggtcaa tgcacaaaaa ggtgcttttg ctgatttgac aacgttaatg
    13801 ccaaaatacg ctaagaaaac gtataaaaac ttagacccag cctatattaa aggaaatact
    13861 attgacggta aactctatgc cttcccagta gatgccaacg tttatgccca acagatgctt
    13921 tctttcaata aagaactagt ggacaaatat ggccttgaca tttcaaacat taagtcctat
    13981 gcagatgctg aaaatgtctt gaaacaattc cacgaaaaag aaccaaatac agcagctttt
    14041 gctattggtc aagtctttag tatgtcaggt gactatgact acccattaac caaaacccaa
    14101 ccctttgctg tgaaaattga tgaaggcaag ccaaccatca ttaatcagta tgaagatgag
    14161 tcctttaaaa acaatctccg cttgatgcac aaatggtata aagaaggttt gattccaaca
    14221 gatgcagcga ccaatacaga aggttatccc cttgaaggaa acacttggtt tatgcgtgaa
    14281 gaaacccaag gtcctatgga ctatggcgat actatcttga ccaatgctgc aggaaaagac
    14341 atcgtgtctc gtccattgac taaaccgcta aaaaccacat cacaagcaca aatggcaaac
    14401 tttgtggtat caagcgtatc taaaaacaaa gaaaaagcag ttgaagtcct ttctcttctt
    14461 aatagcgatc ccgaattgtt aaatgggctt gtttatggtg tcgaaggcaa agcttgggaa
    14521 aaaattggcg acaagaaaat caaactgctc gatggctatc aaccaaaaat gcacatgggt
    14581 gcttggaata ctggtaacaa taaaatcctt tacactcaag aatctgttac tgacgacatg
    14641 attgctaaac gtgaccaatc aatcaaggat gctaaagaat ctccaatcct tggtttcact
    14701 gttgacacaa aagttatcaa aacggaattg tcaaatattt caaatgtcat gaaccgatat
    14761 aaagcaagta tcaatactgg tacagtggat cctgacgaag cccttccaaa acttcttgca
    14821 gacctcaaag gtgctggttg ggacaaggtg caaaaagaag ttcaaaaaca attggacgac
    14881 tttgtagcta aagacaagta aggcattcat tttcttgatt ataagttgag ggctgggaaa
    14941 tctcagctct tttcttctga tgagtggaaa aaacggatca tcaaataagt ggaagttata
    15001 aagggagaaa aattaatgtc aataatgtta ctactcttag taaagcggtt tccaaaagcc
    15061 tataaagtgt gctatattag aagagtctaa actattaatc agagtaaaac taataccagc
    15121 atcttactgg gggtgctaaa actagtctcg tagctgctgg tccctgaaac tgctataagt
    15181 gttaacaaga aaaagaggac tatcatatga atggcattga aaaaacctgc tttttaattt
    15241 ggaaaatgat ccaattgacc ctcatttttc atctcttgtc cttagctgga ctgattgtgt
    15301 ttggggtggg gccagcctgg caaaccattg tcaccctatt tttaaaaaca cctcaagaag
    15361 aaaaccacta ttctttgaaa agggctgttc aactttggaa atcttatttc aaagaagcta
    15421 atctaagatt tggtcttttt gccctgactt tccttttttt aacctttaat ttgcattggg
    15481 cagtacagtt tccatccttg ttttggttta ccgtgtcttt cttgattgta atagccatgg
    15541 tttggctaac catgacctat ctttacatgg ttttttatgc agtgagctat gagattaacc
    15601 tttggaataa tatgaaatta gcctttatca gcgtcttttt aagtttcaaa tcctttttac
    15661 tcatgctgag tgttttgttg ggaattacct tagtgacttg gcaatacaaa ggtctttatc
    15721 tctttttaac cttcggagct ctagtctttt gcttggactt tgtcactaag gctaatcgtc
    15781 gactagtaga tggagtgatt gatgagtgtt aaaacattgg gaaggtatgt gactaaccac
    15841 tttaagcagg gttttttgat taatcgactg atgaaattat atagcctgct aattgtaagt
    15901 tttttcactt tagcagctat tggcttgagc agttattcta ttagtagtac ttataaaagg
    15961 gtggatgctg aggcgcagat gcgattggag gagactctag gtagattgca gtcacaaaac
    16021 gatattaccc tacgggtttt agaccagttg gttggccgtt cagaagatta ccataatctc
    16081 tatcaataca tgacgttaac cccaaaccaa tatttttcaa acgtttttga agactgggaa
    16141 aaaggaaaag acacggtcct attttctgaa gaggtgaggc gtctttttga cttgtaccct
    16201 gatgtgactt ctatgaccct actgctagag gacagtagtg cttatctgta cgccgataag
    16261 gtcattaaga cgggtcgttt gctttatagc aagcctgaaa aagtaaccgg aaatgtttta
    16321 gttcgctcta ttcgaaatcc agaatcagga gatgtgacag gtcgtcttta cctgaccttt
    16381 gacaaaccac cgactttggt agagacacag caggatcact atttagctac ttttgctttt
    16441 gattactatg gccgcaaact cttccatcaa ggaggtcgga gatttcctca tttagaggct
    16501 gaggttaaaa aggctatcaa ggcagatcgg gcagctgatc ttagtaatct tagcaaggct
    16561 tatcggatgc agtacaatcg ctcgggagac ctcttggctt atgtggctgt cagaaaatcc
    16621 tatttattag cggaagctgt caggacagtt tttgtgtatg gccttgtttc tctactttta
    16681 gcttggctgt tattgcagtt gctctttagg gttttccgaa attatatcca gcaggtttct
    16741 gaaattacgg atactgttga aatggttgca gcaggcgatt tgtctttaac catcgacaac
    16801 agccacatgg aattggaact gtatcatatc tcagaagcca ttaatcagat gttggccagc
    16861 attaaagctt atattgacga agtttatgtg ttagaggtag agcaacgaga tgcccaaatg
    16921 agagctctgc agtctcaaat caaccctcat tttttataca acacgttaga gtatattcgg
    16981 atgtacgccc ttagttgtca acaagaagaa ttagcagatg tcatttatgc ctttgcgagt
    17041 ctgcttcgca acaatattag ccaagataag atgaccacct taaaagaaga actggctttt
    17101 tgtgaaaagt acatttacct ttatcaaatg cggtatccag atagcttcgc ttatcatgta
    17161 aaaattgatg agagtgttgc tgacttagcc attcctaaat ttgtcattca acctctcgtt
    17221 gaaaattatt ttgtgcatgg gattgattat agtcgccatg acaatgcact aagcatcaag
    17281 gctttagatg agacggatta tctcttgatt caggtgcttg ataatggacg tggtattagt
    17341 caagagcgct tagcagatat ggaaaaaagg cttcaagagc accaaacaac aggcaatagc
    17401 tctattggtt tgcaaaatgt ttacctccgt ctctttcatc attttcgaga cagggtttct
    17461 tggtccatgg ctaaggagcc aaatggtggc tttatcattc aaattaggat tagaaaggat
    17521 gcttgatgta taaggtacta ttagttgacg atgaatacat gattttacaa gggctcacca
    17581 tgattattga ttggcaggca cttggatttg aagtggttca aacagcaaga tcaggcaagg
    17641 aagccttagc ctatttgact cagtacccag tggatgtcat gatttcagat gttaccatgc
    17701 ctggaatgac aggactggat ttaatagaag cagctaaaac ctatcatcct cagctacaaa
    17761 ccttgatttt atctggttat caagaattct cctatgtgca aaaggctatg gaattagaaa
    17821 ccaaggggta tttgttaaaa cctgttgata aggcagaatt acaggctaaa atgaaacaat
    17881 ttaaagactg gttggatgcc cagcaggcag aatcaatccg gcaagaagcc tatcatgaca
    17941 gccttttgac cctttggctt acagatgaat tgagtgagaa agaatttcag cagttaagtc
    18001 aaggcttgcc agcggctgct ttaactggtt ttacagtgct gtatttggac tgtcaagagt
    18061 ggcaagcagc catcgatgct tattttgaac gagaaggtca gcctttttat ctcaaaaaag
    18121 aagaagataa gaaggtttat ctggctgtcc tacttggtaa aactgatcaa gccaaggcgt
    18181 ttacctatga tctacaaaag cgattctcaa gaaacatgca tcaacttatt ttgggagaaa
    18241 cagttgatga ctgggaaaat gtttatgaaa gctacaatca ggtgcttaag agcctctttt
    18301 acaattgtga agtgtcacca attagagcaa aagataagtc caaaattgaa ctaccagagt
    18361 cccgtttgca gttctttgct tttaacaagg cgttgatgat tggggacgaa cctacaatct
    18421 tgtctaaatt agaagctatt tttgacgaga tgaagacctt gaacttttca ccagaggatg
    18481 tgaagcatgt ttcgttcttg ttgttttctg acatttatcg tcaatttccc attttagata
    18541 aaatgactta tctgtccatg gtaaaaacta ttcatgacag ccaatccatt gattgcattt
    18601 tgagggaact aaaaaaagtg ttggatgtca ccaatcagaa caattcccct gaaaaacgct
    18661 attcagattt agttagtgaa accattgatt gcattcgcaa ggaataccat caagaattaa
    18721 ctcttaaagc tatcgctgat aggttacatg tcaatggggt ttatttaggg caatgcttta
    18781 aaaatgaaac cgagcgtagt tttacccagt acttaaatca tgttaggatt caaaaagcgc
    18841 aacagttatt gctttacacc aaccaatcca tcaatgaaat tgcttatgaa acaggttaca
    18901 ataccaatca ttattttatc aagatgttta aaaagttgaa tggtttatcg ccaaaagagt
    18961 tcagagatcg ttacaaggac aattaccaag ccatcaaggg cgaccagtaa cgtcaaacag
    19021 gagattgata tgacaccaga tagcaaggag cagacagtga ctggctacca ataccactat
    19081 attgatcaag aaggaagaaa gcagcccttt aatcaaggct ggcgttttct tatggcagat
    19141 gtggcgtgtg ctcaggaccc ttcttttgat gattcgaact ggcaggtgat tcatttacca
    19201 catgatttca gtcttacgca gccttacaca agaaatggtg aagctgaaag tgcttataaa
    19261 ttaggtggtg tcggttggta tcggcattat ctcgtgttag atgaggtttt agctggttgt
    19321 catgttgcca ttacttttga gggttcctat atggaaacgg aaatttatgt taacgggcaa
    19381 ttcattggca aacacttaaa tggctatcag gaatttactt atgatatctc tgatgttgtg
    19441 acttttggag ctgaaaatct tctggctgtc cgggttgaaa acaaggttcc cagttctcgt
    19501 tggtattcag gcagtggtct ttatcgtgaa gtgagtttgt ctgttcttcc ccagcttcat
    19561 tttgtggcag accaggtagc aatgaccttg gctgatactg ctgtgcagga gaaagggcaa
    19621 cagaaagttg atttacgctt tgccctcaat cagtctattc agacttgcca ctaccaattg
    19681 agcctctgtt tatgggaaca atcacattgc tcaaaagaca agaaactcct ttatcaagaa
    19741 acagaagtgc ctctagctga tttggctttc caaagacagt atggtcttac actgtctttg
    19801 gaagaactcc aattgtggtc accagataat cctcacctct acgatttaga actcactctt
    19861 tactatcaag gtcaggtcat tgattgcttt tgccttgaaa ctggttttcg tcagctgact
    19921 tttatggcca atcaggggct ttttgtgaat ggcagagctg tcaaattaaa gggagtttgt
    19981 ttgcatcatg accaaggagg cctaggagct tgtgcttacg aagatgccct agccagacaa
    20041 ttagtcctgt tgaaagacat gggggctaat accattcgta gcacccataa cccaagtagt
    20101 cccaaactta ggcaactagc caatcgtctc ggcttttttg tgattgaaga agcctttgac
    20161 acctggacct atgccaaaaa tggaaacgtt aatgactttt caaactattt ccatcagact
    20221 atcggaactg aaaatgcaaa ctatcttcaa agagtgaggt cacctgaaac gagctgggcc
    20281 cagtatagta ttgaggccat ggtctggtct gccaagaatg acccgtcagt gttgatgtgg
    20341 tcgattggta atgaattgat ggagggtttt tcagccgatg tcagtcatta cccagagctg
    20401 acaagacaaa tgtgccaatg gattactgct attgacacct cacgtcccat tacctttggg
    20461 gacaataaat taaaagaagc tgacttttgc tggcatgagg aagtgtcgca aatggcaact
    20521 cttcttagtc aattagacca tcctcaggga ctcattgggt taaactatgc tgatggaaaa
    20581 gactatgata ggcttcatga ggagcactct gattggctgt tgtatggttc tgaaacagtc
    20641 tcagccatta ccagcagggc ttattataaa gagacaaaaa aagttcttga ctccggttat
    20701 cacctaacct cttatgatca tgctaaggtt gattggggtg ccttcgctag ccaggcttgg
    20761 tatgatacca ttacccgtga ttttgttgct ggcgaatgcg tttggacagg ctttgattat
    20821 ttaggagaac ctaccccctg gaacaagatc gacagcggtg ttgtaggcct ttggccttct
    20881 cctaaaaatg cctactttgg cattttagac acggcaggct ttccaaaaga tagctattat
    20941 ttttaccaaa gtcagtgggc gcaggggcag accaccttgc acctgttacc agtctggcag
    21001 aaggaccagc tttgtttcga tgagcaggga ttggtggagg ttgtggttta tagcaatgct
    21061 gccagtgtgc aactgatgtt tgaagatgag cagggcaact taacagatta tggaaggaag
    21121 gcatttcata cttatagcac gccaacggga cacacttatc agctttacca aggagctgac
    21181 gctgctaaga atcctcacga gaacctttac ctgacttggc gagtgcccta tcaaaaaggg
    21241 ttgctaaggg cagtggccta cgacatttct ggtaaaagca ttccaaaaac cagtggacgt
    21301 tctcaggtta gaacctatgg ttcagtggca aagctatctt ggaaagcctt tgaagcacct
    21361 attgacgctc cttgggaatt gctttatctg gatttatcac tcttggatag ccgaggtgaa
    21421 ttggtcagcc atgctcaaga ttggttgcag gttcaagtag aaggaccagc ccgtttgttg
    21481 gctcttgata atgggaatcc gactgaccac accccttatc aggagcctct gcgccaggca
    21541 tatgggggaa aattattagc cattttagcc ctcactggag aggcgggaca cattaaggtc
    21601 acggcaaaag cctcgcagca acttagcagt gtttttcaga cacaagtagc ctgccgtgcc
    21661 cccaaagaac acttagtgac actaccttta gagaaatatc agtttaaaaa gagccgactg
    21721 acaagtcaac aagaaattgg tcaatcttgt ctaaagacca cacaattcct ttctgttttg
    21781 gagaacaaag acacccagca aaaagcaggt aaggctagca cacctcacat tcttatcact
    21841 agttaccagc actatttatc tctgcccttg caggtttgtt ctcaggcatt tgattttaaa
    21901 aaaggcgacg atatcttctt gcctcagtgg ctccaagtca aagatgccca aggttctctt
    21961 gtgaaaaaag ctttttcaat gacgtggtac ctaaaagaga aaatctttgc caagcctatt
    22021 cttgtcacag gctatgtttc ttgttttggg caatcaatac cggttgaggc aaggcttaac
    22081 ctagcacaag ctagcaaaag acggcaccag gatctcagtt tagtcgccaa gcaatccctg
    22141 gccgcaagtc aaaaaaggct aacttattgc taccaatatg atacggtgca agccatagga
    22201 gccattagct tgcaaaatca cacaggcaca tctcttagtg tttctgtggc aatcagttat
    22261 ggtagtcaga gctctattaa gcaagaaacc ttgatgctag ctgatagcca cttgagccag
    22321 ttagaagagg ttattttctt cgatgatatt ttgactgttt tgtatctggc agtgaccctt
    22381 gaagtcattg aaggacctga cctaactgaa gacagtattc aaattggcct ttgggattta
    22441 gcatgaagca tgagtaaaag aagacttctt tggcaccagg tctgatgaat tctgtaaaat
    22501 aaggtataat aaaaaacaaa taggcgtcct ttattacgtg aaaggcgcct ttacaaggtt
    22561 gaaatggaaa gtgagatttc taaatgtcag aacgtttatc aggccacacc ctgttggtct
    22621 ctttgcttgc tacccctatt cgtcatagtt tgtctcccaa aatgcacaat gaagcctatg
    22681 ctaaactggg gctggattat gcctaccttg cttttgaagt agggacagag caattagcag
    22741 atgccgtgca agggattcgt gccttaggaa ttcgtggctc caatgtgtct atgccaaata
    22801 aagaagctat tttaccctta ttggatgact tgtccccagc tgctgagttg gtcggtgcag
    22861 tcaatacggt tgtcaataag gatggtaaag gacatctcgt ggggcatatt acagacggta
    22921 taggagcgct cagagccttg gctgacgaag gagtgtcggt aaagaacaaa atcattaccc
    22981 tagcaggagt tggtggggct gggaaagcta tcgctgtcca gctcgctttt gacggcgcca
    23041 aagaggttcg tttgtttaac cgacaagcta cacgtctaag tagcgttcaa aagcttgtga
    23101 caaaactgaa ccagctaaca aggactaaag taacccttca agacttggaa gatcagactg
    23161 cctttaagga ggccatcagg gaatctcatc tatttattga tgctacaagt gtaggaatga
    23221 agcctttaga aaatctaagt ttgattaccg accccgagct cattcgccca gacttggtgg
    23281 tctttgacat cgtgtacagc ccagcagaaa ccaagttact agcctttgcc cgtcaacatg
    23341 gcgcccaaaa agtcattaat gggctaggaa tggtgcttta tcaaggagca gaagctttta
    23401 aattaatcac tggtcaagac atgccagttg acgctattaa acctctttta ggtgatgagt
    23461 aacacttttt aagtaagctt aattatgata tattaataag agatggagca gtcatcatgg
    23521 gctgctaaaa ttctttttgc gagagctgtt tttaagagca aaaagcttat ttgaagagcc
    23581 ttatccctaa cgtgatacaa gtcatagttc ctttgaggtc tgccagataa cctaccgttt
    23641 ttttagaaaa gaagctctta taaaaggaaa agttcaatga ataaaggaga atagcaatgg
    23701 taacaaagga agttgggcat gactttttag cacgtctagg aaaaaaacgc ttaagacctg
    23761 gcggtgttga ggcgacaaac tggttgattg aacaaggaca attctctcag gattgtcacg
    23821 ttttagaggt tgcctgcaat cgctgcacga cagcaataga gttagctaaa accttcggtt
    23881 gtcagattga tgccgttgac ttaaacccca aggttgtcaa agaagcacaa gaaagggttg
    23941 ctgttgaggg gttaacccgt cacattacag tatcccaagc cagtgcttta tcactaccat
    24001 ttccagataa ccattttgat attgtgatta atgaagccat gttaaccatg ttgaacgccc
    24061 aagccaagga gaaggcactc aatgaatatt ggcgtgtgtt aaaaccaggc gggcgacttt
    24121 tgacacatga cgtagcttat gaagatccaa aaacaagaca agtacttgac gagttacgtc
    24181 aaacgattca tgtcaatgtt gctcccttgt cactaacagg ttggcaagcc ttatatcaag
    24241 aacacggttt ttctcaagtg acttatcacc atgggaaaat gtctttgatg acacctcgtg
    24301 gaatgattaa ggatgaaggt cttatgaaca ccctgaacat tatgagacga ggccttaaga
    24361 aagagaacag acaacagttt ttaaagatgc gtcgtttttt taccagaaca ggcaaaaatc
    24421 tctgctatat tgctgtaaaa agtgtaaaat aaaccttaga ccaacctgtc catacaacta
    24481 aacagaccat agaggagttc gaagatgtct tatatcaata atattgagca cgcgaaggtt
    24541 cttgatttga cgcaagaagt aatgattgag caagatcaaa tgttaagtag aacactagtc
    24601 caaaggcaag acctaggaat aacagttttt tccttagata agggccaaga aattgggcga
    24661 cactcatcac caggagatgc catggttact atcttaagtg gcttagcaga aatcaccatt
    24721 gatcaagaga cctaccgggt tgctgaaggt caaaccattg tgatgccagc tgggattcct
    24781 catgccttat atgcagtaga agcctttcaa atgcttttag tagtagtaaa accggaggct
    24841 taacatgatt gaaaatctct ctcaattggt gcaatatcat gatcatcagg tggctagccg
    24901 atccttgagc cgtctactgg gattatctca atctattgtt ttatatgcca tggcccaagg
    24961 agaaagcatt agtcaagaga ctagtccaag agataaagta atacttgttt tagaaggtca
    25021 gctcattttc gatttagagg atcaaaaaca ggtcctaact caagaaagtc tcatagccat
    25081 acctgcccaa aaagtccatc atttagaagc taaaaccgat tgtaaactat tacaagtgct
    25141 tttagattaa gggcttattg tcaactgtat tgggtggcct atggaaaagc aaaagagaag
    25201 gtgacgctca tcttctcttt ttacgtattc aaagcgatga caatacgaat tttaaagctt
    25261 ggctttctca tctccagatt aagcccttta gcttggttta ttttagaatt cactaaaaaa
    25321 accttaatga gtttcctatc tccttatagg tttctcatta aggcttttta gacgaaataa
    25381 ttgaacgaca ggcaaaagaa agatcttttt acgagtgata ccgttctcct atagttccat
    25441 aaatcctctt tttgatgttg ctgttagtta ttcttagcta aaagttatat tgttatcaag
    25501 acttgtctgt agtaacatgt tttaggagtt ccaggatacc gttattctct tagcaagctt
    25561 aactagctag agtggagtca ctatttataa taaactgtcg agcttctcga caaattggtg
    25621 atcgtctttg ttaatggcta gggtggtaaa accggtttct gtgggtaata taaagataat
    25681 atgttgctca gaattcttct tatctgtttt aaagacgtca aagagggatg tcgcagagac
    25741 atcagctctt tcaagtgttg ttggtaactg gtaacgttca aaaacttgaa ggaggctatc
    25801 caagtcctct tttggcaaga gtcctttgga aacagccagt ttagcatcaa ccaccatacc
    25861 gatagcaatg gcttcaccgt gatggtaaaa atcatggtgg cagagcgatt caatcgcgtg
    25921 gcccaaggta tgcccaaaat ttaaagacat gcgcaaacca ttttcaaatt catcttgttc
    25981 aacaatccgt tttttattgg aaatggattg gtaaatcaga cgttcaagtc cctgcttatc
    26041 ggtttctttt tgaaaatcac gtaactgctg aaaaagcggt ctatcatgaa tgaagccgat
    26101 tttgagcatc tcactgatgc cgcaggagaa ttcccgttgc ggtaaggtct ccaaaaaggt
    26161 ggttgaaata atgatagctt ctggagggta aatgctgcca atcatattgg ttagaccttt
    26221 aaaatggact ccaactttgc ccccgatact gctatccacc tgactaagta aagtcgttgg
    26281 gatttgaatg tagggaatac cacggtaata ggttgcagcg acaaaaccac ccagatcccc
    26341 aacaacacca ccaccaatag taacaatcat atcttttttg gaaaaatttt cagcgatgag
    26401 ctggtcgtag atagcactga cacgttccaa ggatttggat tgacctcccg gagggcagac
    26461 atggacaaaa gcgttgtatt gctgagcaaa ttcttcaaag agagtctgat acaagtggta
    26521 gaccgtttga tcggtgataa acaagagttt acgctgcttg cgctcaccaa ggcaatctgc
    26581 taaggttttt aaaacatggt cggtaaacag gatatcataa tccttgaccc gagaatggac
    26641 atgaagtgtt tgtggcataa gcactccttt catcagtact aggtaacaga gataacgcag
    26701 tgatatttag agcttaatct tgccaaagtt cctgccccaa ctgttctaat tgcttataat
    26761 caatttgctg ggcagcatct gacagcgcat ggtcgggatc aggatgaacc tccatcatga
    26821 tgccattggc accgacagcc ttcgctatct tagcagcagg taagagtaaa tctcgtctgc
    26881 ctgtcgaatg agatacgtcc acaataatcg gtaaatctgt cttttgttga ataataggga
    26941 ctgccataat atcaagcata tttctagtct ccacatcgta gccacgaacg ccacgttcgc
    27001 ataagataat gttagatttt ccagtgtctt gtaagtagga aagggcgccc aaatattcct
    27061 cgatagtagc catcaagcct cgtttaaaca aaataggctt atcgatgtgt gacaaggttt
    27121 ttaaaaattc aaaattttgc atgttgcggg caccaacctg aatgacatcg agatagtcat
    27181 aggcttcttc taattggcgc tcggacatga tttcactgac cgatagtaag ccaaattcct
    27241 gacaaacctc atgcaagtaa cgaattcctt gcaggccaag gccttgaaaa gaagcagcag
    27301 atgttcttgg tttataggcg cctcctctaa aatagttgta accaagtttt ttggcacttg
    27361 aagctgctaa tctaatgtga tcataagatt caatggaaca aggtccaaca ataaaattat
    27421 tcttagaaca agtcttattt tgaaagtcag acataatgtc aatcattcgt ttgattcctt
    27481 tactaattct gtatggcgtg ctctatggga taacgtctta tttaggaata caaaatctcg
    27541 ttgttatcca tgaagcaccc ttagcctgtg aaatgatacc ataaacaaga tgaaatagca
    27601 agtaaaccct agattgtcta tgttcttagt catttttatt aaaaaaggct tactagctat
    27661 aaaaaaatac tgccctaaat cttattagga cagtattttt ttatagatgc caccaccgag
    27721 aatcgaactc ggaacgaagc attaccattg ctttattata ccatttaact acagcggcta
    27781 cctttttata atactataag ttattaattg atgcaagctt ttcttaaaaa tatttcctta
    27841 attgtttatc atcaaaatga gggtaccagt tattaataac atggcacaag aggcctgcct
    27901 tatggtataa tggtaaagat aatttcagaa agaggaagcc aagcatgtct tatgaaaaag
    27961 aatttttaaa agattttgaa gactgggtta aaactcagat tcaagtcaat cagctagcca
    28021 tggcaaccag tcaagaagtt gcccaagaag atggcgatga aagggctaaa gatgccttta
    28081 ttcgttatga aagtaagtta gacgcctacg aatttttact aggtaaattt gacaattata
    28141 aaaatggtaa agcctttcac gatattccag atgagttatt tggagcaagg cattattaag
    28201 aaggagacac gaaatagtgg caaaacaaaa aaatcgtaaa aaagcattga aattagaaat
    28261 gaagcgtaat ggcttgttaa aaaaagcagg tcaagttttt gataaggcag ttgaaagtgt
    28321 tgaaacagct gtggataaaa ccatttctgc tggtaaaaat cttgtggaaa aaggcagcca
    28381 aacagttgaa aacctgacag caagcaagga aagacttact cttgatgagt ttgcagggca
    28441 gccagttgta gctggtatcc gctctgacct tgtcgaaacc ctatatgcag aaggcatcca
    28501 ttcagcgcaa gcttttaagg agtggacaga aaaagacctc cttgctctta agggcattgg
    28561 tccagcaacg gttaaaaaat tggtcgaaaa tggtgcaagc tttaaaaaat agtcatcttc
    28621 tcgttgctta atggctataa atttgttaga atattagcaa tcagaggtgc attgagttgg
    28681 tctgttacag agagcggtgg tgttgagaaa tccgcacaga tgtcaaggct agtagctgag
    28741 gatcagaaat cagatatgaa aagtagcaga cccattttat ggtctgaaac agtggtggta
    28801 ccgcggaaat cgtaacacga aattcgtccc tgtctaggtt atctaggcag ggattatttg
    28861 gtttcatttg aaagaagatg atggcttatt gttaaaatgt tgttagaagt atataataga
    28921 aaaactttgc ccaaagtgga aacagaacgg ctgctattac gtcaacgaaa aggggacaag
    28981 gcattgttcc agaagcggct tcagctctga ttgaaattgg ttttacctta cttgatttgc
    29041 ataaaattga gctgggttgc tacgactaca acaagcaaag tcaggcagtg gctcgcaaat
    29101 taggctttac cttagaagcc aatgcccgtg accgtaagga cgttcaaggt cgccgctgtg
    29161 gcgacatgcg ttttgggtta ctgagaagtg agtgggagga gcagaaacaa aaatgaacct
    29221 tatcatcatt ggtgctcaag cgtcgggcaa gatgaccatt gggcaagaag ttgccaggca
    29281 gacaggcatg actttgtttc ataatcacga ttctattgac tttgttttac ggtttatgcc
    29341 ttggtctcaa gagagcactg ccttgattga acgtatccgt tttgcttttt ttgaaacctt
    29401 tgctaagaca ggtcaagaca tgatttttac tattgtaatt gacttcaatg atccaaatga
    29461 tgtagcaatg cttgaaaaga ttcaggctgt ttttcagtct tatgaccaag aggttctctt
    29521 tgtcgaatta aaaacagata tagaggaacg tcttaaacgc aatcgcacag aaaatcgtct
    29581 caaacataaa cctcttaaac gcaacatcga gtggttagaa caagatattc aatccacgat
    29641 ggcatatgct gtttttaatc cagaagaacc acccaaaacg ctcacgcatt accaaaaaat
    29701 caataatacc cagctcaccg ctgctgaaac agcgcagctt attatccaaa aaatgactca
    29761 tatcaaggag aactaagatg acagaactat cacctaaata caatcctgcc gaggttgagg
    29821 caggtcgcta tcaaaaatgg cttgatgcag atgttttcaa gccatcagga gaccaaaaag
    29881 caaaaccgta ttctatcgtg attccaccgc caaatgtgac tggtaagttg cacttgggac
    29941 atgcttggga cacaacactt caagatatta tcattcgtca aaaacgcatg cagggctttg
    30001 atacgctttg gcttccaggg atggaccatg caggaattgc gacacaggct aaggtagagg
    30061 agcgtctgcg cgagcagggg atttctcgtt acgaccttgg tcgtgacaaa ttcctagaca
    30121 aggtctggga gtggaaagac gaatatgcca ctacgattaa agagcagtgg ggcaaaatgg
    30181 ggctttctgt ggactacagc cgtgagcgct tcacccttga tgagggcttg tcaaaagcag
    30241 ttcgcaaggt ttttgttgac ctctacaaaa aaggctggat ttaccgtggt gaattcatca
    30301 tcaactggga tcctgctgcc agaactgccc tttctgacat cgaggttatc cacaaggatg
    30361 ttgagggagc tttctatcac atgaactata tgttggaaga tggctcacgc gcccttcaag
    30421 tggcaacaac gcgaccagaa accatgtttg gagacgttgc cgttgccgta aacccagaag
    30481 atcctcgtta caaagatttg attggcaaaa atgttatttt accaatcgtg aataaattga
    30541 ttccaatcgt gggtgacgag catgctgacc ctgaatttgg gactggagtt gtgaaaatca
    30601 cgcctgccca tgatcctaac gatttcgagg ttggtcaacg ccataatcta ccacaagtga
    30661 atgtcatgaa cgatgacggt accatgaatg agttagctgg cgactttgca ggcatggacc
    30721 gttttgaagc tcgtcaagct accgttgcta aacttgaaga acttggcgcc ctagtaaaca
    30781 tcgaaaaacg tgttcactca gtaggtcact cagaacgttc aggtgcagtc gttgagcctc
    30841 gtctatccac tcaatggttt gtaaaaatgg atgagcttgc taaacaagct atggacaacc
    30901 aagaaacaga tgaccgagta gatttctacc caccacgttt caacgatacc ttcctccaat
    30961 ggatggaaaa tgtccatgac tgggtaatct ctcgccaatt atggtggggt caccaaattc
    31021 ctgcttggta caatgcagaa ggtgagattt acgttggcga agaagcacca gaaggtgatg
    31081 actggactca agatgaagat gttcttgaca cttggttctc atctgcttta tggccgttct
    31141 caactatggg gtggcctgac acagatgtag aagatttcaa acgttatttc ccaacctcaa
    31201 ccttagtaac aggttatgac atcattttct tctgggtgtc acgtatgatt ttccaatcgc
    31261 ttgaatttac cgggcgtcag ccattccaaa atgtcctgat tcacggtctt attcgtgacg
    31321 aagaaggacg caaaatgtct aaatcattgg ggaacgggat tgatccgatg gatgtcattg
    31381 agaaatatgg tgccgatagc ttacgttggt tcctctcaaa cggctctgcc ccaggacaag
    31441 acgtgcgttt ctcatacgaa aaaatggacg cctcttggaa tttcattaat aaaatttgga
    31501 atatttctcg ctatatcctc atgaataatg aagggttgac attggaagat gctgaaagta
    31561 atgtggctaa ggtagctgcc tcagaagctg gaaatgtgac tgaccagtgg attctccaca
    31621 atctcaatga aaccatcgct aaagtgacag aaaactttga caagtttgag tttggtgtgg
    31681 caggacacat tctttacaac tttatttggg aagaatttgc caactggtat gtggaattga
    31741 ccaaggaagt gctgtatagc gacaacgaag ctgaaaaagt tatcacgcgc tctgtccttc
    31801 tttatacttt ggacaagatt ttacgtctcc ttcacccaat catgccattt gtaacagaag
    31861 aaatttacgc tcaatatgcc caaggttcta ttgtgacggt ggactatcct gtggttcgcc
    31921 cagcttttga aaatgaagct gcgcataaag gtgtggaaag tttgaaagac ctgattcgtg
    31981 cagttcgtaa tgcgcgcgct gaagttaacg tggcaccatc aaaaccaatc accattttgg
    32041 tgaagacagc agatagtgaa ttagaagact ttttcaacag caatatcaac tacatcaaat
    32101 gcttcactaa ccctgaaaaa ttagaaatca gctcagccat tgctgcgcca gaactggcta
    32161 tgaccagcat catcacaggt gctgaaatct acttgccact agcagatttg cttaacgtgg
    32221 aagaagaact tgctcgccta gataaagaac tggctaaatg gcaaaaagaa ctggacatgg
    32281 tcggcaaaaa actcggcaac gaacgctttg ttgccaacgc caaaccagaa gttgtccaaa
    32341 aagaaaaaga caaacaagct gactaccaag ctaagtacga cgcaacccaa gaacggattg
    32401 cagagatgaa gaaaattaaa tcatagtgtt tagaacgcag ttgaaaaact gtgttctttt
    32461 cttactaata gagatattag attgaagttt aattatgaga aaaacgcttt ctttatttaa
    32521 agagttatgt tacaatacta ttacaatcat cgtacaaata gtgttatttt gagaggcata
    32581 tgagaatgat tttagcacac tatgactgta aaaaagataa aaagcaatct ttagatgagc
    32641 atttatggca tgtggcctgt tctagtcgac aggaagcatc tataattggt caaggagatg
    32701 tgcttttttt aattggtctt taccacgacc tgggcaaagc tgatcgaacc tttcaagata
    32761 aattattaaa taatccaaat cggcatgttg atcactctta tgcaggggca aaatacttat
    32821 gttctattat tgggcctcat ctaaaaaacc gaggggttga taaaaatgag agaatgacat
    32881 tcaacgaaat ggtggggtat gtcatctctg ctcatcatgg gatgtatgat ttatgctact
    32941 attttgacga tgctgaatat tatggcttta ataagtttaa aaatcgtatc aatagagact
    33001 tagatggtta tcactatcat gaagatatta aagggtacgc tctaaaatta gaaaaaaaat
    33061 tatgtgatta tggctacaaa gatttaaggg agcttattga taaagctttt gataattacc
    33121 aacaagccat gtcttcctta aactggcaag ataagagtga gtgggattat tatcagtctt
    33181 gtatggtgag actttacttg tcactcttaa aaaacgctga tattttggac acagtaaatg
    33241 cctatggcct taagataagt cctatggata aaacagagcg atcctttcta aaacactcct
    33301 atttagcggc cattgaacaa aaatatgcta gctttggaca gccaaacaat cagttgaaca
    33361 ctattcggac agaaatcgct gagcgtgtta aagaaagagg taaacgagat tccaagggga
    33421 tttatcgctt agatttaccg acaggagctg gcaagactaa tcttagtatg cgttatgcgt
    33481 ttcaccaatt agttcatcac gacaaatcaa ggttttttta cataacgccc tttctttcgg
    33541 ttcttgagca aaatgcttcc gaaattagaa aagttacagg tgaccttggc gttctagaac
    33601 accattccaa tgtggtgaaa caggctaatg aagatgatga tgataaggac agtttattgt
    33661 cagcttatct tagtgatagc tgggacagtc aagtagtctt gacttctatg gttcaatttt
    33721 tccaaacact tttcaaaaca aaatcagcta atctgagacg tttttcaagt ttgattaata
    33781 gtgttgtgat tctagatgaa gttcaatccc tgcctattga agtcaccact ttgtttaatt
    33841 taacgatgaa ttttttaaat aaagttatgg atacaaccat cgttctttgc acagcgacac
    33901 aacctgctta tgattcttca gagattgacc atcgtatctg ttatggaggg aacttgggag
    33961 aattagctga aatagttgag ttaacgattg aagaaaaaca gattttttca aggacagagc
    34021 ttagaaaatt tgatgatagt gatcagaaag ttcacttgac tgatgttatt aaccttattc
    34081 taggtgagga aaactcagtt cttgctattt ttaatacgaa aaaaacggtt cataactgct
    34141 atactatgct aaaagacatg actgatagac cggtctatca gctttcgaca aatatgtgtg
    34201 cgcagcatag acttgacttg attgctaaga tcaaaacgga gttacaaaat aatatcccta
    34261 ttatttgtat tagcacgcaa ttaattgaag caggtgtaga tgttgatttt catcgcgtca
    34321 ttcgttccta ctcagggatt gattctattg ttcaggctgc tggacggtgt aaccgagaag
    34381 gcaaacgaga taaagggcaa gtcactcttg tcaatctgac caatgaagag gaaaatattt
    34441 ctaggctgac agaaataaaa actaaaaaag aagccacaga atctattctt cataagattg
    34501 ggtctccaat tgatatctca actttaaacc gtgacttttt tgagtattat tatgccaata
    34561 atcagggact gatggattat cctttggaag acaacctatc aatctacgac tatttaagcc
    34621 ttaatattta tcagacggca aataaaaagt tcaaaggtaa gttaaaacaa gcttttaaaa
    34681 cagcaggagc caaaatgaac ctcatcaata atgatatgat aggaattctc gtaccttatg
    34741 gcgaagctga gaaaaaattg gcttatttag aagaattagg tgtgtcacat tttttatcag
    34801 caaaagatta tcaaacgata aaatcattac taaaagagtt acaacctttt acggttaatg
    34861 tccgcgagaa cgatcctctc tttgagacaa caaaatctta tctaaatggt cagattctgg
    34921 ttttgacgtc ggagtattat gacacggaaa gaggagttaa atacgattca gctagctttt
    34981 acttctaact caaaacgaaa gaagattaac aaaaggttgt tagaggacct tgttaacctg
    35041 ccaatcatca ttagtaatta ttatcaattt agactattta ataaaattag attacaaaaa
    35101 aacagaagga ggaaagtagc ttgtacagat ctagagactt ctacgtgaga gtaagtggtc
    35161 agcgagctct ttttacaaat ccagccacaa aagggggatc ggaacgctca tcctattcgg
    35221 ttccgactag acaggcactg aatggtatcg ttgatgccat ctattataag ccgaccttta
    35281 ctaatatcgt cacagaggtt aaggttatta accagattca aaccgaatta cagggtgtca
    35341 gggctctgtt acatgattat agtgcagatt taagttatgt atcctatttg agtgatgttg
    35401 tttatctgat caagtttcat tttgtttgga atgaagatag aaaagatttg aactcagata
    35461 gacttccagc taaacatgaa gccattatgg agcgttctat tcgtaaaggg ggacgtcgag
    35521 atgtgttttt gggtacaaga gaatgtttag ggcttgtaga tgatatcagc caagaagagt
    35581 atgagactac tgtgtcgtat tataatggtg tcaatatcga cttgggaatc atgttccatt
    35641 cctttgccta tccgaaggac aaaaagacac cattaaaatc atactttaca aagactgtga
    35701 tgaaaaatgg agtcattacg tttaaagcac agtctgaatg cgatattgtt aacacgcttt
    35761 ctagttatgc ttttaaagca ccagaggaga taaaatcggt taacgatgaa tgcatggagt
    35821 atgatgccat ggagaaagga gaaaactgat ggattttttt acttctctct tgaagactta
    35881 tgaaaaagca gagctagcag acttggttga tcatcaaaaa agaaataatg agccggtttt
    35941 actgccgatt tatcatacga gtttaaagtc aaatggtaaa aatatcattt cagtgaaact
    36001 tgacaaagat ggccagtttc acaaggcaga atttatggca gataagcaaa tgattatttt
    36061 tcctgtaacg gctgattctg ttgctaggtc aggtagtcat cctgcaccgc atcccctagt
    36121 cgataaattt gcttattata gtgctgaaat ggggcagatt cagtatgatt cttttcataa
    36181 gcaactgaat aactggattg attattgtga ggagggtgat gtcaagaaat ttttaacctt
    36241 tgttcagcag ttcattttga agccagaatt tctaacattg attcttgatt ctttaattgg
    36301 tcctgattat caacataatc aattaaaagt cacattttgt gatgccactg gaaaagaaaa
    36361 attaattgat ttatcagctt gctttttaga attttcaatt gatcagttcc agggctttaa
    36421 aaatgaatcg gtttcgacat ttaaagcctt acaccaatcc tatatttctt ttgttgaagc
    36481 caatcgtgaa aatctcggta tttgtaatat tagtggacga gaggaacagc ttaccgataa
    36541 gcatagaggt ttgatgggga atgctaaaat catctctgtt agtaataaaa gagaagctta
    36601 taaaggacgt tttagagaac gcgaagacgt ttttagtgtt ggctatgaaa cttccgaaaa
    36661 gattcattta atgctcaagt accttttaga aaataaaaat accagtactt ggttagggtc
    36721 ttctcaatat ttaatcaact ggttcagcga tgatttaaca aatgatagtc ggttggatat
    36781 tgtatcacca atctttgatg atggacttga agaagaagat gatgacgata cgcctcctgt
    36841 tataacatta gcaactgaag acaataaaag aattggtaaa tcattcatca agggacaaaa
    36901 attatttgct aatgatgcca cttactacgt tgctattttg aataaaacca gcaatgggcg
    36961 gattgcttta aaatattttc gtcagcttca agcgtcccaa ttactcacca atcttaacaa
    37021 gtggcaggaa acatacagtt gggagtcgcg atctaagttt gggaaaagtc gcttaagaac
    37081 ccctactttt catgacatcc ttaatgtgtc ctacggggtt gatagggatc gcttccttga
    37141 attagataat gataacttca aaagtgatca aattcaaaag ttagtggcaa gtttgattga
    37201 tggtaaaccg atgccacagt ccattgtcaa aaagttaggt aacaatgtta aagaacgaca
    37261 tcgttaccgt aagcactggt atcaagttga gcaggtctgc ttagcaattt tacacaaaca
    37321 aaatggggag gaattttcac cgatgctaga tcataccaat caaaatcgtt cctatctttt
    37381 tggacgatta ttagcaattt ttgaattaat cgagaccttg cgttatggct tggatggaaa
    37441 caataacgac cgtattacca atgctgaacg ttattggaca gcctatactg gacaaccaac
    37501 aaaattgatg atgttattgg aaaataaaat taagccttac gaagaaccat tgaaattaaa
    37561 tcgtcgtggc agttggatga aattagaaaa agaaaaagaa gagattttag aactgttaaa
    37621 tcctctgtta gaaacagaaa caatggaaaa acccttagat taccgcttta tttttgggta
    37681 ttatgctgag aaaaactatt actatacaaa acaaaacacg gaagtaacag aaagtgagga
    37741 gtaaaaagat gttggaacac aaaattgatt ttatggtaac tcttgaagtg aaagaagcaa
    37801 atgcaaatgg tgatccctta aatggaaaca tgcctcgtac agatgccaaa ggatatggtg
    37861 tgatgagtga tgtctccatt aaacgtaaga ttcgtaatcg tttgcaagat atggggaagt
    37921 ctatttttgt gcaagctaat gagcgtattg aagatgattt tcgttcactg gaaaaacgct
    37981 tttcgcaaca ttttacagct aagacacctg acaaagaaat tgaagaaaaa gcaaatgcat
    38041 tatggtttga tgttcgtgct tttggacaag tttttactta tctgaaaaaa tcaattgggg
    38101 tgcgtggacc agtttccatc agtatggcta agtccttgga gccaattgtc atttccagcc
    38161 ttcaaattac gcgtagtacc aatggtatgg aagctaagaa taatagtggc cgctcttctg
    38221 atacgatggg gacaaaacat tttgtagatt atggtgtgta tgtacttaaa ggttctatca
    38281 atgcttattt tgctgaaaag actggttttt ctcaggaaga tgctgaggct attaaagaag
    38341 ttttggttag cttgtttgaa aatgatgcgt cgtctgcacg tccggaaggc tctatgcgag
    38401 tttgtgaagt cttttggttt acgcattcaa gcaaattggg aaatgtttca agtgcgcgtg
    38461 tctttgactt gttagagtat catcaatcaa tagaagaaaa aagcacttat gacgcttatc
    38521 agattcatct aaatcaagaa aaattggcta aatatgaagc gaaagggtta acgcttgaaa
    38581 tcctagaagg actctagtat ggtctatgcc gaagatgatt atttaatgct gtcaggtatt
    38641 cagcatttcc aattttgtaa acgtcaatgg gcgttgatcc atattgagca acaatggctt
    38701 gataatgaag cgacagcgca tggacaggtt ttacatacta aagcagataa cccttacatt
    38761 aaagaaaaac gaaaagagct tttggtctca cgtgctatgc ccatttcttc tgcagaactt
    38821 ggactttcag gaattatgga tgttgtggaa ttttataaag atgatcaagg tgtgtctttg
    38881 aggggaaaac gtgggaaatg gttaccaaaa gttgtggaat acaagcgcgg aaaacctaaa
    38941 aaagatacca gagatattgt ccagttggtg gctcagacca tgtgtttaga agaaacgcta
    39001 gactgcgaca ttaacgaagg ttgtctttat taccatagtg tcaatcaaag agtgattgtt
    39061 cctatgacat cagctttgcg tcaagaagtg aaggaattag ccgcagagat gcatgaggtt
    39121 tatcagagtc aaatgctacc taaagcagct tattttaaaa actgtcagct ttgttcttta
    39181 gtcgatattt gtaagcccag gttgagtaaa aaaacaagga gtgtgtcgcg ttacatcaat
    39241 gaggctatga ccagtgagga gatggaccta tgaagaagtt gctaaatacc ttgtatttga
    39301 cgcaagaaga tttttatgtc actaaagagg gcgataacat tgttatcaag caagaaggta
    39361 aggttctcaa acggtttccg tttcggatta ttgacggtat tgtctgtttt tcttatttgg
    39421 gtgtgtcgtc tgctttggtg aagttatgta cggagaatca gattaattta tcgtttcata
    39481 caccacaagg gcgtttttgt ggtcgctata ttggttcaac caatgggaat gtgttgttgc
    39541 gtagagaaca ttatcgttta tctgatcgtg aggaatcttt ggaatacgca aagcggttta
    39601 ttttggctaa aatttccaac tcaaggaaat acttgctacg ctttaaacga gatcatcgtc
    39661 aacagattga taccaagctt tttgaggctg ttaatgacga attgatatgg gctttagaga
    39721 tggttcaggc agcagataat aaagactctt taagagggat tgaaggccaa gctgctaatc
    39781 agtattttcg catatttaat gacctggtgt tgacggacaa aaaaacgttt tacttccaag
    39841 gtcggagtaa acgaccaccc ttagattgtg ttaatgccct cttgtctttt ggttacagtt
    39901 tactgacctt tgaatgtcaa tctgccttgg aagctgtcgg attagacagt tacgttggtt
    39961 tctttcacac ggatcgtcct gggcgtgcta gtttagcgct tgatttagtt gaagagttcc
    40021 gctcatatat tgtagatcgt tttgtctttt cattaattaa taaaggacaa cttcagaaaa
    40081 aacactttga ggttaaagaa aatggtagta ttttattgac ggaaaatggc agagctattt
    40141 ttattgattt gtggcagaag cgtaagcata ctgaggtaga acatcctttt acaaaagaga
    40201 aagtaaaact tatgttatta ccctatgtac aagcgcagct tttagctaag gctatacgag
    40261 gagatttaga aagctatcca ccttttatgg tttaggagat gttatatgat ggttttagtc
    40321 acttatgatg taaatacgga aacacctgct ggtagaaaaa gattgcgtca tgttgccaaa
    40381 ctctgtgtgg actatgggca acgtgttcaa aattctgttt ttgaatgttc tgtgacaccc
    40441 gcagaatttg tggatataaa gcaccgctta acacaaatca ttgatgagaa aactgatagt
    40501 attcgctttt atttattggg gaaaaattgg cagaggcgtg tggaaacact tggtcgctca
    40561 gacagctatg acccagataa aggtgtctta ttattgtaaa aatctcttgt gcgaagctag
    40621 ctttcacaga aacaccttgc ttgctcgcgc aaaaataact taaaaaagaa gcgaaatgga
    40681 gataaaaagg cttaaaacac ctctatgtgt gccatccgtt tctctataaa ctgtgtcatt
    40741 tggcgcagtc tcacccttca tgggtgagtg gatcgaaata aaaagcatat cacgaaaatc
    40801 accaattaca tcaggtctca cccttcatgg gtgagtggat tgaaataagt cattttctgc
    40861 ttctgctagg tttgctttag tctcaccctt catgggtgag tggattgaaa ttttttactt
    40921 tgattacatc ccgcaatgtc acagcgtctc acccttcatg ggtgagtgga ttgaaattgt
    40981 ccgcatacct tgatttgagc gagtaaactc gtctcaccct tcatgggtga gtggattgaa
    41041 agatgaaccc gatctctctt ttaatgaggt cgggttttta catgccttta gtcaaccatt
    41101 tatctgatag cgtatcaaat tcaagttttt gcatgttcta gtctatggtt tgcgccacta
    41161 gctttcggta aactaagggt aacttaagag aggagttatt tatgacacta tcacttgtat
    41221 taatggtatc ggtttttggg gcaggcttgt tatccttttt ctctccgtgt atctttccgg
    41281 tccttcctgt ctatttaggc attttactgg atgcagatga ttcaaagacg attactatat
    41341 ttggtaaaaa actctattgg tatggcattg tcaaaacttt agcctttatt tttggtctat
    41401 ctactatttt tgtgatttta ggttacgggg ctggtttttt aggaaatatc ctttatgcgg
    41461 tatggtttcg gtacttactg ggagccttgg tcattatctt ggggattcat cagatgggcc
    41521 tcattaccat taagagctta caatttcaaa aatcactgac tttccataat aacaagaatc
    41581 gcaacggttt gttcaatgca tttatccttg gtttaacctt tagctttggt tggacgcctt
    41641 gtgtgggacc tgttttgagt tctgtcctag cattggtggc ttcaggggga aatggtgcct
    41701 ggcaaggtgg cgttttgatg attatttata ctcttggatt gggcattcct ttcctactca
    41761 tctcttttgc gtcaggcatt gttttgaaac agtttaacaa gctcaaaccc cacatacttt
    41821 tactgaaaaa agtaggaggc gttctgatta tagtcatggg aatcttgctt atgacaggaa
    41881 ccttaaataa cttagcacaa ctttttggat aaaggagaaa cacaatgaaa aaaggactat
    41941 tagtaacaac tggtttggct tgtctcgggc tactaactgc ttgctcaacc caagacaata
    42001 tggctaaaaa ggaaataact caggacaaga tgagcatggc agctaaaaag aaagataaga
    42061 tgtcaacatc aaaggacaag tccatgatgg cagataaatc atctgataag aaaatgacca
    42121 atgatggtcc tatggtgcct gatttcgaac tcaaaggcat cgatggtaag acctatcgct
    42181 tatcagagtt caaaggtaaa aaagtttatt tgaaattttg ggcctcttgg tgttcaatct
    42241 gtctatcaac cttggcagat accgaagacc tggctaaaat gtcagataaa gactatgtgg
    42301 tcctaacagt tgtctcgcca ggtcatcaag gggaaaaatc agaagcagac tttaaaaaat
    42361 ggttccaagg aacagactat aaggacttac cagtcttgtt agatccggat ggcaagctat
    42421 tggaagctta cggtgtcaga tcttacccaa cagaagtttt cattggaagt gatggtgttc
    42481 ttgctaaaaa acacattggt tatgccaaaa aatcagacat caaaaagacc cttaaaggta
    42541 tacattagga aggaccagct gataggcatt agaaaaaagg agttgagttt atggaacata
    42601 aatggaaatt atggctattt atcgttggag tggtcttgtt aataggcgga gcttctgttc
    42661 tcattcagaa aggtcaagcc aaggttttta gtcaaggcaa gcctcaacta ccagctaaaa
    42721 aagtcaaatc cactaaagta gatcccaatg cccctgttga tcaaaaagtg atttacctgg
    42781 ctggaggctg tttctggggc gttgaagaat atttttcaca agtggatggc gttctagatg
    42841 cggtgtcagg ctatgccaat ggtagaggcg ataccaccaa ctaccagtta attcatcaga
    42901 ctggtcatgc agaaaccgtt gaagtggcct atgatgcgaa ccgtattagc ttgaaagaat
    42961 tattactgca ttttttccga attattgacc caaccagtct taataagcaa ggaaatgatc
    43021 gcggtagtca ataccgaact gggatttact atactgacaa agcagattta gctattattg
    43081 atgaggtctt caaagaaaaa gctaaggatt acaagaaaaa aatagtggtt gaaaaagcac
    43141 cattgaaaca ctttattaag gcagaagact accaccaaga ttacctcaag aaaaatccaa
    43201 atggctactg ccatattgac atcaaccaag cgacttatcc tgtgattgat gaaagcaagt
    43261 acccaaaacc aagtgccact gagatcaagg aaaaactatc tgcagacgaa taccgagtga
    43321 cgcaaaaaaa cgaaactgaa aaagcttttt ctaatcgcta ctgggattct tttgatgcag
    43381 gaatttacgt agacgttgtg acaggagaac cgcttttctc ttcaaaagac aaatttgaat
    43441 caggttgtgg ttggccaagc tttagccgcc ccatcagccc agacgttgtc cgctacaagg
    43501 aagataagag tttcaatatg acgagaacag aagtgcgcag ccgttcaggg aattctcatc
    43561 tggggcatgt ctttactgac ggacctaaag accaaggcgg ccttcgttac tgcatcaata
    43621 gcttgtctat tacctttatt ccaaaatctg atatggaagc taagggctat ggttatttat
    43681 tatcatctgt agaatagggt taggcctaaa aatctgatat aatagaagaa agactagtat
    43741 gagagggcca tgcaaggccc tttataaaga taaggagaca ccgtgtgtac tcattgttaa
    43801 ttgtagaaga cgaatacctt gtgcgccagg gtattcgttc tttggttgat tttagccagt
    43861 tcaagattga tcgggtcaac gaagcagaaa atggccagtt ggcttgggac ttgtttcaga
    43921 aagagcctta tgatattgtt ttgacggata tcaatatgcc caaattaaat gggattcaac
    43981 tagcagaact cattaaacag gaatcccccc aaactcatct ggtatttttg acgggctacg
    44041 atgattttaa ctatgcctta tctgctttga aattaggggc agatgattac ttgctcaaac
    44101 ccttttccaa ggcagatgta gaagacatgt taggaaagct ccggaaaaaa ttagaacttt
    44161 ccaagaaaac agaaaccatt caggaattgg ttgagcagcc tcaaaaagaa gtatcagcaa
    44221 tagcaatggc tattcatgag cggttggcag attctgattt gaccctaaaa agcttggctc
    44281 agcagcttgg ttttagccct aattacctta gcgtcttgat taaaaaagag ttagggatgc
    44341 cctttcaaga ttatctggta caagaacggt tgaaaaaagc caagcttctc ttgttaacca
    44401 gtaatcttaa aatctatgaa attgcagagc aggtcggttt tgaggacatg aattattttt
    44461 ctcagcgctt taagcaactg gtaggcgtta ccccaagtca gtataaaaaa ggaggccagg
    44521 catgaagcgt tatcccctac tggtccagtt gatttcgtat gtttttgtga tcgtaattgc
    44581 cctcattacc acgcttggtt tgctttatta ccagacgagt tctcgtaata tcaggcaact
    44641 aattgaacgt gatacccggc agagcattcg gcaaagttcc caatttattg acgcttacat
    44701 caagcctctt aaagaaacaa cttcggtgct ggcgaaaaat acggaaattc aagcctttgc
    44761 tagtcaaatt catcaagaaa atgacaaaca ggttcttcag ctcatgaaga tggttcttgc
    44821 gaccaattcc gatttacaag cagctgttct ggtgactaag gatggtcgaa cggtgtctac
    44881 caattctcag ttgaccatga aaacctccag tgacatgatg gcagaaccct ggtataaagc
    44941 agccatagac cgtcaagcca tgccaatctt aaccccagct cggcaattat ccctttcttc
    45001 taaaaaagaa tgggtagttt ctgtgaccca agaggtagta gatagggctg ggcataattt
    45061 aggtgtgcta agacttgata ttgcataccc gaccattaaa gcgtctttag atcagcttca
    45121 gctaggccgc caaggctttg cctttattgt gaatgataag catgaatttg tttaccatcc
    45181 caaaaagagt gtttacagtt cttctaagga gatggctgcg atgaaacctt atttagcgat
    45241 tcagaatggt tacactaagg acaagacatc ttttgtttac caaaaactca ttcctaacag
    45301 tcaatggact ttagtgggag tggcgtcact ggatcagttg caccgggtgc agcgccaaat
    45361 tttttggtcc tttcttggaa cagggcttct agcctgtctg atttgtggct ttgcaactgt
    45421 cttagtctta cgcagatgga ttcgtcccat tcaacaattg cagcaagtta ttcttgctat
    45481 tcaaaaagga gatcgtcagt tacgtgccca agaaacgggt tctccagaat tgacagacct
    45541 tgcccaacag tttaatgcgc tcttagatca aattgatagc ttgatggttg ccgttgcgga
    45601 taaggaaaag gcgattgggc agtataggtt acaagccttg gctagtcaga ttaacccgca
    45661 ttttctctat aacaccttgg acactattat ttggatggca gaatttaatg acagcaagcg
    45721 cgtggtagaa gtgaccaagt ctctagctaa gtattttcgt ttggccctta atcaggggaa
    45781 cgaatacatt cgtttggcag atgaactgga tcacgttagc caatacctct ttattcaaaa
    45841 acagcgctat ggagacaagc taagttatga agtgcaaggc ttagatgtct acgcagactt
    45901 tgttattcct aagcttatct tacagccctt agtagaaaat gctatctacc atggcatcaa
    45961 agaagtcgat cgcaagggca tgatcaaggt tacggtatct gatacagctc agcatctgat
    46021 gttgactgtt tgggataatg gtaaaggcat tgaagactct tcactgacca atagtcagag
    46081 cttgttggct aggggaggtg tgggccttaa aaatgttgac cagcggttaa aacttcacta
    46141 tggtgaaggc taccacatga ccattcatag ccagtcagac cagttcactg aaatacaatt
    46201 aagccttcct aaaatgcatg aattaatggc agacgacaca caggaaaacg agtaaggtta
    46261 caggaagtgc atgtcagcca tttccctaag ttagggtatt ggagatagga accttagaaa
    46321 ggatatcatg agtttagcaa ctgactattt tagccgacag acccccatag ttgaaaagct
    46381 aatggcttac ggttttgaga agcgagacaa cggttatttc tataacgaac ggtttatgga
    46441 aggggaattt gaggcgcagc ttaggattga tgaggctggc aatatctggg atcgggttat
    46501 tgattgtgac ttggaggagg attatttgcc tttacaacaa gcagcctggc aaggaaccta
    46561 tactgggcag gttagagcgg cctatttgga acttttggag cgcttatcag tagcctgttt
    46621 tgaagccact ccttttcagt caatgcaagc taatagactt gctaagcata tcaccaaaga
    46681 atggtctgac ccaatggact atccttttga aaagcaccca gaccttgcaa cctatcgggt
    46741 agggggcaaa tggtatgcta tgattttttc tctcttggct gataaattag accagatacc
    46801 agagagatta gtaggacaga catgtgaagt gatgactgtc aaagttaatc ccaaggcttt
    46861 ccctcagtta ctgcagcaag aaggcattta cccagcgtat cacatgtcaa agaaaaactg
    46921 gattagtatt attttggatg ataaggtaac agatgataag ctttggacgt tagtgactca
    46981 aagtcgtcaa ctagtcaacc caaacggtct ttctaatcct aatggtcctg attattgggt
    47041 tattccagct aatttgaaat actatgacat tgatgcagaa tttgcagcca atgatgtgat
    47101 tttatggacc caaaaagctg gtattgccgt gggagactac gtgctgattt atataacagc
    47161 cccagtcaaa tccattcgct atgtctgcca agttctagaa actgatatac ctaacgaggg
    47221 ttatcgtaaa aatcctaaca ttgacaaact catgcgtctc cgaaaatgcc aacagtacaa
    47281 ggatggtctc ttgtcttttg accttatgaa aaaacatggc gtagctgcag tgagagggcc
    47341 aagacgatta agccctcaat taattgcatt tttaaaggaa aaagagtatt ttaaagagaa
    47401 caactaaagg agaataagag atggttgaat ttgtagcaga tcaaagtttt tgggaacttt
    47461 ttccaggtgc caaactaggt gtcgtcttat taaaggatgt tcagaaccaa gcagagtcac
    47521 cagaagatgt caaacagctc ttagcagaca gccatgagtt agcaaaagct tatttaacag
    47581 cagacaattt tagtgataat caggtggttc aagtctaccg caaggcctat caacacttta
    47641 aaaccaaaaa aggagctcgc tctagcattg aagcccttct caagcgggtg tcaaatggtc
    47701 aaagcattcc gtctattaac ccactagtgg atatttataa tgcggctagt ctacgttttg
    47761 gtttacctgc tggagccgaa gatagtgaca gttttatcgg agatttgcgt ctgacgataa
    47821 cagatggtgg tgacgacttt tacttgattg gggatgctga caacaaccca accttgccaa
    47881 atgaactctg ttataaagat gatattggag cggtttgtcg ttgccttaac tggcgagatg
    47941 gcgaacgcac catggtgaca gaacacacca aaaatgcatt tctgattatc gaagctcttg
    48001 atcaagaagg tcaaaataga ttgcaagaag ctctgaaatt cattgaaggc agcgcaaaaa
    48061 tgtaccttca tgcaatcact tctgtgcatg tgttggataa agacaatcca cacgtgccac
    48121 tttttacaga aaactagatt agtaggatga gctgtgtaaa aatagaaggt taacaaaaag
    48181 cgagtaaacg aatgtcctgt catttaggtt ttcgtttact cgctttttga tgagatttta
    48241 ctgctgtcct agcaaaaggt gaggatgagg tcgtatatca catagaggtt ttctgttaag
    48301 ccttatttgt tactaaagaa gaatggtggc gtgtaatgac taagggtttc gtaacaggcc
    48361 ttagcttgct cattgtcttc acagacaatc aagcaagtat catcaccaca gaccgttgct
    48421 acaatttctg gaatttgcat ggcatctaaa atggagccaa aggattgggc tagtccaggt
    48481 aacgttttta aaataatttg atgttgaact atctttaaca tgaccaaagc gtcttccatg
    48541 taaaaacgaa gacgatgttc ccagcgggtt tgtgaaatgg ctaacgcttc atagtgtgtg
    48601 tcgtttccag aagttacttt gaccaggttc aattccttca tgtcacgtga tagggtagcc
    48661 tgagtgatcg tgatgccatt tttttgcaac cgttcttgta attcttgctg tgtatgtata
    48721 gttgtctcag agatgaggga acgtatgagt tggtgacgcg tctctttctt attcattgtt
    48781 ataatcttcc tttaacgtaa ttgataacaa tccgagtata gcaaaaagag gagaaggtgt
    48841 aaagtttttg atgaaccgct tacaaaatgg cttttttatg gtaaaataaa gatgatgttt
    48901 agaaaaagga gatttgtgtg attcgaagag aagattatca ataccttaga aaactaaacg
    48961 attttcgcta cttttctatt gaacaatttg acaaaattgt agggcagatg gaattccgaa
    49021 aagctaaaaa ggaccatatc cttttttttg agggagataa aagagataag ctttttctag
    49081 tgacctctgg ttatttcaag gtggagcagt ccgatcaatc aggaaccttc atgtatacgg
    49141 atttcatcag acacggcacc atttttcctt acggtggcct ctttacagat gattattacc
    49201 atttttcagt ggtagccatg acagacgtga cttattttta ctttccagtc gatttatttg
    49261 aagattattc gcttgaaaat cgtctacaaa tgaaacactt atatagtaag atgtctaagc
    49321 tgctcgagtt acatgagttg agggtccgaa acttgattac atcaagtgct agttctcgtg
    49381 tgattcaatc tctggcgatt ttgctggttg aaatgggtaa agacagtgat accttacctt
    49441 ttcaattaac aacaacagat attgctcaaa taagtgggac gacaagagaa acagttagcc
    49501 atgtgcttag agatttgaag aagcaagaat tgattaccat aaaaggtaag tatttgactt
    49561 atctagataa agactatttt ttacaataca caaagtgaat taaaggtcag aacactttat
    49621 tttaaaagtt tttc
//
LOCUS       DAXWIQ010000010        47659 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000010 DAXWIQ010000000
VERSION     DAXWIQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47659
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.010"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            167..1159
                     /gene="asnA"
                     /locus_tag="ACGEUI_000542"
     CDS             167..1159
                     /gene="asnA"
                     /locus_tag="ACGEUI_000542"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFF8165401.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLIAKLDVVEVQGPILSRVGDGM
                     QDNLSGTENPVSVNVLKIPNATFEVVHSLAKWKRHTLARFGFNEGEGLVVNMKALRPD
                     EDSLDQTHSVYVDQWDWEKVIPDGKRNLAYLKETVETIYKVIRLTELAVEARYDIEAV
                     LPKKITFIHTEELVAKYPDLTPKERENAITKEFGAVFLIGIGGVLPDGKPHDGRAPDY
                     DDWTTETENGYHGLNGDILVWNDQLGSAFELSSMGIRVDEEALKRQVEMTGDQDRLGF
                     DWHKSLLNGLFPLTIGGGIGQSRMVMFLLRKQHIGEVQTSVWPQEVRDSYDNIL"
     gene            1282..1821
                     /gene="rsmD"
                     /locus_tag="ACGEUI_000543"
     CDS             1282..1821
                     /gene="rsmD"
                     /locus_tag="ACGEUI_000543"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFF8165402.1"
                     /translation="MRVVSGEFGGRPLKTLDGKITRPTSDKVRGAIFNMIGPYFNGGR
                     VLDLFAGSGGLAIEAVSRGMSAAVLVEKNRKAQAIIQDNIIMTKAENRFTLLKMEAER
                     AIDCLTGRFDLVFLDPPYAKETIVATIEALAAKNLLSEQVMVVCETDKTVLLPKEIAT
                     LGIWKEKIYGISKVTVYVN"
     gene            1811..2302
                     /gene="coaD"
                     /locus_tag="ACGEUI_000544"
     CDS             1811..2302
                     /gene="coaD"
                     /locus_tag="ACGEUI_000544"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFF8165403.1"
                     /translation="MLTKIGLYTGSFDPVTNGHLDIVKRASGLFDQIYVGIFDNPTKK
                     SYFKLEVRKAMLTQALADFTNVIVVTSHERLAIDVAKELRVTHLIRGLRNATDFEYEE
                     NLEYFNHLLAPNIETVYLISRNKWQALSSSRVRELIHFQSSLEGLVPQSVIAQVEKMN
                     EKT"
     gene            2289..3326
                     /locus_tag="ACGEUI_000545"
     CDS             2289..3326
                     /locus_tag="ACGEUI_000545"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFF8165404.1"
                     /translation="MKRLKKIKWWLVGLLALISLLLALFFPLPYYIEMPGGAYDIRTV
                     LQVNGKEDKRKGAYQFVAVGISRASLAQLLYAWLTPFTEISTAEDTTGGYSDADFLRI
                     NQFYMETSQNAAIYQALSLAGKPVTLDYKGVYVLDVNNESTFKGTLHLADTVTGVNGK
                     QFTSSAELIDYVSHLKLGDEVTVQFTSDNKPKKGVGRIIKLKNGKNGIGIALTDHTSV
                     NSEDTVIFSTKGVGGPSAGLMFTLDIYDQITKEDLRKGRTIAGTGTIGKDGEVGDIGG
                     AGLKVVAAAEAGADIFFVPNNPVDKEIKKVNPNAISNYEEAKRAAKRLKTKMKIVPVT
                     TVQEALVYLRK"
     gene            3740..4716
                     /locus_tag="ACGEUI_000546"
                     /pseudo
     CDS             3740..4716
                     /locus_tag="ACGEUI_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
     gene            5164..5784
                     /locus_tag="ACGEUI_000547"
     CDS             5164..5784
                     /locus_tag="ACGEUI_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFF8165405.1"
                     /translation="MKKEISPEMYNYNKFPGPKFIHFEEQVKAEGIDLLLLEDVKNAF
                     DTTSFGQRYTEVLLKYDYIVGDWGNEQLRLKGFYKDSDDIKKTNRISRLEDYIKEFCN
                     FGCAYFVLENLHPQDIKFEEERQPRRKKSPKSKSNRRKPNYSNQQPATPKSKSKRASK
                     EKQPENQAFTSQKRRSNTKHKEKSKRNQTSQLNTKISHFIIRKKDK"
     gene            5788..6867
                     /gene="rlmN"
                     /locus_tag="ACGEUI_000548"
     CDS             5788..6867
                     /gene="rlmN"
                     /locus_tag="ACGEUI_000548"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFF8165406.1"
                     /translation="MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEE
                     MTNISKDFVSILNDSFCVNPLKQRVVQESADGTVKYLFELPDGMLIETVLMRQHYGHS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
                     GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVS
                     LHAPNNDLRSSIMRVNRSFPLEKLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQE
                     LADLTKTIRKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDVLKKNGVNCVVRQEHGT
                     DIDAACGQLRSKTMKKDREKVTATK"
     gene            complement(6949..7575)
                     /locus_tag="ACGEUI_000549"
     CDS             complement(6949..7575)
                     /locus_tag="ACGEUI_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFF8165407.1"
                     /translation="MIAFFSICSGILLTHLFYCLLLYFPDNGQEQLSKQTVIVCDQLT
                     FAKRVFRQFQNHFITSQKIFFSLEIGLIIILWASMVHWVSASYCYLLLFSLLFSLFDW
                     RSQEYPFILWLFSFVSLLLFYSINYLSLILLLLGLLAHLRPFSIGAGDFFYLASLALV
                     LDLTSLIWLIQLASLAGITACLLLGIKRIPFIPYLSFGLFWIVLLEHF"
     gene            7803..8330
                     /locus_tag="ACGEUI_000550"
     CDS             7803..8330
                     /locus_tag="ACGEUI_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HFF8165408.1"
                     /translation="MTNTLVENIYASVTHNISKKEASKNEKTKAVLNQAVADLSVAAS
                     IVHQVHWYMRGPGFLYLHPKMDELLDSLNANLDEMSERLITIGGAPYSTLAEFSKHSK
                     LDEAKGTYDKTVAQHLARLVEVYLYLSSLYQVGLDITDEEGDAGTNDLFTAAKTEAEK
                     TIWMLQAERGQGPAL"
     gene            8472..8675
                     /locus_tag="ACGEUI_000551"
     CDS             8472..8675
                     /locus_tag="ACGEUI_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFF8165409.1"
                     /translation="MKTLYDVQQLLKNFGIFVYLGKRLYDIEMMKIELQRLYDSGLLD
                     KRDYLNAELILRREHRLELEKEG"
     gene            8680..9651
                     /locus_tag="ACGEUI_000552"
     CDS             8680..9651
                     /locus_tag="ACGEUI_000552"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFF8165410.1"
                     /translation="MSQKLLGIDLGGTTIKFGILTAAGEVQEKWAIETNILEGGKHIV
                     PDIIASIKHRLDLYGLSSADFVGIGMGSPGAVDRDTNTVTGAFNLNWKETQEVGSVVE
                     KELGIPFAIDNDANVAALGERWVGAGENNPDVVFMTLGTGVGGGIIADGNLIHGVAGA
                     GGEIGHMIVEPENGFACTCGSHGCLETVASATGVVKVARLLAEAYEGDSAIKAAIDNG
                     EGVTSKDIFMAAEAGDSFADSVVEKVGYYLGLASANISNILNPDSVVIGGGVSAAGEF
                     LRSRIEKYFVTFTFPQVRYSTKIKIAELGNDAGIIGAASLARQFITK"
     gene            9661..10050
                     /locus_tag="ACGEUI_000553"
     CDS             9661..10050
                     /locus_tag="ACGEUI_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8165411.1"
                     /translation="MSPITLILWLLLVGIVGYYTWNYFSFRKMAKQVDNETFKDVMRQ
                     GQLIDLREPAAFRTKHILGARNFPAQQFDAAIKGLRKDKPVLIYENMRPQYRVPAVKK
                     LKKAGFEDVYVLKDGIDYWDGKVKQTT"
     gene            10233..12074
                     /gene="typA"
                     /locus_tag="ACGEUI_000554"
     CDS             10233..12074
                     /gene="typA"
                     /locus_tag="ACGEUI_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFF8165412.1"
                     /translation="MTNLRNDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELQER
                     AMDSNDLEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPAEVVDEVLELFIELGADDEQ
                     LEFPVVYASAINGTSSLSDDPADQEHTMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDCVEALPILRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSVTKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            12196..12447
                     /locus_tag="ACGEUI_000555"
     CDS             12196..12447
                     /locus_tag="ACGEUI_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HFF8165413.1"
                     /translation="MFYLIMAVSIISYYLYMAPKSVRNTLGMIGLVGLVALLIVLAGL
                     SFIKIMQTPPEFFIGMGMVALGYFALKDVRKMTKKPRVK"
     gene            12817..14175
                     /gene="murD"
                     /locus_tag="ACGEUI_000556"
     CDS             12817..14175
                     /gene="murD"
                     /locus_tag="ACGEUI_000556"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFF8165414.1"
                     /translation="MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFD
                     QNPAAQALLEEGIKVICGSHPVELLDENFEYMVKNPGIPYDNPMVKRALAKEIPILTE
                     VELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
                     GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESD
                     YLILNANQEISATLAKTTKATVIPFSTQKVVDGAYLKDGILYFKEQAIIAATDLGVPG
                     SHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQIKDITFYNDSKSTNI
                     LATQKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKA
                     EVSYLEARNVAEATELAFKLAQTGDTILLSPANASWDMYPNFEVRGDEFLATFDCLRG
                     DA"
     gene            14175..15257
                     /locus_tag="ACGEUI_000557"
     CDS             14175..15257
                     /locus_tag="ACGEUI_000557"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HFF8165415.1"
                     /translation="MPKKILFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     TEIEKSGLDVTFHAIATGKLRRYFSWQNLADVFKVALGLLQSLFIVAKLRPQALFSKG
                     GFVSVPPVVAAKLLGKPVFIHESDRSMGLANKIAYKFATTMYTTFEQEDQLSKVKHLG
                     AVTKVFKDANQMPESTQLEAVKEYFSRDLKTLLFIGGSAGAHVFNQFISDHPELKQRY
                     NIINITGDPHLNELSSHLYRVDYVTDLYQPLMAMADLVVTRGGSNTLFELLAMAKLHL
                     IVPLGKEASRGDQLENATYFEKRGYAKQLQEPDLTLHNFDQAMADLFEHQADYEATML
                     ATKEIQSPDFFYDLLRADISSAIKEK"
     gene            15258..16406
                     /locus_tag="ACGEUI_000558"
     CDS             15258..16406
                     /locus_tag="ACGEUI_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFF8165416.1"
                     /translation="MAKDKEKQSDDKLVLTEWQKRNIEFLKKKKQQAEEEKKLKEKLL
                     SDKKAQQQAQNASEAVELKTDEKTDSQEIESETTSKPKKTKKVRQPKEKSATQIAFQK
                     SLPVLLGALLLMAVSIFMITPYSKKKEFSVRGNHQTNLDELIKASKVKASDYWLTLLT
                     SPGQYERPILRTIPWVKSVHLSYQFPNHFLFNVIEFEIIAYAQVENGFQPILENGKRV
                     DKVRASELPKSFLILNLKDEKAIQQLVKQLTTLPKKLVKNIKSVSLANSKTTADLLLI
                     EMHDGNVVRVPQSQLTLKLPYYQKLKKNLENDSIVDMEVGIYTTTQEIENQPEVPLTP
                     EQNAADKEGDKPGEHQEQTDNDSETPANQSSPQQTPPSPETVLEQAHG"
     gene            16618..17982
                     /gene="ftsA"
                     /locus_tag="ACGEUI_000559"
     CDS             16618..17982
                     /gene="ftsA"
                     /locus_tag="ACGEUI_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFF8165417.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFISGEMNVIGVSNVPSTGVKD
                     GIIIDIEAAATAIKTAVEQAEEKAGMTIEKVNVGLPANLLQIEPTQGMIPVPSESKEI
                     KDEDVDSVVKSALTKSITPEREVISLVPEEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPSTILHNLRKTVERAGNKVENIIISPLAMAKTILNEGEREFGATVIDMGGGQTTVAS
                     MRAQELQYTNIYAEGGEYITKDISKVLKTSLAIAEALKFNFGQAEISEASITETVKVD
                     VVGSEEPVEVTERYLSEIISARIRHILDRVKQDLERGRLLDLPGGIVLIGGGAIMPGV
                     VEIAQEIFGVTVKLHVPNQVGIRNPMFSNVISLVEYVGMMSEVDVLAQTAVSGEELLR
                     RKPIDFSGQESYLPDYDDSRRPESTIGYEQQASQTAYDSQVPSDPKQKISERVRGIFG
                     SMFD"
     gene            18006..19325
                     /gene="ftsZ"
                     /locus_tag="ACGEUI_000560"
     CDS             18006..19325
                     /gene="ftsZ"
                     /locus_tag="ACGEUI_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFF8165418.1"
                     /translation="MAFSFDTASIQGAIIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEILTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVVTRPFGFEGNKRGNFAIEGIEELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITSPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERIVEAARKAIYSPLLETTIDGAQDVIVNVTGGLDMTLTEA
                     EEASEIVGQAAGQGVNIWLGTSIDDTMKDDIRVTVVATGVRQEKAEQVSGFRQPRTFT
                     QTNAQQVAGAQYASDQAKQSVQPGFDRRSNFDFDMGESREIPSAQKVISNHNQNQGSA
                     FGNWDLRRDNISRPTEGELDNHLNMSTFSANDDSDDELETPPFFKNR"
     gene            19328..19999
                     /locus_tag="ACGEUI_000561"
     CDS             19328..19999
                     /locus_tag="ACGEUI_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFF8165419.1"
                     /translation="MDLLTNKKKIFETIRLSTEAANRTNDSVSVIAVTKYVDSTIAGQ
                     LIEAGIEHIAENRVDKFLEKYDALKYMPVKWHLIGTLQRRKVKEVINYVDYFHALDSV
                     RLALEINKRADHPVKCFLQVNISKEESKHGFNISEIDEAIGEIGKMEKIQLVGLMTMA
                     PANASKESIITIFRQANQLRKNLQLKKRKNMPFTELSMGMSNDYPIAIQEGSTFIRIG
                     RAFFH"
     gene            20011..20667
                     /locus_tag="ACGEUI_000562"
     CDS             20011..20667
                     /locus_tag="ACGEUI_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFF8165420.1"
                     /translation="MAFKDTFNKMISYFDTDEVNEVEEDVAASTDNVIPRSQQSVRAS
                     SHPKQEPRNNHVQQDHQARSQEQTRSQMHPKHGTSERYYQQSQPKEGHEMVDRRKRMS
                     TSSIANRREQYQQSTCSDQTTIALKYPRKYEDAQEIVDLLIVNECVLIDFQFMLDAQA
                     RRCLDFIDGASKVLYGSLQKVGSSMYLLAPSNVSVNIEEMTIPHTTQDIGFDFDMKRR
                     "
     gene            20672..20926
                     /locus_tag="ACGEUI_000563"
     CDS             20672..20926
                     /locus_tag="ACGEUI_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFF8165421.1"
                     /translation="MILILSILLRLIKVYTYLLIAYALMSWFPGAYDSKIGRLISGIV
                     EPILKPFRAFNLQFAGLDFTIFVVIISLNFLAQVLVRVFI"
     gene            20926..21717
                     /locus_tag="ACGEUI_000564"
     CDS             20926..21717
                     /locus_tag="ACGEUI_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFF8165422.1"
                     /translation="MVSHSKIYQHFHQEEYPFIDRMSDMINRVEDYYLLEVTEFLNPR
                     EVMILKSLIALTDLKMFVSTDYYPSEYGRVIIAPGYYDLEQSDFQIALVEISYQAKFN
                     QLTHSQILGTLINELGVKRNLFGDVFVEMGYAQLMIKRELLDYFLGTITKIAKTSVKL
                     REVNFDQLIRSIDNSQTLDILVSSFRLDGVVATILKKSRTQVIALIEANKIKVNYRVA
                     NKASDNLVIGDMVSIRGHGRFTLLADNGVTKHGKQKITLSKMIHK"
     gene            21727..22485
                     /locus_tag="ACGEUI_000565"
     CDS             21727..22485
                     /locus_tag="ACGEUI_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFF8165423.1"
                     /translation="MALTTLEIKDKTFKTKFRGYCEEEVNEFLDIVVDDYEALVRKNR
                     DNEARIKDLEEKLSYFDEMKESLSQSVILAQETAEKVKATANAEATNLVSKATYDAQH
                     LLDESKAKANQMLRDATDEAKRVAIETEELKRQTRVFHQRLISSIESQLSLSNSPEWD
                     ELLQPTAIYLQNSDDAFKEVVKTVLNEDIPESDDSASFDATRQFTPEELEELQRRVDE
                     SNKELEAYQLDSQSDSTTEPEVNLSETQTFKLNI"
     gene            22758..25559
                     /gene="ileS"
                     /locus_tag="ACGEUI_000566"
     CDS             22758..25559
                     /gene="ileS"
                     /locus_tag="ACGEUI_000566"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFF8165424.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPNKEPQWQAAWEQAELYKKRQELN
                     AGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFQAPYVPGWDTHGLPIE
                     QVLAKQGIKRKEMDLAEYLEMCRQYALSQVDKQRDDFKRLGVSADWENPYVTLDPQFE
                     ADQIRVFGAMAEKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTNTYIVVWTTTPFTVTASRGLTVGPDMDYLVVKPAGSDRQYVVAEGLLDSLAGK
                     FGWESFETLASHKGADLEYIVTEHPWDTDVEELVILGDHVTLESGTGIVHTAPGFGED
                     DYNVGTKYKLEVAVTVDERGLMMENAGPDFHGQFYNKVTPIVIDKLGDLLLAQEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSDFRQDILDEIEKTTFHPSWGETRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFQENGSIIWWQKEAKDLLPEGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNTKENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAILSQGFVLDGKGEKMSKSKGNIISPNDVAKQYGADILRLWVAS
                     VDTDNDVRVSMEILGQVSETYRKIRNTLRFLIANTSDFNPATDTVAYADLGTVDKYMT
                     IVFNQLVATITDAYERYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSLERRR
                     MQTVFYDILVKITKLLTPILPHTTEEIWSYLEYESEAFVQLAEMPVAETFSAQEDILE
                     AWSAFMTLRTQAQKALEEARNAKIIGKSLEAHLTIYASEEVKTLLTALDSDIALLLIV
                     SQLTIADLADAPADAVAFEGVAFIVEHAIGEVCERSRRIDPTTRMRSYNAFVCDHSAK
                     IIEENFPEAVAEGFEESGK"
     gene            complement(25824..26126)
                     /locus_tag="ACGEUI_000567"
     CDS             complement(25824..26126)
                     /locus_tag="ACGEUI_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFF8165425.1"
                     /translation="MRLINTTSSHPELIKNQLKNTDAYLVEVYSAGNTDVIFTQAPKH
                     YELLISNKYRAIKEDELDIIREFFLKRKIDPKIVIPGQSKTLHTNNLIEISFQTSV"
     gene            complement(26177..26632)
                     /locus_tag="ACGEUI_000568"
     CDS             complement(26177..26632)
                     /locus_tag="ACGEUI_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFF8165426.1"
                     /translation="MMIPTFGHKNAHKDYVTRYGVYAIIPNHEQTKIILVQAPNGSWF
                     LPGGEIEAGEGQLQALERELIEELGFSATIGSYYGQADEYFYSRHRDTHFYHPAYLYE
                     VTAFQAVSKPLEDFNNLGWFSPIEAIAKLKRESHQWGVKEWQKKHHSTN"
     gene            complement(26760..29042)
                     /locus_tag="ACGEUI_000569"
     CDS             complement(26760..29042)
                     /locus_tag="ACGEUI_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8165427.1"
                     /translation="MLCQNCNLNESTIHLYTSVNGKQRQVDLCQNCYQIMKSDPANSI
                     LNGLTPGYRAQDRSTSPFFDDFFGDLNNFRAFGNLPNTPPTQAGQNGNGGGRYGGNYN
                     GQRPAQPQTPNQQAKGLLEEFGINVTDIARNGNIDPVIGRDEEITRVIEILNRRTKNN
                     PVLIGEPGVGKTAVVEGLAQKIIDGTVPQKLQGKQVIRLDVVSLVQGTGIRGQFEERM
                     QKLMEEIRNRKDVILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGELQLVGATTLN
                     EYRIIEKDAALERRMQPVKVDEPSVEETITILKGIQPKYEDYHHVKYSPAAIEAAAHL
                     SNRYIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDKRLIEAENLKAQATRDEDYE
                     RAAYFRDQITKYKEMQAQKVDEQDIPIITEKTIEAIVEQKTNIPVGDLKEKEQSQLVN
                     LANDLKAHVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLA
                     IELFGSTNNMIRFDMSEYMEKHAVAKLVGAPPGYIGYEEAGQLTEQVRRNPYSLILLD
                     EVEKAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKSEASVGFGAAR
                     EGRTSSVLGELSNFFSPEFMNRFDGIIEFKALSKEHLLHIVDLMLEDVNERLGYNGIH
                     LDVTQKVKEKLVDLGYDPKMGARPLRRTIQDYIEDAITDYYLEHPTEKQLRALMTNSE
                     NITIKAVKEGDSFLNEESLD"
     gene            29340..29570
                     /locus_tag="ACGEUI_000570"
     CDS             29340..29570
                     /locus_tag="ACGEUI_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFF8165428.1"
                     /translation="MESHLVRIINRLELMVTDGGNLKRNFERDGVVVAEVAFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            29697..30383
                     /locus_tag="ACGEUI_000571"
     CDS             29697..30383
                     /locus_tag="ACGEUI_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8165429.1"
                     /translation="MDLSFLPKYWAYFNYGVLVTIMISVSVVFFGTLIGVLVTLIKRS
                     HVKPLTWVVNLYVWIFRGTPMVVQIMIAFAWMHFNNMPTIGFGVLDLDFSRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPKGQLEAAYSLGIRPQNAMRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPISPLLVAAFYYLMVTTVMAQLLAVL
                     ERHMAQGGNH"
     gene            30383..31117
                     /locus_tag="ACGEUI_000572"
     CDS             30383..31117
                     /locus_tag="ACGEUI_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8165430.1"
                     /translation="MTEAIISIKDLHKYYGHNEVLKGIDLDIMPGEVVVIIGPSGSGK
                     STLLRTMNLLEVPTKGQIRFEGIDITDKKNDIFSMREKMGMVFQQFNLFPNMTILENI
                     TLSPIKTKGMAKAEADKTALSLLDKVGLSEKAKAYPASLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAKEVADRVMFMDGGVI
                     VEEGSPNQLFDLTKEERTKDFLSRVL"
     gene            31266..32675
                     /locus_tag="ACGEUI_000573"
     CDS             31266..32675
                     /locus_tag="ACGEUI_000573"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFF8165431.1"
                     /translation="MVSVMWFRRDLRIDDQKALAHAIASQSPTLCVFQFNKEQLNLLH
                     SRNQSSFITSVLAFRKLLKCEGIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGY
                     GRLRDQKAAQFFKKNGIAVHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKET
                     PIKVELSQGRWLNLETPDDVLRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHA
                     NRDFPAQLGTSRLSPFLRIGAIGIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYV
                     AYPDQKTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRM
                     IVASFLTKDLLCDWRLGEQYFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRIFNPVT
                     QGKRFDPKGEFIKAYLPQLEHVPEKYLHEPWKMPKNLQESVSCIIGTDYPQPIVDHAK
                     QREQAIAKYEWAKEKAKIE"
     gene            32853..34547
                     /locus_tag="ACGEUI_000574"
     CDS             32853..34547
                     /locus_tag="ACGEUI_000574"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFF8165432.1"
                     /translation="MTYNEVYQEWLHNNDLSDDIKADLAAIKDNEAEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAVKKGIAVSYDVRYQSRTFA
                     ELTCSIMAANGIKAYLYKGIRPTPMCSYAIRALGCISGVMITASHNPQAYNGYKAYWQ
                     EGSQILDDIADQIAQHMAALTQYQEIKQMPFEKALDSGLVTYIDESIEEAYKKEVLGL
                     TINDTDIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPN
                     PEVPKTFAYSEKLGKAVDADILIATDPDCDRVALEVKNAVGDYVFLNGNKIGALLSYY
                     IFSQRFDLGNLPANPVLVKSIVTGDLSRAIASHYGIETVETLTGFKNICGKANEYDVT
                     KQKNYLFGYEESIGFCYGTFVRDKDAVSASMMIVEMAAYYKKKGQNLLDVLQTIYATF
                     GYYNERQIALELEGIEGQKRIARIMEDFRQTPIASVAEMALDKTIDFIDGYQDFPKQN
                     CLKFYLDDGSWYALRPSGTEPKIKFYLYTIGQTQENSATKLDAIEAACRTKINQVN"
     gene            34755..35609
                     /locus_tag="ACGEUI_000575"
     CDS             34755..35609
                     /locus_tag="ACGEUI_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFF8165433.1"
                     /translation="MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDP
                     ASQVYVRNKERAALTVGFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPN
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQ
                     YIKDGAIVIDVGMNRDDNGKLIGDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQ
                     SALRSTHK"
     gene            35762..37102
                     /gene="xseA"
                     /locus_tag="ACGEUI_000576"
     CDS             35762..37102
                     /gene="xseA"
                     /locus_tag="ACGEUI_000576"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFF8165434.1"
                     /translation="MADYLTVTHLTKYLKLKFDRDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDESAVIQATMWAGVYKKLGFDLEEGMKINVIGRVQLYEPSGSYSIVIEKAEPDG
                     IGALALQFEQLKKKLTAEGYFEQKHKQPLPQFVSKIGVITSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGDGAAQEVVANIRRANQREDLDLLIVGRGGGSIEDLWAFNEEIVV
                     QAIFESQLPVISSVGHETDTTLADFVADRRAATPTAAAELATPITKTDLMSWIVERQN
                     RSYQACLRRIKQRQEWVDKLSQSVIFRQPERLYDAYLQKIDRLSMTLMNTMKDRLSSA
                     KENKVQLDHALANSQLQTKIERYQDRVATAKRLLMANMASQYDSQLARFEKAQDALLS
                     LDASRIIARGYAMIEKNQALVASVSQITKGDQLTIKMRDGQLDVEVKDVKNENI"
     gene            37080..37295
                     /locus_tag="ACGEUI_000577"
     CDS             37080..37295
                     /locus_tag="ACGEUI_000577"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFF8165435.1"
                     /translation="MSKTKTFEENLQDLETIVNKLENGDVPLEEAISEFQKGMLLSKE
                     LQKTLQAAEKTLVKVMQADGTEVDMDD"
     gene            37295..38167
                     /locus_tag="ACGEUI_000578"
     CDS             37295..38167
                     /locus_tag="ACGEUI_000578"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFF8165436.1"
                     /translation="MDKLARIDEAIRRYYKTTSNGVSEELIDAILYSVDSGGKRIRPL
                     ILLEMIEGFGVSLQNAHFDLAAALEMIHTGSLIHDDLPAMDNDDYRRGRLTNHKQFGE
                     ATAILAGDSLFLDPFGLIAQAELNSEVKVALIQELSLASGTFGMVGGQMLDMKGENQA
                     LSLPQLSLIHLNKTGKLLAFPFKAAALITEQAMTVRQQLEQAGMLIGHAFQIRDDILD
                     VTASFEDLGKTPKKDLFAEKATYPSLLGLEASYQLLTESLDQALTIFQTLESDVGFKP
                     QIITKLIEGLRLNA"
     gene            38160..38987
                     /locus_tag="ACGEUI_000579"
     CDS             38160..38987
                     /locus_tag="ACGEUI_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HFF8165437.1"
                     /translation="MPKERVDVLAYKQGLFETREQAKRGVMAGLVVSVINGQRYDKPG
                     DKIDDGTELKLKGEKLKYVSRGGLKLEKGLHVFGVSVANQIGIDIGASTGGFTDVMLQ
                     DGAKLVYAVDVGTNQLVWKLRQDPRVRSMEQYNFRYAQPEDFNEGQPVFASIDVSFIS
                     LSLILPALHNVLSDQGQVIALIKPQFEAGREQIGKKGIVKDKQIHEKVIQKVMDFASG
                     YGFTVKGLDFSPIQGGHGNIEFLAHLAKSQTPETLAPHLIQKVVAKAHKEFEKHEKE"
     gene            38974..39444
                     /locus_tag="ACGEUI_000580"
     CDS             38974..39444
                     /locus_tag="ACGEUI_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8165438.1"
                     /translation="MKKSERLELIKKMVLTHPIETQHDLLRLLAEHGLELTQATISRD
                     MNEIGIVKIPSGSGRYIYGLSQDSGKKIVQGPRSIKSTILAVSDKTKGLEQHLYLKVV
                     PGNSKLIKRYLLADFSKAIFSLIADDDSLLLIAKSPSEADMIRQEILLWMQGIT"
     gene            39466..41127
                     /gene="recN"
                     /locus_tag="ACGEUI_000581"
     CDS             39466..41127
                     /gene="recN"
                     /locus_tag="ACGEUI_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFF8165439.1"
                     /translation="MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNM
                     MLGARASTEVIRRGANKAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGR
                     SVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
                     YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDR
                     LMNHKQIADTLTNAYVMLDNDDFSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYY
                     ILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDILNSLTRKYGGNVNDVLDYYDNIVKEY
                     QLLTGDDLSSGDLEAELKSLEKQLVAAASELSVSRHQLAEQLEAEIKAELKELYMEKA
                     DFKVHFTTSKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIYKIGRHGQVLAISHLPQVIAIADYQYFISKES
                     KEESTVSKVRLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH"
     gene            41299..42240
                     /locus_tag="ACGEUI_000582"
     CDS             41299..42240
                     /locus_tag="ACGEUI_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8165440.1"
                     /translation="MTSKKACLSSIIVLASLTCGNDTVSANHLSATGDKFDDCSTLVE
                     KDVAPKDELEMLAWSSSQTTDDADRDYEDFLDDDSFISQNETDKMFENLTDDRLLNEL
                     DELDEENEEDEEDTIEPEQNVIMPSDDELFDLTDAVETRLTVSSAPHLEAELPKPHLR
                     SLSDTALRSGEIRGHLDNKLDALSVTATKLALTMAQKFDLTTHVYSIGESFSEVLAAH
                     YEDRKAESAFSKKKRFHLPIATPDVVIEELRRLVSSIGSSKEDVSVPYSRKLGMAVAK
                     RKIALPQTGEGFSYYPVLLGLMILGLTPIMIPKKINN"
     gene            42468..43307
                     /locus_tag="ACGEUI_000583"
     CDS             42468..43307
                     /locus_tag="ACGEUI_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8165441.1"
                     /translation="MGTIKIVTDSSITIEPELIKALDITVVPLSVMIDSKLYSDNDLK
                     EEGHFLSLMKASKSLPKTSQPPVGLFAETYENLVKKGVTDIVAIHLSPALSGTIEASR
                     QGAEIAEAPVTVLDSGFTDQAMKFQVVEAAKMAKAGASLNEILAAVQAIKSKTELYIG
                     VSTLENLVKGGRIGRVTGVLSSLLNVKVVMALKNDELKTLVKGRGNKTFTKWLDSYLA
                     KNSHRPIAEIAISYAGEASLALTLKERIAAYYNHSISVLETGSIIQTHTGEGAFAVMV
                     RYE"
     gene            43300..44142
                     /locus_tag="ACGEUI_000584"
     CDS             43300..44142
                     /locus_tag="ACGEUI_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFF8165442.1"
                     /translation="MNNRHLFSGIFFFVISLCLAFLLLNIIIPKSNSRLKKSDFLKKE
                     QVAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQIL
                     DRMIKQKQIQLSLKKADIMTLTVGGNDVMAVIRKNLADLQVSSFRKPARQYQKRLRQI
                     IELARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNTKTKEVVGEYDRVYFVPI
                     NDLLYKGINGQEGIVHSSGDQTTIVNDALFTGDHFHPNNTGYQIMSNAVMEKIKKHEK
                     KIKP"
     gene            44120..44707
                     /locus_tag="ACGEUI_000585"
     CDS             44120..44707
                     /locus_tag="ACGEUI_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFF8165443.1"
                     /translation="MKKKSNLNWWKWSFLCLLAFNTAFLMVIASRLIQVREPESELIA
                     KKPVKNIKIGTFVTTREQLNETVASYLKDYQTEKMSYKFYATSSSILFEGTYQLLGYE
                     VPLYIYFQPHRLENGAVQLQVISFSVGTLPLPEKDVLQYLKSSYKLPSFVKVMPNQSA
                     IVVNLQDIQNDAKVYLKAKKIDLFNDEISFNIYKK"
     gene            44806..45081
                     /locus_tag="ACGEUI_000586"
     CDS             44806..45081
                     /locus_tag="ACGEUI_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFF8165444.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFSTIEAFLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            complement(45171..46313)
                     /locus_tag="ACGEUI_000587"
     CDS             complement(45171..46313)
                     /locus_tag="ACGEUI_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8165445.1"
                     /translation="MWIEETDNGKFKFRENYKDPYTGTWKPVSVTMEKDNSRAYKAAR
                     KILEQKITEKIAQLKASELLFTELLDEWWAFYKKELKRSSVASLRGNIEEIRETFGIG
                     VKVVNIDPKYVQNYLDNLDCSRNKKERNKSMLNLAFDYAVGLDIIQDNPARRAKLPRV
                     KKTLEDWKKAEEKYLEEDEIKPLLKELYRRPSTYRLGLLAEFISLNGCRIGEAVSIEP
                     CNYESKSRILQLHGTFDHTEGYRNGEKTAPKTLASYRETIMTSRELEILQELEFMNEL
                     EKNTNHRYRDMGYLFTTKNGVPIQTNSFNLALKKANERLEDPITKKLTSHIFRHTLIS
                     RLAENNVPLKSIMERVGHADAKTTAQIYTHVTKKMKSSVADIMENY"
     gene            complement(46437..46703)
                     /locus_tag="ACGEUI_000588"
     CDS             complement(46437..46703)
                     /locus_tag="ACGEUI_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFF8165446.1"
                     /translation="MGFLDNVKEASTVSTASGENYEYVVLQVTLKEKLIGTGSGNLTE
                     LENVINKQAKKGYRLHTITTSNGGSKGFGGGDRIQATMVFEKIK"
     gene            complement(46715..47095)
                     /locus_tag="ACGEUI_000589"
     CDS             complement(46715..47095)
                     /locus_tag="ACGEUI_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFF8165447.1"
                     /translation="MTIEEILDFHNVELAYFDNELWPRPGIYIDEIKVVFVNRALSDE
                     SKKKVIFHELGHIEHDSGQYQRRHEEFELQANRHMIRCLLEDEFDKVEDKHEFNYLSF
                     MQRHNLKTTTDEVMVIDEYYTLIG"
     gene            complement(47099..47446)
                     /locus_tag="ACGEUI_000590"
     CDS             complement(47099..47446)
                     /locus_tag="ACGEUI_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8165448.1"
                     /translation="MFITFERIKELAKKQGLSINSLEEKMGYSRNTIYALKRNKPGSE
                     KLQEIADYFHVSTDYLLGRTDNPAIAKDNQEYTSEDLRKMAKNAKTFDGKPLTESDIE
                     AIQNIIEIYLKGR"
BASE COUNT        15155 a         8117 c         9891 g        14496 t
ORIGIN      
        1 attgagaatt agccttttct tttagctttg ataggtgtat aataaccata ctatttttta
       61 tttttcaaaa tattagaata aacaaaagtg gacaacgtat aaagagaact gctaagtcgt
      121 tgagctcatt agtcccaaaa agattaccaa aaaagaagga agaactatga agaaaagttt
      181 tattcatcaa caagaggaga tttcctttgt taagaatacg ttcactcagt atttgattgc
      241 taagttagat gttgttgaag tgcaaggacc cattttaagt cgggtcgggg acggtatgca
      301 agataatttg tccggtactg aaaatccagt atctgtcaat gtcttgaaaa ttcctaatgc
      361 aacctttgaa gttgtgcatt cattagcaaa atggaaacgt cacaccttag cccgttttgg
      421 gtttaatgaa ggtgaaggcc ttgttgttaa tatgaaggct ctccgacctg atgaggattc
      481 acttgaccaa acccattctg tctatgttga ccaatgggac tgggaaaaag tcatccctga
      541 tggcaaacgt aatctcgctt acctcaaaga aaccgttgaa accatttaca aggtcattcg
      601 gttaactgaa ttagctgtag aagctcgtta tgatattgag gctgttttac ctaagaaaat
      661 aaccttcatt cataccgaag aattggtagc taaataccct gatttgacgc ccaaagaacg
      721 tgaaaatgcc attactaaag aatttggagc agtcttttta attggtattg gcggggtttt
      781 acctgacggt aaaccacacg atggccgcgc tcctgactat gatgactgga caacagaaac
      841 tgaaaatggc tatcatgggc ttaatggtga tattttggtt tggaatgacc aacttggttc
      901 agcctttgaa ttatcctcaa tggggatccg tgtggatgaa gaggccctaa aacgtcaggt
      961 ggaaatgact ggtgaccaag accgcttagg ctttgattgg cacaaatctt tgttgaatgg
     1021 acttttccct ctaacgattg gtggaggaat cggtcaatca cgtatggtca tgtttttgtt
     1081 gcgtaaacaa catattggag aagttcagac ctccgtttgg ccccaagaag ttcgtgatag
     1141 ctacgataat attttataag taatcacatc attttgctaa gttttccact tgtataagag
     1201 actttaaata aatgatcaga aatattgaaa aaagaaatca tttttaaggt ttagagctag
     1261 aaatttgcta taataaatgt tatgagagtt gtatcaggtg aatttggtgg gcgtccttta
     1321 aagacgctcg atggaaagat aacacgccct acttcagaca aagttagagg tgcgattttt
     1381 aatatgatag gaccttactt taatggtggt cgtgttttag atttgtttgc aggttcaggt
     1441 ggattagcca ttgaggctgt ttctcgtggg atgtctgctg cagttttggt tgagaagaat
     1501 cgtaaagcgc aagctattat tcaagacaat attataatga ctaaagctga gaatcggttt
     1561 accttgttaa aaatggaagc tgagcgagct attgattgct tgactggtcg atttgactta
     1621 gtctttttag accctcctta tgccaaagaa acaattgtgg caactattga agctttagca
     1681 gctaaaaact tattgagtga acaggttatg gttgtctgtg agacagataa aacagtcttg
     1741 ttgccaaaag agattgcaac gttaggtatt tggaaagaaa aaatttatgg aattagtaag
     1801 gtaacagttt atgttaacta aaataggact ttatacaggg tcatttgatc ctgttacaaa
     1861 cggtcatctc gatattgtta aacgggcaag tggcctattt gatcaaatat atgttgggat
     1921 ttttgataat cctaccaaaa aaagctattt taaactagaa gttcgtaaag caatgcttac
     1981 tcaagctctg gctgatttta caaatgtaat tgtagtaact tcgcacgaac gtttagctat
     2041 tgatgtagct aaagaattac gagtaactca cttgattaga ggcttgcgaa atgcaactga
     2101 ctttgagtat gaagaaaatt tggaatattt taatcacctt ttagctccaa atattgaaac
     2161 cgtctatttg atatcaagaa ataaatggca ggcccttagc tctagtcgtg tgagagagct
     2221 aattcatttt cagtcttcct tagaaggttt ggttcctcag tcggttattg ctcaagtgga
     2281 gaaaatgaat gaaaagactt aaaaaaatca aatggtggtt agtgggtctg ctagctttaa
     2341 tctctttgtt gctagcgtta ttttttccgc taccttatta tattgaaatg cctggaggcg
     2401 cttacgatat tcggactgtc ttacaagtca atggcaaaga agacaaacga aaaggagctt
     2461 accagtttgt tgcagtgggc attagtcgtg ccagcctcgc tcagctatta tatgcttggc
     2521 tgacaccgtt tactgaaatt agtacagcag aagatacaac aggcggatac agcgatgctg
     2581 atttccttcg aattaatcaa ttttacatgg aaacatcaca aaatgcagct atttatcaag
     2641 ctttatcctt agctggaaaa ccagttacat tagattataa aggcgtatat gttttagacg
     2701 taaacaacga atctactttt aaaggaacgc tacacttagc agatactgta acaggtgtaa
     2761 atggtaaaca gtttactagt tcagcagaac ttattgacta tgtttctcac ctaaaactag
     2821 gggatgaagt tacggttcag tttacgagtg ataataagcc taaaaaagga gttggccgta
     2881 ttatcaaact gaaaaatggg aaaaatggga ttggcattgc cttgactgat catacaagtg
     2941 tcaattcaga agacacagtg atctttagta ctaaaggagt aggaggacct agtgctggtc
     3001 taatgtttac tcttgatata tatgatcaaa taactaaaga agatttacgc aagggccgta
     3061 caattgcagg tacaggaact attggcaagg atggcgaagt aggagatatt ggtggtgcag
     3121 gtcttaaagt agttgcagca gctgaagctg gtgcagatat attttttgtt ccgaataatc
     3181 ctgttgataa ggaaattaaa aaagttaatc caaatgctat aagtaattac gaagaagcca
     3241 aacgggcagc caaacgacta aagaccaaaa tgaagattgt tcctgttacg actgttcaag
     3301 aggcactggt ttatcttcgc aaataaaata actatgaatg tattatattc aattatattc
     3361 acgctattaa ctcataaaaa acggttgaac cagatagaca gatccttgac cagtcttttg
     3421 ggttcaatca ttttttacat aaaacatatc taacttgaca gcgtaaaaaa gatgttataa
     3481 aagtctagtg gtaaaaaggc aatagagaat tgagtaaact ttagtcgatt atctggaaat
     3541 agagcatact aaaaacacct agtgaaaatt gatttttttc actagctcat ccaagaactt
     3601 gcgcagataa tcatgagtcc attccatgac tctattattc tggaaagcct tgcaatagac
     3661 aagttaacgt tataacaact aaattaaaaa cttgagattg aaaaatcccc ataaacttat
     3721 tttacaagga ggtaaggcta tggtaaccat taaacaagtt gctgaagaag caggtgtttc
     3781 tagatcaaca gtctccaggt atatttctca aaaaggatat gtatctgatg atgcaaggca
     3841 taaaatcaaa gcagcgatag caaaattaca ttacactcca aatgtcttgg cgcagtcatt
     3901 gaagacgaaa aaaatcaact agtcggttta ttattaccag acatttccaa tccttttttt
     3961 ccgaggttag caagaggagc agaagaatat ttaaaagaaa aggggtatcg ggttatgcta
     4021 ggaaatattt cagacagcga agctcttgaa gaagaatacg tccatgtcct tcttcaaagc
     4081 aatgctgctg gcattattac tacccatgac tttacaaagc ggtatcctac tcttgctatt
     4141 ccagttgtag tggtagatcg tgttgaccaa gagacccagt acggtgtctt ttcagataat
     4201 agggcagggg gcttattggc ggctcagact gtctggcaag caggggcaaa agaagttttg
     4261 ttaataagag gtccgctgga taatgctgaa aacattaatg agcggtttga agctagtttt
     4321 tcttaccttc aaaaacaaga cgtgactatg tatgtgtgtg atagtcagaa ttttgatttt
     4381 gaaagtattc agctagaagc tagttacaac ctcaagtgtt acccaacaat agacagtatt
     4441 atagcacctt ctgatatcca tgctattgct tatattcatg aactgcattc tcaaggtaaa
     4501 aagattcctc aagatgttca aataataggt tatgatgaca ttttaatgag tcaatttatt
     4561 tatccttctt tatcgactat tcatcaatcc tcttatttga tgggccgtta tgccgccgaa
     4621 ttagtttata ctattgctag tcaattaact gtgaaagcaa atcggattaa attgcctgtt
     4681 cactacgttg agcgagaaac aataaggaaa agataagtaa taatattagt ccagatttta
     4741 gcaggtatga tgaagattta ttgggattta ccttctactt taaaagaagt tatctgtcat
     4801 tactttcatc gctatcagtc aaaatgcatt cttatgcaac tcttaacctt aagcttaatg
     4861 tctttttgat aacatagagg aatgttacac gatgatgatg caataaagcc taaatatagt
     4921 aattgtcgtg attttatagc tagtgagtaa tgagatagca agtctctcat ttacttacaa
     4981 aagtcaaaaa atagtgtata atggttgagt tacagataac actgcaaaaa agataacaag
     5041 tgtccataat tatctaagac tataaaggcg atatagacag atataaaaaa gatgacgaat
     5101 cagttatcac tggacaatta accgatttag ttacaactaa tggatttttc agaaaggtgt
     5161 caaatgaaaa aagaaatttc gccagaaatg tacaactata ataaatttcc aggtcccaaa
     5221 tttattcatt ttgaagagca agttaaggct gaaggcattg atttgttact cttagaagat
     5281 gttaagaacg cttttgatac gactagcttt ggtcaacgtt atacagaagt tctcctaaag
     5341 tatgactata ttgttggcga ttggggaaat gaacagcttc gtcttaaagg gttttataag
     5401 gatagtgacg atatcaagaa aacgaatcgc atctcacgtt tagaagatta tattaaagaa
     5461 ttttgcaatt ttggttgtgc ttattttgtt ttagaaaatc ttcatccaca agatattaaa
     5521 tttgaagagg agcgccaacc aagacgaaag aaatcaccta aatcaaaatc aaatcgtcgc
     5581 aagccaaact attcaaatca gcagcccgca acacctaaga gcaaatcgaa gcgggcatca
     5641 aaagaaaagc aacctgaaaa ccaagcgttt accagtcaaa aacgtcgaag taatactaag
     5701 cataaagaaa agtcaaaacg taatcagact agtcaactta ataccaaaat tagtcatttt
     5761 atcatcagaa agaaagataa ataatccatg aaaccatcga tttatagctt aacacgcgat
     5821 gaactgatcg cgtgggcagt tgaacgcggc caaaaacagt ttagagctac tcaaatttgg
     5881 gattggctct ataaaaaaag agttcaatct tttgaggaga tgacgaatat ttctaaagat
     5941 tttgtttcta ttttaaatga tagtttctgt gtcaatcctt taaaacagcg agttgtacaa
     6001 gagtcagctg atggtacagt aaaatattta tttgaactgc ccgatggtat gttaattgaa
     6061 acagttttaa tgcgtcaaca ttatggtcat tctgtttgtg tgacgactca agttggctgt
     6121 aacattggct gtactttttg tgctagtgga ttgatcaaaa agcaacgtga tctaaacagt
     6181 ggggaaatta cagctcaaat catgttagtc caaaaatatt ttgacgatag aaagcaaggt
     6241 gagcgggtta gccatgttgt cgtaatgggc attggtgaac cttttgataa ttacaaaaat
     6301 gtcatgtgct ttcttcgcgt catcaacgat gataatggct tagcaattgg tgcccgtcat
     6361 attacagtct ctacctcagg gttagctcac aagattcggg attttgcaaa cgagggagta
     6421 caggttaact tagcagtctc gttgcatgca ccaaataatg atttacgttc aagtattatg
     6481 cgagtaaatc gctcttttcc gcttgaaaag ctcttttcag ctatagaata ctatattgaa
     6541 aaaacgaatc gtcgtgtcac ttttgaatac attatgctaa atgaagttaa cgatagcatt
     6601 aagcaagctc aagaattagc agacttaact aaaactattc gtaagttatc ttatgttaac
     6661 ttgattccgt ataatcctgt ttcagaacat gatcagtata gtagaagtcc taaagagcga
     6721 gttctagcgt tttatgatgt gctaaagaaa aatggagtta actgtgtggt gcgtcaagag
     6781 cacggaacag atattgatgc agcctgtggc cagttacgtt ctaaaacgat gaaaaaagat
     6841 cgcgaaaagg ttacagcgac aaaataaagc ccaaaaaata tctttgattg acaatgcttg
     6901 atgacaaaat attcctagtc actaaaatga ctaggttttt tatctggcct aaaaatgctc
     6961 tagaagcaca atccaaaata atccaaaact aagataagga ataaaaggta tgcgcttgat
     7021 gcctaatagt aagcaagcag taatacctgc taaactagca agttgaatta gccaaatcaa
     7081 cgacgttaga tccaatacta aagctaagct agcaaggtag aaaaaatcgc ctgctccaat
     7141 tgaaaaaggt cttaaatgag ctaataaacc tagtagcagt aatatcaatg ataaataatt
     7201 gatcgaataa aacaggagta gacttacaaa gctaaaaagc cacaagataa aagggtattc
     7261 ttgagaacgc caatcaaata aactaaataa taaagaaaag agtaacaagt aacagtaact
     7321 agcactaacc caatgtacca tactagccca taagataatt attagaccta tctctaagct
     7381 aaagaagatt ttttggctag ttataaaatg gttttggaac tgccgaaaaa cgcgtttggc
     7441 aaaagttagt tggtcacaaa caatgacagt ctgtttactt agctgctctt gaccattatc
     7501 aggaaaataa agtagtaaac agtaaaaaag atgggtcaat aagatgcccg aacatatact
     7561 aaaaaaagct atcatactta tttattcgaa aagaaatcaa atacttgcaa tctttcctta
     7621 atcattgtag aatatagata taaaacgata atgattctaa ttaagtgtct aatcatccaa
     7681 ttaggattat tccgttattg tcgaatagga ttaggtcctt aaccctttct tatgtttatc
     7741 acgttagggt tagttccctc tccattcttg ccctttaagc tgaaaagtaa ggagatgtta
     7801 ttatgacaaa cacactcgtt gaaaatattt atgcttcagt aactcacaat atttctaaaa
     7861 aagaagcatc aaaaaatgaa aaaactaaag ctgttttaaa ccaagctgtc gctgacttat
     7921 ctgtggctgc atctattgtg caccaagtac actggtatat gcgtggtcca ggtttcttgt
     7981 accttcaccc caaaatggat gaattacttg atagcttgaa tgctaattta gatgaaatga
     8041 gcgagcgttt gatcactatt ggcggagcgc cttattctac cctagctgag ttttctaaac
     8101 actctaaatt ggacgaagca aaaggtactt acgataaaac cgttgctcag catttagcaa
     8161 gattggtcga agtttacctt tacctcagca gcctttacca agttggtttg gatattaccg
     8221 atgaagaagg cgatgctgga acaaacgatt tgtttacagc agctaagaca gaggctgaaa
     8281 aaaccatttg gatgctccaa gcagagcgtg gacaaggccc agcactctaa ttgattatgc
     8341 cttaggataa aagaagtcaa tgataaaatt ggcttctttt ttatggaatc atcgattcat
     8401 tttcagaaac atagaaagcg attccatact tgtcttgaaa tctgccgtag aatttgctaa
     8461 aatctactta tatgaaaaca ctatatgatg tgcaacaatt gctaaaaaac tttggcattt
     8521 ttgtttacct tggcaaacgt ctttatgata ttgaaatgat gaagattgaa cttcagcgtc
     8581 tctatgatag cggcttattg gataagaggg attacttaaa tgcggagtta attttgcgac
     8641 gtgagcatag attagaattg gaaaaagaag gatagacaaa tgagtcaaaa attactgggg
     8701 attgatttag gtggtaccac cattaaattt ggaattttaa cagcagcagg agaagtccaa
     8761 gaaaaatggg caattgagac taatatttta gaaggaggca aacatattgt ccctgatatc
     8821 atcgcttcca tcaaacatcg cttagacttg tatggtctaa gcagtgctga ctttgtggga
     8881 attggtatgg gatcaccagg agcggttgac cgtgatacta atacagttac tggggcattc
     8941 aaccttaatt ggaaagaaac ccaagaagtc ggttccgttg ttgaaaaaga attgggcatt
     9001 ccttttgcca ttgacaatga tgccaatgtg gctgcccttg gtgaacgttg ggtaggtgct
     9061 ggtgaaaata acccagatgt cgtcttcatg acacttggaa caggtgtcgg tggaggcatt
     9121 attgctgatg gtaacttgat tcatggtgtt gcaggagcag gtggtgaaat cggccacatg
     9181 attgttgagc cagaaaatgg ctttgcttgt acttgtggct cacacggctg tttggaaaca
     9241 gtagcttcag caacaggagt tgtcaaagtg gcacgtttac tggcagaagc ctacgaaggg
     9301 gattcagcca tcaaagcagc tattgacaat ggtgaaggtg ttaccagtaa agacattttc
     9361 atggcggctg aagcagggga ttcctttgct gattctgttg tggaaaaggt tggttactac
     9421 cttggccttg cttcagcaaa tatttccaat atcttgaatc cagactcagt ggttatcggt
     9481 gggggtgtgt cagcagcagg agaatttcta cgctcacgta ttgaaaaata ctttgtgacc
     9541 ttcactttcc cacaagtgag gtattcaact aaaattaaga ttgcagaact tggcaatgat
     9601 gctggaatta tcggtgcagc tagccttgct cgacaattta tcacaaaata ggagggggat
     9661 atgtctccaa tcacacttat cttatggtta cttcttgtcg gcattgttgg ttattataca
     9721 tggaactatt tctcttttcg aaaaatggca aagcaggttg ataacgagac ttttaaagac
     9781 gtgatgcgtc aaggtcaatt gattgattta cgagagccag cggcttttag aactaagcat
     9841 attttaggag ctcgaaattt tccagcgcaa caatttgatg ctgctattaa gggactacgc
     9901 aaggacaaac ctgttcttat ttacgaaaat atgcgtcccc agtaccgtgt gcctgcggtc
     9961 aaaaaactta aaaaagctgg ctttgaagac gtttatgtct taaaagacgg tattgattat
    10021 tgggatggca aagttaaaca aacaacctag ttaagagtaa cgtggagtta aggggaataa
    10081 aggcagtcac tgtctcaaaa accttaattc ctttttttgc tgtatccaga cttgctgaaa
    10141 gtctgaaaac atttacaatt gattaaaacc agttttttaa aacattttgt gttatactta
    10201 tctagttaaa atatatttac ttagaggaac aaatgactaa cttaagaaac gatatccgta
    10261 acgtagcgat tattgcccac gttgaccacg gaaaaacaac acttgtagat gaattattaa
    10321 aacaatccca tactcttgat gagcgtaaag agcttcaaga gcgtgccatg gattccaatg
    10381 accttgaaaa agaacgtggg attacaatcc ttgcgaaaaa tacggcagta gcctataacg
    10441 atgttcgtat taacatcatg gataccccag gacacgcgga cttcggtggt gaagttgaac
    10501 gtatcatgaa aatggttgac ggggttgttc ttgttgtgga tgcctacgaa ggaacaatgc
    10561 cccagacgcg tttcgtattg aaaaaagcac ttgagcaaaa ccttatcccg atcgttgtgg
    10621 tgaacaagat tgacaaacct tcagctcgtc cagcagaagt tgtagatgaa gtgcttgaat
    10681 tattcatcga acttggtgcc gatgatgagc aattggaatt cccagttgtt tacgcatcag
    10741 ctattaatgg aacatcatca ttatcagatg accctgctga ccaagagcat actatggcac
    10801 cgatctttga tacgattatt gatcatattc cagcgccagt tgataattca gatgagcctt
    10861 tgcaattcca agtgtcactt ttggactaca acgatttcgt aggtcgtatc ggtatcggtc
    10921 gtgttttccg tggtactgtt aaagtgggtg accaagtaac tctttcaaaa cttgatggta
    10981 ccactaaaaa cttccgtgtt acaaaactgt ttggtttctt cggtttggaa cgtcgtgaaa
    11041 ttcaagaagc taaagcaggt gacttgattg ctgtttcagg tatggaagat atctttgttg
    11101 gagaaaccat tacaccaact gactgtgtgg aagctctgcc aattcttcgt attgatgagc
    11161 caacacttca gatgactttc ttggtcaata actctccttt tgcaggtcgt gaaggtaaat
    11221 ggatcacgtc acgtaaggtt gaagaacgtc ttttagcaga attgcaaaca gacgtgtcac
    11281 ttcgtgttga cccaacagat tcgccagata aatggacggt ttcagggcgt ggagaattgc
    11341 atttatctat cctcattgaa accatgcgcc gtgaaggcta tgaacttcaa gtatcacgtc
    11401 cagaagttat catcaaagaa attgatggtg tcaaatgtga accgtttgag cgtgttcaaa
    11461 ttgatacacc agaagaatat cagggtgcaa tcatccagtc tctttcagaa cgtaaagggg
    11521 atatgcttga tatgcagatg gttggtaatg gtcaaacgcg tttgattttc ttgattcctg
    11581 cacgtggttt gattggttat tcaacagagt ttctttcaat gacacgtgga tatggtatca
    11641 tgaatcatac ttttgatcag tatctaccgg ttgttcaagg agaaatcggt ggccgtcatc
    11701 gtggtgcctt ggtttctatt gaaaatggga aagcaacgac atattcaatc atgcgtattg
    11761 aagaacgtgg aactatcttt gtcaatccag gtacagaagt ttacgaaggg atgattgttg
    11821 gtgaaaactc acgtgataac gaccttggtg ttaacattac aacagctaag caaatgacaa
    11881 atgtccgttc agtgactaaa gatcaaactg cagttatcaa gacgcctcgt atcctaacgc
    11941 ttgaagaatc attagagttc ttgaatgacg acgaatacat ggaagtaacg ccagaatcta
    12001 tccgcttgcg taaacaaatc ttgaataaag ctgcgcgtga taaagctaat aaaaagaaaa
    12061 aatcagccga atagcttttg aagttaaact aactaaataa aatagcaaaa agatatttaa
    12121 gaaaagtcat tttttaaagg tctttttgcg tttacaagtt cctatggaat ataaaccaaa
    12181 ataaaggagg acggaatgtt ttaccttatt atggctgtct cgattatatc ttattatcta
    12241 tacatggctc ctaaaagtgt ccgtaacacc cttgggatga ttggtttagt tggcttggtt
    12301 gctctgctta ttgttttagc gggcttgagt tttatcaaaa tcatgcaaac ccctcctgaa
    12361 ttttttattg ggatgggaat ggtggcattg ggctattttg ctttaaaaga tgtgcgtaaa
    12421 atgaccaaaa aaccgagagt taaataaaaa tacttaaata aaaatactta aagattgatg
    12481 attttggttt ttatacgata aaaggactga tttgttttag acaatatcag tcctttttta
    12541 atagttcatt ttttctgaaa gaaagctttt agccgatttt cattgagtgt gcaactaaaa
    12601 agagatgctt ggtagtgaca gttaagacct ctgactagta ttttatctta ttttacttaa
    12661 aaaaacgtat caataatctc acaaatatgg gcatgatgtt tgtatattgg aaacggattc
    12721 taattctaaa ctcaagaacg gtatttttaa aatgttgaaa aagcatctaa ataaaggtaa
    12781 aatagaaatc aaatagaatg gatagtgtga gcttagatga aagtgataag taattttcaa
    12841 aacaaaaaaa tattaatatt ggggttagcc aaatcgggcg aagcagcagc aaaattattg
    12901 accaaacttg gtgctttagt gactgttaat gatagtaaac catttgacca aaatccagcg
    12961 gcacaagcct tgttggaaga ggggattaag gtcatttgtg gtagccaccc agtagaatta
    13021 ttagatgaga actttgagta catggttaaa aaccctggga ttccttatga taatcctatg
    13081 gttaaacgcg cccttgcaaa ggaaattccc atcttgactg aagtagaatt ggcttatttc
    13141 gtatctgaag cgcctattat cgggattaca ggatcaaacg ggaagacaac cacaacgaca
    13201 atgattgccg atgttttgaa tgctggcggg caatctgcac tcttatctgg aaacattggt
    13261 tatcctgctt caaaagttgt tcaaaaagca attgctggtg atactttggt gatggaattg
    13321 tcctcttttc aattagtggg agtgaatgct tttcgccctc atattgctgt catcactaat
    13381 ttaatgccga ctcacctgga ctatcatggc agttttgagg attatgttgc tgctaaatgg
    13441 atgattcaag ctcagatgac agaatcagac taccttattt taaatgctaa tcaagagatt
    13501 tcagcaactc tagctaagac caccaaagca acagtgattc ctttttcaac tcaaaaagtg
    13561 gttgatggag cttatctgaa ggatggaata ctctatttta aagaacaggc gattatagct
    13621 gcaactgact taggtgtccc aggtagccac aacattgaaa atgccctagc aactattgca
    13681 gttgccaagt tatctggtat tgctgatgat attattgccc agtgcctttc acattttgga
    13741 ggcgttaaac atcgtttgca acgggttggt caaatcaaag atattacctt ctacaatgac
    13801 agtaagtcaa ccaatatttt agccactcaa aaagctttat caggttttga taacagtcgc
    13861 ttgattttga ttgctggcgg tctagatcgt ggcaatgaat ttgacgattt ggtgccagac
    13921 cttttaggac ttaagcagat gattattttg ggagaatccg cagagcgtat gaagcgagct
    13981 gctaacaaag cagaggtctc ttatcttgaa gctagaaatg tggcagaagc aacagagctt
    14041 gcttttaagc tggcccaaac aggcgatact atcttgctta gcccagccaa tgctagctgg
    14101 gatatgtatc ctaattttga ggttcgtggg gatgaatttt tggcaacctt tgattgttta
    14161 agaggagatg cctaatgcct aagaagattt tatttacagg tggtggaact gtaggtcatg
    14221 tcaccttgaa cctcattctc ataccaaaat ttatcaagga cggttgggaa gtacattata
    14281 ttggtgataa aaatggcatt gaacatacag aaattgaaaa gtcaggcctt gacgtgacct
    14341 ttcatgctat cgcgacaggc aagcttagac gctatttttc atggcaaaat ctagctgatg
    14401 tttttaaggt tgcacttggc ctcctacagt ctctctttat tgttgccaag cttcgccctc
    14461 aagccctttt ttccaaaggt ggttttgtct cagtaccgcc agttgtggct gctaaattgc
    14521 ttggtaaacc agtctttatt catgaatcag atcggtcaat gggactagca aacaagattg
    14581 cctacaaatt tgcaactacc atgtatacca cttttgagca ggaagaccag ttgtctaaag
    14641 ttaaacacct tggagcggtg acaaaggttt tcaaagatgc caaccaaatg cctgaatcaa
    14701 ctcagttaga ggcggtgaaa gagtatttta gtagagacct aaaaaccctc ttgtttattg
    14761 gtggttcggc aggggcgcat gtgtttaatc agtttattag tgatcatcca gaattgaagc
    14821 aacgttataa tatcatcaat attacaggag accctcacct taatgaattg agttctcatc
    14881 tgtatcgagt agattatgtt accgatctct accaaccttt gatggcgatg gctgaccttg
    14941 tagtgacaag agggggctct aatacacttt ttgagctact ggcaatggct aagctacacc
    15001 tcatcgttcc tcttggtaaa gaagctagcc gtggcgatca gttagaaaat gccacttatt
    15061 ttgagaagag gggctacgct aaacaattac aggaacctga tttaactttg cataattttg
    15121 atcaggcaat ggctgatttg tttgaacatc aggctgatta tgaggctact atgttggcaa
    15181 ctaaggagat tcagtcaccg gacttctttt atgacctttt gagagctgat attagctccg
    15241 cgattaagga gaagtaaatg gcaaaagata aagagaaaca aagtgatgac aagctcgttt
    15301 tgacagagtg gcaaaagcgt aacattgaat ttttaaagaa aaagaagcag caagctgagg
    15361 aagaaaaaaa actcaaagag aaattattga gtgataaaaa agcgcagcag caagctcaaa
    15421 atgcttctga agcggttgag cttaaaactg atgagaaaac tgatagtcag gaaattgagt
    15481 cagaaacgac gtcaaaacct aaaaaaacca aaaaagttag acaacccaag gaaaaaagcg
    15541 cgacacaaat cgcttttcaa aaatccttgc ctgttctttt gggggcgctc ttactcatgg
    15601 cggtgtctat ttttatgatc actccttata gcaaaaagaa agagttttct gtaagaggaa
    15661 accatcaaac gaaccttgac gaattaatca aagctagcaa agtcaaagca tctgactatt
    15721 ggttaacgct gttaacttcg cctggtcagt atgaacgacc gattcttcgt actattccat
    15781 gggtgaaatc tgtacatctc tcttaccaat ttcctaatca ctttctattt aacgttattg
    15841 aatttgaaat catcgcttat gcacaagttg aaaacggttt tcagcctatt ttggagaatg
    15901 gaaaacgtgt ggacaaggtc agggcatcag aactaccgaa atctttcttg attcttaatt
    15961 taaaagatga gaaagcgatc caacagttag ttaagcaatt aacgacatta cctaaaaaat
    16021 tagtcaagaa tatcaagtca gtgtctcttg caaattccaa aacgacagcg gatttactac
    16081 ttattgaaat gcatgacggt aatgtagtta gagtaccgca gtcacaactc acattgaaac
    16141 ttccctatta tcaaaaattg aaaaaaaacc ttgaaaatga tagtatagtg gatatggaag
    16201 tgggaattta tactacaaca caggagattg aaaatcaacc tgaagttcct cttacgcctg
    16261 aacaaaacgc agctgataaa gaaggagata agcctggtga acatcaggaa cagacagaca
    16321 atgattcaga aacgccagca aatcagagta gtcctcagca aacaccacca tccccagaaa
    16381 cggtcctcga acaggcccat ggctagctaa tatctaagtt gaaaaagcaa tgaaaacgtt
    16441 agaaattcaa cgattctaac ccataatgaa ttgcctaaaa aaaattaagt ttatataaca
    16501 aaaaacgtaa aatgataaca ttttacgttt ttttatggta taatattttc tgaatgattc
    16561 tgttttttag cagtttttag aatagcaaaa gtttggaaag taagtgaggt caagtgaatg
    16621 gctagaaatg gcttttttac tggtttggac attggaacaa gctcgataaa agttttagta
    16681 gcagaattta tttctggtga gatgaacgtc attggtgtta gtaatgttcc aagtaccggc
    16741 gtaaaagatg gcataataat cgatatagag gcagctgcga ctgccatcaa aactgcggta
    16801 gaacaagcag aagaaaaagc agggatgaca attgaaaagg ttaatgttgg gctaccggca
    16861 aaccttcttc aaattgaacc aacacaagga atgattcctg tcccaagtga gtctaaagag
    16921 ataaaagatg aggatgttga tagcgttgtt aaatcggctt taacaaaaag tatcacacca
    16981 gaacgagagg ttatctcttt agttccagaa gagttcattg tggatggctt tcagggcatt
    17041 cgagatccac gtggtatgat ggggattaga ttagagatgc gcgggcttat ttatactgga
    17101 ccaagcacca ttttacataa tctgcgtaaa acggtagaaa gagcaggcaa taaagttgaa
    17161 aacatcatta tttctccgtt agctatggct aaaaccattt taaacgaagg tgagcgcgag
    17221 tttggagcta ctgtaattga tatgggaggt ggacagacaa ctgtcgcttc tatgcgagca
    17281 caagaattgc agtataccaa tatatatgcc gaaggcggcg aatacattac taaagatata
    17341 tcaaaagtat taaaaacgtc tttggctatt gcagaagcac ttaagtttaa ttttggtcaa
    17401 gcggagatat cagaagctag tataactgaa acagtaaaag ttgatgtggt aggtagtgaa
    17461 gagcctgttg aggtaactga acgttattta tctgaaatta tttcagcgcg tattcgtcat
    17521 attttagatc gtgtgaagca agatttggaa agaggtcgtt tactagactt accaggaggc
    17581 attgttttga ttggtggcgg tgcaatcatg cctggagtgg tagaaattgc acaagaaatc
    17641 tttggagtaa ctgtaaagct ccatgttcca aaccaagtcg gtattagaaa tccaatgttt
    17701 tcaaacgtta tcagtttggt agaatatgtt ggtatgatgt ctgaagtaga cgttttagca
    17761 caaactgcag tttcaggaga agaacttttg cgacgcaagc ctatcgattt cagtggccaa
    17821 gaatcttatt taccagatta tgatgattca agaagaccag aatcgaccat tggctatgaa
    17881 caacaagcgt cacaaacagc atatgattca caagttccga gtgatcctaa acaaaaaatt
    17941 tcagaacgtg ttcgtggcat atttgggagt atgtttgatt aaaagtaata aagtgaggaa
    18001 ataaaatggc attttcattt gatactgcat caattcaagg tgcaattata aaagtaattg
    18061 gagtcggcgg aggtggcgga aatgccatta atcgtatgat tgatgaaggt gttgctggtg
    18121 tcgagttcat cgcagcaaac acagacattc aggcattaag ctcatcaaaa gctgaaacgg
    18181 ttattcaact aggccctaaa ttaactcgtg gacttggtgc tggaggacaa cctgaagtag
    18241 gacgtaaagc tgctgaagaa agcgaagaaa ttttaacaga agctcttaca ggagcggaca
    18301 tggtatttat tactgccggt atgggtggtg gctctgggac aggggctgca ccggttattg
    18361 ctcgtatcgc taaaagtttg ggagccttga cagtagctgt tgttactcgc ccgtttggtt
    18421 ttgaaggtaa caaacgtggt aattttgcta ttgaaggtat cgaagaacta cgtgaacaag
    18481 ttgatacttt gttaattatt tcaaataata accttcttga gattgttgat aaaaagacac
    18541 ctttattaga agcacttagt gaagctgata atgttttacg ccagggagtt caagggataa
    18601 ccgacttaat tactagtcct ggccttatca atctcgattt tgccgacgtg aaaacagtta
    18661 tggcaaataa agggaatgcc ttgatgggga ttgggattgg ctctggagaa gagcgcattg
    18721 ttgaggcggc gcgtaaggca atctattcac ccctattaga aacgactatt gatggtgcac
    18781 aagacgttat tgtgaacgtt acaggaggtc tcgacatgac acttacagaa gctgaagaag
    18841 cctctgaaat tgttgggcaa gctgctggtc aaggcgttaa catttggtta ggaacatcta
    18901 ttgatgatac tatgaaagat gacatccgtg tgactgttgt agcaactgga gtgcgccaag
    18961 aaaaagccga acaagtttca ggttttcgtc agcctaggac ttttacccaa accaacgcgc
    19021 agcaagtagc gggtgcacaa tatgcatcag atcaagcaaa acagtcggtt caaccagggt
    19081 ttgatcgtcg ctcaaatttt gattttgaca tgggggagtc tcgcgagata ccaagtgcac
    19141 aaaaggtaat ttctaatcat aatcaaaatc aaggttctgc ttttggaaat tgggatttga
    19201 gacgtgataa tatttctcgt ccaacagaag gtgaattgga taaccatctt aatatgtcaa
    19261 cgttctcagc taacgatgac agtgatgatg aattagaaac gcctccattc tttaaaaacc
    19321 gttaataatg gatttactga caaataaaaa gaaaattttt gagactatcc gcttatctac
    19381 agaggcagca aataggacta atgatagtgt ttcagttatt gctgtaacaa aatatgtgga
    19441 tagtacaatt gcaggtcagc ttatcgaagc aggaattgag cacattgccg aaaaccgtgt
    19501 tgataaattt cttgaaaagt atgatgcgtt aaagtatatg ccagtaaagt ggcatttaat
    19561 tggtacctta caacgtcgta aagtcaagga agttatcaat tatgttgatt attttcacgc
    19621 tctagattct gtgagattag ctttggaaat caacaagaga gctgaccatc ctgtgaagtg
    19681 ttttctacaa gttaatattt ctaaagaaga gagtaaacat ggttttaaca tttctgagat
    19741 tgatgaagcg attggagaaa taggtaagat ggagaagata cagttagttg gtttaatgac
    19801 tatggcacca gcaaatgcca gtaaagaaag tattataact atttttcgac aagcaaatca
    19861 attaagaaaa aacttgcagt taaaaaaaag aaagaatatg ccttttacag aattgagcat
    19921 gggcatgagt aacgattatc caattgctat tcaagaaggc tcaactttta ttcggattgg
    19981 tagagctttc tttcactaat ggagaataag atggctttta aagatacatt taacaagatg
    20041 atttcttatt ttgacacgga tgaggttaac gaagttgaag aagatgttgc agcatcaact
    20101 gataacgtta ttccaagatc acaacaatca gtcagagcaa gtagtcatcc aaaacaagaa
    20161 cctagaaaca atcacgtaca acaagatcat caagcgagat cccaagaaca gacaaggtca
    20221 caaatgcatc caaaacatgg cacttctgaa cgctattatc agcagtctca gccaaaagaa
    20281 ggccatgaaa tggttgacag aagaaaacgg atgagcactt ctagtattgc aaatcgccgt
    20341 gagcagtatc aacaatcaac ttgttcagat caaacaacta ttgccttaaa gtatcctcgt
    20401 aaatatgagg atgctcaaga aattgtggat cttttaatag ttaatgaatg cgttttgatt
    20461 gattttcagt ttatgctaga tgctcaggct agacggtgtt tagattttat tgatggtgct
    20521 agtaaagtgc tctatggtag cttacaaaag gtcggctctt caatgtactt actggctccg
    20581 tcaaatgtat ccgtcaatat agaagaaatg actatcccac atactacaca agatattggc
    20641 tttgattttg atatgaaaag gcggtaaata aatgatatta atactatcta ttcttcttcg
    20701 tctgatcaaa gtttacactt atttattgat tgcttacgca ttaatgtcat ggtttcctgg
    20761 ggcatatgat tcaaaaattg ggcgtttgat tagtggtatc gttgaaccaa ttttaaaacc
    20821 ttttagagca tttaatttac aatttgccgg tcttgacttc actatttttg tcgtcattat
    20881 tagtttgaat tttttagctc aagttttggt ccgtgtgttt atttaatggt tagtcatagt
    20941 aagatttatc agcattttca ccaagaagaa tatcctttta ttgatagaat gtctgatatg
    21001 attaatagag ttgaagatta ctatctttta gaagttactg agtttttaaa tcctagagaa
    21061 gtgatgattt taaaaagttt gattgcttta acagatctaa aaatgttcgt atcaacagat
    21121 tactacccaa gcgaatatgg tcgtgtcatt attgcacctg gttactatga cttagaacaa
    21181 agtgattttc aaatagcttt agtagagata agttatcagg caaagtttaa tcagttgaca
    21241 catagtcaaa ttttaggaac tttaattaat gaattaggag taaagcgaaa tttatttgga
    21301 gatgtttttg ttgaaatggg atatgcccag ctcatgatta agcgggagtt attggattat
    21361 tttttaggaa caattactaa aatagctaaa actagtgtga aattaagaga agttaacttt
    21421 gatcagttaa ttaggtctat tgataacagc cagaccctgg atattctagt ttctagtttt
    21481 cgattagatg gtgtagttgc tactatctta aaaaaatctc gaacgcaagt tatagcatta
    21541 attgaagcaa ataagattaa ggtaaactat cgagttgcta ataaagcttc agataatcta
    21601 gtcatagggg atatggtgag tatcagaggt cacgggcgtt ttactctttt agcagataat
    21661 ggagtgacca aacatggcaa acaaaaaata acactaagta aaatgataca taaataagga
    21721 gatacaatgg cacttacaac gctagaaatt aaagataaaa cttttaagac aaaattccga
    21781 ggatactgtg aagaagaagt caatgaattc cttgatattg ttgttgatga ttacgaagct
    21841 cttgtacgta aaaatcgtga taacgaagca agaattaaag atcttgaaga aaaattatct
    21901 tactttgatg aaatgaaaga gtcacttagc cagtctgtaa tcttagctca agaaacagct
    21961 gaaaaagtga aagcaacggc taatgcggaa gcaacaaact tggttagtaa agcgacttat
    22021 gatgctcagc atttattaga tgaatctaaa gctaaagcca atcaaatgtt gcgtgatgca
    22081 actgatgagg ctaagcgagt tgcgattgaa acagaagaac taaagcgtca aacacgtgtg
    22141 ttccaccaac gtttaatctc atctattgag tcacaattaa gcttatcaaa ttcacctgaa
    22201 tgggatgaac tgctacaacc gactgcaatc tatcttcaaa attctgacga tgcttttaag
    22261 gaagtcgtga agaccgttct taatgaagac attcctgaat ctgatgatag tgcctctttt
    22321 gatgcaacac gtcagtttac tccagaagag ttagaagaat tgcaacgtcg tgttgatgaa
    22381 agcaataagg agttagaggc ttaccaactt gactctcaat ctgattctac gactgagcca
    22441 gaggtaaatc tcagtgaaac acaaacgttt aaattaaata tctaaataaa gaaaaacaag
    22501 atgatttgct tataatcaca gcaagcggga gatgatgaga gtctcgtatt ccctaagtat
    22561 tatcattatc attttctaaa attttcgctg aactacagta agcgaggagg tttgttcacc
    22621 ttacgagcaa agagggagcg gtaagtctta tgatatatta tcgctctgaa ctaaggtggt
    22681 accacgagct ttcgtccttt ttgagacgag gcttttttcg ttatagtcgc aagtttgttt
    22741 aaaaggagtt tgaaataatg aaattaaaag agacacttaa tctaggaaaa acagcctttc
    22801 caatgcgcgc aggtcttcct aataaagagc cacaatggca agcagcttgg gaacaagcag
    22861 agctttacaa aaaacgtcaa gaattgaatg caggtaagcc tgcctttcat cttcatgatg
    22921 gacctccata cgctaacggc aatattcacg ttgggcatgc ccttaataaa atttcaaaag
    22981 atatcattgt gcgttctaag tccatgtctg gctttcaagc gccttatgta cctggttggg
    23041 acacacacgg ccttccaatc gaacaagtct tggctaagca aggaatcaaa cgtaaggaga
    23101 tggatttagc agaatacctt gagatgtgtc gtcaatacgc tttaagtcag gttgataaac
    23161 agcgagatga tttcaaacgg ttaggtgttt cggcagactg ggaaaatcct tatgtcactt
    23221 tggacccaca gtttgaagct gatcaaattc gtgtttttgg agcgatggct gaaaaaggtt
    23281 acatctatcg tggtgctaaa cctgtttatt ggtcatggtc ttcagaatcg gccttggcag
    23341 aggctgaaat tgaataccat gacatcgatt caacctcttt atactacgcc aataaagtta
    23401 aagatggtaa aggtatcctt gatactaata cgtatatcgt tgtttggacc acaacaccat
    23461 ttaccgttac agcatcacgc ggtttgactg ttggtcctga tatggattat cttgtggtga
    23521 aacctgctgg ttcagaccgt caatatgttg tggcagaagg tcttttggat agccttgctg
    23581 gaaaatttgg ctgggaatct tttgaaactt tagctagcca taaaggagct gatttagagt
    23641 acattgtgac agaacaccca tgggatactg acgtagaaga attggttatc cttggtgacc
    23701 atgttaccct tgagtcaggg acagggattg tccacacagc accaggtttt ggtgaggatg
    23761 actacaatgt tgggacaaaa tacaaactgg aagttgctgt gactgttgat gaacgtggcc
    23821 taatgatgga aaatgcaggt ccagatttcc acggacaatt ttataacaag gtaacgccga
    23881 ttgtcattga taaacttggt gatcttttat tggcacaaga agtgatcaat cactcttacc
    23941 catttgactg gcggacgaaa aaaccaatca tctggcgtgc tgtaccgcaa tggtttgctt
    24001 ctgtttctga tttccgtcaa gatattttgg atgaaattga aaaaacaacc tttcatccat
    24061 catggggtga aacacgcctt tacaatatga ttcgtgatcg tggcgactgg gttatttctc
    24121 gtcaacgtgc ttggggagtt ccgcttccta tcttctatgc cgaagatggc acagctatta
    24181 tgactaaaga agtgacagat catgtggctg atttgttcca agaaaatgga tcaatcattt
    24241 ggtggcaaaa agaagctaaa gacctattac cagagggctt cactcatcca gggtcaccaa
    24301 atggcgagtt tacaaaagaa actgatatca tggacgtttg gtttgactct ggttcttctt
    24361 ggaatggagt catgaatact aaagaaaatc tttcctatcc agcggatcta tacttagaag
    24421 gctctgacca gtatcgtggt tggttcaatt catccttaat tacatctgtg gctgttaatg
    24481 ggcatgctcc atataaggct attttatcgc aaggctttgt tcttgatggt aagggcgaaa
    24541 aaatgtctaa atcaaaaggc aatatcattt ctcctaatga tgtggctaaa caatatggtg
    24601 cagatattct tcgactttgg gtagcttctg tggataccga taatgatgtc cgtgtttcta
    24661 tggagatcct tggtcaagtc tctgaaactt accgtaaaat ccgtaacacc cttcgcttct
    24721 tgattgctaa tacatctgat ttcaaccctg ctacggatac agtagcttat gcagatttag
    24781 gaactgttga taagtacatg acaattgtct ttaatcagtt ggtagcaacg attactgacg
    24841 cttatgagcg ttatgacttt atggctattt acaaagcagt ggtgaacttt gttacggttg
    24901 atttatcagc cttctatctt gattttgcta aagacgttgt ctatattgaa gctgccaata
    24961 gcttagaacg tcgtcgcatg cagacagtct tctatgatat cttggttaag attaccaagt
    25021 tattgacgcc aatcttgcct cacactactg aagaaatctg gtcttatcta gagtatgagt
    25081 cagaagcatt tgttcaattg gcagaaatgc ctgtggcaga aaccttctcg gctcaagagg
    25141 atattttaga agcttggtca gctttcatga ctttgcgtac tcaagctcaa aaagctttgg
    25201 aagaagcgcg aaatgctaag attatcggta aatcattgga agcccatttg accatctacg
    25261 ctagtgaaga agtgaaaacc ttattgactg ctttagacag cgatattgct ttgcttttga
    25321 tcgtgtctca attaaccatt gctgacttgg cagatgcgcc tgcggatgca gtggcatttg
    25381 aaggtgttgc ctttatagta gaacatgcca taggtgaagt ttgtgagcgt tcacgtcgca
    25441 tcgacccaac tactcgcatg cgttcttaca atgcatttgt ctgtgatcac agcgctaaaa
    25501 tcattgaaga aaatttccca gaagctgtgg ctgaagggtt tgaagagagt ggcaaataag
    25561 tcttatcaag acttgaagtg acactgagta agaagacaac catcgggtct taggacctga
    25621 tggtttttta gtagagctga ttacgtccat tagccagctt taacgagaga caaagtagga
    25681 gaaatttaga ctactttttt aatttctttt aagaaaattg ctggcttcat ataaggacta
    25741 ttaagataga gttcaccttt cgctaataaa aaaacttagc tcttttagaa gggctaagtt
    25801 ttgtctgaat agctggctgc tgtttataca gaagtttgaa atgaaatttc aataaggtta
    25861 ttcgtgtgaa gtgtttttga ttgtccagga ataacaattt taggatcaat tttacgtttt
    25921 aaaaaaaatt cgcgaatgat atcaagttca tcctccttga tggcacggta tttatttgaa
    25981 ataagtagtt cgtaatgttt tggtgcttgg gtaaaaatga catctgtatt tcctgctgag
    26041 tatacttcga ctaaataggc atcggtgttt tttagctgat ttttgatgag ttctgggtga
    26101 ctgctagtgg tattgatgag tctcataaat gtcctcctta gtattcctgt acctattata
    26161 gcactaattt aaagccttag ttagtagaat gatgcttttt ttgccattcc ttgactcccc
    26221 attgatggct ctctcgtttt aatttagcaa ttgcctcgat aggagaaaac caacctaagt
    26281 tattaaaatc ttctaagggc ttagaaacgg cttgaaatgc agttacttca taaagatagg
    26341 caggatggta gaagtgggta tcacgatggc gagagtagaa atattcatct gcctgaccgt
    26401 aatatgaacc aatagtagca gaaaaaccaa gttcttcaat cagttcacgc tctagggcct
    26461 gaagctgacc ttcacctgct tcaatttcac caccaggtaa aaaccaggaa ccattaggag
    26521 cttgaacaag aataattttt gtttgttcgt gattgggaat gatagcatag acaccgtaac
    26581 gtgtgacata gtctttatgt gcatttttat gtccaaatgt tggaatcatc attttattta
    26641 cctttccttt ttcttcatta tactaaattt ttgctaatta ttcttatcaa tttgtttagt
    26701 ttaataaaaa attgatagaa aaagagagag tctccttata gaaactctct tagctaacgt
    26761 taatcgagag actcttcgtt aagaaaagaa tctccctctt taacagcttt aatcgtgata
    26821 ttttcactgt ttgtcatcag tgcacgtaac tgtttttcag ttgggtgttc taaataatag
    26881 tctgtaatgg catcttcgat ataatcttga atagtacgac ggagcggccg agcgcccatt
    26941 ttaggatcat agcctaaatc tactaatttt tctttgacct tttgtgtcac atcaagatga
    27001 atcccattat agcctaagcg ctcattaaca tcttccagca ttaaatcaac gatatgcagc
    27061 aaatgctctt tagataaagc tttgaattca ataataccat cgaaacgatt cataaactct
    27121 gggctgaaga agttgcttaa ttcaccaagg acagaacttg tccgtccttc tctagcagca
    27181 ccaaatccga cagaagcttc gcttttacct gttccggcat tagaggtcat gataataatg
    27241 gtgtccttga agctgactgt tcgtccttga ccatctgtta aacggccatc atcaagaact
    27301 tgtaagaaca tgtgcatgac gtcaggatgg gctttttcca cctcatctaa gagaataagt
    27361 gaatatggat tgcgacgaac ttgttcggtt agctgtccag cttcttcata gccgatataa
    27421 cctggaggag ccccgactaa tttggcgaca gcgtgttttt ccatgtattc ggacatgtca
    27481 aagcgaatca tattgtttgt cgaaccaaag agttcaatgg ctagttgttt agacaattca
    27541 gttttaccaa ccccagtcgg tccaacgaat aagaaagaac caatagggcg atttggagtt
    27601 cctaatccca cacggttacg acgaatagcc ttagcgattt tatcaacagc atcgtcttgc
    27661 ccgatcacgt gtgctttcag atcattggct aagtttacga gttgagactg ttccttttct
    27721 tttaagtccc caactggaat attagttttt tgctctacaa tagcttcaat ggttttttca
    27781 gtaatgatgg gaatatcttg ctcatcgact ttttgagcct gcatttcttt gtattttgta
    27841 atttgatcgc ggaaataagc tgcgcgttca tagtcttcgt ctcgagtagc ttgcgccttg
    27901 agattctcag cttcaataag acgtttgtca atttctttag gatcaacaaa gttgagggtt
    27961 agattcattt tggaaccagc ttcgtccaga aggtcaatag ccttatcagg aagaaaacgg
    28021 tcttgaatat agcggttaga taaatgagcg gcagcttcaa tagcggctgg gctatatttg
    28081 acatgatgat agtcttcgta tttcggttgg atacctttta aaatcgtgat ggtttcctca
    28141 acagaaggtt catcaacttt aacgggttgc atacgtcgtt ctaaggcagc gtctttttca
    28201 ataatacggt attcatttaa tgtagtagca ccaacgagtt gcaactcacc acgggccaag
    28261 gctggtttta aaatattacc agcatccata ttgccgtctc ctgcagaacc agcaccgaca
    28321 atctcatgaa tttcatcaat aaagagaatc acatccttgc gattgcgaat ttcttccatt
    28381 aatttttgca tgcgctcttc aaactgacca cggatacctg ttccctgaac gaggctaacc
    28441 acatcaagac gaatcacttg cttgccttgg agtttttgag gaacagtacc atcaataatt
    28501 ttttgagcca aaccttctac aacagcagtt ttaccaactc caggctcacc aattagcaca
    28561 ggattatttt tagtacggcg gttgaggatc tcgataacgc gtgtaatctc ttcgtcacga
    28621 ccaataacag gatcaatatt accatttctt gcaatatctg tgacattaat cccaaactct
    28681 tctaacaagc cctttgcttg ctgatttggt gtttgtggct gagcaggtcg ttgtccgttg
    28741 tagttaccac catagcgtcc tccgccattt ccattttgcc ctgcctgagt aggtggggta
    28801 tttggaagat taccaaaagc tctaaaatta ttcaaatcac caaaaaagtc atcaaagaaa
    28861 ggacttgtgg atctatcttg tgctctatat cctggggtta ggccatttaa aatagaattg
    28921 gcaggatcgg atttcatgat ttgataacaa ttttgacaga gatcaacctg tctttgtttt
    28981 ccattaacac ttgtataaag atgaatagta gattcgttta aattacaatt ttgacacagc
    29041 ataatatacc tccgagggtt tatctaaaag tcaaagtcat ttagtcaaaa atggtcaaac
    29101 tttatattca tattatagca ccacggcttg ggaaatcaag taaaaagttt atcgctttag
    29161 cgttagcttt tagagagtaa catgacagag taactttatt aagtttccaa tgagcaaatg
    29221 tttaaaatca ttgaaaagag taaaaaaagt aaaatatcaa taaatatatt actttccttg
    29281 ttagtttccc ttgttttgtg ttaaaataag agggataaaa aacgaatagg agaaacgaaa
    29341 tggaatcaca tttggtgaga atcatcaatc gtttggagtt gatggtgaca gatggcggta
    29401 atctcaagcg aaattttgaa cgcgatggtg ttgttgttgc agaagttgcc ttttcaaatg
    29461 atcctgaaaa tggacctgtt ttcacattgc gtgatgttga agcgcgtgag tcatattcct
    29521 ttgatagcat tgacttgatt gcaatggaaa tctacgattt attatattaa gcttgacata
    29581 gtaaagagac ttgcctgagg caggtttttt tattgtcaaa aagtattgta ttaatattca
    29641 tcagatggta taattatctt tattgtaatg aacctaggac ttatgaggag atataaatgg
    29701 atttgtcatt tttgcccaaa tactgggcct actttaacta cggtgtactt gtcaccatta
    29761 tgatttcagt cagcgttgtc ttttttggaa cccttattgg tgtcttggta accctgatta
    29821 agcgtagtca tgtgaagccg ttgacctggg tcgttaatct ttacgtttgg atctttcggg
    29881 gaacacctat ggtggttcaa atcatgattg cctttgcttg gatgcatttt aacaatatgc
    29941 ctactattgg ttttggggtt ttagacttgg acttttcaag actacttcct ggaattatta
    30001 tcatttcatt gaatagcggt gcttatattt cagaaattgt tagagcaggt attgaggctg
    30061 taccaaaagg gcaattagaa gcagcttatt cactaggtat tcgtcctcaa aatgccatgc
    30121 gttatgtgat tttgcctcag gcctttaaaa atattttgcc agccttagga aatgaattta
    30181 ttaccattat taaggatagt gctcttttac aaaccattgg agtgatggaa ctttggaatg
    30241 gtgcccaatc ggtggtaacg gctacttatt ctccaatttc ccctttactg gtggctgctt
    30301 tttactactt aatggtcaca acagtgatgg cacagttatt ggcagtctta gaacgtcaca
    30361 tggcgcaagg aggtaatcat tgatgactga ggctattatt tcaattaaag acttgcacaa
    30421 gtattatggg cacaatgaag tgcttaaggg aattgattta gacattatgc caggagaggt
    30481 tgtggtgatt attggccctt ctggttctgg aaaatcgacc ttacttcgaa ccatgaatct
    30541 tttggaagta ccaactaagg gacagattag gtttgagggg attgatatta ccgataagaa
    30601 gaatgatatt ttcagcatgc gtgaaaaaat gggaatggtt ttccagcagt ttaacctctt
    30661 tcccaatatg actattttag aaaatatcac tttatcgcca atcaaaacta agggaatggc
    30721 taaagcagag gctgacaaaa cagccttgag cttgttggac aaagttggat tatcagaaaa
    30781 agccaaggct tatcctgcta gtctttctgg tgggcaacag cagcggattg cgattgcgcg
    30841 tggactggct atggatccag atgttttact ctttgatgaa ccaacttcag ctctagaccc
    30901 agaaatggtg ggtgaggtct tggctgtcat gcaagatttg gctaaatctg ggatgactat
    30961 ggttattgtg actcatgaaa tgggctttgc taaagaagtc gcagaccgtg tgatgtttat
    31021 ggatggcggc gttattgtgg aagaaggaag tcctaaccaa ctttttgatc tgactaaaga
    31081 agaacgtaca aaagattttt tgagtagagt attataaatc tgttctatga attataaaag
    31141 gaagtcctaa atgatttagg acttcctttt ataatttgct aaggcatttt ataggagatt
    31201 acttttcttt ttgttaaaat aaaaagaaat agttgcatta tttgcataga agaaagggat
    31261 ctgttatggt ttcagtgatg tggtttcgta gagatttaag gattgatgat caaaaagctt
    31321 tagctcatgc tattgctagt caatcaccaa ccttatgtgt gtttcaattt aacaaggagc
    31381 aattaaatct gctacatagt cggaatcaat ctagttttat tactagcgtt ttggctttta
    31441 gaaagttatt aaaatgtgaa ggtattgact tatatctaat gtatggagac ttaatgacct
    31501 gttttgagca attattgaca caattaaaag actggacaga tgtgtatttt aactatgatg
    31561 aatctggata tggtcgttta cgtgatcaaa aagcagcaca atttttcaaa aaaaatggga
    31621 ttgctgttca tacctaccaa gatcactatt tacatggtag tcaagagatc attaaccaat
    31681 caggtcagcc ttataaagtt tttacgccct actataggat ttggcaaaat tatccgaaag
    31741 agactcctat taaagtagaa ctatcgcaag gcaggtggtt aaatcttgag acaccagatg
    31801 atgttttaag gactgttgag agttttaaag atgaaaaata tcaggatgtt gcaacttttg
    31861 atgaagcgtc aaaacaacta aatcgattta ttcaagacca attagcagct tatcacgcta
    31921 atcgagattt tccagcgcag ctagggacga gtcggttgtc tcctttttta agaataggag
    31981 ctattggcat tcgcacggta tatcacgctg tgcgtcaagc accaaatagt ctagggcaag
    32041 ctactttttt aaaagaatta gcttggagag acttttacaa tatggtctat gtggcttatc
    32101 ctgatcagaa aactcagcct atccaaaaag ccttttctca aatcgaatgg gttaataatc
    32161 ctgactggtt tcagttgtgg aaagaaggta aaacgggtta ccctattgtc gatgctgcaa
    32221 tgcttcagtt acaaaagaca ggatggatgc ataatcgttt acgaatgata gtagcttctt
    32281 ttttaactaa agatctttta tgtgattggc gtttaggaga gcagtatttt caacaacaac
    32341 taattgatta tgatgctgcc agtaatatcg gaggatggca gtgggctgct tccactggaa
    32401 cagatgctgt tccttatttt agaatattta atcctgttac tcaaggtaag cgctttgatc
    32461 ctaaaggcga gtttattaaa gcgtatttgc ctcaactaga acacgttcca gagaagtatc
    32521 tccacgaacc gtggaagatg ccaaaaaacc ttcaagagag tgtgtcctgt attattggca
    32581 cagactatcc tcaaccaatt gtcgatcatg ctaaacaacg tgaacaagct attgcaaagt
    32641 atgagtgggc aaaagaaaaa gcaaaaatag aatgactgtt ctcattataa gagtttaaac
    32701 aaaaagttat catgaattaa gattatttaa tttaaaaaat aagttgactt tatttagatt
    32761 atttgatact attcagttgt ctcccttaga gaaagcgatt tcttgttttg gagctgattt
    32821 ttaccatttg gtcttagaat aggatgagta atatgactta caacgaggta tatcaagaat
    32881 ggttgcacaa taatgatctt agtgatgata ttaaagcaga tttagcagcc ataaaagaca
    32941 atgaggctga gattcaagat cgtttttaca aaacacttga atttggaaca gcagggctaa
    33001 gaggaaaact tggagcaggc accaatcgca tgaataccta tatggttggt aaggcagcac
    33061 aagctttagc taatactatt attgatcatg gacctgaagc ggttaaaaag ggcattgcgg
    33121 ttagttatga tgttcgctat caatctagaa catttgcaga attaacatgc tctattatgg
    33181 cagctaacgg tattaaggcg tacctttata aagggattcg gccaacacca atgtgttcgt
    33241 acgctattcg tgctttggga tgtatttctg gtgttatgat tacggctagt cacaatcccc
    33301 aagcttacaa tggttacaaa gcttattggc aagaaggttc tcaaattttg gatgacattg
    33361 cggatcaaat tgcacaacac atggctgcac taactcagta tcaggaaatc aaacaaatgc
    33421 cttttgaaaa ggctctggac agtggacttg ttacttatat tgatgagagt attgaagaag
    33481 catataaaaa agaagtgctt ggtttaacga ttaatgatac tgacattgat aagtcagtcc
    33541 gagtagtcta taccccttta aatggtgttg ggaatctacc agtgcgtgag gttttaagac
    33601 gccgcggttt tgaaaatgtt tatgtggtac ctgagcagga aatgcccgat cctgatttta
    33661 caacagttgg ctatcctaat cccgaagtcc ctaaaacttt tgcatattca gaaaaattag
    33721 gtaaggcagt tgatgctgat attttgattg caactgatcc agattgtgac cgtgtggctt
    33781 tagaagttaa aaatgcagtc ggtgactatg tttttctcaa tggcaataaa atcggagcgc
    33841 ttttatctta ctatatcttt tcacaacgat ttgacttagg caatttaccg gctaatcctg
    33901 ttttagtgaa atccattgta acaggagact tgtcacgggc tattgctagc cattatggta
    33961 ttgaaaccgt tgaaacattg actggtttta agaatatttg tggcaaggca aacgaatatg
    34021 atgtgacgaa acaaaaaaac tacctgtttg gttatgaaga aagtattggt ttttgctatg
    34081 gcacttttgt tcgtgacaaa gacgctgtta gcgcctctat gatgattgta gaaatggcag
    34141 cttactacaa gaaaaaaggg cagaatttac tagatgtttt gcagactatt tatgcgacat
    34201 ttgggtatta taatgaacgt caaattgcct tggagcttga ggggatagaa ggacaaaaac
    34261 gcattgcgag gataatggaa gatttcagac agacaccaat agctagtgtt gcagagatgg
    34321 cgttagacaa gacaattgat tttattgatg gctatcaaga ttttccaaag caaaactgtc
    34381 tcaaatttta tttggatgat ggttcttggt atgcacttag accatcagga acagagccta
    34441 aaataaaatt ttatttatat actattggcc aaacacaaga aaatagtgca acaaaattag
    34501 atgctattga agcagcgtgt cgcacaaaaa taaatcaggt taattaaaca tcaaaaatgg
    34561 ataacacttt tacactattt ttccatataa acaactacaa aaggaggtga gacttttagg
    34621 caccttcttt ttcttgtagg tgccactaat caatttaatc gactcaaacc aatactaaaa
    34681 aataagtaag agtataaaga acaggcatct atttttttgt tataatagaa aaatgaaatt
    34741 aaagggagat gtcaatgaca gaactaattg atggtaaagc cttagctcaa aagatgcaac
    34801 aagagttagc agctaaagtc aacaatctaa aacaaaaaaa aggaattgta ccaggcttag
    34861 ccgttattct tgtaggtgat gatcctgcta gtcaggtgta tgtccgtaat aaagagcgtg
    34921 cagctcttac tgtaggtttt aaaagtgaga ccgttagatt atcagaattc atttgtcaag
    34981 aagagcttat tgcagtaatc gaacgttaca atgcagataa cactattcat ggtattttag
    35041 tgcagctacc cctgccaaat catattaatg ataaaaaaat tattctcgcc attgatccca
    35101 aaaaagatgt ggatggtttt cacccgatga atacaggtca cctttggtca ggacgtccct
    35161 tgatggttcc ttgtactcca tcagggatta tggaattgct tcgagaatat aatgttaacc
    35221 ttgaaggtaa acatgccgtc attattggca gatcgaatat cgttggaaaa ccaatggcac
    35281 agctcttact ggacaaaaat gcaacagtca cgttgacaca ttcaagaaca cgtcaattag
    35341 aagaagtatg tcgctgtgca gatgtgttga ttgtggcaat cggacaaggt catttcataa
    35401 caaaacaata tataaaagat ggtgcgatag tgattgatgt aggaatgaac cgtgatgata
    35461 atggcaagct aattggagat gtggcctttg atgaggtggc agaagttgca gcgaaaatca
    35521 cccctgtacc aggaggtgtc ggtcctatga cgattgctat gttgctagag caaacttatc
    35581 aatctgctct ccgtagtact cataaatagt tataattaga caattcaaca taagagaata
    35641 taagaagtca gcaatggctt ccttttttgt ttttctgctc actatttatt cagaaaaatg
    35701 gtataattgc attagtatgc aaagggacta tgtcaggtcc aactaagtga aaggtggctt
    35761 gatggcagat tatttaaccg tcactcattt gacgaaatat ttaaaattaa aatttgaccg
    35821 tgacccttat ttggaacggg tttatctgac tggtcaagtg tccaattttc gaaaacgacc
    35881 aactcatcaa tatttttcct taaaagatga aagtgctgtg attcaagcca ccatgtgggc
    35941 aggagtctat aaaaaactag gattcgacct agaagaaggc atgaaaatta atgtaattgg
    36001 gcgagtccaa ctttatgaac ctagtggctc ttactctatc gtgattgaaa aggcagagcc
    36061 agatggtata ggtgccttgg ctttgcagtt tgaacaatta aagaaaaaat taacggcaga
    36121 aggttatttt gagcaaaaac acaagcagcc cttgccacag tttgtatcaa aaattggggt
    36181 cattacaagt cctagtggtg ctgtgattcg agatattatc acaaccgttt cacggcgttt
    36241 tccaggggtt gagattttat tattcccaac taaagtacaa ggtgatggag ccgcccaaga
    36301 agtagtggct aatattcgaa gagccaatca aagagaggat ttggacttgc tcattgtcgg
    36361 ccgtggaggt ggctcgatag aagacctttg ggccttcaac gaggaaatag tggttcaggc
    36421 tatctttgaa tcgcaacttc cagtgatttc gagtgtgggt catgaaacag acacgacttt
    36481 agcggatttt gtggcagatc gcagagcagc cacaccaaca gcagcagcag agttagcaac
    36541 gcctattaca aaaacagatc ttatgtcttg gatagtagag cggcaaaatc gttcctatca
    36601 ggcctgcttg aggcggatta agcaacggca agagtgggtg gataaactct cgcaatctgt
    36661 tatttttagg caaccagaac gattatatga tgcctacctg caaaaaattg atcgccttag
    36721 catgaccctg atgaatacga tgaaagatcg tctgagttcc gccaaagaaa acaaggttca
    36781 gttggaccat gccttggcca atagtcaatt acagacaaaa atcgagcgat accaagaccg
    36841 tgttgctacc gctaagcgtt tactgatggc taatatggct agtcaatatg atagtcagct
    36901 ggctcgcttt gaaaaagcac aggatgcttt gctatcactg gatgccagcc gaattatcgc
    36961 ccgtggttat gcaatgattg aaaaaaatca agcactggta gcctctgtaa gtcagataac
    37021 aaagggtgat cagttaacca ttaagatgcg tgatggacaa ttagatgtag aggtaaaaga
    37081 tgtcaaaaac gaaaacattt gaagaaaatt tacaagattt agagactatt gtgaacaaac
    37141 ttgaaaatgg ggatgtccct ttggaagaag ctatttcaga atttcaaaaa ggaatgcttc
    37201 tctcaaaaga acttcaaaag accttacaag cggcagaaaa aacactcgtc aaagtgatgc
    37261 aagctgatgg cacagaagta gatatggatg attaatggac aaattagcta gaattgacga
    37321 agccattcgt cgctactata agacgactag taacggtgta tctgaagagt tgattgatgc
    37381 tatcttgtat tctgttgaca gtggtggcaa gcgcattcgc ccacttatct tattagagat
    37441 gattgaggga tttggggtat cgttacagaa tgctcatttt gatttggctg cagctcttga
    37501 aatgattcat acaggaagtc tgattcacga tgatttgcca gccatggata atgatgatta
    37561 ccgacgtggc agactgacca atcacaaaca atttggagaa gccacggcca ttttggcggg
    37621 agacagctta tttttagacc cctttgggtt aatagctcaa gctgaattaa atagtgaggt
    37681 taaagtagcc ttaatccaag aactttccct agcttcagga acttttggca tggttggtgg
    37741 tcagatgtta gatatgaaag gggaaaatca agcattaagt cttcctcagt tgtcactgat
    37801 tcacctcaat aaaaccggaa aattattagc ttttcctttt aaagcagcag ctcttataac
    37861 agaacaggct atgactgttc gtcaacaact agagcaagcg ggaatgctca tcgggcacgc
    37921 ctttcagatt agggatgata ttttagacgt gacagctagc tttgaagatc ttggaaaaac
    37981 gcctaaaaag gacttattcg cagaaaaagc tacttatcca agtttactgg ggttagaagc
    38041 ctcttaccaa ctgctgacag agagtttaga tcaggctttg acgatttttc agacactaga
    38101 aagcgatgta ggctttaagc ctcaaataat tacaaaactg atagaagggt tacgacttaa
    38161 tgcctaaaga aagagtagat gtgttagcct ataagcaagg attatttgag accagagagc
    38221 aagctaagcg tggtgtcatg gcaggcttag tggtctctgt gattaatggc caacgctatg
    38281 acaagccagg tgacaaaatt gacgatggga ctgagttaaa acttaaaggt gaaaaactca
    38341 aatacgtcag tcgtggtgga ttaaaactgg aaaaggggct gcacgttttt ggtgtatcag
    38401 ttgctaatca aattgggatt gatattggcg cttcaacagg tggttttacc gatgttatgt
    38461 tacaagatgg ggccaaacta gtctatgccg tcgacgttgg gactaaccag ttggtatgga
    38521 aactcagaca agatccacga gtaagaagta tggaacagta taactttcgt tatgctcagc
    38581 cagaagactt taatgaggga cagcctgtat ttgccagtat tgatgtgtcc tttatttctc
    38641 tcagcttgat tttgccagcc ctgcataacg ttttgtcaga tcagggacag gttattgctc
    38701 tcattaaacc gcaatttgaa gctgggcgcg agcagattgg taaaaaaggc atcgtcaagg
    38761 acaaacagat tcatgaaaaa gtgattcaaa aggtcatgga ttttgcctca ggttacggat
    38821 ttacagttaa gggacttgat ttttctccta ttcagggtgg tcatggcaat atcgaatttt
    38881 tagctcatct tgctaagtca cagacacctg aaacgttagc cccgcatttg attcagaaag
    38941 ttgttgccaa ggcacataag gagtttgaga aacatgaaaa agagtgaacg acttgaatta
    39001 atcaaaaaaa tggttttgac gcatccaatt gagacgcaac atgacctctt aagattattg
    39061 gcagagcatg ggttagaatt aacccaggct actatttcaa gagacatgaa tgaaattggt
    39121 atagtaaaga ttccttctgg cagtggacgt tacatctatg gtctttctca agatagtgga
    39181 aaaaagatcg ttcaaggacc tagatcgata aagagcacta ttttagctgt atctgacaaa
    39241 acaaaaggtt tagaacaaca cctctattta aaagtcgtac ctggcaatag taaattaatc
    39301 aaacgttatt tattagcaga tttttcaaaa gctattttta gtcttattgc tgatgatgat
    39361 agtttattgt taattgcaaa gtctccttca gaagcagata tgatacgcca agaaattttg
    39421 ctttggatgc agggtataac ctagaaaaaa ataggggaga aagtgatgtt attagaaatt
    39481 tctattaaaa attttgctat tattgatgaa atttccctaa attttgaaaa tggtatgacg
    39541 gttttgacgg gtgaaactgg agcaggaaag tctattatca ttgatgctat gaatatgatg
    39601 cttggtgcgc gtgctagtac agaagtgatt cgtcgtggag ctaataaagc agaaattgag
    39661 gggttctttt cggtagatgc taccccagaa ctggttgcgt gtctggaatc atcaggtatt
    39721 gctatggaag aagaactgat tattcgccga gatatctttg ccaatggcag aagcgtgagc
    39781 cgcattaatg gtcagatggt taacctagcc accttaaaac aggtcggaca gtttttggta
    39841 gatattcatg ggcaacatga ccaagaagaa ttaatgcgac cacagctcca ccagcaaatt
    39901 ttggacgctt ttggtgataa ggcttttgag caattgaaag agaattatca gcttattttt
    39961 gatcgttata aaagcttgcg tcgccaggtt attgacaaac aaaaaaatga aaaggaacac
    40021 aaagatcgta ttgatatgtt ggcctttcag atagcagaaa ttgaagctgc tgccttgagt
    40081 cgaggtgagg acgaccggtt aaatcaagaa cgtgatcgct taatgaacca caaacaaatt
    40141 gctgataccc tgaccaatgc ctacgtcatg ctagataatg acgatttttc aagtctatcc
    40201 aatattcgct ctagcatgaa tgacttacta tcaattgagc agtttgattc agagtacaaa
    40261 gggatgtcga cttcgatttc tgaagcctat tatattcttg aagaagtgag caagcaatta
    40321 tcagatacca ttgaccaact ggattttgat ggtgggcgat tacaggaaat tgaatttcgc
    40381 ttggatatac tcaatagctt aactcgtaaa tacggtggta atgtgaatga tgtgcttgac
    40441 tactacgata atatcgtcaa agaataccag ctattaacag gagatgactt gtcttcaggg
    40501 gatctagagg cagaattgaa aagcttggaa aaacaattgg ttgctgctgc ttcggagtta
    40561 agtgtctctc gccatcaatt ggcggagcaa ttagaagctg agattaaagc tgaacttaag
    40621 gaactttata tggaaaaagc agatttcaaa gttcacttta ccacatcaaa atttaatcgt
    40681 gatggtaatg aaagcttgga attttacatc tctactaatc caggcgaagg attcaagccc
    40741 cttgtcaagg tggcttctgg aggagagtta tcacgcctta tgttagccat caaggctgct
    40801 atttctagaa aagaagataa gacgagtatt gtttttgatg aagtagatac aggtgtttct
    40861 ggtcgcgttg cgcaagccat tgcccaaaaa atttataaaa ttgggcgaca tgggcaagtt
    40921 ctcgctattt cacacttacc tcaagttatt gctattgctg attatcagta ttttatttct
    40981 aaagaaagta aagaagagtc caccgtttct aaggtaaggt tattaactcc tgaagaacgt
    41041 gtggaagaaa ttgctagcat gattgcagga acagatatga cacaagctgc tcttacgcag
    41101 gctcgtgaac tacttgccaa acattaagat tatgtttggt atttgatatt ttcataatta
    41161 gatgacattg attttttagg tgctaaaata atcattgtgc tagaccattt ttcctctgtt
    41221 aatctggctt ggtttgcctt gacaaacctc tgagaaatag tagaatagat aagagaattt
    41281 taagctgagg ggatagaaat gactagtaaa aaagcgtgtt tatcaagcat cattgtgtta
    41341 gcaagtttaa cgtgtggaaa tgatactgtt agtgccaatc atctctcagc aactggagat
    41401 aagtttgatg attgctcaac acttgttgaa aaagatgtgg cccctaaaga tgaacttgag
    41461 atgttagcat ggtcctcgtc tcaaacaact gatgatgctg acagagacta tgaagatttt
    41521 ctcgatgatg attcttttat ttctcaaaat gaaactgata agatgtttga gaatttaact
    41581 gatgataggt tattaaatga attagatgaa ttagatgaag aaaatgaaga agatgaagaa
    41641 gatacaattg agccagagca aaatgtaata atgcctagtg acgatgagct atttgattta
    41701 actgatgctg ttgagacacg ccttactgtt tctagtgctc cccatttaga ggctgaattg
    41761 ccgaaaccac atttgaggag cctatcagat acagcactgc ggtctggtga aattagagga
    41821 catttagata acaaactgga cgctttgtct gtaacagcta caaagttagc attaacgatg
    41881 gctcaaaaat ttgatttgac aacgcatgtc tattctatag gtgaaagctt tagtgaagta
    41941 ttagctgctc attatgaaga cagaaaagca gaatcagctt tttctaagaa aaagagattt
    42001 caccttccta ttgctactcc agatgttgtt atagaggagt taaggcgcct agtctcttct
    42061 attggaagtt caaaagaaga tgtttcagtt ccttatagtc ggaagctagg tatggcagtt
    42121 gcaaaaagaa aaatagccct gccacaaacg ggagaggggt tctcttatta tccagtttta
    42181 cttggtttaa tgatattagg attaacgccg attatgatac caaagaagat aaataattag
    42241 gtaataagtt attgtagttg taaaagacgg ggcaaatgtt caagcgtgaa aaggttgact
    42301 tttgcctcgt tttgtatctc gttattgcta aggcattttc ttcgagcagc tagtgctcgc
    42361 catacctatc taccgagtga ttattcaata gcactaccag ttttgcttta tttagtaagg
    42421 agtgttagaa taaagaggtg agaaactagt ttagaatgga aaaatacatg ggaactataa
    42481 aaattgttac agattcatca ataactattg aaccagaatt aattaaagct ttagatatta
    42541 ctgtagtacc tttatctgtc atgattgata gcaaattata ttctgataat gatttaaaag
    42601 aagaaggtca ttttttgagc cttatgaagg ctagcaaatc ccttcccaaa accagtcagc
    42661 caccggttgg tctctttgct gaaacttacg aaaatttggt caaaaaagga gtaactgaca
    42721 ttgttgctat tcacctctca ccagctttat caggtactat tgaagcttcc cgtcaggggg
    42781 ctgaaatcgc tgaggcacct gttacagttt tagattcagg atttactgat caagccatga
    42841 aatttcaggt cgttgaggca gcaaagatgg caaaagcggg tgctagtttg aatgagattt
    42901 tggctgctgt tcaagctatc aaatcaaaaa cagagctcta cattggtgta tctaccttag
    42961 aaaatttagt aaaaggaggt cgtattggtc gtgtgacggg tgttttgagc tcgctactta
    43021 atgtcaaagt tgttatggca cttaaaaatg atgagttaaa aacgcttgtc aaaggtcgag
    43081 gtaataaaac atttacgaaa tggttagata gttacctcgc taaaaatagt catcgcccga
    43141 ttgcagagat agctatttct tatgctggtg aagctagcct ggcattaacc ttaaaagaaa
    43201 gaatcgcagc ttattacaac cactctattt cggttttaga aacaggctct atcatccaaa
    43261 cacatacagg agaaggagct tttgcggtta tggttcgtta tgaataatcg tcatttattt
    43321 agtgggatat ttttctttgt tattagttta tgtctggcct ttttattgct aaatattatt
    43381 atccctaagt caaattcacg tttgaaaaag agtgattttc tgaaaaaaga acaagtagct
    43441 atccaatatg ttgctatagg agattcattg acagaaggag taggtgatct aactcatcaa
    43501 ggtggttttg ttcctttgtt aacgaatgat ctcagtgaat attttaaggc taatgttaat
    43561 catcaaaatt acggcgtatc tggtgatacc agtcaacaaa ttcttgatag gatgataaaa
    43621 caaaagcaga tacagttatc tttaaaaaaa gcagatataa tgacgttaac cgttggtggt
    43681 aatgatgtta tggcagttat tcggaagaat ttagcggatt tgcaagtttc tagttttaga
    43741 aagccagctc gtcagtatca aaaacgatta agacagatta tcgagttagc cagaaaagat
    43801 aataaagatc ttcctatttt tattttaggc atctataatc cgttttattt gaattttcca
    43861 gaactaactg atatgcaaaa agtgattgat gactggaata ccaaaactaa ggaggttgtt
    43921 ggagaatacg atcgtgtgta ctttgtgcca ataaatgacc tcttgtataa agggataaat
    43981 ggacaagaag gaattgttca ttcttcagga gatcaaacta caattgtcaa tgatgccttg
    44041 tttactgggg accattttca cccaaataat actggctatc aaatcatgtc aaatgcagta
    44101 atggagaaaa ttaaaaagca tgaaaaaaaa atcaaacctt aattggtgga agtggagctt
    44161 cttatgtctg cttgctttta ataccgcctt tttgatggtt attgcaagtc gtctcattca
    44221 agtaagagaa cctgagtcag aactgattgc taaaaaacca gttaaaaata tcaaaattgg
    44281 tacatttgtt acaaccagag aacagcttaa cgaaactgta gcaagttatc ttaaagatta
    44341 tcagactgaa aaaatgtctt ataaatttta tgcgacatct tcttctattt tgtttgaagg
    44401 aacttatcaa ttattagggt atgaagtccc tttatatatc tattttcaac ctcatcgtct
    44461 agaaaatgga gctgtccaac tacaggtgat ctctttttca gtgggaactc tgccactccc
    44521 agaaaaagat gttttgcagt atttgaaatc aagctacaaa ttgccaagct ttgttaaagt
    44581 gatgccaaat caatcagcta tcgttgttaa tttacaagat atccaaaatg atgctaaggt
    44641 ttatttaaaa gccaagaaaa ttgatttatt taatgatgaa atcagtttta acatctataa
    44701 gaaataagct caaataacgt taaatacttg acctaatcgc tttttttaga tatgataata
    44761 tctgttaaag cattaagctt actaattatt tggaggattt gttaaatggc taacaaacaa
    44821 gatttaatcg caaaggttgc agaagcaact gaattgacta aaaaagattc agcagcagca
    44881 gttgatgccg tgttttctac aatcgaagct ttccttgctg aaggtgaaaa agtacaattg
    44941 atcggttttg gtaacttcga agtacgcgaa cgtgcagctc gtaaaggtcg taacccacaa
    45001 actggtgcag aaattgaaat tgcagcttca aaagttccag ccttcaaagc tggtaaagct
    45061 cttaaagacg ctgttaaata attgcataga taaaagccca ttgtatcaag cgttccagcc
    45121 tgtgcagtgg gcttttttgt gttcaggggg gtaaaaaagg ggtaaaaaat ttaatagttt
    45181 tccataatat cagctacact tgatttcatt ttctttgtga catgcgtata aatttgggca
    45241 gtagttttag cgtctgcatg tcctacacgt tccatgattg attttagcgg cacgttattc
    45301 tctgctagac ggctaataag agtgtgacgg aagatgtggc tagtaagttt ttttgtaatt
    45361 gggtcttcca atctttcatt agcttttttt aaagctaagt tgaatgagtt agtctgaatt
    45421 ggaacaccgt tttttgttgt aaaaaggtat cccatatctc tatatctatg atttgtattt
    45481 ttttctagtt cgttcataaa ttctaactct tgtagaattt caagttctct acttgtcatg
    45541 atggtttcac gataagaagc taatgtcttt ggtgctgtct tttccccgtt acgatatcct
    45601 tctgtatgat caaatgttcc gtgcaactgc aatattcttg acttagattc gtagttgcac
    45661 ggctcaatgc tgacagcttc accgatacga caaccgttca aactgataaa ctcagccaac
    45721 aaaccaagac ggtaagtgct aggtcttctg tacaattctt tcaataatgg tttaatttca
    45781 tcttcttcaa gatatttttc ttcagccttt ttccagtctt caagagtttt ctttaccctt
    45841 gggagtttag cacgtcttgc aggattatct tggataatat ccaaaccaac agcataatca
    45901 aatgctaagt ttagcataga cttattacgc tcttttttat tcctagagca atctaggtta
    45961 tcgagataat tctgaacgta tttaggatca atattcacta ctttaacacc tattccaaaa
    46021 gtttccctta tctcttcgat gttacccctc agagaagcta cagacgacct tttaagttct
    46081 ttcttataaa acgcccacca ttcatctaaa agttccgtga aaagtaactc agaggccttt
    46141 aattgcgcta ttttttctgt tattttttgt tcaaggattt ttcgagctgc tttataagct
    46201 cttgaattat ccttctccat agtaacggat acaggtttcc atgttccagt gtaaggatct
    46261 ttataatttt cacgaaattt gaatttaccg ttatcagttt cttctatcca catttgtttt
    46321 ttacctcatt ttttgttaaa atgagtataa gaaaacgacc tatttaatgg ttgtttctta
    46381 tactagatcc gcctcacgct cgcaccgacc aaagttgaac gtgtggcttt tttattttac
    46441 ttgattttct caaataccat tgtagcttgt attctatctc cgccaccaaa tcctttactg
    46501 ccaccatttg atgtggtaat agtgtgtagg cgatatcctt tttttgcttg cttattaata
    46561 acattttcaa gttcagttaa atttcctgag cctgttccta taagcttttc ttttagtgta
    46621 acttgtagca caacatattc ataattttcg ccagaagcag ttgaaacagt actagcttct
    46681 tttacattgt ccaaaaatcc catgataatc tcctttaacc tattaaagta taatattcat
    46741 caataaccat gacttcatcg gtcgtggtct ttaaattatg tctttgcata aaagacaagt
    46801 agttaaactc atgtttgtct tctactttgt caaattcatc ttcaagcaaa cacctgatca
    46861 tatgtctatt tgcctgcaat tcaaattctt catgacgcct ttgatattgg ccagaatcat
    46921 gttcaatatg tcctagttca tgaaaaataa ctttcttttt tgactcatcg gataatgctc
    46981 tattaacgaa gacaactttt atttcatcaa tatagattcc tggacgtggc catagttcat
    47041 tatcgaaata agctaattct acattatgga agtctaaaat ctcctcaata gtcatagact
    47101 atcttccctt caagtaaatt tcaataatat tttgtattgc ttcaatatct gattcagtaa
    47161 gcggcttacc atcgaacgtt ttggcgtttt tggccatttt tcgtagatct tcagaagtgt
    47221 attcttggtt atcttttgca attgcagggt tatcagtgcg accaagcaag taatctgttg
    47281 agacgtggaa atagtctgca atctcttgta atttttcaga acctggtttg tttcgtttta
    47341 aagcatagat ggtatttcga ctatacccca tcttttcttc tagagaattt attgaaagac
    47401 cttgtttttt agcaagttct tttatccttt caaatgttat aaacactgat ttatcaacct
    47461 ttctggagga tgacaaaaaa tattttaaag aatttcaata aaaaccgttg actttttacg
    47521 gagaatactc taaaatagtt tttgtaagat agttagttag taaaaaacga agttaaacat
    47581 tttacaaatc tttttaaagt agttggggaa ccgcgtaata taaaagagtt ataaacgtta
    47641 taactatgtt tttcttatg
//
LOCUS       DAXWIQ010000011        47176 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000011 DAXWIQ010000000
VERSION     DAXWIQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47176
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.011"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            28..1890
                     /locus_tag="ACGEUI_000591"
     CDS             28..1890
                     /locus_tag="ACGEUI_000591"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8165449.1"
                     /translation="MTIRQWMADHLHLMETLACLVLIIIGLAFLHSFPQVASAIFITA
                     FLIGGYASAKTGILDLVKNKHLSVDILMILAAIGAGIIGYWLEGALLIFIFSLSNTLE
                     EMAMEKSKDAISALMSLTPDTARQYQEDGHILEVETRSLSVGDRLQVRKGEAVPIDGQ
                     LLSPFGQFDESMVTGEPITVDKAEGQDLIGGTINQGQTIDMLVTIENDDTLFAKIINL
                     VESAQEKKSKTATFIESLEDGYVKFVLVLIPAFILFSHFVLSWTWLAAFYRGMILLTV
                     ASPCALIASSTPASLAAISRAARKGLIIKGGDIVDNMGDIKAVVMDKTGTLTQGKPSV
                     VNAHYLEDELLVNRLVKGAETASTHPISKALLEYTEKLEPLTFDHLEEISGKGFQGFY
                     QGQEWRIGKKTYILEKVQDLSAFEETIQVEEKQGKTLIFVSRDHQLIAYYALLDDIKI
                     ESKRAIKSLHAMGIKTVMLTGDQERTANYVAQKLGIDEVVANCMPQDKVAKLAELKTK
                     YGFVAMVGDGINDAPALAQADVSYAIGSGTDIAMESADSVIMDDLTRIPFSIQLSRTM
                     KTIIKQNIVFALSVITLLILANVFQVVNLPLGVVGHEGSTILVILNGLRLLSFK"
     gene            complement(2190..3125)
                     /locus_tag="ACGEUI_000592"
     CDS             complement(2190..3125)
                     /locus_tag="ACGEUI_000592"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFF8165450.1"
                     /translation="MVSTATQIGHFSFDNCLMNAAGVYCMTKEELMEVEKSQAASFVT
                     KTGTLEVRPGNPEPRYADTRLGSINSMGLPNNGFRYYLDFVSDLAKTGQHKPHFLSVV
                     GLSPTETETILKAIMASDYEGLVELNLSCPNVPGKPQIAYDFETTDQLLENIFTYYTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLSFVNCVNSIGNGLVIKDEQVLIKPKNGFGGI
                     GGDYIKPTALANVHAFYKRLKPSIHIIGTGGVKTGRDAFEHILCGASMVQIGTALHQE
                     GPAIFERVTKELKTIMVEKGYQSLDDFRGNLRYKD"
     gene            3364..4059
                     /locus_tag="ACGEUI_000593"
     CDS             3364..4059
                     /locus_tag="ACGEUI_000593"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HFF8165451.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLTRAIKTTNLALENAGQLWVPTEKSWRLNERHYGALTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPAMAKDDEYSAHKDRRYADLDPALIPDAENLKVTLERAM
                     PYWEEKIAPALLDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFELDE
                     KLNIVKEYYLGGE"
     gene            4166..4687
                     /locus_tag="ACGEUI_000594"
     CDS             4166..4687
                     /locus_tag="ACGEUI_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFF8165452.1"
                     /translation="MKAEDRRQKIIECLNSEQKAVSATRLGKLLGVSRQVIVGDIALL
                     RAQQIDIISTPKGYIMSTALYSHQFCARIVCQHNVEETKKELEIILAHQGIITTVEVE
                     HPIYGMITAPLNIKTHSDVTNFMSKLSQSKAELLSSLTEGLHSHLISCPSQEAFLAIK
                     HDLELAGILYKGI"
     gene            4848..5390
                     /locus_tag="ACGEUI_000595"
     CDS             4848..5390
                     /locus_tag="ACGEUI_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165453.1"
                     /translation="MTKKPTQLIAYTSILVAFAILIPIIMPLKLIIGPASFTLASHVP
                     LFLAIFMSIPVAILVALGTTLGFLLAGLPLIIVLRALSHLLFAILAAWWLSRKPQLMT
                     SAVKCFSFAFFINVIHGLAEFLVVYILTATTATSMSYFWSMLGLIGLGSLIHGILDFY
                     LALVLWRFLAKNLKLPTINA"
     gene            5514..6293
                     /locus_tag="ACGEUI_000596"
     CDS             5514..6293
                     /locus_tag="ACGEUI_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFF8165454.1"
                     /translation="MKTPEQILEATIHIGEHKVTKTFLAKSILGFIGGAMISLGYLLY
                     VRIAASGLETFGAFSSIVGACAFPIGLIIILMAGGELITGNMMAVSAALLAKKIKFSE
                     LAKNWLIITLFNVIGAVFVAFVFGHFLGLTSAGIFKEEVIEVAHAKIAASPLQALVSG
                     IGCNWFVGLALWLCYGANDAAGKFLGTWFPVMTFVALGFQHSVANAFVIPAAIFEGGA
                     TWLDFVTNFIFVYSGNIIGGAIFVSFLYFKVYYHPQKSKTQ"
     gene            6854..7045
                     /locus_tag="ACGEUI_000597"
     CDS             6854..7045
                     /locus_tag="ACGEUI_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HFF8165455.1"
                     /translation="MANPNGSVTIHAKIGTVETCITDNSDQTLVTYNLNVVAYNTNNE
                     DNIAVAVMYPHITNISAKM"
     gene            7092..7688
                     /gene="recR"
                     /locus_tag="ACGEUI_000598"
     CDS             7092..7688
                     /gene="recR"
                     /locus_tag="ACGEUI_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFF8165456.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSNEDVNDFA
                     KNLLAAKRELTYCSICGNLTDDDPCHICTDTSRDQTTILVVEDAKDVSAMEKIQEYHG
                     YYHVLHGLISPMNGVGPDDINLKSLITRLMDGKVSEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            7789..8835
                     /locus_tag="ACGEUI_000599"
     CDS             7789..8835
                     /locus_tag="ACGEUI_000599"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFF8165457.1"
                     /translation="MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFIT
                     QMGQFIKTQQFSEKPSESERLMTNETIELTQKIKPSDIYEEGAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVE
                     TLARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEV
                     GLLGNDKVKSTLPGEVIKDVDFYDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAF
                     KALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWENMGLAYPDLIEELVT
                     LAQEMFDQRESHLI"
     gene            9026..10417
                     /gene="murF"
                     /locus_tag="ACGEUI_000600"
     CDS             9026..10417
                     /gene="murF"
                     /locus_tag="ACGEUI_000600"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFF8165458.1"
                     /translation="MKLTLHEVAKIVDAQNNVSDLDDVPLHHIEFDSRKITKGDLFLP
                     LKGQRDGHEFIDLAFQNGAVATFSEKELPGKPHLLVEDCLKAFQKLAHYYIDKMRVDV
                     IAVTGSNGKTSTKDMIGAVLSTTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKIVLEMG
                     QDHMGDIRLLSEIARPRIAVLTLVGEAHLEYFGSRDKIAQGKMQIVDGMNSDGILIAP
                     GDPIIDPYLPENQMVIRFGNQQEIDVTGIQEDKDSLTFTTNVLATPVSLPLPGKYNAT
                     NAMVAAYVGKLLAVTDEDIIAALQTVTLTGNRTEWKKAANGADILSDVYNANPTAMRL
                     ILETFANIAKNPGGKKIAVLADMKELGKDSVILHSQLIDSLTSGNIDQLVFYGDHIKE
                     LARLASQVYPAEQVHYFLKTEQEDQFEAMAQYVQNILNPFDQILLKGSHSMSLEKLVD
                     RLVTDKNCDSYQP"
     gene            10497..11192
                     /locus_tag="ACGEUI_000601"
     CDS             10497..11192
                     /locus_tag="ACGEUI_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HFF8165459.1"
                     /translation="MDFFAIDPIGLPHTSLIFYLSSLLIALLFVFLTFQAYRLKSYRY
                     FFLFLQLSQVIGLYTWYVLRGFPLDEALPLYHCRIAMLAIFFLPDRNKFKQLFMVLGI
                     GGTFLALLSPDLYPFRLWHVANVSFYFGHYALLVNGLIYLLRFYDASQLRLLSVVRYL
                     ATVNFLLLLVSLATKGNYGFVMDIPVIHTRHLLLNFVIVTSGLTFMVKITEYFYLKFG
                     EAQQLALAFSKEK"
     gene            11440..12984
                     /locus_tag="ACGEUI_000602"
     CDS             11440..12984
                     /locus_tag="ACGEUI_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFF8165460.1"
                     /translation="MSLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLG
                     IASYPMNWPIGMGRAFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VVDPLAKDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     PKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWISEDDLDQRMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     TLEEKM"
     gene            13291..14910
                     /locus_tag="ACGEUI_000603"
     CDS             13291..14910
                     /locus_tag="ACGEUI_000603"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8165461.1"
                     /translation="MKFTEFNLSQDIQSAVVTAGFEKASPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIRTNENIIQALVIAPTRELAVQSQEELFRFGREKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALILDHVETLILDEADEMLNMGF
                     LEDIEAIISRVPADRQTLLFSATMPAPIKQIGVKFMKDPEHVQIKNKELTNVNVDQYY
                     VRVKEQEKFDTMTRLMDVNQPELSIVFGRTKRRVDEITRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDILVATDVAARGLDISGVTHVYNYDITQDPESYVHRIGRTGRAG
                     KSGESITFVSPNEMGYLSMIENLTKKQMKPLRPATAEEAFQAKKKVALKKIERDFADE
                     TIRSNFDKFKGDAVQLAAEFTPEELALYILSLTVQDPDSLPEVEIAREKPLPFKYVGG
                     GHGNKNGKGGRGRDNRNRGDRRGGYRGDRNRDERDGDRRRQKRDKRDGHDGSGNRDFK
                     RKSKRNSKDFFNKEKKSSAKNTGFVIRHKGE"
     gene            15038..17038
                     /locus_tag="ACGEUI_000604"
     CDS             15038..17038
                     /locus_tag="ACGEUI_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFF8165462.1"
                     /translation="MSDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTIQSLVENQG
                     GVNQVSESFILGSISLIIWTLTLITTIKYVLIALKADNHHEGGIFSLFTLVRKMSPWL
                     IIPAMIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLF
                     GIQRFGTGFIGKIFGPVMFIWFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENH
                     RGIFILGSIFLATTGAEALYSDLGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKH
                     SGIELNPFFASVPSQLRVYLVSLATLAAIIASQALISGSFTLVSEAMRLKIFPLFRVT
                     YPGANLGQLYIPVINWILFAVTSCTVLAFRTSAHMEAAYGLAITITMLMTTILLKYYL
                     IKKGTRPILAHLVMAFFALVEFIFFLASAIKFMHGGYAVVILALAIVFVMFIWHAGTR
                     IVFKYVKSLNLNDYKEQIKQLRDDVCFDLYQTNVVYLSNRMQDHMIDRSILYSILDKR
                     PKRAQVYWFVNVQVTDEPYTAKYKVDMMGTDYMVRVNLYLGFRMPQTVPRYLRTIVQD
                     LMESGRLPKQEQEYTITPGRDVGDFRFVLIEERVSNARQLSNFERFIMQTKASIKHVT
                     ASPMRWFGLQYSEVTLEVVPLILSDVLKLPIKELVPVEDSEA"
     gene            complement(17062..17340)
                     /locus_tag="ACGEUI_000605"
     CDS             complement(17062..17340)
                     /locus_tag="ACGEUI_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFF8165463.1"
                     /translation="MTTKKAYMYVLECVDKTLYTGYTTDLKKRLATHNAGKGAKYTRY
                     RLPVSLLYYEVFDSKEAAMSAEALFKKRKTRSQKLAYIATHQKEKKNH"
     gene            complement(17330..18106)
                     /locus_tag="ACGEUI_000606"
     CDS             complement(17330..18106)
                     /locus_tag="ACGEUI_000606"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFF8165464.1"
                     /translation="MIKAILKEGERIDQLFSSDVGIIQNKDVFSYSIDSVLLSRFPKM
                     PSKGLIVDLCSGNGAVGLFASTRTKAAIVEVELQERLADMGQRSIQLNQLEDQVTMIC
                     DDLKNLLNHVPRSGVDLMLCNPPYFKSHESSKKNVSEHYLLARHEVTTNLEEICQVAR
                     HALKSNGRLAMVHRPDRFLEIIDSLRANGLAPKRVQFVYPKLGKSANMLLIEAIKDGS
                     IEGMTILPPLVVHKENGEYTDHIFEIYFGAASKGKPNHDH"
     gene            18224..18964
                     /locus_tag="ACGEUI_000607"
     CDS             18224..18964
                     /locus_tag="ACGEUI_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFF8165465.1"
                     /translation="MFYAYLRGLVVFLLWVVNGNAHYHHEEKMLDASENYILVAPHRT
                     FWDPVYMAFAARPKQFIFMAKKELFANRLFAWWIKMCGAFPIDRDKPSPDAIRYPVNM
                     LKKSNRSLLMFPSGSRHSQEVKGGVAVIAKLAKVKIMPAAYQGPMSVKGLLAGERVDM
                     TFGNPIDVSDIKRMNDEGIAEVANRIQAEFDRIDDELAPFQPGKARNPLTYLYRLPLG
                     LVLVVVLLLTMLFSYIASFVWNPDKHRH"
     gene            19164..19826
                     /locus_tag="ACGEUI_000608"
     CDS             19164..19826
                     /locus_tag="ACGEUI_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFF8165466.1"
                     /translation="MLDELLIKGKSFLRERYFLPYLISLLLGLFLILSFSFLFWGNRQ
                     SKAAVPALREISPVKQQVSEEKKEIQEDSSILVDLKGAVQKEGVYKLTASSRVRDVIE
                     LAGGLTSEADKHAINFAEKLTDEQVVYVPKQGEEISVLPRSLVSGKKETASKDQSKVH
                     INKASLEELQHIPGIGAKRAQDIIDMRDKLGGFKALEDLRQVSGIGEKTLEKLKDDIF
                     LD"
     gene            19807..22050
                     /locus_tag="ACGEUI_000609"
     CDS             19807..22050
                     /locus_tag="ACGEUI_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFF8165467.1"
                     /translation="MTFSWTKLVPLSKIQFAFLILVFFYQIHSPSWLTFLLSLSLICL
                     LVKRLSKKEFLGVFAILSFCALFLLYQKQQLVQKLEIQPVQITSVALVPDSIRINGDQ
                     LAVLGRHGKHSYQLFYRLKSQAEAQLFKKEHRWLVMHAKVTLEKAEEVRNFKGFNYQT
                     FLTYQGIYRIGKVEQIEQLEVISPESICDYLSSLRRRAIVHCQQHFPRPMSHYLTGLL
                     FGYLDKSFGEMTDYYSQLGIIHLFALSGMQVGFFLTCFRRVLLLLAVPLEWIKWIELP
                     FACFYAALTGYSISVIRSLVQSQLRHLGIKGLDNLACTFLLVFLWDAHFLMTVGGVLT
                     FSYAFLLTVVTVEELSGAKRQLVQVLTISLGILPFLLFYFSSFNPMSMVLTGLLSYLF
                     DLFILPLLCLVFCLSPLVTVSICNHLFILLEKVIQFLGNTFNSSLVFGSPTSWHLLIL
                     VISFAIFYDYRQVRQRVITCGLVIALTLLSVKYPLTNEVTFIDIGQGDSILVREWTGK
                     NLLIDVGGRPFFSSKEHWRRGHHVANAQKTLIPYLKSRGIHTIDQLLVTHADTDHMGD
                     IEVVAKAIRIKEILTSQGSLSHPSFVRRLRRLKCHVRVLAAGDQLPIMGSVLQVLYPW
                     QLGDGKNNDSLVLYGRLLNRTFLFTGDLEKEGENEIIKRYPQLRVDYLKAGHHGSNTS
                     SSAAFLDHIQPKVAFISAGKNNRYQHPHRETLARLEDRQITYYRTDTQGAIRLTGRTS
                     WHLETVR"
     gene            22121..23161
                     /gene="holA"
                     /locus_tag="ACGEUI_000610"
     CDS             22121..23161
                     /gene="holA"
                     /locus_tag="ACGEUI_000610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFF8165468.1"
                     /translation="MIAIEKIEKLSKENLGLITLVTGDDIGQYSQLKSRLMEQIAFDK
                     DDLAYSYFDMSEAAYQDAEMDLVSLPFFAEQKVVIFDHLLDITTNKKSFLKEKDLKAF
                     EAYLENPLETTRLIIFAPGKLDSKRRLVKLLKRDALVLEANPLKEAELRTYFQKYSHQ
                     LGLGFESGAFDQLLLKSNDDFSQIMKNMAFLKAYKKTGNISLTDIEQAIPKSLQDNIF
                     DLTRLVLGGKIDAARDLIHDLRLSGEDDIKLIAIMLGQFRLFLQLTILARDVKNEQQL
                     VISLSDILGRRVNPYQVKYALKDSRTLSLAFLTGAVKTLIETDYQIKTGLYEKSYLVD
                     IALLKIMTHSQK"
     gene            23258..23863
                     /gene="sodA"
                     /locus_tag="ACGEUI_000611"
     CDS             23258..23863
                     /gene="sodA"
                     /locus_tag="ACGEUI_000611"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodA"
                     /protein_id="HFF8165469.1"
                     /translation="MAIILPELPYAYDALEPQFDAETMTLHHDKHHATYVANTNAALE
                     KHPEIGENLEELLADVTKIPEDIRQTLINNGGGHLNHALFWELLSPEKQDVTPDVAQA
                     IDDAFGSFDAFKEQFTAAATGRFGSGWAWLVVNKEGQLEITSTANQDTPISEGKKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEIVNWKKVSELYQAAK"
     gene            24030..24257
                     /locus_tag="ACGEUI_000612"
     CDS             24030..24257
                     /locus_tag="ACGEUI_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165470.1"
                     /translation="MLRKKKTTIVGIILGMMIVTLLVGCLTGKKKGFPPKAGERQQTA
                     DLKMRKRLEKLFKNRYLKNLKRLSKLLWMQI"
     gene            24242..24490
                     /locus_tag="ACGEUI_000613"
     CDS             24242..24490
                     /locus_tag="ACGEUI_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFF8165471.1"
                     /translation="MDADLAQMDVLGLYYDDNHLSLVNTVKAYLKEFGIGESQIAFSY
                     KDLNSGRTAAMNDHQPMIAGSTYKLPLNMLVVDKANYP"
     gene            24487..25077
                     /locus_tag="ACGEUI_000614"
     CDS             24487..25077
                     /locus_tag="ACGEUI_000614"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFF8165472.1"
                     /translation="MTERFDITETDYEYDQEHHAYVAQFNGAMSIPDMQEYSLVYSEN
                     TPAYALAERLGGMNKAYQLFDRYGKVSGAITTIDRNGNKITTAYYLQVLDYLWQHQDK
                     YKDILYYIGESFPDLYYKTYLPHVKVYQKPGYVREALNVGAIVCEESPYLIALYSSGL
                     GGATQASEEVNGLGYVQLVQLPYVINEWYRGNLNMI"
     gene            complement(25191..26411)
                     /locus_tag="ACGEUI_000615"
     CDS             complement(25191..26411)
                     /locus_tag="ACGEUI_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HFF8165473.1"
                     /translation="MKLLGKWLFFLMFLTFCVSFWQKDYQRIAFEKEHKLYQDLVAPF
                     YQNYPKLRELQLSEAIKLRHDLLDYVRKLDKDGWSYKAIQKGYLEQLAVRGNSYHFEQ
                     LYSRIRLIGSPDFQKLWQQEEMAQTPQEAQKRLQLLLTYLKMPDELTGQSKQTQQVLA
                     QLSPKLSPTDPFWDQLSTLIQAYYVNLEHIPYSRFNRKIHQLRYLISTQQTEWVRSHY
                     GNKGKTDADALAKYLATLEDDDYALYESSRYHNKVASILDANGNHQAVYTDNIPQANY
                     KILIHFHSEFILSESGQFLVALDPDNLTRNSIVNGSSFNYGNQNDDLHRLLDIDPILL
                     FDPAFIEKAINSPDATFLVPDLEQQRDKHNPIYSRNGKSSKQLTRAAVKKFKKLLHHY
                     QETRGDNKTSKTQH"
     gene            complement(26418..27446)
                     /gene="queA"
                     /locus_tag="ACGEUI_000616"
     CDS             complement(26418..27446)
                     /gene="queA"
                     /locus_tag="ACGEUI_000616"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFF8165474.1"
                     /translation="MNTNNFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTQGDQWEVLAKPAKRL
                     KVGSQVNFGDGRLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHDMHSEFYSLSEEAAQTLRDVKQAGGRVVAVGTTSIRTLETIGGKFQGDIQADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLEAYRHAVDEKYRFFS
                     FGDAMFVN"
     gene            27648..28352
                     /gene="nagB"
                     /locus_tag="ACGEUI_000617"
     CDS             27648..28352
                     /gene="nagB"
                     /locus_tag="ACGEUI_000617"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFF8165475.1"
                     /translation="MKIIRVQDQIEGGKIAFTLLKDSLAKGAKTLGLATGSSPISFYQ
                     EMVKSPLDFSDLTSINLDEYVGLSVESDQSYDYFMRQNLFNAKPFKKNYLPNGLATDV
                     EAEAKRYNQIIAEHPIDFQVLGIGRNGHIGFNEPGTSFEEETHVVDLQESTIEANSRF
                     FTSIEDVPKQAISMGIASIMKSEMIVLLAFGQEKADAIKGMVFGPITEHLPASILQKH
                     DHVIVIVDEAAASQLD"
     gene            28471..29187
                     /locus_tag="ACGEUI_000618"
     CDS             28471..29187
                     /locus_tag="ACGEUI_000618"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFF8165476.1"
                     /translation="MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPG
                     LQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLYPVGRL
                     DRDTEGLVLLTDNGPLGFRMLHPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
                     CQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLA
                     SGEYRYLTASERQLIKTYLD"
     gene            29482..30444
                     /locus_tag="ACGEUI_000619"
     CDS             29482..30444
                     /locus_tag="ACGEUI_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HFF8165477.1"
                     /translation="MTKILTALDDKNQLISLVTQPISTKPPFRCPACKSPVRLRQGTI
                     RRPHFAHVQLAHCQFQAENESEEHLTLKAKLYTSLVRTEAVCIEKYLPELQQIADLWV
                     NDKLALEIQCSPLPVERLKKRTKAYQEKGYPVRWLLGRKLWLNTHLTALQKQFLYFSS
                     SLGFHLWELDAAANLLRLKYLIHEDLFGKVSYLTKTISLDHNIMEMFRLPYQQEILYS
                     YQKKMTVNLSKRIQRALLARHPKWLRRQEKAYLSGYNLLMLTTDAFYPQWRPVQSSSG
                     FCQIKGNLRPYYESFKVYYKKEKDKKVQTLFSPKYYVKMDSNRK"
     gene            30457..32262
                     /gene="pepF"
                     /locus_tag="ACGEUI_000620"
     CDS             30457..32262
                     /gene="pepF"
                     /locus_tag="ACGEUI_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFF8165478.1"
                     /translation="MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEVEASKGFA
                     GHLLDSSANLLKVTKTYLELARRVEKVYVYAHMKNDQDTTVAKYQEYQAKASGLYAKF
                     SEVFSFYDPEVMMLHQEDYQAFLTETPELKVYNHFFDKLFQAREHVLSQAEEELLAGA
                     QEIFNGAEETFSILDNADIVFPVVKNDKGEDVELTHGNFISLMESKDRSVRQAAYEAM
                     YSTYEQFQHTYAKTLQTNVKVQNYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNK
                     HLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALEKAEKVLAVFGKDYA
                     DRVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFILLNWQDTLDNLYTLVHETGHSL
                     HSTFTRETQPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFR
                     GTVFRQTQFAEFEHAIHQADQKGEVLTSEYLNQLYADLNEKYYGLSKKDNHFIQYEWA
                     RIPHFYYNYYVYQYATGFAAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPLEVIAKA
                     GVDMEKGDYLEAAFKVFDERLTELEVLVSKGIHKS"
     gene            32638..33834
                     /locus_tag="ACGEUI_000621"
     CDS             32638..33834
                     /locus_tag="ACGEUI_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFF8165479.1"
                     /translation="MEKTKRYIIATAGILLHLMLGSTYAWSVYRNPILQETGWDQAPV
                     AFAFSLAIFCLGLSAAFMGNLVEQYGPRLTGTVSAILYASGNMLTGLAIDRKEIWLLY
                     IGYGVIGGLGLGAGYITPISTIIKWFPDKRGMATGFAIMGFGFASLLTSPIAQWLIET
                     EGLVATFYLLGLIYLIVMLFASQLIIKPTAAEIAILDKKRLQNNSYLIEGMTAKEALK
                     TKSFYCLWVILFINITCGLGLISVVAPMAQDLTGMSPEMSAIVVGAMGIFNGFGRLVW
                     ASLSDYIGRRVTVILLFLVSIIMTISLIFAHSSLIFMISIATLMTCYGAGFSLIPPYL
                     SDLFGAKELATLHGYILTAWAIAALTGPMLLSITVEWTHNYLLTLCVFIVLYILGLMV
                     ALRLKK"
     gene            33900..34607
                     /locus_tag="ACGEUI_000622"
     CDS             33900..34607
                     /locus_tag="ACGEUI_000622"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFF8165480.1"
                     /translation="MVKSYSKTANHNMRRPVVKEELVHYMRTRQKQTTGFLAELEQFA
                     RQENIPIIQPEVVAYFRFLLQSLQPKHILEIGTAIGFSALLMAENAPDATIVTIDRNR
                     EMIDFAKANFAKYDSRQQIRLLEGDAADILSTLEGNFDFVFMDSAKSKYIVFLPEILR
                     LLKVGGVVILDDVFQGGDITKPIEDIRRGQRTIYRGLQSLFDATLTHPNLTTSLVPLS
                     DGLLMIRKNQADIVLPD"
     gene            34670..35725
                     /gene="prsA"
                     /locus_tag="ACGEUI_000623"
     CDS             34670..35725
                     /gene="prsA"
                     /locus_tag="ACGEUI_000623"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HFF8165481.1"
                     /translation="MKNSNKLIASVVTLASVMALAACQSTNDNTKVISMKGDTISVSD
                     FYNETKNTEVSQKAMLNLVISRVFEAQYGDKVSKKEVEKAYHKTAEQYGASFSAALAQ
                     SSLTPETFKRQIRSSKLVEYAVKEAAKKELTTQEYKKAYESYTPTMAVEMITLDNEET
                     AKSVLEELKAEGADFTAIAKEKTTTPEKKVTYKFDSGATNVPTDVVKAASSLNEGGIS
                     DVISVLDPTSYQKKFYIVKVTKKAEKKSDWQEYKKRLKAIIIAEKSKDMNFQNKVIAN
                     ALDKANVKIKDKAFANILAQYANLGQKTKAASESSTTSESSKAAEENPSESEQTQTSS
                     AEEPTETEAQTQEPAAQ"
     gene            36112..38730
                     /gene="alaS"
                     /locus_tag="ACGEUI_000624"
     CDS             36112..38730
                     /gene="alaS"
                     /locus_tag="ACGEUI_000624"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFF8165482.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPDWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDAAAKVQALS
                     DSLRDLQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAEIV"
     gene            39091..39306
                     /locus_tag="ACGEUI_000625"
     CDS             39091..39306
                     /locus_tag="ACGEUI_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8165483.1"
                     /translation="MEVILKNRLKELRARDGINQTEMAKLAGVSRQTISLIERNEYTP
                     SVIIAMKIAKVFQEPVEEVFRLVEVEE"
     gene            39303..40064
                     /locus_tag="ACGEUI_000626"
     CDS             39303..40064
                     /locus_tag="ACGEUI_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HFF8165484.1"
                     /translation="MKKKKNSFLRLLKMSLLSSLAGGIIGGMVGAFLGYHGGRLDHLT
                     FLKDDVINLIILLNRLVVVTDLTLSFVFLTQLKKETAVYNTIEEDDISENGYRQLNKK
                     HAYTMLLIAVASILSMCNVLLGLTLTNDSQHAMLAIPLLDILLLLMVIPFQALAMKRY
                     NAIRGTDVPYFPNLKELKHNIMALDEAELQAYHKTSFESVLSLNGVIIPSLYVILFFV
                     YLFTGQVELTAILVLVLIQLYLLVKSATMTRQFYR"
     gene            40083..40778
                     /locus_tag="ACGEUI_000627"
     CDS             40083..40778
                     /locus_tag="ACGEUI_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFF8165485.1"
                     /translation="MKGFINYLKIAVLIILAMVFNVLPMILLQKQHDIPMVLNWGIGI
                     FYLVIVGSVLIVLWRLYQAKQDTSIKQQKMRLVDWGYLALFWLIIRVIAIVGTLVNQL
                     WSGQQVSANDAAIHTLARLIKGGFPLYTALFVLVIAFIAPIMEELVFRGFPMIDLFKG
                     KSLKVAGLVTSLVFALPHATNSVEFIMYSCMGIFLFVAYQRRGNLKDAILLHIFNNLI
                     EVILLMSIGLGVI"
     gene            complement(40870..42026)
                     /locus_tag="ACGEUI_000628"
                     /pseudo
     CDS             complement(40870..42026)
                     /locus_tag="ACGEUI_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558257.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(42164..43477)
                     /locus_tag="ACGEUI_000629"
     CDS             complement(42164..43477)
                     /locus_tag="ACGEUI_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFF8165486.1"
                     /translation="MTIIIMDSNSAHETDNLSVSFLNFCYNSLMKRHFLLLTFYLFLT
                     GLTAGLVAFILTKAIHLIQSLSFGFSQGSFSTMIASVPPQRRALSLLFAGLLAGLGWH
                     LLAKKGKDIQSIQQIIQDDISFSPWTQFWHGWLQLTTVSMGAPVGREGASREVAVTLT
                     SLWSQRCNLSKADQKLLLACASGAALGAVYNAPLATILFILEAILNRWSLKNIYAACL
                     TSYVAVETVALLQGRHEIQYLMPQQHWTLGTLIGSVLAGLILSLFAHAYKHLLKHLPK
                     ADAKSQWFIPKVLIAFSLIAGLSIFFPEILGNGKAGLLFFLHEEPHLSYISWLLVAKA
                     VAISLVFASGAKGGKIAPSMMLGGASGLLLAILSQYLIPLSLSNTLAIMVGATIFLGV
                     INKIPLAAPVFLVEITGQSLLMIIPLALANLIFYFSYQFYRFILK"
     gene            complement(43452..44411)
                     /gene="nrdF"
                     /locus_tag="ACGEUI_000630"
     CDS             complement(43452..44411)
                     /gene="nrdF"
                     /locus_tag="ACGEUI_000630"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFF8165487.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSLQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKKEIEEIFEWTNNNEFLQEKARIINDIYANGDALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMSDDDYNYGL"
     gene            complement(44744..46903)
                     /gene="nrdE"
                     /locus_tag="ACGEUI_000631"
     CDS             complement(44744..46903)
                     /gene="nrdE"
                     /locus_tag="ACGEUI_000631"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFF8165488.1"
                     /translation="MSLKDLGDISYFRLNNEINRPVNGKIPLHKDKEALKAFSAENVL
                     PNTMSFTSITEKIEYLISNDYIESAFIQKYRPEFITELDSIIKSENFRFKSFMAAYKF
                     YQQYALKTNDGEHYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLDITNMYDELVANPKI
                     TKTKIKARDLETEISKLQQESGYPYIINIDTANKANPIDGKIIMSNLCSEILQVQTPS
                     LINDAQEFVEMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSSIEAVPT
                     IKHGNSQAHTFGLGAMGLHSYLAQHHIEYGSPESIEFTDIYFMLLNYWTLVESNNIAR
                     ERQTTFVGFENSKYANGSYFDKYVTGHFVPKSDLVKDLFKDHFIPQASDWEALRDAVQ
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLS
                     ILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI"
     gene            complement(46923..47141)
                     /locus_tag="ACGEUI_000632"
     CDS             complement(46923..47141)
                     /locus_tag="ACGEUI_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HFF8165489.1"
                     /translation="MITVYSKNNCMQCKMTKKFLEQHGANFQEINIDEHPEKVDYVKS
                     LGFTSAPVIEADNLVFSGFQPAKLKELI"
BASE COUNT        14474 a         8299 c         9735 g        14668 t
ORIGIN      
        1 taacttttaa ttgcgaggga actaaaaatg acaatcagac aatggatggc agaccatctt
       61 catttgatgg agactttagc gtgtttggta ttaataataa ttggcctagc ctttttacac
      121 tcatttccac aagtggcttc cgctattttc attacagcct tcttgattgg aggatatgcg
      181 tctgcaaaaa cgggtatatt ggatttggtg aagaacaaac acttgtcagt ggatattttg
      241 atgattttgg cagctatcgg tgctggaatt atcggctatt ggctggaggg tgctctgctt
      301 atttttatct tttcgttgtc caatacgctt gaagaaatgg ccatggaaaa aagtaaggat
      361 gctatttcag ccttgatgtc cttgacgcca gatacagctc gtcaatacca agaagatggc
      421 catattttag aagttgagac cagatccttg agtgttggtg accgtttaca agttcgtaaa
      481 ggcgaagctg ttccaattga cgggcagttg cttagtcctt ttggtcaatt tgatgaatct
      541 atggtcactg gtgagcctat cactgttgat aaggcagaag gccaggatct gattggggga
      601 accattaacc aaggacaaac catagatatg ttggttacta ttgaaaatga cgatactctc
      661 tttgccaaga ttattaatct ggtggaatct gcccaagaaa agaaaagcaa aaccgccacc
      721 tttatcgaaa gcttagaaga tggttatgtc aaatttgtgc tcgtccttat tcctgccttt
      781 atcctcttta gccactttgt gctttcttgg acttggttgg ctgcttttta ccgagggatg
      841 attctcttaa cagtagcttc accatgtgcc ctaattgcca gttccacacc tgctagcttg
      901 gctgctattt ctcgtgcagc cagaaaggga ttgattatca aaggagggga tattgtcgat
      961 aacatgggag atattaaggc tgttgtcatg gataaaacgg gaacgctcac ccaaggaaaa
     1021 ccttctgttg tgaatgctca ttatttggaa gatgagttgc tcgtgaatag actggtaaaa
     1081 ggagcagaga ctgctagtac ccatcctatt tctaaagccc ttcttgaata cactgaaaaa
     1141 ttggagccac tgacctttga ccacttagaa gaaatttctg ggaaaggttt tcagggcttc
     1201 tatcaagggc aagaatggcg aattggcaag aaaacctaca ttttggaaaa ggttcaagac
     1261 ctatcagctt ttgaagaaac tattcaagtg gaagaaaaac aagggaaaac cctaatcttt
     1321 gtttcacgtg accatcaatt gatagcttac tatgccctct tggatgatat caaaatagaa
     1381 tcaaaacgtg ctattaagtc tcttcatgcc atgggaatca aaacagtcat gttaacaggt
     1441 gaccaagaac gaaccgccaa ttatgtggca caaaaacttg gtattgatga agtggtagcc
     1501 aactgtatgc ctcaagataa ggtggctaag ttagcagaat taaagactaa atatggtttt
     1561 gtggctatgg taggagatgg tattaatgat gctcctgccc ttgctcaagc agatgtttct
     1621 tatgctattg gatcaggaac agatattgca atggaaagtg cagacagtgt gattatggat
     1681 gacttgactc gtattccatt ttcgattcaa ctttcccgca caatgaagac cattatcaaa
     1741 caaaatattg tttttgcctt atctgtgatt accttattga ttttagccaa tgtttttcag
     1801 gtagttaact tgccgcttgg tgttgttgga cacgaaggct caacgatttt agtgatttta
     1861 aatggcttgc gtttactttc ttttaaataa tttagaaact tgcttgaaaa cttgaaagtg
     1921 tttataataa tagtaagact tatgaaagag aaaaccgccg tatgtttact attatgatca
     1981 ttggtttagc tgtattttca ataattaaaa tcattctatt tttctatttt tatcagcaat
     2041 ttgagcgcaa gcaaaaaaga aaagttgaat caaacaacca gcaaagacag tcccaagaaa
     2101 gtgacgctgt cccttcaaaa aaattcccat tttaaaaagc ttggaaagtg tacaaagtgc
     2161 acatttccaa gcttttttga ctattaaggt taatctttgt aacgtaagtt cccacgaaaa
     2221 tcgtctaagc tttggtagcc tttttctacc ataatcgttt taagttcctt agtgacccgt
     2281 tcaaagatag ctggcccttc ttgatgaagg gctgtcccaa tttggaccat gctggcgcca
     2341 caaagaatat gttcaaaggc atcgcgtcct gttttaactc cccctgtacc aataatgtgg
     2401 atagaaggtt ttaagcgttt gtaaaacgca tggacattgg ctagagcagt aggtttgatg
     2461 taatctccac ctataccacc aaagccattt ttaggcttga tgaggacttg ttcatcctta
     2521 atgaccagcc catttccgat agaattgaca cagttaacaa aggaaagggg gtatttattg
     2581 aaaatcgcag ccgcttgatc aaaatgcaca atgtcaaaat aaggaggtaa tttgatacca
     2641 agaggtttag tgtaataggt aaagatgttc tctagaagtt gatcagtggt ttcaaaatca
     2701 taagcaatct gtggttttcc tggaacgtta gggcaagaaa ggtttagttc cactagaccc
     2761 tcataatctg aagccatgat ggcttttaaa atggtttcgg tttcagtagg agatagcccg
     2821 acaacggaga gaaagtgagg tttatgttgc ccggttttgg caagatcact gacaaagtct
     2881 aagtagtagc gaaaaccatt attagggagt cccattgaat tgatagaacc aagacgagta
     2941 tctgcgtagc gaggttctgg attacctgga cgaacttcaa gcgtccctgt tttggtgaca
     3001 aaggaagcgg cttgagactt ttcaacctcc ataagctctt cttttgtcat gcagtaaaca
     3061 ccagcagcat tcatcaaaca gttatcaaaa gagaaatgac caatttgtgt agcagtagaa
     3121 accattattt cctccatatt ttagacaaat tttttctatt ctaccactag gcatgaataa
     3181 tagcaagcta tttttacaaa aatgtaaata tattactgtt attgacgaac atttgaaatt
     3241 ttcatgatag tcacattatg attttcaata aagtcagaat agactagctt ttttgctata
     3301 ttttttgtaa aataggaact gtaaaaaggt agcaaccttt aaattaaaag gagatattaa
     3361 ctcatggtaa aattggtttt cgctcgccac ggtgagtcag aatggaacaa agctaacctc
     3421 ttcactggtt gggcagatgt tgatctttca gaaaaaggga cacaacaagc gattgatgca
     3481 ggtaaattga tcaaagaagc aggtattgaa tttgaccttg ctttcacttc agtattgaca
     3541 cgtgctatca aaacaactaa ccttgccctt gaaaatgcag gtcaattgtg ggttccaact
     3601 gaaaaatcat ggcgcttgaa cgagcgtcac tatggtgctt tgactggcaa aaacaaagct
     3661 gaagctgcag aacaatttgg tgatgaacaa gttcatatct ggcgtcgttc atacgacgtg
     3721 ttgccgccag ctatggctaa agatgatgag tattcagcac acaaagaccg tcgttatgct
     3781 gatcttgacc cagcccttat tccagatgct gaaaacttaa aagtaactct tgaacgcgca
     3841 atgccttact gggaagaaaa aattgctcca gctcttcttg acggtaaaaa cgtctttgtt
     3901 ggcgcacatg gtaactcaat ccgcgctctt gtgaaacaca ttaaaggtct ttcagatgac
     3961 gaaatcatgg atgtggaaat tccaaacttc ccaccacttg tttttgaatt agatgaaaaa
     4021 cttaacattg ttaaagaata ctaccttggt ggtgaataac ttaaaaacca gactttggtc
     4081 tggttttttg gttctatatc ctaagaagaa agaaaaccct gcttaaaaat atgatatgat
     4141 agaaataaaa aaggatagat agattatgaa agcagaagat cgtcgtcaaa aaattattga
     4201 gtgcttaaat agtgaacaaa aagctgtatc tgctactcgt ttaggtaagt tattaggagt
     4261 gagtcgccaa gtaatcgttg gagatatagc tcttcttcga gctcagcaga ttgatattat
     4321 ttcgactcca aaaggttata ttatgtcaac agcactttat agtcaccaat tttgtgcacg
     4381 tattgtgtgc caacataatg tagaagaaac taaaaaagaa ttagaaatta ttttagctca
     4441 tcaagggatt attacgacgg tagaggtaga acatcctatt tacggtatga taacagcccc
     4501 tttaaatatt aagacacact ctgatgtaac taatttcatg tccaaactca gtcaatctaa
     4561 agcagagtta ttgtcttcgt tgacagaagg actacatagt cacttaattt cgtgtcctag
     4621 tcaagaagcc tttttagcta ttaagcatga tttagaatta gcaggtatct tatacaaagg
     4681 aatataacta tagtatatta tgccattgat ggttgttatg aataccatgc taaaatattc
     4741 tattgattat ttatcagcaa caaggtatcg tttaaaaata ccaaaagata caggtgtctt
     4801 gatagttatt ggtttttaag gtatactatt tttgaaaagg agataaaatg actaaaaaac
     4861 caacacaact aatagcttat acctctattt tggtcgcatt tgcgattttg attccgataa
     4921 tcatgcctct taagcttatt attggaccag cttcctttac tctggcgagc catgttcctt
     4981 tgtttttggc tatttttatg tcgattccag tagcaatttt ggtcgctctt ggtaccacat
     5041 taggtttttt attagctgga ctacctttaa ttattgtttt aagagcactc tctcatttat
     5101 tgtttgctat tttggcagcg tggtggttaa gtagaaaacc acaactgatg acatcggcag
     5161 taaaatgttt tagttttgcg tttttcatta atgtcattca tggcttggct gaatttttag
     5221 ttgtctatat tttaacagca accacagcaa cttctatgtc ttatttttgg tctatgctag
     5281 gcttgattgg cttaggttcg cttattcatg gcatattgga tttctatctt gctttagttc
     5341 tatggcgttt tttagcaaaa aacttaaaac ttcccaccat aaatgcctaa gactaaaata
     5401 gagatagtta tatgataatg acgagccctt tcttgttgaa agggcttgca ttttttagga
     5461 aaattgatat actaaagtgt gtgtttgtga atacagtaac aaaaggagat atcatgaaaa
     5521 caccagaaca gattttagaa gcaactattc atattgggga acataaggtt accaaaactt
     5581 ttttggctaa atctatctta ggctttattg gtggagcgat gattagcctt ggttatttac
     5641 tatacgtgag aattgccgcc agtggtttag aaacttttgg tgctttttcc agtatcgtag
     5701 gagcttgtgc ttttccgatt ggtctaatta ttattttaat ggcgggtgga gaattaatta
     5761 cagggaacat gatggctgtt tctgcagctt tgttggccaa aaaaattaag tttagcgagc
     5821 ttgccaagaa ttggcttatc attaccttgt ttaatgtgat tggagctgtt tttgtagctt
     5881 ttgtgtttgg tcattttcta ggcttaacat cagctggtat ttttaaagaa gaagtgattg
     5941 aggtggccca tgctaaaata gcagcgagcc ctttgcaggc actagtatca ggaattggtt
     6001 gtaactggtt tgttggtttg gccttgtggc tatgctatgg ggcaaatgat gctgctggta
     6061 aattccttgg tacttggttt cctgtcatga cctttgtagc ccttggtttt cagcatagtg
     6121 tagcaaatgc ttttgtcatt cctgctgcta tttttgaagg aggagcaaca tggttggatt
     6181 ttgtcactaa ttttattttt gtctatagtg gtaatattat cggtggagcc atttttgtta
     6241 gttttttata ttttaaagtt tactatcatc cgcaaaagag taaaactcaa tgagaaaata
     6301 tttttttaga ctgagggcgt cctattctca gtcttctttt tataatttat ggtaaaataa
     6361 gaagcgaagt ttaaaaaatt tagctaaata atggttagtt tattccctaa aaaaggagta
     6421 ttcccaaaaa atccgtaaaa agaatttgcc atgggagtag ttcccaaaat aatgcagatc
     6481 atttagttgt ctttcaaaaa tagccttgat agggtaaaaa gtggacagtg atgcagcaac
     6541 agattcatca ggcgtacaaa ctgtcttatg gtttgtgctt agagatttct taataatgct
     6601 tttggtaata cacttttagt gaagaaagtg tagcatgaaa gcttaaagat aaccatgagt
     6661 tcgctttatg cactattgtt gagaaaaata gcatagtcaa ttgagtaaac atcatttctg
     6721 catggaatga aacaattcct ataaacttat atcataagga tggtttgcgg tttctaaaaa
     6781 agtaacatga atcaaaaaac aaaagccaaa tatcccaagg ccaaggtaac aagtcgagta
     6841 aaaaagtttt tacttggcta atccaaacgg ttctgtcacg attcacgcta aaattggaac
     6901 tgttgagact tgtatcacag acaattcaga tcagacctta gtaacttaca acttaaatgt
     6961 agtagcttat aatacaaata acgaagataa tattgcggtt gctgttatgt atccacatat
     7021 tacgaatata tcggcaaaga tgtgagagcg ctttatgtgg cgatatatgg aaaacaataa
     7081 aggagaactt agtgctttat ccaacaccca ttgcaaagtt aattgacagt tactctaaac
     7141 ttccaggaat tggtatcaag acggcgacga gattagcctt ttatactatt ggaatgtcaa
     7201 atgaagatgt caatgatttt gctaaaaact tattagcagc taaaagagaa ctgacctatt
     7261 gttcgatttg tggaaacctt accgatgacg atccttgtca catttgcaca gacacgagtc
     7321 gtgatcagac gaccattctg gtagtagaag atgctaaaga tgtttctgcc atggaaaaaa
     7381 tccaagagta tcatggctat tatcatgtgc ttcacggctt gatttcgcct atgaatggtg
     7441 tggggccaga tgacatcaac cttaaaagtt taattacccg tctaatggat ggtaaggtga
     7501 gcgaagttat cgtagctacc aatgccacag cagatgggga agcaacgtcc atgtatattt
     7561 cacgtgtctt gaaaccagcc gggattaagg taactcgttt ggcaagaggt ctcgcagtag
     7621 gttcagatat tgagtatgct gatgaagtaa cattattgag agctattgag aatcgtactg
     7681 aactttaatg atcatgttgg tgcctttact cgcaaagaga ggttttgaaa aggctacatc
     7741 tttttgttat aataaaaaaa cattcaattc agataagaaa ggttagctat gtccaaacaa
     7801 acgttagttc tattatacgg aggccgctca gcagaacgtg aagtctcagt gctttcagca
     7861 gagagtgtga tgcgagcagt caactatgat aagttcttag taaaaactta ttttattact
     7921 cagatgggcc aatttatcaa aactcagcaa ttttcagaaa agccatcgga atcagaacgc
     7981 ttgatgacaa atgagacgat agagctaact caaaaaataa aaccaagtga tatctatgaa
     8041 gaaggtgcag ttgtatttcc tgtattacat ggacctatgg gagaagatgg ctctatccaa
     8101 ggtttcttag aagtgcttag aatgccatat atagggacta atgtcatgtc ttctagcata
     8161 gctatggata aaattactac taaacgtgtc ttagaatcaa ttggaattcc acaagttgcc
     8221 tatactgttt atattgacgg acaagatttg gaggcttgtt tagttgaaac tttagcaaga
     8281 cttacttttc ctatcttcgt aaaaccagct aatatgggtt cttctgtggg gatttccaaa
     8341 gcccaaacaa aagtagaatt aagaaaagcg attcagttag ccctgaccta tgacagtcgt
     8401 gtcttgattg aacaaggcgt ggttgcaaga gaaattgaag ttggtctcct tggaaatgat
     8461 aaggttaaat caactctacc aggagaagta atcaaggacg ttgattttta cgactaccag
     8521 gcaaaatatg ttgacaataa gattacgatg gccattccag cagatgtaga ccagagtatt
     8581 gtgacagaaa tgcgttctta tgctgaagta gcctttaaag ccttaggtgg ttgtgggttg
     8641 tcccgttgtg actttttctt gacccaagat ggacaggttt accttaatga attaaacacc
     8701 atgccaggct ttacccaatg gtctatgtat ccactcttat gggaaaatat gggattggct
     8761 tatccagatt tgattgaaga gctggtgaca ctggcacaag aaatgtttga ccagcgtgag
     8821 agccacctga tttaaaccta tgagatgaat aaacattgac ttggatggaa cggcaaacat
     8881 cgtcaagatg tgacgttcgt cctttttttg ttgctcttta tagtagagtc attaagggag
     8941 tttgacattt tagaaatttt tctaatgttt tgtgctataa tagataggac atcgtaaata
     9001 ctagtgttct tagaaagaaa acaaaatgaa attaacctta cacgaagtgg ctaaaatagt
     9061 tgacgcccaa aataatgtgt ctgacttaga cgacgttcct ctgcatcaca ttgaatttga
     9121 tagccgaaag attacaaaag gagacctctt tttaccctta aaaggtcaaa gggatggtca
     9181 tgagtttatt gatttagcct ttcaaaatgg agctgtcgct actttttcag aaaaagaatt
     9241 gccggggaaa cctcaccttc ttgttgaaga ctgcctaaaa gcttttcaaa agctagccca
     9301 ttattatatt gataagatgc gcgtggatgt tattgctgtg acaggctcaa atggtaaaac
     9361 ttccaccaaa gatatgatag gagctgtcct atcaaccact tacaaaactt acaaaacgca
     9421 aggaaattac aataatgaga ttggtttgcc ttacaccgtt ctccacatgc ctgacgatac
     9481 ggaaaaaatt gttcttgaaa tgggacaaga tcacatggga gatattcgtc ttctgtctga
     9541 aattgcaagg cctcgtattg cagttttaac cttagtcggc gaagctcatt tagaatattt
     9601 tggaagccga gacaaaatag cccaaggcaa aatgcagatt gttgacggca tgaattcaga
     9661 tgggatttta attgctccgg gagatccgat tattgaccca tatcttccag aaaatcagat
     9721 ggttatccgt tttggaaatc agcaggaaat tgacgttact ggtattcaag aagacaaaga
     9781 tagcttaacg tttacaacaa atgtgttagc aacacctgtg tccctacctc taccaggaaa
     9841 atacaacgcg accaatgcta tggtagcagc ctatgtagga aaactattgg cagtaacaga
     9901 cgaggatatt atagctgctt tacagactgt gacgttgaca ggtaatcgta cggagtggaa
     9961 aaaagcagcc aatggtgctg atattttgtc agatgtctac aatgccaacc caacagccat
    10021 gcgtctgatt ttagaaacat ttgctaacat agcaaagaac cccggaggaa aaaagattgc
    10081 agtgctagca gatatgaaag aacttggaaa ggattcagtg atacttcatt ctcagttaat
    10141 tgacagctta acatcaggta atattgatca gcttgttttt tatggggatc atattaaaga
    10201 attagcacgg ttggcaagtc aggtttatcc tgctgagcaa gtacattatt ttttaaaaac
    10261 tgaacaagaa gaccagtttg aagccatggc acaatatgtt caaaacattc ttaatccctt
    10321 tgatcaaatt cttttaaaag gcagccactc gatgtcgcta gaaaagttag tagatcgatt
    10381 agtgactgac aagaattgtg acagctatca gccttgattt ttataaaact ttcagagaaa
    10441 ggcttgttct ctcatgaaga ttaaggtata atgaaaaaat atagaaaggg acgattatgg
    10501 atttttttgc aattgacccc atcggactgc ctcatacctc acttatcttt tacttgtcgt
    10561 cactcttgat agcgcttttg tttgtttttt tgacttttca agcatatagg ttaaaatctt
    10621 atcgttattt tttcttgttt ttacagttat ctcaggtgat tggcttatac acatggtacg
    10681 tgttaagggg gtttccttta gatgaagctt tgccgttgta tcattgtcgc atcgctatgt
    10741 tagctatctt ttttctacct gatcgtaaca agtttaagca attatttatg gttttgggga
    10801 taggtggaac attccttgca cttttatcgc cagatcttta tccgtttaga ttatggcatg
    10861 tagcaaatgt ctcgttttat tttggtcatt atgctttgtt agtgaatggt ttgatttacc
    10921 tcttgcgttt ttacgatgcc agtcagttaa gactgctatc agtagtacgc tatttagcaa
    10981 ctgttaactt tcttcttctg ttggtcagtt tagccacgaa aggaaattat ggttttgtga
    11041 tggatattcc agtgattcat acgcgtcatt tgctacttaa ttttgtgatc gttacaagtg
    11101 gcttgacttt tatggttaaa ataaccgaat atttttacct taaatttggt gaagctcaac
    11161 agctggcact tgctttctct aaagaaaagt aggtcgttga aaaagcactt aaaatcagct
    11221 ataataagaa gagtctggga tgatttccag gctcgatttt taaaaaaaag acactcattg
    11281 atagcccaag ataagttata ataccagttt tgaagaactt tagaggctaa tcgtatatgg
    11341 agaaacccaa tgtagcccaa ctcttttgat aaacaagtaa tacctaacga aaataactca
    11401 gagaaaagct ctgccatttt actatacaag gaaaaaagaa tgtcacttac agaagaaatt
    11461 aaaaaacgcc gtacttttgc catcatttct cacccggatg ctggtaagac gactattact
    11521 gagcaactat tatattttgg tggtgaaatt agagaagcag ggacagtaaa aggcaaaaaa
    11581 tcaggtacgt ttgcaaagtc cgactggatg gatattgaaa agcaacgtgg tatctctgtt
    11641 acttcatctg ttatgcaatt tgattacgcg ggtaaacgtg ttaatatttt ggatacacct
    11701 ggacatgagg atttctcgga agatacatac cgtaccttaa tggctgtgga tgcggctgtc
    11761 atggttgtag attcggctaa aggtattgag gcgcaaacga aaaaactttt tgaagttgtc
    11821 aaacatcgta atattcctgt cttcaccttt attaacaagt tagaccgtga tgggcgtgag
    11881 cctttagaac tcttggaaga attagaagaa gttttaggga ttgcttctta tccgatgaac
    11941 tggccgattg gtatgggacg cgcctttgag ggcttatatg atttacataa taaacgttta
    12001 gaactctata agggagacga gcgttttgca agtattgaag acggtgatca gctgtttgcc
    12061 aataatccct tttatgaaca agtcaaagaa gatattgaac ttcttcaaga agcaggtaat
    12121 gacttttcag aacaagctat tcttgatggt gacctaactc cagtcttttt tggctctgcc
    12181 cttacgaact ttggagttca gacttttctt gatactttct tggagtttgc cccagaacct
    12241 catggtcaca agactacaga aggtaatgtt gttgacccct tagctaaaga tttttcaggt
    12301 tttgtcttta aaattcaagc taacatggac cctaaacacc gtgaccgtat tgcttttgtg
    12361 cgtattgttt ctggtgaatt tgagcgcggt atgggagtta atttgacacg cactggcaaa
    12421 ggcgctaagc tctccaatgt tactcaattt atggcagaat cacgggaaaa tgtgacgaat
    12481 gctgttgctg gggatattat cggggtttat gatacaggaa cttatcaagt tggggatacc
    12541 ttaacagttg ggaaaaataa atttgaattt gaacccttac caacttttac cccagaaatc
    12601 ttcatgaagg tttctccgaa aaatgttatg aagcagaaat ccttccacaa aggtattgaa
    12661 caattagtac aagaaggagc aattcagctt tataagaatt atcagacagg cgaatacatg
    12721 ctgggtgctg tgggtcaact gcagtttgaa gtctttaaac atcgtatgga aggtgaatac
    12781 aacgctgaag ttgtgatgac accaatgggt aaaaagactg ttcgctggat ttcagaagat
    12841 gatttggatc aacgcatgtc ttcaagccga aacattttag ctaaggatcg ttttgaccaa
    12901 ccagttttcc tatttgaaaa tgattttgcc cttcgctggt ttgcagacaa atacccagat
    12961 gtcactttag aagagaaaat gtaattgaaa catgattttc aacagtctaa cgttttggag
    13021 atcaatttac tgagcaactt tttaaaagaa atgagtggct agttatctct tttgcagatt
    13081 taagattcta tcaaaaaacg atatcttcac aactgtaagc atatttatga tttagcggat
    13141 aatcgtgaaa gcgtgttatt gcttatatta gccttatttc ctatcttttt gattctaatt
    13201 gtgttacact agttaggttg ttaatcaaac gacgaaaatt aaaaggttta taaggcagct
    13261 tcgcactgcc tttttagaaa agagaaacaa ttgaaattta cagaatttaa cttatcacaa
    13321 gacatccaat cagctgttgt taccgctggt tttgagaaag catctcctat ccaggaaatg
    13381 acgattcctc tcgcactaga agggaaggat gttattggtc aagcacaaac aggaactggt
    13441 aaaacagcag cgtttggtct accaaccttg aataagattc gtactaacga aaatatcatc
    13501 caagcattgg tcattgcacc aacgcgtgaa ttggctgtac aaagtcagga agaattgttc
    13561 cgttttggac gtgaaaaagg ggttaaggtt cgctctgttt acggtggatc aagtattgaa
    13621 aaacaaatca aagcccttaa atcaggtgcc cacattgtcg tgggaacacc gggtcgcttg
    13681 ttagatttga ttaagcgcaa ggctcttatt ttagatcatg tggaaacctt aatccttgat
    13741 gaagcagatg aaatgcttaa catgggattc ttggaagaca ttgaagctat tattagtcgt
    13801 gtcccagcag accgtcaaac cttgctcttc tccgcaacga tgccggcacc tattaaacaa
    13861 atcggggtta aattcatgaa agaccctgaa catgttcaaa ttaagaacaa agaattaaca
    13921 aacgtcaatg ttgatcaata ctatgttcgt gtgaaagaac aagaaaaatt tgatactatg
    13981 acccgtctga tggatgttaa ccagccagaa ttatccatcg tttttgggcg gactaagcgc
    14041 cgagttgatg aaattacacg tggcttgaaa cttcgtggtt tccgtgccga aggaatccat
    14101 ggtgatcttg accaaaataa acgcttacgt gttatccgtg atttcaaaaa cgaccaaatt
    14161 gatattcttg ttgccactga tgtagcagcg cgtggccttg atatttcagg agttacccac
    14221 gtttataact acgatattac ccaagatcca gaaagttatg ttcaccgtat tggtcgtaca
    14281 ggtcgtgccg gtaaatctgg tgagtctatc acttttgtct caccaaatga gatgggctac
    14341 ctctcaatga ttgagaactt aacgaaaaaa cagatgaaac ctcttcgtcc agcaactgct
    14401 gaagaggcct ttcaagccaa gaaaaaagta gccctcaaga aaattgaacg cgactttgcg
    14461 gatgagacta ttcgttcaaa ctttgataaa ttcaaaggcg atgctgtcca attggctgcc
    14521 gaattcactc ctgaagaatt agctttatat attttgagct taacggttca agatccagat
    14581 agcttaccgg aagttgaaat tgcacgtgaa aaaccattac cattcaaata tgtgggtggt
    14641 ggtcacggca ataaaaatgg caaaggtggt cgtggacgtg acaaccgtaa ccgtggtgat
    14701 cgccgtggcg gctatcgtgg tgaccgtaac agagatgagc gtgatggtga ccgtcgtcgt
    14761 caaaaacgtg ataagcgtga tggtcacgat ggcagtggca accgcgattt caaacgtaaa
    14821 tcaaaacgta atagtaaaga ctttttcaat aaagagaaaa aatcaagtgc taaaaatact
    14881 ggttttgtga ttcgtcacaa aggtgaataa gcttaaagag agttgatgag agtcagctct
    14941 ttttaatttt ctgaaaaagt tgcttttata atggaatctg ttataatggt ttcagttatt
    15001 tttgtgataa tttttaaaaa attaggagga gtataagatg tctgatagcc atcttactgc
    15061 ctttgacaaa gcttcaaaag caggttttat tattgcttta ggtattgttt atggagatat
    15121 tggaacaagc cccctttata cgatacagtc tctagttgaa aatcaaggag gggttaatca
    15181 ggtttctgaa tcctttattt taggatcaat ctctttaatt atttggacac tgaccttaat
    15241 cacaacaatc aagtacgttt tgattgctct aaaagcggat aaccaccatg aaggtgggat
    15301 tttttcttta ttcaccttgg tacgtaaaat gtcaccgtgg ctcattattc cggcgatgat
    15361 tggaggtgca acacttttgt ctgatggggc tcttacacct gcggtaacag ttacttcagc
    15421 tattgagggc ttgaaagccg ttcctggtct gagtcacatc taccaaaatc aaaccaatgt
    15481 cattatcaca actctagtga ttttaatagt cttgtttggt attcagcggt ttggaacagg
    15541 ttttattggt aaaatttttg gaccagttat gtttatctgg tttagttttc taggtgttag
    15601 tggttttttt aatacgttgg ggcatctgga aattttcaag gctatcaatc cttactatgc
    15661 tctgcatttg ctgtttagtc ctgaaaacca tcgtgggatt tttatcttgg ggtccatttt
    15721 tcttgcgaca acaggagctg aagctcttta ttcggattta ggacatgttg gtcgaggaaa
    15781 tatttacgtg agctggccct ttgttaagat gtgtattgtg ttgtcttact gtggccaggc
    15841 tgcgtggatt ttagcgaata aacactcagg tattgaacta aaccctttct ttgctagtgt
    15901 gccatcacaa ttaagagttt accttgttag tttggcaacc ttggcagcta ttattgcctc
    15961 acaggctctt atttcaggct cctttacctt ggtttcagag gccatgcgtt tgaaaatttt
    16021 ccctttgttt cgtgtgactt acccaggggc aaatttgggg caattgtata ttcctgtcat
    16081 taactggatt ttatttgcag tgacctcttg tactgttttg gcttttagaa catcagccca
    16141 catggaggca gcttatggtc ttgctattac catcaccatg ttgatgacaa caatcctctt
    16201 aaagtattac ttaatcaaga aaggaacgag acccatcttg gctcatttag tgatggcttt
    16261 ctttgccctt gtagaattca ttttcttctt ggcttcagct atcaaattta tgcacggtgg
    16321 ctatgcggtt gttatcttgg cacttgcaat tgtctttgtc atgtttatct ggcatgcggg
    16381 cacacggatt gttttcaagt atgtgaaatc tcttaatttg aatgattaca aggagcagat
    16441 taaacaactt cgtgacgacg tttgttttga cctctaccaa acaaatgttg tctacctatc
    16501 caatcgtatg caggatcata tgattgaccg gtctatttta tactccattt tagacaagcg
    16561 gccaaaacgc gctcaagttt attggtttgt aaatgtccag gtaactgatg aaccttatac
    16621 agctaagtac aaggtagata tgatgggaac ggattatatg gttcgagtca acctttatct
    16681 tggttttaga atgccacaga ctgtgccacg ctatttgcgc acgattgttc aggatttgat
    16741 ggaaagtggc cgtttaccca aacaagaaca ggaatatacc atcacacctg gtcgtgatgt
    16801 tggtgatttt cgctttgttt taatagaaga acgggtctcc aatgcacgtc aattgagtaa
    16861 ctttgaacgc tttattatgc agactaaggc aagcattaaa catgttaccg ctagtccaat
    16921 gcgttggttt gggcttcaat attcagaagt aacccttgaa gtggttccct tgattctatc
    16981 agatgtcttg aagttaccaa tcaaagaatt ggtgcctgtg gaagacagtg aagcataaaa
    17041 aaatcattga gatgataagt ctcaatgatt tttcttttct ttttgatgag tagcaatata
    17101 ggccaatttc tggcttcttg tttttcgttt tttaaagagt gcttcagcgc tcatcgcagc
    17161 ctctttgcta tcaaacactt cgtaataaag taaagaaaca ggtaaccggt agcgagtata
    17221 tttagccccc tttcctgcgt tatgtgtagc taagcgtttt ttcaaatcag ttgtataacc
    17281 agtgtagagg gttttatcaa cacattctaa cacatacata taagcttttt tagtggtcat
    17341 gattaggttt tcctttggaa gcagcaccaa aataaatctc aaaaatatga tcagtgtatt
    17401 ctccgttttc cttgtggaca actaaaggag gtaaaatagt catcccttca atagaaccgt
    17461 ctttgattgc ttcaataagt aacatattag ctgatttacc taatttagga tagacaaatt
    17521 gtaccctttt aggggcaaga ccattagctc taaggctatc tataatttcc aagaatcgat
    17581 ctggtcgatg aaccattgcc agacgaccat ttgatttaag ggcatgacgg gctacctgac
    17641 aaatctcttc taaattagtg gtcacttcat gacgagccag taaataatgt tcagagacgt
    17701 tcttcttaga actttcgtga cttttaaagt aagggggatt gcagagcatc aaatcaactc
    17761 ctgaacgtgg cacatggttt aggaggtttt ttaaatcatc acaaatcatg gttacttggt
    17821 cttccagttg attgagctgg atagagcgtt gtcccatatc agcaagccgt tcttgtaatt
    17881 ctacttcaac gatggctgcc ttggttctag tgctagcaaa taagccaacc gccccatttc
    17941 ctgagcacaa atccacaatt aaccctttgg aaggcatctt ggggaagcgg gataagagga
    18001 cgctatcaat agagtaacta aagacatcct tgttttgaat aatgccaaca tcacttgaaa
    18061 agagttgatc aatacgttcg ccttctttta agattgcttt tatcatacct gttattttaa
    18121 cataaaatag gcttgaggtg aatgtttgaa gagtgatttt gactttcgaa aagtcagctt
    18181 tcgtgataca ataagtaaga ttattctatt aaaggagaag cctgtgtttt acgcttactt
    18241 acgcggttta gtagtctttt tattatgggt tgtcaatggc aatgcccact accatcatga
    18301 agaaaagatg ttagatgctt ctgagaatta tattctggta gcaccccatc gaaccttttg
    18361 ggatccagtt tacatggcat ttgcagcacg tcctaagcag tttattttta tggctaaaaa
    18421 agagttgttt gcaaatcgtc tctttgcttg gtggattaaa atgtgtggtg cttttcctat
    18481 tgatcgtgac aaaccgagtc cagatgctat tcgttaccct gttaatatgc tcaaaaagag
    18541 caatcgttct cttcttatgt ttccaagtgg aagtaggcat tcgcaagagg tcaaaggagg
    18601 ggtcgctgtg attgctaaat tggctaaggt caagattatg ccagcagcct atcaggggcc
    18661 tatgtcggtc aagggattac tagcgggaga acgggttgac atgacttttg ggaatcccat
    18721 tgacgtctca gatattaaac gcatgaatga tgaagggatt gctgaggtgg caaaccgtat
    18781 tcaggcagag tttgatcgga ttgatgacga gttggcacct ttccaaccag gcaaagcaag
    18841 gaacccttta acttatctct accgtttacc attaggattg gttttggtgg tggtattatt
    18901 attaaccatg ttattttcct atattgctag ttttgtttgg aatccagata aacatcgcca
    18961 ttaagaacat gagggctaac atgtgcctga aataagaagt gattgagatt agtcttctat
    19021 tgaaagaagc cgctaatgct taatcacttt tttcatttct aaaaaagata ttcgatgata
    19081 cccttaactc ttttaagcac aaaaaatgac aatactacta tacttttgga tatcatttac
    19141 gaataaaaag atgaggtaaa aaaatgttag acgaattatt aattaaagga aaatcgtttt
    19201 tgagagagcg gtattttctg ccatacctaa ttagtctttt attaggactt tttctaatac
    19261 taagcttcag ttttttgttc tggggaaata ggcagagcaa ggcagccgtt cctgctttga
    19321 gagaaatatc gcctgtgaaa cagcaggtta gtgaagaaaa aaaggaaatt caagaagata
    19381 gcagtatttt agtggattta aaaggcgccg tacaaaaaga gggagtttat aagctaactg
    19441 ctagcagtcg tgtgcgagat gtcattgaac tagctggagg cctaacttca gaagctgata
    19501 aacatgctat taattttgca gaaaaactaa ctgatgagca agttgtttat gtacctaaac
    19561 aaggagaaga gatttctgtg ctaccaagat cattagtttc tggtaaaaaa gaaactgcca
    19621 gcaaggatca gtcaaaggtt catattaata aggccagctt agaagaacta caacatatcc
    19681 caggtattgg tgctaaaaga gctcaggata ttattgatat gagagataag ttaggtggtt
    19741 ttaaagcgtt agaagatctc cgtcaggttt cggggattgg cgaaaaaacg ctcgagaaat
    19801 taaaagatga cattttcttg gactaagcta gtgcccctgt caaagattca atttgctttt
    19861 ttaatcttgg ttttctttta tcaaatccat agccctagct ggttaacgtt tctccttagc
    19921 cttagtttga tttgcttact tgtcaagcgt ttgtctaaaa aagaatttct cggtgtcttt
    19981 gccatcctta gtttctgtgc tttgtttttg ctgtatcaga aacagcagtt ggttcaaaaa
    20041 ctggaaattc agccagtaca aataacctct gtagcattag ttcctgatag tattcggatt
    20101 aatggtgatc aattagctgt tcttggacgt cacgggaaac attcttatca gttattttac
    20161 cgtttgaaaa gccaagcaga agcccaattg ttcaagaaag agcaccgttg gttagtgatg
    20221 cacgcaaaag tgacattgga aaaagctgag gaagttcgaa attttaaagg ctttaattat
    20281 cagacttttt tgacttatca aggaatttac aggataggga aggtagagca aatagaacag
    20341 cttgaagtga tttcacccga gtcaatatgt gactatttgt cctctttaag acgacgagcc
    20401 attgttcatt gccagcagca ttttccaaga cctatgtctc actacctgac gggcctcctt
    20461 tttggttatt tagataagtc gtttggtgag atgacagatt attatagtca gttgggtatc
    20521 attcatctct ttgctttatc agggatgcag gttggcttct ttctcacttg ttttagaagg
    20581 gtgttgctat tactcgcagt tcctttggaa tggataaaat ggatagagct tccttttgct
    20641 tgcttttatg ccgcgttgac aggttatagt atttctgtta ttcgaagcct cgtgcagtcg
    20701 cagttgcgtc atttaggaat aaaaggatta gacaatctgg cttgtacttt tttacttgtc
    20761 tttttatggg atgctcattt cttaatgact gttggtggcg tattaacgtt tagctatgct
    20821 tttttgttaa cagtggttac tgttgaagaa ttatcaggag ctaaaagaca gttggtacaa
    20881 gtcttgacca tttccctagg aatattacca tttttgctct tttatttttc cagttttaac
    20941 ccaatgtcaa tggtcttaac agggctcttg tcttatctct ttgacctttt tattttgccc
    21001 cttctttgtc tggttttttg tctatcccct ttggtgactg tctcaatatg taaccatctt
    21061 tttattttat tagaaaaagt cattcaattt ctcggaaata ctttcaatag ttcccttgtt
    21121 tttggaagcc cgaccagttg gcacttgctt atcttggtga ttagcttcgc tattttttat
    21181 gattatcggc aggtgcgaca acgagttatc acttgcggtt tggttatagc gttgacactt
    21241 ttgtctgtca aatatccttt gaccaatgaa gtgaccttta tcgacattgg ccagggagat
    21301 agtattttgg tgagggagtg gacagggaaa aatctcttaa tagatgtagg tgggcgccct
    21361 ttcttttctt caaaagagca ctggcgacga ggacatcacg tagctaatgc tcagaaaacc
    21421 ttaattcctt acttaaaaag tagaggtatt cacacaattg accaactatt agtaactcac
    21481 gcagatactg atcatatggg ggatatcgaa gtggttgcca aagctattcg gataaaggag
    21541 attttgacta gtcaaggcag tttaagccat ccaagctttg ttaggcggct aaggcgttta
    21601 aaatgtcacg ttagggtttt agcagctgga gaccagttgc ctattatggg aagtgtctta
    21661 caggtactct acccttggca gttaggggac gggaaaaata acgattcttt ggtactttat
    21721 ggcaggctct taaatcgaac ttttcttttc acaggtgact tggaaaaaga gggggaaaac
    21781 gagatcataa aacgttatcc tcaactaaga gtagattatt taaaagcagg tcaccatgga
    21841 tcaaatacct cttctagtgc agctttttta gaccatattc agcctaaagt ggcctttatt
    21901 tctgctggaa aaaataaccg ttatcagcat cctcacagag aaaccttagc ccgtttggaa
    21961 gacagacaaa tcacttatta ccgaacggat acgcaaggag ctattcgttt gactggtcgg
    22021 acaagctggc atcttgaaac ggttcgttag ttaaaattcc agcctgacga agaaacaagc
    22081 agtcgctttc cttttaggag aaagatgata taataaaagc atgattgcga tagaaaagat
    22141 tgaaaaactg agtaaagaaa atttgggtct tataaccctt gtcacaggag atgacattgg
    22201 tcagtatagc cagttgaaat cccgcttaat ggagcagatt gcttttgata aggatgattt
    22261 ggcctattct tactttgata tgtctgaggc cgcttatcag gatgcagaaa tggatctagt
    22321 gagcctaccc ttctttgctg agcagaaggt ggttattttt gaccatttgt tagatatcac
    22381 gaccaataaa aaaagtttct taaaagaaaa agacctaaag gcctttgaag cctatttaga
    22441 aaatccctta gagactactc gactaattat ctttgctcca ggtaaattgg atagtaagag
    22501 acggcttgtt aagcttttga aacgtgatgc ccttgtttta gaagccaacc ctctgaaaga
    22561 agcagagcta agaacttatt ttcaaaaata cagtcatcaa ctgggtttag gtttcgagag
    22621 tggtgccttt gaccaattac ttttgaaatc aaacgatgat tttagtcaaa tcatgaaaaa
    22681 catggccttt ttaaaagcct ataaaaaaac gggaaatatt agcctaactg atattgagca
    22741 agccattcct aaaagtttac aagataatat tttcgatctg actagacttg tcctaggagg
    22801 taaaattgat gcggctagag atttgattca tgatttacgg ttatctggag aagatgacat
    22861 taaattaatc gctatcatgc taggccaatt tcgcttattt ttgcagctga ctattcttgc
    22921 tagagatgta aaaaacgagc aacaactagt gattagttta tcagatattc ttgggcggcg
    22981 ggttaatcct taccaggtca agtatgcgtt aaaggattct aggaccttat ctcttgcctt
    23041 tctaacagga gcggtgaaaa ccttgattga gacagattac cagataaaaa caggacttta
    23101 tgagaagagt tatctagttg atattgctct cttaaaaatc atgactcact ctcaaaaata
    23161 ggcgttggac cgctttccaa gagataatca aattagttga aagctcttat tttttgcgat
    23221 aagatagacg tatcataaag gaaaagagga caggaaaatg gctattattt taccagaact
    23281 tccatacgcg tatgatgctc ttgaaccaca gtttgatgcg gagacaatga ctcttcatca
    23341 tgataaacac catgccactt atgtcgctaa taccaatgcg gctttagaaa aacacccaga
    23401 gatcggagaa aatctggagg aactattggc agatgtcact aagattccag aagatattcg
    23461 tcaaacctta attaataatg gtggcggaca cctaaaccat gcgcttttct gggaactatt
    23521 atcacctgaa aaacaagacg tcactcctga tgttgcacaa gccattgatg atgcttttgg
    23581 gtcatttgac gcctttaaag aacaatttac agcagctgct actggacgtt ttggttcagg
    23641 ttgggcatgg ttagttgtta ataaagaagg acaactcgaa atcacatcaa ccgctaacca
    23701 agatactcca atttcagaag gcaaaaaacc aattctagcg cttgatgtgt gggagcatgc
    23761 ttactacttg aattatcgta atgtacgtcc aaattatatc aaagctttct ttgaaatcgt
    23821 taattggaaa aaagtatcag aactttacca agccgctaaa taagaaaagg gtttccaatc
    23881 tcaaaaagag attggttttt taagtgcttt tgtgaataac agtgtgggga catcattttt
    23941 tagaaaagat aaaaatagtt tgtcttttta atgtaattcc ttgattttta gctaaattta
    24001 gctagaatag aatggatagg agattttagg tgttaagaaa gaagaaaact accattgtag
    24061 ggattatttt aggaatgatg atagtgacct tacttgtcgg ctgtttgact ggtaaaaaga
    24121 aaggctttcc tcctaaagca ggtgagcggc agcaaacggc tgacttaaaa atgagaaaaa
    24181 gattagaaaa actgttcaaa aaccgttacc tgaaaaatct aaagaggcta agcaagctgt
    24241 tatggatgca gatttagcgc aaatggatgt cttagggctc tattacgatg acaaccattt
    24301 gagtttggtc aatacggtca aggcgtattt aaaggaattt ggcattggtg aatcccaaat
    24361 tgccttttcc tacaaagacc tcaattcagg caggactgct gccatgaatg accatcagcc
    24421 aatgattgca ggaagcacct ataagctgcc attaaacatg ctggtggtag ataaggcaaa
    24481 ttatccatga cagagcgttt tgacattaca gaaactgatt atgaatatga tcaggaacat
    24541 catgcctatg ttgctcagtt caatggtgcc atgtctattc cggatatgca agaatattcc
    24601 ttggtgtact cagaaaacac gccagcttat gctctagcag aacgattggg tggcatgaat
    24661 aaggcttacc aattatttga ccgctacggg aaggtatccg gggctattac gaccattgat
    24721 cgtaatggca ataaaatcac caccgcttac tatttgcaag ttttggatta cttgtggcag
    24781 catcaggata aatataagga tatcttgtac tacattggtg aatctttccc tgatttgtat
    24841 tacaagactt atttgcctca tgtaaaggtt tatcaaaaac cgggttatgt cagagaagcc
    24901 ttaaatgtag gtgccattgt ttgcgaagag tctccttatt tgattgctct ttattcatct
    24961 ggtttgggtg gagcaacaca agcatcagag gaagtcaatg gccttggtta tgtgcagctt
    25021 gttcagctac catatgtaat taatgagtgg tatagaggca atctcaacat gatctaatac
    25081 tcagttgaca ttagtctgat agtagacata cttactgatg acagtcacta aaataagttg
    25141 ttgctcatta aaaaagcttg gcctgtttct tcaggtcaag ctttttgaga ttaatgctgt
    25201 gtttttgagg tcttattgtc acctcttgtt tcttgataat ggtgaagcaa ttttttgaat
    25261 tttttaacgg cagcgcgtgt tagctgctta gatgatttgc catttctgct gtaaatggga
    25321 ttgtgcttat ccctttgctg ttctaaatcg ggaactagaa aagttgcatc aggactatta
    25381 attgcttttt caataaaagc cggatcaaaa agcagaatag ggtcaatatc aagcagacgg
    25441 tgcaagtcat cgttttggtt gccataattg aaagaagaac cattgacaat actatttcgc
    25501 gttaggttat caggatctag tgctactaaa aattgtccag attctgacaa aataaactct
    25561 gagtgaaaat gaattaaaat cttatagttg gcttgaggaa tattgtctgt atagactgcc
    25621 tgatgattgc cattagcatc taaaatacta gcaaccttat tatggtatct agaagattca
    25681 taaagagcat aatcgtcatc ttctagagta gccaaatatt tggctaaggc atccgcatcg
    25741 gttttccctt tattcccata atgtgagcga acccactccg tttgctgggt tgatatgagg
    25801 taacggagtt gatgaatttt ccgattaaaa cggctataag gaatgtgttc gagattaaca
    25861 taataagcct gaatcagggt agataattgg tcccaaaaag gatctgttgg cgatagcttt
    25921 ggacttaatt gtgccaatac ttgctgtgtt tgttttgatt gtcctgtcaa ctcgtctggc
    25981 atctttaaat acgtcaagag cagttgtaac cgtttttgag cttcttgagg cgtctgtgcc
    26041 atctcctctt gttgccagag cttttgaaag tcaggtgatc ctatcaggcg aatgcgacta
    26101 taaagttgtt cgaaatgata agagtttcct ctaacagcga gttgttcaag gtatcctttt
    26161 tggatggctt tataggacca tccgtcctta tcgagctttc gaacataatc aagcaagtcg
    26221 tggcgtaatt taatagcttc tgaaagctgg agttctcgta atttaggata gttttggtaa
    26281 aaaggtgcca ctaaatcttg ataaagtttg tgttcttttt caaaggcaat ccgctgatag
    26341 tctttctgcc aaaaactgac gcaaaaagtc agaaacatca agaagaatag ccattttcca
    26401 agtaatttca taggttttta gttgacaaac atggcatcgc caaagctaaa gaaacgatac
    26461 ttttcgtcaa ccgcgtgtct gtaagcttca aggacaaagt cacgccctgc aaaggctgat
    26521 actagcatca caagggttga ttttggaaga tggaaattag ttgaaaaagc atcaactacc
    26581 ttgaattggt agcctggttt aataaaaata ttggtccaac ctgaatcagc ttgaatgtcc
    26641 ccttggaact tacctccgat ggtttccaac gtacgaatag aggtggttcc tacagcgaca
    26701 acacgaccgc cagcttgttt gacgtctctt agggtttgcg ctgcttcttc tgagagggaa
    26761 taaaattccg aatgcatatc atgctcatcg aggttatcca cggaaacagg tctaaaagtc
    26821 ccaagcccta catgtagggt caaataaacc aaatgtaccc ctttggcttc gatttttttg
    26881 agcaagtctg ttgtgaagtg gagaccagca gttggggcgg ccgcagagcc attttcttta
    26941 gcataaacgg tttggtatcg ctcagcatcc tctaattttt cgtgaatata aggaggcaat
    27001 ggcatttcgc cgaggctctc taaaacttct aagaaaatcc catcatagct gaattcaaca
    27061 attcgtccac catgttctaa ttcgtcgata atggtggctt tgagacgccc atctccaaaa
    27121 ttgacttggc taccaacctt gagccgtttg gctggcttgg ctaaaacttc ccattgatct
    27181 ccttgtgtat ttttaagcaa aagcaattcc acatgaccat gagtatcggg tttttctcca
    27241 taaagtctgg caggcaagac acgggtgtta ttcatgacga gggcatcccc tggatttaat
    27301 tgatcaatga tatggtcaaa atggctatca accatagttt tttgacggtg atcaataatc
    27361 aatagttttg aactgtctct tttttcgagt ggggtttggg caatcaattc ttctggtaac
    27421 tcaaaatcaa aattgttagt attcattggg actcctttaa agccactagt aaatctcttc
    27481 ctagtaactc tttcatgtaa gataaatctt ttctattata tcacttgtcg tctaacccgt
    27541 catctttaat ctttcatcag aaagtaaagg ataggcttga caaaaattgg tatataccat
    27601 ataataaaaa cacgaaaggt ctataccaat tagaaaagga gaatgacatg aaaattattc
    27661 gggtccaaga tcagattgaa ggtgggaaaa tcgcttttac cttattgaaa gatagcttag
    27721 ctaagggagc taagacgtta ggattagcaa caggaagttc accaattagt ttttaccaag
    27781 aaatggtcaa aagtcctttg gatttttcgg acttaacaag tattaacttg gatgaatatg
    27841 tcggtctttc agtagagagc gatcagagtt atgattattt tatgcgtcaa aaccttttta
    27901 atgcgaagcc ttttaaaaag aattacttgc ccaatggttt agcaaccgat gtcgaagcag
    27961 aagctaaacg atataaccaa atcattgcag agcatccgat tgattttcaa gtgcttggga
    28021 ttgggcgtaa tgggcacatt ggttttaacg aacctgggac ctcttttgaa gaagaaacgc
    28081 atgtggtgga tcttcaagaa tcaaccattg aagccaatag tcgtttcttt acaagtattg
    28141 aggatgtgcc aaaacaagct atctctatgg gaattgcctc catcatgaag tccgaaatga
    28201 ttgttcttct tgcctttgga caagaaaaag ctgatgcgat caagggaatg gtttttggac
    28261 caatcacaga acacctccca gcaagtattc tccaaaaaca tgatcatgtt atcgtcattg
    28321 tggatgaagc tgctgcgtca caattagact aacctaacca gtcgttaaac cttgtaagaa
    28381 caaaaatgag gagatagtca acttcattta agaagaagtc ttgtaacttc tatttctttt
    28441 gcaagggtaa atttatgtta cactactaat atgcgtttag ataaattatt agaaggtaca
    28501 aaagttggct ctagaagtca agtgaagaaa ctgattaagg ctcaaggggt ttgggttgat
    28561 cacatgcctg ccagaaatgg tcgacaaaat gttgatcctg gcttgcaact gattgaggtg
    28621 actggacagc gagtgacgca tcctaaacat tcttacatta tcctcaacaa gccatcaggg
    28681 gtggtctcag ctaagaaaga tactaactat ttaaccgtta ttgatcagct tgctgaagaa
    28741 gataaaagtc cagacttgta tcctgttggc agattggata gagataccga agggttggtt
    28801 ttactcactg ataatggtcc cctagggttt cggatgttgc accctagtca tcatgtttct
    28861 aaaacgtatc ttgtaaccgt taatggcttg ttagcagaag atgcctcaga tttttttgca
    28921 gctggtattt gtttcccaac aggtgaacaa tgccaaccag ctcaattgac tattcttaaa
    28981 gccgatacag accagtcaca ggctagtttg accatttcag aaggaaaatt tcaccaagtg
    29041 aaaaaaatgt ttttgaccta tggtttgaaa gtgacctctc tgaaacggac gcactttgca
    29101 ggactggaac tcggtcattt agccagcgga gaatatcgtt atctaacagc gtcagaaagg
    29161 caactcatta aaacgtatct ggactgagca aaacatgctt tcaaaaagaa gctattcttg
    29221 cttaatcaat aaagtgtcca ttgactatta tagtcatgat gagtccgact tttgctatgt
    29281 ttagtcatct tgttgttaca aaatcaggta agcaaaaaga ttcaattccc actgtctcct
    29341 taacctgaag cgttataaaa acccttgtgt taaactctca taaattggaa atgggagtgt
    29401 tttttgatgg ctaaaaaaac taaagctact taaagtttgg tacttagttt tcgaataata
    29461 aggtggaaga tctaatagga ggtgacaaaa atattaaccg ctttagatga taagaatcag
    29521 ctcatttcgc tagtaactca gcctatttca acgaaaccgc cttttcgttg cccagcttgc
    29581 aaatctcctg ttcgcttgcg tcaggggaca attagacgac ctcattttgc ccatgtacaa
    29641 ctagctcatt gtcaattcca agcagaaaat gaatctgagg aacatttgac gttgaaggca
    29701 aaactttaca ccagtttagt ccgaacagaa gctgtctgta ttgaaaaata tcttcctgag
    29761 ctgcaacaga tagcagacct ttgggtgaat gataagttag ccttagaaat tcagtgcagt
    29821 cccttgccag ttgagcgtct aaagaaacgc accaaagcct atcaagaaaa ggggtatccg
    29881 gtacgatggt tactgggaag aaaattgtgg cttaacactc acttaactgc tttgcaaaaa
    29941 cagtttcttt acttttcatc tagtttgggc tttcatttgt gggagctgga tgcagctgca
    30001 aatctgctac gtctcaaata tttaatccat gaagacttat ttggtaaggt aagttaccta
    30061 accaaaacca tttctttaga ccacaatatt atggaaatgt ttcggttacc ctatcagcaa
    30121 gaaatccttt attcttatca gaaaaaaatg actgtgaacc tatctaaaag aatacaaaga
    30181 gctcttttag ctagacaccc gaaatggcta cgacgacaag aaaaggctta cttatcaggc
    30241 tataatttac ttatgttaac aactgatgct ttttatcctc aatggagacc agttcaatct
    30301 tccagcggtt tttgtcaaat taaaggaaat cttcgccctt attatgaaag ttttaaagtc
    30361 tattataaaa aagaaaagga taagaaggtg caaacgcttt tttcaccaaa atattatgtt
    30421 aaaatggata gtaatagaaa atgagaagga gatgttatga ctgataatcg cagccattta
    30481 gaagaaaaat acacttggga tttgagcact atttttgcaa cagataagga ttgggaagct
    30541 gaagtcagtg acttagctac tgaagttgag gcatctaaag gttttgctgg tcatttgctg
    30601 gattctagtg ctaatttgtt aaaggttaca aaaacatatt tagaattggc tcgtcgtgtg
    30661 gagaaagtct atgtttatgc tcacatgaaa aatgaccaag acactactgt tgccaagtat
    30721 caagaatacc aagctaaggc ttcaggcttg tatgcaaagt ttagcgaagt cttttcattc
    30781 tatgatccag aagtgatgat gcttcatcaa gaagactatc aggcttttct tactgaaacc
    30841 cctgagttga aagtgtacaa ccatttcttt gacaaattat tccaagcccg cgaacatgtt
    30901 cttagtcaag cagaagagga attattagca ggagctcaag aaattttcaa tggtgcggaa
    30961 gaaaccttta gtattcttga taacgctgac attgtctttc cagtagttaa aaatgataag
    31021 ggggaagatg ttgaattaac ccatggtaac ttcattagcc taatggaatc taaggaccgt
    31081 agcgttcgcc aagctgctta tgaagccatg tatagtactt atgaacagtt ccaacatact
    31141 tacgctaaaa ccttgcaaac caatgtgaaa gtgcaaaact ataaggcgcg tgttcataaa
    31201 tacgattcag cacgtcaagc tgccatggct gctaacttta ttccagaagc tgtttacgac
    31261 actttattag aaacggttaa taaacatttg ccactcttac accgttattt gaaattgcgt
    31321 caagaggttc ttggtttaga cgatttgaag atgtatgatg tttacacacc attatctgaa
    31381 acagatctag ccattggtta tgatgaggct ttggaaaagg ctgagaaggt tttggctgtt
    31441 tttggtaaag attacgcaga tcgtgtgcat cgtgctttta ccgaacgttg gattgatgtt
    31501 catgtcaata aagggaagcg ctctggggct tattcaggtg gttcctatga taccaatgcc
    31561 tttattctgt tgaattggca agatacactt gataatcttt acacacttgt ccacgaaaca
    31621 ggccacagtt tgcattctac tttcactcgt gaaacacagc cttatgtgta tggggattat
    31681 agtatcttct tggcagaaat tgcatccaca acgaatgaaa atattatgac agaagctctc
    31741 ttaaatgagg ttcaagatga gaaagaacgc tttgccattt taaaccatta cctcgatggt
    31801 ttccgtggaa cagttttccg tcaaacccaa tttgctgagt ttgaacatgc cattcatcaa
    31861 gctgatcaaa aaggagaagt gttaacaagt gaatacctta accagctata cgctgattta
    31921 aatgaaaaat attatggttt aagtaaaaaa gataatcatt ttattcagta tgaatgggca
    31981 cgtattcctc atttctatta caattattat gtttaccaat atgcaacagg ttttgcagct
    32041 gcaagctatt tagcagataa gattgtgcat ggcacacaag atgatatcga tcattacttg
    32101 gcctacctca agtcaggaaa ttctgattac ccacttgaag tgattgctaa agcaggcgtt
    32161 gatatggaaa aaggtgatta tttggaagcc gcctttaaag tctttgacga acgtctaaca
    32221 gaacttgaag tactcgtgtc caaaggcata cacaaaagct aaaatcacaa aagtcaagga
    32281 caaaaagagt ttaggattga tagaaatgga gattcacagt tggtgtgaat ctccatttct
    32341 atcggtcatt ttctttttat attttagtgg attatcagtc ctgaaaagca ggcttcattg
    32401 accttagttt gacggtttct tttttgtctt ttttattgtg tggtcactca cttaaataaa
    32461 accaatagtt gagtaaggcg cttcagatta acgctattct attgttttag agataacgtt
    32521 cgcataaaac atatttttat tatatttgac attttataaa agagataata gaattaaaat
    32581 actaacattt atgtttttta aaaaataaac gttcgtataa taaaaaagga gtttttcatg
    32641 gaaaaaacaa aacgttatat cattgcaaca gcagggatac ttctccactt aatgttggga
    32701 tcgacctatg cttggagtgt ttatcgtaat ccaattttac aagagaccgg ttgggaccaa
    32761 gctcctgtcg cttttgcctt ttctttagct attttttgtt taggtttatc tgctgctttt
    32821 atgggaaatt tagttgaaca atatggtcct cgtctaaccg gaacggtgtc agctattctt
    32881 tatgcttctg gaaatatgct gactggttta gcgattgatc gtaaagaaat ttggctttta
    32941 tatattggtt atggtgttat tggtggctta ggacttggag cgggatatat cacaccgata
    33001 tcaactatta taaaatggtt tccagataaa agaggcatgg caacagggtt tgctattatg
    33061 gggtttggtt ttgcgtcttt attaacaagt ccgattgccc agtggctaat tgaaacagaa
    33121 ggtttggtag caacatttta ccttttaggg ctgatttatc taatagttat gctttttgct
    33181 tctcaattga taataaaacc aactgctgct gagatagcta tactagataa aaaaagatta
    33241 caaaataaca gttatttaat cgaaggaatg accgctaaag aagcacttaa aacaaaatca
    33301 ttttattgtc tttgggtaat tttatttatc aatatcacat gtggcttagg acttatctca
    33361 gttgttgcgc caatggcaca agatctaact ggtatgagtc cagaaatgtc agcaattgtg
    33421 gttggtgcta tggggatttt taacggtttc ggtcgtttag tgtgggctag tttgtcagac
    33481 tatattggac gccgtgtgac agtaatactt ttatttttag taagcataat tatgactatt
    33541 agtttaatat ttgcacatag ttccctaatt ttcatgataa gcattgccac ccttatgact
    33601 tgttatggtg cgggtttttc tctcattccg ccttatttaa gtgatctttt tggggcaaag
    33661 gaactggcta cattacatgg gtatatattg acagcttggg caatagctgc attaactgga
    33721 cctatgttat tatctatcac tgttgaatgg actcataatt atcttcttac cttatgtgtt
    33781 tttattgtct tatacatttt aggattaatg gtagcattaa ggttaaagaa atgatgtcct
    33841 ataaaaaatt taaaagtaaa ccctagcaaa gcctagggtt tttttatata attaggttta
    33901 tggttaaatc atacagtaaa acagcaaatc ataatatgcg tcgtcctgtt gtcaaagaag
    33961 agcttgttca ttatatgcga acacggcaga agcaaaccac aggtttttta gcagagttag
    34021 agcaattcgc aagacaagaa aacattccta ttattcagcc tgaggtcgtg gcttatttca
    34081 ggtttttatt gcaaagctta cagccaaaac acattttgga gattggcaca gccattggtt
    34141 tttctgcgct tttaatggct gaaaatgcac cagatgcgac gattgtgact attgatcgta
    34201 atcgagagat gatcgatttt gctaaagcaa attttgctaa gtacgacagt cgccaacaga
    34261 tccgcctgtt agaaggagat gcagcagata tattatctac acttgaagga aattttgact
    34321 ttgtgtttat ggattccgcc aaatccaaat atattgtctt tctaccagag attttaagac
    34381 tgctaaaagt cggtggtgta gtcattttgg atgatgtctt tcaaggaggt gacattacaa
    34441 aaccgatcga agatattcga cgaggtcagc gtactattta tcgtggctta cagtctttgt
    34501 ttgatgccac cttaactcat ccaaacctaa caactagcct ggtccctctt agtgatggat
    34561 tattaatgat tcgtaaaaat caagcagata ttgtattacc agattaagta acattgataa
    34621 atttatgtta taataaatta gttaaagata aaaaggagct cagaagtaaa tgaaaaactc
    34681 aaataaactc attgctagtg ttgtgacatt ggcctcagtg atggctttag cagcttgtca
    34741 atcaactaat gacaatacta aggttatttc gatgaaaggt gatacaatta gcgttagtga
    34801 tttttacaat gaaacaaaaa acacagaagt atcgcaaaaa gcgatgctaa atctggtaat
    34861 tagtcgtgtt tttgaagctc aatatggtga taaggtttca aaaaaagaag ttgaaaaggc
    34921 gtatcataaa acagctgaac agtatggcgc ttcattctct gctgctttgg cacaatcaag
    34981 cttgacacct gagactttta agcgtcagat ccgctcttca aaattagtag aatatgcggt
    35041 taaagaagca gctaaaaaag aattgacaac acaagaatat aagaaagcat atgaatctta
    35101 tactccaaca atggcagtcg aaatgattac tttagataat gaagagacag ctaaatcagt
    35161 cttagaggaa ctaaaagccg aaggcgcaga ctttacagct attgctaaag aaaaaacaac
    35221 aacacctgag aaaaaagtga cctataaatt tgattcaggt gcgacaaatg taccgactga
    35281 tgtcgtaaaa gcggcttcaa gtttgaatga gggtggcata tcagacgtta tctcggtttt
    35341 agatccaact tcttatcaaa agaagtttta cattgttaag gtgactaaaa aagcagaaaa
    35401 aaaatcagat tggcaagaat ataagaaacg tttgaaagct atcattatag ctgaaaaatc
    35461 aaaagatatg aatttccaaa acaaggttat tgcaaatgca ttggataaag ctaatgtaaa
    35521 aattaaagac aaagcttttg ctaatatttt ggcgcaatat gcaaatcttg gtcaaaaaac
    35581 taaagctgca agtgaaagtt caacaaccag cgaatcatca aaagctgcag aagagaaccc
    35641 atcagaatca gagcaaacac agacatcatc agctgaagaa ccaactgaga ctgaggctca
    35701 gacgcaagag ccagctgcac aataagtagg attttattga ctaatcagtt aaaaaaactt
    35761 ataataattt agttaagaat tgtttatcaa ggattagata cagaaaagtg acggttgctg
    35821 cgagtcattg ataattagat aaacatgcta cttaacttta caacaattga tatcgtaaat
    35881 taagaatgac aagttcattg aatagaggtg gtaccgcggt tttcgccctc tgtgagatgg
    35941 acttgttttg tattgaggac tatttgaaat caacgaaagg aatagagtag actaggctgc
    36001 caggcttata gctgatctcc tagcaagaaa gaagagctgt gtcaactggt gtgattacat
    36061 tactagcttc agcataacac tgtttttctt actctttgta tctaaatttt tatgaaagaa
    36121 ttatcgtctg cacaaatccg ccaaatgtgg ttggatttct ggaaatctaa aggacattgc
    36181 gttgagcctt cagctaactt ggttcctgtg aacgacccaa cgcttctttg gatcaactca
    36241 ggtgttgcaa ccttgaaaaa atattttgat ggttcagtga ttccagaaaa tccacgtatt
    36301 accaatgcac aaaaatcaat tcgtactaat gatattgaaa atgttggtaa aacagcacgt
    36361 caccatacta tgtttgaaat gcttggtaac ttctcaattg gagactattt ccgtgatgaa
    36421 gctattgagt ggggatttga actcttgaca agtccagact ggtttgattt ccctaaagac
    36481 aagctctaca tgacttatta cccagatgac aaggattcgt ataaccgttg gattgcttgt
    36541 ggcgttgaac caagtcactt ggtgccgatc gaggataact tctgggaaat cggtgctggt
    36601 ccttcaggtc cagatacgga gattttcttc gaccgtggtg aagatttcga tccagaaaat
    36661 atcggacttc gcctcttggc tgaagatatc gaaaacgatc gttacatcga aatctggaac
    36721 atcgttctct cacaattcaa tgctgaccca gccgtaccac gttcagaata caaagaatta
    36781 ccaaacaaaa acattgatac aggtgctggt cttgaacgtc ttgcagctgt tatgcaaggg
    36841 gcaaaaacaa actttgaaac tgacctcttc atgccaatca tccgtgaagt agagaagttg
    36901 tcaggtaaaa cttacgatcc agatggcgac aacatgagtt tcaaggttat cgctgaccac
    36961 atccgtgcgc tttcatttgc tatcggtgat ggtgcgcttc ctggaaatga aggtcgtggt
    37021 tacgttcttc gtcgtcttct ccgtcgtgcg gttatgcacg gtcgccgtct tggcatcaac
    37081 gaaactttcc tttacaaatt ggttccgact gttggacaaa tcatggaaag ctactaccca
    37141 gaagtgcttg aaaaacgtga ttttatcgag aaaatcgtta aacgtgagga agaaacattt
    37201 gctcgtacta tcgatgcagg tagcggtcac ttagattcat tgcttgcgca gcttaaggct
    37261 gaaggtaagg atactcttga aggtaaagat atcttcaaac tttatgatac ttatggattc
    37321 ccggttgaat tgacagagga attggcagaa gatgcaggct acaagattga ccacgaaggc
    37381 tttaagtcag ccatgaaaga acaacaagac cgtgcgcgtg cagctgttgt taagggtggt
    37441 tcaatgggga tgcaaaatga aaccctagct ggtattgttg aagaatcacg attcgaatac
    37501 gacacatata gtcttgaatc aagtctttca gtcatcatcg ctgataatga acgtaccgaa
    37561 gctgtttcag aaggtcaagc ccttcttgtc tttgctcaaa caccattcta tgctgaaatg
    37621 ggtggacagg ttgctgacac aggtagaatc aaaaatgata agggtgacac agttgctgag
    37681 gttgttgatg ttcaaaaagc accaaatggt caacctctac acactgtaaa cgttttagca
    37741 tcactttcag ttggaacaaa ctacacactt gaaatcaaca aagagcgtcg tttggctgtt
    37801 gagaaaaacc acacagctac tcacttgctc catgcagctc ttcacaatgt tatcggtgaa
    37861 cacgcaactc aggctggttc attgaacgaa gaagaattct tgcgctttga ttttactcac
    37921 tttgaagcag taagcaatga ggaacttcgt cacattgaac aagaagttaa tgagcaaatt
    37981 tggaacgctc ttacaatcac aacgactgaa actgacgttg aaaccgcaaa agagatggga
    38041 gcaatggcgc tttttggtga gaaatatggt aaagtggttc gtgtggttca aattggtaat
    38101 tattctgttg aactttgtgg tggaactcac ttaaataatt cttcagaaat cggtctcttc
    38161 aagattgtca aagaagaagg tattggttca ggcactcgtc gtattattgc agttactggt
    38221 agacaagctt ttgaagctta tcgtaaccaa gaggatgccc taaaagagat cgctgctact
    38281 gtaaaagctc cgcaattgaa agatgcagca gctaaagtac aagctcttag cgactcgctt
    38341 cgtgatcttc aaaaagaaaa tgcagaactt aaagaaaaag cagcagctgc agcagctggt
    38401 gatgtcttta aagatgttca agaagctaag ggcgtgcgct tcattgctag tcaagttgat
    38461 gttgcagatg caggggcact tcgtacattt gctgataact ggaaacaaaa agactactct
    38521 gatgtgcttg ttctcgtagc agctattggt gagaaggtta atgtccttgt tgcaagcaaa
    38581 accaaagatg tccacgctgg taacatgatc aaagaattgg caccaattgt agcaggtcgt
    38641 ggtggaggta aaccagacat ggctatggca ggtggtagcg atgcaagtaa aattgcagag
    38701 ctgctagcag cagttgctga aatagtgtaa atccataaaa aaccaagtta tttagaactt
    38761 ggttttttat atttgaaatg actaccatca taaccttttt gactattatc gttgtcaaaa
    38821 agaaaaagat atgttacaat tatagtaaca aatagacatg aggaggtgtt agagaaaatg
    38881 acacatggta tcaaacttaa gaggcagatt tgcttatggg caagtatcct gactcttttc
    38941 attttaagtt atcatcttat cagggtaatc tggtttggag tacccttgag ttatctggtt
    39001 ttgggcttta caggactagc cttgctgttg aatacttttt cttattttag aaagtaagtt
    39061 ataagttaaa ggagaatgaa ggagatggca atggaggtta ttcttaaaaa tcggctcaaa
    39121 gaattacgag ctagagatgg aattaatcaa acggaaatgg ctaaactggc aggagtatcg
    39181 aggcagacca tcagtctcat tgagcgtaac gagtacaccc catcagttat aattgccatg
    39241 aaaattgcaa aggtatttca ggaaccagtt gaagaggtgt ttcgtctagt ggaggtcgag
    39301 gaatgaaaaa gaagaagaat agttttttac gcttgttaaa aatgtcattg ctatcaagtt
    39361 tggcgggagg gattataggt ggcatggttg gagccttttt aggttaccat ggagggagat
    39421 tagatcatct cacgttccta aaagatgatg tgattaacct gattatctta ttaaatcgtc
    39481 tagtagttgt cacagacctc acactttctt ttgttttttt aacgcaactg aaaaaagaga
    39541 cagctgttta taacacaatt gaagaggatg atatttctga aaatggttac cgtcagctga
    39601 ataaaaagca tgcttatacc atgttattga ttgccgtagc ttctatttta tccatgtgca
    39661 atgtgctact tggtctgacc ttaactaatg attcacagca tgccatgctg gccattccgc
    39721 ttctggatat ccttttatta ttaatggtca ttccttttca agctttagcc atgaaacgtt
    39781 acaatgccat tcggggaacc gatgttcctt attttccaaa tttgaaagaa ttaaaacata
    39841 atatcatggc tctagatgag gcagagttac aagcctatca taagacatcg tttgaaagtg
    39901 tgttgtcatt aaatggagtc atcatccctt ccttgtatgt gattttattt tttgtctatc
    39961 tattcactgg tcaagtggag ttaacagcca tcttagtttt ggtgttaatt cagctctatt
    40021 tactcgttaa gagtgcaaca atgaccagac aattttaccg ttagcctaaa aggagactgt
    40081 ttatgaaagg attcattaat tatttaaaaa tagcagtgct cattattctg gctatggttt
    40141 tcaatgtcct tccgatgatt ttattgcaaa agcaacacga tattcctatg gtacttaatt
    40201 ggggaattgg tattttctac ttggttattg ttggaagtgt ccttattgta ttatggcgtc
    40261 tttatcaagc taagcaagac acctctatta aacagcaaaa aatgagattg gttgactggg
    40321 gttatttagc attattttgg ttaatcatcc gtgtgatagc tattgtaggt acccttgtta
    40381 accagctatg gtctggtcaa caagtgagtg ctaatgatgc tgcaatacat accttagcta
    40441 gacttatcaa aggtggtttc ccgctttata ctgccctatt tgtacttgtg atagctttta
    40501 tcgctcctat tatggaagaa ctagtcttta gaggatttcc tatgattgat ctcttcaaag
    40561 gaaaatcact taaggtggca ggtttagtga cctctcttgt ttttgcttta ccacatgcca
    40621 ccaatagtgt tgaatttatc atgtacagct gtatgggcat ttttctcttt gttgcctatc
    40681 aaagacgagg aaacttaaaa gatgctatct tgttacatat ttttaataac ttgattgaag
    40741 tcattttgtt aatgtcaata ggcttaggag tcatataata aaaagcttcg gatggatgaa
    40801 ctgcacccca aaagttggac aaaaaaacta acatttggtt ggacttgtca agaaaaaata
    40861 gacataaagt taagctaatg agtaactatt tttttcaaat tcaacaggtg agagataccc
    40921 taaagctgaa tgcagacgtt tggggttata gtaagtctca ctatatttaa aaatctcaag
    40981 ctgagcttgc ttaatagttg cgaaatgagc atcattgaca agctctcttt ttaatgtttt
    41041 ataaaaggat tccatgagtg cattatcgta tggattacct tttcgattca tactggactt
    41101 acattttttc tgtctaagta ggtcctgaaa tcatgctccg gtatattgag aaccctgatc
    41161 tgtatggacg attacccctt ctttaggctt ttctctatta taagcttgat tgaaagcttc
    41221 tgttaccaac ttgtcttgca tacgttagcc cattgcccaa ccaacaacct ttctactata
    41281 aacgtctatc aagacagaca cataaagtat tccttcttgt gtaggaatgt aggttaggtc
    41341 tcctagccat aatttattct taccagtcgc ttggaaacat tgattaacaa gattgggacg
    41401 agttcatgaa gaacgtcgac ggttatagta tttatattga tatcgacttc ctttagcata
    41461 gagacctagc tgatgaagga gtctaccaac acgtttataa ttgacatgga tgccacgtct
    41521 acaaagttct tgggtaattc gtacacttcc gtaacgctct ttatgttcat agaaaatggc
    41581 tttaatcatt tcctttaaag cttcattctc aacatgcctt gaagagggac gacgttttaa
    41641 ataagcatag aaacctgaac gggagacttg aagagtttga caggtgtgat gactattcaa
    41701 gctaacacta ttttccttca gaagctgaaa ttttatttcc ggtttggctt caaaaagacc
    41761 tggaactttt ttagaagagc taattcctcc tgtagcagtt tgtgctcttt ttcaagtttt
    41821 tttgtcttaa attgagcatg acgaagtgcg ctcccatgtc ctgggaacgc actttctcca
    41881 tatttttcat attcttgaat ccattgataa agactattgg gatgaatttc taaagttgaa
    41941 ctaaccattt ttacaggctg ctcttcctca aggatgagtt ttacagcaga gagtttgaag
    42001 gctttatcaa aggtttttct tggcataatg acttttcctc gtttccactt aattttgtgt
    42061 ctactttatt ataccaagtc caactttgct taaaatataa tgtccaactt ttggggtgca
    42121 gttcatccat ccgaagcttt ttattaaaaa agtaattaac taatcattta aggatgaaac
    42181 gataaaactg atagctaaaa taaaaaatga ggttagctaa tgctaaaggg ataatcatta
    42241 gtaacgattg gcctgtaatt tcaaccagaa aaactggtgc cgccaagggt atcttattga
    42301 tcacacctaa aaagattgtt gcaccaacca taatggctaa cgtatttgat agagacaagg
    42361 gaataaggta ctgactaagg atagctagta gtaagccact agcacctcca agcatcatag
    42421 aaggagcaat tttaccccct tttgctccag aagcaaaaac taaagagata gcgacagctt
    42481 tggcaacaag tagccagcta atatagctca gatgtggttc ttcatggaga aaaaagagta
    42541 atccagcttt gccatttcct aaaatctctg ggaagaaaat gctgagtcct gcgataaggc
    42601 taaaagcgat gagaactttc ggaataaacc actgactttt ggcatcagcc ttaggaaggt
    42661 gtttcaaaag atgtttgtaa gcgtgagcaa agagggaaag gataagacca gccaggacgg
    42721 acccaataag ggttcctagc gtccaatgtt gctgaggcat taggtattga atctcatgtc
    42781 ggccttgtaa taaagcaact gtttccacag ccacatagct cgttaggcag gcagcgtata
    42841 tatttttaag ggaccaacgg ttaagaatag cttctaagat aaataaaata gtggctaagg
    42901 gagcattgta gacagcacca agggcagcgc cagaagcgca ggctaataaa agtttttgat
    42961 cggcttttga caagttgcat ctttggctcc aaagagacgt caaggtaaca gcgacttccc
    43021 gtgaagctcc ttctcggccg acaggagctc ccatagatac ggtcgttaat tggagccaac
    43081 catgccaaaa ctgtgtccag ggactaaatg agatgtcatc ttgaatgatt tgctgaatag
    43141 attgaatgtc ctttcctttt ttagccaata ggtgccaccc aagacctgca agaagtccag
    43201 caaagaggag tgaaagagct cgtctttgtg gtggaacact agcaatcata gtgctaaaag
    43261 aaccttgact gaatccaaaa ctgagggact gaatcaggtg aatggctttt gttaagataa
    43321 aagcaacaag gccagctgtt aaacctgtta aaaagaggta aaaggtcaag agtaaaaagt
    43381 gtcgtttcat aagactatta taacaaaagt taagaaaaga gactgataga ttatcagtct
    43441 cgtgagcact attagagtcc ataattataa tcgtcatcag acatagcttc tacagaacca
    43501 agaagatagc cattccctac ctgtgagaaa aagtcatggt ttgatgttcc tgttgaaata
    43561 ccattcataa caattgggtt aacatcgttt gcagtatctg ggaataaagg atcttgtcct
    43621 aaattcataa gagctttgtt agcattgtag cgtaaaaagg tcataacttc ctcagtccac
    43681 cctacgccat cataaagcgt tttggtgtat ttttcttcat tttcatacag ctgataaagg
    43741 aggtcataca tccaatcacg aaaattctct tgctcatctt ctggtagttc gttaaaacca
    43801 agctggaatt tgtaaccgat ataagtacca tgtacagatt catcacgaat aattagttta
    43861 atgatttcag caacatttgc tagcttatta tttcccaaat agtaaagagg ggtgaaaaag
    43921 ccagaataaa aaaggaaagt ttcgagatat gttgaggcaa ctttcttttg taaggcatct
    43981 ccattagcgt aaatgtcatt gataatacgt gctttttctt gaagaaactc attattatta
    44041 gtccactcaa agatctcttc aatttctttt ttggtgttta aggttgaaaa gatagacgaa
    44101 taagatttag cgtgaacaga ttccatgaat tgaatattgt ttaagacagc ttcttcgtga
    44161 ggagtgcgaa catcagcacg aatagcttcg acaccagttt ctgattgcat ggtatcaagt
    44221 agggttaatc ctccaaaaac cttgccaaca agatcttttt cttgaagaga aagtttgcgc
    44281 caatcgtcta agtcatttga taaagggata cgggtatcaa gccaaaattg ttcggtcaac
    44341 ttttcccaag ttgacttgtc aatgacgtct tcgatttcat tccagttaat tgcttcataa
    44401 tatgtagtca tagaaaaatg atctcctaaa gagtagtaaa gtgcgttaag gatatcgaga
    44461 taaaccagta gaaccgacca aagttctacc ggtaataata agtttaaata gattcaaaag
    44521 ggtaacgaat gagttagtga gtggtttagg cagatcgcct aaagcgattg cccaagaagt
    44581 aatagatgtt ttagcatcat tagggcatat cgaggaatta cgacacggag taaggaaagt
    44641 cgataatact ttccttacgt agttagtgag tggtttaggc agatcgccta aagcgactgc
    44701 cgtagaagta atagatgctt tagcatcatt acttcttaac caattaaatg acacaagatt
    44761 cacattggtt tgcgcccact tcttccccat catccgtaaa ggtacggata tagtagatag
    44821 atttaatgcc tttattgaaa gcgtagtttc gtaagatgga taaatcacga gtggtttgtt
    44881 tgctttgtgt tttccactca taaagctcca taggcaactc actacgaagg aatagagtta
    44941 atgacaagcc ttggtccaca tgttcggtcg cagcggcata gacatcaata actttgcgca
    45001 tgtccatatc gtaagcagat gtatagtaag gaatggtatc cgtagacaaa ccatttgcag
    45061 gatagtagat tttaccaatt ttcttttctt gacgctcttc gatgcgttgt gtgattgggt
    45121 gaatagaagc agagcagtca ttgatataag aaatagagcc atttggagca actgctagtc
    45181 ggttttgatg ataaagacca tctttttgaa cggcgtcgcg aagagcctcc caatctgaag
    45241 cttgcggaat aaaatggtct ttgaacagat ctttcaccaa atcagatttt ggaacaaagt
    45301 gtcctgtaac gtatttatca aagtaactac cattagcgta cttagagttc tcaaagccaa
    45361 caaaggtagt ttggcgctca cgagcgatgt tattggattc gaccaaggtc caataattca
    45421 ggagcataaa gtaaatatca gtaaactcga tggattctgg actgccatat tcaatatgat
    45481 gttgagcaag gtaagaatgt agtcccatag ctccaaggcc aaaagtatga gcttggctat
    45541 tgccatgttt aatggttgga acagcttcaa tgcttgatga atcagtaaca aaagttaggg
    45601 cacgtgtcat ggtcttaata gaacggccaa agtctggtga ggtcatcatg ttcaggatat
    45661 tagtggaacc taagttacat gaaatatcag ttcccatttc tacaaactct tgcgcatcat
    45721 tgataaggct aggtgtttga acttgtaaaa tttcagaaca caagttgctc atgatgattt
    45781 ttccatcgat aggattagct ttattagctg tatcaatatt gatgatataa gggtaaccag
    45841 attcttgttg taattttgaa atctctgttt caagatcacg agctttaatt ttagtcttag
    45901 taattttagg gttcgccact aactcatcgt acatattggt aatgtcgaga tagttaaagg
    45961 gaatgccata ttctttttca acattgtaag gactaaagag atacatgtcc tcgtttttac
    46021 gagctaattc gtagaattta tcaggaacgg taatccctag tgacaaggtt ttaacacgca
    46081 ccttttcatc ggcattttct tttttagtag ataagaaagc aatgatatca ggatgaaaaa
    46141 catttaggta aacaacacca gctccttgac gttgcccaag ttgatttgaa taagaaaaac
    46201 tatcttcaaa taatttcata acaggaacaa ctcctgaggc tgcaccagca tagcctttga
    46261 ttggtgcgcc agcttcacgg aggttagaca aggtaatccc aactcctcca ccaatacggg
    46321 ataattgcaa agcagagttg atagaacgtc cgatagagtt catgtcatca gttacttgaa
    46381 tcaagaaaca agagaccaat tcaccacgac ggcttcgacc agcatttaaa aaggaaggag
    46441 tagccggttg gtaacgttgg ttaatcattt caacggctaa atcttttgct aagtcttctt
    46501 gaccatctgc aaaatacaaa gcattaaaca agacacggtc ttcaaggttt tctaaataat
    46561 gctctccatc atttgttttt aaggcgtatt gctggtagaa cttgtaggct gccataaatg
    46621 atttaaagcg aaaattttct gatttgatta tgctatctaa ttcagtaata aattcagggc
    46681 ggtatttctg aataaaagct gattcaatgt aatcatttga gattaaatac tcaatttttt
    46741 ccgtaatgga agtaaaagac atggtgtttg gcagcacatt ttcagcggaa aaagctttta
    46801 aagcttcttt gtctttatga agtggaattt taccattaac aggacggtta atttcattat
    46861 ttaggcgaaa atatgaaata tcgccaagat ctttgagact catattattt ttccttgtct
    46921 tattaaatca attcttttaa ctttgcaggt tggaagccag aaaatacgag gttatctgct
    46981 tcgataacag gagcagatgt gaagccaaga cttttcacat aatccacttt ttcaggatgt
    47041 tcatcgatat taatttcctg gaagttggct ccgtgttgtt ctaaaaattt cttagtcatt
    47101 ttgcactgca tgcaattatt tttggaatat actgtaatca tgtcttgtcc ttctaaaatt
    47161 agtaatggtt ccatct
//
LOCUS       DAXWIQ010000012        39909 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000012 DAXWIQ010000000
VERSION     DAXWIQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39909
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.012"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            241..1473
                     /gene="pbp3"
                     /locus_tag="ACGEUI_000633"
     CDS             241..1473
                     /gene="pbp3"
                     /locus_tag="ACGEUI_000633"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase PBP3"
                     /protein_id="HFF8165490.1"
                     /translation="MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLY
                     EKDAKEVVPVASVSKLLTTYLVYKEVSKGKLNWDSPVTISNYPYELTTNYTISNVPLD
                     KRKYTVKELLSALVVNNANSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNST
                     GLTNHFLGANTYPNTEPDDENCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFAGQTI
                     YSYNYMLKGMPCYREGVDGLFVGYSKKAGASFVATSVENQMRVITVVLNADQSHEDDL
                     AIFKTTNQLLQYLLINFQKVQLIENNKPVKTLYVLDSPEKTVKLVAQNSLFFIKPIHT
                     KTKNTVHITKKSSTMIAPLSKGQVLGRATLQDKHLIGQGYLDTPPSINLILQKNISKS
                     FFLKVWWNRFVRYVNTSL"
     gene            1641..2822
                     /locus_tag="ACGEUI_000634"
     CDS             1641..2822
                     /locus_tag="ACGEUI_000634"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFF8165491.1"
                     /translation="MTKRIILTIFTFICFSVMPLVHAEDVMDITRQAGYTVSEVNRPK
                     SSIVVDANSSDILWQDNIDIPRDPASMSKMFTLYILFEELAKGKITMDTTITATPTDQ
                     AIANIYEISNNNIVAGVAYPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRVNA
                     TAKQLGMTNTHFSNASGAAAQAFQGYYNPTKYDLSASNITTARDLSKLLYAFLKKYPE
                     IISFTNKSVVHTMVGTPYEEEFHTYNHSLPDNQFGMKGVDGLKTGSSPSAAFNAMITA
                     KRGKTRLITIVMGVGDWSDQNGEFYRHPFVNALTEKGFKDSKTLSKKARQKLEKLVPQ
                     TKKETSSKQQHFKATKKQSYLERVEDFMNHNHTFLLICLAIFIITILLLSLVVFAMGR
                     Q"
     gene            complement(2974..4392)
                     /gene="sufB"
                     /locus_tag="ACGEUI_000635"
     CDS             complement(2974..4392)
                     /gene="sufB"
                     /locus_tag="ACGEUI_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFF8165492.1"
                     /translation="MSDINEKVEPKPIDLGDYQFGFHDDVEPIYSTGKGLSEAVVREL
                     SAAKNEPEWMLEFRLKSLETFNKMPMQTWGADLSDINFDDIIYYQKASDKPARSWDDV
                     PEKIKETFDRIGIPEAERAYLAGASAQYESEVVYHNMKGEFEKLGIIFTDTDSALKEY
                     PDLFKQYFAKLVPPTDNKLAALNSAAWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRARALTDATVEWIDGNLGAKTTMKYPSVYLDGPGARGTMLSIAFANAGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKSGGKVDYRGQVTFNKQSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(4385..4864)
                     /gene="sufU"
                     /locus_tag="ACGEUI_000636"
     CDS             complement(4385..4864)
                     /gene="sufU"
                     /locus_tag="ACGEUI_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFF8165493.1"
                     /translation="MALSKLNHLYMAVVADHSKRPHHHGQLDGVEAVQLNNPTCGDVI
                     SLTVKFDEDKIEDIAFAGNGCTISTASSSMMTDAVIGKSKEEALALADIFSEMVQGQE
                     NPAQKELGEAELLAGVAKFPQRIKCSTLAWNALKEAIKRSANAQHLTDQNVKEGKNV"
     gene            complement(4851..6077)
                     /locus_tag="ACGEUI_000637"
     CDS             complement(4851..6077)
                     /locus_tag="ACGEUI_000637"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFF8165494.1"
                     /translation="MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQS
                     YYQEDNANVHRGVHTLAERATLKYEASRQQVADFIHAKSSKEVLFTRGTTTSLNWVAR
                     FAEQVLTPEDEVLISIMEHHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLANKLTT
                     KTRFVSLVHVSNVLGCINPIKEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFA
                     FSAHKMLGPTGLGVLYGKEELLNQVEPLEFGGEMIDFVYEQEATWKELPWKFEAGTPH
                     IAGAIGLSAAISYLQRLGMADIHAHEAELIAYVLPKLEAIEGLTIYGPSQPSARSGLI
                     SFNLDDLHPHDLATALDYEGVAVRAGHHCAQPLLSYLGVPATVRASFYIYNTKADCDR
                     LVEAILKAKEFFNGTL"
     gene            complement(6108..7370)
                     /gene="sufD"
                     /locus_tag="ACGEUI_000638"
     CDS             complement(6108..7370)
                     /gene="sufD"
                     /locus_tag="ACGEUI_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFF8165495.1"
                     /translation="MTKEKLVAFSQAHAEPAWLQERRLAALEAIPNLELPTIERVKFH
                     RWNLGDGTLTENESLASVPDFIAIGDNPKLVQVGTQTVLEQLPMALIDKGVVFSDFYT
                     ALEEIPEVIEAHFGQALAFDEDKLAAYHTAYFNSAAVLYVPDHLEITTPIEAIFLQDS
                     DSDVPFNKHVLVIAGKESKFTYLERFESIGNATQKISANISVEVIAQAGSQIKFSAID
                     RLGPSVTTYISRRGRLEKDANIDWALAVMNEGNVIADFDSDLIGQGSQADLKVVAASS
                     GRQVQGIDTRVTNYGQRTVGHILQHGVILERGTLTFNGIGHILKDAKGADAQQESRVL
                     MLSDQARADANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLDQETAERLVIRGF
                     LGAVIAEIPIPSVRQEIIKVLDEKLLNR"
     gene            complement(7465..8235)
                     /gene="sufC"
                     /locus_tag="ACGEUI_000639"
     CDS             complement(7465..8235)
                     /gene="sufC"
                     /locus_tag="ACGEUI_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFF8165496.1"
                     /translation="MSILEINNLHVSIEGKEILKGVNLTLKTGEVAAIMGPNGTGKST
                     LSAAIMGNPNYEVTQGQILLDGVNILDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAMNAGKADEDKISVRDFITKLDEKMALLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGKDFGAMIITHYQRLLNYITPDLVHV
                     MMDGRIVLSGDAALATRLEKEGYAGIAQDLGIEYKEES"
     gene            complement(8357..9526)
                     /locus_tag="ACGEUI_000640"
     CDS             complement(8357..9526)
                     /locus_tag="ACGEUI_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFF8165497.1"
                     /translation="MFSFTIDYVLVLIGALLMSLFLTPLVRFLAFRVGAVDNPNARRV
                     NKVPMPTSGGLAIFMSFLVASLGLIPIASKGAMFFGQTYFSYILPVVIGATVITLTGF
                     LDDLYELSPKLKMFGILIGAVIVWAFTDFKFDSFKIPFGGPLLVFGPFLTLFLTVLWI
                     VSITNAINLIDGLDGLVSGVSIISLVTMAIVSYFFLPQKDFFLTLTILVLISAIAGFF
                     PYNYHPAMIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPVIILGVPIMDTIVAII
                     RRSLSGQKFYEPDKMHLHHRLLSMGFTHRGAVLVVYGITMLFSLISLLLNVSSRIGGV
                     LLMLGLLFGLEVFIEGLEIWGEKRTPLFNLLKFIGNSDYRQAMLLKWKEKKDLKH"
     gene            complement(9533..10294)
                     /gene="mecA"
                     /locus_tag="ACGEUI_000641"
     CDS             complement(9533..10294)
                     /gene="mecA"
                     /locus_tag="ACGEUI_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFF8165498.1"
                     /translation="MEMKQISETTLKITISMDDLEERGMELKDFLIPQEKTEEFFYSV
                     MDELDLPDNFKDSGMLSFRVTPRKDRLDVFVTKSEINKDINLEDLAEFGDMSQMTPED
                     FFKSLEQSMREKGDVKAHEKLEKIEEIMEDVVEATLANQSEAADPSTNHESEPLDYVH
                     YVLDFSTITEAVAFAKTIDFSIEASELYKGSNCYHMTILLDVQQQPSYFANVMYARLI
                     EHANPGSKTRAYLQEHGLQLMLDGAVEQLQKIELG"
     gene            complement(10440..11279)
                     /locus_tag="ACGEUI_000642"
     CDS             complement(10440..11279)
                     /locus_tag="ACGEUI_000642"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFF8165499.1"
                     /translation="MLIIELLKAIFFGIIEGITEWLPVSSTGHLILVQEFIRLNQDKA
                     FIEMFNIVIQLGAIIAVMLIYFERLNPFQPGKTAREVQLTWQLWLKVVIACIPSILIA
                     VPLDNWFEAHFYFMVPIAIALIVYGIAFIWIEKRNAQQEPAVTELARMSYKTAFFIGC
                     FQVLSIVPGTSRSGATILGAIILGTSRTVAADFTFFLAIPTMFGYSGLKAVKFFLDGH
                     HLDFAQVLILLVASLTAFVVSLLAIRFLTDYVKKHDFTIFGKYRIVLGSLLLIYSFFK
                     FVF"
     gene            complement(11345..13243)
                     /locus_tag="ACGEUI_000643"
     CDS             complement(11345..13243)
                     /locus_tag="ACGEUI_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFF8165500.1"
                     /translation="MKKILMTLVLCFSLLGIRIKAADVDYSITNYEGQLLLSKENTAR
                     FEQKVTYQFDTSYNGQYISLGRTGHLPAGFAIDQKPKVEVYQNGQQVPVSQEFSDLGD
                     GYRLKLYNAGQAGDKVDVKVIWQLHHLLTAYQDVAELNWTPISDWDKTLEKVSLTVTT
                     PTDIQDSNLWAHRGYYQKKPQVLKEGNSRYQINAKNVSGQLELHAYWDKKALLGKEPV
                     DVSTSKKNKIVALETKISRRRTLLQLLFGKVIPLVEVGFLLWQLIQFTRLKKQFNRYH
                     LANHTDHSYEVPEDLSPLVLTQAIYGQSFAYLSPTASESQKLLIPKGVTFEALVQATL
                     LDLIDQKVLLLTKEEGKAYLEISQLDRVTDEEAAFLDMAFGNKVTLPVDQLFSQYHYD
                     ADTIKQLKKTYKGKKLEQEVRQSSEQVIKAMKKASAAITNNVLETIKKLNLPDTYRQM
                     TPAEKRKSNSVQGLGCLLLILNSGLLIYLAIKESGLALIYLALMVLTMCLGFYISLKL
                     DQYKKLGIETPEGGVRLHQWQSFKNMIRDIDKFEDVAIEGLVVWNRVLVYATLFGYAK
                     KVERYLKVHRIALPEVYQAVRPGELSMVMYATTPTFVSSLSSATTSSNFSVSSGGGIS
                     GGGGGGAF"
     gene            13441..15009
                     /locus_tag="ACGEUI_000644"
     CDS             13441..15009
                     /locus_tag="ACGEUI_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFF8165501.1"
                     /translation="MKKLILSCLVALALLFGGMSRAQANQYLRVGMEAAYAPFNWTQD
                     DASNGAVPIEGTSQYANGYDVQVAKKVAKAMNKELLVVKTSWTGLIPALTSGKIDMIA
                     AGMSPTKERRNEISFSNSYYTSQPVLVVTANGKYADATSLKDFSGAKVTAQQGVWHVN
                     LLTQLKGAKLQTPMGDFSQMRQALTSGVIDAYISERPEAMTAEAADSRLKMITLKKGF
                     AVAESDAAIAVGMKKNDDRMATVNQVLEGFSQTDRMALMDDMVTKQPVEKKAEDAKAS
                     FLGQMWAIFKGNWKQFLRGTGMTLLISMVGTITGLFIGLLIGIFRTAPKAKHKVAALG
                     QKLFGWLLTIYIEIFRGTPMIVQSMVIYYGTAQAFGISIDRTLAAIFIVSINTGAYMS
                     EIVRGGIFAVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFVLTFTVTRILRYIERRFDADTYT
                     TGANQMQIAEVSNV"
     gene            15002..15742
                     /locus_tag="ACGEUI_000645"
     CDS             15002..15742
                     /locus_tag="ACGEUI_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8165502.1"
                     /translation="MSNSIIEIKNLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STLLRSINLLEEPSAGQILFHGEDVLAEHYNLTHYREKLGMVFQSFNLFENLNVLENA
                     IVAQTTVLKRDRAQAEQIAKENLNAVGMTEQYWQAKPKQLSGGQKQRVAIARALSVNP
                     EAMLFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFARDVSDRIIFMDKGL
                     ITEEGSPQQIFENPTQDRTKEFLQRFLK"
     gene            15884..16000
                     /locus_tag="ACGEUI_000646"
     CDS             15884..16000
                     /locus_tag="ACGEUI_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFF8165503.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(16226..17236)
                     /gene="gap"
                     /locus_tag="ACGEUI_000647"
     CDS             complement(16226..17236)
                     /gene="gap"
                     /locus_tag="ACGEUI_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFF8165504.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNIEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGNFIKVSAERDPENIDWATDGVEIVLEATGFFAK
                     KEAAEKHLHANGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGIQKGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVVTLDKNVSVDEINAAMKAASNDSFGYTED
                     PIVSSDIVGVSYGSLFDATQTKVMEVDGSQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
     gene            complement(17584..19662)
                     /gene="fusA"
                     /locus_tag="ACGEUI_000648"
     CDS             complement(17584..19662)
                     /gene="fusA"
                     /locus_tag="ACGEUI_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFF8165505.1"
                     /translation="MAREFSLAKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQTLHDRLQANAH
                     PIQLPIGAEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPEEYLEQAQEYREKLIEAV
                     AETDEDLMMKYLEGEEITNDELIAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVIA
                     YLPSPLDIPAIKGVNPDTDAEEERPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVMNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKVILESIEVPEPVIQLMVEPKSKADQDKMGVALQKLAEEDPTFRVETNVETGETVIA
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLIESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGE"
     gene            complement(20029..20499)
                     /gene="rpsG"
                     /locus_tag="ACGEUI_000649"
     CDS             complement(20029..20499)
                     /gene="rpsG"
                     /locus_tag="ACGEUI_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFF8165506.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFNAIKEATGNDALEVFETAMDNIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            complement(20520..20933)
                     /gene="rpsL"
                     /locus_tag="ACGEUI_000650"
     CDS             complement(20520..20933)
                     /gene="rpsL"
                     /locus_tag="ACGEUI_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFF8165507.1"
                     /translation="MPTINQLVRKPRKSKIEKSDSPALNIGYNSHKKVQTKMAAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            complement(21141..23762)
                     /locus_tag="ACGEUI_000651"
     CDS             complement(21141..23762)
                     /locus_tag="ACGEUI_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC10/PgrA surface exclusion domain-containing
                     protein"
                     /protein_id="HFF8165508.1"
                     /translation="MDLEQTKPNQVKQKIALTSTIALLSASVGVSHQVKADDRASGET
                     KASNTHDDSLPKPETIQEAKATIDAVEKTLSQQKAELTELATALTKTTAEINHLKEQQ
                     DNEQKALTSAQEIYTNTLASSEETLLAQGAEHQRELTATETELHNAQADQHSKETALS
                     EQKASISAETTRAQDLVEQVKTSEQNIAKLNAMISNPDAITKAAQTANDNTKALSSEL
                     EKAKADLENQKAKVKKQLTEELAAQKAALAEKEAELSRLKSSAPSTQDSIVGNNTMKA
                     PQGYPLEELKKLEASGYIGSASYNNYYKEHADQIIAKASPGNQLNQYQDIPADRNRFV
                     DPDNLTPEVQNELAQFAAHMINSVRRQLGLPPVTVTAGSQEFARLLSTSYKKTHGNTR
                     PSFVYGQPGVSGHYGVGPHDKTIIEDSAGASGLIRNDDNMYENIGAFNDVHTVNGIKR
                     GIYDSIKYMLFTDHLHGNTYGHAINFLRVDKHNPNAPVYLGFSTSNVGSLNEHFVMFP
                     ESNIANHQRFNKTPIKAVGSTKDYAQRVGTVSDTIAAIKGKVSSLENRLSAIHQEADI
                     MAAQAKVSQLQGKLASTLKQSDSLNLQVRQLNDTKGSLRTELLAAKAKQAQLEATRDQ
                     SLAKLASLKAALHQTEALAEQAAARVTALVAKKAHLQYLRDFKLNPNRLQVIRERIDN
                     TKQDLAKTTSSLLNAQEALAALQAKQSSLEATIATTEHQLTLLKTLANEKEYRHLDED
                     IATVPDLQVAPPLTGVKPLSYSKIDTTPLVQEMVKETKQLLEASARLAAENTSLVAEA
                     LVGQTSEMVASNAIVSKITSSITQPSSKTSYGSGSSTTSNLISDVDESTQRALKAGVV
                     MLAAVGLTGFRFRKESK"
     gene            complement(23753..24592)
                     /gene="purR"
                     /locus_tag="ACGEUI_000652"
     CDS             complement(23753..24592)
                     /gene="purR"
                     /locus_tag="ACGEUI_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFF8165509.1"
                     /translation="MKLRRSERMVVISNYLINNPYKLTSLNTFATKYEAAKSSISEDI
                     AIIKKAFEEANIGDIDTLTGASGGVIFTPSISETEARTIVEDLCQRLSESDRILPGGY
                     IYLSDLLSTPKILQNIGRIIANAFKGEKIDAVMTVATKGVPLANAVANILSVPFVIVR
                     RDLKITEGSTVSVNYASASSDRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTITGMISL
                     LTEFDSTLVGVAVFAENAQSEREQMTFKSLLKVSEIDVKNNNVVVEVGNIFDKWKKER
                     EWT"
     gene            complement(24859..25797)
                     /locus_tag="ACGEUI_000653"
     CDS             complement(24859..25797)
                     /locus_tag="ACGEUI_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HFF8165510.1"
                     /translation="MKINQMKKDQLFEGFYLIKSAEVRKTRAGKDFISLTFQDDTGEI
                     SGNLWDAQPYNVEEFTAGKVVFMKGRREVYNGTPQVNQITLRNVRPGEPNDPKDFKEK
                     APVSVTEVRDYLEQMLFKIENATWQRIVRALYRKYDKEFYTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPDLNKSLLFAGIMLHDLAKVIELTGPDNTEYTVRGNLIGHIS
                     LINEEITKVISELQIDDTKEEVIVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALSRVSEGEMTNRIFAMDNRSFYKPNY"
     gene            complement(25787..27058)
                     /locus_tag="ACGEUI_000654"
     CDS             complement(25787..27058)
                     /locus_tag="ACGEUI_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFF8165511.1"
                     /translation="MDLILFLLVLVLLGLGAYLLFKVNGLQHQLAQTLEGNADNLSDQ
                     MTYQLDTANKQQLLELTQLMNRQQAGLYQQLTDIRDVLHRSLSDSRDRSDKRLEKINQ
                     QVNQSLKNMQESNEKRLEKMRQIVEEKLEETLKNRLHASFDSVSKQLESVNKGLGEMR
                     SVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTSSQYEREFVTVSGSSERVEYAI
                     KLPGNGQGGYIYLPIDSKFPLEDYYRLEDAYEVGDKLAIEASRKALLAAIKRFAKDIH
                     KKYLNPPETTNFGVMFLPTEGLYSEVVRNASFFDSLRREENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNADDISKILGNVKLEFDKFGGLLAKAQKQMNTANNTLDQLISTRTNAI
                     VRALNTVETYQDQATKSLLNMPLLEEENNEN"
     gene            complement(27060..27692)
                     /locus_tag="ACGEUI_000655"
     CDS             complement(27060..27692)
                     /locus_tag="ACGEUI_000655"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFF8165512.1"
                     /translation="MSKVALFAGGDLSYISRDFDYFVGIDRGSLFLLENGLPLNMAVG
                     DFDSVSQKAFTDIKEKAELFITAHPEKNDTDTELALKEVFARFPEAEVTIFGAFGGRM
                     DHLLSNIFLPSDPGIAPFMAQIALRDQQNMITYRPAGQHLIHQEEGMTYVAFMAEGEA
                     DLTITGAKFELTQDNFFKKKIYSSNAFIHQPITVSLPSGYLIIIQSKDWS"
     gene            complement(27685..28347)
                     /gene="rpe"
                     /locus_tag="ACGEUI_000656"
     CDS             complement(27685..28347)
                     /gene="rpe"
                     /locus_tag="ACGEUI_000656"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFF8165513.1"
                     /translation="MSTLKIAPSILAADYANFASELARIEETDAEYVHIDIMDGQFVP
                     NISFGADVVASMRKHSKLVFDCHLMVVDPERYVEAFAQAGADIMTIHTESTRHIHGAL
                     QKIKAAGMKAGVVINPGTPATALEPLLDLVDQVLIMTVNPGFGGQAFIPECLEKVATV
                     AKWRDEKGLSFDIEVDGGVDNKTIRACYEAGANVFVAGSYLFKASDLVSQVQTLRTAL
                     NV"
     gene            complement(28357..29229)
                     /gene="rsgA"
                     /locus_tag="ACGEUI_000657"
     CDS             complement(28357..29229)
                     /gene="rsgA"
                     /locus_tag="ACGEUI_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFF8165514.1"
                     /translation="MQGKIIKSLAGFYYVESEGQVYQTRARGNFRKRGETPYVGDIVD
                     FSAEDNSEGYILAIHPRKNSLVRPPIVNIDQAVVIMSAKEPEFNSNLLDRFLILLEHK
                     AIHPVVYISKMDLLDSPEEIKAIGRQYQAIGYDFVTSLEELLPLLADKITVFMGQTGV
                     GKSTLLNRIAPELALEIGEISDSLGRGRHTTRAVSFYNTHGGKIADTPGFSSLDYDIA
                     NAEDLNEAFPELRRLSHECKFRSCTHTHEPKCAVKAALETGELWPVRYEHYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            complement(29653..30525)
                     /gene="rsmA"
                     /locus_tag="ACGEUI_000658"
     CDS             complement(29653..30525)
                     /gene="rsmA"
                     /locus_tag="ACGEUI_000658"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFF8165515.1"
                     /translation="MRIADYSVTKAVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLVPILADTLRDFDNVQVVNQDIL
                     KADLQTQIKQFKNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
                     SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVKD
                     EDFFFRVSRLSFVHRRKTLWNNLTSHFGKSEDIKAKLEKGLALADIKPSIRGEALSIQ
                     DFGKLADALKEVGL"
     gene            complement(30639..31229)
                     /gene="rnmV"
                     /locus_tag="ACGEUI_000659"
     CDS             complement(30639..31229)
                     /gene="rnmV"
                     /locus_tag="ACGEUI_000659"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFF8165516.1"
                     /translation="MQRGYSSLTEKINIQEVLVVEGKDDTANLRRFYEVDTYETRGSA
                     ITEEDLERINRLNDLRGVIVLTDPDYNGERIRKLIMAAVPTARHAFLNRNEAVPSSKS
                     KGRSLGVEHANFEDLQKALAHVTQQYDDESYFDIRQTDLIRLGLLMASDSRKRREYLG
                     EKLRIGYANGKQLLKRLELFGITLAEVEEVMETYEE"
     gene            complement(31201..31992)
                     /locus_tag="ACGEUI_000660"
     CDS             complement(31201..31992)
                     /locus_tag="ACGEUI_000660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFF8165517.1"
                     /translation="MTNKVEKLTIFDTHTHLNVAEFQGHETEELTLAQEMGVAYHNVV
                     GFDQATISGALTLANKYANIYATIGWHPTEAGSYSEAVEEAIVSQLSHSKVIALGEIG
                     LDYYWMEDPKEVQIEVFKRQMQLAKDHDLPFVVHTRDALEDTYEVIKAAGVGPRGGIM
                     HSYSGSLEMAERFIELGMMISFSGVVTFKKALDIQEAAQHLPLDKILVETDAPYLTPV
                     PKRGKQNHTAYTRYVVDKIAELRGMTVEEVAKATTANAKRVFKLD"
     gene            32277..33107
                     /locus_tag="ACGEUI_000661"
     CDS             32277..33107
                     /locus_tag="ACGEUI_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8165518.1"
                     /translation="MPKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEI
                     VKRLHISQAALTRFAKKCGFTGYRAFAFDYLHSLQESQETFQSIHLELTKRVLMDYDA
                     LINKTYELVNEEKLLNLAKLIDSSERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSD
                     TDGFTWANSLVNENCLVFGFSLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSL
                     DIIPVSSTHQLHYGNRVSPQFPLLIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNDL
                     "
     gene            complement(33218..34156)
                     /locus_tag="ACGEUI_000662"
     CDS             complement(33218..34156)
                     /locus_tag="ACGEUI_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8165519.1"
                     /translation="MKHYLAIDIGGTAIKYGLISETGDLLEKEEMATEAYKGGPSILE
                     KVKGLVKTYQDQMDLAGVAISSAGMVNPDEGEIFYAGPQIPNYAGTQFKKEIEETFGL
                     PCEVENDVNCAGLAEAISGSAKDYPVALCLTIGTGIGGCLLFNSQVFHGSSHSACEVG
                     YLHLSDGQFQDLASTTALVQEVVLAYGDDISQWDGRRIFEQAKAGDAICIAAISKQVD
                     YLGQGIANICYVVNPNVVVLGGGIMAQKDYLADKLKTALDSYLVSSLAKKTQLKFASH
                     GNNAGILGAYYHFKQKNERSCSFITLEKIENNLASS"
     gene            complement(34178..35092)
                     /locus_tag="ACGEUI_000663"
     CDS             complement(34178..35092)
                     /locus_tag="ACGEUI_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFF8165520.1"
                     /translation="MTDLTKYQGIIPAFYACYDDQGNISPERVRALTQYYIDKGVQGL
                     YINGSSGECIYQSVFDRQLVLENVMAVAKGKLTIINHVACNNTKDSIELAAHSERLGV
                     DAIAAIPPIYFRLPEYAVADYWNAISSAAPHTDFIIYNIPQLAGVALTPSLYKTMLAN
                     KRVIGVKNSSMPVQDIQTFCAIGGDDHIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPE
                     LFLRLNQLIADKDLEKAKALQYTINEIIGVLVSAHGNMYGVIKEVLRINEGLDIGSVR
                     SPLAELVEEDRVICQRAAALINQAKETF"
     gene            complement(35104..35766)
                     /locus_tag="ACGEUI_000664"
     CDS             complement(35104..35766)
                     /locus_tag="ACGEUI_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFF8165521.1"
                     /translation="MTSKKQGLLHSLFKLDSKWMRASAALFDLLVFNLLFVLSCLPLL
                     TIGVAKMALYASLLDWREGQVSQLVTTYSSHFKYYFKSGLRLGLIELGIMTICLLDLF
                     LIRNQSGLVFQGFKVLCVAVLFLVVILFLYAYPQAVKRDLSLSTLFKRSFLLAGLFFP
                     WSFAFLAFICLTIFSLQLSLLTLFGGVSLLAIIGISSLTYLYLIIMESLLRRFPLNND
                     IE"
     gene            complement(35923..36753)
                     /locus_tag="ACGEUI_000665"
     CDS             complement(35923..36753)
                     /locus_tag="ACGEUI_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8165522.1"
                     /translation="MTKKKLTASDILTTVMLCVLTILFIFPFYWIMTGAFKAQADTIM
                     IPPQWWPKAPTIENFKALVVQNPALKWLWNSVFISVATMFLVCGTSSLAGYALAKKRF
                     YGQRLLFSIFIAAMALPKQVVLVPLVRIVNFMGIHDTLAAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFFNVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRENLTISLGVATMQAEMATNYGLIMAGAAMAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(36766..37653)
                     /locus_tag="ACGEUI_000666"
     CDS             complement(36766..37653)
                     /locus_tag="ACGEUI_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8165523.1"
                     /translation="MNVNKLKMRETLISYAFLAPVLVFFVIFVLIPMIMGFVTSFFNY
                     SMTEFTFVGFANYARMFQDPIFMKSLINTLIIVIGSVPVVVFFSLFVAAKTYDKNVVA
                     RSFYRAVFFLPVVTGSVAVTVVWKWIYDPMSGILNYVLKYAHVIEQNISWLGDKHWAL
                     LAIIVILLTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWNIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIAIISFAQFKILGNDVEY"
     gene            complement(37756..39075)
                     /locus_tag="ACGEUI_000667"
     CDS             complement(37756..39075)
                     /locus_tag="ACGEUI_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8165524.1"
                     /translation="MNMKKLASLAMLGASVLGLAACGGKSQKEAGASKSDTAKTEITW
                     WAFPVFTQEKAEDGVGTYEKKLIAAFEKANPEIKVKLETIDFTSGPEKITTAIEAGTA
                     PDVLFDAPGRIIQYGKNGKLADLNDLFTEEFTKDVNNDKLIQASKAGDTAYMYPISSA
                     PFYMALNKKMLKDAGVLDLVKEGWTTDDFEKVLKALKDKGYNPGSFFANGQGGDQGPR
                     AFFANLYSSHITDDKVTKYTTDDANSIKAMTKISNWIKDGLMMNGSQYDGSADIQNFA
                     NGQTSFTILWAPAQPGIQAKLLEASKVDYLEIPFPSDDGKPELEYLVNGFAVFNNKDE
                     QKVAASKTFIQFIADDKEWGPKNVVRTGAFPVRTSYGDLYKDKRMEKIAEWTKFYSPY
                     YNTIDGFAEMRTLWFPMVQAVSNGDEKPEDALKAFTEKANKTIKKTQ"
     gene            complement(39124..39828)
                     /locus_tag="ACGEUI_000668"
     CDS             complement(39124..39828)
                     /locus_tag="ACGEUI_000668"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFF8165525.1"
                     /translation="MPDKPTKEKLMEQLKGGIIVSCQALPGEPLYSETGGIMPLMAKA
                     AQEAGAVGIRANSVRDIKEIQAITDLPIIGIIKKDYPPQEPFITATMTEVDQLAALNI
                     AVIAMDCTKRDRHDGLDIASFIRQVKEKYPNQLLMADISTFDEGLVAHQAGIDFVGTT
                     LSGYTPYSRQEAGPDVALIEALCKAGIAVIAEGKIHSPEEAKKINDLGVAGIVVGGAI
                     TRPKEIAERFIEALKS"
BASE COUNT        11940 a         8581 c         7339 g        12049 t
ORIGIN      
        1 aagaaaataa tgaaatgaaa aattatctga atatttcgta gaattttctt tattttatac
       61 atttatctat atgaaagcgt tacactcctt ttttaatgag gtttcaatta gtttaataaa
      121 gattgtcatt ctctcatggt agcgttttat tacatcacta ctcttgtgag gtaagtcatt
      181 accttagaca aaaatatggt ataattaaaa aaataatact tgagtaagga taatctataa
      241 atgatcaaac gattaatttc cctagtggtc atcgccttat tttttgcagc aagcactgtt
      301 agcggtgaag agtattcggt aactgctaag catgcgattg ccgttgacct tgaaagtggc
      361 aaagttttat acgaaaaaga tgctaaagaa gttgtcccag tcgcctcagt cagtaagctc
      421 ttgacaacct atctggttta caaagaagtt tctaagggca agctaaattg ggatagtcct
      481 gtaactattt ctaactaccc ttatgaactc actacaaact atactattag taacgttcct
      541 cttgataaga gaaaatatac cgttaaagaa cttttaagtg cgttagttgt taataacgcc
      601 aatagccccg ctattgcttt agctgaaaaa ataggcggaa ccgaacccaa atttgttgac
      661 aaaatgaaaa aacaattaag acaatggggc atttccgatg caaaggtcgt caattcaact
      721 ggcttaacta accatttttt aggagctaat acttatccta atacagaacc agatgatgaa
      781 aattgttttt gcgccactga tttagctatt attgccaggc atctcttatt agaatttcca
      841 gaagtactga aattatctag caaatcctcc actatttttg ctggacaaac catttacagt
      901 tataattaca tgcttaaagg catgccttgt tatcgagaag gcgtggatgg tctttttgtt
      961 ggttattcta aaaaagccgg tgcttctttt gtagctacta gtgtcgaaaa tcaaatgagg
     1021 gttattacag tagttttaaa tgctgatcaa agccacgagg atgatttagc tatatttaaa
     1081 acaaccaatc aattgttgca gtacctttta attaattttc aaaaagtcca gttaattgaa
     1141 aataataaac cagtaaaaac gttatatgtc ttagacagtc ctgaaaaaac tgtcaaactt
     1201 gtagcccaaa atagtttatt ttttatcaaa ccaatacata caaagaccaa aaataccgtc
     1261 catattacta agaaatcatc cacaatgatc gcacctctat caaagggaca agtcttaggt
     1321 agagcaaccc ttcaagataa acatcttatt ggacaaggtt atctggatac tcctccttct
     1381 atcaatctta tccttcaaaa aaacatttct aaaagtttct ttttaaaggt ctggtggaac
     1441 cgttttgtga ggtatgtcaa tacctcttta tagaataaca caatgctttt tgtatgacat
     1501 tgactgacag agtacttctg cagtcttttt catttttatt taagcaaact aggccaatcc
     1561 taacaatttt tcaaaaaaac ttcacattgt attagcaaag tgatatactg aaaatgcatt
     1621 acatttaaag gagtcggaaa ttgaccaaaa gaattatact aactattttt acctttattt
     1681 gcttttcggt gatgcctctt gtccacgccg aagatgtcat ggatattaca aggcaagcag
     1741 gctacacagt tagtgaggtt aatcgaccaa aatcttctat tgtcgttgat gcaaatagtt
     1801 cagatattct ttggcaagat aatattgata ttcctaggga tcctgctagt atgtctaaga
     1861 tgtttacgct ttacatttta tttgaagagc tcgctaaggg aaaaattacg atggacacta
     1921 ctattacggc aactccaacc gatcaagcta tcgctaacat ttatgagatt agcaataata
     1981 atatcgtcgc tggagtagct tatcctattc gtgatcttat tactatgaca gctgtgcctt
     2041 catcaaatgc agcaactgtt atgattgcca attatttatc aaacaatgat gcttctgcgt
     2101 ttatcgatcg tgttaatgcc acagccaaac aattaggcat gactaacact catttttcaa
     2161 acgctagtgg tgcagcagca caagcttttc aaggttatta taatcctact aaatatgatt
     2221 tatctgcttc aaatattacg accgctcgag atttgtccaa gttactctat gccttcttaa
     2281 aaaaataccc tgaaattatc tcctttacaa ataaatctgt tgtgcacact atggtaggaa
     2341 cgccatatga agaagaattt cacacctata accactctct cccagataat caatttggta
     2401 tgaaaggagt tgatggatta aaaacaggtt ctagtcccag tgctgctttt aacgccatga
     2461 taactgccaa aagaggcaaa actcgactca ttactattgt catgggggtc ggagattggt
     2521 cagatcaaaa cggggagttt tatagacatc ctttcgtcaa tgccttgact gagaaagggt
     2581 ttaaagatag taagacctta tccaaaaaag cacgccaaaa actcgaaaaa ttagtgccac
     2641 aaaccaaaaa agaaacatca tctaaacagc agcacttcaa agccactaaa aagcaatcgt
     2701 acttggaaag agtcgaagat tttatgaatc acaaccacac tttcttgctt atttgcttag
     2761 ctatctttat aattactatc cttttactaa gcctcgttgt ctttgcaatg ggacgccaat
     2821 aatgaattat caaaaacctc tgtcaaagca cagaggtttt tgatatgtta tcttacgtgt
     2881 tcaaggcaac taaaaactaa ccacttgggg ttagtactac aactcataaa atcctatctc
     2941 aaagatcttt cagatggata agagaaaatg caattaaccg acagaacctt ccatctcata
     3001 ggaaatcaat cgattgagtt cgaccgcgta ttccattggc aattctttgg taaaaggctc
     3061 cacaaatccc atgacaatca tctctgtggc ttcgctttct gataagccac ggctcatgag
     3121 atagtagagt tgttcttcag aaatcttaga cactttggct tcatgttcta acgccacctg
     3181 tgaattatga atttcgttaa atgggattgt atctgattta gaaatatcgt ccatcaaaat
     3241 agtatcacat tcaatatggg aaacggattt tttagattgc ttgttaaagg tcacttggcc
     3301 acgataatct actttaccac cagacttggc aattgatttt gacacaattg atgatgatgt
     3361 atgtggagca ttgtgaatca ttttagcccc tgtatcttgg tgttgacctg cattagcaaa
     3421 ggcaatagac agcatggtgc cacgcgcacc tggcccatca agataaacag aagggtactt
     3481 catggtggtt ttagctccta gattgccatc aatccattcc actgttgcat ccgtaagggc
     3541 gcgtgcacgt tttgttacta gattatacac attgtctgac cagttttgaa tggtggtata
     3601 acgcatgtat gcaccatcaa gcgcaaagat ttcaacgata gcagcatgta agctgttact
     3661 tgaataagtg ggagctgtac atccttcaac ataatgaaca cttgctcctt catcaacaat
     3721 aatcaatgtc cgctcaaatt gaccagtatt ttcattgtta atgcggaaat aagtttgcaa
     3781 agggatatct accttgaccc ctttaggaac ataaataaag gtaccaccag accaagctgc
     3841 tgaattaagg gctgctaatt tgttgtctgt tggcggaacc agtttggcaa agtattgttt
     3901 gaacaaatca ggatattctt tgagggcgga atcggtatct gtaaagataa tccctagctt
     3961 ttcaaattca cccttcatgt tatggtaaac cacttctgac tcatactgag ctgatgcccc
     4021 agcaagataa gcacgttctg cttctggaat tccaatacga tcaaaggttt cttttatttt
     4081 ttctggaaca tcatcccaag aacgagctgg cttatcagat gctttttgat agtaaatgat
     4141 atcatcaaag ttaatatctg ataagtctgc tccccaggtt tgcatcggca ttttattaaa
     4201 ggtttccaag gattttaaac gaaactccaa catccactca ggttcatttt tggcagctga
     4261 tagttcgcga accactgcct cactcaatcc ttttcctgta gaataaatgg gctctacatc
     4321 atcgtgaaat ccaaattggt agtcccctaa gtcaattggc tttggctcta ctttctcatt
     4381 tatatcagac atttttccct tcctttacat tttggtccgt gaggtgctga gcattggcac
     4441 ttcgtttaat ggcttccttg agagcattcc aagctagggt agagcatttg atacgctgtg
     4501 gaaattttgc aactcctgcc aacaattctg cttcacctag ctctttttga gcaggatttt
     4561 cctgtccttg taccatctct gaaaaaatat cagctaacgc gagtgcttct tctttacttt
     4621 taccaataac agcatctgtc atcatgcttg atgaagctgt ggaaatggta cagccgttgc
     4681 ctgcaaaagc aatatcttca attttatctt cgtcaaactt aacggtcaaa gaaatcacat
     4741 caccacaagt cggattattc agttgaacag cctctacgcc atctagttgc ccatgatgat
     4801 gtggacgttt cgaatggtcc gctaccacag ccatgtatag atggttcagt ttagagagtg
     4861 ccattgaaaa actcctttgc ttttagaatt gcttcgacta gacggtcaca atctgccttg
     4921 gtgttataga tataaaaact tgctctaaca gttgctggta cacctaaata actaagaaga
     4981 ggttgggcgc agtggtgccc tgctcttact gcaacacctt catagtccaa ggctgttgcc
     5041 aagtcatgag gatgcaaatc atccagatta aaagaaatca gaccagatct tgcactaggc
     5101 tggcttggtc catatatggt aagcccctca atagcttcta atttcggcaa gacataggct
     5161 attagttctg cttcatgcgc atgtatatca gccatgccta gtctctgaag gtaagaaatg
     5221 gctgcgctta gcccaatagc accagctatg tgaggtgttc ctgcttcaaa cttccagggc
     5281 aattctttcc aagtggcctc ttgttcgtaa acaaaatcaa tcatttctcc gccaaattca
     5341 agaggctcca cttgattcaa aagctcttct ttgccgtaaa gaacacccaa acctgttggc
     5401 cccaacatct tatgagctga aaaagcaaag aaatcacaat ccaagtcttg aacatcaata
     5461 gccaaatgtg gaaccgactg ggcaccgtca acaacaaggt aggctccttt agcatgtgcc
     5521 agcttggcaa tttctttaat gggattgatg caaccaagaa cattggagac atgtactagg
     5581 ctaacaaaac gtgtttttgt cgtcaatttg tttgccaaat cgtccatgtc aagttggcca
     5641 tcttttaaat aaacatagac taaccttgct cctgtttttt gacaggcttg ttgccaagga
     5701 atgatattgg catgatgctc cataatcgaa atcaacacct catcttctgg cgtcaagacc
     5761 tgctctgcaa accgagcaac ccaattcaaa ctggttgttg ttcctctggt aaagagcact
     5821 tccttacttg atttagcatg aataaagtca gcaacctgct ggcgactggc ctcatatttg
     5881 agcgttgcac gttcagccaa ggtatgaact cctcggtgga cattagcatt atcttcttga
     5941 taataggatt gcaaagcctc aagaacaagc gccggttttt gtgtggtggc ggcattatcc
     6001 aaataaacaa ggggttcatc attgacttgt tgatttaaga tttgaaagtc ttgtttaatg
     6061 tcttttgcgt ctagcaatac taacctcttt ctttttggca gtaggtctta acgattaagc
     6121 aatttctcat ctaaaacctt aataatctct tggcggactg atggaatagg aatttcagcg
     6181 ataaccgctc ctaggaatcc tctaataacc aatcgttctg ctgtttcttg atccagtcct
     6241 cgactcatca agtaatacat atcttcaggg tcaacctgac cgatagaagc tgcatgacct
     6301 gctgttactt cattttcatc aattaagagg attggattgg catcggctct tgcttggtca
     6361 gaaagcatca aaacacggct ttcttgttga gcatcagctc ccttagcgtc ttttagaata
     6421 tgaccaatcc cgttaaacgt taaggtgcca cgttccaaaa tcacaccatg ctgtaaaata
     6481 tgaccgaccg tacgttgacc atagttggtc acgcgcgtgt caataccttg tacctgacga
     6541 ccacttgagg ctgcaacaac tttcaaatca gcttgtgagc cctgaccaat caaatcactg
     6601 tcaaaatcag caatgacatt gccttcgttc atcacagcta aggcccaatc aatgttggca
     6661 tccttctcta aacgtcctcg acggctaata taggttgtca ctgaaggacc taagcggtcg
     6721 atagccgaga atttaatctg gctgcctgct tgagcaatca cttctacact gatattagcg
     6781 ctgatctttt gagtggcatt gccaatagat tcaaaacgct ctaaataggt gaacttactt
     6841 tcttttcctg caatcactag aacatgcttg ttaaaaggaa cgtcactgtc actatcttgt
     6901 aagaaaatag cttcaatagg agttgtgatt tccaagtgat caggaacgta gagcacggct
     6961 gcgctattaa aataagcagt gtggtaggca gctagtttgt cttcatcaaa agctaatgcc
     7021 tgaccaaaat gagcttcaat tacttctggg atttcctcaa gcgccgtata aaaatcactg
     7081 aaaacaactc ccttgtcaat taacgccatt ggtaactgtt ctaagactgt ttgcgtgcct
     7141 acctgaacaa gctttgggtt atctccaata gctataaaat ctggaacact agctagactt
     7201 tcattttctg ttaaggtacc atctcctaga ttccaacggt gaaatttaac cctttcgatg
     7261 gttggtaatt ccaaatttgg aatggcttct aatgccgcta aacgccgttc ttgcagccaa
     7321 gcaggctcag cgtgggcttg cgaaaaagcc actagttttt cttttgtcat ctgtcaatcc
     7381 tttgtctctg gtttttaaat tggctgggta tatagaacat cacctagctt tttgtagaaa
     7441 gctaaagtaa ttgtctccaa aaacttaact ttcttcctta tactcgattc ccagatcctg
     7501 tgcaatacct gcgtagcctt ctttttcaag acgagtagcc aaggcagcat caccagataa
     7561 gacaatacga ccatccatca taacatgaac taggtctggg gtaatgtaat tcaacaaacg
     7621 ttgatagtgg gtgataatca tagctccaaa gtctttccca cgcatctcat taaccccttt
     7681 agagactact tttagggcat caatatcaag accagaatca atctcatcta acaaagcaaa
     7741 tttaggctct aacatgagca attgaagaat ttcattgcgt tttttctccc caccagaaaa
     7801 accttcattg aggtaacgct ctgccatctc ttctttcata cctaacaaag ccattttctc
     7861 atcaagtttg gtgataaaat ctcgcacgga aatcttatcc tcatctgcct taccggcatt
     7921 cattgctgca cgcataaatt cagcgttggt aatccctgga atttctgaag ggtattgcat
     7981 ggcgagaaaa agtcccagtc tcgcacgctc atcaacttct agatcgagaa tattgacccc
     8041 gtcaagcaaa atttgccctt gggtcacttc gtagttagga tttcccataa tagctgctga
     8101 aagggtagac tttcctgtcc catttggccc cataatggca gctacttccc cagttttaag
     8161 agttaggtta acccctttca aaatctcttt accttctatt gaaacatgta aattattaat
     8221 ctcaagaatt gacatgatac accccttatt ttctctgtca tctataccat ctcaagaaaa
     8281 gacagtgtca tttgcactgc tttttttata atggtcactg catctattat agcaaaaaaa
     8341 gccttactcg gcttttttag tgtttcaaat ccttcttttc tttccatttc agaagcattg
     8401 cttgacgata atcactatta ccaataaact ttaacaagtt aaacaaaggc gttcgctttt
     8461 caccccagat ctccaatcct tcaataaaaa cttctaaacc aaataaaagt ccaagcatca
     8521 agaggacgcc accaattcga ctagaaacat ttaaaagtaa agaaataagg gaaaaaagca
     8581 tggtaatacc ataaactact aagacagctc ctcgatgagt aaagcccatt gataagagtc
     8641 gatgatgcag gtgcatctta tccggctcgt agaatttttg acctgataaa ctacgtcgaa
     8701 taatagccac gatggtatcc ataatgggta caccaagaat aataacaggt gtcaccacag
     8761 ccacagccgt tgaatttttc aatccttgaa gagacaaaac ccctatcata aagccaataa
     8821 agagggctcc tgtgtcaccc aaataaatca tagctggatg gtaattataa ggaaaaaatc
     8881 ctgcaatagc agaaatcaag accaaaatgg ttaacgtcaa aaagaaatcc ttttgaggta
     8941 aaaagaaata agatacaata gccatggtca ctaaactaat aatagagacc ccactaacca
     9001 aaccatccaa accgtcaatc aagttaatag cattagtgat ggaaacaatc cacaggactg
     9061 ttaagaataa ggttaagaaa ggaccaaaaa ctaacaacgg ccctccaaaa ggaattttga
     9121 agctatcaaa tttaaagtcg gtaaaagccc agacaatcac tgcaccaatt agaataccaa
     9181 acatctttaa tttaggactt aactcataca aatcatctaa aaaaccagta agggtaatta
     9241 ctgtagcacc aatcacgaca ggtaaaatat aactgaagta ggtctgacca aaaaacatgg
     9301 cgcctttaga agcaatcgga ataagtccca aacttgctac taaaaaagac ataaaaatag
     9361 ctaatccacc actggttggc atcggaacct tattgacccg tctggcattg ggattgtcta
     9421 ctgcacccac acgaaaggct aaaaagcgaa caagaggggt taagaataag gacattaaca
     9481 aagcccctat cagaacaagg acataatcta ttgtgaatga aaacatacta ttttatccca
     9541 actcaatctt ttgtaattgc tcaacagcac catcaagcat caattgtaag ccatgctctt
     9601 gtaaataagc tcttgttttt gatccagggt ttgcatgctc aatcaatctc gcatacatga
     9661 cgttcgcaaa ataagatggt tgttgctgaa catctaataa aatagtcata tggtagcagt
     9721 tgctcccctt atataactct gaagcttcta tcgaaaaatc aatcgtttta gcaaaagcca
     9781 ctgcctctgt aatcgttgaa aaatccaata cataatggac atagtctaaa ggttctgact
     9841 catgattagt tgatggatca gccgcctcag actgattagc tagagtggct tcaacaacat
     9901 cttccattat ctcttcaatt ttttccaatt tttcatgagc tttgacatct cccttttcac
     9961 gcatggattg ctccaagctc ttgaaaaaat cttccggagt catttgggac atatcaccaa
    10021 actctgctaa atcttcaaga ttaatatctt tatttatctc agatttggta acaaaaacat
    10081 ctagacggtc cttgcgtgga gtcactcgaa aactaagcat accactatct ttaaaattat
    10141 ctggaagatc gagttcgtcc atcacagaat agaaaaactc ttctgttttt tcttgaggaa
    10201 tcaagaaatc tttcaactcc attcctcttt cttctaaatc gtccatacta attgttattt
    10261 ttagcgttgt ctcgctaatt tgtttcattt ccattctcaa tacctcgcac gcgtagtcat
    10321 tttattatac tagaaatgct gtaatttcgc aatttatgac taactcctcg tctacaaaag
    10381 gaaatttaaa aagcgacagt ccccaaggtc tgtcgcaaca tcctaaacga tatgtaagtt
    10441 taaaaaacaa atttaaagaa gctataaata agtaaaaggc ttcctaacac aatacggtat
    10501 ttcccaaaaa tggtgaaatc gtgtttctta acataatctg tcaagaacct aatcgccaat
    10561 aaactaacca caaaagcggt caagctagcc actaaaagaa taaggacttg tgcaaaatca
    10621 aggtgatggc catcaaggaa aaattttaca gcctttaatc cactatatcc aaacatggta
    10681 ggaatggcca aaaagaaggt gaaatcagcg gccactgttc gactagttcc taaaataata
    10741 gctcctaaga tggtagcccc tgatcgacta gtaccaggca caatacttaa gacttgaaaa
    10801 caaccaatga agaaagctgt tttgtaagac attctagcta attcagtgac agcaggttct
    10861 tgttgagcat ttcgtttttc aatccagata aaggcaatcc cgtagacgat taaagcgatg
    10921 gcaatcggaa ccataaaata aaaatgtgct tcaaaccaat tatcaagagg aaccgctatt
    10981 aaaatagaag gaatacacgc aatcaccact tttagccata attgccaggt aagctgtact
    11041 tctcttgctg ttttcccagg ttgaaaggga ttgagacgtt caaaataaat caacataaca
    11101 gcgataatcg cccctaattg aataacgata ttaaacattt ctataaaagc cttatcctga
    11161 tttagtcgga taaattcctg caccaaaatg aggtgaccag tgcttgaaac tggtaaccat
    11221 tctgtgattc cctcaatgat gccaaagaaa atggctttta gcaattcgat aattaacatg
    11281 ttttctcctt attaaccact cttattgtga ttttctaaac tgtgattaat gccaagcagc
    11341 ttttttaaaa ggcaccgccg ccgccaccac cactaatccc gcctccggaa gagactgaga
    11401 aatttgagga agttgtagct gaagacaagc tagacacaaa ggttggtgtc gtagcataca
    11461 ttaccattga taattcacct ggtcgaacag cttggtaaac ctcaggtaag gcaatacgat
    11521 ggactttcaa atagcgttcc actttcttag cgtaaccaaa taaggtggca taaaccaaaa
    11581 cacggttcca aacaaccaaa ccttcaatag caacatcctc aaatttgtca atgtctcgta
    11641 tcatgttttt aaagctctgc cattgatgta accgaacacc cccctcaggt gtttcaatac
    11701 ccaatttctt gtattgatct aacttcaggc taatgtaaaa gccaaggcac atcgttagca
    11761 ccattaaagc aaggtaaatg agggctaacc cactttcttt aatagccaag taaatcaata
    11821 aaccactatt tagaataagc aacaaacaac ctaatccctg gacactattg ctttttcgtt
    11881 tctctgccgg ggtcatttga cgataagtat ctggtaagtt taactttttg atagtttcta
    11941 ggacattgtt tgtaatagct gccgacgctt ttttcatcgc cttaatcacc tgctcagacg
    12001 attgacggac ttcttgctcg agctttttac ctttgtacgt tttctttaat tgtttaatgg
    12061 tatcagcatc ataatggtat tgactaaaca attgatccac cggcaatgtc accttgtttc
    12121 caaaagccat atctaagaag gcagcttcct catctgtcac acgatctaac tgactgattt
    12181 caagataagc tttcccttct tctttcgtta ataaaagcac cttttggtcg atcagatcca
    12241 acaaggtagc ttgaacaagg gcctcaaagg tcactccttt agggatcaat aatttctggc
    12301 tttctgatgc tgtaggggat aaataggcaa aactctgtcc ataaatcgct tgtgtcaaca
    12361 caagaggcga aagatcttca gggacttcgt aactgtgatc tgtatggtta gccaaatgat
    12421 aacgattgaa ttgtttcttt aatcttgtaa actgtattaa ctgccaaaga agaaaaccta
    12481 cctctaccag aggaatgact ttgccaaata gcagctgtaa caaggtcctt cgtctggaaa
    12541 tcttagtttc taacgctaca attttattct ttttactcgt tgaaacatca actggctctt
    12601 ttcccaataa tgctttttta tcccaataag catgcaattc cagttgccct gagacattct
    12661 tggcattaat ctgatagcga ctgttacctt cttttaacac ttgaggtttc ttttggtaat
    12721 atcctctgtg agcccataga ttagaatcct gaatatcagt tggggtagtg acggtcaaac
    12781 tcaccttttc taatgttttg tcccaatcac taatcggtgt ccaattcagc tctgccacgt
    12841 cttgataagc tgttaaaagg tggtgcagtt gccagataac cttgacgtca accttatcac
    12901 cagcttggcc agcattataa agttttaaac gatagccatc tccaaggtca ctaaactctt
    12961 gactaacagg tacctgttga ccattctgat atacctcaac tttaggcttt tggtcaatag
    13021 caaagcccgc tggcaaatga cctgtccggc ccagcgaaat atactggccg ttataagaag
    13081 tgtcaaattg ataggttact ttttgctcaa accgagcagt attttctttt gataacagta
    13141 actgaccttc atagttggtg atgctatagt ctacatcagc tgcttttatt cgtatcccta
    13201 aaagactgaa gcagagcaca agcgtcatca aaattttttt catggcaaca tcctctttct
    13261 gccctcattt taccatattt taaggagatg acaaaaccta aaccaagtcc ttcatcttat
    13321 ttttaatcat tattttgaat atttttccat atttcaacct attcactgat aatatattga
    13381 aataggatgc aaattgctgt aaaataggtc tgaatactat ttttgaaaca ggagacctat
    13441 atgaaaaagc taatccttag ctgtttggtc gccttggccc ttctgtttgg aggaatgagc
    13501 cgggctcaag caaaccaata tttaagagtc ggaatggaag cagcctatgc tccttttaac
    13561 tggactcaag atgacgcttc aaacggggct gttccaattg aaggaactag ccaatacgcc
    13621 aacggttacg atgtccaagt cgctaaaaaa gtcgctaaag ctatgaacaa agaactttta
    13681 gtcgttaaga cctcttggac cggtttaatt ccagcattaa cttctggaaa aatcgatatg
    13741 atcgctgctg gtatgagtcc taccaaagag cgtagaaacg aaattagctt ctcaaacagc
    13801 tactacacta gccaacctgt tctagtcgta actgccaatg gcaaatatgc tgatgcaaca
    13861 agcctcaagg atttttctgg agctaaagta actgcccagc aaggcgtttg gcatgtcaat
    13921 ctcttaactc aactaaaagg tgctaagtta caaacaccaa tgggagattt ctctcaaatg
    13981 cgacaagccc ttacttcggg tgttatcgat gcctatattt ctgaacgacc tgaagccatg
    14041 actgctgaag ctgctgatag ccgtttgaaa atgatcactc ttaaaaaagg gtttgctgtt
    14101 gctgaatcag atgctgctat cgctgtcgga atgaaaaaaa atgacgatcg tatggcaact
    14161 gtcaaccaag tgcttgaagg attttctcaa acagatcgta tggccctgat ggatgatatg
    14221 gttaccaaac aacccgtgga aaagaaagcc gaagatgcta aagcatcatt tctaggccaa
    14281 atgtgggcta tttttaaagg taactggaag caattcttac gtggaactgg aatgaccctt
    14341 ctgatttcca tggtcggaac cattacaggt ctctttattg gattattaat cggtattttc
    14401 cgtacagctc ctaaagctaa gcataaagta gctgccttgg gacaaaaact ctttggttgg
    14461 ttactcacta tttatatcga aatcttccgt gggacaccta tgattgttca atctatggtt
    14521 atctactacg gaacagcgca agcctttggt atttcgatcg accgtaccct agcggctatt
    14581 tttatcgtat ctatcaatac gggtgcctat atgagtgaaa ttgttcgcgg tggtattttc
    14641 gctgtcgaca aaggtcaatt cgaagcagca actgctcttg gctttactca cggacaaacc
    14701 atgcgtaaaa tcgtgctacc acaagttgtt cgcaacattt taccagcaac aggtaatgag
    14761 tttgtcatca atatcaaaga tacttctgtc ttgaatgtta tctctgttgt ggaactttac
    14821 ttctcaggta ataccgtagc cacacaaacc taccaatatt tccaaacctt tacgattatc
    14881 gccattatct actttgttct taccttcacg gtgacacgta tccttcgtta tattgaacgc
    14941 cgtttcgatg ccgatactta caccactgga gcaaaccaaa tgcagattgc ggaggtctca
    15001 aatgtctaac tcaattattg aaatcaaaaa tctcaaaaaa tcttacggca gcaatgaagt
    15061 actcaaagat atctcccttt ctgttaacaa gggagaagtg atttccatta tcggttcttc
    15121 tggttctgga aaatcaaccc tcctgcgctc gatcaatctt ttggaagagc caagtgccgg
    15181 tcaaatcctc tttcatggtg aagatgtcct tgctgaacac tacaacttga ctcactaccg
    15241 tgaaaaacta ggcatggttt tccaatcctt taacttgttt gaaaacctaa atgtattgga
    15301 aaatgctatc gtcgctcaaa ccaccgtcct caaaagagac cgtgcacaag ccgaacaaat
    15361 cgctaaagaa aacctcaatg cggttggtat gacagaacaa tactggcaag ccaaaccaaa
    15421 acaattatca ggggggcaaa aacaacgggt tgccattgcc agagccttat ctgtcaatcc
    15481 agaagccatg ctctttgatg aaccaacatc agctcttgat cctgaaatgg ttggtgaggt
    15541 ccttaaaacc atgcaagact tggcaaaatc agggttgacc atgattattg ttacccatga
    15601 aatggaattt gctcgtgatg tttctgaccg tatcatcttc atggataagg gattgattac
    15661 tgaagaagga agtcctcaac aaattttcga gaatcctaca caagatcgta ccaaagaatt
    15721 tttacaacgc ttcttaaaat aagataaagg atccaactat ttctatgcca gttgggtctt
    15781 ttttcaattc aaaaagaatt tgtcataaaa tactttacca gttaactatt ttatgctata
    15841 cttatttttg ttaacgttaa taatttatat ttaggaggat aatatggcag aaaaaactga
    15901 cttatcgtca gcttacagaa gactgaaaag tcccaatatt aaaaccagaa aacgtgctct
    15961 gaagattatt cacgagtaca aacgttacgg taaaaaataa gattttactt tttatctctc
    16021 caaatttaag tcagacaaaa aaaggctaag acaccccttc catcttagcc tttttgtata
    16081 cagtttatta agggaactat tttaaaatat cttttatgct agagtctaga atagttcaaa
    16141 acgttaaaat catacattta aggtagtgtt taaagtatac gaaaaagaac tcagcataaa
    16201 ccaagctctc tttcgttata actaattatt tagcaatttt tgcgaagtac tcaagagtac
    16261 gtacaagttg agcagtgtaa gacatttcgt tgtcatacca tgatacaact ttaaccaatt
    16321 gtgatccgtc aacttccatt actttagttt gagttgcgtc aaacaatgaa ccgtatgata
    16381 cgcctacgat atctgaagaa acgattggat cttcagtgta accgaagcta tcgtttgaag
    16441 cagctttcat agcagcgttg atttcgtcaa cagaaacgtt tttgtcaaga gttacaacca
    16501 actcagttac tgatccagtt ggaacaggaa cacgttgtgc agcaccgtca agtttaccgt
    16561 taagttctgg gataacaaga ccgatagctt tagcagcacc agttgagtta ggaacgatat
    16621 ttgcagcacc agcgcgtgca cgacgaaggt caccaccacg gtgtggtccg tcaaggatca
    16681 tttggtcacc agtgtaagcg tggattgtag tcataagacc tttttgaata ccgaatgcat
    16741 cgtgaagagc tttagccata ggagctaaac agtttgtagt acatgaagca cctgagataa
    16801 ctgtttcagt accgtcaaga atgtcgtggt tagtgttgaa aacaactgtt ttaacatcgt
    16861 ttccaccagg agctgtgata acaacttttt tagcaccgtt agcatgtaag tgtttttcag
    16921 ctgcttcttt tttagcaaag aaaccagttg cttcaagaac gatttcaacc ccatcagttg
    16981 cccagtcgat gttttctgga tcacgttcag cagaaacttt gatgaagttt ccgtttactt
    17041 caaatccacc ttctttaact tcaactgttc catcaaaacg accttgagtt gtatcgtatt
    17101 tcaacaagtg tgcaagcata tttggatctg taaggtcatt gatacgagtt acttcaacac
    17161 cttcgatgtt ttgaatacgg cggaatgcaa gacgtccgat acgaccgaaa ccgttaatac
    17221 caactttaac taccattagt gatttcctcc ttatgaaaat caaaagaata ttatttagag
    17281 ggaatgctcc ctacctattg tgaaaagatt aacttacagt catctgtaac aacctttcaa
    17341 cagaactatt atataactat ttcagaaaaa atgcaaataa attagccatt ttcataaagg
    17401 gcagcttctt tcttctagcc ttcaaaaccc accaaatata ggaaagggct ttcttttttc
    17461 tatgttctat tctgtaattt atctttttat tgggaaagat ttccaaaact tcaaactgta
    17521 ctataacgtt ctgttacact aaaaaagagg ttaggatagt tatcctcaac ctcttagtgg
    17581 tggttattca cctttgtttt tcttaatgat ttcttcttga actgacttag gaacatcttc
    17641 atagtggtca aataccatca tgaatgtacc acgtccttga gtagcagaac gaagaactgt
    17701 tgcataaccg aacatttcag caagtggaac ataagcacga acgatttggc tgttaccatg
    17761 tgcttccata ccatcaacgc gtccacgacg agctgtcacg tgtcccataa cgtctccaag
    17821 attgtcttct ggagctgtaa tcgttacaag catcatcggc tcaaggatag ctggttgtgc
    17881 tgatttagct gcttctttaa gagcaagaga tgctgcgatc ttgaaggcag tttcagatga
    17941 gtcgacatcg tggtatgaac catcgtaaag tttagcttta acgtcaacca ttgggtaacc
    18001 agcaagaaca ccgtttgcca tagattcgat caaccctttt tcaacagctg ggataaattc
    18061 acgaggaacc acaccaccaa cgatagcatt ttcgaattcg aaacctttac cttcttcatt
    18121 tggtgtaaat tcaatccaaa catcaccgaa ttgaccttta ccaccagatt ggcgtttgaa
    18181 gaatccacga gcttgtgtag aagcacggaa tgtttcacgg taagatactt gaggagcacc
    18241 aacgttagct tcaactttga actcacgttt catacggtca acaaggacat caaggtgaag
    18301 ttcacccata ccagcgataa ctgtttcacc agtttcaacg tttgtttcaa cacggaatgt
    18361 tggatcttct tcagcaagtt tttgaagggc aacacccatc ttgtcttggt ctgctttaga
    18421 ttttggctca accatcaact ggataactgg ttctggaact tcgattgact caagaataac
    18481 ttttgctttt tcatcagtca atgagtcacc agttgttgtg tctttcaaac caacagcggc
    18541 agcgatgtca ccagcgtaaa ctgtttcaat ttcttgacgg ctgtttgcgt gcatttgaag
    18601 gatacgtccg atacgctcac gtttaccttt tgatgtgttc ataacgtaag aaccgctatt
    18661 aagaacacct gagtaaacac ggaagaatgt caaacgacct acgaatgggt cagtcatgat
    18721 cttgaaggca agagctgcaa atggctcttc atcagatgct ggacgttctt cttcagcatc
    18781 tgtatccggg ttaacacctt tgattgcagg gatgtcaagt gggcttggaa ggtatgcgat
    18841 aaccgcatca agcatcaatt gaacaccttt gtttttgaag gcagaaccac aaagtactgg
    18901 gaagaattca acgttgattg ttgctttacg gataccagca atcaattcgt cgttagtgat
    18961 ttcttcacct tcaaggtatt tcatcatcaa gtcttcatca gtttcagcaa ctgcttcgat
    19021 taatttttca cggtattctt gagcttgttc taagtattct tctggaatat cttcttcaag
    19081 aatatctgta ccaaggtcgt tggtatagat ttctgctttc atcttgatca agtcaatgat
    19141 accacggaaa tcatcttctg caccgattgg caattggatt gggtgtgcat ttgcttgaag
    19201 acggtcatga agggtttgta ctgagtaaag gaagtcagca ccgattttat ccattttgtt
    19261 ggcgaaaacg atacgtggaa caccgtattc agttgcttga cgccaaactg tttcagtttg
    19321 aggctcaaca cccgattgtg agtcaagaac ggttacagca ccatcaagaa cacggagaga
    19381 acgttgtact tcgatggtga agtccacgtg tcctggtgtg tcgatgatgt taacgcggtg
    19441 accatcccat tgagctgttg tagcggcaga agtaatagtg ataccacgtt cttgctcttg
    19501 ctccatccag tccatttgtg atgcgccttc gtgcgtttca ccgattttat gaattttacc
    19561 agtgtagtaa aggatacgct ctgttgtagt tgttttacca gcatcgacgt gagccatgat
    19621 accgatatta cgagtttttg caagtgaaaa ttcgcgagcc atgtgtttct cctatttttt
    19681 tagtttactc tttcattata gcactattta gcaaaaacgg ataggcagga cctacccgtt
    19741 atctaaatat ttgttggtaa agataatacc tattgccttt ataaggcttg gtatcttatt
    19801 cattgaactc aggctaaacg ctgtgtgaaa aagataaact ttcctagaga caaaggtttc
    19861 ttcgtcaaat ttcctatttt cactttgcgt tctatacgcc ttcgtatctt attaattgag
    19921 ttcgagctac aagccactga caaaaagata atagttccta gaaactgaag tttctgcgtc
    19981 aatttcccta ttttgtctag catttgtttg acgctaaaat atcttatatt accaacggaa
    20041 gtgagcaaag gcacggttag cttcagccat tttgtgagtg tcttcacgtt tcttaactga
    20101 tgcacctgtg ttgtttgcag catccatgat ttctttagca agacgatctt tcatagtgtg
    20161 ttcaccacga gcacgtgatg cgtttaccaa ccaacgaagt ccaagtgttg tacgacgttc
    20221 tggacgaact tcaactggga cttggtagtt agaaccaccg acacggcgtg cgcgtacttc
    20281 aagtacaggc atgatgttgt ccatagctgt ttcaaatact tcaagagcgt catttcctgt
    20341 tgcttctttg atagcgttga aagcatcgta aacgatagta gcagctgtac cacgtttacc
    20401 gtcaagcata acacggttga taagacgtgt tacgattttt gagttatata atggatctgg
    20461 taatacttcg cgtttaggcg cttgattttt acgactcatt ttctcttatc ccccttctat
    20521 tatccttttg gacgtttcgc accgtattta gaacggcctt gtttacggtc agcaacacct
    20581 gcagtatcaa gtgcgccacg aacgatatgg taacgtaccc ctggaaggtc ttttacacgt
    20641 ccgccacgga taagaacaac gctgtgctct tgcaagttgt gtccgatacc tgggatgtag
    20701 gcagttactt cgataaggtt gctcaaacgt acacgagcga atttacgaag ggctgagtta
    20761 ggttttttag gtgtcattgt tccaacacga gtagcaacgc cacgtttttg tggggcagcc
    20821 attttagttt gaactttttt gtgactgttg taaccaatgt tcaaagctgg tgaatctgat
    20881 ttttcaattt tagatttacg tggtttacgt accaactggt taattgtagg catctacatt
    20941 ctcctgtgtt tttttatttt tggttgatat agcacttggt gacagcccat atccggtgca
    21001 ctttttgcaa caattgtcag cacgtctctg tacacttttg agagaccaaa agtaaaaagt
    21061 accgtctaat ataataacac agacggcact tcatgtcaaa tagaattttt cctttgatgc
    21121 aattgcttat gacgaatgca tcacttagat tccttacgga acctaaatcc tgtgaggccg
    21181 acagctgcca acatgacgac tcctgcttta agagctcttt gagtactttc atcaacatca
    21241 gaaatgagat tgctcgttgt agaagatcct gagccataag atgtcttaga tgagggctga
    21301 gtaatcgaag atgtgatttt agacacaatg gcattacttg ctaccatttc agaggtttgg
    21361 ccaacaagcg cttctgctac aagacttgta ttttcagcag ctaatcttgc tgaagcttct
    21421 aatagttgtt tcgtttcttt aaccatttct tgaacaagcg gagtagtatc tatcttacta
    21481 tatgatagcg gttttacgcc cgtaagaggt ggagctactt gcaaatcagg cacagtagct
    21541 atatcttcgt ctaagtggcg atattccttt tcgttagcta aggttttaag caaagtcaac
    21601 tggtgttctg tggtagcaat agtagcttct agactgcttt gtttagcttg taaggctgct
    21661 aaagcttctt gtgcatttaa caaagatgag gtagttttag ccaaatcttg cttagtatta
    21721 tcaatgcgct cacgtatcac ttgaaggcgg ttaggattca atttaaagtc ccttagatat
    21781 tgcaaatgag cttttttagc caccagtgct gtcactctgg ctgcggcttg ctctgctaag
    21841 gcttctgtct ggtgcagtgc ggctttcaac gatgctagct tagctaatga ttgatcacga
    21901 gtagcttcga gttgtgcttg ttttgcttta gctgctagta attctgttct caaagaacct
    21961 ttagtatcat ttaattgtct cacttggaga tttaagctgt ctgactgctt aagtgtgctt
    22021 gctaatttac cttgaagttg acttacttta gcttgggctg ccataatatc agcttcttga
    22081 tgaatagccg acaaacgatt ttctaatgag cttacttttc ctttgatcgc tgcaatagta
    22141 tcagatacag tgcctactct ttgggcataa tcttttgtac ttccaacggc ttttataggg
    22201 gtcttattaa agcgttgatg gttagcaatg ttagactctg gaaacattac aaagtgttca
    22261 ttcaaagatc ctacattgct ggttgaaaat ccaaggtaaa caggcgcatt agggttatgt
    22321 ttatctacac gtaaaaagtt aatagcatgg ccgtatgtat ttccgtgtaa atgatctgta
    22381 aagagcatat acttgatact gtcataaata ccacgtttaa taccattcac agtatgcaca
    22441 tcgttaaaag caccgatatt ctcgtacatg ttatcatcat ttcgaatgag ccctgacgct
    22501 ccggcagagt cttcaataat agttttatca tgaggcccaa caccataatg ccctgatacc
    22561 cctggctgtc cgtagacaaa tgatggtctt gtattaccat gagttttctt atagctggta
    22621 ctaagtaatc ttgcaaattc ttgtgatcct gctgtaacag taactggtgg tagacctaat
    22681 tgtcttctta cactattaat catgtgagct gcaaactgcg ctagctcatt ttgcacttct
    22741 ggtgtcaaat tatcgggatc aacaaagcga ttacgatctg ctggaatatc ttggtattga
    22801 tttaattgat tacctggact agctttggca ataatttgat ctgcatgctc tttgtaataa
    22861 ttattgtaac tagctgatcc aatataacca ctagcttcta attttttaag ttcttcaaga
    22921 ggatagcctt gcggtgcttt catggtatta ttacccacaa tgctatcttg agtagacgga
    22981 gctgaggatt taagacgact aagttctgcc tctttttctg ctagagcagc tttctgagct
    23041 gccaactctt cagtcaattg ctttttaact ttagcttttt gattttctaa gtcagcttta
    23101 gccttctcca attctgagct taatgctttt gtattatcat tagccgtttg agctgcttta
    23161 gtgatagcat caggattgct aatcatagca ttgagcttag caatattttg ttcagacgtt
    23221 ttgacttgtt ccactaaatc ttgagctcga gtagtttctg ctgaaatgct agctttttgt
    23281 tctgacaatg cagtctcttt tgaatgttga tctgcttgag cattatgaag ctctgtttca
    23341 gtagctgtta actctctttg atgttcggct ccttgggcta atagcgtctc ctcactactt
    23401 gcaagagtat tagtgtaaat ttcttgtgca gaggttaaag ctttttgttc attatcttgc
    23461 tgctctttta agtggttgat ttcagcagta gtttttgtca gagcggtagc aagctctgtc
    23521 agttctgctt tttgttgact gagagttttt tcaactgcat caatagttgc ctttgcctct
    23581 tgaattgttt ctggttttgg taaactatcg tcgtgagtat tactcgcctt cgtttctcct
    23641 gaggctctat catctgcttt gacttggtga gatacgccta cactggcact caataaagca
    23701 attgttgagg ttaaagcaat tttctgctta acttggtttg gcttcgtttg ttctaagtcc
    23761 attctctctc ctttttccat ttatcgaaaa tattaccaac ttctacaaca acattgttgt
    23821 ttttgacatc aatctctgaa actttcagca atgatttgaa ggtcatttgc tcacgctctg
    23881 attgagcgtt ttctgcaaac acagcaacac caactaaagt gctgtcaaat tctgtcaaga
    23941 gactgatcat tcctgtaatc gtccccccgc ccttcaagaa atcatctaca atcaatacac
    24001 gactattggg tttcaaacta cgctttgaca gaaacatctt ttcaatacga tcactagaag
    24061 cactcgcata attgacagac actgtcgatc cttctgttat ttttaaatca cgtctaacaa
    24121 tgacaaaagg aacacttaaa atattagcca ctgcatttgc taaaggaact cctttggttg
    24181 caactgtcat cacagcgtca atcttttctc ctttaaaggc attggcaata atacgaccaa
    24241 tattttgtaa aattttaggt gtactcagca agtctgacaa atagatatag cctccaggta
    24301 agatgcgatc actctctgat aacctttgac acaggtcttc aacaatggta cgcgcttctg
    24361 tttctgaaat actgggggta aaaattacgc ctccacttgc tcctgtaagt gtatcgatat
    24421 caccaatgtt agcttcttca aaagctttct tgataatagc gatatcttct gagatagaag
    24481 attttgcggc ctcatattta gttgcaaagg tgtttaaact agttaattta tacggattgt
    24541 taatcaaata atttgaaatg acaaccatac gttcacttcg tcttaatttc atagtttccc
    24601 catttttatg tactttttgc tattatacca taaagttaat aaaaaacgaa cattcgatta
    24661 ggaaaatatt ggtttttttg ttcttattaa tatcatgcaa gaattcaatc taagataaac
    24721 taatcaacat tcctactgaa aggacattct acttaataac cataacaaga gccaaaaaca
    24781 cttatgaaac catcataagt gttaagcaca tcaaaaatca tcacaaaagc aaaggaacta
    24841 ttactcgaga aacactgatc aatagtttgg cttgtaaaaa gaccgattat ccatggcaaa
    24901 aatccgattg gtcatctcgc cctcgctaac acggctaaga gctgttgtca tcatcatcat
    24961 attagcatca atattgtcaa tcatatgaat aatttcagct tccatgatgc gtggacgtac
    25021 cggactaccg tattctaatt gcccgtgatg gcttaaaatc acgtgcctaa ggacaatgac
    25081 ttcttctttg gtatcatcga tttgtagttc tgaaattact tttgtaattt cttcgttaat
    25141 gagagaaata tgcccaatca agttaccacg aacggtgtat tctgtattat ctggtcctgt
    25201 taattctatg actttagcta agtcatgtaa cataatacct gcaaacagta gactcttatt
    25261 taagtcaggg tagatatctc caatgctatc tgctagtctg accatcgtag cagtatggta
    25321 ggcgaggcct gactcaaaag catggtggtt tgttttagct gcaggataag tataaaattc
    25381 tttatcatac ttacgataaa gagcccttac aattcgttgc cacgttgcat tttcaatttt
    25441 aaagagcatc tgttccaaat agtcacgtac ttctgtcaca ctgacaggtg ccttttcttt
    25501 gaagtccttt ggatcattgg gctcacctgg gcgaacattt cgcaaggtga tttgattaac
    25561 ctgaggggta ccattataca cctcgcgtcg ccctttcata aacacgactt taccagcagt
    25621 aaattcttca acgttataag gttgggcatc ccacagattt cctgaaatct cgccagtgtc
    25681 atcctgaaag gtaaggctaa taaaatcttt accagctctt gtcttacgaa cctctgccga
    25741 cttaatgagg tagaaccctt caaaaagctg gtcttttttc atttgattaa ttttcattat
    25801 tttcctcttc taataagggc atgttcaaga gagattttgt tgcttggtct tgataagttt
    25861 caacggtatt caaggctcga acaatggcat ttgtccttgt tgaaatgagc tgatccagcg
    25921 tattattagc tgtattcatt tgtttttgag ccttggcaag caggccgcca aatttatcga
    25981 attctaactt gacattgcct aaaattttac tgatgtcatc agcatttttt tggatattaa
    26041 gggtcttgaa accaacagat aaggaattca gcaaagcaga cagggtcgaa gggcctgcaa
    26101 ccacaatatt ttcttcccga cgaaggctat caaagaaaga cgcatttctg accacttctg
    26161 aataaagacc ttctgttggt aagaacataa ctccgaaatt ggtcgtctct ggggggttca
    26221 agtacttttt atgaatgtct ttggcaaagc gtttgatagc tgccagaagt gcttttcggc
    26281 tagcctcgat ggccagttta tcaccaactt cgtaagcatc ttctaatcgg taataatctt
    26341 caagagggaa ttttgagtca atcggtaggt aaatataacc gccttgacca tttcctggga
    26401 gcttaatcgc atattctacg cgttcactag aaccactaac cgttacaaat tctctttcgt
    26461 actggcttga tgtcatgata tcctcaatga tttggcctag ttgaagttcg cctaaaatgc
    26521 ctcgtgtttt ggtattggac aaaaccttat ttaaagtacc cacatcttga gccacgctac
    26581 gcatttctcc caagccttta ttgacacttt ctagttgctt ggatacagaa tcgaaagagg
    26641 cgtgcagacg attttttaag gtttcttcca atttttcttc aacgatctgg cgcattttct
    26701 ccaaacgttt ttcgttagat tcttgcatat ttttgagcga ttggttgacc tgctggttaa
    26761 ttttttctaa gcgtttgtca gaccggtccc tactatcaga caaactacgg tgcaagacgt
    26821 cacgaatatc tgttaattgt tggtaaaggc ctgcttgttg tcggttcatc agctgtgtta
    26881 gctctaacaa ttgttgcttg ttagctgtat ccaactggta ggtcatttgg tcagacaaat
    26941 tatccgcgtt gccttctagg gtttgggcaa gctgatgttg aaggccgttg actttgaaca
    27001 acagataagc ccctaaacct aagagaacca agaccaaaag gaacaagata aggtccatac
    27061 taactccaat ctttactttg aataataatc aagtagccac ttggcaaact aactgtaata
    27121 ggttgatgga taaaggcatt actggaatag attttctttt tgaaaaaatt atcttgcgtc
    27181 aactcaaatt tagcaccagt aatggtcaag tcagcttctc cttctgccat aaatgccaca
    27241 taggtcatcc cttcttcttg atggataagg tgctgacctg ccgggcggta ggtaatcata
    27301 ttttgttggt cacgcaaggc gatttgtgcc ataaagggag caataccagg gtcgctaggc
    27361 aagaaaatat tggataagag gtggtccatt cgacccccaa aagccccaaa aatggtcact
    27421 tccgcctcag gaaaacgagc aaagacctct ttaagggcta attccgtatc cgtatcattt
    27481 ttttctggat gagcagtaat aaaaagctct gccttctctt ttatatccgt aaacgctttt
    27541 tgggaaaccg aatcaaaatc accaacagcc atattcaaag gaagcccatt ttctaataaa
    27601 aataggctcc ctctatcaat accgacaaaa taatcaaaat cacggctgat ataagagaga
    27661 tctcctcccg caaaaagagc aactttagac attcaaggcc gtccttaagg tttggacttg
    27721 gctcactagg tcactggcct tgaaaaggta agaacctgcc acaaagacat tggcccctgc
    27781 ttcataacaa gcacgaatgg tcttattgtc tactccgcca tcaacctcaa tgtcaaagga
    27841 caatcctttt tcatctcgcc atttggctac ggtagccact ttttctaaac attctgggat
    27901 aaaggcctga cccccaaaac ctgggttgac tgtcataatg aggacttggt caactaagtc
    27961 tagcaaaggt tctagggctg ttgcaggagt tcctggatta atcactaccc ctgctttcat
    28021 gccagctgcc ttgatttttt gaagggctcc atgaatatga cgagtacttt ctgtgtgaat
    28081 ggtcatgata tcagcaccag cttgtgcaaa agcctcaaca tagcgttcag gatcaaccac
    28141 cattagatgg caatcaaaga ccaatttact gtgctttctc atacttgcca caacatctgc
    28201 accaaaactg atgttaggaa caaattgacc atccataata tcaatgtgaa cgtattcggc
    28261 atctgtttct tcaatacgag ctagctcaga agcaaaatta gcataatcag ctgctagaat
    28321 agatggggca atttttagag ttgacatgga gtagacctac tttcttttga taactttttt
    28381 atatgtttca cgacggtttt caatttcact gagaaattgc aggtaatgct cataacgaac
    28441 tggccacaac tctcccgttt caagcgctgc cttgacagca cacttgggtt cgtgggtatg
    28501 ggtacaggat ctaaacttac actcatggct cagccggcgc agctctggaa aagcctcgtt
    28561 caaatcttca gcattagcaa tatcatagtc taatgatgaa aaacctggcg tatccgcgat
    28621 ttttccgcca tgggtattgt aaaaactcac agctcgggtg gtgtgacgac cacgccctag
    28681 actatctgag atttctccta tttcaagagc caattcgggg gcgattcggt tgagaagggt
    28741 tgatttccca acacctgttt gtcccataaa gacggtaatt ttatcagcta gcaaaggcag
    28801 caattcctca agactggtca caaaatcgta accgattgcc tgatactgtc gaccaatagc
    28861 cttaatctcc tcgggactat ctaataagtc catcttagag atataaacca ctggatgaat
    28921 ggctttgtgt tcaagcaaga tgagaaagcg gtctagaaga ttgctgttaa attctggttc
    28981 tttagcagac ataataacca ctgcttgatc aatattgacg ataggaggtc ttaccaaact
    29041 gtttttacga ggatgaatcg ctaaaatata accttcagag ttgtcctctg ctgagaaatc
    29101 cacgatatct ccaacataag gtgtctcacc gcgcttccta aaattaccac gcgctcgcgt
    29161 ctgatatacc tgtccttctg actccacgta ataaaaacca gccagagatt taatgatttt
    29221 accttgcaac tgtgctcctt tgccctacta taataactct attatatcag aaaaccgctc
    29281 ccaaatcagc atttgagtct aagtttcact cgctttaaaa actgtcaaga ttctctagac
    29341 cactggctat catttaggtc actgcttttt aaaaatagaa tattttatca actaaaaatc
    29401 agatcactga tgattttttg aggtaggaaa cgatatgaat tttaagaaga cctttgctat
    29461 ttacgaaaaa gagtctaacc gtaccgctat agtcacgctc ttgttaattg cccttttttt
    29521 ccggggtatt aaaaaaacta gctctgcact gcgatgcctt aacggccagc ttcaataaga
    29581 aaaatccccc tgaactgaac atctctgttt tgtctacaag ggaatttttt tattgtcacc
    29641 gggggaaagc ttttataatc caacttcttt taaagcatca gctaatttgc caaaatcttg
    29701 aatcgacaag gcctcaccac gaatagaagg cttgatatct gctagtgcca gacctttttc
    29761 aagtttagcc ttgatgtctt cagacttccc aaaatggcta gtcaagttat tccacagggt
    29821 cttgcgacga tggacaaaac tgagacgaga cactctgaaa aagaaatctt cgtccttaac
    29881 ctcaatcaaa ggctggtcgc gacgcaccat tttgaggata gcactatcga catttggtgc
    29941 aggtacaaag acggtgcgcg gcacaatgaa ggcaaccttg gcagtcatgt aatactgcac
    30001 cgcaatggac aaggaaccat aagctttagt gtttggctcc gcagaaatac gatctgccac
    30061 ttcacgctgc atcatgacca caaactcttg gaatggaatt ttgctctcaa tcaagtgcat
    30121 gagaataggc gttgtgatgt agtaaggtag attagccacc accttaatgg gcaaatcagg
    30181 atttttaaac tgcttgattt gggtctgcaa atcagctttt aaaatatctt gattgaccac
    30241 ttgcacatta tcaaaatcgc gcaaggtatc tgccaaaatc ggcaccaagc ggtcatcaat
    30301 ctcaaaagcc atcacttcag cagcattttc tgccaaaaat tccgtcaaag ccccaattcc
    30361 aggaccaatc tcgataacat tgacattctg atcaatctca gccgtgtcaa cgattttctg
    30421 taaaatattg gtatcggtca aaaagttctg accaaaagat ttcttaaagg taaatccgtg
    30481 acgatcaagg acagccttgg tcacgctata atctgcaatt ctcatatatt ctcttctttt
    30541 tatgatcact ttctttttgg catttgaacc actagcctaa acttttttgc caggacacgc
    30601 cattcagtta atggggctgt tatattttat tgtactcttt actcctcata tgtctccatc
    30661 acttcttcca cttctgctag ggtaatgcca aataattcga ggcgcttgag cagttgtttg
    30721 ccgttggcat aaccgatgcg gagcttttca cccaagtatt ccctgcgttt acggctatct
    30781 gacgccatta aaagccctag acgaatcagg tctgtttgac gaatgtcaaa ataagactca
    30841 tcatcatatt gctgagtgac atgagcaagt gccttttgga gatcctcaaa attggcatgt
    30901 tcaaccccta aggaacgacc cttactctta gaactcggaa ccgcctcgtt tcggtttaaa
    30961 aaggcatgac gagctgttgg cacagcagcc ataatgagct tacgaatgcg ctcaccatta
    31021 taatctgggt ctgttaaaac gataacacca cgcaaatcgt ttaggcgatt gattcgttct
    31081 aaatcttctt cagtgatagc agagcctcta gtctcatagg tatccacctc gtaaaaacgg
    31141 cgtaggttag ccgtgtcgtc cttgccttct acaacaagaa cctcttgaat attaattttt
    31201 tcagtcaagc ttgaataccc tctttgcatt agcagtcgtt gcttttgcaa cttcttccac
    31261 agtcatcccc cgtagctctg cgattttatc aaccacatag cgggtataag ctgtatgatt
    31321 ttgtttgcca cgttttggaa ctggtgtaag ataaggggca tctgtttcca ccaaaatctt
    31381 atccaaaggc aagtgctgcg ctgcctcttg aatatcaaga gcctttttga aagtgaccac
    31441 acctgaaaag gaaatcatca tgccaagttc aatgaaacgt tcagccatct ctaatgaccc
    31501 tgagtaagaa tgcatgatgc ctccacgagg gccaacacca gctgctttga tgacctcata
    31561 ggtatcttcc aaggcatcgc gggtgtgcac cacaaaaggc aaatcatggt cttttgctaa
    31621 ttgcatttgg cgcttaaaaa cctctatttg cacctctttg gggtcctcca tccaataata
    31681 gtctaagcca atttcaccta aggcaatcac tttggaatgt gataattgag agacgatagc
    31741 ctcctcaaca gcctctgaat aagaacctgc ttctgttgga tgccacccaa tagttgcata
    31801 aatattggca tatttattgg ctagagttag tgccccacta atagtggcct ggtcaaagcc
    31861 aaccacatta tggtaggcca cgcccatttc ttgagccaaa gtcaattctt cagtttcgtg
    31921 tccttgaaat tctgctacat tcaggtgcgt atgggtatca aaaatagtta gtttttctac
    31981 tttatttgtc atgatgttat tataacataa agatcttaac cacatcagaa aacttggtga
    32041 gaacttctgc cgtttaggtt gatcgtaaaa ccctttgcta gctgacactt gtctattttc
    32101 tacttttttg acaaatgacc tttaatagtc ggctcctaca taagccctca aaaatactac
    32161 aacctactca aataaagtgg gttgaacaaa tctgtatcac aaaaagggtc gctaccttct
    32221 gttagcgatc tatgctatac tagtaggtag ttaaactgta aggagtgtgc tttgctatgc
    32281 ctaaaaacca ccgcctaatt accaaaatcg aagcatcatt ggaacatatg accagtttag
    32341 aaaaagggat tgctcatttt tttattacaa ccgatctgac acctcaagag ttgaccgcat
    32401 ccgagatagt taagcgacta cacatctctc aagccgcctt gactcgattt gcaaaaaaat
    32461 gtggttttac tggttatcgt gcttttgcct ttgattacct tcacagtctt caagaatcac
    32521 aagagacttt ccaatctatt cacttagaat tgacaaagcg cgtgctgatg gactacgacg
    32581 ctcttattaa taaaacctat gagctcgtta atgaggaaaa attactgaac ttagccaagc
    32641 tgatcgattc ttctgagcga gtctatttct ttggcaaagg atcttctgga ctagtagcaa
    32701 gagaaatgaa gctacgcttt atgcgacttg gtttaatctg tgatgcttat tcagacactg
    32761 atggttttac ttgggcaaat agcctcgtca atgaaaattg ccttgttttt ggtttttctt
    32821 tatctggcaa aacaaactct gtcatcacag cacttcatca agctagtcag cgaggagcca
    32881 aaactgtcct cttaacaaca gataatcaaa ccgaatttga cgactcctta gatattattc
    32941 ccgtatcatc aactcaccaa ctacattatg ggaatcgtgt gtcaccacaa tttcctcttc
    33001 ttattatgat ggacattatt tatgcttacg ttttagcgat tgacaaaccg cacaaagaaa
    33061 aaatcttcaa aaacactatt attgacaaag aaaataacga cctttagact acaatagcaa
    33121 aaagggcgtt gttttctttt taaagggtcg tcattcacct cccccagtat acttaaacaa
    33181 gaactcttca cgtcatatag actcacagcc actaagatta gctactcgct aaattatttt
    33241 cgattttctc aagagttata aatgaacaag atcgctcatt cttttgttta aaatggtagt
    33301 aagctcctaa tataccagca ttatttccat gactagcaaa cttcagctga gttttcttgg
    33361 caagactaga aaccaagtag ctatcaagag ctgtctttaa tttatcagct aaatagtctt
    33421 tttgcgccat gataccgcca ccaagcacca ccacatttgg gttaacaacg tagcagatat
    33481 tagcaatacc ttgtcctaag taatccacct gcttactaat ggctgctata caaatcgcat
    33541 cacccgcttt agcctgctca aaaatacgac gaccatccca ttggctaatg tcatcgccat
    33601 aagctaagac cacctcttga acaagagctg tggtagatgc cagatcctga aactgaccat
    33661 ctgacaaatg aaggtaacca acttcgcaag ctgaatgact actaccatga aaaacttggg
    33721 aattaaacaa taaacagcca ccgattcccg tcccaattgt caagcaaaga gccacaggat
    33781 aatccttggc actaccagaa atagcttcgg ccagaccagc acagttaaca tcgttttcca
    33841 cttcacacgg taacccgaaa gtctcttcaa tctctttttt aaactgagtt ccagcatagt
    33901 taggaatctg agggccggca taaaagattt ctccctcatc aggattaacc attcctgcag
    33961 aagaaatggc cactccagcc agatccattt ggtcttgata ggtctttacc aagcccttga
    34021 ccttttccaa aatagaggga ccacctttat aagcctcagt ggccatttcc tctttttcca
    34081 acaggtctcc tgtttcagaa atcaagccat acttgatagc agtaccacca atatcaattg
    34141 ctaaataatg cttcatttgt tatcccctcg catcttatca aaaggtttcc ttagcctgat
    34201 taatcagagc agcagcccgt tggcaaataa ccctatcttc ctcgaccaac tcagctaaag
    34261 gtgatcgaac agaaccaata tcaagaccct cattaatacg aagtacttct ttaataaccc
    34321 catacatatt gccgtgagca gaaacaagga caccgataat ctcattaata gtatattgaa
    34381 gggccttggc cttttctaaa tccttgtcag caataagttg gttcagcctt aaaaataatt
    34441 ctggcatagc cccgtaggta ccaccaatgc ctgcagcagc acccatcaag cgccctccta
    34501 gaaattgttc atctgggcca ttaaaaacaa tatgatcgtc cccaccaatc gcacagaagg
    34561 tttgaatatc ctgcactggc atagatgaat ttttaacgcc aatcacgcgc ttattagcca
    34621 acatagtctt ataaaggctt ggcgtcaagg caacccctgc cagttgagga atattataga
    34681 tgataaaatc tgtgtgagga gcagccgaag aaatagcatt ccaataatca gcaaccgcat
    34741 attctggtaa ccggaaataa ataggcggaa tagctgcaat ggcatccacg cccaaacgtt
    34801 ctgaatgagc tgccaattca atactatctt tagtgttgtt gcaggcaacg tgattaataa
    34861 tcgtcagctt gcccttagcg actgccatga cattttctaa caccaattga cggtcaaaaa
    34921 cgctctggta aatacactct ccagaagagc cattaatgta aagcccctga acccctttgt
    34981 caatatagta ctgggtcaag gcacgcacac gctcagggct gatattcccc tggtcatcgt
    35041 aacaagcgta aaaggcagga ataatgcctt ggtatttcgt aagatctgtc atagtaatgt
    35101 cctttattca atgtcattat taagtgggaa tcgcctaaga agagattcca tgataatcaa
    35161 gtagagataa gttagactac tgatgccgat gatagctagc aaagacacgc ctccaaataa
    35221 cgttaacaga gacaattgta ggctaaatat cgtgagacaa ataaaagcca agaaagcaaa
    35281 actccatgga aaaaagagtc ctgctaataa aaagcttcgc ttaaacagcg tagataggga
    35341 aaggtctctt ttgacggcct gaggataagc gtacaaaaag agtataacca ccaaaaataa
    35401 aacagcaacg caaagtactt taaacccttg aaaaactagg cctgattggt ttcgaattaa
    35461 gaaaagatct aagagacaaa tagtcattat cccaagctca ataaggccaa ggcgtagccc
    35521 acttttaaag taatatttaa aatgagagct ataagtagta acaagctgac taacttgacc
    35581 ttctcgccaa tccaacaaac tcgcatacaa agccatttta gcaaccccaa tcgttagaag
    35641 gggaaggcag gacaagacaa ataagagatt aaacacgagc aaatcaaaca gtgctgcgct
    35701 agccctcatc catttactat caagcttgaa gagagagtgt aaaagtccct gttttttcga
    35761 tgtcaaatca caacttcctt tctagaaata cgcttacaaa caaaagctaa ctctcctctt
    35821 aaacgtttaa cacatgactt gcttcccatc tgatttatct atgtatgcac ctaaattaat
    35881 aagcaaccta tagattaagc ttagcgacac gctttattta gtctaacctt tcacagcacc
    35941 catagtaatg ccttgggtaa atgatttttg gaagacaaga aagactgtta caataggcac
    36001 tgcagccatg gcagcccctg ccataatcaa gccataatta gtagccattt cagcttgcat
    36061 agtggcaacc ccaagtgaaa tcgttaaatt ttctcgagag gttaacatca ctaactgcat
    36121 aaaataatca ttccaagtat tgataaaggt aaaaatcgct agcgctgcaa agcctggttt
    36181 gacaataggg aaagccacat tgaaaaaggt acgaatttcg ccacatccat caatcttggc
    36241 ggattccaat aactcggttg gaatattttc tgagaactgt ttcataagga aaacaccaaa
    36301 tggccagccc acaagaggca aaataacagc cgccaaagtg tcatggattc ccataaaatt
    36361 aacaatccgc acaagaggca ctagaacaac ctgttttggc aaggccatag cagcaataaa
    36421 aatcgaaaac aaaagccgct gtccataaaa ccgctttttg gccaaagcat agccagccaa
    36481 cgaggaggtt ccacaaacca agaacatggt cgccaccgaa ataaagacac tattccacaa
    36541 ccattttaag gctggatttt gcactaccaa ggccttaaaa ttttcaatag taggcgcctt
    36601 tggccaccat tgcggtggaa tcataatggt atcggcttga gccttaaatg ctcctgtcat
    36661 aatccaataa aatggaaaaa taaacaaaat ggtcaaaaca catagcatca cagtggttaa
    36721 gatatctgat gcggttagtt tctttttcgt catcttaata tcctcctaat attccacatc
    36781 attacctaaa attttaaatt gagcaaagct gatgattgca atcatgactg ccaagaatac
    36841 acccattgtg ttggcatagc cgtattcaga gagcttaaag gccttttcat acaaatagta
    36901 catgagggta ctggttgaat aatttggccc tccagacgtc aaaagttgaa tcaaagcaaa
    36961 acattggaag gaattaatgg ttgttatgac agcaatatat aaggttgttg gcagcagact
    37021 tggccacttg atattccaaa acacctgaaa ttctgtggcg ccgtccacac gcgcagcttc
    37081 taccaaagag ttatcaatat ttcccatagc agcaatatat agaataatag gctgcccaac
    37141 agatgtggtt aataaaataa caataatagc caacaaagcc cagtgcttat ctcccagcca
    37201 actaatattt tgttcaatca catgagcata ttttaagaca taattcaaaa tccctgacat
    37261 tggatcatag atccatttcc aaaccaccgt caccgcaaca cttccggtaa caacaggcag
    37321 aaagaaaact gcccggtaaa acgaacgcgc cactacattt ttgtcgtagg ttttagccgc
    37381 cacaaagagg gaaaagaaaa ctacaacagg taccgaacca ataacaataa tcaaggtgtt
    37441 aataagagac ttcatgaaaa tgggatcttg aaacatccta gcataattag caaaaccaac
    37501 aaaggtaaac tctgtcatgg aataattgaa aaagctcgtc acaaagccca taatcatcgg
    37561 tatcaagaca aaaatcacaa agaaaaccaa gaccggagct aagaaagcgt atgagatgag
    37621 cgtctctctc atttttaatt tattgacgtt cacttgaaat acctcttttt tctgttcaac
    37681 ttttctaaaa agaaaagctt ctttccagtg accatttgac atgtgcattg aaaagaagcc
    37741 acttgttagg aacacttatt gtgttttttt gattgtcttg tttgcttttt cagtgaaggc
    37801 tttcaaagca tcttctggct tttcatcacc attagagacg gcttgaacca ttgggaacca
    37861 aagagttctc atttcagcaa acccatcaat cgtgttatag tatggtgagt agaattttgt
    37921 ccattcagca attttttcca ttcgtttgtc tttgtaaaga tccccataag aagttcttac
    37981 agggaaagca cctgtacgaa caacattttt aggtccccat tctttatcat cagcgataaa
    38041 ttggataaat gtcttagagg cagcgacttt ttgttcatct ttgttattaa agaccgcaaa
    38101 accatttacg aggtattcta gttctggttt gccatcatct gatgggaatg ggatttcaag
    38161 gtaatccact ttactagctt ctaacaattt agcttggatg cctggttgtg caggcgccca
    38221 aagaatggta aaggaagttt ggccattcgc aaagttttga atatcagctg aaccatcgta
    38281 ctgagagcca ttcatcatta aaccatcttt aatccaattg gaaatcttgg tcatggcttt
    38341 aatagagtta gcatcatctg tggtgtattt ggttactttg tcatcggtaa tatgactact
    38401 ataaaggtta gcaaagaagg cacgtggtcc ttggtcacca ccttgaccat tagcaaagaa
    38461 agaacctggg ttatatcctt tgtccttcaa tgctttcaag actttttcaa aatcatctgt
    38521 tgtccaaccc tctttcacaa gatcaaggac gccagcatct ttgagcattt ttttattcaa
    38581 cgccatgtag aatggagcag agctgatagg atacatgtaa gctgtatctc ctgcttttga
    38641 ggcttgaatc aatttgtcat tgttaacatc tttggtaaac tcttctgtga acaagtcatt
    38701 caagtcagcc aatttcccat ttttaccata ttgaatgata cgacctggag catcaaagag
    38761 gacatcagga gctgttccag cttcaatagc agttgtgatt ttctcaggac cagatgtaaa
    38821 atcaatggtt tccaatttta ccttgatttc tggattagct ttctcaaagg cagcaatcaa
    38881 tttcttctca taggttccca caccatcttc agctttttct tgcgtgaaga ccgggaaagc
    38941 ccaccatgtg atttctgtct tagcagtatc agatttactt gcaccagcct ctttctggct
    39001 ctttccgcca caagctgcta gtcctaaaac agatgcacca agcatcgcta atgaagctaa
    39061 cttcttcatg ttcatcgtaa aatctccttt ttttgatgtg tgtttctagt ttgacaacca
    39121 ctattaggat ttgagcgctt cgataaaacg ctctgcaatt tccttaggcc ttgtgatggc
    39181 ccctccaaca acaatacctg ccacacctaa gtcattaatt ttcttggctt cttctgggga
    39241 atgaatcttt ccttcggcaa tgacagcaat gcctgctttg caaagagctt ctatcaatgc
    39301 cacatcagga cctgcttcct gacggctata cggggtgtag cctgacaagg tggttccaac
    39361 aaaatcaatg ccagcctgat gggccactag tccttcatca aaggtactaa tatctgccat
    39421 caaaagctga ttggggtact tttccttaac ttgtcgaata aagctagcaa tatccaagcc
    39481 atcatgtcgg tcacgcttgg tacaatccat agcaatcact gcaatattta aggcagctaa
    39541 ttgatccact tcggtcatcg ttgccgtaat aaaaggctct tgcggcgggt aatctttttt
    39601 gatgataccg ataattggta ggtctgttat ggcttgaatt tctttaatgt ctctaaccga
    39661 attagctctg atgccgacag caccagcttc ttgagcagct tttgccatca gtggcataat
    39721 acccccagtc tctgaataga gaggctcacc tggcaaagcc tgacaagaaa cgataatccc
    39781 accctttaac tgctccatga gcttttcttt ggttggttta tcaggcatag tttctactcc
    39841 ttgtgtttct tagttttaat aaaaataacg ttcacaaagt tattataaca aaaaataaag
    39901 cgctttcaa
//
LOCUS       DAXWIQ010000013        39843 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000013 DAXWIQ010000000
VERSION     DAXWIQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39843
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.013"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            137..298
                     /gene="sagA"
                     /locus_tag="ACGEUI_000669"
     CDS             137..298
                     /gene="sagA"
                     /locus_tag="ACGEUI_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM family cytolysin streptolysin S"
                     /protein_id="HFF8165526.1"
                     /translation="MLKFTSNILATSVAETTQVAPGGCCCCCTTCCFSIATGSGNSQG
                     GSGSYTPGK"
     gene            520..1470
                     /gene="sagB"
                     /locus_tag="ACGEUI_000670"
     CDS             520..1470
                     /gene="sagB"
                     /locus_tag="ACGEUI_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin S biosynthesis dehydrogenase SagB"
                     /protein_id="HFF8165527.1"
                     /translation="MSFFTKEQQPKENCPPITVEKARQLFEFNTNHLSLSDYHHQTVL
                     KTSKQLVAQHLMPNATDNLSQHFLMNYKANNNYLGFQASIVDFFTDSAVANFSSSYVY
                     ESQEKIIRLPKPTKISTALSTCIIKRRSHRQFSDRQMPLQDLSNILYYACGVSSQASI
                     RDGASDKITLRNCASGGGLYPIHLVFYARNISKLIDGFYEYLPYQHALRCYRHSSEEN
                     VRDFAEYGAINAENCNIIIIYVYHYMKNTRKYGNQATAYAFIESGEIAQNIQLTATAL
                     TYGSIDIGGYNKEYLQELLDLDGLGEHVIHMTLVGTKESQ"
     gene            1467..2525
                     /locus_tag="ACGEUI_000671"
     CDS             1467..2525
                     /locus_tag="ACGEUI_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagC"
                     /protein_id="HFF8165528.1"
                     /translation="MKYQLNSNVRVVTFQDTFCFRKGIWDFNEAILDLTQEPQNLKIV
                     YQEIVSQLVKGVAIDTETYENTLEPETFANLMEVISGLYYNDMLMLEDDYNLEENVMK
                     ILMGNFRFMAQAGHMVSNDPVLFISDSTYVNESAELLAEQLHLQLQVASDDLKMLIQQ
                     TDVSSRLDALEHRRNMNCLSDALCDYQSIIICQERLNIMMLRHLNEVSVAMKKQLVIG
                     FVDGPFLHTCTLNPPHSADFDSLERRVLARLQDSTLYQHFANQVLPATQDVSQAYLPL
                     LNVLMNLVVSEAFIIAQTGSSKFEGRLLSIYLPTLEIQVQDILKMSNSQTQGALAKLK
                     YEDQQISTREIVKKLLDE"
     gene            2545..3903
                     /locus_tag="ACGEUI_000672"
     CDS             2545..3903
                     /locus_tag="ACGEUI_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HFF8165529.1"
                     /translation="MLYYYPSFNHIFDELKSLSGNRTGILNQSQVPVCNHPHDVYLKS
                     ITGQMPDYHKQFIGELSQVSYHIIGYGSYYEEALIKYLGESIERYATVIAGDLLSDRI
                     VYASYNELKLLHKVMPLEYLQVFTQEQIALSCDLQMMMCDKMVTENDVLGWVKCPMFF
                     EDAEMYVPAQMLCVGYKTNETVGERRIIPGFSTGTASHKTLEAAMCNSLIEYIQIDSM
                     MLSWYTKKPCPKIIVDDPDIEVILEEARLGKDSLYDIIPIDMTVGEDNPLYTFGIILK
                     NKYDEGPYLLFGVQAGLDPKHALLRGIMEASAISYSYYYNLLYQKASLANIECEEPLF
                     LDLDSNVFYYAHPKDQDHKWKAFEPLISGEVLLSDLEDHSGKDKKEDLKTLLAYAKKV
                     SPNAVFLDITPPEALEKGWYVTRVLMPELLEMCIPAFPFANHPRMRQFGGVTNAFVHP
                     MP"
     gene            3878..4549
                     /locus_tag="ACGEUI_000673"
     CDS             3878..4549
                     /locus_tag="ACGEUI_000673"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HFF8165530.1"
                     /translation="MPLSIQCLNLCFLLVTFCPSIPMQAIFGKEDSGYAFNLIGFLRA
                     TLIYDILTLVSIYVLSPQITLSLESIDSKTFFMGLVFCVLIVLIELVFLHGLRCWQKK
                     QWLPATFSFVGTTNDWSKIGYPLLLALFEETIYRFLWFNILAFQWHLPTIIVLIVTSF
                     CYALNHLLMGKSIFYAKLVTGIIYGSIYMLTSQLWLVVIMHVGGNLLVECLSHLQTKK
                     KKEVT"
     gene            4546..5229
                     /locus_tag="ACGEUI_000674"
     CDS             4546..5229
                     /locus_tag="ACGEUI_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagF family protein"
                     /protein_id="HFF8165531.1"
                     /translation="MMLLVLLSFLGLTTLWLTSYRRRCLARWHLQWLVALSYQDFLDV
                     LLSLFQFVVIILVLFFYSATINLGEVLTFLTQTSWHWQILCYLVLYLMAIIEMTLLVL
                     ILIFDVLLQKDSRLLFKKITWLPFRQDKPVLSILLLGTVILVDSLFYLGLLILLGEQS
                     LTSLATLILGYGIVKACRYSGWLNVLLAFCLFTIVGLWAVTATLLYGWLVGAMILTVT
                     YLMISCKEY"
     gene            5252..6175
                     /locus_tag="ACGEUI_000675"
     CDS             5252..6175
                     /locus_tag="ACGEUI_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8165532.1"
                     /translation="MSFVQLTNVVKSYKNGKKAVNDVSLSIEAGNIYGLLGPNGAGKS
                     TLINLILGLIPLSSGKITVLGQSQKTIRKISSQIGYVPQDIAVYPDLTAYENVELFGS
                     LYGLKGAQLKKQVLKSLEFVGLHSQAKQFPSQFSGGMKRRLNIACALVHSPKLIIFDE
                     PTVGIDPQSRNHILESIRLLNKEGATVIYTTHYMEEVEALCDYIFIMDHGQVIEEGPK
                     FELEKRYVANLANQIIVTLTDSRHLELADKPDWSLIEDGEKLMLKIDNSDMTSVVHQL
                     TQANITFSEIRHNHLNLEEIFLHLTGKKLRD"
     gene            6184..7311
                     /locus_tag="ACGEUI_000676"
     CDS             6184..7311
                     /locus_tag="ACGEUI_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagG family ABC transporter permease subunit"
                     /protein_id="HFF8165533.1"
                     /translation="MVLFHLIKKESLQIFRNRTALLMMVIFPILMIVILSFAFKSSFN
                     TATTVPKLTIRYQLEGEKTDYQKNFLAFLKVLNQKLHLETKPSNSLEKDRQRVSEGAL
                     TAVLEVKKNQTIKVITNNINQQNADLINMLVKNYVDNAKTYDSIAALYPQQLNHIRKR
                     SVDYVKVSSIQTSKGMTSADYYAISMFTMITFYSMMSAMNLVLSDRQQRITNRIHLTG
                     VSPSFLVFGKLIGAMLATTVQLSLLYIFTRFVLRVNWGTNEWMLIGITASLVYLSVAI
                     GIGLGISIKNEAFLTVASNTIIPIFAFLGGSYVPLTTLHSSIINQLSNISPIKWVNDS
                     LFYLIFGGQYNPIPVTLIVNISIGTIFIILALIGMRKQVTT"
     gene            7308..8426
                     /locus_tag="ACGEUI_000677"
     CDS             7308..8426
                     /locus_tag="ACGEUI_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8165534.1"
                     /translation="MICFIKTLFVKIKRKKTSYVTFFLMPILTTLLALSLSFSNNNQA
                     KIGILDKDNSQISKQFIAQLKQNKKYDIFTKIKKEHIDHYLQDKSLEAVLTIDKGFSD
                     KVLQGKSQKLNIRSIANSEITEWVKAQTNYLLENYNIIGDVALGNEDTFNRILQKNQQ
                     LNYDVKQVTLTDRSRSKAVSSTTTGFLLILMLGSTSVIYSGILADKSSQLYHRLMLSN
                     LSRFRYMLSYVCVGFVAFTIQIVIMLSLLKVFNISFFVPTSLLLIIFFLFSLLAIGFG
                     LLIGAITQNSQQSSQLANLIVMPTSMLAGCLWPLSITPSYMQAIGKLLPQNWVLSAIA
                     IFQSGGTLSQAWPYLLALMGTALALISFSSLLLKPTKL"
     gene            8997..11729
                     /locus_tag="ACGEUI_000678"
     CDS             8997..11729
                     /locus_tag="ACGEUI_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFF8165535.1"
                     /translation="MINKKCIIPVSLLTLAITLTSVEEVTSRQNLTYANEIVTQRPKR
                     ESVISDKSNFPVISPYLASVDFGERKTPLPTPDKGVKVTTEQSIAQVRKGPEERPYTV
                     TGKITSVINGWGGYGFYIQDSEGIGLYVYPQKDLGYSKGDIVQLTGTLTRFKGDLQLQ
                     QVTAHKKLELSFPTSVKEAVISELETTTPSTLVKLSHVTVGELSTDQYNNTSFLVRDD
                     SGKSIVVHIDHRTGVKGADVVTKISQGDLINLTAILSIVDGQLQLRPFSLEQLEVVKK
                     VTSSNSDASSRNIVKIGEIQGASHTSPLLKKAVTVEQVVVTYLDDSTHFYVQDLNGDG
                     DLATSDGIRVFAKNAKVQVGDVLTISGEVEEFFGRGYEERKQTDLTITQIVAKAVTKT
                     GTAQVPSPLVLGKDRIAPANIIDNDGLRVFDPEEDAIDYWESMEGMLVAVDDAKILGP
                     MKNKEIYVLPGSSTRPLNNSGGVLLPANSYNTDVIPVLFKKGKQIIKAGDSYKGRLAG
                     PVSYSYGNYKVFVDDSKNMPSLMDGHLKPEKTNLQKDLSKLSIASYNIENFSANPSST
                     KDEKVKRIAESFIHDLNAPDIIGLIEVQDNNGPTDDGTTDATQSAQRLIDAIKKLGGP
                     TYRYVDIAPENNVDGGQPGGNIRTGFLYQPERVSLSDKPKGGARDALTWVNGELNLSV
                     GRIDPTNAAWKDVRKSLAAEFIFQGRKVVVVANHLNSKRGDNALYGCVQPVTFKSEQR
                     RHVLANMLAQFAKEGAKHQANIVMLGDFNDFEFTKTIQLIEEGDMVNLVSRHDISDRY
                     SYFHQGNNQTLDNILVSRHLLDHYEFDMVHVNSPFMEAHGRASDHDPLLLQLSFSKEN
                     DKAESSKQSVKAKKTSKGKLLPKTGDSLVYVITLLGTASLLVPILLLTKGKKES"
     regulatory      11894..12009
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            12007..12507
                     /locus_tag="ACGEUI_000679"
     CDS             12007..12507
                     /locus_tag="ACGEUI_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFF8165536.1"
                     /translation="MTKLTVHDYVHIGLVAALYVVLTITPPLNAISYGMYQFRISEMM
                     NFLAFYHRKYIIAVTLGCMIANFYSFGLIDVFVGGGSTLIFVTLGVILFSKYQKDYLF
                     NGIFNKAFVYFSFFFATSMFTVAIELYFFGAPFLLTWFTTALGELVSLLIGSLIIDKL
                     SQRISF"
     gene            12701..14659
                     /gene="ligA"
                     /locus_tag="ACGEUI_000680"
     CDS             12701..14659
                     /gene="ligA"
                     /locus_tag="ACGEUI_000680"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFF8165537.1"
                     /translation="MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQ
                     SYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNA
                     TYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPYQLSEPLTIT
                     VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTSVVAKRQLATFLYQE
                     ASPTARNQQNEVLAELADLGFSVNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFL
                     AGFVHDVADIYQLTKEDFMQLDGIKEKSADKLLAAIEASKSNSAEKLLFGLGIRHIGS
                     KVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVDELKTAG
                     VNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVI
                     AGSDAGSKLEKAKSLGIRIEDEDWLRQL"
     gene            14673..15695
                     /locus_tag="ACGEUI_000681"
     CDS             14673..15695
                     /locus_tag="ACGEUI_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HFF8165538.1"
                     /translation="MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTT
                     AQKNSALNEARRAAKAGFDLLIAAGGDGTINEVVNGIAPLKKRPKMAIIPTGTTNDFA
                     RALKVPRGNPSQAAKLIGKNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKT
                     MFGYLAYLAKGVELLPRVSNVPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAK
                     LDDGMFTLILIKTANLFEIVHLLRLILDGGKHITDRRVEYIKTSKIVIEPQCGKRMMI
                     NLDGEYGGDAPITLENLKNHITFFADTDLISDDALVLDQDELEIEEIVKKFAHEVEDL
                     GQELEE"
     gene            16088..16285
                     /locus_tag="ACGEUI_000682"
     CDS             16088..16285
                     /locus_tag="ACGEUI_000682"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFF8165539.1"
                     /translation="MNPIFALALACFGVSLAEGFLMANLFKAASRQPEIIGQLRSLMI
                     LGVAFIEGTFFVTLVMAFILK"
     gene            16320..17036
                     /gene="atpB"
                     /locus_tag="ACGEUI_000683"
     CDS             16320..17036
                     /gene="atpB"
                     /locus_tag="ACGEUI_000683"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFF8165540.1"
                     /translation="MEEAKIPMLKLGPITFNLTLLAVCIVTIAIVFAFVFWASRQMKL
                     KPEGKQTALEYLISFVDGIGEEHLDHNLQKSYSLLLFTIFLFVAVANNLGLFTKLETV
                     NGYNLWTSPTANLAFDLALSLFITLMVHIEGVRRRGLVAHLKRLATPWPMTPMNLLEE
                     FTNFLSLAIRLFGNIFAGEVVTGLIVQLANYRVYWWPIAFLVNMAWTAFSVFISCIQA
                     FVFTKLTATYLGKKVNESEE"
     gene            17054..17548
                     /gene="atpF"
                     /locus_tag="ACGEUI_000684"
     CDS             17054..17548
                     /gene="atpF"
                     /locus_tag="ACGEUI_000684"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFF8165541.1"
                     /translation="MSITFGELVGNFILVTGSVIVLLLLIKKFAWGAIESILQTRSQQ
                     ISRDIDQAEQSRLSAQQLEAKSQANLDASRLQASKIISDAKEIGQLQGDKLVAEATDE
                     AKRLKEKALTDIEQSKSDAISAVKTEMSDLTVLLAEKIMGANLDKTAQSQLIDSYLDD
                     LGEA"
     gene            17548..18084
                     /locus_tag="ACGEUI_000685"
     CDS             17548..18084
                     /locus_tag="ACGEUI_000685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFF8165542.1"
                     /translation="MTKKEQALIEQYAKSLVEVASEHHSLDALQADVLAILETFVTTN
                     LDQSLSSQAVPHAEKIKLLTLLKGNNSVYMNNFLNLILQNEREAYLYQMLQAVLNEIA
                     IVSNQYDVTVTSSLPLTEEQKSRVRAVVAKKFAVTAGRLIEKVDPSLIGGFIISVNNK
                     VIDTSIRRQLQAFKMNLK"
     gene            18100..19608
                     /gene="atpA"
                     /locus_tag="ACGEUI_000686"
     CDS             18100..19608
                     /gene="atpA"
                     /locus_tag="ACGEUI_000686"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFF8165543.1"
                     /translation="MAINAQEISALIKKQIENFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFENGAYGMAQNLESNDVGIIILGDFSAIREGDVVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPVDGLGDIETTGFRPVETPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRRYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDDLGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     DDILAFEEALYDYFDVHYNDLFETIRTTKDLPEEAALDAAIKAFKEHSNFKS"
     gene            19624..20499
                     /locus_tag="ACGEUI_000687"
     CDS             19624..20499
                     /locus_tag="ACGEUI_000687"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFF8165544.1"
                     /translation="MAGSLSEIKAKIISTEKTSKITSAMRMVSSAKLVKSEQAARDFQ
                     IYASKIRQITTDLLKSELTIGSDNPMLVSRPVKKTGYIVITSDKGLVGGYNSKILKSV
                     MDMITEYHADGDYEIISIGSVGSDFFKARGMNVAFELRGLADQPSFEQVRQIISQSVD
                     MFVNEIFDELYVCYNHHVNSLTSQVRVQQMLPISDLVADEAAEEGVTGFELEPNRHDI
                     LDQLLPQFTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQAAI
                     TQEITEIVAGANALE"
     gene            20661..22067
                     /gene="atpD"
                     /locus_tag="ACGEUI_000688"
     CDS             20661..22067
                     /gene="atpD"
                     /locus_tag="ACGEUI_000688"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFF8165545.1"
                     /translation="MSSGKIAQVVGPVVDVMFASGDKLPEINNALIVYKDSDKKQKIV
                     LEVALELGDGMVRTIAMESTDGLTRGLEVLDTGRAISVPVGKETLGRVFNVLGETIDL
                     EEPFAEDVDRQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTQKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALSPEIVGEEHYAVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGLPGSYVPVADTVRGFKEILEGKYDELPEDAF
                     RSVGPIEDVIKKAEKMGF"
     gene            22080..22496
                     /locus_tag="ACGEUI_000689"
     CDS             22080..22496
                     /locus_tag="ACGEUI_000689"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFF8165546.1"
                     /translation="MTQMTVQVVTPDGIKYDHHAKFISVTTPDGEMGILPNHINLIAP
                     LQVHEMKIRRGGEDEKVDWIAINGGIIEIKDNVVTIVADSAERDRDIDVSRAERAKLR
                     AEREIAQAETTHNIDEVRRAKVALRRALNRINVSKK"
     gene            22783..23019
                     /locus_tag="ACGEUI_000690"
     CDS             22783..23019
                     /locus_tag="ACGEUI_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFF8165547.1"
                     /translation="MELLMKIIAFSCHLFFIGLSYQLLISVIDWTKFSHYHPENLGRL
                     RLFVFLLATALGYLVSHCMLELLQISQTLFFEFR"
     gene            23084..24355
                     /gene="murA"
                     /locus_tag="ACGEUI_000691"
     CDS             23084..24355
                     /gene="murA"
                     /locus_tag="ACGEUI_000691"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFF8165548.1"
                     /translation="MDKIIIEGGQTRLEGEVVIEGAKNAVLPLLAASILPSKGKTILR
                     NVPILSDVFTMNNVVRGLDIRVDFNEAANEITVDASGHILDEAPYEYVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPINLHLKGLEAMGATITQKGGDITAQADRLQGAMI
                     YMDFPSVGATQNLMMAATLADGVTTIENAAREPEIVDLAQFLNKMGARIRGAGTETLT
                     ITGVTHLRGVEHDVVQDRIEAGTFMVAAAMTSGNVLIRDAVWEHNRPLISKLMEMGVS
                     VTEEEYGIRVQANTPKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMVETVFEN
                     RFQHLEEMRRMGLQSEILRETAMIHGGRQLQGAPVMSTDLRASAALILTGIVAQGVTI
                     VNNLVHLDRGYYQFHEKLAKLGATISRSSEV"
     gene            24359..24547
                     /locus_tag="ACGEUI_000692"
     CDS             24359..24547
                     /locus_tag="ACGEUI_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFF8165549.1"
                     /translation="MTNGLKYVLEQMLLLFIIAALACLFLAIGLMIGYSFMGDGQSPW
                     HILSMDKWAELVNKFTGK"
     gene            24598..25122
                     /locus_tag="ACGEUI_000693"
                     /pseudo
     CDS             24598..25122
                     /locus_tag="ACGEUI_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009853913.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
     gene            25405..26448
                     /gene="pheS"
                     /locus_tag="ACGEUI_000694"
     CDS             25405..26448
                     /gene="pheS"
                     /locus_tag="ACGEUI_000694"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFF8165550.1"
                     /translation="MDLQAQLEELKTKTLETLQSLTGNHTKELQDLRVAVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDLLTKAFEEQAKIVEAAKIQAQLDAESIDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQI
                     EGLVVGKNISMGDLKGTLEMIIKKMFGDERSIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKKTGWIEILGAGMVHPSVLEMSGVDAKEYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDQRFSEQFN"
     gene            26658..29063
                     /gene="pheT"
                     /locus_tag="ACGEUI_000695"
     CDS             26658..29063
                     /gene="pheT"
                     /locus_tag="ACGEUI_000695"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFF8165551.1"
                     /translation="MLVSYKWLKELVDIDVTPAALAEKMSTTGIEVEGIEVPADGLSK
                     LVVGHVLSCEDVPETHLHLCQVDTGDETPRQIVCGAPNVKAGIKVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKYLDLDD
                     HIIELSITPNRADALSMRGVAHEVAAIYGKSVSFPQKNLQESDKATSEAIEVAIASDN
                     VLTYASRVVENVKVKPSPQWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDYDK
                     FEDHKIVARAARQGESLVTLDGEKRDLTTEDLVITVADKPVALAGVMGGQATEIDANS
                     QTVVLEAAVFDGKSIRKTSGRLNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEG
                     QVLSGHVQAGQLPTEPVEVSTSLDYVNVRLGTELTFADIQRIFDQLGFGLTGDETSFT
                     VAVPRRRWDVSIPADLVEEIARIYGYDKLPTTLPEAGGTAAELTPTQALRRKVRGLAE
                     GLGLTEIISYALTTPEKAVEFAVAPSHLTELMWPMSVERSALRQNMVSGMLDTVAYNV
                     ARKQSNLALYEIGKIFEQEANPKEDLPNEVNHFAFAICGLVAQKDFQTQAQAVDFYHA
                     KGNLDTLFANLNLKVQYVPTKDLANMHPGRTALILLDEQVIGFVGQVHPGTAKAYSIP
                     ETYVAELDMAALEAALPSDQTFAEITKFPAMTRDVALLLDREVSHQAIVTAIESAGVK
                     RLTSIKLFDVYEGATIQAGKKSMAYSLTFQNPNDNLTDEEVAKYMEKITKALTEQVGA
                     EVR"
     gene            29173..29550
                     /locus_tag="ACGEUI_000696"
     CDS             29173..29550
                     /locus_tag="ACGEUI_000696"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFF8165552.1"
                     /translation="MYQTKITGNRLYHTVSKGYGDSVTLFGKTDQGETPMSLLVIALS
                     SCVTMCVQGFYHRQFQQDTLAVSVEASYEEEVFELLVRIDDVLDDKKKADLLAYIGTY
                     CRVKQLLKPEVTVTITLEGLSHV"
     gene            29543..29929
                     /locus_tag="ACGEUI_000697"
     CDS             29543..29929
                     /locus_tag="ACGEUI_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFF8165553.1"
                     /translation="MSDASLKTYQKQWAYQKYWVMAHSQQHYNALRELFKGNQWSEEK
                     VLTFHCLIEEAQAIPPTVKSLRTAYQHVWGYFKKVASQEEKDHFKDLDAQLETKSEEM
                     LCFLQEMTAHYQPSYLLSCRLITKGP"
     gene            30002..31078
                     /locus_tag="ACGEUI_000698"
     CDS             30002..31078
                     /locus_tag="ACGEUI_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8165554.1"
                     /translation="MFLALNEMKQSKLRYGLIAGLLCLVAYLMFFLSGLAFGLMQENR
                     SAVDLWKADSVLLAKDADATLTLSQVSRAQENQITADKVAPLAQLNTVAWSVKNPKDA
                     DKVKVSLFGIDSNSFIRPNIVKGRLFKTNKEVVLDQSLAKEEAFAIGKDFYTSSSSQA
                     LTIVGYTQNARFSVAPVVYMNLEAFETLKYGEPLPKDKQVVNAFITKGSLTDYPKKDF
                     QKLDIKTFITKLPGYSAQLLTFGFMISFLVIISAIIIGIFMYILTIQKAPIFGIMKAQ
                     GISNKTITTAVLMQTFFLSFLGSGLGLLGTWLTSLLLPTVVPFQSNWFLYLAIFVSMI
                     CFALLGTLFSVFNIIRIDPLKAIG"
     gene            31088..31756
                     /locus_tag="ACGEUI_000699"
     CDS             31088..31756
                     /locus_tag="ACGEUI_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8165555.1"
                     /translation="MSVLTFKQVTKTFQDGHHEINALKATDFSIEAGEFVAIIGPSGS
                     GKSTFLTIAGGLQTPSSGQLIIDGTDYTHLSEKERSRLRFKSVGFILQASNLIPFLTV
                     QQQLELVDHLTGSKEKAKANQLFDDLGITGLKHQLPQELSGGERQRAAIARALYHDPA
                     LILADEPTASLDTEKAYEVVKLLAKESKEKNKAIIMVTHDDRMLKYCDKVYRMQDGEL
                     CQER"
     gene            complement(31858..32775)
                     /locus_tag="ACGEUI_000700"
     CDS             complement(31858..32775)
                     /locus_tag="ACGEUI_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFF8165556.1"
                     /translation="MKTDDLRESQQVEDRRGQSSGSFGGGGLGGGLLLQLLFSRGGWK
                     TKLVILLLLLVMGGGGLSGVLGGKPSSTNNNAYQSSQVTRTNGDKASQEQVSFVSKVF
                     ASTEDYWTKTFREKGLTYHKPTLVLYTGATQTACGRGQASSGPFYCPGDQKVYLDISF
                     YNELSTKYGAKGDFAMAYVIAHEVGHHIQNELGIMDNYASARQGKSKAKANQLNVKLE
                     LQSDYYAGAWANYVQGQGLLEKGDIEEAMAAAHAVGDDTLQEETYGRTVPDSFTHGTS
                     KQRQRWFDRGYQYGDFEHGDTFSIPYSKL"
     gene            32926..36141
                     /gene="rexB"
                     /locus_tag="ACGEUI_000701"
     CDS             32926..36141
                     /gene="rexB"
                     /locus_tag="ACGEUI_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HFF8165557.1"
                     /translation="MKLIYTEMSYSMTEILVNEARKAADQGYRVFYIAPNSLSFEKER
                     EVLTLLPERGTFSIIVTRFVQMSRYFTVESSPSKQHLDDTTLAMIFYRALMQLKPEDL
                     PSYGRLQNNSVFIEQLVELYKELKNAQLSVHDLTGLDHPQKQEDLIKIIELAETIMIQ
                     QDYNQDSPLQSFARAIKLGLLNNQLSKTVVVIDGFSRFSAEEDYLLSLLNNNCQEVII
                     GSYVSQKAYQKSFIKGNIYEASLHFLQDLAQKYHIKPVFATSNQVFKPAFSRLTQLFE
                     ATHDFSQVDWQLQKSDLDHFSLWQCHHQKEEIEHVAKSIRQKLYEGYRYKDILVLLGD
                     MDAYQLQIGPIFDKFEIPYYLGKAEPMAAHPLVQFIESLERSQRYNWRREDILNMLKS
                     GLFGCFDDSDIDRFEEYTQFADIKGFTKFSKPFTINSSRQYPLDFLNEMRQDIVLPLQ
                     ELFKSQKQLGASLVDKLILFLKKIRLAENMQGLAQSQLEVEKNEEVWKRFTDILTSFH
                     HIFGQEKLRLSDCLALIKTGMKSAQYRVVPATLDVVTIKSYDLVQPHSKPFVYAIGLT
                     QSHFPKQIHHSGLLSDQERARINEIRNYRHFDIASAENSKKNHQTALSLFNAATKELV
                     LSVSTVINETFDDLSPYLKELINFGLPLLDKGKNYLSYDNSDIGNYKALLSQIIAINR
                     QDLIEMSDQDKMFWTVVLRYLRKQLRKQQLELPTSDYRLSTKPLSKEVIEVCFPKGIP
                     LKLSATALTVFYNNQYNYFLKYVLNLNKTESIHPDSRIHGQYLHRVFERLMKDHTQEP
                     FDNKLKQAIYHTNQESFFQQVYQDNAEAEYSLAILEDIVRSTAPILQLNQNIQVIDQE
                     KNFQLDMGNEILVHGIIDRIDQLSDGSLGIVDYKSSANQFDIGTFYNGLSPQLMTYLA
                     ALKQIAPHDINQLFGAMYLHLQDPKLDLVTFKQIDNTLVESIYKALTYKGIFSEVEKE
                     HLSTGAYQTKNALYSNDELETLLNYNKYLYLKAAKHIKKGHFLINPYTSDGKTVQGDQ
                     LKAITRFEADLDMGQARRLVTLPAKEKKECFLTLMRKESHL"
     gene            36138..39770
                     /gene="addA"
                     /locus_tag="ACGEUI_000702"
     CDS             36138..39770
                     /gene="addA"
                     /locus_tag="ACGEUI_000702"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFF8165558.1"
                     /translation="MISFAPFLSPEAIKHLQENERCRDQSQKRTAQQIEAIYTSGQNI
                     LVSASAGSGKTFVMVERILDKILRGVSIDRLFISTFTVKAATELRERIENKLYSQIAQ
                     TTDFQMKVYLTEQLQSLCQADIGTMDAFAQKVVSRYGYSIGISSQFRIMQDKAEQDVL
                     KQEVFSKLFNEFMNQKEAPVFRALVKNFSGNCKDTSAFRELVYTCYSFSQSTENPKIW
                     LQENFLSAAKTYQRLEDIPDHDIELLLLAMQDTANQLRDVTDMEDYGQLTKAGSRSAK
                     YTKHLTIIEKLSDWVRDFKCLYGKAGLDRLIRDVTGLIPSGNDVTVSKVKYPVFKTLH
                     QKLKQFRHLETILMYQKDCFSLLEQLQDFVLAFSEAYLAVKIQESAFEFSDIAHFAIK
                     ILEENTDIRQSYQQHYHEVMVDEYQDNNHMQERLLTLLSNGHNRFMVGDIKQSIYRFR
                     QADPQIFNQKFRDYQKKPEQGKVILLKENFRSQSEVLNVSNAVFSHLMDESVGDVLYD
                     EQHQLIAGSHAQTVPYLDRRAQLLLYNSDKDDGNAPSDSEGISFSEVTIVAKEIIKLH
                     NDKGVPFEDITLLVSSRTRNDIISHTFNQYGIPIVTDGGQQNYLKSVEVMVMLDTLRT
                     INNPRNDYALVALLRSPMFAFDEDDLARIALQKDNELDKDCLYDKIQRAVIGRGAHPE
                     LIHDTLLGKLNVFLKTLKSWRRYAKLGSLYDLIWKIFNDRFYFDFVASQAKAEQAQAN
                     LYALALRANQFEKSGYKGLYRFIKMIDKVLETQNDLADVEVATPKQAVNLMTIHKSKG
                     LQFPYVFILNCDKRFSMTDIHKSFILNRQHGIGIKYLADIKGLLGETTLNSVKVSMET
                     LPYQLNKQELRLATLSEEMRLLYVAMTRAEKKVYFIGKASKSKSQEITDPKKLGKLLP
                     LALREQLLTFQDWLLAIADIFSTEDLYFDVRFIEDSDLTQESVGRLQTPQLLNPDDLK
                     DNRQSETIARALDMLEAVSQLNANYEAAIHLPTVRTPSQLKATYEPLLEPIGVDIIEK
                     SSRSLSDFTLPHFSKKAKVEASHIGSALHQLMQVLPLSKPINQQTLLDALRGIDSNEE
                     VKTALDLKKIESFFCDTSLGQFFQTYQKHLYREAPFAILKLDPISQEEYVLRGIIDAY
                     FLFDDHIVLVDYKTDKYKQPIELKKRYQQQLELYAEALTQTYKLPVTKRYLVLMGGGK
                     PEIVEV"
BASE COUNT        12442 a         6773 c         8155 g        12473 t
ORIGIN      
        1 ctagatagtt gttgtgttac aacagtacaa ttgagctagc cttgtccttg ttgtgttaac
       61 tttattttta aaataaggtt aaaaataaac gactcgcgtt cttatcagtt acttattaga
      121 taaggaggta aaccttatgt taaaatttac ttcaaatatt ttagctacta gtgtagctga
      181 aacaactcaa gttgctcctg gaggctgctg ttgctgctgt actacttgtt gcttctcgat
      241 tgctactgga agtggtaatt ctcaaggtgg tagcggaagt tatacgccag gtaaataatc
      301 tatttagcat ctctatgtga tagtgatatt aaggtaatga gtttgctagc aactaagtta
      361 tctttggtaa cttctgatta tagatgaatg gcatagaggt gttagaaaac atgagacaaa
      421 agtaatcttc ttgatgctaa cgtctcaggt aattagcagg tactagatag tacctgctaa
      481 ttactatatg tttagtaaaa tgagatagga aagatagtta tgtcattttt tacaaaggaa
      541 caacaaccta aagagaattg tccgccaata actgttgaaa aagcaaggca attgtttgaa
      601 tttaatacaa accacttgtc cttatcggat taccatcatc aaacggtgct aaaaacgtca
      661 aagcagctag ttgctcaaca tttaatgcct aatgcaaccg ataatcttag tcaacatttt
      721 ttgatgaact ataaagctaa taataattat ttaggcttcc aagctagtat tgtcgacttt
      781 tttacagatt ctgccgttgc taatttttca agtagttacg tttatgaaag tcaggaaaag
      841 ataattcgtt taccaaaacc taccaagata tcaactgctc tgtcgacatg tattataaaa
      901 cgaagaagtc atcgtcaatt ttcagataga caaatgcctc ttcaagattt atcaaacatt
      961 ctttattatg catgtggtgt tagttcacaa gcatcaatta gagatggagc atcagataag
     1021 attacactca gaaactgtgc ttcaggtgga ggtttatacc ctattcattt agttttttat
     1081 gctagaaaca tcagtaaatt aatagatggt ttctatgaat atctacccta tcagcatgca
     1141 ctaaggtgtt atcggcatag ctctgaggaa aacgttagag attttgcgga atacggtgct
     1201 attaatgctg aaaattgtaa tattattatt atttatgtct accattacat gaaaaataca
     1261 cgtaaatatg ggaatcaggc gactgcctat gcttttattg aatcaggaga aatagcccag
     1321 aatattcaat tgactgcaac tgccttaact tatggaagta ttgatattgg tggttataat
     1381 aaggaatatc tccaagaatt attagattta gatgggctag gagagcatgt gattcacatg
     1441 acactcgtag gaactaagga gtctcaatga aatatcaact taatagtaat gttcgtgtcg
     1501 tgacatttca agatacattt tgttttagaa aaggaatctg ggactttaat gaagccattc
     1561 tagatttgac acaagaacct caaaatttaa agatagttta tcaagaaata gtttcacagt
     1621 tggttaaggg agtagccatt gatactgaaa cttatgaaaa tactttagaa cctgagacgt
     1681 tcgctaattt aatggaagtc atttcaggct tgtattacaa tgatatgctg atgcttgagg
     1741 atgactacaa tcttgaagaa aatgtcatga aaattttaat gggtaatttc cgttttatgg
     1801 cgcaagcagg tcatatggta agcaatgatc ctgttttgtt tatcagtgat tcgacttatg
     1861 ttaatgaatc tgctgagttg ctagctgaac aactgcattt acaattgcaa gtggctagtg
     1921 atgacttaaa aatgttaatt cagcagacag atgttagttc gcgtttagat gctttggagc
     1981 atcgccgtaa catgaattgt ttatcagatg ccttgtgcga ttaccaaagt attattattt
     2041 gtcaagaacg cttgaatatt atgatgttgc gacacttgaa tgaagttagc gtagctatga
     2101 aaaaacaatt agtgattggt tttgtagatg gaccattctt gcacacttgt actttgaatc
     2161 ctccccatag tgctgacttt gatagcttag aaagaagagt tttggcacgt ttacaagact
     2221 caactctcta tcaacatttt gctaatcagg ttttaccagc cacacaagat gtgagccagg
     2281 cctatttacc attgttaaat gttttaatga atttagtcgt tagcgaagct ttcattattg
     2341 ctcaaacagg tagttctaaa tttgagggac gtttattaag catttactta ccgacgcttg
     2401 agattcaggt ccaagatatt ttgaaaatgt ctaattcaca gacacaagga gctttggcaa
     2461 aattaaagta tgaggatcag cagatatcaa cacgtgagat tgttaaaaaa ctccttgacg
     2521 agtaacctat aaggagaatc tattatgtta tactattatc cttcttttaa tcatattttt
     2581 gatgaattaa aaagtttaag tggcaatcga acaggtatct taaatcaatc tcaagttcca
     2641 gtttgcaacc atcctcacga tgtttactta aaaagtatta ctggccaaat gccagattat
     2701 cataaacaat ttataggtga gttgagtcaa gttagttatc acattattgg ttacggcagt
     2761 tactatgaag aagcacttat caagtattta ggagaaagta ttgaacgcta tgctactgta
     2821 attgccggag acttactatc agatcggatt gtctatgctt cttataatga gttaaaactg
     2881 ttacataaag tgatgccttt agaatacttg caagtgttta cccaagaaca gatcgcatta
     2941 tcttgtgact tgcaaatgat gatgtgcgat aaaatggtaa ctgagaacga tgtattgggc
     3001 tgggttaaat gtcccatgtt ctttgaggac gcagagatgt atgttccagc tcaaatgctt
     3061 tgtgttgggt ataaaacaaa tgagactgta ggagaaagac ggataatacc aggattttct
     3121 acgggaacag cttctcacaa aacgttagaa gcggctatgt gcaacagttt gattgaatac
     3181 atccagatcg attcaatgat gcttagctgg tatactaaga agccttgccc caagattatt
     3241 gttgatgatc ctgatattga agtgatttta gaagaagcta gactgggaaa agacagtcta
     3301 tacgacatta ttcctattga tatgactgta ggagaagata atcctcttta tacgtttgga
     3361 atcattctca aaaataaata tgatgaaggg ccttacctct tgtttggagt gcaggctggg
     3421 ttagatccta aacatgcttt gctaagaggg atcatggaag cttcagctat tagctatagt
     3481 tactactata atcttttata tcaaaaagct tcccttgcaa atattgaatg cgaagaacct
     3541 ttgtttttag atttagatag taatgtcttc tattatgcac atcccaagga ccaagaccat
     3601 aaatggaagg cctttgaacc tttgatttca ggagaagttc tgttaagtga tttggaagat
     3661 cattctggta aagataaaaa agaagatttg aagactttat tagcttatgc taagaaagtc
     3721 agtcctaatg ctgtcttttt agatattaca cctcctgaag ctctagaaaa aggctggtac
     3781 gtcacgcgag ttctgatgcc agaacttctt gaaatgtgta taccagcatt tccttttgct
     3841 aatcatccac gaatgagaca gtttggaggt gtaacaaatg cctttgtcca tccaatgcct
     3901 taatctttgt tttttgttgg taactttttg tccttcaatt cctatgcaag ctatttttgg
     3961 aaaggaagat agtggttatg catttaattt aattggtttt ctaagagcaa ctctgattta
     4021 tgacattttg actttggtaa gtatttatgt tttgtcacct caaataacct tgtctcttga
     4081 aagtattgat agtaagacgt tctttatggg acttgtattt tgcgtcttga tagtactgat
     4141 tgaactcgtt ttcttacatg gtttacgttg ttggcaaaaa aagcaatggc ttcctgcaac
     4201 tttctcgttt gtaggaacga caaatgattg gtctaagatt ggctatcctt tattactagc
     4261 tttatttgaa gagacgattt atcgtttttt gtggtttaat atattagctt ttcaatggca
     4321 tttaccaaca attattgttt taattgtaac tagtttttgc tatgctttaa atcacttatt
     4381 gatggggaaa tccatttttt atgctaagtt ggtaacaggc atcatttatg gtagtattta
     4441 catgctgact agtcaattgt ggttggttgt gataatgcat gtaggaggta acttgctagt
     4501 tgagtgtctt agccatcttc aaacaaaaaa gaagaaggag gtgacatgat gctattggtt
     4561 ttgctgtcgt ttttaggtct aactactctt tggttgacca gttatcgaag acgttgtttg
     4621 gctagatggc atcttcaatg gttagtggcc ttatcctatc aagatttttt agatgtctta
     4681 ctgagtctgt ttcaatttgt cgttattata cttgttttat tcttttactc agctactatt
     4741 aatctaggag aggttctgac ctttttgaca caaactagct ggcattggca gattctttgt
     4801 tatttagtgt tatatcttat ggccatcata gagatgacgc ttttagtttt aattcttatc
     4861 tttgatgttt tgctccagaa agacagtcga ttgctcttta aaaaaataac ttggctgcct
     4921 tttagacaag ataaaccagt cttaagtata cttttactag gcacagttat tctagtagac
     4981 agtctctttt atttgggctt gcttatatta ttaggagaac agtcgctaac aagtttagca
     5041 acgcttattt taggttatgg gatagtcaag gcttgtcgct attcaggttg gttaaatgta
     5101 ctattagctt tttgtctttt cactatagtt ggactatggg cagttacggc aactttatta
     5161 tatggttggc tagtaggtgc gatgatatta acggtgactt atttgatgat tagttgcaaa
     5221 gagtattagc aatattaaaa aggagtgata tatgagtttt gtacaattaa caaatgttgt
     5281 caagtcctac aaaaacggca agaaagctgt caatgatgtt tccttgtcta ttgaagcagg
     5341 taatatttat ggtttgttag gaccaaatgg tgctggtaag tctaccttga ttaatcttat
     5401 cttaggcttg atccctttga gttccggtaa aattactgtt ttagggcaat cccaaaagac
     5461 tattcgaaaa ataagttcgc agataggtta tgttcctcaa gacattgctg tttatccaga
     5521 cctaactgct tatgaaaatg tagaactatt tgggtcactt tatggcttaa agggagctca
     5581 gcttaaaaaa caagttctaa aaagtttaga atttgtgggg ctacactccc aagctaagca
     5641 gtttccaagt caattctcag gaggaatgaa gagacggtta aatatcgctt gtgcgctagt
     5701 tcattcacct aaattaatca tttttgacga accgactgta gggattgatc ctcaatcacg
     5761 taatcatatt ttagagtcga ttcgtttgct aaacaaagaa ggtgctacag ttatttatac
     5821 gacccattat atggaagaag tagaggctct ttgtgattat atttttatta tggatcatgg
     5881 tcaagttatt gaagaaggac ctaaatttga actggaaaaa cgttacgttg caaatctagc
     5941 aaaccagatt attgtaactc taacagattc acgtcatttg gaactggcag ataagcctga
     6001 ttggtctttg atagaagatg gagaaaaact catgttgaag attgataata gtgatatgac
     6061 atcagttgtt catcagctca cacaggccaa tattactttt agcgagatta gacataacca
     6121 tttgaattta gaagaaattt tcttacactt aacaggtaag aagttacgag attagaggtt
     6181 actatggttt tatttcattt aatcaaaaaa gaaagtttac agatttttag aaaccgaaca
     6241 gccttattga tgatggtgat ttttccaatt ttgatgatcg ttattttaag ttttgccttt
     6301 aaatcaagtt ttaatactgc gacaacagtc cctaaattga ccattcgtta ccaattagag
     6361 ggtgaaaaaa cggattacca gaaaaatttt cttgcttttt taaaagtttt aaaccaaaaa
     6421 cttcatttag agactaaacc tagtaattct cttgaaaaag atcgacaaag ggtcagtgaa
     6481 ggagccttaa cggctgtttt agaagtgaag aagaatcaga ccattaaggt tattactaat
     6541 aatattaatc agcaaaatgc agatttgatc aatatgctag taaaaaatta tgttgataat
     6601 gctaaaactt atgactcgat agcagctctt tatcctcaac aattaaatca tatcagaaag
     6661 cgaagtgtgg actatgttaa ggtcagttca atacagacaa gtaaaggaat gacatcagct
     6721 gattattatg ctatttccat gtttaccatg attacttttt atagtatgat gtctgcgatg
     6781 aaccttgttt tgtcagatcg tcaacaacgg attacaaatc gtattcactt aacaggagtt
     6841 tctccaagtt ttttggtctt tgggaaatta ataggtgcta tgttagcaac aactgttcaa
     6901 ttgagtcttt tatacatttt tacaaggttt gttttacgag ttaattgggg cactaatgag
     6961 tggatgctta ttggtataac agcctcctta gtctatctct ctgtagctat aggtatcggg
     7021 ctaggtataa gcattaaaaa tgaggctttt ttaacggttg catccaatac tattattcct
     7081 atattcgcct ttttaggagg cagttacgtt ccattgacaa cattacacag ctctattatt
     7141 aatcaattgt cgaatatatc tcctattaaa tgggttaatg atagtttgtt ctatcttatt
     7201 tttggtggtc aatataatcc gattcctgta actttgatcg ttaatattag tatcggaaca
     7261 atttttataa tattggcatt gataggtatg agaaagcagg tgacgacatg atttgtttta
     7321 taaaaactct atttgttaag ataaagcgca aaaaaacaag ttatgtgaca ttctttttaa
     7381 tgcctatttt aacgacactt ttggctttat ctttgagttt ttcaaataat aatcaagcta
     7441 aaattgggat attagataaa gataatagtc agatatctaa acaatttatt gctcaactaa
     7501 agcaaaacaa aaagtacgac atttttacca aaatcaaaaa agagcatatt gatcactacc
     7561 ttcaagataa atctctagaa gcggtattga ctattgacaa agggttctca gataaagtgt
     7621 tgcaaggaaa atctcaaaaa ctcaatatac gttcgattgc taatagcgaa attacagagt
     7681 gggtaaaggc acagaccaat tatctattgg aaaattataa tattataggg gatgtggctc
     7741 taggtaacga agatactttt aatagaattc tccaaaaaaa ccaacagtta aattatgatg
     7801 ttaagcaagt aactttgaca gaccgatcac gtagcaaagc agtatcttcc acaacgacag
     7861 gatttttgct tattctaatg ttaggtagta cgagcgtgat ttacagtggt attttggcag
     7921 ataaatctag tcagttatac catcgattaa tgttatcgaa cttatctcgt tttcgctata
     7981 tgttgagtta cgtctgtgta ggatttgttg cttttacgat tcaaattgtc attatgctga
     8041 gtttgttaaa agtatttaat attagttttt ttgttccgac atcacttctt ttgattattt
     8101 ttttcctttt tagtttatta gcgataggct ttggcctgct gattggagct atcacacaaa
     8161 attcccaaca aagtagccag ttagctaatc ttattgtaat gccgacttca atgttggctg
     8221 gctgtttatg gccattgtct attacgcctt cttatatgca agcaattggt aagttattac
     8281 cacaaaattg ggttttatca gccatagcta tttttcaaag tggagggacc ttatctcagg
     8341 cttggccata cttgttagct ctaatgggaa cagcactagc tttgattagt ttttcaagtt
     8401 tattattaaa acctaccaaa ctataattag tcttttgtta aaaaatactt tcctaattag
     8461 ctaactacca ggtagccctt ttattggctt atatgcaata aaagggttgc tttttgctta
     8521 tattttctct agtgcactta tcttttgtta aaagtgattg cgatgttact gttgttactg
     8581 tgttttttag agagcatatt atcaaaagcg acccctctat aaagttcgtt tttgttgata
     8641 aatgggttgt caaagtattt taaaaaacgt tttcaataaa cataaaaact aatgttatta
     8701 aaaaaagttt tgaaaacgta tttattttat gggatgtttt tgttacaata agcatgtatc
     8761 gaatgctata aagctgtctt gttcgctatc aggggtgctt gtgatgttat gtcagatcaa
     8821 acaagtcttt aattgggtga cttaacagat taaaagaaaa gatctaatcg tgttacttga
     8881 tagtataatt aatgcaaaca gaaagtgtat cccaattcat agtctcaggt ggaggttaat
     8941 attcaaaaat aaaatcttgt tgttgtctta aactaatcac attaaaagga gatatcatga
     9001 ttaacaagaa atgtataata cctgtttcat tgttgacact agctattacg cttactagtg
     9061 ttgaagaagt tacttcacgc caaaatttga cttatgccaa tgaaatcgta acacaaaggc
     9121 caaagagaga atctgttatt agtgataaat cgaattttcc cgtcatatca ccttacctag
     9181 caagtgtgga ttttggtgag agaaaaacac ctttgccaac acctgataaa ggagtaaaag
     9241 taactactga acagtctatt gctcaagtaa gaaaggggcc tgaagaaaga ccctatactg
     9301 ttactggcaa gattacgagt gtgatcaatg gctggggagg ctatggcttt tatattcaag
     9361 atagtgaagg tattggactt tatgtttatc ctcaaaaaga tttaggatac agtaagggag
     9421 atattgttca attaacaggt acacttactc gctttaaagg tgatttacaa ctccaacagg
     9481 tgactgcaca caaaaagtta gagttatctt ttccgacttc tgttaaagaa gcagtaatat
     9541 cagaattaga aacaacaaca ccctcaacat tagttaagtt atctcacgtg acagttggag
     9601 aattatcaac tgatcaatat aacaacacat ctttccttgt aagggatgat agtggtaaaa
     9661 gtatagttgt tcatatagat catcgtacag gggttaaagg ggctgatgtt gttactaaaa
     9721 taagtcaggg tgatttgatt aacctcacag ccatattgtc tattgttgat ggtcaattac
     9781 aattaagacc gttttctctt gaacaattgg aagtggttaa aaaggtcaca agctcaaata
     9841 gtgatgcttc atctcgtaat attgtgaaaa taggcgagat tcaaggagct agtcatacgt
     9901 cgccacttct caaaaaagcg gtcaccgtag aacaggttgt tgtcacttat ttagacgatt
     9961 ccactcattt ttatgttcaa gatcttaatg gtgatggtga tttagcgact tcagatggta
    10021 ttcgtgtttt tgctaaaaac gctaaggttc aagtcggcga tgttttgacc atttcaggtg
    10081 aagtggaaga attctttggt cgtggttatg aggaacgtaa gcagactgac cttaccatca
    10141 cccaaattgt ggctaaagca gtgaccaaaa cagggacagc tcaagttcca tcaccgcttg
    10201 ttttagggaa agatcgtatc gcgccagcca atattattga taatgatggc ttgcgtgtgt
    10261 ttgatccaga agaagacgct attgattatt gggaatcaat ggaaggcatg ttagtggcgg
    10321 ttgatgatgc taaaatcctt ggtccaatga aaaataaaga aatttatgtc ttacctggct
    10381 ctagtacaag accgttaaat aattcaggtg gagtattact tccagctaat tcttataaca
    10441 cagatgtgat tcctgttctt ttcaaaaaag gcaaacaaat tattaaagca ggagactctt
    10501 acaaaggaag attagctggg ccagtatctt atagctatgg taattacaag gtctttgttg
    10561 atgacagcaa aaacatgcca agtttaatgg atggtcatct aaaacctgaa aaaacaaact
    10621 tgcaaaaaga ccttagcaag ttaagcattg cttcttacaa tattgaaaac ttctcagcca
    10681 atccttcttc aactaaagat gagaaggtca aacggattgc cgaatccttt attcatgatc
    10741 tgaatgctcc agacattatt ggattaattg aagtccaaga taataatggg ccgactgatg
    10801 atgggacaac ggatgcgaca caaagcgcgc aacgcctcat tgatgctatt aaaaaactag
    10861 gtggcccaac ttatcgttat gttgatattg ctccagaaaa taatgttgac ggaggtcaac
    10921 caggtggtaa tattcgaaca ggattccttt atcaaccaga gcgcgtcagc ctttctgata
    10981 agccaaaagg cggtgctcgt gatgctctaa cttgggttaa tggagaatta aaccttagtg
    11041 ttggtcgaat tgatccaact aacgccgctt ggaaagatgt tcgtaaatca ctagcagcag
    11101 aatttatctt ccaaggtcgt aaagtcgttg ttgttgcaaa tcatttgaac tctaagcgtg
    11161 gggataatgc tctttatggt tgtgtgcaac cagtcacttt taaatctgag caaagacgtc
    11221 acgtcttggc taatatgcta gcacaatttg cgaaagaagg ggcaaaacac caagctaata
    11281 ttgtgatgct aggtgacttt aatgattttg aattcacaaa gacgattcaa ttaatcgaag
    11341 aaggtgacat ggttaacttg gtgagccgac atgatatttc agatcggtat tcttattttc
    11401 accaaggcaa taatcagacc cttgataata tattagtttc acgccattta cttgatcact
    11461 acgaatttga catggttcat gtgaattccc catttatgga agctcacgga cgcgcatcag
    11521 atcatgatcc attgttactt caattatcat tttccaaaga aaatgataag gcagagtctt
    11581 ctaaacaaag tgtaaaagct aaaaaaactt caaaaggaaa actgttgcca aaaacaggag
    11641 atagtcttgt ttatgtgata acgctactag gaacggctag tttattagtg cctattttat
    11701 tattgactaa aggcaaaaag gaatcatagc gatttgaaga attgcagttc ataaagagtc
    11761 gttaaaggtt tgatatactt acgttagatc ttaagctaaa atggatgact atataaaaac
    11821 taagattgat aacaaaagaa gataacaata agacagcttt acttgctaaa gacttttgtc
    11881 aagctaattg ttttatggta caatgtaacc gttgctttgc aacccttggt gcttagttcc
    11941 ctttcaccaa gcatattaga aacgtagata gcttttgaga gttatccaga cgttaaagga
    12001 gaaaaaatga caaaattgac tgtacacgac tatgttcaca ttgggctagt agctgccttg
    12061 tatgttgtct taactattac cccgccttta aatgcgattt cttatgggat gtatcaattt
    12121 agaatatctg aaatgatgaa tttcttagct ttttatcatc gcaagtatat tattgcggtg
    12181 actcttggat gtatgattgc taacttttat agtttcggac tgattgatgt ctttgtaggc
    12241 ggcggttcaa ctcttatttt tgtgacctta ggtgttattt tgtttagtaa gtatcaaaaa
    12301 gattatcttt ttaatggtat ttttaataaa gcttttgttt acttttcttt cttttttgca
    12361 acttccatgt ttactgtagc aatcgaactt tacttctttg gagcaccatt tttattaaca
    12421 tggtttacaa ctgcgttagg agaattagta tctctgctca taggatcact tattattgat
    12481 aagttgtctc aaagaatttc cttttagtaa tgcatcatac tcaaaagagt tgggtcaata
    12541 cccttctctt ttttatgttg tttgtaatgt taaaatcgtt ctagatagaa atatttaaca
    12601 ctttagctaa tagtaccata tggagtggcc aacgattact tttgatgttg ttatgatata
    12661 gaaatagctc atttgttatc ttttatgata aaataaaagt atgaaaaaac gcataaaaga
    12721 attaacagat cttcttaacc gttaccgtta tgactattat actaaagatg ccccatcagt
    12781 ttctgacagt gactatgata agctttatcg tgaactggtg actttagagc agtcatatcc
    12841 cgagtatgtc ttgcaagata gcccaacgca gcaggttgga gggactattt taaaggggtt
    12901 tgaaaaatat cgacatcagt atccgctttt tagtttgcag gatgcttttt cacgtgagga
    12961 gctggatgct tttgataagc gcgtcaaggc cgaatttccg aatgccacct atctagcaga
    13021 gttaaaaatt gatggtttat ctatttcctt gagttatgag aatggcttct tacaggttgg
    13081 agcaacacgg ggtgatggca atatcggtga aaatatcaca gaaaatatca aaaaaataaa
    13141 ggatattcct taccaattga gtgagccttt aaccatcacc gttcgaggag aggcttacat
    13201 gtctcgtcag tcctttaaag cgatcaatga agcacgccaa gaaaatgggg agactgaatt
    13261 tgctaatcct agaaatgctg ccgctgggac actaaggcag ctagatacgt cagtggttgc
    13321 taagcgtcaa ctagccactt ttttgtatca agaagctagt ccgacagcta gaaatcaaca
    13381 aaatgaggtc ttagcagaat tagcagatct aggtttttca gtaaatccgt attatcaact
    13441 cacatcgtct atggatgaga tttgggattt tatcaaaact attgaggcaa aaagagatca
    13501 attagcttat gacatagatg gcgttgttat taaagttaat agcctagcca tgcaagaaga
    13561 gctagggttt acggttaaag ctccacgctg ggccattgct tacaaatttc cagcagaaga
    13621 aaaagaggca gaaatccttt cggttgactg gacagtggga cgaacaggcg tggtcacacc
    13681 tacagcgaat ctaactcctg tgcaactagc ggggacaacc gtgagtcgtg caaccctaca
    13741 taatgtggat tatattgccg aaaaagatat ccgtattggc gatacagtga ttgtatataa
    13801 ggcaggggac attattcctg ctgtcttaaa tgtcgttatg agtaagcgga accagcaaga
    13861 agtcatgctt attcctaaac tatgcccatc ttgtggtagt gaattagttc attttgaaga
    13921 tgaggttgct ttgcgttgta ttaatccgtt gtgtcctagt ttgattcagc gaagtttgga
    13981 gcattttgca agtcgtgatg ccatgaacat tactgggctg ggaccagcta ttgtggaaaa
    14041 actattttta gcggggtttg ttcatgatgt tgctgatatt taccagttga ccaaagaaga
    14101 tttcatgcaa ttagatggca tcaaagaaaa gtcagctgat aaattgctcg ctgcgattga
    14161 ggcttctaaa tcaaattcag ctgaaaaact gttatttggt ctaggaattc gtcatattgg
    14221 ctctaaggtt agtcgcttaa ttttagaggt atacggagat atttctgctc ttttaaccgc
    14281 taaagaagag gaaatcgcaa ggatagatgg tcttggctca acgattgctc aatcacttac
    14341 tcaatacttt gaacaaaaaa cggctgcgat tttagtagac gagttaaaga cagcaggtgt
    14401 caacatgcat tactcaggtc aaaaagttaa tagtgacgca gcgctttttg gtctaactgt
    14461 tgttttaact ggcaaattaa atcagctgaa ccgaaatgag gccaaagata aattagaggc
    14521 attaggtgcc aaagttactg gaagtgtatc taagaaaaca gatttagtta ttgcaggtag
    14581 tgacgctggt tctaagctag aaaaagcaaa gagcttagga attcggattg aagatgaaga
    14641 ttggctacgc cagctttaag tgaggtaatg ttatgaaaaa acaattgaga gcacgtctca
    14701 tttataatcc aacgtcagga caagaactta tgagaaaaag tgttcctgaa gttttagaca
    14761 tattagaagg gttcgggtat gaaacatctg cctttcaaac aacagcccaa aagaactctg
    14821 ctctaaatga agcaagaaga gcggcaaagg caggttttga cttattgatt gctgctggag
    14881 gtgatggcac tattaacgaa gtggtcaatg gaattgctcc cttgaaaaag cgccctaaaa
    14941 tggccattat tccaacaggt accactaatg attttgctag agcattaaaa gtaccaagag
    15001 gaaatccgag ccaagcagca aaacttatcg gaaaaaatca gactatccag atggatattg
    15061 gtagagctaa gaaagatact tatttcatca atattgctgc agcaggaagt ttgacggaat
    15121 taacttacag tgtaccaagt cagttaaaaa cgatgtttgg atacttggct tatcttgcta
    15181 aaggagtaga actgctccca agagttagca atgtgcctgt taaaattacg catgacaaag
    15241 gcgtctttga aggtcaagtt tctatgattt ttgcagctat taccaattct gttgggggat
    15301 ttgaaatgat tgctcctgat gcaaaactag atgacggtat gtttacgctc attttaataa
    15361 agacagcaaa tctttttgaa attgtacatc ttttgcgttt gattttggat ggtggtaagc
    15421 atattacaga tcgccgtgtc gaatacatta agaccagtaa aatagtcatt gaaccccaat
    15481 gtggtaagcg aatgatgatt aatttagatg gcgagtacgg aggagatgct ccaattacat
    15541 tagagaacct aaaaaatcat atcacttttt ttgcagatac tgatttgatt tctgatgacg
    15601 ctttagtctt agatcaggac gaattggaaa ttgaggaaat cgtaaagaaa tttgctcatg
    15661 aagtagaaga cttaggacaa gaattagaag aataatacaa tagttattag ctactgacag
    15721 tttagtcaaa tgcactacta tctagaatct aagattttta aatcaattga ggtagtgttt
    15781 tttgatgcgc tcaaagagtc aatttattca tatgaggggc ctatttgagg tctctctttt
    15841 ttgatcatat gcaagaaaat gccatgtttt aggggtgtta ttataccctt tttggaatct
    15901 tgttgccttt ttgttataat aagagtgtaa ttctattttt aaaaagatct taacaaagac
    15961 agactcgttt atcaaaaagg ttagtattag tctaagaaag cgtacccaga ctaaagaaat
    16021 ctgttgacat tcttaatttt aaggaataga atagcaaagt aaaaaaaatt ttaaggagta
    16081 ttctactatg aatcctattt tcgcattggc tttggcctgt tttggtgttt ctcttgctga
    16141 aggtttcttg atggcaaatc ttttcaaagc agcttctcgc caaccagaaa tcattggtca
    16201 attacgttca ttaatgatct tgggtgttgc ctttatcgaa ggtactttct tcgttaccct
    16261 tgttatggcc ttcattctta aataacagcc tattttataa tagaaaagga gggtgttaga
    16321 tggaagaagc taaaatacct atgctgaagc ttggtccaat aacctttaat ttgaccctac
    16381 ttgctgtttg tattgtcaca attgcgattg tctttgcctt tgttttttgg gcaagtcgcc
    16441 aaatgaaatt gaaaccagaa gggaagcaaa ctgctttaga atatttaatc agttttgtgg
    16501 atggtattgg agaagagcac ttagatcata atctacaaaa atcttattcg ctgttactct
    16561 ttaccatttt tctctttgta gctgtcgcta ataatttagg gttatttact aagttagaga
    16621 cagttaatgg ctataaccta tggacctcgc caacagccaa tctggctttt gaccttgctc
    16681 tatctctttt tattacctta atggtacaca ttgaaggggt tagacggcgt ggcttggttg
    16741 cccatttgaa acgtttggct acaccgtggc caatgactcc gatgaattta ttagaagagt
    16801 tcacaaattt cttatcactt gccattaggt tattcggtaa tatctttgcc ggggaagttg
    16861 ttacaggttt gattgttcaa ctggccaatt atcgagttta ttggtggccg attgctttcc
    16921 tagtcaatat ggcgtggaca gccttttcag tctttatttc ctgcatacag gctttcgttt
    16981 tcacaaaact gacagcgacc tatctaggaa agaaagtcaa tgaatcagaa gaataaggaa
    17041 ggagaacaaa cgaatgtcaa tcacatttgg tgaactcgtt gggaatttta ttctagtaac
    17101 aggttctgta atcgtcttgt tactcttgat caaaaaattc gcttggggtg cgattgaatc
    17161 gattttacag acacgctcac aacaaatctc tcgagatatt gatcaggctg agcaatcacg
    17221 tctaagtgct caacagttag aggcaaaaag tcaagctaac ctagatgcta gtcgtttaca
    17281 agcaagtaaa atcattagtg atgccaaaga aattggtcaa ttacaaggtg ataaattggt
    17341 ggcagaagct actgatgaag caaaacgctt gaaagaaaaa gcgttgacag atattgaaca
    17401 aagcaaatca gacgctattt cagcagtcaa aacagaaatg tctgatttaa cggttctttt
    17461 agcggaaaaa attatgggag ccaatcttga taagacggcg caaagccagc ttattgacag
    17521 ttatcttgat gacttaggag aagcttaatg accaaaaaag aacaggctct tatcgagcaa
    17581 tatgcaaaaa gccttgttga ggtggcgagt gaacatcatt ctcttgatgc cttacaagct
    17641 gatgtgcttg cgattttaga gacgtttgta acaactaatc ttgatcaaag tctatcaagt
    17701 caagctgttc cgcatgctga aaaaataaaa ttgctaacct tattaaaggg aaataattct
    17761 gtttatatga ataatttctt gaatctcatt ttacaaaacg aacgtgaagc ttatttgtat
    17821 caaatgcttc aggctgtttt aaatgaaatt gcaatagttt caaatcagta tgatgtgact
    17881 gttacctcat cactaccttt gactgaggag caaaaaagcc gtgttcgagc agttgttgca
    17941 aagaaatttg ctgtaacagc gggtcgttta attgaaaaag tagatccttc gcttattgga
    18001 ggatttatta tcagcgtcaa taataaagta attgatacca gtattcgtcg tcaattgcaa
    18061 gcatttaaaa tgaatttaaa atagaaagtg gtgtgacttt tggcaattaa tgcacaagaa
    18121 attagcgctt taattaaaaa gcaaattgag aacttccagc caaatttcga tgtcacagaa
    18181 actgggatcg ttacctatat tggtgacggt attgcacgtg cccgtggatt agataatgcc
    18241 atgagtggtg aacttcttga atttgaaaat ggtgcctacg ggatggctca aaaccttgag
    18301 tcaaatgatg tgggtattat tattcttggt gatttttcag caatccgtga aggagatgtg
    18361 gttaagcgca ccggtaaaat catggaagta ccagttggtg aagctctcat tggacgtgtg
    18421 gtaaacccac ttggacaacc agttgatggc ttgggagata ttgaaacaac tggctttaga
    18481 ccagtagaaa cgccagctcc tggtgttatg cagcgtaaat ccgtttctga acccttacaa
    18541 actggattaa aagcaattga tgctttggtt ccaattggtc gtgggcaacg tgagttgatt
    18601 atcggagacc gtcaaacagg aaaaacatcc gtagctattg atgcgatttt gaaccaaaaa
    18661 ggtcaagaca tgatttgtat ctatgtggct attggtcaaa aagaatcaac cgttcgtaca
    18721 caagttgaaa cccttcgccg ttacggtgcc ttggattata ctattgttgt gacagcgtca
    18781 gcctcacaac catcaccatt gctctttatc gctccttatg cgggtgttgc catggctgaa
    18841 gaatttatgt accaaggcaa gcatgtcttg attgtctatg atgatttatc aaaacaagcg
    18901 gtggcttatc gtgaattgtc tctcttgctt cgtcgtccac caggtcgtga ggcttaccca
    18961 ggggatgttt tctacttgca tagccgtctt ttggaacgct ctgcaaaagt ttctgatgac
    19021 ttaggtggag gttctatcac agctctacca tttatcgaaa cacaggccgg agatatctct
    19081 gcatatattg cgactaacgt tatttcgatt acagacggac aaatcttctt gcaagagaac
    19141 ctctttaatt caggaattcg tccagcgatt gatgcaggat catctgtctc acgtgtaggt
    19201 ggttccgctc aaattaaagc gatgaaaaag gttgctggta ctcttcgttt ggatttggct
    19261 tcctatcgtg aattagaagc ctttacccaa tttggttcgg accttgatgc tgcaacacaa
    19321 gcaaaactta atcgtggtcg ccgtacggtt gaaatcttga agcaaccatt gcataaaccg
    19381 ttacctgttg aaaagcaagt cgttattctt tatgccctta ctcatggttt cttagatgat
    19441 gttcctgtag atgatatttt ggcttttgaa gaagctcttt acgactactt tgacgttcat
    19501 tacaatgatt tatttgaaac tatccgtacc acaaaagacc ttcctgaaga agctgccctt
    19561 gatgccgcta ttaaagcgtt taaagaacac tcaaacttta agtcatagag ggaggatact
    19621 agtatggcag gctctctaag tgaaattaaa gctaaaatta tttcaactga gaaaacaagt
    19681 aaaatcacta gtgccatgcg tatggtttct tctgcaaaac ttgttaaatc tgagcaagca
    19741 gctcgtgatt ttcaaattta cgcctcaaaa attcgccaga ttactacaga tttattgaaa
    19801 tcagaattaa ccataggatc tgataatccg atgttggtgt ctcgtcctgt caaaaaaaca
    19861 ggctatattg tcatcacgtc tgataaagga cttgttggag gttacaattc aaaaatcttg
    19921 aaatctgtta tggatatgat tacagagtac catgctgatg gtgactatga aatcatttct
    19981 attggtagtg ttggttcaga cttcttcaaa gcaagaggga tgaatgtggc ttttgaactt
    20041 cgtggacttg cggatcaacc aagttttgaa caagtgagac aaatcatttc acaatccgtt
    20101 gacatgtttg tcaatgagat ttttgatgag ttatatgtct gttacaatca ccatgtgaat
    20161 agcttaacca gccaagtccg tgttcaacag atgttaccaa tttctgactt ggttgctgat
    20221 gaagcagcag aagaaggtgt tactgggttt gaattggaac ctaatcgtca tgatatttta
    20281 gatcaattgt tacctcaatt cacagaaagt ttaatttatg gggctatcat tgatgctaaa
    20341 acggctgaac atgcagcagg tatgactgcc atgcagacag caactgataa cgctaagaat
    20401 gtcattaacg atttgaccat ccaatataac cgagctcgtc aagcagcaat tacacaagaa
    20461 attactgaaa ttgttgcagg agcaaatgca ctagaataaa aaataacgct gactagcgat
    20521 taggttattg tcagcattca attttatcaa tcgagataaa atgacttatg ggacttttct
    20581 attgaaagac tgctcttttc agtataaaag aagttagcaa gactaactaa gccctgacta
    20641 tcgaatataa ggagaaatga atgagctcag gcaaaattgc tcaggttgtt ggtccagtag
    20701 tagacgttat gtttgctagc ggggacaaac ttccagagat taataatgca ttgattgttt
    20761 ataaagatag tgataaaaag caaaaaatcg tccttgaagt tgctctggaa cttggtgacg
    20821 gtatggtgcg aacaatcgct atggaatcaa ctgatgggct tacacgtggg ttagaagttc
    20881 ttgacactgg tcgtgcgatt agcgtaccag taggtaaaga aactttggga cgcgtcttta
    20941 atgtacttgg agaaaccatt gacttggaag aaccatttgc agaagacgtt gaccgtcagc
    21001 cgatccataa aaaagcacca tcgtttgatg aattatcaac atcatcagaa attcttgaaa
    21061 ctggtatcaa ggtaattgac cttcttgccc cttaccttaa aggtggtaaa gttggacttt
    21121 tcggtggtgc cggagttggt aagactgtcc ttatccaaga attaatccac aatatcgccc
    21181 aagaacacgg aggtatttca gtatttaccg gtgttggtga gcgaacacgt gaaggaaatg
    21241 acctttactg ggaaatgaaa gaatcaggcg ttattgagaa aactgccatg gtttttggtc
    21301 aaatgaatga gccgcctggg gcacgtatgc gtgtagccct tactggttta accattgctg
    21361 agtatttccg tgatgtagaa ggccaagatg ttttgctctt tattgataat atcttccgtt
    21421 tcactcaggc aggttcagaa gtatcagccc tcttaggccg tatgccttct gctgttggtt
    21481 accaaccgac ccttgctact gaaatgggac aattgcaaga acgtattacg tcaactcaaa
    21541 aaggatctgt tacttctatc caagcgattt atgtgccagc cgatgactat actgacccag
    21601 cgccagcaac tgcatttgct cacttggatt caacaactaa ccttgagcgt aaattgactc
    21661 aaatgggtat ttaccctgcg gttgacccat tggcttcaag ttcacgggcc ttatcacctg
    21721 aaattgttgg tgaagagcac tatgctgtgg caacagaggt tcaacgtgta cttcagcgct
    21781 accgtgaatt acaagacatc atcgctattt tggggatgga tgaattatca gatgaagaaa
    21841 aaacattggt tggacgtgcg cgtcgtatcc agttcttctt atcacaaaac tttaatgttg
    21901 ctgaacaatt tacaggtctt cctggttctt acgtgccagt tgctgataca gttcgcggct
    21961 ttaaagaaat tcttgaagga aaatacgatg aacttccaga agatgccttc cgttcagttg
    22021 gtccaatcga ggatgtgatt aaaaaagccg aaaaaatggg cttttaatga ggtgatgcca
    22081 tgacacaaat gactgttcaa gtggtaacgc cagatggaat caaatatgat caccatgcta
    22141 aatttatttc ggtaacaaca ccagatgggg aaatggggat tttacctaat cacatcaatt
    22201 tgattgcacc tttgcaagtt catgaaatga aaattcgccg aggtggtgaa gatgaaaaag
    22261 tggactggat tgcaatcaat ggtggtatta ttgaaatcaa ggataatgtg gttaccattg
    22321 tcgctgattc tgcagaacgt gaccgtgata tcgatgtcag ccgagcagag cgagcaaaac
    22381 taagagcaga acgtgaaatt gcacaggctg aaacaaccca taatattgat gaggttcgtc
    22441 gtgccaaggt tgccttgcgc cgcgctttga atcgtatcaa cgttagtaaa aaataagtgg
    22501 tgttgataaa agacaaggat aagtaagaaa ggtttttaac ctattatttt gtttactgat
    22561 tctttttaac atcagtttct gagagtttga ccatactacc ttctctaaac acgttattat
    22621 tgttgagagg agttatatgg gggaactctt ttttgtatcc acgttgttag tagaaactca
    22681 gtaatgacgc ttaatatatt tttaggctat caacttagtc agagaagatg tcaacttaat
    22741 tgataagttg gacggatcaa gatggtatgt tataataatc ctatggaatt attaatgaag
    22801 atcattgcct tttcgtgtca tttatttttt attggcctta gttaccaatt attgattagt
    22861 gtgattgact ggacaaaatt tagtcattat catccagaaa atcttggccg gctacgactt
    22921 tttgtctttt tattagctac tgccctaggg tatttggtga gccattgtat gctggaattg
    22981 cttcaaatta gccagacgct cttttttgag ttccgctaat actggaataa aagaagtatg
    23041 gtataatttt actacgacta agagatagat aagggagaaa attgtggata aaataatcat
    23101 tgaaggtggt caaacacgct tagaaggaga agttgttatt gagggtgcta aaaacgcagt
    23161 tcttccttta ctggcagcat ctattttacc ttcaaaagga aaaacaattc ttagaaacgt
    23221 tcctattcta tctgatgttt ttaccatgaa taacgtggta cgtggtttag atattcgtgt
    23281 tgattttaat gaagcagcta acgaaatcac agttgatgcc tcaggacaca ttttagatga
    23341 agccccttac gagtacgtga gccaaatgcg tgcctctatt gttgttttag gaccaatttt
    23401 ggcacgtaat ggccacgcaa aagtatcaat gcctggaggc tgtacgattg gcagtaggcc
    23461 tattaatttg cacttaaaag gattagaggc tatgggggca acgattactc aaaaaggtgg
    23521 agatattacc gctcaagcag atcgactgca aggtgccatg atttatatgg acttcccatc
    23581 tgtaggagct acccaaaact tgatgatggc tgccacttta gctgatggtg tgaccactat
    23641 tgaaaatgct gctcgtgagc ctgaaattgt tgatttagct caatttttga ataaaatggg
    23701 agctagaatt cgtggtgctg gaacagagac gttaacgata acaggtgtca cgcatttacg
    23761 aggcgtagaa catgatgttg ttcaagatcg tatagaagcc ggtacattta tggttgcagc
    23821 ggcgatgact tcaggcaatg ttttgattag agatgctgtt tgggaacata atcgtccatt
    23881 gatttcaaaa ttaatggaaa tgggtgtttc tgtaacagaa gaagaatacg gtattcgcgt
    23941 tcaagcaaac acgccaaagc tcaaacctgt aacggttaaa accttgcctc atccaggttt
    24001 tccaacagac atgcaagctc aatttacagc tttaatggca gtggtcaatg gagagtctac
    24061 tatggttgaa actgtctttg aaaatcgatt tcaacatctg gaagaaatgc gacgcatggg
    24121 cttacaatct gaaatcctta gggaaactgc catgattcat ggtggaagac aactgcaagg
    24181 tgctcctgtg atgtcaacag atttacgtgc cagtgctgcc cttatcttga cagggattgt
    24241 tgctcaagga gttacaatag ttaataattt ggttcattta gatcgtggct actatcaatt
    24301 tcatgaaaaa ttagcaaagc ttggagctac tattagtcgt agcagtgagg tctagcttat
    24361 gacaaatggt ttaaaatatg tcttagaaca aatgttatta cttttcataa ttgctgcctt
    24421 ggcgtgtctt tttttagcca ttggacttat gattggttat agttttatgg gagatggcca
    24481 gagtccatgg catattttat ctatggataa gtgggcagag ttagtgaata aatttactgg
    24541 aaagtaggcc ttgcctactt tttatagcat ctgagaggaa gtatgttaac aaggaaaaaa
    24601 cgaaaaaggg tgcaagagca aaaggttcct aaaagagcta atgccttact tactaagtcg
    24661 acacgacagt atcgaaatcg ctatcaaacg gataatgctt atcgtaactt taagccagca
    24721 ggttggcacc gattgcacca cttaaaagga agttatgatc acgcggttga tagagggcat
    24781 ttgctaggtt atgctttagt tggtggactg aaaggttttg atgcttctac tggtaatcct
    24841 gataatattg ccactcaact aagttgggct aatcaagcaa ataaacccta cttgacaggt
    24901 cagaattact atgaaggttt agtacgtcgt gctttagata aggggcatcg cgtccgctac
    24961 cgtgtcaccc tactttatga tggtgataat ctactagcca gcggtagcca tttggaggct
    25021 aaatcctctg atgacagctt gacttttaac gtgtttgttc caaatgtcca agcaggattg
    25081 accgttgact atcggacggg acaaatagct atcaatctct aagcgaaaag tcactgattt
    25141 aagattgtca ctaagcactt tttttgatag aatagaacta attgaataag tgtctatgag
    25201 actagtacta atagagaaat taacagggag tgagggccgt gactggaagc cttcttaagg
    25261 gaactattag gaattcactc agctaaagac aattaattaa ggtctgataa cgtcagataa
    25321 aaacggatgg taccgcgtgt caacgctccg cttatcaagt ggaggagtgg catgcttttt
    25381 tcatcgccaa aagaagggaa aactatggat ttacaagcac aattagagga attaaagaca
    25441 aaaacattgg agaccttaca gtctttgacg ggtaatcata ccaaagaact gcaagatttg
    25501 cgcgtggctg ttcttgggaa aaagggatct ttgacagaat tactaaaggg gctaaaagac
    25561 ttgtctaatg acttgcgtcc tgtggttggt aaacaggtga acgaagttcg tgacttattg
    25621 acaaaagcct ttgaagaaca ggctaaaatt gtagaagctg ctaaaattca ggcccaactt
    25681 gatgctgaaa gcattgatgt gactttaccg ggtcgtcaaa tgactctggg gcatcgtcat
    25741 gtgttaacac aaaccagtga agaaatcgaa gatattttct taggaatggg ttttcagatt
    25801 gttgatggct ttgaggtgga aaaagattat tacaactttg agcgcatgaa tttgccaaag
    25861 gatcaccctg cccgtgacat gcaagatacg ttttatatca cagaagaaat cttgttacga
    25921 actcacacca gtcctgtgca ggcccgtact cttgatcaac atgatttttc aaaagggcct
    25981 ttaaaaatgg tttctccagg tcgtgttttc cgtcgtgata ctgatgacgc tacccactca
    26041 catcaattcc atcaaattga aggtttggtt gtgggcaaaa atatttccat gggagacctc
    26101 aaagggaccc ttgaaatgat catcaagaag atgttcggtg acgaacgtag cattcgtttg
    26161 cgcccgtcat atttcccgtt cacagaacca tctgttgaag tggatgtttc ttgctttaaa
    26221 tgtggtggga aaggctgtaa tgtctgcaag aaaacaggat ggattgaaat tcttggtgct
    26281 ggtatggttc acccaagtgt cttggaaatg tcaggtgttg atgcaaaaga gtattcaggc
    26341 tttgcctttg gtttaggtca agagcggatt gccatgttgc gttatggcat taatgacatt
    26401 cgtggtttct atcaaggcga ccaacgtttc tctgaacaat ttaactaaaa cgagtcgttt
    26461 gaagacctct gtcagctatt ttatctaaga ttgagtgaaa gttgataggt aatgataata
    26521 ggttaattgt ctaaggggaa agtccctctc gcttcaacgt cataagggtt ttgacttgga
    26581 acttaaaata cgaaaggaaa tgactcaaat cacggcttga gagctttctc tagccgagtg
    26641 atttgggcac taaaattatg ttagtatctt ataaatggtt aaaagaatta gtagatatcg
    26701 atgtgacacc agcagcactt gctgagaaaa tgtcgacaac gggtatcgag gtagaaggaa
    26761 ttgaagtccc tgcagatggt ttatcaaaac tggttgtggg gcatgtgctt tcttgtgaag
    26821 atgtgccaga gacacatttg catttgtgtc aggtagatac aggtgatgaa acgccacgtc
    26881 aaattgtgtg tggcgcacca aatgtgaaag cagggattaa ggttatcgtg gctgtgccag
    26941 gtgctcgtat tgcggataac tataaaatca aaaaaggcaa aatccgcggc atggaatctc
    27001 ttggcatgat ttgttccttg caagaacttg gtttgtcaga tagtattatt ccaaaagaat
    27061 tttcagatgg tattcaaatc ttgccagagg aggctgtgcc aggtgatgct atttttaaat
    27121 acctggactt ggacgatcat atcattgaat tgtccattac acctaaccgt gctgatgcgc
    27181 tttctatgcg aggtgtggca catgaagtcg cagctattta tggcaagtca gtatcattcc
    27241 cccaaaaaaa tctacaagaa tctgataagg caacttcaga agccattgaa gtggccattg
    27301 cctctgataa cgttctaact tacgctagcc gtgtcgttga aaatgtcaaa gttaagccaa
    27361 gtccacaatg gttgcaaaac cttttaatga atgctggtat tcgtcctatt aataatgtgg
    27421 ttgatgtgac caactatgtg ctcttgtatt ttggtcaacc tatgcatgct tttgattatg
    27481 ataaatttga agaccataag attgtggcgc gtgcggctcg tcaaggagaa agtttggtca
    27541 cacttgatgg tgaaaaacgg gatttgacca ctgaggatct tgttattacc gtggctgaca
    27601 aaccagttgc tttagcaggt gttatgggtg gtcaagccac tgaaattgat gccaactctc
    27661 agacggttgt tcttgaagct gctgtttttg atggcaaatc gattcgtaaa accagtggtc
    27721 gtttaaacct tcgttcggaa tcctcttcac gttttgaaaa aggggttaac tatgcaactg
    27781 ttttagaagc acttgacttt gcagcggcta tgttacaaga attagcagaa ggtcaagtct
    27841 tatcaggcca tgttcaagct ggacaattgc caacagaacc tgttgaagtc tcaactagcc
    27901 ttgactatgt gaatgttcgt ttaggaacag aattaacttt tgctgatatt caaaggattt
    27961 ttgaccaact tggatttggt ctaacaggag atgaaacaag cttcacggta gctgttcctc
    28021 ggcgtcgttg ggatgttagc atcccagctg acttggtgga agaaattgcg cgtatctatg
    28081 gttatgataa gttgccaaca accttaccag aggcaggggg aacggctgca gaattaacgc
    28141 caacacaggc tcttcgtcgt aaagtccgtg gcttagcgga aggattgggc ttgactgaaa
    28201 tcatttcgta tgctttgaca acaccagaga aagctgttga gtttgcagtg gcaccaagtc
    28261 acctgacaga actcatgtgg ccgatgtctg tggagcgttc agcactacgt caaaatatgg
    28321 tttcagggat gttagatacg gttgcctata atgtggctcg taaacaaagt aacttggctc
    28381 tttatgaaat tggaaaaatc tttgaacaag aagctaatcc aaaagaagac cttcctaatg
    28441 aagtgaacca ttttgctttt gctatttgtg gcttagtggc acaaaaagat ttccaaaccc
    28501 aagctcaggc agtggatttt taccatgcta aaggtaactt ggatacttta tttgcaaacc
    28561 ttaacctcaa ggttcaatat gtgccaacca aagacctagc aaacatgcac ccaggacgga
    28621 cagcgcttat cctattagat gagcaagtga ttggatttgt tggtcaagtt catccaggaa
    28681 cagcaaaggc ttatagtatt ccagaaacct atgtggcaga attggatatg gctgctttag
    28741 aagcagcgtt gccaagtgat caaacttttg cggaaatcac aaaattccct gctatgacac
    28801 gtgatgttgc cttgttactt gaccgtgagg tgagtcatca ggccattgta acagctattg
    28861 aatcagcagg tgtaaaacgc ttgactagca ttaaactctt tgacgtctat gaaggagcaa
    28921 ccattcaggc tggcaaaaaa tcaatggctt atagccttac tttccaaaat ccaaatgaca
    28981 atttaacgga tgaagaagtt gccaaataca tggagaaaat caccaaagca ttgacagaac
    29041 aagtcggtgc tgaagtacgc taagaaaaaa actgggcctc taaggctccg tttttttctg
    29101 ttataataga ggaaaaaaga actccagttc aaatgattgg agaaaaacat cagaaggtgg
    29161 aggaaaatca gaatgtatca aacaaaaata acaggcaacc gcctttacca cacggtttcg
    29221 aaaggctacg gagatagtgt gaccttattt ggaaaaactg atcaagggga aacccctatg
    29281 agtttactgg ttatcgcctt gtcttcttgt gtgactatgt gtgtgcaggg tttttaccat
    29341 cgtcaatttc agcaagacac acttgctgtt tctgttgagg ctagttatga ggaagaagtt
    29401 tttgaactac ttgttaggat tgatgatgtt ttagatgaca aaaagaaagc tgatttattg
    29461 gcttacattg gaacttattg tcgtgtgaaa caactcctca aacctgaagt tacggtgacc
    29521 attacgttgg aaggactgtc tcatgtctga tgctagtttg aaaacttacc aaaaacaatg
    29581 ggcttatcaa aagtattggg tcatggcaca ctcacagcag cattacaatg ctttaagaga
    29641 attgtttaaa ggaaatcagt ggtcagaaga aaaggtgttg acttttcatt gtttgattga
    29701 agaagcgcaa gccattcctc ccacagtaaa aagcttacga acggcatatc agcatgtttg
    29761 ggggtatttt aaaaaggtag cgagtcagga agaaaaagac catttcaaag atttggatgc
    29821 tcagttggaa actaaatctg aggagatgct atgcttttta caagagatga ctgctcatta
    29881 tcagccctct tatctgctat cttgccgttt gattaccaaa ggtccttaaa tgctaaacag
    29941 aaaagtaggg atttctggga agttatgcta agctagtgat ataaataagg gggaatgaga
    30001 gatgttttta gcgcttaatg aaatgaaaca atccaaattg cggtatggtt tgattgctgg
    30061 tttgctatgt ttggttgcct atttgatgtt ctttttgtca ggattggcct ttggtttgat
    30121 gcaggagaac cgctcggcgg ttgatctttg gaaagctgac agtgttttac tagccaagga
    30181 tgctgacgct accttgacct tatcacaggt gtccagagct caagaaaatc aaataacagc
    30241 agacaaggta gctcctcttg ctcaactcaa taccgtggcg tggtcagtta aaaatcctaa
    30301 ggatgccgac aaagttaagg ttagcctttt cgggattgat tctaatagct ttattcgtcc
    30361 taacattgta aaaggtcgat tatttaagac taacaaagag gttgttttgg atcaaagcct
    30421 tgcaaaagag gaagcttttg cgattggcaa ggacttttac acatcgagtt ctagtcaagc
    30481 attaactatc gttggttata ctcaaaatgc tagatttagt gttgcaccag tggtttatat
    30541 gaatttggaa gcttttgaaa cattaaaata tggagaacca ctaccaaaag ataagcaagt
    30601 tgttaatgct tttatcacta aaggaagttt aacagattat cctaaaaaag acttccaaaa
    30661 attagatatt aaaaccttta ttactaaatt acctggttat agcgctcaac ttttaacttt
    30721 tggctttatg attagttttc ttgtcattat ttcagctatt attattggta tttttatgta
    30781 tattttgact attcaaaagg cacctatttt tgggattatg aaagcgcaag gaatttctaa
    30841 caaaacgatt acgaccgctg tgcttatgca gacattcttt ttgagttttt taggtagtgg
    30901 tttagggttg ctaggtactt ggctgacatc attattatta ccaacagtag taccttttca
    30961 aagcaattgg tttttgtatt tggctatatt cgttagtatg atctgttttg ctctattagg
    31021 caccttattt tctgttttta acattatacg aattgatcct ttgaaagcaa ttggatagga
    31081 ggtagtgatg tcggttttaa catttaaaca agtgaccaaa acatttcaag atggtcatca
    31141 cgaaatcaac gcattaaagg caactgactt ttccattgaa gctggagaat ttgtggctat
    31201 tattggacca tcaggttctg gaaaatcaac ctttttgacc attgctggtg gcttacagac
    31261 cccaagttct ggtcagctaa tcattgatgg cactgattat acgcatttat cagaaaaaga
    31321 gcgctcacgc ttgcgcttca aatccgttgg ttttatctta caagcttcca atctgatacc
    31381 ttttttaacg gtccagcagc agttggaatt ggtcgatcat ttgacaggaa gcaaagaaaa
    31441 agccaaagcc aatcagttat ttgacgattt aggcattaca gggctaaaac atcagttacc
    31501 tcaagaatta tccggcggag aaaggcaaag agcggctatt gcccgtgctc tttaccatga
    31561 tccagcactg atattggcag acgaacctac tgcaagtttg gatactgaaa aagcctatga
    31621 agtggtcaaa ctgttggcta aggaaagtaa agaaaaaaac aaggccatta ttatggtcac
    31681 tcatgatgat cggatgctaa aatattgtga caaagtctat cggatgcaag atggagagtt
    31741 atgtcaagag agatagtaga ctggtaagag tttatgatat tagaaaaaaa ccaaacaagc
    31801 acagcacttg tttggttttt ttctaagtgt gactattatc aatacgtcac catttcccta
    31861 aagcttacta tacgggatgc taaaggtatc accatgctca aagtcgccat attggtaacc
    31921 tcggtcaaac caacgttggc gttgcttaga agtgccatgt gtaaaactat caggaacggt
    31981 tctgccatag gtttcttctt gcagagtatc atccccaact gcgtgagctg ctgccattgc
    32041 ttcctcaata tctccttttt ccaaaaggcc ttgcccttga acataattag cccaggcacc
    32101 agcatagtaa tcagattgta attccaactt gacgttgagc tgattggctt tagcctttga
    32161 ttttccttgt ctagcgctag cgtaattatc cataattccc aattcatttt ggatgtggtg
    32221 gccaacttca tgagcaatca cataggccat agcaaaatca ccttttgcgc cgtacttggt
    32281 tgatagttca ttataaaaag aaatatctaa atacactttt tgatcgcctg ggcaataaaa
    32341 aggaccagaa gaggcttgcc ccctgccaca agcggtttga gttgctccag tatatagaac
    32401 taatgttggt ttatgataag ttaacccttt ctcacgaaat gtcttagtcc aatagtcctc
    32461 ggtagaggcg aagaccttac tgacaaaaga aacctgttct tgactagcct tgtcaccatt
    32521 tgtacgcgtt acttggcttg attgataggc attgttgttg gtagaagaag gcttgcctcc
    32581 taaaacacca gataggccac cacctcccat aactaggaga aggagaagaa taaccagctt
    32641 ggttttccaa cctcctcgtg aaaagagtaa ttgaagcagg agaccgcccc ctaggccgcc
    32701 gcccccaaaa gaaccagacg attgaccacg tctatcttca acctgttgac tttctctcaa
    32761 atcatccgtt ttcataagtt tctcccatct atttcttgat tttattataa aataattcta
    32821 ttagaattgc tatttttatg atgagattat aactaaaaat aggaaaagca ataggtattt
    32881 gctataatat aacaagggta gattcagttt gtataaagga gtcatatgaa attaatttac
    32941 acagagatga gttactcgat gacagagatt ttggtgaatg aagcacgaaa agctgctgac
    33001 caaggttatc gagtgttcta tattgctcca aattctcttt catttgaaaa ggaaagggaa
    33061 gtcttaacct tattgcctga acgagggact ttttcgataa tagtcacccg gttcgttcaa
    33121 atgtcaaggt attttacagt tgagagctct ccttctaagc aacatctaga tgacacgact
    33181 ttggctatga ttttttatcg ggccttgatg caattaaaac cagaagattt gccgagttat
    33241 ggtcgtttgc aaaataattc ggtttttatt gaacaactag ttgagttata taaagaatta
    33301 aaaaacgcac aattaagcgt acatgattta acagggttag accatcctca aaaacaagaa
    33361 gatcttatta aaattattga gcttgcagag acgatcatga ttcagcaaga ttataatcaa
    33421 gatagtcctc tacaatcttt tgcaagagca attaagttag gacttttaaa taaccaacta
    33481 tcaaaaacag ttgttgttat tgatggtttt tcacgttttt cagctgaaga ggattattta
    33541 ttatcacttt taaataacaa ctgtcaagag gtcatcattg gcagttatgt gagtcaaaaa
    33601 gcttatcaaa aatcttttat taagggaaat atctatgagg ctagccttca ttttttgcaa
    33661 gatttggctc aaaaatacca tatcaagccg gtatttgcga cctctaatca agtgtttaaa
    33721 ccagcctttt cacggctaac acaattattt gaagcaactc atgatttttc acaagtagac
    33781 tggcaactcc aaaagagtga tcttgatcac tttagccttt ggcaatgtca tcatcaaaaa
    33841 gaggaaattg aacatgtagc aaaatccatt cggcaaaagc tatatgaagg ttaccgttat
    33901 aaggatatct tagtgttgct aggagatatg gatgcttatc aattacaaat tgggcctatc
    33961 tttgataagt ttgaaatccc ctactattta ggaaaagcag agcctatggc tgcccatcca
    34021 ttagtccaat ttatagaatc gctcgaacga agtcagcgct ataattggcg tcgtgaagac
    34081 attttaaaca tgttaaaatc aggacttttt ggttgttttg atgatagtga tattgatcgc
    34141 tttgaggaat atactcagtt tgcagatatt aaaggattta ctaaattttc aaagcctttt
    34201 accattaact cttctaggca gtatcctctt gactttttaa atgaaatgag acaagacatc
    34261 gttttacctt tgcaagaatt gtttaagagt caaaaacaac ttggtgcttc acttgttgac
    34321 aaattaatac tttttttaaa aaagattaga ttagctgaga atatgcaagg tttagcacag
    34381 tcccaattag aggtggaaaa aaacgaagaa gtttggaaaa ggtttacaga tattttgact
    34441 tcttttcatc atatctttgg ccaagaaaaa ttacggctat cagattgcct tgctttaatt
    34501 aaaacgggaa tgaaatcagc tcagtatcgt gttgttccag cgacattaga tgttgtcact
    34561 attaaatctt atgaccttgt tcaaccacat tcaaaacctt tcgtttatgc aattggtcta
    34621 actcaatcac attttcctaa gcaaattcac cattcaggac tactttctga tcaagagcgt
    34681 gctagaatta atgaaataag aaattatcgt cattttgata ttgctagtgc cgaaaatagt
    34741 aaaaaaaacc atcaaactgc gttatctctg tttaatgctg ctactaagga gttagttctt
    34801 agtgtttcaa cggtaatcaa cgagacattc gatgatctat ctccttatct taaagaatta
    34861 ataaactttg ggttacctct tttggataaa ggcaaaaact atctatctta tgataatagc
    34921 gatattggta actataaagc tctcttgtca caaattattg ctattaaccg acaagatctt
    34981 atagaaatgt cagatcaaga taagatgttt tggactgttg tcttacgtta tctcaggaaa
    35041 cagttaagaa agcaacagct agagctacct acttcagatt atcgtttatc aacaaagcct
    35101 ttgtctaaag aagttatcga ggtatgtttt ccaaagggaa tccctttaaa gctatctgca
    35161 actgctttaa ccgtttttta taataatcag tacaactatt ttttgaaata tgttttgaat
    35221 ttaaacaaaa cagaatccat tcatccagat tccagaattc atggtcaata cttgcatcgt
    35281 gtctttgagc ggttaatgaa agatcacacg caagaaccgt ttgataataa gttaaaacaa
    35341 gcaatctacc ataccaatca agaaagtttt tttcagcagg tttaccaaga taatgccgag
    35401 gctgagtaca gtttagctat actagaagat attgttagat caacagcacc tatcttgcaa
    35461 cttaaccaaa acatacaggt cattgatcaa gaaaagaact ttcagctgga tatgggaaat
    35521 gagatattag tccacgggat cattgatcgt attgaccagc tgagcgacgg tagcttgggc
    35581 attgttgact ataaatcaag cgcgaatcaa tttgatattg ggacctttta taatgggttg
    35641 agtccacaac taatgactta cctcgcagcc ttaaaacaga ttgcaccgca cgatataaac
    35701 caattatttg gggctatgta ccttcatttg caagatccta aattggatct agtaacattt
    35761 aaacaaattg ataacacatt agtggagagt atctataaag cgttgaccta taaaggtatt
    35821 ttttcagaag tcgaaaaaga gcatctatct actggtgctt accaaaccaa aaatgctctt
    35881 tattccaatg acgaattgga aacactttta aattataaca agtacctcta tcttaaagct
    35941 gctaaacaca ttaaaaaggg acattttttg attaatcctt atacaagcga tggtaagaca
    36001 gttcaaggag atcaattaaa agccattacc cgttttgaag cagatcttga catgggacag
    36061 gcaaggcgac tagtgactct ccctgctaag gagaaaaaag agtgcttttt aacattaatg
    36121 agaaaggaga gccacttgtg atttcttttg ccccattttt aagccccgaa gctattaaac
    36181 atttgcaaga aaacgaaagg tgcagagatc agtctcaaaa acgcacagct caacaaattg
    36241 aagcaattta tactagtggc caaaatatac ttgtatcagc ttctgctggt tcaggaaaaa
    36301 cctttgtaat ggtcgaacgc atacttgata aaattttgag aggtgtttca attgatcggc
    36361 tttttatctc aacctttact gttaaagcag ctacagaact gcgtgagcgg attgaaaaca
    36421 aattatactc acaaattgct caaactacag attttcaaat gaaagtttat ttaacagaac
    36481 aattgcaatc tctttgtcaa gctgatattg gtactatgga tgcttttgca cagaaagtag
    36541 taagtcgcta tggttatagc attggcattt catcccaatt tcgtatcatg caggataaag
    36601 cagaacaaga tgttttaaag caagaggtgt ttagcaaact ctttaatgag tttatgaatc
    36661 aaaaagaggc accggtgttt agggctcttg tgaaaaattt ttctggtaac tgtaaagaca
    36721 cttcagcttt tagagagtta gtttatactt gttattcttt tagccaatcg acagaaaacc
    36781 caaaaatatg gttgcaagaa aattttctaa gcgctgctaa aacttaccaa agacttgaag
    36841 atatcccgga tcatgatatt gaactcttac ttttggcaat gcaagacact gcaaatcagc
    36901 taagagatgt gactgatatg gaagattatg ggcagctgac taaggcaggt agccgatctg
    36961 ctaaatacac taaacactta acgatcatag aaaagttgtc tgattgggtg cgtgatttta
    37021 aatgtttgta tggaaaagcc ggattggatc ggttgatcag agatgtgaca ggccttatac
    37081 catctgggaa tgatgttaca gtctcgaagg taaaataccc tgtttttaag accttgcatc
    37141 aaaaattaaa acaatttagg catttagaaa caattttaat gtatcagaaa gactgttttt
    37201 ccttattgga acagttacaa gattttgtgc ttgcgttttc agaagcttat ttagctgtca
    37261 aaatacaaga aagtgctttt gaattttcag atattgcaca ctttgcaatc aaaattttag
    37321 aggaaaatac ggatattcgc caatcctatc agcaacacta tcatgaggtg atggttgatg
    37381 aatatcaaga taacaatcat atgcaagagc gactcctgac cttactatcg aacggtcata
    37441 atcgctttat ggtaggagat atcaaacaat cgatctatcg atttcggcaa gccgatcctc
    37501 agatttttaa tcaaaagttt agagactatc aaaaaaaacc tgagcagggg aaagtgattt
    37561 tactcaaaga aaactttcgt agccaatcag aggtgttaaa tgtcagcaat gctgttttta
    37621 gtcacttaat ggacgaatca gtaggagacg tcttatacga tgagcaacat cagttaatag
    37681 caggtagtca tgctcaaaca gtcccctatc tagaccgtcg tgctcagtta ttgctatata
    37741 atagcgataa agatgatggc aacgcccctt cagatagtga gggtatttca tttagtgagg
    37801 ttacaattgt tgccaaagaa attattaagc ttcacaatga taagggtgtc ccttttgaag
    37861 acattacgtt actcgtttct tcaagaacaa gaaatgatat catttctcat acattcaatc
    37921 aatatggtat tcctatagta acagatggtg ggcagcaaaa ctatcttaaa tctgttgaag
    37981 tgatggttat gttagataca ttacgcacca ttaataaccc aagaaatgat tatgcccttg
    38041 tggctttact gcgctcaccg atgtttgcct ttgatgagga tgatttagca agaatagcac
    38101 ttcaaaaaga caatgagcta gataaagatt gcctatatga caagatacaa agggctgtga
    38161 ttggaagagg tgctcatcct gaattgattc acgatacctt gcttggcaag ttaaatgttt
    38221 ttttaaagac gttgaaaagc tggcgtcgat acgctaagct agggtcgttg tatgacttga
    38281 tttggaaaat ttttaatgat cgtttttatt ttgattttgt agctagtcaa gcaaaagcag
    38341 aacaagcaca agctaatcta tacgcattag ctctacgtgc taatcagttt gaaaaatcgg
    38401 gctataaagg gctataccgt tttattaaaa tgattgataa ggtacttgag acgcaaaatg
    38461 acttagctga tgtggaagtg gctactccta aacaagctgt taatttaatg accattcaca
    38521 agtctaaagg tttacaattt ccgtatgtat ttatccttaa ttgtgacaag cgcttctcaa
    38581 tgacagatat tcataaatca tttattctga atcggcagca cggtatcggt atcaagtacc
    38641 ttgcagatat caaaggttta cttggtgaaa caacactcaa ttctgttaaa gtaagcatgg
    38701 aaaccttacc ttatcaattg aacaaacaag agttgcgctt agcaacttta tcagaagaaa
    38761 tgcgcttact gtatgttgct atgacacgag ctgaaaaaaa agtttatttt attggtaaag
    38821 ctagtaagag caaaagtcaa gaaatcacag atcctaaaaa gttaggcaaa cttttgccgc
    38881 tggctttacg agaacagtta ttgacattcc aagattggct attagcaata gcagatatat
    38941 tttcaactga agatctttat tttgatgttc gctttattga agatagtgat ttgacacaag
    39001 agtcagtcgg acgacttcaa acaccacagt tattaaatcc agatgatctt aaagataatc
    39061 gtcaatcaga aacaattgca cgggctttag atatgttaga agcagtgtct caattgaatg
    39121 ccaattatga agcagctatt catttgccaa cagttcgaac gcctagccaa cttaaggcaa
    39181 cttacgagcc tttattagaa cccattggtg tagatattat agagaaatct tctcgatcgc
    39241 tatctgattt tactttgcca catttttcaa aaaaagcaaa agttgaagca agtcatattg
    39301 gatcagctct tcatcagttg atgcaggtgc tccctttgtc aaaaccgata aatcaacaaa
    39361 cgcttttaga cgctttaaga ggaattgata gtaacgaaga ggtaaaaaca gctcttgatc
    39421 tcaaaaaaat agagtcgttc ttttgtgata caagcctagg ccaatttttt cagacttacc
    39481 aaaaacactt gtatcgagaa gcgccatttg ctattttaaa acttgaccct atcagtcaag
    39541 aagagtatgt cctacgtggt attatagatg cctacttttt gtttgatgat catattgtat
    39601 tagtggacta taaaacagat aaatacaagc agcccattga gttaaaaaag cgttaccaac
    39661 aacagttgga gttatatgca gaagctctca ctcaaacgta taaacttcct gtgactaagc
    39721 gctatcttgt tttaatggga ggtggaaagc cagaaattgt cgaagtttaa ggttgttgtg
    39781 acacaagggt attttgcaaa tgataatagt atagttgagg attttacgaa atgcttgaaa
    39841 agc
//
LOCUS       DAXWIQ010000014        36359 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000014 DAXWIQ010000000
VERSION     DAXWIQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36359
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.014"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            73..600
                     /locus_tag="ACGEUI_000703"
     CDS             73..600
                     /locus_tag="ACGEUI_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFF8165559.1"
                     /translation="MSIDDYRPTYMVEAIYDLRANDLLRHGITAVLVDLDNTLIAWNN
                     PDGTPEVRAWLDEMTIADISVVVVSNNKHSRVERAVSRFGVDFISRALKPFAYGIEKA
                     IARYGFDRNEVIMVGDQLMTDIRASHRAGIKSVLVKPLVASDAWNTKINRWRERRVMA
                     KLEEKYGKLSYQKGI"
     gene            600..1718
                     /gene="yqeH"
                     /locus_tag="ACGEUI_000704"
     CDS             600..1718
                     /gene="yqeH"
                     /locus_tag="ACGEUI_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFF8165560.1"
                     /translation="MEELFCIGCGIQIQTEDKEKAGFTPAAALKKGMETGELYCQRCF
                     RLRHYNEITDVHITDDEFLRLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFISGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPLDVMLTSAQNKYAIKDLIGRINEL
                     RNGRDVYVVGVTNVGKSTLINAIIQEITGNKDVITTSRFPGTTLDKIEIPLDDGTFIF
                     DTPGIIHRHQMAHYLSPKELKIVSPKKEIKPKTYQLNPEQTLFLGGLARFDFINGERQ
                     GFTAFFDNQLELHRTKLAGADAFYDKHVGTLLTPPDKKELTAFPKLVRHEFTIDQKMD
                     IVFSGLGWIRVNGQKDSKAIVAAWAPEGVAVIVRKAII"
     gene            1743..2051
                     /gene="yhbY"
                     /locus_tag="ACGEUI_000705"
     CDS             1743..2051
                     /gene="yhbY"
                     /locus_tag="ACGEUI_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFF8165561.1"
                     /translation="MLTSKQRAFLKSEAHSLKPIVQIGKNGLNDHIKTSIRQALDARE
                     LIKVTLLQNTDEDIHEVAEILEEEIGCDTVLKIGRILILYKVSAKKENRKLSPKVKAI
                     "
     gene            2120..2752
                     /locus_tag="ACGEUI_000706"
     CDS             2120..2752
                     /locus_tag="ACGEUI_000706"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFF8165562.1"
                     /translation="MALELLTPFTKVELEEEKKESNRKQIGILGGNFNPIHNAHLVVA
                     DQVRQQLGLDQVLLMPECKPPHVDAKETIDEKHRLRMLELAIEDVEGLAIETCELERQ
                     GISYTYDTMLYLTEQHPDVDFYFIIGADMVDYLPKWHRIDELVKLVQFVGVQRPKYKA
                     GTSYPVIWVDLPLIDISSSMIRDFIKKGRQPNYLLPKRVLDYITQEGLYQ"
     gene            2749..3342
                     /gene="yqeK"
                     /locus_tag="ACGEUI_000707"
     CDS             2749..3342
                     /gene="yqeK"
                     /locus_tag="ACGEUI_000707"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFF8165563.1"
                     /translation="MTYEDYLPYSRTELLAKIAEQMSPKRFKHVLGVEKAALSLAECY
                     GCNPDKAGLAALLHDYAKECPDQVFLDLIDKYQLSPELAKWNNNVWHGMVGIYKIQED
                     LGLKDKDILRAIEIHTVGAAEMTLLDKVLYVADYIEEGRIFPLVDDARKIAKLDLNQA
                     VAYETVNTVAYLASKAQPIFPQTLDTYNAFCSYLKEY"
     gene            3366..3719
                     /gene="rsfS"
                     /locus_tag="ACGEUI_000708"
     CDS             3366..3719
                     /gene="rsfS"
                     /locus_tag="ACGEUI_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFF8165564.1"
                     /translation="MKKEELLKIVVEATEEKRAKDILALDLEGLTSLTDYFVIASATN
                     SRQLEAIADNIREKVKEAGGDASHVEGNSQAGWVLLDLTDVVVHLFLEDERYHYNLEK
                     LWHEAPAVALDAYLA"
     gene            3767..4510
                     /locus_tag="ACGEUI_000709"
     CDS             3767..4510
                     /locus_tag="ACGEUI_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8165565.1"
                     /translation="MEHNYEKFASVYDAVMDDSLYDLWTDFSLRHLPKSKGRNRLLEL
                     ACGTGIQSVRFAQAGFDVTGLDLSQDMLAIAKKRAQSAKKKIDFIQGNMLDLSQVGQF
                     DFVTCYSDSICYMQDEVDVGDVFKEVYDVLANDGIFIFDVHSTYQTDECFPGYSYHEN
                     ADDFAMVWDTYADEAPHSVVHELTFFIQEDDGRFSRFDEVHEERTYELLTYDILLEQA
                     GFKSFKVYADFEDKEPTKTSKRWFFVAYK"
     gene            4764..5870
                     /locus_tag="ACGEUI_000710"
     CDS             4764..5870
                     /locus_tag="ACGEUI_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFF8165566.1"
                     /translation="MTVTGIIAEFNPFHNGHKYLLETAEGLKIIAMSGNFMQRGEPAL
                     IDKWIRSEMALKNGADIVVELPFFVSVQSADYFAQGAIDILCQLGIQQLAFGTENVID
                     YQKLIKVYEKKSEQMTAYLSTLEDTFSYPQKTQKMWEIFAGVKFSGQTPNHILGLSYA
                     KASAGKHIQLCPIKRQGAAYHSKDKNHLLASASAIRQHLNDWDFISHSVPNAGLLINN
                     PHMSWDHYFSFLKYQILNHSDLTSIFQVNDELASRIKKAIKVSQNIDHLVDTVATKRY
                     TKARVRRILTYILVNAKEPTLPKGIHILGFTSKGQAHLKKLKKSRPLITRIGAETWDE
                     MTQKADSIYQLGHQDIPEQSFGRIPIIIKNERLN"
     gene            6352..7068
                     /locus_tag="ACGEUI_000711"
     CDS             6352..7068
                     /locus_tag="ACGEUI_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8165567.1"
                     /translation="MGRKWANIVAKKTAKDGATSKVYAKFGVEIYVAAKQGEPDPELN
                     TALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADSVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            7271..8113
                     /locus_tag="ACGEUI_000712"
     CDS             7271..8113
                     /locus_tag="ACGEUI_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8165568.1"
                     /translation="MIKKQYLGRATIALASTLVLAACGSSKTAESGNQGSSKEVLFAT
                     VGTTAPFSYEKGGQLTGYDIEVAKAVFKGSDDYKVSFKKTEWSSIFTGLDSGKYQMGG
                     NNISFTKERSAKYLFSYPIGSTPSVLVVPKDSDIKSFDDIQGHTTQVVQGTTSVAQLE
                     DFNKKHSDNPVTLKFTNENITQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIE
                     LTSTEQPFIYFIFSQDQEKLQSFVNKRIKELTADGTLSKLAKEHLGGDYVPSDKELKL
                     PTAN"
     gene            8442..9287
                     /locus_tag="ACGEUI_000713"
     CDS             8442..9287
                     /locus_tag="ACGEUI_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8165569.1"
                     /translation="MSFKKWIGIVGIALASTVLVACSEKQDDKNTLTIGVMTKTESDQ
                     ARWDKVEELLKKDNITLKYKEFTDYSQPNKAVANGEVDINAFQHYNFLNNWNKENKEH
                     LVAIADTYISPINLFSGTSQDGKAKYKSVADLPNGTQIAVPNDATNESRALYVLQSAG
                     LIKLNVSGDQLATIANISENKKKLDIKELDASQTARALVSADAAVVNNSYAVPAKIDY
                     KTSLFKEKADDNSKQWINIIAGQKDWEKSEKADAIKKLIKAYQTDEVKKVVEKTSNGI
                     DVSVW"
     gene            9537..10601
                     /locus_tag="ACGEUI_000714"
     CDS             9537..10601
                     /locus_tag="ACGEUI_000714"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFF8165570.1"
                     /translation="MNEAIIQLDHIDITFRQKKRVIEAVKDVTVHINQGDIYGIVGYS
                     GAGKSTLVRVINLLQAPTNGKITVDGDVTFDQGKIQLSADALRQKRRDIGMIFQHFNL
                     MAQKTAKENVAFALRHSSLSKTEKEHKVIELLELVGLSERADNYPAQLSGGQKQRVAI
                     ARALANDPKILISDEATSALDPKTTKQILALLQELNRKLGLTIVMITHEMQIVKDICN
                     RVAVMQNGVLIEEGSVLDIFSNPKEALTQEFITTATGIDEALEKINQQDIVKHLPANA
                     LLAQLKYAGTSTDEPLLNSIYRQFEVTANILYGNIEILDHIPVGDMIVVLEGQAENIL
                     AAEKALHEAGVDVSILKRGA"
     gene            10602..11294
                     /locus_tag="ACGEUI_000715"
     CDS             10602..11294
                     /locus_tag="ACGEUI_000715"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFF8165571.1"
                     /translation="MSQLIQTYLPNVYELGWSGDAGWGLAIWNTLYMTIVPFIVGGAI
                     GLLLGLLLVLTGPDGVIENKTICWVIDKVTSIFRAIPFVILIAILASFTYLLLRTTLG
                     ATAALVPLTFATFPFYARQVQVVFSELDKGVIEAAQASGATFWDIVKVYLSEGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGNVAISYGYNRFNNDVTWVATIIILLIIFAIQF
                     IGDSLTRRFSHK"
     gene            complement(11348..12718)
                     /gene="brnQ"
                     /locus_tag="ACGEUI_000716"
     CDS             complement(11348..12718)
                     /gene="brnQ"
                     /locus_tag="ACGEUI_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFF8165572.1"
                     /translation="MKQKNVYIVIGFMLFALFFGAANLIYPAFLGIYSGHQILWSIIG
                     FCLTGVSLPLLGVIAVAKSGSGDVESLARPISKWYAIFYSSILYLSIGPFFAIPRTGA
                     TSFSVGIAPILGDNTTNKAIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISIL
                     VIASFVHPAGNYGDAFNAGVGVNNAFKDFPFVAGLIQGYGTMDALASLVFAILVIEAT
                     KQFGAKTDKEMTKITLISGAIAILLLALVYIFVGRIGATSQSLFPFIDGSFTLHGNPV
                     NGGQILSHASRFYLGGIGQAFLAVVIFLACLTTSTGLITSSAEYFHKLVPALSHIAWA
                     TIFTLLSAFFYFGGLSVIINWSAPVLFLLYPLTVDLIFLVLAQKCFNNDPIVYRTTIG
                     LTFIPAIFDALLTLSQMTGLFHLPEAVVTFFQKTVPLGQFSMGWIIFAAIGFLIGLIL
                     SKTKKS"
     gene            12952..14166
                     /gene="sstT"
                     /locus_tag="ACGEUI_000717"
     CDS             12952..14166
                     /gene="sstT"
                     /locus_tag="ACGEUI_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFF8165573.1"
                     /translation="MKKIYDLWVRVSLIKKIGIGVVIGVMLGILAPDLTGFSILGKLF
                     VGGLKAIAPLLVFALVSQAISHQKKGKQTNMTLIIVLYLFGTFASALVAVLTAYLFPL
                     TLVLNTPVNTELSPPQGVAEVFQSLLLKLVDNPINALATANYIGVLSWAIIFGLALKA
                     ASKETKHLIKTAAEVTSQIVVWIINLAPIGIMSLVFTTISENGVGILSDYAFLILVLV
                     GTMLFVALVVNPLIAVLITRQNPYPLVLRCLRESGLTAFFTRSSAANIPVNMQLCQKI
                     GLSKDTYSVSIPLGATINMGGAAITINVLTLAAVHTFGIPIDFLTALLLSVVAAVSAC
                     GASGVAGGSLLLIPVACSLFGISNDLAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     IAENAFWKRKKA"
     gene            complement(14221..14895)
                     /gene="ktrA"
                     /locus_tag="ACGEUI_000718"
     CDS             complement(14221..14895)
                     /gene="ktrA"
                     /locus_tag="ACGEUI_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium uptake transporter gating subunit
                     KtrA"
                     /protein_id="HFF8165574.1"
                     /translation="MLKRKTVGVLGLGIFGRTVARELSNFDQDVIAIDIRESHVKEVA
                     DLVTKAAVGDITDKEFLLAVGIEHCDTVVIASGNNLESSVLAVMHCKKLGVPTIIAKA
                     KNKIFEEVLYGIGATKVITPERDSGKRVASNLLRRHIESIIYLEHGISMIEFVIPKSW
                     EGQSLSELDVRRKYELNVIGMRQKEVKTLDTNVKPFEPLEPNTIIVAIANDHTFEKFD
                     YLGYLK"
     gene            complement(14905..16281)
                     /gene="ktrB"
                     /locus_tag="ACGEUI_000719"
     CDS             complement(14905..16281)
                     /gene="ktrB"
                     /locus_tag="ACGEUI_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium uptake transporter channel subunit
                     KtrB"
                     /protein_id="HFF8165575.1"
                     /translation="MKRSFIKSLSVTQRLTFSFAIVILIGTLLLSMPFTHYQNGPNTV
                     YLDHFFNVVSMVCVTGLSVVPVAEVYNGIGQTIAMALMQIGGLGLVTLIAVSTFALKR
                     KMRLSDQTLLQSALNRGDSKDLKHYLFFAYKVTFSLEAFAAIVIMIDFIPRFGWKNGI
                     FNSIFLAVSAFCNAGFDNLGSSSLKDFMLNPTLNVIITFLIISGGLGFAVWVDLGVAF
                     KKYFFEKPHCYGATFRKLSNQSRLVLQTTAVILFLGTFLTWFLEKDNSKTIANFSLHQ
                     QLMVSFFQTVTMRTAGFATISYNDTLAPTNILYMIQMVIGGAPGGTAGGIKVTTAAIT
                     FLLFKAELSGQSEVTFRNRIIANKTIKQTMTVLIFFFAVLMIGFILLLSVEPHIAPIP
                     LLFESISAIATVGVSMDLTPQLSTAGRLIVIVLMFVGRVGPITVLISLIQRKEKTIQY
                     ATTDILVG"
     gene            complement(16366..17079)
                     /gene="rsmG"
                     /locus_tag="ACGEUI_000720"
     CDS             complement(16366..17079)
                     /gene="rsmG"
                     /locus_tag="ACGEUI_000720"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFF8165576.1"
                     /translation="MTPQDFYRTLEEDGFSLSSKQKEQFDTYFKSLVEWNTKINLTAI
                     TEENEVYLKHFYDSIAPILQGFLANEPIKLLDIGAGAGFPSLPMKILFPNLEVTIIDS
                     LNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELT
                     IPFLKIGGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKK
                     KETPNKYPRKAGLPNKKPL"
     gene            17229..17786
                     /locus_tag="ACGEUI_000721"
     CDS             17229..17786
                     /locus_tag="ACGEUI_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFF8165577.1"
                     /translation="MPTVLIILVVLGVLALWLMISYNSLVKSRMHTKEAWSQIDVQLK
                     RRNDLIPNLIETVKGYASYEQKTFEKITDLRARVANASTPQETMAASNELSKQVTSLF
                     AVAENYPDLKANENFLKLQEELTNTENKISYSRQLYNSTTSNYNLQLESFPSNIAGKL
                     FGFKPSEFLQTPEAEKEVPKVEFNF"
     gene            17833..18729
                     /gene="htpX"
                     /locus_tag="ACGEUI_000722"
     CDS             17833..18729
                     /gene="htpX"
                     /locus_tag="ACGEUI_000722"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HFF8165578.1"
                     /translation="MLYQQISQNKQRTVVLLVVFFALLALIGASAGYLLLDNYAMGLV
                     LALVIGVIYATSMIFQSTSLVMSMNNAREVTEKEAPGFFHIVEDMAMVAQIPMPRVFI
                     IEDPSLNAFATGSSPQNAAVAATTGLLEVMNREELEGVIGHEISHIRNYDIRISTIAV
                     ALASAVTVISSIGGRMLWYGGGSRRQRDDGDDDVLRIITLLLSLLSLLLAPLVASLIQ
                     LAISRQREYLADASSVELTRNPQGMIKALEKLQLSQPMKHPVDDASAALYINEPRKKR
                     SFSSLFSTHPPIEERIERLKNM"
     gene            18963..19496
                     /locus_tag="ACGEUI_000723"
     CDS             18963..19496
                     /locus_tag="ACGEUI_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFF8165579.1"
                     /translation="MLAISEIRKHPDGLSFDRLCDVKSMLLERDQQIIDIKAVKAVGN
                     VCYDKGLYLLDYQLSYEVILPSSRSMVPVCLSEVQHIQELFIEATDLADKKELVEDNL
                     VLVLDKDAINLEESIVDNILLAIPLQVLTEEEKKSKELPAGQNWAVLTEEDYQCLKEE
                     KQKENNPFASLQGLFDE"
     gene            19763..20449
                     /gene="covR"
                     /locus_tag="ACGEUI_000724"
                     /gene_synonym="csrR"
     CDS             19763..20449
                     /gene="covR"
                     /locus_tag="ACGEUI_000724"
                     /gene_synonym="csrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CovR"
                     /protein_id="HFF8165580.1"
                     /translation="MTKKILIIEDEKNLARFVSLELQHEGYEVIVEVNGREGLETALE
                     KEFDLILLDLMLPEMDGFEVTRRLQTEKTTYIMMMTARDSIMDVVAGLDRGADDYIVK
                     PFAIEELLARIRAIFRRQDIESEKKVPSQGIYRDLVLNPQNRSVNRGDDEISLTKREY
                     DLLNILMTNMNRVMTREELLSNVWKYDEAVETNVVDVYIRYLRGKIDIPGKESYIQTV
                     RGMGYVIREK"
     gene            20455..21957
                     /gene="covS"
                     /locus_tag="ACGEUI_000725"
                     /gene_synonym="csrS"
     CDS             20455..21957
                     /gene="covS"
                     /locus_tag="ACGEUI_000725"
                     /gene_synonym="csrS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CovS"
                     /protein_id="HFF8165581.1"
                     /translation="MENQKQKQKKYKNSLPKRLSNIFFVLFFCIFSAFTLIAYSSTNY
                     FLLKKEKQSVFQAVNIVRVRLSEVDSNFTLENLAEVLYKNDKTHLRIDDRKGSRVIRS
                     ERDITNTLDANQDIYVYNIDKQMIFTTDNEESSPGLHGPIGRVYHDHIEDQYRGFSMT
                     QKVYSNRTGKFVGYVQVFHDLGNYYVIRARLLFWLLVVELFGTSLAYLIILITTRRFL
                     KPLHNLHEVMRNISENPNNLNLRSDISSGDEIEELSVIFDNMLDKLETHTKLQSRFIS
                     DVSHELRTPVAIIKGHIGLLQRWGKDDSDILEESLTATAHEADRMAIMINDMLDMVRV
                     QGSFEGHQNDMTVLEDSIETVVGNFRVLREDFIFTWQSENPKTIARIYKNHFEQALMI
                     LIDNAVKYSRKEKKIAINLSVTGKQEAIVRVQDKGEGISKEDIEHIFERFYRTDKSRN
                     RTSTQAGLGIGLSILKQIVDGYHLQMKVESELNEGSVFILHIPLAQSKES"
     gene            22172..22666
                     /gene="nrdR"
                     /locus_tag="ACGEUI_000726"
     CDS             22172..22666
                     /gene="nrdR"
                     /locus_tag="ACGEUI_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFF8165582.1"
                     /translation="MRCPKCNYHKSSVVDSRQAEDGNTIRRRRECEQCHTRFTTFERV
                     EELPLLVIKKDGTREQFSRDKILNGVVQSAQKRPVSSTDIENVISRIEQEVRTTYENE
                     VSSTAIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITNRVRGKKKRLNN
                     DETN"
     gene            22650..23825
                     /locus_tag="ACGEUI_000727"
     CDS             22650..23825
                     /locus_tag="ACGEUI_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFF8165583.1"
                     /translation="MMKPIDTFTYLKRNKVTLDSVTLIQLYFPIIGSDAVSIYQYFIH
                     FFDDGLQRHKFSDILNHLQFGMKRFEDALAILTAMELVSVYQLSDTYLITLHQPLSRD
                     LFFQHPAYSRLLEQKIGEVAVSELQVTVPSQARNISKRFSDIFGVQGDLTNVPQKPQK
                     NFDLSSFQQLMVRDGLQFEDNQKDIISLYSIAEQYDMTWFDTYQIAKATAVNGKIRPE
                     RLLAKKNQSMTKPSKENFSQAEQIILREAKQDSALVFLEKIKKARRATITKDERILLQ
                     TLAKMNFLDDVINVMVLYTFNKTKSANLQKSYVLKMANDFAYQKVSTAEEAIVVLRAF
                     TDRQSRRQSKVKTSQSNVPKWSNPDYQETTSQEEQAKLDQFKQAALKRLENLGKGGD"
     gene            23826..24728
                     /gene="dnaI"
                     /locus_tag="ACGEUI_000728"
     CDS             23826..24728
                     /gene="dnaI"
                     /locus_tag="ACGEUI_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFF8165584.1"
                     /translation="MEKIGETMAKLGQNTRVNSDQLIQTILADPEVASFISQHHLSQE
                     QINLSLSKFNQFLVERQKYQLKDPSYIAKGYQPILAMNEGYADVSYLETKELVEAQKQ
                     AAISERIQLVSLPKSYRHIHLSDIDVNNASRMEAFSAILDFVEQYPSAEQKGLYLYGD
                     MGIGKSYLLAAMAHELSEKKGVSTTLLHFPSFAIDVKNAISNGSVKEEIDAVKNVPVL
                     ILDDIGAEQATSWVRDEVLQVILQYRMLEELPTFFTSNYSFADLERKWATIKGSDETW
                     QAKRVMERVRYLAREFHLEGANRR"
     gene            24791..26101
                     /gene="der"
                     /locus_tag="ACGEUI_000729"
     CDS             24791..26101
                     /gene="der"
                     /locus_tag="ACGEUI_000729"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFF8165585.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRQFSLIDTGGIDDVDAPFMEQIKHQAQIAMEEADVIVFVVSGKEGVTDADEY
                     VSKILYRTNTPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDAIV
                     ENLPVEEAEENDDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DADGQEFTMIDTAGMRKSGKIYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTIDKDNHTVAKWEADIRDQFQFLTYAPIIFVSALTKQ
                     RLNKLPDLIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVSVKP
                     PTFVVFVNEEELMHFSYLRFLENQIRAAFTFEGTPIHLIARKRK"
     gene            26308..29406
                     /locus_tag="ACGEUI_000730"
     CDS             26308..29406
                     /locus_tag="ACGEUI_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFF8165586.1"
                     /translation="MMARLIPGRVRNEGIKLYEQGLVSFQDDNKGILQIEVETYQVQY
                     GADDEDITCQCDTFHMKHYCKHIAAVEYFLKNDQKGKLFLKQLTNQTKIKETTKKMTS
                     FGSLFLDSLAMNEDDSVKYRLSALGSRSPFSSDYWWSLKINRLPDDRSYVIRDIKGFL
                     QLIKKEGFYQIGKNYFEQLSWLQFDPSSQALIEFLWRLASDTDKGDNENIFPNHARHL
                     RLPSGFFEEGIHYLTSLYDFTFEGPSQTYHHLFVRSLEAEAGLYEFKVEVHRKSIELQ
                     IAEKNVQYLFDNDYLLYQDTFYHLTLKQRKMVQAIRSLPIEADLAKHIHFDLDDHAKL
                     AASLSDFKQIGLVEAPKSFAIRDFEVTFQFDLLNRDEISCQLMFDYGNYQVSDKASLE
                     ALPFASHLKKEEKINRSLLAFGFSPQFYSKKRLTSAKELYTFFEETVPCFERLGNVAL
                     STAIQALQVKEMPKIAIRRNQGLLDISFDFSTIIENDIDQAVTALFQNNPYFVSQTGQ
                     LVVFDDETQKVSKSLQELRARQLKNGHLQLDGIRALQVSKLFEGMTSVHFSKELEELA
                     YHLQHPETFSIKPLPVKAQMRDYQRNGVQWLSMLNHYGFGGILADDMGLGKTLQTLAF
                     LASHLKSDSKVLILSPSSLIYNWFDECQKFTPQLDVVVSYGLKQIRDQIIEEGHQITI
                     TSYSSFRQDFETYQAFHYDYLILDEAQVIKNAQTKISHCLRAFNTANCFALSGTPIEN
                     KMLEIWSIFQIVLPGLLPTKKEFLKLTAEQVSRYIKPFVMRRKKEDVLPELPDLIETN
                     YSNEMTDEQKAIYLAQLRQMQDQIRNSSDVDISRQKIEILSGITRLRQICDTPSLFMD
                     YQGKSGKLDSLRILLTQIKENGHRALIFSQFRGMLDLAKQEMTALGLTSYQMTGSTPA
                     NERQEMTRAFNNGSKDAFLISLKAGGVGINLTGADTVILIDLWWNPAVEMQAISRAYR
                     IGQKENVEVYRLITRGTIEEKILELQESKRNLVTTVLDGNESRASMSIEEIKEILGLN
                     TH"
     gene            29649..30251
                     /locus_tag="ACGEUI_000731"
     CDS             29649..30251
                     /locus_tag="ACGEUI_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165587.1"
                     /translation="MVKNQFPLVADGIAISDPAKQMALYENEDLITNIRGYYQDKEYD
                     DIARNEEFTAKATSRQTPSSKRFCSNDEKHHYVKEARQKAKQDLKEKRQAYLAKEMAY
                     VPKQVSKKQQPADSSPSQKQATTEMSRFTKKLHQDNYILAELPKEYKEPKNLPQQGTT
                     KKNNYDFLKSSQIYNNKEMRQQREKTIAQELNLSRFEDLP"
     gene            30291..31619
                     /gene="murC"
                     /locus_tag="ACGEUI_000732"
     CDS             30291..31619
                     /gene="murC"
                     /locus_tag="ACGEUI_000732"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFF8165588.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFSEDNITPDMELIVGNAFRENNKEVAYALRHQIPFKRYHDFLGDFMKSF
                     ISFAVAGAHGKTSTTGLLSHVLKNITDTSYLIGDGTGRGSANAQYFVFESDEYERHFM
                     PYHPEYSIITNIDFDHPDYFTGIADVRNAFNDYAKQVKKALFVYGEDDELKKIEAPAP
                     IYYYGFEEGNDFIAYDITRTTNGSDFKVKHQGEVIGQFHVPAYGKHNILNATAVIANL
                     FVAGIDMALVADHLKTFSGVKRRFTEKIINDTIIIDDFAHHPTEIVATIDAARQKYPS
                     KEIVAIFQPHTFTRTIALLEDFACALNEADSVYLAQIYGSAREVDKGEVKVEDLAAKI
                     IKPSQVVTVENVSPLLDHDNAVYVFMGAGDIQLYEHSFEELLANLTKNNQ"
     gene            31665..32147
                     /locus_tag="ACGEUI_000733"
     CDS             31665..32147
                     /locus_tag="ACGEUI_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFF8165589.1"
                     /translation="MLIRQVQGSDLEVIATIESDNFSPQEATTRAVLEEHIRLIPDTF
                     LVALIDQEIVGYIEGPVVTTPILEDSLFHGVTKNPKTGGYIAITSLSIAKHFQQQGVG
                     TALLAALKDLVVAQQRTGLILTCHDYLISYYEMNGFINQGISESQHGGTLWYQMIWKL
                     "
     gene            32259..33833
                     /gene="mltG"
                     /locus_tag="ACGEUI_000734"
     CDS             32259..33833
                     /gene="mltG"
                     /locus_tag="ACGEUI_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFF8165590.1"
                     /translation="MALTDFKDKDQQDQQRSFKEQILAELEKANQIRKEKEEELFQKE
                     LEAKEAARRTAQLYAEYKRQDAFQKESIAHNNKTAKHFQAIKGAVMTSEALKPTLLSE
                     KENSSLKTTNKRVVQANELQETASKESQVPLTIEKGHSVRRKLSKRQQTERAAKKIST
                     VLISSIIITLLAVTLAGAGYVYSALNPVDKNSDAFVQVEIPSGSGNKLIGQILQKKGL
                     IKNSTVFSFYTKFKNFTNFQSGYYNLQKSMSLEEIASALQEGGTAEPTKPSLGKILIP
                     EGYTIKQIAKAVEHNSKGKTKKAKTPFNEKDFLDLVTDEAFIQDMVKRYPKLLATIPT
                     KEKAIYRLEGYLFPATYNYYKETTMRELVEDMLAAMDATLVPYYDKIAASGKTVNEVL
                     TLASLVEKEGSTDDDRRQIASVFYNRLNSGMALQSNIAILYAMGKLGEKTTLAEDATI
                     DTTINSPYNIYTNTGLMPGPVASSGVSAIEATLNPASTDYLYFVANVHTGEVYYAKTF
                     EEHSANVEKYVNSQIQ"
     gene            33906..34388
                     /gene="greA"
                     /locus_tag="ACGEUI_000735"
     CDS             33906..34388
                     /gene="greA"
                     /locus_tag="ACGEUI_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFF8165591.1"
                     /translation="MAEKTYPMTLTEKEQLEKELEELKLVRRPEIVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQISTLETKIRYAEIIDSDAVAKDEVAIGKTVIVQEVGTTD
                     KDTYHIVGAAGADIFSGKISNESPIAQALIGKKTGDKVRIESPAATYDVEIISVEKTN
                     "
     gene            complement(35011..35934)
                     /gene="yidC"
                     /locus_tag="ACGEUI_000736"
     CDS             complement(35011..35934)
                     /gene="yidC"
                     /locus_tag="ACGEUI_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFF8165592.1"
                     /translation="MKLTLNRILFSGLALSILLTLTGCVGRDAHGNPKGMIWEFLGKP
                     MSYFIDYFANNAGLGYGLAIIIVTIIVRTLILPLGLYQSWKASYQSEKMAFLKPVFEP
                     INKRIKQANSQEEKMAAQTELMAAQRAHGINPLGGIGCLPLLIQMPFFSAMYFAAQYT
                     KGVSTSTFMGIDLGSRSLVLTAIIAALYFFQSWLSMMAVSEEQREQMKTMMYTMPIMM
                     IFMSFSLPAGVGLYWLVGGFFSIIQQLITTYLLKPRLHKQIKEEYAKNPPKAYQSTSS
                     RKDVTPSQNMEQANLPKKIKSNRNAGKQRKR"
     gene            complement(36016..36294)
                     /locus_tag="ACGEUI_000737"
     CDS             complement(36016..36294)
                     /locus_tag="ACGEUI_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFF8165593.1"
                     /translation="MQKVRLIVSGRVQGVGFRYATHTLALDIGDIYGRVWNNNDGTVE
                     ILAQSKDSDKIATFIQEVRKGPSKWAKVTYVDVTMANFEDFQDFQIGK"
BASE COUNT        11838 a         6042 c         7422 g        11057 t
ORIGIN      
        1 taactatggt tttaaatgct atttcaaaaa aaatatgata aaatagaaag tactaattat
       61 tttggagatg acatgagtat cgatgattat aggccgacct atatggtaga ggctatttat
      121 gatttaagag caaatgattt gttgcgtcat ggtattacgg ctgttttagt ggatttagat
      181 aataccttaa ttgcatggaa taatcctgat ggcacaccag aggttcgcgc ttggcttgat
      241 gaaatgacta tcgcggatat tagcgtggtt gttgtttcaa ataataaaca ttctcgtgtt
      301 gagcgtgccg tttcacgatt tggtgttgac tttattagcc gtgctttgaa gccatttgct
      361 tatggtattg aaaaagccat tgctcgttat ggatttgatc gcaacgaggt tatcatggtt
      421 ggagatcaac taatgactga tatcagagct agtcatagag ctggaataaa atcagtttta
      481 gtcaaaccac ttgttgcttc tgatgcttgg aatactaaaa ttaaccgctg gcgtgaacgc
      541 cgtgtgatgg caaaattaga agagaaatat ggtaaattat catatcaaaa aggaatttaa
      601 tggaagaatt attttgtata ggctgtggta tccagattca aactgaagat aaagaaaaag
      661 ctggttttac gccagctgct gctttgaaaa agggtatgga aacaggagaa ctctattgtc
      721 agcgctgctt tagattgagg cattataatg aaattacaga tgtccatatt accgatgatg
      781 aatttttaag acttcttcac gaagtgggtg atagtgatgc tcttgtggtt aatgtgattg
      841 atatttttga ctttaatggt tctattattc caggtctatc tcgctttatt tctggtaatg
      901 atgtcttact ggttgggaat aaaaaagata ttttgccaaa gtctgttaag gatggtaaag
      961 ttactcaatg gttgacggaa cgtgcacacg aagaaggtct tcgcccatta gacgttatgt
     1021 taacgagcgc tcaaaataaa tatgcgatta aagacttgat tgggcgtatc aatgagttac
     1081 gtaatggtcg agatgtttat gttgtggggg tcactaatgt tggtaaatca accttgatta
     1141 atgctattat ccaagagatt actggtaaca aggatgtcat cacgacgtct cgttttcctg
     1201 ggacaactct tgacaaaatt gaaattcctt tggatgatgg gacgttcatt tttgatacgc
     1261 caggaattat tcacaggcac cagatggctc attatttatc ccctaaagag ctaaaaatag
     1321 tgagtcctaa gaaggaaatc aagcctaaga cgtatcaatt aaatccagaa caaactctct
     1381 ttttaggtgg tctggctcgc tttgacttta tcaacggtga gcgtcaagga tttacagctt
     1441 tttttgacaa tcaactagaa ttacatcgta caaaattagc tggagcagat gctttttatg
     1501 ataagcatgt tggaactttg ttaaccccgc cagataagaa agagttgact gcatttccta
     1561 aattagttcg tcatgaattt acaattgatc agaaaatgga tattgttttt tctggtctag
     1621 gatggattcg tgttaatggt caaaaagact caaaagcgat agttgctgca tgggctcctg
     1681 aaggggttgc tgtcattgtt cgtaaagcga ttatttaaaa ataaaaaaga agttggaaaa
     1741 aaatgcttac aagtaaacaa agagctttct taaaatctga ggctcatagc ttaaaaccga
     1801 ttgtacaaat tggcaaaaat ggattaaatg accacattaa gactagtatt cgtcaagctt
     1861 tggatgctag agaattgata aaggtcactt tgttgcaaaa tactgatgaa gatattcatg
     1921 aagtagcaga gattttagaa gaagaaattg ggtgtgatac ggttttgaaa attggtcgta
     1981 tcttgattct ctataaggtt tctgctaaga aagaaaaccg caagttgtcc cctaaagtta
     2041 aagcaatcta gtccttttat ttgtataggg actggtttta tgagacgagg actattgtcg
     2101 cgagaatttt ggagaaagta tggcattaga attgttgacg ccttttacga aggtggaatt
     2161 agaggaagaa aaaaaagaaa gcaaccgcaa acagattggg attttaggag ggaattttaa
     2221 tcctattcac aatgcacacc ttgtggttgc agatcaagtt cgccagcaat taggattaga
     2281 tcaggtgcta ttgatgcctg aatgtaaacc accgcatgtg gatgccaaag agactattga
     2341 tgagaagcat cgtcttcgca tgttggaatt ggctattgaa gatgttgaag ggctagctat
     2401 tgaaacctgt gaacttgaac gtcagggaat cagctatacc tatgatacga tgctttattt
     2461 gactgagcag catcctgatg ttgattttta ttttatcatc ggagctgata tggttgatta
     2521 tttgccaaag tggcaccgta ttgatgaact ggtcaaactc gttcaatttg ttggagttca
     2581 acgtccaaaa tataaagctg gaacctctta cccggtcatt tgggtagatc ttcctttgat
     2641 agatatttct tctagtatga ttagggattt tattaaaaaa ggtcgtcagc ccaattatct
     2701 cttacctaag cgggttttag actacattac tcaagaaggt ttgtatcaat gacatatgaa
     2761 gattatcttc cttatagcag gaccgaactg ttagcaaaaa ttgcagagca gatgagccct
     2821 aaacggttta aacatgtact tggcgtggaa aaggcagcct tgtccttggc agagtgttat
     2881 ggttgtaacc cagacaaagc agggttagca gctcttttac atgattatgc caaggagtgc
     2941 cctgaccagg tctttttaga cttaattgac aagtaccaat tatctccaga attagctaaa
     3001 tggaataata atgtctggca tggtatggtt ggtatttata agattcaaga agacctaggg
     3061 ctaaaagaca aagatatctt gcgagctatt gaaattcaca ccgttggtgc tgctgagatg
     3121 accttgctgg ataaggtctt atacgttgct gattatattg aagaaggtcg catttttcct
     3181 ttggttgatg acgctagaaa aatcgcgaag ctcgatttga atcaggctgt tgcctatgaa
     3241 acagtgaaca ctgtcgctta tttagcctca aaagcgcagc ctattttccc acaaactctg
     3301 gatacctata atgcgttttg cagctattta aaggaatact aatggataat gatatgagga
     3361 ataaaatgaa aaaagaagaa ctcttaaaaa ttgttgtgga agcaactgag gaaaagcgtg
     3421 ctaaagatat tttagccttg gatttagagg ggttaactag tttgaccgat tattttgtga
     3481 ttgcgagtgc aacaaacagt cgtcaattag aggctattgc ggataatatt cgtgaaaagg
     3541 taaaagaggc tggtggagat gctagccatg ttgaaggtaa tagccaagca ggctgggtct
     3601 tgcttgattt gaccgatgtg gtggtacatc tttttttaga agacgagcgt taccactata
     3661 atcttgaaaa attgtggcat gaagcgcctg ctgtggctct agatgcctat ttagcttaat
     3721 gttacaaaga gaccttgtca atacgacagg gtttttgagg atgataatgg aacataatta
     3781 tgaaaaattt gcgtcagttt atgatgctgt gatggatgat tctctatatg acttgtggac
     3841 agatttttca cttcgacatt taccaaagtc taagggtcga aatcgtttgt tagagttggc
     3901 ctgtggaacc ggtattcagt cagttcgttt tgctcaagca ggttttgatg tgactggttt
     3961 agacttaagt caagacatgc tggctattgc taaaaagcgt gcgcaatctg ctaaaaagaa
     4021 aattgatttt attcaaggta acatgctgga tttgtcacag gtggggcagt ttgattttgt
     4081 aacctgctat tcggattcca tttgctatat gcaagacgaa gttgatgttg gcgatgtttt
     4141 taaagaagtt tatgatgttt tagcaaacga tggcatcttt atttttgatg tgcattcaac
     4201 ctatcaaacg gatgagtgtt tccctggtta ttcctatcat gagaatgcag atgattttgc
     4261 aatggtttgg gatacctatg ctgacgaggc tcctcactct gttgttcacg aactcacttt
     4321 ctttatccaa gaagacgatg gacgtttttc tcgctttgat gaagtgcatg aggaacgaac
     4381 gtatgagtta ttgacttatg atattttgtt ggaacaagca gggttcaagt catttaaggt
     4441 ctatgctgat tttgaagata aggagccaac taagaccagc aagcgttggt tttttgtagc
     4501 atataaatag aagaaaaaag tagccttttt agctatagga tttaatgaat tatcttgaat
     4561 gttacttata tgaaaagagt tcattaggca ttaaatgcta ttatcaaagc ttagaagtat
     4621 tggagacatt acgattgaca ctatgtctga aggttgctac caactttaaa gaaagcattt
     4681 tctattatac gttgtgctta agcatttgat ttaactctta ttagccctat aaaagactat
     4741 ttagtacaat ttgaggagat tctatgactg ttaccggaat tatagctgag tttaatccgt
     4801 ttcacaatgg acacaaatat ttgctagaaa cagcagaggg actaaaaatt attgctatgt
     4861 caggaaattt tatgcaaaga ggagagcctg ctcttattga taaatggatt cgatcagaga
     4921 tggctcttaa aaatggtgca gatattgttg tggaattgcc ctttttcgtt tcagttcaat
     4981 cagcagacta ttttgctcaa ggagctattg atattctttg tcaattagga attcagcaat
     5041 tagcatttgg gacagagaat gtgattgatt atcaaaaact gattaaggtc tatgagaaaa
     5101 aatcagagca aatgactgcc tatttatcga cgcttgaaga tacatttagc tatccacaaa
     5161 aaacacaaaa aatgtgggaa atctttgctg gagttaaatt ttcagggcaa acgccaaatc
     5221 atattttggg gttaagttac gcaaaggcta gcgctggaaa gcatatacaa ctatgtccta
     5281 tcaagcgtca aggggctgcc taccattcga aagacaaaaa tcatctgcta gcttctgcaa
     5341 gtgccattag acagcacctg aatgattggg atttcataag tcattcagta ccaaatgcag
     5401 gtttacttat aaacaatccc catatgtcat gggaccatta tttttcattc ttaaaatatc
     5461 aaattctgaa ccattctgat ttaactagta tttttcaggt caatgatgaa ttggcaagtc
     5521 gtattaaaaa agcgataaaa gttagtcaaa atattgacca cctagttgac actgttgcta
     5581 ccaaacgcta tacaaaagct cgtgtaagac gtattttaac ctacattttg gttaatgcta
     5641 aggaacctac tttacctaaa ggaattcata ttctagggtt tacctcaaaa ggacaagcac
     5701 atttaaaaaa attaaaaaaa agcaggcctc tgattactag aattggagca gaaacttggg
     5761 atgagatgac acaaaaagca gacagcatct atcaacttgg gcatcaggat atcccagaac
     5821 aaagttttgg aagaatacct attataataa aaaatgagag gctcaattaa actctaattg
     5881 agtttttttg ggggcttgac tctaacatta cgttgatggt ttatacttca gttaatccaa
     5941 tgaggaaatt ttataacaat aattaaaaaa gtcagtcaaa agatatactt tcgaagaaca
     6001 ggctaacgtt tttacaagat tatatcagta atcattttga caattgtagt aatggaatgt
     6061 tgccaaaatt tagaacgttg tatgttatgg atgacaagat tttaaaggaa tactaattaa
     6121 acgggaggtc gtgaaccgga agctgttgtc actcaagcgc caaaatctgt ctgtaacata
     6181 atattgatgt atgggaacgc aattatttgg tttttattgt gatgcttaaa acgattttat
     6241 atttctattt taccaaagct tttcaaagtg ctataataag ggagttagag tcaatattag
     6301 taacgttaat gttgacaata ttcaataaaa tactattaaa aggagacaaa aatgggacgt
     6361 aaatgggcaa atattgttgc taaaaaaact gctaaagatg gagcaacatc aaaggtctat
     6421 gccaaatttg gtgttgaaat ttatgtggct gccaaacaag gcgagccaga tccagagtta
     6481 aatacagctc ttaaatttgt cattgatcgt gcaaagcaag cacaagtgcc aaagcatgtt
     6541 attgataaag cgattgataa agctaaagga aacacagatg aaactttcgt agagggacgc
     6601 tatgaaggtt ttggtccaaa tggttcaatg attattgtgg atactttgac atcaaatgtt
     6661 aaccgtacag cggcaaatgt acgtacggct tacggtaaga acggtggcaa tatgggagct
     6721 tcaggatctg tatcctactt atttgataaa aaaggggtca tcgtttttgc tggtgatgat
     6781 gctgacagtg tctttgaaca attacttgaa gcggatgtag acgtagatga tgttgaagca
     6841 gaagagggaa caataacagt ttacactgcc ccaacagatc ttcataaagg tatccaagca
     6901 cttcgcgata atggtgtaga agaattccaa gttactgaac ttgaaatgat tcctcaatca
     6961 gaagtagttt tggaaggtga tgaccttgaa acttttgaaa agcttattga tgcacttgaa
     7021 tcagatgatg atgttcaaaa agtgtatcat aatgtagcag atttttaaat tgacatagaa
     7081 taataaaaag agttgaaatc tatttcagct ctttttaagt gatgctttta taagtaagta
     7141 ccaggtaaca tagtcgttgt tagaagataa tgaacaatag taaatcagta tcaacttgat
     7201 aggaatcaat tattagtgaa ctctaattaa aaatggtaaa ctagacacaa gaacaaaaag
     7261 gagagatagg atgattaaaa aacagtattt aggaagggca acaattgccc tagcatcgac
     7321 ccttgtgcta gcagcttgtg ggtcatcaaa aacagctgag tcaggaaatc aggggagttc
     7381 taaagaagtc ttatttgcaa cggttggaac aacagctccc ttttcttatg aaaaaggtgg
     7441 tcaactaact ggctacgata tcgaggtggc taaagcagtc tttaaaggat cggatgatta
     7501 caaggtgtcc tttaaaaaaa cagaatggtc ttctatcttt acaggtttgg actcaggaaa
     7561 ataccaaatg ggtggtaaca acatttcctt taccaaagag cgttcagcta agtacctttt
     7621 ttcttatcca atcggatcaa caccttctgt tttagttgtt ccaaaagaca gtgatatcaa
     7681 gagctttgat gatatccaag ggcatacgac acaagttgtt caagggacta caagtgttgc
     7741 acagttagaa gactttaata agaaacacag tgacaacccg gttactctaa aattcaccaa
     7801 tgagaacatt actcaaatgt taaccaattt gagtgaaggc aaggcagatt tcaagatttt
     7861 tgacgctcca acggttaacg ccattattaa aaatcaaggg ttagataatc tcaaaacaat
     7921 tgaactcacc tcaacagagc aaccatttat ttattttatc tttagtcaag atcaagagaa
     7981 attgcaaagc tttgttaaca agcgtattaa agagttgact gctgatggaa ctcttagcaa
     8041 gcttgccaaa gagcaccttg gcggtgatta cgtgccatca gataaagagt tgaaacttcc
     8101 tacagctaac taaaccatct attaaccagt tattcatcta gaagactggt ttttctgatc
     8161 ggaaacaata ttatagttta tgaggttatg ttataaaaat tgcaaatagc cttaatcaaa
     8221 atgagatgtt aaagtgcttt ttgagaagtg aatattgtat aagaagtatg ctttgggttt
     8281 tagtcctcaa ataccaagtt ttttgttgac taaacaaata aggatggtta gcatagcata
     8341 tagtttaaaa ctatggtata tgctagccat tatcaatttg aaaactatat ctaattaaga
     8401 tacaataatg atcgtaaaca aaatgaatcg aggataacgc tatgtctttt aaaaaatgga
     8461 ttggaatagt cggaatcgct ttagcatcaa ctgtattggt tgcttgttct gaaaaacaag
     8521 atgataaaaa cactttaact attggggtga tgacaaaaac agaatctgat caagcgagat
     8581 gggataaagt tgaagaatta ttgaaaaaag acaatatcac tttgaagtac aaagaattta
     8641 cagattactc gcaacctaat aaggcagttg ctaatggtga agtcgatatt aatgccttcc
     8701 aacactataa tttcttaaat aactggaata aggaaaataa ggaacatttg gtggccattg
     8761 ctgataccta tatcagccca attaaccttt tctctggaac cagtcaagat ggaaaggcta
     8821 aatacaaatc agtagctgat ctgccaaatg gtactcaaat tgcagtacca aatgatgcga
     8881 ccaatgaaag ccgtgccctc tatgtgctgc aatcagctgg cctcattaaa ttgaatgtgt
     8941 ctggtgatca attagcaacc attgccaata tctcagagaa caaaaagaaa ttagatatca
     9001 aggaattaga tgctagccaa acggcacgtg ctttagtatc tgctgatgca gctgttgtca
     9061 ataatagtta cgctgttcct gcaaaaattg actacaaaac ctctcttttc aaagaaaaag
     9121 cagatgacaa ttcaaaacag tggattaaca ttattgcagg tcaaaaagat tgggaaaaat
     9181 cagagaaggc tgatgctatt aaaaaactca tcaaggccta ccaaactgat gaggtgaaga
     9241 aagtcgtgga aaaaacttca aatggtatag atgtatccgt atggtaaatt ttagccattg
     9301 ttataacaag agtgtggtcg tgtttaaaac acatgctttc ttgcatgctt gaggaagagt
     9361 ttacgactaa tttaccttta gaaagctatt taagaccttt tcccttttag gatttagaaa
     9421 ccttgggaag actattgcat gttatcttaa tagtcatgat tatgcaggcg atgaaaacat
     9481 tgctatcgta gattactgtc gccatatcgt actaatagag gagttattaa aatcttatga
     9541 atgaagcaat aattcaatta gatcatattg atattacctt ccgtcaaaaa aaacgggtta
     9601 tcgaagctgt taaagatgtc acggttcata tcaatcaagg agatatttat ggcattgttg
     9661 ggtattcagg tgccgggaaa tcaactcttg tgcgtgtgat taacctgcta caggcaccaa
     9721 caaacgggaa aatcactgtt gatggggatg tgacttttga ccaggggaaa attcaattgt
     9781 cagctgatgc ccttcgccaa aaacgacgtg atattggtat gatcttccaa catttcaatt
     9841 tgatggccca aaaaacagcc aaagaaaatg tagcatttgc gctccgtcat tcgtcgttaa
     9901 gtaagactga gaaagaacac aaagttatcg aactgctaga acttgtaggt ttatcagagc
     9961 gagcagataa ctaccctgcc cagttatcgg gtgggcaaaa acaaagggtg gctattgcgc
    10021 gtgctctagc taacgatcca aaaattttga tttcagatga ggccacatca gcactagatc
    10081 caaaaaccac taaacaaatt ttggcgctct tgcaagaatt aaaccgcaaa cttggcctta
    10141 ccattgttat gattactcat gaaatgcaaa ttgttaagga tatttgtaat cgtgttgctg
    10201 ttatgcaaaa tggtgttttg attgaagaag gttctgtcct tgatatcttt tcaaatccaa
    10261 aagaagcctt gacacaggaa tttatcacaa cagctacagg gattgatgag gctttagaaa
    10321 aaattaacca acaagacatt gttaaacatt tgcctgcaaa tgcccttcta gctcagttga
    10381 aatatgcagg aacttctacg gatgagcctc tacttaatag catttaccgt cagtttgaag
    10441 tgacagctaa tattttgtat ggcaatattg agattttaga tcatattcca gttggtgaca
    10501 tgattgttgt cttggaaggg caagctgaaa atattcttgc ggctgagaaa gccttgcatg
    10561 aagctggtgt tgatgttagt attttaaaga gaggagccta gatgtcacag ttaattcaaa
    10621 cttatttgcc aaatgtttat gaactcggat ggtctggaga tgctggttgg ggcttagcga
    10681 tttggaatac tttatacatg accattgtgc cttttattgt tgggggtgct attggtctct
    10741 tattaggctt gttattagtt ttaacaggac cggacggtgt tattgaaaac aaaacgattt
    10801 gttgggttat tgataaagtt acctctattt ttagagccat tccttttgtt attttgattg
    10861 ctattttagc tagctttacc tatttgcttt taagaacaac tttaggagca acagcggcct
    10921 tggtaccttt aacctttgcg acttttcctt tttatgcccg tcaggtccaa gttgtttttt
    10981 cagaattaga caagggtgtt attgaagcag cacaagcttc aggtgctact ttctgggata
    11041 ttgtcaaagt gtatctcagt gaaggtttac cagatcttat tcgtgtttct acagtcacct
    11101 taatttcttt agttggggaa actgccatgg caggagctat cggtgctgga ggattaggaa
    11161 atgtagcgat ttcttatggt tataatcgtt ttaacaatga tgtgacttgg gtagcgacta
    11221 ttattattct tttaattatt tttgctatcc aatttattgg tgatagcttg actagaaggt
    11281 ttagtcataa ataaacgaaa caacacccta agtaaaaact tagggtgttg ttgtttagat
    11341 gatgtcatta gcttttcttc gttttactta gtataagccc tattaaaaaa ccaatagcag
    11401 caaagataat ccatcccatt gagaattgcc ctagtggaac agttttttgg aaaaaagtta
    11461 caacggcttc tggtaaatga aataatccag tcatttgtga tagtgttagg agtgcatcaa
    11521 atatggcagg aataaaggtt agaccaattg tagttcgata gacaatagga tcattattga
    11581 agcatttttg tgccaaaaca aggaaaatta aatcgactgt taatgggtat aaaaggaata
    11641 aaacaggagc tgaccagttg ataatgactg ataagccacc aaaataaaag aaagctgata
    11701 gtaaagtaaa gatagttgcc caagcaatat gagataaagc aggcactaat ttatggaagt
    11761 attcagcact tgacgtgatt aagcctgttg aagtggttag acaggccagg aaaatcacaa
    11821 cagctagaaa tgcttgtccg atgccaccta ggtaaaaacg agaagcatga cttaagattt
    11881 gaccgccatt aactggatta ccatgaaggg taaagctgcc atcaataaaa ggaaataatg
    11941 attgtgatgt tgctccaata cgaccgacaa agatatagac aagtgctaat agcaaaatgg
    12001 caatagcccc agaaataagt gttattttgg tcatttcttt gtccgtctta gcgccaaatt
    12061 gtttggtagc ctcaatgact aaaatagcaa aaacaagaga agctagtgca tccatagtgc
    12121 cataaccttg aattaatcct gctacaaaag gaaaatcttt aaaggcatta ttaacaccga
    12181 ccccagcgtt aaaagcatca ccgtaatttc cagcaggatg gacaaaggac gcgataacca
    12241 aaatagaaat aacaactaac aacgttggcg ttaaaaattt tccgatattt tcagctagtt
    12301 tactaggttt gatagcaagg aagtaggaca gaccaaaaaa tagtatagca taaatagctt
    12361 tattggttgt attatctcct aagataggag cgataccgac tgaaaaagaa gtggctcctg
    12421 ttcttggaat agcaaaaaat gggccaatag ataaatacaa aatggaagaa tagaagattg
    12481 cataccattt agagatgggg cgtgccaaac tttcaacatc gcctgatcca gatttagcaa
    12541 cagcaatgac accgagtaaa ggcaaggaga caccagttaa acaaaaacca atgatagacc
    12601 atagaatctg atgacctgag tagatgccta aaaaagctgg gtaaatgagg tttgctgctc
    12661 caaaaaatag cgcaaatagc atgaatccaa tgacgatata tacatttttt tgtttcattt
    12721 ataaatagtc tttctattga tactaatccg aagattctaa caattttaat tatattatgt
    12781 gtcaaaataa caacaaaggt tattatatct aagattactt cgatagtcaa tagaaattga
    12841 ctgaaaattc ttagcacaaa aaatggttaa gatatgtagc tcattcgatt tgacgaatag
    12901 atagaattat attattataa aaaacatgta actttagtaa ggatgtaacg tatgaaaaaa
    12961 atatatgatt tatgggttag ggttagtcta atcaaaaaaa taggtatcgg tgttgtaata
    13021 ggagtcatgt tgggcatttt ggctcctgat cttacaggtt ttagtatttt aggaaagtta
    13081 tttgttggtg gtttaaaggc tatcgcacct ttgcttgtat ttgctttggt ttcccaagct
    13141 atctcccatc agaaaaaagg gaaacagact aatatgacat taatcattgt tctgtattta
    13201 tttggtacct ttgcgtcagc tttagtagca gtactgacag cttatctatt ccctttgacg
    13261 ttagtgttaa atacacctgt aaatacagaa ttatcaccac ctcaaggtgt tgctgaagtt
    13321 tttcagtcac ttctattaaa attagtggat aatccgatca atgctttagc tactgccaat
    13381 tatattggtg tgctatcatg ggcaattatt tttggactag cattaaaggc tgcaagtaaa
    13441 gaaacaaagc atcttataaa aacagcggca gaagttactt cacaaatagt tgtttggatc
    13501 atcaatctgg cacctattgg gattatgagt ctggtgttca ctacgatttc tgaaaacggt
    13561 gttggaatct tatctgatta tgcttttttg atactagttt tagtaggaac aatgcttttt
    13621 gtcgccttag tggttaatcc acttattgcg gttttgatta cacgacaaaa tccttatccc
    13681 cttgtcttga gatgtttacg tgagtcaggt cttacagcat ttttcacacg aagttctgca
    13741 gccaatattc cagtcaatat gcaattatgc caaaaaattg gattgagtaa agatacttat
    13801 tcagtgtcaa ttccacttgg agcaactatt aatatgggtg gggcagcaat taccatcaat
    13861 gtcctaacgc ttgctgctgt acacactttt ggtattccta ttgattttct aacagccctc
    13921 ttacttagcg ttgttgctgc tgtctctgct tgtggtgctt ctggtgttgc tgggggatcg
    13981 ttgttactta ttcctgtagc atgtagtttg tttggcattt caaatgatct tgcgatgcag
    14041 gtggttggtg ttggttttat tgttggtgtt atccaggatt cttgtgagac agccttaaat
    14101 tcgtcaaccg atgttctttt tacagctatt gctgaaaatg ctttttggaa acgtaaaaaa
    14161 gcatagctag atttaacata gtaaaaaaca tccttggaaa acgccaagga tgttttttta
    14221 ttatttaaga tagccaagat aatcaaattt ttcaaaggtg tggtcattag cgatggccac
    14281 aatgatagta ttcggttcta aaggttcaaa aggtttgaca ttagtatcta aggttttaac
    14341 ttctttttga cgcatgccaa tcacatttaa ctcatattta cgacgaacat ctagttcgga
    14401 aagagattgt ccttcccagc ttttaggaat gacaaattca atcatcgaaa taccatgttc
    14461 caaatagatg atgctttcga tatgacgtct taacagattt gaagctacac gttttccaga
    14521 atcacgttca ggggtgatga ctttagttgc tccgatgcca tatagaactt cttcaaaaat
    14581 tttattttta gccttggcaa taatggttgg aacaccgagt tttttacagt gcatgactgc
    14641 aagaacagaa gattccaagt tattccctga agcgataacg acagtatcac aatgttcaat
    14701 ccctacagct aatagaaatt ctttgtcagt aatatctcca acagcggctt tagtgaccaa
    14761 gtcggccacc tctttgacat gactttctct gatatcaata gcaataacgt cttgatcaaa
    14821 gttacttaat tctctggcaa cagtacggcc aaaaatacca aggccaagaa cgccgacagt
    14881 tttacgtttt aacatgtttt ctccttaacc cactagaata tcagtagtgg catattgaat
    14941 ggttttttct tttcgttgaa tcagactgat gaggacagta attgggccaa cgcggccgac
    15001 gaacatcaag acaatgacaa taagacgtcc agcagtagac aattgtggtg tcaaatccat
    15061 agatacccca actgtcgcga tggcagaaat agattcaaat aagagtggta taggagcgat
    15121 atgaggctcc acgctaagta ataaaataaa acctatcata agaactgcaa aaaagaagat
    15181 aagaactgtc atggtctgct taattgtctt atttgcgata attcggttac gaaaggtaac
    15241 ttctgattgc cctgaaagtt ccgctttaaa gagtaagaaa gtaatggcag cagtcgtcac
    15301 tttaatacca ccagctgttc caccaggagc accaccaata accatttgaa tcatataaag
    15361 aatgttagtc ggtgctaggg tatcgttata agagattgtt gcaaagccag ctgttctcat
    15421 agtgactgtt tgaaaaaatg aaaccataag ttgctgatgt aaactaaaat tggcaatggt
    15481 tttgctgtta tctttttcta aaaaccaggt taagaaggtt ccaagaaaaa gaataacagc
    15541 tgtggtttgt agtactagac gagactgatt ggataattta cgaaaagttg ccccataaca
    15601 atgaggtttt tcaaaaaagt attttttaaa agctactcct aaatcaaccc aaaccgcaaa
    15661 accaagtcca ccagagataa tcaagaacgt aatgataaca tttaatgtag gatttaacat
    15721 aaagtctttt aaactagaag atcctaaatt gtcaaaacca gcgttacaaa acgccgacac
    15781 agctaaaaaa atactattga agataccatt tttccacccg aagcggggga tgaagtcaat
    15841 catgataact atcgcagcaa aggcttctag actaaaggtt actttataag caaaaaaaag
    15901 gtagtgcttt aaatctttgc tatcacctcg atttaacgct gattggagca aggtttgatc
    15961 gctcaatcgc atttttcgct tgagggcaaa agtactcaca gctattaacg tcaccaagcc
    16021 aagaccacca atctgcatta gtgccatagc aatggtttgt ccgataccgt tatacacttc
    16081 agcgacggga acaacagaaa gtccagtcac acaaaccatg gaaaccacat taaagaaatg
    16141 atcaagatag acggtattag gaccattttg ataatgggta aagggcatgg ataacaagag
    16201 agtcccaatc agaatcacaa tggcaaaact aaatgttaag cgctgtgtga ctgaaagaga
    16261 tttgataaaa gagcgtttca tgtttcctcc agacataata tctcctacta taataacaaa
    16321 aataagttaa aaaagctaag tttcatctta gcctttggta ctattttata gaggtttttt
    16381 gttaggcaag ccagcttttc ttggatactt gttaggtgtt tcctttttct tctcaacaat
    16441 ggtgatgaag cgagaatctc catttggcaa ttggtagcta tggtttttaa tgactttacc
    16501 aaaaagaaga caaagagcat tcttagcttc ttctaactct tggtcagcag cttgtgcctt
    16561 taaggcgatt aattttcctc caatttttaa aaaggggatg gttaattcag atagaacctg
    16621 catccgagca accgctctgg cagtaacaac atcaaattga cctcgaaagg ccttgtcctg
    16681 tccgaaatct tctgctctac catgaaaaaa gtggacattt tccaagccca attcctgagc
    16741 aaggagtgtc agaaaagaaa tccgcttatt aagagaatca ataatggtaa cctcaaggtt
    16801 tgggaaaaga attttcatgg gtaggctagg aaaaccagct ccagctccaa tgtccaataa
    16861 tttgattggt tcattagcta aaaagccctg taaaatagga gcgatggaat cataaaaatg
    16921 cttgaggtaa acctcatttt cttctgtaat agctgttaaa ttaattttag tgttccattc
    16981 cactagtgat ttgaagtagg tgtcaaattg ttctttttgt ttcgatgata aggaaaaacc
    17041 atcttcctct agtgttcggt aaaagtcttg gggtgtcatt tgtatcctct gtttttgata
    17101 gttgttacca ttctatcata aaatagccta taacaaaatc caataaatca tcagaaataa
    17161 cacctcatta cttgctatta gcctagaaat tgctataatt gagacacaaa gattaaagga
    17221 gacccactat gccaacagtt ttgattatct tagtcgtttt aggagtgctt gcgctatggt
    17281 taatgattag ttacaatagc ctggttaaat ctcggatgca cacaaaagaa gcttggagtc
    17341 agattgatgt gcaattaaag cgtcgtaatg acttgattcc aaatcttatt gaaaccgtaa
    17401 agggatatgc tagctacgag caaaagacat ttgaaaaaat cactgatttg cgtgcgcgtg
    17461 ttgcgaatgc ctcaactcct caagaaacca tggcggcttc taacgaattg agtaaacaag
    17521 tgaccagttt gtttgccgtt gctgaaaatt acccagactt aaaagctaac gaaaacttct
    17581 tgaaattaca agaagagttg accaatacgg aaaataaaat ctcatattct cgtcaactct
    17641 ataattcaac aacgtctaat tacaaccttc aattagaatc tttcccaagc aatatcgctg
    17701 gtaaattatt tggttttaaa ccaagtgaat tcttacaaac accagaagct gaaaaagaag
    17761 ttccaaaagt tgaatttaac ttttaaatat gaaattatca tctagtaggc aatcataaga
    17821 gaaaggagaa ttatgcttta tcaacagatt tcacaaaata agcaacgaac agttgtttta
    17881 ttagttgttt tcttcgctct cttagcactt attggagctt cagcaggcta tctgctgtta
    17941 gataattatg ccatgggctt ggtccttgct cttgtcattg gggtgattta tgctaccagt
    18001 atgatttttc aatccaccag tcttgtaatg agtatgaaca atgctagaga agttacagaa
    18061 aaagaggctc caggcttttt ccatattgtc gaggatatgg ctatggtggc ccagattccg
    18121 atgccgagag tttttattat tgaagatcct tctttaaatg cttttgcgac aggatctagc
    18181 cctcaaaatg ctgctgttgc agcaacgaca ggtttacttg aagtcatgaa tcgtgaagaa
    18241 cttgaaggtg ttatcggcca tgaaattagc cacatccgaa attatgatat ccgtatttcg
    18301 actattgctg tagctttggc gagtgctgtt accgttattt ctagtatcgg tgggcgtatg
    18361 ctatggtatg gtggcggttc tcgcaggcaa agagatgacg gagatgatga tgttttaaga
    18421 atcattacat tacttttgtc gttattatct ttgcttttag cgcccctagt agcaagtttg
    18481 attcagttgg ctatttctcg tcaaagagaa tatctagctg atgctagttc tgttgaacta
    18541 acaagaaatc cccaaggtat gattaaggct cttgaaaaat tgcagttatc tcagccaatg
    18601 aagcatcctg ttgatgatgc tagtgcggcc ttgtatatta atgagcctcg caaaaaaagg
    18661 agcttcagtt cattattcag cacccatcct cctattgagg agaggattga aaggttaaaa
    18721 aacatgtgag cgtttggggt ttttgctcaa gcctcacttt tttatgtctt tgggcagata
    18781 atctaaaatg gcaggaatta tcaaaaatat ctgaacaaaa agaaaaaaat gagtgaaaca
    18841 atggttaaaa atagaatgat ttaaatctta aaattaaaaa acgtcagaaa ataaatgcaa
    18901 aactattgac aaatctttta gatagtagta aaaataaatg agagttttat taggagaaga
    18961 tgatgttagc tatttccgaa atcagaaaac acccagacgg tctctcgttt gatcgtttat
    19021 gtgatgtcaa aagtatgtta cttgagcgtg accaacagat tattgacata aaggcagtta
    19081 aagctgtcgg taatgtttgc tatgataagg ggctttatct gttagattat caattgagtt
    19141 acgaagtgat attaccgtct agccgttcta tggttccggt ctgtttaagt gaagtccaac
    19201 acattcaaga attatttata gaggcgacag atttagctga taagaaagag ttggttgaag
    19261 acaatctggt attagtttta gacaaagacg ctattaacct cgaagaaagt attgtggata
    19321 atatcttact tgctattccg ctacaggtct tgacagagga agaaaagaaa tcaaaagagt
    19381 tgccagcagg tcaaaattgg gcagttttga cagaagaaga ctaccagtgt ctaaaagaag
    19441 aaaagcaaaa agaaaataat ccttttgcta gcttgcaagg gttgtttgat gaataaaact
    19501 gaatattaaa gatgtctgta atttttattt gaaaaaacag ctattctttg atatactctt
    19561 tagagaatat ggttacttat ctaaagtaga aggtattttg accatagagg gcagagaagg
    19621 taatgactat tagatgcttc atattttatt cgctagaaga ctatttgacc attagaactg
    19681 aaatagtcta ggatatgaga tgaatttcat agttttcttt ctgggagaaa aagatagatt
    19741 aagaggataa gggttggtat aaatgacaaa gaaaatttta attattgaag atgaaaagaa
    19801 tctggctaga ttcgtttctc ttgagctgca acatgagggt tatgaagtca ttgttgaggt
    19861 caatggtcgt gaagggttag aaactgcttt ggaaaaagag tttgatttaa tcctgcttga
    19921 cttaatgtta ccagagatgg atggttttga agtgacccgt cgtttgcaaa ccgaaaaaac
    19981 aacgtatatc atgatgatga ctgcgcgtga ttctattatg gatgtggttg caggtttaga
    20041 ccgcggtgca gacgactata ttgttaaacc gtttgccatt gaagaactac ttgcccgtat
    20101 tcgtgctatt ttccgccgtc aagatattga atctgagaag aaagtgccta gtcaaggcat
    20161 ttatcgagat ctagttttaa atccacaaaa ccgttcagtt aatcgtggcg acgatgagat
    20221 ttctctcact aaacgtgaat atgatttgct taatattttg atgactaata tgaatcgtgt
    20281 catgacacgt gaagaattat tgtcaaatgt ttggaaatat gatgaagccg ttgagactaa
    20341 tgttgtagat gtctatattc gttatctccg cggcaaaatt gacattccag gcaaggaatc
    20401 ttatatccaa acagtgcgtg gcatgggata cgttattcgt gagaaataag tcatatggaa
    20461 aatcagaaac aaaaacagaa gaaatataaa aattcgttac caaaacgact atctaatatc
    20521 ttttttgttc tttttttctg cattttctct gcctttacac tgattgccta tagttcaaca
    20581 aactatttct tattgaagaa agaaaagcag tcagtctttc aagctgtaaa tattgttaga
    20641 gttcgtcttt ctgaggtgga ctctaatttt acattagaga acttagcaga agttttgtac
    20701 aaaaacgata aaacacatct gagaattgat gacagaaagg gcagtcgagt cattaggagt
    20761 gagcgcgata tcacaaatac tctagatgcg aatcaagata tttatgtcta taacattgat
    20821 aaacagatga tttttaccac agataacgaa gaatcatctc ctggcttgca tggtcctatc
    20881 ggtcgtgtgt atcacgacca tatcgaagat cagtatcgtg ggttttccat gacacaaaag
    20941 gtatattcta atcggactgg aaaatttgtg ggctatgttc aagtctttca tgatttaggc
    21001 aattattatg tcattagagc aagactgctg ttttggctac tagttgttga gttatttggc
    21061 acaagcttag cctatttaat catcttaatt actacgcggc gctttttgaa gcctttacac
    21121 aatttacatg aagtgatgcg taatatctct gaaaatccta ataatttaaa cctgcgctca
    21181 gatatttcgt caggagatga aatcgaagaa ttgtctgtta tctttgacaa tatgttagac
    21241 aaattggaga cacatactaa gttgcaatca cgttttatta gtgatgtcag ccatgaatta
    21301 cgaacaccgg ttgcgattat taaagggcat attggtctct tacaacgttg gggtaaagat
    21361 gacagtgata ttcttgaaga aagcttgaca gcaacagcgc atgaggctga ccgtatggca
    21421 atcatgatta atgatatgtt agacatggtt cgcgtacaag gctcatttga gggacatcaa
    21481 aatgatatga cagttttgga agattctatt gaaactgttg ttggtaattt tagagtttta
    21541 agagaagatt ttatctttac atggcagtca gaaaatccaa aaacgatagc ccgtatttat
    21601 aaaaatcatt ttgagcaggc tttgatgatt ctgattgaca atgctgtgaa gtattcccgt
    21661 aaagaaaaga aaatcgcgat taacctttca gtgactggca aacaagaagc tattgttaga
    21721 gttcaagata aaggcgaagg aatttctaaa gaagatattg aacatatctt tgaacgcttt
    21781 tatagaacgg ataaatcacg taatcgaaca agtacccagg ctggattagg gattggcttg
    21841 tctattctca agcaaattgt agatgggtat catttacaga tgaaggttga aagtgaatta
    21901 aatgaaggtt cagtgtttat cttacatatt cctttggccc agtctaaaga gagttagagt
    21961 agcttaaggc attgttaatg tgacaggtat agtatgtaat cattagataa tagtagcagt
    22021 cttttgccac aactggttag aagctgatgc tattttttta gcaatgatgg atgacatgaa
    22081 agaaatactg tcaatcgcga tgtcttttgt tatccttgag aatatggtaa aatagtatat
    22141 tagattaatt tgatagaatt ggggtgtttg cgtgcgttgt ccaaaatgta attaccataa
    22201 atcgagtgtt gttgacagta gacaagctga agacggcaac accattcgtc gtcgaagaga
    22261 atgtgaacag tgtcatactc gctttactac ctttgagcgt gttgaagaac tccctttatt
    22321 agtcatcaaa aaagatggca cacgagagca attttctaga gataaaatat taaatggggt
    22381 tgttcaaagt gcgcaaaaac gacctgtctc gagcacagat attgaaaatg tcatttctcg
    22441 tattgaacaa gaagttcgaa caacctacga aaatgaagtg tcaagtactg ctattggtaa
    22501 tttagtgatg gatgagttgg ccgaactcga tgaaatcaca tatgtacgct ttgctagtgt
    22561 ctacaagagt ttcaaggacg ttgatgaaat cgaagaactt ttgcaacaaa ttacgaatcg
    22621 cgtacgcgga aagaaaaaac gtttaaataa tgatgaaacc aattgatact tttacatatc
    22681 taaaacgcaa caaagtaact cttgattcgg tgactttaat acagttgtac tttccaatta
    22741 ttggaagtga cgcggtctct atctaccaat actttattca cttttttgac gatggtttac
    22801 aacgtcataa attcagcgat attttaaatc acctacagtt tggtatgaaa cgttttgaag
    22861 atgctcttgc tatattgaca gcaatggaat tagtgagtgt gtatcagcta tcagatacat
    22921 atcttattac gttgcaccag ccgcttagcc gtgacttgtt ttttcaacat ccagcttatt
    22981 ctcgattatt agaacaaaag attggagagg tagctgtctc tgagttgcag gtaactgtcc
    23041 caagtcaagc aagaaatatt tctaagcgat tttcggatat ttttggggtg caaggagatt
    23101 tgacgaacgt acctcaaaag ccacaaaaga attttgacct ttcaagtttc cagcaattaa
    23161 tggttcgtga tgggttacaa tttgaagata atcaaaaaga tatcattagc ctttacagta
    23221 ttgctgaaca gtatgatatg acgtggtttg atacttatca aatagctaag gcgacggctg
    23281 ttaatgggaa aattcgacct gaacgcttat tggctaaaaa aaatcaaagc atgacaaaac
    23341 caagcaaaga aaatttttca caggctgagc aaattatttt gcgagaagca aagcaagaca
    23401 gtgctttagt ctttttggaa aaaattaaaa aggctagacg tgcaacgatt actaaggatg
    23461 aacgcatttt attgcaaaca ttggccaaaa tgaattttct tgatgatgtt atcaatgtca
    23521 tggtgcttta tacctttaat aagacaaaat ctgctaattt acagaagagt tatgtcttaa
    23581 aaatggccaa tgatttcgcc taccaaaagg tgtcaacggc tgaagaagct atagtggttt
    23641 tgcgagcctt cacggatcgt caaagtcgtc ggcaatcaaa agtaaaaaca agtcaaagta
    23701 atgtgcctaa gtggagcaat cctgattacc aagagacaac aagtcaagaa gagcaggcga
    23761 aactagatca gttcaaacag gctgctttga agaggttaga aaacctagga aaagggggtg
    23821 actagatgga aaagattgga gaaaccatgg cgaaattggg tcaaaacact cgtgttaata
    23881 gcgaccagtt gattcaaacc attttagcgg atcctgaagt agcaagcttc atcagtcaac
    23941 atcacttatc tcaagagcaa attaacctta gcttatctaa gttcaatcaa tttttggtgg
    24001 aacgccagaa gtatcaactc aaagatccct cttatattgc taagggctat cagcctattc
    24061 tagctatgaa tgagggctac gctgatgtgt cttatttgga aaccaaagaa ttggtagaag
    24121 ctcaaaaaca ggcagctatc tctgaacgga ttcaactggt tagtttgcca aaatcctatc
    24181 gccatattca tttatcagat attgatgtta ataatgcgag ccgtatggaa gccttctctg
    24241 ctattttaga ttttgtggaa caatacccat cagcagagca aaaaggcttg tatttgtacg
    24301 gagatatggg aattggcaag tcttacttgt tggctgctat ggcccatgaa ttgtctgaga
    24361 aaaaaggtgt gtcaacgacc cttctacatt ttccgagctt tgccattgat gtaaaaaatg
    24421 ccatttcaaa cggctctgtt aaagaagaaa tcgatgcggt caaaaatgta ccggtgctca
    24481 ttttagatga tattggtgca gagcaagcca cttcttgggt ccgtgatgag gtgttgcagg
    24541 tgattttaca ataccgtatg ttagaggaat tgccaacctt ctttacctct aattacagct
    24601 ttgcggattt ggagcgaaaa tgggcaacca tcaaaggaag tgacgaaact tggcaggcca
    24661 aacgtgtcat ggaacgcgtg cgttatttgg ctagagagtt tcatttagaa ggggctaacc
    24721 gccgttaagt ttgagatagc aatcaagaag aatagctaca cgtatgagtg agtgaaagaa
    24781 aaggagaatc atggttttac ctaccgttgc cattgtgggc cgtccaaatg tgggcaagtc
    24841 caccttattt aatcgaatcg caggagagcg catttcaatt gtagaagacg ttgaaggggt
    24901 tactcgtgac cgtatttatg ctactgggga gtggcttaac cgccaattct cattgattga
    24961 tacaggtggt attgatgacg ttgatgcccc gtttatggag caaatcaagc accaggctca
    25021 aattgccatg gaagaagctg atgttatcgt ttttgttgtt tctggtaaag aaggggtgac
    25081 cgacgctgac gaatatgtgt ctaaaattct gtatcgaacc aataccccag tcattttagc
    25141 tgttaataag gttgataatc ctgaaatgcg taatgacatt tatgattttt attcacttgg
    25201 tttgggtgat ccttatccag tttcttctgt tcatggtatc ggaacagggg atgtcttaga
    25261 tgcgatcgtt gaaaacttgc cagtagaaga ggctgaagaa aatgatgata ttattcgctt
    25321 cagtttaatc ggtcgtccta atgttgggaa atctagcttg attaacgcta ttttaggtga
    25381 ggaccgtgtt atcgctagcc ctgtcgcagg gacaacacgt gatgccattg acacgcattt
    25441 tacggatgct gacggtcaag aatttaccat gattgacacg gctggaatgc gcaaatctgg
    25501 caagatttac gaaaatacag agaaatattc tgtcatgcgt gccatgcgtg ccattgaccg
    25561 ctccgatgtg gtcttgatgg ttatcaatgc tgaagaaggt atccgtgaat acgacaagcg
    25621 tatcgctggc tttgcacacg aagctggtaa aggaatgatt atcgtggtta acaaatggga
    25681 taccattgac aaggataatc ataccgttgc taagtgggaa gccgatattc gtgaccaatt
    25741 ccaatttttg acatacgctc cgattatttt tgtgtcagct ttgacgaagc agcgcctcaa
    25801 caaattgcct gacttgatta aacgtatcag tgagagtcag aataaacgta tcccatctgc
    25861 tgtcctcaat gatgttatca tggatgctat tgccattaat ccaacgccaa cggataaggg
    25921 taaacgtttg aaaattttct atgccactca agtgtcagta aagccaccaa catttgtcgt
    25981 ctttgttaac gaggaagaat tgatgcactt ctcttatcta cgcttcttgg aaaaccagat
    26041 tcgcgcagcc tttacctttg aagggacacc gattcatcta attgctcgca aacgaaaata
    26101 agaaatgaac atcaactaaa atgctgacct aagaaataat ctcatcattg ataaggactg
    26161 tgttgtgtat gttacaggag acagtccttt ttataatacc tttaagggtg tagacggaag
    26221 attagtagca aacaaagaaa aaggattttg acaagaaata tcagtcggtt tttgctataa
    26281 tgaaaggata agcaaaaagg tggaaaaatg atggctagat taattccagg acgagtaagg
    26341 aatgaaggta ttaagcttta tgagcaaggt ttagtttcat tccaagatga taataaaggc
    26401 attcttcaaa tagaagtgga gacttatcag gttcaatatg gagcagatga tgaggatatc
    26461 acttgtcaat gcgacacatt tcatatgaaa cattattgca agcatattgc agcagtcgag
    26521 tattttttaa aaaatgatca aaaaggtaag ctttttttaa aacagttaac aaatcaaaca
    26581 aaaatcaaag aaacaacaaa gaaaatgaca tcttttggta gcctcttttt agatagctta
    26641 gccatgaatg aagatgattc tgttaaatac aggctgtctg ctcttggtag tcgaagccct
    26701 ttttcgtcag attactggtg gagtctgaaa atcaatcgtt taccagatga tcgttcttat
    26761 gtgattcggg atattaaagg ctttttgcag cttattaaaa aagaaggctt ttatcagatt
    26821 ggaaaaaatt attttgaaca actttcatgg ctgcagtttg acccatctag tcaggccctt
    26881 attgagtttt tatggcgctt agcatctgat actgacaaag gggacaacga gaacattttt
    26941 ccaaatcatg caaggcattt gcgcttacct agtggttttt ttgaagaagg tattcactat
    27001 ttgacgtcac tttatgactt tacttttgag gggccttctc aaacttatca tcatcttttt
    27061 gtccgttctt tggaagcaga ggcaggcctt tatgagttta aagtggaagt gcatcgcaaa
    27121 tctattgaat tgcaaattgc tgaaaaaaat gtgcaatacc tctttgataa tgactatctt
    27181 ctttatcaag atacctttta tcacttgacg ttaaagcaac gaaaaatggt tcaggctatt
    27241 cgtagtctgc ccattgaggc cgacttggct aaacatattc attttgattt ggatgatcat
    27301 gcgaaattag ctgcaagctt atcagatttt aaacagatcg gtctagtaga ggctcctaaa
    27361 agctttgcta ttagagactt tgaggtgact tttcaatttg atttgttaaa tcgagatgaa
    27421 atcagttgtc agcttatgtt tgattatggc aattaccaag ttagtgacaa agcaagttta
    27481 gaagcacttc cttttgctag tcatttaaaa aaagaagaaa aaatcaatcg ttctttactt
    27541 gcttttggct tctcgccaca attttactca aaaaagcgac taacaagtgc aaaagagctc
    27601 tatacttttt ttgaagaaac agttccatgt tttgagcgct taggtaatgt tgcgttgagt
    27661 acagctatcc aagcattaca agtgaaagaa atgcccaaga tcgctattag acgcaatcag
    27721 ggattactag atatttcgtt tgatttttca accattattg aaaatgatat tgaccaagct
    27781 gttacagctc tttttcaaaa taatccttat tttgtgagtc aaacgggtca attagtggtt
    27841 tttgatgatg aaactcaaaa agttagcaaa agtttacaag aattaagagc aagacagcta
    27901 aaaaatggtc acttgcaact ggatggcatt agagctctac aagtatccaa actttttgaa
    27961 ggcatgacat ctgttcattt ttcaaaggaa ttagaagagt tagcctatca tttgcagcat
    28021 cctgaaactt tttcaataaa accgttacca gttaaggctc aaatgcgaga ttatcaacgc
    28081 aacggtgttc agtggttatc aatgctcaat cattatggct ttggtggcat tttagcagat
    28141 gacatgggac ttggaaaaac tctccagaca cttgcttttt tagctagtca cctgaaatca
    28201 gatagtaaag tgctcatttt atctccatct agtcttatct ataattggtt tgatgaatgt
    28261 caaaaattca cacctcagct agatgttgtg gtttcttacg gacttaaaca aatcagagat
    28321 caaatcatcg aagaaggtca tcaaataacc ataaccagct attcctcctt tagacaggat
    28381 tttgagacct atcaagcgtt tcactacgat tatcttatct tagatgaagc tcaagtgata
    28441 aaaaatgcac aaactaagat ttcacactgt ttacgtgcgt ttaatacagc caattgtttt
    28501 gcgctttcgg gcactcctat tgaaaataaa atgcttgaaa tttggtccat tttccaaatt
    28561 gttttgccag ggttgctacc gactaaaaaa gagtttttaa aattaacagc tgagcaagta
    28621 tcacgctata tcaaaccctt tgtgatgcgt cgtaaaaaag aagatgtcct accagaatta
    28681 cctgatttaa ttgaaactaa ttattcaaac gagatgacag atgaacaaaa agctatttac
    28741 ttagcgcagt taaggcagat gcaagatcaa attcgaaatt ctagtgatgt ggatattagc
    28801 cgtcaaaaaa ttgagatttt atctggtatc actcgattga ggcaaatctg tgacactcca
    28861 agtctcttta tggattacca aggcaaaagt ggtaaactag atagtttgcg aatcttattg
    28921 acacaaatta aagaaaatgg tcatcgggct ttgatttttt ctcaatttag agggatgctg
    28981 gacttggcca agcaagagat gactgctcta gggttgacct cttatcaaat gacgggatct
    29041 acccctgcca atgaacgtca agaaatgact agagccttta ataacggttc caaggatgct
    29101 tttttgattt ccctcaaggc aggtggagtt ggaatcaacc ttacgggagc agacacagtg
    29161 attttaatcg atttatggtg gaatcctgct gttgaaatgc aggcaatcag tcgcgcctac
    29221 cgtattggtc aaaaagaaaa cgttgaagta tatcgcttaa tcactcgagg gacgattgaa
    29281 gaaaagattt tagagcttca agaaagtaag cgcaatttgg tgactacagt tttggatggc
    29341 aatgaaagcc gagcaagtat gtccattgaa gaaataaaag aaattttggg gctaaacact
    29401 cattaatctt ccttgatttt agtgacatta aaaacccttt aaagaagatg acaccaaaag
    29461 caccttatat aacctcgtta aaacgcttct ttttgaaata tctttattga tatagcgatt
    29521 gtcatttgct agtaatgatt aaaacattta gaaaaagaca cgtcatagaa tcatcaagga
    29581 atgctttgaa aaataacagt atttaagtta taatagagga tgctacaaga agggagaatc
    29641 aaaaaaggat ggtaaaaaat cagtttcctt tagttgcaga tggcattgct attagtgatc
    29701 cagcaaagca aatggctctg tatgaaaatg aagatctcat taccaacatc cgtggctatt
    29761 accaggacaa ggagtatgat gatattgcta gaaatgagga gtttactgct aaagcaactt
    29821 ctcggcagac cccttctagt aagcgctttt gtagcaatga tgaaaaacac cattatgtca
    29881 aagaggcgcg tcaaaaagca aagcaagatc tcaaggaaaa aagacaggct tatcttgcca
    29941 aagaaatggc ctatgttcct aaacaggttt ctaaaaagca acagcctgcg gattcttctc
    30001 cttctcaaaa gcaagcaaca acagaaatga gtcgttttac aaaaaagctt catcaagaca
    30061 actatatttt agctgagttg ccaaaagaat ataaagagcc aaaaaatctt cctcaacaag
    30121 gaactacaaa aaaaaataat tacgattttt taaaatccag tcaaatttac aataataaag
    30181 aaatgagaca acaacgagaa aaaacaattg cgcaagagct taatcttagt aggtttgagg
    30241 atcttcctta ataaatctac tcagcgtatg cagatgacaa aggagttaag atgtcaaaaa
    30301 cgtatcattt tatcggaatt aaagggtcag gaatgagtgc cttggccctt atgttgcatc
    30361 aaatggggca taaggtgcaa ggtagtgatg ttgaaaaata ctatttcact caaagaggat
    30421 tggaacaagc gggcatcact atcttacctt ttagcgagga caacattacg ccagatatgg
    30481 aacttatcgt tgggaatgcc tttcgtgaaa acaacaaaga agtggcctat gctttgagac
    30541 accagattcc tttcaaacgt tatcatgact ttttaggtga ttttatgaaa tcatttatca
    30601 gttttgcagt agcaggtgct catggtaaaa cttctacaac gggtttattg tcacatgtct
    30661 tgaaaaatat cacagacaca tcttatttaa ttggtgatgg tactgggcgt ggatctgcta
    30721 atgcacagta ttttgttttt gaatcagacg aatacgaacg ccattttatg ccttatcacc
    30781 cagaatactc tatcattact aatattgatt ttgaccatcc tgattatttc acaggaattg
    30841 ctgatgtgcg aaacgctttc aacgattatg ctaagcaggt caagaaagct ttgtttgttt
    30901 atggtgaaga tgacgaacta aaaaagatcg aagcacctgc acctatttac tattatggtt
    30961 ttgaagaggg caatgatttt atcgcttacg acattacgcg tactacgaac ggatcggact
    31021 ttaaggtaaa acaccaagga gaggtcattg gtcagtttca cgtacctgct tatggaaaac
    31081 ataacatttt aaacgctaca gcagtaattg caaacttgtt tgtagccggc attgatatgg
    31141 ctctagtggc agaccatctt aaaacttttt caggtgtgaa acgtcgtttt acagagaaaa
    31201 ttatcaatga cactattatt attgatgact ttgctcatca tccaaccgaa attgtggcaa
    31261 caattgatgc ggctagacaa aaatacccaa gtaaagaaat tgtagcaata ttccagccac
    31321 atacttttac tcgcactatt gccttattag aggattttgc ttgtgcttta aatgaagctg
    31381 acagtgttta tttagcccag atttatggtt ctgcacgtga agtagataaa ggtgaagtta
    31441 aggttgaaga tttggctgct aagattatca aaccatcaca agtagtaacg gtggaaaatg
    31501 tctctcctct tcttgatcac gataatgctg tttatgtttt tatgggagct ggagatattc
    31561 agctatatga acattctttt gaggaattac ttgcaaattt aactaaaaat aaccaataac
    31621 atgtcctgcc ttaggcaggt tttctcattc taaaggagtt tgatatgttg attagacagg
    31681 ttcaaggatc cgatttggaa gttattgcta ccattgaaag tgacaatttt tcaccacaag
    31741 aagcaacgac aagagcagtt cttgaagagc acatacggct gataccagat acttttttag
    31801 tggcacttat cgatcaggaa attgttggct atatcgaagg tccagttgtc actactccta
    31861 ttttggagga ttctttgttc cacggggtga ctaaaaatcc taaaactgga ggctatattg
    31921 ctattactag tttgtccata gctaaacatt ttcagcaaca aggggttggg acagctttgt
    31981 tggcggcact aaaagattta gtagtagcgc agcaaagaac aggtcttatt ttgacttgtc
    32041 atgattactt gatttcctat tatgaaatga atgggtttat taaccaagga atttcagagt
    32101 ctcaacatgg tggaaccctt tggtaccaaa tgatttggaa actttagaat cacagctgat
    32161 tcaactgttg gtttgttaaa aataagtaac aaatgcatca tttaattgca attaggatac
    32221 ttttaagtta taatggcggt tgattatatt taggaggatt ggctttgacc gattttaagg
    32281 ataaagacca acaagatcag cagcgaagct ttaaagagca gattcttgct gagttggaaa
    32341 aagcaaatca aatcagaaaa gaaaaagagg aagaactttt tcaaaaagag ttggaagcta
    32401 aagaagcagc taggagaacg gcccagctat atgctgaata taaaagacaa gatgcttttc
    32461 aaaaagagtc tatagcacat aacaataaga cagctaaaca ctttcaagct ataaaaggtg
    32521 cggtaatgac ttcagaagcg cttaaaccga ctttactttc tgaaaaagaa aactcatctt
    32581 taaaaacgac aaataagaga gtcgtgcagg caaatgagct tcaagagact gcctctaaag
    32641 aatctcaagt accgttaact attgagaaag gtcattcagt gagacgaaaa ttaagcaaac
    32701 gccaacagac tgagcgagct gctaaaaaga tttcaaccgt tttgattagt tctattatta
    32761 taaccctttt ggctgttact ctagcaggag caggctatgt ttatagtgct ttaaatcctg
    32821 ttgataaaaa tagtgatgcc tttgttcaag ttgagattcc atctgggtca ggcaataaat
    32881 tgattggtca aattcttcaa aaaaaaggtt taatcaagaa tagcactgtt tttagttttt
    32941 atacaaaatt taaaaacttt acaaattttc agagcgggta ttataatctg caaaaaagta
    33001 tgagtctaga agaaattgct agtgctttac aagaaggtgg tacagcagaa cctaccaagc
    33061 catctcttgg gaagatcttg attccagaag gatacacgat taaacaaata gctaaagctg
    33121 ttgagcataa tagcaaggga aagaccaaaa aagctaaaac accttttaac gagaaggatt
    33181 ttttggattt agtcacggat gaggctttta ttcaagatat ggtaaaaaga tatccaaaat
    33241 tattagcaac tatcccaact aaagaaaaag ctatttaccg tttggaaggt taccttttcc
    33301 cagcaaccta taactattac aaagaaacta ccatgagaga acttgtagag gacatgctgg
    33361 cagctatgga tgctactttg gtaccctatt atgataaaat tgctgctagt ggtaagacag
    33421 tcaacgaggt attgaccttg gcctctttgg ttgaaaaaga aggttcaaca gacgatgaca
    33481 gacgtcaaat tgcaagtgtc ttttataacc gccttaatag cggaatggca ctacaatcta
    33541 atatagctat tttgtatgcg atggggaaac ttggtgagaa aacaaccttg gctgaggatg
    33601 ctactattga caccaccatt aattctcctt ataatattta taccaataca ggtctgatgc
    33661 ctggtccagt tgctagctcg ggggtttctg caattgaagc aaccctaaat ccagcctcaa
    33721 cggattattt atactttgtg gccaatgtcc atactggtga agtttactat gcaaaaacat
    33781 ttgaagaaca ctctgcaaat gttgaaaaat atgtgaatag tcaaattcag taaacacaaa
    33841 catggtgctt aagcaccatg tttgtgtttc tgattcgttc aaaaaagaaa agagagaaaa
    33901 caattatggc agaaaaaacg tatccgatga ccctaacgga aaaagaacaa cttgaaaaag
    33961 aacttgaaga attaaaactt gtgcgtcgtc cagaaatagt ggagcgtatt aaaattgcac
    34021 gttcgtatgg agatctttca gagaactctg aatacgatgc cgcaaaagat gagcaagctt
    34081 ttgttgaagg tcaaatttca acgcttgaaa ctaagattcg ctatgctgaa atcattgaca
    34141 gtgatgctgt tgcaaaagat gaagtcgcta tcggaaaaac agttatcgtt caagaagttg
    34201 gaacaacaga taaagatact tatcatattg taggtgctgc tggagcagat atcttctccg
    34261 gaaaaatttc aaatgaaagc ccgattgctc aagctttgat tggtaaaaaa acaggtgaca
    34321 aggttagaat tgaatcacca gcagctactt atgatgttga aatcatcagc gttgaaaaga
    34381 caaattaatc cgatgaagcc attatttgcc taaccactaa gtgttttggt tgctaatggc
    34441 ttttctagta tcttcaaatg aggttgactg ttaggaaggt caagtattaa tcgcattgat
    34501 tatgagtcaa gaggactata gatcaaagat cgttttcgat ggttatttaa taccaaaaga
    34561 aaagaccagc accttaatgt actgacccca aaaagttgga cactatattt taagtatagg
    34621 aagtgtataa tctaaggaaa ctgacactaa tgattgaccg ttttgcaaga ctttattaat
    34681 gatttcttgc tttattttaa atgaataagt ctgatatgtt tccttcctta acactatcga
    34741 ctccataatg acccatcagt caaacggtat atctcaaacg actacggttt actccaaatt
    34801 cattggctag ttgaggccaa gaaacacctg attttttaat gctaaatttc tagcttatct
    34861 tcatagctta atttcataca aaaaaacccc aatcgttaga tttttttgat atgtcccctt
    34921 tcactagatg agtccagtaa aagggggcat atcgtttttt gtctaacttt tggagtgcag
    34981 ttcataattg tgctaggttt tttgtctttc ttaacgcttt cgctgttttc cagcattacg
    35041 attggatttt atttttttag gcaagttagc ctgttccatg ttttgtgaag gcgtaacgtc
    35101 cttacgagaa gaagttgatt ggtatgcttt aggcgggttt ttagcgtatt cttctttaat
    35161 ttgcttatgc aatcgaggtt ttaagaggta ggttgtgatt aactgttgaa taatactaaa
    35221 gaaaccacca actagccagt aaagaccaac accagcaggg agtgagaagg acatgaagat
    35281 catcatgata ggcatggtgt acatcatcgt cttcatttgc tcacgttgtt cttctgaaac
    35341 ggccatcatt gataaccatg attggaagaa gtaaagagca gcgataattg ctgttaacac
    35401 taaactacgg ctaccaagat caatacccat aaaggtgctt gttgatactc ctttagtgta
    35461 ttgagcggca aaatacatgg cagaaaagaa tggcatctgg atgagaagag gtagacatcc
    35521 aatacctcca agagggttaa tcccatgagc acgctgagca gccattaatt ctgtttgggc
    35581 agccattttt tcttcctgac tatttgcctg tttgatacgt ttgttgattg gttcaaaaac
    35641 aggtttcaag aaggccattt tctcagattg ataacttgct ttccaagatt gatatagacc
    35701 tagtggtaag ataagtgttc taacaataat ggttacgatg atgatagcca gtccataacc
    35761 tagtccagca ttgtttgcaa agtaatcgat aaagtatgac atgggttttc caagaaattc
    35821 ccaaatcata cctttgggat ttccgtgagc gtctcttccc acacagcctg tcaaggtgag
    35881 tagaattgac agtgctagtc ctgaaaagag gatgcgattt aatgttagtt tcaatgtgtt
    35941 tatcctttaa ttttaatata cttatctatt ttacagtttt tttcagaaat tcacaatagt
    36001 tctgaaggcc gatttttact tgcctatttg aaagtcctga aagtcttcaa aattagccat
    36061 ggtgacatcc acataagtga cttttgccca tttggatggt cctttacgaa cttcttgaat
    36121 aaatgtggct attttatctg aatcttttga ttgggccaga atttcaacgg tcccatcgtt
    36181 gttattccag acacgaccat aaatatctcc tatatctaga gctaaagtat gggtagcgta
    36241 tcgaaaacca acaccttgta cacgtccaga aacaattaaa cgtacttttt gcataagaag
    36301 ggcctccttg cttttattat agcacatact tagcaggaaa tctctttagc taattgtct
//
LOCUS       DAXWIQ010000015        33963 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000015 DAXWIQ010000000
VERSION     DAXWIQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33963
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.015"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(56..1090)
                     /locus_tag="ACGEUI_000738"
     CDS             complement(56..1090)
                     /locus_tag="ACGEUI_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HFF8165594.1"
                     /translation="MCYNMVKKDEEKAMKQAILDRYQALKCYQNAGLSNQAFRAIAKE
                     PIIDNRLGSPTFWVIWPIEKENQSAKQLLTFLLDLVEMPFELSGQLHETQTLLTRFHP
                     SLLPDHMFWKELASLVDQAFPGKTLSQAGELEKRLHQFRYVISSQQAQSIRNHYKMIE
                     MTDAQALALFLRSKKGPCLWRQAPDYTLMDSARLHNKLRFEDNKVIFPSQEVSYNIKV
                     LLWFHTEFTLDSTGFFLNEVDAEVVTEKGIVNGASFNYGTDGPRHWDLDVDPISHHDP
                     QFRRDTLKGFRSPKRVFRQWFRAQKDDFMFSYFNAKGLFAYHNKSSFARVKKSAKQFK
                     RQIHPIKGWF"
     gene            complement(1146..2393)
                     /locus_tag="ACGEUI_000739"
     CDS             complement(1146..2393)
                     /locus_tag="ACGEUI_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFF8165595.1"
                     /translation="MLTKLEYWAKQCPNKKAIVADQISLTYQELWQAVLIKDQTIKDS
                     VPYIISHSRYLNQLLSFLRGLKEGSCPIILHPNISGTFQQQIKHVDGELLKKADFAVL
                     SSGTTGKAKLFWRRLSTWTRLFDYQNKVFGMTGNSCLFLHGSFSFTGNLNLALAQLWA
                     GGCLVLSQKLSLKTWLSLWQAKKVSHLYLLPTYLNRLLPYLTKNNMTATHLLTSSQMI
                     SQELLRHYYKKFPQLEIVIFYGASELSFITWCNGRAAVKINGLVGQPFPDVSISFKDK
                     EIFVETPYSVEGMSQPYSVSDLGKMSPAGLILEGRQDDWVNQRGVKCHLPSLVELAHQ
                     APNVKEAHALKIGKGENETLILVLVLTKKDCLAPIKDFLALYLNSGQLPKYYLVIDCL
                     PLKDNGKINREVLLNKIPKQWLS"
     gene            complement(2350..3498)
                     /locus_tag="ACGEUI_000740"
     CDS             complement(2350..3498)
                     /locus_tag="ACGEUI_000740"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HFF8165596.1"
                     /translation="MTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVP
                     IDQVICGNTVGTGGNIGRLMTLYSHLGESVSALTVDMQCASAGAALSVGYAKIKAGMA
                     SNLLVGGIESSSLQPESVYASADWRQGAYKVAQFSPDSISPFAMIEGAERVAREHGFT
                     KEYLNHWTLRSHQKASYCQEQALLADLILDLSGASDQGIRPRLSSKVLSKVPPILGEG
                     HVISAANACLTHDAAAFLQLSSQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHG
                     LGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAYGHPYGASAAIIILHLM
                     RALEIKNGRYGIAAIAAAGGQGFAVLLKYHKEFSHADKARILGKAMPK"
     gene            complement(3893..4042)
                     /locus_tag="ACGEUI_000741"
     CDS             complement(3893..4042)
                     /locus_tag="ACGEUI_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase"
                     /protein_id="HFF8165597.1"
                     /translation="MQLLDNVYGTLRSFAFMRPAKSSNDTSLADALQAGTDMAFILVR
                     GINYQ"
     gene            complement(4243..5028)
                     /locus_tag="ACGEUI_000742"
     CDS             complement(4243..5028)
                     /locus_tag="ACGEUI_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8165598.1"
                     /translation="MAKLRCMHAIFIKQYLKQIMEYKVDFVVGVLGVFLTQGLNLLFL
                     SVLFQHIPSLEGWTFEQIAFIYGFCLIPKGIDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGVILLVTTAGSIVWTLPKVLLFILVIPFATLIYTS
                     LKIATASISFWTKQSGAVIYIFYMFNDFSKYPMSIYHSFLRWLISFIIPFAFTAYYPA
                     SYFLTGQHLLFNIGGLVVVSLLVLALSLKLWKWGLDAYESADS"
     gene            complement(5030..5848)
                     /locus_tag="ACGEUI_000743"
     CDS             complement(5030..5848)
                     /locus_tag="ACGEUI_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8165599.1"
                     /translation="MWSFWKRYRPFLSAGIQELITYRVNFFLYRIGDVMGAFVAYYLW
                     KAVFDSSKQSLINGFTLSDMTFYIIMSFVTTLLTKSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFMEIGFRWIVLMSVGFPFLMVLSGIKVMAGLSILQVLASSCLYLVSLLLA
                     FLINFYFNICFGSSAFVFKNLWGSNLLKNALVAFMSGSLIPLAFFPKMVSIVLSFLPF
                     SSLVYTPVMIVIGKYSLSQIMVALSLQIFWLLVMVVLSQVIWKKVQYHLTIQGG"
     gene            complement(5850..6842)
                     /locus_tag="ACGEUI_000744"
     CDS             complement(5850..6842)
                     /locus_tag="ACGEUI_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8165600.1"
                     /translation="MVMIEVSHLQKNFSKTIKEPGLKGALKSFVHPQREIFEAVKDLS
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGYCRINGKIPQDNRQYYVRDIGAV
                     FGQRTQLWWDLALQETYVVLKEIYDVPEKAFRKRMDFLNEVLDLNEFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIIMIDKGQEIFDGTVTQLKQSFGKMKSLSFELKPGQEQVVSQFMGLPDIT
                     VERHEFSLDIQYDSSRYQTADIIQKTMADFAVRDVKMTDVDIEDIVRRFYRKEL"
     gene            complement(6983..8926)
                     /gene="thrS"
                     /locus_tag="ACGEUI_000745"
     CDS             complement(6983..8926)
                     /gene="thrS"
                     /locus_tag="ACGEUI_000745"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFF8165601.1"
                     /translation="MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQL
                     IDTTRAIEEDGSIEIVTPDHKDAYEVLRHSAAHLFAQAAKRLFPNLHLGVGPAIAEGF
                     YYDTDNAEGQISNEDLPRIEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELIN
                     EHAGAGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGT
                     AWFDKKDLKAYLTRLEEAKERDHRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLE
                     RYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNC
                     PHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQ
                     IQEEFQRALQLIIDVYADFNLTDYRFRLSYRDPNDTHKYYDNDEMWENAQSMLKAALD
                     EMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPHQVTVIPISNEAHIDYAWEVAKT
                     LRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLIVGDKEMEDKSVNVRRYGSKTTHTE
                     SVEEFVENILADIARKSRPDAQA"
     gene            complement(9348..10682)
                     /locus_tag="ACGEUI_000746"
     CDS             complement(9348..10682)
                     /locus_tag="ACGEUI_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFF8165602.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKEELEKEGHEVYIFTTTDRD
                     VKRFEDPTIIRLPSVPFVSFTDRRVVYRGLISSYKIAKHYNLDIIHTQTEFSLGLLGK
                     MIGKALRIPVVHTYHTQYEDYVSYIANGKIIRPSMVKPLLRGYLKDLDGVICPSRIVL
                     NLLEGYEVTIPKRVIPTGIPLEKYIRDDITAEEVTNLKAELGIAGDETMLLSLSRISY
                     EKNIQAIINQMPAILAENAKIKLIIVGNGPYLQDLKHLAMQLEVDKHVTFTGMVPHDK
                     VALYYKACDFFISASTSETQGLTYIESLASGTPIIAHGNPYLDDVVTDKMFGTLYYAE
                     TDLTDAIIDAILKTPVMDKRLLAKKRYEISAQHFGKSIYTFYLDTLIARNSKEAQKLS
                     LYLNHSGKSSSLKLVQGAIHLPKRAAKVTAITSVKVVKAPIKLVHAIKDFLD"
     gene            complement(10684..11682)
                     /locus_tag="ACGEUI_000747"
     CDS             complement(10684..11682)
                     /locus_tag="ACGEUI_000747"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFF8165603.1"
                     /translation="MKVLLYLEAENYLRKSGIGRAIKHQAKALSLVGQHFTTNPRETY
                     DLVHLNTYGLKSWLLMIKAQKAGKKVIMHGHSTEEDFRNSFIFSNLLSPWFKKYLCHF
                     YNKADAIITPTLYSKSLIESYGVKSPIFAVSNGIDLEQYGADPKKEAAFRRYFDIKEG
                     EKVVMGAGLFFLRKGIDDFVKVAQAMPDVRFIWFGETNKWVIPAQVRQMVNGNHPKNL
                     IFPGYIKGDVYEGAMTGADAFFFPSREETEGIVVLEALASRQHLVLRDIPVYYGWVDQ
                     SSAELATDIPGFIEALKKVFSGASNKVEAGYKVAQSRRLETVGHALVDVYKKVMEL"
     gene            complement(11813..12814)
                     /gene="ccpA"
                     /locus_tag="ACGEUI_000748"
     CDS             complement(11813..12814)
                     /gene="ccpA"
                     /locus_tag="ACGEUI_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFF8165604.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILAKGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPVVLAGTVDLDHQLPS
                     VNIDYRAAVSNVVDILAENHKCIAFVSGPLIDDINGKVRLAGYKEGLKHNKLDFKEGL
                     VFEANYSYKEGFELAQRVINSGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPVVQYTRPNLSSISQPVYDLGAVSMRMLTKIMNKEELEEKEILLNHGIKKRGTTK"
     gene            12988..14073
                     /locus_tag="ACGEUI_000749"
     CDS             12988..14073
                     /locus_tag="ACGEUI_000749"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFF8165605.1"
                     /translation="MTKLDQIRLYLDQKGAELAIFSDPVTINYLTGFFCDPHERQLFL
                     FVYHDLAPVLFVPALEVARASQAISFPVFGYVDSENPWEKIKAVLPNTAAKTIYAEFD
                     HLNVNKFHGLQTIFSGQFNNLTPYVQGMRLVKSADEINKMMIAGQFADKAVQVGFDNI
                     SLDATETDVIAQIEFEMKKQGIHKMSFDTMVLTGNNAANPHGIPGTNNIENNALLLFD
                     LGVETLGYTSDMTRTVAVGQPDQFKIDIYNLCLEAQLAAIDFIKPGVTAAQVDAAARQ
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMAGNDLVLEEGMCFSVEPGIYIPGKVGVR
                     IEDCGHVTKNGFEVFTHTPKELLYFEG"
     gene            14122..14787
                     /locus_tag="ACGEUI_000750"
     CDS             14122..14787
                     /locus_tag="ACGEUI_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFF8165606.1"
                     /translation="MDQTIHHQIQQALHFRTAVRVYKEEKISDEDLALILDAAWLSPS
                     SIGLEGWRFVVLDNKPIKEEIKPFAWGAQYQLETASHFILLIAEKHARYDSPAIKNSL
                     LRRGIKEGDGLNSRLKLYESFQKEDMDMADNPRALFDWTAKQTYIALGNMMMTAALLG
                     IDTCPIEGFHYDKVNHILAKHNVIDLEKEGIASMLSLGYRLRDPKHAQVRKPKEEVIS
                     VVK"
     gene            14798..15175
                     /locus_tag="ACGEUI_000751"
     CDS             14798..15175
                     /locus_tag="ACGEUI_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8165607.1"
                     /translation="MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYL
                     ALEGESYELELTYNYGHGDYDLGNGYGHIALGSEHFEADHKKHRQAGFPVTDIKELAD
                     KSARYYFIQDPDGYKIEVIDLNN"
     gene            15320..16246
                     /locus_tag="ACGEUI_000752"
     CDS             15320..16246
                     /locus_tag="ACGEUI_000752"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8165608.1"
                     /translation="MTLLSIIVPCFNEEANILPYFEEMHQLETSMTNQLAFEYIFIDD
                     GSKDNTLGILRELAARFPNVHYLSFSRHFGKEAGLLAGLKEAKGNYITVMDVDLQDPP
                     ELLPIMYAKLKEGYDIVGTRRQNRQGEPLIRSMCSNLFYGLIKHLSDTEMVNGVRDYR
                     LMTRQVVDSILELGEVNRFSKGIFSWVGYRITYLSFENQKRKYGKSRWHFWELLRYSL
                     DGFINFSEMPLTIATWTGTFSFLISIFAILFIIIRKILFGDPVSGWASTVSIILFMGG
                     IQLFCMGIIGKYISKIFLETKKRPLYIIKEKH"
     gene            16457..17140
                     /locus_tag="ACGEUI_000753"
     CDS             16457..17140
                     /locus_tag="ACGEUI_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFF8165609.1"
                     /translation="MEWIKLIGIVIIVLGFILKCDAIATVVVAGLVTALVSGISFIDF
                     LDILGKEFTNQRLLTIFFITLPLIGLSETYGLKHRATQLIQRVQALTVGRLLTLYLII
                     RELAGLFSIRLGGHPQFVRPLIQPMGEAAAKANIGEELTDAEKDDIKAMAAANENFGN
                     FFAQNTFVGAGGVLLIAGTLEQLGYDGNQAKIAFSSILIAIISIIIVAIYNYLFEKKM
                     ERQHQKGDN"
     gene            17140..18066
                     /locus_tag="ACGEUI_000754"
     CDS             17140..18066
                     /locus_tag="ACGEUI_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFF8165610.1"
                     /translation="MANIANLVLETIYILIGLQLFHTAYCTFKDKTNPVYFGTALFWG
                     LLGVTFVGGAFLPNKVIGFIVIVLALLTLFKQVRIGTLPAFNEQKAEESAHRIGNWIF
                     LPVMLMAMISLLLALILPDFSKSAIGIAGILATIAILIITKQKPSALLAENNRMNQQV
                     STSGILPQLLGALGAIFAAAGVGDVIASLIREIVPADSRFFGVLAYVLGMVIFTMIMG
                     NAFAAFTVITTGIGVPFVFALGADPIIAGALAMTAGFCGTLLTPMAANFNALPVALME
                     IKDRNAVIKKQAPIALVLIISHIALMYLLAYY"
     gene            18116..18763
                     /gene="pcp"
                     /locus_tag="ACGEUI_000755"
     CDS             18116..18763
                     /gene="pcp"
                     /locus_tag="ACGEUI_000755"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFF8165611.1"
                     /translation="MKILVTGFDPFGGEAINPALEAIKKLPATIHGAEIKCIEVPTVF
                     QKSADVLQQHIESFQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RADGKAAYFSTLPIKAMVAAIHQAGLPASVSNTAGTFVCNHLMYQALYLVDKYCPNAK
                     AGFMHIPFMMEQVVDKPNTAAMNLDDITRGIEAAIFAIVDFKDRSDLKRVGGATH"
     gene            complement(18879..19598)
                     /locus_tag="ACGEUI_000756"
     CDS             complement(18879..19598)
                     /locus_tag="ACGEUI_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HFF8165612.1"
                     /translation="MSIMITKGQVRLLRTYSYDSNLYTQGLEQLNNNHILLSAGRYGF
                     SKVGVYDLTQEIFSEKIAFPDTVFAEGLTVVEDYFWLLTYKEGVAYKFDKATCNCLGA
                     YPFEGDGWGLAYDKENQCLWMTSGNAFLQKRDPKDFALLDTVLVAIESVPISMLNELE
                     YVDGYLYANIWQTNTIVKLQPDSGKVVATYDISPLLKALNLDKSHYPDLNVLNGIAHL
                     DQQRFLITGKLYPLMLEVVLD"
     gene            complement(19604..20071)
                     /gene="smpB"
                     /locus_tag="ACGEUI_000757"
     CDS             complement(19604..20071)
                     /gene="smpB"
                     /locus_tag="ACGEUI_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFF8165613.1"
                     /translation="MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARI
                     QLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPERTRKLLLKKREITHLANELKGSG
                     MTLVPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR"
     gene            complement(20074..22407)
                     /gene="rnr"
                     /locus_tag="ACGEUI_000758"
     CDS             complement(20074..22407)
                     /gene="rnr"
                     /locus_tag="ACGEUI_000758"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFF8165614.1"
                     /translation="MMKERIMTYLKEHGKSNVNDLAAALEMAGAKHFPSLIKTISKME
                     SQSLLRFSDDGSLALRKEREKKKEPTVQGVFRANKAGFGFLHVDENEDDMFIGRNDVG
                     YAIDGDTVEVVVKKPADRLKGTAAEAKVVAIVDRSLKTAVGTFILDDDKPKYAGYIRS
                     KNQKIQQKIYIKKEPVVLKGTEIIKVDIDKYPIRGHDYFVASVRDIVGHQGDVGIDVL
                     EVLESMDIVSEFPAEVLAEANAISEAPTAKDLIGRVDLRQETTITIDGADAKDLDDAI
                     HIKLLDNGNYELGVHIADVSYYVTEGSALDKEAIARGTSVYVTDRVVPMLPERLSNGI
                     CSLNPNIDRLTQSALMEINSQGHVVNYQICQSVIKTTYRMTYSTVNDMIAGDEEALQE
                     FASIADDVTLMVALHRILEAMRSKRGALNFDTQEAKIIVNDKGMPVDVVLRQRGIAER
                     MIESFMLAANECVAEHFAKAKLPFIYRIHEEPKAEKLQQFIDYASTFGIHIQGTANKI
                     SQEALQAFMAKVEGQPGAEVLNMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRR
                     YPDLLVHRMVREYNQPSQEKRDHFAQIIPELATSSSQLERRAIDAERVVEAMKKAEYM
                     AEYVGEEFDGIVSSVVKFGFFVELPNTIEGLVHITSLPEYYHFNERTLSLQGEKSGKV
                     FKVGQPIRVKLVKADKETGDIDFEYLPSDFDVVEKIKMSDKASRRDRRKSSKSSKGTK
                     KKEPKEVAKAKTKGKTKKGSKKPFYKEQAKKKSRKRS"
     gene            complement(22501..22737)
                     /gene="secG"
                     /locus_tag="ACGEUI_000759"
     CDS             complement(22501..22737)
                     /gene="secG"
                     /locus_tag="ACGEUI_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFF8165615.1"
                     /translation="MYNLLLTILLVLSVLLVIAIFMQPQKNPSSNVFDSSGSEALFER
                     TKARGFEAFMQRFTAVLVFFWLAIALVLAILSSK"
     gene            complement(22783..22929)
                     /gene="rpmG"
                     /locus_tag="ACGEUI_000760"
     CDS             complement(22783..22929)
                     /gene="rpmG"
                     /locus_tag="ACGEUI_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8165616.1"
                     /translation="MRVKINLECSECGSNNYLTSKNKSSHPEKIKVPKYCPKERKVTL
                     HVET"
     gene            complement(22926..24119)
                     /locus_tag="ACGEUI_000761"
     CDS             complement(22926..24119)
                     /locus_tag="ACGEUI_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFF8165617.1"
                     /translation="MNWRQNLKVAWLGNFFTGASFSLVMPFMALYVENLGTPTELVEY
                     YAGLAVAVTALASALFAPVWGKLADRYGRKPMMLRASFVMTFTMGGLAIIPNVFWLLI
                     LRLLTGVFAGYVPNATALIASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLG
                     IRQVFLLVGVILFLCSLMTAVYVKEEFKPVRRFEMIPTKVILKQVKSPQIMLGLFVTS
                     MIIQISAQSVAPILSLYIRHLGQTHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGN
                     HRLLLAALCYSFIMYFSSALAQTSFQLGVLRFAYGFGVGALMPSINSLLTKLTPKEGI
                     SRVFAYNQMFSNLGQVIGPFIGSNVAVVLGYRSVFYVTSLIVFVNLIWSLIIFRKYIK
                     VKDIV"
     gene            complement(24241..25743)
                     /locus_tag="ACGEUI_000762"
     CDS             complement(24241..25743)
                     /locus_tag="ACGEUI_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HFF8165618.1"
                     /translation="MTTMTIAKGNQPIALQLGMLNRHGLIAGATGTGKTVTLKVLAEQ
                     LSLAGVPVFLADIKGDLSNLTKAGEVTDKLAARLATIGVSDYQPQAFPVRMWDVFGQN
                     GQPLRTTISELGPMMLSRLLNLNDTQTGVLNIVFKIADEKGWLLIDLKDLQAILKEVG
                     DHASDYSSHYGNIAKQSIGAIQRSLLTLEQEGAHQFFGEPALDVADLMQLDVASGYGA
                     INILSATKLFQSPTLYTTFLLWLLSELYKLLPEVGDLDKPKMVFFFDEAHLLFKDAPK
                     VFLEKVEQIVRLIRSKGVGIFFVTQNPLDLPETVLAQLGNRIQHAFRAYTPKEQKAVR
                     VAADTFRQNPDLDVARVITELEVGEALISVLNDKGQPSIVERAYIMPPKSSFAVLSEI
                     ESQQLVQSSPFASKYSQSIDRESAYEKLAAKVLEDNRLAQEAIATAQREKEAKEAIKA
                     QAATKKANRRSVGRSHKTVVEKATDAFISTTVRTIGRELVRGLLGSLRKR"
     gene            complement(25933..26526)
                     /gene="coaE"
                     /locus_tag="ACGEUI_000763"
     CDS             complement(25933..26526)
                     /gene="coaE"
                     /locus_tag="ACGEUI_000763"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017469.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFF8165619.1"
                     /translation="MIIGITGGIASGKSTVVKVIRKAGYQVIDADQVVHDLQEKGGRL
                     YEALREAFGNQILKADGELDRTKLSEMLFSNPDNMATSSAIQNQIIKEELAAKRDHLA
                     QSQAIFFMDIPLLMELGYQDWFDAIWLVYVDAQTQLQRLMARNRLDKGKARQRIASQL
                     PIEEKKPYASLVIDNSGDIAALIKQVQSALLLLANPR"
     gene            complement(26523..27350)
                     /gene="mutM"
                     /locus_tag="ACGEUI_000764"
     CDS             complement(26523..27350)
                     /gene="mutM"
                     /locus_tag="ACGEUI_000764"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFF8165620.1"
                     /translation="MPELPEVETVRRGLETLVLGQEIVAVTLKVPKMVKTDLETFALT
                     LPGQIIQSVGRRGKYLLIDLGQLVLVSHLRMEGKYLLFPDEVPDNKHFHVFFELKNGS
                     TLVYQDVRKFGTFDLIAKSQLSAFFAKRKLGPEPKKETFKLKTFEAALLSSQKPIKPH
                     LLDQTLVAGLGNIYVDEVLWAAKVHPETASSRLNKAEIKRLHDETIRILALGIEKGGS
                     TVRTYRNALGADGTMQDYLQVYGQTGKPCPRCGQAIVKLKVGGRGTHICPKCQKKRL"
     gene            complement(27522..28388)
                     /gene="rgg2"
                     /locus_tag="ACGEUI_000765"
     CDS             complement(27522..28388)
                     /gene="rgg2"
                     /locus_tag="ACGEUI_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HFF8165621.1"
                     /translation="MEKELGKTLRRLRKGKQVSISFLADEYLSKSQISRFERGESEIT
                     CSRLLNLLDKLNITIDEFVSAHSKTHTHFFTLLSQARKCYAEKNVVKLTKLLKDYAHK
                     DYERTMIKAILFSIDSSIAPSQEELTRLTDYLFKVEQWGYYEIILLGNCSRFMNYNTL
                     FLLTKEMVASFAYSEQNKTNKMLVTQLSINCLIISIDHSCFEHSRYLINKIDLLLRDE
                     LNFYEKTVFLYVHGYYKLKQEEMSGEEDMRQALQIFKYLGEDSLYYSYKEHYRQIVLG
                     GKGDEDWSEADL"
     gene            28477..28545
                     /gene="shp2"
                     /locus_tag="ACGEUI_000766"
     CDS             28477..28545
                     /gene="shp2"
                     /locus_tag="ACGEUI_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HFF8165622.1"
                     /translation="MKKVNKALLFTLIMDILIIVGG"
     gene            28695..28937
                     /locus_tag="ACGEUI_000767"
     CDS             28695..28937
                     /locus_tag="ACGEUI_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165623.1"
                     /translation="MKQQGHTVMMCLDKTLTIKLSAPLTSNDLVPLSTNEVIIMEPLL
                     HLLPKVVPVLIHLVDKANVSWLTVILQLPILLKTIW"
     gene            29459..29728
                     /gene="stcA"
                     /locus_tag="ACGEUI_000768"
     CDS             29459..29728
                     /gene="stcA"
                     /locus_tag="ACGEUI_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system protein StcA"
                     /protein_id="HFF8165624.1"
                     /translation="MTKKQFYLILAISILCFILAVSPKPPILKHTIERKRTAMTIGHS
                     KQNPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQS"
     gene            complement(30076..30474)
                     /locus_tag="ACGEUI_000769"
     CDS             complement(30076..30474)
                     /locus_tag="ACGEUI_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFF8165625.1"
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNNPFLLPKKVLSLE
                     DDAAILNHLEEDRDVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTG
                     ITYGDDTLADLTHHLLHSDGVIPSLNDKSA"
     gene            complement(30567..31650)
                     /locus_tag="ACGEUI_000770"
                     /pseudo
     CDS             complement(30567..31650)
                     /locus_tag="ACGEUI_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32055..32249)
                     /locus_tag="ACGEUI_000771"
     CDS             complement(32055..32249)
                     /locus_tag="ACGEUI_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFF8165626.1"
                     /translation="MEVLKNGLAIINGGGKDGANIFLTGMAGAAQGVTVCAQTGVFIP
                     WQGYILCGAAGAATNIIWPH"
     gene            complement(32543..32743)
                     /locus_tag="ACGEUI_000772"
     CDS             complement(32543..32743)
                     /locus_tag="ACGEUI_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HFF8165627.1"
                     /translation="MTTMKELTINDMSSISGGNAPGDAVIGGLGGLASGLKFCKLPHP
                     VLTGGCVVGFTVGGAYLGYTAN"
     gene            complement(32756..32972)
                     /locus_tag="ACGEUI_000773"
                     /pseudo
     CDS             complement(32756..32972)
                     /locus_tag="ACGEUI_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016754.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
     gene            33290..33963
                     /locus_tag="ACGEUI_000774"
                     /pseudo
     CDS             33290..33963
                     /locus_tag="ACGEUI_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        10283 a         7287 c         6069 g        10324 t
ORIGIN      
        1 accaaaaatt cagcctaatt gacaatgacg actaccgtta caaatagagt actttctaaa
       61 accaaccctt tataggatga atttgacgtt tgaattgctt agctgatttt ttgactctgg
      121 caaaactact cttgttgtgg taagcaaaaa gtcctttggc attaaaataa gagaacataa
      181 agtcatcttt ttgagctcta aaccactggc gaaatactct ttttggcgat cgaaatcctt
      241 taagggtgtc tcttctaaac tgaggatcgt gatggctaat cggatcaaca tctaaatccc
      301 aatgtctagg accatctgtg ccatagttaa aagatgcacc attgacaatt cctttttcgg
      361 tcactacttc tgcatcaact tcattaagaa aaaagcctgt gctatctaga gtaaattcgg
      421 tatgaaacca aagaagtact tttatattat aggaaacttc ttgacttgga aaaatgactt
      481 tattatcttc aaatcttagt ttattatgta acctggcaga atccatcaag gtataatctg
      541 gtgcttgccg ccacaaacaa ggcccttttt tagacctcaa aaaaagggcc agagcttggg
      601 cgtctgtcat ctcaatcatt ttataatggt ttctgataga ttgagcttgt tgactagaga
      661 tgacataacg aaattgatgc aaacgctttt caagctcacc tgcttggctt agtgttttgc
      721 caggaaaagc ttgatcaact aagctagcca actctttcca aaacatatga tctggcaata
      781 gagatggatg gaatcgggtg agtagcgttt gagtttcatg caactgacca cttagctcaa
      841 atggcatctc taccaaatca agaagaaacg ttagtaactg cttagctgat tggttttctt
      901 tttcaatagg ccatattacc cagaaagttg gagaaccaag tcgattatca atgataggct
      961 ctttagctat agcacgaaat gcttggtttg ataaacctgc gttttgatag catttcaagg
     1021 cctgatagcg atctaagata gcttgtttca tggccttctc ctcatctttt ttgaccatat
     1081 tataacacat ctaaagctga atcgtcactt gtatctcact ataagcaaaa cgaaacctga
     1141 agtggttaac taagccactg tttgggtatt ttatttaaaa gaacttctcg attaatttta
     1201 ccattatctt ttaaaggcaa acaatcaatc acaagatagt acttgggaag ttgtccagag
     1261 tttaaataaa gagctaaaaa gtctttaata ggtgctaggc aatccttctt agtcaaaact
     1321 aatactaata ttagggtttc gttttctcct tttcctatct ttaaagcgtg cgcttctttg
     1381 acgtttggcg cttggtgagc gagttcaact aggctaggca ggtggcattt cactcctcgt
     1441 tgattaaccc agtcatcttg tcgcccttct aaaattaaac ccgctgggct catttttcct
     1501 aagtctgaaa cactgtatgg ttgtgacatt ccttctacac tataaggagt ctctacaaaa
     1561 atttccttat ctttgaaact gatagacaca tcaggaaagg gctgtcctac caaaccatta
     1621 attttgacag cagctctgcc attacaccac gttataaaag ataattcact agccccgtag
     1681 aaaatgacaa tttcaagctg aggaaatttt ttgtaataat gtcttagtaa ttcttgtgat
     1741 atcatttgcg aagaggtcaa taagtgagtc gcagtcatgt tgttcttagt caaataaggt
     1801 agcaagcgat ttaggtaggt cggtaaaagg taaaggtgac tcaccttttt tgcttgccat
     1861 aagcttaacc acgttttcaa agacagcttt tggcttaaaa ccagacatcc cccggcccat
     1921 agttgagcta atgctaagtt aagattgcca gtaaaactaa aactaccatg taagaataga
     1981 caactattac cagtcatacc aaacacctta ttttgataat caaaaagtct cgtccaagtt
     2041 gatagacgac gccaaaataa ttttgctttg ccagttgttc ccgaactcaa aacagcaaaa
     2101 tcagcttttt taagaagctc accatcgacg tgttttattt gctgctgaaa tgtacctgag
     2161 atattagggt gtaaaatgat aggacaactg ccttctttta atcctcgtaa aaaagagagt
     2221 aactgattaa gatagcgact atgactgatg atatatggta cactgtcttt tattgtctga
     2281 tcctttatta aaaccgcttg ccatagctct tgataagtca agcttatctg atcggctacg
     2341 atagcttttt tatttgggca ttgctttgcc caatattcta gctttgtcag catgactaaa
     2401 ctccttatga tattttagta aaacagcaaa cccttggcca ccagctgctg ctatagcagc
     2461 tattccatag cgtccatttt taatctctaa agccctcatt aagtgcaata ttataatggc
     2521 tgctgaggca ccatagggat gaccataagc taatgcacca ccaaatatat tatagcggtc
     2581 caataaatct ggataatggg tttcaaatag accatcaata acggcaaagg cttcgttcca
     2641 ttcaatcgct gtcatatctg ccatccctag accatgtttt tccaaaagga cttgactagc
     2701 cttaatcacc attaaagggc tacgctgtgg gtctcctgct acttctacca catcaatcag
     2761 tttaaaagct gaaggctgac tacttagctg taaaaaggca gcagcatcat gggttaggca
     2821 ggcattggct gcactaatca cgtgaccttc tcctaagata gggggtacct ttgacaacac
     2881 cttacttgac aagcgcggtc gaataccttg atcactggct cctgacaggt ctagaataag
     2941 atcagctaac agggcttgct cctggcaata actagccttt tgatgacttc tcaatgtcca
     3001 gtgattcaaa tactcctttg taaagccatg ctctcttgcc actcgttctg ccccttcaat
     3061 catcgcaaaa gggctgatac tatctggaga aaactgagct accttgtagg ctccttggcg
     3121 ccaatcagct gaagcatata ccgattcagg ttgtaaggag ctgctttcaa tccctcccac
     3181 taaaaggttg cttgccatac ctgccttgat tttagcataa cccacggaaa gagctgcacc
     3241 agctgatgca cactgcatat caaccgtcaa agcagagaca gattctccta aatgagaata
     3301 cagggtcatc aagcgcccaa tattaccacc cgttccgacg gtattgccac aaatcacttg
     3361 gtcaatggga actggatact tattttgtaa agcgttgatg agctttgctc caaggatttc
     3421 tggttgttct ttagcaaatt gtttccctac taaaccgata ggggttctaa ggcctgcagc
     3481 aatataaaca tccgtcatta tattgacctc ctttattttc gatgtcctta aatggttatt
     3541 gtataattat ttctgatggt tttctttttt gaactaataa ctgctaaaaa tacacctaaa
     3601 ttaatttaga gaatctcttt agtctcgcta gtgtgctatc aataaaatct ataaaattct
     3661 ctaacgtcat tgaaatgtca agtcccacaa ctattgtcgt agaagtcact tactttgttg
     3721 ctttaacatt caaactgtaa cctttgtttt tgatacaggg tgactttatt atactggaaa
     3781 accattccta agaaaagaaa atcttatata gaataaagaa ttagacacca taaaaaagct
     3841 aagctgtgac acttaacttt tttatggtat tgacaaacct tgtctttact atttattgat
     3901 agttaatgcc tctaactaaa ataaaagcca tgtctgtacc agcctgcaag gcatctgcta
     3961 agctggtatc gttactagat ttggcaggtc tcataaaggc aaaagatctc aaagttccat
     4021 aaacgttatc tagcaattgc atgcaaccac ttgaaacata tctgcttatg ttattttacc
     4081 gcccttaatt tagaccaatc tgctgatgat ggttccaacc aatgcctacg cttggtaaac
     4141 catcacaaaa aaaggcagcc gtttttacaa cagctgcctt ccttcattat ctaggttttc
     4201 atattctgac ctaaacgatt ttcaatttgt ttaagagtct ctctaggaat ctgcactttc
     4261 ataagcatcc aggccccatt tccaaagctt aagcgacaag gccaaaacca gtaacgacac
     4321 cactaccaag cctccaatat taaagagcag gtgttgacct gttaaaaaat aagaagcagg
     4381 gtaataagct gtaaaagcaa agggaatgat aaaactgatt aaccaccgca aaaaggaatg
     4441 gtaaatggac atagggtact tagaaaagtc attgaacatg taaaaaatat agataactgc
     4501 cccagactgc ttggtccaaa aagaaatgct ggcggtcgca atttttaaag aggtgtaaat
     4561 caaggtcgca aaaggaataa ccaaaataaa caaaagcacc ttaggaagag tccatacgat
     4621 actaccagcc gtggtcacta ataagataac cccaaccaat aattcaccca gggcatccac
     4681 ttgaaaggtc tcaactaaaa catgaaacaa aggactaatt ggtctggtga ggtacttgtc
     4741 aaattctcct ttgcggacaa gccgttgccc taaagcccaa agattgtcaa aaaagaggtg
     4801 atcaattccc tttggaatca aacaaaagcc ataaataaag gcaatttgtt caaacgtcca
     4861 gccttctaaa gaagggatat gttggaaaag taccgataaa aagagcagat taagtccctg
     4921 agtgagaaaa acacctagca ctcccaccac aaaatcaacc ttgtattcca taatttgttt
     4981 gaggtattgt ttgataaaaa tagcgtgcat acatctcaat tttgccatct caccctcctt
     5041 gaatcgttaa atggtactga acctttttcc agatgacttg cgataagact accatcacca
     5101 gtaaccaaaa gatttgcaaa cttagggcca ccattatctg actaaggctg tatttcccaa
     5161 tgacaatcat aactggcgtg taaaccaaag aggaaaacgg tagaaaacta agcactatag
     5221 aaaccatttt aggaaaaaaa gccaatggaa tcaaactgcc tgacataaag gcaaccaagg
     5281 catttttcaa taaattagac ccccaaaggt ttttaaagac aaatgctgaa gagccaaaac
     5341 aaatgttgaa ataaaaattg attaaaaaag ctaataaaag gctaactaaa tacagacaac
     5401 tgctggctag gacttgcaag atagacagcc ccgccatgac tttaatacca gataagacca
     5461 ttaaaaaagg aaagccgaca ctcattagca caatccagcg aaaaccaatt tccataaaga
     5521 ggtaagaggc tgcaaaatga actggtcgaa gcaggcgcat gatgatagaa ccatctttca
     5581 cttcttcgcc aatcataaag gagctgtctg atttggtcag taaagtggtc acaaaactca
     5641 tgataatgta aaaggtcatg tcagacagag taaaaccatt gattaaggat tgcttagacg
     5701 aatcaaaaac agccttccag aggtaataag ccacaaaagc ccccatgaca tctccaatac
     5761 gatacaaaaa gaaattaacg cgataggtta ttaattcctg aattcctgca ctgagaaagg
     5821 gtctataacg tttccaaaat gaccacatct taaagctcct ttcggtagaa acgacgaaca
     5881 atatcctcaa tatctacatc agtcatcttc acatccctga ccgcaaaatc agccatggtt
     5941 ttttgaataa tgtccgcagt ttgatagcgc gaactgtcat attgaatgtc taaactaaac
     6001 tcatgccgtt ccactgtgat gtcaggtaag cccataaact gtgagaccac ctgctcctgc
     6061 cctggcttca actcaaaaga cagactcttc atttttccaa agctttgctt taattgggtc
     6121 accgttccat caaagatttc ttgcccttta tcaatcatga taatccgatc acagagctgt
     6181 tcaatatcac tcaaatcatg ggtcgtcagt aaaatagttg tctcttcttc ttgattaatt
     6241 tgagtaatgg cacgccgaat gttgtccttc acagaaacat caagtccaat cgttggttca
     6301 tctaaaaaca atactttagg attatgcaac aaggaggcag caatatcagc tcgcatccgc
     6361 tgaccgagtg ataaggtccg gacaggatcc ttaataaact cattcaggtc caaaacctca
     6421 tttaaaaaat ccatgcgctt gcgaaaggct ttttcaggta catcgtaaat ttcttttaaa
     6481 acgacatagg tctcttgcaa ggccaaatcc caccatagtt gtgttcgttg accaaaaaca
     6541 gctccaatgt ctctcacata atattgacga ttatcttgtg gaatctttcc gttaatgcga
     6601 cagtaacctg atgtaggctt taaaatacct gttaacatct taatcgttgt tgattttcct
     6661 gcaccattgg cgccaataaa acctaaaatt tgccctttgg gtacctcaaa agataagtcc
     6721 ttaaccgcct caaagatttc tctttgggga tgaacaaacg acttcaaggc tccctttaat
     6781 cctggctctt taatggtttt ggaaaaattt ttttgaaggt gggacacttc tatcatgacc
     6841 atttaccatt cctcgtttct gttatcgtct tatcaccgtt actaaacaat ttattataac
     6901 accataaaaa agacggtgca accgtttgct taatttttta agaaagaaaa agaagcccat
     6961 aaggacctct ttttgccatt gattaagctt gtgcatctgg acgtgacttg cgagcaatgt
     7021 ctgccaagat gttttccaca aattcttcca cagattcggt gtgagtcgtc ttgctaccat
     7081 agcggcgaac attgactgat ttgtcttcca tttctttgtc cccaacaatt aattggtaag
     7141 gaattttgct ggtttgagac gcacgaatct tgtactgcat tttttcattg cggtcatcga
     7201 catctgcacg aacaccacgg tcacgaaggg tttttgctac ttcccaagca tagtcaatgt
     7261 gggcttcatt tgaaattgga atcacggtta cttggtgagg tgccaaccaa gttgggaaag
     7321 cacccttgta agtttcaata aggatagcag tgaaacgttc catagttgag ataacaccac
     7381 ggtgaatcat gactgggcgg tgttcttcgc catcagcacc aatgtatttg agatcaaagc
     7441 gttctggcaa caagaagtcc aactgaatgg ttgatagggt ttcttcgtta ccaagagctg
     7501 ttttcacctg aatatcaagc ttaggcccat aaaaagctgc ttcaccttct gcttcaaagt
     7561 aatctacacc catctcatca agtgcagctt taagcatact ttgagcattt tcccacatct
     7621 catcattgtc ataatattta tgagtatcgt tagggtctcg gtatgacaaa cgaaaacgat
     7681 aatcagtcaa gttgaagtca gcataaacat caatgattaa ttgaagggca cgttgaaatt
     7741 cttcctggat ttgctctggt gttacgaaaa tgtgaccatc attcaaggtc atttcacgta
     7801 cccgttgaag tcctgatagg gcacctgatt tttcgtaacg gtgcatcatg ccaagctcgg
     7861 caatacgaat aggcaattca cgataagaac gcacatgatt tttatacact tgaatgtggt
     7921 gtgggcagtt cattggacga agaacaaact cctcaccatc tcccatatcc attacaggga
     7981 acatatcttc ttgataatga tcccaatgac ctgatgtctt gtacaattct acagaagcaa
     8041 gtggtggtgt gtaaacgtgt tggtagcctg atgctaattc tttatctgtg atgtagcgct
     8101 caagtgtacg acgaatagtc gctccatctg gtaaccaaaa tggcaagcct tggccaactt
     8161 cttggctaat catgaacaag tcaagctctt taccaagctt acggtgatca cgttctttag
     8221 cttcttctag acgagtcaag taagctttga ggtctttttt atcaaaccaa gctgttccat
     8281 agatacgttg catcatgtta ttgtcgctgt taccacgcca gtaagcaccc gccacattga
     8341 gcaagtggaa gacttggata cggcctgttg atggcacatg agggccacgg caaagatcca
     8401 caaattcacc ttgacggtaa accgtcaagc cagcaccagc atgctcatta atcaattcta
     8461 ccttgtaagg atcatctttg aacaattcca aagcttcttc tttggtcact tcttcacgga
     8521 tacatgggta attttctgtc acgatttttt gcatttcagc ttcgatacgt ggcaaatctt
     8581 cgtttgaaat ttggccttca gcattatctg tatcatagta aaagccttct gcaatggcag
     8641 ggcccacacc caaatgaaga tttgggaaca agcgttttgc agcttgggca aataaatggg
     8701 ctgcagagtg gcgaagcacc tcataagcat ctttgtggtc aggtgtcacg atttcaatac
     8761 tgccatcttc ttcaatggca cgagtggtgt caatgagttg atcattgaac ttaccagcta
     8821 aagctttttt agccaatgat ttgctgatgg actcagcaat gtcaaacgtt gttacgccag
     8881 attcaaattc acgcacggcg ccatctggaa aagtaatctt aatcatttgc tttctccttt
     8941 aagtcagtta agatttccac taaaacatgg atagtaagac aaattaggga caagaggtta
     9001 gtgaatagct caataccacc acgtagaaga acaatacctt attcacgaat ctaccctccc
     9061 tagtccttaa aaacaaggct aatcccctcc aaaacaaaaa aactgaccgc atcccaaagg
     9121 acgcaatcag cgtggttcca ccttaattta tgcaggacaa aaaacatgcc ttgcacctct
     9181 agtggcgata agggggccac ccttttatgt ttgcataaaa accaggaagg agtatcagat
     9241 tagctatcct tgtgctcttt ccaccaacca gcactcgctt aaaagattct gtccaatcta
     9301 acttgtcttc aagataatta tagcaagttt tcaaaaattt acaagtctta atccagaaaa
     9361 tctttgatag catggaccag cttgatagga gccttgacta cttttactga ggtgatagct
     9421 gtgaccttag cagcacgttt aggcaagtga atagcacctt gcactaattt tagagaacta
     9481 cttttaccag aatgattaag ataaagactc agcttttgag cttctttgct atttctagca
     9541 attaacgtat ctaaatagaa cgtgtaaata gattttccaa agtgctgtgc tgagatttca
     9601 taacgttttt ttgctaataa ccgtttatcc ataactggtg tttttagtat ggcatcaata
     9661 atagcatcag ttaaatctgt ttcagcgtaa taaagagtgc caaacatttt atcagtcacc
     9721 acatcatcta aataaggatt gccatgagca ataataggag tgccactagc caaactttca
     9781 atataggtca agccctgagt ctcactagtt gatgctgaga taaagaaatc acaagcctta
     9841 tagtacagag caaccttatc atgaggcacc atgcctgtaa aggtcacgtg tttgtcaacc
     9901 tctaactgca tcgccaagtg tttcaaatct tgcaaatagg ggccatttcc tacaataata
     9961 agctttatct tggcattttc agccaaaata gctggcatct gattgatgat agcttgaata
    10021 tttttttcat aagaaatccg tgacaaactc aataacatgg tttcatcacc agcaatgccc
    10081 aattctgctt ttaagttggt tacttcttct gctgtgatgt catcacgaat atatttttcc
    10141 aaaggaatgc ctgttgggat aacccgctta gggatagtaa cttcgtaacc ttctagaaga
    10201 ttgaggacaa tcctacttgg gcagataacc ccatccaaat ccttcaaata gcccctaaga
    10261 agaggtttga ccatacttgg tcgaatgatt tttccgttgg caatataact cacgtagtcc
    10321 tcatattggg tatggtaagt atggacaaca ggaattcgca aagctttgcc tatcattttc
    10381 cctaataagc ctaagctaaa ctcagtttgc gtatgaataa tatcaagatt atagtgtttt
    10441 gcaattttgt atgacgaaat gaggccacga taaaccacac gtctatccgt aaatgacaca
    10501 aaaggaacac ttggcagtcg aataatggtc gggtcttcaa agcgtttgac atctctatca
    10561 gtagtggtga aaatataaac ttcgtgacct tctttttcta gctcttcttt taacgtacga
    10621 atactagtag cgactcctga aacttgtgga aaataggtat ctgtgaatag acctatacgc
    10681 atcttataac tccattactt ttttatagac atctactaag gcatggccaa ccgtttctag
    10741 gcgacgactc tgggcaacct tgtaaccagc ttcaactttg ttgctggcac cagaaaagac
    10801 ttttttcaga gcttctataa aacctggtat atcggttgct aattccgcac tactttgatc
    10861 aacccatccg tagtaaactg gtatatcacg taaaacaagg tgctggcgac tggccaaggc
    10921 ttctaagaca acaatgcctt ctgtttcttc acgacttgga aagaaaaagg catctgcacc
    10981 agtcatggca ccttcataaa catccccttt aatgtatcct gggaaaataa gatttttcgg
    11041 gtggttacca ttgaccattt ggcgaacttg agcaggaatg acccatttgt tggtctcgcc
    11101 aaaccagata aaacgaacat ctggcatagc ttgggcaact ttgacaaagt catcaattcc
    11161 tttcctcaga aaaaataatc ctgctcccat aaccactttt tcaccctctt taatgtcaaa
    11221 gtagcgacga aaagctgctt cctttttagg atctgctccg tactgctcca aatcaatccc
    11281 atttgacact gcaaaaatag gtgacttcac tccataactc tcaatcaaag acttagaata
    11341 tagggtaggg gtaatgatag catctgcctt attgtaaaag tgacaaaggt attttttaaa
    11401 ccaaggagat aatagatttg aaaaaataaa agaatttcta aaatcttctt ctgtagaatg
    11461 cccatgcatg ataaccttct taccagcttt ttgtgctttt atcatcagca gccaactttt
    11521 taaaccatag gtattgagat gaaccaaatc ataagtttct cttggattag tcgtaaaatg
    11581 ttgaccaaca agtgacaagg ctttagcctg atgcttaatc gctcgaccaa ttcctgattt
    11641 tcttagataa ttttctgctt ctaaatacaa taagactttc ataagttcta ttatagctca
    11701 ttttatagta tttttcacta agacatgcaa aaatcagccc taaggctgat tttagtattg
    11761 aagaaacagt ctattgtgat actttttgtt agatggtgct cataattcac agttacttag
    11821 ttgtcccacg ttttttaata ccatgattca aaagaatttc tttttcttcc aactcttctt
    11881 tgttcatgat tttagtcaac atccgcatgc taacagcacc taagtcataa acaggttgac
    11941 tgatagaact caaattggga cgagtgtatt gaaccactgg tgaatcgttg ctggtgatga
    12001 tttcaaagtc ctctggtacg cgtttacctg cttcaaacaa gccgtttaaa aggcccgctg
    12061 ccaattcatc ttcagccaca taagccgctg ttgctcctga gttaatgacg cgctgcgcca
    12121 attcaaatcc ttctttataa gaatagttcg cttcaaaaac aaggccttct ttgaagtcaa
    12181 gcttattgtg cttcaaccct tctttgtaac ctgctaagcg cactttacca ttgatatcat
    12241 caatgagtgg tcctgacaca aaagcgatac acttatgatt ttcagctaaa atatcaacaa
    12301 cgtttgacac cgcagctcta tagtcaatgt tgacgcttgg taattgatga tcaagatcta
    12361 cagttcctgc caaaacaact ggagtacgag agcgtgaaaa ctcggcccgg attttttctg
    12421 tcaagtgatg ccccataaaa ataataccat ccacttgctt ggcaaaaagg gtattgacaa
    12481 cattaacctc cttgtcatca tcttcatcac tggaggcaag cacaatatta tatttgtaca
    12541 tagcggcgat atcgtcaata cccttagcta agatagaaaa ataactattt gcaatatttg
    12601 gaatcacaac gccaacggtt gttgtctttt tgctggcgag accacgcgcg acagcattgg
    12661 gacgatagtc aaggcgatca ataacttcta aaactttctt acgtgtattt tccttaacat
    12721 ttttattgcc attaacaaca cgactaacgg ttgccattga aacaccagct tcacgggcaa
    12781 catcataaat tgtaatggta tcatctgtat tcatttttta tcttcctttc tatttgaaaa
    12841 tgacgctttc aaaaagtatt ttagcatgat ttgtaaccac tttcaagtgt tataggttaa
    12901 tttttgaaaa agttttcaaa aattaactcg aagcttgatt ttttaagctt tttttgaaaa
    12961 aatagtaagt agaaagaagg ttagcctatg acaaaattag accaaatacg tttgtatctt
    13021 gaccaaaagg gagctgaatt ggcgattttc tcagatcctg ttacgattaa ctatttaact
    13081 ggattcttct gtgacccaca tgaaagacaa ctgttcttgt ttgtctacca tgatttagct
    13141 cctgtgcttt ttgtacctgc ccttgaggtg gccagagcta gccaggccat atcatttcct
    13201 gtgtttggtt atgttgactc tgaaaatcct tgggaaaaga tcaaggccgt tttaccaaat
    13261 acagcagcca aaaccattta tgctgaattt gaccacctta acgtcaataa gttccatggc
    13321 ttgcagacta tcttttcagg acaatttaac aatctaaccc cttatgtcca aggcatgcgc
    13381 ctcgtcaaat cagctgatga aatcaataaa atgatgattg ccgggcagtt cgctgataag
    13441 gctgttcaag ttggttttga caatatttcc ttagatgcta ccgaaacaga cgtcatcgct
    13501 caaattgagt ttgaaatgaa aaaacaaggc attcataaaa tgagctttga cactatggtc
    13561 ttaactggaa ataatgcagc caatccccat ggcattcctg gaacaaataa cattgaaaac
    13621 aatgccctac ttctctttga cttaggtgtt gagaccttag gctacaccag tgatatgacc
    13681 agaactgtgg ctgttggtca gccagaccaa tttaaaatcg atatctacaa tctctgccta
    13741 gaggcacaac tagcggctat tgactttatc aaaccaggtg taacagctgc tcaagtagat
    13801 gctgctgctc gtcaagtgat tgaaaaggct ggatatggtg agtacttcaa ccaccgcttg
    13861 ggacatggta tcggcatgga tgtccacgaa tttccctcca ttatggctgg caatgacttg
    13921 gtccttgaag aaggtatgtg cttctctgtt gaacctggta tttacattcc aggaaaggtc
    13981 ggtgtgcgca tcgaagactg tggccatgtc actaaaaatg gctttgaagt ctttacccat
    14041 actccaaaag agttgcttta ttttgaaggc taagcctcta ccatctggtt aaacaaaccc
    14101 tcgcgaaagg aaggtattcg tatggatcaa accattcatc accaaataca gcaagcacta
    14161 cactttagaa cagccgttcg tgtttataag gaagaaaaga tttctgatga ggatttagcc
    14221 cttatccttg atgctgcttg gttaagccct tcttctattg gcttagaagg ctggcgcttt
    14281 gtcgttttag acaacaagcc tattaaagaa gaaatcaagc cctttgcctg gggagcccag
    14341 tatcaactgg aaacagctag tcactttatt cttttaatag ccgaaaaaca tgcaagatac
    14401 gatagccctg ctatcaaaaa tagcctttta cggcgtggta tcaaagaagg tgatggtctc
    14461 aacagccgcc taaaactcta tgaatctttc caaaaagagg acatggatat ggcagataat
    14521 cctcgggcac tctttgattg gacagctaaa caaacctata tcgctcttgg taatatgatg
    14581 atgacagctg ctcttttggg cattgatact tgccctattg aaggctttca ttatgataag
    14641 gtcaatcata tcctagctaa gcataatgtg attgatttag aaaaagaagg cattgctagc
    14701 atgttatccc taggctatcg tttgcgagat cccaaacatg ctcaagttcg taagcctaaa
    14761 gaagaagtga tttcagtcgt taaataagga gattaaaatg aaagcattac atacctgtat
    14821 ccgcgtcaaa gaccttgacc agtcggttgc tttttacaca agtgccttcc cttttaagga
    14881 aaactatcgt aaagattttc cagatagtca atttactctt gtttatctgg ctttagaagg
    14941 agaaagttat gaattagaat taacctacaa ttatggtcat ggcgactacg acttaggaaa
    15001 tggctatggt catattgccc ttggctctga acattttgaa gcagaccata aaaaacatcg
    15061 acaagcaggt tttccagtta ccgatatcaa agaactggct gacaaatctg cccgctatta
    15121 tttcattcag gatccagatg gctacaaaat tgaagtcatt gatttaaaca actaatacta
    15181 ttaacaacag aagctttaaa gcttctgttg ttttattttt taacctgacg attgggacaa
    15241 tctcattatt aacagagttt tgaaatatct cagcttttgt gttacaattg ccctaaaatt
    15301 ccatttgaaa gaaaagtcta tgacactact atctatcatt gtcccctgct ttaatgaaga
    15361 agcaaacatt ctcccttatt ttgaagaaat gcatcaacta gaaacgtcca tgaccaacca
    15421 acttgctttt gaatacattt ttattgatga tggttctaag gataacacct tgggtatttt
    15481 gcgtgagcta gctgctcgct ttccaaatgt gcattatctg tctttttcac gccattttgg
    15541 caaggaagct gggctcttag ctggactaaa agaagccaaa ggcaattaca ttactgtgat
    15601 ggatgtggat ctgcaagatc cacccgaact tttgccaatt atgtacgcta agctaaaaga
    15661 aggttatgac atcgttggca ccaggcgcca aaatagacaa ggagaaccac taattcgttc
    15721 aatgtgttcg aatctatttt atggactgat caaacactta tcagatactg aaatggtcaa
    15781 tggagttcgg gattatcggt taatgaccag acaagtcgtt gatagcattc ttgagttggg
    15841 agaggttaat cgtttttcaa aagggatctt ttcgtgggtt ggttatcgta tcacctatct
    15901 tagttttgaa aaccaaaaac gcaaatatgg aaaaagtcgt tggcattttt gggagttgct
    15961 ccgatattct ttagatggct ttatcaactt ttcagaaatg ccgttaacca ttgctacctg
    16021 gacagggact ttcagctttt tgatttctat tttcgccatc ttatttatca ttatcagaaa
    16081 gatactcttt ggtgatcctg tctctggttg ggccagcact gtctctatta ttttattcat
    16141 gggaggcatt cagctctttt gcatgggtat tattggaaaa tatatttcaa aaatattcct
    16201 tgaaactaaa aaaaggcctc tctatattat caaagaaaaa cattaaatta aaacattttt
    16261 ctcatcaatc gtttcctact ccttctgtgc gcgaagcttt ctttttaagc ccgttatacc
    16321 aaatgataat gacctttcca aaatagtatc accctcctta acactttaag tagaaaagga
    16381 actattaggt tgagcctttt tgcaaatcct agcgactaat gttataatcg tcatactatt
    16441 gaaaaaggag ttctttatgg agtggattaa attaatcggt attgtgatta tcgtattagg
    16501 gtttatttta aaatgtgatg ctatcgctac cgtagttgtt gcaggacttg ttaccgcgct
    16561 ggtttcagga atttcgttta ttgattttct agatatttta ggaaaagaat ttaccaatca
    16621 acgtttgcta accattttct tcatcacttt accgcttatc ggcttgtcag aaacttatgg
    16681 gctgaaacac cgagctactc agttgattca acgtgttcaa gcccttaccg tcggccgttt
    16741 attgactctc taccttatta ttcgagaatt agcaggtctc ttttcgattc gactaggggg
    16801 acatccacag ttcgttcgac ccttgattca acctatggga gaagctgctg ctaaagccaa
    16861 cattggagaa gagttgaccg atgcagaaaa agatgacatc aaagctatgg cagctgccaa
    16921 tgaaaacttt ggcaatttct ttgcccaaaa tacctttgtt ggcgcaggag gtgtcctact
    16981 tatcgctgga actttagaac agcttggcta tgatggtaac caagctaaaa ttgcattttc
    17041 ttctatttta attgctatta tctctattat tatagttgct atttacaatt acctctttga
    17101 gaaaaaaatg gaacgtcaac atcagaaagg agataactaa tggctaacat tgcaaacctt
    17161 gttttagaaa cgatatatat ccttatcggc ttgcaactgt ttcacacagc ctactgcacc
    17221 tttaaagata aaaccaaccc tgtctatttt gggacagctc tgttctgggg attgctgggt
    17281 gttaccttcg ttggcggtgc ctttttgcct aataaagtta tcggttttat cgtgattgtc
    17341 ctagctcttt taaccttgtt taagcaagtt cgtattggaa cattaccagc ctttaatgaa
    17401 caaaaagctg aagaaagtgc tcaccgcatt ggtaactgga ttttcttacc agttatgtta
    17461 atggctatga tttctctact tcttgccttg atccttcctg atttcagtaa aagtgccatt
    17521 gggattgctg gtatcttagc cactattgct attttaataa tcactaagca aaaaccatct
    17581 gctcttttag ctgaaaataa tcggatgaac caacaagtgt ccacaagcgg tattttgcca
    17641 cagctcttag gagctttagg agctattttt gcagctgctg gtgttggtga tgtgattgct
    17701 tctctcattc gggagattgt acctgctgat agccgcttct ttggcgttct cgcttatgtg
    17761 cttggcatgg ttatttttac tatgattatg ggaaacgctt ttgctgcctt taccgttatt
    17821 acaaccggta tcggtgttcc ttttgttttt gctttaggag ctgaccctat tattgccggt
    17881 gctttagcta tgactgctgg tttttgtggt accttattaa caccaatggc agcaaacttt
    17941 aatgccttgc ctgttgcttt gatggagatc aaagatcgaa atgcagttat caaaaagcaa
    18001 gctcctatcg cccttgtttt aattattagt cacattgcct taatgtattt acttgcttat
    18061 tattaacttt tttgctaagt tagtagcgtc agttattcat tgaaaggaca ttattatgaa
    18121 aattcttgta acaggctttg atccctttgg cggcgaagct attaatcctg cccttgaagc
    18181 tatcaagaaa ttgccagcaa ccattcatgg agcagaaatc aaatgtattg aagttccaac
    18241 ggtttttcaa aaatctgccg atgtgctcca gcagcatatc gaaagctttc aacctgatgc
    18301 agtcctttgt attgggcaag ctggtggccg gactggacta acgccagaac gcgttgccat
    18361 taatcaagac gatgctcgca ttcctgataa cgaagggaat cagcctattg atacacctat
    18421 tcgtgcagat ggtaaagcag cttatttttc aaccttgcca atcaaagcga tggttgctgc
    18481 cattcatcag gctggacttc ctgcttctgt ttctaataca gctggtacct ttgtttgcaa
    18541 tcatttgatg tatcaagccc tttacttagt ggataaatat tgtccaaatg ccaaagctgg
    18601 gtttatgcat attcccttta tgatggaaca ggttgttgat aaacctaata cagctgccat
    18661 gaacctcgat gatattacaa gaggaattga ggctgctatt tttgccattg tcgatttcaa
    18721 agatcgttcc gatttaaaac gtgtaggggg cgctactcac tgactgtgac gctactaaac
    18781 ctattttaaa aaaacagaga tatgaactaa ctctgttttt ttgtgctaaa aatgaaagac
    18841 ctagggaaac ttttcatcgg tctttctcaa ttgtcatctt aatctaatac tacttctaac
    18901 atcagcgggt atagtttgcc agtaattaag aaacgttgtt gatctaaatg agcaatccca
    18961 ttcaaaacat taaggtcagg gtaatgggac ttatcaagat ttaaggcttt taacaaagga
    19021 ctaatatcat aggtggctac cacctttcca gaatcaggtt ggagtttgac aatagtattg
    19081 gtttgccaaa tattggcata gagataacca tctacatact ctaattcgtt aagcattgag
    19141 atggggacac tttctatagc aactagtacg gtatccaata aagcaaaatc tttggggtct
    19201 cgcttttgca aaaaagcatt tccactagtc atccaaagac actggttttc cttatcatag
    19261 gctaatcccc agccatctcc ttcaaatgga taggccccta aacaattgca ggtagcctta
    19321 tcaaacttgt aggctacacc ctctttatag gttagtaacc aaaaataatc ctcaacaact
    19381 gttagccctt ctgcaaaaac tgtatctgga aaagctattt tttctgaaaa gatctcttga
    19441 gtaaggtcat acacccctac ttttgagaaa ccataacgac cggcactaag taatatatga
    19501 ttgttgttga gttgttctaa tccctgcgtg tataagttgc tatcataaga ataagttcgt
    19561 aacaaacgaa cttgtccttt agttatcata atactcattc accttacctt gcattgtagt
    19621 gtttcatttg ttttttaata tctctttcct gatcacggcg tttaatggtt tcgcgtttgt
    19681 catactcgtg tttcccttta gctagaccaa tcagaacttt agcaaagcca tccttgaggt
    19741 aaactttgag aggaactaag gtcatgccag atcctttaag ttcatttgct agatgcgtaa
    19801 tctcacgttt tttgagcagc agtttgcggg ttcgttcagg atcagcgttc caaatattac
    19861 cttgttcaaa aggtgcaata tgcacgttga ccaaccacgc ttccccattc ttaatttggg
    19921 caaagccatc tttcagttga atacgagctg cacgaacact tttaatctca gttcccgtta
    19981 agacaatccc tgcttcaacg gtttcaacaa tgtgataatc atgtctagcc tttttatttt
    20041 gcgccagtat atgaccttca ccttttgcca tagttaactc cttttacgac tcttcttttt
    20101 tgcttgttct ttgtaaaatg gttttttaga ccctttttta gttttaccct tggtttttgc
    20161 cttggctact tctttaggtt cttttttctt tgtcccttta gatgattttg aggatttacg
    20221 gcgatctcgt cgactagctt tatccgacat cttgattttt tcaaccacat caaagtcgct
    20281 aggtaaatac tcaaagtcaa tgtcaccagt ttccttgtca gccttaacca atttcacacg
    20341 aataggttga ccgaccttaa agaccttacc tgatttttca ccttgaaggc ttagcgttct
    20401 ttcattaaag tgatagtatt ccggtaagct agtgatatga accaaacctt caatcgtatt
    20461 tggcaattct acaaaaaaac caaatttaac gacactggaa acgataccgt caaattcttc
    20521 gccgacatac tctgccatat actcagcttt tttcatggct tcaaccacgc gttcagcatc
    20581 aatagcacga cgctctaact gtgaagaaga tgttgctaat tctggaatga tctgagcaaa
    20641 atggtcgcgc ttttcttgac taggttgatt gtactcacga accatgcggt gaaccaataa
    20701 gtcagggtaa cgacgaatcg gactggtaaa gtgagtgtaa tattctgcgg ccaaaccgta
    20761 atggccatga ttatgttcag aatacctcgc ttgttgcatg gaacgtagaa gcatcatgtt
    20821 gagcacttct gctcctggtt gaccttcgac ttttgccata aaggcttgca aggcttcctg
    20881 agaaatcttg ttggctgttc cttgaatgtg aataccaaag gtgctagcgt aatcaataaa
    20941 ttgttgaagt ttttcggcct ttggttcttc gtgaatacgg tagataaacg gtaacttagc
    21001 ttttgcaaaa tgctccgcaa cacactcatt ggctgctagc ataaaggatt caatcatccg
    21061 ctccgcaatg cccctttgac gaagcaccac atcaaccggc attcctttgt cattaacaat
    21121 aatttttgct tcttgcgtat caaaattcaa ggcgcctcgt tttgaccgca tagcttccaa
    21181 aatcctatgc aaagccacca tgagggtcac atcatcagca atgctagcga attcttggag
    21241 cgcttcttca tctcctgcaa tcatgtcatt gaccgtacta tacgtcattc ggtaagttgt
    21301 cttaatcact gattgacaga tttgataatt gaccacatgc ccttgagaat taatttccat
    21361 caaggctgac tgggtcaaac gatcaatgtt agggttcaaa gagcagatgc catttgacaa
    21421 acgctctggt aacatcggaa ccacacgatc ggtgacatag acagaagtgc cacgggcgat
    21481 agcttcctta tcaagggcag atccttctgt cacataatat gagacatccg caatatgtac
    21541 cccaagttca taattcccgt tatccaatag cttaatatgg atagcatcat ctaagtcttt
    21601 ggcgtccgca ccatcaatag taatggtggt ttcttgacgc aaatccacac ggcctattaa
    21661 atctttagca gttggtgctt ctgaaatagc attggcctca gctaaaactt ctgctggaaa
    21721 ttctgacacg atatccatcg attcaaggac ttctaggaca tcaattccaa cgtctccttg
    21781 atggcctacg atatcacgca cactggctac aaaataatcg tgcccgcgta tcgggtattt
    21841 atcaatatca accttgatga tttcagtgcc tttaagcaca acaggttctt ttttgatata
    21901 gattttttgt tgaatctttt ggtttttgga cctgatataa ccagcatatt ttggtttgtc
    21961 atcatctaaa ataaaggttc caacagctgt ttttaaagaa cggtcaacaa tagcaacgac
    22021 cttagcttca gccgctgtgc ctttgagacg gtctgctggc tttttaacca ctacttcgac
    22081 agtatcacca tcaatagcat acccaacatc attacgaccg ataaacatgt catcttcatt
    22141 ctcatccaca tgtaagaaac caaagcctgc tttattggca cgaaaaacac cctgaacagt
    22201 aggttctttt ttcttttcac gctcttttct aagagccaga ctgccatcat ctgaaaaccg
    22261 taacaggctc tgactttcca ttttggaaat ggttttaata aggctgggaa aatgcttagc
    22321 tccagccatc tccaaggccg cagccaagtc gttcacattg gacttgccat gctccttgag
    22381 gtaagtcata attctttctt tcatcatacc taatttctat ttctgttttg tttgttggag
    22441 gatatcaatc cgtttcgctg ataaaaaaat gagctagact aactctgtca agctcactcc
    22501 ttatttactt gataaaatgg caagcacaag tgcaatcgct agccaaaaaa atactagaac
    22561 tgctgtgaat cgttgcatga atgcttcaaa gccacgagct tttgtacgtt caaacaaggc
    22621 ttctgaaccg ctgctatcaa aaacattgct acttgggttt ttttgaggtt gcataaaaat
    22681 agcaatcaca agcaataccg acagtactag caatatcgta agtagtaagt tgtacatgtt
    22741 atttcctcca ctattgtcat gaccattcta acacaattct tatcaagttt caacatgcag
    22801 ggtcactttt ctctccttgg ggcagtattt gggaactttt attttttccg gatgactgct
    22861 tttattttta ctggttagat aattgttact gccacactca ctacattcta aattaatttt
    22921 tactctcaca caatatcctt aactttaata tattttcgaa atataatcaa actccatatt
    22981 aaattaacaa acacaatcaa actagtcaca taaaaaacag aacggtagcc taacactaca
    23041 gccacatttg atccaataaa agggccaatg acttgaccaa ggttactaaa catttgatta
    23101 taggcaaata ctctagaaat accttctttt ggtgttaact tagttagtaa agagttaata
    23161 cttggcatca aagcgcccac accaaaccca taagcaaagc gtagtactcc taattgaaaa
    23221 ctggtctgcg ctaaggcact gctaaaatac atgataaagc tataacacaa agcggctaac
    23281 aataaccgat gattgccaaa tcgatctcct aattttccta agtaagaact gctaaataag
    23341 ctagaaaacc ccatggccga aactaccagg cctgaagtaa acattaagtt gtgagtttga
    23401 ccaagatgac gaatatacaa tgataaaatg ggggccactg attgagctga aatttgaata
    23461 atcatactag tcacaaataa accaagcata atctggggac tttttacctg ttttaaaatg
    23521 acttttgtag gtatcatctc aaagcgccta accggcttaa actcttcttt aacataaaca
    23581 gctgtcatta aagaacataa aaataatatc acaccaacta ataagaaaac ttgtcgaatt
    23641 cctaatagct ctgccaaaat tccccctagt aaaggaccaa ttaaagcgcc tgctgtcact
    23701 cctgttgcta atgtgcctaa agcataacct gactcctctt ttggggcttg gctggcaatc
    23761 aaggcagtgg catttggcac atagcccgca aagacacctg ttaataatct caaaatgagt
    23821 aaccaaaaca cattaggaat aatagctaaa ccacccattg taaatgtcat aacaaaactg
    23881 gctcgcagca tcataggttt tcggccataa cgatcagcca acttccccca aacaggagca
    23941 aataaagctg aagcaagtgc cgtcacagca actgccagac cagcatagta ttctactaat
    24001 tctgtcggag tacctaaatt ttccacatat agagccataa aaggcataac aagtgaaaag
    24061 cttgccccag taaagaagtt accaagccaa gccactttta gattttgtcg ccagttcact
    24121 gtcgttaccg ctttatcact aatagatcat catcttcttt ccattgggcc tttgattatt
    24181 ataacacatt tctgccaaaa acagtgtccc ataatagtgc taagtctcat ctgatagcct
    24241 tcatcgttta cgtaaactac ccagtaaacc tctgaccagt tctcgcccaa ttgttctaac
    24301 cgtcgtacta ataaacgcat ctgttgcttt ttcgacgact gttttgtggg aacgcccaac
    24361 agaacgccga ttagcttttt tggtagctgc ttgcgccttg atggcttctt tagcttcttt
    24421 ttcgcgttgt gctgtcgcta tcgcttcttg ggccagtcgg ttgtcttcta aaaccttagc
    24481 agctaatttt tcataagcag attcacggtc aatggactga ctatatttac tggcaaaagg
    24541 tgaggactgc acaagctgct gactctctat ctctgacaaa acagcaaagc tactcttagg
    24601 cggcatgata taggcacgtt caacgatgga tggttgtccc ttatcattca aaacagaaat
    24661 caaggcctca ccgacttcta gttctgtaat cacccttgcc acatctaagt caggattttg
    24721 tcggaaagta tccgcagcga cacgaaccgc cttttgttct ttcggtgtat aagcgcgaaa
    24781 ggcatgctga atacgatttc caagctgagc caaaaccgtt tctggcaaat caagtgggtt
    24841 ttgagtcaca aagaaaatcc caactccctt tgaacgaata agtcgcacaa tctgttcaac
    24901 tttttccaag aaaactttag gggcatcttt gaagagcaag tgagcttcat caaaaaagaa
    24961 aaccatctta ggtttgtcca aatccccaac ttctggcaat aatttgtaca gctctgataa
    25021 taaccaaagt aagaaggttg tgtaaagggt aggagattga aaaagcttag tcgctgacaa
    25081 aatgttaatg gctccataac ccgaagcaac atctaattgc atcaaatcag ccacatctaa
    25141 agcaggctct ccaaaaaatt ggtgggcccc ttcttgttcc aaagtcaaga gacttctttg
    25201 gatagccccg atagattgct tagcgatatt accgtaatgg ctggagtagt ctgaggcatg
    25261 gtcaccaact tcttttaaga tagcctgcaa gtctttcaaa tcaatcaaaa gccaaccttt
    25321 ctcgtcggcg attttaaaga cgatgtttaa gacacctgtc tgggtatcat taagattcaa
    25381 taaacgcgac aacatcatag gtcccagctc agaaatcgtt gtccgtaagg gttgaccgtt
    25441 ttgaccaaaa acatcccaca ttcttacagg aaaggcctga ggctggtaat cacttacccc
    25501 gattgtggct aaacgcgcag ccaatttatc agtcacctct cctgctttag taagattaga
    25561 caggtctcct ttgatatctg ccaaaaagac tggcacacca gctaaactta attgctctgc
    25621 tagcactttt aaggtcacag ttttacctgt tccagtcgct ccagcaatca aaccgtgacg
    25681 atttagcatc cctaattgta aggctatcgg ttgattgcct tttgcaattg tcattgtagt
    25741 cataggcttc ctcttttttg atgatcatac ttatcgatat gtgtttaccc ataagttaaa
    25801 gtgtttagaa gggtctcact cttaatacta ttataacata taacccttga tgcgctaatt
    25861 taaaagggac cagctagttt tagacagaaa tagcttgatt ttgacagcta tgatacgaat
    25921 ctctcagctt agctacctcg gattggctaa taacaacaac gcagattgta cttgctttat
    25981 taaggctgca atatccccac tattatcaat cactagactg gcatagggct ttttttcctc
    26041 tattggtaac tgactggcta ttcgctgcct tgcttttcct ttgtctaaac gatttctagc
    26101 cattaagcgc tgcaattgtg tctgagcatc tacataaacc agccaaatcg catcaaacca
    26161 gtcttggtag cccaattcca ttaaaagggg aatatccatg aaaaaaatgg cttggctctg
    26221 tgctaagtgg tcacgcttag ccgccaattc ttccttaatg atttgatttt gaatggctga
    26281 ggatgttgcc atattatctg ggtttgaaaa aagcatttct gacagctttg tgcgatctaa
    26341 ctcaccatct gcttttaaaa tctgattccc aaaagcttct ctaagagcct cgtaaagacg
    26401 accgcctttt tcttgcaagt catgaaccac ttgatccgca tcaatcacct gataaccagc
    26461 ttttctaatg accttaacca cagtcgattt tcctgatgca atgcctcctg tgatgccaat
    26521 aatcatagtc tttttttctg gcattttgga caaatgtgcg tcccccttcc tccaactttt
    26581 aatttgacaa tagcctgtcc acaacgagga cacggtttcc cagtttgacc ataaacctgc
    26641 aaatagtcct gcatggtgcc atcagctccc aaagcattac ggtaagtacg gacggtcgaa
    26701 ccgccctttt caattcctaa agctaggatt cgtatggttt catcatgcag ccgttttatc
    26761 tccgccttat tcaacctaga agacgcagtt tctggatgaa ccttagcagc ccaaagtacc
    26821 tcgtccacat aaatattccc taaaccagca accaaggtct ggtctaaaag atgtggtttt
    26881 atgggttttt gggaggatag cagagcagcc tcaaaggttt ttaacttaaa ggtctctttc
    26941 tttggttcag ggcctagttt tcgtttggca aaaaacgctg ataattgaga ttttgctatc
    27001 aaatcaaagg ttccaaattt acgcacatct tggtaaacca aggtcgaacc atttttcaat
    27061 tcaaaaaaga catgaaagtg tttgttatca gggacctcat ctggaaaaag caaatatttg
    27121 ccttccatcc gcaaatgtga taccaacact aattgcccca aatcaattaa taaatatttt
    27181 ccccgacggc caactgactg aatgatttga ccaggtaggg tcagggcaaa cgtctctaag
    27241 tcggttttaa ccatttttgg cactttaagc gtaacagcca caatttcttg gccaagcact
    27301 aaagtctcaa gtcctcgtcg gaccgtttca acttctggta attctggcat aatccgttat
    27361 ttaaaggaca gctagaccct ttgctcctac aaagaataaa ctcagtataa aaaagtcact
    27421 caatagactc aggtctaggc tactgactgc gtttcaaaaa acacagctgt ccttctcctt
    27481 tcttatttct tattttacga acacatctga tagaaagcaa ctcataaatc tgcctcagac
    27541 cagtcctcat cccccttacc tcccagcact atttgacggt aatgttcctt ataactataa
    27601 tacaatgagt cctctccaag atacttaaaa atctgcaagg cctgccgcat gtcctcttct
    27661 ccagacattt cttcctgctt taatttataa tacccatgca catataagaa aacagtcttt
    27721 tcataaaaat tgagctcgtc tcgcagcaaa agatcaattt tgttaatcaa atagcgacta
    27781 tgttcaaagc aagaatggtc aatactgata atgagacaat taatggataa ctgagtcact
    27841 aacattttat tggttttatt ttgttccgaa taagcaaaag atgctaccat ttccttagtc
    27901 aataaaaata gggtattgta attcatgaag cgcgagcaat tccccagaag aatgatttca
    27961 taatatcccc actgttccac tttaaataaa taatctgtga ggcgagtcaa ttcttcttga
    28021 ctaggtgcaa ttgaggagtc aatagaaaaa agaatagctt taatcatcgt tcgttcataa
    28081 tctttatggg cataatcctt caacagtttg gtcaatttaa ccacgttttt ctcagcataa
    28141 cattttctcg cctgacttaa gagcgtgaaa aaatgcgtat gcgtcttgga atgagccgaa
    28201 acaaattcgt caatcgtaat attcagttta tccaaaagat taagcaagcg actgcaggtg
    28261 atttctgatt cccctcgttc aaatcttgaa atttgtgact tggacaggta ttcatctgct
    28321 aaaaaagaaa tgctaacctg cttaccctta cgtaatcgcc ttaaggtttt tccgagttct
    28381 ttttccattt ttcccacttt cacaacaaaa aattaaaatc tgctaaatcc attatataat
    28441 acaagtacaa aatagaaaat cagaaaggag acagtcatga aaaaagttaa taaagctttg
    28501 ctttttacat tgatcatgga tatcctgatt atcgttggtg gataaccttt cgcttaaaaa
    28561 caatccactt ttgggaaacc cttatattta gtttgtaaca ggaaaactaa taaatattaa
    28621 aaagagtgtt agcgacctag cggcagcttt actcttaagt gcgtttgctg ccattgacag
    28681 caaggtcaag gctgatgaaa cagcaggggc ataccgttat gatgtgtctg gacaaaacac
    28741 ttactatcaa actatcagct ccattaacca gcaacgacct ggtgccactc tcgacgaatg
    28801 aggtgattat tatggaaccc ttacttcatt tacttcccaa agtcgtacca gtacttatac
    28861 acttggtaga caaagccaac gtatcatggt taacagtgat attacagtta cctatactct
    28921 taaagacaat ttggtagccc ttcataagga atataaaacc tttgtcaaaa aaatattaca
    28981 gataaaccga tcaccagtga ctacgacaag gctaaggtta tttataacca tttaatcaac
    29041 gaatacactt acgccacaaa ataacttagc accaattgca aaaccgtaag cagcatcaac
    29101 aatcatactc ctaaagctct ttatcgtgac aagtgtggtg tttgtcaagc ttatgccgtc
    29161 atgtttaaag atatggcaga agctgcaggc ttatcagctt ggtatgtgat aaaaaaggat
    29221 tgaatgagca ccatgcttga aacatcgtta ccattcatgg tgttaattcc tacgttgatg
    29281 ctacctggaa tgattcagca tacaccacaa ggtatttcct tgctggaaaa gacgtgagga
    29341 atactgagta ccgtttagat agccagtaca accacttggc taatgctatt ttaaatacca
    29401 gctaccaaaa aggctaaact aacaacaact acatttttca aatagaaagg cctaaacaat
    29461 gaccaaaaaa cagttttatc ttatcttagc tattagcata ctgtgtttta ttcttgccgt
    29521 cagccctaaa ccaccaattc ttaaacacac tattgaaagg aaaaggactg cgatgaccat
    29581 aggccactca aaacaaaacc ctcacaaaaa agaagccctt aaacacaaaa aaaacaaaaa
    29641 taaggctctt tttcttccaa gtggggacag cgctatcaaa tctgttatta gcatgacagt
    29701 tagactcctg ttagatgacc aatcttagct ctcctaaaat cagtggacac taccatttcg
    29761 gtagtgttct tttttaagct aatctcatca ccacgctttc ttcaagctaa aaaactgatc
    29821 atgaaagagc aaccccatca ccctaacagc ccactttgcc cgcactttct taaaaagtta
    29881 ggagaaaacg atataccaat tattttctga tcccagctcc ctatcattaa caagatgtgt
    29941 agcacggacc tacatacagg taagctagag tcattctcgc tatttcggac aaaaagaggc
    30001 cggtagttcc ctgacctctt ctttatttat cttgtaacac tcctcttctt agcgagactt
    30061 actagcacat aattcttaag cactcttatc atttagacta ggaataacac catctgaatg
    30121 aagcaaatga tgcgtcagat ctgccagagt atcatctcca taagtaatcc ctgtttggtg
    30181 ctgtttctga tgaaaatcag atgactcctt aactttagca gtcacctgct ggtcacttgg
    30241 atgaaaatta atttctctct tggcttcagc cgcaactggc tcgaggaaca acttattgac
    30301 atcacggtct tcctccaaat gattaaggat agcagcatca tcttctaacg aaagaacctt
    30361 cttgggtaac aaaaaggggt tatttagcct tggctcagta acgacttcct tggctactcg
    30421 tgatagttta ttattgtctt cctcaggtat caaacggtta ggcaatcgat gcaattcttc
    30481 cggctgtgag gcgctcacag ctacttcaga agaaacctcc aagccctttc ctgtttgcct
    30541 gtctaaagca accggcaaat tattaactaa ctttttaacc tgtcctcagc gctcatttca
    30601 acactaggaa gagctgtcgc tggttctagc cagctaccaa cttctgctgg tcttacctta
    30661 acatcgctgg caggcttctc cttctcatct tcattaggtc taaaaggctc aaaccgccct
    30721 gttgattttt catctatgac ctttatattg tcttttacta catgagacag tggttcgaca
    30781 tttaccatag cttcatgctc gttaaggctc tggtctcgtt tgaatttacc aggaatacca
    30841 ggagtcggtg ttccgacaga ttgatcggct tcagtaggct cttttacgga gccttttttc
    30901 cataaaactg ctggaggaat aatatccgta gagccgtccc cataagtaat cgtggcctgc
    30961 ctcccctttg gattaataga tactttaata ttgtctggga aagccccact acctcctgtg
    31021 gttaaaaggg cacccgcatt tttaatgaga gttgtcattc gttcaatacc agcaacgcca
    31081 atatggttca agtcatctac tggtaactga tcaacagatt tcatggtgta tttccaagct
    31141 tgcggttgaa caagaaaaac agttccttta tacattgtgt tatgcatatc atcaatgact
    31201 ttgaaatacc ttatatattt ggtttgtaca ttagggttag caacgggaac agtgctttca
    31261 ggaactcctg acatcgtaaa aatccctcta gcagaagttt gaaagccctc ataaacatga
    31321 cccataccag gaacaatctg agcggtcaaa gacttatcag aaccatccaa agcaatgcga
    31381 atatctttca tcagagacat ctgattggta taggcaaacc taaccgtgtt agcctcacct
    31441 cgatacaaaa ccacttggga gggctgatcc gaagaaaact gagaggttgt ttcttcctta
    31501 tagccaactc caaaagccaa ctccaaaacg tctatcagac gaatggactg aatctgccgc
    31561 aactgtacta ctcaaactga ctagtaaagt agccgcacaa ccaactaata aactgctcgt
    31621 tttaacttta acatctctta acatcatcat gttcctttca ttatttttta tttaggaatt
    31681 cgacatacta tattttctca tattagtcta acaaaaaact cgtaattcta tataaatcat
    31741 ataaaatgac gagctttggc aaagatgagt atagcaggat ggaaaacaga ttatttctga
    31801 aacttcaaag gtatcttctt gtcacgaact attctttaat tgaatcggtt acactaaact
    31861 agtcccatta tttaaaaaca aactttaaat attattcatt tcacttttta agtttattaa
    31921 atatagagtg attaggagaa ataagacgac ctaatgataa aatcattcct attgtatagc
    31981 aaacaacaat atctaaagtt ttcataccac tcctctacta cttatacatc agtgatagat
    32041 agtcatttat atatttagtg aggccagata atatttgtag cagcaccagc tgctccacac
    32101 aaaatataac cttgccaagg gataaatacg cctgtctgag cacaaactgt cactccctgc
    32161 gcagcacctg ccatccctgt caaaaagata tttgctccgt cttttccgcc tccatttatg
    32221 atagccagac cattttttag cacttccata tttttgaagt ctttttcatt ttaatttcac
    32281 tcttttgtat tttaaaaagc taagagtgat atctaaattc attggcataa tcactatttt
    32341 cttttatcga taaaaacaaa taatgacgct accaaagcta cgatagataa catgtttacc
    32401 gatttttccg aaagggataa ttgattgaaa attaaaaaca ctaaatatgg tatagattta
    32461 tcaacatttt tttatgaatg gcatcaacaa tcattaaaaa cattatagac gcatgcataa
    32521 taataaaaat cgtatagatc atttaatttg ctgtatatcc caaatatgct ccaccaactg
    32581 taaatcctac aacacatcca cctgtcaata ctggatgtgg cagtttacaa aatttcaatc
    32641 cagaagctaa gcctccaaga ccaccaatta cagcatctcc tggagcgttt cctccactta
    32701 tactagacat gtcattaatt gttaattcct tcattgttgt catcctttcc ttcctctaga
    32761 atccgcctgt agctcctgtt acaccagccc aaagagtaat agctgtttta gctccaggct
    32821 acaccgcaaa ctggaaaacc tatagcagct ccagcactac ctgcagcgat aaccccactg
    32881 acatttgcct gccaattatt cttaccaccc tcaaccttgg caagcttttc tatagtcatt
    32941 tggtgaaatg tttccagttt ctttatctcc atatcgaata acttcttttc ataattctta
    33001 aatttgtctc gttgcaacct cgagctagta cagaatatca taaagaacag tatcatggtt
    33061 tcaaaatctt aaaaggctat ttttcatcat gaaatgtctt tttatcctct ctacacttac
    33121 gctgtgacaa tagttaatta attaagaagt ttaaatgata ccattcatgc ttttataaat
    33181 accttttaag atttttgatg gtagtgtaaa atatcttgtg taaacacaca aaaaggaata
    33241 aatcctgtat agtagagttg cgacacttac tagaaagaga tttattccca tgactcagtt
    33301 taccacagaa ttacttaact tcctagcaca aaaacaagat attgatgaat tctttcgctc
    33361 ctctttagaa atagctatga atgacctctt gcaagttgaa ctatcagctt ttcttggtta
    33421 tgaaccttat gaaaaagaag gttacaacac aggtaatagc cgtaatggga cttattctcg
    33481 tcagtttgaa acaaagtatg gcttagtcaa tttaatcatc ccaagagatc gaaacggcga
    33541 gttttcacca gttttattac catcttatgc tagacgagag gaccacttgg aagagatagt
    33601 gatcaaactc tatcagactg gcgttacgac acgcgaaatc agtgacatta ttaagcgcat
    33661 gtatggtgac cacttgtttc tgagtggaag cctcgttaca aaaaggttat gacatcactt
    33721 gaaacaacgg aaaatctcct aactttctat caatttccac attacatttg gtctagtatt
    33781 tactctacaa acttgattga atcactcaat aaagaaatca agcgacaaag caagaaggag
    33841 ggtggttttt ccaaatgaag aagccttaga acgatgtctt gtaagtattt tcgaggacta
    33901 taatattaag ttcggagctc gtattcataa aggattcgga gcatgctttg acacacttga
    33961 tag
//
LOCUS       DAXWIQ010000016        32720 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000016 DAXWIQ010000000
VERSION     DAXWIQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32720
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.016"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(143..2308)
                     /gene="pbp1a"
                     /locus_tag="ACGEUI_000775"
     CDS             complement(143..2308)
                     /gene="pbp1a"
                     /locus_tag="ACGEUI_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1A"
                     /protein_id="HFF8165628.1"
                     /translation="MITIKNPKILKWLKYVLSAILSLIILVIIIGGLLFTFYISSAPK
                     LSEAQLKSTNSSLVYDGNNNLIADLGSEKRENVTADSIPINLVNAITSIEDKRFFNHR
                     GVDLYRIFGAAFHNLTSQTTQGGSTLDQQLIKLAYFSTNESDQTLKRKAQEVWLALQM
                     ERKYTKQEILTFYINKVYMGNGNYGMLTAAKSYYGKDLKDLSYAQLALLAGIPQAPSQ
                     YDPYLHPEAAQNRRNVVLQQMYMEKHLTKAEYETAIATPVAEGLQSLQQRSTYPKYMD
                     NYLKQVIEEVKKETNKDIFTAGLKVYTNIIPDAQQTLYNIYHSGDYVYYPDQDFQVAS
                     TIVDVTNGHVIAQLGGRNQDENVSFGTNQAVLTDRDWGSTMKPITAYAPAIESGVYTS
                     TAQSTNDSVYYWPGTTTQLFNWDLRYNGWMTIQAAIMLSRNVPAVRALEAAGLDYARS
                     FLSSLGINYPEMHYSNAISSNNSSSDKKYGASSEKMAAAYAAFANGGIYHKPRYVNKV
                     EFSDGTSKTFDEKGKRAMKETTAYMMTDMLKTVLTYGTGTAAAIPGVAQAGKTGTSNY
                     TDEELAKIGEKYGLYPDYVGTLAPDENFVGFTKRYAMAVWTGYKNRLTPVYGSSLEIA
                     SDVYRSMMTYLTNGYSEDWTMPNGLYRSGGFLYLSGTYASNTDYTNSVYNNLYSNNTT
                     TASSQTTSDDTSSSNDTSNSTNTDNNGSHPSTDDKKTTH"
     gene            complement(2295..2894)
                     /gene="recU"
                     /locus_tag="ACGEUI_000776"
     CDS             complement(2295..2894)
                     /gene="recU"
                     /locus_tag="ACGEUI_000776"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFF8165629.1"
                     /translation="MVNYPHNLIRQKVSSVQKQNKVDFANRGMSFEAAINATNDYYLS
                     RQIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYCGVYKGHYVDFEAKETR
                     QKTAMPMKNFHLHQIEHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQIDNGS
                     KSMPIDYIRKNGFKVAFGAFPQVPYLNIIEQNFLGGDYN"
     gene            2975..3490
                     /locus_tag="ACGEUI_000777"
     CDS             2975..3490
                     /locus_tag="ACGEUI_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFF8165630.1"
                     /translation="MTAILITGYRSFEIGIFDHKDPRVSIIKQAIRKDLIGYLENGVD
                     WFIFTGNLGFEQWALEVANELKEEYPLQIATIFLFETHGDRWNEKNKEVLSQFRAVDF
                     VKYYFPNYEQPTQFSQYYQFLLEKTEGAYVFYDTENETNLKYFLKKAKDMPHYQLLLL
                     TFDRLNDMSQS"
     gene            3612..3938
                     /gene="gpsB"
                     /locus_tag="ACGEUI_000778"
     CDS             3612..3938
                     /gene="gpsB"
                     /locus_tag="ACGEUI_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFF8165631.1"
                     /translation="MTSIIYSPKDIFEQEFKTSMRGFDKKEVDEFLDNVIKDYENFNA
                     QIEALKAENEALKKAKFQARNTVSATVQQPVPQPTRVAQSATNFDILKRISKLEKEVF
                     GKQIIE"
     gene            3952..4320
                     /gene="rnpB"
                     /locus_tag="ACGEUI_000779"
     ncRNA           3952..4320
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACGEUI_000779"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4383..5537
                     /locus_tag="ACGEUI_000780"
     CDS             4383..5537
                     /locus_tag="ACGEUI_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFF8165632.1"
                     /translation="MKETFRLVATAAAGLEAVVGKEVRALGFDCQVENGKVYFEGDVE
                     AIVKTNLWLRAADRIKIIVGQFPARTFEELFQGVFALDWENYLPLGAKFPISKAKCVK
                     SKLHNEPSVQAITKKAVVKKLQKHFHRPEGVPLQEVGSTFNIEVSILKDQATIMIDTT
                     GSSLFKRGYRVQKGGAPIKENMAAAILALSNWFPDKPLVDPTCGSGTFCIEAAMIGMN
                     IAPGFNRSFAFEEWSWVDKDMVQQVRDDAEQEANYEIELDISGFDIDGRMIEIAKSNA
                     EEAGLSDVITFKQMRLQDFRTDKVNGVVISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDELFELKYGQKADKKRKLYNGTLKVDLYQFYGERVKRHLI"
     gene            5551..7020
                     /locus_tag="ACGEUI_000781"
     CDS             5551..7020
                     /locus_tag="ACGEUI_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFF8165633.1"
                     /translation="MSEESKEVEVTKESQTLGLNEAKSMTIGEAVRKQSEIKAGVTKD
                     DSILDKYIKQHRDEVSSQKFDAKYTELDTASLDNFIKKQREALSKAGLVDDEPVSAES
                     AEQDSTLVEEVAEDLAPMETTAVVTGIPVEATVPVLDLDPSERVIPEPQMTKEEPKRD
                     QFLSEDSHHPAKQNTKKGWLIALFLLLLAILAVVFGWNHFLRQDSGKTTQTASKQTKT
                     SLQTDSAKKATRLKAAAKAFEKLYGTFYTDATKSKLKNSAFATLPDLEAALKALEGSA
                     YYDKAKAKVDSLKKAIAAITAVNGKFVSDVVVDGEKVSAEVKADANFDDLSSATLTTG
                     NANLDAVLQASITEGRQQLASKAEAAKAANEQAVQDQAAQGQSTSVAPSGYGLTSYDP
                     ASLQRHLSRVPYNQDVIADRANPSWAFNPGVLEKIVATSQARGYISGNQYILEPVNII
                     NGNGYYNMFKPDGTYLFSINCKTGYFVGNGKGYADALDY"
     gene            complement(7165..7647)
                     /locus_tag="ACGEUI_000782"
     CDS             complement(7165..7647)
                     /locus_tag="ACGEUI_000782"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFF8165634.1"
                     /translation="MTKEVIVESFELDHTIVKAPYVRLISEEFGPKGDRITNFDVRLV
                     QPNQNSIETAGLHTIEHLLAKLIRQRIDGMIDCSPFGCRTGFHLIMWGKHSSTDIAKV
                     IKSSLEEIATGITWEDVPGTTLESCGNYKDHSLFAAKEWAQLIIDQGISDDPFSRHVI
                     "
     gene            complement(7721..9052)
                     /locus_tag="ACGEUI_000783"
     CDS             complement(7721..9052)
                     /locus_tag="ACGEUI_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFF8165635.1"
                     /translation="MEIIGSLGVLVGVIVIIYLYVKEVNIIIAAPLATSLVILFNQMD
                     PTTTLLGKEPNQFMGALSTYILNYFAIFLLGSILAKLMETSGATTSIADYILKKVGHD
                     SPYKVLVAIFLISAILTYGGISLFVVMFAVLPLARSLFKKMDLAWNLIQVPLWLGIAT
                     FTMTILPGTPAIQNVIPIQYLDTSLTAAAIPSIVGSIGCVAFGLFYMKYCLAKSMARG
                     ETYATYAFDNEIQVKTKNLPHFLASILPLLLLIIIALTGSLFGNDFFKKNIIFIALLA
                     VILTASWLFRQFIPNKIAVFNLGASSSIAPIFATASAVAFGAVVMIVPGFTFFSDLIL
                     NIPGNPLISLAVLTSSMSAITGSSSGALGIVMPNFAQYYLDQGLNPEMIHRVATIASN
                     IFTIVPQSGVFLTFLALTGLNHKNAFKETFITVSVSTFIAQVIVIAFDLFS"
     gene            complement(9123..9902)
                     /locus_tag="ACGEUI_000784"
     CDS             complement(9123..9902)
                     /locus_tag="ACGEUI_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HFF8165636.1"
                     /translation="MTHTKEVAFITGAASGIGKQIGETLLKEGKTVVFSDINQEKLDQ
                     VVADYTKEGYDAFSVVCDVTKEEAINAAIDTVVEKYGRIDILVNNAGLQHVAMIEDFP
                     TEKFEFMIKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGVIGFAGKSAYNSAKHG
                     LIGLTKVTALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGIPLENVLEEVLYPL
                     VPQKRLIDVQEIADYVSFLASDKAKGVTGQACILDGGYTAQ"
     gene            complement(9935..10594)
                     /locus_tag="ACGEUI_000785"
     CDS             complement(9935..10594)
                     /locus_tag="ACGEUI_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HFF8165637.1"
                     /translation="MGTSTTVLSKEEIQTRIAKRVAQELEHNTLVNLGIGLPTKVANY
                     IPEGVTITLQSENGFVGLTGLTDDHYDPTIVNAGGQPVSIAPGGAFFDSSTSFGIIRG
                     GHVAATVLGALQVDKEASIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKAK
                     ILDKCTLPLTAQNVVNLIITEMGVFEYQDEGLCALEINPDYTFEDVQNVTEVTLIDKT
                     N"
     gene            complement(10596..11246)
                     /locus_tag="ACGEUI_000786"
     CDS             complement(10596..11246)
                     /locus_tag="ACGEUI_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HFF8165638.1"
                     /translation="MCKEITIKEAVAHVKDGDTIMVGGFMTNGTPEKLIDALVEKGVK
                     DLTLICNDAGFPDKGVGKMVANKQFSTIIASHIGLNREAGRQMTEGETVIDLVPQGTL
                     AERIRSGGFGLGGFLTPTGIGTEVAKGKEVITIDGKDYLLEKPLKADVALIFANKADK
                     NGNLQYAGSENNFNHVMAANAKTTIVEAREIVDVGQMDPNFVHTPGIFVNYLVKGA"
     gene            complement(11270..12457)
                     /locus_tag="ACGEUI_000787"
     CDS             complement(11270..12457)
                     /locus_tag="ACGEUI_000787"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFF8165639.1"
                     /translation="MKNIVIVEALRTPIGAFGGSFKSVSAVELGTTVLKKILDKTQVK
                     PEMVDEVILGNVLHAGLGQNVARQVAVHSGIPEDKTAFTLDMVCGSGLKAIQLAAQSI
                     MLGDADIVIAGGVENMSQATYVSTQHRFGQRLGNSQLIDTLVHDGLTDAFNNYHMGIT
                     AENVAQKYGISREEQDQFALESQEKAAKALENHRFADEIVPVSVPQRRKDPLIVTTDE
                     YPKVDTSLEKLQQLRPAFLPKEGTVTAGNASGINDGAALLMLMTEEKALELGLTPLVT
                     IESYASAGVAPELMGTGPIPATQKALKKAGLTISDLDLVESNEAFAAQSLAVLKDLKL
                     NPEIVNVNGGAIALGHPIGASGARILVTLIHEMKKRQVTRGLATLCIGGGQGTAVIVK
                     NNT"
     gene            12659..13555
                     /locus_tag="ACGEUI_000788"
     CDS             12659..13555
                     /locus_tag="ACGEUI_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8165640.1"
                     /translation="MDIKQIRYFIAIVENHFNLSQAAELLYVSQPTLSMMINDFEKRE
                     NVKLFKRKRGRIIGLTYLGDNYYKDAQKVLSLYDDMFLKLHDHSKGLKGSINIGIPPL
                     ILSVVFSEVMPKLILENPGIQFNVKEIGAYQLKNELLVGNVDVAVLLSPTGIADNLVE
                     TYEIQRSELSVCLSPRHRLASKKVIQWEDLTDEQLALFDPSFTVHHLVLEACERHQVR
                     PNIILTSSSWDFMLNSTKINHNVLTICPKPITELYQLKDIKCIPMERPISWRVVLTRL
                     RKKSYSEIEAYIMDDLLQSFFK"
     gene            13685..15292
                     /locus_tag="ACGEUI_000789"
     CDS             13685..15292
                     /locus_tag="ACGEUI_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFF8165641.1"
                     /translation="MVNIILLIVSALIGLILGYALISIRLKSAKEAAELTLLNAEQEA
                     VDIRGKAEVDAEHIKKTAKRESKANRKELLLEAKEEARKYREEIEQEFKSERQELKQL
                     ETRLAERSLTLDRKDENLSSKEKVLDSKEQSLTDKSKHIDERQLQVEKLEEEKKAELE
                     KVAAMTIAEAREVILMETENKLTHEIATRIRDAERDIKDRTVKTAKDLLAQAMQRLAG
                     EYVTEQTITSVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVILSGFDPI
                     RREIARMTLESLIADGRIHPARIEELVEKNRLEMDNRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSFGQNVLRHSVEVGKLAGILAGELGENVALARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPVVVNTIASHHGDVEPDSVIAVLVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIATSFDGVQNSFALQAGREIRIMVQPEKISDDQVVILSHKVRE
                     KIENNLDYPGNIKVTVIREMRAVDYAK"
     gene            15402..16037
                     /gene="gmk"
                     /locus_tag="ACGEUI_000790"
     CDS             15402..16037
                     /gene="gmk"
                     /locus_tag="ACGEUI_000790"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFF8165642.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEELIKTGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFVFLTPPDLDELEDRLVGRGTDSQEVIAQRIERAKEEIALM
                     REYDYAVVNDEVALAAERVKRIIETEHFRVERVIGRYDKMIKITKNSFKAK"
     gene            16053..16370
                     /gene="rpoZ"
                     /locus_tag="ACGEUI_000791"
     CDS             16053..16370
                     /gene="rpoZ"
                     /locus_tag="ACGEUI_000791"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFF8165643.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGATPTQEFKSV
                     KSTLQALEEIESGNVVIHPDPSAKREAVRAKIEAERLAKEEEERKIKEQIAKEKEEEG
                     EKI"
     gene            16435..18819
                     /locus_tag="ACGEUI_000792"
     CDS             16435..18819
                     /locus_tag="ACGEUI_000792"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFF8165644.1"
                     /translation="MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGK
                     GNRLLQGFIIGFGQEDSSSLKLIQTVLDPEPVLNQEQLTLADQLRKTVFSYKITLLKA
                     MIPNLLNSNYDKVLRPESGLKKSDRDFLFEGKPSVLYSTLDREKEKIALKGIQAGHIT
                     VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQA
                     FSRDVVAYFVTNHLIRIDERPIDRSESYFDQIKPSSFLTLNEQQASAVTEIVEQIGKP
                     SKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQMTSRFISRFGKQVA
                     IMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESN
                     PRYHAREVALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEV
                     TIVDFRDYIGQQAVSNFTPYLIDKIKERLVKKEQVVLMLNRRGYSSFVMCRDCGYVDK
                     CPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFDELQGVI
                     PEAKILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNAD
                     TSLNLPDFRASEKTFQLLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKKQDFEAFY
                     RYEMSIRHQMAYPPYYFTVGITLSHRLEASVVKKAYQVTELLKSHLSDNIKILGPTPK
                     PIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL"
     gene            18881..19816
                     /gene="fmt"
                     /locus_tag="ACGEUI_000793"
     CDS             18881..19816
                     /gene="fmt"
                     /locus_tag="ACGEUI_000793"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFF8165645.1"
                     /translation="MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKD
                     IKVTPVKQLALEHGISIYQPEKLSGSQELIEIMGLGADGIITAAFGQFLPTILLDSVS
                     FAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
                     NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEHEKLDWTMSN
                     QEVFNHIRGMNPWPVAHTFLEGQRLKIYEAQLAEGEGLPGQVVVKTKKSLVIATGQGA
                     LSLIVVQPAGKPKMSIIDFLNGIGRKLEVGDIIGR"
     gene            19806..21128
                     /gene="rsmB"
                     /locus_tag="ACGEUI_000794"
     CDS             19806..21128
                     /gene="rsmB"
                     /locus_tag="ACGEUI_000794"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFF8165646.1"
                     /translation="MADNWKKSTRGKALLVIEAIFDQGAYTNIALNQQLSNKALSAKD
                     RALLTEIVYGTVSRKISLEWYLAHYVKDRDKLDKWVYYLLMLSLYQLTYLDKLPAHAI
                     VNDAVGIAKNRGNKKGAEKFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVK
                     KLEDQFGSDRSVAIMESLFVRSKASIRVTDPLKLEEVAEALDAERSLLSATGLTKASG
                     HFAASDYFTNGDITIQDESSQLVAPTLNIDGDDIILDACSAPGGKTSHIASYLKTGKV
                     IALDLYDHKLELVKENANRLGVADNIETRKLDAREVHRHFEKDSFDKILVDAPCSGIG
                     LIRRKPDIKYNKESQDFNALQAIQLEILSSVCQTLRKGGIITYSTCTIFDEENRQVIE
                     AFLQSHPNFEQVKLNHTQADIVKDGYLIITPEQYQTDGFFIGQVRRVL"
     gene            21166..21906
                     /locus_tag="ACGEUI_000795"
     CDS             21166..21906
                     /locus_tag="ACGEUI_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFF8165647.1"
                     /translation="MKISLKTDIGQKRSNNQDFINKFDNKKGITLVILADGMGGHRAG
                     NIASEMTVTDLGREWVKTDFTELSQIRDWLFETIQSENQRIYDLGQSEDFKGMGTTVE
                     AVALVESSAIYAHIGDSRIGLVHDGHYTLLTSDHSLVNELVKAGQITEEEAASHPQRN
                     IITQSIGQASPVEPDLGVRVLEPGDYLVINSDGLTNMISNDEIVTILGSKVSLDEKNQ
                     EMIDLANLRGGLDNITIALVHNESEDVE"
     gene            21903..23801
                     /gene="pknB"
                     /locus_tag="ACGEUI_000796"
     CDS             21903..23801
                     /gene="pknB"
                     /locus_tag="ACGEUI_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFF8165648.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVL
                     RTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIGEEDGQQFLVMEYVDGADLKRYI
                     QNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSIIEENHNVPQALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYN
                     RSRERKIIFENVESTKPLPKVASGPTASVKLSPPTPTVLTQESRLDQTNQTDALQPPT
                     KKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQELYDV
                     GLKVGKIRQIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKGLDY
                     QEAMNSLIETYGVPKSKIKIERIVTNEYPENTVISQSPSAGDKFNPNGKSKITLSVAV
                     SDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSSKAIVSG
                     QSPYYGTSLSLSDKGEISLYLYPEETHSSSSSSSSTSSSNSSSINDSTAPGSNTELSP
                     SETTSQTP"
     gene            23924..24616
                     /gene="liaF"
                     /locus_tag="ACGEUI_000797"
     CDS             23924..24616
                     /gene="liaF"
                     /locus_tag="ACGEUI_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFF8165649.1"
                     /translation="MKKFQFFLLIECILLAMGIMTILDNDLSSFILILVLILLALRFY
                     NQDSRNNFLLTVSLLFLFLIFMLNPYIIMAVLLGIVYIFINHFSQVKKKNRFALIRFK
                     EEKIEVNNTKHQWIGTANYESDYYCFDDINIIRISGNDTVDLTNVIVTGMDNIIVIRK
                     IFGNTTILVPIDVTVTLDVSSIYGSVDFFRCQQYDLRNESIKLKETDNQSLKKVKIIV
                     TTIAGNVEVDRR"
     gene            24613..25617
                     /locus_tag="ACGEUI_000798"
     CDS             24613..25617
                     /locus_tag="ACGEUI_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8165650.1"
                     /translation="MKKRYYALVWLYSTITILSIVFVVMDNLGITFNYLRNHLWQVER
                     LGFSILLLIVSVTLLLLLLWIIMDDNSKRNINQNLKYILNNRRLYLDETSEINTNLSR
                     LSKKMSHLTANMQKKESAYILDSQEVVKQERKRIARDLHDTVSQELFASSLILSGISM
                     SLEQLDKTQLQTQLTTVEAMLQNAQNDLRILLLHLRPTELANRTLSEGLHMILKELTD
                     KSDIEVIYKETIAQLPKTMEDNLFRIAQEFISNTLKHAKASRIEVYLNQTSTELQLKM
                     IDDGVGFDMDQVRDLSYGLKNIEDRVNDLAGNLHLISQKGKGVSMDIRLPIVKGDDDE
                     "
     gene            25610..26251
                     /locus_tag="ACGEUI_000799"
     CDS             25610..26251
                     /locus_tag="ACGEUI_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8165651.1"
                     /translation="MSKIKVILVDDHEMVRMGLKSFLNLQADIDVVGEASNGREGVDL
                     ALALKPDVLVMDLVMPELGGVEATLEVLKKWKEAKVLVLTSYLDNEKIYPVIDAGAKG
                     YMLKTSSAAEILNAIRKVSKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLA
                     KGYDNQTIADELFISLKTVKTHVSNILAKLEVGDRTQAVVYAFRHHLVPQDDN"
     gene            26288..27688
                     /locus_tag="ACGEUI_000800"
     CDS             26288..27688
                     /locus_tag="ACGEUI_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HFF8165652.1"
                     /translation="MDAKLKYKAKKIKMVFFDIDDTLRVKDTGYMPESIQRVFKALKA
                     KGILVGIASGRARYGVPQEVQDLHADYCVKLNGAYVKDDAKTIIFQAPIPADVVVAYK
                     KWADDMGIFYGMAGRHEAVLSARNDMISNAIDNVYAQLEVCPDYNEYHDVYQMWTFED
                     KGDGLQLPAELAEHLRLVRWHDNSSDVVLKGTSKALGVSKVVDHLGLKPENILVFGDE
                     LNDLELFDYAGISIAMGVSHPLLQEKADFITKKVEEDGILYALEELGLIDKELQFPQL
                     DLPNHKGPKATIKTNHGDMTLVLFPDHAPKTVANFLGLAKEGYYDGIIFHRIIPEFMI
                     QGGDPTGTGMCGQSIYGESFEDEFSDELYNLRGALSMANAGPNTNGSQFFIVQNSKIP
                     YAKKELERGGWPAPIAASYAANGGTPHLDRRHTVFGQLVDETSFQVLDLIAGVETGAQ
                     DKPKEDVIIETIEVFD"
     gene            27688..28065
                     /locus_tag="ACGEUI_000801"
     CDS             27688..28065
                     /locus_tag="ACGEUI_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HFF8165653.1"
                     /translation="MKIGDKLHGTITGIKPYGAFVALENGTTGLIHISEIKTGFIDDI
                     DQLLAIGNQVLVQVIDIDEYSKKPSLSMRTLAEEKQHFFHRHRYSNSRHKIGFRPLEE
                     QLPQWIEESLQFLKKNTKKSLEP"
     gene            complement(28083..29024)
                     /gene="cysK"
                     /locus_tag="ACGEUI_000802"
     CDS             complement(28083..29024)
                     /gene="cysK"
                     /locus_tag="ACGEUI_000802"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFF8165654.1"
                     /translation="MTKIYKTITELVGQTPIIKLNRLIPNEAADVYVKLEAFNPGSSV
                     KDRIALSMIEAAEAEGLISPGDVIIEPTSGNTGIGLAWVGAAKGYRVIIVMPETMSLE
                     RRQIIQAYGAELVLTPGAEGMKGAIAKAETLAIELGAWMPMQFNNPANPSIHEKTTAQ
                     EILEAFKEISLDAFVSGVGTGGTLSGVSHVLKKANPETVIYAVEAEESAVLSGQEPGP
                     HKIQGISAGFIPNTLDTKAYDQIIRVKSKDALETARLTGAKEGFLVGISSGAALYAAI
                     EVAKQLGKGKHVLTILPDNGERYLSTELYDVPVIKTK"
     gene            complement(29152..29784)
                     /locus_tag="ACGEUI_000803"
     CDS             complement(29152..29784)
                     /locus_tag="ACGEUI_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFF8165655.1"
                     /translation="MEHFKTIKASGFFEESIKKSRFICHIKRVSTEEDGKNFVNAIKK
                     EHYKANHSCFAMIIGNNRQIKRSSDDGEPSGTAGIPILSVLEKQCLTNVVVVVTRYFG
                     GIKLGTGGLIRAYSNMTATAIKRFGIIEVKQQIGLEITLSYPQYQLYSNLLDQLALTE
                     TETKFSDTIKTTLYCDTERVENLIDTLTNYYHGQISCEKIGSKVIEFPCD"
     gene            29839..31164
                     /locus_tag="ACGEUI_000804"
     CDS             29839..31164
                     /locus_tag="ACGEUI_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8165656.1"
                     /translation="MESIENSYGRLLLESQLPDSAKQLAQPLKSVVILRGKMICQRCH
                     YQLDEEARLPSGAYYCRFCLVFGRNQSDKLLYAIPPMHFPKGNYLVWGGQLTAYQEMI
                     SQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
                     LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQL
                     HYAASQALKEGGAKILLTATSTRTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFN
                     LFKMIHRQKLPLKILKYLKKQRKTGYPLLIFLPTIIMAESVTAILKELLPAEQIACVS
                     SQSQNRKEDITAFRQGKKTILITTSILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAG
                     RVGRSIDRPDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN"
     gene            31136..31801
                     /locus_tag="ACGEUI_000805"
     CDS             31136..31801
                     /locus_tag="ACGEUI_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFF8165657.1"
                     /translation="MICLLCQQISQTPISITEIIFLRRISSPICQQCQKSFQKIGKSV
                     CATCCANSDIIACRDCLKWENKGYNVNHRSLYCYNAAMKAYFSQYKFQGDYLLRKVFA
                     VELADVITKYYKGYIPVPVPVSPGCFRERQFNQVSAILEAANVSYLSLFEKLDNTHQS
                     SRTKKERLLVEKSYRLLKVSNIPDKILIVDDIYTTGSTIIALRKQLAKVANSDIKSLS
                     IAR"
     gene            31881..32429
                     /gene="raiA"
                     /locus_tag="ACGEUI_000806"
     CDS             31881..32429
                     /gene="raiA"
                     /locus_tag="ACGEUI_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFF8165658.1"
                     /translation="MIKFSIRGENIEVTEAIRDYVESKLTKIEKYFAKDQEIDARVNL
                     KVYRERSSKVEVTIPLDSVTLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKHRE
                     KVPTGQVFTTEFEAEEVDEIPEVQVVRTKNVTLKPMDVEEARLQMELLGHDFFIYTDS
                     EDGATNILYRREDGNLGLIEAK"
BASE COUNT        10505 a         5793 c         6649 g         9773 t
ORIGIN      
        1 gtcatttgtg tcatataaaa agcgagtcct cacagaacgt ggactcgctt tttatttaga
       61 ttatcactta aggtagatat taaaagtaac taaatggata tgtaataaaa ggtaggtaca
      121 gacaataagt agaggttaga gattaatgag ttgtcttttt atcatcggta gatggatgac
      181 tgccattgtt gtctgtattg gttgaattac ttgtatcatt gctactacta gtatcatctg
      241 aagtcgtttg gctagaagct gttgtcgtgt tattgctgta aagattgttg taaaccgaat
      301 tagtatagtc ggtgttgctc gcataggttc cgcttaagta gaggaatcca ccactgcgat
      361 aaagaccatt tggcatcgtc caatcttcac tgtaaccatt tgttaagtaa gtcatcatgc
      421 tacgataaac gtcagatgca atctctagac ttgatccgta tactggggtc aagcggtttt
      481 tgtaacctgt ccaaacagcc atggcgtagc gcttagtaaa gccaacaaag ttttcgtctg
      541 gcgctaatgt accaacataa tctggataaa ggccgtattt ttcaccaatt ttagctagtt
      601 cctcatcagt gtagttagaa gtccctgttt taccagcttg cgcaacacca ggaatggcag
      661 cagcagtacc tgtaccataa gtgagaacag tttttaacat atctgtcatc atataggccg
      721 tggtttcttt catggcacgt tttccttttt catcaaaagt tttacttgta ccatcactaa
      781 attccacttt attgacatac cttggtttat gataaatacc accatttgca aaagcagcgt
      841 atgcagcggc cattttttca ctacttgcac catatttttt atctgagcta ctgttattac
      901 ttgagatagc gtttgagtag tgcatttcgg gatagttaat acctaaactg cttaagaaag
      961 atcgagcata gtcaagtcct gcggcttcca gtgctcggac tgctgggaca tttcgcgata
     1021 gcataatagc agcttggatt gtcatccatc cgttatatct aaggtcccag ttaaacaatt
     1081 gggtagtggt tccaggccaa taatagactg agtcattagt cgactgagca gtagaagtat
     1141 aaacaccaga ttcaatagca ggagcatagg ctgtgattgg cttcatggta gaaccccagt
     1201 cacgatcagt taaaacagct tggttagtcc caaatgaaac attttcatct tgattacgtc
     1261 cgccaagctg agcaataaca tgaccatttg tcacatcaac aatcgttgaa gcaacttgga
     1321 aatcttggtc tggatagtaa acataatcac cagaatgata aatattataa agagtctgct
     1381 gcgcatcggg gataatattg gtataaactt ttaaaccagc ggtaaaaata tctttattcg
     1441 tttctttttt gacttcttca ataacttgtt ttagataatt atccatatat tttggataag
     1501 ttgagcgctg ttggagtgat tgtagacctt cagcgacggg agttgcgatg gcagtttcat
     1561 attctgcttt cgtcagatgt ttttccatgt acatctgttg caacacgacg ttacggcgat
     1621 tttgagcagc ttcaggatga aggtaaggat catattgact aggagcttga gggattccag
     1681 ccaatagggc tagttgggca taagataaat ccttaagatc cttgccataa taagacttag
     1741 cggctgtcag cataccatag ttgccattac ccatatatac tttgttgatg taaaaagtca
     1801 ggatttcttg tttagtatat tttcgctcca tttgaagagc aagccaaact tcttgagcct
     1861 tacgttttaa ggtttgatca gattcattag tagaaaaata ggctagttta atgagttgct
     1921 gatcaagcgt tgacccccct tgagtggtct gactcgttag attatgaaag gcagcaccaa
     1981 aaatacgata aagatctact ccacgatggt taaagaaacg tttatcttca attgaggtaa
     2041 tagcattaac tagattaata gggatactat cagctgttac attttcacgc ttttcagaac
     2101 ccaaatcagc aatcagattg ttattaccgt cataaaccaa gctagagttt gttgatttta
     2161 actgggcttc tgacagtttc ggagcactgc taatgtagaa ggtaaacaaa agaccaccaa
     2221 taataataac aaggataata aggctaagaa ttgcacttaa tacatacttt agccacttaa
     2281 ggatttttgg atttttaatt gtaatcaccg cctaaaaaat tttgttcaat tatgtttaaa
     2341 taagggactt gtggaaaggc tccaaatgct accttaaagc cattttttct gatataatca
     2401 atgggcatcg atttactgcc attatctatc tgataaaatg aaatcaaggc ttgagcaggt
     2461 aggtagtaag tctccttcag tgtggaaaag tgaagaagga caaagcagat tcctttttga
     2521 tgtaaaacgc aagccatatg ttcaatctga tgtaaatgga aatttttcat gggcatagct
     2581 gttttttgcc tggtttcttt tgcttcaaaa tcaacataat gtcctttata aacgccgcaa
     2641 tagtcagtcg ttgaagcttg tctaaagtac gcttcaacga tttttgcccg actgcgttta
     2701 gggtaatcca ctttgacaat ttggacagga gtaggttttt tgtgaatgac agctatttga
     2761 cgagataaat aataatcatt tgtcgcgttg atagcagctt caaaggacat acctctattg
     2821 gcgaagtcca ccttattttg cttttgaacg gaacttactt tttgtcgaat aagattatga
     2881 ggataattaa ccattgttct cctaatatta tctagttaat gctaaaaatt taagcacttt
     2941 tattataaca tattttttga aaggataaca cctaatgact gcgattctaa ttacaggtta
     3001 ccgaagcttt gaaataggga tttttgacca taaagaccca cgagtttcaa ttattaaaca
     3061 ggctattcgt aaagatttaa taggttattt ggaaaatggt gtggattggt ttatctttac
     3121 agggaacttg ggttttgaac aatgggcttt ggaagtagct aatgagctaa aagaggagta
     3181 tcccttgcag atagctacta tattcttgtt tgaaacgcat ggagataggt ggaatgaaaa
     3241 aaataaagaa gttttaagtc agtttagggc tgttgacttt gtaaaatatt actttccaaa
     3301 ttacgaacaa ccgacgcaat ttagtcaata ttatcagttt ttattagaaa agacagaagg
     3361 ggcttatgtt ttttatgaca cagaaaacga gaccaactta aaatattttc tcaagaaagc
     3421 aaaagatatg ccgcattatc agttgctatt gctaaccttt gatcggctaa atgatatgtc
     3481 acaaagctag cattgtattt ttaggggtat tggtatttaa atacttgctt tttgaactat
     3541 ttttctgtat cattgataat aatactgaaa tataattttg gtaaaaagat tgaccgttcg
     3601 gagagtaaag aatgacaagt attatttata gtcctaaaga tatttttgag caagaattta
     3661 agactagtat gcgagggttt gataagaaag aagttgatga atttcttgat aatgtaatta
     3721 aagattatga aaatttcaat gctcaaattg aagctttaaa agctgagaat gaagccttaa
     3781 aaaaggcgaa gtttcaagct agaaatacag tttcagcgac tgttcaacaa ccagtaccgc
     3841 aaccgactcg tgttgctcaa tcagctacta attttgacat cttgaaacgt attagtaaat
     3901 tggaaaaaga ggtttttggt aaacaaatta ttgagtaatg aaaactgtgc aatttttgga
     3961 taatcgcgta gtattttaat actatgagga aagtccatgc tagcactggc tgtgatgcca
     4021 gtagtgattg tgctaggcga acacataagc ctagggatgt gcatacacat tacggcgaag
     4081 gaaatggcta agtcgtttga tatgccaaag tacttctgaa agtgccacag tgacgtagtt
     4141 tttatggaaa cgtaaaaaat ggaacgcggt aaacccctca agctagcaac ccaaactttg
     4201 gtaggggcat gggatagttg gaaacgaaca agctatcctg actgtcaaca gacggtagac
     4261 agatgattat cgaaggaaat aattcctagt tatttccgga acaaaacatg gcttatagaa
     4321 aattgcataa taggttaagc tagcatttgc tagcttttat ttgatttaaa agaaagagat
     4381 atatgaaaga aacatttaga ttagtcgcaa ctgcagccgc aggtttagaa gctgtcgttg
     4441 gtaaagaagt cagagcacta ggctttgatt gtcaggttga aaatgggaaa gtttattttg
     4501 aaggtgatgt agaagcgatt gttaaaacaa atctttggtt acgtgcagca gatcgtatca
     4561 aaattatcgt aggacaattt cctgctagaa cctttgaaga attattccaa ggtgtctttg
     4621 ccttggattg ggaaaattat ttaccgctag gagctaaatt tccaatctca aaagccaaat
     4681 gtgttaagtc taaattgcat aacgagccaa gtgttcaggc gattaccaaa aaggccgttg
     4741 ttaagaaact gcaaaagcat ttccacaggc cagaaggagt tcctcttcaa gaagttgggt
     4801 caacgtttaa cattgaggta tctattttaa aagaccaagc cactattatg attgacacga
     4861 caggatctag cctatttaaa cgagggtatc gcgttcaaaa aggtggagca ccgatcaagg
     4921 aaaatatggc agctgctatc ttggccttat ctaactggtt tccagataaa cctctggttg
     4981 atcccacctg tggatcggga acgttttgca tcgaggcagc catgattggg atgaatattg
     5041 ctccaggatt taaccgttcg tttgcttttg aagaatggag ctgggtagat aaagatatgg
     5101 tgcaacaagt tcgtgatgat gctgaacaag aagctaatta tgaaatcgaa ttagatattt
     5161 ctggctttga tatcgatggc cgcatgattg agattgctaa aagtaatgct gaagaagctg
     5221 gtttatctga tgtgattacc tttaagcaaa tgcgtctgca agactttcga acagacaagg
     5281 ttaatggtgt agtgatatca aatcctcctt atggagaacg gttgcttgat gacaaagcgg
     5341 ttgacatctt gtataatgaa atgggacaaa cctttgcacc attgaaaaca tggagcaaat
     5401 ttatccttac aagtgatgaa ctttttgaac taaaatacgg gcaaaaagct gataaaaaac
     5461 gtaaacttta taacggcacc ctaaaagttg atttgtacca attttatggg gaaagggtaa
     5521 agcgacatct gatatagaga ggtagtaagc gtgtcagaag aaagcaaaga agtcgaagta
     5581 acaaaagaat cgcaaacatt ggggcttaat gaagccaagt caatgacaat tggtgaagct
     5641 gtacgcaaac agtcagagat aaaagcaggt gttactaagg atgatagtat tttagataag
     5701 tacattaagc aacaccgtga cgaagtttct tctcaaaagt ttgatgctaa gtatacagag
     5761 cttgatacag ctagtttaga taactttatc aaaaaacaaa gagaagcatt gagtaaagcc
     5821 ggtcttgttg atgacgaacc tgtgtctgcg gagtctgctg agcaagatag cactttggtt
     5881 gaagaagtag ctgaagactt agctccaatg gagactactg cggtggtaac tgggattccg
     5941 gtggaagcga cagtccctgt gttagatcta gatccatcag agagggtcat tccagaacca
     6001 cagatgacca aggaagaacc aaaaagagat cagtttcttt ctgaagatag ccaccatcca
     6061 gccaaacaaa acacgaaaaa aggttggtta atagctttat ttctgctatt attggccatt
     6121 cttgcagttg tatttggttg gaaccatttc ttgagacaag acagtggtaa aacaactcaa
     6181 acagcaagta aacagacaaa gacctctttg cagaccgaca gtgctaaaaa agcaacgcgc
     6241 ttgaaggctg ctgctaaagc tttcgaaaaa ctatatggta ctttttatac agatgccaca
     6301 aagagcaaat taaaaaatag tgcctttgct actttgccag atttagaagc tgccttgaaa
     6361 gctttggaag gctctgctta ctatgataag gcgaaagcaa aagtcgattc ccttaagaaa
     6421 gctattgctg ctattacagc ggttaacggt aaatttgttt ccgatgttgt tgtagatggt
     6481 gaaaaggttt cggctgaagt caaggctgat gctaattttg atgatttatc aagtgcaacc
     6541 ttgacaacag gaaatgcaaa tcttgatgct gtcttgcagg caagcatcac agaaggtcgt
     6601 cagcaactag ccagcaaggc tgaggccgct aaagcagcta atgagcaagc agtccaggat
     6661 caagcagctc aaggacaaag tacatcagta gcaccatctg gctatggctt gacaagttat
     6721 gatcctgcaa gcttacaacg gcatttatca cgtgtacctt acaatcaaga tgttattgct
     6781 gacagagcta acccatcttg ggcatttaat ccaggagttc ttgaaaaaat tgtagcaacc
     6841 tcacaagcta gaggttatat ttcaggcaac caatatattt tagagccggt taatattatt
     6901 aatggcaatg gttactataa tatgtttaag cctgatggca cttatctgtt ctctattaat
     6961 tgtaagacgg gttattttgt aggaaatggc aaaggttatg ctgatgcttt agattactga
     7021 gaaaatcaaa aaaactgaga taaggcaact tgtctcagtt ttttgattaa taaagtatgt
     7081 tgtcttaaaa tctgcagata gacttgtttt ttgtaacaag tcaaataggt cagtaggatt
     7141 gttatgtttg acaacacact agtatcagat gacatggcga ctaaaaggat cgtctgaaat
     7201 cccttgatca ataatcaatt gagcccattc tttggcggca aagaggctat gatccttata
     7261 gttcccacag gattcaagag ttgttccagg aacatcttcc caagtaatcc cagttgcaat
     7321 ttcttctagg ctggatttaa tcaccttggc aatatcagta gaactgtgtt ttccccacat
     7381 gataaggtga aaacctgttc gacagccaaa aggagagcaa tcaatcatcc catcaatgcg
     7441 ttggcggatg agcttggcaa gtaagtgttc aatggtatgc aaaccggctg tttcaataga
     7501 attttggtta ggctgcacta ggcgaacatc aaaatttgta atacgatcgc ccttgggtcc
     7561 aaattcttca gaaataagac gaacataagg ggcctttaca atagtatgat ctagctcaaa
     7621 actttcgacg ataacttctt ttgtcatttc aatctccttt gttttacact ttatcataac
     7681 acaaatagtg agaaagtggc tacttgtgga ggagaaaagg ctaagaaaac agatcaaagg
     7741 ctatcacaat gacctgtgcg ataaaagtac ttacagaaac agtaataaag gtttctttaa
     7801 aagcattttt gtggttaagt cctgtcaatg ctaagaaggt gagaaagaca ccactttggg
     7861 gcacaatggt aaagatatta gaagcaatag tggcaacacg gtgaatcatt tcgggattaa
     7921 ggccttgatc taaataatac tgggcaaagt taggcatgac aattcctaga gcaccggaag
     7981 aagagccggt aatagctgac atagaggaag tcaacacggc taaactaata agaggattac
     8041 cgggaatatt caaaatgaga tctgagaaga atgtgaaacc tgggacaatc atcacaacgg
     8101 ctccgaaggc aactgcagat gctgtcgcaa aaataggtgc aatagagctg ctagctccta
     8161 aattaaagac agcaatctta ttaggaataa actgtctaaa gagccagctt gcggtgagaa
     8221 tgacagctaa aagtgctatg aaaataatgt tcttcttaaa aaaatcgttt ccgaaaaggc
     8281 taccggttag ggcgataata atgagcaaca ataaaggtaa gatactggca aggaaatgtg
     8341 ggagattttt tgttttcact tgtatctcat tgtcaaaagc ataagtggca taagtttcac
     8401 cccttgccat actttttgct agacaatatt tcatataaaa gagcccaaaa gccacgcagc
     8461 caattgagcc aacgatactc ggaatggcgg cagcagttag tgaggtgtcc aagtattgaa
     8521 taggaataac attttgaatg gcaggtgttc cgggtaaaat cgtcatggta aaagttgcaa
     8581 taccgagcca aagaggaact tgaattaagt tccaagccaa gtccattttt ttgaataggc
     8641 ttcgtgcaag cggcaagaca gcaaacatga caacaaagag gctgattccc ccataagtta
     8701 atatagcact gatcagaaaa atagcaacga gtactttata aggactatca tgaccaactt
     8761 tttttaaaat ataatctgca atagaagtgg ttgcaccgct tgtttccatt aatttggcta
     8821 agatagaacc taataaaaag atagcaaaat aattgagaat ataagtggag agtgccccca
     8881 taaattggtt gggttctttt ccaagtaagg ttgtggttgg atccatttga ttaaacaaaa
     8941 tgaccaagct ggtagcaaga ggcgcagcga tgataatgtt aacttctttg acatacaggt
     9001 aaataataac aataacacct accaagaccc ccagagaacc aataatttcc atagttttat
     9061 gacctcctta ttagaaaata aagagacaga tcacaagata cagcctcttt atctctccaa
     9121 atttattgag cagtgtagcc accgtctaag atacaggctt gacctgtaac accttttgcc
     9181 ttatcactgg caaggaaaga cacatagtct gcaatttctt gaacgtcaat gaggcgtttt
     9241 tgaggaacaa gtgggtatag cacttcttca agaacatttt caaggggaat acctcttgtt
     9301 ttggaaaggt cttcaaactg gccacgaacc agtggtgtgt caacatatcc aggacaaatg
     9361 gcattgaccg taatgcctga atcagcagct tctaaggcag ttactttggt cagaccgatc
     9421 aagccgtgct tagctgaatt gtaggcggat ttgccagcaa aaccaatgac accattgata
     9481 gaagccatat taataatacg accgtgtttt tgagctttca ttgtaggaaa agcacgttta
     9541 atggcaataa atggtgctgt caacatgatt ttaatcatga attcaaattt ttcagttgga
     9601 aaatcttcaa tcatggcaac atgttgaaga cctgcgttgt taaccaaaat atcaatacga
     9661 ccatattttt caacaaccgt atcaatagca gcattgatgg cttcttcttt ggtgacatcg
     9721 cacacaacac taaaagcgtc atagccttct ttagtatagt cagcaacaac ctgatccagt
     9781 ttttcttgat taatgtcgga aaaaacaacc gttttacctt cttttaataa ggtttccccg
     9841 atttgttttc caattccgct tgcagcacca gtgataaatg caacttcttt agtatgtgtc
     9901 atgatactat cctcgtcttt cttttggtta gtctttagtt tgttttatca attagggtaa
     9961 cttcggtaac gttttgcacg tcttcaaagg tgtaatctgg gttaatttca agggcgcata
    10021 gaccttcatc ttgatattca aagactccca tttcagtaat gattaggtta acaacatttt
    10081 gagcagtcag aggaagggtg catttgtcta aaatttttgc tttgccttta ttggtatgct
    10141 ccattgcaac aatgactttt ttagcaccta ccaataagtc catggcccct cccatcccag
    10201 gaaccatttt tccaggaatc aggtaattag caatgctagc ttctttatca acttgtaagg
    10261 cacctaagac ggttgcggcc acatggccgc ctcgaataat accaaaggaa gttgagctat
    10321 cgaagaaagc ccctccagga gcaatactaa caggttgccc accagcatta acaatagtag
    10381 gatcgtagtg atcatctgtt aatccagtga gaccgacaaa accattttcg gactgcaggg
    10441 taatggttac accttcagga atatagttgg ctacttttgt tggcaaacct atgcctaagt
    10501 tgaccagtgt attgtgttct aattcttgtg caacgcgctt agcaatacgt gtttgaatct
    10561 cttcttttga aagaacagta gttgatgttc ccatgttagg ctcctttcac taagtagtta
    10621 acaaaaatgc caggagtatg gacaaaatta ggatccattt gccccacgtc aacaatttct
    10681 cttgcttcaa caatggtggt tttcgcatta gcagccatca catgattaaa gttattttca
    10741 gaacctgcgt attgcaaatt gccattttta tctgccttgt tagcaaaaat aagtgcgaca
    10801 tcagctttca aaggtttttc aagaaggtag tctttgccgt caatagtaat gacttctttt
    10861 cctttggcaa cttcggttcc tattccagta ggtgttaaga aaccgcctag cccaaaaccg
    10921 ccactgcgga tgcgctcagc taatgttccc tgaggtacaa gatcaattac agtctcgccc
    10981 tcagtcattt gtcggcctgc ttcacggttg agaccgatat gtgatgcaat aatagttgaa
    11041 aactgtttat tggcaaccat cttaccaacg cctttgtcag gaaaaccggc atcgttacag
    11101 attaaagtga ggtctttgac acctttttcc acaagtgcat caattaattt ttctggtgtg
    11161 ccatttgtca tgaagccacc aaccataatg gtatcaccat ctttaacgtg agctaccgct
    11221 tcttttattg tgatttcttt acacattttt tctaccaact ttcacgagac taagtattgt
    11281 ttttcacgat aacagcagtt ccttgtcctc caccaatgca aagtgttgct agtcctctgg
    11341 ttacttggcg ttttttcatt tcgtggatta atgtgactag aatgcgagcg ccactggcac
    11401 caataggatg acctaaagcg atggctcctc cattaacatt cacgatttct gggtttaact
    11461 ttaaatcttt taaaaccgcc aaggactgtg ctgcaaatgc ttcattggac tccactaagt
    11521 ccaaatcaga aattgttaat ccagctttct taagggcttt ttgagtggca ggaattggtc
    11581 cagttcccat cagttcaggt gcaacacctg cactagcata gctttcaata gtgactaaag
    11641 gtgttaaccc taattcaaga gctttttctt cagtcattaa catcaataag gctgcaccat
    11701 cgtttattcc tgaggcattc ccagcagtaa cggttccttc tttagggagg aaagcaggac
    11761 gtaattgctg taatttttca aggcttgtat caacttttgg gtattcatct gttgtaacaa
    11821 ttaaagggtc tttgcgtcgt tgaggcacag agacagggac aatttcgtca gcaaaacgat
    11881 gattttctaa tgctttagca gctttttctt ggctttctaa agcaaattga tcttgctctt
    11941 ctcttgagat gccatatttc tgagcaacgt tttcagcagt aatacccatg tggtaattgt
    12001 taaaagcatc tgttaagcca tcatgaacaa gagtatcaat caattgacta ttaccaagtc
    12061 gctgtccaaa acggtgctga gttgatacat aggttgcttg tgacatgttt tcgacaccac
    12121 cagcaataac gatatcagcg tcaccaagca tgatgctttg cgcagctaat tggattgctt
    12181 taagtccaga accgcaaacc atatctaatg tgaaagctgt tttatcttca ggaattcctg
    12241 agtggactgc aacttgtctt gccacatttt gtcctaggcc tgcatgaagg acgtttccta
    12301 ggatgacttc atcaaccatt tcaggcttta cttgcgtttt atcaaggatt tttttcagta
    12361 cagttgttcc aagttcaaca gcagagacac ttttaaagga gcctccaaaa gctccgatag
    12421 gagttcgtaa agcttcaacg ataactatgt ttttcataaa ataaacatcc tttcagtttt
    12481 agaaaagcgc tttccatgtc tctaagataa caagaaaacg ctaaaaataa aaatatagaa
    12541 tttttataag catataaatt tttttgatag agttggcaat agcatttaac atctacttgt
    12601 gtcataatat aggtaattat accggaaaaa gcaattgata agacaaggag aatcctattt
    12661 ggatattaaa caaattcgtt attttatcgc aatcgttgaa aatcatttta acttgagcca
    12721 ggcggcggaa ctgttgtatg tgtcgcagcc gacacttagc atgatgatca atgattttga
    12781 aaaaagagaa aatgttaagc tcttcaaacg aaaaagaggt cgtattattg ggttaaccta
    12841 tcttggtgat aattattata aggacgctca aaaggttctc agtctctatg atgacatgtt
    12901 tttgaaattg catgatcaca gtaaagggtt aaagggaagt atcaatattg gtattcctcc
    12961 tcttatttta tcagttgttt tctcagaagt aatgccaaaa ctgattttag aaaatccggg
    13021 gattcaattc aatgttaagg agattggtgc ttaccaacta aaaaatgaac tactcgtggg
    13081 aaatgttgac gtcgcagtct tgttatcccc aacaggcatc gctgataatt tggttgagac
    13141 ttatgaaatc cagagatcag aattatctgt atgcctgtct ccacgccatc gtttagcttc
    13201 caaaaaagtt attcagtggg aagatttaac agatgaacaa cttgccctat ttgatcctag
    13261 tttcacggtt caccatttag tgcttgaagc ttgcgagcgt caccaagtca gacctaatat
    13321 tattttgacg tcctcttcct gggattttat gcttaattct accaagatta atcacaacgt
    13381 cttaactatt tgtcccaaac ctattacaga attataccaa ttaaaggaca ttaagtgtat
    13441 tccaatggaa cgtcccattt cttggcgtgt tgtcttaaca cgcctccgga aaaaaagtta
    13501 ttctgaaata gaagcctaca tcatggacga cttgctacag tcttttttta agtagggttc
    13561 tcaaccactt ggcccaaatg ttaatttttt taaaaatgaa ttggaattgt gtaaacgctc
    13621 aatttatgat aaactaagag atggctttct attaatgaaa tctagaatat attgaggtac
    13681 aaacatggtt aatattattt tattaattgt ttctgccctc attggtttaa tattaggtta
    13741 tgcacttatt tcgattagac tcaagtctgc gaaggaagct gcagagttga ctcttttaaa
    13801 cgctgaacaa gaagctgttg atattcgtgg caaagcagaa gtagatgctg aacacatcaa
    13861 aaaaacagct aaacgtgaaa gtaaagcaaa tcgtaaagaa ttacttttag aagcaaaaga
    13921 agaggcaaga aaatatcgtg aagagattga acaagaattt aagtctgaaa gacaagagct
    13981 taaacaactc gagacacgct tagcggagcg ctccttaact cttgaccgta aagatgaaaa
    14041 cctatcaagt aaagaaaagg tactagatag taaagaacaa agtctgaccg ataaatctaa
    14101 acacattgat gagcggcaac ttcaagtgga aaaacttgaa gaggagaaaa aagcagaact
    14161 ggaaaaagtt gctgcgatga cgattgcaga agcgcgtgaa gtgattttaa tggagacgga
    14221 aaacaaactg acccatgaaa ttgcgacgcg cattcgagat gccgaacgtg acatcaagga
    14281 ccgaacagtt aaaacagcca aggacttgtt agcgcaagcc atgcaacgcc ttgctggtga
    14341 gtatgtgact gaacaaacta ttaccagtgt ccatctccca gacgacaaca tgaagggccg
    14401 aattattgga cgtgaaggcc gtaatattcg tactttagag agcttgactg gcattgacgt
    14461 tattattgac gatactcctg aagttgttat cttatcagga tttgatccta ttcgacgtga
    14521 aattgctcgt atgaccttgg aatctctgat tgctgatggt cgcatccatc cagctcgtat
    14581 cgaggaattg gttgagaaaa atcgtcttga aatggataat cgtattcgtg agtacggtga
    14641 agctgcagcc tatgagattg gtgcaccaaa ccttcatcct gatttgatta aaatcatggg
    14701 acgcctgcaa ttccgtacct cgtttggtca aaatgtccta cgtcactctg ttgaggttgg
    14761 taagttagct ggtattttag ctggtgagtt aggtgaaaat gttgctcttg cccgccgtgc
    14821 tggtttcttg catgatatgg gtaaagctat tgaccgtgag gttgaaggca gtcacgttga
    14881 gattggaatg gaatttgcac gtaaatacaa agaacatcca gttgttgtca acactattgc
    14941 tagccaccac ggagatgtgg agccagattc tgtgatcgct gtgctagtag ctgcagcaga
    15001 cgctctcagt tcggctcgtc caggcgctcg taatgagtca atggagaatt acatcaagcg
    15061 tcttcgtgat ttagaagaaa tcgcgacaag ttttgatggg gtacaaaata gttttgctct
    15121 acaagctgga cgtgagattc gtattatggt tcaacctgaa aaaatttcag atgatcaggt
    15181 tgtcattttg tcgcataaag taagagaaaa aattgaaaac aatctagatt acccaggaaa
    15241 tattaaagta actgttattc gtgagatgag agcggttgat tatgccaagt agaaaaagag
    15301 gaattatcct ctttttcttt atgacttgcc tttgaaaatg ttcttgaaaa agactagcct
    15361 ttttgataaa ctattaaaaa gcaactaata aggagaatac aatgtctgaa cgcggtttgt
    15421 taatcgtctt ttctggccct tcaggtgtcg gaaaaggaac ggtaagacaa gaaatttttt
    15481 caacgccaga tcataaattt gaatattcgg tttcaatgac gactcgtccg caaagaccgg
    15541 gcgaggttga tggggttgat tatttcttcc gcacacgtga agaatttgaa gaactcatta
    15601 aaactggtca aatgctagag tatgctgaat atgttggtaa ttactatggc acccctttaa
    15661 cttatgttaa tgagactctt gataagggaa ttgatgtctt tttagaaatt gaagttcaag
    15721 gagctcttca ggttaagtca aaagtaccag atggtgtttt tgttttttta acaccaccag
    15781 atttagatga attagaagac cgcctggttg gtcgtggtac agatagtcaa gaagtcatag
    15841 ctcaacgtat tgaaagagca aaagaagaaa ttgcccttat gcgcgaatac gattatgcag
    15901 ttgttaatga tgaagttgcc ttggcagctg agcgggttaa gcgtatcatt gagacagaac
    15961 actttcgtgt ggagcgcgtg attggtcgtt atgataaaat gattaaaata acaaaaaatt
    16021 cctttaaagc taaatagaaa agagtaagca ttatgatgtt aaaaccttcc attgatacct
    16081 tattagataa agtaccatcc aagtattcac ttgtgatttt acaagccaaa cgtgcccatg
    16141 aactagaagc tggtgccact ccaactcaag aatttaaatc agtgaagtca acacttcagg
    16201 cacttgaaga aattgaatca ggaaacgttg tgattcaccc agatccttca gctaaacgtg
    16261 aagctgtccg tgctaagatt gaggcagaac gcttggccaa agaggaagag gaacgtaaaa
    16321 tcaaagaaca aattgccaaa gaaaaagaag aagaaggaga aaaaatctaa ggtttcagaa
    16381 atcttagatt ttttcatatt tagtacgatg tccatgacag aaaaggagaa gcaaatggaa
    16441 aaagtagctc atgtgattgt agatattcca ttgatgcaaa cggacaagcc attttcttat
    16501 ggtattccaa aagagttagt ttctctcgtg cagcttggtt ctcgtgtgca tgttcctttt
    16561 ggaaaaggga atcgtttgtt gcaaggcttt attattggtt ttggccaaga agattcaagc
    16621 tctcttaaac tgattcagac cgttttagat cctgagccag tcctaaacca agagcaattg
    16681 acccttgctg atcagcttcg aaaaacagtt ttttcttata aaatcacgct cttaaaagca
    16741 atgattccta atcttcttaa ctctaactat gataaagtgc taaggccaga gtcaggtcta
    16801 aaaaagtctg atagggactt tctttttgaa ggaaaaccat ctgtcttata ttctacctta
    16861 gaccgcgaga aggaaaaaat agctcttaag ggcatacaag ctgggcatat tactgttagt
    16921 tatttagcca aagacaagaa aaacctaaag acagaaaaat attatcacgt cgacttagac
    16981 gccttagcgg tacatcccat ctctagtcgt gccaaaaagc gtcaattatt aaaagactat
    17041 ctgctaaccc atacgaagga agctaaactt gcaacgcttt atcaagcctt ttctagagat
    17101 gtggtggctt actttgtgac aaatcactta attcgtattg acgaacgtcc aattgatcgc
    17161 agtgagtcct attttgacca gatcaaacca tcttcttttt taaccttaaa tgaacagcag
    17221 gcgtcagctg ttactgaaat tgttgaacaa attgggaaac cgagtaaacc ttttttgatt
    17281 gaagggatta caggttcggg taagacagaa gtttatcttc atatcatcga agcggtttta
    17341 aaacaggata agacagccat tgttttagtg ccagaaattt cgttgactcc tcagatgacc
    17401 tctcgtttta tttcccgttt cggaaagcaa gtggctatta tgcattcagg cttatcagat
    17461 ggtgaaaagt ttgatgagtg gcgcaagata aaaacaggac aggctaaggt tgtggttggt
    17521 gcccgctctg ctattttctc cccactagag cggattggtg caattattat cgatgaggag
    17581 catgaatcaa cctataaaca agaaagcaac cctagatacc atgccagaga ggttgccctg
    17641 ctcagagcta aacaccatca agcagttgtg gttatgggct ctgctacgcc aagtattgaa
    17701 agccgagcaa gggctagtaa gggtgtctat cattttattc aattaacgca aagagcaaac
    17761 cctcttgcta agattccaga agtaacgatt gttgattttc gtgattatat tggacagcaa
    17821 gcagttagta attttacccc atatttgatt gataagatta aagaacgttt ggttaaaaaa
    17881 gaacaagttg tcttaatgtt aaatagacgt ggttattcta gttttgtgat gtgtcgggac
    17941 tgtggttatg tggacaaatg tcccaactgt gatatctctt taacccttca catggatact
    18001 aaaactatga attgccatta ctgtgggttt caaaaaccta tcccaattac ttgtcctgag
    18061 tgtcatagca atagtatccg ctattatggc actggaactc aaaaagcttt tgatgaattg
    18121 caaggggtta ttccagaagc taagatcttg agaatggatg ttgacaccac acgaaaaaaa
    18181 agaagtcaca aaaccatact agatagtttt ggtcgccagg aagcagatat tttgttaggg
    18241 acgcaaatga ttgccaaagg actggatttt ccaaatgtga ctttagttgg ggtactaaat
    18301 gcagatactt ccttaaattt gccggatttt cgagcgtcag aaaaaacgtt tcaattgtta
    18361 acacaggttg ctggtcgggc aggccgtgcc cataagccag gagaggtctt gattcaaact
    18421 tataatcccg atcactatgc tattcaatta gctaagaaac aagattttga agctttttac
    18481 cgttatgaga tgtctattcg tcatcaaatg gcttatcccc cttactactt tacagtgggt
    18541 attacgcttt cgcatcgttt agaagcatcg gttgtaaaaa aagcttatca agttacagaa
    18601 ctgctaaaga gtcacttatc ggataatatt aaaatattag gaccgactcc taagccaatt
    18661 gctagaacac acaatttata ccactatcaa attttgttaa aataccgttt tgaagataac
    18721 ctagaagaga cgctcaatcg tattcttgat tggagtcaag aagcagataa tcgtcattta
    18781 aaactaatta ttgattgtga accgcaacaa tttttataag gtaaatcact aaatctagga
    18841 tgatcagtta tgatataata aaaatacaaa gaggaaagtc atgataaaat tattatttat
    18901 gggaactccc caattttcag caactgtatt gaagggcttg ctagataatc ctgcctatga
    18961 gattttagga gtggttactc agcctgatcg agctgttgga cgcaaaaaag atattaaggt
    19021 aacacctgta aagcaattag ctcttgagca tggaatatca atctatcagc cggaaaaatt
    19081 atcaggctct caagaactca ttgagattat gggacttggt gcagatggta ttattacagc
    19141 agcatttggc caatttttac caacaatatt acttgattca gtttcatttg ctattaatgt
    19201 acatgcatca ttgttaccta aatatcgagg tggagcacct atccattatg ctattatgaa
    19261 tggagataaa gaggccggcg ttactattat ggaaatgatt aaagaaatgg atgcaggtga
    19321 catggtcgct aaagcaagca ctccaattct tgaaaccgat aatgtaggta ctctttttga
    19381 gaaattagcc atcattggtc gagacttact tttggatagt ttgccagctt atctttctgg
    19441 agaattaaaa cctattcctc aagatcacag ccaagctact ttttcaccta atatctctcc
    19501 agaacatgaa aaattagatt ggacaatgtc taatcaagaa gtgttcaatc atattcgagg
    19561 tatgaaccct tggccggtag cgcatacgtt tttagagggg caacgtttaa aaatttacga
    19621 agctcaacta gctgaaggag agggattacc tggacaagtt gttgtcaaaa ccaaaaaatc
    19681 tttggtaata gctactggac aaggtgcttt gtccttgatc gttgtgcaac cggctggtaa
    19741 acctaaaatg tcaataatag actttttaaa cggcattggt agaaaacttg aagtagggga
    19801 catcattggc agataattgg aaaaaaagta cgcgaggcaa agccctcctt gtcattgaag
    19861 ctatttttga tcagggggct tacacaaaca tagcgcttaa tcagcaacta agcaataaag
    19921 ctttatctgc taaggacaga gctctattaa ctgagattgt atatggtacc gtttcacgta
    19981 aaattagctt ggaatggtat ttagcccatt atgtcaaaga ccgagataaa ttggacaaat
    20041 gggtttatta tctccttatg ctcagtcttt atcaactgac ttatttagat aagttaccag
    20101 cgcatgccat tgttaacgat gcggttggta ttgccaaaaa tcgtggcaat aaaaaaggtg
    20161 cagagaagtt tgtcaatgct attttacgtc aattcaccag ccatcctctc ccagatatgg
    20221 aaacaattaa gcgtcgaaat aagtattatt cggtcaagta ttcactgcct gtttggttgg
    20281 ttaaaaagtt agaagaccaa tttggttctg acagatcagt ggccatcatg gaaagtttat
    20341 ttgtgcgtag taaggctagt attcgcgtga cagacccttt gaagctagag gaagttgctg
    20401 aggctttaga tgcagaacgt tcccttttat cagcaactgg tttaaccaaa gcgtcaggtc
    20461 attttgcagc cagtgattat ttcacaaatg gggacatcac tattcaagat gaaagcagtc
    20521 agcttgtagc tcctacttta aacattgacg gtgacgacat aattcttgat gcgtgtagcg
    20581 ctccaggggg caagacctct catatagcat cttacttaaa aacaggtaaa gttattgcct
    20641 tagatctcta tgatcataaa ctagagttgg tgaaggaaaa tgctaaccgc ttaggagttg
    20701 ctgataacat tgagacaaga aaactagatg ccagagaggt tcatcgtcat tttgaaaagg
    20761 atagctttga taagattttg gtggatgctc cttgctcagg aattggtttg attcgtcgaa
    20821 aacccgatat caaatataat aaagaaagtc aagatttcaa tgctctgcaa gctatccagc
    20881 tagagatatt gtctagtgtt tgtcaaacct tgcgtaaagg tggtataata acatacagta
    20941 cttgcactat ttttgatgag gagaatcgtc aagtcatcga agcattttta cagagtcatc
    21001 ctaattttga acaagtaaaa ctgaaccata cacaagcaga tattgtcaag gatggttatc
    21061 ttattatcac accagaacaa taccagactg atggcttctt tattggacaa gtcagacgag
    21121 tcttgtagta ttttaggagg aaactgactg agtcagaaaa aacgtatgaa gatttcatta
    21181 aaaacagaca ttgggcaaaa acgctcaaac aatcaagatt ttatcaataa gtttgataat
    21241 aaaaaaggca tcactttagt gattttagca gacggtatgg gtggccacag agctggaaat
    21301 attgccagtg agatgacagt cacagacctg ggaagagaat gggtaaaaac agacttcaca
    21361 gagttaagtc agattagaga ttggttattt gaaactatcc agtcagaaaa tcaacggatt
    21421 tatgatctag gccaatcaga agactttaaa ggaatgggaa cgactgttga agcagtagct
    21481 ttagttgaat ctagcgccat ttatgctcat attggtgatt cacggattgg tcttgtccat
    21541 gatgggcatt ataccttatt gaccagtgat cattctttag tgaatgagtt ggttaaggca
    21601 ggacagatca cagaagaaga agctgctagc catccacaaa gaaatattat cactcaatct
    21661 attggtcaag cttccccagt agagcctgat ttaggagtga gggttttgga gccaggggat
    21721 tatcttgtca ttaacagtga tggtctaacc aatatgattt caaatgatga aattgtgact
    21781 atcttgggca gtaaggttag tcttgatgaa aaaaaccaag aaatgattga cttagctaac
    21841 ctaagaggag gcctagataa tattacaatc gctttggttc ataacgaaag tgaggacgtc
    21901 gaatgattca gattggcaaa ttatttgctg gtcgttatcg cattctgaaa tctattggcc
    21961 gcggtggtat ggcggatgtt tatttagcaa atgacttgat cttggataat gaagacgttg
    22021 caatcaaggt cttgcgtacc aattatcaaa cagatcaggt agcagttgcg cgtttccaac
    22081 gagaagcgcg ggccatggct gaattgaacc atcccaatat tgttgccatc cgggatatag
    22141 gtgaagaaga cggacagcaa tttttagtaa tggaatatgt ggatggtgct gacctaaaga
    22201 gatacattca aaatcatgct ccattatcta ataatgaagt ggttagaatt atggaagaag
    22261 tcctttctgc tatgacttta gcccaccaaa aaggaattgt acacagagat ttaaaacctc
    22321 aaaatatcct actaactaag gagggtgttg tcaaagtaac tgatttcggc atcgcagtag
    22381 cctttgcaga aacaagcttg acacaaacta attcgatgtt aggcagtgtt cattacttgt
    22441 ctccagaaca ggctcgcggc tccaaagcga cgattcaaag tgatatttat gcgatgggga
    22501 ttatgctctt tgagatgttg acaggccata tcccttatga cggcgatagt gctgttacga
    22561 ttgccttgca acattttcaa aagcctcttc catctattat cgaggagaac cacaatgtgc
    22621 cacaagcttt ggagaatgtt gttattcgag caacagccaa gaaattaagt gatcgttacg
    22681 ggtcaacctt tgaaatgagt cgtgacttaa tgacggcgct tagttataat cgtagtcggg
    22741 agcgtaagat tatctttgag aatgttgaaa gtaccaaacc cctccccaaa gtggcctcag
    22801 gtcccaccgc ttctgtaaaa ttgtctcccc ctaccccaac agtgttaaca caggaaagtc
    22861 gattagatca aactaatcaa acagatgctt tacagccccc caccaaaaag aaaaaaagtg
    22921 gtcgtttttt aggtacttta ttcaaaattc ttttttcttt ctttattgta ggtgtagcac
    22981 tctttactta tcttatacta actaaaccaa cttctgtgaa agttcctaat gtagcaggca
    23041 ctagtcttaa agttgccaaa caagaactgt atgatgttgg gctaaaagtg ggtaaaatca
    23101 ggcaaattga gagtgatacg gttgctgagg gaaatgtagt tagaacagat cctaaagcag
    23161 gaacagctaa gaggcaaggc tcaagcatta cgctttatgt gtcaattgga aacaaaggtt
    23221 ttgacatgga aaactacaaa ggactagatt atcaagaagc tatgaatagt ttgatagaaa
    23281 cttatggtgt tccaaaatca aaaatcaaaa ttgagcgcat tgtaactaat gaatatcctg
    23341 aaaatacagt catcagtcaa tcgccaagtg cgggtgataa atttaatcca aacggaaagt
    23401 ctaaaattac gctcagtgtt gctgttagtg atacgatcac tatgcctatg gtaacagaat
    23461 atagttatgc agatgcagtc aataccttaa cagctttagg tatagatgca tctagaataa
    23521 aagcttatgt gccaagctct agctcagcaa cgggctttgt gccaattcat tctcctagtt
    23581 ctaaagctat tgtcagtggt caatctcctt actatggaac gtctttgagt ttgtctgata
    23641 aaggagagat tagtctttac ctttatccag aagaaacaca ctcttctagt agctcatcga
    23701 gttcaacgtc aagttcaaac agttcttcaa taaatgatag tactgcacca ggtagcaaca
    23761 ctgaattaag cccatcagaa actacttctc aaacacctta acaaagaata gttagtctca
    23821 tgttttactg gcatgccact aactattctt tttaactact cgtcatataa agatgcaatt
    23881 ttccaggaat tctgataaaa tacaaagaaa taaggggaaa ctaatgaaaa aatttcaatt
    23941 ctttttactt atcgagtgta tactgctagc tatgggtatc atgacaatct tggataatga
    24001 tttatcaagt tttatcctaa ttctagttct tatcttatta gcattacgct tttataacca
    24061 agatagccgc aataattttt tactaactgt cagtttattg tttctttttt taattttcat
    24121 gcttaatccc tacattatca tggctgttct tttggggata gtttacattt tcattaacca
    24181 tttttcacaa gtcaaaaaga agaatcgctt tgccttgatt cgtttcaagg aagaaaagat
    24241 tgaagtgaac aataccaagc atcaatggat tggtactgct aattatgaaa gtgattatta
    24301 ttgttttgat gacattaaca tcattcgaat ctcaggaaat gacacggttg acttaaccaa
    24361 tgttattgtg acaggaatgg ataatatcat tgtgatacgt aaaatttttg gaaatacgac
    24421 tatattggta cctattgatg tcactgttac tttagatgtt agttccatat acggaagcgt
    24481 tgattttttt agatgtcagc aatatgattt gcgcaacgaa tctattaagt taaaagaaac
    24541 ggataaccaa tcccttaaaa aagtcaaaat tatcgttaca actattgccg gaaatgttga
    24601 ggtggatagg cgatgaaaaa acgttactat gctcttgttt ggctctactc aaccattacc
    24661 attttatcta ttgtttttgt ggtcatggat aatttaggaa tcacttttaa ctaccttcgc
    24721 aatcatttat ggcaggtcga acgtctaggg ttttcgattt tattgttgat tgtttctgtg
    24781 actttattat tgttgttatt gtggattatt atggatgata acagtaagcg taacatcaac
    24841 caaaatctaa aatatattct caataatcga cgactctatc tagatgagac atcagaaatc
    24901 aatactaatt taagtcgact ttctaaaaaa atgtctcacc tgactgctaa catgcaaaag
    24961 aaagaaagtg cttatattct tgatagccaa gaagttgtaa aacaagagcg caaacgaatt
    25021 gcaagagatt tacatgacac agtcagtcaa gagctattcg cttcgtcatt gattttatca
    25081 ggaatttcga tgagtttgga acaactggac aaaacacaat tacaaacaca gttaacaacg
    25141 gttgaagcaa tgttgcaaaa tgctcaaaat gatctacgca ttctcctttt gcatcttaga
    25201 cctaccgagc tagctaatcg gactttatct gagggccttc atatgattct taaggaatta
    25261 acagataaaa gtgatattga agtcatttat aaggaaacca ttgctcagct tcctaaaaca
    25321 atggaagata atctttttag aattgcccaa gaattcatta gcaacacgtt aaaacatgct
    25381 aaagctagtc gaattgaagt ttatctcaat caaacctcaa cagaattaca attgaagatg
    25441 attgatgatg gtgtaggatt tgatatggat caggtaaggg atttgagtta tggtctgaag
    25501 aatattgaag accgtgtcaa tgatttagca ggaaacctac atttaattag tcaaaaaggc
    25561 aaaggagttt ccatggatat tagactgccg atagtgaagg gagatgacga tgagtaagat
    25621 aaaagtgata ttggtcgatg atcatgaaat ggtccgcatg ggactcaaga gttttttgaa
    25681 tttacaagct gatattgatg tcgttggtga ggcctctaat ggacgtgaag gggttgattt
    25741 ggcattggct ttgaagccag atgttttggt tatggatcta gtgatgccag agttaggcgg
    25801 tgttgaggca actttagaag tcctaaaaaa atggaaagag gctaaggtac ttgtgttaac
    25861 ttcctatcta gataatgaaa agatataccc tgtcattgat gcaggagcga agggttatat
    25921 gttaaaaaca tcgagtgcgg ctgaaatttt aaatgccatt cgcaaggttt caaagggaga
    25981 gttagctatt gaaacagaag ttgacaaaaa aattaaggcg catgatcaac accctgactt
    26041 gcatgaggaa ctaacagcgc gtgagtatga cattttacac cttttagcta aagggtatga
    26101 taatcagacc atcgctgatg aactctttat ttccttaaaa accgtcaaaa cacatgtgtc
    26161 caatattcta gccaagttag aagttggtga ccgaacccaa gcggttgtct atgcttttcg
    26221 acatcattta gtcccccaag acgataatta atgctataat atagggtata gcaaattgga
    26281 ggaaagtatg gacgcaaaac taaaatacaa agctaaaaaa atcaaaatgg tgttctttga
    26341 tattgatgat accttgaggg tcaaagatac gggttatatg cctgagtcga ttcaaagagt
    26401 atttaaggct ttgaaggcaa aaggcatctt ggttggtatt gcttcaggtc gcgctcggta
    26461 cggtgttcca caagaagttc aagatttaca tgctgactat tgtgtcaaat taaatggagc
    26521 ttatgtcaaa gacgatgcta aaactattat tttccaagct ccgattccag cagatgttgt
    26581 tgtggcctat aaaaaatggg cggatgatat gggcattttt tatggaatgg cgggtcgtca
    26641 tgaagcggtg ttgtccgctc gaaatgacat gattagcaat gctattgata atgtctacgc
    26701 tcaactagag gtctgtccgg attataatga gtatcatgat gtttaccaga tgtggacttt
    26761 cgaggacaag ggagatggct tgcagttgcc tgctgaatta gcagagcacc ttcgtttggt
    26821 cagatggcac gataactcat cagatgttgt cttgaaaggt acgtcaaaag cgctaggtgt
    26881 ttcaaaagta gttgaccatt taggattaaa acctgaaaat attttggttt ttggagatga
    26941 actcaatgac ctagagttat ttgactatgc tggtatcagt attgcaatgg gagtatcaca
    27001 tccattactg caagaaaaag cagattttat cacaaaaaaa gttgaagaag atggcatttt
    27061 atatgccttg gaggaattag gattgattga caaagaatta cagtttccac aattggattt
    27121 gccaaaccat aaaggaccaa aagcaaccat taaaacaaac catggcgata tgaccctggt
    27181 cttgttccca gatcatgctc ctaaaacagt agccaatttc ttaggtttgg ctaaagaagg
    27241 gtactatgat gggattatct tccaccgtat tattcctgaa tttatgattc aagggggcga
    27301 tccaacagga acaggaatgt gtggacaatc catctatggt gaaagttttg aagatgaatt
    27361 ttcggatgaa ctctataatc tccgtggggc cttgtcgatg gctaatgcgg gaccaaatac
    27421 taacggcagt cagttcttta ttgttcaaaa tagtaagatt ccttatgcca aaaaagaact
    27481 agaacgtggt ggttggccgg ctccaattgc agcttcttac gctgcaaatg gtggaactcc
    27541 tcaccttgat cgtcgtcata cggtctttgg tcagcttgtg gacgaaactt cattccaagt
    27601 tttggatttg attgctggcg ttgaaactgg cgcacaggat aaacctaaag aagacgttat
    27661 cattgaaaca atcgaggtat ttgactaatg aaaattggcg acaaactgca tggcaccatc
    27721 actgggatta aaccatacgg agcctttgtt gcccttgaaa atgggacaac aggtctcatt
    27781 catatttcag aaataaaaac gggatttatt gacgatattg atcaattatt ggctataggc
    27841 aaccaggttt tagtacaagt aattgatatt gatgaatata gtaaaaagcc aagtttatct
    27901 atgcggacct tagcagaaga aaaacagcat tttttccatc gtcatcgtta ttccaacagt
    27961 cggcacaaga ttggctttag gccacttgaa gagcagttac ctcagtggat tgaagaaagc
    28021 ctgcagtttt taaaaaagaa cactaaaaag agtctggaac cttagtagtt ccaaaccctt
    28081 ttttatttcg tcttaattac tggtacatca tagagttcag tcgataaata gcgttcgcca
    28141 ttatctggta aaatagttaa cacatgtttg ccttttccta actgtttagc gacttcaata
    28201 gcggcgtaaa gagcagctcc agaagaaatc ccaaccagga agccttcctt agctcctgtt
    28261 agtcgagcag tttctaaagc atctttcgat ttaacacgga taatttggtc ataggctttg
    28321 gtatctaacg tgtttgggat aaatccagct gatatacctt gaattttatg tggtccaggc
    28381 tcttgaccag ataagacagc agattcttca gcttcaacag catagataac agtttcaggg
    28441 ttagcttttt tcaagacatg tgaaacacca gaaagtgttc ctccagtacc aacaccagat
    28501 acgaatgcat ctaaagaaat ctccttaaaa gcttccaaaa tttcttgagc tgttgttttt
    28561 tcatggatgc ttggattggc agggttatta aattgcatag gcatccaagc acctagttct
    28621 attgctaaag tttcagcttt tgcaatagcc cctttcatac cttctgctcc aggtgttaag
    28681 acaagctctg caccataagc ctgaatgatt tgccgtcttt ccaagctcat agtttcgggc
    28741 ataacaataa tgactcgata ccctttagca gcacctaccc atgcaagacc aatacctgta
    28801 ttaccacttg ttggttcgat aataacgtca ccaggactta tcagaccttc agcttcagca
    28861 gcttcaatca tcgataaagc aatacgatct ttaacagaag atcctgggtt aaaagcttct
    28921 aattttacat aaacgtcagc agcttcgttt ggaattaaac ggttaagttt gataataggt
    28981 gtttgaccta ctaattctgt tatagttttg taaattttag tcatagttta gcctcttttc
    29041 ttgatacaaa ataaatacgc tgtgtattta agtataagga gagatattat taaagtaaaa
    29101 tacagattta ctatcactct gataataaag ccatatcact tttgttgatg attaatcaca
    29161 ggggaattca ataactttgg atccgatttt ctcacagctt atctgaccat gataataatt
    29221 tgttaaagta tcaatgagat tttccacacg ttcagtatca caataaagtg ttgtctttat
    29281 cgtgtctgaa aacttagttt ctgtttcggt taaagccaat tgatctagca agttagagta
    29341 tagttgatat tggggataag aaagtgtgat ttctagtcct atctgctgtt taacttcaat
    29401 gatgccaaat cgtttaatag ctgttgcggt catgttagag tatgctctga ttaacccacc
    29461 tgttcctaat ttaataccac caaagtaacg cgttaccaca actactacat ttgttaaaca
    29521 ctgcttttct aacacagaaa ggataggtat tccagcagtt cctgaaggtt caccatcatc
    29581 acttgagcgc ttgatctgtc tgttgttacc gataatcatc gcaaagcagg aatgattagc
    29641 tttgtaatgt tcttttttga tagcattaac aaaattctta ccatcttctt cagtgctcac
    29701 tcgtttgata tgacaaatga aacgagattt tttaatactt tcttcgaaaa aaccactggc
    29761 tttgattgtc ttaaaatgtt ccataaaaaa attgtacaag aaaagataaa aattagcaaa
    29821 aaaagcgaat aataaagtat ggagtctatt gaaaatagtt atggacggtt gttgctagaa
    29881 agccagttac cagattcagc aaagcagctt gctcaacctt taaaaagtgt agtaatattg
    29941 agaggaaaaa tgatttgtca acgttgtcac tatcagcttg atgaagaagc acgattgcca
    30001 agtggagcct attattgccg attttgtctt gtctttggac gaaatcaatc agataaattg
    30061 ctctatgcta tcccacctat gcattttcca aaaggtaatt atctggtttg gggcggtcaa
    30121 ctaactgctt atcaagagat gatttcccaa caattattaa tcaatatgca aaatcaaaaa
    30181 acaacattag ttcatgctgt aactggtgca ggaaaaacag aaatgattta tgctgcgatt
    30241 gaggcggtaa ttaacacagg tggctgggtt tgcattgcca gcccaagagt agatgtttgt
    30301 gtagaggtgg ctactcgttt atcccaagcc ttctcctgca gcatctgttt aatgcacgca
    30361 gagtctcttc cttatcaaag agctcctatt atagtagcca caacgcatca gctgctaaaa
    30421 tttcacaagg cttttgatct cttaattatt gatgaagtag acgcattccc ttttgtaaat
    30481 aatattcaac ttcattatgc tgcaagtcag gctttaaaag aaggtggcgc aaagatttta
    30541 ttaactgcaa cttcaactag aactttagaa agaaaggtaa acaagggaga agtagtcaag
    30601 ctgacattgg cgagacgctt tcataatagg ccattagtta ttccaaaatt tataagaagt
    30661 tttaatctat ttaaaatgat acatcggcaa aaacttcctc ttaaaatttt aaaatactta
    30721 aaaaaacaaa gaaaaacagg ctatcccctt ttaatttttc tacccactat tataatggca
    30781 gaatctgtta cagcaatctt aaaagagtta ttgcccgccg aacaaatagc ttgtgtttct
    30841 agtcaatcac aaaacaggaa agaagatatc acagcttttc gtcaagggaa aaaaacgatt
    30901 ttaattacaa catcaatttt agaaaggggt gtgacatttc cccaaattga tgtttttgtt
    30961 cttgggtctc atcatcgtgt ctatagttcc caaagtcttg ttcaaattgc tggacgagtt
    31021 ggaagatcta tagacagacc agacggaacg ttatatttct ttcatgaagg tatcagtaaa
    31081 gcaatgctac tagcaagaaa agagattaaa gaaatgaatt ataaagggta ttcgcatgat
    31141 ctgtctacta tgtcaacaaa ttagtcaaac accaataagt attacagaaa tcatcttttt
    31201 aagacgtatc tcttcaccga tttgtcaaca atgtcaaaaa agctttcaaa agataggaaa
    31261 aagtgtttgt gcgacatgtt gtgcaaactc agatataata gcttgtcgag attgtctaaa
    31321 atgggaaaac aaaggataca atgtaaatca tagaagctta tattgttata atgctgctat
    31381 gaaagcatac ttcagtcaat ataagtttca aggagactat ttattaagaa aagtttttgc
    31441 agtagaactt gccgatgtta tcaccaagta ctataaaggc tatatcccag tcccggttcc
    31501 tgtaagtccc ggttgttttc gagaaagaca atttaatcaa gtgagcgcta ttcttgaggc
    31561 agctaatgtt agctaccttt ctctttttga aaagctagat aatactcacc aatcttccag
    31621 aacaaaaaaa gagagattat tagtagaaaa atcttatcga ctactaaaag tatcaaacat
    31681 tcctgataaa atccttatag tagatgatat ttatactact ggtagtacaa ttatcgctct
    31741 tagaaaacaa ttggctaaag tagcaaatag tgacattaaa agtttgtcaa ttgcacgtta
    31801 aaacttgcaa attttcatga aagcagtata atagtattaa agataaagta agcgcttcaa
    31861 gcgcgagaaa gaggtatcct atgattaagt tcagtattcg tggagaaaat atcgaagtga
    31921 cagaagctat tcgcgattat gtagaatcaa agcttactaa aatcgaaaaa tattttgcta
    31981 aagaccaaga aatagatgca cgtgttaatt taaaagttta tcgggagaga tcttcaaaag
    32041 ttgaggtaac gatcccatta gattcagtaa cccttcgtgc tgaagatgtt tcacaggata
    32101 tgtacggctc tattgattta gttgttgata agatcgaacg ccaaattaga aaaaataaaa
    32161 ccaaaattgc taaaaaacat cgcgaaaagg ttccaactgg tcaagtcttt acaactgagt
    32221 ttgaagctga agaagtcgat gagataccag aagtacaagt tgtacgtact aaaaatgtga
    32281 cattaaaacc aatggatgtt gaagaagcgc gtttacagat ggaactattg ggacatgatt
    32341 tctttatcta cactgattct gaagatggcg caacaaacat tttataccgt cgcgaagatg
    32401 gaaacctagg attaatagaa gctaaatagt tcacaactaa aacctacagg gatgattata
    32461 cgtattttaa aagtgggaaa aagctattca tgtttagagg tgacatgatg aaaatgagat
    32521 taagtgatag atacgacagt cattatgcta acaaaagtat ctattacagt tcctctttaa
    32581 gtaaatctta cgtaaaatta gtttgaaaaa agattagtcc caattctggg ctagaaattc
    32641 tcaaaaaaat atgaaaaagc gcgttttcag aatataaagc gcgttttttt ctttttccat
    32701 ttaacaataa aaaactcatg
//
LOCUS       DAXWIQ010000017        31597 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000017 DAXWIQ010000000
VERSION     DAXWIQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31597
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.017"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            100..4206
                     /gene="cas9"
                     /locus_tag="ACGEUI_000807"
                     /gene_synonym="csn1"
     CDS             100..4206
                     /gene="cas9"
                     /locus_tag="ACGEUI_000807"
                     /gene_synonym="csn1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922251.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HFF8165659.1"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     gene            4206..5075
                     /gene="cas1"
                     /locus_tag="ACGEUI_000808"
     CDS             4206..5075
                     /gene="cas1"
                     /locus_tag="ACGEUI_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HFF8165660.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLIFKDAYKTELIHLSEIDILLLE
                     TTDIVLSTMLVKRLVDENVLVIFCDDKRLPTAMLMPFYGRHDSSLQLGKQMSWSETVK
                     SQVWTTIIAQKILNQSCYLGACSYFEKSQSIMDLYHGLENFDPSNREGHAARIYFNTL
                     FGNDFSRDLEHPINAGLDYGYTLLLSMFAREVVVSGCMTQFGLKHANQFNQFNFASDI
                     MEPFRPLVDKIVYENRNQPFPKIKRELFTLFSDTFSYNGKEMYLTNIISDYTKKVVKA
                     LNNEGKGVPEFRI"
     gene            5072..5413
                     /gene="cas2"
                     /locus_tag="ACGEUI_000809"
     CDS             5072..5413
                     /gene="cas2"
                     /locus_tag="ACGEUI_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HFF8165661.1"
                     /translation="MSYRYMRMILMFDMPTDTAEERKAYRKFRKFLLSEGFIMHQFSI
                     YSKLLLNNTANNAMIGRLREHNPNKGNITLLTVTEKQFARMIYLHGERNNCIANSDER
                     LVFLGEAFDES"
     gene            5403..6065
                     /gene="csn2"
                     /locus_tag="ACGEUI_000810"
     CDS             5403..6065
                     /gene="csn2"
                     /locus_tag="ACGEUI_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HFF8165662.1"
                     /translation="MNLNFSLLDEPIPLRGGTILVLEDVCVFSKIVQYCYQYEEDSEL
                     KFFDHKMKTIKESEIMLVTDILGFDVNSSTILKLIHADLESQFNEKPEVKSMIDKLVA
                     TITELIVFECLENELDLEYDEITILELIKSLGVKVETQSDTIFEKCLEILQIFKYLTK
                     KKLLIFVNSGAFLTKDEVASLQEYISLTNLTVLFLEPRELYDFPQYILDEDYFLITKN
                     MV"
     repeat_region   6168..6401
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6168..6203
                     /rpt_unit_seq="gttttagagctatgctgttttgaatggtcccaaaac"
     gene            6514..6921
                     /locus_tag="ACGEUI_000811"
                     /pseudo
     CDS             6514..6921
                     /locus_tag="ACGEUI_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
     gene            7045..8877
                     /gene="lepA"
                     /locus_tag="ACGEUI_000812"
     CDS             7045..8877
                     /gene="lepA"
                     /locus_tag="ACGEUI_000812"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFF8165663.1"
                     /translation="MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGIVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVAD
                     TRVGDTVTLANNPAKEALHGYKQMNPMVFAGIYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVHTTDEDMIE
                     VSNPSEFPDPTRVAFIEEPYVKAQIMVPQEFVGAVMELSQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYDMSEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVEKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDTKK"
     gene            9135..9971
                     /gene="slc2"
                     /locus_tag="ACGEUI_000813"
     CDS             9135..9971
                     /gene="slc2"
                     /locus_tag="ACGEUI_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored collagen-like adhesin Scl2/SclB"
                     /protein_id="HFF8165664.1"
                     /translation="MLTFGGASAVKAEENEKVREQEKLIQQLSEKLVEINDLQTLNGD
                     KESIQSLVDYLTRRGKLEEEWMEYLNSGIQRKLFVGPKGPAGEKGEQGPTGKQGERGE
                     TGPAGPRGDKGETGDKGAQGPVGPAGKDGQNGKDGLPGKDGKDGQNGKDGLPGKDGKD
                     GQDGKDGLPGKDGQPGKPAPKTPEVPQNPDTAPHTPKTPRIPGQSKDVTPAPQNPSNR
                     GLNKPQTQGGNQLAKTPAAHDTHRQLPATGETTNPFFTAAAVAIMTTAGVVAVAKRQE
                     NN"
     gene            10157..10594
                     /gene="msrB"
                     /locus_tag="ACGEUI_000814"
     CDS             10157..10594
                     /gene="msrB"
                     /locus_tag="ACGEUI_000814"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFF8165665.1"
                     /translation="METSDELKQRIGDLSYEVTQHAATESPFTGEYDNFFEKGIYVDI
                     VSGEVLFSSLDKFNSGCGWPAFSKPIENRMVTNHDDSSYGMRRVEVKSREAGSHLGHV
                     FSDGPKEAGGLRYCINSAALKFIPYDQMEKEGYAQWLTLFDET"
     gene            10709..11728
                     /locus_tag="ACGEUI_000815"
     CDS             10709..11728
                     /locus_tag="ACGEUI_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFF8165666.1"
                     /translation="MSTHLRKLPGLLLCLLLALPAWCLGRLFPIIGAPVFAILLGMLL
                     ALFYEHRDKTKEGISFTSKYILQTAVVLLGFGLNLTQVMAVGMQSLPIIISTIATALL
                     VAYGLQKWLRLDVNTATLVGVGSSICGGSAVAATAPVIKAKDDEVAKAISVIFLFNML
                     AALLFPSLGQLLGLSNEGFAIFAGTAVNDTSSVTATATAWDALHHSNTLDGATIVKLT
                     RTLAILPITLGLSLYRAKKEHDIVTEENFSLRKSFPRFILFFLLASLITTLMTSLGVS
                     ADSFHYLKTLSKFFIVMAMAAIGLNTNLVKLIKTGGQAILLGAICWVAITLVSLAMQL
                     SLGIW"
     gene            11935..12360
                     /locus_tag="ACGEUI_000816"
     CDS             11935..12360
                     /locus_tag="ACGEUI_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8165667.1"
                     /translation="MKRKFLIGSHGRLASGLQSSIDILAGMGQALETIDAYVDDSDYT
                     SQIDDFIAGVAADEQGLIFTDLLGGSVNQKMVTAVMNSGKDNIFLITNSNLATLLSLV
                     FLKPGEALTKDEIVTVINESQVQLVDLVPETNSEDDFFD"
     gene            12379..12870
                     /locus_tag="ACGEUI_000817"
     CDS             12379..12870
                     /locus_tag="ACGEUI_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8165668.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEDSIKLFNDPRAKDKRIFVIVNSVKDACAIAKEVPDLEAVN
                     VANVGRFDKSDPASKVKVTPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQ
                     LVN"
     gene            12887..13696
                     /locus_tag="ACGEUI_000818"
     CDS             12887..13696
                     /locus_tag="ACGEUI_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8165669.1"
                     /translation="MLVPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGLLLGDM
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISHIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNAFVEHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDLELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            13693..14520
                     /locus_tag="ACGEUI_000819"
     CDS             13693..14520
                     /locus_tag="ACGEUI_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8165670.1"
                     /translation="MTSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASAGSAMGAVVFLLLYNIPAFIIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQDYLDQLFIGIVPLMVTLAAFWLLRKKVNINWIMFGIMFLGIILGLLGIC"
     gene            14656..16305
                     /locus_tag="ACGEUI_000820"
     CDS             14656..16305
                     /locus_tag="ACGEUI_000820"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8165671.1"
                     /translation="MRGEQVEEHFKKQLQDDISRHFSYQSLMLSLLLIGLFIIFSLAP
                     QQLGLYRDINATATRYHRLISKQEALLDDLGKNSLLPFLNKNLSTADLSKHYFHLRHS
                     SQTSPELLLFSPSQDLLFASNPHLGNVFSKSVYIQEVLRATHPPKTLFKVAMDSEDGH
                     YLMIIKPMIDQNQLKGYAFLVMSGKDFLHPTKTLTSELVIADKLDNTFTFSNREFIAS
                     SLDKINSQYLHHYFVFQDNRAFITRKVALQGGLWLYMYRPLIPMVSVMLFSLISSAVI
                     FVILQRKSSGLANRIAAKNSRAINQMVRDMSAISRQEKRRIDLESQDEFQYLSDQINQ
                     MVERLQQLHDKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSALTEKIV
                     IQLTKLLRYSLTDSSKPVLLKDDLSVIESYLVINQVRFEELQYSINLSPDLDSLEVPK
                     LFLLPLIENAIKYGLKERHDVKINIACYYQDDHIIFSVRDNGSGIDAHHQKVIREQLE
                     AGESHHGLINSYRRLKYHFSEVSLVFDQGDKQFNVSYHVKE"
     gene            16309..17097
                     /locus_tag="ACGEUI_000821"
     CDS             16309..17097
                     /locus_tag="ACGEUI_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8165672.1"
                     /translation="MYKLVIIEDEHIIRKWLRYAIDYKALDILVIGEAKDGKEGAVLI
                     KESQPDIVLTDINMPIMTAFDMFEVTKDQTYAKIILSGYADFPNARSAIHYGVLEFLT
                     KPIEKAALWECLQTIIAKIEKQKGSNQKTDACVYIPLPQMTDQIPEVVKDILEWVHAH
                     FQDKISTSRLAHDLGYSESYIYQNIKKHLQMPLSDYINQYRINQAIQLMQQEPDLMVY
                     EIAQAVGIYDYRYFDRVFKKYLGQTVKAFKEEHFMKDTDRSKPC"
     gene            17091..18137
                     /locus_tag="ACGEUI_000822"
     CDS             17091..18137
                     /locus_tag="ACGEUI_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8165673.1"
                     /translation="MLKLKRKWLLSFLLVIIILAIVNVAMYIFSSSKKDSAKELVILT
                     PNSQTILTGTIPAFEEKYGVKVRLIQGGTGQLIDQLGRKDKPLNADIFFGGNYTQFES
                     HKDLFESYVSPQVSTVISDYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQP
                     ALKGKIAFADPNSSSSAFSQLTNILLAKGGYTNADAWAYMKRLLVNMNSIRATSSSEV
                     YQSVAEGKMIVGLTYEDPCINLQKSGANVSIVYPKEGTVFVPSSVAIIKHAPNMTEAK
                     LFINFMLSRDVQNAFGQSTSNRPIRQDAQTSHDMKALETIATLKEDYAYVTKHKKKIV
                     ATYNQLRQRLEKAK"
     gene            18234..18739
                     /locus_tag="ACGEUI_000823"
                     /pseudo
     CDS             18234..18739
                     /locus_tag="ACGEUI_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048196.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            18916..19482
                     /locus_tag="ACGEUI_000824"
     CDS             18916..19482
                     /locus_tag="ACGEUI_000824"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFF8165674.1"
                     /translation="MTTEFDKMTRGEWYDANFDSELIQKRMMAQDLCFDLNQLKPSRE
                     EERSAVLNQLFGQSFEGLVLLSPFICDYGKNITFGKNCFINSNCYFMDGAKIALGDNV
                     FVGPSTGFYTANHPLDYKRRNEGLEKALPITIGDNVWFGANVNVMPGVTIGSGCVIAS
                     GSVVTHDIPVNSLAAGVPCQVVRKIEQE"
     gene            19498..20187
                     /locus_tag="ACGEUI_000825"
     CDS             19498..20187
                     /locus_tag="ACGEUI_000825"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFF8165675.1"
                     /translation="MHYNFLFFDLDHTLLDFDAAEEVALTKLLEEYQVIDIKAYKDYY
                     KPMNQNLWKQLEGGDISKADLVNSRFALLFAHFGVTVDGRQLAEGYQKHLKDQGQVYA
                     GAKELLADLTAQGYNLYAATNGIATIQQGRLQASGLAPYFKAIFISEQSGSQKPKKAF
                     YDWMTQQVSNYQPDQALMIGDSLSADVQGGINAGMDTLWYNPKHLLNNSPVHPTYEVS
                     DYQALLNCIAE"
     gene            20282..21679
                     /locus_tag="ACGEUI_000826"
     CDS             20282..21679
                     /locus_tag="ACGEUI_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFF8165676.1"
                     /translation="MAYQTIYPYTNEVLHTFDNMTDQGLADVLERAHLLYKKWRKEDH
                     LEERKAQLHQVANILRRDRDKYAEIMTKDMGKLFTEAQGEVNLCADIADYYADKADEF
                     LMSTPLETDSGQAYYLKQSTGVILAVEPWNFPYYQIMRVFAPNFIVGNPMVLKHASIC
                     PRSAQSFEELVLEAGAEAGSITNLFISYDQVSQVIADKRVVGVCLTGSERGGASIAEE
                     AGKNLKKTTLELGGDDAFIILDDADWDQLEKVLYFSRLYNAGQVCTSSKRFIVLDKDY
                     DRFKELLTKVFKTAKWGDPMDPETTLAPLSSAQAKADVLDQIKLALDHGAELVYGGEA
                     IDHPGHFVMPTIIAGLTKDNPIYYQEIFGPVGEIYKVSSEEEAIEVANDSNYGLGGTI
                     FSSNQEHAKAVAAKIETGMSFINSGWTSLPELPFGGIKHSGYGRELSELGFTSFVNEH
                     LIYIPNKTNNGNTKV"
     gene            21781..23577
                     /gene="uvrC"
                     /locus_tag="ACGEUI_000827"
     CDS             21781..23577
                     /gene="uvrC"
                     /locus_tag="ACGEUI_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFF8165677.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKEGTIIYVGKAKNLKKRVRSY
                     FRGSHDTKTELLVSEIVDFEYIVTESDTEALLLEINLIQKNMPKYNIKLKDDKSYPFL
                     KITNESFPRLVITRYIKKNDGLYFGPYPDSYTANEVKKLLDRIFPFKKCKNPINKVCF
                     YYHLGQCCAHTICHTDKAYWDRLIDDVKHFLNGKDDKIIEDLRSKMLAASKEMAFERA
                     AEYRDLISGIATMRTKQRVMSKDLQDRDIFGYYVDKGWMCVQVFFVRQGKLIQRDVNL
                     FPYYTDAEEDFLTYMGQFYQDKQHFIPKEVFIPEAIDEELVAAIVPTKIIKPKRGEKK
                     QLVALATKNARVSLQQKFDLLEKDIKKTSGAIENLGQLLRIDKPVRIEAFDNSNIQGT
                     SPVAAMVVFVDGKPSKKDYRKFKIKTVVGPDDYASMREVLFRRYSRVKKEGLQAPNLI
                     IVDGGVGQVNVAKDVIEKQLGLTIPVAGLQKNDKHQTHDLLFGNPLEVVPLPRRSEEF
                     FLLHRIQDEVHRFAVTFHRQVRRKNSFSSTLDHISGLGPKRKQLLLRHFKTITAIASA
                     TSEEIQALGIPKTVVEAIQQQITDNKNDRSSP"
     gene            23762..24364
                     /locus_tag="ACGEUI_000828"
     CDS             23762..24364
                     /locus_tag="ACGEUI_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFF8165678.1"
                     /translation="MKFLELNKKRHAIKTFNDQPVDYEDLRTAIEIATLAPSANNIQP
                     WKFVVVQEKKAELAKGLPLANKVQVEQAQYVVALFSDTDLALRSRKIARIGVKSLPDD
                     LIGYYMETLPPRFAAFNEVQTGEYLAINAGIVAMNLVLSLTDQKIASNIILGFDKSTT
                     NEILDIDPRFRPELLITVGYSDEKPEPSYRLPVDEVIERR"
     gene            24489..25898
                     /gene="pepV"
                     /locus_tag="ACGEUI_000829"
     CDS             24489..25898
                     /gene="pepV"
                     /locus_tag="ACGEUI_000829"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFF8165679.1"
                     /translation="MTTIDFKAEVDKRKKAMLADLVDLLRINSERDDQLADDKHPFGP
                     GPVKALEHFLAMAERDGYKTRNIDNYAGDFEFGQGDEVLGIFGHLDVVPAGSGWDTDP
                     YEPVIKDDRIYARGSSDDKGPTMACYYALKIIKELGLPVSKKVRFIVGTDEESGWGDM
                     DYYFAHNGLKNPDFGFSPDAEFPIINGEKGNITEYLHFAGDNKGAFVLHRFQGGLREN
                     MVPESATAVITAPHDLDVLEAALEQFLSEHGVKGSMKATDGKIEVTIIGKSAHGSTPE
                     AGVNGATLLAKFLNQFTFEGAAKDYLHVAGEVLHEDFAAEKLGLAYTDDRMGALSMNA
                     GVFTFDSQSADNTIALNFRYPKGTDAATLKAGLEKLPGLTKVSLSEHEHTPHYVPMDD
                     ELVATLLAVYEKQTGLKGYEQVIGGGTFGRLLERGVAFGAMFPGDENTMHQANEYMPL
                     ENIYRSAAIYAEAIYELIK"
     gene            complement(25966..27342)
                     /gene="mnmE"
                     /locus_tag="ACGEUI_000830"
     CDS             complement(25966..27342)
                     /gene="mnmE"
                     /locus_tag="ACGEUI_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002888396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFF8165680.1"
                     /translation="MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKN
                     LEQVASHTINYGHIINPKTGTIIDEVMVSVMLAPKTFTRENVVEINTHGGIAVTNEIL
                     QLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLS
                     QLINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLESLLRTAKRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQDRALLNLSQDSNRI
                     ILLNKTDLEQKIELEQLPDDYIPISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLS
                     NARHISLIEKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     misc_feature    27510..27645
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            27675..28175
                     /gene="rplJ"
                     /locus_tag="ACGEUI_000831"
     CDS             27675..28175
                     /gene="rplJ"
                     /locus_tag="ACGEUI_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFF8165681.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVIVDSRGLTVDQDTVLRR
                     SLRESGVEFKVIKNSILTRAAEKAGLDELKDVFVGPSAVAFSNEDVIAPAKVINDFTK
                     TADALEIKGGAIEGAVSSKEEIQALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAE
                     NKEGAA"
     gene            28240..28605
                     /gene="rplL"
                     /locus_tag="ACGEUI_000832"
     CDS             28240..28605
                     /gene="rplL"
                     /locus_tag="ACGEUI_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFF8165682.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGAEEAAKDSFDVELTSAGDKKVGVIKAVREITGLGLKEAKGLVDGAPANVKEGVAAA
                     EAEEIKAKLEEAGATITLK"
     gene            28904..29503
                     /locus_tag="ACGEUI_000833"
                     /pseudo
     CDS             28904..29503
                     /locus_tag="ACGEUI_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein"
     gene            29678..30003
                     /locus_tag="ACGEUI_000834"
                     /pseudo
     CDS             29678..30003
                     /locus_tag="ACGEUI_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670032.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            29994..31486
                     /locus_tag="ACGEUI_000835"
                     /pseudo
     CDS             29994..31486
                     /locus_tag="ACGEUI_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294852.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1250 family conjugative relaxase"
BASE COUNT        10037 a         5464 c         6644 g         9452 t
ORIGIN      
        1 aaagcatatt aaactaattt cggaggtcat taaaactatt attgaaatca tcaaactcat
       61 tatggattta atttaaactt tttattttag gaggcaaaaa tggataagaa atactcaata
      121 ggcttagata tcggcacaaa tagcgtcgga tgggcggtga tcactgatga ttataaggtt
      181 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt
      241 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca
      301 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca
      361 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg
      421 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct
      481 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat
      541 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat
      601 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag
      661 ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat
      721 gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct
      781 cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt
      841 ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca
      901 aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct
      961 gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga
     1021 gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa
     1081 catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat
     1141 aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct
     1201 agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag
     1261 gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac
     1321 ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa
     1381 gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga
     1441 attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg
     1501 aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca
     1561 gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta
     1621 ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc
     1681 aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc
     1741 attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat
     1801 tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt
     1861 aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg
     1921 gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa
     1981 gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg
     2041 atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt
     2101 aatggtatta gggataagca atctggcaaa acaatattag attttttgaa atcagatggt
     2161 tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac
     2221 attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta
     2281 gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg
     2341 gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat
     2401 cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt
     2461 atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa
     2521 aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa
     2581 ttagatatta atcgtttaag tgattatgat gtcgatcaca ttgttccaca aagtttcctt
     2641 aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg
     2701 gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta
     2761 aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt
     2821 ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc
     2881 actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat
     2941 aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga
     3001 aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg
     3061 tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag
     3121 tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa
     3181 gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa
     3241 acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg
     3301 gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg
     3361 tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agacaggcgg attctccaag
     3421 gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat
     3481 ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct
     3541 aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca
     3601 attatggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat
     3661 aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt tgagttagaa
     3721 aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct
     3781 ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt
     3841 agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat
     3901 gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta
     3961 gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa
     4021 aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt taaatatttt
     4081 gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga tgccactctt
     4141 atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt
     4201 gactgatggc tggttggcgt actgttgtgg taaataccca ctcgaaatta tcctataaga
     4261 ataatcatct gatttttaag gatgcctata aaacggagct gatccattta tcagaaattg
     4321 atattttgtt attagaaacg accgatattg tcttgtccac tatgctggta aaacggctag
     4381 tggatgagaa tgtccttgtc atattctgtg atgataaacg attaccaaca gctatgctga
     4441 tgccttttta tggtcgtcat gattcgagtt tacagcttgg gaaacaaatg tcctggtcag
     4501 aaacagtcaa atcgcaggtt tggacgacga ttattgctca aaagattttg aatcaatctt
     4561 gctatctagg agcatgctcc tattttgaaa aatcccaatc tattatggat ttatatcatg
     4621 gtttggaaaa ttttgatccg agtaatcgag aagggcatgc agcgagaatt tattttaata
     4681 cactttttgg gaacgatttc tcaagagatt tggagcatcc aatcaatgca ggtctggatt
     4741 atggttatac tttattattg agtatgtttg cgcgtgaagt ggttgtgtct ggatgtatga
     4801 ctcagtttgg gcttaaacac gctaatcagt ttaatcagtt caattttgct agcgatatta
     4861 tggaaccatt taggccttta gtggataaga ttgtttatga aaatcgaaat cagccttttc
     4921 ccaaaataaa gagagagtta tttactttgt tttcagatac attttcatat aatggtaaag
     4981 agatgtatct cacgaatatt attagcgatt atactaaaaa agttgtcaaa gctctgaata
     5041 atgaagggaa aggagttcct gaatttagga tatgagttat agatatatga gaatgatact
     5101 tatgtttgat atgccgacgg acaccgctga ggaacgaaaa gcctatcgaa aatttcggaa
     5161 atttttactt agtgaagggt ttatcatgca tcaattttct atttatagta agttgctgtt
     5221 gaataataca gctaacaatg ccatgattgg tcggctgagg gagcataatc ctaataaagg
     5281 aaatattaca ttactaacgg tcacggaaaa acagtttgca cgaatgattt atttacatgg
     5341 tgaaagaaat aattgtattg caaactccga tgaaagactt gtatttcttg gggaggcttt
     5401 tgatgaatct taatttttcc ttactagatg aaccgattcc attaagaggc ggtacaattc
     5461 ttgtgctcga agatgtctgt gtattttcaa aaatagtgca atattgttac caatatgagg
     5521 aagattctga acttaaattt tttgatcaca agatgaaaac aatcaaagaa tcagaaatca
     5581 tgcttgtaac agatatttta ggatttgatg ttaactcctc aaccatttta aaattgattc
     5641 atgcagattt agaatctcaa tttaatgaga aacccgaagt gaaatcgatg attgacaaat
     5701 tggttgctac gattacagaa ctgattgtct ttgaatgctt agaaaatgaa ttagatttag
     5761 agtatgatga aatcacaatc ctggaattga ttaagtcctt aggagtaaaa gtagaaacgc
     5821 aaagtgatac tatttttgaa aaatgtctag agatacttca aattttcaaa tatctcacta
     5881 agaaaaagtt gcttattttt gtcaatagcg gagcttttct aacaaaggat gaagtggcta
     5941 gtttacaaga gtatatatca ttgacaaatt taacagttct ctttttagaa ccacgtgaac
     6001 tatatgattt tccgcagtat attttagatg aagattattt cttaataact aaaaatatgg
     6061 tataatactc ttaataaatg cagtaataca ggggcttttc aagactgaag tctagctgag
     6121 acaaatagtg cgattacgaa attttttaga caaaaatagt ctacgaggtt ttagagctat
     6181 gctgttttga atggtcccaa aacgggtggt tggctgacgc atcgcaatat taagttttag
     6241 agctatgctg ttttgaatgg tcccaaaaca ggaatatccg caataattaa ttgcgctctg
     6301 ttttagagct atgctgtttt gaatggtccc aaaactaaat ttgtttagca ggtaaaccgt
     6361 gctttgtttt agagctatgc tgttttgaat ggtctccatt caacattgcc gatgataact
     6421 tgagaaagag ggttaatacc agcagtcgga taccttccta ttctttctgt taaagcgttt
     6481 tcatgttata ataggcaaaa gaagagtagt gtgatggaac aaacattttt tatgattaag
     6541 ccatatgggg ttaagcaagg ggaggtagtt ggagaggttt tacggtggat tgaacgccta
     6601 agatttacgt ttaagcgatt cgagccaaga caagctagtt cgaaatactt ggctaagcac
     6661 gacgaggcct tggtgataaa ccttttgatc ctaaacttaa agcttacatg acaagtggtc
     6721 ctgttttaat tgggataatt cttggggact aaggtggttt tgtctttttg aaccatgatg
     6781 agagtaacca atcctaagga tgctctttgc ggaaccattc gtgagaattt tgctcaggct
     6841 cctggtgatg atggaggtat ttttaacatg gtgcatgggt ctcattcaag agactctgct
     6901 aggcgtgaga tcgctctttg atctcatcaa agtaatcttg gtagctccgc tattctcttg
     6961 caagataatc cgtaagaaaa gataggcaaa ggcctacctt ttttgttata atagaaggaa
     7021 agactaaatg tataggaaaa tagaatgaac agtcaagatt taaaaaaacg tcaggagaag
     7081 attcgtaatt tctccattat cgcacatatt gaccatggca aatctactct cgctgaccgc
     7141 attttggaaa agacagagac ggtttcgtct cgtgaaatgc aggctcagtt attggactcc
     7201 atggatttgg agcgtgagcg tgggattacc atcaagttaa acgccattga gcttaattac
     7261 acggctaaag atggggaaac ctacattttc caccttattg ataccccagg gcatgtggac
     7321 tttacttatg aagtatcgcg ttcgttggca gcctgtgagg gagcgatttt agttgttgat
     7381 gcagcgcagg gaattgaagc gcagacattg gctaatgttt acctagccct tgataatgat
     7441 ttggaaattt taccggttat caataaaatt gatttgcctg ctgctgaccc tgagagagtc
     7501 cgtcatgaag tagaagatgt cattggactt gatgcttctg aggctgttct ggcatcagcc
     7561 aaggctggta ttgggatcga agagattctt gagcaaattg ttgagaaggt tcctgctcct
     7621 acgggtgatg tggatgcgcc tttacaggcc ttgatttttg actctgtgta tgatgcttac
     7681 cgaggggtaa ttttgcaggt tcggattgtg aacggtattg tcaagcctgg cgataaaatc
     7741 cagatgatgt ctaatggaaa aacctttgat gttacagaag ttgggatttt cactcctaag
     7801 gcagttggac gagatttcct tgcgacggga gatgttggtt atgttgcggc ttctatcaaa
     7861 acggtagcag atacccgtgt gggggatacg gtgactttag ctaataaccc tgctaaggaa
     7921 gccttgcatg gctacaagca gatgaacccg atggtctttg cagggattta tccgattgaa
     7981 tcaaataaat acaatgactt gcgtgaagca cttgaaaaat tacaactgaa cgatgccagt
     8041 ttacagtttg aacccgaaac gtcacaagcg cttggatttg gttttcgatg cggtttttta
     8101 ggcttgcttc acatggatgt gattcaagaa cgtttggaac gtgaatttaa cattgatttg
     8161 atcatgacag caccatctgt agtctaccat gttcacacaa cagatgagga catgattgaa
     8221 gtgtctaacc cttcagaatt tccagaccca acacgggtgg cttttattga agagccttat
     8281 gttaaagcgc aaatcatggt gccacaagag ttcgtagggg ctgttatgga attatcgcag
     8341 cgcaagcgtg gtgattttgt gaccatggat tacattgatg ataaccgtgt caatgtgatc
     8401 taccaaatcc cgctggctga aattgtgttt gatttctttg ataaattgaa atcctcaacg
     8461 cgaggctatg cgagttttga ttatgacatg tcagaatacc gcaggtcaca attggtgaaa
     8521 atggacattc ttttgaacgg tgataaggtc gatgcgctca gctttattgt tcataaagaa
     8581 tttgcttatg aacgcgggaa aattattgtt gagaaattga aaaaaatcat tccacgtcaa
     8641 cagtttgaag ttccgatcca agccgctatt ggtcaaaaaa ttgttgcgcg ttcagacatc
     8701 aaggcccttc gtaaaaacgt tttagcgaag tgttatggtg gtgacgtgtc tcgtaaacgt
     8761 aaattgctcg aaaaacaaaa ggctggtaaa aaacggatga aggctattgg ttctgtcgaa
     8821 gtccctcaag aagccttctt gagtgttctt tcaatggatg acgataccaa gaaataacca
     8881 agcctaatcg cttagtcttc agaggagaac tcttgtgggt ctacatgacc aaaaaatggt
     8941 tatcttatac aaaaagaact ttacaatcat tcgcacataa tgttataata atattataat
     9001 gttatctcat tattaggtgg gtctacattt aatattatta aagaaaaaag aggaaacaaa
     9061 aactgtgaac aaaacaaaac aaaacaaaac atagccttct ttgtcgctat ggcttaacct
     9121 cagcggctgc tcttttgctg acctttggag gtgcaagtgc ggttaaggcg gaagaaaatg
     9181 aaaaagtaag agagcaagaa aagctcatac agcaactttc tgaaaagcta gtggaaatta
     9241 atgacttaca aactttaaat ggtgataaag agagtataca gtctctcgta gattatctga
     9301 ctcgaagagg aaaacttgaa gaagaatgga tggaatattt gaattctggt attcaacgca
     9361 aactttttgt tggtccaaaa ggacctgcag gtgaaaaagg agaacaaggt cctactggaa
     9421 aacaaggcga gcgtggtgag accggccctg caggtccacg tggtgacaag ggcgaaactg
     9481 gtgacaaagg agcccagggt ccagtaggtc ccgctggcaa ggacggccaa aacggtaaag
     9541 atggtcttcc aggtaaagac ggcaaggacg gccaaaacgg taaagatggt cttccaggta
     9601 aagacggcaa ggatggccaa gatggcaaag atggtcttcc aggtaaagac ggtcaaccag
     9661 gtaaaccagc tcctaaaaca ccagaggtcc ctcaaaaccc agatactgca ccacatactc
     9721 caaaaacccc tcggatccct ggtcaatcaa aagacgtgac acctgctcct caaaaccctt
     9781 ctaatagagg tctaaacaaa ccacaaacac aaggtggtaa tcagctcgca aaaacaccgg
     9841 cagctcacga cacacacaga caattgccag caacaggcga aacaaccaat ccattcttta
     9901 cagcagctgc tgtagctatc atgacgacag ctggagttgt agctgttgca aaacgtcaag
     9961 aaaacaacta atcctctttt tgctacctaa ctgatggaaa gtaaaactca gagagaacct
    10021 caaggttctc tcttttttga atcaagatag tagctctagg tataacgtct tgtctgaggt
    10081 gctgtgaaca aatcagttac cgctcacctc tttttttggt aaaatgtaag cactaaaaca
    10141 tcaaaaggag ttttccatgg aaacatcaga tgagttaaaa caacgcattg gtgatctttc
    10201 ttacgaggtc acccagcatg cagcaacaga atctcccttt acaggagaat atgataattt
    10261 ttttgagaag ggtatctatg tcgatatcgt cagtggtgag gtcttgtttt catcactgga
    10321 taaatttaat tctggctgtg gttggcctgc cttttcaaaa cctattgaaa atcgtatggt
    10381 cacaaaccac gatgactctt cttatgggat gagacgagtt gaggttaaaa gccgtgaagc
    10441 aggatcgcat ttggggcatg tctttagtga cggccccaaa gaagctggag gcttgcgtta
    10501 ctgtatcaat tcagcagctc ttaaatttat tccttatgac cagatggaaa aagaaggcta
    10561 tgcccaatgg ctcacattgt ttgatgagac ataacagggt ttcaaaatga ctgaaattcc
    10621 cacaagcact ggattggtga ggcgtacggg tcagttttac aagcctttgt aaaaggtcta
    10681 taatagggaa tgaaaaaagg aggttagtat gtcaactcac ttaagaaaac ttccagggct
    10741 gttactttgc ttattgttag ctcttccagc ctggtgttta gggcgcttat ttcccattat
    10801 tggagcacct gtttttgcta ttcttttagg aatgttgtta gccttgtttt atgaacatcg
    10861 tgacaagact aaagagggaa ttagttttac atccaagtat attttacaaa cagcagtggt
    10921 tttgcttggt tttggattaa acctaaccca agttatggca gtgggcatgc agtctttacc
    10981 gattatcatt tcaactattg cgacagctct tttggtagct tatggcttac agaaatggct
    11041 gcgcttagat gtcaatacag ccaccttggt aggtgtagga tcttccattt gtggggggtc
    11101 tgctgttgca gcgacagctc ctgtcattaa ggcaaaggat gacgaggtcg ctaaggcaat
    11161 ttcagtcatt tttctcttta atatgttagc agctttgcta tttccaagtt taggacaatt
    11221 actaggctta tctaatgaag gttttgctat ttttgccggg acagctgtta acgacacttc
    11281 ttccgtgact gcaacggcca cggcctggga tgcccttcac cattccaata cactagatgg
    11341 agcaaccatt gttaaattga ctcgcacctt ggctattctc ccaattactt taggtttatc
    11401 cctttaccga gcgaaaaaag agcacgacat cgttacagaa gaaaacttta gccttaggaa
    11461 gtcattccct cgtttcatcc tcttcttttt attagcttct ctcatcacaa cattgatgac
    11521 cagtttggga gtttctgccg atagtttcca ttacctaaaa accttatcaa aattctttat
    11581 cgtgatggct atggcagcga ttggtttaaa cacaaacctg gttaaactga ttaagacggg
    11641 cggtcaggct atccttttag gagctatttg ctgggtagct atcacccttg tcagtttagc
    11701 catgcaatta agtttgggca tttggtaatc agacacgaga aatcagtttg actgatttct
    11761 ttttttgtat cttatttgtg tagaattgtc tacattttaa agagaataat gcatttttaa
    11821 aacgttttca tttgataaaa taaacctatc gttaaaccag ttcagtaagg acaactggtt
    11881 gagataagca attaccgttt actcagcggt ttttgttaat agaggaggat aggaatgaaa
    11941 cgaaaatttc tcattgggag tcacggcaga ctagccagtg gtttgcaaag ctctattgac
    12001 attttagcag gtatgggaca ggcacttgaa accattgatg cttatgttga tgatagtgac
    12061 tacactagcc agattgatga ctttatcgct ggggtagcag cagatgagca gggccttatt
    12121 tttactgatt tgttaggcgg cagtgtgaat cagaaaatgg taaccgctgt aatgaacagt
    12181 ggcaaagaca atatcttttt aatcacaaac agtaacttag ccactttatt gtcgttggtg
    12241 tttttaaaac caggcgaagc gcttactaaa gatgaaattg taaccgttat caatgaatcg
    12301 caagttcagt tagtggattt ggtccctgag acaaattcag aagatgactt ttttgattaa
    12361 tatctaggag gaagacaaat gattacccaa attcgtgttg acgaccgttt gattcacggg
    12421 caagtggcag ttgtatggac caaagagtta aatgcgccgc ttttagtagt ggctaatgat
    12481 gaagctgcaa aaaatgaaat cacacaaatg acgcttaaaa tggctgtgcc aaacggcatg
    12541 aaactgctta ttcgttcagt tgaagactcg attaagctct ttaatgatcc acgcgccaaa
    12601 gacaagcgta tctttgtgat tgtcaattct gttaaagatg cttgtgccat tgctaaagaa
    12661 gttccagact tagaagctgt taatgtggct aatgtggggc gctttgataa gtcagaccca
    12721 gcaagcaaag tcaaagtgac accaagtctt ttacttaacc cagaagaaat ggcagccgca
    12781 aaggaattag tcagtttacc agaacttgat gtctttaacc aagtcttacc atcaaatacc
    12841 aaggtccatt tgagccaatt agtcaactaa agagaggtaa aaaagtatgt tagtaccagc
    12901 aacaatggca gctttagctg tattgatttg ttttggggga aattatttaa ccggtcaaag
    12961 catgatggaa agacccctag tagttggact ggtcacaggt cttcttttag gagatatgaa
    13021 ggtcggtatc ttaatggggg cctctctaga agctcttttc ctaggaaatg tgaatattgg
    13081 gggtgttatt gcagctgagc ctgtaacagc gactgccatg gcaacaacct ttaccattat
    13141 ttcacatatt gaccaaaaag cagccatgac cctagctgtt ccaattggaa tgttggcagc
    13201 ctttgtggtt atgtttttga aaaacgtctt catgaatatc tttgctccaa tggttgataa
    13261 agctgcagca gctaaccatc aaggtaaact agtgatgctc cactatggca cttggattat
    13321 ctattactta atcattgcct ctatttcttt cattggtatc ttagtaggta gtggacctgt
    13381 taatgccttt gtggaacaca ttcctcaaaa tctcatgaat ggcttgagtg ctgcgggagg
    13441 ccttcttcca gctgtcggtt ttgccatgct gatgaaatta ttgtggacca acaaattagc
    13501 tgttttttac ctattgggct ttgtcttaac agcttatttg aaattaccag cggttgcagt
    13561 tgctgcccta ggagcagtta tttgcgtcat tagttcccaa cgtgatctag aattagacgc
    13621 aattactaga ggtgcaatta gtaaacaaac aacctttgac tctaaagaat cagaagagga
    13681 ggacttcttc gcatgacatc acaagataat ttgactaaag aagaccgtaa aatgcttcgc
    13741 tcagtctttt ggcgctcatg gacgatgaat gctagtcgta ctggggcaac acaataccac
    13801 gcagtagggg ttatttacac tttactacca gtcatcaatc gtttttataa gactgataag
    13861 gataaagcag aggcactggt ccgtcataca acatggttca atgccaccat gcacatcaat
    13921 aactttatca tgggtcttgt ggcatcgatg gaaaagaaaa acagtgaaga tcctgatttt
    13981 gatgctagtg ctattacggc ggtaaaagca tctctaatgg gacctatctc cggagttgga
    14041 gattccttct tctggggaat tttaagggtt attgcggcag gtatcgggat ttccttggca
    14101 agtgcaggct cagccatggg agctgttgtc ttcttactct tgtacaatat tcctgctttt
    14161 attattcact actatagcct ttatggtggt tattctgtag gtgcaggatt tatcaaaaaa
    14221 ctttacgaat ctggtggtat aaaaatcgtc actaaaacct caagtatgct tgggttaatg
    14281 atggtaggct caatgacggc ttcaaatgta aaatttaaaa cgattcttac agttgctgcg
    14341 aaaggtgcca aagaagcagc ttctattcaa gattaccttg accaattgtt tattggtatt
    14401 gtaccattaa tggtaacact agcagctttt tggcttctac gaaagaaagt gaatatcaac
    14461 tggattatgt ttggtatcat gttccttggc attatccttg gacttttagg tatttgctaa
    14521 taatctccta gcaataaagg taagtaagac aagttttctt gttttgctta cctttttcct
    14581 tttaagatga tgagacttgt tttgatgaaa aacgtagtag cattaagaaa tttgttataa
    14641 tgaaaggagc ttaagatgcg aggtgaacag gtggaagaac attttaaaaa acaattacaa
    14701 gatgatattt ctagacattt ttcgtaccaa tcgttaatgt tatcattgtt gctaattggt
    14761 ctttttatta ttttttcatt agcgccacaa caattaggcc tctatcgtga tatcaatgcc
    14821 actgcaaccc gttatcaccg tttgattagc aaacaagaag ccttgctgga tgacttaggg
    14881 aaaaatagct tactaccttt tttaaataaa aacctcagca ctgctgattt aagcaagcac
    14941 tattttcatt tgcgccatag cagccaaacg tcaccagaac ttttactatt ttcacctagt
    15001 caagaccttt tatttgctag taacccacat ttaggaaatg tttttagtaa atctgtttat
    15061 attcaagaag tcttgagagc aactcatcct ccaaaaacct tgtttaaagt tgctatggat
    15121 agtgaagatg gtcactactt gatgatcatt aagccaatga tagatcaaaa ccaactaaag
    15181 ggatatgctt ttttagtgat gagtggcaaa gattttcttc atcctacaaa aacattgacg
    15241 tcagagttgg tcattgctga taagctggac aatacgttta cgttttccaa tcgtgagttt
    15301 atagcatcta gtctagataa gatcaatagc cagtatttac accattattt tgtttttcaa
    15361 gataatcgag cctttatcac aagaaaggtt gccttacaag gaggtctttg gctttacatg
    15421 tataggccct tgattccaat ggtatcggtg atgttatttt cacttatttc atcagctgtt
    15481 atctttgtga ttttgcaacg taaatcaagt ggtttagcta accgaattgc agctaaaaat
    15541 tcaagagcaa tcaatcaaat ggttagagat atgagtgcga tctctcggca agaaaaaaga
    15601 cgtattgacc ttgagagtca agatgaattt caatatttat ctgatcaaat caatcaaatg
    15661 gtagagcgat tgcaacagtt acatgataaa acgttagatt tggaaactca aaaattatta
    15721 tttgaaaaac ggatgttaga agctcaattc aatccgcatt tcctctacaa tacgctagaa
    15781 accattttga ttacgagcca ttatgattct gccctaacag aaaaaattgt tatccaattg
    15841 acaaaattgt tgcggtatag tctcacggat tctagcaaac ctgtcttgct caaagatgat
    15901 ttaagtgtca tcgagtctta tttagtgatt aatcaggtgc ggtttgaaga attgcaatat
    15961 agcattaacc tatccccaga ccttgatagt cttgaagtgc ctaaattgtt cttgttacct
    16021 ttgatagaaa atgccatcaa atatggcttg aaagaacgac acgatgtcaa gattaacatt
    16081 gcttgttatt atcaagatga ccacattatt ttttctgtga gagataatgg ttcgggaatt
    16141 gatgctcatc accaaaaagt gattcgagag caattggaag ctggagagtc acaccatggg
    16201 ttaattaact cttatcgtcg gcttaagtat catttttcag aagtatcctt ggtttttgat
    16261 caaggtgata aacagtttaa tgttagttac catgttaagg agtagtctat gtacaaatta
    16321 gtcattattg aagatgaaca tattattagg aaatggttgc gatacgctat tgattataaa
    16381 gctttagata ttttggtcat tggcgaggct aaagatggca aagaaggagc tgttttaatc
    16441 aaagagagtc aaccagatat cgttttaacc gatattaaca tgcccatcat gacagctttt
    16501 gacatgtttg aagtaacaaa agatcaaact tacgccaaaa ttattttatc tggctatgct
    16561 gattttccaa atgcaaggtc tgccatacac tacggtgtac ttgaattttt aacaaaaccg
    16621 attgaaaaag cagctctttg ggaatgtctt caaaccatta ttgctaaaat agaaaagcaa
    16681 aaggggtcta atcaaaaaac agatgcttgc gtctatattc ccttacccca gatgactgat
    16741 cagattcctg aagtagtcaa agatattttg gagtgggtac atgctcattt tcaagacaag
    16801 atcagcacga gccgcctagc tcatgactta gggtatagtg agagttatat ctatcaaaat
    16861 atcaaaaagc atcttcagat gcctttaagt gattacatta atcaatatcg gattaatcag
    16921 gcaattcaat tgatgcaaca agaaccagat ttaatggtat atgaaatagc acaagctgta
    16981 ggtatttatg actatcgcta ttttgatagg gtttttaaaa agtatctagg acaaactgta
    17041 aaggctttta aagaagagca ttttatgaaa gataccgata ggagtaagcc atgttaaaac
    17101 tcaaacgcaa atggctatta agctttttgt tagtaataat tattctagca atcgttaacg
    17161 ttgcaatgta tatattctca tcgtcaaaaa aagatagtgc taaagaatta gttatcttga
    17221 ctcctaatag ccaaactatt ttaacaggga ctattccagc ctttgaggaa aagtatgggg
    17281 ttaaagtaag attaatccaa ggtgggacgg gccaacttat tgatcaatta ggtcgaaaag
    17341 ataaaccatt aaacgctgat attttctttg gtggcaatta cactcaattt gaaagccata
    17401 aagatttatt tgaatcttat gtttctccgc aggtttctac tgtcatttca gattaccaat
    17461 tgcctagtca tcgcgcaacc ccatatacga tcaatggcag tgtactgatt gttaataacg
    17521 aattagcaag aggacttcat attaccagtt atgaggattt gctacaacca gctttaaaag
    17581 gcaaaattgc ttttgctgat cccaacagtt catcaagtgc cttctcacag ctgactaata
    17641 tattgttagc taaggggggg tacacaaacg ctgacgcttg ggcttacatg aagcgcttgt
    17701 tggtcaatat gaattctatt agggctacga gttcttcaga agtctatcaa tctgtcgctg
    17761 agggtaagat gattgttggg ctaacctacg aagatccttg tatcaacctg caaaaaagtg
    17821 gtgccaatgt ttccattgtt tatccaaaag aaggaacggt gtttgtgccc tcctctgttg
    17881 ctattatcaa acatgcgcca aacatgacag aggctaagct ctttattaat tttatgttat
    17941 cacgtgatgt gcaaaatgcc tttggccaat caaccagtaa ccgacccatt cgtcaagatg
    18001 cccaaaccag tcacgacatg aaagccttag aaacgatagc tactttgaaa gaggattatg
    18061 cttatgttac caagcacaag aaaaaaatag tggctacgta caaccagttg cgccaacggt
    18121 tggaaaaagc taagtaggag ttaaaagtat ggtattgtca acttgaaaaa ggtcctaagg
    18181 ccttttttct atgttataat taagaatcat cttcagttga gaaagtaagg attatgccac
    18241 caaaaactat tgatattgaa aagtaccacc agcttgcttg gcaaaggcag gctgaacacc
    18301 gaaagttttt agcgacttta aagaaaaaac cacctaaaca tctagacaaa attgtccagg
    18361 acattcatca ggaggttttt caagagattg attgcacgtc ctgtgctaat tgttgcaaag
    18421 accttggacc tgatttgaca gagaccgata ttgtcaaaat cgctaaacta ttccgaatga
    18481 aattagcgac ttttgaaaag acttacttgc gcgtggatga ggatgatgat aaggttttcc
    18541 ggttcatgcc ctgtcctttt ttaggagggg ataacctttg aagcacttat gatgtaaggc
    18601 ctaaagcttg ccgagaattc cctcacacag accgtaaaaa aatctaccag attaatcacc
    18661 tcacttcaag aacaccttga tttgtccagc cacctacgag tttgtggagc ggttaagcaa
    18721 acgtttgacc cgttcttagg ctagaaaaag gtgttcttgt agtagaaagt gtatttatta
    18781 tcacttttga caaaaaatct ctttccctaa ttgttctaaa gaggcgacaa tccttaatca
    18841 ctgttaacaa acagttaacc gataagtgga ttaaggtttg tcatctcaaa taaaataccc
    18901 tcaaaaggag accttatgac cacagaattt gacaaaatga ctagaggcga atggtatgat
    18961 gctaattttg attcggaact gattcaaaaa cgcatgatgg cacaagacct ctgtttcgat
    19021 ttaaaccaat taaagcctag tcgagaagaa gaacgctctg ccgttttaaa ccaacttttt
    19081 ggacaatctt ttgagggctt ggtcctgctc agccctttca tctgtgacta tggtaagaac
    19141 attacttttg ggaagaattg ctttattaac agcaattgtt atttcatgga tggggcgaag
    19201 attgccttgg gcgataatgt ctttgtaggt ccttcgacag gtttttacac ggcaaatcac
    19261 cctctagatt ataaacgccg caatgagggg cttgaaaaag ctttaccaat taccatagga
    19321 gataatgtct ggtttggtgc caatgtcaat gttatgcccg gcgttactat tggcagtggt
    19381 tgtgtgattg cgtcaggatc agttgttacc catgatattc cagtcaattc ccttgcggca
    19441 ggtgttcctt gccaagttgt acggaaaatt gagcaggagt agaaagtgag gcaaaaagtg
    19501 cattacaact ttcttttttt cgaccttgac cataccctac ttgattttga tgcggcagaa
    19561 gaggttgctt tgaccaagtt gctagaagag taccaggtta ttgatattaa ggcttacaag
    19621 gactactata agccgatgaa tcagaactta tggaaacagt tagaaggtgg ggatattagt
    19681 aaagctgatt tggttaactc tcgctttgct ctgctatttg ctcattttgg agtgacggtt
    19741 gatggcaggc agttagcaga aggttatcaa aagcatttaa aagatcaagg gcaagtttat
    19801 gctggtgcta aggagctgtt agcggactta actgcccaag gctataatct ttatgcggca
    19861 actaatggca tcgcaacgat tcaacaaggc cgattgcaag cgtctggttt ggcaccttac
    19921 ttcaaagcca ttttcatttc ggaacagtcg ggtagtcaaa aacctaaaaa agctttctat
    19981 gattggatga cccagcaggt ttccaattac caacctgatc aagctttgat gattggtgat
    20041 agtttatcgg ctgatgttca aggtggaatt aatgctggca tggatacctt atggtacaac
    20101 ccaaagcatc tcctgaataa cagccctgtt catcctacct atgaggtgtc agattatcaa
    20161 gctctgctga attgtattgc ggagtaacag acaatagcct ttaaaaatag cgtagtcatt
    20221 ttttgaaatc gatttcataa tctggtagac taaggttatc acgaaaagga ggctatatgt
    20281 gatggcttat caaactattt acccttatac aaatgaagtc cttcatacat ttgataatat
    20341 gaccgatcaa ggtttggcag atgtccttga aagagctcac cttctctata aaaaatggcg
    20401 caaggaagat catttagaag aacgtaaagc ccagcttcat caagtggcta atattcttcg
    20461 tcgtgaccgc gataaatacg ctgaaatcat gaccaaagac atgggaaaac tgttcacaga
    20521 agcccaaggt gaagtaaatc tttgtgccga tattgctgat tactacgctg ataaggcaga
    20581 tgaatttttg atgtctacgc cactagaaac ggattcaggt caagcttact atttgaaaca
    20641 atcaactggt gttatcctag ctgttgagcc ttggaatttt ccttactatc aaatcatgcg
    20701 ggtctttgcg ccaaacttta ttgttggaaa cccaatggtt ttaaaacacg cgtccatttg
    20761 cccacgctct gcccaatctt tcgaagaatt agttcttgaa gctggtgctg aagcaggtag
    20821 tattaccaat ttgtttattt cttatgacca agtatcacaa gtgattgctg ataaacgtgt
    20881 ggttggtgtc tgcttgacag gttccgaacg tggaggagct agcattgcag aagaagctgg
    20941 taaaaacttg aaaaaaacaa cccttgaatt gggtggagat gatgccttta ttatccttga
    21001 tgacgcggat tgggatcaat tagagaaggt gctttacttc tctcgtctct acaatgcagg
    21061 tcaagtatgt acctcatcga aacgttttat cgtgcttgac aaagactatg accgtttcaa
    21121 agagttatta acaaaagtct tcaaaacagc taaatggggt gacccaatgg atcccgaaac
    21181 gacgttggcg ccattatcat cagctcaagc taaagcagat gttcttgacc aaattaagtt
    21241 ggccttggat catggtgcgg agttggttta tggtggtgaa gcgattgatc atccaggtca
    21301 ttttgtcatg ccaaccatta ttgctggcct cacaaaagac aaccctattt actatcaaga
    21361 aatctttggc ccagtcggtg aaatctacaa agtgtcttct gaagaagaag cgattgaagt
    21421 ggcgaatgat tctaactatg gccttggagg tactatcttt agtagcaatc aagaacatgc
    21481 gaaagccgta gctgctaaaa ttgaaactgg tatgtctttt atcaactcag gctggacaag
    21541 tcttccggaa ctcccatttg gtgggattaa acattcaggc tatggtcgtg aattgagtga
    21601 gcttggcttt acatcttttg tgaatgaaca ccttatctat attccgaata aaacgaataa
    21661 tggcaatact aaagtttgat ctagattgtt aaaggagatc tgctcgcctt aataaacaat
    21721 caaggcgtta cactcttctt ttttaaagtc aaaattttaa aaatttgata aactagtaac
    21781 atgaacgaac tgattaaaca taaactagaa ctcttaccag acagccctgg ctgttactta
    21841 cataaagata aagaaggaac tattatctat gtcggtaagg ccaagaattt gaaaaaacgc
    21901 gtccggtcct attttcgtgg gagccatgat accaaaacag agttactagt atctgagatt
    21961 gtagatttcg aatatattgt aacagagtca gataccgaag cgcttttgtt ggaaattaac
    22021 ttgattcaaa aaaatatgcc taagtacaac atcaagttaa aagatgataa atcttatccc
    22081 tttctcaaaa tcacaaatga gtcttttcct cggttagtga ttacaagata tattaagaaa
    22141 aatgatggat tgtactttgg gccctaccca gattcttaca ctgctaatga agttaaaaag
    22201 ttattagata ggatttttcc atttaaaaag tgtaaaaatc ctatcaataa ggtctgtttt
    22261 tattaccatt taggccagtg ctgtgctcat accatttgtc atacagacaa ggcctattgg
    22321 gatcgtttaa ttgatgatgt taagcacttt ttaaatggta aagatgacaa aattattgaa
    22381 gatcttcgtt ctaagatgtt agcagcttct aaagaaatgg catttgaacg tgccgctgaa
    22441 tatcgagatt tgatttcagg tattgccacc atgcgcacca agcagcgggt gatgagcaag
    22501 gatttgcaag atagagatat ctttggctat tatgttgata agggatggat gtgtgtccaa
    22561 gttttctttg tgcgtcaagg gaaattaatt cagcgtgatg ttaacctttt tccctattat
    22621 accgatgcag aagaagattt cttaacttat atggggcaat tttaccaaga taaacagcat
    22681 ttcatcccta aagaagtctt tattccagaa gctattgatg aagaactggt agccgctatt
    22741 gtgccaacca aaatcatcaa acccaaacgc ggtgaaaaaa aacaactggt agctttggct
    22801 actaagaatg ctcgtgttag tctgcaacaa aaatttgatt tattagaaaa agatatcaaa
    22861 aaaacgagtg gtgctatcga aaatttaggt cagttactta ggattgacaa gcctgtgcgc
    22921 attgaagcct ttgataattc taacattcaa gggacaagtc cagttgcggc tatggttgtt
    22981 tttgttgatg gcaaacctag taagaaagat tatcgtaaat ttaagattaa aacagtggtg
    23041 ggaccagatg attatgccag tatgagagaa gtgctttttc gaaggtacag tcgggtgaaa
    23101 aaggagggac tgcaagctcc taacttgatt attgttgatg gaggcgttgg acaagtcaat
    23161 gtggccaaag atgtgataga aaaacaactt ggactcacta ttcctgttgc gggacttcaa
    23221 aaaaacgata agcaccagac ccatgatttg ctttttggaa acccacttga agttgtaccg
    23281 ttgccgcgcc gttctgaaga gtttttctta ctgcatcgga ttcaagacga agtgcatcgc
    23341 tttgcagtta catttcatag acaagtgcga cgtaaaaatt ctttttcatc aacattagat
    23401 catatttcag ggcttggccc aaagcgaaaa cagttattac taagacactt taaaaccata
    23461 acagcgattg catcagctac ctctgaggag attcaagctt taggtatacc taaaacagta
    23521 gttgaagcaa tacagcaaca gataacagac aataaaaatg atagatcgtc cccttaaata
    23581 agtgttatgt tttcagtact gatgaggtaa aaaggcgcta cttgttacgc acgacatttc
    23641 agttcgttat tttgaaactt tcagaaggtt gcgaagtgta gtgagtttat gattttaaaa
    23701 actattttat ggtaaaataa aatggtatta aaaaaaacag atttagatag gaagttttga
    23761 aatgaaattt ctagagttaa acaaaaaacg ccatgccatt aagaccttta atgaccagcc
    23821 agttgactac gaggatttgc gaacagccat tgagattgca accttagcac ctagtgccaa
    23881 taacattcaa ccttggaaat ttgtagtcgt tcaagagaaa aaagctgagt tagcaaaagg
    23941 gttacctcta gcaaataaag tgcaggttga acaggctcaa tatgtagtgg cgctattttc
    24001 agacactgat ttagctttac gttctcgtaa aattgcccgt atcggtgtca aatcattacc
    24061 agacgactta attggttatt atatggagac tttgccacca cgttttgctg cttttaatga
    24121 ggttcaaacc ggtgagtact tagctattaa tgcaggcatt gtggctatga acttggtgtt
    24181 atccttgacg gatcaaaaga ttgcttccaa tattatcctt ggttttgata agtcaacgac
    24241 caatgagatt ttagacatcg atccgcgttt tcgtccagaa ttattgatta ctgttggcta
    24301 tagtgacgag aaaccagaac caagttatcg tttgcctgtt gatgaagtca tcgaaagaag
    24361 atagagtcag gacaattagt taaatgtaat gtccagatgc gctactgagc gaccactatt
    24421 gttagactga tactattttg gcttatttta agttaaacat tatccctaaa acgaataagg
    24481 agagtataat gacaaccatt gattttaaag cagaagttga caaacgtaaa aaagctatgc
    24541 tagcagattt agttgaccta ttacgcatta attcagaacg cgatgaccaa ttagctgatg
    24601 ataaacaccc atttggccca ggtccagtta aggccttgga gcatttcctc gcaatggctg
    24661 agcgtgatgg ctacaaaacg cgtaatattg ataactatgc aggtgacttt gaatttggtc
    24721 aaggtgatga agtactagga atctttggtc accttgatgt tgtgccagct ggtagcggtt
    24781 gggatacaga cccttatgag ccagtgatta aagacgatcg catctatgcg cgtgggtctt
    24841 cggatgataa aggaccaacc atggcgtgtt actacgctct gaaaatcatt aaagaactgg
    24901 gtcttcctgt gtctaaaaaa gttcgtttca ttgttggtac cgatgaagaa tctggctggg
    24961 gggatatgga ttattacttt gctcataatg gcttgaaaaa tccagatttt ggtttctcac
    25021 cagatgctga atttccaatt atcaatggtg agaaaggaaa tatcactgaa tacctccatt
    25081 tcgctggtga caacaagggc gcttttgtgc ttcatcgctt ccaaggtggg ttgcgtgaaa
    25141 acatggtacc agaatctgcc acagctgtca ttacggcacc gcatgacctt gatgtgctag
    25201 aagcagcttt ggagcaattt ttatcagaac atggtgttaa aggttctatg aaggctactg
    25261 atggtaaaat tgaagtaact attattggta agtctgctca cgggtcgact ccagaagcgg
    25321 gtgttaatgg tgctaccttg ttggctaaat tcctgaatca atttaccttt gaaggggctg
    25381 ctaaggatta tttacatgtg gcaggtgagg tgcttcatga agactttgcg gcagaaaaac
    25441 taggccttgc ttatactgat gaccgtatgg gagccctgag catgaacgca ggtgtcttca
    25501 cctttgatag tcaatcagct gacaatacca tcgccttgaa tttccgctat cccaaaggaa
    25561 ctgatgcggc aaccttaaaa gcaggattgg aaaaattacc aggattaact aaggtaagcc
    25621 tttctgagca tgaacataca cctcattatg tgccaatgga tgatgaatta gtagctacct
    25681 tacttgctgt ttacgaaaaa caaacaggtc ttaaaggcta cgaacaagtt atcggcggag
    25741 gaacctttgg gcgcttgcta gaacgtggtg tggcctttgg agcgatgttc ccaggtgatg
    25801 aaaacactat gcaccaagct aatgagtata tgcctttaga aaatatttat cgctcagcag
    25861 ctatttatgc tgaagcaatc tacgaattaa ttaaataaga tgcttatgac agcaaaaaga
    25921 gctggaacac tgttccagct cttttgtgat gattagagac tgttgttatt taccaagaca
    25981 gaattgacta aagagttggg taatgagttc atcaggagca gcatcccctg tgatttctcc
    26041 gagaatttcc caagttctgg ttaaatcaac ctgtagtaaa tcaactggca tgccaagcgc
    26101 caaaccgtca ttgacagctt ctaaactctg gactgccttt tcaatcaaag aaatgtggcg
    26161 ggcatttgac aggtaagtgg catcttgctc taccaaacca gcgttgtcaa agaaaagctg
    26221 gttgatacga tcttcgatta gattaatgtt ctggttggtt aaaacggaaa tgggaatgta
    26281 gtcatctggt aattgctcta attcaatctt ctgttccaaa tccgttttat tcaacaatat
    26341 aatacggtta ctgtcctgac tgagatttaa caaagcccgg tcttggtcag tcagtttttc
    26401 agaagcattc agcacaagaa gaacaaggtc agcttcttga agagcttttt tagaccgttc
    26461 aactccgatt tgttcgacca gatcatctgt ctcacgaata ccggccgtat cgacaagttt
    26521 tagaggaact cccttgatat taacgtattc ttcaatgaca tctcgggtgg ttcctgcaat
    26581 atcagtcaca atagccttgt cttctcgcaa caaattatta agtaagcttg acttaccgac
    26641 gtttggacga ccaataatag ctgttgataa gccctcacgc agaattttac cgcgtttagc
    26701 tgttcgaagg agactttcca aaaggctttg gaattcttgt gttttctcac gtagcagagc
    26761 agtggtcatt tcttccacat cgtcgtattc agggtagtca atattgactt cgacttgagc
    26821 taaggtgttg agaatttcct gacgggtgtc attgataagc tgggaaaggg agccatccag
    26881 ttgcttgact gcaatcgtca tagccttgtc tgttttggca cgaataatgt ccatgacagc
    26941 ctcagcttga gtcaaatcaa ctcgcccatt taagaaggca cgtttggtga attcgccagg
    27001 ctctgccatt ctagcgcctt ggcgaatcaa tagttgcaag atttcatttg tcacagcaat
    27061 accaccgtgc gtattgattt caaccacatt ttcacgggta aaggttttgg gggctagcat
    27121 gactgacacc ataacttcat cgataatagt tcctgtctta ggattaatga tatggccata
    27181 gttaatggtg tgtgaggcga cttgctctaa gttctttccc ttaaaaacag attgtgcaat
    27241 ggcaagcgca tctgtccctg ataaacgaac aatgccaata gctccttcac caagaggggt
    27301 tgaaatggcg gtaattgtat caaattcttt agttatactc atgccctcta ttttaaatca
    27361 agtcctagta gaaagcaagg tgttatttgt atagaaaata ggactagcac cattttgagt
    27421 ttagctaata ctaaaaaaat aaatgtaaaa aaatcatttt ttaatagttt ttattgacaa
    27481 tagtattata aaagaatata atgatatagg ttttgataaa aaactgacct aagacagcag
    27541 gggagcatgc tcataatatt cctgccgagg cacaaaacgt gattaaagaa aataacgtat
    27601 ttgtactttc gtgtctaggt ttaggtgcga ttttttttgt acctagccca aaaataaaaa
    27661 cggaggtaaa actaatgagt gaagcaatta ttgctaaaaa agctgaacaa gtcgaactta
    27721 ttgctgagaa aatgaaagca gcagcaagta tcgttattgt tgattcacgt ggtcttacag
    27781 ttgatcaaga tactgttctt cgtcgttcac ttcgtgaaag tggcgttgag ttcaaagtta
    27841 tcaaaaactc aattttgact cgtgcagctg aaaaagcagg tcttgatgag ttgaaagatg
    27901 tattcgtagg accatctgca gtagcatttt ctaacgaaga tgttatcgca ccagctaaag
    27961 ttatcaacga ctttactaaa actgctgacg cacttgaaat caaaggtggt gcaatcgaag
    28021 gcgctgtttc ttcaaaagaa gaaatccaag cacttgcaac attgccaaac cgcgaaggaa
    28081 tgctttctat gcttctttct gtacttcaag ctccagtccg taacgttgca tacgctgtca
    28141 aagctgttgc agaaaacaaa gaaggcgctg cttaagtcgt ctcagagtag cttaaggcta
    28201 cgcttaacat tttaaattaa ttatttggag gaaatcacaa tggcattgaa cattgaaaac
    28261 attattgctg aaattaaaga agcttcaatc cttgagctta acgatcttgt aaaagctatc
    28321 gaagaagaat ttggtgtaac tgcagctgct cctgtagctg ctgcagctgc tggtggtgct
    28381 gaagaagctg ctaaagattc attcgacgtt gaattgacat ctgctggcga caaaaaagtt
    28441 ggcgttatca aagctgttcg tgaaatcaca ggtcttggtc ttaaagaagc taaaggtctt
    28501 gttgatggag cacctgctaa cgttaaagaa ggcgttgctg ctgcagaagc tgaagaaatc
    28561 aaagctaaac ttgaagaagc tggagcaaca atcactctta aataagaagc agatagcaat
    28621 ttgagagcga aatcccgatt tatcagtctt ttagagttta tagcgatttg aagaaactga
    28681 taaattgatt tggtttcttg ccaaaaatcc tgcccaaaaa atctttggca ggatttttat
    28741 tttctataaa aattaagctg agtagagtgg ttattctatc tatatgtgtc tagtaataat
    28801 ttttttatgt gaacgagaac attttaataa gtgttctcat ttttttattt tcaggaggaa
    28861 aatcatgcaa atgaatgaac agtcatcacg acaaatggtg attgccatct atctcattta
    28921 ttccaactca aaatgacagt actccttggc tgttccaagt cgaaattgtt agcgattaag
    28981 aaaacctacg agactatggt ttgatcgatg aagtccaaca gtctcccagt gaaaaagggc
    29041 gtttggctaa taagatttat ttaggaaagt tggaacatga tttcacccca gtcttaagtt
    29101 cagacggggc cagtctcaaa aacagaccag ccccgtgtta aattcagccc ctagtgagac
    29161 tgaaaagagt gaaactgaag gtagtgaaac taaagagagt aatttagtga ttgaggacga
    29221 ggaggaaaga aaggagtgca ctagtgtgaa aaagactgag ggtcacttta cccgtcaagt
    29281 tgatcaggtc accaaatatg acaaagatta tatttggagt ttggttcatt cccaattacg
    29341 tgaaggtggt ttatcacaag ctgctagtga cttagtcatg agctattttg aagaacgcta
    29401 tgcttatgcg ctggaacaca tacggttcgc aagaacagct gaagctattg cggaatatgt
    29461 ctttaatggt gtcttatccg aatggaccaa gcaactaagg tgacaagaag cgaagtgagt
    29521 aagatgtttt ggtggctatt attaggtgtt tggggactaa ggacaatttg gttgattatt
    29581 gaagttatca ctgcaccaga aatggaagat tatagttgag taaggacgtt acataagcag
    29641 taaggtcctt ttattttgtc agaaaggaat gagagatatg aagtttttag atttgtttgc
    29701 aggaattgga ggctttcgat tgggtttaat taatcagtgc catgagtgta ttggcttttg
    29761 tgagattgat aagtttgcga ggcaatctta caaagccatc tatgagaccg aaggagaaat
    29821 agaatttcat gacattagac aagtcacaga ccaagacttt agacaactta gagggcaagt
    29881 ggatatcatc tgcggtggtt tttcccttgt caagcatttt cactcgcagg cagacgactg
    29941 ggatttgagg gcactaggag aactctcttc tttgagatta ctcgagcgac caaacagata
    30001 aaaatcatac ccataatcac atccaatcaa cgccattgac cgtaattcag ataaaaaatt
    30061 gatatggaat tatgccttgg aacgaaacct ccgtatgatt tcagaccgta tttcaaaaat
    30121 gactggagca aaaattatcg agaagcgtta ttcctaccgt gactatcaaa aatataggta
    30181 gtctagtcat aaatttgaat tgaaacaacg tctttatttt ttgatgcagc agtcaaagtt
    30241 ctttgatgat tttttagaaa aagcagagta attacatgtt catattgatt ttagtcagaa
    30301 gcatagcaga ttcatgatga cggatcgagt agtgaaaaaa ccaattcgag gccgtcaact
    30361 cagtaaacga gatttatatg atgaagaatt ttttcgtaca cattttgcca agatagaaat
    30421 tgaaagtcga ttagaatttt tgttgaaccg tgccaattca ttagaggagc tactattaaa
    30481 agcaaaagaa gtgaatctaa ccattgactt aaaacaaaaa atgtaacttt tatcctagaa
    30541 gaagatactc aaaagataag tttaggtcat aaaaaaataa gcgataagaa attatatgat
    30601 gtcaattttt tcaagattac tttaaaaata aggaagtcgg tgtttcagat cgattagaaa
    30661 gtctacagaa acagtacatg cttttcaaga agaacgagat aaagaggagg tatccactga
    30721 agagagtaag gaagtcttca agaaatacaa ggggcaacga gatgccatcc atgagtttga
    30781 agttgaactt gtggaacatc aaattgagag gttaattgct gaggacattt atatcaaggt
    30841 gtcttttagt gtaaagcaga gtggtcttat tttcattcct aactatcaat tggatattag
    30901 gaaagaagaa aatcataaaa aatataaagt ctatattcgt gagaccgcac aattcttcat
    30961 ttacaacaag gaggcgtctg aacttaatcg ctatatgcga gggcatgagc tcatttgcca
    31021 gttaaccaat gatagcaaga gtattccaaa aagaagacga caaaccattg atacacttaa
    31081 gaaaaagatt gaagaaataa gtctcctcat tgagttagat acggagaata agccttatca
    31141 agatattaaa gatgacattg ttaaagatat ggctcaatta gatttgacca ttacagagtt
    31201 gcaggaccat attgctcatc tcaataaggt tgcggaggtc ttgcttaatc tgaacaacaa
    31261 cgatatagag aaccgccgtt tggccagata tgactatgcc aagatgaact tgactgcagc
    31321 tataaaaata gaagaagttg agaaagagat tgaaacttct caaaatgaac ttaatatatc
    31381 catagatgag tatgaatatc tagtaagaag gttggaaaag tttggagaga tcttgagtga
    31441 tagcaaaatt atcgatactt ctcgaaatga aatacaatgg gagtaattat gatataattt
    31501 gggtggtagc aatttttata tcagatatta aaatctagtt gatgtttcgt aaataactat
    31561 attaaagaaa tgattcctgc cgcttacatt aagaata
//
LOCUS       DAXWIQ010000018        29425 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000018 DAXWIQ010000000
VERSION     DAXWIQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29425
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.018"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            386..754
                     /locus_tag="ACGEUI_000836"
     CDS             386..754
                     /locus_tag="ACGEUI_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165683.1"
                     /translation="MTKKNHGVFWGLLLGSAAASIAYLSLSSSKKDQLLKDSAKKIDD
                     LNAYLQDKSKQVLDAVSEKVQESKDAVEVYGGIAAETVEESLGQAKEKVEGIGEATSQ
                     TIQSKMEKLTSDKTDADDEK"
     gene            complement(804..1715)
                     /locus_tag="ACGEUI_000837"
     CDS             complement(804..1715)
                     /locus_tag="ACGEUI_000837"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8165684.1"
                     /translation="MKTVRIFYNPNSGKKESQLARQVKDYFCQHGFSEDSVKVITPKD
                     ADQAFQLAKQAAKDKIDLVIPLGGDGTLNKIIGGIYEGGAHCLIGLVPSGTVNNFAKA
                     MHIPLQITEALDTILTGQIKQVDICKANQQYMISSLTLGLLADIAADVTAEEKRRFGP
                     LAFLKDSIRILKRNRSYAISLISHNHRIHLKTKFLLITMTNTIAGFPSFSPGAQADDG
                     YFQVYTMKKVSFFKFLWHINDFKQGDFSKAEEISHFQANTLSLLPQAKKQAILPRTRI
                     DGDKSDYLPIQLDIIPKAVSIIVPTED"
     gene            complement(1832..2482)
                     /locus_tag="ACGEUI_000838"
     CDS             complement(1832..2482)
                     /locus_tag="ACGEUI_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFF8165685.1"
                     /translation="MNQTFKQSLPLSFSEEVANSVTHAIGAFAMLILLPISASYAYQT
                     YDLKAAIGISIFVISLFLMFLSSTIYHSMAYGSVHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVSGWLGYIIIVLQWGITLFGILYKIFAKRINEKFSLMLYIVMGWLVVFILPVII
                     QKTSLAFGLLMLFGGLSYTIGAVFYAKKRPYFHMIWHLFILLASALQFIAITFFML"
     gene            complement(2479..2919)
                     /locus_tag="ACGEUI_000839"
     CDS             complement(2479..2919)
                     /locus_tag="ACGEUI_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFF8165686.1"
                     /translation="MIFPSLPYWKDLPDLDLYLDQVLLYVNQCTDFSEVSDNKSLTAS
                     MINNYVKHGYVTKPIKKKYQKQQLARLIAISLFKTVFPIQDISRVLEELQAQADSESL
                     YNTFVTCWNQKAPIEEDIPPIVQVACQTVKDYHKTIYLLQEVTQ"
     gene            3138..3986
                     /gene="ylqF"
                     /locus_tag="ACGEUI_000840"
     CDS             3138..3986
                     /gene="ylqF"
                     /locus_tag="ACGEUI_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFF8165687.1"
                     /translation="MAMIQWFPGHMSKARRQVQENVKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADATRTKEWKAYYESQGIKTLAINSKEQSTVKKVTEAAK
                     ELMADKIQRLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFREYY
                     PNRLTKRFKNIPLEEEAPEIIMTLTRQLGFKDDYDRFYTLFVKEVRDGKLGQYTLDQV
                     GDMDAD"
     gene            3976..4767
                     /locus_tag="ACGEUI_000841"
     CDS             3976..4767
                     /locus_tag="ACGEUI_000841"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFF8165688.1"
                     /translation="MPTSIKAIKESLEAVTSLLDPLFQELATDTRSGVQKALKSRQKV
                     IQAELAEEERLEAMLSYEKALYKKGYKAIAGIDEVGRGPLAGPVVAACVILPKYCKIK
                     GLNDSKKIPKAKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLE
                     GQLTQPDYLLIDAMTLDIAISQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFP
                     GYGFAKNAGYGTKEHLQGLKAYGITPIHRKSFEPVKSMCCDSTNP"
     gene            4832..5668
                     /gene="dprA"
                     /locus_tag="ACGEUI_000842"
     CDS             4832..5668
                     /gene="dprA"
                     /locus_tag="ACGEUI_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFF8165689.1"
                     /translation="MNHFELYKLKKAGLTNKNILNILDYQKHQEKSLSLRDMAVVSGC
                     KHPSHFIEAYKQLDIQNLKMEFKQFPSISILDKHYPMALKEIYNPPVLLFFQGNLDLL
                     EKPKLAIVGSRRSSDTGVKSVRKILKELGNRFVIVSGLARGIDTSAHLACLKNGGQTI
                     AIIGTGLDRFYPKENRELQTFLGKNHLVLTEYGPGEEALSYHFPERNRIIAGLSRGIL
                     VVEAKNRSGSLITCQIGIEEGRDIFAVPGNILDGKSEGCLQLIKEGATCVTSGMDILS
                     EY"
     gene            5775..7904
                     /gene="topA"
                     /locus_tag="ACGEUI_000843"
     CDS             5775..7904
                     /gene="topA"
                     /locus_tag="ACGEUI_000843"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFF8165690.1"
                     /translation="MGTKTTTKPKTGTKKSTTKKKSTAKKNLVIVESPAKAKTIEKYL
                     GRSYKVVASVGHIRDLKKSSMSIDFDNNYEPQYINIRGKGPLINSLKKEAKAAKKVYL
                     ASDPDREGEAISWHLSHILGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQ
                     ARRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENDIKAFVPKEYWSIDG
                     LFKKGTKKFQATFYGINGKKTKLDNNNDVKEVLAKLTNEDFLVSKVDKKERRRNAPLP
                     YTTSSLQQDAANKINFRTRKTMMVAQQLYEGIHLGENGTQGLITYMRTDSTRISPVAQ
                     NDAAQFIINRFGANYSKHGNRVKNTSGVQDAHEAIRPSSVNHTPDSIAKYLNKDQLKL
                     YTLIWNRFVASQMTAAVFDTVKVNLEQNGVIFVANGSQMKFDGYMAVYNDSDKNKMLP
                     EMAEGETVKKISTSPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTLEVIQRRYY
                     VKLSAKRFEPTELGEIVNKLIVEFFPDIVDVAFTAEMEGKLDQVEIGEEQWQHVIDQF
                     YQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGHPMVIKLGRFGKFYACSNFPECRNT
                     KAITKEIGVTCPVCHKGQVIERKTKKNRIFYGCDQYPDCEFISWDLPIGRACPKSGDY
                     LIEKKVRGGKQVMCSNETCDYKEEKIK"
     gene            complement(7979..8861)
                     /locus_tag="ACGEUI_000844"
                     /pseudo
     CDS             complement(7979..8861)
                     /locus_tag="ACGEUI_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            8874..9419
                     /locus_tag="ACGEUI_000845"
                     /pseudo
     CDS             8874..9419
                     /locus_tag="ACGEUI_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            9434..10426
                     /locus_tag="ACGEUI_000846"
     CDS             9434..10426
                     /locus_tag="ACGEUI_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFF8165691.1"
                     /translation="MKLKLYNVRGEEAVLAKKWADDNGIEISLTESPLTPETVKEAEG
                     FDGIANAQIGPLDDAIYPLLKEMGIKQIAQHSASVDMYNLDLATENDIIITNVPSYSP
                     ESIAEFTVTIVLNLIRHVELIRENVKKQNFTWGLPIRGRVLGDMTVAIIGTGRIGLAT
                     AKIFKGFGCKVVGYDIYQSDAAKAVLDYKESVEEAIKDADLVSLHMPPTAENTHLFNS
                     DLFKSFKKGAILMNMARGAVIETQDLLDALDAGLLSGAGIDTYEFEGPYIPKNFEGQE
                     ITDSLFKALINHPKVIYTPHAAYYTDEAVKNLVEGALNATVEIIKTGTTTTRVN"
     gene            10531..11196
                     /locus_tag="ACGEUI_000847"
     CDS             10531..11196
                     /locus_tag="ACGEUI_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HFF8165692.1"
                     /translation="MTYIALIGDIIQSKQLTDRSKVQKTLAAYLDDLNKTFAPYIISK
                     LSLTLGDEFQGLFQVDTPIFHLIDLINHHMDIPIRFGVGVGSILTDINPDISIGADGP
                     AYWHAREAIRYIHQKNDYGNTTLALRTGHHNQDDVLNSLLAAGDAIKANWRASQWEIF
                     DTLLDLGIYEEYFDQQRLGKQLSLSSSALSKRLKSSHVKIYLRTRQSALNCLKQIKEE
                     TDD"
     gene            11189..11905
                     /locus_tag="ACGEUI_000848"
     CDS             11189..11905
                     /locus_tag="ACGEUI_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HFF8165693.1"
                     /translation="MINGVSHYLAQTPTLTLFLICHVLSDYQLQSQQVADLKEKHLTY
                     LGYHLIGVSIPLICLTLIIPQAWLMSLLVMISHALIDWLKPKMANSLKWKREWIFLLD
                     QCLHIAISSFAGLRLAGVTLPNWLPISILMTVLFILLITKPTNIVFKLFFSKYQPDQG
                     EKMDTIIGAGATIGILERIVIGVCMIMGQFASIGLVFTAKSIARYNKISESPAFAEYY
                     LIGSLFSILSVFIAAWICFF"
     gene            12056..13402
                     /gene="trmFO"
                     /locus_tag="ACGEUI_000849"
     CDS             12056..13402
                     /gene="trmFO"
                     /locus_tag="ACGEUI_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HFF8165694.1"
                     /translation="MSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATP
                     QHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDR
                     EGYAESVTAELENHPLIEVIRGEITEIPDDAITVIATGPLTSDALAEKIHALNGGDGF
                     YFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPL
                     NAFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQ
                     LRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLL
                     TETFQSRSNPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKREEALIFPQTTAIGS
                     LPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIASRALADLDTCLASL"
     gene            13558..14961
                     /locus_tag="ACGEUI_000850"
     CDS             13558..14961
                     /locus_tag="ACGEUI_000850"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HFF8165695.1"
                     /translation="MQFGGFSMQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTILD
                     KIGYYSLECWGGATFDACIRFLNEDPWERLRTLKKGLPNTRLQMLLRGQNLLGYRHYA
                     DDIVDKFISLSAQNGIDVFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVHT
                     LNYYLSLVKELVEMGADSICIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSGI
                     SQMTYLAAVEAGADRIDTALSPFSEGTSQPATESMYLALKEASYDITLDETLLEQAAN
                     HLRQARQKYLADGILDPSLLFPDPRTLQYQVPGGMLSNMLSQLKQANAESKLEEVLAE
                     VPRVRKDLGYPPLVTPLSQMVGTQAAMNVILGKPYQMVSKEIKQYLAGDYGKTPAPVN
                     EDLKRSQIGSAPVTTNRPADQLSPEFEVLKAEVADLAQTDEDVLTYALFPSVAKPFLT
                     TKYQTDDVIKVTAFIKA"
     gene            15004..15318
                     /locus_tag="ACGEUI_000851"
     CDS             15004..15318
                     /locus_tag="ACGEUI_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165696.1"
                     /translation="MTYSFIDILQVTLVSLFLVLTCLTGIMGLMMLSGKLLSQEKKTL
                     SQQASSIPQEQEADKLSGEALETLLAKDELARIAVMTSLAYASQEESGKRFEVIAVER
                     KS"
     gene            15318..15668
                     /locus_tag="ACGEUI_000852"
     CDS             15318..15668
                     /locus_tag="ACGEUI_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HFF8165697.1"
                     /translation="MKRYEVTVNGQVYEVSLRELVDGETVEVSQPAAPATEKEMNANA
                     AGGGIQVKAPMSGTVLSIFATEGKAVKKGEAVLVLEAMKMENEILAPADGLVSKIHVV
                     ANQTVESEQVLISF"
     gene            15678..16808
                     /locus_tag="ACGEUI_000853"
     CDS             15678..16808
                     /locus_tag="ACGEUI_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HFF8165698.1"
                     /translation="MLDVLNQMVQSSGLAHLTVNNLIMICLASFFLYLGIKKEYEPYL
                     MVPIAFGILLVNLPMAGLMDHPANGNPGGLLYYLYKGTSLGIYPPLIFLCLGASTDFG
                     PLIANPKTILLGGAAQVGIFLAFFLAIMLGMTPQEAASVGIIGGADGPTAIYVTTKLA
                     PDLLSTIALAAYSYMALVPIIQPPIIKLLTTKAERQVKMTQARTVSQKEKIIFPIMVT
                     IFVSLLVPSATTLVGCLMLGNLVREIKIVPKIVENLQQVVMFCITIILGLTVGAKANG
                     DLFLSATTLKIIALGLIAFAAGTAGGVLMGKVMYYLSGNKVNPMIGAAGVSAVPMAAR
                     VVQKIGQEEDPSNFLLMHAMGPNVAGVIGSAIASGALLAFFG"
     gene            complement(17008..17892)
                     /gene="citG"
                     /locus_tag="ACGEUI_000854"
     CDS             complement(17008..17892)
                     /gene="citG"
                     /locus_tag="ACGEUI_000854"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFF8165699.1"
                     /translation="MTKAVLTSISQLALKALLYEVSLSPKPGLVDRFDNGAHDDMSFI
                     TFIDSMIALSPFFQAYIETGFAYAKEEPLLLFNRLRQLGQKAEETMFCATQGINTHKG
                     LNFSMALLLGATGAYLARTPHLMTDLGRFSKEDTLAICRLVKPMTAHLIQTDLGHLNT
                     KKEFTYGEQLFVTYGIKGPRGEASEGFTTLTDHALPYFRQMISQNDPETSQLRLLVYL
                     MSIVEDGNLIHRGGIEAWKGVKADMRLLLQQDLSTTDLRLALSSYNQCLINQHLSPGG
                     AADLLALTFYFAFLEKLL"
     gene            complement(17885..18580)
                     /locus_tag="ACGEUI_000855"
     CDS             complement(17885..18580)
                     /locus_tag="ACGEUI_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8165700.1"
                     /translation="MNPIIEAVKQNLDLSRNIPLKIAFYNALKKTIILRQIPVGSRIN
                     EKEFSIALNISRTPIRYALGLLSEEHLVEHIPKKGIIVKGVSIKDACEIFEIRKALET
                     LATVQAMHLMTEEDFKVMHNLLEDCETFIAEDDTNRILDNFNAFNNLIYSYSQMVRLK
                     EIVTELQAYLVYFRKISISSVERRKRALSEHWMIYRGMKNKDHEQITLITHEHLNSSL
                     EFILKEMRPEQND"
     gene            complement(18738..20141)
                     /locus_tag="ACGEUI_000856"
     CDS             complement(18738..20141)
                     /locus_tag="ACGEUI_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFF8165701.1"
                     /translation="MLLTMLAYAMIIVFMYVVMKKKMTPFTALVMIPLIMTIAVILTG
                     SADFNADAKFVAFVGDGGIAKDLTAIGPMVMYGINNTAKTGIMLLFAILFFSVMLDAG
                     LFDPITEKMIRFAKGDPMKVLIATAVVAAAVSLNGDGTTTTLICCSAFLPIYKKLDMK
                     IMNLGVLIILQNTIMNLLPWGGPTARAMSVLGVGPEILGYLAPGMILSLLYVICWVAP
                     SMGRKERARLGVIDLSEEDMRQLTDITDPDTLFIRRPKNFVFNAILTIGLITWLVAGS
                     FNKSIAMAPLLLFAVGTCIALMVNYPVLKDQSKRIGDNAGDAVQVVILVFAAGIFMGL
                     FQGSGMASALAQSFATIIPKQLAGFWGLVIALVSAPGTFFISNDGFYYGILPVLAEAG
                     AEYGFSNMAMALASLMGQAFHLLSPLVAFIYLLLRLTGLDMGEWQKEAAKYALIIFVI
                     FVVTIIAMGQMPLYIPQ"
     gene            20521..20844
                     /locus_tag="ACGEUI_000857"
     CDS             20521..20844
                     /locus_tag="ACGEUI_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165702.1"
                     /translation="MQFLQKWFKHSKKEKLKESSSLSTEIEPSENWAKIPAYIPADKS
                     DYKKVTLITSVIAAGDRPNSQFKVKRILKRNPEAITVSLIASSIAAGVYPESQFRVTS
                     IYCKR"
     gene            20901..21296
                     /locus_tag="ACGEUI_000858"
     CDS             20901..21296
                     /locus_tag="ACGEUI_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HFF8165703.1"
                     /translation="MLRKFKITIDGKEYLVEMEEIGAPAQAAAPAQPISTPVPVPTEA
                     SPQVEEAQAPQPVAAAGADAIPSPMPGTILKVLVAVGDQVTENQPLLILEAMKMENEI
                     VASSAGTITAIHVGPGQVVNPGDGLITIG"
     gene            21341..22462
                     /locus_tag="ACGEUI_000859"
     CDS             21341..22462
                     /locus_tag="ACGEUI_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HFF8165704.1"
                     /translation="MDTLIAGITSITIPQIVMMVIGALLMYLGIKKEYEPTLLVPMGL
                     GTILVNFPGSGVLTQVVNGVEQEGVFEALFNFGIGTELFPLLIFIGIGAMIDFGPLLQ
                     NPFMLLFGAAAQFGIFFVVVVAVLAGFDIKEAASIGIIGAADGPTSIFVANQLAKDLL
                     GPITVAAYSYMALVPIIQPFAIKLVTTKKERRIRMTYKAENVSQMTKILFPIIITLVA
                     GFIAPISLPLVGFLMFGNLLRECGVLDRLSQTAQNELVNIISILLGLTISIKMQADLF
                     LNVQTLLIIVFGLLAFIMDSIGGVMFAKFLNLFRKEKINPMIGAAGISAFPMSSRVIQ
                     KMATDEDPQNFILMYAVGANVSGQIASVIAGGLLLAYFS"
     gene            22480..22614
                     /locus_tag="ACGEUI_000860"
     CDS             22480..22614
                     /locus_tag="ACGEUI_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG-related small transporter subunit"
                     /protein_id="HFF8165705.1"
                     /translation="MTINMDHLISSFELMALGMAGVFIVLGILYLVAEILIKLFPVSK
                     "
     gene            22650..22958
                     /gene="citD"
                     /locus_tag="ACGEUI_000861"
     CDS             22650..22958
                     /gene="citD"
                     /locus_tag="ACGEUI_000861"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFF8165706.1"
                     /translation="MDIKQTAVAGSLESSDLMITVSPNDEQTITITLDSSVEKQFGNH
                     IRQLIHQTLVNLKVTAAKVEAVDKGALDCTIQARTIAAVHRAAGIDQYDWKEIDSWNV
                     "
     gene            22946..23833
                     /gene="citE"
                     /locus_tag="ACGEUI_000862"
     CDS             22946..23833
                     /gene="citE"
                     /locus_tag="ACGEUI_000862"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFF8165707.1"
                     /translation="MERLRRTMMFVPGANAAMLRDAPLFGADSIMFDLEDSVSLKEKD
                     TSRALVHFALKTFDYSSVETVVRVNGLDSCGALDIEAVVLAGVNVIRLPKTETAQDII
                     DVEAVIERVERENSIEVGRTRMMAAIESAEGVLNAREIAKASKRLIGIALGAEDYVTN
                     MKTRRYPDGQELFFARSMILHAARAAGIAAIDTVYSDVNNTEGFQNEVRMIKQLGFDG
                     KSVINPRQIPLVNEIYTPTKKEIDHAKQVIWAIREAESKGSGVISLNGKMVDKPIVER
                     AERVIALATAAGVLSEEDI"
     gene            23836..25368
                     /gene="citF"
                     /locus_tag="ACGEUI_000863"
     CDS             23836..25368
                     /gene="citF"
                     /locus_tag="ACGEUI_000863"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFF8165708.1"
                     /translation="MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPG
                     DSKLLGSVREAIEKTGLTDGMTISFHHHFREGDFIMNMVLEEIAKMGIKNLSIAPSSI
                     ANVHEPLIDHIKNGVVTNITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDI
                     HIDVAFLGAPSSDAYGNVNGTKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPI
                     SIPQTDVDYVVTVDAIGDPQGIAKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSF
                     QTGTGGASLAVTRFMREAMIKENIKASFALGGITNAMVELLEEELVEKILDVQDFDHP
                     SAVSLGKHAEHYEIDANMYASPLSKGAVINQLDTCILSALEVDTNFNVNVMTGSDGVI
                     RGASGGHCDTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVIVTEVGIAINP
                     NRQDLVDHFKSLNVPQFSIEELKEKAYAIVGTPERIQYGDKVVALIEYRDGSLMDVVY
                     NV"
     gene            25361..25939
                     /gene="citX"
                     /locus_tag="ACGEUI_000864"
     CDS             25361..25939
                     /gene="citX"
                     /locus_tag="ACGEUI_000864"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFF8165709.1"
                     /translation="MCKDTYFSGEAIQLSDMLRAREERALRQLHLLKEYPEGSLLSVT
                     MNIPGPIKTSPKLLEAFDIVIKAIQTALADDKICYQLRLLPTTGYEYYLITSLPSRDL
                     KLKMIALETELPIGRLMDLDVLVLQNDLPHSISRTVLGGSPRQCFICSKEAKVCGRLR
                     KHSVEEMQTAISKLLHSFFNKDNQSSSSDKTG"
     gene            25954..27348
                     /locus_tag="ACGEUI_000865"
     CDS             25954..27348
                     /locus_tag="ACGEUI_000865"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HFF8165710.1"
                     /translation="MTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALE
                     MWGGATFDSCLRFLNEDPWERLRAIRKAVKKTKLQMLLRGQNLLGYRNYADDVVRSFI
                     QKSIENGIDIVRIFDALNDPRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDYFVELA
                     KAYQAIGADSICIKDMAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKV
                     AEAGADIIDTAISSFSGGTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDH
                     YREIGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLTEQGLTDKYEEVLAEVPKVRADL
                     GYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKII
                     GDEAVITCRPADLIEPQMIYLRDEIAPYAHSEEDVLSYASFPQQARDFLGRREDPFYD
                     VPVQEVTVQLDIQD"
     gene            complement(27458..28510)
                     /gene="citC"
                     /locus_tag="ACGEUI_000866"
     CDS             complement(27458..28510)
                     /gene="citC"
                     /locus_tag="ACGEUI_000866"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFF8165711.1"
                     /translation="MPYYTISKVFPSDKTTMASVKNLLHQEGIRLDAHLDYTCAIMNA
                     QNDVIATGSYFGNSLRCLCVSSAYQGEGLLNRIVSHLIDEEYALGNYHLFVYTKTSSA
                     AFFKDLGFTEIVHIDNHISFLENKKTGFQDYLMTLNKPEQTPGKVAAIVINANPFTLG
                     HQFLVEKAARENDWVHLFMVSEDRSLIPFSVRKRLIQEGLAHLDNVIYHETGPYLISQ
                     ATFPAYFQKEDNDVIKSQALLDTAIFLKIAQTLQITKRYVGEEPTSRVTAIYNEIMAE
                     QLQQAGILLDILPRKAINQQQDPISASTARQALKDNDWDLLAKLLPKTSLDYFCSLEA
                     QPIIKKIQATSSVKHY"
     gene            complement(28599..29081)
                     /locus_tag="ACGEUI_000867"
     CDS             complement(28599..29081)
                     /locus_tag="ACGEUI_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HFF8165712.1"
                     /translation="MPKRIRKALGVVGKLMSIVPDTTEIIGKTIDNSRPIIEKRMEQK
                     HEKEMQLRTINDVINLPVDQAQAHLEQLGFVVATIPAKPHKKWLHSNLNEVVAMSPKS
                     GKYKIGSLIKLYYITVDVLEKSQDLLDQENLRTVERNQKIADTFESVKHIRFPFKKWR
                     "
BASE COUNT         9192 a         5580 c         5948 g         8705 t
ORIGIN      
        1 ttaataactg tgttgtcact ttgttgaaat gttttcgtat taatgttttt ttatctattg
       61 cgatagataa ttttacttca atttttgatt gaaaatacta aaataagtga tgtggtcact
      121 ttgacaacca tttattggtc cttttagata agacaatttt gtggctcttc agttgaaagg
      181 atgtgttgtt gatgttagag gaaacaatca tgttaaatta actcaggctt aaagaccaaa
      241 aaaggagtga agtctattat gaaaaaaaga aattttaaac ttacagaaat gttacaattg
      301 gttttattag tggttcctgc tgtaaaactt gctaaaaaaa tgtttaacga tagtaaaaca
      361 ccgtctaaaa gaaagtaggt ctacaatgac taaaaaaaat catggcgttt tttgggggtt
      421 gttgcttgga tcagcagcag ctagcattgc ttacttaagt ttatcgtctt ccaaaaaaga
      481 tcaactcttg aaagatagtg ctaaaaaaat agatgatctc aacgcttact tgcaagataa
      541 aagtaagcaa gttctggatg cagtatcgga aaaagtccaa gaatccaaag atgctgttga
      601 agtttacggt ggtatcgccg cagaaacagt tgaagaatct ttgggacagg ctaaagaaaa
      661 ggtagaagga attggcgaag ctactagcca aactatccaa tctaaaatgg aaaaactcac
      721 tagcgataag acagatgctg atgatgaaaa ataggtttta aaataccttt aagataagac
      781 aaaaagtgcc attaggcact tttttaatct tctgttggaa caataatcga aacggctttt
      841 ggaatgatat ctaattgaat gggcagatag tcacttttat ccccatcaat cctggtacgt
      901 ggtaatatcg cttgtttttt agcttgtggt agtaagctaa gcgtattggc ttgaaaatga
      961 ctgatttcct ctgctttgga aaaatcaccc tgtttaaaat cattaatatg ccataaaaat
     1021 ttaaagaaag agactttttt catggtataa acttggaaat agccatcgtc agcttgagca
     1081 ccaggagaaa aagaaggaaa accagcaatg gtatttgtca tcgtgattaa gagaaacttc
     1141 gttttcaggt gaatacgatg gttgtggcta attaaagaga tggcatagct gcgatttcgt
     1201 ttcagtattc gaatactatc ttttaaaaat gctagaggac caaagcgtcg tttttcctca
     1261 gccgttacgt cggcagcaat atcagccagt aatcctaaag ttaaactgga aatcatgtat
     1321 tgctggttag ctttgcaaat atcaacttgc ttgatttgtc cagtcaagat agtatccaac
     1381 gcctcagtaa tttgtaaagg gatatgcata gccttggcaa aattattgac tgtccctgag
     1441 ggcactaagc ctattaaaca atgagctcct ccttcataga tgccaccgat gattttattt
     1501 agtgtcccat cgccaccaag agggataacc aagtcaatct tgtccttagc agcttgtttt
     1561 gctaattgaa aagcctggtc agcatcttta ggggtaataa cttttacaga atcttcggaa
     1621 aaaccatgtt ggcaaaaata gtctttgact tgcctagcaa gttggctttc tttctttcca
     1681 gaattaggat tgtaaaaaat ccgaacggtt ttcatcaggt ctctcctttg acagcttatt
     1741 acttgctatc cattatacca cttctaagaa ttgatgtctt acatcaaatc atcacattaa
     1801 gcctctcaat cagtctgagc atctatttgt cttaaagcat aaagaaagtg atagcgataa
     1861 attggagggc agaggccaat aaaataaaga gatgccaaat catgtggaag tagggtcttt
     1921 ttttggcata aaataccgct ccaatggtat aagacaagcc accaaataac atgagaagac
     1981 caaaagcaag actagttttt tgaatgatga ctggcaagat aaacacaacg agccagccca
     2041 taacgatata aagcatcaaa ctgaattttt cattgatacg tttggcaaaa attttgtata
     2101 aaatcccaaa aagcgtaatg ccccactgca aaacaataat gatatagcct agccaaccag
     2161 aaacaaggga taaggcaaca ggtgtataac ttcctgcgat agcaatataa atcatactgt
     2221 gatcaataat gcgtagaatg tatttgtgaa ccgaaccata agccatggag tggtaaatcg
     2281 tagaggacaa aaacatcaaa aagagactga tgacaaagat ggagatacca atagcggctt
     2341 taaggtcata agtttgataa gcatagctgg ctgaaatagg aagtagaatg agcatagcaa
     2401 atgctccgat agcgtgtgtc acgctgtttg ctacttcctc actaaaagat aagggtaaac
     2461 tttgtttaaa ggtttggttc attgggtcac ctcctgtaat aaataaatag ttttgtggta
     2521 atctttgacc gtttgacagg caacctgcac aatcggtgga atgtcctcct caatgggagc
     2581 cttctgattc caacaggtta caaaagtatt gtaaagactt tcagaatcag cctgtgcctg
     2641 aagttcctcc aatacgcgac taatatcttg aataggaaat accgttttaa acaaactgat
     2701 ggcaattaag cgcgctagtt gttgcttttg gtattttttc ttgataggtt tagtcacata
     2761 cccatgcttg acgtaattat taatcataga tgccgttagt gatttattat ctgacacttc
     2821 tgaaaaatca gtgcactggt tgacataaag taacacttga tccaaatata agtctaaatc
     2881 agggaggtcc ttccaataag gtaatgatgg aaaaatcata gtttcgtctc tctatctagt
     2941 tttgataact agattatatg attaatgaaa acttattgca agcaaaaact gtaaagactt
     3001 aaaagaggaa agtagaaatg gcgaagtgaa gctaatcatg ctataatgtt aaagaattga
     3061 ttgggataga tgccttctgt cgcaatcaga ctattttatc acgaagaaga tgatgctacg
     3121 aaaggaatga tacactcatg gcaatgattc aatggtttcc tgggcacatg tccaaagcca
     3181 gaagacaggt tcaggaaaat gtaaagcatg ttgactttgt aactatttta gtggatgcac
     3241 gcttaccact ttctagtcaa aatcccatgc ttacaaaaat tgttggtgat aaacctaaat
     3301 taatgatttt aaataaagct gacttggccg atgccactcg tacgaaagag tggaaggctt
     3361 attatgaaag tcaaggtatt aaaacattag ccatcaattc caaagaacaa tcaacggtta
     3421 aaaaagtaac agaagcagcc aaggaactca tggctgataa aattcaacgc ttgcgagagc
     3481 gcggcattca aaaagaaacc ttgcgaacca tgattattgg cattccaaat gctggtaaat
     3541 ctaccttgat gaatcgcttg gctggtaaga aaattgctgt agttggcaat aaacctggtg
     3601 tgactaaagg acaacaatgg ttgaagtcca ataaggaatt ggaaattcta gacactcctg
     3661 gtattttatg gccaaaattt gaggacgaat tggtgggctt aaaattagct ttaacaggtg
     3721 ctatcaagga ccaattacta ccaatggatg aagtgactat ctttggcttg aattacttta
     3781 gggaatacta ccctaatcgt ttaaccaaac gttttaagaa tattcctctt gaggaagaag
     3841 ctcctgaaat cattatgacc ttgacacgcc aacttggatt taaagacgat tatgaccgtt
     3901 tttacacttt attcgttaag gaagttcgtg atggtaaatt aggtcaatac accttagatc
     3961 aggtgggaga tatggatgcc gactagtatt aaagctatta aagaaagctt agaggccgtt
     4021 actagcctct tggaccccct ctttcaagaa ttggcaaccg acactaggtc aggcgtccaa
     4081 aaagctctaa aaagccgaca aaaggttatt caggccgagt tagcagaaga agaacgatta
     4141 gaagccatgc tttcttatga aaaagctctt tataaaaaag gttataaagc cattgcaggt
     4201 attgatgagg tgggacgtgg tcccttagca ggtcccgttg tggcagcttg tgtgatttta
     4261 cctaagtatt gtaaaattaa aggccttaat gattctaaaa aaatccctaa agctaagcat
     4321 gagaccattt atcaggcagt gaaagaaaag gctttggcta tcggtatcgg tattattgac
     4381 aatcagctta ttgatgaggt caatatttat gaagcaacca aactggccat gctagaagcc
     4441 attaaacagt tggagggcca actcacacaa ccagattatc tcttgattga tgccatgaca
     4501 ttggatattg ctatttcgca gcagtctatt cttaaaggcg atgccaattc cttgtctatt
     4561 gcagcagcat caattgtagc taaggtcacc agagatcaga tgatggctaa ctatgatcgc
     4621 atttttcctg gttatggctt tgctaaaaat gcaggctatg gcaccaaaga acatttacag
     4681 ggattaaaag cttacggcat aacgcctatc catcgtaaaa gttttgaacc tgttaaatcc
     4741 atgtgctgcg attcaactaa tccttaaaaa gttctgtttt tagcagaatt tttttctttt
     4801 ttacgaataa atgaagtaag gaggtaactt agtgaatcat tttgaacttt ataagctaaa
     4861 gaaagctgga ctgactaata aaaacattct caatattctt gactaccaaa aacaccagga
     4921 aaaatcgttg tcacttcgag atatggccgt tgtttctggt tgtaagcatc cgtctcactt
     4981 tatagaagcc tataagcagt tagatattca aaatttaaaa atggaattta aacaatttcc
     5041 tagtatttct atcttagata agcattaccc aatggcattg aaagaaatat acaatccacc
     5101 tgtcctcttg ttttttcagg gaaatttaga ccttcttgag aaacctaaat tagccattgt
     5161 cggctccaga cgctcaagcg ataccggagt aaagtctgtc cgtaaaattc ttaaagaact
     5221 cgggaaccgt tttgtgattg ttagcggact tgctcgaggt atcgacacta gtgctcattt
     5281 agcctgcctt aaaaatgggg gacaaactat cgctattatt ggaacaggat tggatcgctt
     5341 ttaccctaaa gaaaataggg agttgcaaac tttcttaggg aaaaatcatc ttgtgctaac
     5401 agaatacggt ccaggagaag aagctttatc ttatcacttt ccagaacgga atcgtattat
     5461 cgcaggcctt agccgaggta ttcttgtcgt tgaagcaaaa aatcgttcag gttccttgat
     5521 tacttgtcaa attgggatag aagaaggccg agacattttt gctgtcccag gaaacatttt
     5581 ggacgggaaa tccgaaggtt gccttcagtt aattaaagag ggagcaacat gcgtcacatc
     5641 gggcatggat atcctttcag agtactaaaa aagttaaagt ttcttcctat atagcatttt
     5701 tttattgaca gtttcttaaa aatgggatat tatgctataa gctttaaaca aactgttata
     5761 ggaagtgtgt cgaattggga acaaaaacta caactaagcc aaaaacgggt actaaaaagt
     5821 ccaccactaa aaagaaatca acagcgaaaa aaaatctcgt tattgttgaa tcacctgcca
     5881 aagctaagac tattgaaaaa tatcttggac gcagttataa agttgttgct tcggtaggac
     5941 atattcgtga cttaaaaaaa tcgtctatgt ctattgattt tgataataat tatgagccac
     6001 aatacattaa tatccgtggt aagggacccc ttatcaattc tttaaaaaaa gaagccaaag
     6061 ctgctaaaaa agtttattta gctagtgacc cggatcgtga gggagaggct atttcttggc
     6121 atttgtcaca tatcttagga cttgatcctc aagataataa ccgagtggtt tttaatgaaa
     6181 ttacgaaaga tgcagttaaa catgcctttg tggagcctcg tcagattgat atggacttgg
     6241 tagattctca acaagcacgt cgtgttttgg accgtattgt gggatattcc atttcaccaa
     6301 ttctttggaa aaaggtcaaa aaaggacttt cagcaggacg tgtccagtcg gttgctttaa
     6361 aactcatcat tgaccgtgaa aatgacatca aagcatttgt tccaaaggaa tactggtcta
     6421 ttgatggtct ttttaaaaaa ggaactaaaa agtttcaagc gactttttac ggtattaatg
     6481 gcaaaaaaac aaagcttgac aataacaacg atgtcaaaga ggtattagca aagctaacaa
     6541 atgaagattt tcttgtcagc aaggttgaca agaaagagcg acgtcgtaat gctcccttac
     6601 cttacacaac atcatctctt caacaagatg ctgctaataa aatcaatttc agaacgcgta
     6661 aaaccatgat ggtagcccaa caactgtatg aggggattca tttaggagaa aatggaactc
     6721 aaggtttaat tacctatatg cgtacggatt ctactcgtat cagtccagta gcacaaaacg
     6781 atgctgctca atttattatc aatcgctttg gagctaatta ttcaaaacat ggtaatcgtg
     6841 tcaaaaatac cagtggtgtc caagatgctc acgaggcgat tcgtccatct agtgttaatc
     6901 atacgccaga ctctattgcc aaatacctta acaaggatca gttaaaactt tatacgctta
     6961 tttggaatcg ttttgtggct agccaaatga cagcagctgt ttttgatacc gtaaaggtga
     7021 acttggagca aaatggcgtg atttttgttg ccaatggtag ccaaatgaag tttgatggtt
     7081 acatggctgt ttacaatgat tccgataaaa acaaaatgtt acctgagatg gcagaaggag
     7141 aaacggttaa aaaaatatcg acctctccag aacagcactt tacgcagcca ccagctcgtt
     7201 attctgaagc aactctaatt aaaacgctag aagaaaatgg tgttggacgt ccatcaactt
     7261 acgctccaac cttagaagtc attcaacgca gatactatgt taaattgtct gctaagcgct
     7321 ttgagccaac tgagcttggg gaaattgtca acaagcttat tgtcgaattt ttcccagata
     7381 ttgtcgatgt tgcctttact gccgagatgg aaggtaagct agatcaagtt gaaatcggag
     7441 aagaacaatg gcaacatgtt atcgatcaat tctatcaacc ctttgtcaaa gaattgaata
     7501 aggctgagtc tgaaattgaa aagattcaaa tcaaagatga accagctggc ttcgactgtg
     7561 atgtttgtgg tcacccaatg gtcattaagt taggacgttt tgggaaattt tacgcgtgta
     7621 gcaacttccc agagtgccgt aatacaaaag ccatcactaa agaaattggt gttacttgtc
     7681 cggtttgtca taaaggacaa gttatcgagc gtaaaaccaa aaaaaatagg atattttatg
     7741 gttgtgatca gtatccagat tgtgagttta tctcgtggga tttacctatt ggacgtgctt
     7801 gccctaagtc aggtgactat cttattgaga aaaaagttcg tggtggcaaa caagtaatgt
     7861 gtagcaatga gacctgtgat tacaaggaag aaaaaattaa ataaaaaaag aattggaact
     7921 ccttgtcttg ttccaattct ttttttattt acaaaggtat ttttaactac aaaaatcgct
     7981 agttcgtttt taaaaggctt aaaaattcct caataactgg actgaaactc gcatcatctc
     8041 ggtaagcaat cccaataggg taaaaattat ttttatcttg caaaggaatc caaactaagt
     8101 tatctacctg attaagactc tcaaaatcac tcgggaggat agtcaaggta tttaaatcgt
     8161 gcaaactatg aataagaact tcccaatcat tgtgtttaaa gacaatatct ggttcaaaac
     8221 cgagagctcg gcattttcta ggaagcattt cgcctaacat gtagtgatcg tttaaagaag
     8281 caatcttata gccttttaaa tctttaagtt ttaactgagg atgttgcgcc aaagggtgct
     8341 gtttgaggag aacgatactg accttatagc cttttgtgga cgtttgtaat agctcaatgg
     8401 taatatcctt tcgaatactt agaaaggaga ggagaccaat atcgattttt ccttgggaca
     8461 ataattctta taatttgcga gaaccatcct gtataagcga taattcaaca tttgagtgag
     8521 tagccataaa gattgaaatt tgtttcatga attgtatagc aaacagtgat gttaattcaa
     8581 cacgaatcat ctcttttttt cttgatttaa ttgctgaata tccgcaacaa tttgattaaa
     8641 gtcatccaca aggtctttgc ctttgtgata aaatatctcc agcttcggtt agtagtagct
     8701 gtcaatcact ttgtgtaaaa agcgtcgtgt tcaaactagg actctagcct tttaattgat
     8761 tgagaaagag taggttgcgt tacaaacaaa cttttagcag cgtgggaatg attttttgtt
     8821 tcagcaactg caataaaata ggttaattgt ctaatatcca tagtagatcc ttcatgattg
     8881 gtcaaggaat agcgtctcta cttggtttac aacctatttt gatgtctctc cttattgcca
     8941 tgattttctg cttccttatt gtttctccaa ttacaacggt aggaattgct cttgcgatta
     9001 acttatctgg tattggttca ggtgccgcaa gctttggact ttgcttagca ggttgggctg
     9061 ttaattcaaa aggaacttca cttgctcacg tcttgagatc accgaaaatt tcaatggcca
     9121 acgttttatc aaaaccaaaa atcatgttgc caatgttatg ttctgcagct gtacttggtg
     9181 ttattggtgc tatctttaat attcaaggaa cacctgctag tgcaggcttt ggaattagtg
     9241 gtttgatcgg acctatcaat gcccttaatt tagctaaagg cggatggtgt ccagtaaata
     9301 ttctacttat cattatcatt tttgtagggg caccaattgt tctcaatatg atctttaact
     9361 acctttttat caaggtgctt aaagtgattg atccaatgga ttacaaactt gatatctaag
     9421 aaaggaactt ttgatgaaat taaaattata taatgttaga ggcgaagaag cagtcttagc
     9481 aaaaaaatgg gctgatgaca atgggattga gatttccttg acggagtctc ccttaactcc
     9541 tgaaacggtt aaagaagctg aaggctttga cgggattgcc aatgcccaaa ttggtccttt
     9601 agatgatgcg atttatcctc tcttaaagga aatggggatt aaacaaatcg ctcagcacag
     9661 tgctagtgtc gatatgtata accttgattt ggccaccgaa aatgacatta ttatcacaaa
     9721 tgttcctagc tattctccag aatccattgc agaatttacg gttactattg tccttaattt
     9781 aattcgtcat gtggagttga ttcgtgaaaa tgttaaaaaa caaaatttca cttggggact
     9841 ccctatccgc ggccgtgttt taggtgatat gacagttgcg attatcggaa ctggacgtat
     9901 tggtctagct actgctaaaa tctttaaagg ttttggctgc aaggtcgttg gatatgatat
     9961 ttaccaaagc gatgctgcca aagctgtctt ggactataag gaatctgtag aagaagcgat
    10021 taaagatgct gatcttgttt ccttacacat gccaccaact gcagagaaca cacacctttt
    10081 caattctgat ttatttaaat cattcaaaaa aggggctatt ttgatgaata tggcgcgtgg
    10141 tgccgtcatt gagacgcaag atttactgga tgctttggat gcaggcttac tgagcggagc
    10201 tggtattgac acttacgaat ttgaaggacc ttatatacct aagaattttg aaggtcaaga
    10261 aattacagat tcactattta aagctttgat taaccatcct aaggttattt atacacctca
    10321 tgcagcttat tatactgatg aagccgttaa aaacctagtt gaaggtgccc ttaatgctac
    10381 agtagagatt atcaaaactg ggacaacgac aactcgtgtt aactaacaac tgatttgacg
    10441 tccattataa aggaataatt ctaaaaattc ctttataatg gacttttttg tattagtccg
    10501 ttataataga ctgtaagaaa ggggactctt atgacatata tagcgcttat tggagatatt
    10561 atccagtcca aacaattgac ggatcgttcc aaagttcaaa aaactttagc agcttattta
    10621 gacgacctca acaaaacctt tgctccttat attatttcta aattatccct aacgttggga
    10681 gatgaatttc aaggactttt tcaggtggat actcctattt tccatctgat tgatttgatt
    10741 aatcatcaca tggatatccc tattcgattt ggagtaggag taggttctat tctaacagac
    10801 atcaatcctg atattagtat tggggctgat ggacctgctt attggcatgc tcgtgaggct
    10861 attcgttata ttcatcaaaa aaatgattat ggtaatacta cattagctct tcggactggt
    10921 catcacaacc aggatgatgt attaaacagt ttactagcag ctggagatgc tattaaagcc
    10981 aactggcgag ctagtcaatg ggaaattttt gacacgcttt tagatttggg catttatgag
    11041 gaatactttg atcaacaacg actaggaaag caattatctc ttagctctag cgctttgtca
    11101 aaacgtctaa aaagtagtca tgttaaaatt tatctgagaa cacggcagag tgcgctaaac
    11161 tgtctcaaac aaatcaagga ggaaactgat gattaatggt gtctctcact acctagctca
    11221 aacccctaca cttaccctgt ttctcatttg tcatgtgtta tcagactacc agttacaaag
    11281 tcagcaagta gctgatttaa aagaaaaaca tttgacttat ttaggttacc acttgattgg
    11341 agttagcatt cccctaattt gtttaaccct cattatccca caagcatggt tgatgagcct
    11401 attggttatg ataagtcacg cgcttattga ctggctaaag ccaaagatgg ctaactctct
    11461 aaaatggaaa agagagtgga tttttctgct ggatcaatgc ttacatatcg ctattagttc
    11521 atttgctggg ctaaggttag caggggtgac cttgcctaat tggcttccga tatctatttt
    11581 gatgaccgtt ttatttatcc tactcattac caaaccaact aatattgtct ttaaactctt
    11641 tttcagcaaa taccagcctg accaaggaga aaaaatggac acgatcatag gagcgggggc
    11701 taccatagga attctagaga gaattgttat tggggtgtgt atgatcatgg ggcaatttgc
    11761 ttcaataggt ctggtcttta ccgctaaatc tattgctcgc tataataaaa tttcggaaag
    11821 tccagctttt gcagaatact acctaattgg ttctttattt agtattctca gtgtttttat
    11881 cgcagcatgg atttgtttct tctagttgaa aagggttccc gtagaaagag actaacgctt
    11941 agtctctttc tttttctttc ttcttatttc tgttataata agtcaggtaa ctacattaga
    12001 aagatttgtg ggtctgtcat ctaagagatg tcattcccga agaggtatta gtgacttgtc
    12061 tcaatcaact gcaacttata ttaatgttat tggagctggg ctagctggtt ctgaagctgc
    12121 ctatcagatt gctaagcgcg gtatccccgt taaattgtat gaaatgcgtg gtgtcaaagc
    12181 aacaccgcaa cataaaacca ctaattttgc cgaattggtc tgttccaact catttcgtgg
    12241 tgatagctta accaatgcag tcggtcttct caaagaagaa atgcggcgat tagactccat
    12301 tattatgcgt aatggtgaag ctaaccgcgt acctgctggg ggagcaatgg ctgttgaccg
    12361 tgaggggtat gcagagagtg tcactgcaga gttggaaaat catcctctca ttgaggtcat
    12421 tcgtggtgaa attacagaaa tccctgacga tgctatcacg gttatcgcga cgggaccgct
    12481 gacttcggat gccctggcag aaaaaattca cgcgctaaat ggtggcgacg gattctattt
    12541 ttacgatgca gcagcgccta tcattgataa atctaccatt gatatgagca aggtttacct
    12601 taaatctcgc tacgataaag gcgaagctgc ttacctcaac tgccctatga ccaaagaaga
    12661 attcatggct ttccatgaag ctctgacaac cgcagaagaa gccccgctga atgcctttga
    12721 aaaagaaaag tattttgaag gctgtatgcc gattgaagtt atggctaaac gtggcattaa
    12781 aaccatgctt tatggaccta tgaaacccgt tggattggaa tatccagatg actatacagg
    12841 tcctcgcgat ggagaattta aaacgccata tgccgtcgtg caattgcgtc aagataatgc
    12901 agctggaagc ctttataata tcgttggttt ccaaacccat ctcaaatggg gtgagcaaaa
    12961 acgcgttttc caaatgattc cagggcttga aaatgctgag tttgtccgct acggcgtcat
    13021 gcatcgcaat tcctatatgg attcaccaaa tcttttaacc gaaaccttcc aatctcggag
    13081 caatccaaac cttttctttg caggtcagat gactggagtt gaaggttatg tcgaatcagc
    13141 tgcttcaggt ttagtagcag gaatcaatgc tgctcgtttg ttcaaaagag aagaagcact
    13201 tatttttcct cagacaacag ctattgggag tttgcctcat tatgtgactc atgccgacag
    13261 taagcatttc caaccaatga acgtcaactt tggcatcatc aaagagttag aaggcccacg
    13321 cattcgtgac aaaaaagaac gttatgaagc tattgctagt cgtgctttgg cagatttaga
    13381 cacctgctta gcgtcgcttt aacactaaaa aagagttcct tttcagttga gttgctgaag
    13441 aggacttttt tacttgctga tttgagcgct agctatgcaa aagaatgcac aaaaaataca
    13501 tgaatagact atggtcacta gaagcaatat tatataatga atagtactca ttagttgatt
    13561 cagtttggag gttttagcat gcagcagcaa gtcgcaataa cagaaacagt tttacgggac
    13621 ggtcatcaaa gtttgatggc aacgcgttta tctattgaag atatgcttcc tgtccttacc
    13681 atcttagaca agattggtta ttattcccta gagtgttggg gaggagcaac ctttgatgcc
    13741 tgtattcgtt ttttgaacga agacccttgg gaaaggctgc gcacgttaaa aaaaggctta
    13801 cctaataccc gtttacaaat gttattaaga ggccaaaacc ttttaggcta ccgtcattat
    13861 gcagatgaca tcgttgacaa attcattagt ttatcagctc aaaatgggat tgatgttttc
    13921 cgcatttttg atgccctaaa tgacccacga aatatccagc aagccttaag agctgtgaaa
    13981 aagacgggta aagaggctca actgtgtatt gcctatacaa ctagcccagt gcacacgctt
    14041 aactattacc tttctttagt gaaagaattg gttgagatgg gagctgattc tatctgcatc
    14101 aaagatatgg ctggtattct aactcccaaa gcggctaaag agttggtatc tggtatcaaa
    14161 gccatgacta acttacctct tattgtgcat acccatgcaa ctagtggcat ttctcagatg
    14221 acctatctag cagcagtaga agctggtgct gatagaattg atacggcttt gtcacctttt
    14281 agcgaaggca cttcacaacc tgctacagaa tccatgtatt tagccttaaa agaagcaagt
    14341 tatgatatca cattagatga aacgctactg gaacaggctg ctaatcacct tcgacaagcc
    14401 cgtcaaaagt acttagcaga tggcattctg gatcccagtc ttctctttcc agacccaagg
    14461 acactacaat accaagttcc tggaggcatg ttgtctaata tgctatctca gctgaaacaa
    14521 gccaatgccg aatcaaaatt agaagaagtt ttagcagaag ttcctcgggt aagaaaagac
    14581 ttgggttatc ctcccttagt aacaccgctt agccaaatgg tcgggactca agctgctatg
    14641 aatgtgattt taggaaaacc ttaccagatg gtgtctaagg aaattaaaca atacttggca
    14701 ggcgactatg gcaaaacacc agcaccagtc aacgaagact taaaacgaag tcaaattggc
    14761 tcagctcctg tcaccacaaa tcgtcctgca gatcaactaa gtcctgaatt cgaggtgtta
    14821 aaagcagaag tagcagattt agcccaaacg gacgaagatg tgctgactta tgccctcttc
    14881 ccaagcgtag caaaaccgtt tttaacaacc aaataccaaa ctgatgacgt cattaaagtg
    14941 actgccttta tcaaagcata ataggctatc ttaatcagat agaaacaaaa atatggagat
    15001 caaatgacct attcctttat tgatatctta caagttacct tagtatcctt gttcttagtg
    15061 ttaacctgtt taacagggat tatgggatta atgatgctaa gtggtaaatt attgtcacaa
    15121 gaaaaaaaaa ctctctctca acaagcttcc agcattcctc aagagcaaga agctgacaag
    15181 ttgagtggag aagctctcga aactctttta gccaaagacg aattggctag aattgctgtt
    15241 atgactagtc ttgcttatgc tagccaagaa gagtcaggaa aacggtttga agttattgcc
    15301 gtggaaagga aatcctaatg aaaagatatg aagtgactgt caatggacag gtttatgaag
    15361 tgagtttacg tgaactagtt gatggagaaa ctgttgaagt tagccagcca gcagcacccg
    15421 ctactgagaa agaaatgaat gccaacgctg ctggcggtgg cattcaggta aaggctccaa
    15481 tgtctggaac agtcttgtct atctttgcta cagaaggaaa agctgttaaa aaaggagaag
    15541 ctgttctggt cttagaggcc atgaaaatgg agaatgaaat tctagcacct gcagatggtc
    15601 tggtttcaaa aattcatgtt gtggctaacc aaacggtcga gtctgaacaa gttttgattt
    15661 cattctagga ggaaaccatg ctagacgtac tcaatcaaat ggtccaatcg tcaggattgg
    15721 ctcatctaac tgttaataat cttatcatga tttgcttggc cagtttcttt ttataccttg
    15781 gtatcaaaaa ggaatatgag ccttacctta tggtaccaat cgcctttggg attttactcg
    15841 ttaatttgcc tatggcggga ttgatggacc accctgccaa tggtaatcct ggtggccttc
    15901 tttattacct atataaggga actagcctag ggatttaccc acctttaatt tttctttgtt
    15961 tgggggcatc aactgacttt ggtcccttga ttgccaatcc taaaaccatt ttactaggag
    16021 gcgcagcaca agttgggatt ttcttagcct ttttccttgc tattatgtta ggcatgacac
    16081 ctcaagaagc ggcttctgtc gggattattg gtggtgccga tggaccaaca gctatttatg
    16141 tgacgacaaa gttggcgcct gatttactct ctactatcgc cctagcagct tattcttaca
    16201 tggctctggt acctattatt caacctccca tcattaaact cttgactacc aaggcggaac
    16261 gccaagtgaa aatgactcaa gcaagaactg tttcacaaaa agaaaaaatc attttcccga
    16321 ttatggtaac aatctttgtc agccttttgg ttccaagtgc tacaaccttg gttggctgtt
    16381 tgatgctggg aaatctggtc agagaaatta aaattgttcc taaaattgta gagaatctcc
    16441 aacaagtggt tatgttttgc attactatca ttttagggtt aaccgttggt gctaaggcca
    16501 atggagatct ctttttatca gccacaacct tgaaaattat tgcccttgga ctaatcgcct
    16561 ttgctgctgg gacagcaggg ggtgtcttga tggggaaggt catgtactat ttatctggta
    16621 acaaagtgaa ccctatgata ggagcagcag gtgtttcagc tgttccgatg gcagcacgag
    16681 tggtccaaaa gatcggacaa gaagaagatc caagtaattt cttactcatg cacgccatgg
    16741 ggcccaatgt agctggcgtt attggctcag ccattgcctc aggtgccttg ctggccttct
    16801 ttggctaatt gtgaaaaagg attagtaaat taatcttaat aaatcattat caggagttgg
    16861 aagcaatcta gctccttttt tagtagaaag ctcgagtgat aaactattcc aaaataatta
    16921 taaaaaagac tgtcctgatt gtacggacta tatcagttga cttttatagt ctagctgaaa
    16981 tagcagtgca aaactaagac actcgtttta aagtagtttt tctaaaaaag caaaataaaa
    17041 ggtcagagcc aataaatctg ccgcgccccc agggcttaaa tgctgattaa tgaggcattg
    17101 attgtaggag gacaaagcca atcttaggtc agttgttgat aaatcttgtt gcaggagtag
    17161 tcgcatatct gccttaacgc ctttccatgc ttcaatccct ccacgatgaa tcagatttcc
    17221 atcttcaaca atagacatca aataaacaag aagacgaagc tgactggttt cggggtcatt
    17281 ttgacttatc atctgacgaa agtagggcaa ggcatggtct gttaaggtcg tgaatccctc
    17341 gcttgcctca ccccgaggtc ctttgatgcc ataagttaca aagagttgct ccccataagt
    17401 gaactctttt ttagtgttca aatgaccaag gtctgtttga atcaaatgtg ccgtcatagg
    17461 ttttactaaa cgacagatag ctagagtatc ttccttgcta aaacgcccta aatcagtcat
    17521 taagtgaggt gttcttgcta gataggcccc agttgcacca agaagtaaag ccatggaaaa
    17581 attcaggcct ttatgagtgt taattccctg agtagcacaa aacatggttt cctcggcctt
    17641 ttgacctagt tgtctaagac ggttaaacag aagaagtggc tcttcttttg cataagcaaa
    17701 acctgtttcg atataagcct gaaaaaaagg agagagtgct atcatactgt cgataaaggt
    17761 tataaaggac atgtcatcat gggcaccgtt atcaaatcga tcaactaaac caggttttgg
    17821 agatagtgag acttcataaa gcaaagcttt taaggccagt tgactaatac ttgttagaac
    17881 tgccttagtc attttgctca ggcctcattt cttttaagat aaattcaagt gagctattca
    17941 agtgttcatg tgtaatcaac gtaatttgct catgatcttt gttcttcata cctcggtaaa
    18001 tcatccagtg ttcagataga gctcgtttac ggcgttcaac tgaagaaatc gaaatttttc
    18061 taaagtagac caaataagct tgcaattctg tcacaatttc ttttaagcgg accatttggc
    18121 tatagctgta aatcaaattg ttaaaggcat taaaattatc caaaatacga ttggtgtcat
    18181 cctctgcgat aaaggtttca cagtcttcca acaagttatg catgacttta aaatcttctt
    18241 ctgtcattaa atgcatcgct tgcactgttg cgagagtctc tagagctttt cgaatttcaa
    18301 aaatttcaca ggcatccttg atactaactc ctttgacaat aatgcctttt ttaggaatgt
    18361 gctctacaag gtgttcttca gacaataatc ccaaagcgta gcgaataggt gtcctgctaa
    18421 tattaagcgc aatggaaaac tctttttcat taatgcggct tccaacagga atttgtcgca
    18481 aaataatggt ctttttaaga gcgttataaa aggctatttt caaagggata tttcttgata
    18541 ggtcaagatt ttgtttaacg gcttcgataa taggattcat gtcatctcct taatgctagt
    18601 gtcctcactt tattatactt aaatttgtga aacattccaa taggactaga tgagttctga
    18661 aaaattaaag ccatgctcac aaaaggacat gactttaatc atctcataag gaataaggta
    18721 gtagtgttat cgttggttta ttgtggaatg taaagtggca tttgtcccat ggcaataatg
    18781 gttaccacaa agataacaaa gatgataagg gcatatttag cagcctcttt ttgccattcc
    18841 cccatgtcaa gacctgtcaa gcgaagaaga agataaatga aggcaactaa tggactaagt
    18901 aagtggaagg cttgtcccat gagggaagca agtgccatag ccatgttact gaaaccatat
    18961 tcagcaccag cttctgcaag aacaggcaag ataccatagt aaaaaccatc attggagata
    19021 aagaaggtac caggtgcaga aactaaggca ataacgagac cccagaaacc tgccagttgt
    19081 tttggaataa tggttgcaaa actttgtgca agagcgctag ccataccaga accttgaaaa
    19141 agacccataa agataccagc agcaaagacg agaataacca cttgaaccgc atcaccagca
    19201 ttgtcaccaa tgcgttttga ttgatcttta agcactgggt aattgaccat caaggcgata
    19261 caagttccca ccgcaaaaag aagaagcggt gccatagcaa tagatttgtt gaaagagcca
    19321 gcaactaacc aagtgattaa tccaatggtt aagatagcat tgaaaacaaa gtttttagga
    19381 cgacgaataa aaagggtatc tgggtcagta atgtctgtga gttgacgcat gtcttcttca
    19441 gacaagtcaa taacaccaag tcttgcacgt tctttacgcc ccatgcttgg agcaacccaa
    19501 caaatcacat aaagaagaga taaaatcata cccggtgcaa gatagccaag aatttcagga
    19561 ccgacaccaa gaacagacat cgcacgagca gtagggccac cccatggcag taagttcata
    19621 atagtatttt gaagaataat caagacacct aggttcatga ttttcatgtc caatttttta
    19681 tagataggta agaaagcaga gcaacaaatt aaagtagtgg ttgttccatc accattaaga
    19741 gatactgcag cagcaacgac agctgttgcg ataagcacct tcattggatc acctttagca
    19801 aagcgaatca tcttttcagt aatcggatca aacaatcctg catctaacat gacagagaag
    19861 aacaaaatgg caaatagcaa catgatacct gttttagcag tattgttgat accatacata
    19921 accattggtc cgatagctgt taggtcttta gcaattccac cgtcaccaac aaaggcaaca
    19981 aatttggcat ctgcattgaa gtcagctgaa ccagtcagta taacagcaat cgtcatgatt
    20041 aatggaatca tgaccaaagc agtaaaaggg gtcatttttt tcttcattac cacgtacata
    20101 aagacaataa tcatggcata agccagcatt gttaataaca tcattgttat ctcctttttc
    20161 aagttatgat ttcagtatag atgaaagcgc ttataaaata aatacttctt tgtgcagaaa
    20221 gacacaaaaa aaacaatttg aaaattaatt ttgaaaacca tttttattac atcaggtgac
    20281 tttaaatttt ctataataat gatgaaccta gttagatcaa gcttttcttg ctatcagtat
    20341 cttgatctca gtttttcaac agttacctaa aaataaaata aaaggtaact gttgaaaaac
    20401 tttatgaaac attgaaagcg ctcttagaaa gaacacaaaa aagccatctc aaaaaagatt
    20461 ggtagtatat aataaactta ctagttcctt aaataggtaa atgtgaaaaa gaggtagctc
    20521 atgcagtttt tacaaaaatg gtttaaacac tctaaaaaag agaagttgaa agaatcctcg
    20581 tcccttagta cagaaataga accttctgaa aactgggcaa agattcccgc ttatattcct
    20641 gcagacaaaa gtgattacaa aaaggttact ttaattacca gtgttattgc tgctggcgat
    20701 agacctaata gtcaattcaa ggttaaacgc attctcaagc gcaatcctga ggcaatcact
    20761 gtttctttaa ttgcttcaag tatcgctgca ggtgtttacc cagaaagtca gtttcgggtg
    20821 acatccatct attgcaaaag gtagtcggtg aacgatactt tagacgaact catgacacaa
    20881 taacatatag gagaaaaatg atgttacgaa aatttaaaat taccattgac ggtaaagagt
    20941 atcttgtaga aatggaagaa atcggtgctc ctgcccaagc agctgccccc gctcaaccaa
    21001 tatccactcc agtacctgtc cctactgaag caagcccaca agtggaggaa gcgcaagcac
    21061 cacagccagt agctgcagcg ggagcggatg ccattccatc accaatgcct ggaaccatct
    21121 taaaagtctt agtagcagtg ggagaccaag taactgaaaa tcagccacta ttgattttag
    21181 aagccatgaa gatggaaaat gagattgtgg cctcatcagc aggaaccatt acagctatcc
    21241 acgtcggtcc aggtcaagtg gtcaatcctg gtgatggtct cattacaatt ggttaatagt
    21301 actataacta gaataaatag acagaaaagg aagtgaattt gtggacactt taatcgcagg
    21361 aataacctcc atcaccattc ctcaaattgt tatgatggtc attggtgccc tcttaatgta
    21421 tctaggaatt aaaaaagaat acgaaccaac cttacttgtc cccatgggac ttggaaccat
    21481 tctcgttaac ttccctggtt caggagtttt aacacaagtg gttaacggag tcgagcaaga
    21541 aggggttttt gaggctctct tcaattttgg gattggaaca gaacttttcc cactgttgat
    21601 tttcattggt ataggagcca tgattgactt tggccccttg cttcaaaacc cattcatgct
    21661 cttgtttggt gctgcagctc aattcggaat tttctttgtt gtggttgttg ccgtactggc
    21721 tggctttgac attaaagaag cagcttcaat cggcattatc ggagcagcag acggaccgac
    21781 ttctatcttt gttgccaacc aactggccaa ggatttactg ggtccaatta cggttgcagc
    21841 ctattcttac atggctctgg ttcctattat ccaaccattt gccattaaat tagtgacaac
    21901 gaaaaaagag cgccgtatcc gtatgactta caaagctgag aatgtatctc aaatgactaa
    21961 aatcttattc ccaatcatca ttaccttagt agcagggttc atcgccccaa tttctctacc
    22021 tttagttggc tttttgatgt tcggtaactt attacgggag tgtggtgtgc tcgatcgcct
    22081 gtcacaaact gctcaaaatg aattggtaaa cattattagt atcttgcttg gattgaccat
    22141 ctctattaaa atgcaggcag acctgttctt aaatgtacag acactcttaa tcatcgtctt
    22201 tggtttactt gcttttatca tggattctat cggtggggtc atgtttgcca aatttctcaa
    22261 tctcttccga aaagagaaga ttaatccaat gattggcgct gcgggtattt cagcttttcc
    22321 aatgtcaagt cgtgtcattc aaaaaatggc aacagatgaa gacccgcaaa actttatcct
    22381 catgtatgct gtaggtgcca atgtctctgg ccaaatcgcc tctgttatcg caggtggttt
    22441 gttgctagct tactttagct aagaaaggaa ggaaacctta tgacaattaa tatggaccat
    22501 ttgattagtt cctttgagct aatggcgctt ggtatggcag gtgtctttat cgtgttaggc
    22561 attctctatc ttgtcgcaga gatcctgatc aaacttttcc cagttagtaa ataacaatat
    22621 catttaagaa aatcaaggag gtaaacgcaa tggatattaa acaaactgcc gttgctggtt
    22681 cacttgaatc aagtgaccta atgattacag tatcccctaa tgacgagcaa accattacaa
    22741 taaccctaga cagtagtgtt gaaaaacaat ttggcaatca cattcgtcaa ctcattcatc
    22801 aaaccctagt gaatttgaag gtgacggctg ctaaagtaga ggctgttgat aaaggtgcat
    22861 tagattgcac cattcaagcg agaaccatcg cagctgttca tcgcgctgct ggtattgacc
    22921 aatacgattg gaaggagatt gactcatgga acgtttaaga agaacaatga tgtttgtgcc
    22981 tggtgccaat gcagccatgc ttcgagatgc tcctttattt ggcgctgatt caatcatgtt
    23041 tgacttggaa gattctgttt cactcaaaga aaaagacacc tctagagctc tcgttcattt
    23101 tgcgcttaaa acttttgatt attcaagcgt tgaaacggtt gttcgtgtga atggtcttga
    23161 ttcttgtggg gctttagaca tcgaagctgt tgttttggca ggtgtcaacg tgattcgtct
    23221 tccaaaaaca gagactgctc aagatattat tgatgtggag gctgttattg aacgtgtcga
    23281 acgcgagaac agcattgaag ttggtcgcac acgtatgatg gcagccattg aatcagccga
    23341 aggtgtccta aatgctcgtg agattgccaa agcttctaag cgcttgattg gtattgctct
    23401 tggagcagaa gactatgtca cgaatatgaa aacgcgtcgt tacccagatg gtcaagaatt
    23461 attctttgct cgtagcatga ttttacacgc tgctcgtgct gctggaattg ctgccattga
    23521 tactgtttat tctgatgtca ataataccga agggttccaa aacgaagttc gcatgatcaa
    23581 acagttagga tttgatggta aatcggtcat caaccctcgc caaattcctc tggtcaatga
    23641 gatttatacc ccaacaaaaa aagaaattga ccatgccaaa caagtcatct gggcaattcg
    23701 tgaagctgaa agcaaaggct ctggcgttat ttccttaaat ggaaaaatgg ttgataaacc
    23761 aatcgttgaa cgtgcagaac gtgtgattgc cctagcaaca gcagcaggtg ttttatctga
    23821 ggaggatatt taaagatggt aacaaataaa ctcggtagag atattcctca accatacgcc
    23881 gaccaatacg gtgtctttga aggagaactc gcaaatatca aacagtatga cgaatcaagt
    23941 cgccgtatta aaccagttaa gcctggagat tctaaattac taggatctgt tcgtgaagcg
    24001 attgaaaaaa caggtttaac agatggcatg accatttctt tccaccacca tttccgtgaa
    24061 ggggatttca ttatgaatat ggtcttggag gaaattgcca aaatgggcat taaaaacctg
    24121 tctattgccc caagttccat cgctaatgta catgagcctt tgattgatca catcaaaaat
    24181 ggtgtggtca ctaacatcac ttcatctggt cttcgtgaca aagtaggggc agccatctca
    24241 gaaggactaa tggaaaatcc tgtggttatt cgctcccatg gtggtcgcgc tcgtgccatt
    24301 gctagtgggg atatccatat tgatgttgcc tttctgggcg ccccaagttc agatgcttac
    24361 ggaaatgtta atgggacaaa agggaaagca acctgtggtt ctttgggcta tgccatgatt
    24421 gatgccaaat atgcggatca ggtggttatc ttgacagata atttggttcc ttatcctaat
    24481 accccaatca gcattcctca aacagatgtt gactatgtgg taacagtgga tgctatcgga
    24541 gatcctcaag gaatcgccaa aggggcaact cgttttacga aaaatcctaa agaactctta
    24601 attgcagaat acgccgctaa agtaatcacc aactctcctt attttaaaga aggattctct
    24661 ttccaaactg gaacaggtgg cgcttctttg gcagtaaccc gtttcatgcg tgaagctatg
    24721 attaaagaaa atatcaaagc tagctttgct cttggtggta ttaccaatgc tatggtggag
    24781 ttgctcgaag aagaactggt tgaaaaaatt cttgatgtcc aagactttga tcacccatca
    24841 gctgtttctc ttggtaagca tgctgaacat tatgaaattg atgccaacat gtatgcctca
    24901 cctttgagca agggtgctgt tatcaatcaa ttagacactt gtattttatc agcccttgaa
    24961 gttgatacta acttcaatgt taatgtgatg acaggatctg acggcgtgat tcgtggcgct
    25021 tctggaggac actgcgacac tgcctttgcg gctaagatga gtttggttat ttcaccactt
    25081 atccgcggac gcatcccaac ttttgtagat gaggttaata cggtcattac accaggaaca
    25141 agtgtcgatg tgatcgtcac agaagtggga attgccatta acccaaatcg tcaagactta
    25201 gtagaccatt tcaaatccct caatgtgcca caatttagta ttgaggagct aaaagagaag
    25261 gcttacgcca tcgttggcac acctgagcgt attcaatatg gtgataaggt tgttgctctt
    25321 attgaatacc gtgacggtag cctcatggat gtggtttaca atgtgtaaag acacttattt
    25381 ttcaggcgaa gctatccaac ttagtgatat gttaagagcc cgcgaagaaa gagctctgcg
    25441 tcagctgcat ttattaaagg agtacccaga aggtagctta ttatcggtca ccatgaatat
    25501 ccctggacca attaaaacct ctcctaaact tcttgaagct tttgatatag tgattaaggc
    25561 cattcaaact gccttagctg acgataagat ttgttaccag ttgcgattac tgcctacaac
    25621 gggttatgag tattacctca tcacaagtct acctagccgc gacctgaagt taaaaatgat
    25681 agccttagag acagagttgc caataggtcg tctcatggat ttagatgtct tggtcttgca
    25741 aaatgatctg cctcattcaa ttagcagaac cgtattagga ggctccccta ggcaatgttt
    25801 tatctgttct aaagaggcca aagtctgcgg tcgcctacgt aagcacagtg tcgaggagat
    25861 gcagactgct atttcaaaat tactccattc atttttcaat aaagacaacc aatcatcgtc
    25921 atcagataag acaggttgat gaggagaaca attatgacga aaatccgtat aacggaaaca
    25981 gttttacgtg atggtcaaca aagtcagatt gccacacgta tgacaaccaa ggaaatgatt
    26041 cctattctcg aaacgctaga caatgctggt tatcatgccc ttgaaatgtg gggaggagca
    26101 acctttgatt cctgcctacg ctttttaaat gaagaccctt gggaacggct aagagccatc
    26161 cgtaaagcag tgaaaaagac taaacttcag atgctcttac gcggacaaaa ccttcttgga
    26221 tatcgcaatt atgctgacga tgtggtcaga tcctttattc aaaaatccat tgaaaatggg
    26281 attgatattg tccgtatttt cgatgcttta aacgacccac gcaacttgca aacagctgtt
    26341 tcagcgacta aaaaatttgg agggcatgct caagttgcca tttcttacac gacaagtccg
    26401 gtacatacca ttgactactt tgttgaattg gcgaaagctt accaagctat cggagcggac
    26461 tccatttgta tcaaagacat ggctggtgtc ttaactcctg aaataggtta ccaattggtc
    26521 aaatgtatca aggagaatac aactatccct cttgaggttc atacccatgc taccagtggt
    26581 atttcagaaa tgacttactt aaaagtagca gaagcaggag ctgatattat tgatacggcg
    26641 atttcctctt tttcaggggg aaccagtcag cctgccacag agtcaatggc gattgccttg
    26701 acggatttag gctttgacac aggcttggat atgcaagagg tggccaaagt tgcagaatat
    26761 ttcaacacta ttcgtgacca ctatcgagaa atagggattt taaatcctaa agtcaaagat
    26821 actgagccaa aaacattgat ttaccaagtt ccaggtggca tgctatcaaa cctattgagc
    26881 caattaaccg agcaaggcct aactgataaa tacgaagaag tcttagcaga agtacctaaa
    26941 gtaagggctg atcttgggta tccgccactc gtaacgccat tatcacaaat ggttggcaca
    27001 caggccttga tgaatatcat ctcaggggaa cgttacaagg tagttccaaa tgaaatcaaa
    27061 gactatgttc gagggctata cggtcaatca ccagcgccat tagcagaagg tatcaaagag
    27121 aaaatcattg gtgacgaagc agttattact tgcagaccag ccgacctaat cgagcctcaa
    27181 atgatttatc tacgtgacga gattgctcca tacgctcatt cagaagaaga cgtgttaagc
    27241 tatgcaagct tcccgcaaca agctagagat ttcttgggac gccgtgaaga tcctttctat
    27301 gatgttccgg ttcaagaagt tactgtacaa ctggacattc aagactaaca gagaatgact
    27361 aatcctctta accattaatc tccttggtga aacgcatttg ctgtaagaac aataccaaaa
    27421 acctacctga ttttgataat cagataggtt ttttatctta gtaatgtttg acagatgaag
    27481 tggcttgtat ctttttaata atgggttggg cttctagcga acaaaaataa tctaaagacg
    27541 ttttgggaag gagttttgcc agaaggtccc aatcattatc tttcaatgcc tgcctagccg
    27601 ttgaggcact gataggatct tgctgctgat tgatagcttt ccttggcaaa atatctagga
    27661 ggataccagc ttgctgcaac tgctctgcca taatttcatt gtaaatagca gttactcggc
    27721 tggttggctc ttctccaacg tatctttttg taatctgtaa ggtttgagca atctttagaa
    27781 aaatagcagt atccagcaaa gcttggctct taatcacgtc attgtcttct ttttggaaat
    27841 aggctgggaa agtcgcttgg ctaatcaaat aaggacctgt ttcatgatat atgacattat
    27901 caagatgagc tagaccttct tgaatcaacc tctttctcac cgaaaaagga attagactgc
    27961 ggtcttcact gaccataaac agatgaaccc aatcattttc tcttgcagct ttctctacta
    28021 aaaattgatg ccctaaggta aaggggttag cgttgataac aatagcagcg actttaccag
    28081 gtgtttgctc aggcttatta agtgtcatca gataatcttg aaaaccagtt tttttatttt
    28141 ccaagaaact gatgtgattg tcgatatgga caatttcagt aaaaccaaga tctttgaaaa
    28201 aagcagcaga agaggtcttc gtataaacaa aaagatgata gttgccaagg gcatattctt
    28261 catcaatgag atgactcaca atcctattaa gaagtccttc accttgatag gcactggata
    28321 cgcaaagaca gcgcaagcta tttccaaagt aagaaccggt agcaatcaca tcattttgcg
    28381 cattcatgat ggcacaagta taatctaaat gagcatccaa acgaatacct tcttgatgaa
    28441 gcaggttctt tacactagcc attgtcgttt tatctgaagg aaagactttt gaaatagtgt
    28501 agtaaggcat ttcttctcct tatctttaat tttattgaat cataacataa cttagtacca
    28561 aaacaggctg atctatctaa aggcaactct gttttttatc aacgccattt tttaaaagga
    28621 aatcgaatgt gtttgacaga ctcaaaagta tcagcgattt tttgattgcg ttccacagtc
    28681 cgcaagtttt cttggtctaa taagtcttga cttttttcca aaacatcaac agtaatgtag
    28741 tagagtttga tcaggcttcc tatcttatat ttaccagatt ttggagacat agcaaccact
    28801 tcattgagat tactatgaag ccatttttta tgaggcttag caggtatagt ggctactaca
    28861 aagccaagtt gttccaaatg agcttgtgcc tgatcaacag gaagattaat cacatcattt
    28921 atcgtgcgta attgcatctc tttttcatgt ttttgttcca tacgtttttc gataattggt
    28981 cgactgttat caatcgtttt tccaataatt tcagtagtgt caggaacaat tgacatcagt
    29041 ttcccaacta ctcctagagc ttttcttatt ctctttggca tcttttatct cctcaagtcg
    29101 tttcttttta ttataacaaa atgtctaaaa atagtatgcg ataaaccttg ttctaaaagg
    29161 ttaaatagat caattcaagg aaaaaatagg aggggatccg acaaaaaaac acaaagattc
    29221 taaaaaagtc aagctatttt agtttagcct ttgtcaatcg tatcagttca aatagaaaat
    29281 acaagttttg acaagaattt atttatgtaa caaactgtgt ttttgattct ttagtctatc
    29341 taacgataat gttatctgat tcttgttttc aaccaattga tgttaaagaa gctgtcttca
    29401 tgattctcta atattccttc aattt
//
LOCUS       DAXWIQ010000019        29346 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000019 DAXWIQ010000000
VERSION     DAXWIQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29346
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.019"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(243..857)
                     /locus_tag="ACGEUI_000868"
     CDS             complement(243..857)
                     /locus_tag="ACGEUI_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165713.1"
                     /translation="MKKTLTLLLALFAIGVTSSVRAEDEQNKFILDGLQEKVKEVSVS
                     DFSVGESKIKVWLPQAWSVKISREHSPKSSISNSGEQKPLSNSSENKEGQFSKRLPYG
                     TQHTIKLSSQLTKGERVTLTFRDEDFWGAGYCFYRDSLSIKEDKQYEEEIKKIEDDLE
                     RQDLENDALEMFKKQTEREANKPWHQRLSENIQDQWWNFKGLFQ"
     gene            1218..1454
                     /locus_tag="ACGEUI_000869"
     CDS             1218..1454
                     /locus_tag="ACGEUI_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFF8165714.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVDGKVLQAT
                     PYFLIHVTGAGEGFSMWAPTPCDVLAEDWIEVND"
     gene            1447..2145
                     /locus_tag="ACGEUI_000870"
     CDS             1447..2145
                     /locus_tag="ACGEUI_000870"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFF8165715.1"
                     /translation="MTKVVLVTGCASGIGYAQARYFLKQGHHVYGVDKSDKPDLSGNF
                     HFIKLDLSSELAPLFKVVPSVDILCNTAGILDAYKPLLDVSDEEVEHLFDINFFATVK
                     LTRHYLRRMVEKQSGVIINMCSIASFIAGGGGVAYTSSKHALAGFTRQLALDYAKDQI
                     HIFGIAPGAVKTAMTANDFEPGGLADWVARETPIGRWTKPDEVAELTGFLASGKARSM
                     QGEIVKIDGGWTLK"
     gene            2221..3180
                     /locus_tag="ACGEUI_000871"
     CDS             2221..3180
                     /locus_tag="ACGEUI_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8165716.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIDGIDLVAILSTVRSLTTAKDLA
                     KAHHMPLATSKYEAILGNEEIDTVYIGLPNHLHFAYAKEALLAGKHVICEKPFTMTAG
                     ELDELVVIARKRKLILLEAITNQYLSNMTFIKEHLDQLGDIKIVECNYSQYSSRYDAF
                     KRGDIAPAFNPKMGGGALRDLNIYNIHFVVGLFGRPKTVQYLANVEKGIDTSGMLVMD
                     YEQFKVVCIGAKDCTAEIKSTIQGNKGSLAVLGATNTLPQVQLSLHGHEPQVINHNKH
                     DHRMYEEFVAFRDMIDQRDFEKVNQALEHSRAVMAVLERAVHS"
     gene            3513..4850
                     /locus_tag="ACGEUI_000872"
     CDS             3513..4850
                     /locus_tag="ACGEUI_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8165717.1"
                     /translation="MEEDYNKREPEKFTQFLRRQKVVFFVAFFGYVCAYLVRNNFKLM
                     SNTIMVQNGWDKAQIAILLSCLTVSYGLAKFYMGALGDRVSLRKLFSISLGASALICI
                     LIGFFNSSMVVLGILLVLCGVVQGALAPASQAMIANYFPNKTRGGAIAGWNISQNMGS
                     ALLPLTIALLTSMGLVVPANGNILLAFLIPGVLVFLFALCCWKLGGDNPESEGLDSLR
                     TMYGDAGESAVASEEEKHNLSYWQLIWKYVFCNPSLLLVAAVNVALYFVRFGIEDWMP
                     IYLSQVANMSEAHIHFAISMLEWVAIPGSLVFAWLAVRYPNKMAKVGAIGLFVLAAIV
                     FVYERLTATGAPNYFLLLVIAGILGSLIYGPQLIVNILTINFVPLNVAGTAIGFVGVT
                     AYLIGNMGANWLMPILADGFGWFWSYIVVAALSAFSAVGYLILAKREEEIIKD"
     gene            5023..6405
                     /gene="glmU"
                     /locus_tag="ACGEUI_000873"
     CDS             5023..6405
                     /gene="glmU"
                     /locus_tag="ACGEUI_000873"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFF8165718.1"
                     /translation="MTNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAIS
                     PEKSVTVIGHKSEMVRAVLADQSAFVHQTEQLGTGHAVMMAETQLEGLEGHTLVIAGD
                     TPLITGESLKSLIDFHVNHKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANE
                     YEQQLKEINTGTYVFDNKRLFEALKCITTNNAQGEYYLTDVVAIFRANKEKVGAYILR
                     DFNESLGVNDRVALAIAETVMRQRITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEG
                     NVTLKGRTHIGSGTVLTNGTYIVDSEIGDNCVVTNSMIESSVLAAGVTVGPYAHLRPG
                     TTLDREVHIGNFVEVKGSHIGEKTKAGHLTYIGNAQVGSSVNVGAGTITVNYDGQNKY
                     ETVIGDHAFIGSNSTLIAPLEVGDHALTAAGSTISKTVPIDSIAIGRSRQVTKEGYAK
                     RLAHHPSRSK"
     gene            6436..6990
                     /locus_tag="ACGEUI_000874"
     CDS             6436..6990
                     /locus_tag="ACGEUI_000874"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFF8165719.1"
                     /translation="MKFEEKTLKRQTVFDGHIFKVVVDDVELPNNLGQSKRELIFHRG
                     AVAVLAITPERKIVLVKQYRKAIERVSYEIPAGKLEIGEEGSKLKAAARELEEETAYT
                     GTLTFLYEFYTAIGFCNEKITLFLATDLIQVANPKPQDDDEVIEVLELTYQECMDLVA
                     QGKLADAKTLIALQYYALHFGGEQ"
     gene            6990..7241
                     /gene="macP"
                     /locus_tag="ACGEUI_000875"
     CDS             6990..7241
                     /gene="macP"
                     /locus_tag="ACGEUI_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFF8165720.1"
                     /translation="MGRPLLTDDIIEKARRMETFEPDDAVNFDTKVMTLPEKDDKARI
                     YKSRRIENAKRSQLQSKLNVILIAVMLLIAILVYAIFYL"
     gene            7261..7956
                     /locus_tag="ACGEUI_000876"
     CDS             7261..7956
                     /locus_tag="ACGEUI_000876"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFF8165721.1"
                     /translation="MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELI
                     LVQSGVGKVMSAMTVAILVEHFKAQAIINTGSAGAVASHLAIGDVVVADRLVYHDVDA
                     TAFGYAYGQMAGQPLYYDCDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQDKIDQ
                     IKTAFSDVLAVEMEGAAIAQAAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRS
                     AQTLMTFLENLPV"
     gene            8107..8448
                     /locus_tag="ACGEUI_000877"
     CDS             8107..8448
                     /locus_tag="ACGEUI_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165722.1"
                     /translation="MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNEGYHQHLTD
                     EKSHLQYSKDNAQLQLRNILDGYQNDLGRHYSSYYYYNLRTVMGLSSEQDIEKHYEEL
                     KNKLHDMYNHY"
     gene            complement(8549..9196)
                     /locus_tag="ACGEUI_000878"
     CDS             complement(8549..9196)
                     /locus_tag="ACGEUI_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFF8165723.1"
                     /translation="MTPNKEDYLKCIYEIGEQEPKITNKMVAEKMHVSAPAVSEMIKK
                     MISQGWIVKDKAKGYLLKDKGYALVANLYRKHRLIEVFLIHQLGYNTQEVHQEAEVLE
                     HTVSDSFIDRLDKILDFPDFCPHGGTIPRYGQPLVEMNTTTLNTITELGRFRLSRIHD
                     HFDLIQYLEAHHLNINTELTLTQIDTFAKTYTICYGDKELVIPENIAKQLYVTAL"
     gene            9342..10274
                     /locus_tag="ACGEUI_000879"
     CDS             9342..10274
                     /locus_tag="ACGEUI_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFF8165724.1"
                     /translation="MGKRMSLILGAFLSVFLLVACSSTGTKTAKSDKLKVVATNSIIA
                     DMTKAIAGDKIDLHSIVPIGQDPHEYEPLPEDVEKTSNADVIFYNGINLEDGGQAWFT
                     KLVKNAQKTKNKDYFAVSDGIDVIYLEGASEKGKEDPHAWLNLENGIIYSKNIAKQLI
                     AKDPKNKETYEKNLKAYVAKLEKLDKEAKSKFDAIAENKKLIVTSEGCFKYFSKAYGV
                     PSAYIWEINTEEEGTPDQISSLIEKLKVIKPSALFVESSVDRRPMETVSKDSGIPIYS
                     EIFTDSIAKKGKPGDSYYAMMKWNLDKISEGLAK"
     gene            10338..11063
                     /locus_tag="ACGEUI_000880"
     CDS             10338..11063
                     /locus_tag="ACGEUI_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8165725.1"
                     /translation="MITTNNLCVTYDGNSNALEAINVTIEGPSIVGIIGPNGAGKSTF
                     MKAILNLIDYQGHVTVDGKDGRKLGHTVAYVEQRSMIDYNFPITVKECVALGTYSKLG
                     LFRRVGKKQFEQVDKVLKQVGLEDFGHRPIKSLSGGQFQRMLVARCLIQESDYIFLDE
                     PFVGIDSVSEKIIVDLLKELKMAGKTILIVHHDLSKVEHYFDKLMILNKHLVAYGNVC
                     EVFTVDTLSKAYGNHLILGKEMV"
     gene            11064..11918
                     /locus_tag="ACGEUI_000881"
     CDS             11064..11918
                     /locus_tag="ACGEUI_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFF8165726.1"
                     /translation="MSMKFFEGLMSYHFLQNALITAVVIGIVSGAVGCFIILRSMSLM
                     GDAISHAVLPGVALSFILGVNFFIGAIIFGLLASVIITYIKENSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDSDKWITIGVSIFVLVVISLFFKELLLT
                     SFDPILAKSMGVKVNAYHYLLMVLLTLVAVTAMQSVGTILIVALLITPAATAYLYANS
                     LKVMLVMSSLLGALASVLGLYLGYTFNVAAGSSIVLTSAMMFLISFFVSPKQGYLKRW
                     MQKKEKTP"
     gene            complement(12066..12872)
                     /locus_tag="ACGEUI_000882"
     CDS             complement(12066..12872)
                     /locus_tag="ACGEUI_000882"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8165727.1"
                     /translation="MKKLLSLSLVAISLLSLSACESVDRAIKGDKYIDEKTAKEESEA
                     ASKAYEESIQKALKADASQFPQLTKEVGKEEAKVVMRTSQGDITLKLFPKYAPLAVEN
                     FLTHAKKGYYDNLTFHRVINDFMIQSGDPKGDGTGGESIWKGKDPKKDAGNGFVNEIS
                     PFLYHIRGALAMANAGANTNGSQFYINQNKKNQSKGLSSTNYPKPIISAYEHGGNPSL
                     DGGYTVFGQVIDGMDVVDKIAATSINQNDKPEQDITITSIDIVKDYRFKN"
     gene            13089..15494
                     /locus_tag="ACGEUI_000883"
     CDS             13089..15494
                     /locus_tag="ACGEUI_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFF8165728.1"
                     /translation="MVKRNQRKKSAPKKRLTKAEVEKQRAIKRMILSVLMALLLIFAM
                     LRLGVFGVTTYNMIRFLVGSLAYPFMFAWLIYLFCFKWLRQKDGMIAGVVIAFLGLLV
                     EWHAFLFAMPRMLDQDIFLGTARLITRDLLALRVTEFVGGGMLGALLYKPIAFLFSNI
                     GSYFIGFLFILLGLFLMTPWDIYDVSHFVKEAVDKLAVAYQENKEKRFIKREEHRLQA
                     EKEALEKQAQEEEKRLAELTVDPETGEIVEDSQSQVSYDLAEDMTKEPEILAYDSHLK
                     DDETSLFDQEDLAYAHEEIGAYDSLSALASSEDEMDMDEPVEVDFTPKTHLLYKLPTI
                     DLFAPDKPKNQSKEKNLVRKNIKVLEDTFQSFGIDVKVERAEIGPSVTKYEIKPAVGV
                     RVNRISNLADDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSFRELWEQSDANP
                     ENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISSILMKARPDQVK
                     FMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFSKIGVRNIA
                     GYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIH
                     MILATQRPSVDVISGLIKANVPSRMAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPI
                     DENHPVRLQGSFISDDDVERIVNFIKDQAEADYDDAFDPGEVSDNDPGFSGNGGAAEG
                     DPLFEEAKALVLETQKASASMIQRRLSVGFNRATRLMDELEEAGVIGPAEGTKPRKVL
                     QTN"
     gene            complement(15564..15917)
                     /locus_tag="ACGEUI_000884"
     CDS             complement(15564..15917)
                     /locus_tag="ACGEUI_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFF8165729.1"
                     /translation="MMIYKLTALAFLIVTPLFAMIVTSLFKLNKKGFKFPDVALLLFA
                     IEIVLVSGKFFTHNLLPYYLIIMSLLAIVISLLLVIRTQSFSYHRFMKLFWRVGFLVT
                     VIFYLILVIFIFTLA"
     gene            16161..16586
                     /gene="rplK"
                     /locus_tag="ACGEUI_000885"
     CDS             16161..16586
                     /gene="rplK"
                     /locus_tag="ACGEUI_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFF8165730.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGTPNTTKVATVTR
                     AQVQEIAETKMPDLNAANIEAAMRMIEGTARSMGFTVTD"
     gene            16692..17381
                     /gene="rplA"
                     /locus_tag="ACGEUI_000886"
     CDS             16692..17381
                     /gene="rplA"
                     /locus_tag="ACGEUI_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFF8165731.1"
                     /translation="MAKKSKQMRAALEKVDSTKAYSVEEAVALVKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTQRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAIVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMANVSITSTQ
                     GVGIKVDPNSL"
     gene            17704..18432
                     /gene="pyrH"
                     /locus_tag="ACGEUI_000887"
     CDS             17704..18432
                     /gene="pyrH"
                     /locus_tag="ACGEUI_000887"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFF8165732.1"
                     /translation="MEPKYQRILIKLSGEALAGEKGVGIDIPTVQAIAKEIAEVHVSG
                     VQIALVIGGGNLWRGEPAADAGMDRVQADYTGMLGTVMNALVMADSLQHYGVDTRVQT
                     AIPMQNVAEPYIRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEADAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHGEVIKRGLKIMDATASTLSMDNDIDLVVFNMN
                     EAGNIQRVVFGEHIGTTVSNKVCD"
     gene            18461..19018
                     /gene="frr"
                     /locus_tag="ACGEUI_000888"
     CDS             18461..19018
                     /gene="frr"
                     /locus_tag="ACGEUI_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFF8165733.1"
                     /translation="MANAIIETAKERFAQSHQSLSREYASIRAGRANASLLDRIQVDY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRKELAKEVKKVGENAKIAIRNIRRDAMDDAKKQEKAKEITEDELKTLEKDI
                     QKATDDAIKEIDRMTAEKEKELLSV"
     gene            19127..19984
                     /locus_tag="ACGEUI_000889"
     CDS             19127..19984
                     /locus_tag="ACGEUI_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HFF8165734.1"
                     /translation="MNDLLATVITGLIKEENANDYFIHKEGFTFTLSKAEGERQIGDM
                     VTGFAYTDIEQKARLTTKEIRSTRTSYGWGEVTEVRRDLGVFVDTGIPNKEIVVSLDV
                     LPEMKELWPKKGDKLYIRLDVDKKDRIWGLPAEPEVFQKMASPAYNNMQNQHWPAIVY
                     RLKLTGTFVYLPENNMLGFIHSSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMIVTYLEANGGFMTLNDKSSPEEIKASFGISKGQFKKALGGLMKAKRIKQ
                     DATGTELIG"
     gene            20057..20566
                     /gene="msrA"
                     /locus_tag="ACGEUI_000890"
     CDS             20057..20566
                     /gene="msrA"
                     /locus_tag="ACGEUI_000890"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8165735.1"
                     /translation="MERAIFAGGCFWCMVQPFEEQAGILSVRSGYTGGHLPNPSYEQV
                     CAKTTGHTEAVEIIFDPKQIAYKDLVELYWTQTDPTDAFGQFEDRGDNYRPVIYYTTE
                     RQKEIAEQSKANLQASGRFDQPIVTTIEPAEPFYLAEDYHQGFYKKNPKRYAQSSAIR
                     HQFLEENWS"
     gene            20563..20778
                     /locus_tag="ACGEUI_000891"
     CDS             20563..20778
                     /locus_tag="ACGEUI_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFF8165736.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADLVFDDTTFPKHTNDFELI
                     SRYLEDQASFSFNLGQFDEIWEDYLAH"
     gene            20934..22103
                     /locus_tag="ACGEUI_000892"
     CDS             20934..22103
                     /locus_tag="ACGEUI_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8165737.1"
                     /translation="MIITKKSLFVTSVALSLVPLATAQAQEWTPRSVTEIKSELVLVD
                     NVFTYTVKYGDTLSTIAEAMGIDVHVLGDINHIANIDLIFPDTILTANYNQHGQATNL
                     TVQAPASSPASVSHVPSSEPLPQASATSQPTVPMAPPATPSDVPTTPFASAKPDSSVT
                     ASSELTSSTNDVSTELSSESQKQPEVPQEAVPTPKAAETTEVEPKTDISEAPTSANRP
                     VPNESASEEVSSAAPAQAPAEKEETSAPAAQKAVADTTSVATSNGLSYAPNHAYNPMN
                     AGLQPQTAAFKEEVASAFGITSFSGYRPGDPGDHGKGLAIDFMVPENSALGDQVAQYA
                     IDHMAERGISYVIWKQRFYAPFASIYGPAYTWNPMPDRGSITENHYDHVHVSFNA"
     gene            22417..24189
                     /locus_tag="ACGEUI_000893"
     CDS             22417..24189
                     /locus_tag="ACGEUI_000893"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFF8165738.1"
                     /translation="MYYTSGNYEAFATPRKPEGVDQKSAYIVGTGLAGLAAAVFLIRD
                     GHMAGERIHLFEELPLAGGSLDGIEKPHLGFVTRGGREMENHFECMWDMYRSIPSLEI
                     PGASYLDEFYWLDKDDPNSSNCRLIHKRGNRVDDDGQYTLGKQSKELIHLIMKTEESL
                     GDQTIEEFFSEDFFKSNFWVYWATMFAFEKWHSAVEMRRYAMRFIHHIDGLPDFTSLK
                     FNKYNQYDSMVKPIIAYLESHDVDIQFDTKVTDIQVEQTAGKKVAKTIHMTVSGEAKA
                     IELTPDDLVFVTNGSITESSTYGSHHEVAKPTKALGGSWNLWENLAAQSDDFGHPKVF
                     YQDLPAESWFVSATATIKHPAIEPYIERLTHRDLHDGKVNTGGIITITDSNWMMSFAI
                     HRQPHFKEQKENETTVWIYGLYSNSEGNYVHKKIEECTGQEITEEWLYHLGVPVDKIK
                     DLASQDYINTVPVYMPYITSYFMPRVKGDRPKVIPDGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYSFLNVERGIPEVFNSAYDIRELLKAFYYLNDKKAIKDMDLPIPAL
                     IEKIGHKKIKDTFIEELLKDANLM"
     gene            24348..25400
                     /locus_tag="ACGEUI_000894"
     CDS             24348..25400
                     /locus_tag="ACGEUI_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFF8165739.1"
                     /translation="MQEYSIDITLTHPDDVLALFGSNERHLKLIEAHLGVIVHARTER
                     VQVIGDDEEAVELARLTIKALLVLVGRGMVVNTSDVVTALSMAESHQIDQFMALYEEE
                     IIKDNYGKAIRVKTLGQKTYVDSVKRHDVVFGVGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYHILGKEQTTRLMERDVIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDTSQIDLPRNVKS
                     GLIDATQKLQGIKQIDFVYFSAKDVVRHPVVADIIKAYETSSEEMKDLLKPKASDVEA
                     DYQPGLTKYPVIGQEH"
     gene            25446..26021
                     /locus_tag="ACGEUI_000895"
     CDS             25446..26021
                     /locus_tag="ACGEUI_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFF8165740.1"
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARIIIVGQAPGIV
                     AQGTKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFA
                     AKWHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPS
                     PRNQLWLAKNPWFEQDLLPILQKRVEAILTK"
     gene            26180..26677
                     /gene="ybeY"
                     /locus_tag="ACGEUI_000896"
     CDS             26180..26677
                     /gene="ybeY"
                     /locus_tag="ACGEUI_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFF8165741.1"
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGEL
                     FISIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAY
                     GLTRQ"
     gene            26658..27065
                     /locus_tag="ACGEUI_000897"
     CDS             26658..27065
                     /locus_tag="ACGEUI_000897"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8165742.1"
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLL
                     SACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNA
                     KDMAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            27185..28081
                     /gene="era"
                     /locus_tag="ACGEUI_000898"
     CDS             27185..28081
                     /gene="era"
                     /locus_tag="ACGEUI_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFF8165743.1"
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIM
                     GIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDD
                     MIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFHSQMDFKEVVPISALEGNNVPTL
                     IKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKR
                     DEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVK
                     VKKNWRDKKLDLADFGYNEKEY"
     gene            28101..28577
                     /locus_tag="ACGEUI_000899"
     CDS             28101..28577
                     /locus_tag="ACGEUI_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFF8165744.1"
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTNDDGKVLMQLRGDKKT
                     WTIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFI
                     YELTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
     gene            complement(28882..29136)
                     /locus_tag="ACGEUI_000900"
     CDS             complement(28882..29136)
                     /locus_tag="ACGEUI_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165745.1"
                     /translation="MSGLFYHKSYFFKSKQVYDLLNEIRMRYPHAHIILIGNHINYEE
                     IFKNHYRVFGVIDTTSHKSLRSIRDQIQLYLDELYNSNNG"
BASE COUNT         8912 a         5219 c         6394 g         8821 t
ORIGIN      
        1 tcctcgttca cctcgtaccc cacaaactac tttatcctaa aaaaccataa aatcaacatt
       61 gaggactttt ttggttccca taaattgtta actatgtcat tttagagcaa tcatatagag
      121 actaagatat aattttacca aagttttgaa agctattgaa gagtatcttg attttgtcta
      181 ggttctaagc acctgtaagg gtgctttttg tgctttagcc aacctcatag tctagtaaac
      241 cctcactgaa acagtccctt aaagttccac cactgatctt ggatgttttc gcttaaccgc
      301 tgatgccaag gtttattagc ctctctttcg gtttgttttt taaacatctc tagtgcatca
      361 ttttcaagat cttgtctctc taggtcatcc tcaattttct taatttcttc ttcgtattgt
      421 ttgtcttctt ttatggatag tgaatctcta tagaagcagt aacctgctcc ccaaaaatct
      481 tcatctctga atgtcaaagt gactctctca ccttttgtaa gttgggatga taatttaata
      541 gtatgttggg taccataagg taatctttta gaaaattgac cttctttatt ctctgagcta
      601 ttgcttaaag gtttttgttc tccagaatta gaaatgcttg attttggtga atgttctcta
      661 gaaattttga ccgaccaagc ttgaggaagc cagactttga ttttagattc tccaacagaa
      721 aaatctgata cactaacttc ttttactttt tcctgtaatc catcaagtat aaacttattc
      781 tgttcatcct ccgctctgac gctactagtt accccgatgg caaagagtgc cagtagcaag
      841 gttaatgttt ttttcatttt ttcctctcct tttgctcatt tttaagataa gacttattat
      901 accaattctt gcgctaaaaa gatagggagt ttatgtcttg tggttgggaa atggcgtgac
      961 tattcttggt aggggcagac aggttttttc ctagccagtt gctaaccgtt acccttttac
     1021 ctttgcaagc cgtttgggca tcaggtcgtg ttttgaggtt gccaatactt ttagttgctt
     1081 tttgacaaaa atgcgttatg atagactaaa taaaagaaga tagcattttc gatgaatgat
     1141 gcgtgagtcc acaaatttat ttttaggagt ttatagactg cctaaaagtg tctatttcgt
     1201 caataaagga gaacaatatg acatttgaag agattttacc aggtttaaaa gccaagaaaa
     1261 aatatgtacg tacaggttgg ggtggcgctg aaaattatgt gcagttgttt gatacccttg
     1321 aagtggatgg gaaagtacta caggcgacgc cttattttct gattcatgtt actggtgcag
     1381 gtgaaggttt ttccatgtgg gcaccgacac catgtgatgt gttagcagag gattggattg
     1441 aggtcaatga ctaaagttgt tttagtgacg ggctgtgcta gtgggattgg ttatgcacag
     1501 gcccgctatt ttttgaaaca agggcatcac gtatatggtg tggataagtc ggataaaccg
     1561 gacttaagcg gtaactttca ctttatcaaa ttggatttat cctctgagtt agccccacta
     1621 tttaaagtgg tccccagtgt tgatattttg tgtaatactg cagggatttt agatgcctac
     1681 aagccacttt tagacgtcag tgatgaagaa gtggaacacc tatttgatat taattttttt
     1741 gcgacggtga agttaacacg tcattatctt agacgtatgg tggaaaagca gtcaggtgtt
     1801 atcatcaata tgtgctcaat agcaagtttt atagcgggtg gcggtggtgt tgcctatacg
     1861 agttctaagc atgcccttgc gggattcacg cgccagctgg ctttggatta cgccaaagat
     1921 cagattcaca tttttggtat agcaccaggt gctgtcaaaa ccgctatgac ggctaacgat
     1981 tttgagcctg gtggcttagc agactgggta gctcgtgaga cgccaattgg gcgttggact
     2041 aaaccagatg aagttgctga attaacggga tttcttgctt caggaaaagc gagatcaatg
     2101 caaggcgaga ttgtcaaaat tgacggtggc tggactttga aatgatcaca tgccttagct
     2161 tgagctggcg cagtaagccc tagaggtgtg atgagtcaaa cagtcaaagg agatgaacaa
     2221 atgaaattag cggttttggg gacaggtatg attgttaagg aagttttgcc tgtcttgcag
     2281 aaaattgatg gtattgattt agttgctatc ttatcaacag ttagaagttt aacgacagct
     2341 aaagacctcg ctaaggcaca tcacatgcct ctagctacga gtaaatatga ggctatctta
     2401 ggcaatgagg agattgacac ggtttatatt ggactgccga atcacctgca ttttgcctat
     2461 gctaaggagg cccttctagc tggcaaacat gtcatttgtg aaaagccctt taccatgaca
     2521 gcaggagaat tagatgaact ggtggtaatc gctagaaaac gaaaactcat tttgttagaa
     2581 gcgattacga atcagtacct gagtaacatg acctttatca aagagcatct tgaccaactt
     2641 ggcgatatca aaattgtaga atgcaattat tcacaatatt cttcacgcta cgatgccttc
     2701 aaacgaggtg atatcgcgcc agcttttaat cctaaaatgg gtggtggagc tttacgtgat
     2761 ttaaacatct acaacattca ttttgtggta gggttatttg gtcgccctaa aacggttcaa
     2821 tatttagcca atgtcgaaaa aggcattgac acctcaggca tgcttgtcat ggactatgag
     2881 cagtttaaag tggtgtgtat cggggccaaa gactgcactg cagaaatcaa atcaaccatc
     2941 caaggtaaca aagggtcact ggcagtgctt ggagcaacaa ataccctccc tcaggttcag
     3001 ttaagtctac acggtcacga accacaagtg attaaccaca acaagcatga ccaccggatg
     3061 tatgaagagt ttgtcgcttt tagggacatg attgaccaaa gagactttga gaaagtgaat
     3121 caggctttgg aacatagccg agctgtcatg gctgtgttgg agcgcgctgt tcattcgtga
     3181 tggtaatctg gtagccattg ccttttgtgc aggtgctatg gtaatggttg aaagacgtgt
     3241 tttcacgagg gggagccccc tcgtgctttt ttggcaaaaa taacttgttc aggatttggg
     3301 ctttctttgt gagatgagag atgcctgacg aattgatttg attaaaataa tgataaagcc
     3361 aaacgattta agaaatgacc gttcggaaat tctgccccaa aggggctcta gaagcagtaa
     3421 aatcgaattt gtaagcgttg acactgttgc ataactctgt tacactaaga gaatgtcatg
     3481 acaaaaatta cagtgtagga gtttaattgt ttatggaaga ggattataac aaaagagaac
     3541 ctgagaagtt tacgcaattt ctgcgccgtc aaaaggtcgt cttttttgtc gcattttttg
     3601 gttatgtgtg tgcttacttg gtacgcaaca atttcaaact aatgtcaaac actattatgg
     3661 tgcaaaatgg ttgggacaag gctcaaattg cgatcttgtt atcgtgtttg accgtttctt
     3721 atggcttagc aaaattctat atgggagcct taggggatcg tgttagcttg aggaagctct
     3781 tttcaatcag cttgggtgca agcgctttga tttgtatttt aattggtttt ttcaatagtt
     3841 caatggtggt attaggaata ctgctagtct tgtgtggtgt cgtacaaggt gccttggcac
     3901 ctgcctcaca agccatgatt gccaattatt ttccaaataa aacacgcggc ggagccatcg
     3961 ctggatggaa catttctcaa aacatgggat cagccctctt gccattaacc atcgccttgc
     4021 tcaccagtat gggcttggtg gtaccagcta atggcaatat tttacttgcc tttttaatcc
     4081 ctggcgtttt agtgttcttg tttgctttgt gttgctggaa gcttggcggt gataaccctg
     4141 agtctgaggg acttgactct cttcgaacga tgtatggtga tgcgggcgag tcagctgttg
     4201 ccagtgaaga agaaaagcat aacctgtctt attggcaact catctggaaa tacgtttttt
     4261 gtaacccgtc gctcttactt gttgctgctg tcaatgtggc cctttatttt gttcgttttg
     4321 ggattgaaga ctggatgccg atctacttgt cacaagtagc caatatgtca gaggctcata
     4381 tccattttgc gatttcaatg ttagagtggg tcgctattcc aggctcgctg gtatttgcgt
     4441 ggttagcggt tcgttatcct aataaaatgg ccaaggttgg ggctattggg ctttttgtgt
     4501 tagcggctat tgtctttgtc tatgaacgct tgactgccac aggtgctcca aattatttct
     4561 tgttgcttgt tattgcaggt attttagggt cattgattta tggcccacag ttgatcgtga
     4621 atattttaac aatcaacttt gttcctttaa atgttgcagg aacagcgatt ggttttgtag
     4681 gagtaacagc ttatctcatc ggcaatatgg gagcaaactg gctgatgccg attttggcag
     4741 atggttttgg ctggttttgg tcatatattg tcgttgcagc cttatctgct ttttcagcgg
     4801 ttggttattt gattttagcc aaacgtgagg aagaaatcat caaagattag ctaggcaaat
     4861 gcctaagaaa gacagactta ttgctgaagt cctgtctttt ttagttatca caagccgcaa
     4921 atgacactat aaaagacaag aaaatgctag gatgtttcag gaaaaaaaga gaaaatatgc
     4981 tataatgaga aggtatttaa attatatagt aaggaaaaac aaatgacaaa ttatgccatt
     5041 attttagcag cgggcaaagg gactcgcatg acttctgatc tcccaaaagt tcttcataaa
     5101 gtatctggac taaccatgct tgagcatgtt tttcgtagtg taaaagcgat tagtcctgaa
     5161 aaatccgtga cggttattgg tcacaaatca gaaatggtac gtgctgtctt agcagatcaa
     5221 tcagcctttg tccatcaaac agagcagtta ggaactggcc atgccgtgat gatggcagaa
     5281 acacaactag aaggactaga agggcatacc ttggtgattg caggagatac tcccttgatc
     5341 actggagaaa gcctgaagag tctgattgat tttcatgtga atcataaaaa tgtcgccact
     5401 attttaacag ctacggctca agatccattt ggttatggtc gtattgttcg caacaaagat
     5461 ggcgaagtga tcaagattgt tgagcaaaaa gacgccaatg agtatgagca acaattaaaa
     5521 gaaatcaata caggaaccta tgtgtttgat aacaagcgtc tctttgaagc gctcaaatgc
     5581 atcactacca acaatgctca aggagagtat tacctgactg acgttgtggc tatttttaga
     5641 gcaaacaaag aaaaggtagg agcctatatc ctgagagact tcaatgaaag tctgggagta
     5701 aatgatcgtg tggccttagc aatagctgaa acagtgatgc gtcagcgcat tacccaaaaa
     5761 catatggtca atggggtgac tttccaaaat cctgaaactg tttatattga aagcgatgtt
     5821 gagattgctc cagacgtcct tatcgaagga aatgtgacct taaaagggcg tacacatatc
     5881 ggctcaggaa ctgtcttgac gaatggtact tatattgtgg attctgaaat tggtgataac
     5941 tgtgttgtta ccaattcgat gatcgaatca tctgttttag ctgcaggagt tacggttggc
     6001 ccttacgccc accttcgacc gggaacgacc ttagataggg aagtccatat cggtaacttt
     6061 gtcgaggtca agggttctca cattggtgaa aaaacaaagg ctgggcatct gacttatatt
     6121 ggaaacgctc aggttgggtc ttcagtcaat gttggggctg gaaccatcac tgtcaactac
     6181 gacggccaaa acaaatatga aacagtcatt ggagatcacg cttttattgg gagcaactcg
     6241 actctcattg cacctttgga agttggggat cacgctttaa cagcagcagg ttcaacgatt
     6301 tcaaaaacag ttcctatcga tagcattgct attggtcgta gccgccaagt cacaaaagaa
     6361 ggttatgcca agcgcctagc gcatcaccca agtcgaagca aataattcaa gatctattta
     6421 gtaaggaggc caggcatgaa atttgaagaa aaaacgctta agagacaaac agtttttgat
     6481 ggtcatattt ttaaagttgt tgtggatgat gttgaacttc ccaacaatct ggggcaatcc
     6541 aagcgagaat taatttttca tcgtggtgcc gttgctgttt tggctattac tcctgaacgt
     6601 aagattgtcc ttgtcaaaca ataccgtaag gctattgaac gtgtctcata cgagatccca
     6661 gcgggtaagc tagagattgg tgaggaaggc tcaaagctaa aagctgctgc acgtgagcta
     6721 gaagaagaaa cagcctatac ggggacattg acgtttttgt atgaatttta tacagcgatt
     6781 ggtttttgca acgaaaaaat cacacttttc ttagcgacag acctaataca agttgccaat
     6841 cccaaacctc aagacgacga tgaagttatc gaggtcttag agttgacata tcaagagtgc
     6901 atggacttag tcgctcaagg taaacttgct gacgctaaaa cgttgattgc tttgcaatac
     6961 tatgccttac attttggagg tgagcagtga tgggaaggcc attgttgacc gatgatatca
     7021 tcgaaaaagc gagacgaatg gaaacatttg agccagatga tgctgtaaac tttgacacaa
     7081 aagtaatgac gctaccagaa aaagatgata aagcacgcat ttataaaagt cgtcggattg
     7141 aaaatgccaa acgcagccaa ttgcaatcta aattaaatgt gattttgata gctgttatgc
     7201 tgttaattgc catcttggta tatgctatct tttatcttta aaaaaacgta aggaaatgat
     7261 atgaaaatag gaattattgc tgctatggag gaggagttgt ccctgctctt agcaaatctt
     7321 ttggatgctc aagaacatca ggtgctttca aaaacttact atacgggtcg ttttggcaag
     7381 catgagctca ttttggtaca gtcgggtgtt ggaaaagtga tgtctgctat gacagttgcg
     7441 atcttggttg agcatttcaa agcccaagct attatcaata caggatcagc aggggcagtc
     7501 gcttcacatt tagcgatcgg agatgtggtg gttgcagatc ggttagtgta tcatgatgtg
     7561 gatgctactg catttggtta tgcttatggt caaatggctg gtcaaccatt atactatgat
     7621 tgtgatccac agtttgtggc tatcttcaaa caagtcctaa aacacgaaaa gacaaatggc
     7681 caagttggtt tgattgcaac aggagacagt tttgttgctg gtcaggacaa gattgaccag
     7741 atcaaaacag cttttagcga cgtgttagca gtagaaatgg aaggcgcagc tattgctcaa
     7801 gcagcacata ctgcagggaa acccttcatc gttgttcgtg cgatgagtga taccgcagcg
     7861 catgatgcca atattacttt tgatcaattt attatagaag ctggcaagcg ttcggcccag
     7921 acattaatga catttttaga aaacttacct gtttaactca ctttatcgtg agacaattag
     7981 ctggataatc tctattttag gagtctggtc acttcttgtt ttgaaaccta aaaataagaa
     8041 ttgcaatcac ttgaccaaac gctataatgg gttaagtaac gatagattac taaaaggagt
     8101 ctaaaaatga cactaaaaaa acactattat cttctcagcc tgctagctct tgtaacggtt
     8161 ggtgctgcct ttaacacaag ccagagtgtc agtgcacaag tttatagcaa tgaagggtat
     8221 caccagcatt tgactgatga aaaatcacac ctgcaatata gtaaagacaa cgcacaactt
     8281 caattgagaa atatccttga cggctaccaa aatgacctag ggagacacta ctctagctat
     8341 tattactaca acctaagaac cgttatggga ctatcaagtg agcaagacat tgaaaaacac
     8401 tatgaagagc ttaagaacaa gttacatgat atgtacaatc attattaagt caaaggctta
     8461 aaaatggcga ctagcccctc aaaaaaagag gccacactca tttaaattta aatgagtgtg
     8521 gcctcttttt ttgaggagac gcaaggggtt aaagggctgt gacataaagt tgcttagcaa
     8581 tattttcagg gatgacaagt tccttgtcgc cataacaaat cgtataggtt tttgcaaagg
     8641 tatcaatttg agttaaggtt agctcggtat tgatattgag gtgatgtgct tctagatact
     8701 ggattaaatc aaagtggtcg tggattctgc ttagacgaaa ccgtcccaac tccgtgatgg
     8761 tgttgagagt tgtagtgttc atttcgacaa gaggctgacc atagcgagga atagtccctc
     8821 cgtgtgggca gaaatctgga aaatcaagga tcttgtctaa acggtcgata aaggagtctg
     8881 agacagtgtg ctctaagact tcagcttctt ggtgcacttc ttgggtatta tagcccagct
     8941 gatgaatcaa aaatacctca atcaagcggt gtttgcggta gaggttagca acaagagcat
     9001 agcctttgtc ttttagcaaa tagccttttg ctttatcttt gacaatccag ccttgtgaaa
     9061 tcattttttt aatcatctct gaaacggcag gtgccgaaac gtgcatcttt tctgcaacca
     9121 ttttatttgt aatcttgggt tcttgttctc caatttcata aatacatttc aagtaatctt
     9181 ctttattagg cgtcatatcg tttggccttt ctcttattta taatgattat tatatcagaa
     9241 tttttaggaa aaagcttgac aaaaagccta aaagaggata tccttatagc ataattaact
     9301 aaacttaatt aaataaataa tatatcctaa ggagagaaat gatgggaaag agaatgagcc
     9361 tcattttggg tgctttttta tctgtttttc ttttagtagc ttgttcgtca actggcacta
     9421 aaactgctaa gagtgataaa ttaaaagtcg tggcaaccaa ttcaattatt gccgacatga
     9481 caaaagctat tgctggtgat aaaatcgatc tgcacagcat tgtgccaatc ggtcaagacc
     9541 ctcatgagta cgaaccatta ccagaagatg ttgaaaaaac aagtaatgct gatgtgattt
     9601 tctataatgg tatcaatcta gaagatggcg ggcaagcttg gttcaccaaa ctagtgaaaa
     9661 atgctcaaaa aacgaaaaac aaagattact ttgccgtgtc tgatggcatt gatgtgattt
     9721 acttggaagg tgcaagcgaa aaaggaaaag aagatccaca tgcttggtta aatctcgaaa
     9781 acggaatcat ttattcaaaa aacattgcca aacaattgat tgcaaaggat cctaagaaca
     9841 aagaaactta tgaaaagaac ctaaaagctt atgtggctaa attggaaaaa ctagacaaag
     9901 aagccaaatc aaaatttgat gctattgcag aaaataaaaa attgattgtg actagtgaag
     9961 gctgcttcaa gtacttttca aaagcttacg gtgtcccatc tgcttatatc tgggaaatta
    10021 acaccgaaga agaaggaaca ccagatcaaa tttcatcatt gattgaaaaa ctaaaagtca
    10081 tcaagccatc tgcgcttttt gtagagtcaa gtgtcgatag acgccctatg gaaactgttt
    10141 ctaaagatag tggtattcct atttattctg agatctttac agattcaatt gctaaaaaag
    10201 gtaaacctgg cgatagttat tatgctatga tgaaatggaa ccttgacaaa atttctgaag
    10261 gtctagcaaa ataagataac aataaagcac agcgatttta gtgttgtgct ttgttttatt
    10321 tatgtaaagg agacaaaatg attacaacta acaatctttg tgtgacctac gatggcaaca
    10381 gtaatgcttt agaagcgatt aatgtgacaa ttgaaggtcc ttctatcgtt ggtattattg
    10441 gtcctaatgg agctggaaaa tcaaccttta tgaaagctat cttaaatctt attgattatc
    10501 aaggtcacgt aacggttgat ggcaaagatg gtcgtaagct tgggcataca gtagcttacg
    10561 tagaacaacg aagcatgatt gactataatt ttcctattac tgttaaagaa tgtgttgcgc
    10621 tagggactta tagtaaatta ggcctttttc gccgagtagg aaaaaaacaa tttgagcagg
    10681 ttgataaggt tctcaaacaa gttggtttag aagactttgg ccatcgaccg ataaaatcct
    10741 tatctggtgg tcagtttcag cggatgttgg ttgcgcgttg cttgattcaa gaatcagact
    10801 atattttctt agatgaaccg tttgttggca ttgactcggt cagcgaaaaa atcattgtag
    10861 atcttttgaa agagctcaaa atggctggca aaacgatttt aattgtacat catgatttga
    10921 gcaaagtaga gcattatttt gacaaactca tgattttaaa taaacatttg gtggcttatg
    10981 gcaacgtttg tgaggtattt actgttgata ccttgtcaaa agcttatggc aatcatttga
    11041 ttttaggaaa ggagatggtg taaatgtcta tgaaattttt tgagggcttg atgtcatacc
    11101 actttttaca aaatgcactg ataacggctg tagtcattgg tatcgtctca ggtgctgtag
    11161 ggtgttttat tatccttagg tcaatgtctc ttatgggtga tgccatctca cacgctgttt
    11221 taccaggggt tgctttgtca tttattttag gagtcaattt ttttattgga gcgattattt
    11281 ttgggttatt agcttctgtt attattactt atatcaagga aaactctgtc attaaaggag
    11341 atacggctat tggtatcacc tttagctctt ttttggcact gggagtcatc ttgatagggg
    11401 tagccaatag ttcgacggac ttatttcata ttttgtttgg gaatatttta gctgtgcaag
    11461 atagcgataa gtggattact attggtgttt cgatttttgt tttagtggtt attagtctct
    11521 ttttcaaaga actattatta acatcatttg atcctatctt agccaaatca atgggtgtga
    11581 aggtcaatgc gtatcattat ttgttgatgg tcctattaac tttagtggct gttacggcga
    11641 tgcaaagcgt gggtactatc ttgattgttg ccttattgat tacgccagca gcgacagctt
    11701 atttatatgc taatagctta aaagtaatgt tagtgatgtc atctttacta ggcgctttag
    11761 catcggtttt agggctttat ttgggctata cctttaatgt tgccgcaggg tcaagtatcg
    11821 tactgacttc tgcgatgatg tttttgatca gtttctttgt ttcacccaag caaggctacc
    11881 ttaaaagatg gatgcaaaaa aaagaaaaaa caccttaaac ttttcgcttt aaacacctta
    11941 gtggtttgca aatagtagca aataacatca aagggtgttt tttatatgtc atcaaaagac
    12001 cataaaaaac cttatcaaac caaggtattt agcctattat ctgataaggt ttttttaagc
    12061 atgatttagt ttttaaagcg ataatctttg acaatgtcaa ttgaggtaat cgtaatgtct
    12121 tgttctggtt tatcattttg gttgatagaa gtagcggcaa ttttatcgac aacatccata
    12181 ccatcaatga cttgaccaaa tacagtataa ccgccatcta agcttggatt gccgccatgc
    12241 tcataggcag agatgatagg ttttgggtag ttggtacttg ataatccctt gctttgattt
    12301 ttcttgtttt ggttgatata aaattggcta ccgttagtat tagcaccagc atttgccatg
    12361 gcaagagcac cgcgaatatg atataaaaat ggagagattt cgttgacaaa gccattgcca
    12421 gcatcttttt taggatcctt gcctttccaa atcgattcac cacctgtgcc atctcctttg
    12481 gggtcacctg attgaatcat aaagtcgttg atcacacgat ggaaggtaag gttatcatag
    12541 tagccttttt tagcatgggt gaggaagttc tcaacagcta agggagcata ttttgggaat
    12601 aacttaaggg taatgtcccc ttgacttgtc ctcattacga ccttagcttc ttctttgccg
    12661 acttctttgg ttagttgtgg aaattggcta gcatcagctt tgagagcttt ttgaatgctt
    12721 tcttcatagg ctttagaagc tgcttcgctt tcttctttgg cagttttttc atcaatgtat
    12781 ttatcgcctt ttatagcgcg gtcaacagat tcacaagcac tcaaactaag taaactaatg
    12841 gctactaaac tgagagataa taattttttc atgtctactc ctaagaatga ttttctttat
    12901 tttatcataa aatgcaacag aaaatagtag taaagagtta aaaacgccta tttttgtgtt
    12961 ttatgatcta accttggtta aaaggctgct acgactgtta ttatagcaca agatgctata
    13021 gaaaaactta aaacaccttg gttttttaga gctatttcct aatagttaaa aaatggtata
    13081 atagtgttat ggttaaaaga aatcaaagaa aaaaaagtgc gcctaagaag cgtttaacaa
    13141 aagcagaagt ggaaaagcaa cgtgccatta agaggatgat tttatctgtt ttaatggctt
    13201 tgttgcttat ttttgcgatg cttcgcttgg gcgtctttgg tgtgacaact tataatatga
    13261 ttcgtttttt ggtgggtagt ttagcctatc cgtttatgtt tgcctggtta atttacttat
    13321 tttgttttaa atggttacgg caaaaagatg gaatgattgc gggagttgtt attgcctttt
    13381 tgggattgct agttgagtgg cacgccttct tatttgcgat gcctaggatg cttgatcaag
    13441 atatttttct aggcactgct cggctaatta caagagattt actagccctg cgcgtgactg
    13501 aatttgttgg tggaggaatg cttggtgcat tactttataa acccatagcc tttttatttt
    13561 cgaatatcgg ctcttacttt attggttttt tgtttatctt attaggatta tttctaatga
    13621 cgccttggga tatctatgat gtgagtcact ttgtgaaaga ggctgttgat aaactagcag
    13681 tggcttatca ggaaaataaa gaaaagcggt tcatcaaacg agaagagcac cgcttacaag
    13741 ctgaaaaaga ggctttagag aaacaagcac aagaggaaga aaaacgtcta gcagaattga
    13801 cggttgatcc tgagacggga gagattgttg aggatagtca gtctcaagtc agctatgatt
    13861 tggctgagga tatgacaaaa gaaccagaga ttcttgctta tgatagtcac ctcaaagacg
    13921 atgagacaag cttatttgat caagaagact tggcttacgc tcatgaggaa attggggctt
    13981 atgactcttt atctgcttta gcatcttctg aagatgagat ggatatggat gaacctgttg
    14041 aagttgattt cacaccaaaa acccatcttc tttataaact tccaacgata gatttatttg
    14101 caccagataa gcccaaaaac cagtccaaag agaaaaactt agtccgcaaa aatatcaagg
    14161 tgcttgaaga taccttccaa agttttggga ttgatgtcaa ggtagagcga gcagaaatcg
    14221 ggccttccgt caccaaatat gaaatcaagc cagctgtcgg ggtccgagtt aatcgtattt
    14281 caaatcttgc tgatgatttg gcacttgctc tggctgctaa agatgttcgt attgaagcac
    14341 caattcctgg aaaatcctta ataggtattg aagttcctaa ctcagaaatt gcaacggttt
    14401 ctttccgcga actttgggaa caatctgatg ccaatcctga aaacctttta gaagtaccac
    14461 taggtaaagc tgttaatggc aatgcgcgca gttttaactt agctagaatg ccgcatcttt
    14521 tggtagctgg ttcaactggt tcaggtaaat ctgtggcagt taatggcatt atttcaagta
    14581 ttttgatgaa ggcacgtcca gatcaagtta agtttatgat gattgacccc aaaatggttg
    14641 aattatctgt ttataatgat attccacatt tattaatccc agttgtaacc aatccgcgta
    14701 aagccagtaa ggcactccaa aaagtggttg atgagatgga aaaccgctac gagttattta
    14761 gtaaaattgg tgtgcgtaat atagcaggtt ataatacaaa ggttgaagag tttaatgctt
    14821 cttctgagca aaaacaaata ccgttgcctt taatcgttgt cattgtagat gaattggctg
    14881 acttgatgat ggtggctagt aaagaagttg aagatgctat tattcgtttg ggacaaaaag
    14941 cacgtgctgc aggtatccat atgattcttg caactcaacg tccctctgta gatgttattt
    15001 ctggtttgat taaagcaaat gttccgtcgc gtatggcatt tgctgtttca agtggtactg
    15061 atagccgtac gatccttgat gaaaatggtg ctgaaaagct cttgggacgg ggtgatatgc
    15121 tctttaagcc gattgatgaa aatcaccctg ttcgcttgca agggtctttc atctcagatg
    15181 atgatgtgga gcgtattgtc aactttatca aagaccaagc tgaagcggat tatgatgacg
    15241 cctttgatcc tggtgaagtt agcgacaatg atcctggttt ttctggaaat ggcggagctg
    15301 ctgaaggtga tcctcttttt gaagaagcaa aagccttggt cttagaaaca caaaaagcta
    15361 gtgcctcaat gattcaaagg cgtttgtcgg ttggatttaa tcgtgccacg cgcttgatgg
    15421 atgaattgga agaagcaggt gttatcggtc cagcagaagg aaccaagcct cgtaaggtat
    15481 tgcagacaaa ctaaaagaac ctgacgtctc atgatgagat gttaggttct ttttttgtga
    15541 tgtcaagcca gttgatgaga tgcctatgct aatgtaaaaa taaagataac tagaatcagg
    15601 tagaaaataa cggtgactaa aaagccaact cgccaaaata gcttcataaa gcgatggtaa
    15661 gaaaacgact gagttcggat gactagcagt agtgaaatga cgatggctag cagcgacatg
    15721 ataataaggt aataaggcag taaattatgg gtgaaaaatt ttcctgagac aagcacaatt
    15781 tctatcgcaa aaagtaaaag tgcgacatct ggaaatttaa atcccttttt attaagttta
    15841 aataaagagg ttacaatcat ggcaaaaaga ggcgttacta tcaagaaagc taaggcagtt
    15901 aatttataga tcatcatagg tctattttat actcaaatcc cttacttgta aactgatact
    15961 tacctgttgt gatagaaaat gatctttgag agggaaagcg tctacttcaa tttaaacatt
    16021 gcaaaaggca tcagctattt taacaaaaag acttgattta ttttggcaat aagctataat
    16081 ggtaaggtat gcaatattgc atatctgtgg gaggtaaaaa tctcaattac cgccaaaacc
    16141 acaacaggag gatttttaaa atggctaaaa aagtcgaaaa acttgtaaaa cttcaaatcc
    16201 ctgctggtaa agctacacca gctccaccag ttggaccagc tcttggtcaa gcaggtatca
    16261 acatcatggg cttcactaaa gaatttaacg ctcgtacagc tgatcaagct ggtatgatca
    16321 tcccagttgt tatctcagtt tatgaagaca aatcatttga tttcatcact aaaacaccac
    16381 cagctgctgt tcttttgaaa aaagctgcag gtgttgaaaa aggatcaggt acacctaaca
    16441 ctactaaggt tgcgacagtt actcgtgcac aagtacaaga aattgctgaa actaagatgc
    16501 cagatttgaa cgctgcaaac attgaagctg caatgcgtat gatcgaaggt actgctcgtt
    16561 ctatgggatt cactgttact gactagtcag tcaagaaccc ctattacccg cacgacttca
    16621 tcataagaga tgagtgacgt gggagatttt aaatcaaatc gatatgacca cattacaagg
    16681 agaattttaa aatggctaaa aaaagcaaac aaatgcgtgc tgcacttgaa aaagtagata
    16741 gcacaaaagc gtacagtgta gaagaagctg tagcattagt aaaagaaacg aacttcgcaa
    16801 aatttgatgc gtctgttgaa gttgcttaca acttgaacat tgacgttcgt aaagcagacc
    16861 aacaaatccg tggcgcaatg gtattgccaa acggaactgg taaaacacaa cgtgttcttg
    16921 tttttgcacg tggtgcaaaa gctgaagaag caaaagcagc tggtgcagac ttcgtaggtg
    16981 aagacgacct tgttgcaaaa atcaatggtg gatggcttga ctttgacgtt gttatcgcaa
    17041 cgccagacat gatggctatc gtaggtcgtc ttggacgtgt ccttggacct cgtaacttga
    17101 tgccaaaccc taaaactggt acagtaacga tggatgttgc taaagccgtt gaagagtcta
    17161 aaggtggtaa aatcacttac cgtgctgaca aagcaggtaa tgttcaagct cttattggta
    17221 aagtttcatt tgatgctgac aaattggttg aaaacttcaa agccttccac gatgtaatgg
    17281 ctaaagctaa acctgcaaca gctaaaggaa cttacatggc aaacgtctca atcacatcaa
    17341 cacaaggtgt tggtatcaag gttgatccta actcacttta agaaaaaaat tagggcatcg
    17401 cctaatcata gcaaaaagac aaatttctga agacaaatct tcactagagg tttgtctttt
    17461 ttatggttta aaatgggatg caaacaaaaa agtgtaggcg ataataccat gaaaggagtt
    17521 gattctctta atgagatgtg gtgttaacac atcatgagct cacaaaaaat gataaaggaa
    17581 gtatcggtgg tgttaaaaaa agggatttta attgacattt attagcctct aaaatgtcct
    17641 ttccctattc ataattgagg gtttgtggta aaatagtaag gacaaactaa ggagaacata
    17701 gtagtggaac ctaaatatca acgtatatta attaagttat ctggtgaagc attagcaggt
    17761 gaaaaaggag ttggcattga cattccaact gttcaggcta ttgcaaaaga aattgccgaa
    17821 gttcatgtgt cgggagtaca gatcgctctt gttattggtg gaggcaatct ctggcgtgga
    17881 gagcctgcag cagacgctgg aatggaccgt gttcaggctg attacactgg catgcttgga
    17941 acggtgatga atgccctagt catggctgat agtcttcaac attatggtgt tgatacccgt
    18001 gtgcaaacag ctattcctat gcaaaatgta gcagaacctt atattcgcgg acgtgccttg
    18061 cgccatctag aaaaaaatcg gattgttgtt tttggagctg gtattggctc gccttatttt
    18121 tcaacagaca ccaccgcggc actgcgtgct gctgagattg aagcagatgc tattttaatg
    18181 gcaaaaaatg gtgtggatgg ggtctataat gctgatccta aaaaagatgc taatgcggtt
    18241 aaatttgatg aattaacaca tggtgaagtg atcaaacgtg gtctcaaaat catggatgca
    18301 acggcatcga ccttatcgat ggataacgat attgatttgg tggttttcaa tatgaatgaa
    18361 gctggcaata ttcaacgtgt tgtctttgga gaacatattg gaactactgt atcaaataaa
    18421 gtttgtgact aaaagttaga aaagaaagag agtaattatc atggcaaatg caattattga
    18481 aactgcaaaa gaacgttttg cacaatccca tcagtcttta tcacgtgaat atgccagcat
    18541 tcgtgcgggt cgtgccaacg ctagtctttt agaccgtatc caagttgatt attacggggc
    18601 accgacacca ttaaatcaat tggcttcaat cactgtacca gaagcgcgtg tcttattgat
    18661 ctcgcctttt gataaatctt ctatcaaaga tatcgagcgc gctcttaacg catcagattt
    18721 aggcattaca cctgctaacg atggctctgt cattcgtttg gttattccag ctttgaccga
    18781 agaaacacgt aaagaattag ccaaagaagt gaaaaaagtt ggtgaaaatg ctaaaattgc
    18841 aatccgtaat atccgccgcg atgctatgga tgatgctaaa aagcaagaaa aagcaaaaga
    18901 aatcactgaa gatgagttaa aaacacttga gaaagatatc caaaaagcaa ccgatgatgc
    18961 tatcaaagaa attgaccgca tgacggctga aaaagaaaaa gaattgctct cagtgtaaca
    19021 ggattcctac gaaaaataga cgactataga cacaaaaaga taggtgacca tttcagaagc
    19081 gtttggtttt gatttgtccc tatcttttta aagaaagaaa aaagatatga atgatttact
    19141 agcaacagtc attactgggc ttattaaaga agaaaacgct aatgactatt ttatccacaa
    19201 agaagggttt acctttacct tgtctaaagc agaaggagaa cgccagattg gtgatatggt
    19261 cacaggcttt gcctatacag acatagagca aaaggcacgt ctcacaacca aagagatccg
    19321 ttcaacacgg acaagttatg gctgggggga agtgacagag gttcgtcgtg acctaggcgt
    19381 ttttgtcgat acaggaattc caaataaaga aattgtggtt tctttggacg tgttgcctga
    19441 gatgaaagaa ctatggccta aaaaagggga taaactttac attcgtttgg atgtggataa
    19501 aaaagaccgt atttggggac ttccagcaga gcctgaggtc tttcaaaaga tggctagccc
    19561 tgcttacaat aacatgcaaa atcaacattg gccagcaatt gtttaccgcc taaaattaac
    19621 aggaacgttt gtttacctgc ctgaaaacaa catgttgggc tttattcact caagtgaacg
    19681 ctacgcagaa ccgcgcttag gtcaggtttt agatgcccgt gtgattgggt ttagagaggt
    19741 cgatcgtacc ttgaatttat cgttgaaacc acgttctttt gagatgttgg agaatgacgc
    19801 tcagatgatt gttacttatc tggaagccaa tggtggtttc atgactttaa atgacaaatc
    19861 aagtccagaa gagattaaag caagctttgg tatttcaaaa ggccagttta aaaaagcctt
    19921 gggtggattg atgaaagcta aacgtatcaa acaagacgcc acaggaactg aattaatagg
    19981 ataaaaatgc tttgccactg gcaaagtttt ttgttacaat gaagaccatt aagtgaagaa
    20041 aaaggggata tagagaatgg aaagagccat ttttgcagga ggttgtttct ggtgtatggt
    20101 gcaacctttt gaggagcaag caggtatttt gtcagtcaga agtggctaca caggtgggca
    20161 tctcccaaat cctagctatg aacaagtttg tgccaaaaca acaggacata cagaagctgt
    20221 tgaaattatt tttgacccta agcaaatcgc ttataaagat ttggtagagc tctattggac
    20281 gcagacggat ccaacagacg cctttgggca atttgaagat cgtggggaca actaccgtcc
    20341 tgtcatttac tacacaacgg aaagacaaaa agaaattgca gagcaatcaa aagcaaactt
    20401 gcaagcttca ggacgttttg accaacccat tgtcaccact attgaaccag cagagccatt
    20461 ttatctcgca gaggattatc accaaggatt ttataaaaag aatcccaagc ggtatgcaca
    20521 aagcagtgct attcgtcatc agtttttaga ggagaattgg tcatgagaaa atcattttac
    20581 agctggctga tgacacaacg taaccctaaa tcaaatgaac ctgtggcgat tttagcagat
    20641 ttggtgtttg atgacaccac ttttccaaag cataccaatg attttgaact cattagtcgg
    20701 tatctagaag atcaagctag tttttccttt aatttaggtc aatttgatga aatctgggaa
    20761 gattatctag cacattaata cgtggataaa aaagagtcat atcgactctt ttgaagggtt
    20821 atcacgttat tattacaaaa tgacatcact ttctttcaaa aaaatgacaa gctacttttt
    20881 ttcttaaaaa tatgctatga tatagcttag aaaaaaataa aaaggaagca gagatgatta
    20941 ttactaaaaa gagcttattt gtgacaagtg tcgctttgtc gttagtacct ttggcgacag
    21001 cgcaggcaca agagtggaca ccacgatcgg ttacagaaat caagtctgaa ctcgtcctag
    21061 ttgataatgt ttttacttat actgtaaaat acggtgacac tttaagcaca attgctgaag
    21121 caatggggat tgatgtgcat gtcttaggag atattaatca tattgctaat attgacctaa
    21181 tttttccaga cacgatccta acagcaaact acaatcaaca cggtcaggca acgaatttga
    21241 cggttcaagc acctgcttct agtccagcta gcgttagtca tgtacctagc agtgagccat
    21301 taccccaagc atctgccacc tctcaaccga ctgttcctat ggcaccacct gcgacaccat
    21361 ctgatgtccc aacgacacca ttcgcatctg caaagccaga tagttctgtg acagcgtcat
    21421 ctgagctcac atcgtcaacg aatgatgttt cgactgagtt gtctagcgaa tcacaaaagc
    21481 agccagaagt accacaagaa gcagttccaa ctcctaaagc agctgaaacg actgaagtcg
    21541 aacctaagac agacatctca gaagccccaa cttcagctaa taggcctgta cctaacgaga
    21601 gtgcttcaga agaagtttct tctgcggccc cagcacaagc cccagcagaa aaagaagaaa
    21661 cctctgcgcc agcagcacaa aaagctgtag ctgacaccac aagtgttgca acctcaaatg
    21721 gcctttctta cgctccaaac catgcctaca atccaatgaa tgcagggctt caaccacaaa
    21781 cagcagcctt caaagaagaa gtggcttctg cctttggtat tacgtcattt agtggttacc
    21841 gtccaggtga tccaggagat catggtaaag gtttggccat tgattttatg gtgcctgaaa
    21901 attctgctct tggtgatcaa gttgctcaat atgccattga ccatatggca gagcgtggta
    21961 tttcatacgt tatttggaaa cagcgattct atgcgccatt tgcaagtatt tacggaccag
    22021 cctacacatg gaaccccatg ccagatcgcg gcagtattac agaaaaccat tatgatcatg
    22081 ttcatgtctc ctttaatgct taattcatgc tattaaaaaa actcacgttt aggcgtgagt
    22141 tttttgacaa ttctgacaga gtaagcccta taagaggctg agatacgctt ttaatacctg
    22201 ttgtttttct caccctttag acagttttaa cgttatgtct aaaagagatg gtttttatta
    22261 gcgcaaatat gggtttaggg gtaaaataaa ggtaattaac ctgatatgat catgataaag
    22321 gtagaagaat acctattatt tcgtgacaac tgagcaatct gccttacctt tggagtgatg
    22381 atatcacttt gcttgactat tcaaggaggt cttgaaatgt attatactag tggtaattac
    22441 gaagcttttg caacacctcg aaaacctgaa ggggtagatc agaaatcagc ttatattgtt
    22501 ggtactggtt tagctggttt agcagcagct gttttcctta ttcgcgatgg gcatatggct
    22561 ggggaacgca ttcatctgtt tgaggaattg cctttagcag gtggttcttt agatggtatt
    22621 gaaaagcctc atcttggttt tgtgacccgt ggtggtcgtg agatggaaaa tcattttgag
    22681 tgtatgtggg acatgtatcg gtctattccc tcactggaaa ttcccggtgc gtcttattta
    22741 gatgaatttt attggttgga taaggatgat cctaactcat ccaactgtcg tttgattcac
    22801 aagagaggaa atcgtgtgga tgatgacggc cagtatacgc tcggtaaaca gtcaaaagaa
    22861 ttaatccatt taatcatgaa gacagaagaa tctctaggag accaaaccat tgaagagttc
    22921 ttctcagaag atttctttaa gagtaatttt tgggtgtatt gggcaaccat gtttgctttt
    22981 gaaaaatggc attctgctgt agaaatgcga cgctatgcga tgaggtttat ccaccatatt
    23041 gatggtttgc cagatttcac ctccctcaag ttcaacaaat ataaccaata tgactctatg
    23101 gtcaaaccga ttattgctta cctagaatca cacgacgttg acatccaatt tgacacaaaa
    23161 gtcactgata ttcaggtgga gcaaacagct ggtaaaaagg tagcaaaaac catccatatg
    23221 acggtgtctg gggaggctaa ggcgattgag ctaacacctg atgatttggt ttttgtgacc
    23281 aatggttcta ttactgaaag cagcacatac ggtagtcatc acgaagtggc taagccaacc
    23341 aaagcgttag gtggttcttg gaatttatgg gaaaatctag ctgctcaatc agatgatttt
    23401 ggtcatccta aagtgtttta ccaggacttg cctgctgaaa gctggtttgt gtctgctaca
    23461 gcaaccataa aacacccagc tatcgagcct tatatagaac gtttgaccca ccgtgacttg
    23521 cacgatggca aagtgaacac tggcggcatc atcactatta cagattctaa ctggatgatg
    23581 agctttgcca ttcaccgtca acctcatttt aaagaacaaa aagaaaatga gaccactgtc
    23641 tggatttacg gtctttattc caatagtgag ggcaattacg tccacaagaa aattgaggag
    23701 tgtacaggtc aagaaatcac agaagaatgg ttgtaccacc ttggggtacc tgttgataaa
    23761 atcaaggact tagcgagtca ggactatatc aatacagttc ctgtttacat gccttatatt
    23821 acgagttact ttatgccacg cgtcaaagga gaccgtccga aagttatccc agatggttca
    23881 gtcaacttgg cctttattgg taactttgcg gaatctccat ctcgagatac ggtctttacg
    23941 actgagtatt ctattcgtac tgccatggaa gcagtgtata gcttcttgaa tgtggaacga
    24001 ggcatcccag aagtctttaa ttcagcctat gatattcgtg aattgctcaa agccttttat
    24061 taccttaatg ataaaaaggc aatcaaggat atggatttgc caattcctgc actgattgag
    24121 aaaatcggac ataaaaaaat caaggatacc tttatcgaag aattgctcaa agatgctaat
    24181 cttatgtaac atgccttaga gtagttaaag ctgataagtt taggcttatc agcttttttg
    24241 atgatttata gaaaagtgat aaaggaaaag tctgacgtga acgtcacata agaatttaaa
    24301 atttgttata atgtaagcaa taaagatgaa agaaagaggg actctatttg caagagtatt
    24361 ctattgacat taccttaacc catcctgatg atgttttagc tctttttggt agcaacgagc
    24421 gtcatttgaa gctaatagaa gctcatttag gagttattgt tcacgcaaga accgagcgag
    24481 tccaagtgat tggtgatgat gaagaagctg tggaattggc tcgattgacc atcaaagccc
    24541 tactggttct agtgggtcgt ggcatggtgg tcaacacatc agatgtggtg acagctttat
    24601 ctatggcaga atctcatcag attgaccaat ttatggcgct ttatgaagaa gaaatcatta
    24661 aagataacta tggtaaggca atacgagtca aaacattagg tcaaaaaacc tatgttgata
    24721 gtgtcaaacg tcatgatgtg gtttttggtg taggacctgc agggacaggt aaaaccttct
    24781 tagctgttac gcttgcagtc actgctctga aaagaggaca ggtaaagcgt attattttga
    24841 cgcgtcctgc ggtagaagct ggcgaaagcc taggtttttt accaggcgat ttgaaagaaa
    24901 aggtggatcc ttacctcaga cctgtttacg atgcccttta tcacatttta ggcaaggaac
    24961 aaaccacacg cctgatggaa cgagatgtga ttgagattgc tccgttagct tatatgcgag
    25021 gtcgtactct ggatgacgct tttgttatct tagatgaggc tcaaaacacg accatcatgc
    25081 aaatgaagat gtttttgacc cgtcttggtt ttaattctaa aatgattgtc aatggagata
    25141 cgagtcagat tgacctccct cgaaatgtca agtctggttt gattgatgct actcagaaat
    25201 tacaaggcat taaacagatt gattttgtct atttctctgc taaggatgtt gtccgacatc
    25261 ctgttgtggc tgatattatc aaggcttatg aaacgtcctc agaggagatg aaggacttac
    25321 taaaacctaa ggctagtgac gttgaagctg attaccagcc aggcttgaca aaataccctg
    25381 tgatcggtca agagcactaa gacttttacg atgatgttta atatggcctt tttaaggagg
    25441 attctatgga tgatttaacc aaagctatta tggcagatga ggctaatctc tcctataccg
    25501 aaagaggtat ttttccttta tatgatgctc ctcaaacagc taggattatt attgttggcc
    25561 aagctcctgg aatagtagct caaggaacca aactttactg gaatgatcgt agtggcatcc
    25621 gcttacgaga ctggctaggt gtggacaatg acacctttta ccactcagga ttgtttggca
    25681 ttatccctat ggatttctat tatcctggta agggaaaatc cggtgactta ccacctagag
    25741 aaggttttgc ggctaagtgg cacccgccat taagagcatt gatgccagaa gttgaattga
    25801 cgattttggt tggtcgctat gctcaggact tttaccttgg taacaaggct tacaaaacct
    25861 taactgaaac agtccgtcac tttgaggatt acttgccaga ctattttcca cttgttcatc
    25921 cttctccaag aaaccaattg tggttagcta agaacccctg gtttgagcag gatttattac
    25981 ctatcttgca aaaacgtgtg gaggcaattc taaccaaata ggccctgatt tacctagcat
    26041 tcccattttt aaggggcttg tggtataatg ggtaaataac aaatgctgat tgaaaggata
    26101 agggaaatta tctgtcagat aataatgggt gcccaacata ccaaatgagg tgggcagccc
    26161 ttccctaaca attaacctta tgtatatcga gatgattgac gaaacgggac aagtttcgca
    26221 agagattatg gagcaaacac ttgatttgct caattttgct gctcaaaaga cgggcaaaga
    26281 agaaaaagaa atgtctgtga cctttgtgac caatgaaaga agtcatgaac tcaacttaga
    26341 ataccgagat actgatcgtc caacagatgt gatttctcta gaatataagc cagaaacgcc
    26401 tattttattt agtcaagagg atttggctgc tgatcctagt ttggcagaga tgatggcaga
    26461 gtttgatgct tatattggcg aattgtttat ttctattgac aaggcgcgtg agcagtccca
    26521 agaatatggg cactcttttg agcgtgaaat gggatttttg gctgttcatg gattcttaca
    26581 tattaatggg tatgatcact acacccttga agaagaaaaa gagatgttta ccttacagga
    26641 agagattttg actgcttatg gccttacacg acaataatac caccaaacgg aaatggaaaa
    26701 acagaaccat tacctcaagc ttggagtttg ccttgacagg ggttttcaca gcttttaagg
    26761 aagaacgcaa tctcagaagc caccttttgt ctgcctgttt agcttgtgtt gctggtttat
    26821 tttttagcat ttcagccatc gaatggttat ttttacttct agccattttt ttggtcatta
    26881 ccttagaaat tgtcaattct gctattgaaa atgtagttga tttggctagt gactatcact
    26941 tttctatgct agctaaaaat gccaaggata tggcagcagg tgctgttttg atgatttctg
    27001 gctatgctgt cttgacagga cttattattt ttattcctaa aatttggaat attttcgttc
    27061 attaaaaaga aaggaatggg ctatcctatg tgatgatggc ataggagaat aaggaaagga
    27121 caagacggtt aagcttagtg acaagcttga tctgaagccc taccgttaaa gcccgtaatt
    27181 atctatgttt aaatcaggct ttgtagccat tcttggtcgt ccaaatgttg gcaaatccac
    27241 ctttttaaat cacgtcatgg ggcaaaaaat tgccatcatg agtgacaagg ctcagaccac
    27301 ccgtaataaa attatgggca tttacaccac agaaacagaa caaattgtct ttattgatac
    27361 gccaggtatt cataaaccaa agaccgccct tggtgatttt atggttgaat ctgcttatag
    27421 tactttacgt gaagttgaga cggttctttt catggttcct gccgatgaaa agcgtggtaa
    27481 gggtgacgac atgattatcg agcggctaaa agcagctaag attcccgtta ttttagtcat
    27541 caacaaaatt gataaggttc atccagacca acttttggaa cagattgatg acttccatag
    27601 ccaaatggat tttaaagaag tggtcccaat ttctgcccta gaaggtaata atgtgccaac
    27661 tttgatcaag ttattaactg ataatcttga agaaggtttc caatattttc cagaagatca
    27721 gattactgat catccagaac gtttccttgt gtcagaaatg gttcgtgaga aggtactgca
    27781 tttgacgcaa caagaggttc cacattccgt agctgttgtt gtcgaatcca tgaagcgaga
    27841 tgaagagact gataaagtgc atatccgtgc aaccattatg gtggagcgcg atagccaaaa
    27901 aggcatcatc attggaaaac aaggagccat gctcaaaaaa atcggaaaaa tggcgcgccg
    27961 tgatattgaa ctcatgttag gcgacaaggt ctacttagaa acgtgggtca aagtcaagaa
    28021 aaactggcgg gataaaaagc ttgatttagc tgattttggc tataatgaga aagaatacta
    28081 acatagtaaa gcgagatcat atgccacaag actacatttc ttatatccgc tccaaagttg
    28141 ggcatgataa gattattctt aactttgctg gtgggatttt gaccaatgat gacggcaagg
    28201 tgctgatgca gctgcgtggt gataagaaaa cctggactat tcctggtggc actatggaac
    28261 tgggtgagag ctctttagag acttgcaaac gtgagttctt agaggaaacc ggaattgagg
    28321 ttgaagctgt tcgcttactg aatgtttaca ctcattttga ggaagtttat cccaacggtg
    28381 atgctgttca gactattgtc tttatctatg aactaacggc tgtttctgat atggctattg
    28441 ataactttca taatgaggaa acgctcaagt tacaattttt ctctcatgag gagatagcag
    28501 agttagagag tgtctcagcc aaacatcgcc tgatgttaga ggaatatttt agtgatagct
    28561 ttgctatggg gcattaagaa ctctctagga cagttggagt atttttggag attggcttgt
    28621 taaaaaaatt tttgaaagac gatattcgag agcttatgct ctcttttttt gcttccaatt
    28681 tttctgatta agtctgaata attttgttaa atgttaacag gattttcttt ccaattgata
    28741 ctagcagaca tcaaacattt gttttagagt tatgacgaat tgccacctag ccatgcatga
    28801 cttaggcaag ttgaacctaa ctgtgacaat agacttggga ggggtgacaa gaatcatttc
    28861 ctaaaagaat tgatgaagtg cctatccatt attcgaattg taaagttcgt ctaagtaaag
    28921 ttgaatttgg tctcgaatgg accttaatga tttatgactt gttgtgtcaa taacaccaaa
    28981 aactcgataa tgatttttaa atatttcctc gtaattaata tgatttccga ttagaataat
    29041 atgagcatga ggataacgca tacgtatttc gtttagaagg tcatatactt gcttagattt
    29101 aaaaaagtat gatttatgat aaaataaacc tgacatttgt atttataatc taagtttttc
    29161 ccactaataa tttgagtttt tagaaagtat ctcttgaggt aatagttaat atagatagat
    29221 atcatatatc ctaaaagaga acgatgtctt gtaagtattt tcgaagacta taatattaag
    29281 ttcggagctc gtattcataa aggattcgga gtatgttttg acacacttga tagcttattt
    29341 gactaa
//
LOCUS       DAXWIQ010000020        28154 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000020 DAXWIQ010000000
VERSION     DAXWIQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28154
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.020"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            73..864
                     /locus_tag="ACGEUI_000901"
     CDS             73..864
                     /locus_tag="ACGEUI_000901"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HFF8165746.1"
                     /translation="MQFKHIIFDVVDDLATLTLNRPEVSNGFNIPICQEILVALAEVK
                     RDTSVRFLLIKAVGKVFSVGGDLVEMQEAVAKDNVQSLVKIAELVQEISFAIKHLPKP
                     VILCADGAVAGAAFNIALAVDFCIASTQTKFIQAFVNVGLAPDAGGLFLLTRAVGLNR
                     ATHLVMTGEGITADKGLDYGFVYRTAESDKLDKVCLQLLKRLRRGSSNSYAGMKSLVW
                     QSFFTGWEDYAKAELAIQEELAFKEDFKEGVIAFGERRRPNFQGK"
     gene            941..1375
                     /locus_tag="ACGEUI_000902"
     CDS             941..1375
                     /locus_tag="ACGEUI_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8165747.1"
                     /translation="MEYDKINQYLVDIFNRILVIEEMSLKTSQFSDVSLKEMHTIEII
                     GKYDQVTPSDIARELMVTLGTVTTSLNKLEAKGYIARTRSRSDRRVVYLSLTKRGRLL
                     DRLHAKFHKNMVGHVIADMSDEEMQALVRGLGNLHQFLEDLV"
     gene            1376..2350
                     /locus_tag="ACGEUI_000903"
     CDS             1376..2350
                     /locus_tag="ACGEUI_000903"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFF8165748.1"
                     /translation="MIFSKISQVAHYVPQQLVTNNDLASIMDTSHEWIFSRTGIAERH
                     ISRDEMTSDLAIQVADQLLTQSGLKADAIDFIIVATISPDATMPSTAAKVQAAIAATS
                     AFAFDMTAACSGFVFALAMADKLIASGAYQNGMVIGAETLSKLVNWQDRATAVLFGDG
                     AGGVLLEASKDKHVLAETLHTDGARCQSLISGETSLSSPYSIGKKAIATIQMDGRAIF
                     DFAIRDVSKSILTLMAQSDITKDDIDYCLLHQANRRILDKIARKIDVPREKFLENMMR
                     YGNTSAASIPILLSEAVQKGQIRLDGTQKILLSGFGGGLTWGSLIVRI"
     gene            2411..2635
                     /locus_tag="ACGEUI_000904"
     CDS             2411..2635
                     /locus_tag="ACGEUI_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFF8165749.1"
                     /translation="MAVFEKVQEIIVEELGKETEEVTLETTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKSK"
     gene            2837..3808
                     /gene="fabK"
                     /locus_tag="ACGEUI_000905"
     CDS             2837..3808
                     /gene="fabK"
                     /locus_tag="ACGEUI_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HFF8165750.1"
                     /translation="MKTRITELLNIDYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDRVKAITDRPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHQAGIIVVPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMSLVRQVVEA
                     VSIPVIAAGGIADGHGAAAAFMLGAEAVQIGTRFVVAKESNAHQNFKDKILAAKDIDT
                     VISAQVVGHPVRSIKNKLTSAYAKAEKAFLIGQKTATDIEEMGAGSLRHAVIEGDVVN
                     GSVMAGQIAGLVRKEESCETILKDIYYGAARVIQNEAKRWQSVSIEK"
     gene            3833..4771
                     /gene="fabD"
                     /locus_tag="ACGEUI_000906"
     CDS             3833..4771
                     /gene="fabD"
                     /locus_tag="ACGEUI_000906"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFF8165751.1"
                     /translation="MTKTAFLFAGQGAQKLGMARDFYDNFAIVRKTFDQASQVLGYDL
                     RRLIDSDELKLNQTSYTQPAILTSSIAIYRVLGLHHVKPDMVAGLSLGEYSALVASGA
                     LSFEDTLSLVAKRGRLMEEAAPQGSGKMVAVMNTDVQVIEEVCQIAAKHGVVAPANYN
                     TPSQIVIGGQTDAVNVAVELLKERGVKRLIPLNVSGPFHTALLEPASRLLAKELERYN
                     FSDFKIPLVGNTEANIMEKDRIPELLARQVMEPVRFYDSVATLVESGITQFIEVGPGK
                     VLTGFVKKIDKNLLCTSVENMVSLRLFLDREDIHGN"
     gene            4761..5495
                     /gene="fabG"
                     /locus_tag="ACGEUI_000907"
     CDS             4761..5495
                     /gene="fabG"
                     /locus_tag="ACGEUI_000907"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFF8165752.1"
                     /translation="MEIKGKNIFITGSTRGIGLAMAHQFASLGANIVLNGRSAISEEL
                     VASFTDYGVTVVTISGDVSEASEAKRMVNEAIESLGSIDVLVNNAGITNDKLMLKMTE
                     EDFERVLKINLTGAFNMTQSVLKPMIKARQGAIINVSSVVGLTGNIGQANYAASKAGM
                     IGFTKSVAREVAARNICVNAIAPGFIESDMTGVLPEKMQEQILSQIPMKRIGKAQEVA
                     HLASFLVEQDYITGQVIAIDGGMTMQ"
     gene            5510..6742
                     /gene="fabF"
                     /locus_tag="ACGEUI_000908"
     CDS             5510..6742
                     /gene="fabF"
                     /locus_tag="ACGEUI_000908"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFF8165753.1"
                     /translation="MTFKRVVVTGYGLTSPIGHDPETFWNNLKAGQIGIGPITKFDTT
                     DYAVKNAAEIQDFPFDKYFVKKDLNRFDRYSLYALYAAKEAINHADLNIEMVDSDRFG
                     VIVASGIGGIAEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMSLKAQGVCKSI
                     NTACASSNDAIGDAFRAIKFGTQDVMIVGGSEAAITKFAIAGFQSLTALSTTEDPSRS
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAQERGATILAEIVGYGNTCDAYHMTSPNPEG
                     LGARKAIHLALQEAGIEASAINYVNAHGTSTPANEKGESQAIVAVLGKDVPVSSTKSF
                     TGHLLGAAGAIEAIATIEAMRHNYVPMTAGTQALSEDIEANVIFGEGKETAINYAISN
                     TFGFGGHNAVLAFKCWEE"
     gene            6744..7244
                     /gene="accB"
                     /locus_tag="ACGEUI_000909"
     CDS             6744..7244
                     /gene="accB"
                     /locus_tag="ACGEUI_000909"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFF8165754.1"
                     /translation="MNIQEIKDLMAQFDTSSLREFLFKTNEGELIFSKNEQHLNASTS
                     NQEHAVPVPQVQLVPNPTASEASSPASVKDVPVEEQPQAESFVAEGDIVESPLVGVAY
                     LAASPDKPPFVAVGDTVKKGQTLVIIEAMKVMNEVPAPCDGVITEILVSNEDVIEFGQ
                     GLVRIK"
     gene            7241..7663
                     /gene="fabZ"
                     /locus_tag="ACGEUI_000910"
     CDS             7241..7663
                     /gene="fabZ"
                     /locus_tag="ACGEUI_000910"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFF8165755.1"
                     /translation="MMDIREIQAALPHRYPMLLVDRVLEVSDDHIVAIKNVTINEPFF
                     NGHFPHYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFIKRRGTIAVVEARAEVDGKLAASGTLTFACGQ"
     gene            7695..9059
                     /locus_tag="ACGEUI_000911"
     CDS             7695..9059
                     /locus_tag="ACGEUI_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFF8165756.1"
                     /translation="MFKKILIANRGEIAVRIIRAARELGISTVAVYSEADKEALHTIL
                     ADEAICIGPARSKESYLNMNSVLSAAIVTGAQAIHPGFGFLSENSKFATMCEEMNIKF
                     IGPSASVMDKMGDKINARSEMIKAGVPVIPGSDGEVYNAQEALAIANKIGYPVMLKAS
                     AGGGGKGIRKVETEADLEAAFNAASQEALGAFGNGAMYLEKVIYPARHIEVQILGDAY
                     GNIIHLGERDCSLQRNNQKVLEESPSIAIGNTLRHEMGQAAVRAAEAVAYENAGTIEF
                     LLDEDSEKFYFMEMNTRIQVEHPVTEFVTGVDIVKEQIKIAAGQPLAINQEDITITGH
                     AIECRINAENTAFNFAPSPGKITDLYMPSGGVGLRVDSAVYNGYAIPPYYDSMIAKII
                     VHGSNRFDALMKMQRALVELEIEGIITNTDFQLDLISDKRVIAGDYDTSFLMETFLPH
                     YNKD"
     gene            9068..9934
                     /gene="accD"
                     /locus_tag="ACGEUI_000912"
     CDS             9068..9934
                     /gene="accD"
                     /locus_tag="ACGEUI_000912"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFF8165757.1"
                     /translation="MALFRKKDKYIRITPNNSLKGSVSHNVPEVPDELFAKCPACKHM
                     IYKKDLGLAKICPTCSYNFRISAQERLTLTVDEGSFQELFTSIETKDPLRFPGYQEKL
                     QKAKETTGLHEAVLTGKAMVKEQKIALAIMDSHFIMASMGTVVGEKITRLFELAIEEN
                     LPVVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNAGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQSLVGFAGRRVIETTVRENLPDDFQKAEFLQDHGFVDAIVKRTELRDK
                     IAHLVAFHGGGQ"
     gene            9931..10701
                     /locus_tag="ACGEUI_000913"
     CDS             9931..10701
                     /locus_tag="ACGEUI_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFF8165758.1"
                     /translation="MTDVSRILKEARDQGRLTTLDYANLIFDDFMELHGDRHFSDDGA
                     IVGGLAYLAGQPVTVIGIQKGKNLQDNLARNFGQPNPEGYRKALRLMKQAEKFGRPVV
                     TFINTAGAYPGVGAEERGQGEAIAKNLMEMSDLKVPIIAIIIGEGGSGGALALAVADQ
                     VWMLENTMYAVLSPEGFASILWKDGSRATEAAELMKITAGELYKMGIVDRIIPEHGYF
                     SSEIVDIIKANLIEQITSLQAKPLDQLLDERYQRFRKY"
     gene            complement(10923..12200)
                     /gene="serS"
                     /locus_tag="ACGEUI_000914"
     CDS             complement(10923..12200)
                     /gene="serS"
                     /locus_tag="ACGEUI_000914"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFF8165759.1"
                     /translation="MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRALLVQ
                     SEELKAERNIASAAIAQAKRQKEDATQQIADMQKVSADIKTIDNQLVAIDQQVTDIIT
                     VLPNTPHDSVPVGADEEDNVEIRRWGTPRDFDFEVKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRGEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLGLPYRVISLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGGETVISPK"
     gene            complement(12592..12951)
                     /locus_tag="ACGEUI_000915"
     CDS             complement(12592..12951)
                     /locus_tag="ACGEUI_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFF8165760.1"
                     /translation="MAQSLNTSVEYKTKAVSYLGMGGKVGHILLGDKALEFYNDKNVN
                     DYIQIPWTAINHIGANVSRKKVSRHFEIFTDQGKFLFASGDSGKILKITRQHIGNEKV
                     ITLPTLMQTFINKFFRH"
     gene            complement(13065..13976)
                     /locus_tag="ACGEUI_000916"
     CDS             complement(13065..13976)
                     /locus_tag="ACGEUI_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8165761.1"
                     /translation="MTEQIKLTKSDRQRVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTSPEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEERANGTPIDDKAIQGVKI
                     GMMGPLAGIGDPVFWFTIRPILGALGASLASTGNIVGPLLFFFGWNLIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSINFTIDLPGKQLSDGA
                     YVVFPDGAVKGAELKTILANAIGGMSLDKVQAQTLQGQLDSLIPGLAGLLLTFLCMWL
                     LKKKVSPIAIIIGLFAFGILAHLAGIM"
     gene            complement(13993..14802)
                     /locus_tag="ACGEUI_000917"
     CDS             complement(13993..14802)
                     /locus_tag="ACGEUI_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFF8165762.1"
                     /translation="MSDISIISAILVVIIAFFAGLEGILDQFQMHQPLVACTLIGLVT
                     GHLEAGVILGGTLQMLALGWANIGAAVAPDAALASVAAAIIMVKSGDFTQKGITFAYS
                     TAIPLAVAGLFLTMIVRTLSTALVHAGDKAAAEGNFAGIERFHFIALLLQGLRIAVPA
                     ALLVAVPTSAVQSVLNAMPNWLNEGMQIGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAAISQLTLIAMGVIGVAIAFIYLNLSKKGGNGGNAAGSADPIGDILEDY"
     gene            complement(14891..15883)
                     /locus_tag="ACGEUI_000918"
     CDS             complement(14891..15883)
                     /locus_tag="ACGEUI_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8165763.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIQQFDADDEILVLADLWSGSPFNQASRVAGENPDRKMAIITGLNLPMLIQ
                     AYTERLMDAGAGVEQVAANIIKESKDGIKALPEDLNPVEETAATEKVVNALQGAIPAG
                     TVIGDGKLKINLARVDTRLLHGQVATAWTPASKADRIIVASDEVAQDDLRKQLIKQAA
                     PGGVKANVVPISKLIEASKDPRFGNTHALILFQTPQDALRAVEGGVEINELNVGSMAH
                     STGKTMVNNVLSMDKEDVATFEKLRDLGVTFDVRKVPNDSKKNLFELIQKANIK"
     gene            complement(16235..17047)
                     /locus_tag="ACGEUI_000919"
     CDS             complement(16235..17047)
                     /locus_tag="ACGEUI_000919"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8165764.1"
                     /translation="MTKKLIAIDLDGTLLHHDNTISTYTQKTIKAVQDKGHHVIISTG
                     RPYRMALGYYLQLNLKTPIITFNGALTHMPEQKWAYEHNVTLDKGYLLRLLKYQDDFQ
                     MDFIASEYRKNVYITMTNPESIDPQLFGVDEITQDMALEITKITRNPNALLMQTHHED
                     KYALAKNMRACFKDEIEIDSWGGPLNILEISSKNVNKAYALNYLLGIYNMDKKDLIAF
                     GDEHNDTEMLAFAGTGYAMKNASPVLLPYADQQLNFSNEEDGVAKKLEELFL"
     gene            17311..18771
                     /locus_tag="ACGEUI_000920"
     CDS             17311..18771
                     /locus_tag="ACGEUI_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFF8165765.1"
                     /translation="MEKFFKLSENGTTVSTEIMAGLTTFFAMSYILFVNPSILGAAGM
                     PSNAVFLATIIAAAISTLIMGLFANVPYALAPGMGLNAFFTYTVVFALGFSWQEALAM
                     VFICGLFNIFITVTKFRKSIIKAIPVSLQHAIGGGIGVFVAYLGFKNANIITFSISAE
                     NIVMVNGVEPAKASAKTFADGLLFVDANGGVVPTISSFTDSGVLLAIFGLLLTTALVI
                     RNFRGAILIGIVATTLVGIPLGIVDVSNLNFGISHIGEAWTELGTTFLAAFDGLSSLF
                     SDSSRLPLVFMTIFAFSLSDTFDTIGTFIGTGRRTGIFSQDDENALENSIGFSSKMDR
                     ALFADAIGTSIGALVGTSNTTTYVESAAGIAEGGRTGLTAVSTAVCFLLSILLLPLVG
                     IVPAAATAPALIIVGVMMVSSFLDVNWSKFADALPAFFAAFFMALCYSISYGIAAAFI
                     FYCLVKVVEGKTKDIHPIIWGATFLFIVNFIILTIL"
     gene            18925..19386
                     /gene="tsaE"
                     /locus_tag="ACGEUI_000921"
     CDS             18925..19386
                     /gene="tsaE"
                     /locus_tag="ACGEUI_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFF8165766.1"
                     /translation="MFYSENEYTLKAYGETLGTYLSIGDVIVLSGDLGAGKTTLAKGI
                     AKGMGISQMIKSPTYTIVREYEGRLPLYHLDIYRVGDDPDSIDLDDFLFGNGVTVIEW
                     GELLGEGLLQDYLQITITKRDKGRQLDLLAHGERSRQLLEIMSHGSAKSRY"
     gene            19361..19885
                     /locus_tag="ACGEUI_000922"
     CDS             19361..19885
                     /locus_tag="ACGEUI_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFF8165767.1"
                     /translation="MVQQSLVIEEGVPQDAKAVLSLVTAAAKETDFITGVETILEATP
                     QELSDFLSRSQTSFIDFCLLARLDEKVVGLLNLSGEVLSQGQAEADVFMLVAKTYRGY
                     GIGQLLLEIALDWAEENPYIESLKLDVQVRNTKAIYLYKKYGFRIESMRKNDIKSKNG
                     DDLDVYHMRKSVSQ"
     gene            19894..21168
                     /locus_tag="ACGEUI_000923"
     CDS             19894..21168
                     /locus_tag="ACGEUI_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8165768.1"
                     /translation="MKIGKKIVLMFTAIVLTTVLALGVYLTSAYTFSTGELSKTFKDF
                     STSSNKSDAIKQTRAFSILLMGVDTGSSERASKWEGNSDSMILVTVNPKTKKTTMTSL
                     ERDTLTTLSGPKNNEMNGVEAKLNAAYAAGGAQMAIMTVQDLLNITIDNYVQINMQGL
                     IDLVNAVGGITVTNEFDFPISIAENEPEYQATVAPGTHKINGEQALVYARMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALDSISSYRKILSAVSSNMQTNIEISSRTIPSLLGYRD
                     ALRTIKTYQLKGEDATLSDGGSYQIVTSNHLLEIQNRIRTELGLHKVNQLKTNATVYE
                     NLYGSTKSQTVNNNYDSSGQAPSYSDSHSSYANYSSGVDTGQSASTDQDSTASSHRPA
                     TPSSSSDALAADESSSSGSGSLVPPANINPQT"
     gene            complement(21279..21635)
                     /locus_tag="ACGEUI_000924"
     CDS             complement(21279..21635)
                     /locus_tag="ACGEUI_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HFF8165769.1"
                     /translation="MKVKTVIAVGLLSFTAYKAYQKRCTIKELLSISRQAKDAAQLDL
                     DNIKANLDLIHSQGKVIQNISQDLAHKWRYFNQETQAHLTEIQNRMAKYQEDSELISS
                     ESPTCQTVILTNNHSR"
     gene            complement(21632..22051)
                     /locus_tag="ACGEUI_000925"
     CDS             complement(21632..22051)
                     /locus_tag="ACGEUI_000925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFF8165770.1"
                     /translation="MENCIFCSIIQGDIPSSKVYEDEQVLAFLDISQTTKGHTLVIPK
                     QHVRNLLEMTAETASHLFARIPKIARAIQSATGATAMNIINNNEALAGQTVFHAHVHL
                     VPRYNEEDGISIQYTTHEPDFPVLEKLARQINQEVSS"
     gene            22123..22848
                     /locus_tag="ACGEUI_000926"
     CDS             22123..22848
                     /locus_tag="ACGEUI_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8165771.1"
                     /translation="MLNIKNLTGGYHNIPVLNDVSFSVDNGELVGLIGLNGAGKSTTI
                     NEIIGFLKPYQGSISIDGLTLAENAVAYRQKIGFIPETPSLYEELTLSEHINTVAMAY
                     DIDLEVAQKRAQPFLEMFRLTDKLEWFPVNFSKGMKQKVMIICAFVIDPSLFILDEPF
                     LGLDPLAISDLIQTLEVEKAKGKSILMSTHVLDSAERMCDRFVILHHGQVRAQGTLAD
                     LQEAFGDRSASLNDIYLALTKED"
     gene            22851..23885
                     /locus_tag="ACGEUI_000927"
     CDS             22851..23885
                     /locus_tag="ACGEUI_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8165772.1"
                     /translation="MKALFLKRRQDFQKQQNKYLRYVLNDHFVLVLMFLLGFAMVQYG
                     QLLNHFPTNHLPIQVCLGILIPLLLSMGSIATYLEEADQHFLLPKEEEVISYIKQAER
                     LSFLLWGTLQTAVLLFLYPIFRRLGLSLFIFIILVLILLALKRVVLSRKTRYFLRGNR
                     LDWAKAVAFESNRKQSILKFYSLFTTVKGISTKVKERTYLNPLLKLVKQTPSNLWLSL
                     YARAFLRSSDYLGLFLRLMLLSSLSVFFIHNLYLSVSLALIFNYLVVFQLLSLYYHYD
                     YHYMTSLYPENSRSKKKNMLSFLRGLSFLMLIVNMLCCSSAPKALILIVGMVFIACIY
                     LPYKLKKIID"
     gene            23949..24740
                     /gene="ccrZ"
                     /locus_tag="ACGEUI_000928"
     CDS             23949..24740
                     /gene="ccrZ"
                     /locus_tag="ACGEUI_000928"
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle regulator CcrZ"
                     /protein_id="HFF8165773.1"
                     /translation="MTTTEQELTLTPLRGKSGKAYKGTYPNGECVFIKLNTTPILPAL
                     AKEQIAPQLLWAKRMGNGDMMSAQEWLNGRTLTKEDMNSKQIIHILLRLHKSKKLVNQ
                     LLQLNYKIENPYDLLVDFEQNAPLQIQQNSYLQAIVKELKRSLPEFKSEVATIVHGDI
                     KHSNWVITTSGMIFLVDWDSVRLTDRMYDVAYLLSHYIPRSRWSEWLSYYGYKNNDKV
                     MQKIIWYGQFSHLTQILKCFDKRDMEHVNQEIYALRKFREIFRKK"
     gene            24740..25375
                     /gene="trmB"
                     /locus_tag="ACGEUI_000929"
     CDS             24740..25375
                     /gene="trmB"
                     /locus_tag="ACGEUI_000929"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFF8165774.1"
                     /translation="MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIE
                     VGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALDKVLASEVPNVKLLRVDGSSLTN
                     YFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
                     YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFSNKGQVIYRVEANF"
     gene            25440..25526
                     /locus_tag="ACGEUI_000930"
     tRNA            25440..25526
                     /locus_tag="ACGEUI_000930"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25474..25476,aa:Ser,seq:gga)
     gene            complement(25573..26652)
                     /locus_tag="ACGEUI_000931"
     CDS             complement(25573..26652)
                     /locus_tag="ACGEUI_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8165775.1"
                     /translation="MAFYRKLGSGWEYRITYRDSQGKKREKSKRGFKTKTLAKVAAQQ
                     AEIDLNTLTADLLDITVLDYNRRWADIYKRPHITAKTWQTYTKNFKHIEHYFGTRKLK
                     SITHTFYQQVLNDFGAKVAQQTLDKFHYQIKGACKMAIRDGIIRDNFADGAIVRSQKP
                     AKEESEKFMEESEYLAFIKVAKSKVKYPSYLTTYIIAVTGLRFAEVQGLTWKDIDFEN
                     GYIDINKTFDYSISQNFGPTKNEQSIRKVPIDKNSLEILRNFKSDYYQDNKLGRVCFG
                     ASNNATNKVIKRVTGRNLTNHSLRHTYASYLIAQGVDLISVSKLLGHENLNITLKVYA
                     HQIESLKEKNDHQVKNIFQNLKFDG"
     gene            complement(26769..27320)
                     /locus_tag="ACGEUI_000932"
     CDS             complement(26769..27320)
                     /locus_tag="ACGEUI_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165776.1"
                     /translation="MDIAKLKEFAITAAGKAKDGAIKANELRKKASQESKIILPPALN
                     LELGAQTAIRKTIDGKYYIGFYSETPELYEFVSLQFDGSRIIEKTVTKGKTTQKGRSS
                     SVLGGAAIGKVLAPGVGTIIGGMAGGARKKKGTINTTATTTSEEIPGKATVALRNIQT
                     GEVKTLKTKLTQAQYVSVNNFFK"
     gene            complement(27331..27714)
                     /locus_tag="ACGEUI_000933"
     CDS             complement(27331..27714)
                     /locus_tag="ACGEUI_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFF8165777.1"
                     /translation="MMTPETVCQEKGIDLVYFDGRGTNTPGMFNKKHNVIAIDTYLDG
                     IYKHKVIYHELGHREHTASYYKLNKEKAELQADRCMIHHLLKEELSYWDNMEDFNYIQ
                     FMEKYELTSIADEVMIKEELEFLIS"
     gene            complement(27728..28129)
                     /locus_tag="ACGEUI_000934"
     CDS             complement(27728..28129)
                     /locus_tag="ACGEUI_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8165778.1"
                     /translation="MLFYRYFLSSHRKADKSMFSTFERIKELAKSRGISLGLLEEKLG
                     LSRNSIYTIKNKKPSAERLQLIADYFHVSTDYLLGRTDNPKIAQDGHASVAIDLKKDA
                     EETFFFDGHELNDEDIDLITSILETRIKNRK"
BASE COUNT         8392 a         4997 c         5982 g         8783 t
ORIGIN      
        1 gatttgatta tcaaatgatt ttaaagagaa ataatttctt agtcaaataa attatcagaa
       61 aagaggattg ttatgcagtt taaacacatc atctttgatg ttgtagatga tcttgcgaca
      121 ttgactttaa accgtccaga ggtttcaaac ggttttaata ttccaatttg tcaagagatt
      181 ctggtagcat tagcagaagt taaacgcgat acatctgtgc gttttttatt aattaaagcc
      241 gttggaaaag tgttttcagt tggtggtgac ctagttgaaa tgcaagaagc tgttgcaaaa
      301 gataatgtgc agtcgctagt caagattgct gaactggtac aagaaatttc ttttgcaata
      361 aagcatttgc caaaaccagt catcctttgc gccgatggag ctgtagctgg agcggctttt
      421 aatatagctt tagctgttga cttttgtatc gctagtactc aaacaaaatt tatccaagct
      481 tttgtaaatg ttggactagc accggatgcc ggtggtttat ttctattgac tcgtgcagta
      541 ggacttaatc gtgcaactca ccttgtgatg acaggtgaag gaattactgc tgataaaggt
      601 ctcgattatg ggtttgtcta ccgtacggca gaatctgata aactagacaa agtttgtctt
      661 caacttttaa agcgattgag acgtggctct tcaaattctt atgctggaat gaaatcattg
      721 gtctggcaaa gttttttcac aggatgggaa gattatgcta aggcagagct tgctattcaa
      781 gaggagctag ctttcaagga agattttaaa gaaggtgtca tagcttttgg agaaaggcgc
      841 cgaccaaatt ttcaaggtaa gtaagacagc tattgcttgc aaaatacttt gacctttgat
      901 ataatttgat tgtcaaagtt tattgctagg aggtgtttac ttggaatatg acaaaattaa
      961 ccaatactta gttgatattt tcaaccggat tttggttatt gaagaaatga gcttaaagac
     1021 gagtcagttt agtgatgtct ctttaaaaga gatgcacact attgaaatta tcggcaaata
     1081 tgatcaggtg actccaagtg atatcgccag agaattaatg gtaacgttag ggacagtaac
     1141 cactagctta aataaattag aagcaaaagg ttatatcgca agaacgcgat cacgctcaga
     1201 taggcgagtt gtttacctgt cattaacaaa aaggggtcga cttttggacc gcctccatgc
     1261 aaagttccat aaaaatatgg ttggacatgt cattgctgat atgagtgatg aagagatgca
     1321 ggcattggtg cgtggactag ggaatctaca tcaatttttg gaggatttgg tttaaatgat
     1381 tttttctaaa atcagtcagg tagcccacta tgttccacag cagttagtga ctaataatga
     1441 tttagctagc ataatggaca ctagtcatga gtggatcttt tctcgaacag gaatagcaga
     1501 acgccatatt tctagagatg aaatgactag tgacctagct attcaggttg ctgatcagct
     1561 tttaactcag tctgggttaa aagctgatgc tattgatttt attattgttg caactatcag
     1621 ccctgatgcc actatgccat caacagctgc gaaagttcaa gcagctattg cggctacaag
     1681 tgcttttgct tttgatatga ctgctgcttg tagtggcttt gtctttgcct tggctatggc
     1741 agataagctt attgcatcag gtgcctacca aaacggaatg gtaataggtg cagagacttt
     1801 atctaagtta gtaaactggc aggatcgagc gacagccgtt ctttttggag acggagctgg
     1861 tggcgtgcta ctggaagcca gtaaagataa gcatgtttta gcggaaacat tacacacaga
     1921 tggtgcgaga tgtcagagtt tgatttcagg agaaactagt ctttcatctc cttactcaat
     1981 tggcaaaaaa gccatagcga ctattcagat ggatggacgt gccatatttg attttgcgat
     2041 tagggatgtg tcaaaaagca tcctcacact aatggcgcaa tcagatatta caaaagatga
     2101 catagactat tgtttattgc atcaagctaa tcgtcgtata cttgataaaa tagcacgcaa
     2161 gattgatgtt cctcgggaaa aattccttga aaacatgatg cgttatggta atactagtgc
     2221 tgctagtatc ccaatcctct tgtcagaggc tgttcaaaag ggacagatta ggttagatgg
     2281 tacccaaaaa atcttacttt ccggttttgg tggaggtttg acatggggaa gtctaattgt
     2341 tagaatctag ttattcatgt gcctagcaca tttataaaaa caaatttata ttttttaagg
     2401 agaaataaac atggcagtat ttgaaaaagt acaagaaatt attgttgaag aacttggtaa
     2461 ggagacagaa gaagtaacac tagaaactac atttgatgat ttagatgcag attcacttga
     2521 tgttttccaa gttatttcag aaattgaaga tgcttttgat attcaaattg aaacagaaga
     2581 aggattaaat actgttggtg accttgttgc ttacgttgaa gaaaagtcaa aataaggata
     2641 aaagtgacaa gtaacatcag aagtctatga aaagcttact gctgttatgt tcatgttcat
     2701 cactaaaaaa aacaataagt attaatcaaa ccattagggt gggattagac tctttttagg
     2761 atctagctcg ctctttcttc ttactataat agtttgatta tcaaattatt ctccttattt
     2821 tttatagaaa gttattatga aaacacgtat tacagaatta cttaatattg attaccccat
     2881 ttttcaagga ggaatggctt gggttgctga tggtgattta gcaggtgcag tttctaatgc
     2941 tggtggttta ggcattatag gtggtggcaa tgctcccaaa gaagtcgtta aagctaatat
     3001 tgatcgtgtc aaagctatta ctgatagacc ttttggggtt aatatcatgc ttttatctcc
     3061 ttttgctgat gatatcgttg atctggtcat tgaagaaggt gttaaagtag taacaacagg
     3121 cgcaggaaat ccaggaaagt atatggaaag actgcaccag gcgggtataa tcgttgttcc
     3181 tgttgtccca agcgttgcgc tagccaaacg tatggaaaag cttggggtag atgctgttat
     3241 tgctgagggt atggaagctg gaggacatat tggcaagtta acgactatgt ctttagtaag
     3301 acaagttgtt gaagcggttt cgattcctgt cattgcggca ggtggtatag ctgatggtca
     3361 tggtgcagca gcagcattta tgttaggagc agaggctgtt caaattggaa ctcgctttgt
     3421 tgttgctaaa gaatccaatg ctcaccaaaa ttttaaagat aaaatcttag cagcaaaaga
     3481 tattgatacg gtgatttctg cgcaggttgt gggccaccct gtccgttcta ttaaaaataa
     3541 attgacctca gcttacgcta aagcagaaaa agcattttta attggtcaaa aaacagctac
     3601 tgatattgaa gaaatgggag caggatcgct tcgacacgct gttattgaag gcgatgtagt
     3661 caatggatct gttatggctg gccaaattgc agggcttgtg agaaaagaag aaagctgtga
     3721 aacgatttta aaagatattt attatggtgc agctcgtgtt attcaaaatg aagctaagcg
     3781 ctggcaatct gtttcaatag aaaagtagat aaacacatga aggataatga taatgacaaa
     3841 gacagccttt ttatttgccg gtcaaggtgc tcaaaaatta gggatggcaa gggattttta
     3901 tgataacttt gctattgtaa gaaaaacctt tgatcaagct agtcaagtat tgggatacga
     3961 tttgcgccgt ttgattgata gtgacgagtt aaaacttaat caaactagct atacgcaacc
     4021 agctattttg acatcatcaa ttgctattta ccgtgttttg ggcttacatc acgttaaacc
     4081 ggatatggta gctggtcttt ccttaggaga atactcagct ttggtagcat caggggcact
     4141 ctcttttgaa gataccctat ccttagtagc taagagaggc cgcttgatgg aggaagcagc
     4201 accacaagga tctggaaaga tggttgccgt tatgaataca gacgtgcaag tcatcgaaga
     4261 agtctgtcaa atagctgcta aacatggagt ggttgctcca gcaaactata atactcctag
     4321 tcaaattgta attggtggtc agacagatgc tgtgaacgtg gcagttgaac ttttaaagga
     4381 aaggggagtt aagcgtttaa tccctttaaa cgtgtcaggt cctttccaca ctgctttgtt
     4441 agaaccagct agccgcttgt tggctaaaga gttggaaaga tacaacttca gtgacttcaa
     4501 gattcctttg gttggtaata ccgaagctaa tattatggaa aaagaccgta tcccagaact
     4561 attagcccgt caagtcatgg agcctgttcg tttttatgac agtgttgcga ctttagtaga
     4621 aagtggcata acacaattca ttgaggtagg accaggtaag gttttgacag gttttgtgaa
     4681 gaaaattgat aaaaatttac tatgtactag tgttgaaaac atggtaagct tacgtttatt
     4741 tttagataga gaggacattc atggaaatta aaggaaaaaa tatatttatc acaggctcaa
     4801 cgagaggtat cggtttagca atggctcatc aattcgccag tttaggagct aatattgtct
     4861 taaatggtcg ctctgctatc tctgaggagc ttgttgctag ttttactgac tacggcgtta
     4921 cagttgttac catctctggg gatgtttcag aagcatcaga agctaaacgt atggtcaatg
     4981 aagctatcga aagtttaggt tcgattgatg ttttagttaa caatgctggc attactaatg
     5041 ataaattgat gcttaaaatg acggaagagg attttgagcg cgtcttgaaa atcaatttga
     5101 ccggtgcttt taacatgact caatcagtgt taaaaccaat gatcaaggcc cgccaaggag
     5161 caattattaa tgtctctagt gtggttggtt taacaggcaa tattgggcaa gcaaactatg
     5221 ctgcttctaa ggcaggaatg attggtttta ccaagtctgt tgcccgtgaa gttgctgctc
     5281 gtaacatttg cgtgaatgct attgctccag gatttatcga gtccgacatg acaggtgtcc
     5341 ttcctgaaaa aatgcaagag caaatcttaa gtcaaatccc aatgaaacgt attggaaaag
     5401 ctcaagaagt tgctcactta gcaagcttct tggtggaaca agattatatt acaggacaag
     5461 ttatcgctat tgatggcggt atgacaatgc aataaggaga cgtagtgaaa tgacatttaa
     5521 aagagtagtg gtaacaggtt atggtctaac atctccaatc gggcatgatc ctgaaacgtt
     5581 ttggaacaat ctgaaagctg gccaaattgg tattggtcct attactaaat ttgacacgac
     5641 tgattatgca gtcaagaatg ctgctgaaat tcaggatttt ccatttgaca aatactttgt
     5701 gaaaaaagat ttgaaccgtt tcgatagata ttctctttat gctctttacg cagcgaaaga
     5761 ggctattaat catgctgatt taaatattga gatggttgat tccgatcgat ttggcgttat
     5821 tgttgcttca ggtattggtg gaattgcgga gattgaagag caagtgattc gcttacatga
     5881 aaaagggcca aaacgtgtga agccaatgac gttgccaaaa gcgctaccaa atatggctgc
     5941 aggaaatgtc gccatgtctt taaaagctca aggagtatgt aaatcaatta atactgcctg
     6001 cgcttcttca aatgatgcga ttggggatgc ctttcgtgcc attaaatttg ggactcaaga
     6061 tgtgatgata gttggtgggt cagaggcggc cataaccaag tttgccattg ctggcttcca
     6121 aagtttaaca gctctctcaa caactgaaga tccaagcaga agctctattc catttgataa
     6181 agaccgcaat ggttttatca tgggagaagg atcaggaatg cttgttttag aaagcctaga
     6241 acatgcgcaa gaacgcgggg cgacaatctt agcagaaatt gtgggttatg gaaatacctg
     6301 cgatgcctac catatgacat ctccaaatcc tgagggatta ggagctcgta aagccatcca
     6361 cttagcctta caagaagctg gtattgaagc cagtgctatt aattatgtca atgcgcacgg
     6421 aacttcaacc ccagctaatg aaaaaggaga gagtcaagca attgtagccg ttcttggtaa
     6481 ggatgttccg gtgtcttcaa caaaatcgtt tacgggtcac cttcttggag cagcaggcgc
     6541 tattgaagct attgccacta tcgaggctat gcgtcataac tatgttccga tgacggctgg
     6601 gacacaagct ttatcagagg atatcgaagc aaatgtcatc tttggagaag gaaaagaaac
     6661 agctatcaac tatgccattt caaatacttt tggatttggt ggtcataatg ctgtattagc
     6721 tttcaaatgt tgggaggagt aggttgaata ttcaagaaat caaagatttg atggctcagt
     6781 ttgatacctc aagcctacgt gaatttttat tcaaaacaaa tgaaggtgag ctaatcttta
     6841 gtaaaaacga acaacacctc aatgcatcga catcaaatca agaacatgct gtgcctgtac
     6901 cacaggtaca acttgttccg aatccaactg cttcggaagc gtccagtcca gcatcagtta
     6961 aggatgttcc tgtagaggag caaccacaag cagaatcttt tgtagcagaa ggtgatattg
     7021 ttgaaagtcc tttagtcggt gtagcctatc ttgcagctag ccctgacaag ccaccatttg
     7081 tggcagtcgg agatactgta aaaaaaggac aaacccttgt gattatcgaa gccatgaagg
     7141 tgatgaacga agtgccagcg ccatgtgatg gtgtgattac agaaattttg gtatcaaatg
     7201 aagatgtcat tgaatttgga caaggattgg tgcggatcaa atgatggata ttagagaaat
     7261 tcaagcggct ttgcctcatc gctacccaat gttattggtt gatcgtgtgt tagaagtgtc
     7321 agacgaccat atcgttgcca ttaaaaatgt gactattaat gagcctttct ttaacgggca
     7381 ttttcctcac tatcctgtga tgccaggtgt tctgattatg gaagctttgg ctcaaacagc
     7441 tggtgtctta gaattgtcaa aagaagaaaa taaaggcaag ttggtctttt acgctggaat
     7501 ggataaagtg aagttcaaaa aacaagtcgt ccctggtgat caacttgtga tgacagcaac
     7561 ctttatcaag cgtcgcggta ccattgctgt cgttgaagcc agggcagaag ttgatggcaa
     7621 gctagcagca agtggaacat taacctttgc ctgtgggcaa tgagaatgat tgttctgcgg
     7681 aggaagaaac ccttatgttt aaaaaaatct taattgctaa tcgtggtgaa attgcagtgc
     7741 gtatcattcg tgctgcacga gaattaggga tttccacagt tgcagtttat tcggaagcgg
     7801 ataaggaagc tctgcacacg atcttggcgg acgaggctat ctgtattggt cctgcccgtt
     7861 ccaaggaatc ctatcttaat atgaattccg tcttgtcagc agctattgtg acaggagccc
     7921 aagcgattca cccagggttt ggctttttaa gtgaaaactc aaagtttgcg accatgtgtg
     7981 aagagatgaa tattaaattt atcggtccat ccgcatcggt tatggataaa atgggtgata
     8041 aaatcaatgc tcgttctgaa atgattaagg caggagtgcc cgttattcca gggtcagatg
     8101 gtgaagttta taacgctcaa gaagccttag ctattgccaa taagattggt tatcctgtca
     8161 tgctaaaagc atcggcaggt ggtggaggta aagggattcg taaagtggag acagaagcag
     8221 atttggaggc ggctttcaat gctgccagtc aagaagcttt gggagctttc ggaaatggcg
     8281 ccatgtattt ggaaaaagta atttatccag ctagacatat tgaggtgcaa attttaggag
     8341 acgcttacgg taacatcatt cacttgggag aacgggattg ctccttgcaa cggaataatc
     8401 aaaaagtctt ggaagaaagt ccatccattg ctattggcaa taccctcaga cacgaaatgg
     8461 ggcaagcagc ggttagagca gctgaagctg tagcctatga aaatgcggga accattgagt
     8521 ttttgttgga cgaagacagc gagaaattct atttcatgga aatgaatacc cgtatccaag
     8581 ttgagcatcc tgtcaccgaa tttgtgacag gagtggatat tgtcaaagag caaatcaaaa
     8641 ttgcagcagg tcagcctttg gcgattaatc aagaagacat cactatcacg ggacatgcta
     8701 ttgaatgtcg tatcaatgct gaaaatactg cttttaactt tgcaccgagt ccaggaaaga
     8761 ttacagactt gtatatgcca agtggtggtg taggactacg agttgattca gcagtttaca
     8821 atggctatgc cattcctcct tattatgaca gcatgattgc taaaatcatc gttcatggaa
     8881 gcaatcgatt tgacgctttg atgaaaatgc aacgtgcctt agttgaattg gaaattgaag
     8941 gcattatcac caatacagac ttccagctcg atttgatttc agataagcga gtgattgcag
     9001 gagattatga tacctctttc ttgatggaaa cgttcctgcc acattacaat aaagattagg
     9061 agaaaatatg gctttatttc gtaaaaaaga taaatacatc cgtattactc ccaataattc
     9121 cctcaaagga tctgtcagcc ataatgtccc agaagttcca gatgaactct ttgcgaaatg
     9181 cccagcttgt aagcacatga tttataagaa agatttggga ttggcaaaga tttgcccaac
     9241 ctgttcttac aattttagga tttctgccca agagcggctg accttaacag ttgatgaagg
     9301 ctctttccaa gaattattta caagtattga aaccaaggat ccgttacgtt ttccaggtta
     9361 tcaggaaaaa ttacaaaaag ccaaggaaac gacaggtctg catgaagctg ttttgacagg
     9421 gaaagctatg gttaaggagc aaaagattgc tcttgctatt atggattctc actttattat
     9481 ggctagtatg ggaactgttg ttggggaaaa aattacccga ttgtttgaat tggctatcga
     9541 agaaaacctg ccggtggtga tttttacggc ttcaggaggc gcccgtatgc aagagggtat
     9601 catgagcctc atgcaaatgg ccaaggtctc tgcagctgtg aaaagacatt ctaatgcagg
     9661 cctcttttac ctcactattt tgacagatcc aactactggt ggggtaacag ctagttttgc
     9721 catggaaggg gatattattt tggcagaacc tcaatccttg gttggctttg caggtcgtcg
     9781 tgtcattgag accacagttc gtgaaaactt gccagatgat ttccaaaaag cagaattttt
     9841 acaagaccat ggttttgtgg atgctattgt gaaacgaaca gagttgcgag acaagattgc
     9901 ccacttagta gcatttcatg gaggtggcca atgacagatg tatcaagaat tttaaaagaa
     9961 gcgcgtgatc aagggcgttt aacaactttg gattacgcca accttatttt cgatgacttt
    10021 atggaactgc atggcgatcg ccatttttca gatgatggtg ccattgtagg tggcctagct
    10081 tatttggcgg gacaacctgt tacggtcatt ggtattcaaa aaggtaagaa tttacaggat
    10141 aatttggcaa ggaattttgg ccagcccaat ccagaaggtt atcgtaaagc tttgcgcctt
    10201 atgaaacagg cagaaaaatt tggacgacca gttgttacgt ttatcaatac tgcaggagcc
    10261 tatccaggtg tcggtgcgga agaacgagga cagggtgagg ccattgctaa aaatttgatg
    10321 gaaatgagtg atctcaaggt tcccattatc gccatcatta ttggtgaagg aggctctggt
    10381 ggtgcattag ccttagcggt tgccgatcag gtctggatgc ttgaaaatac tatgtatgcg
    10441 gttcttagcc cagaaggctt tgcttctatt ttatggaagg atggttcaag ggcgaccgag
    10501 gccgctgaat tgatgaaaat cacagcgggt gaactctaca aaatgggaat agtagaccgt
    10561 attattccag aacatggtta tttttcaagt gaaatcgttg acatcatcaa agctaacctc
    10621 atcgaacaaa taaccagttt gcaagctaag ccattagacc aattattaga tgagcgctac
    10681 caacgctttc gtaaatatta acaacatcct tttggagtct ccctacgtga agggaggcct
    10741 ttccaaaagg gtttttaact agtcttgtaa ataaggaaat gggtatacta ataagacttt
    10801 tgataaacta gtgtgaagaa aagtgttgtt aaaggggcaa tgtagtgagt ttcaagtgat
    10861 gtggtggctc tttaaaatag taaaccccag aaatcacttt aatgatctct ggggtgaatt
    10921 tattatttag gtgaaatgac ggtttcgcca cccatgtaag ggcggagtac ttcagggatg
    10981 gtaacagaac catcttcatt ttggtagttt tctaaaatgg ctgctacggt acgaccgaca
    11041 gcaagtcctg acccattaag ggtgtggaga agtttgacct taccgtcagc ctcatcacgg
    11101 taacggattt gagcacgacg tgcttggaaa tcttctgtat ttgaacatga tgaaatttca
    11161 cgataggtat tttgagctgg aatccagact tcaaggtcat aggttttagc agctgagaag
    11221 cccatgtctc cagtacaaag ggaaatgacg cgatatggca aaccaagttt ttgaagaata
    11281 ttttcagcat tagcagtcat tttttctagt tcttggtaag attcttctgg tttagcaaat
    11341 ttaaccattt caactttgtg gaattggtgt aggcggataa ggccacgtgt gtcacgaccc
    11401 gcagaacccg cttcagaacg aaaagatggg ctcattgcag taaagtaaat tggcaattct
    11461 ttgccgtcta ggatttcccc acggtaataa ttggtcaaag gcacttcagc agttggaatc
    11521 aggacaaagt tggtatcagc taattcaaag gtgtcctctt tgaattttgg atattgtcct
    11581 gtaccaaaca tggaatcgtg gttaaccatg taaggtgtga tgatttcttg ataaccttct
    11641 ttgatatgtt catccagcat gaagttataa agagcacgct ccaaacgagc accaagattt
    11701 ttatagaata agaaacgagc accagttacc tttgccccac gttcccagtc taaaatatca
    11761 agatcttcgc ctagatccca atgagcctta acttcgaagt caaagtcacg aggcgttccc
    11821 caacggcgaa tttcgacatt gtcttcttcg tcagctccaa cagggacaga atcgtgagga
    11881 gtattaggta aaacagtgat gatgtcagta acttgctggt cgatagctac tagttgattg
    11941 tcaatagttt tgatatctgc agagactttt tgcatatcgg caatttgttg agtggcatct
    12001 tctttttgac gttttgcttg ggcgatggct gcagaagcaa tattacgctc tgcttttaat
    12061 tcttctgatt gcactaatag agcacgacgt ttttcatcaa gttctttaag atgggtcagg
    12121 gtgtcttcag aaacaccgcg gttttttaat ttggcagcga cagtgtcaaa atcggtacgg
    12181 atacgtttaa gatctagcat acagaagctc ctttaagata gttattagta gctgtcagtg
    12241 aaacaaggct aagtaagaca gttgggtctt gtgattctct gacgatagaa aaagcacagc
    12301 ttatgaagat gctggagcgc tttggcgcgg ttccatccag cttcacaatc tgtgcactta
    12361 atctctattt tattgatgac acggtagaat ttcatatcat tttccctatc tgttcacagc
    12421 atccacagac tctctgaatg aaaaaaaggt attacttatc cgttaggact attataacaa
    12481 aaaacaagaa aaagtaaact caaatgaggg aatcttttcc actaaatagc aagttagagc
    12541 tactattctt tgctttcttg aaatgataaa tcggcactgc tcattatttt tttaatgtcg
    12601 aaaaaatttg ttaataaagg tttgcatcaa ggttggtaat gtaatcactt tctcattacc
    12661 aatatgttga cgagtgattt ttaagatttt tccagagtct ccagaagcaa ataaaaactt
    12721 gccttggtcc gtaaaaattt caaagtggcg gctaactttt tttcttgaaa cattagcccc
    12781 gatgtgattg atagctgtcc aaggaatttg aatataatca ttcacatttt tatcgttgta
    12841 aaattcaagg gccttgtcac cgagtaaaat atgaccaact ttgcctccca ttccaaggta
    12901 ggagacagct tttgtcttgt attcgacact agtgtttaaa gattgagcca tttaagttcc
    12961 tttcattgcg tagttgtagt tattatacca tattttaagt gggattttcc tgacataaag
    13021 cataaaaaag aagcctgagt tcatctcaga cttccattgg ttgattacat aatacctgca
    13081 aggtgagcaa ggataccaaa tgcaaacaag ccgatgatga ttgcgattgg agaaactttt
    13141 ttcttcaata accacatgca aaggaatgta aggagtaatc cagctaaacc tgggataaga
    13201 gagtccaatt gtccttgaag ggtttgagct tggactttat caaggctcat accaccaata
    13261 gcgttggcaa ggatagtttt caattcagca cctttaacag cgccatctgg gaagaccaca
    13321 taggcaccat ctgacaattg ttttccagga aggtcgatag taaagttgat agatacccaa
    13381 cgttgaacaa gcactgcaag gataaacatt ccaaggattg aagcaccttt agtaatgtct
    13441 tggaggatac cgcctgacat gtctttagta atttcagaac cagctttgta accaaattct
    13501 tgagtatacc ataagaaggc catacggata aggttccatc caaagaagaa cagaagtgga
    13561 ccaacgatgt tacctgttga agccaaagaa gcaccaagtg ccccaaggat aggacgaatc
    13621 gtaaaccaga atactgggtc accgatacca gcaagaggtc ccatcatacc gattttaacc
    13681 ccttggatag ccttgtcatc aattggcgta ccatttgcgc gttcttcttc aagggcaagt
    13741 gttacaccaa tgattggagc tgcaacgtat gggtgagtgt taaagaattc catgtggcgc
    13801 tcaagagcag ctgcacggtc ttcaggagac gtatacaatt ttttaagagc agggataagt
    13861 gcataagccc agcccatgtt ttgcatacgt tcgtagttcc aagaaccttg taagaattgt
    13921 gaacgccacc aaacacgttg acggtctgat ttagttaatt taatttgttc agtcatttgt
    13981 tcttacccct ttctagtagt cttctaagat gtcgccgatt ggatcagctg aacctgctgc
    14041 attaccaccg ttaccacctt ttttagaaag attaaggtag atgaaggcaa ttgctacacc
    14101 gataacaccc atagcgataa gggttaattg gctgatagct gcaagagcaa aacctagggc
    14161 gaagaatggc catacttcac gagtagccat catgttgata accatagcat aacctacagc
    14221 aacaaccatc gcaccaccga tttgcatacc ttcgttcaac cagtttggca tagcattcaa
    14281 aacagattgt acggctgaag taggaacagc aacaagaaga gctgcaggga cagcgatacg
    14341 caatccttga agaagaaggg caataaagtg gaagcgttca atacctgcga aattaccttc
    14401 agcggcagct ttgtcaccag cgtgtacaag ggcagttgac aaagtacgaa cgatcattgt
    14461 aaggaaaaga ccagcaacag caagtgggat agctgttgag taagcaaagg taattccttt
    14521 ttgagtaaag tcgccacttt tgaccatgat gatagcagct gcaacagagg caagggcagc
    14581 atcaggggca acggcagcac cgatgtttgc ccaaccaaga gcaagcattt gaagtgtacc
    14641 accaaggatg acaccagctt caaggtgtcc agttacaagt ccaatcaaag tacaggcaac
    14701 aagtggttgg tgcatttgaa attggtcgag gataccttca agaccagcga agaaggcaat
    14761 gataacgacc aaaattgcag aaataatcga aatatctgac atgttgtaat cctttcaaag
    14821 tatagtaaac gaggactttt tgctaataag ttagctagtc atcgttttaa aaccttatag
    14881 cgtttgaaac ttatttaatg ttagcttttt ggataagctc aaataagttt ttcttagaat
    14941 cgttaggaac tttacggacg tcgaatgtaa cacctaggtc gcgtaatttt tcaaaagtag
    15001 caacgtcttc tttgtccatt gataaaacgt tgttaaccat tgttttacca gttgagtgag
    15061 ccattgaacc aacgttcaat tcgttgattt caacaccacc ttcaaccgct cgaagggcat
    15121 cttgaggggt ttggaaaaga ataagtgcgt gtgtgttacc aaaacgagga tctttagaag
    15181 cttcaatcaa tttgctaatt ggaacaacgt tagctttgac accaccaggt gcagcctgtt
    15241 tgatcaattg tttgcgaagg tcatcttgag caacctcatc agaagcaaca atgatacggt
    15301 cagccttaga agctggagtc catgctgttg caacttgacc gtgtaaaaga cgagtgtcaa
    15361 cacgagcaag attgatcttt aatttaccat ctccgatgac agttccagca ggaatagctc
    15421 cttgaagtgc gttaacaact ttttcagttg cagcagtttc ttcaactggg ttaaggtctt
    15481 caggaagggc tttgataccg tccttagatt ccttaatgat gtttgcagca acttgttcaa
    15541 cacctgcacc agcatccata agacgttcag tgtaagcttg aataagcata ggcaagttga
    15601 gaccagtgat gatcgccatt ttgcggtcag gattttcacc tgccacacga ctagcttggt
    15661 taaatggaga accactccaa aggtcagcta gcacaaggat ttcatcatca gcatcaaatt
    15721 gttggatagc attgttgaag tgtccataca aatcatctgg tccttcgttt ggcatgaaag
    15781 taacaacttg aactttctct tgttcaccaa aaatcataga accagattga tgaatacctt
    15841 cagcaaattt accatggctg gcaataataa taccgatacc cattcttgtt cttcctcctt
    15901 taaaatattt ttcgttgaaa tgaagaagaa taatctcctt ccaaaactaa attttggcaa
    15961 ttctttttca cttaaacgaa attcaagaac gtttctattt taaaacgttt tcactaaaaa
    16021 atcaagccct ttcggaaaaa tagtacaaaa catgaaaaaa ttttcagaat ttaggattaa
    16081 ataggcagat tattgatatt aaaggtgttt tcaagctgtt taaaacaaaa atatgctgaa
    16141 aaatgttgaa attattaaaa tgattttcat tttttgatag ctgttgacag aagaagaacc
    16201 accattttct gaaaaatagt ggtttcgttt gctactacaa gaaaagttct tccaattttt
    16261 tggcaacacc gtcttcttcg ttggaaaaat taagctgctg atcagcataa ggcagtagca
    16321 cagggctagc atttttcata gcataaccag ttccagcaaa agctagcatc tcagtgtcat
    16381 tgtgttcatc accaaaggcg attaaatcct ttttatccat attgtaaatt ccaaggagat
    16441 agttcagagc gtaagcttta ttgacattct tagacgaaat ttcaagaatg ttgagagggc
    16501 ctccccaaga atcaatttca atttcatctt tgaagcaagc acgcatgttt ttagctaagg
    16561 catacttgtc ttcgtgatgc gtttgcatca ataaggcgtt tggatttcga gtgattttgg
    16621 taatctctaa tgccatgtcc tgagtaattt catcaacacc aaagagttgc gggtcgatac
    16681 tttctggatt ggtcatggtg atgtagacat tttttcgata ctcactggca ataaaatcca
    16741 tctggaaatc atcctgatat tttagtaacc ttagtaggta gcctttgtcc aaagtgacat
    16801 tatgttcata ggcccatttt tgttcaggca tgtgggtcaa ggccccatta aaagtgatga
    16861 ttggggtctt taaattgagc tgcagatagt agcccagtgc catgcggtaa ggacggcctg
    16921 ttgaaataat gacgtggtgg cccttgtctt ggacagcttt gatggttttt tgagtatagg
    16981 tagagatggt gttgtcatga tgtaataagg ttccatccaa atcaatggca atcaattttt
    17041 tagtcataat taaattatag caaattttgc tccaagataa agagaaaaga taagttaagc
    17101 gtattcatta gtaaaaaaga taggataact gtttttttaa tgaataaatg aagttataga
    17161 cgttgaaaag tgaacaaaaa aatggtaaca tattgttatt gttcatttta atacgaacaa
    17221 taacaataat aatgaggtgt caaaaagact tatctagtga taagcgcttt ttgattttac
    17281 ctactattta attaaggaga ctccatttca atggaaaagt tttttaagtt aagcgaaaat
    17341 gggacaactg tctcaactga gattatggct ggtttaacga ccttttttgc catgtcctat
    17401 attttgtttg ttaacccaag tattttaggt gcagcgggga tgccctctaa tgctgtcttt
    17461 ttggctacga ttatcgcagc agccatatca accttaatta tgggactatt tgccaatgtg
    17521 ccttatgcgt tggcaccagg aatgggactt aacgcttttt tcacttatac agttgttttt
    17581 gctttagggt tttcatggca agaagccttg gcaatggttt tcatttgtgg attattcaat
    17641 atttttatta ccgtaaccaa gtttcgtaaa agtatcatca aggcgattcc agttagttta
    17701 cagcatgcta ttggtggggg aattggtgtc tttgtagctt atttaggatt taaaaacgca
    17761 aatatcatta ctttttctat ctctgctgaa aatatagtaa tggtaaatgg tgttgaaccg
    17821 gctaaagcat cggctaaaac atttgcagat ggtctattat ttgtagacgc caatggtgga
    17881 gttgtaccta cgatttctag ttttacggat tccggtgtat tacttgctat ttttggttta
    17941 cttttaacga cagctcttgt gattcgaaat tttagaggtg ctattttaat tggtattgtc
    18001 gcaacaactc ttgtaggtat tcctttagga atagtggatg tgtccaacct caattttggg
    18061 atcagccata ttggtgaagc ttggactgaa ttaggtacaa ctttccttgc agctttcgat
    18121 ggtttgagtt ctctttttag cgattcaagt cgtttaccgc tagttttcat gactattttt
    18181 gcttttagtc tatcagatac ttttgacaca attggtacct ttatcggaac tggtcgtcga
    18241 acaggtattt tctctcaaga cgatgagaat gctttggaaa atagtatagg ctttagttca
    18301 aaaatggacc gtgcgctttt tgcagatgct atcggtactt ctattggggc tttggttgga
    18361 acttcaaata cgactaccta tgttgaatca gcagcaggaa ttgctgaagg tggacgtact
    18421 ggactaacag cagtctccac cgcagtatgc ttcttattat caatattgct attaccgctt
    18481 gtaggtattg tcccagctgc tgctacggct ccagctttaa ttattgtggg tgtcatgatg
    18541 gtgtcttctt ttcttgatgt taattggagt aaatttgcag atgctcttcc agcttttttt
    18601 gcagctttct ttatggcgct gtgttactct atttcctatg gtattgccgc tgcctttatt
    18661 ttctattgtc tagtaaaagt tgttgaggga aaaacaaaag atattcaccc tattatttgg
    18721 ggagcaacct tcttgttcat tgtaaatttc atcatattaa ctatcttata accaaataat
    18781 ttacttattt tgctactaac tgccgcttct ttaattgatt ttaaccttta atataaagaa
    18841 gcgtttttac tttacgaata ccttattgga gtcatcttct aggtagtgct tggaagtatg
    18901 ttttttttct gatataatgt tgctatgttt tatagtgaaa atgaatatac tcttaaagct
    18961 tacggagaaa ccctaggtac ttatttgtct attggagatg tgattgtatt gtctggcgac
    19021 ttaggtgccg gtaaaacaac attggcaaaa ggtattgcta aagggatggg gattagtcaa
    19081 atgatcaaaa gcccaactta tactattgtt agagaatatg aaggacgtct acctctttat
    19141 cacttagata tttaccgtgt tggtgatgat ccagactcta ttgatttaga tgattttctt
    19201 tttggtaatg gtgttaccgt tattgagtgg ggagaattat taggagaggg attattacaa
    19261 gactatttgc aaattaccat tactaaacga gacaaaggtc gtcaactaga cttgttagcg
    19321 catggtgagc gctctcgtca gcttctggag ataatgtcac atggttcagc aaagtctcgt
    19381 tattgaagaa ggcgtacctc aagatgcaaa agctgtactt tccttagtga cggcagctgc
    19441 caaagaaaca gattttataa caggcgtaga gactatatta gaggctactc ctcaagaatt
    19501 gtcagacttt ttatcaagaa gtcaaacctc ttttattgat ttttgcctgc tagctagatt
    19561 agatgaaaaa gtagtagggc ttttaaatct ttctggggaa gtattatccc aaggccaagc
    19621 tgaagcagat gtatttatgt tagttgctaa gacttaccgt ggttatggta tcggtcaatt
    19681 attgttagag attgctcttg attgggctga agaaaaccct tatatcgaaa gccttaagtt
    19741 ggacgttcaa gtaagaaata ctaaggctat ttacctgtat aaaaagtatg gttttcgtat
    19801 tgaaagtatg cgaaaaaacg acatcaaatc aaaaaatgga gatgacttag atgtttatca
    19861 tatgcgtaaa tctgtaagtc agtaagtgaa tcgatgaaaa ttggaaaaaa aatagtttta
    19921 atgttcacag ctattgtgtt aacaactgtc ttggcattag gtgtctatct aactagtgct
    19981 tataccttct caacaggaga attatcaaag acctttaaag atttttcgac atcttcaaac
    20041 aaaagtgatg ccattaaaca aacaagagct ttttctatct tgttgatggg tgttgataca
    20101 ggctcttcag agcgtgcctc caagtgggaa ggaaacagtg attcgatgat tttggttacg
    20161 gttaatccaa agaccaagaa aacaactatg actagtttag aacgagatac cttaaccacg
    20221 ttatctggac ccaaaaataa tgaaatgaat ggtgttgaag ctaagcttaa cgctgcttat
    20281 gcagcaggtg gcgctcagat ggctattatg accgtgcaag atcttttgaa tatcaccatt
    20341 gataactatg ttcaaattaa tatgcaaggc cttattgatc ttgtgaatgc agttggaggg
    20401 attacagtta caaatgagtt tgattttcct atctcgattg ctgaaaacga acctgaatat
    20461 caagctactg ttgcgcctgg aacacacaaa attaacggtg aacaagcttt ggtttatgct
    20521 cgtatgcgtt atgatgatcc tgagggagat tatggtcgac aaaagcgtca acgtgaagtc
    20581 attcaaaagg tattgaaaaa aatccttgct cttgatagca ttagctctta tcggaagatt
    20641 ttatctgctg taagtagtaa tatgcaaacg aatatcgaaa tctcttctcg cactatccct
    20701 agtctattag gttatcgtga cgcacttaga actattaaga cttatcaact aaaaggagaa
    20761 gatgccactt tatcagatgg tggatcatac caaattgtta cctctaatca tttgttagaa
    20821 atccaaaatc gtatccgaac agaattagga cttcataagg ttaatcaatt aaaaacaaat
    20881 gctactgttt atgaaaattt gtatgggtca actaagtctc agacagtaaa caacaactat
    20941 gactcttcag gccaggctcc atcttattct gatagtcata gctcttacgc taattattca
    21001 agtggagtag ataccggcca gagtgctagt acagaccagg actctactgc ttcaagccat
    21061 aggccagcta cgccgtcttc ttcatcagat gctttagcag ctgatgagtc tagctcatca
    21121 gggtctggat cattagttcc tcctgctaat atcaaccctc agacctaacg ttctgagggt
    21181 ttttatgttt aaaatatccg attttgaata taaaaaagac aggtatcaaa gatgatagct
    21241 gtctgtgatt tcaaataaag tatatgaggg agataatact atctggaatg gttgttagtt
    21301 aaaatgacgg tttggcaagt aggtgattca ctagaaatta gctcactatc ttcttgatat
    21361 ttggccatac gattttggat ttcagtgagg tgtgcttgtg tctcttggtt aaagtagcgc
    21421 catttatggg ccaagtcctg actaatattt tgaattactt ttccttgact gtgaatgagg
    21481 tcaaggtttg ctttaatgtt atctaaatca agttgggcag catcttttgc ttgtctactg
    21541 atggataaca gctcttttat ggtacaacgt ttttgatatg ctttgtaagc ggtaaaagat
    21601 aataatccga cggctatcac ggttttaact ttcatgaact aacctcctga ttgatttgtc
    21661 gggcgagctt ttccaagaca ggaaagtcag gctcatgagt ggtgtattga attgagatac
    21721 cgtcttcttc attataacga ggaacgagat gaacatgagc atggaaaaca gtttgtcccg
    21781 ctaaggcttc gttattgtta atgatattca tggcagtagc gccagtagct gactggatag
    21841 cacgagcaat tttaggaata cgagcaaaga gatgactagc tgtctcagca gtcatctcta
    21901 gtaagttacg aacatgttgc ttaggaatga ctagagtatg cccttttgta gtttgtgaga
    21961 tatctaaaaa ggcgaggacc tgctcatctt cgtagacttt cgaagagggg atatcccctt
    22021 gaatgatgct gcaaaaaata caattttcca ttttatatca ccttctctac ttgaatttgt
    22081 tataatatat tataagtata tcaaaaacta gaaagaaatt atatgttaaa cattaaaaat
    22141 ctaacaggag gatatcacaa tatccctgtt ttaaacgatg tctcattttc tgtagacaat
    22201 ggagagttag tcggtttgat cgggctaaat ggtgctggaa agtcaacaac tatcaacgaa
    22261 attattggct ttttgaagcc ttatcaagga agtatttcta ttgatggttt aactctagca
    22321 gaaaatgcag ttgcctaccg tcaaaaaata ggttttatcc cagaaactcc tagtctctat
    22381 gaagaattga ccttatcaga gcatatcaac accgtagcta tggcttatga tatcgactta
    22441 gaagtggcac aaaaacgagc acaacctttc ttggaaatgt ttcgtttaac tgacaaactt
    22501 gaatggtttc ctgtcaattt ttctaaaggg atgaaacaaa aagtcatgat tatttgtgcc
    22561 tttgtcattg atccaagtct atttattctt gatgaaccat tcttagggtt agacccttta
    22621 gctatttcag acttaatcca aacgctagaa gtcgaaaaag caaaaggaaa atctattttg
    22681 atgagtacac atgttttgga ctctgctgaa agaatgtgtg atcgttttgt gattttgcat
    22741 catggacaag ttcgtgccca aggaactctt gctgacttgc aagaagcttt tggtgaccgt
    22801 tcagcgagct taaatgatat ctatcttgca ttaacaaaag aggattaagt atgaaagcat
    22861 tatttctgaa acgccgtcaa gactttcaaa aacaacaaaa caaatacctt cgttacgtct
    22921 taaatgatca ctttgttttg gtgttgatgt ttttattagg ctttgctatg gtgcagtatg
    22981 gtcagctact aaaccatttt ccaacgaatc atctacctat tcaggtttgc cttgggatat
    23041 tgatcccatt attgctcagt atgggatcta tagcaacgta tctagaagag gcagatcagc
    23101 atttcttact tccaaaagaa gaagaagtga tttcttatat caaacaagct gagcgactct
    23161 cttttttgct ttggggaact ttgcagactg ctgtattgct ctttttatat cctattttta
    23221 gacgtttagg tctgtcttta tttattttta ttatcttagt tttgatatta ttggcactaa
    23281 aaagagtagt gcttagtcga aagacaagat attttttgag aggaaatcgt ttagattggg
    23341 caaaagctgt cgcatttgaa agcaatcgca agcaaagtat tttaaagttt tattctcttt
    23401 ttacaacagt caaaggaatt tctacaaaag tgaaagaaag aacttatcta aatcctcttt
    23461 taaaattagt taagcaaact ccttcaaacc tttggctgtc cctttatgct agagcctttt
    23521 tacgtagttc agattatttg ggcttatttt tacgactaat gctattaagc tcattatctg
    23581 tattttttat ccataatctt tatttgtcag taagtttagc actcattttt aactatttag
    23641 ttgtattcca attactttcc ctttattatc actacgatta ccattatatg actagtcttt
    23701 atcctgaaaa tagtcggagc aagaagaaaa atatgttaag ttttttacga ggtttaagct
    23761 ttttaatgtt aatcgtcaat atgctgtgtt gctcatcagc gccaaaagca ctcattttga
    23821 ttgtaggtat ggttttcata gcttgtatat atctacctta taagttaaag aagatcattg
    23881 actaagtagc ctaaaacaag taaaataggg gtaaacgact ataaaagata gaaaaggagg
    23941 gcgataaggt gacaacgacg gaacaagaac ttaccttgac tcccttacgt gggaaaagtg
    24001 gcaaagctta taaaggcact tatccaaatg gggaatgtgt ctttataaaa ttaaatacga
    24061 cccctattct acctgcctta gcaaaagaac agattgcgcc acagttactt tgggccaaac
    24121 gcatgggcaa tggtgatatg atgagtgccc aagaatggct taacggccgt acattgacca
    24181 aagaagatat gaacagtaag caaatcattc atattctatt gcgccttcac aaatctaaaa
    24241 aattagtcaa tcaactgctt cagctcaatt ataagattga aaacccatac gatttattgg
    24301 ttgattttga gcaaaatgca cccttgcaaa ttcagcaaaa ttcatactta caagctatcg
    24361 ttaaagaatt aaaacggagc ttaccagagt tcaaatcaga agtagcaacg attgtgcatg
    24421 gagatattaa acatagcaat tgggtgatta ctactagtgg tatgattttt ttagtagatt
    24481 gggattctgt tcgtctaact gatcggatgt atgatgttgc ttacctgttg agccactata
    24541 ttccacggtc tcgttggtca gaatggctgt cttattatgg ctataaaaat aatgacaagg
    24601 ttatgcaaaa aattatttgg tatggtcaat tttctcacct gacacaaatt ctcaagtgtt
    24661 ttgacaagcg tgacatggag catgtgaatc aggagattta tgccctcaga aaatttagag
    24721 aaatatttag aaagaaataa tgcgagttag aaaacgaaaa ggtgctgagg agcacttagc
    24781 taataaccca cactatgtga tattaaatcc agaagatgcc aagggccgtt ggcatgacgt
    24841 ttttggaaat gaccgtccta ttcatatcga agttggttca ggaaagggcg gatttatcac
    24901 agggatggcc ttgaaaaatc ctgatattaa ctacattggc attgatattc aattatctgt
    24961 cctaagctac gccttggata aggtattagc ttcagaagta cccaatgtca aacttctgcg
    25021 agtggatgga tcaagtttaa ccaactattt tgaagatggt gaggtggata tgatgtacct
    25081 caacttttca gatccttggc caaaaaccaa gcatgaaaaa cgtcgtttga cctacaagga
    25141 tttcttagac acttacaagc gtatcttgcc agaacacggt gaaattcatt ttaaaaccga
    25201 taatcggggc ttgtttgagt acagtttagc tagtttttct caatacggca tgaccttgag
    25261 acagatctgg cttgacttac acgctagtaa ctatgaagga aatgtcatga cagaatatga
    25321 agagaagttc tctaataaag gtcaagtcat ttaccgagtt gaggcaaatt tctagtagta
    25381 ttttgtttca aaatatgata acataaagga gttgatacag tattaacttc tttagtaatg
    25441 gagaggtcgc atagtggctt agtgcgcacg cttggaaagc gtgtagtcct taacgaggct
    25501 cgggggttcg aatcccctcc tctcctttta tcctcttgat atatcaaggg tttcggggtc
    25561 ttcgcccgtc tttcacccgt caaatttgag attttgaaaa atgtttttga cctgatggtc
    25621 atttttttct tttaaacttt ctatctgatg agcgtaaact ttgagtgtga tgttgagatt
    25681 ttcgtgcccc aacagcttgg atactgatat taaatcaacg ccctgcgcta tcaaataaga
    25741 agcgtaagta tgccttagcg agtgattggt tagatttctg ccagtcactc tttttataac
    25801 tttgttcgtt gcattatttg acgctccaaa gcatacacga ccgagtttat tatcctgata
    25861 atagtcgctc ttaaaatttc ttagtatctc aaggctattt ttatcaatcg gtaccttcct
    25921 gatggactgc tcgtttttgg tcggaccgaa attttgagaa attgaataat caaaagtttt
    25981 attaatatcg atatagccat tctcaaaatc aatgtctttc caagtcaacc cttgaacctc
    26041 tgcaaatcgt aaaccggtga cggctatgat atacgttgtc aggtatgatg ggtacttgac
    26101 tttgcttttg gcaaccttga taaaggcgag gtattctgat tcttccatga atttctctga
    26161 ctcttcttta gcgggttttt gcgatctaac aatggcccca tcggcaaaat tatccctaat
    26221 aatgccatca cgaatggcca ttttacaagc acccttgatt tgataatgaa atttatccag
    26281 agtttgttgg gctacttttg cgccaaagtc attaaggact tgctgataaa aggtgtgtgt
    26341 gatactcttg agcttccgag tcccaaaata atgttcgatg tgcttaaaat ttttggtgta
    26401 tgtctgccat gtctttgcgg taatgtgagg ccttttatat atgtctgccc aacgtctgtt
    26461 gtagtctaaa acagtaatat ctaaaaggtc tgcggtcagc gtatttaaat caatttctgc
    26521 ttgctgagca gcgacttttg caagtgtttt tgttttaaat ccacgttttg atttttctct
    26581 ttttttacct tgactgtcac gataggtgat gcgatactcc cacccagaac ctaattttcg
    26641 ataaaatgcc attgttttca cctcattttt ctgttaaaat gggtacaaga aaacgacctt
    26701 tttaatggtt gtttcttata cggttttgcc tcacgctctc ctcgccaaat ttgagcgtgg
    26761 ggtttttttt atttaaagaa attattaaca cttacatact gagcctgcgt aagcttagtt
    26821 tttaaggttt taacttctcc ggtttgtata tttcttaagg ctaccgttgc ttttccaggt
    26881 atttcttcag aagtggttgt ggcagtagtg ttaatggttc cttttttctt tctagcccca
    26941 ccagccattc cgccgataat ggttccaacg cctggtgcca atactttacc tattgctgcc
    27001 cctcctagaa cactactaga acgccctttt tgagtagttt tcccttttgt tactgttttt
    27061 tcaataattc gtgacccatc aaattgaagg ctaacaaact cataaagctc aggagtttca
    27121 gaataaaaac cgatataata ttttccatct atggtttttc gtatagccgt ttgtgctcct
    27181 agttctaaat ttagggcagg gggtaggatt attttgctct cttgtgaggc tttttttctt
    27241 agttcgttag ctttaattgc accgtcttta gctttccctg cagctgttat ggcaaattct
    27301 tttaacttag ctatatccat gcttgtctcc ttaactaatt aaaaactcta attcttcttt
    27361 gatcatcact tcgtcagcga ttgaggtcag ttcatacttt tccatgaatt ggatgtagtt
    27421 gaaatcctcc atattatccc aataggatag ctcttctttt aagagatggt gtatcataca
    27481 tctatctgct tgtagctctg ctttttcttt gtttagtttg taataactcg cagtatgttc
    27541 tctatgtcct agttcatgat agatgacttt gtgtttatat ataccgtcaa gataggtgtc
    27601 aatcgcaatg acgttgtgtt ttttattaaa cattccaggg gtgtttgtac ccctaccgtc
    27661 aaagtacact aaatcgattc ctttttcctg acagactgtt tctggtgtca tcatagagca
    27721 gtcctctcta ttttctattt ttgatgcgcg tttccaatat agatgtgata aggtctatat
    27781 cctcgtcgtt gagttcgtga ccgtcgaaga agaaagtctc ttctgcatct ttttttagat
    27841 caattgcgac cgaagcgtgc ccatcttgcg caatcttagg attatcagtt cgacctaata
    27901 ggtaatcggt tgatacgtga aagtagtcag cgattagttg tagtctttct gctgaaggct
    27961 ttttattttt aattgtatag attgaatttc tactcaatcc caatttttcc tcaagcaatc
    28021 ccaaggaaat acctcttgat tttgcaagtt ctttaattct ttcgaatgtt gaaaacattg
    28081 atttgtcagc ctttctatga gatgacaaaa aatatctata aaaaagcatg taaactactt
    28141 gactgattac aaac
//
LOCUS       DAXWIQ010000021        27958 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000021 DAXWIQ010000000
VERSION     DAXWIQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27958
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.021"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            22..1194
                     /gene="sda1"
                     /locus_tag="ACGEUI_000935"
     CDS             22..1194
                     /gene="sda1"
                     /locus_tag="ACGEUI_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptodornase Sda1"
                     /protein_id="HFF8165779.1"
                     /translation="MSKHWRHLIIHSALTILATLFLGILPITENTSSTIYAESGTISN
                     NWSIEQHPNYYHVEGKAQLDIKNFPELYRTTERVYKKSGQSTKPVTVSNIHYSVLDGY
                     GRSGEAYGIITKDMIDMSAGYREKWESKPEPSGWYSYFFKNTNQRATESDYKHSPKNV
                     SKISNNIKASILLSNGNVRNGYLFDRSHLIADSLGGRPFRNNLITGTRTQNVGNNDRK
                     GGMQYIENKVLDHIKRNPKVHVYYKATPVYQGSELLPRAVLVSALSSDGFIDETVRVF
                     NNVAGFNIDYQNGGLLSSTADVDINNVEENEIETTDDEIEEGIENEPDTDALKKDNKD
                     TSLQDTVYVASNGQSDVYWYNKDSMPKTVNLEKVVEMSEQVALTRGKHHSTQENIE"
     gene            complement(1310..2506)
                     /locus_tag="ACGEUI_000936"
     CDS             complement(1310..2506)
                     /locus_tag="ACGEUI_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8165780.1"
                     /translation="MTFLDNIKQGCLDGWTKYKILPSLTAAQAILESGWGKHAPHNAL
                     FGIKADASWTGKSFNTKTQEEYQPGIVTDIVDRFRAYDSWTDSIIDHGKFLNDNPRYQ
                     SVVGETDYKKACHAIKDAGYATASGYAELLIQLIEENDLQKWDKEILTNQKEVTMTTA
                     NEIVKYCVDLANSGMGVDKDGAYGTQCCDLPCFIVKNWFGIDLWGNAIDLLNSASAQG
                     LEVIYNAPGVNPKASDLFVMEVAGSPYGHTGAVIEDSDGYTIKTVEQNIDGNWDSLQV
                     GGPARFNTRDFTGVVGWIRLPVDHAHQTVDTAPQNSDTIVETPKSGTFTLDVVEINIR
                     RWPSLASEVVGSYKQGDTVSFDSEGYANGYYWISYLGGSGMRNYMAIGITDKDGNIIS
                     LWGKLN"
     gene            complement(2617..2802)
                     /locus_tag="ACGEUI_000937"
     CDS             complement(2617..2802)
                     /locus_tag="ACGEUI_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFF8165781.1"
                     /translation="MSKWFKKVGIKTIKTMAQTAVGLIGSSVLITDINWPTMLSAVLL
                     SGLTCVLMNVSQIKDEE"
     gene            complement(2799..3098)
                     /locus_tag="ACGEUI_000938"
     CDS             complement(2799..3098)
                     /locus_tag="ACGEUI_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165782.1"
                     /translation="MHFDLLQAGAAAAALMSVFGLWKAVVTPFKKAMEANEFAMAQLK
                     DSLKELAYELKNLDRDREITKKIIDRHEERLGRVEDEVIINKERIITLFKKGEEK"
     gene            complement(3109..3729)
                     /locus_tag="ACGEUI_000939"
     CDS             complement(3109..3729)
                     /locus_tag="ACGEUI_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165783.1"
                     /translation="MRNWKVTGNYPQWDGTGAVVDTQVIITDDRGAVISEKVKKDLRT
                     ANDAEIIEAALEEFKKTAYVEIAMGEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVS
                     AERTQKMINLQTIHVLTSGGKIDPDIYKGMLELIEPAKKGEYKEHDIFTVVDADKDTD
                     GVAGEGNLVFVYVNDKFTYDAQTLKDLEELEDKVTVIKYADLVKQD"
     gene            complement(3732..3893)
                     /locus_tag="ACGEUI_000940"
     CDS             complement(3732..3893)
                     /locus_tag="ACGEUI_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165784.1"
                     /translation="MQEKLLGKIINDLTLKVANLTLENAQLKAQHEIELEELNAQLDE
                     ATAPKEEGK"
     gene            complement(3902..5809)
                     /locus_tag="ACGEUI_000941"
     CDS             complement(3902..5809)
                     /locus_tag="ACGEUI_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HFF8165785.1"
                     /translation="MSRDPTLLIDESNLTIGSDGRAYYTFKADGDTKSVKLANNKCIG
                     TTRFNQLMIERGGKPTNYVAPVVVEGSGESTGLFKNLEGALSQLKELNLELTDTVNSQ
                     LWAKIKLTTNGMLREYHRDNITTEIVESANGIATRISEDTDKKLALINETVSGIRRDY
                     QDADRQLSSTYQAGINGLKATMANDKNDLKAVIQATAQGLSQKYDDEIQQLSAKITTT
                     SSGTTEAYESKLADLRAEFTRSHQGMRTVLESRISGLQSTQQSTAYQISQEIKNREGA
                     VSRVQQDLASYQRRLQDTEKNYSSLTQTVRGLQSTVSAPNRGLESRVTQLAGLIEQKV
                     TRGEMESYIRGAGDSIMLAIKGKLPQSKMSASEIVSAINLNGYGVRISGERIALDGNT
                     TVNGAFGAKLGEFIKLKADQIIGGTIDANKINVINLNASSIVGLDANFIKARIEHTIT
                     SLLEGKVIRARNGAMMIDLNNSTIDFNSDASINFNSNNNALVRKSGTHTAFVHFGNAT
                     PKNFTRSALYASIGITSSGDGINSASSGRFCGARFFRTASGYEHTASVDQAEIYGDTI
                     IFADDFGINRGFKMTPTGVNTLVDINKMYYAIVALARCWKHLQNVGWDTTHPNFTSAI
                     MNEHSNYMTGI"
     gene            complement(5820..6455)
                     /locus_tag="ACGEUI_000942"
     CDS             complement(5820..6455)
                     /locus_tag="ACGEUI_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165786.1"
                     /translation="MAVQEIPDTYFYRLDSQSIDGTLLQKASLVVDQFYIKIDDYYSF
                     YTDQDVDVYKIISSTQADVYKANKYNYQSLSKPDTSVQKPSAAMGLYQSITIDGLPIA
                     RVICNNQVIWSQSAEPQEVYNITAYVDANVSKLNDYAVIDYNQDFATYGVVWHRVDKV
                     AVEVDGKIVAKLQKPVRTVAGGGAMLSIETWTYPFDGIDKYKNPTFKISQH"
     gene            complement(6455..7510)
                     /locus_tag="ACGEUI_000943"
     CDS             complement(6455..7510)
                     /locus_tag="ACGEUI_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronoglucosaminidase"
                     /protein_id="HFF8165787.1"
                     /translation="MTLEERIPIKVLFDRKDAAEWQKLNPVVDDGELVVELDTHRLKV
                     GDGKLNYNDLPYYEGPQGESITKVQLSENGDLSVWIGDKETKLGNIKGQKGDKGTSIA
                     DITKVGETLTITLSDDTQKTFSIPNGQKGDRGKGVESARVDEEGNLFVKFEEESEKLV
                     GNIKGQKGDKGDSLKFEDLTSDQIAQIKAKDVDLSAYATKAELKEIDVSKQLADYLSK
                     AEANSTYAKASHKHSLSDITDLNLDRYATKIELQNKASTYHRHRTSDIDGIDEYLKQT
                     DLPQDIVKQSDIRDVVRNAQLSGYVRLADIQYQLNNIGKLKDVATGQYLSVRVVDKGK
                     VPYNTSGLIVFERSGGK"
     gene            complement(7507..9489)
                     /locus_tag="ACGEUI_000944"
     CDS             complement(7507..9489)
                     /locus_tag="ACGEUI_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8165788.1"
                     /translation="MITFLDHRDIEYGAISVIRHTNAVNGERSVSGEIYTNSDVLNNI
                     DRGWRLRFEDEYYVVIYAKPVDVGQKTQVSFDAIHQFFWDFSKSSIYEGLGDGSHTID
                     TYLETVFKGSGYRYKLEVGVNAFRKQSFNYKSRLDLFNEIIKATGLEFSVSGKVVRLL
                     KNIGTDLSTVVRKNFNMNDLTIEKNIDSFITYQKGFGAWTDPEDHSKGRLEAEYESPL
                     AKEYGRLEGAPLTDERFTVADNLKEALKSNVENSYKISVKIDMEDLMRAGYRCERPVA
                     GDYIMAINETLGFQERIRIVSFTSYYDATGALVKHEVTCNDIGSVKKQSVGSLSINSR
                     INQIDADIASAIEVATQALVSADGKNTVYGGTEMPKDEPKGTLKKGDILFLKVGDTTR
                     MYFWNGAEWEEPEVVNDPERWREDLEKQISEAIEKAKKAQEEINRRTDKELEEFRETL
                     KNLALPEEAIKKITDAIKVDDIPSIKQSFDDLKNKVSETSETSRLNAEIIGTDGKTRY
                     NKNLLVGNPNRVKKIDEDFIEVEANDGGFKRGETYTISFSQTCEPLNKVAITLIQANN
                     KGLKLVLTPTKAKMEPQTFEVTKDKQSIEVYPLSYKGVLTGDWYKSKQIDLTASEGQI
                     LALDMAYKAVVDAKGATITAKQSSNPKIILDGRRDR"
     gene            complement(9499..10341)
                     /locus_tag="ACGEUI_000945"
     CDS             complement(9499..10341)
                     /locus_tag="ACGEUI_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HFF8165789.1"
                     /translation="MDLLIEKESQATRLSDFGIYNIAIEDSAPLLSVSHRAVKGRSGY
                     IYDGATFTTKTLKVKGRVTVSDVEGVLDKQDELNALLVVDEPFYVTKMYPENSDLFNF
                     ELPGESTGDLQLIGSPHKPWKYRFKVILDDTIAYEFIGKTSQGLKYNLSFTLRTAELP
                     FGETKPKDITLSGGSFAYGGTAKASQLEWPFIIELTPSGGQTNFYIEIDGRRFEFKQN
                     SQLQNSDKLLLTGIATTLNGNYVNAKTNYEYFIFNPNPNKRITYKTDFLGTIRILNFV
                     ELYK"
     gene            complement(10353..14735)
                     /locus_tag="ACGEUI_000946"
     CDS             complement(10353..14735)
                     /locus_tag="ACGEUI_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFF8165790.1"
                     /translation="MPGTFDGSIFADIGANTKDYEQAMARIVSTTQNAFRKAQDTAVN
                     SSNKMVQIIGQIMAQLANNGESLGKRLGSAYATGLKLSIGEIQRIAASIGEKIPEPIK
                     NGFQKIIIPVASVIDKIRGNLTGFINQTKMQLQNLVDIGKIRSAFAKAATGVDILTQR
                     ASSKLNNLSAIFETVAGKLPRPFGTAFRKIASSVSTLNSSIQSVGGKISNSLGRQVLN
                     PALQSWNNFFSSVSAKASAFANKVSTSLFGRLTSSLANLSSKIGSSLSNGFSRMSSSA
                     ATSLNGISQKFANTSSAGERLKSTVMSIVQAFSLMAVVQKAMHAITGAIDGAVSRVDT
                     MNRFPKTMALFGYSAEQSKASIDKLSKGIEGLPTPLDSAVKSAQQLAITTGSLDKGTS
                     LALAFNNAMIGYGATTEGAEQALRQFNQSLGSGKIQAEEFNSVSEAAPGLMSKMAEAF
                     GFGKNGVQDLKSALSDGKITAQEFADKMIELNDAQGGFAEMAQSSAGGIRTAWKNVHT
                     AVVKGVAGMISAFDEAAKANGMKTIAETLLSLKPAITSVFDTINSLIPNAVAAFARLK
                     QSINIDFSPLGASVKEVFALINTVLGDFAHTGELSGQAFDNLKAKITSLAPKVIALWA
                     VMNPASAIATIMPLLSLFGKVGLALGSLGASVGVFGGIISSGIASASGVVGAFAATLS
                     GLPGVFATAAGRGLSVLGTMTSAMSSLVSLALAAIGPAAILGLVVAGLGLINSQFGAQ
                     IDQLLNTAVTKGPSIIQGLVKGITSKIPALIASGTQLIAKFANAITVLLPVIIQAGVQ
                     LITSLVQGIGQNATSLISSAIKIIGSFVSSIASALPQLISVGMELLLNVVNGIVQNIP
                     LIIQQAQQIIGSFGNSLQANLPSIISHGIAILVNLVQGITQMLPTVLQIATQVITSFV
                     SGIVQFLPQLLQGGIQIIISLVQGIIQNLPQIVQSAVQIIQSLVSGLTQALPQIIAAG
                     IQLVVQLAVALIKGLPQIISAGIQLITGLGKAMLEAIPNALSGVWEGIKSGFSSMWDQ
                     ITGKSSTSTAKVSADATAMALNVGTQTTAMANQANTDTTSMLNSISQNTELANINATS
                     NATQMANNVNAQTGTMSLQGLNDSMSLASGISANMSVANINATTQAQEMASGVNGATS
                     SMNLDAINHTLSLASGVGANMGTASTSATSQAQAMSSGVSNSLASMQSNSTKAASGLS
                     NSVTSEMSSAATSATSSANRLSSAVESGFNKAKTSATTSMNGIANAVKTGFSSINSTA
                     KQSMANLVSSVTSGMSRATAVSNNACQKILSIFRALAGQMSSAGAYAGQGFANGLASS
                     AGTIYAIANSIAANVAATIRRALDIHSPSRVTKTLGAFTGEGFALGMAEWIGEINSLG
                     KAYATAVTDQNWGVNSTVSTSAKVNNSGINTSLDNLSEEVRQSQLSEPIFEVHNEIVG
                     DKIYTAVKEKESREQSKDSYFVFA"
     gene            complement(14750..14983)
                     /locus_tag="ACGEUI_000947"
     CDS             complement(14750..14983)
                     /locus_tag="ACGEUI_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165791.1"
                     /translation="MEGLEYKQVAERENLSELSLKLRYTLNAKKVDVGKLKYDKHRLT
                     IKRSYQKASRSQADSDSSIVERIQMLNNHFQNR"
     gene            complement(15058..15513)
                     /locus_tag="ACGEUI_000948"
     CDS             complement(15058..15513)
                     /locus_tag="ACGEUI_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFF8165792.1"
                     /translation="MEFTAAKRNIDIKFDFKTMFKINNKLGTINPETGERNADGVGAL
                     FFNILERNESAIVDLVRLSAGSGKKALTEDEILDAIAEAVDEEGTTEGLFAEIEKEMV
                     DSGFFRAKILKYIENMEKSARYLKAKDDTDATQIQIIEDMIGRMSNAVS"
     gene            complement(15567..16166)
                     /locus_tag="ACGEUI_000949"
     CDS             complement(15567..16166)
                     /locus_tag="ACGEUI_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HFF8165793.1"
                     /translation="MANTKEGTTIEITTGKPIVGKKIFYFIQSVDAKKGSKALLPAYR
                     TDGTTTMGGEYIDEQTQQGRVIEKATDEHSIDLTTYFVPTDPSVAVIEEAKKTGKSIK
                     IWEVIADESVKEQIQISESAGPKKDVYPAKFGYAKIDEIERGTGISDLVEMSYTANIV
                     GALQDGKFPLTKEEIEMLENVYGYQNPGDTTGDYDNITK"
     gene            complement(16178..16537)
                     /locus_tag="ACGEUI_000950"
     CDS             complement(16178..16537)
                     /locus_tag="ACGEUI_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165794.1"
                     /translation="MDYSPETLYLKKVKNRLGALGIPIYFKLPKSDVLEPFIVVGTNI
                     SDLSKTAQTGAVIDDFSLNIDAFLPGDSRLDAEEIKARLLRLLGRNNQIKAQILVDNS
                     IGREVYRVAINITETLF"
     gene            complement(16541..16885)
                     /locus_tag="ACGEUI_000951"
     CDS             complement(16541..16885)
                     /locus_tag="ACGEUI_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFF8165795.1"
                     /translation="MSLIYRMRGLDRFLRSVERKQKSVRIAVDKELSKSAARIERQAK
                     ILAPVDTGWLRAQIYNEQQRLLHYRVVSPALYSIYLELGTRKMEAQPFLDPALRKEWP
                     VLMANLKKMFKR"
     gene            complement(16882..17160)
                     /locus_tag="ACGEUI_000952"
     CDS             complement(16882..17160)
                     /locus_tag="ACGEUI_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165796.1"
                     /translation="MIYKDRVILVYVDEQDDFLDKRTVEKPSGKIPCMENTFTKSEQM
                     GLFGKYDLNAFKLHLQGHYDGFSKIIYKGKPRLIKGLAHHKNSTVIYV"
     gene            complement(17171..17527)
                     /locus_tag="ACGEUI_000953"
     CDS             complement(17171..17527)
                     /locus_tag="ACGEUI_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFF8165797.1"
                     /translation="MGDKQLIDDIKLFIGISKDDGAQDELITLAISESKERVLAKLNE
                     YSETEITKIPDRLRFIVRDVAIKRFNRINSEGTVEDSEEGKTFKWDSYLKEYESTLRS
                     AAIGKVYSGKGVARFI"
     gene            complement(17539..18426)
                     /locus_tag="ACGEUI_000954"
     CDS             complement(17539..18426)
                     /locus_tag="ACGEUI_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFF8165798.1"
                     /translation="MAEKNLNTMADLGDIKSIDFVNKFSKNINDLLKLLGVTRRETLT
                     NDLKIQTYKWEVTLDQTDPGEGETIPLSKVTRTKDKDYTVKWFKKRRATTAESIARHG
                     AARAITEADKRIMRELQNGIKDAFFTFLKTKPTKVKGVGLQKALSASWAKLATFNEFE
                     GSPLVSFVSPLDVANYLGDTKVGADASNVFGMTLLKNFLGMQNVIVMPSVPEGKIYST
                     AVENLVFASLNVKGGDLGGLFADFTDETGLIAAARNRQLSNLTYESVFFGANVLFAEI
                     PEGVVEATIEAAAVPGIGG"
     gene            complement(18439..19008)
                     /locus_tag="ACGEUI_000955"
     CDS             complement(18439..19008)
                     /locus_tag="ACGEUI_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFF8165799.1"
                     /translation="MAEENVTTETTEQVDTQKEAVEHPKQEHERTFTRADISKMMAAE
                     RTKWESEQSEAIEKARTEGERLAKLSKDERAKEEEQKRLDAIAEREKAVAEREMRIET
                     HSLLVEKGLPLDFIDIVLTTTAEEVKANIDNLQTIFDKAVEKRVNDRLTQKPPRTGNG
                     SVGMTKADIMAIEDDDERMRLIAENRNLF"
     gene            complement(19164..19430)
                     /locus_tag="ACGEUI_000956"
     CDS             complement(19164..19430)
                     /locus_tag="ACGEUI_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165800.1"
                     /translation="MNKRIKKKRKLETVVVMLVAENAMQAEAIKNQNKEIMELKSIVQ
                     RNALATNEEFASVKAATLDNQAAITNIAIDVNYIKKNYKRKWGK"
     gene            complement(19433..19621)
                     /locus_tag="ACGEUI_000957"
     CDS             complement(19433..19621)
                     /locus_tag="ACGEUI_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165801.1"
                     /translation="MGVRPSSFFISIIHHPIKTIKEENMFIWQMVLVALGVLVLILIV
                     GIAAIAVKSIIAELKKVG"
     gene            complement(19652..21097)
                     /locus_tag="ACGEUI_000958"
     CDS             complement(19652..21097)
                     /locus_tag="ACGEUI_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFF8165802.1"
                     /translation="MLVVKKNQQQKSLNYWQKRQEDILSYLDRADLDVFSELQKLYNE
                     QAFELQKELFDFYTKYSEENKMTYQDVVKKLRHEDLSDYVANANKYRKQAEKDPELLK
                     RLNEQYVSARATRMDALNLELVYRAGILKGVLDSAFENHLKKAASYAYKKAMGGRSGT
                     INGPVLEELVRTPFDGYNYSEQLWGNTDNLVKNLQKKLKQGFVRGEHPRAIARDLAKQ
                     FNVANHRAETLIRTDGTMVINNATARRYLDAGLKYYRDLVRRDDRTTEICRKIAKENK
                     RKLLSELKPGINAAPYHFNCRTTLIPDEDELSIEVEPIDDKSTKYFKDVTSDWIDGNG
                     HKPQLSLLNEYVKNGTPYKVDGHNVVLDHSNYEYRVANWLSKKTGLQVDMVPRVNSPE
                     HINTPDYLVDRVPFDLKEITGSGKNVIDGNLRKAKKQATNIIFDITKTPLSFEEIMGQ
                     LEQIYMIDRRGLDISIIKNKDEVLAVLEKEG"
     gene            complement(21057..22589)
                     /locus_tag="ACGEUI_000959"
     CDS             complement(21057..22589)
                     /locus_tag="ACGEUI_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFF8165803.1"
                     /translation="MEEKQFLAGTRFNENANKQFIMLQEDFEAIDYESKLWIDQLKNY
                     ISRFKAEQLERLKELKRYYLGDNNIKYRPAKTDKYAADNRIASDFAKYITVFEQGYML
                     GVPVEYKNENKDLQAAIDLMSVRNNEDYHNVKIKTDLSIYGRAYELLTVEEIDDKKTE
                     VKLYQLPAEQTFVIYDDTYQRNSLMAVHFYNIDYGSGKRKQIIKVYTSDTIYTYEDYN
                     LETKGTCLKEHEEHFFKGVPVNEYANNEERTGAYESVLDNIDAYDLSQSELANFQQDS
                     VNALLVIAGNAYTEADENDYLDDGRLNPNGRLAISIGFKKAQVLILDDNPNPNGVKPQ
                     AYFLKKEYDTAGSEAYKNRLVADILRFTFTPDTQDMKFSGVQSGESMKYKLMASDNYR
                     EKQERLFKKGLMRRLRLAANIWAIKGNEATTYSLVNDTSIVFTPNLPQNDNEIVTAAQ
                     NLYGIVSDQTIFEILNTVTGVDAEAELERLKEEADKKQSLPEPRLVGDASGQEEPTAE
                     KP"
     gene            complement(22605..23882)
                     /locus_tag="ACGEUI_000960"
     CDS             complement(22605..23882)
                     /locus_tag="ACGEUI_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFF8165804.1"
                     /translation="MTIKQRPKINIVIKYPSKVFNKHIYDKLYNYSNFTEVHYGGASS
                     GKSHGVFQKIILKALNPKFKHPRKILVLRKVGATVRDSVFADIMSNLSYFGILDKCKI
                     NMSAFRITLPNGSEFIFKGMDNPEKIKSIKGISDVVMEEASEFTLDDYTQLTLRLRDK
                     KHLEKQIYLMFNPVSKVNWVYKAFFVKTPKNTVVYQTTYKDNRFLDDVTRENIEELAD
                     RNEAYYKIYALGQFATLDKLIFPKYDKQILNKDKLSHLSSFFGLDYGFINDPSAFLHV
                     KIDDANKKLYVIEEYVRKNLTNDKIANAIKDLGYAKEEIRGDSAEKKSNQELRNLGIP
                     RMIDVTKGPGTVMQGIQYLLQYDWVVDERCVKTIEELENYTWKKDKKTNEYINEPVDS
                     YNHCIDAIRYAVQDRIYQSADRSKRMKNAKYYF"
     gene            complement(23872..24324)
                     /locus_tag="ACGEUI_000961"
     CDS             complement(23872..24324)
                     /locus_tag="ACGEUI_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFF8165805.1"
                     /translation="MLGGDGKLSKLTLKQKRFADEYIISANATAAAIKAGYSKKTARS
                     IGQENLTKPDIKAYIDERLEKLESEKIATQEEVLQYLTSIMRGDQQEKTLISVGEFGQ
                     KIVDIDVGAKDRIKAAELLGKRYRLFTDKVEMDVSSDVTINVGEWDDD"
     gene            complement(24414..24830)
                     /locus_tag="ACGEUI_000962"
     CDS             complement(24414..24830)
                     /locus_tag="ACGEUI_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF722 domain-containing protein"
                     /protein_id="HFF8165806.1"
                     /translation="MSRLSNAQLKAFDEWLFDYRFIDNKIALRKLELQTDMNTEVDKN
                     IGGGRANFVSKVTEDVVARWDSDRELKGLSNFKEAVIATLNLLDEELTDIFNLRWGVG
                     SSNTWEEIAYTRHLSRKSIYRKRERILEIFSKKIGM"
     gene            complement(24827..25018)
                     /locus_tag="ACGEUI_000963"
     CDS             complement(24827..25018)
                     /locus_tag="ACGEUI_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165807.1"
                     /translation="MKIEFFLKQGPTKSVSNYGEICIDEVIGAMKNNDVIALNGKNNA
                     VIIPKQNISHVIIHKEVTG"
     gene            complement(25008..25859)
                     /locus_tag="ACGEUI_000964"
     CDS             complement(25008..25859)
                     /locus_tag="ACGEUI_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFF8165808.1"
                     /translation="MEIKCGNCLELLAELTDGLIDMVITDPPYNISVKNNFATMGRTG
                     IDFGDWDKGFDLLSWIDIASQKLTKNGGMIIFNDWKNLGDIARHCEKNGLVVKDIFRW
                     VKDNPMPRNRDRRYITDSEYGVWVVRKKSKWVFNRKSEKYDRPEYRYPVVAGAEKTQH
                     PTQKPVALMKDIITRHTTKGAVVLDPFMGSGSTGVACLLTGRDFIGYELNKDYFNIAN
                     KRIAELTGNGGVGMNIIHRMENGIDAVGDLTTYAVPCCPECKNETYSQKHCPFCGENL
                     MEVANEN"
     gene            complement(25868..26134)
                     /locus_tag="ACGEUI_000965"
     CDS             complement(25868..26134)
                     /locus_tag="ACGEUI_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165809.1"
                     /translation="MTEELIKPLLEEIKRKDDRLYMQSNVIAVLMTAVIMLLMISIAL
                     QNHYEPQITGLRTQLSRTQKQLKRASEDRARQTKRIAELTGNGG"
     gene            complement(26131..26298)
                     /locus_tag="ACGEUI_000966"
     CDS             complement(26131..26298)
                     /locus_tag="ACGEUI_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165810.1"
                     /translation="MKKEYVVRIYTGREKNFEAKPQFEEKTFTRKADMLKFWNSCEAT
                     VKEKYTREWKK"
     gene            complement(26299..27621)
                     /locus_tag="ACGEUI_000967"
     CDS             complement(26299..27621)
                     /locus_tag="ACGEUI_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFF8165811.1"
                     /translation="MRLHEYQEYAKTWIVEHPYCGLLLDMGLGKTLTTLSAIDEIQNI
                     FSEDHKILIVAPKKVAEETWPTEIEKWHFDFTYSKVLGSEGKRIEALETEADIYLINR
                     ENVTWLVEYYKTKWPFTFVVIDELSSFKSSKSKRFRALRKVRPKVQRLVGLTGTPAPN
                     SLIDLWPQIYLMDRGDRLETSQTRFKDKYFVPDKRNGPIIYSWALRDGAEAEIYNKIE
                     DICVSMKAKDYLKLPPRTNNVVSVKLSNMKAYKQLEADLVLEFKDKEISAANSAVLAN
                     KLLQMANGAIYDDDKSTVAIHDDKLDALEDIVEESQGQPILVFYQYQHDFERLKKRFP
                     QAEELTSVDKWNSGKIPILLCHPQSAGHGLNLQKGGHIIVWFGLTWSLEYYQQANARL
                     DRQGQTEPVIVHHIVAENTVDEKVLRILQGKEKNQNALLEAVKAQLGV"
     gene            complement(27618..27893)
                     /locus_tag="ACGEUI_000968"
     CDS             complement(27618..27893)
                     /locus_tag="ACGEUI_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFF8165812.1"
                     /translation="MRTEKDIENYLKKKTKGLCLKFTSPGTIGVPDRIVVMNTGTFFV
                     EVKAPGKKPRPSQVAMHKKIKEAGQHVWVVDSYESVDMALKEMENWV"
BASE COUNT         7580 a         6240 c         4899 g         9239 t
ORIGIN      
        1 taacaaaagg aggacataaa tatgtctaaa cattggagac atctaattat tcactctgct
       61 ctgaccattc tagctacgtt atttttaggt atactaccaa ttactgaaaa tactagtagt
      121 accatttacg ctgaatcggg cactatttca aataattgga gtatcgagca acaccccaat
      181 tattaccatg ttgaaggtaa agcgcaactg gatattaaaa attttcccga actttatcgt
      241 acaaccgaaa gggtctataa gaaaagtggg caaagtacta aacctgttac agtttccaat
      301 atccattact ctgtacttga tggctacggc cgttctggag aagcttatgg tattatcaca
      361 aaagatatga ttgacatgtc tgctggctat cgtgaaaaat gggaaagcaa accagagcca
      421 agtgggtggt attcttattt cttcaaaaat actaaccaga gagccactga atccgactac
      481 aagcatagcc ccaaaaatgt gagtaagatt tcgaacaata tcaaagctag tattctctta
      541 agtaacggaa atgttcgtaa cggctacctg tttgatagaa gtcatttaat agccgatagc
      601 ttaggaggaa gaccttttag aaataatttg attacgggta cccgcaccca aaacgtaggt
      661 aataatgatc gtaaaggtgg gatgcagtat attgaaaata aagttttaga tcacattaaa
      721 agaaatccta aagtccatgt ttactataaa gcaactcctg tatatcaagg atccgaattg
      781 ctacctagag cagttttagt gtctgcttta tcatctgatg gatttattga cgagacagtt
      841 cgtgtgttta ataatgtagc aggttttaat attgattacc aaaacggtgg actcttatct
      901 tctactgctg acgtagatat taataacgtt gaagaaaatg aaatcgaaac tactgatgac
      961 gagattgaag agggaatcga aaacgagcct gacacggatg cactaaaaaa agataacaaa
     1021 gatacttctt tacaagacac tgtatatgtg gcaagtaatg ggcaatctga tgtatactgg
     1081 tacaacaaag acagtatgcc taaaactgta aacttagaga aagttgtaga aatgagtgaa
     1141 caagtagctt tgactagagg taaacatcat tcaactcaag aaaatataga ataacaaatt
     1201 caacagacca actagaactt gaaatagttt ttatattctt attagttatg cataagcata
     1261 cgaaacacac aaaaaaccgc cctcaatcaa gagagcggtt ttgtcttatc taatttaatt
     1321 taccccaaag gctgataatg ttaccatctt tgtcagttat cccaatagcc atgtaatttc
     1381 gcatacctga gccgccaaga taactaatcc agtagtagcc attggcataa ccctcgctgt
     1441 caaagctgac agtgtctcct tgtttgtagc tacctactac ttcactggct aggcttggcc
     1501 aacgtctaat attaatctcc acaacatcaa gcgtaaaggt accagatttt ggtgtctcta
     1561 cgattgtgtc agagttttgt ggtgctgtat ctaccgtctg atgagcatgg tcaacaggta
     1621 acctaatcca gccgacaacg ccagtaaaat cacgagtatt aaaacgggct ggtccaccta
     1681 cttgtaagct atcccaatta ccgtcaatat tttgctcaac ggttttaata gtatagccat
     1741 cactatcctc gatgacagct cctgtatgtc cgtagggact accagctacc tccatgacaa
     1801 aaaggtcact agctttggga ttgactccag gggcattata tatgacctct agcccttgcg
     1861 cagatgcgct atttaacagg tctatggcat taccccataa atcaatgccg aaccagtttt
     1921 tgacgataaa acatggtaag tcacagcatt gcgtcccata agcaccgtct ttgtcaactc
     1981 ccatacctga attggcaagg tcgacacagt attttacaat ctcatttgcg gttgtcatcg
     2041 ttacctcctt ttgatttgtt aagatttcct tatcccattt ttgtaggtca ttctcctcga
     2101 ttagttggat aagcagctcc gcatatccac ttgccgtggc ataacctgcg tccttgatag
     2161 cgtgacaggc ttttttatag tcagtctcac caacaacaga ctgatagcgt ggattatcgt
     2221 ttaaaaactt gccatggtcg ataatgctat cagtccagct atcataggcc ctaaatcggt
     2281 ccacaatatc cgtgacgata ccaggctggt actcctcctg cgtcttagtg ttaaaagact
     2341 tacctgtcca gcttgcatca gctttaatcc caaacagagc gttatgtggg gcatgtttac
     2401 cccacccgct ctctaagatt gcttgtgctg cggtcaagga tggcaagatt ttgtacttag
     2461 tccagccatc taagcagcct tgcttaatgt tatctaaaaa ggtcatctgt cctccttatc
     2521 tacaaacggg taaaagataa gagcaaccac agataatggc acatacagta ttgcgattgc
     2581 tagtattaac gctaatcgtg tgattgctcg catggcttat tcctcgtctt taatttgtga
     2641 cacgttcatc aggacacatg ttagtcctga tagcagtacc gctgacaaca ttgttggcca
     2701 gttaatatcc gtaatcaaca cgcttgaccc gataagacca accgcagttt gtgccattgt
     2761 tttgattgtt ttaattccta cttttttaaa ccatttgctc atttttcttc ccctttttta
     2821 aatagcgtga taatacgctc tttgttgata ataacttcgt cttcgacgcg acctaaacgc
     2881 tcttcgtgac gatcgataat ttttttggta atttcacgat cgcgatcaag atttttaagc
     2941 tcataagcta actctttgag agagtccttg agttgagcca tggcaaactc attagcttcc
     3001 atggcttttt taaaaggcgt tacaactgcc ttccaaagac caaaaacaga catgagagcc
     3061 gctgccgcag ctcctgcctg tagtaaatca aagtgcatct aaccacctct aatcctgctt
     3121 aaccaaatca gcgtacttga taactgtgac tttatcctct aattcctcta aatctttgag
     3181 tgtttgtgca tcataagtaa acttatcatt gacataaaca aagactaagt tcccctcacc
     3241 ggctacaccg tccgtatctt tatcagcgtc tacaactgta aaaatatcat gctctttgta
     3301 ctcacctttt ttagcaggtt cgatgagctc taacatgcct ttataaatgt caggatcaat
     3361 cttgccaccg cttgtcaaaa cgtggatggt ttgcaagtta atcatctttt gcgtgcgttc
     3421 agcggacact ttagctagtc cagcggctgt ttgagcagtc ttggcagtct tagcggtttc
     3481 ttgtgagatt ttttcaaggt cgtctacttt ttgcacggct tcgcccattg caatttcgac
     3541 gtaagcagtc tttttaaact cctctaaagc agcctcgata atttctgcat cattagctgt
     3601 tctcaagtct tttttgacct tctctgagat gactgcacct ctgtcatctg taataattac
     3661 ctgtgtatca acgactgctc ctgtaccgtc ccactgtggg tagtttcctg ttactttcca
     3721 atttctcatg tttatttacc ttcttccttc ggtgctgttg cttcatccaa ttgtgcgttt
     3781 aattcttcca attcgatttc atgctgtgct tttaattgag cattttccag cgttaaatta
     3841 gcgactttaa gtgttaaatc gttaataatt tttcctaata atttttcttg cattgtttct
     3901 cctaaattcc agtcatataa ttgctatgct cgttcatgat agcgctagta aaattcgggt
     3961 gtgtagtatc ccagccaaca ttttgtagat gtttccagca ccttgccaaa gctacaatcg
     4021 cataatacat tttattgata tccacaagtg tgtttacacc cgttggtgtc attttaaatc
     4081 cacggtttat accaaagtca tccgcaaaaa tgatggtatc tccataaatt tctgcctgat
     4141 caactgatgc tgtatgttca tatccgctag ccgtccgaaa aaaccttgct ccacaaaaac
     4201 gaccagatga cgcgctattg ataccatcac cagatgaagt gattccgatt gacgcataaa
     4261 gagccgacct tgtaaagttt tttggtgtcg cattaccaaa atgcacaaaa gcagtgtggg
     4321 tacctgattt tcgaacaagc gcattattgt tgctgttgaa attgatggat gcatcgctat
     4381 taaaatcgat ggtagagttg tttaaatcaa tcatcatagc accatttctt gctctgatga
     4441 ctttaccctc aagcaaactt gtaatcgtat gctcaatcct tgctttaata aagttagcat
     4501 ctaaaccaac aatgctgcta gcgttgagat taatcacatt gattttgttt gcatcgattg
     4561 tcccaccgat aatctggtcg gcttttagct tgataaactc accaagtttt gcgccaaacg
     4621 ctccattaac cgtggtattg ccgtctaagg cgatacgctc gccagagatg cggacaccgt
     4681 agccgtttaa attaatggct gagacaatct cacttgcgga cattttgctt tgcgggagtt
     4741 tgcccttaat cgcaagcata atactgtcgc ctgccccacg aatataactc tccatttcac
     4801 cacgagtgac tttttgttca atcaagccag ctagttgtgt gacacgggat tctaaccctc
     4861 ggttaggggc gctgacagtt gattgtaacc ctctaactgt ctgggttaag ctactgtaat
     4921 tcttctcagt atcctgcaat cgccgctgat agctagctag gtcctgttgc acacgactaa
     4981 cagcaccctc acgattttta atctcttgtg agatttggta ggcagttgat tgttgcgttg
     5041 attgcaaccc actgattctt gactctagca ctgtccgcat gccttgatgg ctacgagtaa
     5101 actcagctcg taagtccgca agcttactct cgtaggcctc tgtggtgccg ctagatgtcg
     5161 tggttatctt agcagataac tgctgtatct catcatcata cttttgcgac aaaccttgag
     5221 cggttgcttg tatcacagct tttaggtcgt ttttatcatt ggccattgtg gcctttaggc
     5281 cgttaatgcc agcttgataa gttgatgata gctgtctatc agcgtcttga tagtcacgtc
     5341 taatacctga gactgtctca ttaataagcg caagtttttt atcagtatcc tcactaatcc
     5401 gtgttgctat accatttgca ctttcgacaa tctctgttgt gatgttatca cgatgatact
     5461 cacgcaacat gccgtttgtt gtgagtttga tttttgccca aagctgagaa ttaacggtat
     5521 ctgtcagctc taaattaagc tcttttaact gactgagcgc tccttccaag tttttaaaaa
     5581 gtccggttga ctcaccgctg ccctcaacaa ccactggcgc cacgtagtta gttggcttac
     5641 cccctcgctc aatcatgagc tggttaaagc gagtcgtacc gatacatttg ttattagcta
     5701 atttaacgct ttttgtgtca ccatcagctt taaatgtata ataagcacgt ccgtcagagc
     5761 caatcgttaa atttgactcg tctattaaaa gtgttggatc tctactcaat tgttacctcc
     5821 taatgttgcg aaattttaaa tgttgggttt ttatacttgt ctatcccatc aaacggataa
     5881 gtccacgtct cgatagacag cattgcgcct ccgccggcaa ctgtccgaac tggtttttgt
     5941 agcttagcaa cgattttgcc gtcaacctca acagcgactt tatcaactcg atgccaaaca
     6001 accccataag tcgcaaaatc ctgattgtaa tcaatgacag cataatcatt taatttagat
     6061 acgttagcgt ctacatacgc tgtaatgttg taaacttcct gaggctctgc tgattgagac
     6121 caaatgacct gattattaca aataacgcga gctattggca gtccatcaat agtaatcgat
     6181 tggtacaaac ccatcgcggc agaaggcttt tggacgctag tatctggttt tgataatgac
     6241 tgatagttat acttattagc tttatacaca tcagcctgtg tgctagatat aattttatat
     6301 acatcaacgt cttgatctgt gtaaaagcta tagtaatcat ctatcttaat ataaaattgg
     6361 tcgacaacca aactggcttt ttgtaataaa gtaccatcaa tgctttgact gtctaagcgg
     6421 taaaaatacg tgtctggtat ctcttgcact gccattactt acctccagag cgttcaaaaa
     6481 caattaatcc gcttgtgtta tatggcactt tacctttatc aaccacacgg acagataggt
     6541 actgacctgt tgccacatct tttagctttc cgatgttgtt taactgatac tggatatcag
     6601 ctaatctaac atagccgctt agctgcgcat ttcggacaac gtccctaata tctgattgct
     6661 taacaatgtc ttgtggcaga tcagtttgtt taaggtattc atctatacca tcaatatcag
     6721 atgttctgtg ccggtgatat gtacttgctt tgttttgtag ctctatttta gtcgcatacc
     6781 ggtctaggtt tagatcagta atatcactta gcgagtgctt gtgacttgct ttagcataag
     6841 tgctgtttgc ctctgcttta gacagatagt ctgcaagctg cttactcaca tcaatctctt
     6901 taagttctgc ctttgtagcg taggcagata ggtctacgtc cttagcttta atttgggcga
     6961 tttggtcaga agtaaggtct tcaaacttta aggaatcacc cttatcacct ttttgacctt
     7021 tgatgttgcc tactaacttt tcagattctt cttcaaactt gacaaaaagg ttcccctctt
     7081 catcaactcg agcactctct actccttttc ctctatcccc tttttggcca ttagggatac
     7141 taaaggtttt ttgagtgtca tctgatagtg tgatagtaag cgtctcaccc actttagtga
     7201 tgtcggcaat acttgtccct ttatcacctt tttgaccttt gatgttgcca agcttagtct
     7261 ctttgtcgcc aatccacaca gacaagtcac cattttcgga tagctgtact ttagtgatag
     7321 attctccttg aggaccttcg tagtaaggca agtcgttgta attcaatttg ccatctccga
     7381 cctttagcct gtgagtgtct agctcaacaa ctagttcacc atcatcaaca acaggattta
     7441 atttttgcca ttcggcggca tccttgcggt caaataagac tttaattggt attctttctt
     7501 ctagcgtcat ctgtccctcc ttccgtctaa aataattttt ggattgcttg attgtttagc
     7561 tgtgattgtt gcacctttag catcaaccac agctttataa gccatatcca gagctaaaat
     7621 ttgcccctct gacgcggtta aatctatttg cttagattta taccaatcac ctgttaaaac
     7681 acctttatag ctcaaaggat agacctctat agactgttta tccttagtga cctcaaacgt
     7741 ctgtggctcc atcttagcct ttgtcggtgt cagcaccagc ttgagacctt tattattagc
     7801 ctgtatcagc gtaattgcca ctttatttag tggctcacat gtctggctaa agctaatcgt
     7861 ataggtctcg ccacgcttga aaccaccatc attggcttct acctcaataa aatcctcatc
     7921 aattttttta acacgattag ggttgccgac cagcaaattt ttgttgtagc gggtcttacc
     7981 gtctgtgcct atgatttcgg cgtttagacg ggatgtctcg ctcgtttcac tgactttgtt
     8041 tttgaggtcg tcaaagcttt gtttaatcga tgggatgtca tcaaccttga tagcatctgt
     8101 gattttttta atggcttctt ctggtaacgc taggtttttg agtgtttctc taaactcttc
     8161 aagctctttg tcagtgcgcc ggttgatttc ttcttgcgct tttttggctt tttcgattgc
     8221 ctcagagatt tgtttttcga ggtcttcacg ccatcgttct gggtcgttca caacctcggg
     8281 ttcctcccac tcagcaccat tccaaaagta cattctagta gtatcaccaa cttttagaaa
     8341 taatatgtct ccttttttta aggttccttt tggttcatct ttgggcattt ctgtgccacc
     8401 gtaaacagta tttttcccat cagcagatac tagtgcttgt gttgctactt caattgcaga
     8461 agcaatatct gcatctatct gattgattct actgtttata gacagacttc ctacgctttg
     8521 ttttttcaca gatccgatat cattacaagt gacttcatgt tttactaaag ctccagttgc
     8581 gtcgtagtag cttgtaaaag aaactatccg tatacgctct tgaaaaccta aagtctcatt
     8641 tatcgccata atgtagtcac cagcaaccgg cctctcacat ctataaccag ctctcattaa
     8701 atcttccata tcaattttta ctgatatctt ataagaattt tcaacattac tttttaaagc
     8761 ttcttttaga ttatctgcca ccgtgaagcg ctcatccgtc agaggcgccc cttcaagtcg
     8821 accgtactcc ttggcaagag ggctttcgta ttccgcctca agtctcccct tagagtggtc
     8881 ctctgggtcg gtccatgcac caaagccttt ctgataggta ataaagctat caatattttt
     8941 ttcaatggta agatcattca tgttgaaatt ttttcgtacc actgttgaca gatcagtacc
     9001 tatgtttttt aatagccgaa caactttccc gctgactgaa aactcaagtc cggtcgcttt
     9061 aataatctca ttgaataaat ctaacctaga cttataatta aaagattgtt ttctaaatgc
     9121 gttcacacct acttctagct tatagcggta cccactacct ttaaatactg tctctagata
     9181 ggtatcaatg gtatgagacc catctccgag accctcatag atactactct tgctgaagtc
     9241 ccaaaaaaat tggtgtatgg catcaaacga aacttgcgtt ttttgaccaa catcaacagg
     9301 ctttgcgtaa ataacaacgt aatactcatc ttcaaatcta agtcgccacc ctctatcgat
     9361 attattgagg acatcactgt ttgtataaat ttccccgctc actgagcgct caccattaac
     9421 agcattagta tgcctaatga cactaatggc cccatactca atgtcacgat gatccaaaaa
     9481 agtaatcatt agaccctcct atttatacaa ctctacaaaa tttaaaatcc taatcgtgcc
     9541 aagaaaatcc gttttgtaag taattctctt gttaggatta ggattgaaga taaaatactc
     9601 atagttcgtt ttagcattga cataatttcc gtttagtgtg gtagctatgc ctgtcaaaag
     9661 taatttatca ctattttgta gctggctatt ctgcttaaac tcaaagcgcc tgccatcaat
     9721 ttcaatataa aaattagtct gacctccaga gggagtaagc tctataataa aaggccactc
     9781 taactgactt gctttagcgg ttcctccata ggcaaaactg ccccctgata gagttatgtc
     9841 ctttggtttt gtctcaccga aaggtaattc agcagttctt agtgtgaagg aaagattata
     9901 ttttagtcct tgactggttt ttcctataaa ttcataagcg atagtgtcgt ctaggatgac
     9961 tttaaatcta tatttccaag gtttatgagg agagccaata agttgcaggt ctcctgtgct
    10021 ttcccctgga agctcaaagt taaatagatc tgagttttca gggtacatct tagtcacgta
    10081 gaaaggttcg tcaacaacaa gcagagcgtt tagttcgtcc tgtttgtcca aaactccttc
    10141 tacatcgctt accgtcactc ttcctttgac cttaagcgtt ttagtcgtaa aagtagcccc
    10201 atcgtagatg taaccactcc ttcctttgac tgctcggtgt gatacagata gtagaggagc
    10261 actatcttca atagcaatat tataaatacc aaagtcagac aatctagttg cctgactctc
    10321 tttttctatt aataaatcca aaatactccc ttctaggcaa aaacaaaata agaatcttta
    10381 gattgctctc ttgattcttt ttctttaaca gccgtgtaaa ttttatctcc aacaatctca
    10441 ttatgcactt caaagatagg ctctgacaat tgagactgcc taacctcttc gctaagatta
    10501 tcaagagagg tattgattcc actattatta actttagcag atgttgaaac agtactattt
    10561 accccccagt tttgatcagt aactgctgtt gcgtatgctt tacctagact attgatctca
    10621 cctatccact ctgccatacc gagggcaaac ccctctccag taaatgctcc tagtgtttta
    10681 gtgacacgag atggcgagtg aatatccaga gcgcgtctaa ttgttgctgc tacgttagcg
    10741 gcaatgctgt tagctattgc gtagattgtg cctgcgctac tagctagacc attagcaaaa
    10801 ccttgacctg cataagctcc tgcagaagac atctgccctg ccaaagccct gaatattgag
    10861 aggatcttct gacaagcatt attagacacc gcggttgcac gagacatccc agaagtcact
    10921 gaacttacaa ggtttgccat agattgcttt gcagtactgt taatgctact aaatcctgtt
    10981 ttaaccgcat tggctatccc attcattgag gttgttgctg aagtcttcgc cttattgaag
    11041 ccagactcaa ccgccgagga caacctattg gcgcttgagg ttgcagaggt tgctgcagaa
    11101 gacatttcac ttgtcacgct attagacaga ccagaggctg ctttagttga gtttgactgc
    11161 attgatgcca gactgttact cactcctgag ctcatagctt gcgcctgaga agttgcactt
    11221 gtgctagcgg ttcccatgtt tgcaccaaca ccactagcta agctgagcgt atgattaatg
    11281 gcgtctagat tcattgaaga agtcgcacca ttaacccctg aagccatttc ttgagcctga
    11341 gtagtagcgt taatattagc cacagacata ttagcgctaa tgccactcgc aagagacatt
    11401 gaatcattca acccctgaag actcatcgtt cctgtctgag cattaacatt attagccatc
    11461 tgtgtagcat ttgatgtagc gtttatatta gcaagctctg tattttgact aatgctatta
    11521 agcatagatg tcgtatctgt attggcttga ttagccatag ctgttgtttg agtaccaaca
    11581 ttcaaggcca tggccgtggc gtccgcagag actttagctg tacttgtgga actcttacct
    11641 gtgatttgat cccacatcga actgaatccg ctcttaatac cttcccaaac tcctgaaaga
    11701 gcatttggga tagcctctaa catggcttta ccgagtcccg taattaattg aatacccgca
    11761 gaaataatct gtggaagtcc ctttatcaag gctacggcta gttgtacaac aagctgaatg
    11821 cccgctgcga taatctgagg cagagcttgc gtcagtccag aaactaaaga ctgtataatc
    11881 tgtacagcag attgtacaat ctggggtaga ttttgaataa tcccctgcac aaggctaata
    11941 atgatttgaa tgccaccttg aagtaactgc ggtaaaaact gtacaattcc agatacaaaa
    12001 ctcgtaataa cttgagtagc tatctgcaga acagttggca acatttgagt aattccctgt
    12061 acaagattca ccaaaatagc tataccgtgg ctgataatac taggaaggtt agcctgtaaa
    12121 ctgttcccaa aactaccaat gatttgctgt gcttgctgga taataagagg tatattttga
    12181 actatgccgt tgacaacatt taataatagc tccattccaa cagaaataag ctgcggcaag
    12241 gcactcgcaa ttgagctaac aaaactacca atgattttta ttgctgaact aattaaactc
    12301 gttgcatttt gtccaatacc ttggacgaga ctggtaatta gctgaacgcc agcttgaatt
    12361 ataaccggta ataacactgt gatagcattt gcaaatttag caatcaattg agtaccacta
    12421 gctatcaaag ctggtatttt agaagtaatc ccttttacaa ggccttgaat aatgctaggg
    12481 ccttttgtta cagcagtgtt aagtagctgg tcgatttgtg cgccaaactg gctattaatc
    12541 aaaccaagtc ctgccaccac aagtcctagg atagcggccg gtcctatggc cgccagtgct
    12601 aaactcacaa gactggacat tgcgcttgtc atagttccaa gcacagatag cccacgccct
    12661 gctgcggtag caaaaacgcc aggtaatccg cttagagttg ccgcaaaggc accaacaacg
    12721 ccgctggcac tagctatccc actagaaatt atgccgccaa acaccccgac agaagcccct
    12781 aagcttccta aagctagacc aacttttcca aatagagaaa gcaaaggcat tatcgttgct
    12841 atagcgctag cggggttcat cactgcccaa agggcaatga ccttaggggc taaagaagtg
    12901 atttttgctt ttagattatc aaaagcctgc cctgataatt caccagtatg tgcaaaatct
    12961 ccaagaacag tattaataag agcaaacacc tctttaacgc tggcgcctaa tgggctaaag
    13021 tcaatattga tagactgttt tagcctagcg aatgctgcaa ctgcatttgg aataagagaa
    13081 ttaatcgtat caaaaacgct ggttattgcg ggcttcaggc taagaagtgt ttcagcaatg
    13141 gtcttcatac cattagcttt agccgcttca tcaaaggctg aaatcatacc tgctacgcct
    13201 tttacaacgg cggtatgtac gttcttccac gcagttcgga ttccaccagc cgatgattgg
    13261 gccatctctg caaatccgcc ttgggcatcg ttaagctcaa tcattttatc tgcgaactct
    13321 tgtgcagtga ttttgccatc agataaggct gattttaaat cttgtacgcc atttttacca
    13381 aaaccgaagg cttccgccat tttagacatt aaacctggcg cagcttctga tacagagtta
    13441 aactcttcag cttgaatttt tccagacccc aacgactggt taaactgcct gagtgcttgc
    13501 tcagcccctt cagttgttgc cccgtaaccg atcattgcgt tattgaaggc gagggctaaa
    13561 ctagtccctt tatctaaact tcctgtggtt atggcgagct gttgagcgct ttttacagca
    13621 ctgtctagag gagttggcag accttcaatt ccttttgata gcttatcaat tgatgcctta
    13681 gattgttcag cagaataccc aaatagcgcc atcgttttcg gaaagcggtt catggtatca
    13741 acgcgactaa ccgccccgtc tattgcccca gtaatagcgt gcatagcttt ttgaacaaca
    13801 gccattaaac taaaggcctg cacgatgctc atcactgtac ttttgagtct ctctccagca
    13861 gaagaggtgt tggcgaattt ctggctgatt ccattcaagg aagtagccgc agaactagac
    13921 attctgctaa aaccattgct aagactactc cctattttac ttgataaatt agctaaactt
    13981 gaggtcagcc gaccaaacaa actagtactc actttgtttg cgaaagcaga cgccttagct
    14041 gatacagaac taaaaaaatt attccatgac tgtaaggctg ggttcaagac ttgccgacca
    14101 agtgaattag aaatcttccc tccaacagac tgtatagagc tattcagggt agaaacggat
    14161 gaggcaattt ttctaaacgc tgttccgaat ggtcgcggaa gcttgcctgc gaccgtttcg
    14221 aatattgcag ataagttatt aagcttacta cttgccctct gagttaagat atcaactcca
    14281 gtcgcagcct tggcaaatgc agatcttatc ttaccaatat ccacaagatt ctgaagttgc
    14341 atttttgttt gattaatgaa accggttaag ttaccccgta ttttgtcaat aacacttgca
    14401 actggaataa tgattttttg aaaaccattc tttatgggtt cgggaatttt ttcgccaatg
    14461 gaagctgcta tgcgttgaat ttcaccaata ctcagtttta aaccagtagc gtaggcggaa
    14521 ccaagtctct ttccgagcga ttcgccgttg ttcgctaatt gggccatgat ttgtccaatg
    14581 atttgaacca ttttattact actgttaacc gctgtatctt gggcttttct gaaagcattc
    14641 tgagtcgtac taacaatacg agccatggcc tgctcatagt cctttgtgtt ggcaccaata
    14701 tcagcgaaaa tagaaccatc aaacgttcct ggcatctact cctccttatc tatctatttt
    14761 gaaaatgatt attaagcatt tgtattctct caacaatact gctatcacta tcggcttggc
    14821 tacggctagc tttttgatac gatcttttga tagttagcct atgcttatcg tatttgagct
    14881 tacctacatc aaccttttta gcatttaatg tatatctaag ttttagagac aattcagaga
    14941 ggttctcgcg ctcagcaacc tgcttatact caagcccctc cataatagcc tctagctccc
    15001 acttagtaca tttatagata gtatcaatgt ctgtgagccc taaccgtgcg caatttacta
    15061 agatactgcg ttgctcattc ttccaatcat gtcttcgata atttggattt gagttgcgtc
    15121 cgtgtcgtcc ttcgctttga gataacgagc tgatttctcc atgttttcga tatatttcaa
    15181 aatcttcgct ctgaaaaaac cagaatcaac catttctttc tctatttcag caaacaaccc
    15241 ttctgttgtt ccttcttcat caacagcttc tgcaattgca tctagaattt catcttcagt
    15301 tagcgctttt tttccgcttc ccgcagataa acgcacaagg tcaacaatgg cgctttcatt
    15361 acgctctaag atattgaaaa acaaagcacc aacaccatct gcattgcgct ctcctgtttc
    15421 tgggttaatt gttcctagtt tattattgat cttaaacata gttttaaaat caaatttaat
    15481 atcgatattt cgtttcgctg ctgtaaattc cattatttta tctccttttt aaatcaaaat
    15541 ggggcagcag ttgccgcccc tgtgtactac ttagtgatat tatcgtagtc gccagtagta
    15601 tcacccggat tttgatagcc gtatacgttt tctagcattt caatttcttc tttcgtgaga
    15661 gggaatttac catcttgtaa ggcccctaca atgttagcgg tgtaggacat ttctactaag
    15721 tctgaaatac cagtaccgcg ttcgatttcg tcaatcttgg cgtacccaaa cttagcaggg
    15781 taaacatctt tcttgggtcc agccgattca gaaatttgga tctgttcttt gacactttcg
    15841 tcggcaatga cttcccaaat tttgatagat ttacctgttt tcttagcttc ctcgatgaca
    15901 gcaacagatg gatctgtcgg tacaaagtac gttgtcaaat caatagagtg ttcatctgta
    15961 gccttctcaa taacgcggcc ttgttgggtt tgctcatcaa tgtattcacc gcccatggtt
    16021 gtagtaccat cggtacgata ggctggcagc aaagccttgc tacctttttt agcatcaact
    16081 gattggatga aataaaaaat ctttttccct acaatcggtt tacctgttgt aatttcaata
    16141 gttgttcctt ctttagtgtt tgccatttag gtctccttta aaatagtgtt tctgtaatgt
    16201 tgatagcaac tctatagact tctcgtccta ttgaattatc taccaaaatc tgagccttta
    16261 tttggttatt ccgcccaagt agtctaagca ggcgagcttt aatctcttct gcatccaaac
    16321 gactatcgcc aggtaagaaa gcatcgatat tcaggctaaa atcgtcaata actgctccag
    16381 tttgagctgt ttttgacaag tctgatatat ttgtacctac aacaataaaa ggctctaaaa
    16441 cgtctgattt aggcaattta aaatagattg gtatgcctaa agctcccaat ctatttttta
    16501 ccttttttaa atatagtgtt tctggtgagt aatccatgca tcacctctta aacatttttt
    16561 tgagattagc cattagcaca ggccattctt ttctcagggc agggtctaaa aacggttgag
    16621 cttccatttt acgagtacct aattcaagat aaatagaata taaagcagga gaaaccactc
    16681 tatagtgtaa gagtcgttgt tgctcattgt agatttgagc tctcagccat ccagtatcaa
    16741 ccggggctag tattttagcc tgtctctcaa tcctagcagc tgatttacta agctctttat
    16801 ctacagcgat tcgtactgac ttctgcttgc gctcaacgct gcgtaaaaac ctatctaggc
    16861 ctctcatccg ataaataaga ctcatacata aataaccgtg ctatttttat ggtgtgctag
    16921 tccctttatc aacctcggtt ttcccttgta gataatcttg ctaaagccgt cgtaatggcc
    16981 ttgcaagtgc aacttaaaag cattcaaatc atatttacca aatagcccca tctgttcaga
    17041 ttttgtgaaa gtattttcca tgcaggggat tttcccgcta ggtttttcaa cagttctttt
    17101 atctagaaaa tcgtcctgct catcgacata cactaagatt actctatctt tatagatcat
    17161 tgtgtatctc ctaaataaat cttgctaccc ctttgcctga atataccttc ccaatagcag
    17221 cgcttctgag tgttgattca tactctttaa ggtaactgtc ccacttaaaa gtctttcctt
    17281 cttcgctatc ttcaacggtt ccttctgaat taatcctatt aaaccgttta atagcaacat
    17341 cacggacaat aaacctcaat ctatcaggaa ttttggtgat ttcagtttct gagtattcat
    17401 taagtttagc tagcacacgc tctttacttt cagatatagc aagggtgatg agctcatctt
    17461 gcgcaccatc atccttggaa atacctataa agagtttgat atcgtcaata agttgtttat
    17521 cgcccatata aatagccttt aaccaccaat gccgggtaca gcagcagctt cgattgttgc
    17581 ctctacaacc ccttcaggaa tttcagcaaa cagcacattt gccccaaaga aaacagattc
    17641 ataggtaaga ttagaaagct gacgattacg agctgcagca attaaacctg tctcatcagt
    17701 aaaatcagca aacaaaccac ccaagtctcc gcctttaaca tttaaagatg caaaaactag
    17761 attctctaca gccgttgagt agattttacc ctctggcaca gatggcataa caatcacgtt
    17821 ttgcataccc aaaaagttct taagcaatgt cattccaaaa acattagaag catccgcacc
    17881 tactttagta tctccaagat agttggctac gtctaaagga gagacaaaag aaacaagcgg
    17941 ggaaccctca aactcattaa aagtagctag cttagcccat gatgcagaca gcgctttttg
    18001 aaggccaacg cctttaactt ttgttggttt tgttttgagg aatgtaaaga atgcatcctt
    18061 aattccattc tgaagctcac gcataatgcg cttatctgct tcagtaatag cacgagctgc
    18121 gccatggcga gcaattgact cagctgtagt tgcacgacgt ttcttgaacc acttcactgt
    18181 atagtcttta tctttagttc gagtaacctt agacagaggg atggtttccc cttctccagg
    18241 atcagtttga tctaaagtca cttcccactt ataggtctga atttttagat cgttagttaa
    18301 tgtttcacga cgagtaaccc ctagcaattt tagtaaatca ttaatatttt tggaaaactt
    18361 gttaacaaaa tcaattgatt taatatctcc taagtccgcc atagtgttta aatttttttc
    18421 agccataata ttcctctctt aaaataagtt acgattctca gcaattaaac gcatacgttc
    18481 gtcatcatcc tctattgcca tgatgtcagc cttagtcata ccgaccgagc cgtttccagt
    18541 tcgtggtggc ttctgggtta aacgatcgtt aacacgtttt tcgacagctt tatcaaaaat
    18601 agtttgtaaa ttatcaatat tggccttaac ctcttctgca gtagtggtta aaacaatatc
    18661 aataaaatcc aatggcaatc ctttctccac aagcagggaa tgcgtttcaa tgcgcatctc
    18721 gcgctctgct actgcttttt cacgctctgc gatagcatct agacgttttt gttcctcctc
    18781 tttagcgcgc tcatcttttg acaacttggc caaacgttca ccctctgtac gagctttttc
    18841 aatagcttct gattgttcag attcccattt agtgcgttcg gcagccatca tttttgagat
    18901 gtctgcgcga gtaaaagttc gctcatgttc ctgcttagga tgttcaacag cttctttttg
    18961 agtgtcgact tgctcagttg tttctgttgt tacattctct tctgccataa ctttctccta
    19021 gtggttacgc caccaaccga tagtctagct ttacgtcctg cgacgaaaca gtgtagcttt
    19081 tagtgtcatc aacaaagttt ggacacaata aaagccgtat tgctacgact ttgatttcta
    19141 aaggggtcga attcgtttaa aatttatttt ccccacttac gcttataatt tttcttaatg
    19201 tagttaacat caattgcaat gtttgtaata gctgcttgat tatctaaagt agcagcttta
    19261 acagacgcaa actcttcgtt tgttgccaga gcgttccgct gaacaattga tttcaactcc
    19321 atgatttctt tgttttgatt tttaattgct tctgcttgca tggcattctc tgcaacaagc
    19381 atcacaacca ctgtttccaa tttacgtttt ttcttaatgc gcttattcat gtttatccta
    19441 cctttttcaa ttctgcaata attgacttaa ccgctattgc tgcaatgcca acaatcaata
    19501 tgagcaccaa tacacctagt gccactaata ccatttgcca aataaacata ttttcctcct
    19561 tgatagtttt aatagggtga tgtataattg agataaagaa ggatgatggt ctaacgccca
    19621 cgaagcagtt tactgtggag gcggtggggc atcatccttc tttttctaat acagctaata
    19681 cctcatcttt atttttgata attgatatat caagacccct acgatctatc atataaattt
    19741 gttctaactg ccccattatc tcttcaaatg ataaaggggt ttttgttata tcaaaaatga
    19801 tattagttgc ctgctttttt gcttttcgta gattgccatc aatgacgttt ttacccgaac
    19861 ctgtgatttc ttttagatca aaaggaactc tatcaactaa ataatcagga gtattgatat
    19921 gttcagggga attaactctt ggcaccatgt caacttgcaa tcctgtcttt ttagataacc
    19981 aattagcaac tctatattca taattcgagt gatcaagaac aacattatgg ccatctactt
    20041 tgtaaggagt gccatttttt acatactcat ttagtagtga cagttgtggt ttatgcccat
    20101 ttccatctat ccaatctgag gtgacatcct taaaatactt agtgctttta tcatcaattg
    20161 gttctacttc aatgcttagc tcatcttcat caggaataag ggttgttctg cagttgaaat
    20221 gataaggtgc tgcattaatt ccaggcttta gctcagataa tagctttctt ttgttttctt
    20281 tagcgatttt acgacatatc tcagttgtcc tgtcatcacg cctaactaaa tcacgataat
    20341 acttaagacc tgcatccaag tagcgcctag cggtagcatt attaatcacc atagttccat
    20401 cagttctgat tagtgtttca gccctgtgat tggcaacatt aaattgctta gctaaatctc
    20461 tggctatcgc cctaggatgc tcgccacgaa caaaaccttg ttttaatttc ttttggagat
    20521 tcttgacaag attgtcagta ttgccccaca gctgttctga atagttataa ccatcaaacg
    20581 gggttctaac cagttcttct aaaactggac cattgattgt ccctgaccgt ccacccattg
    20641 cttttttata agcatatgag gcagcttttt ttaaatgatt ttcaaatgct gaatcaagta
    20701 caccttttag tatccctgcg cgataaacaa gttcaagatt taatgcatcc atccttgtag
    20761 ctcttgctga tacatactgc tcattaagtc gttttagcag ctcagggtct ttctcagctt
    20821 gtttgcgata cttgttagca ttagctacgt aatctgataa atcttcatga cgtagctttt
    20881 taacgacatc ctgataagtc attttgttct cttcagaata cttagtataa aaatcaaaca
    20941 attccttttg taactcaaaa gcctgttcat tatataactt ctgcagttct gaaaagacat
    21001 ctaaatcagc tctgtctaaa taacttagaa tatcttcttg acgcttttgc cagtaattaa
    21061 ggcttttctg ctgttggttc ttcttgacca ctagcatcac ctaccaatct aggctccggt
    21121 aaagattgct ttttatcagc ttcttctttc aaacgttcaa gctctgcttc agcgtctact
    21181 cctgtgacag tgtttaagat ttcaaagata gtctgatcgc taacaatgcc ataaagattt
    21241 tgtgcagctg taacaatttc attatcgttt tgaggaagat taggtgtgaa aactatactc
    21301 gtatcattta caaggctata tgtagttgct tcattccctt tgatggccca gatattagct
    21361 gctaagcgta agcgccgcat taagcctttt ttgaacaacc tctcttgctt ctcgcgatag
    21421 ttgtcagaag ccataagctt atacttcatt gattcaccag attgaactcc tgaaaatttc
    21481 atatcttgtg tatctggcgt aaaagtaaac ctcaaaatgt ctgcaactag tctatttttg
    21541 taggcttcgc taccggcggt atcatactct tttttgagaa agtacgcttg tggtttaacg
    21601 ccatttggat taggattatc gtctaaaatt aacacttggg cttttttaaa cccaattgag
    21661 atcgctaaac gaccattagg atttaatcgt ccgtcatcta agtagtcatt ctcatcagcc
    21721 tctgtgtaag cattaccagc tattacaagc aacgcattga ctgaatcttg ttgaaaatta
    21781 gcaagctcgg actgagataa atcataggca tcaatattat caagtacaga ctcataagcc
    21841 cctgtacgct cctcattatt agcatattca ttgactggta cacctttaaa aaaatgttct
    21901 tcatgctctt ttaagcacgt accttttgtt tcgagattat agtcttcgta ggtgtaaatt
    21961 gtgtcggaag tatatacttt gataatctgt ttgcgtttac ctgagccata atctatgttg
    22021 taaaaatgaa cggccatcaa cgaatttcgc tggtatgtat catcatagat aacaaaagtt
    22081 tgctcggctg ggagctggta gagttttact tcggtttttt tgtcatctat ttcctcaaca
    22141 gttaacagct cgtaggctct tccataaatt gataaatctg tttttatttt gacgttgtgg
    22201 tagtcctcat tgtttctgac cgacataagg tcaatagccg cctggaggtc tttattttca
    22261 tttttgtact caacgggaac accaagcata taaccttgct caaataccgt aatatattta
    22321 gcaaaatcac ttgcaatacg attatccgct gcgtatttat ctgttttagc aggtctatac
    22381 ttgatgttat tatccccaag atagtatcgc tttaactctt tcaaacgctc taattgttct
    22441 gctttaaacc ttgaaatgta attcttaagt tgatctatcc aaagttttga ttcatagtca
    22501 attgcttcaa agtcttcttg cagcataata aattgcttat tagcattctc attaaaacga
    22561 gttcctgcta aaaattgttt ttcttccaaa gtctcacctc attcctaaaa ataatattta
    22621 gcatttttca tgcgcttact tctatccgcc gactggtata ttctgtcttg tacggcatat
    22681 cttatggcgt caatgcagtg gttataactg tcaactggtt cgttgatata ctcattagtt
    22741 ttcttgtctt tcttccaagt gtaattttcg agttcttcaa tagtcttgac acacctttca
    22801 tcaacaaccc aatcatactg aagcaggtac tgaattcctt gcataacggt tccaggccct
    22861 tttgtaacat caatcattct aggaatacct aaattcctca gctcttggtt agatttcttt
    22921 tcagccgaat ctcctctgat ttcttctttg gcatagccga ggtcctttat agcatttgct
    22981 attttgtcat tggtcaaatt ctttctgaca tattcctcta tgacgtataa cttcttgttt
    23041 gcgtcatcga ttttaacatg caaaaatgcc gaagggtcat tgataaaccc atagtccaaa
    23101 ccaaaaaaag aagacaagtg tgataacttg tctttgttta atatttgctt gtcatatttg
    23161 ggaaaaatta gtttatcaag tgtagcaaac tggccaagag catatatttt gtagtaagct
    23221 tcattcctat cggctagttc ctcaatattt tctctcgtga catcatctaa gaagcggttg
    23281 tctttataag ttgtttgata gacaactgta ttcttaggtg tcttaacgaa gaaggcttta
    23341 taaacccagt ttacttttga cacagggtta aacatcaagt atatttgctt ctctagatgt
    23401 ttcttatctc ttaaacgcaa agtcaactgc gtataatcat caagcgtaaa ctcactagct
    23461 tcttccatga cgacatcaga tatcccctta attgacttaa tcttctcagg gttatccata
    23521 cccttaaaaa tgaattccga gccattcggg agcgttattc taaaagccga catatttatc
    23581 ttacatttat ccaagatgcc gaaatacgac aaattagaca tgatatcagc aaatacagag
    23641 tctctaacgg ttgcaccgac ttttctgaga actaatatct ttctgggatg tttgaattta
    23701 gggttaagtg cttttaaaat tatcttttga aaaacaccgt gagactttcc gctagacgcc
    23761 ccaccataat gaacttccgt gaagttgcta tagttgtaaa gtttgtcgta gatatgtttg
    23821 ttaaaaactt tgctaggata cttgattacg atattaattt tagggcgctg tttaatcgtc
    23881 atcccactca cctacgttaa tggttacatc tgagctgaca tccatttcaa ccttgtcagt
    23941 gaataatctg taccgcttac ctagtagctc agctgccttg attctatctt ttgcaccaac
    24001 atcgatgtca actatcttct ggccaaactc tccaacgcta atgagcgtct tttcttgttg
    24061 gtctcctcgc ataattgaag tgagatattg taggacttct tcttgcgtag caatcttttc
    24121 agattcaagt ttttcaagcc gctcgtctat ataagcttta atgtcaggtt tggtcaagtt
    24181 ttcttgacct attgaccttg ctgtcttttt actataccct gctttaatag cagctgctgt
    24241 cgcgttagct gagatgatgt actcatctgc aaaacgcttc tgttttaggg ttaatttact
    24301 caattttcca tcacctccaa gcataacaaa aaggcaagac actatttgcc ttacctttaa
    24361 actcatacta tcaatttatc atcaaaaaga tgacaattcc atacattttt gtgtcacatt
    24421 cctatttttt tggagaatat ttctagaatc cgctctcttt tgcggtaaat agacttacgt
    24481 gacagatgtc ttgtataggc aatttcttcc caagtgttac ttgaaccaac accccaccta
    24541 agattaaaaa tatcagttag ctcttcatcc aaaagattta gcgttgcaat aactgcttct
    24601 ttgaaattgg atagaccctt tagttcccta tctgaatccc accttgcaac cacatcctca
    24661 gtaacttttg aaacaaaatt tgctcttcct ccaccgatat ttttatctac ttctgtattc
    24721 atatcagttt gtagctcaag ttttcgaagt gcaatcttgt tatcaataaa acgataatca
    24781 aacaaccatt catcaaaagc ctttagctgt gcattagata atctactcat cccgttacct
    24841 ctttatgaat aattacgtga gaaatatttt gtttgggtat aataactgcg ttgttttttc
    24901 catttagagc aatgacatcg ttatttttca ttgcgccaat tacttcatca atacaaattt
    24961 caccataatt agaaacgctc ttagtaggac cttgctttaa aaaaaattca attttcattt
    25021 gctacctcca taagattttc accgcaaaaa ggacagtgtt tctgactata tgtttcattt
    25081 ttacactctg gacaacatgg tactgcataa gttgttaaat cacctacagc atctatcccg
    25141 ttttccattc tatggattat attcatacct acccccccat ttcctgtcaa ttctgcaatc
    25201 cgtttgtttg cgatattaaa gtaatcttta tttagctcat atccaataaa gtctctcccc
    25261 gtcagtagac aagctactcc tgttgatccg ctgcccataa aaggatctag gacaacagcc
    25321 ccttttgttg tgtgtcgcgt aatgatgtcc ttcatcagag ctactggctt ttgtgtcgga
    25381 tgctgtgttt tttctgctcc tgccacaact ggatatctgt attctggcct atcgtatttt
    25441 tcagatttac gattaaatac ccacttggat ttttttcgga cgacccatac cccgtactcg
    25501 ctatctgtaa tataccttct atctctgttt ctgggcatag ggttatcttt tacccaccta
    25561 aaaatatcct ttactactaa cccatttttt tcacaatgtc ttgcaatatc tcctaaattt
    25621 ttccaatcgt tgaagattat catgccaccg tttttagtta atttttgact tgcaatgtca
    25681 atccaactca gtaaatcaaa acctttatcc caatcaccaa aatctatacc tgttcgcccc
    25741 atggtagcaa agttattttt tactgatatg ttgtatggtg ggtccgttat caccatgtcg
    25801 atcaatccat cagttaactc cgctagtaat tctaagcaat ttccgcattt tatttccatg
    25861 tgttttctta ccccccattc cctgtcaatt ccgcaatccg ttttgtctgt ctagctctat
    25921 cctcactcgc acgtttaagc tgcttttgtg tcctgcttag ctgtgtccgt aatcctgtaa
    25981 tttgcggctc atagtggttt tgtaaggcta tacttatcat cagtaacata ataactgctg
    26041 tcattaagac ggcaattaca ttagactgca tatacaatct atcatctttc cgctttatct
    26101 cttctagcag cggttttatt agttcttctg tcattttttc cactccctag tatatttttc
    26161 tttaactgtt gcttcgcaag aattccaaaa cttcaacatg tccgcttttc gcgtaaaggt
    26221 tttctcttca aactgaggtt ttgcctcaaa attcttttct ctacccgtgt agattctaac
    26281 gacatattct tttttcatct agactcctaa ctgcgcctta actgcttcga gtaaggcgtt
    26341 ctgatttttt tctttgcctt gtaaaatcct aagtactttt tcatcaactg tattttctgc
    26401 aacaatgtgg tgcacaataa cgggttctgt ctgcccttgt ctatctaatc tggcattagc
    26461 ttgctgataa tattcaagac tccacgttag cccaaaccaa acaataatat gcccgccttt
    26521 ttgtagatta agcccatgcc cagctgattg agggtgacac agaagaattg gtattttccc
    26581 ggaattccac ttgtcaactg acgtcaactc ttcagcctga gggaatcgtt tcttaagtct
    26641 ttcaaaatca tgttgatact gataaaaaac taagattggc tggccttggc tttcttctac
    26701 tatgtcctca agcgcgtcaa gtttgtcatc gtgtatagca actgttgatt tatcatcatc
    26761 atagatagca ccattagcca tttgaagtaa tttattggcc aagaccgcag agttagccgc
    26821 agatatttcc ttatccttaa actccaacac caaatcagct tcaagctgtt tgtaagcttt
    26881 catattagat aacttaactg atacaacgtt gttggttcgc ggcggtaact tgagatagtc
    26941 tttagctttc atgctgacac agatgtcctc aatcttgtta tagatttctg cttctgcgcc
    27001 atccctaagc gcccaactgt aaatgattgg accattacgc ttatcaggaa caaaatactt
    27061 gtctttaaat cgagtctggc tagtctcaag cctgtcgcct ctgtccatca gataaatctg
    27121 cggccacaaa tcaatcaaac tgttaggcgc tggggttcct gttagtccta caaggcgttg
    27181 gactttcggt ctaacttttc gcaaagccct aaaccgtttt gacttactag acttaaagct
    27241 tgacagctca tcaataacca caaaggtaaa cggccattta gtcttgtagt attcaacaag
    27301 ccaggtaaca ttctcacgat taatcaaata gatatcagct tctgtttcta acgcctcaat
    27361 tcgttttccc tcgctcccca aaactttaga gtaggtgaaa tcaaaatgcc atttctcaat
    27421 ctccgtaggc cacgtttctt ccgccacttt tttaggggct acgattaaaa tcttatgatc
    27481 ctcggaaaaa atattttgaa tctcatctat cgccgatagt gttgtcagcg ttttaccaag
    27541 gcccatgtca agtaaaaggc cgcaataagg gtgctctact atccatgtct tagcgtattc
    27601 ctgatactcg tgcagtctca cacccagttc tccatttctt ttaaggccat gtccactgat
    27661 tcgtaggagt caacaaccca aacatgctgc ccagcctctt ttattttttt gtgcattgca
    27721 acttggctgg gtcttggttt tttaccaggc gctttgacct ctacaaaaaa ggttcccgta
    27781 ttcatgacaa caattctgtc aggcactcct atcgtccctg gactagtaaa ttttaaacac
    27841 agcccttttg ttttcttttt caaataattt tcaatatctt tttcagtcct catccttcct
    27901 cctttggtca ataaaggtca gggttaccgt ttttttcaac ttactttctc ttttttat
//
LOCUS       DAXWIQ010000022        26455 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000022 DAXWIQ010000000
VERSION     DAXWIQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26455
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.022"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(171..1124)
                     /gene="manA"
                     /locus_tag="ACGEUI_000969"
     CDS             complement(171..1124)
                     /gene="manA"
                     /locus_tag="ACGEUI_000969"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFF8165813.1"
                     /translation="MSEPLFLKSTMHDRIWGGTKLRDVFAYNIPSDTTGEYWAISAHP
                     NGVSTVTNGRYQGQPLNTLYAQEPALFGNPKEEVFPLLTKILDANDWLSVQVHPDDAY
                     GREHEGELGKTECWYIISAEEGSEIVYGHQAKSKEDLRAMIEAGAWDDLLTRVPVKAG
                     DFFYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRKDVNGNLRDLHIEKSIDVLTI
                     GKPENSVPATMVLDNMVATTLVSTPFFTVYKWVTSQMVDMKQAAPYLLVSVLKGQGKL
                     YVDQKAYELEKGMHFILPNDVKSWSFDGQLEMIVSHPNQLV"
     gene            complement(1219..2103)
                     /gene="scrK"
                     /locus_tag="ACGEUI_000970"
     CDS             complement(1219..2103)
                     /gene="scrK"
                     /locus_tag="ACGEUI_000970"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFF8165814.1"
                     /translation="MGKLYGSIEAGGTKFVCAVGDEEFTVVDKTQFPTTTPEETIART
                     IAYFKAFEADLAGMAIGSFGPIDIDPSSETYGYITTTPKSGWANVDLLGQLSAAFKIP
                     FDVTTDVNSSAYGEVLARPGVESLVYYTIGTGIGAGAIQHGHFIGGLGHTEAGHTYVM
                     PHPDDMAKGFLGVCPFHKGCLEGMAAGPSIEARTGVRGERLDQEADVWDIQAFYIAQA
                     ALQATMLYRPQVIVFGGGVMAQEHMVLRVHDKFTALLSGYLPVPDLTDYIVTPAVADN
                     GSATLGNFALAKLAAQGK"
     gene            complement(2367..5354)
                     /locus_tag="ACGEUI_000971"
     CDS             complement(2367..5354)
                     /locus_tag="ACGEUI_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase family
                     protein"
                     /protein_id="HFF8165815.1"
                     /translation="MDKHLLVKRTLGCVCAATLMGAALATHHDSLNTVKAEEKTVQVQ
                     KGLPSIDSLHYLSENSKKEFKEELSKAGQESQKVKEILAKAQQADKQAQELAKMKIPE
                     KIPMKPLHGPLYGGYFRTWHDKTSDPTEKDKVNSMGELPKEVDLAFIFHDWTKDYSLF
                     WKELATKHVPKLNKQGTRVIRTIPWRFLAGGDNSGIAEDTSKYPNTPEGNKALAKAIV
                     DEYVYKYNLDGLDVDVEHDSIPKVDKKEDTAGVERSIQVFEEIGKLIGPKGVDKSRLF
                     IMDSTYMADKNPLIERGAPYINLLLVQVYGSQGEKGGWEPVSNRPEKTMEERWQGYSK
                     YIRPEQYMIGFSFYEENAQEGNLWYDINSRKDEDKANGINTDITGTRAERYARWQPKT
                     GGVKGGIFSYAIDRDGVAHQPKKYAKQKEFKDATDNIFHSDYSVSKALKTVMLKDKSY
                     DLIDEKDFPDKALREAVMAQVGTRKGDLERFNGTLRLDNPAIQSLEGLNKFKKLAQLD
                     LIGLSRITKLDRSVLPANMKPGKDTLETVLETYKKDNKEEPATIPPVSLKVSGLTGLK
                     ELDLSGFDRETLAGLDAATLTSLEKVDISGNKLDLAPGTENRQIFDTMLSTISNHVGS
                     NEQTVKFDKQKPTGHYPDTYGKTSLRLPVANEKVDLQSQLLFGTVTNQGTLINSEADY
                     KAYQNHKIAGRSFVDSNYHYNNFKVSYENYTVKVTDSTLGTTTDKTLATDKEETYKVD
                     FFSPADKTKAVHTAKVIVGDEKTMMVNLAEGATVIGGSADPVNARKVFDGQLGSETDN
                     ISLGWDSKQSIIFKLKEDGLIKHWRFFNDSARNPETTNKPIQEASLQIFNIKDYNLDN
                     LLENPNKFDDEKYWITVDTYSAQGERATAFSNTLNNITSKYWRVVFDTKGDRYSSPVV
                     PELQILGYPLPNADTIMKTVTTAKELSQQKDKFSQKMLDELKIKEMALETSLNSKIFD
                     VTAINANAGVLKDCIEKRQLLKK"
     gene            complement(5585..7468)
                     /locus_tag="ACGEUI_000972"
     CDS             complement(5585..7468)
                     /locus_tag="ACGEUI_000972"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFF8165816.1"
                     /translation="MDNRQIAAEVIEALGGRENVRSVAHCATRLRVMVYDEGKIDKEK
                     AEAIDKVKGAFFNSGQYQMIFGTGTVNNIYDEVVALGLPTSSTSEQKAEAGKHGNIFQ
                     RAIRTFGDVFVPIIPAIVATGLFMGVRGLVTQPAIMDLFGVHEYGENFLMYTRILTDT
                     AFVYLPALVAWSAFRVFGGNPIIGIVLGLMLVSNELPNAWVVASGGDVKPLTFFGFVP
                     VVGYQGTVLPAFFVGLVGAKLEKWLHKKVPEALDLLVTPFLTFAIMSTLGLFVIGPVF
                     HSLENLVLAGTQAVLHLPFGIAGLIVGGIQQLIVVTGIHHIFNFLEAQLIANTGKDPF
                     NAYLTAATAAQAGATLAVAVKTKSTKLKGLAFPSTLSALLGITEPAIFGVNLRYPKVF
                     VSGLIGGALGGWVAGLFGIAGTGFGITVLPGTLLYLNGQLLQYLVTMLVGLGVAFAIA
                     YTWGYQDRETLPLPAVEVDQTADQPALAEETLYSPLNGTVVDLSAVSDPVFSSGAMGQ
                     GLAIKPEDNTLYSPVDGKVEIVFETGHAYAITSSQGAEVLLHIGIDTVSMAGDGFESL
                     VAVGQAVKKGDLLGHFDPSKIAEAGLDDTTMMIVTNSADYQSVDILAQGHVLIGDQVA
                     LIK"
     gene            7710..9149
                     /locus_tag="ACGEUI_000973"
     CDS             7710..9149
                     /locus_tag="ACGEUI_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFF8165817.1"
                     /translation="MDLPQAIRYRPYKEWSSKDYQAITEKMAQSPWHSQFHVEPKTGL
                     LNDPNGFSYFNGRYHLFYQNWPYGAAHGLKQWVHMTSTDLVHFTETRSRLLPDHAHDS
                     HGAYSGSAYAIDDKLFLFYTGNVRDANWVRTPLQVGAWMDKQGNISKIPQVLIEQPDD
                     VTEHFRDPQLFSYQGQFYAIIGAQGLDGKGKIKLYKAVDNHVDNWRFIADLDFDDSGT
                     EYMIECPNLVFVDDKPVLIFSPQGLAKADLDYQNIYPNTYKIFESFNPETGQLLGGGA
                     LQNLDFGFEAYATQAFSSPDGRVLAVSWIGLPDIDYPTDRYDYQGALSLVKELRIKDG
                     ILYQTPVSALQNLRGPAELFHNKIDSSNCYELELTIPGQKKLDLLLFADQKGNGLRLK
                     VDTTKGQLSIDRSRAGVQYAQDYGTVRSCQIPQGHVTLNVYVDNSILEIFINQGQKVL
                     TSRVFPTHGQTGIQVVEGQAFGHYYEMRY"
     gene            9154..10119
                     /locus_tag="ACGEUI_000974"
     CDS             9154..10119
                     /locus_tag="ACGEUI_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8165818.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVNKVNKAMREL
                     GYKPNNLARSLQGKSTQLIGLIFPNISNIFYAELIEHLEIELFKQGYKTIICNSEHNP
                     VKEREYLEMLAANQVDGIISSSHNLGIEDYERVEAPIVAFDRNLAPNIPVISSDNFEG
                     GKLAAQTLQKHGCQNIVMITGNDNSDSPTGLRQLGFNYQLKRSAEIIKLPNNLSPVRR
                     EMEIKSILATRKPDGLFVSDDLTAILIMKVAKQLHITIPEDMKVIGYDGTTFIQQYVP
                     QLATIRQPIDEIAKLSVEILIKKIKKEKTSKDYILPITLLPGASI"
     gene            complement(10260..10712)
                     /gene="nusB"
                     /locus_tag="ACGEUI_000975"
     CDS             complement(10260..10712)
                     /gene="nusB"
                     /locus_tag="ACGEUI_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFF8165819.1"
                     /translation="MTNSFQNSRRDLRERAFQALFNIEMGAELLAASQFAYGYDKVTG
                     EDAQVLELPIFLLSLVTGVNNHKEELDNLISTHLKKGWSLERLTLTDKTLLRLGLFEI
                     KYFDETPDRVALNEIIEVAKKYSDETSAKFINGLLSQYVSGAPSANKS"
     gene            complement(10705..11094)
                     /locus_tag="ACGEUI_000976"
     CDS             complement(10705..11094)
                     /locus_tag="ACGEUI_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8165820.1"
                     /translation="MTTEYIGEIVISPRVLEVITGIATTQVEGVHSLHNKKMADSFNK
                     ASLGKGVYLQTEEDGSVTADIYVYLQYGVKVPTVSMNIQKTVKSAVYDMAEVPISAVN
                     IHVEGIVAEKTPKPDLKSLFDEDFLDD"
     gene            complement(11140..11697)
                     /gene="efp"
                     /locus_tag="ACGEUI_000977"
     CDS             complement(11140..11697)
                     /gene="efp"
                     /locus_tag="ACGEUI_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFF8165821.1"
                     /translation="MIEASKLKAGMTFEAEGKLIRVLEASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYKMDDTAYFMNTDTYDQYEIPVANVEQEL
                     LYILENSDVKIQFYGSEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATLETGLVV
                     NVPDFIEAGQKLIINTAEGTYVSRA"
     gene            complement(11793..12254)
                     /locus_tag="ACGEUI_000978"
     CDS             complement(11793..12254)
                     /locus_tag="ACGEUI_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HFF8165822.1"
                     /translation="MTNRLSWQDYFMANAELISKRSTCDRAFVGAVLVKDNRIIATGY
                     NGGVSATDNCNEAGHYMEDGHCIRTVHAEMNALIQCAKEGISTDGTEIYVTHFPCINC
                     TKALLQAGITKITYKAHYRPHPFAIELMEKKGVAYVQHDVPQIVLGGDKEQ"
     gene            complement(12289..13362)
                     /locus_tag="ACGEUI_000979"
     CDS             complement(12289..13362)
                     /locus_tag="ACGEUI_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFF8165823.1"
                     /translation="MSGFLEQRLGHCLRQMAEKGLEALLVTHLTNSYYLTGFSGTAAT
                     VLITAKRRVLITDSRYTLLAKASVEGFDIIESRTPLKVVAELLEADQIDCLGFEDQVS
                     FSFYQAMQAELSGITLLAQSGFVEHLRLIKDASEIDTIAKACSISDKAFEDALDFIKP
                     GTTTERDLANFLDFRMRQYGASGTSFDIIVASGYLSAMPHGRASDKVIQNKESLTMDF
                     GCYYNHYVSDMTRTIHIGQVTDEEREIYALVLAANKALIAKASAGMTYSDFDGIPRQL
                     ITEAGYGSRFTHGIGHGIGLDIHENPFFGKSEQLLQAGMVVTDEPGIYLDNKYGVRIE
                     DDLVITKTGCQVLTLAPKELIVL"
     gene            complement(13477..16305)
                     /gene="uvrA"
                     /locus_tag="ACGEUI_000980"
     CDS             complement(13477..16305)
                     /gene="uvrA"
                     /locus_tag="ACGEUI_000980"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFF8165824.1"
                     /translation="MQNKIIIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSAEQIVEQVLALPERTRMQILAPVVRRKK
                     GQHKTVFEKIQKDGYVRVRVDGDIFDVTEVPELSKSKMHNIEVVIDRLVNKDGIRSRL
                     FDSVEAALRLGDGYLMIDTMDGNELLFSEHYSCPVCGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDLDLVVPDPSKSLREGALAPWNPISSNYYPTMLEQAMASFGVDMDTP
                     FEALTEEERDLVLYGSGDREFHFHYVNDFGGERNIDIPFEGVVTNVNRRYHETNSDYT
                     RNVMRGYMNELTCATCHGYRLNDQALCVHVGGEEGPHIGQISELSIADHLQLLEELEL
                     TENESTIAKPIVKEIHDRLTFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIESLKKMRDLGNTLIVVEHDEDTMMQADWLIDVGPGA
                     GEFGGEITASGTPKQVAKNKKSITGQYLSGKKFIPVPLERRSGNGRFIEIKGAAQNNL
                     QSLDVRFPLGKFIAVTGVSGSGKSTLVNSILKKAVAQKLNRNADKPGKYHSISGIEHI
                     ERLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVPCEVCHGRRYNSETLEVHYKGKNIAEVLDMTVDDALV
                     FFSAIPKIARKIQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTDDIARLLKVLERFVDDGNTVLVIEHNLDVIKSADHIIDLGPEGGDGGGQ
                     IVATGTPEEVAQVKESYTGHYLKVKLQQ"
     gene            16508..17452
                     /locus_tag="ACGEUI_000981"
     CDS             16508..17452
                     /locus_tag="ACGEUI_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFF8165825.1"
                     /translation="MKQMFLSSAIEFKEIETFEPGAWIKLVNPSQEESMKIADQFNID
                     ISDLRAPLDVEETSRIAVEDDYTLIIVDVPIYEERNNKSYYITMPLGIIVTENAVITT
                     CLHDMTLFDHFHNRRVKNFYTFMKTRFVFQILYRNAELFLTALRTIDRQSERLEAQLE
                     AATRNEELIDMMELEKSIVYLKASLKFNERIVKKLSSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMAGIYENVLNAMTETTASIINNNQNTIMKTLALMTMALDIPTVIFSAYGMNF
                     QNNWLPLNGLEHAFWYITLIAMLLSSFVVIYFIRKKWF"
     gene            17585..18241
                     /locus_tag="ACGEUI_000982"
     CDS             17585..18241
                     /locus_tag="ACGEUI_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFF8165826.1"
                     /translation="MELQELTKKNQEFIHTATNKLIQDGKSDEDIKLILEEAIPAILE
                     NQKKGVTARNLLGTPTAWAASFSQDPSQKAAETDKNTNPWLMWLDTSLLFIGIVALLN
                     GIMTFFNTNATVTGLISLLALGFGGGASMYATYYFIYRHLGKDKSLRPSWFKIIAALS
                     LAMLIWIALYSATAFLPTSLNPQLPPLALLIIGGVSLALRYYLQRKYNIQNTMSPVNK
                     "
     gene            complement(18374..18613)
                     /gene="rpsR"
                     /locus_tag="ACGEUI_000983"
     CDS             complement(18374..18613)
                     /gene="rpsR"
                     /locus_tag="ACGEUI_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFF8165827.1"
                     /translation="MAQQRRGGFKRRKKVDFIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(18778..19269)
                     /locus_tag="ACGEUI_000984"
     CDS             complement(18778..19269)
                     /locus_tag="ACGEUI_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8165828.1"
                     /translation="MINNVVLVGRMTKDAELRYTPSQVAVATFTLAVNRTFKSQNGER
                     EADFINCVIWRQPAENLANWAKKGALIGVTGRIQTRNYENQQGQRVYVTEVVADNFQM
                     LESRATREGGSTGSFNGGFNNNTSSSNSYSAPAQQTPNFGRDDSPFGNSNPMDISDDD
                     LPF"
     gene            complement(19291..19581)
                     /gene="rpsF"
                     /locus_tag="ACGEUI_000985"
     CDS             complement(19291..19581)
                     /gene="rpsF"
                     /locus_tag="ACGEUI_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFF8165829.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKDWE
                     KRRLAYEINDFREGLYHIVNLEATDAAALNEFDRLSKINGDILRHMIVKLDA"
     gene            complement(19754..20047)
                     /locus_tag="ACGEUI_000986"
     CDS             complement(19754..20047)
                     /locus_tag="ACGEUI_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165830.1"
                     /translation="MFQKLKNLIDRYSWFVFIMMILVLFLNYIYPFDPSLSGLQLEWL
                     IMTQVFPGLGIVCAMVAIIFKKFNLFVLALILYFALWLKLFLAFSFFTYFLGV"
     gene            20215..21369
                     /gene="mutY"
                     /locus_tag="ACGEUI_000987"
     CDS             20215..21369
                     /gene="mutY"
                     /locus_tag="ACGEUI_000987"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFF8165831.1"
                     /translation="MIELKDYGINMWDNETIASFRRTLLEWYDQEKRDLPWRRTTNPY
                     YIWVSEIMLQQTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNM
                     QKAAQQVMVDFGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMAR
                     LFEVNYDIGDPKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIR
                     FFNAAYLNGTYGKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFP
                     IIETSPLSQQLDLFDDNQSNPIIWQTQNETFEREYQLKPQWTDNHFPNIKHTFSHQKW
                     TIELIEGVVKATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            21445..22032
                     /locus_tag="ACGEUI_000988"
     CDS             21445..22032
                     /locus_tag="ACGEUI_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8165832.1"
                     /translation="MEIGQQIIRYRKQQALSQEKLAEKVYVSRQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFKRDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQPYKEILAVSNGKLLDEIEKREE
                     RATLPYQKPLIVTVFFLITVAFAFASRFMFTWLFH"
     gene            complement(22084..22416)
                     /gene="trxA"
                     /locus_tag="ACGEUI_000989"
     CDS             complement(22084..22416)
                     /gene="trxA"
                     /locus_tag="ACGEUI_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFF8165833.1"
                     /translation="MKWRKKMALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPIL
                     EQLSQEIDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQL
                     KAIIAELS"
     gene            complement(22479..22979)
                     /locus_tag="ACGEUI_000990"
     CDS             complement(22479..22979)
                     /locus_tag="ACGEUI_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFF8165834.1"
                     /translation="MESYEHFYAKLSQPFRKTPQLIILLNVLLKIITGVMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRFDFPRPYEKWNIKPLIDKDTKGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSALLAICRVIAGIHYPKDVIVGYLIGLML
                     GLCLLI"
     gene            complement(22983..25322)
                     /locus_tag="ACGEUI_000991"
     CDS             complement(22983..25322)
                     /locus_tag="ACGEUI_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFF8165835.1"
                     /translation="MNNKILEQLEFNKVKELLLPYLKTEQSQEELLELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSTDLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEEGRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPKISNDSTLALINVRHPLLSNPVANDLHFDHDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSHIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFSLVRVQEEQ
                     KVKNKQINVVKKADGSGPRARLDLRGKRYEEAMQELDHFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            complement(25471..26016)
                     /locus_tag="ACGEUI_000992"
     CDS             complement(25471..26016)
                     /locus_tag="ACGEUI_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFF8165836.1"
                     /translation="MLSLLIVLILTWNFYIGYSRGIILQSFYVLGALLSLLVANRFYI
                     GLAHKLTLWIPYSNPVEGTSVFFFKSVDIFVLDKVYYAGLAFFIIFLLGYALSRFLGI
                     FVHFLLLNYFDNQWTKCLSGGLAFLVSLLFLNMLLSIFATVPMPFLQHYLHSSFLTRL
                     VIEHLPPLTIIIQKLWIQAII"
     gene            complement(26019..26327)
                     /locus_tag="ACGEUI_000993"
     CDS             complement(26019..26327)
                     /locus_tag="ACGEUI_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165837.1"
                     /translation="MKSINRYKFTFGEKTLTLTTDKDNLFMEEVERVAKEKYQALKNH
                     LPEADDETIAILMAINTLSTQLSREIAIEKMEAEILDLRQKTLVGLQEKANQIDVNEV
                     "
BASE COUNT         8027 a         5689 c         4658 g         8081 t
ORIGIN      
        1 atcaatcatc gtagtcacct catctatagt gtactacttt gattgatttt gtcattatga
       61 atctagtttt ctgaaaacaa aagtttcttc atgcgtttgg cgtgggtaaa gttatcagag
      121 aaacagagag tcataatttt atcctttttt ctcatattga taccatggta ttaaacgagt
      181 tgattggggt gactcacaat catttctaat tgaccatcaa atgaccacga tttaacatcg
      241 ttaggcaaga taaagtgcat gcctttttct agctcataag ccttctggtc cacatagagt
      301 tttccttgac cttttaaaac actcactaaa agataaggcg cagcttgttt catgtcaacc
      361 atttgactag ttacccattt gtaaacagtg aaaaatggtg ttgagactaa agttgttgct
      421 accatattat ccaaaaccat agtggctggc acgctatttt ctggcttacc aatcgttaag
      481 acatcaatag acttttcaat atgaaggtca cgaagattac cattaacgtc cttacggtca
      541 aaatcataaa cccgataagt ggtatcagat gactgctggg tttctaaaat aagaattccc
      601 ttgccgatcg catgcatggt gccacttgga acatagaaaa aatcgcctgc tttgactggg
      661 acacgagtca gcaaatcatc ccaagcgcct gcttcaatca tggcacgaag gtcttctttg
      721 gacttggcct gatggccgta tacaatttca gaaccctctt cggcagaaat aatataccag
      781 cattctgtct tgcctaattc tccttcatgt tcacgtccat aagcatcatc aggatgaact
      841 tggacactta gccagtcatt agcatctaaa atcttagtta acagtggaaa aacttcttcc
      901 tttggattcc caaacaaggc cggctcttgc gcatataatg tgtttaaagg ttgtccttga
      961 taacgtccat tagtaactgt cgaaacacca ttaggatgag cagaaatagc ccaatactca
     1021 cctgtggtgt cacttgggat gttgtaagca aagacatctc tcaacttggt gccaccccaa
     1081 atcctgtcgt gcatggttga ttttagaaat aatggttctg acataatatt cctcgcttct
     1141 gatgttaatc tctgtttatt atagcatgtt ctagctgact gcgctttctt ttactagaca
     1201 agcaggcaaa aacagaccct attttccttg tgcagccaac ttggccaaag caaaatttcc
     1261 cagagtagcg gagccattat cagcaacagc tggagtaaca atgtagtctg tcaaatcagg
     1321 gactggcaaa taaccgctta aaagggcagt gaatttatca tgcacgcgca acaccatatg
     1381 ttcttgcgcc atgacacctc cgccaaaaac aatcacttga ggacggtaaa gcatagttgc
     1441 ttgcagcgct gcttgggcaa tgtagaaggc ttgaatgtcc caaacatcag cttcctgatc
     1501 aagccgttca cctctcactc ccgttctggc ctcaatactt ggacctgctg ccatcccttc
     1561 cagacaccct ttatgaaaag ggcaaacacc taaaaatccc ttggccatat catcaggatg
     1621 gggcatgaca taggtatgac ccgcttctgt atgacctaaa cccccaataa agtggccatg
     1681 ttgaatagct cccgccccaa taccagtacc tatggtgtaa tagaccaaac tttccacacc
     1741 aggacgagca agcacctcac cataagcaga gctattaaca tctgttgtca catcaaaagg
     1801 aatcttaaaa gcagccgaaa gttgtcctaa caaatccaca ttagcccagc ctgatttagg
     1861 agtggttgtg atataaccgt aagtctctga cgacggatca atatcaattg gtccaaaaga
     1921 accaatcgcc attccagcca aatctgcctc aaaagcttta aaataagcga tggttcgcgc
     1981 gatggtttct tctggtgtgg tggtcggaaa ttgtgtttta tccaccactg taaactcctc
     2041 atcacccacg gcacagacaa actttgtccc accagcttca atgcttccat ataattttcc
     2101 catgttttct cctttttctt gttctttttt atctttatga tgacacaggc tcttatcaag
     2161 acagccatgt ctcacggaca agcgccactt tctggtagtc taacgattat ttagctttat
     2221 gtcatcaaaa cctataaaag actaaaaatc tgggactatt catctcagat ttttagtctt
     2281 ttggttaatc aattccagca aacctttatc atagtcacat tttgttaatt taatctgaat
     2341 gttgctatct aagaaagtta ctttgtttat ttttttagca gctgcctttt ctcaatacaa
     2401 tctttcaaaa ctccagcatt agcattaata gcagttacat caaaaatctt actgttcaaa
     2461 gaagtttcta aagccatctc ttttattttt aactcatcaa gcatcttttg agaaaactta
     2521 tctttttgtt gagataactc tttagcagta gttactgttt tcatgatagt gtcggcgtta
     2581 ggtaacggat aacctaaaat ttggagttca gggactactg gcgaactata tctatctcct
     2641 ttagtatcaa agacaactcg ccaatattta ctagtaatat tatttaatgt attactgaat
     2701 gcagtagctc tctctccttg tgcactgtaa gtatctacag taatccaata tttttcatca
     2761 tcaaatttat tgggattttc caacaaatta tctagattat aatctttgat attaaaaatt
     2821 tgtagacttg cttcctgaat aggtttattg gttgtctcag gatttcgggc tgaatcattg
     2881 aagaaacgcc aatgctttat taatccatct tctttcaatt taaatataat actttgctta
     2941 gaatcccatc ctaaagagat attatcagtc tcactgccca gttgcccatc aaataccttt
     3001 cttgcattta caggatcagc acttcctcca ataactgttg cgccttctgc caaattaacc
     3061 atcatggttt tttcgtcacc aacaatcact ttagcagtat gaacagcttt tgtcttatct
     3121 gctgggctaa agaagtcaac cttataggtc tcttctttat cagttgctag cgttttgtca
     3181 gtagtggttc ccaatgtgga atcagttact ttaacggtat agttctcata agaaacttta
     3241 aagttattgt aatgatagtt tgaatcaaca aagctacgtc cagcaatttt atgattttgg
     3301 taagccttat agtctgcttc gctattgatt agggttcctt gatttgtcac agtcccaaac
     3361 aaaagctggc tttgcaaatc aactttttca tttgccactg gtaagcgcag actagttttc
     3421 ccataggtat ctgggtaatg cccagttggt ttttgcttgt caaatttcac tgtttgttca
     3481 ttgcttccaa catgattgct gatagttgat agcatagtat caaaaatttg tcgattttct
     3541 gttcctggag ccaaatcaag tttgttgcca gaaatatcaa ctttttctaa agacgttaga
     3601 gtagcggcat caagaccagc caaggtttca cggtcaaaac ctgacaaatc taattctttc
     3661 agaccagtta aaccagaaac cttcaaagat actggtggga tagtagcagg ttcttctttg
     3721 ttatcctttt tataggtttc aagaactgtt tccaaggtat ctttgcctgg cttcatatta
     3781 gcgggtaaaa cagaacggtc gagctttgta atgcgagata agccaatcaa gtctaattga
     3841 gctaattttt taaatttatt tagaccttct aaactttgaa tcgctggatt atccaatcgt
     3901 aatgtgccat tgaaacgttc caaatcacct tttctggttc caacctgcgc catcacagct
     3961 tctcgcaaag ccttatctgg gaaatctttc tcatcaatca gatcatacga cttatctttt
     4021 agcataactg tctttaatgc cttggagaca ctataatctg agtggaagat gttatcagtt
     4081 gcgtccttaa actctttctg tttagcatat tttttaggtt gatgagctac accatctcgg
     4141 tcaatagcgt aggagaagat acctccctta accccacctg tcttaggttg ccaccttgca
     4201 taccgttcgg cacgcgttcc agttatgtca gtgttaattc catttgcttt gtcctcgtcc
     4261 ttgcgagaat taatatcata ccaaagattc ccttcttgag cattttcctc atagaaagaa
     4321 aaaccaatca tgtattgttc aggacgaata tacttgctat aaccttgcca tcgttcttcc
     4381 attgtttttt caggtcgatt agaaacaggc tcccaaccac ctttctctcc ttgtgaacca
     4441 tagacctgta ccagtaataa attaatataa ggagctcctc gctcaatcaa tgggttttta
     4501 tcagccatgt aggtgctatc cataataaat aaccgcgatt tatcaacacc ttttggtcca
     4561 attaatttcc caatttcttc aaacacttga atagagcgtt ctacgcctgc tgtatcttct
     4621 tttttgtcaa cttttggaat actatcatgt tcaacatcca catctaagcc atcaaggttg
     4681 tatttataaa catattcatc aacaatagct ttggctaaag ctttatttcc ctctggtgta
     4741 tttgggtatt tactggtatc ttctgcaata ccactgttat cacccccagc taggaaacgc
     4801 catggaatgg tacgaatgac acgtgtccct tgcttgttta actttggcac atgtttggtg
     4861 gccaattctt tccaaaaaag gctataatct tttgtccaat cgtggaaaat aaaggctaga
     4921 tctacttctt taggaagctc tcccatcgag ttaactttgt ctttttctgt tggatctgat
     4981 gttttgtcat gccaagttct aaagtaacca ccgtagagag gaccatgtaa cggtttcatc
     5041 ggtattttct caggaatttt cattttggca agttcttgag cttgtttatc tgcctgctga
     5101 gcttttgcta atatctcttt gaccttttga gattcttgcc ccgcttttga gagttcttct
     5161 ttaaattctt ttttgctatt ctctgacaga taatgcaagc tatcgataga aggtaatcct
     5221 ttctgaacct gaacagtctt ctcctccgct tttacagtat tgagtgaatc atggtgggtc
     5281 gctaaggcag ctcccatcaa cgttgcagca caaacacacc ctagtgttct ttttaccaac
     5341 aaatgtttat ccatttggac actccttatt tttggtacta agtcatggct tcccagttga
     5401 tataccatcc atgtaagcgc attcctatag ttcgccttaa gtataacaac actttattaa
     5461 aaaaaacaag gtacaaagta taggtaaaat aacacaaatt taaggttttt aggcttttct
     5521 catttttttg ccaacctgcc atccatggca ggacaaaaga gctgcttagt tagctctttt
     5581 gtccttattt gataagtgcc acttgatcac caatcaaaac atgtccttga gcaagaatgt
     5641 ccacgctttg atagtcagcg ctattggtca caatcatcat ggtggtgtca tctagtccag
     5701 cttctgcaat cttgctagga tcaaagtgtc caagaagatc acctttttta accgcctgac
     5761 ctactgctac aagagattca aagccatctc ctgccatcga cacggtatca ataccaatat
     5821 gaagtaacac ttctgctcct tgacttgacg ttatagcata ggcatgacct gtttcaaaga
     5881 caatttcaac tttaccatca actggtgagt atagggtatt atcttctggc ttaattgcta
     5941 agccttgacc catagcacct gatgaaaaaa cagggtctga aaccgcagat aagtctacaa
     6001 ctgttccatt caaaggacta tataaagttt cctctgctaa ggctggctga tcagccgttt
     6061 gatcaacttc gacagctggt aacggaaggg tttctctatc ttgataaccc caagtataag
     6121 caattgcaaa ggctacccca agaccgacaa gcatagtcac aaggtattgt aataattggc
     6181 cattcaagta aaggagagtt cctggtaaga ccgtaatccc aaaaccagtt cctgcaatgc
     6241 caaagagccc agcgacccaa ccacctaagg caccaccaat aagacctgaa acaaagactt
     6301 ttggataacg gaggttgacc ccaaaaatag ctggttcagt aatccctaaa agagctgaca
     6361 aggttgatgg aaaggccaga ccttttagtt ttgttgattt ggttttaaca gcaacagcta
     6421 aggtagctcc agcttgagca gctgttgctg ctgttaggta agcattgaaa ggatcttttc
     6481 cggtattggc aatcagctgc gcttctagga agttaaagat atggtgaata ccagtcacta
     6541 cgattaattg ttggattccc ccaacaatca agcctgcaat gccaaacggc aaatgcaaga
     6601 cggcctgtgt cccagccaga acaaggtttt caagagaatg gaaaactggt ccaatcacaa
     6661 atagtcccaa ggtactcata atagcaaacg ttaaaaacgg tgtgaccaat aagtccaaag
     6721 cttctggaac ctttttgtgc aaccatttct ccaacttagc tcctaccaga ccaacaaaga
     6781 aggcgggtaa aacggtacct tgataaccaa caacaggaac aaatccaaag aaggttagcg
     6841 gcttaacatc tccaccagag gcaactaccc aagcattagg caactcattg gaaaccagca
     6901 tcaatcctaa aacaataccg ataataggat tacccccaaa gaccctaaag gctgaccaag
     6961 ccaccaaagc tggcaagtag acaaaggctg tatccgttag aatacgagtg tacatgagaa
     7021 aattttcccc gtactcatgc accccaaata aatccataat agctggctgg gtcaccaaac
     7081 cacgaacccc cataaagaga cccgttgcta caatagctgg aataatggga acaaagacat
     7141 ctccaaacgt acgaatcgcc cgttggaaga tattgccatg tttgcctgct tctgcctttt
     7201 gctcactggt tgatgacgtg ggtaaaccaa gagcaacaac ttcgtcataa atgttattaa
     7261 cggtaccagt tccaaaaatc atctgatatt gaccagagtt aaagaaagct cctttaacct
     7321 tgtcaatagc ttctgctttt tccttatcaa tctttccttc atcataaacc atcacgcgaa
     7381 ggcgagttgc acagtgggca acacttctca cattttctcg gccacctaaa gcctcaatca
     7441 cttcagctgc aatctgacga ttatccatgc ctaaaagtct cctttttcat ttggtgttag
     7501 tccctcatcc attctgccag ctttgtggct aagaatgtgc aggaccaacg ctagtgaatc
     7561 cgcttcactt cttgactatc attttatcac taatattttt tatgtcaagc gtttaacata
     7621 tttttatttt atatgcttta aattcttgat ttttaaacag aaaacgttta gtatattctt
     7681 gaagacgact ataaaacaag gagattttga tggatctacc acaagctatt cgctaccgcc
     7741 cttacaaaga gtggagctca aaagactacc aagctattac tgaaaaaatg gcccaatccc
     7801 catggcacag ccaatttcat gtcgaaccta agacaggatt actcaatgac cccaatgggt
     7861 tttcttattt caacggtcgc taccatcttt tttatcaaaa ctggccttat ggcgctgcgc
     7921 atggtctgaa acaatgggta cacatgacat ccacagacct cgtccatttt acggaaacaa
     7981 ggagccgcct tctgccagac cacgctcacg atagtcatgg ggcttactca ggttctgcat
     8041 atgctattga tgacaagctc ttcctctttt acactggtaa tgttcgcgat gccaattggg
     8101 tcagaacacc attacaggtt ggcgcatgga tggataaaca aggaaatatt tccaaaatac
     8161 cacaggtttt gattgaacag cccgatgatg ttacagaaca ttttcgggat cctcagcttt
     8221 tttcttatca aggtcaattc tatgctatta ttggcgccca aggtttagat ggcaaaggaa
     8281 aaatcaaact ttacaaagct gtggacaatc atgtggacaa ctggcgattt atagccgatt
     8341 tggactttga cgattctggt acggaataca tgattgaatg ccccaacctt gtgtttgtcg
     8401 atgacaagcc tgttttgatt ttcagcccac aaggactggc taaagctgat ttagattatc
     8461 aaaatatcta ccccaatacc tataaaattt ttgaaagctt caatccagaa actggccaat
     8521 tgctgggtgg aggggccctt caaaatcttg actttggttt tgaagcttat gccactcaag
     8581 cctttagtag tccagatggc agggttctag ccgtgtcttg gattgggtta ccagatattg
     8641 attatccgac agatcgttat gactatcaag gagctcttag tttagtgaaa gaattaagga
     8701 ttaaagacgg aattctctat caaacacctg tttcagccct acaaaatctt cgagggccag
     8761 cagaattatt tcataataaa atagacagtt ctaactgtta tgaattggaa ttgacgattc
     8821 caggccaaaa aaagcttgac ctgctcctat ttgccgacca aaaagggaat ggcctaagat
     8881 tgaaagtgga tacaacaaaa ggacagttaa gcattgaccg aagtcgggca ggggttcaat
     8941 atgcccaaga ctatggtacg gtgcgctctt gccaaatccc acaaggccat gttacactta
     9001 atgtctatgt ggacaattcc attcttgaaa tctttattaa ccaagggcaa aaagtcctaa
     9061 ctagtcgggt tttcccaacc cacggacaaa caggtatcca agtagtagaa ggacaagctt
     9121 ttggacatta ttatgaaatg aggtattagg caagtggtcg caaaattaac agacgtggca
     9181 gccttggcgg gtgtcagtcc aacaactgtt tcgcgagtaa tcaacaaaaa aggctacctt
     9241 tcccaaaaaa cagtgaataa ggtgaataag gccatgcgtg aattgggcta caagcctaac
     9301 aatctcgcta gaagtttgca aggtaaatca acccaactca ttgggcttat cttccccaac
     9361 attagcaata tcttttacgc tgaattaatt gaacatttgg aaattgaatt gtttaaacag
     9421 ggctacaaaa ctattatttg caatagtgaa cataatcccg tcaaagaacg cgaatattta
     9481 gaaatgcttg cggccaatca agtggacggc attatttctt ccagtcataa tttgggaatt
     9541 gaggattatg agcgtgtcga agctcctatt gtagcttttg accgtaatct agcgccaaat
     9601 atcccggtta tttcttcaga taattttgaa ggtggtaaat tagccgcaca aactttgcaa
     9661 aaacacggtt gtcaaaacat tgtcatgatt acaggtaatg acaattctga ttcgcccaca
     9721 gggttgcgcc agctagggtt taactaccag ctcaaacgaa gtgcagaaat catcaaatta
     9781 ccaaataacc tatctcctgt tagacgcgaa atggaaatta aatccatttt ggcaacccga
     9841 aaacctgatg gccttttcgt ttctgatgat ttaactgcta tcctcattat gaaagtagcc
     9901 aaacaacttc acattactat tccagaggat atgaaagtca taggttatga tggaacgaca
     9961 tttatccagc aatatgttcc tcaattagcg accattcgtc aacctattga tgagattgct
    10021 aaattgagtg tcgaaatact catcaaaaaa attaaaaaag aaaaaaccag taaagactat
    10081 attttaccga ttacgctact tccaggagca agtatttaaa caaaacataa ccaatcacta
    10141 attactctaa aagacaaaca agcttactct ttctagatgc gagagtatgc ttgtttgtct
    10201 tttaatattc gtacataaat tgaaaaaatc ggatacaatc ctaagattgc tccgacttat
    10261 tatgatttat ttgctgaagg agctcccgag acatattggc taagtaagcc attaataaat
    10321 ttagcagagg tttcatcaga atatttttta gctacttcaa tgatttcgtt tagtgcaaca
    10381 cgatctggtg tttcgtcaaa atacttgatt tcaaaaagac ctagtcgtaa taacgttttg
    10441 tctgttagtg tcaagcgttc taacgaccaa ccttttttca aatgagttga tatcaagtta
    10501 tccaactctt ctttatgatt attgacaccc gtcacaagac ttaataaaaa aatcggcagc
    10561 tccaatactt gagcgtcctc ccctgttact ttatcataac cataggcaaa ctgagaagct
    10621 gccaataact cagctcccat ctctatgtta aataaagcct gaaaggcacg ttcacgcaaa
    10681 tctcttcttg aattttgaaa gctgttagtc atccaagaaa tcctcatcaa aaagtgactt
    10741 caaatctggt tttggtgtct tttccgcaac aataccttca acatggatat taactgcaga
    10801 aataggcact tctgccatat cgtacacagc agatttgact gttttttgaa tattcataga
    10861 tacagtcggg accttgactc catattgcaa gtaaacataa atatctgctg taacacttcc
    10921 atcttcttct gtctggagat aaaccccttt accaagactt gctttgttaa agctatctgc
    10981 cattttcttg ttatggagtg agtggacgcc ctcaacttgt gtcgtagcaa tccccgtaat
    11041 aacctcaagc actcgtggtg aaatgacgat ttcaccgata tattcagttg tcatagcatt
    11101 tcctttctct tttttagact aatctgtttt gacaaaggat taagcacgag aaacgtaagt
    11161 accttctgca gtgttaatga ttagtttttg gccagcttcg ataaagtctg gaacgttaac
    11221 aacaagtcct gtctcaagag ttgcaggttt ccctgaaccc gtcactgtcg ctcctttaat
    11281 agatggttgt gtttccgcaa cggtcaattc aacagttgtt ggaaccgtta ccccaatcac
    11341 ttcacttcca taaaattgga ttttcacgtc tgagttttca agaatgtaaa gcaattcttg
    11401 ctcaacgtta gcaactggaa tttcgtactg atcataagtg tcagtgttca tgaagtaagc
    11461 agtgtcatcc attttgtata ggtattgtgc tgggacagtt tcaatgatgg cttgctcaaa
    11521 tttttcatct gggcggtaag ttgtgtcaaa agtagaacct gtacgcacat cacgtagttt
    11581 catacgcatg atagtgtttc ctttacctgg tttgtggtgg ctagcttcaa ggacacggat
    11641 taattttcct tctgcttcaa atgtcatacc tgctttaagc ttacttgctt caatcattat
    11701 tattacctct tactaaaata tttatcactt cattttacca caaaagtggc cttggctcaa
    11761 atcttttatg accgtccttt taggtacact ttctattgtt ccttatcgcc tcctaaaaca
    11821 atttgaggca catcatgttg cacataggca accccttttt tctccatcag ttcaatggca
    11881 aaggggtgcg gacggtaatg tgctttatag gtaatctttg taatccccgc ctgcaaaagc
    11941 gccttagtgc agttaataca aggaaaatga gtcacgtaaa tttcagttcc gtccgttgaa
    12001 attccttctt tcgcacattg aatcaaagca ttcatttcag catgaacagt gcgaatacaa
    12061 tgtccgtctt ccatataatg acccgcttcg ttgcagttat ccgtagcaga aactccgcca
    12121 ttgtaacctg tggcaataat gcggttatcc tttaccaaaa cagctcccac aaaggcgcga
    12181 tcacaggtag agcgcttgga aatcagctct gcattggcca taaaatagtc ttgccatgat
    12241 aaacgatttg tcattgcttc tcccctaatc ctttcctgaa ccattctttt acaatacaat
    12301 taattctttg ggtgccaagg tcaagacttg acaaccagtt tttgtgataa ccaagtcatc
    12361 ttcaatacgg acaccatatt tgttatccaa atagatacct ggctcatctg ttaccaccat
    12421 tccagcttgg agaagttgct cagatttccc aaaaaatgga ttctcatgga tgtcaagccc
    12481 gatgccatga ccaatgccat gtgtgaagcg actgccataa cccgcctcag tgatgagttg
    12541 gcgcggaata ccgtcaaagt cactataagt catgccagcg ctagctttag caattaaagc
    12601 cttattagca gcaagaacaa gagcataaat ctcacgttct tcatcagtaa cttggccaat
    12661 atgaatggtc ctcgtcatat cactaacata gtgattgtag taacacccaa agtccatggt
    12721 caagctctct ttattctgga taaccttgtc actggcgcgt ccatgaggca tggcagagag
    12781 atagcctgaa gctacaatga tatcaaatga tgtgccgctg gcaccatact gacgcatacg
    12841 aaaatctaaa aaattagcca ggtcacgttc agtggttgtc cctggtttaa taaaatcaag
    12901 agcatcttca aatgctttgt ctgagatcga gcacgcttta gcaatggtat cgatttcaga
    12961 ggcgtcctta ataagacgta aatgctccac aaaacctgac tgagcaagca aggttattcc
    13021 tgacagttct gcttgcatgg cctggtaaaa agaaaacgat acctggtcct caaaaccaag
    13081 gcaatctatt tgatcagcct ctaacaattc tgccacaacc ttaagcggcg tgcggctttc
    13141 gataatatca aatccctcaa cactagcttt agcaagcaag gtataacgtg aatctgtgat
    13201 caaaacacga cgtttggccg ttatcaaaac agttgctgca gttccagaaa aacctgtcaa
    13261 gtaataacta ttggttaaat gggtgactag aagagcctct agccccttct ctgccatctg
    13321 ccttaggcag tgacctaatc gttgttctaa aaatcctgac atcgcgaact cctttggtaa
    13381 cttttgagaa tattgtagca tgaaaaaact actttaagtt taccatgatt tgagagcatt
    13441 taaaagatta aaaggagctc aaacgaactc ctaatcctat tgttgtagtt ttactttcaa
    13501 ataatgacca gtatagcttt ctttaacttg agctacctct tctggtgtcc ccgtggcaac
    13561 aatctgacca cctccgtcac caccttctgg tcctaagtca ataatatggt cagcagattt
    13621 gataacgtcc aaattatgct caatcacaag aaccgtatta ccatcgtcta caaagcgttc
    13681 caaaactttt aatagccttg caatatcatc tgtgtgtaaa ccagtcgttg gctcatctag
    13741 gatatacaag ctcttacctg tcgagcgttt gtgcaattca ctagccaact tcatccgttg
    13801 agcttctccc ccagagaggg tggtagctgg ttgacctaag gtcacataac caagtccaac
    13861 atccttaatc gtttgaattt ttcgagcgat tttaggaata gcagagaaaa ataccaaggc
    13921 atcatcgaca gtcatatcaa ggacttctgc gatatttttt cctttgtaat gcacctcaag
    13981 cgtctcagaa ttataccgtc tgccgtggca aacctcacaa ggcacataaa catctggcaa
    14041 aaagtgcatt tcgattttga taatcccatc acctgaacaa gcctcacaac gcccaccttt
    14101 gacattaaaa gagaaacggc cttttttgta accacgaatc ttagcttcgt tggtttgggc
    14161 aaacaagtct cgaatatcat caaagacacc cgtataggtg gctggatttg accgtggcgt
    14221 ccttccaatc ggactttggt caatgtcaat caagcgttca atatgttcaa taccagaaat
    14281 agagtgatat ttaccaggct tatcagcatt acggtttaat ttttgggcga cagccttttt
    14341 caaaatacta ttaaccaagg ttgatttccc tgagcctgat acccctgtga ccgcaataaa
    14401 tttacccagt gggaatctca cgtcgaggct ttgaagatta ttctgcgctg ctccttttat
    14461 ttcaatgaat cggccattac cagaacggcg ctctaaaggc actggaatga attttttacc
    14521 agacaaatat tgacccgtga tggatttttt attcttagcc acttgtttcg gcgttccaga
    14581 ggcagtaatt tctccaccga actcacctgc ccccggtccc acatcaatca gccagtctgc
    14641 ctgcatcatg gtgtcttcat cgtgttctac cacaattaac gtattaccca aatcacgcat
    14701 ttttttcagg ctctcgatca aacggtcatt gtctctttga tgcaatccaa tagaaggctc
    14761 atccaagata tagagcaccc ctgataggtt agaaccaatt tgtgttgcta acctgattcg
    14821 ttgactttca ccacctgata aagtcccagc agcccgtgac aaggtcagat aattcagtcc
    14881 cacattattt aagaatgtca gacggtcatg gatttctttg acaatcggtt tagcaatggt
    14941 actttcattt tctgtgagtt ccaattcttc caataactgc aagtggtcgg caatcgataa
    15001 ttctgaaatt tgaccaatat gaggtccttc ttcgccaccc acatgcacac acaaagcttg
    15061 gtcattcaaa cggtaaccat gacaagtcgc acaggtcaat tcgttcatat aaccacgcat
    15121 cacattgcgc gtataatcac tattagtttc gtgataacgg cgattaacat tagttacaac
    15181 accttcaaaa ggaatgtcaa tgttacgctc accaccaaaa tcattcacat agtgaaaatg
    15241 aaattctcta tcacctgaac cataaagcac taaatctctt tcttcctcag tcaaggcctc
    15301 aaaaggtgta tccatatcca ccccaaaact tgccatagct tgctcaagca tggttgggta
    15361 ataattggag gaaataggat tccaaggcgc caaagctccc tctctcaaag acttacttgg
    15421 gtcaggaaca accaagtcca aatccacttc caacttaatg cctagcccat cacaggtcgg
    15481 acaagagccg aacggcgcat taaaagagaa gagtcttggt tctaattcag gaaccgtaaa
    15541 tccacagaca ggacaggaat aatgctcaga aaagagcagt tcattgccat ccatagtgtc
    15601 aatcataagg tagccatctc ccagccttaa agctgcttca accgaatcaa ataaacggct
    15661 acgaatacca tccttattaa ccagacggtc aataaccacc tcaatgttgt gcatcttact
    15721 tttggacaac tctggcactt ctgtcacgtc aaaaatatcc ccgtcaacgc gcacacggac
    15781 atagccgtct ttttgaattt tctcaaaaac tgttttgtgt tgccccttct tccgacgaac
    15841 aacaggagct aaaatctgca tgcgtgtccg ttcagggagg gctaagacct gctctacaat
    15901 ttgttctgca gaagacgccg tgatagcacc atgcccattg atacagtaag gtgtgcccac
    15961 acgcgcatat agtagacgta aataatcatt gatttccgta accgtaccaa ccgttgagcg
    16021 agggttctta ctggtggttt tttggtcaat ggaaatagca gggctaagac cgtcaatgga
    16081 atccacatct ggtttttcca tattacccaa gaactgtcta gcataagctg ataaactttc
    16141 tacgtaacga cgttgccctt ccgcataaat agtatcaaag gctagactag attttcctga
    16201 ccctgataac cctgtcacga caactaattt gtctcgtgga atttccacgt caatattttt
    16261 taaattgtgg gctcttgccc catgaattat tattttattt tgcatcttga atgtgcctaa
    16321 aggcaccctc ctcatcgagc tttcttatat tataacaaaa atttctgata acctattgcc
    16381 ccaaactttg cgaattttgg tataatagat aagtattgag aatttttcag aaaatccttg
    16441 agaagacttt attatcaagg tttgatgtca atctctacca gttaatcatc aaaaaggagg
    16501 gcttgtcatg aaacaaatgt ttctttcatc ggcaattgaa ttcaaagaaa tagaaacatt
    16561 cgagccagga gcttggatta aactggttaa cccttcccaa gaagagtcta tgaagatcgc
    16621 cgatcaattt aacattgata tttctgactt gcgcgcccct ttggacgtgg aagaaacctc
    16681 acgtattgcc gtagaagatg attataccct aattatcgta gacgtcccga tatacgaaga
    16741 acgtaacaat aaaagttact atattactat gcctttaggc attattgtaa cggaaaatgc
    16801 tgttatcaca acttgtctgc atgacatgac ccttttcgat cattttcaca accgccgggt
    16861 caaaaatttc tataccttca tgaagacacg ttttgttttt cagattctat atcgcaacgc
    16921 cgagctcttt ctgactgcct tgaggaccat tgatcgccaa agcgaacgtt tggaagctca
    16981 gcttgaagca gctactcgaa atgaagaact tatcgacatg atggaacttg aaaaatccat
    17041 tgtctactta aaggcttcct tgaaattcaa cgaacgcatc gtcaagaaat tatccagctc
    17101 aacaagctct ctcaagaaat acattgagga cgaggatttg ctagaagata ccttgattga
    17161 aacccaacag gcgattgaga tggctggcat ttatgaaaat gtcttgaatg ccatgaccga
    17221 aaccactgct tctattatca acaataacca gaacaccatt atgaaaacac tggccttgat
    17281 gaccatggct ttagatatac caactgtgat tttctccgcc tatggtatga atttccaaaa
    17341 caactggtta ccactaaatg gcctagaaca tgctttttgg tatatcacct tgattgctat
    17401 gctccttagt tcctttgttg tgatttactt tattagaaaa aaatggttct agagtggagt
    17461 aagctgcaga atgcggttaa gacctgacaa aactctgtgt cagacgactg gctccaaagt
    17521 ctcatttgac actaactaat atcattgact tactttaact aacccatata aagaagggat
    17581 tcccatggaa ttacaagaac ttaccaaaaa aaatcaagaa tttatccaca ccgctacgaa
    17641 caaattaatc caagacggca agtctgatga ggacattaag ctgattctcg aagaagccat
    17701 tccagctatt ctcgaaaacc agaaaaaagg agtcaccgct cgaaatctat taggcactcc
    17761 gactgcttgg gcagcttctt ttagccaaga ccctagtcaa aaagcagcag aaactgacaa
    17821 aaacaccaat ccttggttga tgtggctaga tacctctttg ctattcatcg gaattgttgc
    17881 ccttttaaat ggcatcatga ccttcttcaa cacaaacgct acagttacag gattgatttc
    17941 cttactggca cttggttttg gtggaggagc atccatgtac gctacttatt actttattta
    18001 ccgtcatctc ggaaaagata agagccttcg tccaagttgg ttcaaaatca tcgcagcctt
    18061 atcattagcc atgttgattt ggattgcctt gtactctgca actgcctttt taccaacctc
    18121 tcttaaccca cagttgcctc ctcttgcctt gctcattatt ggtggtgttt cccttgcttt
    18181 acgctattac ctacaacgca aatacaacat tcaaaacact atgtcacctg ttaacaaata
    18241 acaatcattt catccttgct gtctacgcaa ggattttcgt ttactatcat tctctctttt
    18301 agaaaaaact cacctatacg cctagactag tcctagtcaa ctaaaaaaca gtccgaaatg
    18361 gactgtttgt ttattagtct tcgtttacgt aaggcataag agccataacg cgagcacgtt
    18421 tgattgctgt tgttacttta cgttggtttt tagctgaagt tcctgttaca cgacgaggaa
    18481 gaatttttcc acgttctgaa acgaaacggc taagaagctc agtatctttg taatcaacat
    18541 attcaatttt gttagctgcg atgaagtcaa cttttttacg gcgtttgaat ccgccacgac
    18601 gttgttgagc catgtatttt ctcctttata aattatgatg ttaagggctc ttaaaaaacg
    18661 actgaatttt agaaaactgg caagaaatct tgatttccta gggagttgat ctaaattcta
    18721 agttctaaat ttttagcccg aactcaaata atttcgtatt cgttcaatcc attctgacta
    18781 gaatggaaga tcgtcatctg agatatccat cgggtttgag ttcccaaatg ggctatcatc
    18841 tcttccaaag ttaggcgttt gttgtgcagg cgctgagtaa ctgtttgatg atgaagtgtt
    18901 attgttaaaa ccaccattaa atgagccagt tgagccacct tcacgtgtag cacgactttc
    18961 caacatttgg aaattatctg caacaacttc tgttacatag acacgttgtc cttgttggtt
    19021 ttcgtagtta cgtgtctgaa tacgacccgt aactccgatc aaagcacctt ttttagccca
    19081 gttcgctaaa ttttcagccg gttgacgcca gatcacacag ttaatgaaat ctgcctcgcg
    19141 ttcaccattt tggcttttaa aggtacggtt aacagcaagt gtgaaggtag ccacagctac
    19201 ttgacttggt gtgtaacgaa gttctgcatc cttggtcata cgaccaacta gtactacatt
    19261 attaatcata aactaccctc ttagttggaa ttaagcgtca agtttaacga tcatgtgacg
    19321 aagaatgtca ccattgattt ttgaaagacg gtcaaactcg ttaagagctg ctgcgtcagt
    19381 cgcttcaagg ttaacgatgt ggtaaagtcc ttcacggaaa tcgttgattt cgtatgcaag
    19441 acgacgtttt tcccaatctt ttgattcaac aacagttgca ccgttgtcag tcaagataga
    19501 gtcaaagcgt gctaccaaag cgtttttagc ttcttcttca atgtttggac gaatgatata
    19561 aagaatttcg tatttagcca ttgatatttt cctccttttg gtctaatgac tcactacttt
    19621 tggagtgagt aagtgaggta tactcacaga atcttattgt acactaatag tcaattaaaa
    19681 gcaaggaact ttttctactt gactaagttt tgtcaaacat tttctttcac gagttttaaa
    19741 aattggcact tagtcagact cctaaaaaat aggtgaaaaa agaaaaagca aggaaaagtt
    19801 ttaaccaaag tgcaaaatat aaaattaggg ccaaaacaaa aaggttaaac tttttaaaaa
    19861 taatagcaac catggcacat acgataccta atccaggaaa aacttgcgtc atgatcagcc
    19921 attccaactg tagtccgctc aaagaaggat caaatggata aatatagtta agaaataaca
    19981 caagaatcat cattataaaa acgaaccacg aataccgatc aataagattc tttaatttct
    20041 ggaacatgtc ccctccaaat taaaacctgt ctgcttctat cgttaatttc agttattcta
    20101 aatcaacatt tagatgaggt aaacctaata ttattataat atttcctttt ctttttcgca
    20161 aatacctaaa atcttttttt actcttttta agactcctgt gttacaataa caacatgatt
    20221 gaattaaaag attatggtat taatatgtgg gataatgaga caatcgcttc ttttcgtcgc
    20281 acacttttag agtggtatga ccaagaaaaa cgtgatctcc cttggcgaag aactactaat
    20341 ccatattata tttgggtttc tgaaatcatg ttgcaacaaa cacaagttaa tacggtaatc
    20401 ccttattata aacgttttct agagtggttt cctcaaataa aagatttggc agacgctcct
    20461 gaagaacaat tactaaaggc atgggaggga ctaggctatt attctcgggt gcgtaatatg
    20521 caaaaggctg cacagcaagt gatggttgat tttggtggca tatttcctca cacttatgat
    20581 gatattgctt cattaaaagg gattggtcct tatactgcag gtgctatcgc cagcatctca
    20641 ttcaacttac cagagcctgc tgttgatggc aatgtgatgc gtgtcatggc tcgtttgttt
    20701 gaagttaatt atgatatcgg tgaccctaaa aatcgtaaaa tttttcaagc tattatggaa
    20761 atactaattg accctgacag acccggtgat tttaaccagg cacttatgga tctaggtaca
    20821 gacatcgaat ccgctaagac acctaggcct gatgaaagcc ctattcgttt ctttaacgct
    20881 gcttatttga atggtactta tggtaaatac cccataaaaa atccaaagaa aaagcctaaa
    20941 ccaatgcgaa ttcaggcttt tgtcattcgt aaccaaaatg gccagtatct actggagaaa
    21001 aatactaaag ggagacttct aggaggattt tggtcatttc ctattatcga aacatcacct
    21061 ctttctcaac aattagattt atttgatgac aatcaatcca accccataat ctggcaaacc
    21121 caaaatgaaa ctttcgaaag agaatatcaa ttaaagccac aatggactga taatcacttt
    21181 ccaaatatta agcacacttt tagtcaccag aagtggacaa tagaactcat agagggggtg
    21241 gtcaaagcaa cagatttacc taatgctcct cacctaaagt gggtagcaat tgaggacttt
    21301 tctctttatc cctttgcaac accacaaaag aaaatgctag agacctactt aaaacagaaa
    21361 aatgcttaat cgcaagtgaa ctataacggt tcactttttg tttacaattc ctttacgccg
    21421 ctgtaaagtt tttcggtgat atttatggaa attggtcagc aaattattcg ctaccgtaaa
    21481 caacaagcgc tctcgcaaga aaaattagcc gagaaagtct atgtaagccg tcagagcata
    21541 tctaactggg aaaatgacaa aacttaccca gacatccata gcctcttact cctaagtcaa
    21601 atttttcaag tgagcttaga ccaactcatt aaaggagaca ttgagaaaat gaaatatacc
    21661 atcactcagg ttgataaaaa gaattttaaa cgagatacca aagtaatggt aacattgatg
    21721 atactattaa tgatttcaag ttatccatta gtctatttct tagagtggct aggacttggc
    21781 atttttgttt tattatcgat aattactatg acttatgcta atcgtgtgga acgttttaag
    21841 aaaaaatatg atgtgcaacc ttataaggaa atcctagctg tttcaaatgg aaaattatta
    21901 gacgaaatcg aaaaacgaga agaaagagca acacttcctt atcagaaacc ccttattgtc
    21961 actgtatttt tcctcattac tgttgctttt gcttttgcaa gtcgttttat gtttacatgg
    22021 ttattccatt gaagttagta ggtcataaaa taaagctcag cgtgacgctg ggctttattt
    22081 tagttaactc aattcagcaa taatagcttt caattggtct ttagtatgaa cacctgcaac
    22141 ttgtttaacc acttctccgt cttttttaaa catcaatgtt ggaattgaca taataccaaa
    22201 ctgacgagca gtttccggat tttcatcaac atccattttc aaaattttga gttcatcttc
    22261 atcgatttct tgagataact gttctaagat tggtgcttgc atacgacatg gaccacacca
    22321 agttgcccaa aaatcaatga gtactaaacc ttctttagtt tcttctacaa atgtagcatc
    22381 tgttacttct aatgccatct tttttctcca tttcatctta atgataagcc tattgtacaa
    22441 gaatttagtt gtttttgaaa ctatttgcta cgaactcgct aaatcaataa acaaagccct
    22501 aacatcaagc caataaggta acctacaata acatccttag gatagtgaat acctgctatc
    22561 acacggcaaa ttgccaataa agccgataat attaaacaaa ctattccaaa gtaaacataa
    22621 tatcgtaaca aacacatact aatcatcgta gctgaaaaaa catgtctgct aggcattgac
    22681 cttccctttg tatccttatc aattaaaggt ttaatattcc atttttcata cggtcgtgga
    22741 aaatcaaagc gtttcctaat gtaagacaaa gctataaagc caacagcagg aataattaat
    22801 aaccttaacc ataattgaaa tgtcatccct tgccaaagcg taaatatcag aaaagatgga
    22861 taaagtatgt acatcactcc tgtgattatt tttagtaaaa catttaaaag aattattaac
    22921 tgtggggttt ttctaaaagg ttgagatagt ttggcataaa agtgttcata tgattccatc
    22981 atttacccta acgttacaat tgtggcacca gatccccctg cattttgtgg ggcataagca
    23041 aaatgcttaa catgcttatt acgacgaaga tattttgtca ctccctcacg aataacgcct
    23101 gtaccaatac catgaatgat atcaacttgt cccatattgt taagcaatgc ttgatcaata
    23161 aaatgatcta actcttgcat tgcttcttcg tatcttttac ctctaagatc aagtcgagct
    23221 cttggtccag aaccatcagc ctttttaacc acattaatct gtttattttt gactttctgt
    23281 tcttcttgga ctctaacgag gctaaattca tcttgtgtta atgtcatttt gataattccc
    23341 acttgtgctt cccaacggcc atcttttaat tgactagtta aggtacctcg ctgtccataa
    23401 ctagtcacta taatatcatc accaattcta ggggcacgag ctgctttgat ttttttagcc
    23461 ttatttaaga ctttattttt tgataaatca acttgaggtg ctaatttttt tatttgtgcc
    23521 ttagcatcta taatttcgtg aggttttaat tggctcttat cattgagttt ttttaagata
    23581 gtatcactct cattcaaagc catatctaca atttcttgag cttcttgata gattttttct
    23641 aactctttat cgcgttcatg tgaaaattca ttatagagtt tcttaaccgc acgattgaat
    23701 ttgaggtttt cttgttcaac ttctttaata tgatccagtc ttctacgtgt ctcaagtgtc
    23761 tgtgcctcta actgttcaat aatacggtta acatctgagt cagaatctgt catctgctta
    23821 gcttgtttaa caataaatgg agctaaacca aggcgagaag caatttcaaa tgcatttgag
    23881 cgtccaggaa ctccttgcat aaagcgatat gtggggctaa gcgtttctgc atcaaattcc
    23941 atgctcgcat tctctacaaa atttgtctca atcccataag cttttaattc tggatagtgc
    24001 gtggtcgcca tcgttttgat atgacttaac ctaaggtgtt ctaaaatagc catggccaaa
    24061 ctagcacctt cttgaggatc cgttcctgct cctagttcat caaagagaac taaactattg
    24121 tggtcagcct cgtttaaaat actgactatg tgcgtcatat gactagaaaa agttgataga
    24181 ctttgttcaa tagattgctc atcgccaata tctgcaaaga tattgttaaa tactgcaatt
    24241 ttactacctt tatccgctaa tactggcaaa ccagactgtc ccattaattg tgctaaaccg
    24301 agtgttttta gcataatcgt cttaccacca gtattgggac cagtgatgac aattgcagtt
    24361 aaatcgtgat caaaatgtaa gtcattagcc acaggattac ttaacagagg atgacgaaca
    24421 ttgattaatg ctaacgtgct gtcattagaa atcttaggta tcgtcgcctt attatcagac
    24481 ataaaaagat atttagccct tacaaaatca agatgcccaa gaatccatgc attatttcta
    24541 atagtggcga catgaggtct taacaagtct gaaaatgcgt gtaaaatacg accttcttcg
    24601 tggcgttcgt cagctctaag ctgcgttatc tcttcgttta gtgtaactac agcacgaggc
    24661 tcaatataaa cagtacttcc tgaagaagag atgtcatgaa ccacaccaga aatacgattc
    24721 cgataagtat tttttactgg taggacactt cgtccactac gactagcgat tagattctct
    24781 gataaaagct ctgctttttc cttaagcata tcctgtaaaa tctgacgaac ccgtcgttca
    24841 ctatttgtta attgacgacg gatacgctct aattctggac tcgcaaaatg ttctaaaaaa
    24901 ccaccatcat tgatagcttg aaaagaccct tgcagattag ggaattgttc caaattttca
    24961 aacaaacgat ccaaagattg gaaagaaaca ttatccaaat cagaataaaa gtgaatcata
    25021 tccgaagaac tctgtaaaac ttttttgata gccaaaagtt cttgaatatt aagatcagtt
    25081 gaaagctcta agcgttttaa actctcagag attgaactta ggctgactat gccaaatgag
    25141 tgatgttcaa caaaaatctg ttccatgtca gaaatttcat taaaactttt ttctatttta
    25201 ggagcctccg tcatcggctc cagctctaat aattcttctt gtgattgttc tgtcttgaga
    25261 taaggtaata gcaattcctt aactttgtta aattctaact gctctaaaat cttgttattc
    25321 atactcttca ttatacctaa aatgatggaa aatggggcaa gaaagtcact aagttcccat
    25381 gcgttctctt tatttctaaa cagtttctcg taaataaaga acctaaaggt gaaactcact
    25441 ccaggttctt atattagtca caaaacgata tcaaataatg gcttgaatcc acaatttttg
    25501 aataattatt gtcaatggtg gaaggtgttc gatcaccaaa cgagtaagaa aactagagtg
    25561 caaatagtgc tgtaaaaaag gcatagggac cgttgcaaaa atagacagta acatgttcaa
    25621 aaaaagaagc gatactagaa acgctagtcc gccactcaaa cattttgtcc actggttatc
    25681 aaaataattt agcaaaagga aatgaacaaa aatcccaaga aagcgactca atgcataccc
    25741 caataagaaa atgataaaaa aggcaaggcc tgcataataa accttatcta aaacaaagat
    25801 atcaactgat ttaaagaaaa agactgatgt tccttctacc gggttagaat aaggaatcca
    25861 aagcgttagc ttatgggcta atccaatata aaatcgatta gctaccaaaa gagaaagtag
    25921 ggctcctaag acataaaacg attgaagaat aatacctcta ctataaccaa tataaaaatt
    25981 ccaagttaaa ataagcacta tcaataaaga taacatgatt aaacctcatt cacatctatt
    26041 tgattagctt tttcttgaag accaaccaag gttttttgac gtaaatctaa aatttcagct
    26101 tccatttttt caatggcaat ctctctgctt aattgtgttg acaaggtatt gatagccatt
    26161 aaaatagcga tagtctcgtc atcagcttct ggtaaatgat ttttaagagc ctgatacttt
    26221 tctttagcta cccgctcaac ttcttccata aagagattgt ctttgtcagt tgtcaatgtt
    26281 aatgtttttt caccaaaggt gaatttatag cgattaatac ttttcatagg gtcacctcat
    26341 tagcattata ccgtaaaatg aaactttcgt aaaatagtca acaccattgc cctaacccgt
    26401 ctaaacattc gagtctctgt tttctttgtt aaataaactt atgaattaac catag
//
LOCUS       DAXWIQ010000023        25374 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000023 DAXWIQ010000000
VERSION     DAXWIQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25374
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.023"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            53..1237
                     /locus_tag="ACGEUI_000994"
     CDS             53..1237
                     /locus_tag="ACGEUI_000994"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFF8165838.1"
                     /translation="MTKEVVITSAYRTPIGNFGGVFKSLSAVDLGVTVVTKILADTGL
                     KSDAIDEVIFGNVLHAGLGQNVARQVALNAGLSYDTPAFTIDMVCGSGLKAVELGAQK
                     IQTGNADIVLVGGTENMSQAPYVLQGQRWGSRMGDSKVVDTMLKDGLSDAFAGYHMGI
                     TAENIVQQYGLTREEQDAFAADSQRKAQLAIEKGRFKEEIAPVTIPQRKGEPLLVDQD
                     EYPKFGTTVDKLAKLRPAFIKDEGTVTAGNASGINDGAAAILLMSKEKAEELGLPILA
                     KITSYASAGVDPSIMGCGPIPATKKALAKAQLTIDDIDLIEANEAFAAQALAVSRDLG
                     FDNEKVNVNGGAIALGHPIGASGARILVTLLAEMAKRDVRHGLATLCIGGGQGQSIIV
                     TR"
     gene            1249..1908
                     /locus_tag="ACGEUI_000995"
     CDS             1249..1908
                     /locus_tag="ACGEUI_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HFF8165839.1"
                     /translation="MKENKRIAIAEAISHIKDGDTIMVGGFMANGTPEALIDALVDKG
                     TKDLTLICNDAGFVDRGVGKMVANHQFKTIYATHIGLNKEAGRQMTAGETTIELIPQG
                     TFAEKIRIGAYGIGGFYTPTGVGTLVAEGKETKTIKGKTYLLEYPFEADVALIFANQA
                     DEMGNLQYSGSENNFNQLMAACAKTTIVQAREIVPVGTIQPECVHTPHIFVDYIVKEG
                     V"
     gene            1911..2558
                     /locus_tag="ACGEUI_000996"
     CDS             1911..2558
                     /locus_tag="ACGEUI_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HFF8165840.1"
                     /translation="MAVALSKEEIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPE
                     EMTVYFQSENGFIGLGPKSDDPNSTIVNAGGQPVTVYPGAAFFNSADSFGIIRGGHVD
                     LTVLGALEIAENGDIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKE
                     CTLPLTAKGVVDLIITEMGVFKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG"
     gene            complement(2680..3360)
                     /locus_tag="ACGEUI_000997"
     CDS             complement(2680..3360)
                     /locus_tag="ACGEUI_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8165841.1"
                     /translation="MDKETLNYWKTVITFLHDVLGDNYEIILHVIDKNDIYIGELVNS
                     HISGRSKQSPLTTFALDLITNKVYKEKDFVTNYKAIVSPQHKEVRGSTFFIKDKKGNL
                     EGMLCINLDISAYQGVARDLLKLVNLNLEHFIPTAKEPKTVTPQPEEAVEILTSNIQD
                     IIGQIIDPSLLRHNVHLSQDVKIDIVAKLYEKGVFQLKGAVSKVADILCISEPSVYRY
                     LKKIEADQ"
     gene            3534..3899
                     /locus_tag="ACGEUI_000998"
     CDS             3534..3899
                     /locus_tag="ACGEUI_000998"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFF8165842.1"
                     /translation="MKSYPEPMGPYSTYTIEGHFLYTAGQLPLNPVTGQLSDGFEAQC
                     RQVFVNLQSILAEQKLDLNHIYKLNVYLTDVTNVEILNHVMTDLFEEPYPVRTAVQVS
                     ALPLQALIEVEAVARVKQL"
     gene            3936..4955
                     /locus_tag="ACGEUI_000999"
     CDS             3936..4955
                     /locus_tag="ACGEUI_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8165843.1"
                     /translation="MDIIIGTSLLILVLAIFSLFNYKAPHGAKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVAIAIGVSPGYAVLIGLSVSGTG
                     IIPGFVAGYVVSFLIKWMEKNIPGGLDLISIIIVGAPLTRFLAQLITPVINSTLLTIG
                     DILTSSANSNPIIMGMILGGTIVVVATAPLSSMALTAMLGLTGIPMAIGALSVFGSSF
                     MNGVLFYRLKLGERKDNIAFAIEPLTQADVTSANPIPIYVTNFVGGAACGVLIALMKL
                     VNDTPGTATPIAGFAVMFAYNPVAKVLITALGCIIISLIVGYIGGSVFKNYRLVTKQE
                     LQARN"
     gene            5410..5730
                     /locus_tag="ACGEUI_001000"
     CDS             5410..5730
                     /locus_tag="ACGEUI_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165844.1"
                     /translation="MNTLLSLMNSIEQEARTIYDSYDAKKKALADKKEEELKQLFLEY
                     EQETQLLLEAKREVFFEANRQEQKASKKRLATQQLALHAILEEHKLTVVRELVDKVVE
                     TYGH"
     gene            5720..7741
                     /locus_tag="ACGEUI_001001"
     CDS             5720..7741
                     /locus_tag="ACGEUI_001001"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFF8165845.1"
                     /translation="MAISQMKKLAMVFEKDYLDLVLKTLQQSQLVEVRDMKQLKHWQD
                     AFNKGNVKLPQIVQYDLTHQKPLLDDEALQYLLQSQQELENGLASLSAFLPPIGKLTA
                     LRQKTPSLSFKQFEERHRQQAAQTALKMMSQKIERLEQLQSKIDQLTEYCQELEKWRS
                     LTVLPQDLAQFHFLSARVGTIPSTANNHFYHQLKQHKGLLKQHKGLFIEEVYHTEFEY
                     GLVLFWQAQDTIHLQKYQFKPLLYKEQLLPSEQLRINKELLTNWLAEKDSLLKELRQS
                     QKILAQLQVEIDYVLSQYQRQQTKKQLLGTRHLIALEGWIEADSVNQLKGLMTKTLGD
                     MFYLDSYDVTPDDWEDVPIKLRNHRYIAPFELVTEMYALPKYQEKDPTPFLAPLYLTF
                     FGMMVADLGYGLLLYAVTLAALVFFNLQKTSKRLVTFFNILAISVAIWGLIYGSFFGF
                     DLPVALLSTKTDVITILVVSLLFGFVTLIFGLLLGAWQQVRMKAYATAYTSSLAWTFI
                     LLGLLLFILGKNVSGLAYLSVIGKWLALGNAFGILVVSLLKSKSLLGLGSGLYNLYGI
                     SSYLSDLVSFTRLMALGLSGASIGAAFNMIVGIFPPVTRFTVGIFIFILLHAINIFLS
                     MLSGYVHGARLIFVEFFGKFYEGGGKAFNPLKLADNYVNVNEETDLEDN"
     gene            7743..8222
                     /locus_tag="ACGEUI_001002"
     CDS             7743..8222
                     /locus_tag="ACGEUI_001002"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFF8165846.1"
                     /translation="MEHLAHYFTAHGGVFFAALGIVLAVALSGMGSAYGVGKGGQAAA
                     ALLKEEPEKFTSALILQLLPGSQGIYGFAIGILIWMKLTPELSVNQGLAYFLVSLPIA
                     IVGYFSAKHQGNVSVAGMQILAKRPKDFMKGVILAAMVETYAILAFVVSFILLNKIG"
     gene            8290..8874
                     /locus_tag="ACGEUI_001003"
     CDS             8290..8874
                     /locus_tag="ACGEUI_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFF8165847.1"
                     /translation="MNDITQLRQNVLEKAHQEGQQCLKIATDSLDTDFKERQQQGLHD
                     LKAKRQKELKALEQQFQVAQQQLKNQERQALLALKQDSIKELFEASLEKMTNFSKEEE
                     LAFLKQVLSKYPEQPLQVTFGEKTGQKFSSYDCAELRLAFPQLSYNQELIPQEAGFLV
                     SLDQVDDNYLYRYLLESVLKEESSRIIDMLFSEI"
     gene            8890..9888
                     /locus_tag="ACGEUI_001004"
     CDS             8890..9888
                     /locus_tag="ACGEUI_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFF8165848.1"
                     /translation="MPTFLELNTTISVKEKELLTKEQFDKLLQAPNTTTLARLLHQSV
                     YHLTVDDLNDLDRLESILMAELTKTYRWAFAETPQPDIVQLFTLRYTYHNVKVLLKAK
                     ASQADLSHLLLPIGDKPLVALEHLIRTMTSDEFPKEVVTEIQSIWAEYQDYQDIRVLE
                     IGTDLAYFKALKQIAQRLEDPVFQQAVLIVIDLYNLITVRRAKSQNKPISFMMQLLSD
                     EASRPSKTFITLEDDKDLMTWFENVTPDSYMTALKPYSEKLRQGTLQTTELEYLVDEC
                     LYHLFAKAKYQVDGPYVLARFLLAKSFEVKNLRLLAAALANDLPKERVIERMRPIA"
     gene            9885..10205
                     /locus_tag="ACGEUI_001005"
     CDS             9885..10205
                     /locus_tag="ACGEUI_001005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFF8165849.1"
                     /translation="MTKTLYKVGVIGNRDVILPFQMIGFQTFPVIKPQDAINQLRQLA
                     MEDFGIIYITEDIAAAIPEVLTHYDNQVLPAVIPLPTHQGAQGIGLSRIQAMVEKAVG
                     QNIL"
     gene            10406..12181
                     /locus_tag="ACGEUI_001006"
     CDS             10406..12181
                     /locus_tag="ACGEUI_001006"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFF8165850.1"
                     /translation="MNQGKIITVSGPLVVASGMQEANIQDICRVGHLGLVGEIIEMRR
                     DQASIQVYEETSGIGPGEPVVTTGCPLSVELGPGLISEMFDGIQRPLDRFQKATDSDF
                     LIRGVAIPSLDRKAKWAFIPKLSVGQEVVAGDILGTVQETAVIEHRIMVPYKVSGTLV
                     AIHAGDFTVTDTVYEIKQEDGSIYQGSLMQTWPVRQSRPVAQKLIPVEPLVTGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQIAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLQEMPGDEGYPAYLGSRIAEYYERAGRVRTLGSQEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYQDDVGSYIDRKQ
                     QSNWSNKVTRAMAILQREASLEEIVRLVGLDSLSEQDRLTMAVARQIREDYLQQNAFD
                     SVDTFTSFPKQEAMLTNILTFNEEASKALSLGAYFNEIMEGTAQVRDRIARSKFIPEE
                     NLEQIKGLTQKVTKEIHHVLAKGGI"
     gene            12182..13597
                     /locus_tag="ACGEUI_001007"
     CDS             12182..13597
                     /locus_tag="ACGEUI_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFF8165851.1"
                     /translation="MSVLKEYRTVSEVVGPLMIVDQVAGVHYNELVDITLHNGERRKG
                     QVLEVQGDKAMVQLFEGSTGINLAKTKVRFTGHPLELAVSEDMVGRIFDGMGQPIDGG
                     PELIPEKYLDIDGQAINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDDNFAVVFAAMGITFEEAEFFMNDLRETGAIDRSVLFINLAND
                     PAIERIATPRIALTTAEYLAYEKGMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLIGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSQE
                     LYKNGFRPPINVLPSLSRLKDKGSGEGKTRQDHAATMNQLFAAYAQGKQAKELAVVLG
                     ESALSETDKLYVAFTNRFEEEYINQGFYTNRSIEESLDLGWELLSILPRTELKRIKDD
                     MLDRYLPKADTTMTKVFVAND"
     gene            13642..14268
                     /locus_tag="ACGEUI_001008"
     CDS             13642..14268
                     /locus_tag="ACGEUI_001008"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFF8165852.1"
                     /translation="MARLNVKPTRMELSNLKNRLKTATRGHKLLKDKRDELMRRFVDL
                     IRENNELRQTIEKELAANMKEFVLAKASENSLMVEELFAVPVHEVTLWIDIENIMSVN
                     VPKFHVQSNTAREQEQGEFAYSYLSSNSEMDNTIQKTKELLEKLLRLAEVEKTCQLMA
                     DDIEKTRRRVNGLEYSIIPQLKETIHYIELKLEEAERASLVRIMKITS"
     gene            complement(14388..15650)
                     /locus_tag="ACGEUI_001009"
     CDS             complement(14388..15650)
                     /locus_tag="ACGEUI_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFF8165853.1"
                     /translation="MADFNFDIDQIADNAVIKTDKTTDIISDLPTDTNGQISFFEKLS
                     ADQQTAITAKAPALVDTFLADQNALLDFGQSAVEGVNATVNHILAEQKKLQIPQVDDL
                     LKSTNRELNGFIAKYKDATPVDLDKKPNFLQKLFKQSRDTLQEFYFDSQNIEQKMDSM
                     AAAVVKQEDTLARNIVSAELLIEDNTKSIEHLVGVIAFIEASQKEASQRAAALQKDLK
                     TKDSATPDYQIKADLLARTTEVINTLEQQHTEYLSRLYVAWATTPQMRNLVKVSSDMR
                     QKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGMTADAIINANNAALQMLAETSKEAIPA
                     LEQSAQNPTLSMKSVTSLAESLVAQNNGIIAAIDHGRKERAQLESAIIRSAETINDSV
                     KLRDQNIVQALLSEGKETQKTIDKITRA"
     gene            complement(15663..16541)
                     /locus_tag="ACGEUI_001010"
     CDS             complement(15663..16541)
                     /locus_tag="ACGEUI_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165854.1"
                     /translation="MIRKYDRTSTKKKSLNWIWLIIAFFMISSFIGGSSFTESLLDIL
                     PAIAIGGTGYAIFRVRSHQKRLAKAKIAKQLEDLKAKIQLADRKVRLLDTYLADHDDF
                     QYNVLAQQLLPQLSDIKAKAITLKDQLDPQIYRRITKKANDVESDITLQLETLQIATT
                     LNPQPLKTPSPNLINKAPELKPYYDNIQTDHQAILAKIQGADNQEELLALHDANMRRF
                     EDILTGYLKIKEEPKNYYNAAARLEQAKQAIQQFDEDLDETLRRLNESDLKDFDISLR
                     IMQGATQRRTTHHQKD"
     gene            16979..18271
                     /locus_tag="ACGEUI_001011"
     CDS             16979..18271
                     /locus_tag="ACGEUI_001011"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFF8165855.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPQKISVIGNGVVVNPKSLVKELAYLHDEGVTTDNLRISDRA
                     HVILPYHIQLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFAERL
                     RINLAEKNRLFEKMYDSTPLGFDAIFEEYYAYGQEIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWQEDITGVRSLDELPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWASI"
     gene            18598..19641
                     /locus_tag="ACGEUI_001012"
     CDS             18598..19641
                     /locus_tag="ACGEUI_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HFF8165856.1"
                     /translation="MNKKVMSLGLVSTALFTLGGCTNNSSKQTTDNSLKIAMITNQTG
                     IDDKSFNQSAWEGLQAWGKENKLEKGKGYDYFQSANESEFTTNLESAVTNGYNLVFGI
                     GFPLHDAVEKVAANNPDNHFAIVDDVIKGQKNVASITFSDHEAAYLAGVAAAKTTKTK
                     QVGFVGGMEGDVVKRFEKGFEAGVKSVDDTIKVRVAYAGSFADAAKGKTIAAAQYAEG
                     ADVIYHAAGGTGAGVFSEAKSINEKRKEEDKVWVIGVDRDQSEDGKYTTKDGKSANFV
                     LTSSIKEVGKALVKVAVKTSEGQFPGGQITTFGLKEGGVSLTTDALTQDTKKAIETAK
                     KAIIEGTITVPEN"
     gene            19814..20353
                     /gene="nusG"
                     /locus_tag="ACGEUI_001013"
     CDS             19814..20353
                     /gene="nusG"
                     /locus_tag="ACGEUI_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFF8165857.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGQTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRAILLSMGQTIDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLML
                     NMFGSETVAEVELYQIAEL"
     gene            20724..22079
                     /gene="nga"
                     /locus_tag="ACGEUI_001014"
     CDS             20724..22079
                     /gene="nga"
                     /locus_tag="ACGEUI_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotine adenine dinucleotide glycohydrolase"
                     /protein_id="HFF8165858.1"
                     /translation="MRNKKVTLAHIVAKTSVAIALAGAMGSSLLANSTTYAVSGKENK
                     KSDVKYETTKVMEANATSSKEDNHVMHTLDGSMSTVWEENSPGGGVGEVLSYKFVSPM
                     RIGRILIVNGDTSSKENYYKKNRIAKADVKYYNGNKLVLFQKIELGDTYTKKPHHIEI
                     DKKLDVDRIDIEVTEVHQGQNKDILALSEVTFGNMERDLFEKKFKEIKDKWVTDKQAD
                     EFIETADKYADKAVQMSAVASRAEYYRMYVSRKYHYKKEFVEKLKQVYKESGASHVTS
                     KKDLMLAFDDAKRKSTIGRQENGLFVTSFAEDMALLFTDQGKLKSADQIENIKGVDSG
                     KYSDGVYQYEYDSELTKNIDKLGYIRTASGDTPGANSLNIPGCQTWSGKHIENSESEL
                     IFPSISVKDLKSKAVLAEIDAKGYFEIIDPTIIAPNGDHKKVTGRFKIKKMQDRMQDR
                     K"
     gene            22084..22569
                     /locus_tag="ACGEUI_001015"
     CDS             22084..22569
                     /locus_tag="ACGEUI_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD glycohydrolase inhibitor"
                     /protein_id="HFF8165859.1"
                     /translation="MYKVPKSLEHYQKMFQKEVTVNDLKKYLIGSDKEYRITRRDSYM
                     GDISDPEVILEYGVYPAFIKGYTQLKANIEEALLEMSNSGQALDIYQAVQTLNAENML
                     LNYYESLPFYLNRQSILANITKALKDAHIREAMVHYKLGEFAHYQDTMLDMVERTIET
                     F"
     gene            22593..24308
                     /gene="slo"
                     /locus_tag="ACGEUI_001016"
     CDS             22593..24308
                     /gene="slo"
                     /locus_tag="ACGEUI_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095889392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin streptolysin O"
                     /protein_id="HFF8165860.1"
                     /translation="MSNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETT
                     TTNEQPKPESSELTTEKAGQKMDDMLNSNDMIKLAPKEMPLESAEKEEKKSEDKKKSE
                     EDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTP
                     VDISIIDSVTDRTYPAALQLANKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVN
                     DPTYANVSTAIDNLVNQWHDNYSGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDG
                     TLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSVTFKELQRKGVSNEA
                     PPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAV
                     VLGGDAAEHNKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRS
                     EYVETTSTEYTSGKINLSHQGAYVAQYEILWDEINYDDKGKEVITKRRWDNNWYSKTS
                     PFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGSTLSPYG
                     SITYK"
     gene            24563..24994
                     /locus_tag="ACGEUI_001017"
     CDS             24563..24994
                     /locus_tag="ACGEUI_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165861.1"
                     /translation="MKQQSYQPLRFVYLLVALFAAMLLIARPVMADGENNDPANAAYY
                     KGKSAGEEAGKTAGKEASWTDLTPTNPTIPEPPSDIRETTNKQHYKDGYTDGYRDGYN
                     EGWKSQHPVLTPVKVIWDLISHWLQQFFPNNQATTTVQSMS"
     gene            complement(25180..>25374)
                     /locus_tag="ACGEUI_001018"
     CDS             complement(25180..>25374)
                     /locus_tag="ACGEUI_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165862.1"
                     /translation="VVSLSLATPENQTVSANDTQVTCVGCPDCGPKWSCCPLTCMRDL
                     LRGWGKSAAYYCTWGYYYSE"
BASE COUNT         7923 a         4502 c         5439 g         7510 t
ORIGIN      
        1 cattatataa taaaaatgta agcgtttcaa taatgaaaaa ggagaagttt ctatgactaa
       61 agaagtggtg atcacatctg cttatcgtac cccgatagga aattttggag gagtctttaa
      121 atctttatca gccgtagatt tgggggtgac agttgttaca aaaattttag ctgataccgg
      181 tcttaaaagt gatgccattg atgaagtgat ctttgggaat gtgttacatg caggtctggg
      241 tcaaaatgtt gcccggcaag tagcgctaaa tgcggggctt tcttatgata cccctgcttt
      301 tacgattgat atggtctgtg gttctggctt aaaagcagtt gagttaggtg ctcaaaagat
      361 tcaaacgggc aacgctgata ttgttttagt tggtggtacc gaaaacatga gtcaagcacc
      421 ttacgtttta caaggccaac gttggggatc tcgtatggga gatagtaaag tagtagatac
      481 catgcttaaa gatgggttga gtgatgcttt tgcaggttat cacatgggta taacagccga
      541 aaatattgtg caacagtatg gactgaccag agaagaacaa gatgcctttg cagctgatag
      601 ccaacgtaaa gcccaacttg ccatagaaaa aggtcgtttc aaagaagaga ttgcacctgt
      661 cactattcct cagcgtaaag gtgaaccttt actcgttgat caagatgaat accctaaatt
      721 tggaacgaca gtggataagt tagcaaagtt acgccctgct tttatcaaag atgaggggac
      781 agtaactgct ggtaatgctt caggaatcaa tgatggagca gcggcaattt tattgatgag
      841 taaagaaaaa gctgaagaat tagggctccc tattttagct aaaatcacta gttatgcaag
      901 tgcaggtgta gacccaagta ttatgggctg cggaccaata cctgctacga aaaaggctct
      961 tgcaaaggct cagctgacaa ttgatgacat tgatttgatt gaagcaaacg aagcttttgc
     1021 tgctcaggct ttagcggttt cacgagacct tggttttgac aatgaaaaag ttaatgtcaa
     1081 tggaggagct attgctttgg gtcacccgat tggagcatca ggtgctcgta ttttagtcac
     1141 cttattagca gaaatggcta agcgtgatgt gagacatggc ttagcaacgc tttgtattgg
     1201 tggtggccaa ggtcaaagta tcatcgtaac aagatagaag gtaaccgtat gaaagagaat
     1261 aaaagaatag caatagcaga agctatcagt catattaaag atggtgacac gattatggtc
     1321 ggtggtttta tggctaatgg aacaccagaa gcgttaattg atgcactggt tgacaaaggc
     1381 acaaaagact taaccttgat ctgtaatgat gctggttttg ttgatcgcgg tgttggaaaa
     1441 atggtggcta atcatcaatt caaaaccatt tatgcaacac atattggatt aaataaagaa
     1501 gctgggcgcc aaatgactgc cggtgaaacg acgattgagc tcattcctca aggaaccttc
     1561 gccgaaaaaa ttcgcatcgg agcttatggc attggtggct tttacacgcc aacaggggta
     1621 ggaaccttag tggcagaagg caaggaaaca aaaaccatca agggaaaaac ctatctctta
     1681 gaatacccat ttgaggcaga tgtcgccttg atatttgcta atcaagctga tgaaatggga
     1741 aatttacagt atagtggttc tgaaaataat tttaatcaat tgatggcagc ttgtgcaaaa
     1801 acaacaattg ttcaggcgcg agaaatagta ccagtcggca ccatacaacc agaatgcgtt
     1861 catacaccac atatttttgt agattacatt gttaaagaag gagtatagtt atggcggttg
     1921 cattaagtaa agaagagatt caaaatcgta ttgcaaagcg tgtggccaaa gaattagaag
     1981 atggtacttt ggtaaattta ggtattggtc taccaaccaa agtagcaaat tttgtgcctg
     2041 aagaaatgac agtgtatttt caatctgaaa atgggtttat tggactaggt cctaagtcag
     2101 atgatccgaa ttcgactatc gttaatgcag gaggacagcc tgtgacggtt tatccaggag
     2161 ccgctttctt taatagtgca gattcttttg gcattatacg tggtggtcat gttgatttga
     2221 ccgtcttggg tgctttagaa attgcagaaa atggagatat cgccaattac ctgattccag
     2281 gcaaaatggt tcctggtatg ggaggagcga tggatttatt agttggtgct aaaaaagtca
     2341 tcgtagctat ggagcatacc aataaaggaa agcataagct cctaaaagaa tgtaccttac
     2401 ctttaacagc aaaaggtgtg gttgacctta ttatcacaga aatgggtgtg tttaaagtaa
     2461 cgccagatgg tatccaagta attgaaatca gtgaagggtt tacttttgat gaggttcaag
     2521 ctgcgacagg tgtacctcta agcatagctg aaggttaatg accagtagcc ttttcttgcc
     2581 ataaaggcat attgagtaaa aaaaacgtca gtcatcggta tatcagagat tgtgatgctt
     2641 atctgaaata cttttaactg gcgttttatg gtgatttggt tattgatcgg cttcaatttt
     2701 tttaaggtag cggtaaacac ttggttcgga aatacataga atatcagcga cttttgagac
     2761 ggctcctttt agctgaaaga cccctttttc ataaagcttg gcaacaatgt ctattttgac
     2821 atcttggcta agatgtacat tgtgtctcag taacgaggga tcaataattt gaccgataat
     2881 atcttggatg ttagaggtta aaatttcaac agcttcttct ggctggggtg tgactgtttt
     2941 aggctcttta gcagttggga tgaaatgttc taaattgaga ttgacaagtt ttaagaggtc
     3001 cctagctact ccttgatagg ctgaaatatc taagttaata catagcattc cctcgagatt
     3061 acccttttta tctttaataa aaaaagtaga tccccgaact tctttgtgct gaggactaac
     3121 gattgctttg taattggtca caaaatcttt ttccttatag actttattag taatcaaatc
     3181 aagagcaaag gtggtgagag gagactgttt agaccgtcca ctgatatgac tattaacgag
     3241 ttctccaata tagatatcat ttttatcaat cacatgtaaa ataatttcat agttatcacc
     3301 taagacatcg tgaagaaatg tgataaccgt tttccagtag tttagcgttt ctttatccat
     3361 gtcaaacttc ctatatctat cttcttattt acaaatatag taatacattt tagactaaaa
     3421 aacaattgcc aatcacctta ataataattt tttatcaaaa agagaattta ttattgactg
     3481 tgaatgcggt ttcctgtaaa atttaactat attatcaatg aggaggttcg tatatgaaga
     3541 gctacccaga accaatgggc ccttattcaa cttatacgat tgaaggacat tttctataca
     3601 ccgcaggcca attacccctt aatccagtga caggtcaatt gtcagatggt tttgaggcac
     3661 agtgtcgcca ggtttttgtt aatttacaat ctattttggc agagcaaaag ttggacttga
     3721 atcatatcta taagctaaat gtgtatttaa cagatgtgac caatgtggaa atattaaatc
     3781 atgtcatgac tgatttgttt gaagagccct accctgttcg gacagcggtt caagtgtcgg
     3841 cgcttccttt gcaagctttg attgaagtcg aagctgtcgc cagagtgaag cagttatagt
     3901 gtcttatgta atgagataga ttggtggaat agattatgga tattattatt ggaacaagtc
     3961 ttttgattct tgttttagct atttttagct tgtttaacta taaggctcct catggtgcca
     4021 aggcaatggg agctttggct tcggcagctt gtgcctcttt tttagttgaa gcctttcaag
     4081 attctttttt tggtaaggtt ttaggctttc agtttctaag tgaagtaggc ggagcaaatg
     4141 gctcattatc aggggtagct gctgctatct tggttgccat tgcaattggt gtgtctccag
     4201 gttatgccgt tttgattggt ttgtcagttt caggaacggg tattatccca ggttttgtgg
     4261 ctgggtatgt ggtatctttt ttaatcaagt ggatggaaaa aaatattcct ggtgggcttg
     4321 atttaatctc gattattatt gtcggtgcac cattaactcg tttcttggcg cagttaatta
     4381 cgccagtgat caatagtact ttattgacta ttggagacat cctgacctca agtgctaata
     4441 gcaaccctat tatcatggga atgatactag gcggtacgat tgttgttgta gcaacagctc
     4501 cgctgtcatc aatggctttg acagccatgc ttggtctaac aggtattccg atggctatag
     4561 gtgccttatc tgtctttggc tcttctttta tgaatggtgt tcttttttac cgattaaaat
     4621 tgggtgaaag aaaagacaat attgcttttg ctattgaacc actaacgcag gcagatgtga
     4681 cgtcagccaa tcccattccg atttatgtga ctaattttgt tggtggtgct gcatgtggtg
     4741 ttttgatcgc cctaatgaaa ttggttaatg atacgccagg gacagctact ccgattgccg
     4801 gttttgcggt gatgtttgcc tataatcctg tcgctaaagt gttgataaca gcccttggat
     4861 gtattattat cagcttaatt gtgggttata ttggaggttc tgtctttaaa aattaccgtt
     4921 tagtaacaaa acaagaatta caagcgagaa actaataaac atctttttga caggactttt
     4981 aacaagatag cagttatact gttatcacat tggcttggct attggctggt aaaggtacct
     5041 taccgtcaac tagatcaagg ttactaaatg cctaaagaag tataagataa cacacgaaag
     5101 acagcaatac aaagctttag agttctgtgt catcttactg ctttgacagg tttttagcta
     5161 acctttttct gaactaagcc attgttttta tggatagaat tgaaagcgct taacttaaag
     5221 atgctgttcg tttaattaag ccattatcct atctcaaaaa agctgttagt agataggtta
     5281 gagatttagc tctagcttat attcactggt tgattaaccc cccaaaagta gcctttcttg
     5341 tagctttgtt tttaagtacc tagtgagtca aagaattagc aatagataaa gataaggagt
     5401 tgatatgtga tgaataccct attgtcacta atgaatagta tcgaacaaga ggctagaaca
     5461 atttatgaca gttacgatgc taagaaaaaa gctctagctg acaaaaaaga agaagagtta
     5521 aagcagctgt tccttgagta cgaacaagaa actcagctac tattagaagc taaacgtgag
     5581 gtgttttttg aagctaatcg tcaagaacaa aaagcttcaa aaaagagatt agcgacgcaa
     5641 cagctagccc ttcatgccat tttagaagag cataagctaa ccgttgttag ggagttagtg
     5701 gataaggtgg tggagacata tggccattag tcaaatgaaa aagctggcaa tggtttttga
     5761 aaaagattat cttgatcttg ttttaaaaac acttcaacaa tctcaattag ttgaagttcg
     5821 tgacatgaag caactaaaac attggcaaga tgcttttaac aaaggcaacg tcaagttgcc
     5881 acaaatcgtc caatacgatc tgacgcatca aaaacctcta ctagatgatg aagctttaca
     5941 atacctttta caaagccaac aagagttaga aaacggcctt gctagcttat cagcgttttt
     6001 accaccaata ggaaagttaa cagccttgag acaaaaaacg ccatctcttt cgtttaaaca
     6061 gttcgaagaa cgccatcgac aacaagcagc tcaaacggct ttgaaaatga tgtcacaaaa
     6121 gatagagcga ctagagcagc tgcaaagcaa aatagaccag ctaacagaat actgtcagga
     6181 gttagaaaag tggcgttcct tgactgtttt gcctcaggac ttagcgcaat ttcatttttt
     6241 gtcggcaaga gttggaacga ttcctagtac agcaaataat catttttatc atcagctaaa
     6301 gcagcacaaa ggtcttttaa agcagcacaa aggtcttttt attgaggaag tataccatac
     6361 agaatttgag tacggccttg tcttattttg gcaagcacaa gatacgattc atttacagaa
     6421 atatcagttt aaacccttat tatataaaga acagttactg ccatcagaac aacttcgtat
     6481 aaacaaagag ttactaacga attggctagc tgaaaaagat agtctactaa aagagcttag
     6541 gcagtctcaa aagatactgg cacagttaca ggttgaaata gactatgtcc ttagtcagta
     6601 tcaacggcag caaactaaaa agcaattact cggtacaaga catctaatag ccttagaagg
     6661 gtggatagaa gctgattcgg ttaaccaatt aaaagggcta atgactaaga cactaggtga
     6721 tatgttttat ctggatagtt acgatgtcac gccagacgat tgggaagatg ttcccattaa
     6781 gctacgtaat caccgctaca ttgcaccgtt tgaattggtt acagaaatgt acgctttgcc
     6841 aaaatatcaa gaaaaagatc cgacaccttt tttagctcct ttatacctta ctttttttgg
     6901 catgatggta gctgatttgg gctatggttt gttgctatat gctgtgacac tagctgcatt
     6961 ggtctttttt aatctccaaa aaaccagtaa acggttagtg accttcttta atattttagc
     7021 tatttcagta gccatatggg ggcttattta cggttctttc tttggttttg acttacctgt
     7081 ggcattatta tcaactaaaa cggatgtgat tactattttg gttgtttctt tgctatttgg
     7141 gtttgttaca cttatatttg ggcttttgtt aggagcatgg caacaagtaa ggatgaaagc
     7201 atatgctacg gcctatacgt caagtctagc ttggactttt attttattag gtctgctgtt
     7261 atttattctt ggtaagaatg tatctggcct tgcttactta tcggttattg gtaagtggct
     7321 tgcattgggc aatgcctttg ggattttggt tgtttcttta ctaaaaagca aaagcctttt
     7381 aggtctaggt agtggattat ataatttata tggcattagc tcttacttaa gtgatttggt
     7441 tagctttact cgtctaatgg cattagggct ttctggagct agtattggag cggcttttaa
     7501 catgattgtt gggatttttc caccagtcac gcgctttaca gtaggtatct ttatttttat
     7561 tcttttgcat gcgatcaata ttttcttatc aatgctttca ggatatgtgc atggtgctcg
     7621 tcttattttc gtagagtttt ttggaaaatt ttatgaaggt ggaggaaaag ctttcaatcc
     7681 tttaaagtta gctgataatt atgtcaatgt caatgaagaa acagatttgg aggacaatta
     7741 acatggaaca tttagcacat tattttacag cacatggtgg cgttttcttc gcagccttag
     7801 gaattgtttt agccgtagct cttagtggca tgggctcagc ttatggtgtc ggtaaaggtg
     7861 gtcaagcggc agctgcgctc ttaaaagaag aacccgaaaa atttacgtct gcgcttatct
     7921 tgcaactgtt acccggtagt caaggaatct acggctttgc tattggtatt ttaatttgga
     7981 tgaaactgac accagaactt tcagttaacc aaggattagc ttactttttg gtatctcttc
     8041 caattgctat tgttggttat ttttcggcca agcaccaagg caatgtttct gtagctggta
     8101 tgcaaatctt ggctaagcgc cctaaagact ttatgaaagg tgtcatttta gcagctatgg
     8161 ttgaaaccta tgctattctt gcctttgtcg tctcctttat tctcttaaat aaaattggat
     8221 aaggtatggt ccttatctag attttaaaaa acggtgacac cactaacact atcaaaaggg
     8281 ggaaattagg tgaatgatat aacacagtta agacaaaacg ttcttgaaaa agcacatcaa
     8341 gaaggtcaac agtgcctaaa gatagcgaca gatagtcttg atactgactt taaagagcgt
     8401 caacagcaag gactacacga tttaaaagca aagcgtcaaa aagagttaaa agctcttgaa
     8461 cagcaatttc aggttgcgca gcagcaactc aaaaatcaag aaagacaagc tctactagca
     8521 ttaaaacaag acagtataaa ggagctattt gaagcttctt tggagaagat gacgaacttt
     8581 tctaaggaag aagaattggc ttttttgaag caagtcttgt ctaaatatcc agaacagcca
     8641 ttgcaagtta cttttggcga aaaaacaggg caaaagttca gttcttatga ttgtgctgag
     8701 ctgagattag catttcctca attaagctat aatcaagagc taattcctca ggaagcagga
     8761 tttttagtca gtttagacca agtcgatgac aattatttat accgttacct tttagaatca
     8821 gttcttaaag aagaaagctc ccgcatcatt gatatgcttt tttcagagat ataggaggtt
     8881 tgactagcga tgccaacatt tctagaatta aacactacta tcagtgtcaa agaaaaagag
     8941 ttattgacta aagagcaatt tgataagcta ttgcaggctc ccaatacaac aaccttagct
     9001 cgactgttgc accagtcagt ctatcaccta actgttgacg atctcaacga tttggatcgg
     9061 ctagaatcta tcttaatggc tgagctaacc aagacctatc gctgggcttt tgcagaaacc
     9121 cctcaacctg acatagtaca attatttacc ttgcgttaca cctatcataa tgtcaaagtg
     9181 ttgttaaaag ctaaagctag tcaagcagat ctgagtcact tattactacc aattggtgac
     9241 aagcctttgg tggctttgga acatttgatt aggacgatga cttctgatga gtttccaaaa
     9301 gaggtggtaa cagaaatcca gtccatttgg gccgaatatc aagattatca agacatacgc
     9361 gtcttagaaa ttggtactga cttagcttat tttaaggcgt tgaaacaaat agcacaaaga
     9421 ttagaggacc ctgttttcca acaagctgtt cttattgtga ttgatttgta taatctgatt
     9481 accgtcagac gggccaaatc gcaaaataaa ccaataagtt tcatgatgca gttattatct
     9541 gatgaagcaa gtagaccaag taaaacattt atcacacttg aagatgataa ggatctgatg
     9601 acgtggtttg aaaatgtaac acctgatagc tatatgacag ccctaaaacc ttattcagag
     9661 aaacttcgcc aaggcactct tcaaacgact gagttagaat accttgttga tgaatgcctt
     9721 taccatttat ttgcgaaggc caaatatcaa gtcgatgggc cttatgtgct tgctcgcttt
     9781 ttgctcgcta aatcgtttga agtaaaaaac ctacggcttt tagcggctgc tttagctaat
     9841 gatttaccca aagaacgtgt catagaaagg atgagaccaa tagcatgacc aaaacattgt
     9901 ataaagtcgg ggtaattggt aacagagatg ttattctacc ttttcagatg attgggtttc
     9961 aaacttttcc agtaataaaa ccacaagacg ctattaacca gttgcgtcaa ctagcaatgg
    10021 aagactttgg tattatttat atcacagaag atattgctgc agctattcca gaagtcctca
    10081 ctcactatga caatcaagtt ctaccagcag tcataccatt accaactcat caaggagcgc
    10141 aagggatagg cctgtcacgc atacaagcca tggttgaaaa ggctgtcggt caaaatattt
    10201 tataacacta agacgttagg caaagaggac ttatactctg tttgaggctt atgagtagct
    10261 aactgatgaa atgcaaggac aggaaaattg gtgaagttag cagctgaacg cataggatag
    10321 tttgctgact attcaaaata gtcacattaa accttgacag tatagattag ttagtatgat
    10381 ttggaaaaaa taaaggagga aaaaggtgaa ccaaggaaaa ataataaccg tttcgggacc
    10441 tcttgttgtg gcttctggga tgcaagaagc taatattcaa gatatttgtc gtgtgggaca
    10501 tcttggctta gtcggagaaa ttattgaaat gcgtcgcgat caagcgtcta ttcaggttta
    10561 tgaggaaaca tcagggatcg gtccaggaga accagtagtg actactggtt gtcctttgtc
    10621 ggtcgagtta ggcccgggcc tgatttcaga aatgtttgac ggtattcagc gaccgcttga
    10681 tcgttttcaa aaagcaacgg acagcgactt tttaatccgt ggtgtggcta tcccaagtct
    10741 tgatcgaaag gctaagtggg catttattcc caagctaagt gttggtcaag aagtagttgc
    10801 aggtgatatt ttaggaactg tgcaagaaac agctgtcatt gagcaccgta tcatggttcc
    10861 ttataaagtt tcagggacct tggtggctat tcatgcaggg gacttcacag taacagatac
    10921 agtttatgaa attaagcagg aagacggttc catttaccaa ggtagcctca tgcagacttg
    10981 gccagttcgt caaagtcgcc ctgttgctca aaagcttatc ccagtcgaac ctttggttac
    11041 aggtcaacgg gttattgaca cctttttccc tgttacaaaa ggtggtgccg ctgccgttcc
    11101 tggaccattt ggggcaggaa aaacagttgt gcagcatcaa atagctaaat ttgccaacgt
    11161 tgatattgtt atttatgtcg gttgtgggga acgcggcaac gagatgaccg acgttttgaa
    11221 tgagtttcca gagttaattg acccaaatac aggccagtcc attatggagc gcacggtgtt
    11281 aattgcaaac acctctaata tgccagtagc agcgcgtgaa gcgtcgattt acacaggtat
    11341 taccattgcc gaatatttcc gtgatatggg ctattctgtg gctatcatgg cagactcgac
    11401 atcacgttgg gcagaagctc tgcgcgagat gtcaggacgc ctacaagaaa tgcctggtga
    11461 tgaaggctac ccggcttact tagggagtcg tattgccgaa tattatgaac gggctggtcg
    11521 tgttcggacc ttgggaagtc aagaacgtga gggaaccatt acagccatcg gcgcggtttc
    11581 tcctcctgga ggggatattt cagagcctgt cactcaaaac acccttcgga ttgtcaaagt
    11641 tttctggggg ctcgacgcgc ctcttgcgca acggcgtcac ttcccagcga ttaactggct
    11701 gacgtcttat tcattgtatc aagatgatgt aggaagctat attgaccgta aacagcaatc
    11761 taattggtcc aacaaggtaa ctcgtgccat ggctattttg cagcgtgaag ccagtctaga
    11821 agaaattgta cgcttggtgg ggcttgattc actgtctgaa caagatcgtt tgaccatggc
    11881 tgttgcccgg caaattcggg aggattatct ccagcaaaat gcctttgatt cggtggatac
    11941 ctttacttcc tttccgaaac aagaggccat gctaaccaat attttgacct ttaatgagga
    12001 agccagcaaa gccctttctt tgggagctta ttttaatgag attatggaag gcactgctca
    12061 ggtacgcgat cgcatcgcac gcagcaaatt tatcccagaa gaaaacttag agcagattaa
    12121 agggcttact cagaaggtta ccaaagagat tcaccacgtt ttagcaaagg gaggaattta
    12181 gatgagcgtt ctcaaagaat atcggacggt tagcgaagtg gttggccctt tgatgattgt
    12241 tgatcaagtt gcaggagtgc actacaacga attggtagat attactttgc ataatggaga
    12301 aaggcgtaaa ggtcaagtct tagaagtcca aggtgacaag gctatggtac agctatttga
    12361 aggatcaact ggcatcaacc tagccaaaac caaggttcga tttacagggc atcctttgga
    12421 attagccgta tcagaagata tggtagggcg catctttgat ggaatggggc agcccataga
    12481 cggcggtcca gaacttattc ccgaaaaata ccttgatatt gatggtcaag cgatcaatcc
    12541 tgtcgctcgt gattacccag acgaatttat ccagacaggt atctcagcta ttgatcacct
    12601 caataccctc gttcgtggtc aaaagttacc agttttttcg ggctcagggt tacctcataa
    12661 tgaattggcc gcacagattg ctcgtcaggc aactgttcta aactctgatg ataactttgc
    12721 ggttgtgttt gcagctatgg gtattacttt tgaagaggca gaattcttta tgaatgactt
    12781 gcgtgaaacg ggtgccattg atcgatctgt tttatttatc aatttagcta atgaccctgc
    12841 tattgaacgc attgcaacac cacgtatagc tctgacaaca gccgaatact tggcttatga
    12901 aaaaggtatg catgttttag tcatcatgac agatatgaca aattattgtg aagccttacg
    12961 agaagtatca gccgctcgca gagaagttcc aggcagacga ggttatcctg gctatctcta
    13021 tactaatctc tccaccttat atgaacgggc tggtcgcttg atcgggaaaa aagggtcagt
    13081 gactcaaatt cctattttaa ccatgccaga agatgacatt acccacccta tccccgattt
    13141 gacaggttat attaccgaag gtcagattat tttgtcgcaa gaactatata aaaatggttt
    13201 tagaccgcct attaatgtct tgccatcgct atctcgccta aaagataaag gctcaggtga
    13261 aggtaagact cgacaagacc atgctgctac aatgaatcag ctctttgcag cctatgccca
    13321 aggaaaacaa gctaaggaat tggctgttgt gctcggtgaa tcagcccttt ctgaaacgga
    13381 taaactatat gtggccttta ccaaccggtt tgaagaagaa tacattaacc aaggatttta
    13441 caccaaccgt agcattgaag aaagtcttga cttgggttgg gaattgttgt cgattttgcc
    13501 acgtaccgaa ttaaaacgca ttaaagatga tatgttagat cgttatttgc ctaaagcaga
    13561 taccacgatg acaaaggttt ttgttgcaaa tgattgagtt gaccatgcaa tttcaagccc
    13621 ttgacgaaag gagttatatc aatggcacga ttaaatgtta aaccaacacg catggaactt
    13681 agtaacttga aaaatcgctt gaagacagct acaagaggtc ataaattatt aaaggacaag
    13741 cgggatgagt tgatgcgccg cttcgttgat ttaattcgtg aaaacaatga gcttcggcaa
    13801 actatcgaaa aagaacttgc tgccaatatg aaggagtttg tcttggcaaa agcatccgaa
    13861 aatagtctaa tggtagaaga actctttgct gttccagtcc atgaagtgac cttatggatt
    13921 gatattgaaa atatcatgag tgtcaatgtg cctaaattcc atgtccagtc taatacggca
    13981 agagagcaag aacaaggtga gtttgcttat agttacctca gctcaaatag tgagatggac
    14041 aatactatcc aaaagacaaa agaactatta gaaaagctct tacggttagc tgaagttgaa
    14101 aaaacttgtc aactaatggc tgatgacatt gaaaagacta gacgtcgcgt caatggctta
    14161 gagtattcta ttatcccgca actgaaagag acgattcact acattgagtt gaaattagaa
    14221 gaggccgagc gtgcgagcct tgttcgtatt atgaaaataa catcataaaa gataagtgtt
    14281 atatcaaaca aggcgattat tcgttaagta gtaaaaaagc aagccatcaa agggattaac
    14341 ctaactgatg gcttgctttt taagtagcgt gacaggtgat gtttagctta tgcgcgagtt
    14401 attttatcaa ttgttttttg agtttccttg ccttcactaa gaagtgcttg cacaatattt
    14461 tggtcacgta gtttgacaga atcgttaatg gtttcggcag acctgataat agcactttct
    14521 agttgtgctc gttctttgcg tccatgatca atcgccgcaa tgatcccatt attttgtgct
    14581 actaaacttt ctgccagact tgtcacagat ttcatagaca aagtagggtt ttgtgctgat
    14641 tgttccaatg ctgggatggc ttctttactt gtttctgcta gcatctgaag ggcagcatta
    14701 ttagcattaa taatggcatc agcagtcatt ccagatttaa ccgattgttg catcatgcct
    14761 aactgagcaa tagatagttt catcgttgga atggtattgc ggcgtaacat gcctagtttt
    14821 tgacgcatgt cagaagagac cttgactaga ttacgcattt gcggagttgt cgcccaagca
    14881 acatagaggc gactcaagta ttcggtgtgt tgttgttcga gagtgttgat gacttcggta
    14941 gttcttgcca aaagatcagc ttttatttga tagtctggtg tagcactgtc tttggttttt
    15001 aggtcttttt gtaaagccgc agcgcgctga ctagcttctt tttgactagc ttcgataaaa
    15061 gcaatgacgc ctaccaaatg ttcaatggac ttagtattat cttcaattaa caattctgct
    15121 gagacaatat tgcgagctaa ggtatcttct tgcttgacaa ccgctgcagc catactatcc
    15181 attttttgct caatgttttg agaatcaaaa taaaactctt gcagggtatc acggctttgt
    15241 ttaaagagtt tttgaagaaa gtttggcttt ttatcaaggt ccacaggtgt ggcatccttg
    15301 tatttagcga taaaaccatt gagttcacga ttggttgatt ttaaaagatc atccacttgt
    15361 ggaatctgta attttttttg ctctgccaag atatgattaa ccgttgcatt gaccccttca
    15421 acagcagact gaccaaaatc aagtagcgca ttttggtctg ccaaaaaggt atctaccaat
    15481 gccggggctt tggcagtaat agctgtctgc tggtcagcac ttagtttctc aaaaaaagag
    15541 atttggccat ttgtgtcagt tggtaagtct gagataatat cggtggtttt atcggttttg
    15601 ataacagcat tgtcagcaat ttggtcaatg tcaaaattaa aatctgccat aaagggactc
    15661 ctttagtctt tttggtgatg cgttgttcgt ctttgagtag caccttgcat gattcgaagg
    15721 ctaatatcaa aatctttaag atcactttca ttgagtcgac gaagggtttc gtcaagatct
    15781 tcatcaaatt gttggatagc ttgtttggct tgttctaaac gagcagcagc gttatagtaa
    15841 tttttaggct cttctttgat ttttaagtag cctgttaaga tatcctcaaa cctcctcatg
    15901 ttagcatcat gcagcgcaag caattcttct tgattgtcgg ctccttggat tttagcaagg
    15961 atagcttgat gatctgtctg aatattatca taatacggtt tgagctcagg tgctttgtta
    16021 ataagattag gagatggggt tttcaaaggc tgagggttaa gagttgtggc aatctgcagt
    16081 gtttcgagtt gaagggtaat atcactttca acgtcattgg cttttttggt gatacgacga
    16141 taaatttggg gatctaactg gtcttttaac gtaatagctt ttgctttgat gtcactaagt
    16201 tgtggtaata attgctgagc tagtacgtta tattggaagt catcatggtc tgccagataa
    16261 gtgtctaaca gccgaacttt acgatcagct agctgaattt ttgcttttag atcttctaat
    16321 tgtttggcaa tcttggcctt tgctagccgc ttttgatgag atctaacacg aaaaatagca
    16381 taacctgtac caccaatggc tatggcaggc aaaatgtcta gaagtgactc agtaaaagaa
    16441 gagccaccta taaagcttga aatcataaaa aaagcaatga ttagccaaat ccagtttagt
    16501 gatttctttt ttgtgctcgt gcggtcatac tttctaatca tgtgatattt cctcgatatg
    16561 tttttactgg ctatctcata gaggtaactt actattattt taccttaaaa ccgctatttc
    16621 tgaaagaaaa gagctaatgt tttaagagaa tcgtttaacc ctgctaaagc ttaggagaaa
    16681 cgaggttaaa agcttttctc ttataatgtt cgttttttta ggattgttta tcatggttaa
    16741 aaaaagagtt tctctttttc cttaataatg ctaagaaatc tagcagaaaa aatcattttt
    16801 tgctataata agttgtaaga tatatgctag caggtgagat tcctgcctgt tttctttaaa
    16861 tatcaagagc tacattgctg atcttacaga tctgaccttc tttttggttt ttaagcagtg
    16921 gttagcttgc ctatgatttt tattgagaat aagtgtttca gaaagttagg acaatgatat
    16981 gacatctgta gttgttgttg gtacccagtg gggtgacgaa ggtaaaggta aaatcactga
    17041 tttcttgtca gcggatgcag aagtaattgc tcgctatcaa ggtggtgata atgctggtca
    17101 tacgatcgtg attgatggta agaagtttaa acttcacttg atcccatcag gaattttctt
    17161 cccgcaaaaa atttccgtta ttggtaatgg cgttgtggtc aaccccaaat ctttggtcaa
    17221 agagttggcg tacctgcatg atgaaggagt gacgacagat aaccttcgca tttcagaccg
    17281 agctcatgtt attttacctt atcatatcca gctggatcaa ctgcaagaag atgcaaaagg
    17341 tgacaataaa atcggaacaa ctattaaagg gattggtcca gcttatatgg ataaagctgc
    17401 tcgagttggt atccgtatcg cagatttact ggataaggat atctttgcag agcgcctacg
    17461 cattaattta gctgagaaaa accgtttatt tgaaaaaatg tatgacagta cacctcttgg
    17521 ttttgatgct atctttgagg agtactatgc ttacggtcaa gaaatcaagc aatatgtgac
    17581 agatacttct gtgattttaa atgatgcact tgatgctggt aaacgtgttc tttttgaagg
    17641 tgctcaaggg gttatgcttg atattgacca aggaacttac ccatttgtaa cgtcttcaaa
    17701 cccagttgct ggtggtgtaa ccattggttc tggtgttggc ccaagtaaaa tcaacaaagt
    17761 agttggtgtc tgtaaagcct acacaagccg tgtcggtgat gggccattcc ctacagaact
    17821 ctttgatgaa gtgggtgagc gcattcgtga agtgggtcat gagtacggga caacgaccgg
    17881 ccgtccacgt cgtgtcggtt ggtttgactc agttgttatg cgccatagtc gtcgtgtgtc
    17941 agggattact aacctctctc tgaattcaat tgatgttctt tcagggcttg atacggttaa
    18001 gatttgtgtg gcttatgacc ttgatgggaa acgtattgac tattacccag caagccttga
    18061 acaactcaaa cgttgcaaac caatctatga agaattacca ggctggcaag aggacatcac
    18121 aggtgttcgt agccttgatg agcttcctga aaatgcccgc aactacgttc gtcgtgttgg
    18181 agaattggtt ggcgttcgca tttcaacctt ctcagttggt ccagggcgtg aacagaccaa
    18241 tattcttgag tcagtctggg catctatcta aggtgtctag tatgacaaag agttggatag
    18301 cgtttccagc tctttttacc gcttgttgga ttgtatagtt agtcatgcta ttactagtag
    18361 cttgagtttt gtcgttgtct tatctcctta agaggtgatt tttgaggaga taacgggttt
    18421 taccagttga tctcactttc tcgttcggtc attttggtga aaaagagatt tttcagcaaa
    18481 aatagtagga taaaactgat ttttttgata tactaaactt ttcgttctag agcataagta
    18541 gaacgttaac aatgacacat cactttagga ttaaacattt aggaggttca ttttcacatg
    18601 aacaaaaaag taatgtcact tggtcttgtt tcgactgccc tattcacatt aggaggctgc
    18661 accaataact cctctaaaca aacaactgac aattcattaa aaatcgctat gatcactaat
    18721 cagacgggta ttgatgacaa gtcatttaac cagtcagcct gggaaggctt acaagcttgg
    18781 ggaaaagaaa ataaacttga aaaaggaaaa ggctatgatt atttccaatc agccaatgaa
    18841 tcagagttta ccacaaacct tgagtcagca gtaaccaatg gttataatct tgtttttggg
    18901 attggatttc cattacatga cgctgtagaa aaagtagccg caaacaatcc tgacaaccat
    18961 tttgcaattg tggatgatgt gattaaaggt caaaaaaatg ttgcaagtat caccttttca
    19021 gaccatgaag cggcatacct agccggtgtt gcagcagcta aaacgacaaa aaccaagcaa
    19081 gttggttttg taggtggtat ggaaggagat gttgtcaagc gctttgaaaa aggttttgaa
    19141 gctggtgtga aatcagtaga tgataccatc aaagtaagag ttgcttatgc aggctctttt
    19201 gcagatgctg ccaaaggcaa gacgattgca gctgctcaat atgctgaagg cgcagatgtt
    19261 atttatcatg cagcaggagg cacaggggcg ggtgtcttta gcgaagctaa gtctatcaac
    19321 gaaaaacgta aagaagaaga taaggtttgg gttattggtg ttgaccgtga ccaaagtgaa
    19381 gatggaaaat acaccacaaa agatggcaag tcagctaatt ttgttttgac ctcaagtatc
    19441 aaggaagtcg gaaaagcttt agtaaaagta gccgtaaaaa cctcagaagg ccaattccca
    19501 ggtggtcaaa taaccacttt tggtttaaaa gaaggtggtg ttagccttac aacggatgct
    19561 ctgacacaag acactaaaaa agctattgag actgctaaaa aagcgattat cgaaggaacc
    19621 atcacagttc ctgaaaacta aaactgtaca ataccgtcca gagtcgtcac gaaccgactt
    19681 tggacttttt aataaaggtg tcaagtagct ccacagatac gttgacacca gccctgtctt
    19741 ttattataat gaaggtgata gaaaacagcg caaaagccaa aaaaagggct tttttataat
    19801 gaaaggaaac atcatgctag attcatttga taagggctgg ttcgtcctcc aaacttactc
    19861 aggctacgaa aacaaagtaa aagaaaacct cttgcaacgt gcccaaacct ataacatgtt
    19921 ggacaatatt ttgcgcgtcg aaattccgac acaaacggtt aacgttgaaa aaaatgggca
    19981 aaccaaagaa atcgaagaaa atcgctttcc agggtatgtc ttggttgaaa tggtcatgac
    20041 agatgaagct tggtttgttg tgcgtaacac accaaacgtt acaggttttg tgggatcaca
    20101 cgggaaccga tcaaaaccaa caccacttct tgaagaagaa atccgtgcta tcttgttgtc
    20161 tatgggtcaa accattgatg tctttgatac caatatcaaa gaaggtgatg tggttcagat
    20221 catagacggt gcctttatgg gacaagaagg tcgtgtggta gaaatcgaaa acaacaaagt
    20281 gaagctgatg ctcaacatgt ttggctctga aacagtagca gaagttgaac tttatcaaat
    20341 tgcagaacta taggctctca aaggtctgat ggtataagta ttaccatcag acctttttta
    20401 gttggcagtg aagcgtgttt acaaaccaat ggatgactat tgttaacatc attgtaatca
    20461 gaaataatta acagatctat tactgataac ggtgctacta tcaaatattt aactagtact
    20521 tttaatctgg tttaaataga acagatgtga aggttctgtg ataataagat ggaaatatcc
    20581 ccttgaaact tgtaacagtt ggctacttgt ttttttgtgt tcattttttt gatagaaaag
    20641 cctaaaacga tgttaataaa tcgcattgac aaaaactagt tgttaatgta tagtttactt
    20701 aaaaataata taaggtggtt tacatgagaa acaaaaaagt aacattagct catattgtcg
    20761 caaagacaag tgttgctatt gctttggctg gagcaatggg tagcagttta ttagctaata
    20821 gcacaacgta cgctgttagt ggcaaagaaa ataaaaaaag cgatgtcaaa tatgaaacga
    20881 ccaaagttat ggaagctaac gcaacttcct ctaaagaaga caatcatgtc atgcacacat
    20941 tagacggctc aatgagtact gtctgggagg aaaattcacc tggtggtggc gttggtgagg
    21001 tactttccta caagtttgtt tccccgatgc gtattgggag aattttaatt gtgaatggag
    21061 atacatctag caaggagaat tactacaaga aaaatagaat tgcaaaggct gatgttaaat
    21121 actataacgg gaataaattg gtcctctttc aaaaaattga attaggcgac acctacacta
    21181 aaaaaccgca tcacattgag attgataaaa aattagatgt tgatcgtatt gatattgagg
    21241 taacagaggt ccatcaggga caaaacaagg atattttagc cttgtcagag gtcacttttg
    21301 gcaatatgga acgcgatctt tttgaaaaaa agtttaaaga aattaaagat aaatgggtaa
    21361 cagataaaca agcagatgag tttattgaaa ctgccgacaa gtatgctgat aaagctgttc
    21421 agatgtctgc tgttgcgtca cgtgctgagt attaccggat gtacgttagc cgcaaatacc
    21481 actacaaaaa agagtttgtt gaaaaactaa aacaagtcta caaagaaagc ggagcttctc
    21541 acgttacaag taaaaaagat ttgatgttag cttttgatga tgctaaaaga aagtcaacga
    21601 ttggtcgaca agaaaacggt ctttttgtga caagttttgc tgaagatatg gcattgctct
    21661 ttactgatca aggtaagtta aaatcagctg accaaattga aaatataaaa ggtgtcgata
    21721 gcggaaaata tagcgatgga gtttatcaat atgagtacga ttctgaacta acaaaaaaca
    21781 ttgataagct aggctatatc cgaacagcta gcggagatac ccctggagca aattcgctca
    21841 acattcctgg ttgccaaacg tggtcaggaa aacacattga aaattcagaa agtgagttaa
    21901 ttttcccatc gattagtgtt aaagatctaa aatctaaagc tgtcttagca gagattgatg
    21961 ccaaaggcta ttttgaaatt attgatccta ccatcattgc tccaaatggt gaccataaaa
    22021 aagtaactgg tcgcttcaaa attaagaaaa tgcaagatag aatgcaagat aggaagtaac
    22081 aatatgtata aggtgccaaa gagtttagaa cattaccaaa aaatgtttca aaaagaagta
    22141 actgttaatg atttaaaaaa atacttgata ggtagtgaca aagagtatcg tatcactaga
    22201 agagactcct atatgggaga tatttctgat ccagaagtta ttttagagta tggggtttac
    22261 cctgctttca taaaaggcta tacccaattg aaagctaaca tcgaagaagc attattagaa
    22321 atgtcaaata gcggtcaagc attagacatt taccaagcgg ttcaaaccct aaacgctgaa
    22381 aacatgttat taaattatta cgaaagcttg ccattttatt taaaccgtca aagcatacta
    22441 gctaatataa ccaaagcgtt aaaagatgcg catattagag aggctatggt acattacaaa
    22501 ttaggagaat ttgctcacta tcaagatact atgcttgata tggtcgaaag aacaatagaa
    22561 acattttaga atgataaaaa ggtatgaagg acatgtctaa caaaaaaaca tttaaaaaat
    22621 acagtcgcgt cgctgggcta ctaacggcag ctcttatcat tggtaatctt gttactgcta
    22681 atgctgaatc gaacaaacaa aacactgcta gtacagaaac cacaacgaca aatgagcaac
    22741 caaagccaga aagtagtgag ctaactaccg aaaaagcagg tcagaaaatg gatgatatgc
    22801 ttaactctaa cgatatgatt aagcttgctc ccaaagaaat gccactagaa tctgcagaaa
    22861 aagaagaaaa aaagtcagaa gacaaaaaaa agagcgaaga agatcacact gaagaaatca
    22921 atgacaagat ttattcacta aattataatg agcttgaagt acttgctaaa aatggtgaaa
    22981 ccattgaaaa ttttgttcct aaagaaggcg ttaagaaagc tgataaattt attgtcattg
    23041 aaagaaagaa aaaaaatatc aacactacac cagtcgatat ttccatcatt gactctgtca
    23101 ctgataggac ctatccagca gcccttcagc tggctaataa aggttttacc gaaaacaaac
    23161 cagacgcggt agtcaccaag cgaaacccac aaaaaatcca tattgattta ccaggtatgg
    23221 gagacaaagc aacggttgag gtcaatgacc ctacctatgc caatgtttca acagctattg
    23281 ataatcttgt taaccaatgg catgataatt attctggtgg taatacgctt cctgccagaa
    23341 cacaatatac tgaatcaatg gtatattcta aatcacagat tgaagcagct ctaaatgtta
    23401 atagtaaaat cttagatggt actttaggca ttgatttcaa gtcgatttca aaaggtgaaa
    23461 agaaggtgat gattgcagca tacaagcaaa ttttttacac cgtatcagca aaccttccta
    23521 ataatcctgc ggatgtgttt gataaatcag tgacctttaa agagttgcaa cgaaaaggtg
    23581 tcagcaatga agccccgcca ctctttgtga gtaacgtagc ttatggtcga actgtttttg
    23641 tcaaactaga aacaagttct aaaagtaatg atgttgaagc ggcctttagt gcagctctaa
    23701 aaggaacaga tgttaaaact aatggaaaat actctgatat tttagaaaat agttcattta
    23761 cagctgtcgt tttaggagga gatgctgcag agcacaataa ggtagtcaca aaagactttg
    23821 atgttattag aaacgttatc aaagataatg ctaccttcag tagaaaaaac ccagcttatc
    23881 ctatttcata caccagtgtt ttccttaaaa ataataaaat tgcgggtgtc aataacagaa
    23941 gtgaatacgt tgaaacaaca tctaccgagt acacgagtgg aaaaattaac ctgtctcatc
    24001 aaggtgccta tgttgctcaa tatgaaatcc tttgggatga aatcaattat gatgacaaag
    24061 gaaaagaagt gattacaaaa cgacgttggg acaacaactg gtatagtaag acatcaccat
    24121 ttagcacagt tatcccacta ggagctaatt cacgaaatat ccgtatcatg gctagagagt
    24181 gcaccggctt agcttgggaa tggtggcgaa aagtgatcga cgaaagagat gtgaaactgt
    24241 ctaaagaaat caatgtcaac atctcaggat caaccttgag cccatatggt tcgattactt
    24301 ataagtagga ctggttcaag aggttcgtca agcaccttga tgctgcttat ctcttgaggc
    24361 cttgtgccta atcctctcac aaatacagaa gaaagcttgt ctatgagata ccttcataga
    24421 caagcttttt tgatatacta atcaaaaaat tattcttttc tcacatcgtg accttgaagc
    24481 taagctgctt ctaatctata atactaacaa gctgcttttt aacaagcaaa gcataacata
    24541 aaagggagag ataactatta tcatgaaaca acaatcttac cagcctctac gcttcgtcta
    24601 cctcttggtg gctctatttg ctgctatgtt gcttatagca agacctgtta tggcagatgg
    24661 ggagaataat gacccagcta atgcagcata ttataaaggg aaaagcgctg gagaagaagc
    24721 agggaaaaca gctggaaaag aagctagttg gactgatttg accccaacta atccaaccat
    24781 tccagaacca cctagtgata tcagagagac tactaataaa cagcattata aagacgggta
    24841 tacagatggg tacagagatg gttataatga aggctggaaa tctcagcatc ccgtcttgac
    24901 tccggtcaag gttatatggg atttgatctc tcattggcta cagcaattct tccccaataa
    24961 ccaggcaact acaacagtac aaagcatgtc ataataaaaa tgaattaaaa acagccttta
    25021 actgctaatg tgaaaggttg tttttttagg atatgggaat gaacaataaa aaaagagttt
    25081 gtgaaaaagc ctctcggcca cacaaaaaaa caatggcgtc aaagccattg tttttttagc
    25141 ataatcaaga gactactgtc tcagactctc aagtaatttt tactcagagt agtaatatcc
    25201 ccaagtgcaa taatatgctg ctgacttgcc ccaacctctt aacaaatcgc gcatacaggt
    25261 caatggacaa caagaccatt ttggaccaca atctggacat cctacacaag taacttgcgt
    25321 gtcatttgct gaaacagttt ggttttctgg tgttgcaagg ctaaggctta cgac
//
LOCUS       DAXWIQ010000024        24654 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000024 DAXWIQ010000000
VERSION     DAXWIQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24654
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.024"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            50..988
                     /gene="comGA"
                     /locus_tag="ACGEUI_001019"
     CDS             50..988
                     /gene="comGA"
                     /locus_tag="ACGEUI_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFF8165863.1"
                     /translation="MVQALAKAILAKAEQVHAQDIYILPRADQYDLFLRIGDERRLVD
                     VYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYKLSKDKQLSLRLSSVGDYRGQES
                     LVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISNYHQEA
                     QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAV
                     IRASLTGAMVFSTVHAKSISGVYARLLELGVTKAELSNCLALIAYQRLLNGGALIDST
                     QNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII"
     gene            924..1958
                     /gene="comGB"
                     /locus_tag="ACGEUI_001020"
     CDS             924..1958
                     /gene="comGB"
                     /locus_tag="ACGEUI_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFF8165864.1"
                     /translation="MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAE
                     VIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGLGFSDAILTQISLADRHGNIETT
                     LVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
                     HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREW
                     GTLIGQGLDLMTILDIMAIEKSSLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSL
                     MIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLVVAVTIVMIYAAILL
                     PIYQNMGGIF"
     gene            1960..2286
                     /gene="comGC"
                     /locus_tag="ACGEUI_001021"
     CDS             1960..2286
                     /gene="comGC"
                     /locus_tag="ACGEUI_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFF8165865.1"
                     /translation="MINQWNNLRHKKLKGFTLLEMLLVILVISVLMLLFVPNLSKQKD
                     RVTETGNAAVVKLVENQAELYELSQGSKPSLSQLKADGSITEKQEKAYQDYYDKHKNE
                     KARLSN"
     gene            2261..2689
                     /gene="comGD"
                     /locus_tag="ACGEUI_001022"
     CDS             2261..2689
                     /gene="comGD"
                     /locus_tag="ACGEUI_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFF8165866.1"
                     /translation="MKKPVLAIKAFTLLETLLSLSVMSFIILGLSVPVTKSYQKVEEH
                     LFFSHFEHLYRHQQKLAILQQKQRVLDISSTKIVTEGNSLTVPKSITVNHPYRLVIDQ
                     MGGNHSLAKIIFDMTDRRFKYQFYLGSGNYQKTSQSLHSP"
     gene            2646..2930
                     /gene="comGE"
                     /locus_tag="ACGEUI_001023"
     CDS             2646..2930
                     /gene="comGE"
                     /locus_tag="ACGEUI_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGE"
                     /protein_id="HFF8165867.1"
                     /translation="MGIIKKQVKAYIALESMIATGILFSIVILVLSSLQQSQAALTYY
                     RKQQEKLNLALMAVQTRTKEMTLNGCHITILRTDRYISIHDDEGEVMKIE"
     gene            2923..3357
                     /gene="comGF"
                     /locus_tag="ACGEUI_001024"
     CDS             2923..3357
                     /gene="comGF"
                     /locus_tag="ACGEUI_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFF8165868.1"
                     /translation="MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTRTLLKHSHYL
                     ARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEKGKKVLAFGQFKSHDFRKSASNG
                     KGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD"
     gene            3341..3667
                     /gene="comGG"
                     /locus_tag="ACGEUI_001025"
     CDS             3341..3667
                     /gene="comGG"
                     /locus_tag="ACGEUI_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFF8165869.1"
                     /translation="MPFKTNLKAGILLYAMFMASIFLLVLQVYLSQVTALHKEYQAQT
                     DYVKARLIAEIVYQDHRYKASNPVFFKGGQVICRERKERWMLIVKLDQQRQYQFEYLK
                     PKQYSQ"
     gene            3765..4718
                     /locus_tag="ACGEUI_001026"
     CDS             3765..4718
                     /locus_tag="ACGEUI_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8165870.1"
                     /translation="MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEG
                     ASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQTEQLQANHQFTPDAIGFILLYLL
                     EQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
                     AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSL
                     KYLKKDGFAIFLAPVNLLTSPQSQLLKQWLKDYAQVVTLITLPDSIFGHPSNAKSIIV
                     LQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN"
     gene            4777..5973
                     /locus_tag="ACGEUI_001027"
     CDS             4777..5973
                     /locus_tag="ACGEUI_001027"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFF8165871.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEEAVLAQGIIERIGLKDSISTV
                     KYDGKKEEQILDIHDHTEAVKILLNDLIHFGIIAAYDEITGVGHRVVAGGELFKESVV
                     VNDKVLEQIEELSVLAPLHNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYH
                     GKSVDTSMGFTPLAGPMMGTRSGDIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSG
                     VSGISSDMRDIEAGLQEDNPDAVLAYNIFIDRIKKCIGQYFAVLNGADALVFTAGMGE
                     NAPLMRQDVIGGLTWFGMDIDPEKNVFGYRGDISTPESKVKVLVISTDEELCIARDVE
                     RLKNTK"
     gene            6160..6468
                     /locus_tag="ACGEUI_001028"
     CDS             6160..6468
                     /locus_tag="ACGEUI_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165872.1"
                     /translation="MYVFIEPNDLKDYLITVYDERNRAIRKQTQVYCFYLMIVLFVAL
                     FFFFPCLTIRDLVSYKLPLIFAYLYLIIRSLVAKKMEALGKITTNSQKPPFDTSQVLC
                     "
     gene            complement(6551..7321)
                     /gene="proC"
                     /locus_tag="ACGEUI_001029"
     CDS             complement(6551..7321)
                     /gene="proC"
                     /locus_tag="ACGEUI_001029"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFF8165873.1"
                     /translation="MKIGIIGVGKMASAIIKGLKQTPHELIISGSSLERSKEIAEQLA
                     LPYAMSHQDLIDQVDLVILGIKPQLFETVLKPLHFKQPIISMAAGISLQRLATFVGQD
                     LPLLRIMPNMNAQILQSSTALTGNALVSQELQARVRDLTDSFGSTFDISEKDFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKNGIPKAKALEIVTQTVLASASNLKTSSQSPHDFIDA
                     ICSPGGTTIAGLMELERLGLTATVSSAIDKTIDKAKSL"
     gene            complement(7369..8436)
                     /gene="pepA"
                     /locus_tag="ACGEUI_001030"
     CDS             complement(7369..8436)
                     /gene="pepA"
                     /locus_tag="ACGEUI_001030"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFF8165874.1"
                     /translation="MTDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGL
                     GGIFGIRDSKAEKAPRILVAAHMDEVGFMVSDIKVDGTLRVVGIGGWNPLVVSSQRFT
                     LYTRTGQVIPLISGSVPPHFLRGANGSASLPHIEDIVFDGGFTDKAEAERFGITPGDI
                     IIPQSETILTANQKNIISKAWDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGL
                     RGAHVSTTKFDPELFFAVDCSPAGDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLT
                     TAEEAGVNFQYYCGKGGTDAGAAHLQNGGVPSTTIGVCARYIHSHQTLYAMDDFVEAQ
                     AFLQAIIKKLDRSTVDLIKCY"
     gene            8901..9185
                     /locus_tag="ACGEUI_001031"
     CDS             8901..9185
                     /locus_tag="ACGEUI_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HFF8165875.1"
                     /translation="MSKKKIGMISGIFGFSLAIGLGIVIKDYCQDRQRRQMTRDLRTF
                     FSPLGQIEVLYINPCQVKQDYISGGVVMSNGKQYQFTYHSRQISFEEDKG"
     gene            9182..9499
                     /locus_tag="ACGEUI_001032"
     CDS             9182..9499
                     /locus_tag="ACGEUI_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFF8165876.1"
                     /translation="MIRPTSYESLATLIEKEDKLVLFFTADWCPDCQFIYPIMPEIEA
                     ELTDMTFVCVNRDQFIEVAQKWNIFGIPSFVVIEKGQEVGRLVNKMRKTKTEIMHFLA
                     AYQ"
     gene            9517..10143
                     /gene="ytpR"
                     /locus_tag="ACGEUI_001033"
     CDS             9517..10143
                     /gene="ytpR"
                     /locus_tag="ACGEUI_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFF8165877.1"
                     /translation="MIFAYNKEQVGDVLMVILQDTKDIKRQVERKGKVARVFAEESGK
                     TLAWNIFEASSLITIEGNGQIFLTDENLARLNAELAKEGFSERLEPIVGPVFVVGQIV
                     EMVAHPDSDHLNICQVAIGEDQTVQIVAGAPNAALGLKTIVALPGAIMPNGSLIFPGK
                     LRGEESYGMMCSPRELALPNAPQKRGIIEFDESAVVGEAFDPAKHWKG"
     gene            10295..10690
                     /locus_tag="ACGEUI_001034"
     CDS             10295..10690
                     /locus_tag="ACGEUI_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8165878.1"
                     /translation="MYNKVIAIGRLVAKPELVKTATDKHVARLSLAVNRRFKNASGER
                     EADFISVVVWGKLAETLVSYASKGSLMSIDGELRTRKYDKDGQVHYVTEVLCQSFQLL
                     ESRAQRAMRENNVTNDLVDLVLEEDTLPF"
     gene            complement(10950..11591)
                     /locus_tag="ACGEUI_001035"
     CDS             complement(10950..11591)
                     /locus_tag="ACGEUI_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8165879.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGEHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIYFLNKRFKSIKEAYQADNNILDRSIFEDELFLKLNYKNGNVTKTELD
                     IYQELLANMLEELEGMPKKRPDLLIYIDVSFDKMLERIERRGRSFEQVDGNPSLEQYY
                     HQVHGEYPTWYEDYEVSPKMKIDGNSLDFVQNPQDLATVLKMIDTKLKELHLL"
     gene            complement(11611..12588)
                     /gene="dusB"
                     /locus_tag="ACGEUI_001036"
     CDS             complement(11611..12588)
                     /gene="dusB"
                     /locus_tag="ACGEUI_001036"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFF8165880.1"
                     /translation="MIKLNSSFRIGDVEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQTNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAK
                     FMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETGQELPDLPFAKKLDIAKDHLKRLIN
                     LKGETIAVREFRGLAPHYLRGTAGAAKVRGAVSRAETLAEVEAIFETVR"
     gene            complement(12575..13447)
                     /gene="hslO"
                     /locus_tag="ACGEUI_001037"
     CDS             complement(12575..13447)
                     /gene="hslO"
                     /locus_tag="ACGEUI_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFF8165881.1"
                     /translation="MDKIIKSIAQSGAFRAYVLDSTETVALAQEKHNTLSSSTVALGR
                     TLIANQILAANQKGDSKITVKVIGDSSFGHIISVADTKGHVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVTIIDYGTGNPYTSTTPLITGEIGEDFAYYLTESEQTPSAIGLNV
                     LLDENDKVKVAGGFMVQVLPGASEEEIARYEKRLQEMPAISHLLASKNHVDALLEAIY
                     GDEPYKRLSEEPLSFQCDCSRERFEAALMTLPKADLQAMIDEDKGAEIVCQFCGTKYQ
                     FNESDLEAIISDKA"
     gene            complement(13594..15087)
                     /gene="rofA"
                     /locus_tag="ACGEUI_001038"
     CDS             complement(13594..15087)
                     /gene="rofA"
                     /locus_tag="ACGEUI_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047149466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RofA"
                     /protein_id="HFF8165882.1"
                     /translation="MIEKYLESSIESKCQLIVLFFKTSYLPITEVAEKTGLTFLQLNH
                     YCEELNAFFPGSLSMTIQKRMISCQFTHPFKETYLYQLYASSNVLQLLAFLIKNGSHS
                     RPLTDFARSHFLSNSSAYRMREALIPLLRNFELKLSKNKIVGEEYRIRYLIALLYSKF
                     GIKVYDLTQQDKNTIHSFLSHSSTHLKTSPWLSESFSFYDILLALSWKRHQFSVTIPQ
                     TRIFQQLKKLFVYDSLKKSSHDIIETYCQLNFSAGDLDYLYLIYITANNSFASLQWTP
                     EHIRQYCQLFEENDTFRLLLNPIITLLPNLKEQKASLVKALIVFSKSFLFNLQHFIPE
                     TNLFVSPYYKGNQKLYTSLKLIVEEWMAKLPGKRDLNHKHFHLFCHYVEQSLRNIQPP
                     LVVVFVASNFINAHLLTDSFPRYFSDKSIDFHSYYLLQDNVYQIPDLKPDLVITHSQL
                     IPFVHHELTKGIAVAEISFDESILSIQELMYQVKEEKFQANLTKQLT"
     gene            15350..17623
                     /gene="cpa"
                     /locus_tag="ACGEUI_001039"
     CDS             15350..17623
                     /gene="cpa"
                     /locus_tag="ACGEUI_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCT-2 pilus tip adhesin"
                     /protein_id="HFF8165883.1"
                     /translation="MKKTRFPNKLNTLNTQRVLSKNSKRFTVTLVGVFLMIFALVTSM
                     VGAKTVFGLVESSTPNAINPDSSSEYRWYGYESYVRGHPYYKQFRVAHDLRVNLEGSR
                     SYQVYCFNLKKAFPLGSDSSVKKWYKKHDGISTKFEDYAISPRITGDELNQKLRAVMY
                     NGHPQNANGIMEGLEPLNAIRVTQEAVWYYSDNAPISNPDESFKRESESNLVSTSQLS
                     LMRQALKQLIDPNLATKMPKQVPDDFQLSIFESEDKGDKYNKGYQNLLSGGLVPTKPP
                     TPGDPPMPPNQPQTTSVLIRKYAIGDYSKLLEGATLQLTGDNVNSFQARVFSSNDIGE
                     RIELSDGTYTLTELNSPAGYSIAEPITFKVEAGKVYTIIDGKQIENPNKEIVEPYSVE
                     AYNDFEEFSVLTTQNYAKFYYAKNKNGSSQVVYCFNADLKSPPDSEDGGKTMTPDFTT
                     GEVKYTHIAGRDLFKYTVKPRDTDPDTFLKHIKKVIEKGYREKGQAIEYSGLTETQLR
                     AATQLAIYYFTDSAELDKDKLKDYHGFGDMNDSTLAVAKILVEYAQDSNPPQLTDLDF
                     FIPNNNKYQSLIGTQWHPEDLVDIIRMEDKKEVIPVTHNLTLRKTVTGLAGDRTKDFH
                     FEIELKNNKQELLSQTVKTDKTNLEFKDGKATINLKHGESLTLQGLPEGYSYLVKETD
                     SEGYKVKVNSQEVANATVSKTGITSDETLAFENNKEPVVPTGVDQKINGYLALIVIAG
                     ISLGIWGIHTIRIRKHD"
     gene            17616..18173
                     /gene="sipA"
                     /locus_tag="ACGEUI_001040"
     CDS             17616..18173
                     /gene="sipA"
                     /locus_tag="ACGEUI_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase-like pilus assembly factor
                     SipA"
                     /protein_id="HFF8165884.1"
                     /translation="MTRADFRTSNSSGELNKLSFSDKEAIKPLLIGLVKKIALILIGG
                     YLLFHFVFGFMIIKRNDMAPSVKAGDAILFYRLSQTYKVEEAVVYEDSKTSITKVGRI
                     IAQAGDEVDLTEQGELKINGHIQNEGLTFIKSREANYPYRIADNSYLILNDYYSQESE
                     NYLQDAIAKDAIKGTINTLIRLRNH"
     gene            18206..19228
                     /gene="fctA"
                     /locus_tag="ACGEUI_001041"
                     /gene_synonym="tee1"
     CDS             18206..19228
                     /gene="fctA"
                     /locus_tag="ACGEUI_001041"
                     /gene_synonym="tee1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCT-2 pilus major pilin FctA/T1"
                     /protein_id="HFF8165885.1"
                     /translation="MKLRHLLLTGAALTSFAATTVHGETVVNGAKLTVTKNLDLVNSN
                     ALIPNTDFTFKIEPDTTVNEDGNKFKGVALNTPMTKVTYTNSDKGGSNTKTAEFDFSE
                     VTFEKPGVYYYKVTEEKIDKVPGVSYDTTSYTVQVHVLWNEEQQKPVATYIVGYKEGS
                     KVPIQFKNSLDSTTLTVKKKVSGTGGDRSKDFNFGLTLKANQYYKASEKVMIEKTTKG
                     GQAPVQTEASIDQLYHFTLKDGESIKVTNLPVGVDYVVTEDDYKSEKYTTNVEVSPQD
                     GAVKNIAGNSTEQETSTDKDMTITFTNKKDFEVPTGVAMTVAPYIALGIVAVGGALYF
                     VKKKNA"
     gene            19238..19951
                     /gene="srtC1"
                     /locus_tag="ACGEUI_001042"
     CDS             19238..19951
                     /gene="srtC1"
                     /locus_tag="ACGEUI_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCT-2 pilus polymerization class B sortase
                     SrtC1"
                     /protein_id="HFF8165886.1"
                     /translation="MIVRLIKLLDKLINVIVLCFFFLCLLIAALGIYDALTVYQGANA
                     TNYQQYKKKGVQFDDLLAINSDVMAWLTVKGTHIDYPIVQGENNLEYINKSVEGEYSL
                     SGSVFLDYRNKVTFEDKYSLIYAHHMAGNVMFGELPNFRKKSFFNKHKEFSIETKTKQ
                     KLKINIFACIQTDAFDSLLFNPIDVDISSKNEFLNHIKQKSVQYREILTTNESRFVAL
                     STCEDMTTDGRIIVIGQIE"
     gene            19973..20620
                     /gene="fctB"
                     /locus_tag="ACGEUI_001043"
     CDS             19973..20620
                     /gene="fctB"
                     /locus_tag="ACGEUI_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCT-2 pilus minor pilin FctB"
                     /protein_id="HFF8165887.1"
                     /translation="MKKSILRILAIGYLLMSFCLLDSVEAENLTASINIEVINQVDVA
                     TNKQSSDIDETFMFVIEALDKESPLPNSVTTSVKGNGKTSFEQLTFSEVGQYHYKIHQ
                     LLGKNSQYHYDETVYEVVIYVLYNEQSGALETNLVSNKLGETEKSELIFKQEYSEKTP
                     EPHQPDTTEKEKPQKKRNGILPSTGEMVSYVSALGIVLVATITLYSIYKKLKTSK"
     gene            complement(20807..22159)
                     /locus_tag="ACGEUI_001044"
     CDS             complement(20807..22159)
                     /locus_tag="ACGEUI_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element short variant transposase"
                     /protein_id="HFF8165888.1"
                     /translation="MTRTNYQKKRMTCPVETEDITYRRKKIKGRRQAILAQFEPELVH
                     HELIGDSCTCPDCHGTLTEIGSVVQRQELVFIPAQLKRINHVQHAYKCQTCSDNSLSD
                     KIIKAPVPKAPLAHSLGSASIIAHTVHQKFTLKVPNYRQEEDWNKLGLSISRKEIANW
                     HIKSSQYYFEPLYDLLRDILLSQEVIHADETSYRVLESDTQLTYYWTFLSGKHEKKGI
                     TLYHHDKRRSGLVTQEVLGDYSGYVHCDMHGAYRQLEHAKLVGCWAHVRRKFFEATPK
                     QADKTSLGRKGLVYCDKLFALEAEWCELPPQERLVKRKEILTPLMTTFFDWCREQVVL
                     SGSKLGLAIAYSLKHERTFRTVLEDGHIVLSNNMAERAIKSLVMGRKNWLFSQSFEGA
                     KAAAIIMSLLETAKRHGLNSEKYISYLLDRLPNEETLAKREVLEAYLPWAKKVQTNCQ
                     "
     gene            complement(22092..22319)
                     /locus_tag="ACGEUI_001045"
     CDS             complement(22092..22319)
                     /locus_tag="ACGEUI_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8165889.1"
                     /translation="MSSQDKVIKTQAKTIETMANELALLREQVAYLTQKLYGKSSEKA
                     MPMTGQLSLFDQDQLPEEADDLPSRNRRHYL"
     gene            complement(22370..22720)
                     /gene="tnpB"
                     /locus_tag="ACGEUI_001046"
     CDS             complement(22370..22720)
                     /gene="tnpB"
                     /locus_tag="ACGEUI_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFF8165890.1"
                     /translation="MTIRLNDLGQVYLVCGKTDMRQGIDSLAYLVKSQHELDLFSGAV
                     YLFCGGRRDRFKALYWDGQGFWLLYKRFENGKLAWPRNRDEVKCLTAVQVDWLMKGFF
                     ISPNIKISKSHDFY"
     gene            complement(22701..22885)
                     /locus_tag="ACGEUI_001047"
                     /pseudo
     CDS             complement(22701..22885)
                     /locus_tag="ACGEUI_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935182.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22950..23779
                     /locus_tag="ACGEUI_001048"
                     /pseudo
     CDS             22950..23779
                     /locus_tag="ACGEUI_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090262.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            complement(23973..24638)
                     /locus_tag="ACGEUI_001049"
     CDS             complement(23973..24638)
                     /locus_tag="ACGEUI_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165891.1"
                     /translation="MKRCNKYLFTSLLAASMLFSSYKSVHAHDNIDEKGKVHLYWQGN
                     YYVDNYVDYTKKLVDSDKNIEWTVTFNSAKEQWVYPDFSVFLPKGVKAPTEITYEHHY
                     WDGTVRSETRNNTQWHYDWKSQQTNFNQEFDKFPGYTGWSPSLDKFYKLKNDGKFSHV
                     LVDTYGRQSHTYFSHKMVWKFKTELEDNYKNKWDKLPFIAGIKQNNPLAASFPSYKGE
                     FGE"
BASE COUNT         7887 a         4228 c         4974 g         7565 t
ORIGIN      
        1 ttctgatatt ttcttttttt gtttacgaat aaatgagtaa ggaggaatta tggtacaagc
       61 attagcaaaa gctattctag caaaagctga acaggttcat gcacaagata tttatatttt
      121 gccaagagca gatcaatatg atcttttttt acgaatagga gatgaaagga gattagttga
      181 tgtttatcag agcgatcgga tggctcctct tattagtcac tttaaattcg ttgcaggaat
      241 gatagttggt gaaaagagac gttgtcaggt gggttcatgt gattataagc ttagtaaaga
      301 taagcagtta tctttgcgct tatctagcgt gggtgattat cgcgggcaag aaagcttagt
      361 gattcgtctg cttcatcatc aaaataaaag tgtacattat tggtttgatg gattgacaaa
      421 agtagccaat caggttggcg gtagagggtt gtatttattt gcaggaccag ttgggtctgg
      481 gaagacaacc ttgatgtacc agctgatttc gaattatcat caagaagcac aggttattag
      541 tatagaagat cctgtagaaa ttaaaaatca ccaaatttta caattacaag tgaatgatga
      601 tattggtatg acttatgaca atttgatcaa actgtcttta cgccatcgac cagatatttt
      661 agttattggt gagattcgag atagtcaaac agcaagagcc gttattaggg ctagtctaac
      721 aggtgccatg gttttttcaa cggttcacgc taaaagtatc tcgggtgttt atgcaagatt
      781 gttagaactt ggtgtaacga aagcagaact gtctaattgc ttagcattaa ttgcttacca
      841 aaggttactt aatggaggag cattgattga ctctactcaa aacgaatttg aatattattc
      901 ctcatcgaac tggaatcaac aaattgatca gcttcttgag gcaggacatc tcaatcccaa
      961 gcaagctaag cttgaaaaaa ttatctagta aacatcagca taaattcatt caattactag
     1021 ctaatctttt atctacagga tttagcttcg cagaggttat tgctttttta aaaagaagcc
     1081 agttgcttca gctagattat gttcttaaaa tggaagagtc tttattaaaa ggacaaggct
     1141 tagcagatat gttgtcgggt ttaggttttt cagatgctat tcttactcaa attagtttag
     1201 ctgatagaca cggtaatatt gaaacaacat tagtagcgat tcaacattac ttgaaccaaa
     1261 tggcaaggat cagacgaaaa actgttgaag ttatcaccta tcctcttatt cttttacttt
     1321 ttctttttgt gatgatgcta gggttgagac gctatttagt ccctcaatta gaaactcaaa
     1381 atcagataac ttactttctt aaccattttc ctgccttctt tataggtttt tgctcaggtc
     1441 ttattttgtt gttcgggatg gtatggctac gatggcgatc tcaaagccgc ttgaaactct
     1501 atagtcggct aagccgttat ccttttttag gtaaactgtt aaaacaatac ttaactagtt
     1561 attatgctag agaatggggt acgttaatag gtcaaggtct tgatttgatg actattttag
     1621 acattatggc cattgagaaa tcctcactaa tgaaagaatt agctgaggac atcagaatga
     1681 gtctgcttga agggcaagcc tttcacatta aagtagctac ctaccctttt tttaaaaaag
     1741 aattaagttt aatgattgaa tacggtgaaa tcaaatcgaa actgggggct gagttggaaa
     1801 tctatgccca ggaaagctgg gaacaatttt ttagtcagct ttatcaagtg acacaactta
     1861 ttcaaccagc tattttttta gtagttgctg tgacaatcgt catgatttat gcggcaatcc
     1921 tattaccaat ttaccaaaat atgggaggta ttttttaaga tgattaatca atggaacaac
     1981 ttacgacaca agaagctaaa aggatttact cttctagaaa tgttattggt gattcttgtc
     2041 atcagtgttt tgatgctatt atttgtgcct aatttaagca agcaaaaaga cagggttaca
     2101 gaaacaggta atgccgctgt tgttaaatta gtggagaatc aagcagaact atatgaatta
     2161 tctcaaggct caaaaccaag tttgagccag ttaaaggcag atggtagtat cactgagaaa
     2221 caagaaaaag cttatcaaga ctattatgac aaacataaaa atgaaaaagc ccgtcttagc
     2281 aattaaagct tttactttgc ttgaaacttt actaagtttg tcagtgatga gtttcattat
     2341 attgggattg tctgtaccag tgacaaaaag ctatcaaaaa gtagaagagc atctgttttt
     2401 tagccatttt gaacatttgt atcgacatca gcaaaagtta gccatattac agcaaaaaca
     2461 gcgcgtcttg gatatttctt caacaaaaat tgttacagaa ggtaatagcc taacagttcc
     2521 taaatcaata acagtgaatc atccttatcg gttagttatt gatcagatgg gaggcaacca
     2581 ttccttagct aaaattattt ttgacatgac tgatcgacgc tttaaatacc aattttatct
     2641 agggagtggg aattatcaaa aaacaagtca aagcttacat agcccttgaa agtatgatcg
     2701 caacaggcat cctctttagt attgtcattc tcgtcttaag cagtttacag cagagtcagg
     2761 ctgctctaac gtactatcga aagcagcaag aaaagcttaa tctagccttg atggcagtac
     2821 aaactagaac taaagagatg acattgaatg gttgtcatat tactatttta cgtacggata
     2881 gatacattag tattcacgat gacgaaggag aagtgatgaa gattgagtaa acaattaagt
     2941 aacataaaag cttttaccct tctagaggcg ttaatagcct tactcgtgat atcagggtct
     3001 ttattggttt atcaaggttt gacccgaacc ctccttaaac atagccatta tctagcccgt
     3061 catgatcaag ataattggct cttattttct catcaattgc gagaggagtt aagtggagca
     3121 aggttttaca aagtagctga taataaacta tacgttgaaa aaggaaagaa agtactagct
     3181 tttggccaat ttaaaagtca tgattttcga aaatcagcta gtaatggaaa agggtatcaa
     3241 cccatgttat ttggaatatc acgtagtcat attcacatag agcagtcaca gatttgcatt
     3301 actttaaagt ggaaaagtgg gttagaaagg actttttatt atgcctttca agactaatct
     3361 caaagcaggc atccttttgt atgctatgtt tatggctagt atttttttgc tggttttaca
     3421 ggtttacctt tctcaagtga cagctctaca caaggaatac caagctcaaa ctgactatgt
     3481 taaagcccga ctgatagcag aaatagttta tcaagatcat agatataaag cttctaatcc
     3541 tgtctttttt aaaggtggtc aagtcatttg tcgagagcga aaagagagat ggatgttaat
     3601 tgttaagtta gatcagcaac gacagtatca gtttgaatac cttaaaccca aacaatatag
     3661 tcaataaata gtattgttgt cataaatcct taaaatgatc caatatatag taattaaagg
     3721 tgacacttct tttcatttca agtgtaattt ggtatgataa ggttatgact tttgaaaaaa
     3781 ttgaagaagc ttatcagctg ctcttagaga actgtcaact aattgaaaat gatctaaaaa
     3841 cacatatata cgatgccatt gttgaacaaa actcctttta tttaggggct gagggagcta
     3901 gtcctcaagt tgctcaaaat agtgataaac tgaaagcctt gtgcctgaca aaagaagaat
     3961 ggcgtaaggc ctaccagttt ctttttatta aggcagctca gactgagcaa ctccaagcca
     4021 accatcagtt cacaccagat gctattggct tcattctgct gtatcttttg gaacaattga
     4081 gtgataaaga tagcttagag gtacttgaga ttggaagtgg aacagggaac ctagcccaaa
     4141 cccttctcaa caacacgagc aagagccttg attatgtagg gattgaactt gatgatctct
     4201 tgattgatct gtcagccagt attgctgaga taatggattc ttcagctcat tttattcaag
     4261 aagatgcggt aaggcctcaa ttactaaaag aaagtgacat tgtcatcagt gacttaccag
     4321 ttggttatta tcctaacgat gatattgcca aacggtacaa ggtggctagt tcagataagc
     4381 atacctatgc ccatcattta ttaatggaac agtctttaaa atacttgaaa aaagacggtt
     4441 ttgcgatttt tctggcacca gtcaatttat tgacgagccc tcagagccag ttgttgaaac
     4501 agtggttaaa agattatgct caggtggtga ccttgattac gctaccagat tctatttttg
     4561 gtcatccctc aaatgccaag tccattattg tcttacaaaa acaaacagac cacccaatgg
     4621 aaacctttgt ctatccaatt cgggatttga agcttgcaga gaatattcat gattttatgg
     4681 aaaatttcaa aaaatggaaa ctgagtaatg tcaattaatt gattttttgt tataataaaa
     4741 aagcatcaca gaaaacgatt tcaagagagg ttaaaaatgt caaagacaat tgcaattaat
     4801 gcaggtagtt ctagtttaaa atggcaactt tatcagatgc cagaagaagc agtgttagca
     4861 caaggaatta ttgagcgtat cggtctcaag gattctattt caacagttaa gtacgatggc
     4921 aaaaaagaag agcaaattct tgatattcac gatcatacag aagctgttaa aatcttattg
     4981 aacgatttga ttcactttgg aatcattgca gcttatgatg aaattacagg agttggacac
     5041 cgtgttgttg ctggtggtga acttttcaaa gaatccgttg tggttaatga caaagttctt
     5101 gaacagattg aagaattatc cgttttagct ccacttcaca atcctggagc ggcggcaggg
     5161 attcgtgcct tccgtgatat tttgccagat attaccagtg tttgtgtgtt tgacacgtca
     5221 tttcacacta gcatggcaaa gcatacttac ctttacccaa ttcctcaaaa atattatacg
     5281 gactacaagg ttcgtaaata tggggcgcat gggacaagtc ataaatatgt agcgcaagaa
     5341 gctgctaaaa tgcttggtcg tccattagaa gaattaaaac taattactgc tcatattgga
     5401 aatggtgtct caattacagc taattaccac ggtaaatcag ttgatacctc gatgggcttt
     5461 acaccacttg ctggtcctat gatgggaaca cgttctggtg acattgaccc agctatcatt
     5521 ccttatttaa ttgagcaaga cccagagtta aaagacgctg ctgacgttgt taatatgtta
     5581 aacaaaaaat caggtcttag tggggtgtca ggcatttcta gtgatatgcg tgatatcgag
     5641 gctggtttac aagaagataa tccagatgct gttttagctt acaatatctt tattgatcgc
     5701 attaaaaaat gcattggtca atattttgct gttttgaatg gagcagatgc tttggtcttt
     5761 acagctggta tgggtgaaaa tgcaccattg atgcgtcaag atgttattgg tggcttaaca
     5821 tggttcggaa tggacattga tcctgagaaa aatgtttttg gataccgtgg tgacatttca
     5881 actcctgagt caaaagtgaa ggtgcttgtt atctcaacag acgaagaatt gtgcatcgcg
     5941 cgtgacgttg aacgtttaaa aaatacaaaa taaaagagac taagtctcaa tagattaacc
     6001 aagtaaggta acactttact tggtttttta tgaggtttat ctgttggtta tggtgcctag
     6061 gtattagttc tgatcagtct ccttgctttt tgtaatcttg tgctaggcga tttggatgtc
     6121 agccttattt agaagttttt aattgcttca acgcttttct tgtatgtgtt tatagagccc
     6181 aatgatctaa aagactattt gataacggtt tatgatgagc gtaatcgtgc gattcgaaaa
     6241 cagacacaag tttactgctt ttacctgatg attgttctat ttgttgcctt attctttttc
     6301 ttcccttgct taaccattcg agatcttgtt tcttataagc tacctttgat ttttgcttac
     6361 ctttacttga ttatcagaag tctggtggct aagaaaatgg aggcactagg gaaaataaca
     6421 actaatagtc aaaaacctcc gtttgatacg agtcaggttc tttgttagat gacttgtcta
     6481 acggttgagt ttcctgtctg gtcaacggag gtgtttgtat tgttgtctga tttgtattgg
     6541 ggcacttatt ttacaagctt ttagctttat cgatggtttt gtcaatggca gagctgacag
     6601 tagctgtgag gccaaggcgt tctaactcca tcagaccagc aatagttgtg ccaccggggc
     6661 tacaaatagc gtcaatgaaa tcgtgcggac tttgagaact ggtcttgaga ttgctggcgc
     6721 tagccaatac tgtttgagta acaatctcca gcgcctttgc tttaggtatg ccattcttga
     6781 cgccagcctt agccaaagcc tcaataaaga gataaatata ggcaggactt gaccctgcta
     6841 aagcggtaaa ggtgtcaaaa tccttttcac taatatcaaa tgtgctacca aagctatctg
     6901 ttaagtctcg aacacgtgct tgtaattcct gggacaccaa agcatttccc gttaaagcgg
     6961 tactgctttg gagaatttgt gcattcatgt ttggcatgat acgtagcagc ggaaggtctt
     7021 gtcctacgaa tgttgctagt cgttgaaggg aaatgcctgc tgccatagat ataataggct
     7081 gtttgaagtg aagcggtttg agtaccgttt caaatagttg aggcttgatg cctaaaataa
     7141 caagatcaac ctggtcaata aggtcttggt gggacatagc ataaggcagt gctaactgct
     7201 ccgcaatttc cttggaccgt tctaaagatg atcctgaaat gataagttca tggggtgttt
     7261 gtttaaggcc tttgatgata gcgctagcca ttttgccaac accaataatg ccaattttca
     7321 ttctgttgct ccttgtggga tattatggtt aatcaggcta atgctagttt agtaacattt
     7381 aatcaagtca acggttgagc gatccagttt tttgataatg gcttgtaaga aggcttgggc
     7441 ttctacgaaa tcatccatag cgtagagggt ttgatgagag tgaatgtagc gtgcacagac
     7501 tccgatggtt gttgatggga caccaccatt ttgaaggtgt gcagcacctg catctgtgcc
     7561 tcccttgcca caatagtatt ggaaattgac accagcttcc tcagcagtag tcagtaagaa
     7621 gtcgcgcata tctttgagca tgacatggcc tgggtcgtag aaacgcaaca aggtaccatc
     7681 tccgattgtt ccaggattgc cataaatatc accagcaggc gaacaatcta ctgcgaaaaa
     7741 gagttcaggg tcgaacttgg tggttgagac gtgggctccg cgcagaccaa cttcttcttg
     7801 aacgttagca cctgcaatta gggtgttgtt aaggtcttgt ccttttaacg cttcaagcat
     7861 ttctgttatc atgagaacgc catagcgatt gtcccaagct tttgaaataa tatttttttg
     7921 attggctgtt aggattgttt cagattgagg gataataata tcacccggtg taataccaaa
     7981 tctttcagct tctgccttat ccgtaaagcc accatcaaac acaatatctt cgatatgtgg
     8041 tagactagca gagccatttg ccccacgtaa aaaatgggga ggtaccgatc ctgaaataag
     8101 gggaataact tggccagtgc gtgtgtaaag ggtaaaccgt tgtgaactga caacaagtgg
     8161 gttccaacca ccgataccaa ccacgcgtag cgttccgtca actttgatat cactgaccat
     8221 aaaaccgact tcgtccatgt gcgcagctac taaaatacgg ggggcttttt cagctttact
     8281 atctctgata ccaaaaatgc caccaagccc gtctgtttca acacggtcaa ccagcggggt
     8341 tattttggtg cgtaggtagt cacgaacgct atgttcatag cccgcaatgc catccagttc
     8401 ggtaacttct ttgatttttg agaataagtc tgtcattttt catctccatt taaaaggttt
     8461 cagaccagtt caacgcggtc atctccttat attctaccac tttttaagta agaatgggtt
     8521 tcacagaaaa gaaaaatccc agagactaga gatctctgga agaagtgttt gtcttttggc
     8581 gataacctgt ttttttaggc agcaaggaaa tcattaaaat gataaagcac aagctggtta
     8641 ataagaatag tagggcgctt aggatgcgaa aagactcttc actagcgaaa agtaaggata
     8701 atggagtcat aaggagaacc ttccatttta ggttaatcag tgatttttat agcgtggtta
     8761 aattaataat aaaccacacc taatcaacgt acctttagtt tagttgtttt tactattttt
     8821 gtcaatacat aacagtagaa atctttagtt ttcttatttc agatcgtcca atttgatctt
     8881 tttatgataa aatgagggat atgagtaaaa agaaaatagg tatgatttca ggtatctttg
     8941 gatttagttt agctattggc ctaggaatag ttatcaaaga ttattgccaa gacaggcagc
     9001 gtcgacaaat gacaagggat ttacgcacct ttttttcacc tttaggacaa atcgaagttt
     9061 tatatatcaa tccttgtcag gtcaaacaag attatatttc aggtggggtt gttatgtcaa
     9121 acggcaaaca gtaccaattc acctatcaca gtcgacaaat tagttttgag gaggacaagg
     9181 gatgatcaga ccgacttctt atgagtcatt agcgactctt attgaaaaag aagataagct
     9241 ggtgttattt tttacggcag actggtgtcc agactgtcaa tttatttatc ctattatgcc
     9301 agaaattgaa gcagaactta cagatatgac ttttgtttgt gttaatcgtg atcagtttat
     9361 agaggtagca caaaagtgga atatttttgg tattcctagt tttgtcgtga ttgaaaaagg
     9421 tcaggaagta gggcgtctgg ttaacaaaat gagaaagaca aaaactgaga ttatgcattt
     9481 tttagctgct tatcaataag aaaagaggaa gaataaatga tttttgcata caataaagaa
     9541 caagttggcg atgtcttgat ggttatctta caagacacca aagatatcaa acgtcaagta
     9601 gaacgaaaag gcaaagtagc ccgtgttttt gcagaagaaa gcggcaaaac ccttgcttgg
     9661 aatatctttg aagcgtcaag cttgattact attgaaggca atggacagat ttttttgaca
     9721 gacgagaacc ttgcaagatt aaatgcagag cttgctaagg aaggattttc agaaaggctt
     9781 gaaccgattg tgggacctgt ttttgtggtt ggtcaaattg ttgagatggt ggctcatcca
     9841 gatagcgacc atcttaatat ctgccaagtg gctattggtg aagatcaaac ggttcaaatc
     9901 gtagcgggcg caccaaatgc tgcacttggt ttaaaaacga ttgttgcctt accaggtgct
     9961 ataatgccaa atggtagtct gattttccca ggaaaattgc gtggtgaaga aagttatggt
    10021 atgatgtgct ctcctcgtga gttagccttg ccaaatgcac cgcaaaaacg tggcattatt
    10081 gaatttgatg agtcagctgt ggtaggagaa gcttttgacc cagccaaaca ttggaaaggt
    10141 tagaaaaacc agctgaaata agactgtatt taactaaatg accagaaaaa aaggattagg
    10201 agcccttaat ccttttttct tatgttttca aaaacgttta aaatgaaact tattttacca
    10261 caatttttac gaataataag ataaggaggt cagtatgtat aataaagtga tagcaatcgg
    10321 tcgtttggta gctaaaccag aattggtaaa aacagctacg gataagcatg tagcacgtct
    10381 ctctttagct gttaatcgaa gatttaaaaa tgcttctgga gagcgagaag ctgattttat
    10441 ttcagttgtt gtttggggaa agttagcaga aactctggtt tcttatgcta gcaaaggtag
    10501 tttgatgtct attgatggcg aacttaggac ccgcaagtat gataaagatg ggcaagtgca
    10561 ttatgtgaca gaagttctct gccaatcatt tcaactgctt gaaagtcgtg ctcagcgcgc
    10621 tatgagagaa aataatgtta ctaatgatct agttgattta gtcttagaag aagatactct
    10681 tcccttttga tagaggataa tgcttctcat cagggttcag ctgattactg aaatatatga
    10741 tgaggtgata tccgaacaac aaaagtgtga ctagatactt ctgataaaaa taaaaaaaca
    10801 ccttacgagt atgaacaaag tttcaagtgc tagatgttgg aactttgttc atcataggaa
    10861 tagtagggtg ttttttagtg tattacttct ttttgttgct agtgttaagg tttacttgct
    10921 tacaagcggt tatcaggcca ctagaactct tataataaat ggagctcttt gagtttggtg
    10981 tcaatcattt ttaagacagt cgccaaatct tgtgggtttt gaacgaagtc aagggaattg
    11041 ccatcaattt tcatttttgg agagacctcg tagtcttcgt accaggttgg gtattcaccg
    11101 tggacttgat ggtaatattg ctcaagacta ggattgccgt cgacctgctc aaaacttcgg
    11161 ccacgtcttt caatgcgctc aagcattttg tcaaaagaca catcaatgta gatgagaaga
    11221 tcagggcgtt ttttaggcat gccttctagt tcttctaaca tgttggcgag aagttcctga
    11281 tagatgtcaa gttctgtctt agtgacattg ccatttttgt agttaagttt taagaagagt
    11341 tcgtcttcaa aaatagagcg gtcaagaatg ttgttatcgg cttgataagc ttctttgatg
    11401 gacttaaatc gtttattcaa aaaataaatt tgaagtaaaa aggcgtattt tttagggtct
    11461 tggtagtaga ggtctagcac ggggttatta tcaacagctt cgtaaaaaac gtcagttcct
    11521 aaatgctctc caagtgcagc agcaagcgag ctcttgccag ccccaattgt tccagctagt
    11581 acaatcaaca tgcgtctcct tctaatatca ttagcgtact gtttcgaaga tggcttcaac
    11641 ctcagctaga gtctcagcgc gtgaaacagc accacgaact ttggcagcac ctgctgttcc
    11701 gcgaagatag tgtggtgcta agccccgaaa ttcccgaaca gcaatggttt cacctttgag
    11761 gttaatcagg cgtttaaggt ggtctttggc gatgtctaat tttttggcaa agggaagatc
    11821 tggcaattct tgaccagttt caaagaaatg attgatttgg gtaaaaagat aaggattgtt
    11881 catggctgcg cggccaatca tcacggcatc tacaccgatt tcttcaatca taaatttggc
    11941 gtcttgcacc gaacgcacgt ccccatttcc aataaaggga atcttagtga tagccttaga
    12001 aactcgggct aatgtctcat ggtcgcaggt tcctgtatac atttgttcac gggtacgacc
    12061 gtgcatggca agagcagata cacctgccga ctcagcggca agggcatttt caacagcaag
    12121 tgaactatcc gcccaacctg tacgcatttt gacagtcagt ggaatatcta aaacagacgt
    12181 gacttccttg acaatgtggt aaatcttatc agggtcacgg agccacttgg caccagcctc
    12241 gtttttgaca actttattaa cagggcagcc catattaata tcaacaatat cggccttggt
    12301 attggtttga atgaaatcag ctgcacgttt caacccttct gcgtcaccac caaataattg
    12361 aatggacata gggtgctcat tttcatcaat atggagcatg tgaagggtct tttcattgtt
    12421 gtaaagaagc cctttttcgg aaatcatttc cataaccacc aaaccagcac caaattcttt
    12481 ggcgatggta cgaaaggcag aattggtcac gccagccatg ggagcaagga cagtgcggtg
    12541 agggatttcc acatcaccaa tcctaaagga agaattaagc tttatcactg ataatggcct
    12601 ccaaatcact ttcattaaat tggtacttgg taccacaaaa ttggcaaaca atctcagcac
    12661 ccttatcttc gtcaatcatg gcctgtaaat ctgcttttgg caaagtcatt agggctgctt
    12721 caaaacgttc tctagaacag tcacactgaa aactcagagg ttcttcagaa agacgcttgt
    12781 aaggttcatc tccatagatg gcctccaaaa gggcatccac atgatttttg gaagctagta
    12841 ggtgagaaat ggcaggcatt tcttgcaggc gtttttcata gcgagcaatc tcttcttcag
    12901 aagcgcctgg tagcacctgc accataaagc caccagcaac cttaacctta tcattttcat
    12961 ctaaaagcac attaagtccg attgcagatg gtgtttgctc tgattcggtt aagtagtagg
    13021 caaaatcctc accaatttcc ccagtaataa gaggcgttgt tgaggtgtaa gggttacctg
    13081 tgccataatc aataatcgtc acaaaatgac cattccccat aaaaggccca acaaggactt
    13141 cacctgttgc ggtcttttta atatcgacac cagtattttg aatgtaacct ttcacatgtc
    13201 ctttggtatc tgccacagag ataatgtgtc caaaagaaga atcaccaatc actttgacgg
    13261 tgattttact atcgcctttt tgattagccg ccaaaatctg gttggcgatc aaggtacggc
    13321 caagcgcaac agtagacgat gacagcgtgt tgtgtttttc ttgagctaat gcaaccgtct
    13381 cagtgctatc aaggacataa gccctaaaag cccctgattg tgctattgat ttaattattt
    13441 tatccataac ttagattata gcataaagtg taaaacttgt attgacagga ttgcttgaaa
    13501 aatcaataca gatagacagt aaaaaatccc agagaatgac tcgggattaa gttaaggtgt
    13561 ggtctaaagt cgcgcaatgt ggtgacatgg cgcttatgtt aattgcttgg ttaaattagc
    13621 ttggaatttt tcctctttaa cttgatacat caattcttgg atagacagaa tcgattcatc
    13681 aaaagatatt tcagcaacag caattccttt tgtaagttca tggtgaacaa aaggaatcag
    13741 ttgactgtga gtgatgacca aatctggctt taaatcagga atttgataaa cattatcttg
    13801 caatagataa taggaatgaa aatcaatgct tttatccgag aaataccttg gaaaagaatc
    13861 cgttaggaga tgagcattga taaaattact ggctacgaaa acaacaacta aaggaggttg
    13921 gatatttctt agactttgct cgacatagtg gcaaaaaaga tgaaaatgct tatggttcaa
    13981 gtcacgctta ccaggaagtt tggccatcca ctcttcgaca attaacttta aggacgtata
    14041 gagtttttgg tttcctttat agtacggaga aacgaataag ttggtctcag gaataaaatg
    14101 ttgcagatta aacaagaatg attttgaaaa aacaataaga gcttttacta aactagcctt
    14161 ttgctctttt aggttaggta aaagagtgat gataggattt aaaagcaggc gaaaagtatc
    14221 attttcttca aaaagttgac aatattgtct gatatgctca ggtgtccatt gtaagctcgc
    14281 aaaagaatta ttagcggtga tataaattaa atagaggtag tccaaatctc ctgctgaaaa
    14341 gtttagttgg cagtaagttt cgataatatc atggctactt tttttcaaag aatcgtagac
    14401 aaaaagtttt tttaattgtt gaaaaattct ggtttgggga atagttaccg aaaattgatg
    14461 ccgcttccac gataaagcta ataaaatgtc atagaaagaa aacgattccg ataaccaagg
    14521 agaggtttta aggtgggtgg aactatggga taaaaagcta tgaatagtgt ttttgtcttg
    14581 ctgcgtcaag tcataaactt taatgccaaa cttactatat agcagagcga tgaggtaacg
    14641 gatgcgatat tcctcaccga caatcttgtt cttagagagt tttaattcaa agtttcttaa
    14701 taaaggaatc aatgcttcgc gcatccgata agctgaggag tttgataaaa aatgacttct
    14761 tgcaaaatcc gtaaggggac gagagtggga accatttttt attaaaaagg ctagtaattg
    14821 taagacatta gatgatgcat agagttggta aagataagtt tctttaaaag gatgtgtaaa
    14881 ttggcaagat atcatccttt tttggatggt catagacaga ctaccaggga aaaaggcatt
    14941 cagttcctca caataatggt ttagttgtaa aaaggttaag ccagtttttt ctgctacctc
    15001 agttattggc aaataagatg tcttaaaaaa caagacaatt aactgacatt ttgattcgat
    15061 tgatgattcc aagtattttt ctatcaagga aagggacctc tcttcctaca cttagaaagc
    15121 caaataagtc tttgataagt gtattctcct atttcaattc tatctaagct aaaaatagaa
    15181 gtcaaatcta atggtttttg gtaaaaagtc gaaacctttt gaaatagctt taaagcaata
    15241 ttttctcaaa aaatcatttt caaaaacact aatttggtga aaaacttagt acgaatatat
    15301 ttctttgact tcaatagaat gatatgatgt cacattgaga ggagagaaaa tgaaaaaaac
    15361 aaggtttcca aataagctta atactcttaa tactcaaagg gtattaagta aaaactcaaa
    15421 acgatttact gtcactttag tgggagtctt tttaatgatc ttcgctttgg taacttccat
    15481 ggttggtgct aagactgttt ttggtttagt agaatcctcg acgccaaacg caataaatcc
    15541 agattcaagt tcggaataca gatggtatgg atatgaatct tatgtaagag ggcatccata
    15601 ttataaacag tttagagtag cacacgattt aagggttaac ttagaaggaa gtagaagtta
    15661 tcaagtttat tgctttaatt taaagaaagc atttcctctc ggatcagata gtagtgttaa
    15721 aaagtggtat aaaaaacatg atggaatctc tacaaaattt gaagattatg cgataagccc
    15781 tagaattacg ggagatgagc taaatcagaa gttacgagct gttatgtata atggacatcc
    15841 acaaaatgcc aatggtatta tggaaggctt ggaacccttg aatgctatca gagttacaca
    15901 agaggcggta tggtactatt ctgataatgc tcctatttct aatccagatg aaagttttaa
    15961 aagggagtca gaaagtaact tggttagtac ttctcaatta tctttgatgc gtcaagcttt
    16021 gaagcaactg attgatccga atttggcaac taaaatgcca aaacaagttc cggatgattt
    16081 tcagctaagt atttttgagt ctgaggacaa gggagataaa tataataaag gataccaaaa
    16141 tcttttgagt ggtggtttag ttcctactaa accaccaact ccaggagacc caccaatgcc
    16201 tccaaatcaa cctcaaacga cttcagtact tattagaaag tatgctatag gtgattactc
    16261 taaattgctt gaaggtgcaa cattacagtt gacaggggat aacgtgaata gttttcaagc
    16321 gagagtgttt agcagtaatg atattggaga aagaattgaa ctatcagatg gaacttatac
    16381 tttaactgaa ttgaattctc cagctggtta tagtatcgca gagccaatca cttttaaggt
    16441 tgaagctggc aaagtgtata ctattattga tggaaaacag attgaaaatc ccaataaaga
    16501 gatagtagag ccttactcag tagaagcata taatgatttt gaagaattta gcgttttaac
    16561 tacacaaaac tatgcaaaat tttattatgc aaaaaataaa aatggaagtt cacaggttgt
    16621 ctattgcttt aatgcagatc taaaatctcc accagactct gaagatggtg ggaaaacaat
    16681 gactccagac tttacaacag gagaagtaaa atacactcat attgcaggtc gtgacctctt
    16741 taaatatact gtgaaaccaa gagataccga tcctgacact ttcttaaaac atatcaaaaa
    16801 agtaattgag aagggttaca gggaaaaagg acaagctatt gagtatagtg gtctaactga
    16861 gacacaattg cgtgcggcta ctcagttagc aatatattat ttcactgata gtgctgaatt
    16921 agataaggat aaactaaaag actatcatgg ttttggagac atgaatgata gtactttagc
    16981 agttgctaaa atccttgtag aatacgctca agatagtaat cctccacagc taactgacct
    17041 tgatttcttt attccgaata acaataaata tcaatccctt attggaactc agtggcatcc
    17101 agaagattta gttgatatta ttcgtatgga agataaaaaa gaagttatac ctgtaactca
    17161 taatttaaca ttgagaaaaa cggtgactgg tttagctggt gacagaacta aagatttcca
    17221 ttttgaaatt gaattaaaaa ataataagca agaattgctt tctcaaactg ttaaaacaga
    17281 taaaacaaac ctcgaattta aagatggtaa agcaaccatt aatttaaaac atggggaaag
    17341 tttaacactt caaggtttac cagaaggtta ttcttacctt gtcaaagaaa cagattctga
    17401 aggctataag gttaaagtta atagccaaga agtagcaaat gctacagttt caaaaacagg
    17461 aataacaagt gatgagacac ttgcttttga aaataataaa gagcctgttg ttcctacagg
    17521 agttgatcaa aagatcaatg gctatctagc tttgatagtt atcgctggta tcagtttggg
    17581 gatctgggga attcacacga taaggataag aaaacatgac tagagctgat ttcagaactt
    17641 ctaattcatc tggagaactt aacaaactat cattttcaga taaagaagca ataaagccct
    17701 tactcatagg acttgttaaa aaaatagccc ttattcttat tggcggctat ctactttttc
    17761 actttgtttt tggatttatg attatcaaaa gaaacgatat ggctcctagt gtaaaagcag
    17821 gagatgctat tttattttat cgcttatctc agacttataa agtagaagaa gcagttgttt
    17881 atgaggactc taaaacatct ataacgaaag tcggtcggat tattgctcaa gcaggtgatg
    17941 aagtggacct gacagaacaa ggtgaattaa aaattaatgg ccatattcaa aatgaaggac
    18001 taacttttat aaaatcaaga gaagctaatt atccgtatcg aattgctgat aatagttatt
    18061 tgatactcaa tgattactat agccaagaga gtgagaatta cctacaagat gcaattgcta
    18121 aagatgctat aaaaggcacg attaatacac ttattcgatt aagaaaccat taagctaaag
    18181 gcttatttaa aaaaggagag aaacaatgaa attacgtcac ttactattaa cgggagcagc
    18241 cctaactagt tttgctgcta caacagttca cggggagact gttgtaaacg gagccaaact
    18301 aacagttaca aaaaaccttg atttagttaa tagcaatgca ttaattccaa atacagattt
    18361 tacatttaaa atcgaacctg atactactgt caacgaagac ggaaataagt ttaaaggtgt
    18421 agctttgaac acaccgatga ctaaagtcac ttacaccaat tcagataaag gtggatcaaa
    18481 tacgaaaact gcagaatttg atttttcaga agttactttt gaaaaaccag gtgtttatta
    18541 ttacaaagta actgaggaga agatagataa agttcctggt gtttcttatg atacaacatc
    18601 ttacactgtt caagttcatg tcttgtggaa tgaagagcaa caaaaaccag tagctactta
    18661 tattgttggt tataaagaag gtagtaaggt gccaattcag ttcaaaaata gcttagattc
    18721 tactacatta acggtgaaga aaaaagtttc aggtaccggt ggagatcgct ctaaagattt
    18781 taattttggt ctgactttaa aagcaaatca gtattataag gcgtcagaaa aagtcatgat
    18841 tgagaagaca actaaaggtg gtcaagctcc tgttcaaaca gaggctagta tagatcaact
    18901 ctatcatttt accttgaaag atggtgaatc aatcaaagtc acaaatcttc cagtaggtgt
    18961 ggattatgtt gtcactgaag acgattacaa atcagaaaaa tatacaacca acgtggaagt
    19021 tagtcctcaa gatggagctg taaaaaatat cgcaggtaat tcaactgaac aagagacatc
    19081 tactgataaa gatatgacca ttacttttac aaataaaaaa gactttgaag tgccaacagg
    19141 agtagcaatg actgtggcac catatattgc tttaggaatt gtagcagttg gtggagctct
    19201 ttactttgtt aaaaagaaaa atgcttaaat tattattatg atagtaagac tgattaagct
    19261 ccttgacaag ttgataaacg tcattgttct ttgtttcttc tttctttgtt tattgattgc
    19321 ggcacttgga atctacgatg ctttaacagt ttatcaagga gctaatgcta ctaactatca
    19381 acaatataag aaaaagggtg ttcagtttga cgatttatta gctattaatt ctgatgttat
    19441 ggcatggctg actgttaaag gaacgcatat tgattatcca attgtacagg gagagaataa
    19501 tttagaatat atcaacaaat cagtagaagg agagtactcc ttatcaggaa gtgtttttct
    19561 agattatcgt aataaagtaa cttttgaaga taaatactca ttaatctatg cacatcatat
    19621 ggctggaaat gttatgtttg gcgaattacc taactttagg aaaaaatcat tttttaataa
    19681 acacaaagaa tttagtattg aaaccaaaac aaagcaaaaa ctgaaaatca atatttttgc
    19741 atgtatccaa acagatgctt ttgacagttt actgtttaat ccaattgatg ttgatatttc
    19801 tagtaaaaat gaatttttaa accatatcaa gcaaaaatcg gtacagtatc gtgaaatatt
    19861 gacaacaaat gaaagtcgtt ttgttgcctt atcaacctgt gaggatatga caacagatgg
    19921 taggattatc gttattggac aaattgaata attgtaagag aaaggaggtc atatgaaaaa
    19981 atctatatta agaattttag ctattggtta tttacttatg agtttttgtt tgttagactc
    20041 cgtagaggca gagaacctca ctgcaagcat taatattgaa gttattaatc aagttgatgt
    20101 tgctaccaac aaacaatcct ctgacataga tgaaacgttt atgtttgtaa ttgaagcgtt
    20161 agataaagaa agtcctttac ctaattcggt aactacttct gttaagggaa atgggaaaac
    20221 atcatttgaa cagttgactt tttcagaagt tggacaatat cattataaaa ttcatcaact
    20281 gttaggtaag aatagtcaat atcattatga tgaaacagta tatgaagttg ttatttacgt
    20341 attgtataat gagcaaagtg gtgctttaga aactaaccta gtttctaaca aacttggaga
    20401 aaccgaaaaa tcggagctta tttttaaaca agaatatagt gaaaaaacac cggaacctca
    20461 tcaaccagat acaactgaga aggaaaaacc tcagaaaaaa cgtaatggta ttttgccatc
    20521 aactggtgaa atggtgagtt acgtatctgc tttaggaatt gtcttagtgg ctaccattac
    20581 tttgtattct atttataaaa agctaaagac aagcaaatag attcaatttt aagcagagta
    20641 attctccttg aggatttagg aaagatatgt aagaaaaacc aaaaattcct ttacttaatg
    20701 ataaaggaat tttttattgc cccacgacag caaattgttc gtaagcgtcc aataacaagg
    20761 tatattgaaa acctccaaag tttctaattg actctggagg ttttgattat tgacaatttg
    20821 tttgtacttt tttagcccat ggcaaataag cttctagcac ttctctttta gcgagagttt
    20881 cttcattagg aaggcggtct agaaggtagc taatatattt ttcggagttc agtccatgtc
    20941 gcttagccgt ttcaagcaaa ctcatgatga tagctgccgc tttggctcct tcaaaacttt
    21001 gagaaaacaa ccaatttttt cggcccatca ctaaggattt gatagctctc tctgccatat
    21061 tgttagacag aacaatatgg ccatcctcta aaacagttct aaaggttctt tcatgcttga
    21121 gactataagc gatagcgaga cccagcttcg atcctgaaag gacaacttgc tcgcgacacc
    21181 aatcaaagaa ggtggtcatt aatggcgtca agatttcttt gcgtttaacc agacgctctt
    21241 gagggggtaa ctcacaccat tcagcttcta aggcgaacaa cttgtcacag taaacaagtc
    21301 ctttacgccc tagagaagtc ttatcagctt gcttcggggt ggcctcaaag aacttccgcc
    21361 tcacatgtgc ccaacaacca acaagcttgg catgctctaa ttgacgataa gccccatgca
    21421 tatcacaatg cacataacct gagtaatcgc cgagaacctc ttgagtaacg aggccgctac
    21481 gccttttatc atgatggtaa agggtaatcc cttttttctc atgcttacca gacaaaaaag
    21541 tccagtaata ggtcaactga gtgtcacttt caagaactct ataagatgtc tcatccgcat
    21601 gaatgacctc ttgacttaat aagatgtcac gaagcaagtc ataaagaggc tcaaaataat
    21661 actggctaga cttgatatgc cagttagcga tttccttgcg actaatggac agtcctaatt
    21721 tattccaatc ctcttcctgt ctgtagttag gaactttaag ggtgaatttt tgatgaacgg
    21781 tatgggcaat aatagaagcc gaaccaaggc tatgagctaa tggtgcctta ggaacaggcg
    21841 ctttgatgat cttgtcactg aggctattat cgctacaagt ttggcactta taagcgtgtt
    21901 gcacatgatt tatacgtttc aattgtgctg ggataaagac taattcttga cgttgaacca
    21961 ccgagccaat ttctgtcaat gttccatgac aatccggaca agtacagctg tcaccaatta
    22021 attcgtggtg aacaagctca ggttcaaatt gagctagaat agcttgacgt cttcctttga
    22081 ttttcttgcg tctataagta atgtcttctg tttctactgg gcaagtcatc cgcttcttct
    22141 ggtaattggt cctggtcaaa gagactgagt tgaccggtca taggcatagc tttttcagaa
    22201 gatttcccat aaagcttttg agttaaataa gccacttgtt cacgcaggag ggctagctca
    22261 ttagccattg tttcaatggt tttggcttgt gttttaatga ctttatcttg tgaactcatg
    22321 gtcttcctcc tgatattttc ttcattatac ctgaagaaaa gccatgagtt caatagaaat
    22381 catgactttt tgaaattttt atgttaggac taatgaaaaa tcccttcata agccagtcca
    22441 cctgtacagc tgtcaagcat ttgacctcat ccctatttct tggccaggct agtttcccat
    22501 tttcaaagcg tttataaagt aaccagaaac cttgaccatc ccagtaaagc gctttgaacc
    22561 gatcacgtcg cccaccacag aagagataaa cagcacctga aaagagatct aactcatgtt
    22621 gggatttgac aagataggct agggaatcaa tgccttgtcg catatctgtt tttccacaaa
    22681 ccagataaac ttgtcctagg tcatttagcc gaatggtcat cgttcagtac cttatccaaa
    22741 atgctttcca acaagtcctg attgagtgct tgaaataaac taagttccag ttttccaagg
    22801 cgaactttaa gcaagatatc atttctgttt ttttctcaaa tcgacgagat tggggaactt
    22861 ccaaaggaac aatagggtgt gatatttaaa ttatcctccg atagactagt tttactaaca
    22921 gtatactgga ggattgagaa tcacggtaga taccctgtta ttggacgctt acattatttt
    22981 tattgggaac agccattttt ctttacccta cgttaagtag tcagtggaat gctatcgtga
    23041 tcgtcaattg ttatcaactt atcacaaaca agtgactcaa aagaagccct cagaaatgga
    23101 ggaggtgtgg caaaaggcta aggcgtataa cgctaggctt gggattcagc ctgttccaga
    23161 tgctttttca tttagggatg gcattcatga taaaaactac gaaagcctac ttcaaattga
    23221 aaacaatgat attatgggat atgttgaagt gccctctatt aaagtgacgt tacccatata
    23281 ccactatacg acagacgagg tgctaactaa aggagcaggt cacttatttg gaagtgcctt
    23341 accagttggt ggtgacggga ctcacacggt tatttctgct catagaggcc ttccatcggc
    23401 agaaatgttt actaatttga atttagtgaa aaagggagat actttttact ttcgtgtgtt
    23461 aaataaagta ttggcttata aagttgatca gatactaact gttgaaccag atcaagtcac
    23521 atcactttct ggagtcatgg ggaaagatta tgctactttg gttacctgca caccatatgg
    23581 tgttaacacc aagcgtctat tggtccgagg tcatagaatt gcatatcatt ataaaaagta
    23641 tcaacaggct aaaaaagcaa tgaaactcgt ggataaatca agaatgtggg cagaagtagt
    23701 gtgtgctgct ttcggggtgg ttatagctat tattttagtg tttatgtatt cgcgagtaag
    23761 tgctaagaaa agcaagtgat agttaaataa gagattgtaa tagaaactgg tggactttgc
    23821 ccaccagttt tttggggtac ggtggggata tcgtacagaa gagctccttc ggttacttta
    23881 agtataataa aagaaataaa aagagtctga gcaatcatgt tgtcttagac tctgactgta
    23941 ttatgcaaaa gatgatggac tggcagcttc atttactcac cgaattcccc tttataactt
    24001 gggaatgatg ctgctaatgg gttgttttgt ttaatacctg ctataaacgg taatttatcc
    24061 catttatttt tgtaattatc ttcaagctca gttttaaact tccaaaccat tttatgagaa
    24121 aaataagtgt gtgattgacg gccataggta tctactaaaa cgtgagaaaa ttttccgtcg
    24181 ttttttagtt tgtaaaattt atctagagaa ggactccaac cagtataacc agggaattta
    24241 tcaaattctt gattaaaatt tgtttgttgg cttttccaat cataatgcca ttgtgtatta
    24301 ttgcgtgttt cagaacgaac tgtaccgtcc caataatgat gctcataagt tatctctgta
    24361 ggagctttta cacccttagg aagaaaaact gagaaatcag ggtaaaccca ttgttcttta
    24421 gctgaattaa aggttacagt ccattcaata tttttatcgc tatcgaccaa ttttttagta
    24481 tagtcgacat agttatcaac atagtaattt ccctgccagt aaaggtgcac tttacctttc
    24541 tcatcaatgt tatcatgggc atgtactgat ttatacgaac taaacagcat cgaagctgct
    24601 agtagtgaag tgaagagata tttattacat cgtttcatat catgaaaaac cttt
//
LOCUS       DAXWIQ010000025        24642 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000025 DAXWIQ010000000
VERSION     DAXWIQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24642
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.025"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(178..2442)
                     /gene="glgP"
                     /locus_tag="ACGEUI_001050"
     CDS             complement(178..2442)
                     /gene="glgP"
                     /locus_tag="ACGEUI_001050"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HFF8165892.1"
                     /translation="MTRFTEYVETKLGKSLTQASNEEIYLSLLNFVKEEASHKAKNSA
                     KRKVYYISAEFLIGKLLSNNLINLGIYKDIKEELAAAGKSIAEVEDVELEPSLGNGGL
                     GRLASCFIDSIASLGINGEGVGLNYHCGLFKQVFKHNEQEAEPNFWIEDDSWLVPTDI
                     SYDVPFKNFTLKSRLDRIDVLGYKRDTKNYLNLFDIEGVDYGLIKDGISFDKTQIAKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLIIDEAIERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGFDFDEAVAVVKNMVGYTNHTILAEALEKWPTAYLNEVVPH
                     LVTIIEKLDALVRSEVSDPAVQIIDESGRVHMAHMDIHFATSVNGVAALHTEILKNSE
                     LKAFYDLYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLMAFA
                     DDKAVHAKLAEIKFNNKLALKRYLKDNKDIELDEHSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKKGNLPKRKITVIFGGKAAPAYIIAQDIIHLILCLSELINNDPEVSPYLNV
                     HLVENYNVTVAEHLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGMENIYTFGKDSDTIINLYATASYVAKDYYDNHPAIKAAVNFIISPELLAFGNEE
                     RLDRLYKELISKDWFMTLIDLEEYIEVKEKMLADYEDQDLWMTKVVHNIAKAGFFSSD
                     RTIEQYNEDIWHSR"
     gene            complement(2477..3970)
                     /gene="malQ"
                     /locus_tag="ACGEUI_001051"
     CDS             complement(2477..3970)
                     /gene="malQ"
                     /locus_tag="ACGEUI_001051"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFF8165893.1"
                     /translation="MNKRASGILMHISSLPGKFGIGTFGKSAFEFVDFLAETKQTYWQ
                     ILPLTTTSFGDSPYQSFSAIAGNTHFIDFELLVDDELLEAADLCDITFGTNPEAVDYA
                     QLFQVRRPLLEKAVRAFVAEQENVCKLEAFETASSWLTDFAEFMALKEYFNNKALQDW
                     DDETVIKRQEDSLNNYRELLAKKITYHKVCQYFFYQQWSALKTYANHKGIEIIGDMPI
                     YVSADSVEVWTMPELFKVDSDKKPLFIAGVPADGFSEDGQLWGNPTYNWSAHEKSNFA
                     WWIYRIQESFKLYDQLRIDHFKGFSDFWEIPAGDKTARNGHWASAPGIALFSAVREAL
                     GELPIIAENLGYIDEKAEQLLASTGFPGMKILEFGLFDITSQSIDLPHYYDRNCVAYT
                     GTHDNEVVNGWYDNLSEEQVHFVNNYLHKHADESITKAMLRTIFASVCDTAILCIQDL
                     LDKDGKSRMNMPNTIGGNWQWRMLDGELNQDHKDYLIYLTDLYGRAR"
     gene            complement(4085..5104)
                     /locus_tag="ACGEUI_001052"
     CDS             complement(4085..5104)
                     /locus_tag="ACGEUI_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8165894.1"
                     /translation="MVTIKDVAQKAGVNPSTVSRVLKDNRSISMKTKEKVRKAMADLG
                     YVPNVAAQILASGLTHNIGLVFPPITTSDRLSEPFFMEILSTITNEAKESHFTVSIAT
                     GISLDDLIEQVKLMHLQKRVDGFIILYSDQDDPVKKYLMTNNLPFVIVGAPQGDTNKT
                     TYIDNDNQLMAKTAVEHLYQKGHHNILFITDDLLSEVTSERYLGYLKGCFKRSLKTKP
                     MLLFDRKDPVSVEILMETISSFKATALVVVGDVLAIRMVQLLSFYDIKVPDDMSIITF
                     NNSNYAKLIHPYLTTFDINVENLGKMSFKQLLDIINSNEQNLSQTIFVPFSLKQRESV
                     RDISH"
     gene            5350..6597
                     /locus_tag="ACGEUI_001053"
     CDS             5350..6597
                     /locus_tag="ACGEUI_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8165895.1"
                     /translation="MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLW
                     VPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADVFSLPHDQLGQ
                     LVESGTIQEVPEKYNKEIAATSTDQALVGAQYKGKTYAFPFGIESQVLFYNKSKLAAE
                     DVTSYDTITTKATFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAV
                     LKWIADQASNKGFVSLDANNVMSKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYP
                     KVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRN
                     IVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFN
                     GKIKENDYLTKLQQFDKDIAATK"
     gene            6871..8232
                     /locus_tag="ACGEUI_001054"
     CDS             6871..8232
                     /locus_tag="ACGEUI_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8165896.1"
                     /translation="MTQSNLTPNISVIEALKRGSWDIKLSSIIMGFANFANKQFIKGI
                     LFLISELIFLVAFVSQIIPAIRGLVTLGTQTQGMTTKTIDGINIQVAVDGDNSMLMLI
                     FGLASLIFCLVFAYIYWCNLKSARNLYLFKQKGQKIPSFKEDLATLTNGRFHMTLMAI
                     PLIGVLLFTILPLIYMICLAFTNFDHNHLPPKSLFDWVGLANFGNVLSGRMAGTFFPI
                     FSWTLIWAVFATVTNFFFGIILALLINTKGLKWKKMWRTIFVITIAVPQFISLLIMRN
                     LLNDEGPLNALLNKIGLINGSLPFLSDPLWAKFSIIFVNMWIGIPFTMLIATGIIMNL
                     PSEQIEAAEIDGASKFQVFKSITFPQILLIMTPNLIQQFIGNINNFNVIYLLTGGGPT
                     NSEYYQAGTTDLLVTWLYKLTVTAADYNLASVIGILIFTVSAIFSLLAYTRTASYKEG
                     AAK"
     gene            8232..9068
                     /locus_tag="ACGEUI_001055"
     CDS             8232..9068
                     /locus_tag="ACGEUI_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFF8165897.1"
                     /translation="MKNKRRFQLGLVYATLIILSIIWLFPIAWVILTSFRSEGTAYVN
                     YFIPKTFTLNHYINLFTNETFPFGKWFMNTLIVATFTCIISTFITVAIAYSLSRIKFK
                     FRNGFLKLALILNMFPGFMSMIAIYYILKALGLTQTLTALVLVYSSGAALGFYIAKGF
                     FDTIPYSLDESAMIDGATRMDIFFKITLPLAKPIIVYTALLAFMGPWIDFIFAQVILG
                     DATSKYTVAIGLFSMLQPDTINNWFMAFTAGSVLIAVPITLLFMFMQKYYVEGITGGS
                     VK"
     gene            9226..10023
                     /locus_tag="ACGEUI_001056"
     CDS             9226..10023
                     /locus_tag="ACGEUI_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8165898.1"
                     /translation="MTSKITIKDIAREAGVSVATVSYVLNQRNDQKISEETRNKVLHI
                     SNLLNYTPNQAARSLVTQQSNSVVLYYKYADSILARAEQLHTISFLSRLLHEQDYQLV
                     CLNEDSTEKFDWADLILTLDVDNDTFYKIGKLNFKPLIAIDSFVNDPLFFQINTDYAA
                     LKDQANQFFKDEPYTLLSLPITNRERYEHVSAFFPSLHIVNDLRELTYFEKENLLLID
                     ESLDQFFVNSSQKLCCPRFSPKKGQVILETIEWAKKRQPDKKHNLLV"
     gene            complement(10139..10954)
                     /locus_tag="ACGEUI_001057"
     CDS             complement(10139..10954)
                     /locus_tag="ACGEUI_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFF8165899.1"
                     /translation="MPDKKQFPISYISHCLSPRAMFASRAQFTWWQNLLIIVFLNALI
                     MIPVTLHYANMTTYPLERIVTKSLSPITDKTYQALTQGKIEKDTFQGQSLIRRDGELV
                     LAVLPTKVDLEQLASESTRQIIVTKKEWRFVTPDGKELRAHVRGQQQSLADLTTVKAV
                     KDFVNQQWYDSNKASVLGFLLLTFVLMVCVGTLIVIGLGAFFLTLTKRSRLFMIRNFS
                     EGLGLMVNCLAWPSLLAIALSFFIQDPVLIMNCQVFGTLLMLTWVFYKTQFRD"
     gene            complement(10976..11836)
                     /locus_tag="ACGEUI_001058"
     CDS             complement(10976..11836)
                     /locus_tag="ACGEUI_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFF8165900.1"
                     /translation="MRKTYVSISMKRKRFFTQLLTYLYLISLVIVILFPILVTVSSAF
                     RPGNTTAFSFHFDGPWTLSNFKTLFQDTLYLRWYWNTLIVAFFTMLIQVTVITLTGYA
                     YSRYNFFGRKKSLIFFLVVQMVPTMAALTAYFVMAWLFNALNQYWFLILIYVGGGIPM
                     NAWLMKGYFDTVPYDLDESAKLDGSGHFRTFYQIVLPLVRPMIAVQSLWAFMGPFGDF
                     MLAKFLLRAQENYTVAVGLQSFITNDARNPKVTLFAAGAILIAVPISVLFFFLQKNFV
                     SGLTSGGTKG"
     gene            complement(11833..13140)
                     /locus_tag="ACGEUI_001059"
     CDS             complement(11833..13140)
                     /locus_tag="ACGEUI_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8165901.1"
                     /translation="MVENQRQQKPNKAMWLSVIPGLGQLYNKQIVKGGVLLVVFFLEL
                     LEIVVLGIPALTGLYSLGSVPMQDHSLFMLIKGAMQLITLVLFGIFHLVAMRDAKLVA
                     HQMNEGKKVPVTAKETLEAIYEKGFPYLLIIPAYLAMAFAIIFPVLVTLLIAFTNYDF
                     RHIPPYRLLDWVGLKNFLNILELSTFKTAFTSVLSWTLIWTLAATSLQIVIGIATAVI
                     ANQSFIRGKRLFGVIFLLPWAVPAFISIMSFGNFFNDSIGAMNVQVLPFIEKFLPFVD
                     FGIVSWKTDPFWTKVAVIMVQGWLGFPYIYILVSGILQAIPADLYEAATVDGATVLQK
                     FRHITMPMIFAVAAPTFISQYTFNFNNFSIIYLFNNGGPGTVGGGAGTTDILISWIYK
                     LTTQTSPQFSMASAVTLIISVIVISVSLITFKKFKAFDMEERS"
     gene            complement(13215..15350)
                     /gene="amyA"
                     /locus_tag="ACGEUI_001060"
     CDS             complement(13215..15350)
                     /gene="amyA"
                     /locus_tag="ACGEUI_001060"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase AmyA"
                     /protein_id="HFF8165902.1"
                     /translation="MRELHIKTYKLLTKSAVLLGLISFPLTVSAADNASVTNKADFST
                     DTIYQIVTDRFNDGNTSNNGKTDVFDKNDLKKYHGGDWQGIIAKIKDGYLTDMGISAI
                     WISSPVENIDSIDPSNGSAAYHGYWAKDFFKTNQHFGTEADFQQLVKVAHQHHIKVVI
                     DFAPNHTSTAEKEGTTFKEDGALYKNGKLVGKFSDDKDKIFNHESWTDFSTYENSIYH
                     SMYGLADLNNINPKVDQYMKEAIDKWLDLGVDGIRVDAVKHMSQGWQKNWLSHIYEKH
                     NVFVFGEWFSGHTDDDYDMTTFANNSGMGLLDFRFANAIRQLYTGFSTFTMRDFYKVL
                     ENRDQVTNEVTDQVTFIDNHDMERFATKVANNQTAVNQAYALLLTSRGVPNIYYGTEQ
                     YATGDKDPNNRGDMPSFNKESQAYKVISKLAPLRKQNQALAYGTTEQRWISDHVLVFE
                     RKFGNHVALVAINRDQTNGYTITNAKTALPQNSYKDKLEGLLGGQELIVGADGTISSF
                     ELGAGQVAVWTYEGEDKTPQLGDVDASVGIAGNKITISGQGFGNSKGQVTFGEISAEI
                     LSWSDTLITLKVPTVPANYYNISVTTADKQTSNSYQAFEVLTDKQIPVRLLINDFKTV
                     PGEQLYLMGDVFEMGANDAKNAVGPLFNNTQTIAKYPNWFFDTHLPINKEIAVKLVKK
                     DSIGNVLWTSPETYSIKTGHEAQTITIKK"
     gene            complement(15376..17079)
                     /locus_tag="ACGEUI_001061"
     CDS             complement(15376..17079)
                     /locus_tag="ACGEUI_001061"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFF8165903.1"
                     /translation="MNVAGMLHIPDSRYCFAISQKELVIRLRVAKEDRGITVNLVHGP
                     KYGYHEFQEELEMSVAYTDHTHAYYETTLTLDDVRLAYIFKIEEEGQTYYFSEDGLTT
                     SYDFSNGFYNFFQMPYINAVDVHQVIPWTKDAVFYQIFVDRFNRGDWEKGDSYVNMGW
                     LEKPTPKSFAGGDLKGITEKLDYLKDLGITVIYLTPIFQSISNHKYDISDYYAIDPQF
                     GTKYDLQELIDLAHKMGIKIILDAVFNHASSDAVEFQDVLRYGKESKFFDWFMTHDEH
                     PSMDLVNYETFAGCNYMPKWNTSNRDVQDYLIEIGRYWIKEFCIDGWRLDVSDEVSHD
                     FWRRFRQAVKAEKADAILIGENWHDAYPYLAGDQYDGIMNYAFTKACLDYFAFETMTS
                     KQLAEKLSNILMRNTWQVNRMNLNLLDSHDTHRFFTQVKGSKDKLLAALALLFIFMGI
                     PCLYYGTEVAMEGGYDPDSRRGFPWDDAERDKDFFSKVKAIIALRQQPALQEGAISID
                     YDEDCLILKRSLGEQSISLVLNNGESAYSIKEGGQILTSNGVTKNKKQLLQDGYIVFS
                     N"
     gene            complement(17245..18504)
                     /locus_tag="ACGEUI_001062"
     CDS             complement(17245..18504)
                     /locus_tag="ACGEUI_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8165904.1"
                     /translation="MSWNWKKTSVLGTLSLASVLPLTACVSGGGKGVKETDGKTIVVS
                     VDEGYVDYIKSIKGEFEKEHKVTVKVKKEGMMDTLDKLSTDGPTGASPDVFLAPFDRV
                     GGLGTEGQIAEVTLGNSKEFDDTVKKLVTIDGKTYGAPDVIETLVTYYNKDLVPQAPK
                     SFTELEVLQKDSKFAFASEPGKSVGFLAKWTDFYYGYGLIAGYGGYIFGDKGTKPSDL
                     GLGNDGTVEGLNYAKQWYGTWPQGMQDTKKAGDFITEQFISKKAGVIIDGPWAASSFK
                     DAGVNFGVMEIPTLTNGKKYQPFAGGKAWVISNYSKGKTTAQKFLDYVTNAENQKRFY
                     DKTQEIPANLTARNYASKEGNELTKAVISQFESAQPMPNIPEMAEVWEPGANMFFNVA
                     SGKEEASKAAKEAAKTIKEAIEQKYAE"
     gene            18932..19036
                     /locus_tag="ACGEUI_001063"
                     /pseudo
     CDS             18932..19036
                     /locus_tag="ACGEUI_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19119..20102)
                     /locus_tag="ACGEUI_001064"
     CDS             complement(19119..20102)
                     /locus_tag="ACGEUI_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8165905.1"
                     /translation="MVIIAVVIFFGVILGILIVEYGQKRSFSSWLVEKMFAYRKYKLS
                     KKVDNYKGVETQRNRNIPYHLDNPEEIVGFSTQEYDVNGMQVFIINDQKQSHQPVIYY
                     LYGSVYLRRPEKLHFKTVKHLIQLTNAKVVFPNFPKVPYATYQDVYPKLVEAYQQFCL
                     SNGPSQVSVIGDSSGGGMALAFAKYIRDTKTAPQPKQLILISPWLDVSFKNQAYKDFE
                     AEEAYLDARRLRDIGELWAGGQGNMQLPYVSPLYGDYEDLPEITTFVGTKEIFYPDIM
                     SLHDQLTRQRIANQLIIAEKMIHAYVIFPIREAKQARKQIAELVLKTVEHT"
     gene            complement(20133..21383)
                     /gene="dltD"
                     /locus_tag="ACGEUI_001065"
     CDS             complement(20133..21383)
                     /gene="dltD"
                     /locus_tag="ACGEUI_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFF8165906.1"
                     /translation="MLKRLWLILGPLLIAFVLVVITIFSFPTQLDHSIAQEKANAVAI
                     TDSSFKNGLIKRQALSDETCRFVPFFGSSEWSRMDSMHPSVLAERYKRSYRPFLIGKR
                     GSASLSHYYGMQQITNEMQKKKAIFVVSPQWFTAQGINPSAVQMYLSNTQVIEFLLKA
                     RTDKESQFAAKRLLELNPGVSKSNLLKKVSKGKSLSRLDRAILKCQHQVALREESLFS
                     FLGKSTNYEKRILPRVKGLPKVFSYKQLNALATKRGQLATTNNRFGIKNTFYRKRIAP
                     KYNLYKNFQVNYSYLASPEYNDFQLLLSEFAKRKTDVLFVITPVNKAWADYTGLNQDK
                     YQAAVRKIKFQLKSQGFHRIADFSKDGGESYFMQDTIHLGWNGWLAFDKKVQPFLETK
                     QPVPNYKMNPYFYSKIWANRKDLQ"
     gene            complement(21376..21615)
                     /gene="dltC"
                     /locus_tag="ACGEUI_001066"
     CDS             complement(21376..21615)
                     /gene="dltC"
                     /locus_tag="ACGEUI_001066"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFF8165907.1"
                     /translation="MSIEETVIELFDRLFMEDVSEMMDEDLFDAGVLDSLGTVELIVE
                     LESTFNIKVPISEFGRDDWNTVTKIVQGVEELQHA"
     gene            complement(21633..22889)
                     /gene="dltB"
                     /locus_tag="ACGEUI_001067"
     CDS             complement(21633..22889)
                     /gene="dltB"
                     /locus_tag="ACGEUI_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFF8165908.1"
                     /translation="MMMFFSHIPYMEPYGNPIYFVYLILAFLPVIIGIFKQKRLSTYE
                     TLVSLVFILFMFGGDHYQQLVAFLFYLLWQIISVFAYQKYRENANSAGVFYLAIAMAL
                     FPLIWVKVAPLTGPSSQTLFSFLGISYLTFKSIGMIIEMRDGTLQEVRLPDFIRFMIF
                     FPTFSSGPIDRFRHFQEDYHKLPERDDYFAMLNKAVMYLMLGFLYKHIISYCLGGILL
                     PLLENKALMVGGYFNKETILVMYVYGLNLFFDFAGYSMFAIGISYLLGIRTPENFNMP
                     FLSASLKDFWNRWHMSLSFWFRDYVFMRLVHLLIKHKTFKNRNVTSGVAYLVNMLVMG
                     FWHGLTWYYIAYGLFHGIGLIINDAWIRKKKEINRHRKKKGLSPLFQSRAFHVLCIVV
                     TFHVVMFSLLLFSGFLNDLWFNRPLH"
     gene            complement(22886..24424)
                     /gene="dltA"
                     /locus_tag="ACGEUI_001068"
     CDS             complement(22886..24424)
                     /gene="dltA"
                     /locus_tag="ACGEUI_001068"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFF8165909.1"
                     /translation="MIKDMIDSIEQFAQTQADFPVYDCLGERRTYGQLKRDSDSIAAF
                     IDSLALLAKSPVLVFGAQTYDMLATFVALTKSGHAYIPVDVHSAPERILAIIEIAKPS
                     LIIAIEEFPLTIEGISLVSLSEIESAKLAEMPYERTHSVKGDDNYYIIFTSGTTGQPK
                     GVQISHDNLLSFTNWMIEDAAFDVPKQPQMLAQPPYSFDLSVMYWAPTLALGGTLFAL
                     PKELVADFKQLFTTIAQLPVGIWTSTPSFADMAMLSDDFCQAKMPALTHFYFDGEELT
                     VSTARKLFERFPSAKIINAYGPTEATVALSAIEITREMVDNYTRLPIGYPKPDSPTYI
                     IDEDGKELSSGEQGEIIVTGPAVSKGYLNNPEKTAEAFFTFKGQPAYHTGDIGSLTED
                     NILLYGGRLDFQIKYAGYRIELEDVSQQLNQSPMVASAVAVPRYNKEHKVQNLLAYIV
                     VKDGVKERFDRELELTKAIKASVKDHMMSYMMPSKFLYRDSLPLTPNGKIDIKTLINE
                     VNNR"
     gene            complement(24436..24579)
                     /locus_tag="ACGEUI_001069"
     CDS             complement(24436..24579)
                     /locus_tag="ACGEUI_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFF8165910.1"
                     /translation="MIKNKSRMRGIAVFIGKTILFYLILMLLVYFFGYLGHGQSNFIY
                     NEF"
BASE COUNT         7815 a         4966 c         4153 g         7708 t
ORIGIN      
        1 aactttagtg tagtttctta taattacctc catagatcgc taaacagact tgctttacgc
       61 ttaatgtaat aattaaccta agtctgaagt aaaatcataa gacagcagaa actactgtct
      121 tataatttag tttaagcatg ttacggtacc gctaccccac attgcaatta ttatttttta
      181 tcgtgaatgc caaatatctt cgttatactg ctcaatggta cggtcagatg agaagaatcc
      241 agcttttgcg atgttatgga ccacttttgt catccataaa tcttggtctt catagtctgc
      301 taacattttt tctttcactt caatgtactc ttcaaggtca atcaaagtca tgaaccagtc
      361 ttttgaaatc aattctttat aaagacgatc aagacgttct tcgttgccaa atgctagcaa
      421 ttctggactg ataataaagt tcactgctgc tttaatagca gggtggttat catagtaatc
      481 ttttgctaca taagaagcag tcgcataaag gttgatgatg gtgtcagaat ctttaccaaa
      541 ggtatagata ttctccatgc ctgcaagctc agcgatttct acgttagcac cgtccattgt
      601 accaagtgtt aaagcaccgt taagcatgaa tttcatatta ccagttccag aagcttcttt
      661 ggatgctagt gaaatttgct cagaaatatc agttgctgga atcaagtgct ctgctactgt
      721 cacgttgtaa ttttcaacta gatgcacatt aaggtatggg cttacttcag ggtcattgtt
      781 aatcaattca gacaagcaaa ggatcaagtg aatgatgtct tgcgcaataa tgtaagcagg
      841 cgctgcttta cctccaaaga taacagtgat tttacgtttt ggaaggttgc cttttttaat
      901 ttccaaatat ttgtgaatca cgtaaagagc attcatctgt tgacgtttgt actcgtggaa
      961 acgtttgatt tgggtatcaa taatagaatg ttcatcaagc tcaatgtctt tattgtcttt
     1021 aaggtaacgt ttaagagcta atttgttgtt gaatttgatt tcagccaatt tagcatgaac
     1081 agctttgtca tctgcaaagg ccatcaattt ttctaatttt gttgcgtcag tcaagtactc
     1141 atcgccaata agttctttaa tgtaatcagc caagtcttgg ttagcaaatt ctagccaacg
     1201 acggaaagta atcccgttgg ttttgttgtt gaatttttct gggtaaaggt catagaaagc
     1261 ttttaattcg ctgtttttca agatttctgt gtggagtgct gctaccccat tgacacttgt
     1321 tgcaaaatga atatccatat gggccatgtg cacacgacct gattcgtcaa taatttgaac
     1381 agctggatca gacacttctg aacgaacaag agcatccaat ttttcaatga tggttaccaa
     1441 gtgtggcact acttcgttta agtaagcagt tggccatttt tcaagggctt ctgcaagaat
     1501 agtgtggtta gtgtaaccaa ccatattttt cacaacagct accgcttcat caaagtcaaa
     1561 gccatgtttt tcagtcaaga gacgaattaa ttctgggatg accattgatg gatgcgtgtc
     1621 attaatttgc acgtaagcgt agtctgcaag gtcatgaagg tttgaaccac gttcgatagc
     1681 ttcatcaatg attaattgcg ctgcatttga caccataaag tactgttggt aaatacggag
     1741 caattcccca tttttgtcag aatcgtctgg gtagaggaac aaggtcaagt ttttagcaat
     1801 ttgcgtttta tcaaaagaaa tgccgtcttt gattaacccg taatcaacgc cctcgatatc
     1861 aaacaagtta aggtagtttt tagtgtcgcg tttgtaaccc aaaacatcaa tacgatcaag
     1921 acgagatttc aaggtgaagt tcttaaaagg cacatcgtaa gaaatgtctg ttggaaccaa
     1981 ccatgagtca tcttcaatcc agaagtttgg ctcagcttct tgctcattgt gtttgaagac
     2041 ttgcttaaac aacccacaat gatagtttaa accaacccct tcaccattaa tcccaagaga
     2101 tgcaatagag tcaataaaac atgaagcaag gcgtcctaaa ccaccattac ctagtgatgg
     2161 ttctaattca acatcttcga cttctgcgat agacttgcca gcagctgcca attcttcttt
     2221 gatgtccttg taaattccta ggttaatcag gttgtttgat agtaatttac caatcaaaaa
     2281 ctctgctgag atatagtaaa ctttgcgttt agcagaattt ttagccttgt ggctagcttc
     2341 ttctttgaca aagtttaata atgatagata aatctcttca ttgctggctt gcgtaagtga
     2401 ttttcctagt ttagtttcaa catattctgt aaagcgtgtc atgtcttcct cttttcaata
     2461 atgtaacatt taaatattat cttgcgcgac cataaagatc tgttaaataa ataaggtaat
     2521 ctttgtgatc ttggttcaat tcaccgtcaa gcatacgcca ttgccaatta cctccaattg
     2581 tatttggcat attcatacga ctcttaccat ctttatctaa taaatcttgg atgcaaagaa
     2641 tggctgtatc acaaacagaa gcaaagattg ttcttagcat ggcttttgta atactttcat
     2701 ctgcatgttt gtgaagatag ttatttacaa agtgtacttg ttcttcacta agattgtcat
     2761 accatccgtt aaccacctcg ttatcatgag tccctgtgta agcgacacaa ttacggtcat
     2821 agtaatgtgg taaatctata ctttgactcg ttatatcaaa taaaccaaat tctaatatct
     2881 tcatgccagg aaagcctgtt gatgctaata gctgctctgc tttttcatca atataaccca
     2941 ggttttctgc gatgataggg agctctccta aggcctcacg aaccgcagaa aataatgcta
     3001 tacctggggc tgaggcccaa tggccatttc tagcagtttt atcaccagca ggaatttccc
     3061 aaaaatctga gaagccttta aaatgatcga ttcttagctg atcatatagt ttgaaacttt
     3121 cttgaatacg ataaatccac catgcaaaat tagacttttc atgagcagac caattgtagg
     3181 ttggatttcc ccacagctga ccatcttcac taaagccatc tgctggtaca cctgcaataa
     3241 acaatggctt tttatcacta tctactttaa atagctcagg catcgtccaa acttctacac
     3301 tgtccgcaga aacataaata ggcatatcac caataatttc tatcccttta tggttagcgt
     3361 aagttttcaa ggctgaccat tgttggtaaa agaagtattg acagacttta tgataggtaa
     3421 tcttttttgc aagtaactca cggtaattat taagtgagtc ttcttggcgt ttaatgacag
     3481 tttcgtcgtc ccaatcttgt aaggctttat tgttaaaata ttcttttaaa gccataaatt
     3541 cagcaaaatc agttaaccag ctagaagctg tttcaaaagc ttctaattta cacacatttt
     3601 cttgttcagc aacaaaagct ctcactgctt tttctaaaag tggacgtcta acttgaaaaa
     3661 gctgagcata gtctactgct tcaggatttg tgccgaatgt aatatcacac aaatctgctg
     3721 cttctaaaag ttcatcatct actagcaatt caaaatcaat gaaatgtgtg ttcccagcaa
     3781 tagctgaaaa tgactgataa ggagaatctc caaaactcgt cgttgttaaa ggcagaattt
     3841 gccaataggt ttgtttcgtc tctgctagaa aatcaacaaa ttcaaaagct gattttccaa
     3901 aagtgcctat cccaaatttc ccaggcaggg aactaatatg cattaagata ccgcttgcac
     3961 gtttgttcat gatttttctc cttgtgaaat ctattcatga tgagattata acgcaagcgc
     4021 ttgcgtaagt caaccagttt cctaaaaaaa gtaacattcc ttaaaaatgc tacttttttt
     4081 gttcttaatg actaatatca cggacgcttt ctctttgttt taaactaaaa ggaacaaaaa
     4141 tagtctgact aagattctgt tcattggaat tgataatatc aagtaattgc ttaaaagaca
     4201 tttttccaag attttcaaca tttatatcaa atgtcgttaa ataagggtga attaatttag
     4261 cgtaattaga attattaaaa gtgataattg acatatcatc tggcactttt atatcataaa
     4321 aagataataa ctgtaccata cgaatagcca gaacatcacc aacaacaacc aaggcagttg
     4381 ctttaaagga agatatagtt tccatcaata tttctacgct aacaggatcc ttacgatcaa
     4441 ataacagcat tggctttgtt tttaaagatc gcttaaaaca acccttcaaa tagccaagat
     4501 aacgctcaga agttacttct gatagtaaat catctgttat aaataatatg ttgtgatggc
     4561 ctttttgata aaggtgttct actgctgttt tagccattag ctgattatca ttatcaatat
     4621 aagtggtttt attagtgtca ccttgtggag cacctacaat gacaaatggt aagttatttg
     4681 tcatcaaata ttttttgaca ggatcatctt gatcagaata aagaataata aaaccatcaa
     4741 ctcgtttttg aagatgcatc agtttcactt gctctatcag gtcatcaaga gatatacctg
     4801 tagcaatcga cacagtaaaa tgagattctt tagcttcgtt agtaatagtc gacaaaattt
     4861 ccataaagaa aggttcactt aatctatcgc tagttgtaat aggaggaaaa acaagtccta
     4921 tattgtgtgt caaaccactc gctagaatct gtgctgcgac atttggaaca tatcccaaat
     4981 ctgccatcgc ttttctaacc ttctcttttg tcttcataga aatagatcga ttatctttta
     5041 ataccctact caccgtcgaa gggttaacac ctgccttttg agcgacatct tttattgtta
     5101 ccatattaac ctcgttacta tatttaccaa agataaacct tatcatgggt ttgtcatgta
     5161 tttcgtttcc attatacaat tatctttcaa aaaatcaact tcaatcaaga ttcccttgtc
     5221 attttaactc aaattctcta aactattttt ttaaaaaaaa cgaccttttt tttaaaaaaa
     5281 ggcttgactt tttctgcaag cggttgcata atattaagtg taagaaatat atcttaggag
     5341 gaaatagcta tgaaatcatg gcaaaaagtt atcgtcggcg gagcaagttt gacacttgca
     5401 agtaccttgt tagtgggatg tggatcaggt tcaaaagata aaaaggaagc tggcgctgac
     5461 tcaaagacta ttaaactttg ggtaccgact ggctctaaaa aatcatatgc agataccatt
     5521 gcaaaatttg aaaaagattc tggttacacc gttaaagtgg ttgaatcaga agatccaaaa
     5581 gcacaagaaa aaatcaaaaa agatgcctca actgctgccg atgtcttttc acttcctcat
     5641 gatcaacttg gtcaacttgt agaatctggt actattcaag aagttccaga aaagtacaat
     5701 aaagaaattg ctgctacttc tactgatcag gcgctcgtcg gtgcacagta caaagggaaa
     5761 acatatgctt tcccatttgg tattgaatca caagttcttt tctacaataa atcaaaatta
     5821 gctgctgaag acgttacttc atatgataca attactacaa aagcaacttt tggtggtacc
     5881 tttaaacaag ctaacactta cgctactggt ccattattca tgtcagttgg taacacttta
     5941 tttggggaaa acggtgaaga tgtaaaaggt actaactggg gcaatgaaaa aggcgctgct
     6001 gttcttaaat ggatcgcaga ccaagcttca aataaaggtt ttgttagctt agatgctaac
     6061 aacgttatgt ctaaatttgg tgacggttca gtagcttcct ttgaatcagg accgtgggac
     6121 tatgaagctg ctcaaaaagc tatcggtaaa gaaaaccttg gtgttgctat ttatccaaaa
     6181 gtaactattg gaggcgaaac ggttcaacaa aaagcattct tgggtgtaaa actttacgca
     6241 gtcaaccaag caccagctaa aggtgataca aaacgtatcg cagctagcta caaattggca
     6301 tcctacttga ctaatgctga aagccaagaa aaccaattca aaactcgtaa cattgtccca
     6361 gccaacaaag aagttcaatc ttcagaagct gttcaatcaa acgaacttgc taaaaccgtt
     6421 atcactatgg gttcttcttc agactacact gtcgtaatgc caaaacttag ccaaatgggc
     6481 acattctgga ctgaaagtgc tgctattctt agtgatgcct tcaacggtaa aatcaaagaa
     6541 aacgactacc ttactaagtt gcaacaattc gacaaagata tcgctgcaac aaaataatat
     6601 gatctcatgt catcactgct atactaaaac aagcacaagg taagtacttc taaaaagttc
     6661 tttccactta ccttatgaag agtggaacta ggctccatca cattttattc aagcttagtc
     6721 tcactcttac ttgtttttgt tttactagta gcttagtgct acttaaattt ttaactcttt
     6781 aaaagtttac gtaacactta gctatttcat cctaacaaga gattgaatct gaactaccac
     6841 tctagtatcc taaaataaag gagaatctat atgactcagt caaacttgac accaaatata
     6901 tctgtaatag aagccttaaa aagaggcagt tgggatatta aactttcgag tattatcatg
     6961 ggatttgcca actttgctaa caaacagttt atcaaaggaa tcttattttt aataagtgaa
     7021 cttatctttc tagtagcttt tgtctcccaa atcatccctg ctatccgtgg ccttgtcact
     7081 cttggtactc aaactcaggg aatgactaca aaaactattg atgggattaa catccaagtt
     7141 gctgttgatg gcgataactc tatgttaatg cttatttttg gtttagcatc actcattttc
     7201 tgcctagtct ttgcttacat ttactggtgt aatctcaaaa gtgctcgtaa cctttatctt
     7261 ttcaaacaaa aaggtcagaa gataccaagc ttcaaagaag atcttgccac acttacaaat
     7321 ggtcgctttc atatgacttt aatggctatc ccactgattg gtgtgctttt attcaccatt
     7381 cttcctttga tttacatgat ctgtttggcc tttaccaatt ttgatcataa tcacttgcct
     7441 ccaaaatctt tatttgattg ggtgggactt gctaactttg gaaatgtttt aagcggccgt
     7501 atggcaggaa ccttttttcc tatcttctca tggactttga tttgggctgt ttttgccaca
     7561 gtaactaatt tcttctttgg tattattctt gctttgttaa tcaataccaa aggattaaag
     7621 tggaaaaaaa tgtggcgaac catctttgtt atcaccattg cagttccaca attcatctca
     7681 ttattgatta tgcggaactt actcaatgac gaaggcccac taaatgctct tctcaataaa
     7741 atcggcttga ttaatggctc actgccattt ttatctgatc ctctttgggc aaaattctca
     7801 atcatttttg tcaacatgtg gatcggtatt ccatttacca tgctgattgc gactggtatt
     7861 atcatgaacc ttccaagtga acaaattgag gctgctgaaa ttgatggcgc tagcaaattc
     7921 caagtcttca agtctatcac gttccctcaa attctcttga tcatgacacc aaacttgatt
     7981 caacaattta tcggaaatat caataacttt aacgtcattt acctccttac tggtggtggt
     8041 ccaacaaatt cagaatacta tcaggcagga acaacagact tgctggtcac ttggctttat
     8101 aaattaaccg tcacagctgc tgactacaat ttagcttctg ttatcggtat cttaatcttt
     8161 acagtttcag ctatctttag cttacttgct tatacaagga cagcatccta caaggaagga
     8221 gcggctaaat aatgaaaaac aaacgtcgat ttcaacttgg acttgtttat gctactttaa
     8281 tcatcctctc aatcatttgg ctcttcccaa tcgcttgggt tattcttact agcttccgta
     8341 gcgaaggaac tgcttacgtc aattatttta ttcctaagac ttttacatta aatcactaca
     8401 ttaatctttt tactaatgaa acctttcctt ttggaaaatg gtttatgaat actcttattg
     8461 tagccacttt tacatgtatt atttcaacct tcataactgt tgccatagcc tactctctta
     8521 gccgaattaa gtttaagttt cgcaatggct ttttaaaact agcccttatt ttaaatatgt
     8581 tccctggttt catgagcatg attgccatct actatatctt aaaggcctta ggtttgacgc
     8641 aaaccctaac agcacttgtc cttgtttatt cctcaggtgc agcccttggt ttttacattg
     8701 caaaaggatt ttttgatacc attccttatt cacttgatga atcagctatg attgatggcg
     8761 ctactcgtat ggatattttc tttaaaataa cccttccttt agcaaaacca atcattgtct
     8821 atactgccct tcttgctttt atggggcctt ggattgactt tatctttgca caagttattt
     8881 taggtgatgc tacaagtaaa tataccgttg ctattggact attttcaatg cttcagccag
     8941 ataccattaa caactggttt atggccttca ctgctggttc tgtcttaatt gccgtcccaa
     9001 tcactctcct cttcatgttt atgcaaaaat actatgttga aggcatcaca ggtggttctg
     9061 ttaaataagt tacaacatac caaaccttct aagggcaagc cctttaggag gtttttgata
     9121 aataaggccg tagataatca tattagtcct cacatttatc ttcagatcag ccaaatgtat
     9181 taatgattcc ttgactttta ccatgaattc ggatataaca aaatgatgac atctaaaatt
     9241 accattaaag atattgcacg agaagccggg gtttctgtgg cgaccgtatc ttacgtgtta
     9301 aaccagcgca atgatcaaaa aatcagcgaa gaaacccgca ataaagtgct acatattagt
     9361 aaccttctta actatacacc taatcaggca gccaggtcat tagtcacaca acaatcaaac
     9421 agtgttgtcc tgtattacaa atatgcagat agcattttag ctagggctga acaactgcat
     9481 accattagct ttctatcccg cctattgcat gagcaggact atcagctggt ttgcctaaat
     9541 gaagatagca ctgaaaaatt tgactgggca gaccttattc tgaccttgga cgttgataat
     9601 gatacctttt acaaaattgg aaagcttaat ttcaaaccct taattgccat tgatagcttt
     9661 gttaatgatc ccctcttctt tcaaattaat acagattatg ctgctctcaa agatcaggct
     9721 aatcagtttt ttaaggatga accttatacc ctgctttcct tgcctatcac taatcgtgag
     9781 cgatatgagc atgtttctgc cttctttcca agtttgcaca ttgtaaacga cctaagagag
     9841 ctaacctact ttgaaaagga aaatctttta ctcattgatg agagtttgga tcaattcttt
     9901 gtgaactctt cgcaaaaact ctgctgccca cgtttttccc ccaaaaaagg gcaagttatc
     9961 ttagagacca tcgaatgggc caaaaaacgc cagcctgata aaaaacataa ccttctggtg
    10021 taacgcctga gaattaacct atcttgacta attccttgta actcaaaacc ttgcctcttt
    10081 agataaagaa gcaaggtttt ttgatgtcta atctaataac agggcctgac ttgtctcatc
    10141 aatctctaaa ctgtgttttg tagaacaccc aggtcagcat taataaggta ccaaatactt
    10201 ggcaattcat aatcagtact ggatcctgaa taaagaaact aagagcaatc gccagtagac
    10261 tcggccaagc caaacaatta accatcaatc caagtccttc agagaagttt cgaatcataa
    10321 atagtcgtga tcgtttagtc agagtcaaaa agaaagcgcc tagaccaatc acaataaggg
    10381 tacccacaca caccatgaga acaaacgtca gcaaaagaaa accaagtaca ctggctttat
    10441 tactgtcata ccattgctga ttgacaaagt ccttcactgc tttaactgtt gtcaggtcag
    10501 cgagtgactg ctgctggcct ctaacgtgag cacggagttc cttgccatca ggggtaacaa
    10561 aacgccattc tttttttgtc acaataattt gtctggttga ttctgatgcc aattgctcta
    10621 aatcgacctt tgtcggtaaa acagccaaaa ccaattcacc atctcgtcga atcagagatt
    10681 gaccctgaaa cgtatctttc tcaatctttc cttgggtcaa tgcttgatag gttttgtctg
    10741 tgataggcga caaactctta gttacaatcc gttccaaggg atacgttgtc atgttagcat
    10801 agtgtaacgt aactggaatc attattaagg cgttcaaaaa aacaataata agaagatttt
    10861 gccaccaagt aaactgagct cggctggcaa acattgccct aggagaaagg caatgtgaaa
    10921 tgtaagaaat aggaaattgt tttttatctg gcattaaaca ttactccttg tcaggttacc
    10981 ctttggtacc accacttgtt aaaccggaaa cgaaattttt ctgtaggaag aaaaagagaa
    11041 ctgaaatagg cactgcaatc agaatagctc ccgcagcaaa taaggttacc ttgggatttc
    11101 tagcatcatt cgtaataaac gattgaagac caactgctac cgtataattt tcttgcgccc
    11161 ttaaaaggaa tttagccaac atgaagtctc cgaaaggacc cataaaagcc cataaggatt
    11221 gtactgcaat cattggtcgc acaagaggaa ggacaatttg gtagaaggtc ctaaagtgtc
    11281 cagaaccatc taacttagca gattcgtcca agtcataagg taccgtgtca aaataaccct
    11341 tcatcaacca ggcattcata ggaataccac caccgacgta aatcaaaatc aagaaccagt
    11401 attggtttaa ggcattaaag agccacgcca taacaaagta agccgttaaa gccgccatgg
    11461 taggaaccat ctgtacgact aggaaaaaga ttaaactttt tttgcgtcca aagaaattgt
    11521 aacggctata cgcataacct gtcaaagtga tgactgttac ttgtatgagc atggtgaaga
    11581 atgccacgat caaggtattc caataccatc ttaagtataa ggtatcttga aagagtgttt
    11641 taaagttaga caaggtccat ggcccatcaa aatgaaaact aaaggctgtg gtatttcctg
    11701 gtctaaaagc tgaactgacc gtcactagta ttgggaataa aatcacgatc actaaggaga
    11761 ttagatagag ataagtcaat aattgggtga agaaacgttt gcgtttcatg gatattgaaa
    11821 cataggtctt tctcatgatc tctcctccat atcaaaagct ttaaatttct taaaggtaat
    11881 caacgatacc gaaatcacta ttacagagat gattaaggtc actgccgacg ccatcgaaaa
    11941 ctgaggcgag gtttgtgtgg tcaacttgta aatccaagaa atcagaatat cagttgtccc
    12001 agctccgccc cccacagtac cagggccccc attattaaac agataaataa tagagaaatt
    12061 attgaagtta aaggtgtact gagagataaa ggttggtgct gcaaccgcaa aaatcattgg
    12121 catggtgata tggcggaatt tctgtaagac tgtcgcacca tcaacggttg ctgcttcgta
    12181 taaatcagct ggaattgcct gtaaaatacc tgataccaaa atatagatat agggaaatcc
    12241 tagccaacct tgcaccataa taacagccac ttttgtccag aaggggtctg tcttccagga
    12301 cacaattccg aagtcaacga aaggaaggaa tttctcgatg aaaggaagaa cctgaacatt
    12361 catggcccca atggaatcat taaagaaatt accaaaactc ataatagaga taaatgctgg
    12421 aacagcccat ggtaacaaga atatcacacc aaagagacgt tttcctctga taaaggattg
    12481 attagcaata acagccgttg caattccaat aacaatttga agacttgtcg ctgctaatgt
    12541 ccagatcagc gtccatgaca aaacagatgt aaatgctgtt ttgaaggtgc ttaattctaa
    12601 gatattaaga aaattcttca accctaccca atccaataaa cgatacggag gaatatgcct
    12661 aaaatcgtaa ttggtaaaag caatcagcaa ggtcactaaa actggaaaaa taatagcgaa
    12721 agccattgct aaataagccg gaatgatgag caagtaagga aatccttttt cataaatcgc
    12781 ttcaagggtc tctttagccg tcaccggaac cttcttgcct tcattcattt gatgtgcaac
    12841 taatttagca tctctcatag caaccaaatg aaaaatacca aataagacta gagtaattaa
    12901 ttgcatggcc cccttaatta acataaataa ggagtgatct tgcattggaa cacttccaag
    12961 actgtaaaga cctgttaaag caggaattcc tagaactaca atttctagta gctctaggaa
    13021 gaaaacaact aataagactc cccctttaac gatttgttta ttgtatagtt gtcccaaacc
    13081 tgggataaca gatagccaca tggctttatt aggcttctgt tgtctttggt tctctaccat
    13141 tttttattcc tcttctcata aatgatttaa ggttatcctc cttatcatac taacttcctt
    13201 ttaagctgac taatttattt ttttatagta atggtttgtg cttcatgacc tgtctttata
    13261 ctataagtct caggacttgt ccataaaaca ttcccaatac tatctttttt aacaagtttg
    13321 actgctattt ctttattgat tggtagatga gtatcaaaga accagtttgg gtacttggca
    13381 atggtctgag tgttattaaa tagaggacca acagcattct tagcgtcatt tgcccccatc
    13441 tcaaaaacat cacccatgag atatagttgt tcccctggta ctgtcttaaa atcattgatg
    13501 agtaaacgaa caggaatttg tttatcagtc aatacttcaa aggcttggta actattgctg
    13561 gtttgcttat cggcagttgt cactgaaatg ttataataat ttgctggaac cgtcggtact
    13621 tttaaggtga taagggtatc tgaccaagaa aggatctcag cagagatttc tccaaaagtc
    13681 acttgacctt tagaattacc aaaaccttga cctgaaatag taatcttatt tccagcaata
    13741 cccactgaag catcgacatc tccaagttgt ggtgtcttgt cctctccttc ataagtccat
    13801 acagcgactt gccccgctcc aagttcaaag ctactaatag tgccatctgc tccaactatt
    13861 aattcttgac cgccaagaag accttctaat ttgtccttgt agctattttg gggcaaggct
    13921 gttttagcat tagtaattgt ataaccattc gtttgatctc tattaatagc cactagtgcg
    13981 acatgattac caaatttacg ctcaaatacc aaaacatgat cactaatcca acgttgttca
    14041 gttgttccat aagctaaagc ttgattttgt tttcttaaag gagctagctt actaatcact
    14101 ttataggctt gtgactcttt attaaaactt ggcatatcac cacgattatt aggatcttta
    14161 tcacctgttg catactgctc tgtaccataa taaatattag gcacacctct agatgttaaa
    14221 agcaaagcat aggcttgatt aacagcagtt tgattattag ccacttttgt tgcgaagcgt
    14281 tccatatcat gattatcaat aaaggtcacc tggtctgtca cttcattagt cacctgatct
    14341 ctattttcaa gaaccttgta aaaatctcgc atggtaaacg ttgaaaaacc tgtatacaac
    14401 tgtctaatag cattggcaaa tctaaaatct aaaagcccca tcccactatt gttagcaaat
    14461 gtcgtcatat cataatcatc gtcggtatgt cccgagaacc attccccgaa aacaaagaca
    14521 ttatgttttt catagatatg actcaaccaa tttttttgcc aaccttgtga catatgttta
    14581 acagcgtcaa ctcggatacc atcaacaccc aggtctaacc atttatcaat agcttctttc
    14641 atgtactggt caactttcgg attaatgtta tttaaatcag ctagtccgta cattgaatga
    14701 taaatagaat tttcataagt actaaaatcg gtccaagatt catgattaaa aatcttgtct
    14761 ttatcatctg aaaatttacc aactaattta ccgtttttat ataaagcgcc atcttctttg
    14821 aaagttgtgc cttctttttc ggctgtagac gtatgattag gagcaaaatc aataactacc
    14881 ttaatatggt gttgatgagc tactttgact agttgttgaa agtctgcttc agtgccaaaa
    14941 tgctggtttg ttttaaagaa gtccttagcc caatacccat gatatgcagc acttccatta
    15001 gaaggatcaa tactgtcgat attttcaaca ggagaagaaa tccaaatggc agaaatcccc
    15061 atatctgtca ggtaaccatc cttaatcttg gcgatgattc cttgccaatc acctccatgg
    15121 tattttttaa ggtcattttt atcaaaaaca tcagtcttac cattattaga ggtattacca
    15181 tcgttaaaac gatctgttac aatctgataa atcgtatctg ttgaaaaatc tgctttgttg
    15241 gtgacagaag cattatcggc agcagaaaca gttagtggaa atgaaattaa gccaagtaaa
    15301 acagcacttt tcgttaataa cttataagtt ttgatatgta attctctcat cgctttttcc
    15361 ttttaaaata accttttagt tactaaacac aatatatcca tcctggagta attgtttttt
    15421 gtttttagtt accccattgc ttgtcaaaat ctgtcctcct tccttaatag aataagcact
    15481 ctcaccattg ttaagaacaa gtgaaatgct ttgctcacct aagctgcgtt tcagaatgag
    15541 gcagtcttcg tcataatcaa tgctaatagc tccttcttga agagctggct gttggcgaag
    15601 agcaataatc gcttttactt tgctaaagaa atccttgtcc cgctcagcat catcccaagg
    15661 aaaaccgcga cgcgaatcag ggtcgtaacc cccttccatt gccacttctg tcccataata
    15721 caagcaagga atgcccataa agataaagag caatgctaaa gcagctaaca atttatcttt
    15781 ggaaccctta acttgagtga agaaacgatg cgtatcatga gaatccaaaa gattcaagtt
    15841 catgcgattg acttgccaag tgtttcgcat caaaatgttg cttaatttct cagccaattg
    15901 ttttgatgtc atggtttcaa aagcaaagta atccaaacag gctttggtga aagcataatt
    15961 cattatccca tcatactgat cccctgccaa ataaggatag gcatcatgcc agttctcccc
    16021 aattaaaatg gcatctgctt tttccgcttt aaccgcctga cggaaacgtc gccaaaaatc
    16081 atgagatact tcatcagaaa catctaggcg ccaaccatcg atacaaaatt ctttaatcca
    16141 gtatcgccca atctcaatca gatagtcttg cacgtctcta tttgaggtat tccatttggg
    16201 catatagtta catccagcaa aggtttcata gtttactagg tccatggatg ggtgttcatc
    16261 atgagtcata aaccaatcaa aaaacttaga ctctttaccg tatctcaaga catcctgaaa
    16321 ttccacggca tcactgctgg catggttaaa gacagcgtct agaataatct tgattcccat
    16381 tttgtgagct aaatcaatca attcttgcaa atcatattta gttccaaatt gtggatcaat
    16441 agcgtagtaa tcactaatat catatttgtg attactaatg gattgaaaaa ttggcgttaa
    16501 atagatgaca gtaatcccta aatctttgag gtaatctaat ttttctgtaa ttccctttaa
    16561 atctccacca gcaaaacttt taggagtcgg tttttctaac caacccatgt tgacataact
    16621 gtcacctttt tcccaatccc cacggttaaa gcgatcaaca aagatttgat aaaaaaccgc
    16681 gtcttttgtc caaggaatga cttgatgaac atcaacagca ttgatataag gcatttggaa
    16741 gaaattataa aacccatttg aaaaatcgta actagtagtt aacccatctt cactaaaata
    16801 ataggtttga ccttcttctt caattttaaa aatgtaggcc aacctcacat cgtctaatgt
    16861 taaagtagtt tcataataag catgcgtatg gtctgtatag gcaactgaca tttccaattc
    16921 ttcttggaat tcatgataac catatttagg cccgtggacc aaattaacag taatacctct
    16981 atcttctttg gccacgcgta atcggatgac aagttccttt tgagatatgg caaagcaata
    17041 cctcgaatca ggaatatgga gcattccggc aacattcatt tctacctcca ttaataaagt
    17101 attgacgatt acttcttttt ctttaaaacg aaaaaatagc agagcgactc atacttctat
    17161 atttttagta tctgtttcat cttcatcaaa ctaacagaaa tagaagctgg gcgtacttct
    17221 ttatcccagc ctcccttctt tgccttattc agcgtatttt tgttcaatag cttccttaat
    17281 agtttttgct gcttcttttg cagctttaga tgcttcttcc ttaccagaag ccacgttaaa
    17341 gaacatattt gcacctggtt cccaaacttc agccatttct ggaatatttg gcattggttg
    17401 tgcgctctca aactgactaa tcacagcttt cgttaactca ttaccctctt tagaagcata
    17461 gttacgtgct gttaagttag ctggaatttc ttgtgtttta tcatagaaac gtttttggtt
    17521 ttcagcattg gtcacataat caaggaattt ttgagcagtc gtttttcctt tagaatagtt
    17581 tgaaataacc caagctttac cacctgcgaa cggttggtat tttttaccat ttgtcaaagt
    17641 aggaatttcc attaccccaa agttaacacc agcatcttta aaggagctag ctgcccatgg
    17701 accatcaata atgacaccag cttttttaga aataaattgt tcagtgataa aatcaccagc
    17761 ttttttagtg tcttgcattc cttgaggcca agtgccatac cactgtttcg cgtagtttaa
    17821 cccttcaaca gttccgtcgt tacctaggcc taaatcactt ggtttggttc ctttatcacc
    17881 aaagatataa ccgccataac cggcaattaa accataacca taatagaagt ctgtccattt
    17941 cgccaagaaa cctacagatt ttcctggttc agaggcaaaa gcaaacttag aatctttttg
    18001 taagacttct aattcagtaa agcttttagg tgcttgaggc actaaatcct tattataata
    18061 agttaccaaa gtttcaatta cgtctggtgc accataagtt ttaccatcaa tggttactaa
    18121 tttcttaacc gtatcatcaa actctttgct gtttcctaaa gtaacctcag cgatttgacc
    18181 ttcagttcct aagccaccta cacggtcaaa aggtgctaaa aagacatctg gtgaagctcc
    18241 tgttggacca tccgttgata gcttatcaag agtatccatc ataccttctt ttttgacttt
    18301 aacggtgact ttatgttctt tttcaaactc acccttaata ctcttaatgt aatcaacata
    18361 tccctcatca acagagacta caatagtctt tccgtctgtt tccttaacgc ctttaccacc
    18421 accacttacg caagcagtta ggggtagcac tgaagctaag gataaagttc caagcacaga
    18481 tgtttttttc caattccatg acataacatg ttcctcccga aatggttttt tcttaaacac
    18541 aaatcaaatc atctatctac tctcatcata ttagcaaata aaagataaca taaggcattt
    18601 aaaattaata acagtaatag tttcgaaact taaacgttta agttaagttt atcgtaatcg
    18661 ctttcatttg tcaataactt ttttactttt cttttctttt aagatcatat cagtagtttt
    18721 cctgctaatt gaaaacaagg catttactgt cacacaagtc tgtcctttct ttttttctac
    18781 aactttattt cgtcagcgta aataaaacaa aagctctata atctcttgaa tgggtaaatc
    18841 cactgtagag attatagagc tttttgaata tcaaaaaagt cccataagat ttataatgaa
    18901 aagcaaccaa actctcatta gaaagactct tatggaacat atcaatcata ccttacaaca
    18961 catcggaatt aaagacaaaa atatcactct aaacaatgtt ttgaaggatg aatctcctac
    19021 tgagctcgtt actacttatc agaaaggtag ataggaaatc ctgatgaaga gtgtgtccaa
    19081 gggattatca attaggtctt agattacaaa atagtaattc aggtatgctc tactgttttc
    19141 aacactagtt ctgcaatttg ttttcgagct tgcttagctt ctcttattgg aaaaataaca
    19201 tacgcatgaa tcattttttc tgcaataatc agctgattag caattcgttg tcttgttaat
    19261 tgatcatgta aagacataat gtctgggtaa aaaatctctt ttgtcccaac aaaggtcgtt
    19321 atttcaggca aatcctcata atcaccatac aaaggactga cataaggaag ttgcatatta
    19381 ccttgaccac ctgcccatag ttcacctata tcacgaagac gacgagcatc taggtaagct
    19441 tcttctgctt caaaatcctt gtaggcttga tttttaaaac taacatctaa ccaaggagat
    19501 ataagaatta attgctttgg ctggggtgct gttttagtat ctcgtatata cttagcaaac
    19561 gccaaagcca tacccccacc agaagaatca ccaatgacac taacttgact cggtccatta
    19621 gacaagcaaa attgctggta agcttcaact aatttaggat agacatcttg ataagtagca
    19681 tacggtactt taggaaagtt aggaaaaaca accttggcat tagtcagctg tataagatgc
    19741 ttaaccgtct taaaatgaag tttttcgggt cgtcgtaaat agacactgcc ataaagataa
    19801 taaataacag gctgatgact ttgcttttga tcattaatga taaaaacttg catcccgttc
    19861 acatcgtatt cttgagttga aaaacctact atctcttcag gattatctag atgataaggg
    19921 atattacgat tacgctgcgt ctcaaccccc ttataattat caactttttt agataactta
    19981 tactttctat aagcaaacat tttctcaacc aaccaacttg aaaaggagcg cttttgacca
    20041 tattcaacta tcaaaatacc tagtatcact ccaaaaaaaa tcaccacagc aataatgacc
    20101 acttattatc ctcgttttct aatattattt tactattgca agtctttcct atttgcccaa
    20161 attttactat aaaaataagg gttcatttta tagttgggca ctggctgctt cgtttctaga
    20221 aatggttgca ctttcttatc aaaagctaac cagccattcc aaccgagatg gatggtatct
    20281 tgcataaagt aggactcacc accatctttt gagaagtcag caatgcgatg aaatccttgt
    20341 gactttaact ggaattttat tttacgaact gccgcttgat acttatcttg atttaagccg
    20401 gtataatccg cccaagcttt attaacagga gttataacaa agagtacatc tgtttttcgt
    20461 ttagcaaatt ctgataataa aagctgaaaa tcattgtatt ctggtgacgc caggtaacta
    20521 taattaactt ggaaattctt ataaagattg tatttaggtg ctattcgttt acgataaaat
    20581 gtatttttaa tcccaaaacg gttgttggtt gttgctaatt ggcctctctt agttgctaat
    20641 gcattcaatt gtttatacga aaatacttta ggtaatccct taacgcgagg caaaattctt
    20701 ttttcatagt tagtagattt gcctaaaaaa ctaaaaaggg actcttctct caatgctact
    20761 tgatgttgac atttcaaaat agctctgtct aaccgactaa gagacttacc cttacttact
    20821 tttttcaata agtttgattt agacacacca gggttaagct caagcaaacg ctttgctgca
    20881 aactgtgatt ctttatcagt tctagctttt agtaaaaatt caatcacttg agtgttagac
    20941 aagtacatct gaaccgcact aggattaatc ccttgagcag taaaccattg aggagatact
    21001 acaaagatgg cttttttctt ttgcatttca ttggtaattt gttgcatacc ataataatgc
    21061 gacaaagatg ctgatcctct cttaccaatt aaaaatggtc tatagctccg cttgtagcgc
    21121 tctgcaagca ccgaagggtg catactatcc attcgactcc attcgctaga accaaaaaaa
    21181 ggcacaaaac gacaagtctc atctgataaa gcttgtcttt taatcaaacc atttttaaaa
    21241 gaactatctg tgatcgcaac ggcatttgct ttttcctgag ctatggaatg atcaagttgt
    21301 gtaggaaaac taaaaatagt aatcactact aaaacaaagg caataagaag aggacctaga
    21361 attaaccaga gtctcttaag catgttgcaa ttcctctaca ccttgaacaa tcttagttac
    21421 tgtgttccaa tcatcacgac caaattctga aataggtact ttaatattga acgttgactc
    21481 taactcaaca atcaattcga ctgtcccaag actatccaac acacctgcat caaacaagtc
    21541 ttcgtccatc atctctgaaa catcttccat aaacaaacgg tcaaacaatt caattacagt
    21601 ttcttcaata gacattttta tacttccttt tattaatgta gtggtctgtt aaaccaaagg
    21661 tcatttaaaa aaccagagaa taacagaagt gaaaacatga caacgtggaa agttaccacg
    21721 atacataaga catggaatgc cctgctttga aagaggggtg ataagccttt tttcttacgg
    21781 tgacggttga tttctttttt cttacgaatc caagcatcat taataataag tcctatacca
    21841 tggaaaagcc cataagcgat ataataccag gttaagccat gccaaaatcc catgactagc
    21901 atattaacca gataggcaac acctgaagtg acatttcggt ttttaaaggt tttatgctta
    21961 atcaatagat gaaccaatcg cataaacaca taatctctaa accagaagga caaactcatg
    22021 tgccatctat tccaaaaatc tttcaaactt gcagataaga aaggcatgtt aaagttttct
    22081 ggtgttcgaa tacccaatag gtaggaaata ccaatcgcaa acatcgaata accagcaaag
    22141 tcaaaaaaga gattcaagcc atatacgtac ataaccagaa tagtttcctt attaaagtag
    22201 ccaccaacca tcaaagcctt attttcaagg agaggaagta agatgccacc caaacaatag
    22261 gaaatgatat gcttgtatag aaaacctaac atgagataca tgactgcctt attgagcatt
    22321 gcaaaataat catcacgttc aggaagttta tgataatctt cctgaaaatg tctgaagcga
    22381 tcaatggggc cacttgagaa ggtggggaag aaaatcatga agcgaataaa atccggtaaa
    22441 cgaacctctt ggagagtccc gtcacgcatc tcaataatca ttccaatact tttaaaggtc
    22501 aaatatgaaa tccctagaaa actaaagagt gtttgagaag aaggacctgt taggggagca
    22561 actttgaccc agataagggg gaataaggcc atcgcaatag ctaagtaaaa cactcctgca
    22621 ctattagcat tctcacggta tttctggtaa gcaaaaacac tgatgatttg ccacaaaaga
    22681 taaaagagaa aagcaactaa ttgttgataa tgatctcctc caaacataaa gagaataaat
    22741 accaaactca ccaaggtttc ataagtgctt aagcgctttt gtttaaaaat cccaatgatg
    22801 actggtaaga aagccaaaat aagatagaca aagtaaatgg ggttaccgta aggttccatg
    22861 tagggaatgt ggctaaaaaa catcatcatc ggttattgac ctcattaatc aaagttttaa
    22921 tatctatctt accatttgga gttaatggta gactatctcg ataaagaaac ttagaaggca
    22981 tcatataact catcatatga tccttaacag aggctttgat agctttggtt aactccaatt
    23041 ccctatcaaa acgctcttta accccatctt tgacaacgat ataggccaag agattttgaa
    23101 ccttatgttc tttattgtac cttggaacag ccacagccga tgctaccatt ggtgattgat
    23161 tcaactgttg ggaaacatct tctagttcaa tacgataacc agcatattta atctgaaaat
    23221 ccaaacgccc accatacaac aaaatattat cttctgtcag tgagccaatg tcccctgtat
    23281 gataagcagg ctgtccttta aaagtaaaga aagcttctgc ggttttctca ggattgttga
    23341 ggtaaccttt tgacacagca ggccctgtaa caataatttc accctgctcc cctgaagata
    23401 actccttacc atcttcatca ataatatagg tcggagaatc tggtttgggg tagccaatcg
    23461 gcaagcgggt ataattatcc accatttctc tggtaatttc aatagcggat aaggctacag
    23521 tcgcttctgt tggtccatag gcattgataa tcttggcact cgggaaacgt tcaaagagtt
    23581 ttcgagccgt agaaacggtt aattcttcgc catcaaaata aaaatgcgtt aaagctggca
    23641 tcttagcctg gcaaaaatca tcgctaagca tggccatatc agcaaatgaa ggagtcgatg
    23701 tccaaatgcc aactggaagt tgcgcaatcg ttgtaaataa ctgtttaaag tctgccacca
    23761 attcttttgg aagtgcaaat aaggttccac ctaaagccaa ggtaggtgcc cagtacatga
    23821 cagaaaggtc aaaggaatag ggtggctgcg ccagcatttg tggctgttta ggtacatcaa
    23881 atgctgcatc ttcgatcatc cagttggtaa agctcaaaag attgtcatgt gaaatttgca
    23941 ccccttttgg ttggcccgtt gttccagagg tgaaaataat gtaataatta tcatctcctt
    24001 taacagaatg tgttcgctca tagggcattt ctgctaattt tgctgactca atctctgata
    24061 gtgagacaag ggaaatccct tcaatagtaa gagggaattc ctcaatagca atgattaaac
    24121 tgggctttgc aatctcaata atcgctaaaa tacgttctgg tgctgaatgc acatctactg
    24181 ggatataggc atgaccagat ttggttaaag ctacaaaagt agctaacata tcataagttt
    24241 gcgccccaaa aaccaaaact ggagattttg ccagtaaagc taagctatct ataaatgcag
    24301 caatgctatc agaatctctt ttgagttgtc cgtaagtgcg gcgttcccct aaacaatcat
    24361 aaactggaaa atcagcctgt gtctgagcaa actgctcaat actgtcaatc atatccttaa
    24421 tcatcagtca atctcctaaa attcattata aataaagtta ctttgaccat gtcctagata
    24481 accaaagaaa tagaccagca acattaaaat cagataaaat aaaattgtct tgccaataaa
    24541 aacagcaatt cctctcattc ttgatttgtt ttttatcata ctttaccttc ttgtattagt
    24601 gaaataatgt cctaaaaaat tctactcgaa aaaacctaac tt
//
LOCUS       DAXWIQ010000026        24301 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000026 DAXWIQ010000000
VERSION     DAXWIQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24301
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.026"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..180
                     /locus_tag="ACGEUI_001070"
     CDS             <1..180
                     /locus_tag="ACGEUI_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFF8165911.1"
                     /translation="DSLTRQVKNIYFLQNTKDDMLSFSDINVMPRTQDIEREFNVKEW
                     IGDAIVHVESKLNAR"
     gene            183..707
                     /locus_tag="ACGEUI_001071"
     CDS             183..707
                     /locus_tag="ACGEUI_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFF8165912.1"
                     /translation="MNIEEAKRAIRELEAFNYFDFKGNLIKRIDVLGIIDQLGQPKVV
                     VPKPVFEMIKFRKEHDQDLTTTFVNIHAYPDNVFHWVANNGDLFAQAWLAYPNITIEK
                     EKLYTVEIPNPNREGRGHAKFVLERQGNKVFLVKRKSKDCYYNKNSNHLTESEIRKDF
                     DWAWREGFAEEVTE"
     gene            704..970
                     /locus_tag="ACGEUI_001072"
     CDS             704..970
                     /locus_tag="ACGEUI_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165913.1"
                     /translation="MKEKTIFISKKYANDFNNDKYNLSSGYYFKNGEQHDIAIVKYGE
                     QDYLKNTDLAYVVCDKIVDGESIGFVYHGEYETWRFKLLNTEAN"
     gene            1256..1690
                     /locus_tag="ACGEUI_001073"
     CDS             1256..1690
                     /locus_tag="ACGEUI_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFF8165914.1"
                     /translation="MTFFPEIDIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQPYGTPSKPVERLALNRVSAEQELDAIEQAVSMILEPERRRILYDKYLAPYKKAD
                     KVIYTELCMSESFYYDTLDIALLAFAELYREGVLLAEKGVFS"
     gene            2300..2680
                     /locus_tag="ACGEUI_001074"
     CDS             2300..2680
                     /locus_tag="ACGEUI_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165915.1"
                     /translation="MARANKAKMLDAIEFKLLNPTANQAQVAEIIGITDRQLRNWEND
                     PEDKSGWNKLNDEIAEKAFGKYALSAIGTLVNLSKNAKSELVRLQASQDILDRAGYKP
                     TDKQEISIDEPIVLANSWVQDNGS"
     gene            2670..3944
                     /locus_tag="ACGEUI_001075"
     CDS             2670..3944
                     /locus_tag="ACGEUI_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFF8165916.1"
                     /translation="MVVDLADIIPIGFKPVVQATWNPQILNIACKGGRGSGKSSNIAF
                     IISRLIIQYPVNAVCIRKTDNTLEQSVYEQIKWAISEQGLERYFKFNKSPLRITYIPR
                     GNYIVFRGAQNPERIKSLKDSRFPFAIGWIEELAEFKTEDEVKTITNSLLRGELGDGL
                     FYKFFYTYNPPKRKQSWVNKKYESQFQPSNTFVHASTYKDNPFIAKEFIAEAEATRER
                     SERRYRWEYLGEAIGSGVVPFDNLRFERITDEQVADFDNIRNGIDYGYATDPLAFVRW
                     HYDKKKNGIYAIDEYYGQKISNRQLAKWLTTKGYQSDEMFAESAEPKSNAELKNEFGI
                     KRIKGVKKGPDSVEFGERWLDDLDFICIDPKRTPNIAREFENIDYQVDRDGNPKPRLE
                     DKDNHAIDATRYAMSDDMRATKTIVKTFKGGI"
     gene            3944..5269
                     /locus_tag="ACGEUI_001076"
     CDS             3944..5269
                     /locus_tag="ACGEUI_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFF8165917.1"
                     /translation="MPELFIVPSDTEMTKDLLSELIQKHRSFNLSYSAYKQLYEGDHA
                     ILQQKQKEQYKPDNRLVVNFAKYIVDTFNGYFIGVPVQTSHENKQVSNYLELLDGYND
                     QDDNNAELSKICSIYGHGYELVFNDENAEVGITYLTPLEGFIVYDDSIRQKPLFAVRY
                     FYNKDGVLEGSYSDASKITYFKDGEKDLEIGESDPHPFDGVPMIEYVENEERQSLLAS
                     VVTLINAFNKAISEKANDVEYFADAYLKILGAELDDETLKSLRDTRIINLKDTDAQQL
                     TVEFLQKPDADATQEHLLDRLENLIFRTAMVANISDESFGTASGIALRYRLQAMDNLA
                     KTKERKFMSGMNRRYKLIASYPTSKIGPKDWIGIKYKFTRNLPANLLEESQIAGNLAG
                     IVSEETQVGVLSIVENPQKEIEKKNSDRPTLISRKAGGLNGQDTTTVLE"
     gene            5238..6146
                     /locus_tag="ACGEUI_001077"
     CDS             5238..6146
                     /locus_tag="ACGEUI_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFF8165918.1"
                     /translation="MAKTPQQYWNERQKQLWANLETSERALQTKLSKYYADEAKSLEK
                     EIGAYFSKYGKDNVIEYRNLLQQLSKADKDLLYRDCERFAKKYPQRADLMPVRTSIYK
                     LDRLQGLELSIKIQQLEIGAIEEAELTKHLTTAFKKGYQETAKTIGFQADKVSAELFV
                     NNDWTGKGDFSSSIWSNKDKLVDYLTNDFKTAIIRGDSFDKVVKQMSERFTVRSQSDI
                     TRLIMTEGTYVNNQAMMAPFEDSDDFEEYEFVSVLDSSTSSICRGLDGHKFKLKDRQV
                     GVNFPPMHANCRSTFAMVIPDDYLER"
     gene            6153..6422
                     /locus_tag="ACGEUI_001078"
     CDS             6153..6422
                     /locus_tag="ACGEUI_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165919.1"
                     /translation="MNKRIKKKRKLETAIVMIVAENAMQAEALRNQNKEIMELKSIVQ
                     RNALATNEELATVKAATLDNQSAIKSIGDDVDYIKQNYKRKWRKK"
     gene            6424..6558
                     /locus_tag="ACGEUI_001079"
     CDS             6424..6558
                     /locus_tag="ACGEUI_001079"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165920.1"
                     /translation="MVLEQAELEMVRNQRLVMINPEMVTITKTEYDLLIEIKNKFLES
                     "
     gene            6667..7236
                     /locus_tag="ACGEUI_001080"
     CDS             6667..7236
                     /locus_tag="ACGEUI_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFF8165921.1"
                     /translation="MENEEILEQSGAQEEAKEQTFDDILSDPKKQAEFDKRVAKAIDT
                     ARNKWVAETEEKENEAKRLAKMNAEQKAQHEKAKLEARIAELEAERTLSEMKSAARTM
                     LSEANINISDALLSQLVSTDADKTKNAVEAFSEAFSEAIEKEVKERLKSPTPKKSNGN
                     TGLTKEQILAVKDTAERQKLIAENIGLFQ"
     gene            7255..8145
                     /locus_tag="ACGEUI_001081"
     CDS             7255..8145
                     /locus_tag="ACGEUI_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFF8165922.1"
                     /translation="MVTSRTYPEENLIKSTDLKYPITIDVTNKFQENISKLLEMLGVT
                     RKISVSEGMTLKTYAGYDVTLAEGNVPEGEVIPLSKVERKIHSEKKIELKKYRKATTG
                     EDIQMYGSNEAVTNTDNALVRQLQKKIRTDFVTALKTGTGTQDALGAGLQGALASAWG
                     KLQVLFEDYGSERAIVFANSLDVAEYIAKAGITTQTAFGLTYLVDFTGTVIISTNDVT
                     KGEIWATVPENIIFAYINPNNSELAKEFNLYGDPTGYIGMNHFQENTTLTIQTLLVSG
                     MLMYPERIDGIVKVTLTPGV"
     gene            8158..8451
                     /locus_tag="ACGEUI_001082"
     CDS             8158..8451
                     /locus_tag="ACGEUI_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HeH/LEM domain-containing protein"
                     /protein_id="HFF8165923.1"
                     /translation="MAYRVKARFFDLLDNSFLYEVGDSFPRKGYEPSKERLESLLSSN
                     NTEHKPFIEVSGNGNSLDGLKVDELKAKAAELGIELPSNAKKAEIIELLQAHI"
     gene            8465..8809
                     /locus_tag="ACGEUI_001083"
     CDS             8465..8809
                     /locus_tag="ACGEUI_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165924.1"
                     /translation="MDETKQAIIDRVRVRLADETSLKEELLEELTQTAIDRINLKVGD
                     VVFNPLFNSIAVDVVVKMYRRMYFEGIDTEKADTISTKFIENVLAEYGEELASYKKDR
                     LAILNKKVVRFL"
     gene            8806..9117
                     /locus_tag="ACGEUI_001084"
     CDS             8806..9117
                     /locus_tag="ACGEUI_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165925.1"
                     /translation="MMFVNFDLVTSQKTGEKDRLGNDITKNVVKRVAKGRFTEWSADD
                     VSLYGRDLTSSARKLLTNQVSKAEAKQASHVVIDGSKYKVESVKDLGRWRLLVIKGYR
                     L"
     gene            9114..9509
                     /locus_tag="ACGEUI_001085"
     CDS             9114..9509
                     /locus_tag="ACGEUI_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165926.1"
                     /translation="MRMSMTGMPALEVKLRSMSEKRWDRVANKNLTEMFNRAARPPGT
                     PIGKNTKRHKSGELLRSRRLKKVNSSKDVITGNFGYIKDYAPHVEYGHRIVRNGKQVG
                     YANGTKYLFNNVKKQREIYRQDMLNELRR"
     gene            9511..9921
                     /locus_tag="ACGEUI_001086"
     CDS             9511..9921
                     /locus_tag="ACGEUI_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5072 family protein"
                     /protein_id="HFF8165927.1"
                     /translation="MLKKLGLVDLHASIKQKIEDKTGLMAYDHVPEDMPSPFYFIEVV
                     DKRPEDTKVMWCEVFTVWIHAIAEAGKSKIAIYDMIEKLEEALTEELVLPEEIDILRQ
                     SEVGMQSLQEDETGEMHAIVAYEIKVSYGFKVKV"
     gene            9933..10439
                     /locus_tag="ACGEUI_001087"
     CDS             9933..10439
                     /locus_tag="ACGEUI_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HFF8165928.1"
                     /translation="MVAFDQNVYCNFDTSATKAIAGKDIILAIFDKTGANLLAISGQQ
                     GLTINRTADSIEVSSKDTKGGWKSKIPGMKEWSIDNDGLYVPSDETHKQLGEAFEKSE
                     FVCIKVINGKTKKGMFGGLASVSDYSLEAPYDDGMTYSISLEGNGALIDLSTLSSEDA
                     EKVTAMPS"
     gene            10452..10769
                     /locus_tag="ACGEUI_001088"
     CDS             10452..10769
                     /locus_tag="ACGEUI_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165929.1"
                     /translation="MDKELIIIDEKQYELKYNEKTIETVEALTGKAFMDVIVNNKGML
                     SLSMLRQYFANALYAVEGGRVSSEQGSNIFTKVLNTKGYAYVNMLVINTIQRDCPFFF
                     LGA"
     gene            10742..11200
                     /locus_tag="ACGEUI_001089"
     CDS             10742..11200
                     /locus_tag="ACGEUI_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165930.1"
                     /translation="MPFFLPRRLVDFEYLGGSGDSTDVEYDRLASQYHKDIDFAFYFV
                     NFGTTKSEFLELTRREKAFIRKAWEDKQVRESELMRNAVLNAVSNAMRKKSAKFVDLW
                     KRQQQPANMEIVEAHLEIINKNIADEGKYWVDLVYQANNMTKPSEGAENG"
     gene            11193..12998
                     /locus_tag="ACGEUI_001090"
     CDS             11193..12998
                     /locus_tag="ACGEUI_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8165931.1"
                     /translation="MADYTLGVQITGDASKMQKAFEKAQEQAEKLKEKIKDSNNESGN
                     SFDGLTTKIDAISGKLSAYGDKISSIGKNMTTMFTLPAVAGITAVVKEYANLEQSIGG
                     VETLFKKSAKAVQKNANTAFRRAGVSANEYMEQVTSFSASLISSLGGDTAKAVGVADT
                     AMVDMSDNANKFGTNIGLIQNAYQGFAKGNYTMLDNLRLGFGGTKEEMQRLLDEAGKL
                     SGMEFDINNFSDIIQAIHIIQDNLGVTGTTAREASETISGSFSQAKASVIDFMGSLGG
                     DGSDVDAKMQAMIQSITVFADNVKEALGRIWDNLPLQEYQKNLLLIAVVGGPILVVVG
                     TIFGAIANIMTVVSGILTVLGMMTPIGWIIAAAIAAIVAAVVFWDDIVAEVGQKFELV
                     KIIGMAMWESFKDGVSSFGKSIQNVVGKIGGWFAEAWNAAADTATRIFTKVGDTISGI
                     FSGVVSGIKSLINGAIGGINKVIGITNKLPGVNIPKIPYLARGTSDWQGGFARINEGG
                     RGELVHMPNGATVVPHDVSMKYARESAKANSGVAYMDSNDEIAKSALKLANDAVKRPV
                     VLNINGREVAKTTGGDMRDYLNSRDMTLKRLRGEV"
     gene            12999..14483
                     /locus_tag="ACGEUI_001091"
     CDS             12999..14483
                     /locus_tag="ACGEUI_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="distal tail protein Dit"
                     /protein_id="HFF8165932.1"
                     /translation="MATMTFNDVDLSSLITIMSINRDIGNERQADTNDAPFIGLHIKK
                     IKTSAKIITVDFYLKDRTNEYALNQLKHKLAGIFNVNEQVKVTFSDEPDKYYLAIPIN
                     KISASDPIAWLSLVSLELLVPDGVAHSVSYKKITNYRQDGKKLLIDITNNGNVDAHPI
                     ITVKHNAENGYLAFVNKSSVFEVGDREIADAEIREKSVVAYDFKDERIVNALTAGKKN
                     VAILNDSTQVLDKNLLLNTVWGRKHLELEGSVAPSGNHAGTLTFDIPDGGSLYDYLWW
                     RQIFWVGTVSQLGFIKIMVSDENGHFLYGVETIKRTLGTKTEYNFLVTNGKGGYKITD
                     LRWTFDATHFETQNPFNEPRGWSDMTRADDTVSVFWWGSQNKRVFPELKGKKSKQVHI
                     AIGTIQGSPLVTHMYIDGFYYRKDKVPYEFNIPNAFGAGTEVVINAENDTVLVDNIPK
                     ANIIADGFFSFPKIPPGTSTLEVYSSSWSTANPDITLNFEERWL"
     gene            14484..17924
                     /locus_tag="ACGEUI_001092"
     CDS             14484..17924
                     /locus_tag="ACGEUI_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8165933.1"
                     /translation="MLITIHNANLEKVAYIDNDKQDTLNYYDDKFSQYLNTANSTFEF
                     TVYKRGIKSDTVKEKAYLTLTERSFVSFKYNGKSYLFNVMTTDETDIEIRCYCENLNL
                     ELLNEYAGPYKAATQMSFVDYCNLFGILKNGAITIGTNETTDQKRTIEWTGQDTNLKR
                     LLSIANNFDAEIEFVTHLKNDSSLKSFVMNVYKKNDATNQGVGRRRDDIILQYGKNIE
                     SVRRKINKTGIYNAIRPSGKITTTTTTTTAKQGSVQTGSVLWSGGNLTYAGHVMQSSV
                     VNTILSLCSKYKLLPSGVFSQLYLESFWGDTPVGRADNNWGGITWTGATTRPSGINVS
                     QGQSRAEGGYYNHYASVDDYLKDYAYLLAEQGIYAVKGKLTIDEYTRGLFRVGGATYD
                     YAAAGYDHYAPLMRDIRAGINRNNNGAMDNVDNQFKNGGSTSQNTTQIAAKTKAVLAE
                     ANGLKGQRVGSGQCYALAAWYAMKLDGPGLNGGVTSFRGLIGAGAAAAQIGTDYNWGQ
                     FGWKVVQPNKVADLITGSIVNIRANAGSPVFTGAWGHTVVVKSLSGDTLTVLEQNYNN
                     VQTVQEHTYSASAYLSVVQTVCYPPEIVQGKRVEGTAQAEQPQSETTTISEEKEVLIN
                     PSLYREWKNEAGQVEFYVKNSMLYAPLSKSLYPSAFTGIETDDNWIRKDLDVDTESEE
                     KLISVALADLRKHCYPAVTYEVSGFIGDLDIGDTIKINDPEYTPSLILEARVSEQHIS
                     FTEPNQNKTVFDNYRALESKVSQGLIDRMNELAEAAKPYDLRLMTDNGNVFLNGEGRT
                     ILTAELWKGNKKFDASYQFKRDGQLVGAGLQLAVDAKDVPADRPLIITVEAYLNNELI
                     ASKQITFTNSLGEQGPAGRGIVSTEDYYLASPNRTGVTSATSGWTKTPQEITETNKYH
                     WYYHVDVYSDGTRKETTPAIIGVYGDKGSDGKQGKRGEMGPSGPPGALDKKQLQDINN
                     KIDGKADQNLTIEQINKLAELQSIANAELQAKASIDALASLQKQVQSAIAAMNASQKL
                     SEQDLITASQRAIKATNDILDLKEQWNFIDNYMSASEEGLIIGSKDGTSSVRVAKDRI
                     AFYSAGAEVASITGGMLKIDNGMFVATLQVGHFREEMYKVDGVDKHINVTRYYETIVG
                     "
     gene            17929..19791
                     /locus_tag="ACGEUI_001093"
     CDS             17929..19791
                     /locus_tag="ACGEUI_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF859 family phage minor structural protein"
                     /protein_id="HFF8165934.1"
                     /translation="MAEFWSNNDRGYRIKLTIDQVSQNTLANSSQVRVKLDLLNTTTT
                     FTQYSCSAYVDLNGQRIDWSDSPSVLRYNSTISLIDRTITVNHNADGTKSFGFIASFS
                     GSGGWSPGTLTVGSGTFTLTTIPRSSSVVVSSGTIGSAITININRQSSSFKHNLRYQW
                     GNKTGTIASDVDTSAVWTIPLDFASDIPNSTSGSGTIYVDTYNDSTLTGTQQVPFTVT
                     VPDSMKPTLSSISLSDAHTVAGNVVSSADYFIQVYSDIRVNFESASGSYGSTIKGYYA
                     EIVDKGQSTDQNGGTLGNMLYDGQITIRAKVIDSRGRESQPMDKTVTVLKYFPPALSF
                     DVARSGYGSDTLTVTRRASIAPLSVFGTQKNTMTLSFSVAELGSSYFSANNGSASGAW
                     ANVSSLVNSSANLYGAFSPTKSYTVKGILSDKFSRTEFTFDVGTESVVMSIAKNGIGF
                     QKIWEKGAIDAKGDAYISGKLFVNNTEVKPSFDKTEILNMVYPVGAIYMSTSSANPST
                     FIGGTWQRYAQGRTIVGVSENETEFNYVGKTGGAKTHTLTNEEMPSHSHGDKTISSGG
                     RPISSNAGWDNTNVGLYKSTDYNQINAFNKSSGGDQPHNNLQPYITTYIWLRTA"
     gene            19802..20149
                     /locus_tag="ACGEUI_001094"
     CDS             19802..20149
                     /locus_tag="ACGEUI_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HFF8165935.1"
                     /translation="MLVITSNYPEQMPDGSVSGAKVMLDGIDDHAGWHIPLTLLKEYL
                     DKPQSEVMTLCEKMIYQQLKPQKALNEKFAKLDATIEKGENMLKFATGILNQIIAAKE
                     EETTDDETVEKMD"
     gene            20163..20285
                     /locus_tag="ACGEUI_001095"
     CDS             20163..20285
                     /locus_tag="ACGEUI_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165936.1"
                     /translation="MMINYFAMQIEFGWITLEDVPKKYRDKVKQLVESGNIGTE"
     gene            20299..20622
                     /locus_tag="ACGEUI_001096"
     CDS             20299..20622
                     /locus_tag="ACGEUI_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165937.1"
                     /translation="MTIDVTLFAQWCVALTAIIVFLKWVSAPVKHVLDNNKKAMEALE
                     SAIVKISDDLKDNNFKWTESKNHRDRLQKVQDQHEIRIGIVEDRLISHDEQLKTLWKV
                     KEEKK"
     gene            20622..20954
                     /locus_tag="ACGEUI_001097"
     CDS             20622..20954
                     /locus_tag="ACGEUI_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFF8165938.1"
                     /translation="MEELIQVITGSALSILTIFAGIVVKLVKDYLLKKGGEKAVKIAE
                     IVARNAVEAVEQIAYDKDIKGIEKLTEAKIAVRDELSKHNVYLSDKQMEVFIEAAVKR
                     MNDSWKGQ"
     gene            20956..21720
                     /locus_tag="ACGEUI_001098"
     CDS             20956..21720
                     /locus_tag="ACGEUI_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8165939.1"
                     /translation="MATVSEVISYWRGLADTGQGYDADLAWGWQCADVTNGTTTNFFG
                     VTLWGNAIDLLDSAKAQGLEVIYDAPGINPKAGDLFVMFTYGHPYGHTGIIIADSDGY
                     TIQTIEQNVDGYSDNNGDGINDQFQVGGPARYVTRAFSDGDGYIVGWIRPPYSDTSSE
                     KQSQSQAPSGFRKLKDEVGTFEVMVPALNVRREPSLNGEIVACYQYGMTGTYDSVYVG
                     DGYIWVSYVGASGMRNYMAVGDADGDYNVNPYCKFY"
     gene            21732..22334
                     /locus_tag="ACGEUI_001099"
     CDS             21732..22334
                     /locus_tag="ACGEUI_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165940.1"
                     /translation="MGYNSTNLKQVDGGDVIKQGDTSSLFSFNLLDENNNVIDLNGKQ
                     ATIYFTRNRKTYLTKTTDVIDNKVDFTINKILEIGTYYIEVHCDGYVFPSDDSVTLDV
                     RRSGQKYVVSTDLITDTTIQKLSADIEYLKSKVTQNQHLFEQVSPQTEWTITHNLIKY
                     PSVTIVDSAGNEVFGSVEYISTTKIIVRFSAPFAGKAILN"
     gene            22345..23118
                     /locus_tag="ACGEUI_001100"
     CDS             22345..23118
                     /locus_tag="ACGEUI_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165941.1"
                     /translation="MSTFLTNINLNKNELQNAVVQNLAKEPHEPKSGQFYFDSAANNL
                     KIYNGTSWVVLDKETAESIVDLINSGKSKIDKSKVDGLEEAINQASMTGQAIATALNE
                     DTGGVTVNQNKITGLTDKLAEIATNKASATRESQDALQEAKVYADQKVAGLVDSASED
                     YNTFKELETFIKQNAKGLAAISNVAKKFSKTFGDARAKEFTITHNLKTRDVNVTIRET
                     NFPYAIVYADIKVTDENSILIATGAEVLTQNQYTVTITG"
     gene            23128..23349
                     /locus_tag="ACGEUI_001101"
     CDS             23128..23349
                     /locus_tag="ACGEUI_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165942.1"
                     /translation="MRYIGESQLSKDLVTKDYVDNKVTNLASKDDIVNLGKIKDSQTG
                     AFLEVKIVPNGQVPYDTTGMIVFERSGGK"
     gene            23349..24008
                     /locus_tag="ACGEUI_001102"
     CDS             23349..24008
                     /locus_tag="ACGEUI_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165943.1"
                     /translation="MSVQEIPDKIFYRLIEQTVEAKLVWDKDTQIGSYYISIHDKYSF
                     ESGRDVNIYKILSSTQADVYKANKYNYQSSSKPDTSVPQPKPPEQPPLKNKSLYQSIT
                     IDGLPIARVICNNQVIWSQSAEPQEVYNITAYVDASVSKLNDSAIIDYNRDFAAYGVN
                     WSRVDRVAVEVDDKIVAKLQAPVYDFGVRGGTTMSVEKWDYPSNTIDKYKNPTFKILQ
                     H"
     gene            complement(24130..>24301)
                     /locus_tag="ACGEUI_001103"
     CDS             complement(24130..>24301)
                     /locus_tag="ACGEUI_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HFF8165944.1"
                     /translation="KYETGYIKFIPKNKESFWFDFFPEPEFTQSKYLMIYKDNETLDS
                     NTSQIEVYLTTK"
BASE COUNT         8136 a         4313 c         5293 g         6559 t
ORIGIN      
        1 gactctttaa caagacaagt taaaaacatt tattttttgc aaaatacaaa agacgacatg
       61 ctgagtttta gtgatattaa cgttatgcca agaactcaag atattgagcg agaatttaat
      121 gtcaaagagt ggattggtga tgcgatagtg catgtagaga gtaagttaaa tgcgaggtaa
      181 aaatgaacat tgaagaagcg aaaagagcga taagagaact agaggcgttt aattatttcg
      241 attttaaagg taatttaatt aaacgaattg atgtattagg tatcatcgac caactcggcc
      301 aaccaaaagt ggtagtgccg aagccggtat ttgaaatgat taagtttaga aaagaacacg
      361 accaggattt aacaacaact tttgttaata tacatgctta tccagacaat gttttccatt
      421 gggtggcaaa taacggtgat ttatttgccc aagcatggct agcttatcca aatatcacca
      481 tcgaaaaaga gaagctatat acagtggaga taccaaatcc gaacagggaa ggcagaggtc
      541 atgccaagtt tgtgcttgaa agacaaggta ataaagtttt tttggtaaaa cgaaaatcaa
      601 aggattgcta ctacaataaa aacagcaatc acctcactga atccgaaatt cgtaaagatt
      661 tcgactgggc atggcgagaa ggatttgcgg aggaggtgac ggaatgaaag aaaaaacaat
      721 ttttatatca aaaaaatatg caaatgactt taacaatgac aaatataatt tgtcctctgg
      781 ctattatttt aaaaatggtg aacaacatga tattgctatt gttaaatatg gcgaacaaga
      841 ttatttaaaa aatactgatt tagcatatgt tgtatgcgat aaaatcgttg atggagaatc
      901 tataggcttc gtttatcacg gcgaatatga aacttggcgt tttaaactat taaacacgga
      961 agcaaattaa attcccgcgc aagcgctcaa gagcctgcga tggctctgtg ggggtggacc
     1021 gaaattaaaa aatagaaacg agaacctcct tacaccaaaa caaatctaaa gcgggttatc
     1081 ggtcatccgt gattatccaa ggcgtcgcta atgctttaac acgacatcgt gcgcctgtgt
     1141 caaaaaacaa aggaaagaga ggacttttct ccacaaaaca aaaagacgtc catacggaac
     1201 gcccccttgg ttaaatttaa gcttaaataa attataccac attggggggg ctttcatgac
     1261 gttttttcca gagattgata tccaaaaaac aaaatctaat gccaagcgta aattgagaga
     1321 gtatccacgt tggcgaagga tagctaatga tgtagatact caaaaagtga cagccactta
     1381 ttcctttgag ccaagacaac cgtatggaac gccaagcaag cctgttgaga gactagcgct
     1441 caaccgtgtg tcagcagaac aagagctgga tgcgattgag caagccgtca gtatgatact
     1501 agagccagag agacgcagga ttttgtatga caaatactta gcgccttata aaaaggcaga
     1561 taaggttatt tatacagaat tgtgtatgtc agagagcttt tactatgata cacttgacat
     1621 tgctttgtta gcttttgcag agctgtacag agagggtgtg ttgcttgctg agaaaggagt
     1681 ttttagctag tttttataca gtaataagat agtttataca tatttttaca tgttattata
     1741 gtattatcaa aataacaaga agagataacc ttttaatcac tgactatttt atttagtcgc
     1801 caactttaac tacaatcaaa cttgttattt tgtagcctga tggcggtaca gcgagttgag
     1861 acgacaactg ggtatgcagg ttcgattcct gccgtctcaa tcagcgccca gaagaggcgc
     1921 gacccagtat cccgaaaggc agtcacgcac tgatatactg gtttttactt ggttgatagt
     1981 catattccta ccaatgacga ttgataaaaa agatacatag atcggctgta tagtctacgt
     2041 gtacgtattc acaacagatt gactcatttg attaaccgca agtaaaacaa gggtcgcaac
     2101 cttgcctgtg gttagtaggc ttaatggtcg tttatgcacg ttcgattcgt gcaaagcctg
     2161 tttgataaat agaagtgtcc cagaatgggg taggcaatag gcttagcatt catttgctat
     2221 ttatctatgg ttaaccaatt agtcatcaca ttgtggtgac ttttttattt ccgaaaattt
     2281 ccgaaaggag gaacatattt tggcaagagc aaataaggca aaaatgcttg atgctattga
     2341 gtttaaatta ttaaatccaa ccgctaatca agcacaagtt gcggaaataa tcggtatcac
     2401 agacagacag ctacgaaatt gggaaaatga cccagaagat aaatcgggtt ggaataaatt
     2461 aaacgatgaa atcgcagaaa aagcttttgg caaatatgct ctttctgcga ttggaacgct
     2521 tgttaatcta agcaaaaacg caaaatcaga attggtaaga ctacaagctt ctcaagatat
     2581 ccttgaccga gcaggttata aaccgacaga caagcaggaa atcagcatag acgagcctat
     2641 cgtacttgct aattcatggg tgcaagacaa tggtagttga tttagctgat attatcccaa
     2701 ttggttttaa gcctgtcgtg caagcgactt ggaatcctca aatactaaat atcgcttgca
     2761 aaggcggacg tggttctggt aaatcatcta atattgcttt tattatctcg aggttaataa
     2821 tacagtatcc tgtcaatgcg gtttgtatcc gtaaaacaga taatactctg gagcagtctg
     2881 tttatgagca aattaaatgg gctatatctg agcaagggct agagcgttac tttaaattta
     2941 acaaatcacc tctaaggata acgtacatcc ctagaggtaa ttacattgtc tttagaggag
     3001 cacaaaatcc tgaacgtatc aagtccttaa aagatagtcg ttttcctttt gctatcggtt
     3061 ggatcgagga gcttgcggaa tttaaaaccg aggacgaagt caaaactatc actaactccc
     3121 tattgcgtgg ggaactcggt gatggtcttt tttataaatt tttttataca tacaatccac
     3181 ctaaacgtaa acagtcttgg gttaacaaaa aatatgagag ccaatttcag ccgtctaata
     3241 cgtttgtaca cgcatctacg tataaagata atccttttat tgccaaagag tttatagccg
     3301 aggcagaagc cacgagagag cgttcagaac gtcgttatcg ttgggagtat ttaggagagg
     3361 ccattggttc gggtgttgtt ccatttgaca acttacgctt cgaacgtatt actgacgaac
     3421 aagtagccga tttcgataat atccgtaacg gtattgacta tggttatgca actgacccgc
     3481 ttgcttttgt acgttggcat tatgataaaa agaaaaatgg tatctatgct attgacgagt
     3541 attatggtca gaagatatca aacaggcagc tggctaagtg gttgacgact aaaggatatc
     3601 agagtgatga gatgtttgct gaaagtgctg agcctaaaag taatgctgag cttaaaaacg
     3661 agtttggcat caaacggata aaaggcgtta aaaaaggacc agattctgtc gagtttgggg
     3721 agcgctggtt agatgactta gattttatct gtatagaccc aaaacggaca ccaaatatcg
     3781 ctcgagagtt tgaaaatatc gactatcaag tcgatagaga tggcaaccct aaacctcgtt
     3841 tagaagacaa ggataaccat gcgatagatg cgacaaggta tgctatgtca gacgacatga
     3901 gagcgactaa aaccatcgtc aaaacattta aaggaggtat ctagtgccag aattatttat
     3961 tgtgccatca gatacagaga tgacaaaaga tttgttaagc gagttaatcc aaaaacacag
     4021 gtcgtttaac cttagttatt cagcgtataa gcagctatat gagggtgatc atgctatttt
     4081 gcaacaaaaa caaaaagagc aatacaagcc tgataatcgc ttggttgtta attttgcaaa
     4141 atacatcgtt gatacattta acggctattt tatcggtgtg ccagtccaaa cgagccacga
     4201 gaataaacaa gtcagcaatt atttagagtt attagacgga tacaatgatc aagacgataa
     4261 caacgcagaa ctatcaaaga tttgcagtat ttacggacat ggctatgaat tagtttttaa
     4321 tgacgagaat gcagaggttg gaattactta tctgacgcct cttgaaggat ttattgtcta
     4381 tgacgactct attagacaaa agccgttatt tgctgtgcgc tatttttata ataaggacgg
     4441 tgttttagag gggtcttatt cagacgccag caaaatcact tattttaaag atggcgaaaa
     4501 agaccttgaa atcggggaga gcgatccaca tccgtttgat ggtgtgccaa tgattgagta
     4561 tgtcgaaaac gaagagcggc aaagcttgct agctagcgtt gtgacattaa ttaatgcgtt
     4621 taataaagct atctccgaaa aagccaatga cgttgagtat ttcgcagacg cttatcttaa
     4681 aattttgggt gctgagctag acgacgagac attaaagtct cttagagaca cccgcattat
     4741 caatctaaaa gacaccgatg cacagcaatt aactgttgag tttttacaaa agccagatgc
     4801 cgatgcaacg caagagcatt tgctcgaccg actagagaac ttaattttta ggactgctat
     4861 ggttgctaat atcagtgacg aatcgttcgg tacagctagc ggcatcgctt tacgttatcg
     4921 tttgcaagct atggataact tagctaagac aaaagagcgt aagtttatga gcgggatgaa
     4981 ccgcagatat aagcttattg caagctatcc tacgtctaag ataggaccta aagattggat
     5041 tggcattaag tataaattta ctcgcaatct accagcaaat ctcttagagg agtctcaaat
     5101 tgcaggcaat ttagcgggga ttgtctcgga agaaacacag gtcggtgtgc tatctattgt
     5161 ggaaaaccca caaaaagaaa tcgaaaagaa aaatagcgac aggccaacct taattagtcg
     5221 aaaagcagga gggctaaatg gccaagacac cacaacagta ttggaatgag cgccagaagc
     5281 aactatgggc taatttagag acgtcagagc gcgctttaca gactaagctt agcaaatatt
     5341 acgctgatga agcaaagtcc ttagaaaagg aaataggggc ttattttagc aagtatggaa
     5401 aagacaacgt tattgaatat cgaaatcttt tgcagcagtt atctaaagct gataaagact
     5461 tgctttatcg agattgtgag cgatttgcta aaaaatatcc acaacgtgct gatttaatgc
     5521 ctgtcaggac ctctatttat aaattagata gactgcaagg actagaatta agcattaaaa
     5581 tacaacagtt ggaaataggt gctatcgaag aggcggaatt gactaagcat ttaacgacag
     5641 cctttaaaaa aggttatcaa gaaaccgcta aaacaattgg tttccaagcg gataaagtat
     5701 ccgctgagct gtttgttaac aatgattgga caggcaaagg tgattttagc tcaagtattt
     5761 ggtctaacaa agataagctt gtcgattatc taacaaatga ctttaagacg gcaattatca
     5821 ggggagatag ctttgataaa gtcgttaaac agatgtcaga gcgttttact gtacgttcgc
     5881 agtcggatat tacacggctg atcatgacag aagggactta tgtcaataat caagcgatga
     5941 tggcaccgtt tgaggatagt gacgactttg aggagtacga atttgtctcg gttttagata
     6001 gctctactag ctcaatttgc agaggattag acggtcataa atttaaactt aaagatagac
     6061 aggttggggt taacttcccg cccatgcacg ctaactgtcg cagcacattt gctatggtta
     6121 ttccagatga ttatttagag aggtaaaaat ggatgaataa acgcattaag aaaaaacgta
     6181 aattagaaac agctattgtg atgattgttg cagagaacgc catgcaagca gaagcactca
     6241 ggaatcaaaa caaagaaatc atggagttaa aatcaatcgt tcaacgaaac gctctggcaa
     6301 caaacgaaga gttagcgact gttaaagctg ctactttaga taaccaatca gccatcaagt
     6361 caatcggcga tgatgttgac tacatcaagc aaaattacaa acggaagtgg aggaagaaat
     6421 aatatggtgt tagagcaagc tgaactagaa atggttagaa atcaacgtct ggttatgatt
     6481 aaccctgaaa tggtaactat cacaaaaaca gaatatgatc tgttgattga aataaaaaac
     6541 aaatttttag agtcgtagca atacggcttt ttattatgtc ctgtcgcatg acaaaaaact
     6601 aggcaaataa aaatctaaag caatgcgctg gggcttttga gcaatggcgt ggggcaggag
     6661 gaaaaaatgg aaaacgaaga aatcctagaa caatctggtg cacaagaaga agctaaggag
     6721 caaacgtttg acgatatttt gtcagaccct aaaaagcaag ctgaatttga caagcgtgtg
     6781 gctaaggcta ttgatactgc acgaaataaa tgggtggctg agactgaaga aaaagaaaac
     6841 gaagctaaga gacttgcaaa aatgaatgct gaacaaaaag cacagcacga aaaagcaaaa
     6901 cttgaagcac gcattgcaga gctagaagct gaacgtacat tgtcagaaat gaagagtgct
     6961 gctcgcacaa tgctatcaga agctaatatc aacatctctg acgcactttt atcgcaattg
     7021 gtatctactg atgcagataa aaccaaaaat gcagtagaag cgttttctga ggcattttcc
     7081 gaagcaatcg aaaaagaagt caaagaacgc cttaaatcgc caacacctaa aaaatcaaat
     7141 ggcaatactg gactgacaaa agaacaaatt ttagcagtca aagatactgc ggaacgacaa
     7201 aaactcattg ccgaaaatat cggcctattc caataaaaca ggagaaatta acacatggta
     7261 acatcacgta cttacccaga agaaaatcta atcaaatcaa cagaccttaa ataccctatc
     7321 actattgatg tcacaaacaa attccaagaa aatatctcga aattacttga aatgctcggg
     7381 gttactcgta aaatctcggt atctgagggt atgaccctta agacatacgc tggttatgat
     7441 gtcacattag cagaaggtaa cgtcccagaa ggcgaagtta tcccgttatc aaaagtagag
     7501 cgcaaaatac actctgagaa aaaaatcgaa cttaaaaaat atcgtaaagc aacaaccggc
     7561 gaagacatcc aaatgtatgg ctctaacgaa gctgtaacaa atactgacaa cgcacttgtt
     7621 cgtcagttgc aaaagaaaat ccgcacagac tttgtgactg ctcttaaaac aggtacagga
     7681 acacaagatg ctcttggtgc aggattgcaa ggggctttag cttctgcttg gggtaaactg
     7741 caagtgctct ttgaagatta tggttcagag cgtgcgattg tgtttgctaa tagccttgat
     7801 gtcgctgaat acatcgctaa agctggcatc actactcaaa ccgcattcgg attgacttac
     7861 cttgtcgatt ttacagggac ggtcattatc tcgacaaacg atgtcacaaa aggggaaatc
     7921 tgggcgacag taccagaaaa tatcatcttt gcttacatca atccaaataa ttctgaatta
     7981 gctaaagaat ttaatcttta cggtgatcca accggctaca ttggaatgaa tcacttccaa
     8041 gaaaatacaa cgcttactat ccaaacgttg cttgtatctg gtatgttgat gtacccagaa
     8101 cgtattgacg ggattgttaa agtaactctt acaccaggcg tctaatgggg gtagcttatg
     8161 gcttatagag taaaagcacg tttttttgat ttgcttgaca atagcttttt gtacgaggtt
     8221 ggcgacagct ttcctcgcaa aggctatgag ccaagcaaag agcgcttaga aagcttgtta
     8281 tctagcaaca atacagaaca taaaccattt attgaggtgt caggtaatgg caatagctta
     8341 gatggtttaa aagtggatga gttaaaagct aaagcggcag agctaggaat cgaactccca
     8401 agtaatgcta aaaaagctga aatcatcgaa ttactgcaag cacacatcta gaaagtaggt
     8461 tgacatggac gaaacaaaac aagcaattat tgatcgtgtc cgtgtccgtt tagcagacga
     8521 aacgtcttta aaagaggagt tattggagga gttaacgcaa acggctatcg accgtattaa
     8581 tttaaaagtc ggcgatgtcg tttttaaccc tctttttaat tccatcgctg ttgatgttgt
     8641 tgtcaaaatg tatcgtcgta tgtattttga aggaattgac acggaaaaag cagacacaat
     8701 atcaactaaa tttattgaaa acgttttggc agaatatggc gaggagttag cgtcatataa
     8761 aaaggaccgt ctagccatct tgaataaaaa ggtggtgcgg tttttatgat gtttgttaat
     8821 tttgacttag taacatcgca aaaaacggga gaaaaagaca gactcggcaa cgacatcacg
     8881 aaaaatgttg tcaaaagagt tgctaaaggt cgttttactg aatggtcggc tgatgacgtg
     8941 tccttatacg gtcgagattt aacgtctagc gcacgcaaat tgctgactaa tcaagttagc
     9001 aaggcggaag ccaaacaagc gtcacacgtt gtaatagacg gctcaaaata caaagttgaa
     9061 tccgttaaag atcttggtag atggagacta ctcgtcatta aagggtatcg cttatgagga
     9121 tgtcaatgac aggtatgcca gccttagagg taaaactaag gtcaatgagc gagaagcgct
     9181 gggatagggt tgccaacaaa aaccttacag agatgtttaa cagagcagca agacctcctg
     9241 gtacaccgat tggtaaaaac actaaaaggc ataagtctgg cgagttgttg cgctctagac
     9301 gtctcaaaaa ggtaaactca tcaaaagatg ttatcacagg taactttggt tatatcaagg
     9361 actatgcacc tcatgtcgag tatggacaca ggattgtccg caacggcaaa caggttggct
     9421 atgcaaatgg tacgaaatac ctgtttaata acgtcaaaaa acagcgtgaa atttataggc
     9481 aagacatgct aaacgaattg aggagatgac atgttaaaaa agttgggact ggttgattta
     9541 cacgcctcaa ttaaacaaaa aatcgaagat aagacagggt tgatggctta tgatcatgtc
     9601 ccagaagaca tgccatcgcc cttttatttt atcgaggttg tggataaacg gccagaagac
     9661 acaaaggtca tgtggtgcga agtgtttacg gtttggatac atgccatagc ggaagctgga
     9721 aaaagcaaaa tcgctattta tgacatgatt gaaaaactcg aagaagcgct cacagaagaa
     9781 ttagttttgc cagaagaaat cgacattttg agacaatcag aagtcggcat gcagtcctta
     9841 caagaggatg agactggcga aatgcatgca attgtagcct acgaaatcaa ggtttcgtac
     9901 ggttttaaag taaaagttta aaaggagaaa taatggtggc atttgaccaa aatgtttatt
     9961 gtaattttga cacatcagca acaaaagcta ttgcaggtaa agacattatc ttggcaatct
    10021 ttgataaaac tggtgcaaat ttacttgcta ttagtggtca gcaagggtta acaatcaacc
    10081 gtacggctga ctcaattgaa gtatcgtcta aagatacaaa gggtggatgg aaatctaaaa
    10141 tccctggcat gaaagaatgg tctatcgata atgatggtct ttacgtgccg tcagacgaga
    10201 ctcacaaaca attgggcgaa gcttttgaaa agagcgagtt tgtttgtatt aaagttatta
    10261 acggtaaaac caaaaaaggc atgtttggag ggcttgcgtc ggtaagtgat tatagcctag
    10321 aagctcctta cgatgatggt atgacatact caatcagtct cgaaggcaat ggagcattga
    10381 ttgatttatc aacattatca tcagaagatg ctgaaaaagt aacggctatg cctagttaat
    10441 gagaggaatg aatggataaa gaattaatta ttattgacga aaaacaatac gaactaaagt
    10501 acaacgaaaa aaccattgaa accgtcgaag ctttgacagg taaagcgttt atggatgtta
    10561 ttgtgaataa caaaggtatg ttgtcgctat ctatgttgcg tcagtacttt gctaacgctc
    10621 tttatgcggt agaaggcggt cgtgtgtcta gcgagcaagg ctctaatatt tttactaaag
    10681 tgttaaatac aaaaggttat gcgtatgtaa atatgctagt gatcaacaca atccagcgtg
    10741 attgcccttt tttcttcctc ggcgcttagt cgactttgag tatctcggcg ggtctggcga
    10801 tagcacagat gtagagtatg acagattagc aagtcagtat cacaaagata ttgactttgc
    10861 tttttatttt gtcaattttg ggactacaaa atccgaattt ttggaattaa ccagacgaga
    10921 aaaagccttt atcagaaaag cttgggaaga taaacaggtg cgagaaagtg agcttatgcg
    10981 caacgctgtt ctaaatgcag tcagtaacgc tatgcgcaaa aaaagcgcta aatttgttga
    11041 tttgtggaaa cgtcagcaac agcctgcgaa catggaaatc gtcgaagcgc atttggaaat
    11101 cattaataaa aacatcgctg atgaaggcaa atattgggtt gatttagtct atcaagctaa
    11161 caacatgaca aaaccgagcg agggggcaga aaatggctga ttatacttta ggagtacaaa
    11221 taacaggtga cgcctctaaa atgcaaaaag cgtttgaaaa agcgcaagaa caagctgaaa
    11281 aattaaagga aaaaattaaa gactcaaaca acgaatctgg caattctttt gatggtttga
    11341 ccactaaaat tgacgcaata tctggcaagc tgtcagctta cggagataaa ataagctcta
    11401 ttggcaaaaa catgacaacc atgtttacat taccagcggt tgctggtata acggcagttg
    11461 ttaaagagta tgcaaatctg gaacagtcaa ttggtggtgt tgagacttta tttaaaaagt
    11521 ctgccaaagc tgtgcaaaaa aatgcaaata cagcgtttag acgagctggt gtatctgcta
    11581 atgagtacat ggagcaagta actagctttt ctgctagtct aatttcatct cttggcggag
    11641 atacagcaaa ggctgtcggt gttgctgata ctgccatggt cgacatgtct gacaacgcta
    11701 ataaatttgg tacaaacatc gggttaatcc aaaatgccta tcaaggcttt gctaaaggca
    11761 attacacgat gttagataac cttagattgg gttttggtgg gacgaaagag gagatgcaaa
    11821 gattgctgga cgaagctggt aagctaagcg gcatggaatt tgacatcaat aacttttcgg
    11881 acattataca agcaatccac attatccaag ataatttagg cgtaacagga acgacagcta
    11941 gagaagcgtc agaaactatt agcggctcat tttcgcaagc aaaagcgtca gtaatcgatt
    12001 tcatgggttc tcttggcgga gatggcagtg acgtagatgc aaaaatgcaa gcaatgatac
    12061 agtcgattac tgtgtttgca gataatgtca aagaggcttt ggggcgtatt tgggacaatt
    12121 tgcctttaca agagtatcaa aaaaacctct tgcttatagc tgtcgttggc ggacctatcc
    12181 ttgttgtggt cgggactatt tttggagcta ttgctaatat catgactgtt gttagcggta
    12241 ttttaaccgt tttaggcatg atgacgccaa taggatggat catagcagca gccatagcgg
    12301 ctattgtcgc agctgtcgtt ttttgggacg atattgttgc agaggtcggc caaaaatttg
    12361 agttagtcaa aataatcggc atggcgatgt gggaaagttt taaagatggt gtatctagtt
    12421 ttggcaagtc aatccaaaat gtcgttggca aaattggcgg atggttcgcc gaagcgtgga
    12481 atgctgcagc cgacacagcc actagaattt ttactaaggt tggggatacg attagtggca
    12541 tctttagcgg cgttgtttct ggcattaaaa gtttgatcaa cggagcaatc ggtggcatca
    12601 ataaggttat tggcattacc aataaactgc ccggtgtcaa cataccaaaa atcccatatc
    12661 tcgccagagg tacatctgac tggcaaggcg gttttgctcg tattaacgag ggcggtcgtg
    12721 gagaattagt tcatatgcca aacggggcaa ccgttgtccc gcacgatgtc agcatgaaat
    12781 acgctcgaga atctgctaaa gctaattctg gtgttgctta tatggatagt aatgatgaga
    12841 ttgccaaaag tgctttgaaa ctagctaacg atgctgttaa acgccctgtt gtgttaaata
    12901 tcaacggccg tgaggtcgct aaaacgacag gcggagatat gagagattat ctaaatagtc
    12961 gagatatgac attaaaacga ttgagaggtg aggtttagat ggctacaatg acttttaatg
    13021 atgtggactt atctagcctc atcactatca tgtctattaa tcgcgacatc ggaaacgagc
    13081 gacaagcaga tactaatgat gctccgttca tcggattgca cattaaaaag attaaaacta
    13141 gcgcaaaaat cattacagtt gacttttatt taaaagacag gacaaacgag tacgcattaa
    13201 accagctaaa acataaacta gctggtattt ttaatgttaa cgagcaagtc aaagtaactt
    13261 ttagcgacga accagataaa tattatctag ctataccgat aaataaaata tcggctagcg
    13321 acccaatcgc ttggctaagc cttgtgagct tggaattact tgttcctgac ggcgtggccc
    13381 attctgtgtc atacaaaaaa atcacgaatt acagacaaga tggcaagaag ctactgattg
    13441 acattacaaa caacggtaat gttgatgcgc atccgatcat tactgttaag cacaacgccg
    13501 agaatggtta tttagctttt gtaaataaat catccgtttt cgaggtcgga gacagggaaa
    13561 tagcagacgc tgaaatccgt gaaaagtcgg tcgtcgctta tgattttaaa gacgaaagaa
    13621 tcgtcaatgc cttaacagca ggtaaaaaga atgttgctat cttaaatgac tcgacacaag
    13681 ttcttgataa aaatttatta ctaaataccg tctggggaag aaaacatctc gagttagaag
    13741 gaagtgttgc gccaagtggc aaccatgctg gaacgttaac gtttgatatt cctgacggag
    13801 gtagcttata tgactatctt tggtggagac aaattttttg ggttgggact gtttcccagc
    13861 ttggctttat caaaatcatg gtatcagacg aaaacggaca ttttttatat ggagtagaaa
    13921 ctataaaaag aacgttagga acaaaaacag agtataactt tttggtcact aatggaaaag
    13981 gtggatataa aataactgat ttacgttgga cttttgatgc tactcacttc gaaacacaaa
    14041 acccctttaa cgaacccaga ggttggtctg atatgacaag ggctgatgac actgtatctg
    14101 ttttttggtg gggttctcaa aacaagcgag tattcccaga attaaaaggc aaaaaatcta
    14161 aacaggtaca catcgcaatc ggcactattc aaggcagtcc tttagtaact cacatgtata
    14221 ttgatggatt ttattatcgc aaggacaaag taccgtacga atttaatatt ccgaatgctt
    14281 tcggggcagg aacagaagtc gttattaatg ccgaaaatga cacagtttta gttgataaca
    14341 ttccaaaagc taacatcatc gccgatggct tttttagttt tcctaaaatt cctcctggaa
    14401 catcaacatt agaggtttat tcctctagtt ggtctaccgc caacccagat attacgctaa
    14461 attttgaaga aaggtggcta taattgctta taacaatcca caacgcaaat ttagaaaaag
    14521 ttgcttatat tgataacgat aagcaagaca ccttgaatta ttatgacgac aaattttcac
    14581 agtatttaaa tacggccaat tcgacatttg agtttacggt ttataagcga ggaattaagt
    14641 ccgacacagt caaagagaaa gcctatctga cacttacaga aaggtctttt gtgtcgttta
    14701 aatataatgg caagtcttat ctgtttaacg taatgacaac agacgagaca gacatcgaga
    14761 tacgttgtta ctgcgagaac ctcaatctcg agttgctcaa tgagtatgca ggaccttata
    14821 aagcggcaac tcagatgtct tttgtcgatt attgtaattt gtttggcatc cttaaaaacg
    14881 gtgctattac cattggcact aatgagacaa ccgaccaaaa acgcacaatc gaatggactg
    14941 gtcaagatac caatcttaaa cgcttgttat ctatcgccaa taattttgat gcggaaatag
    15001 aatttgtaac gcatcttaaa aacgattcta gtcttaaatc gtttgtcatg aatgtctaca
    15061 agaagaacga cgctactaat cagggcgttg gtcgtagacg agatgatatt attttgcaat
    15121 atggcaaaaa tatcgagagt gtcagacgta agattaataa aacaggcatt tacaatgcta
    15181 taagaccaag cggtaaaata acaacgacta caaccacaac gactgctaaa caaggctctg
    15241 tgcaaacagg gtctgttttg tggtctggcg gtaacttgac ttatgcaggt catgtaatgc
    15301 aatcatctgt tgttaacact attttaagcc tatgtagcaa atacaaactt ttaccatctg
    15361 gtgtttttag ccagctatac cttgaatcgt tttggggaga taccccagtc ggaagagccg
    15421 ataataactg gggtggtatc acttggactg gcgcaacaac taggccaagc ggaataaatg
    15481 tctcccaagg gcagtctcgt gctgaaggtg gttattataa ccattacgcc agtgttgatg
    15541 actacttgaa agattacgct tacctcttgg ccgagcaagg catttatgcc gtaaaaggta
    15601 agctaaccat tgatgagtac acaagaggtc tgtttagggt cggtggcgca acatatgatt
    15661 atgctgcagc tggatatgat cattatgcac ctttgatgcg agacatcaga gcaggtatta
    15721 accgtaataa taacggcgct atggataacg tcgataacca atttaaaaat ggtggctcga
    15781 ctagtcaaaa cactactcag atagctgcta aaacaaaagc ggtacttgcg gaagcaaatg
    15841 gactgaaagg tcaacgagta ggctctggtc agtgctatgc gttagctgct tggtacgcca
    15901 tgaaattaga tggtccaggt ctgaacggtg gtgtaactag ttttagaggg cttattggtg
    15961 ctggtgctgc cgctgctcag attggtacgg attacaactg gggtcagttt ggctggaaag
    16021 ttgtacaacc gaataaagtc gcagacttaa tcacaggctc gattgttaac atcagagcaa
    16081 acgctggcag tcctgttttt acaggcgctt gggggcatac tgttgttgtt aaatctctat
    16141 ctggagatac actcacagta ttagagcaaa actataacaa cgtgcaaaca gtccaagagc
    16201 atacatatag cgctagcgct tatttatcag ttgtacagac agtctgttat ccgccagaaa
    16261 tcgttcaagg aaaacgcgtc gaaggtactg cacaagcaga acaaccgcaa tctgaaacga
    16321 ccacaatatc tgaagaaaaa gaggtcttaa ttaatccatc actttatcgt gagtggaaaa
    16381 acgaagcggg acaagtcgag ttttacgtta aaaacagtat gctctacgca cctctgtcaa
    16441 aatctcttta cccgtcagcg tttactggca tcgagacgga cgataactgg atacgaaaag
    16501 acttagatgt tgacacagag agcgaggaaa agcttatctc tgttgctctc gcagacctga
    16561 gaaaacattg ttatccagcg gtgacttatg aagtatctgg ttttattggt gatttagata
    16621 ttggtgacac tatcaaaatc aatgacccag aatatacccc aagtttaatt ttagaagcaa
    16681 gggttagcga gcaacacatc tcgtttacag agcctaatca aaataagaca gtatttgata
    16741 attacagagc tttagagagc aaagtctcac aaggtttaat tgatcgcatg aatgaactag
    16801 cagaagctgc taaaccttac gacttgcggt taatgacaga taacggaaat gtgtttttaa
    16861 atggcgaagg tcgtacgatt ttaaccgctg aactttggaa aggtaacaaa aagtttgatg
    16921 caagctatca atttaaacgt gatggtcaat tagtcggcgc tggattgcag ttggcagttg
    16981 acgctaagga tgtaccagct gatagacctc taatcattac tgttgaggct tatttaaata
    17041 atgagctgat tgcaagtaaa cagattacgt ttactaactc gcttggagaa caaggaccag
    17101 ctggacgtgg gattgtctct acagaggact attacttagc gtcaccaaat cgtacaggtg
    17161 tcacatctgc aacatctggt tggactaaga cgcctcagga aattactgag actaataaat
    17221 atcattggta ttatcacgtt gatgtttatt cagatggcac tcgaaaagag actacaccgg
    17281 ctattattgg tgtttacggc gataaaggtt cagatggcaa gcaaggcaaa cgtggggaga
    17341 tgggaccatc aggacctcct ggcgctttag ataaaaagca actacaagac atcaataata
    17401 aaattgatgg caaagcagac cagaatttga ccatcgagca aatcaataaa ttagccgagt
    17461 tacaatctat tgccaacgct gagttacaag caaaggctag catcgacgct ttagctagct
    17521 tacaaaagca agtacagtct gcaatagcgg caatgaatgc tagccaaaag ttatcagagc
    17581 aagaccttat tacagctagt caacgggcta tcaaagcaac caacgacatc ttagacttaa
    17641 aagagcaatg gaattttatt gacaattata tgtcagcgtc tgaagaggga cttattatcg
    17701 gctctaaaga cggtacaagt tccgtgcgtg ttgccaaaga ccgtattgcc ttttactcag
    17761 ctggcgcaga agtcgcttcg attactggtg gtatgctcaa aattgataat ggtatgtttg
    17821 tggctacttt gcaagttgga cattttcgcg aggagatgta caaagttgat ggggtagata
    17881 aacacataaa tgttacaaga tattacgaaa cgattgtggg gtgattaatt ggcagagttt
    17941 tggtcaaaca atgatagagg ttaccgcatc aaacttacga ttgatcaagt atcccaaaat
    18001 acattagcaa acagtagcca agtgagggtt aaacttgatt tgctaaatac aacaacaact
    18061 tttactcaat atagttgttc tgcatatgtt gacttaaacg gtcagcggat agactggtca
    18121 gactcaccat cagtacttag atataactca acaatttctc tgatcgatag aacaataaca
    18181 gttaatcata atgctgatgg tactaaatca tttggattta tcgctagctt tagcggctct
    18241 ggtggatggt ctccaggtac tttgactgtt ggtagtggta cttttacact tactaccatc
    18301 ccacgctcaa gctccgttgt tgtttcttct ggtactattg gcagtgccat cactatcaac
    18361 atcaatagac aaagctctag ttttaagcac aacttgagat accagtgggg taataaaaca
    18421 ggtactattg caagcgatgt ggatacttcc gccgtttgga ctatccctct tgattttgct
    18481 agtgacatcc caaactcaac aagtggtagc ggcactatct atgtagatac ctacaatgac
    18541 tcaacgctaa ctggtactca acaagtgcca tttaccgtaa ctgtaccaga cagtatgaag
    18601 ccaacattat caagtattag cttgagtgat gctcataccg ttgcaggcaa cgtagtatca
    18661 agtgcggatt attttatcca agtatactct gacattagag tcaattttga atcggcaagt
    18721 ggctcttatg gttcgacaat caaaggctat tatgctgaga ttgtcgataa aggacagtct
    18781 actgaccaaa acggcggaac gctaggcaac atgctttatg atggtcaaat cactatcaga
    18841 gcaaaagtta ttgatagtcg aggtcgtgaa tcgcaaccca tggataaaac agtcacggtg
    18901 ctcaagtact ttccgccagc tttgtcgttt gatgttgcaa ggtctggtta tggttctgac
    18961 acgctaacag ttacaagacg agcgtctatc gctccgttaa gtgttttcgg aacacaaaaa
    19021 aataccatga cgcttagttt tagtgttgca gaattaggtt ctagttattt ttctgctaat
    19081 aacggctctg caagtggggc atgggcaaat gtttctagtt tggtaaactc gtctgccaat
    19141 ctttatggtg ctttctcgcc gacaaagtct tataccgtca aaggtatctt gtctgacaaa
    19201 tttagtcgca cagagtttac gtttgacgtc ggaacagaat cagttgtgat gtcaatcgct
    19261 aaaaatggca tcggattcca aaaaatttgg gaaaagggtg ctattgacgc taaaggagat
    19321 gcttatatta gcggtaagtt atttgtcaat aatacagagg ttaaaccgtc gtttgacaag
    19381 acagagattt taaacatggt ttatcctgtc ggagctattt acatgagcac atcatcagcc
    19441 aatccatcaa catttatcgg cggcacttgg caaagatatg cacaaggtag gactattgtc
    19501 ggtgtctccg aaaacgagac ggaatttaac tacgttggta aaactggtgg agccaagact
    19561 catacattaa ccaatgaaga gatgccaagt cacagtcacg gggataagac tattagctcg
    19621 ggtggtagac ctatctcatc aaatgctggt tgggacaata caaatgttgg tttatataaa
    19681 tcaacagatt acaatcaaat aaacgctttt aacaagtctt ccggaggtga tcaaccacat
    19741 aacaacttac aaccatacat cacaacgtac atttggctga gaacagctta gaggagaatt
    19801 tatgttagtt atcacatcaa attacccaga acaaatgcct gatggttccg tatctggtgc
    19861 gaaggtgatg cttgacggga ttgatgatca cgctggttgg catatcccgt tgacattact
    19921 aaaagaatat ttagacaaac ctcaaagcga ggttatgaca ctttgcgaaa agatgattta
    19981 tcaacaatta aaaccacaaa aagcattaaa cgaaaagttt gctaaactcg atgcaaccat
    20041 tgaaaagggt gaaaatatgc ttaaatttgc gactggaatt ttaaatcaaa ttatcgcagc
    20101 aaaagaagag gaaacaacag atgatgaaac tgttgaaaaa atggattaaa aggagatata
    20161 tcatgatgat taattatttc gcaatgcaaa ttgagtttgg ttggattact ttggaagatg
    20221 tacctaaaaa atatcgtgac aaagtaaaac agcttgttga atcagggaat atcggaacag
    20281 aataacgaga ggccttatat gacgatagat gttacactat ttgcgcaatg gtgtgtagcg
    20341 cttactgcaa taatagtatt tttaaaatgg gtgtctgcac ctgtcaaaca cgttttagac
    20401 aataataaaa aggctatgga agccttggaa agtgctatcg tcaaaatatc tgacgactta
    20461 aaagacaaca attttaaatg gacagaatcc aaaaaccatc gtgacagact acaaaaagtc
    20521 caagatcaac acgaaatcag aatcggaatc gtagaagata gacttattag tcatgacgag
    20581 cagctaaaaa cgctctggaa agtgaaagag gaaaagaaat aatggaagaa ttaatacaag
    20641 ttatcactgg ctcagcacta tcaattttga caatctttgc aggtattgta gtcaagttag
    20701 tcaaagatta tttgcttaaa aagggcggag aaaaagcggt caaaatcgcc gaaattgtgg
    20761 ctcgcaatgc cgtggaagca gttgagcaga ttgcttatga caaagacatc aaaggcattg
    20821 aaaaactgac agaagctaaa attgcggttc gtgacgaact gtctaaacac aatgtctatt
    20881 tgtcagacaa acaaatggaa gtctttattg aagcggctgt taaacgcatg aacgacagtt
    20941 ggaaaggtca ataatatggc aacagttagc gaagtaatta gctattggcg tggattagct
    21001 gatacaggtc aaggttatga tgccgacctt gcgtggggct ggcaatgtgc tgatgtgact
    21061 aatgggacaa ctactaactt ttttggagtg acgctttggg gaaatgcgat tgacttacta
    21121 gatagcgcga aggcgcaagg gttagaggtt atctatgacg cccctggaat aaatcccaaa
    21181 gctggtgatt tgtttgtgat gtttacttac ggacatcctt atgggcatac aggaattatc
    21241 attgctgata gcgacgggta cactatccag acgattgagc aaaatgttga tggctactca
    21301 gacaataacg gtgacggcat taacgatcag tttcaagttg gcggaccagc tcgctatgtc
    21361 actcgtgcct tttcagacgg cgacgggtat atcgtgggtt ggattagacc gccttatagc
    21421 gatacctcat ctgaaaagca atcacaatca caagcgccat caggattccg gaaattaaaa
    21481 gacgaggtcg gaacatttga ggttatggtc ccagcgctaa acgttcgccg tgaaccaagc
    21541 ttaaatggcg agattgtagc ttgttaccaa tacggcatga caggaactta tgattctgtc
    21601 tatgttggcg atgggtatat ttgggtgtct tatgtcggtg catctggcat gagaaattat
    21661 atggctgttg gagacgctga tggcgattac aatgtcaatc catattgtaa attttattaa
    21721 aagggggtta tatgggatat aactcaacca accttaaaca ggttgacggt ggcgatgtta
    21781 tcaaacaagg tgatacatcg tcacttttta gttttaattt gttggacgaa aacaacaacg
    21841 ttattgattt aaatggcaaa caagcgacta tttactttac acgtaatcgc aaaacctatc
    21901 taaccaaaac aactgatgtt attgacaaca aagttgattt tacgattaat aagattttgg
    21961 agattgggac atactatatc gaagtgcact gcgatggcta tgtctttccg tctgacgaca
    22021 gcgtaacgct tgatgttaga cgttctgggc aaaaatatgt cgttagcact gatttaatca
    22081 cagacacaac tatccaaaaa ctatcagctg atattgagta tttaaaatca aaagtgactc
    22141 aaaatcagca tctatttgag caagtgtcac cacaaacaga atggaccatt acacataatt
    22201 tgattaagta tccatctgtg acaattgttg atagtgcagg taatgaggtt tttggaagtg
    22261 tcgagtacat tagcacaaca aaaatcatcg ttagattctc agcgccattt gctggcaaag
    22321 ccattttaaa ttaaaggaga ttacatgagt acatttttaa caaatatcaa ccttaataaa
    22381 aacgagttgc aaaatgcagt cgtacaaaat ttagctaaag aaccgcatga accaaaatca
    22441 ggacaatttt attttgacag tgctgctaat aatctaaaaa tctataatgg gacatcttgg
    22501 gttgttttag acaaggaaac agcagaaagc attgttgacc ttattaattc tggtaaatcc
    22561 aaaatcgata aatcaaaagt cgatggactt gaagaagcaa ttaatcaagc gtctatgact
    22621 ggtcaagcta tcgcgaccgc tttaaacgaa gatactggtg gcgttactgt aaaccaaaat
    22681 aaaattacag ggcttacaga taaactagct gaaattgcga ctaacaaagc gtcagcgact
    22741 cgggagtcac aagatgcttt gcaagaagcc aaggtgtatg ctgaccaaaa agtagccgga
    22801 cttgttgata gtgcatcaga agattacaac acgtttaaag agctagaaac ttttattaaa
    22861 caaaacgcga aaggtctcgc agcaatctca aatgtagcca aaaaatttag taaaacgttt
    22921 ggcgatgcac gagctaaaga atttacaatc acacacaacc taaaaactcg tgatgttaac
    22981 gtcactatcc gagagactaa cttcccttat gcaatcgtct acgcagatat taaagtcaca
    23041 gacgaaaata gcatcttaat cgcaacaggt gcagaagtat taacgcaaaa tcagtatact
    23101 gtgacaatta ctggatagga ggccgctatg agatatattg gcgaatcaca actctcaaaa
    23161 gaccttgtaa caaaagatta tgtagataac aaagtgacta atctcgcgag taaggatgat
    23221 attgttaatt taggtaaaat taaggactcg caaacaggtg cgtttttgga agtcaaaata
    23281 gtcccaaacg gtcaagtccc ttatgacacc actggcatga ttgtgtttga acgctctgga
    23341 ggtaagtaat gtcagtacaa gagataccag ataaaatttt ttatcgatta atagagcaaa
    23401 cagtagaggc aaagctggtt tgggataagg acacacaaat aggtagctat tatatatcca
    23461 tacacgataa gtatagtttt gaatctggta gagatgttaa catctataaa atcttatcta
    23521 gcacacaggc tgatgtgtat aaagctaata agtataatta tcaatcatca tcaaaaccag
    23581 atactagcgt accacagccg aagccaccag agcaacctcc gctcaaaaac aagtctttgt
    23641 atcaatcaat cacgattgat ggactgccaa tagctcgcgt tatttgtaat aatcaggtca
    23701 tttggtctca atcagcagag cctcaggaag tttacaacat tacagcgtac gtagatgcta
    23761 gcgtatctaa gctaaatgac tctgccatta ttgattacaa tcgagatttt gcggcttacg
    23821 gcgtaaattg gagtcgagtt gatagagtcg ccgttgaagt cgacgataaa atagttgcta
    23881 agctacaagc tcctgtctat gattttggtg ttagaggtgg cacgacgatg tctgtcgaaa
    23941 aatgggatta cccatcaaat acaatagaca aatacaaaaa tccaacattt aaaattttgc
    24001 agcattagga ggtaaagctc ctttagataa gacaaaaccg ctcatcaaat gagcggtttt
    24061 tacattaata aaagaattgc aataaaattt ctaaatccag cagtaggtaa ggttgccaaa
    24121 agcaaaaagt tacttggttg ttaggtagac ttcaatttgg cttgtgtttg agtcaagcgt
    24181 ttcattatct ttatatatca taagatattt agattgagta aattctggtt cagggaaaaa
    24241 atcaaaccaa aaactttctt tattcttagg tatgaacttt atatatccag tttcatattt
    24301 a
//
LOCUS       DAXWIQ010000027        22051 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000027 DAXWIQ010000000
VERSION     DAXWIQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22051
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.027"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            41..229
                     /locus_tag="ACGEUI_001104"
     CDS             41..229
                     /locus_tag="ACGEUI_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HFF8165945.1"
                     /translation="MLYIDEFKEAIDKGYILGDTVAIVRKNGKIFDYVLPHEKVRDDE
                     VVTVERVEEVMVELDYIK"
     gene            complement(381..728)
                     /gene="ssrA"
                     /locus_tag="ACGEUI_001105"
     tmRNA           complement(381..728)
                     /gene="ssrA"
                     /locus_tag="ACGEUI_001105"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            820..1434
                     /locus_tag="ACGEUI_001106"
     CDS             820..1434
                     /locus_tag="ACGEUI_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFF8165946.1"
                     /translation="MTKRNSKAYILWQKIIKILGIIALIGTFFLAFWLYRLGILNDSN
                     ALKDLVQRYRLWGPFVFIVVQIIQIVFPVIPGGLTTVAGFLIFGPVTGFIYNYVGIII
                     GSIALFLLVKTYGRKFILLFVNDKTFYKYERRLETPGYEKLFIFCMASPVSPADIMVM
                     ITGLTDMSLKRFVTILLITKPISIIGYSYLFIFGKDVISWFLQH"
     gene            complement(1561..2346)
                     /locus_tag="ACGEUI_001107"
     CDS             complement(1561..2346)
                     /locus_tag="ACGEUI_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8165947.1"
                     /translation="MFGKLLKYEFRSIGKWYFALNAFVIAIAAILSFTIKLFAQSNSD
                     GLFGVLTNKMLPLTLGLTFGSLIAGSLLSTLLIIIKRFSKSVFGWEGYLTLTLPVNSH
                     QIILSKLLASFICSVFNTIILAFAIAIVIVPMFNINELLEGFFNSFKMDYFINMLTVL
                     AYVLLSTFTSILLIYLSISIGQLFSNRRGLMAFIAYFILVILISVAATYVHSHIFNIN
                     TSADSFPFTEQKTIYLLILEQFIEMIMFYLATNFIIKNKLNLQ"
     gene            complement(2356..3054)
                     /locus_tag="ACGEUI_001108"
     CDS             complement(2356..3054)
                     /locus_tag="ACGEUI_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8165948.1"
                     /translation="MAHLLQLHHVSKSYREKKAIDDLTITIPNGKIIGLLGPNGSGKT
                     TLIKLINGLLQPNKGEIVIDGYRPCVETKKIISYLPDTTYLNENMRIKDMLEFFSDFY
                     SDFDKSKATSLLRDLELDPEDRFKTLSKGNKEKVQLILVMSRKARLYVLDEPIGGVDP
                     AARDYILKTIINSYCENASVIISTHLISDIEPILDEVIFLKQGRLFLSGNADDLRQEY
                     QQSIDSLFRETYKV"
     gene            complement(3054..3425)
                     /locus_tag="ACGEUI_001109"
     CDS             complement(3054..3425)
                     /locus_tag="ACGEUI_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8165949.1"
                     /translation="MSWKFEEKSPIYAQIAQHVMMQIISQEIKSGDQLPTVREYAEIA
                     GVNPNTMQRAFTELEREGMVYSQRTAGRFVTDDQKLIARKRRELAISELESFITNMTK
                     MGFSHTEIIPVLTSFLKEDSQ"
     gene            3610..6720
                     /locus_tag="ACGEUI_001110"
     CDS             3610..6720
                     /locus_tag="ACGEUI_001110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFF8165950.1"
                     /translation="MFAQLDTKTVYSFMDSLIDLNHYFERAKQFGYHTIGIMDKDNLY
                     GAYHFIKGCQKNGLQPVLGLEIEILYQERQVLLNLIAQNTQGYHQLLKISTAKMSGKL
                     HMDYFCQHLEGIAVIIPSKGWSDTLVVPFDYYIGVDQYTDLSHMDSKRQLIPLRTVRY
                     FAQDDMETLHMLHAIRDNLSLAETPVVESDQELADCQQLTAFYQTHCPQALQNLEDLV
                     SGIYYDFDTNLKLPHFNRDKSAKQELQDLTEAGLKEKGLWKEPYQSRLLHELVIISDM
                     GFDDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVAYALNITGIDPVQHDLLFERFLN
                     KERYSMPDIDIDLPDIYRSEFLRYVRNRYGSDHSAQIVTFSTFGPKQAIRDVFKRFGV
                     PEYELTNLTKKIGFKDSLATVYEKSISFRQVINSRTEFQKAFAIAKRIEGNPRQTSIH
                     AAGIVMSDDALTNHIPLKSGDDMMITQYDAHAVEANGLLKMDFLGLRNLTFVQKMQEK
                     VAKDYGCQIDITAIDLEDPQTLALFAKGDTKGIFQFEQNGAINLLKRIKPQRFEEIVA
                     TTSLNRPGASDYTTNFIKRREGQEKIDLIDPVIAPILEPTYGIMLYQEQVMQIAQVYA
                     GFTLGKADLLRRAMSKKNLQEMQKMEEDFIASAKHLGRAEETARGLFKRMEKFAGYGF
                     NRSHAFAYSALAFQLAYFKAHYPAVFYDIMMNYSSSDYITDALESDFQVAQVTINSIP
                     YTDKIEASKIYMGLKNIKGLPRDFAYWIIEQRPFNSVEDFLTRTPEKYQKKVFLEPLI
                     KIGLFDCFEPNRKKILDNLDGLLVFVNELGSLFSDSSFSWVDTKDYSVTEKYSLEQEI
                     VGVGMSKHPLIDIAEKSTQTFTPISQLVKESEAVVLIQIDSIRIIRTKTSGQQMAFLS
                     VNDTKKKLDVTLFPQEYAIYKDQLKEGEFYYLKGRIKERDHRLQMVCQQVQMAISQKY
                     WLLVENHQFDSQISEILGAFPGTTPVVIHYQKNKETIALTKIQVHVTENLKEKLRPFV
                     LKTVFR"
     gene            6800..7813
                     /gene="pfkA"
                     /locus_tag="ACGEUI_001111"
     CDS             6800..7813
                     /gene="pfkA"
                     /locus_tag="ACGEUI_001111"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFF8165951.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGM
                     VDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
                     GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHI
                     IVLAEGVMSGEAFAQKLKEAGDKSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVEL
                     LKDGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGKIIVNNPHKARLDFAALNRS
                     LSQ"
     gene            7876..9378
                     /gene="pyk"
                     /locus_tag="ACGEUI_001112"
     CDS             7876..9378
                     /gene="pyk"
                     /locus_tag="ACGEUI_001112"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFF8165952.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAE
                     LIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIARQKVGFLLDTKGPEMRTELFADD
                     AKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
                     IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEEVREICRETGNDHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAE
                     KPASTDDMFEVAERVAVEAGLVQSGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            9596..10153
                     /gene="lepB"
                     /locus_tag="ACGEUI_001113"
     CDS             9596..10153
                     /gene="lepB"
                     /locus_tag="ACGEUI_001113"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8165953.1"
                     /translation="MVKRDFIRNILLLLIVIIGAILLRIFVFSTFKVSPETANTYLKS
                     GDLVTIKKNIQPKYKDFVVYRVGKKDYVSRVIAVEGDSVTYMDDIFYLNNMVESQAYL
                     EKMKAHYLNHAPFGTLYTDDFTVATITADKYQKVPKGKYLLLNDNRKNTNDSRRFGLI
                     NASQIKGLVTFRVLPLSDFGFVEVE"
     gene            10329..12143
                     /gene="glmS"
                     /locus_tag="ACGEUI_001114"
     CDS             10329..12143
                     /gene="glmS"
                     /locus_tag="ACGEUI_001114"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFF8165954.1"
                     /translation="MCGIVGVVGNRNATDILMQGLEKLEYRGYDSAGIFVANANQTNL
                     IKSVGRIADLRAKIGIDVAGSTGIGHTRWATHGQSTEDNAHPHTSQTGRFVLVHNGVI
                     ENYLHIKTEFLAGHDFKGQTDTEIAVHLIGKFVEEDKLSVLEAFKKSLSIIEGSYAFA
                     LMDSQATDTIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETSEFMEIHDKELVILTKD
                     KVTVTDYDGKELIRDSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRQLISTYADETG
                     NVQVDPAIITSIQEADRLYILAAGTSYHAGFATKNMLEQLTDTPVELGVASEWGYHMP
                     LLSKKPMFILLSQSGETADSRQVLVKANAMGIPSLTVTNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQIAALAFLAKAVGEANGKQEALDFNLVHELSLVAQSIEATLSEKD
                     LAAEKVQALLATTRNAFYIGRGNDYYVAMEAALKLKEISYIQCEGFAAGELKHGTISL
                     IEEDTPVIALISSSQLVASHTRGNIQEVAARGAHVLTVVEEGLDREGDDIIVNKVHPF
                     LAPIAMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            12339..12674
                     /locus_tag="ACGEUI_001115"
     CDS             12339..12674
                     /locus_tag="ACGEUI_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFF8165955.1"
                     /translation="MSLPNCLQCQSEYVYEDGLLLICPMCAFEWTPGEEATEEEGLVV
                     LDSNGVRLSDGDTITVVKDLKVKGAPKDLKQGTRVKNIRLVEGDHNIDCKIDGFGAMK
                     LKSEFVKKI"
     gene            12780..13421
                     /locus_tag="ACGEUI_001116"
     CDS             12780..13421
                     /locus_tag="ACGEUI_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8165956.1"
                     /translation="MTYIQQVLPSLLDGALVTLQVFFIVIILSIPLGAILAFLMKIPF
                     KPLQWFLTLYVWMMRGTPLLLQLIFFYYVLPSVGISFDRMPAAILAFTLNYAAYFAEI
                     FRGGIEAIPKGQYEAAKVLKLKPLQTIRYIILPQVFKIVLPSVFNEVINLVKDSSLVY
                     VLGVGDLLLASKTAANRDATLAPMFIAGLIYLLLIGLVTIISKQVEKRFNYYQ"
     gene            13431..14060
                     /locus_tag="ACGEUI_001117"
     CDS             13431..14060
                     /locus_tag="ACGEUI_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8165957.1"
                     /translation="MLELKNISKQFGQKTIFDGFNLTVQDGEVLSLVGPSGGGKTTLL
                     RMLAGLESIDSGQVFYNGEDVGIDHLENRNLLGFVFQDFQLFPHLTVLDNLTLSPTIT
                     MGKQKADAKEKALDLLARLGLKEHAQVYPYSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQTVEALIVQNREMGITQIVVTHDLVFAEAISDRIIRVNPK"
     gene            14076..14912
                     /locus_tag="ACGEUI_001118"
     CDS             14076..14912
                     /locus_tag="ACGEUI_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8165958.1"
                     /translation="MIIKKRTVAILAIASSFFLVACQATKSLKSGDAWGVYQKQKSIT
                     VGFDNTFVPMGYKDESGRCKGFDIDLAKEVFHQYGLKVNFQAINWDMKEAELNNGKID
                     VIWNGYSITKERQDKVAFTDSYMRNEQIIVVKKRSDIKTISDMKHKVLGAQSASSGYD
                     SLLRTPKLLKDFIKNKDANQYETFTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLEN
                     YRMIPTTFENEAFSVGLRKEDKTLQAKINRAFRVLYQNGKFQAISEKWFGDDVATANI
                     KS"
     gene            15282..16055
                     /gene="cfa"
                     /locus_tag="ACGEUI_001119"
     CDS             15282..16055
                     /gene="cfa"
                     /locus_tag="ACGEUI_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_149017463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfa"
                     /protein_id="HFF8165959.1"
                     /translation="MKPKHLLCLSTVVAGLALFSTMTHSVLADDASNPDTILMNNNQA
                     NFQRDALVQKLDEGHQQLEAIKHEAKGTDIETTVNKAIDAVDHMKSSIRFNTETIYDF
                     SSIGARVEALSDAIKAIVFSTTQLTHKVEKAHTDMGFAITKLVIRIIDPFASVDAIKA
                     QVQEIKALEEKVINYPDLQPTDRATIYTKAKLNKAIWNTRLERDKKVLGIKPFDVYNR
                     LNKAITHAVGVQLNPTTTVQQVDDEVIAVQDALETALKS"
     gene            complement(16424..17659)
                     /locus_tag="ACGEUI_001120"
     CDS             complement(16424..17659)
                     /locus_tag="ACGEUI_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8165960.1"
                     /translation="MTQDPIANLKLARKGPIVSIIVYLSLSVAKLLAGYLLNASSLIA
                     DGFNNLSDIVGNVALLIGLHLASQPADANHKFGHWKIEDLSSLVTSFIMFLVGFQVLI
                     HTIKSIFSGQQVDIDPLGAIVGIVSAFVMLGVYVFNKRLSKRVKSSALVAASKDNLAD
                     AVTSIGTSIAIIAASLHLPVIDHIAAMIITFFILKTAFDIFMESSFSLSDGFDSRHLK
                     KYEKAILEIPKIVAVKSQRARTYGSNVYLDIVLEMNPDLSVYESHSITEKVEQLLSDQ
                     FSIYDIDIHVEPAMIPEEEIFDNVAKKLYRYEKLILSKVPDYDHYIAKSFQLIDANGQ
                     TVNYEQFLNQEIYYPSNFNHFQIESISQKTMLVTYQLNGNQRTSIWRRHESWSLLFHQ
                     ITPIAKKQLHHTHYRIVKM"
     gene            complement(17779..19101)
                     /locus_tag="ACGEUI_001121"
     CDS             complement(17779..19101)
                     /locus_tag="ACGEUI_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFF8165961.1"
                     /translation="MIALVKLIDNLVWGPPLLILLVGTGIYLTSHLGLIQILKLPRAF
                     KLIFSDDEGHGDISSFAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGVLAIKYRTKDANGHISGGPMYYIVNGMGTKWKPLAILFAGSGILVALFGIG
                     TFAQVNSITSSLGHSFGLSPQMVSIVLAIFVAAIIFGGIHSISKVAEKVVPFMAIFYI
                     LSSLAVIFSHYQQLLPVIRLVFQSAFTPTAAIGGFAGSLMKDAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTIIICTLTGLSILVTGQWTGQLEGAPLT
                     QSAFATVFGNLGTFGLTFSLVLFAFTTILGWSYYGERCFEFLFGITHLTYFRIVFILM
                     VGLGGFLKLELIWVLADIVNGLMALPNLIALLALSPVVILETKHYFIK"
     regulatory      complement(19229..19315)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            19630..21948
                     /gene="pcrA"
                     /locus_tag="ACGEUI_001122"
     CDS             19630..21948
                     /gene="pcrA"
                     /locus_tag="ACGEUI_001122"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFF8165962.1"
                     /translation="MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKFVNPWNILAITFTNKAAREMKERALALNPATKDTLIATFHSMCVRILRREADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
                     ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLIKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVL
                     LEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYRANDERDEAVFVAST
                     ISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIA
                     YLTIVANPADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKG
                     KAAQAIMDLANILGQLRQDLDQMSITDLAEALLEKTGYLDSLRLQNTLESQARIENIE
                     EFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEAAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLF
                     GKSSYNRPTRFLKEISEELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQAR
                     KAQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSVEEVTWQIGDIAHHKKWGDGTVLE
                     VSGSGKTMELKIKFPEVGLKKLLASVAPIEKK"
BASE COUNT         7104 a         3746 c         4465 g         6736 t
ORIGIN      
        1 tttttatctt tttacgaata gataggtagg aggatgaaat atgttatata tagatgagtt
       61 taaagaagcg attgataagg ggtatatttt aggtgacaca gtagcgatag tgcgtaaaaa
      121 cggaaagata tttgattatg tgttaccaca cgaaaaagtg agagatgatg aagttgtgac
      181 ggtagagaga gtggaagaag tgatggtgga attagactat atcaaatgaa gaaaactttg
      241 agtagacaga aaaataattg aagagttgtc aatttagttg tcaataatat agaaagttat
      301 ggtaactcat gaacaagaca aaaagaaaaa accttgatgt aacaaggttt tagtaagtta
      361 tgattactta cggtaagcat tgatggagct ggtgggagtc gaacccacgt ccaaacacct
      421 gccaacatat ttgtctacgt ccataggtta tgtcttcatt taacaaacta aagacacata
      481 actcaagcca ttagtttgcg agtctatcaa tctcttgtca cgaaactaga caatttcgta
      541 accgtagctt gctattttga gacttctaac aggacacaag caatcttgta gaagtcacgc
      601 tagctggttt ttaggcagct aaagcgtaag agtttgtatt ttttgcagtt atatttaact
      661 ggcaatttac atctgccgat gggacgcaaa acatgcctca taatgcctgt cgaatccgta
      721 acaaccccaa atagataatt aaccaatttg ttacactaac tattctatca aaactttttt
      781 taaatagcaa aattttacag cttatggtat gattagtcta tgacaaaacg taattcaaaa
      841 gcctatatat tatggcaaaa aattatcaaa attttaggaa ttattgcact aattggtact
      901 tttttcctcg ctttttggct gtatagacta ggtatattaa acgatagtaa tgctcttaaa
      961 gacttagtgc agcgctatag attatggggg ccgtttgttt ttattgtcgt gcagatcata
     1021 cagattgttt ttcctgtgat tcctggaggc cttacgacag ttgcaggttt tttaatcttt
     1081 ggccctgtaa caggttttat ttataattat gtaggcatca ttattggcag tatagccctc
     1141 ttcttgctcg ttaagactta tggtcgtaaa tttatcttac tctttgtaaa tgacaaaaca
     1201 ttttacaagt atgaaaggcg tttggagaca ccaggatatg aaaaattatt tattttttgt
     1261 atggcatcgc ctgtttctcc agctgatatt atggttatga ttacgggatt gacggacatg
     1321 tctttaaagc gctttgtaac tattctttta ataactaaac caatttctat tatcggctac
     1381 agttatttat ttatttttgg aaaagatgtt atatcttggt tcttacagca ttaaggtttg
     1441 gtgttaattt gataaaccgc ttctatttga tggaagacgg tttttttatt tactatgaac
     1501 actgaaaaaa actttctttt aaactatagt gtgtcatagt ttaaaagaaa gtcttaacgc
     1561 ttactgtagg ttgagtttat ttttgataat aaaattagtg gcgaggtaaa acattatcat
     1621 ttcaataaat tgttccaaaa taagaagata aattgttttt tgctcagtaa atgggaaact
     1681 atcggcactt gtattaatat taaagatgtg actgtgaaca tatgttgcag caacgctaat
     1741 cagaataact aatataaaat atgcaataaa ggccatcaag cctcgccgat tggaaaaaag
     1801 ttgacctata gaaatggaaa gataaattaa taagatactc gtaaatgttg ataataggac
     1861 ataggctagt acagttagca tattgatgaa ataatccatc ttaaaactat taaagaatcc
     1921 ttctaatagt tcgttgatgt taaacattgg tacaattaca atagcgatag caaatgcaag
     1981 gatgatagta ttgaaaacac tgcaaataaa agaagctagt agttttgata aaataatttg
     2041 atgcgaattg acgggtaaag tcaacgttaa gtatccttcc catccaaaaa cacttttgct
     2101 gaaacgtttg ataatgataa gtaatgttga taagagggaa ccagcgatca gggagccaaa
     2161 agttaaacct aatgtcagag gcaacatctt attggttagt actccaaata gtccatcact
     2221 attgctttga gcaaacagtt ttatcgtaaa tgacaaaatg gcagcgatgg caataacgaa
     2281 agcattaagc gcaaagtacc attttccaat agatctaaat tcgtatttta ataattttcc
     2341 aaacatgata tcctcctaaa ccttataagt ttccctaaag agggaatcaa tggattgctg
     2401 gtattcttgt cttagatcat cagcattgcc agataagaaa agtcttcctt gtttcaagaa
     2461 aatgacttcg tcaaggatag gttcaatgtc cgaaatcaag tgggttgaga taataacaga
     2521 agcattttca caataactat taatgatggt ttttaggatg tagtctcttg cggcagggtc
     2581 aactccacca ataggttcat caagtacata gagtctggct ttacgactca tcacaagaat
     2641 taattgtact ttttctttgt ttcctttgga tagagttttg aagcggtctt cagggtctag
     2701 ttctaaatct cttagaaggc ttgttgcttt ggatttgtca aagtcgctgt aaaaatcact
     2761 aaagaactcc aacatgtcct taattctcat attttcatta agataagtag tatctggtaa
     2821 ataagaaata attttttttg tctcaacaca gggacggtag ccatcaatga caatttctcc
     2881 tttatttggt tgtaaaaggc cattaatgag tttaattaaa gttgttttac cacttccatt
     2941 tggacctaat aatccgataa ttttaccgtt cgggatagta atggttaagt catcaatagc
     3001 ttttttttca cggtaagatt ttgaaacatg atgtagttgc aataggtgtg ccattattgt
     3061 gaatcctcct ttaaaaagct cgtcaaaaca gggataatct cagtgtgact aaatcccatt
     3121 tttgtcatat tggtgataaa tgattctaat tcactaattg ctaactctcg tcttttacga
     3181 gcaattaatt tttgatcgtc agttacaaaa cggccagcag tacgttgcga ataaaccatt
     3241 ccttcccgtt caagttctgt aaacgctcgt tgcatggtgt taggattaac acctgcgatt
     3301 tctgcatatt cacgaacagt tggaagttga tcaccactct tgatttcttg gctaataatt
     3361 tgcatcatga catgttgtgc tatctgcgca taaataggag atttttcctc aaatttccaa
     3421 gacatgtggt ctcctttctg agtaatatag atatttgtac taattaaatg gtacagaaaa
     3481 aaaaagggaa tgtcaagttt tatgccattt ttcactcttt gagaagtaga cgtagattag
     3541 agagaaaatt gaagcaagaa aacctttttc atagtataat agggaacaag ataaccaaag
     3601 gaggaactta tgtttgctca acttgatact aaaactgtat actcatttat ggatagttta
     3661 attgacttaa atcattattt tgaacgagca aagcaatttg gttaccacac cataggaatc
     3721 atggataagg ataatcttta tggtgcttac cattttatta aaggttgtca aaaaaatgga
     3781 ctgcagccag ttttaggttt ggaaatagag attctctatc aagagcggca ggtgctcctt
     3841 aacttaatcg cccagaatac acaaggctat catcagcttt taaaaatttc cacggcaaaa
     3901 atgtctggca agcttcatat ggattacttc tgccaacatt tggaagggat agcggttatt
     3961 attcctagta agggttggag cgatacatta gtggtccctt ttgactacta tatcggtgtt
     4021 gatcagtata ctgatttatc tcatatggat tctaagaggc agcttatacc cctaaggaca
     4081 gttcgttatt ttgcgcaaga tgatatggaa accctgcaca tgttgcatgc cattcgagat
     4141 aacctcagtc tggcagagac ccctgtggta gaaagtgatc aagagttagc agattgtcaa
     4201 caactaaccg ccttctatca aacacactgc cctcaagctc tacagaattt agaagactta
     4261 gtgtcaggaa tctattatga tttcgataca aatttaaaat tgcctcattt taatagagat
     4321 aagtctgcca agcaagaatt gcaagacttg actgaggctg gtttgaagga aaaaggattg
     4381 tggaaagagc cttatcaatc gcgcttacta catgaattgg tcattatttc tgacatgggc
     4441 tttgatgatt attttttgat tgtgtgggat ttacttcgct ttggacgcag taaaggctat
     4501 tatatgggaa tgggacgtgg ctcggcggca ggtagtctag tggcttatgc tctgaacatt
     4561 acagggattg atccagttca acatgatttg ctatttgagc gctttttaaa caaagaacgt
     4621 tatagcatgc ctgatattga tatcgatctt ccagatattt accgttcaga atttctacgg
     4681 tatgtccgaa atcgttatgg tagcgaccat tcggcgcaaa ttgtgacctt ttcaaccttt
     4741 ggccctaaac aggctattcg tgatgttttc aaacggttcg gggttccaga atacgaactg
     4801 actaatctca ctaaaaaaat tggttttaaa gatagcttgg ctactgtcta tgaaaagtca
     4861 atctctttta ggcaggttat taatagtaga actgaatttc aaaaggcttt tgccattgcc
     4921 aagcgtatcg aaggaaatcc aagacaaacg tccattcacg cagctggtat tgtgatgagt
     4981 gatgatgcct tgaccaatca tattcctcta aaatcgggcg atgacatgat gatcacccag
     5041 tatgatgctc atgcggtcga agctaatggc ctgttaaaaa tggatttttt ggggttaaga
     5101 aatttgacct ttgttcaaaa aatgcaagag aaggttgcta aagactacgg gtgtcagatt
     5161 gatattacag ccattgattt agaagacccg caaacgttgg cactttttgc taaaggggat
     5221 accaagggaa ttttccaatt tgaacaaaat ggtgctatta atcttttaaa acggattaag
     5281 ccacaacgtt ttgaagaaat tgttgccact accagtctaa atagaccagg ggcaagtgac
     5341 tataccacta atttcattaa acgaagagaa ggacaagaaa aaattgattt gattgatcct
     5401 gtgattgctc ccattttaga gccaacttac ggtattatgc tttatcaaga acaagttatg
     5461 cagattgcac aggtttatgc tggttttacg ttaggcaagg ccgacttgtt aaggcgtgcc
     5521 atgtctaaaa aaaatctaca agaaatgcaa aaaatggaag aagactttat tgcttctgct
     5581 aagcacctag ggagagctga agaaacagct agaggacttt ttaaacggat ggaaaaattt
     5641 gcaggttatg gttttaaccg cagccatgcc tttgcctatt cagctttagc ttttcaattg
     5701 gcttatttca aagcccatta cccggctgtt ttttacgata tcatgatgaa ttattctagc
     5761 agtgactata tcacagatgc tctagaatca gattttcaag tagcgcaagt taccattaat
     5821 agtattcctt acactgataa aattgaagct agcaagattt acatggggct gaaaaatatt
     5881 aaggggttgc caagggattt tgcttattgg attatcgagc aaagaccatt taatagcgta
     5941 gaggattttc tcactagaac tccagaaaaa tatcaaaaaa aggttttcct tgagcctctg
     6001 ataaaaatag gtctgtttga ttgctttgag cctaaccgta aaaaaattct ggacaatttg
     6061 gatggtttac tggtatttgt taatgagctt ggttctcttt tttcagattc ttcctttagt
     6121 tgggtagata cgaaagatta ctcagtaact gaaaaatatt ctttggaaca ggagatcgtt
     6181 ggagttggca tgagcaagca tcctttaatt gatattgctg agaaaagtac ccaaactttt
     6241 actcctattt cacagttagt caaagaaagc gaagcagtcg tactgattca aatagatagc
     6301 attaggatca ttagaaccaa aacaagtggg cagcaaatgg cttttttaag tgtgaatgac
     6361 actaagaaaa agctcgatgt cacacttttt ccacaagagt atgccattta taaagaccaa
     6421 ttaaaagaag gagaattcta ttacttaaaa ggtagaataa aagaaagaga ccatcgactg
     6481 cagatggtgt gtcagcaagt gcaaatggct attagtcaaa aatattggtt attagttgaa
     6541 aaccatcagt ttgattccca aatttctgag attttaggtg cctttccagg aacgactcca
     6601 gttgttattc actatcaaaa aaataaggaa acaattgcat taactaagat tcaggttcat
     6661 gtaacagaga atttaaagga aaaacttcgt ccttttgttc tgaaaacggt ttttcgataa
     6721 aaatggacaa ttagtgaagt attttgcaaa tgaatgtgct ataataagac cgttaaatta
     6781 attaaaggag taccagaaaa tgaaacgtat tgctgtttta accagtggcg gcgacgctcc
     6841 tggtatgaac gctgctattc gtgcagtcgt tcgtaaagca atttcagaag gaatggaagt
     6901 ttatggtatc aaccgtggct atgcaggtat ggttgatggc gacattttcc cactgggttc
     6961 aaaagaagtt ggtgataaaa tctcacgtgg tggaactttc ttatactcag ctcgttatcc
     7021 agagtttgct caacttgaag gtcaattggc tggtattgag caacttaaaa aacatggtat
     7081 tgaaggtgtt gttgttattg gtggggatgg ttcttatcat ggcgctatgc gcttgacaga
     7141 gcacggcttc ccagcggtag gtatcccagg aacaatcgat aacgatattg cgggaactga
     7201 ctacactatc ggatttgata cagcagttaa cacagcagtt gaagctattg ataaacttcg
     7261 tgacacctca tcaagtcacg gacgtacttt tgtggttgaa gtgatgggtc gcaatgcggg
     7321 tgatattgct ctttgggcag gtattgctag tggtgctgat caaatcattg ttccagaaga
     7381 agagtttgat attgaaaaag tggcatcaac aatccaatat gattttgaac acaaaggtaa
     7441 aaatcaccac attatcgtct tagctgaagg tgttatgagc ggtgaagctt ttgctcaaaa
     7501 actgaaagaa gctggcgata agagtgacct tcgtgtgaca aatcttggcc acattcttcg
     7561 tgggggttca ccaactgctc gtgaccgtgt cattgcttct tggatgggtt cacatgctgt
     7621 tgagctactt aaagatggca aaggtggttt ggctgttggt attcacaatg aagaacttgt
     7681 tgaaagccca atccttggta ctgcagaaga aggtgctttg ttcagcttga ctgaagaagg
     7741 caagattatt gttaataatc cacacaaagc tcgtttagac tttgcagctt tgaaccgttc
     7801 attgtcacaa taagtttagt taaaattgtt aaatcgtcaa ttattgacaa aaaaataagg
     7861 gagtttcaga gtaaaatgaa taaacgcgta aaaatcgttg caacacttgg tcctgcggtt
     7921 gaaatccgtg gtggtaaaaa atacggagaa gatggctact gggctggtca acttgatgtt
     7981 gaagaatcag caaaaaaaat cgctgaatta attgaagctg gtgcgaatgt tttccgtttc
     8041 aatttctcac acggtgacca caaagagcaa ggtgaccgta tggcaactgt tcgccttgca
     8101 gaagaaattg ctcgtcaaaa agttggtttc cttttggata ctaaaggtcc tgaaatgcgt
     8161 acggaattgt ttgcagatga tgctaaagag tttagctatg ttactggaga aaaaatccgt
     8221 gtagcgacta cacaaggtat tcaatcaact cgcgatgtta ttgctttgaa cgttgctggt
     8281 agccttgaca tctacgatga agtagaagta ggtcatacta ttcttatcga tgatggtaaa
     8341 cttggtttga aagtgatcga caaagatatc gcaacacgtc aatttatcgt tgaagtagaa
     8401 aacgacggta ttattgctaa acaaaaaggt gttaatatcc ctaacactaa aattcctttc
     8461 ccagcacttg cggaacgcga caatgctgat atccgttttg gtcttgaaca aggacttaac
     8521 tttattgcaa tctcatttgt tcgtacagcc aaagacgttg aggaagttcg tgaaatctgt
     8581 cgcgaaactg gtaacgacca tgtgcaatta tttgctaaga ttgaaaacca acaaggtatc
     8641 gataatcttg atgaaatcat cgaagctgct gatggtatca tgattgcacg tggcgacatg
     8701 ggtatcgaag taccatttga aatggttcca gttttccaaa aaatgattat cactaaagtg
     8761 aatgcagctg gtaaagcagt tatcacagca acaaacatgc ttgaaacaat gactgaaaaa
     8821 ccacgtgcga cacgttctga agtatctgac gtcttcaatg ctgttatcga tggtactgat
     8881 gcaacaatgc tttcaggaga gtcagctaac ggtaaatacc cggttgaatc tgttcgtaca
     8941 atggcgacta ttgatcgtaa tgcacaaact cttcttaacg aatatggtcg tttagattca
     9001 tctgccttcc cacgtaccaa taaaacagat gttattgctt cagctgttaa agatgccact
     9061 cattcaatgg acatcaaact tgttgtgact attactgaga caggtaatac agctcgtgcg
     9121 atttctaaat tccgtccaga tgcggacatc ttagctgtaa catttgatga aaaagtacaa
     9181 cgtgctttga tgattaactg gggtgttatt cctgttcttg ctgaaaaacc tgcatctact
     9241 gatgacatgt ttgaagtagc agagcgtgtt gctgtggagg caggcttagt tcaatctggg
     9301 gataatattg ttattgttgc tggtgttcca gttggtactg gtggaacgaa tactatgcgt
     9361 gttcgtacag taaaataaat gtgttataac tatttaccta aaggttagaa ggcaatattc
     9421 taaccttttt tgatatcaaa aatatcatgt taattcacta ctgttaattt actttaatga
     9481 gtctaagttt gtattttata cagaatgagt aacgtttatt ggtattgatc aaaaattgtt
     9541 tacttttttt ctagcttaga acatggtata ataagacaat agactaggag ttaatatggt
     9601 aaagcgagat tttataagaa atatattatt gttattaatt gtgattattg gggctatttt
     9661 gttgagaatc tttgtttttt caacttttaa agtttctcca gaaacagcta atacttattt
     9721 aaagagtggt gatttagtca caatcaaaaa aaatattcag cccaaatata aagattttgt
     9781 ggtttataga gttggaaaaa aagattatgt cagtcgagtc attgctgttg aaggcgatag
     9841 cgtgacttat atggacgata ttttttacct caataatatg gtagagtcac aggcttacct
     9901 tgaaaagatg aaagcacatt acttgaatca tgcaccgttt ggcacattgt atacagatga
     9961 ttttacagtt gctaccatca cagctgataa gtatcaaaaa gttcctaagg ggaagtatct
    10021 tcttttgaat gataaccgga aaaatacgaa tgacagtcgt cgatttgggt taataaatgc
    10081 ctcgcagatt aaaggtttag tgacctttag agttttgcct ctcagcgatt ttggatttgt
    10141 agaagtagag tagttgtcag gctactctac tttttttata attagactat accaatttta
    10201 tgtttgacaa gacattgtgg atattatata ctgtaaacat aatgttttta tcggtataat
    10261 tagactatac caattattga aattatgaga gaaagtagca agttaacgaa cttgttaagg
    10321 taattaagat gtgtggaatt gttggagttg ttggaaatcg caatgcaacg gatattttaa
    10381 tgcaaggcct tgaaaagctt gaataccggg gttatgattc agcaggaatt tttgtggcta
    10441 atgccaatca aacaaacttg attaaatcag tggggcggat tgctgatttg cgtgccaaga
    10501 ttggcattga tgttgctggt tcaacaggga ttggtcacac ccgttgggca acgcatggcc
    10561 aatcaacaga ggataatgcc catcctcaca cgtcacaaac tggacgtttt gtacttgttc
    10621 ataatggtgt gattgaaaat taccttcaca ttaaaacaga gttcctagct ggacatgatt
    10681 ttaaggggca gacagatact gagattgcag tacacttgat tggaaaattt gtggaagaag
    10741 acaagttgtc agtactggaa gcttttaaaa aatctttaag cattattgaa ggttcctacg
    10801 cctttgcatt aatggatagc caagcaactg atactattta tgtggctaaa aacaagtctc
    10861 cattgttgat tggacttggt gaaggttaca acatggtttg ttcagatgcc atggccatga
    10921 ttcgtgaaac cagtgaattt atggaaattc atgataagga gctagttatt ttaaccaaag
    10981 ataaggtaac tgttacagac tacgatggta aagagctgat acgagattcc tacactgctg
    11041 aattagactt atctgatatt ggcaaaggga cttatccttt ctatatgctg aaagaaattg
    11101 atgagcaacc aaccgtaatg cgtcaattaa tttcaactta tgcagatgaa actggtaacg
    11161 tacaggttga tccggctatc attacctcta tccaagaggc tgaccgtctt tatattttag
    11221 cggcagggac ttcctaccat gctggttttg caacaaaaaa tatgcttgag caattgacag
    11281 atacaccagt tgagttgggc gtggcttctg agtggggtta ccacatgcct ctgcttagca
    11341 agaaaccaat gtttattcta ctaagccaat caggagaaac cgcagatagt cgtcaagttt
    11401 tagtaaaggc aaatgctatg ggcattccga gtttgacagt aactaacgtt ccaggatcaa
    11461 ccttatcacg tgaagcaaca tacaccatgt tgattcatgc tggacctgaa attgctgttg
    11521 cgtctacaaa agcttacact gcacaaattg ctgcccttgc ctttttggct aaggcagttg
    11581 gtgaggcaaa tggtaagcaa gaagctcttg actttaactt ggtacatgag ttgtcattgg
    11641 ttgcccaatc tattgaggcg actttgtctg aaaaagatct cgcggcagaa aaggttcaag
    11701 ctttgctagc tactactcgt aatgcttttt acatcgggcg tggcaatgat tattacgttg
    11761 cgatggaagc tgctttgaaa ttaaaagaga tttcttatat tcaatgcgaa ggctttgcgg
    11821 ctggtgaatt gaaacatgga accatttcat taattgagga ggacacgcca gtaatcgctt
    11881 taatatcgtc tagtcagttg gttgcctctc atacgcgtgg taatattcaa gaagttgctg
    11941 cccgtggggc tcatgtttta acagttgtgg aagaagggct tgaccgtgag ggagatgaca
    12001 ttattgtcaa taaggttcat cctttcctag ccccgattgc tatggtcatt ccaactcaac
    12061 tgattgctta ctacgcttca ttacaacgtg gacttgatgt tgataagcca cgtaatttgg
    12121 ctaaagctgt aacagtagaa taaatgatta taatggataa ggcaaccatc tgttaggtgg
    12181 ttgctttttg tttttaggag aatagcagag cttagaaaat ggccttgaga aaagctatac
    12241 aagaagaata gtcatcttct gatgagaacg tattcatctt gtcaaatcat tgattctaaa
    12301 gtaaaatggt aacattaaga aatgtttagg agtgtaagat gtcattacca aattgtttgc
    12361 aatgccagtc tgaatatgtt tacgaagatg gtcttttatt aatctgtcca atgtgtgctt
    12421 ttgaatggac accaggcgaa gaagcgactg aagaagaagg acttgttgtt ttggatagca
    12481 atggtgtccg actgtctgat ggtgatacca ttactgtggt taaagatttg aaagtaaaag
    12541 gcgctccaaa agacctgaaa caagggactc gagttaaaaa tattcgcctg gtggaaggtg
    12601 atcataatat tgattgtaag attgatgggt ttggcgcgat gaaattgaaa tcagaatttg
    12661 tcaagaaaat ctaataataa tacgcaacca gctgatctgg ttgtattttt ataaaaaacc
    12721 tctccccaaa tacgttcaaa tttggtataa tagtgaaata ttcagtttta ggaggcaaca
    12781 tgacctatat tcagcaagtt ttacccagct tattagatgg tgccttggtg actttacaag
    12841 tattctttat tgttatcatt ctttctatac ccttaggggc tattttagct ttcttgatga
    12901 agattccctt taaaccgcta cagtggtttt tgaccctata cgtgtggatg atgcgaggga
    12961 caccattact acttcaattg atttttttct attatgtttt gccaagtgtg gggattagtt
    13021 ttgatcgaat gccagctgct attttggcgt ttactttgaa ttatgctgcc tactttgctg
    13081 aaatttttag aggtgggatt gaggctattc caaaaggtca atatgaagca gctaaagtat
    13141 taaagttaaa acctcttcaa accattcgtt atattatttt gcctcaagtg tttaaaattg
    13201 tgttaccaag tgttttcaat gaagtcatta atttggtcaa agattcttcc cttgtctatg
    13261 tactcggtgt aggagatctt ttattagcaa gtaagacggc agccaatagg gatgcaacct
    13321 tagcccctat gtttatcgct ggtcttatct atttgctttt aattggactg gtcacgatta
    13381 tttcaaaaca agttgaaaaa cggtttaatt attatcagta aggaggcttc atgttagaac
    13441 tcaaaaatat ttccaaacag tttggtcaaa aaaccatttt tgatggcttt aatctaaccg
    13501 ttcaagacgg agaagtgctc tctttggtag gcccatcagg tggcggtaag acaacactct
    13561 tacggatgtt ggctggtctt gaatctattg attcaggtca agtcttttac aatggcgaag
    13621 atgttggtat tgatcatttg gaaaatcgta acttgcttgg atttgttttc caagattttc
    13681 aattgtttcc tcatcttacc gttttagaca acctaacgct gtctccaaca attacaatgg
    13741 gcaagcaaaa ggctgacgct aaagaaaagg ctttggattt actggcacgt ttaggattaa
    13801 aagaacatgc ccaagtttat ccctattctt tatctggagg tcaaaaacaa cgggttgctt
    13861 tggcaagagc catgatgatt gaccctcaaa ttatcggtta cgatgaaccg accagtgctc
    13921 tagatcctga actacgtcag acggtagaag ccttgattgt tcaaaaccgt gaaatgggaa
    13981 taactcaaat tgtagtcact cacgatcttg tttttgccga agccatctca gatcgtatta
    14041 ttagggttaa tcctaaatag aagaggaccc aaaagatgat tataaaaaaa agaaccgtag
    14101 caattttagc catagctagt agctttttct tggtagcttg tcaagctact aaaagtctta
    14161 aatcaggaga tgcttgggga gtttaccaaa agcaaaaaag tattacagtt ggttttgaca
    14221 atacgtttgt tcctatgggc tataaggatg aaagcggcag atgcaaaggt tttgatattg
    14281 atttggctaa agaagttttt caccaatatg gactcaaggt taactttcaa gctattaatt
    14341 gggacatgaa agaagcagaa ctaaacaatg gtaaaattga tgtaatctgg aatggttatt
    14401 caataactaa ggagcgtcag gataaggttg cctttactga ttcttacatg agaaatgaac
    14461 aaattattgt tgtcaaaaaa agatctgata ttaaaacaat atcagatatg aaacataaag
    14521 tgttaggagc acaatcagct tcatcaggtt atgactcctt gttaagaact cctaaactgc
    14581 tgaaagattt tattaaaaat aaagacgcta atcaatatga aacctttaca caagctttta
    14641 ttgatttaaa atcagatcgt atcgatggaa tattgattga caaagtatat gccaattact
    14701 atttagcaaa agaagggcaa ttagagaatt atcggatgat cccaacgacc tttgaaaatg
    14761 aagcattttc ggttggactt agaaaagaag acaaaacgtt gcaagcaaaa attaatcgtg
    14821 ctttcagggt gctttatcaa aatggcaaat ttcaagctat ttctgagaaa tggtttggag
    14881 atgatgttgc cactgccaat attaaatctt aaaaaggaca cttgtccttt ttttgtttaa
    14941 caggtgagat ctagcagaca aagtcagcta gacgatttaa taaaaatctt agtttaaggt
    15001 tttttattaa actgtatttt ttctgaaaat tatagttttt ttaaaactga taaatgacaa
    15061 gagaagttat tttaggtcaa ttaacgtcca tttaaaagta atactaggca tttaatgaca
    15121 ttaaaaaatg attatttttg cgaaattctc ttattttctg gaaattaaag aaatattgac
    15181 cttagtaaaa acaagtagta tgataacgtt ataagcgctt ataaattaat ttaacttcac
    15241 ttaactattt aaatagattt attatattca ggaggaataa tatgaaaccc aaacaccttc
    15301 tctgtctttc aacagttgtt gctggattag ctttattttc aactatgacg cactcagttt
    15361 tagctgatga tgcttcaaat cccgatacta ttttaatgaa taacaaccaa gcaaattttc
    15421 aaagagatgc tctagtgcaa aagcttgatg agggacatca acaattagaa gctattaaac
    15481 atgaagctaa aggtactgat attgagacta ctgttaacaa agctatcgat gctgttgatc
    15541 acatgaagag ttctatacgc ttcaatactg aaacgatcta tgattttagt tcaattgggg
    15601 caagagtaga agcattatca gatgctatca aagcaatcgt attttccaca acccaattaa
    15661 ctcataaagt agaaaaagcg catactgata tggggtttgc tattactaaa ttagtcattc
    15721 gtattataga tccatttgca tctgttgacg ctatcaaagc tcaagtacaa gaaattaaag
    15781 cacttgagga aaaagttatc aattatcctg atttacagcc aacagaccgt gctactatct
    15841 atactaaagc taaactaaat aaagctattt ggaacactcg tttagaaaga gataaaaagg
    15901 ttttgggaat caaaccattt gacgtttaca atagacttaa caaagctatc acacatgcgg
    15961 ttggtgtcca actaaatccg acaaccactg ttcaacaagt tgacgatgaa gttattgctg
    16021 tgcaagatgc tttggaaaca gctcttaagt catagagtgt ataagtaatg tgttgtatag
    16081 gtagatgatt acattaggat atagaatagg taaagatttt aaatatctta ccattgtaga
    16141 gtttgaggtg atgttctcat aaacaatgag tcacctgtag ccctttttgc ctttaacatg
    16201 atgtaccaat ttaaggtatt gttcaaccgt cactattgaa tcttgtttaa ccccctaaac
    16261 taataagatt caatatgaaa acgtgtattc ttaaaaaaat ccctatgatc tgtttaaaaa
    16321 caaattgtag ggatttttta actatttcac ttatcatttt gggactagga atcgctttag
    16381 gaatatcatg aagtatagtt atgttataag agaagtgatg tttttacatt tttacaatac
    16441 gatagtgtgt gtgatgtaat tgtttcttag cgataggggt aatttggtgg aagagtaagg
    16501 accaagattc atgacgcctc caaatactgg tacgttgatt gccatttaat tggtaagtta
    16561 ccaacatcgt tttttgacta atggattcaa tctgaaaatg gttgaagtta cttggataat
    16621 aaatttcttg gttcaaaaat tgttcatagt taactgtttg gccattcgca tcaatcagtt
    16681 ggaaagactt agcaatgtag tggtcatagt caggaacctt actcaaaatt aatttttcgt
    16741 agcggtagag ctttttggcg acattatcaa aaatctcttc ttcgggaatc atggcaggct
    16801 caacgtgaat gtcaatgtca taaatagaaa attggtcact caataactgc tccactttct
    16861 ctgtaataga gtgactttca tagactgaaa gatcaggatt catttcaagt acaatatcaa
    16921 gatagacatt gctaccatag gtcctagctc gttgagactt aacggcgaca attttaggga
    16981 tttctaaaat ggctttttcg tattttttca aatgacggct atcaaatcca tcagataaac
    17041 taaacgaact ttccataaag atatcaaaag ctgttttaag aataaagaac gtaatgatca
    17101 tagcagctat atgatcgata actggtaaat gcaaagaagc tgctataata gcaattgatg
    17161 ttccgataga agtaacagca tcagctagat tatccttaga agcagcgact aaggcactgg
    17221 attttacacg tttggaaaga cgtttgttaa agacataaac ccctaacata acaaaagctg
    17281 aaacgatacc gacaatagcc ccaagagggt caatatcaac ttgctgacca ctaaagatac
    17341 ttttaattgt gtgaatcagt acttggaaac ctacaagaaa cataataaaa gaagtgacaa
    17401 ggctggataa gtcttcaatt ttccaatgac caaatttatg attggcatcg gctggttggc
    17461 tagctaagtg aagaccaata agcagggcta catttcccac aatatccgat aaattgttaa
    17521 atccatcagc aataagggaa cttgcattta acaaataacc agctaataat ttggcaacac
    17581 taagcgacaa gtagacaata atactaacga ttgggccctt tctggctagt tttaaatttg
    17641 caatcggatc ctgtgtcata ataactcctt tgatttcaac tttatattat accataaaaa
    17701 ggggcttaag aaaaaagcct agcgtcttga acttggcaga agaagactag gtataactgg
    17761 gtagtactaa cgtcatgttt acttaataaa gtaatgcttg gtttctaaaa taacaactgg
    17821 cgagagagct aggagggcaa tcaggttagg aagtgccatt aaaccattga caatatcagc
    17881 caaaacccaa attagttcta acttaaggaa gcctcctagg cctaccatta agataaagac
    17941 aatacggaag taggtgagat gtgtaatgcc aaagaggaat tcaaagcagc gttctccata
    18001 ataactccat cctaaaatcg ttgtaaaggc gaagagaact agggaaaagg ttaggccaaa
    18061 agtccctaaa tttccaaaca cagtggcaaa ggcagattgg gtcagaggag ccccttcgag
    18121 ctgcccagtc cattggcctg ttactaaaat agataggccg gttagggtac agataataat
    18181 cgtatcaata aaagttcctg tcatggagat cagtccctgc tccacgggtt cattggtttt
    18241 agctgctgct gcagcaatag gtgcagatcc tagtccagac tcattggaga agacaccgcg
    18301 tgcaattcct ttttgaatgg catccttcat gagacttcca gcaaagcctc caatagcagc
    18361 tgtcggagtg aaggctgatt gaaagactaa acgaataaca ggaagtagct gttggtagtg
    18421 actaaaaata actgctaaac tagatagaat ataaaaaata gccataaaag gaacgacttt
    18481 ttcagccact ttggaaatgg aatgaatgcc accaaaaata atggcagcca caaagatagc
    18541 taaaacaata ctgaccattt gtggggataa gccaaagcta tgacctaaag acgaggtgat
    18601 cgagttcact tgagcaaagg ttcctattcc aaatagggca actaggatcc ctgaacccgc
    18661 aaaaagaatg gctaagggtt tccattttgt ccccatgcca ttaacaatat agtacatagg
    18721 accaccagaa atatgaccgt tggcatcctt tgtacggtat ttaatcgcta gtaccccttc
    18781 agcgtactta gtggccattc caaaaaaagc ggcaacccac atccaaaaga gcgctccagg
    18841 accaccagac ttgatagcag tggcaacccc aacaatgtta ccagttccga cagtagcggc
    18901 aagggcagtt gcaagagcag caaaggatga aatatctcca tgtccttcgt catctgaaaa
    18961 aatgagttta aaggctcttg gtagttttaa gatttgaatt aatcctaaat gactggtaag
    19021 gtaaatcccc gtcccaacca ataaaattaa gaggggcggt ccccaaacaa ggttatcaat
    19081 taatttaacg agtgctatca tctttctctc ctaaaagtca tcgaagacgc caaaagaaaa
    19141 aagctatctc tttctttgag acaacttctg aagcaatgat aaaacagcaa acacaaaaaa
    19201 gactaagact gttcttaatg gtgtttgtat tttaccttct gtccttttgc ctgagagatt
    19261 gagcagtatg ccttgcccct tcggcgccta ataaggtctc tccagaagtc cgtccatcaa
    19321 acagtcccgt tcaaaaactg aacacctgag agtataactc cttcggcgaa aaaaattctt
    19381 ctcctgcatg acatcattcg gtttattaaa ttaggactat catacctcaa aaaacgaatg
    19441 aagtcaacca aaatagcaaa aaaataagga atgctttaag aaatcgctat tatttttaaa
    19501 tatctgatcc taaactagaa aaaaggaatc tctttacttt tggggtcttc tttgctattt
    19561 ttcttggaaa catgatataa ttttaaggtt gataaaagag atgtcactga acgagattaa
    19621 ggagaaagca tgaatccttt attaaatgga atgaatgatc ggcaggcaca agctgttcaa
    19681 acaactgaag gccccttatt gattatggcg ggggctggtt ctggtaaaac aagggtactg
    19741 acccatcgta ttgcctattt aattgatgaa aaatttgtca atccttggaa tattttagct
    19801 attaccttta ctaataaggc tgcgcgtgaa atgaaagaaa gggccttggc cttaaatcca
    19861 gctactaaag ataccttaat tgctaccttt cactccatgt gtgtacggat tctgcgtcgt
    19921 gaggctgatc acattggtta caaccgaaac ttcacaatcg tggatccagg tgagcaacga
    19981 acgttgatga agcgcatttt aaaacaatta aatatagacc ctaaaaagtg gaatgaacgt
    20041 tctattttag gaactatttc aaacgctaaa aatgatttgc ttgatgaaaa gggatacgag
    20101 gcacaggctg ctgatatgta tagtcaaatc gtagcgaggt gttacaaagc ttatcaggaa
    20161 gaattacgac gtagcgaagc acttgacttt gatgatttaa tcatgatgac gctacgcctg
    20221 tttgacgcta atcctgacgt gttagcttat taccagcagc gttaccaata cattcatgtg
    20281 gatgagtacc aagataccaa ccatgctcaa tatcagctaa ttaaattact agcttctcgc
    20341 tttaaaaata tttgcgttgt tggagacgct gatcagtcta tctatggttg gcggggagct
    20401 gacatgcaaa atattcttga ttttgaaaaa gattacccag atgctaaggt agtgctttta
    20461 gaagaaaact atcgctctac caagaaaatt ctgcaagcag ctaacgacgt gattaacaat
    20521 aaccgtaatc gccgtcctaa gaaactttgg acacaaaatg cggatggtga acaactggtt
    20581 tattaccggg caaatgatga gcgagatgag gcagtatttg tggcctcaac cattagcaat
    20641 atgagtcaag agcttggaaa aaacttcaaa gatttcgctg ttttataccg taccaatgcc
    20701 caatctcgga ccattgaaga agcactctta aaatccaata ttccttacac catggttgga
    20761 ggcaccaagt tttacagccg taaagaaatt cgggatttga ttgcctattt aaccattgtt
    20821 gctaaccctg ctgataatat ttcttttgag cggattgtga atgagcctaa acgaggtgtt
    20881 ggtccaggaa cgcttgataa actgcgtcag tttgcctatg agagtgacca gtctttatta
    20941 gaagcagctt caaatctatt gatgtccccc ttgaaaggta aagcagcaca agctatcatg
    21001 gacttggcca atattctcgg tcagctacgt caagacctag atcagatgag tatcaccgac
    21061 ttagcagaag ctttgttaga aaaaacaggt tatttagata gcttacgcct gcaaaataca
    21121 ttggaaagcc aagcccgtat cgaaaatatc gaagaattct tatctgttac caaaaatttt
    21181 gacgagtcaa gtgcctctca agaagaagac gaaaccggtg ttgatcgctt gggacgtttt
    21241 ttaaacgatt tagcactgat tgctgataca gatgattccc aagcagaagc tgctgaagta
    21301 accttaatga ccctacatgc tgccaaaggc ttggagttcc ctgttgtttt cttaattggt
    21361 atggaagaag gtgtcttccc cttatcacgt gcctctgaag atccagatga attggaagaa
    21421 gaaagacgtt tagcttatgt cggaatcaca agagctgaag aagttctctt tatgaccaat
    21481 gccaacacca gaaccctctt tggtaaaagt agttataacc gtccaacacg cttcttgaaa
    21541 gaaatttcag aggaactact ctcttataaa ggattggcaa gaccagcaca atcttcattt
    21601 ggtgtgcgtt tttctactga aacacacaca cagtttggac aagggatgag cctatcagaa
    21661 gccttacaag ctcgaaaggc ccaggctcaa gtaagacaat cagcacagcc aatgcaggca
    21721 catacgatac catcagcttc aactagctca gttttaccgt ttggttctaa tagctctgta
    21781 gaagaggtca cttggcagat tggcgatatc gctcaccata aaaaatgggg agacgggact
    21841 gtgcttgagg tttcaggctc cggaaaaacg atggaattga aaatcaaatt tccagaagtt
    21901 ggtttaaaga aattactagc aagtgttgct cctattgaga aaaaataaga aaaaaatgag
    21961 cttcctacta tataagtgag taggaagctc atttttttta gatttctgta tcatataaaa
    22021 gttaaaaaat tttaagtttt aggagcatat c
//
LOCUS       DAXWIQ010000028        21716 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000028 DAXWIQ010000000
VERSION     DAXWIQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21716
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.028"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            79..444
                     /locus_tag="ACGEUI_001123"
     CDS             79..444
                     /locus_tag="ACGEUI_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8165963.1"
                     /translation="MTVKINTKDGLIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQSLLRKENYAKGVVVKSTDLGISVDVYTVMSYGVKISEVSKNIQERVKFNLESQLG
                     LTADMVNVYVQNIKVVGEN"
     gene            444..2108
                     /locus_tag="ACGEUI_001124"
     CDS             444..2108
                     /locus_tag="ACGEUI_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFF8165964.1"
                     /translation="MSNITTSLFQEMVQAAATRLGKQAEYVNSLNVFPVPDGDTGTNM
                     SMTMDNGAKEVADKPASTVGEVGQMLSKGLLMGARGNSGVITSQLFRGFGQSIKGKDE
                     LTGKDLAQAFQVGVEVAYKAVMKPVEGTILTVSRGAATAALKKADLTDDAVEVMQAAL
                     DGAKGALAKTPDLLPVLKEVGVVDSGGQGLVFIYEGFLSALNGDYVTSADFKATPANM
                     SEMINAEHHKSVVGHVATEDITYGYCTEIMVALKQGPTYVKEFNYDEFQGYLSGLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLIKIKVDNMRNQHEAQVQKTDVEKNKA
                     EVKDFGLIAVVAGEGLSEIFKAQGVDYVISGGQTMNPSTEDIVKAIEAVNAKQVIILP
                     NNKNIFMAAQSAAEVVDIPAAVVATRTVPQGFTSLLAFDPSKSLEDNVADMSTSLSDV
                     VSGSVTLAVRDTTIDGLEIHENDFLGMVDGKIIVSNPDMEATLKAAFEKMIDEDSEIV
                     TIFVGEEGDQDLAEELAGYLGETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            2345..3235
                     /locus_tag="ACGEUI_001125"
     CDS             2345..3235
                     /locus_tag="ACGEUI_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFF8165965.1"
                     /translation="MLGPFIFIAFGVIVILAIVASTLYVVRQQSVAIVERFGRYQKTA
                     TSGIHIRLPFGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNVATQYRVNEQNVTDA
                     YYKLMKPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIV
                     KTLITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVG
                     IAQQRKAIVDGLAESIQELKEANISLNEEQIMSILLTNQYLDTLNTFAAKGNQTLFLP
                     NTPSGVEDIRTQVLSALKTK"
     gene            3465..4319
                     /locus_tag="ACGEUI_001126"
     CDS             3465..4319
                     /locus_tag="ACGEUI_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HFF8165966.1"
                     /translation="MKSKKVVSVISLTLSLFLVTGCAKVDNNKSVNLKPATKQTYNSY
                     SDDQLRSRENTMSVLWYQRAAETQALYLQGYQLATDRLKEQLNKPTDKPYSIVLDIDE
                     TVLDNSPYQAKNVLEGTGFTPESWDYWVQKKEAKPVAGAKDFLQFADQNGVQIYYISD
                     RSTTQVDATMENLQKEGIPVQGRDHLLFLEKGVKSKESRRQKVKETTNVTMLFGDNLL
                     DFADFSKKSQEDRTALLSDLQEEFGRRFIIFPNPMYGSWEGAIYKGEKLDVLKQLEER
                     RKSLKSFK"
     gene            4512..5708
                     /gene="pgk"
                     /locus_tag="ACGEUI_001127"
     CDS             4512..5708
                     /gene="pgk"
                     /locus_tag="ACGEUI_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFF8165967.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGSKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAEFDQALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            5971..6522
                     /locus_tag="ACGEUI_001128"
     CDS             5971..6522
                     /locus_tag="ACGEUI_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFF8165968.1"
                     /translation="MKTLRKLLSNYKFDIKKFKLGMRTLKTGLSVFLVLLVFHLFGWK
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMVNVACNNKSGIIGAVAALLIITLSIPTGQTFIYVTSRVFETFCGVFVA
                     ILVNTDVELIKNKWFNKKHSDVR"
     gene            6589..6960
                     /locus_tag="ACGEUI_001129"
     CDS             6589..6960
                     /locus_tag="ACGEUI_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8165969.1"
                     /translation="MKEKELRRSMAVFPIGTVMTLTDLSARQIRYYEDQGLIKPERTQ
                     GNRRMFSLNDMDRLLEIKDFLSEGLNIAAIKREYVERQGKLMQKQKALTDADVRRILH
                     DEMLTQSGFSTPSQHIGNFRI"
     gene            6998..8344
                     /gene="glnA"
                     /locus_tag="ACGEUI_001130"
     CDS             6998..8344
                     /gene="glnA"
                     /locus_tag="ACGEUI_001130"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFF8165970.1"
                     /translation="MAITVADIRREVKEKNVTFLRLMFTDIMGVMKNVEIPATKEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     KPFAGDPRGNLKRALKHMNEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDL
                     APIDLADNTRREIVNILTKMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLV
                     VKTIAREHGLYATFMAKPKFGIAGSGMHCNMSLFDNQGNNAFYDEADKRGMQLSEDAY
                     YFLGGLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALAVLLEAGLDGIINKIEAPEPVEANIYTMTMEERNEAGIIDL
                     PSTLHNALKALQKDDVVQKALGYHIYTNFLEAKRIEWSSYATFVSQWEIDHYIHNY"
     gene            complement(8571..10253)
                     /gene="rnjA"
                     /locus_tag="ACGEUI_001131"
     CDS             complement(8571..10253)
                     /gene="rnjA"
                     /locus_tag="ACGEUI_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J1"
                     /protein_id="HFF8165971.1"
                     /translation="MTNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVDNLDRVKALVITHGHEDHIGGIPFLLKQANIPIYAGPLA
                     LALIRGKLEEHGLWREATVYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGK
                     IICTGDFKFDFTPVGDPADLQRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSIL
                     KIIEGIHGRIIFASFASNIYRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVH
                     GEYRMQKVHAGLAMDIGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRRDLSEDGVVLAVATVDFNTQMILAGPDILSRGFIYMRESGDLIRESQR
                     VLFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDKH"
     gene            complement(10255..10485)
                     /locus_tag="ACGEUI_001132"
     CDS             complement(10255..10485)
                     /locus_tag="ACGEUI_001132"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase subunit epsilon"
                     /protein_id="HFF8165972.1"
                     /translation="MIYKVFYQETKDQSPRRESTKALYLNIDATDELDGRIKARRLVE
                     DNTYYNVEFIELLSDKHLDYEKETGVFELTEF"
     gene            10770..11468
                     /gene="tsaB"
                     /locus_tag="ACGEUI_001133"
     CDS             10770..11468
                     /gene="tsaB"
                     /locus_tag="ACGEUI_001133"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFF8165973.1"
                     /translation="MKTLAFDTSNKTLSLAILDDETLLADMTLNIQKNHSVSLMPAID
                     FLMTCTDLKPQDLERIVVAKGPGSYTGLRVAVATAKTLAYSLNIALVGISSLYALAAS
                     TCKQYPNTLVVPLIDARRQNAYVGYYRQGKSVMPQAHASLEVIIEQLVEEGQMIFVGE
                     TAPFAEKIQKKLPQAILLPTLPSAYECGLLGQSLAPENVDAFVPQYLKRVEAEENWLK
                     DNEIKDDSHYVKRI"
     gene            11470..11895
                     /gene="rimI"
                     /locus_tag="ACGEUI_001134"
     CDS             11470..11895
                     /gene="rimI"
                     /locus_tag="ACGEUI_001134"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFF8165974.1"
                     /translation="MKTVEEQAKNIYQLLEMVYGTSPWTLEQVLIDIRRDQTDYFLLY
                     DHDKLLGFLAIQDLAGEVEMTQIAILPSHQELGLASQLMTHLDSIESDIFLEVRESNH
                     RAQGLYQKFGFKFIGKRPDYYRNPIETALLMKREGKHDR"
     gene            11885..12913
                     /gene="tsaD"
                     /locus_tag="ACGEUI_001135"
     CDS             11885..12913
                     /gene="tsaD"
                     /locus_tag="ACGEUI_001135"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFF8165975.1"
                     /translation="MTDRYILAVESSCDETSVAILKNESTLLSNVIASQVESHKRFGG
                     VVPEVASRHHVEVITTCFEDALQEAGISASDLSAVAVTYGPGLVGALLVGLAAAKAFA
                     WANHLPLIPVNHMAGHLMAAREQKPLVYPLIALLVSGGHTELVYVPEPGDYHIIGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMITEDHLEFSFSGLKSA
                     FINLHHNAKQKGDELILEDLCASFQAAVLDILLAKTKKALSRYPAKMLVVAGGVAANQ
                     GLRDRLAQEITHIEVVIPKLRLCGDNAGMIALAAAIEYDKQHFANMSLNAKPSLAFDQ
                     FPDSFVIN"
     gene            complement(13084..13353)
                     /gene="rpsN"
                     /locus_tag="ACGEUI_001136"
     CDS             complement(13084..13353)
                     /gene="rpsN"
                     /locus_tag="ACGEUI_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFF8165976.1"
                     /translation="MAKKSKIAKYQKQLQLIEQYADLRRDLKAKGDYESLRKLPRDSN
                     PNRLKNRDKIDGRPHAYMRKFGVSRINFRDLAHKGQLPGVTKASW"
     gene            complement(13589..14332)
                     /locus_tag="ACGEUI_001137"
     CDS             complement(13589..14332)
                     /locus_tag="ACGEUI_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8165977.1"
                     /translation="MSTNDLTKKLKKLKHVQVYNTIFQLIQDGTYSPGMQLPSEPELA
                     RQLNVSRMTLRKSLALLQEDHLIKNIRGKGNFILKTPETKYHQGFEYLQHPIYASLSS
                     DITKVELEYRIEVPTVAITASLKQETPVVIIVDRWYHSQNKAIAYSLSFIPIEVISKY
                     AINLNQEEPLLTFLEEKIYESGKASHSCNQIGYTKTGNYTATKYTLSENSAFILIQET
                     LYNGKDILVSTKHYVPADLFDLKVQSQSC"
     gene            14572..15351
                     /gene="udp"
                     /locus_tag="ACGEUI_001138"
     CDS             14572..15351
                     /gene="udp"
                     /locus_tag="ACGEUI_001138"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFF8165978.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            15372..16574
                     /locus_tag="ACGEUI_001139"
     CDS             15372..16574
                     /locus_tag="ACGEUI_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFF8165979.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQIVSVVSTGVTSVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILLPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRSTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            16604..17275
                     /gene="deoC"
                     /locus_tag="ACGEUI_001140"
     CDS             16604..17275
                     /gene="deoC"
                     /locus_tag="ACGEUI_001140"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFF8165980.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIQNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAKCQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAKTFIALGASRLGTSRIIKIVKNEAT
                     KTDSY"
     gene            complement(17370..18539)
                     /locus_tag="ACGEUI_001141"
     CDS             complement(17370..18539)
                     /locus_tag="ACGEUI_001141"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8165981.1"
                     /translation="MTQYDTIIIGGGPAGMMAAISSSYYGYKTLLIEKNRRLGKKLAG
                     TGGGRCNVTNSGNLDVLMAGIPGNGRFLYSVFSQFDNHDIIAFFEENGVKLKEEDHGR
                     MFPTTDKSRTIIDALEKKIKALGGQVLTSTEVVSVKKQDDLFYLKSADQTFTCQKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLTVTDLEAAESPLLTDFPHKVLQGISLDDVTLSY
                     DKHVITHDLLFTHFGLSGPAALRLSSFVKGGEIAELDFLPHLSTDDLTAYLSDQRDKN
                     IKNALKGLLPERVADFLSEDYPEKVKQLSPKQEKELLDKLKHLQIPITGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVPGLYFAGEVLDINAHTGGFNITSALCSGWIAGKSS"
     gene            18745..19275
                     /locus_tag="ACGEUI_001142"
     CDS             18745..19275
                     /locus_tag="ACGEUI_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014634931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFF8165982.1"
                     /translation="MGIEKTVSELADILGVSRQAVNNRVKSLPEEDLDKNEKGVTVVK
                     RSGLVKLEEIYKKTIFDDEPISEETKQRELLEILVDEKNTEITRLYEQLKAKDAQLAS
                     KDEQMRVKDVQIAEKDKQLDQQQQLTAKAMADKETLKLELEEAKAEANQARLQVEEVQ
                     AEVGPKKGFFTRLFAK"
     gene            19324..19911
                     /locus_tag="ACGEUI_001143"
     CDS             19324..19911
                     /locus_tag="ACGEUI_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFF8165983.1"
                     /translation="MKHLDTYIAFDLEFNTVNDVSHIIQVSAVKYDHHKEVDSFDTYV
                     YTDVPLQSFINGLTGITSDKIAAEPKVEEVMAAFKNFVGELPLIGYNAQKSDLPILAE
                     NGLDLRDQYQIDLFDEAYDRRSADLNGIANLRLQTVATFLGIKGRGHNSLEDARMTAV
                     IYKSFLETDTNKAYLSQQEEVTTDNPFAALGDFFD"
     gene            20060..20797
                     /locus_tag="ACGEUI_001144"
     CDS             20060..20797
                     /locus_tag="ACGEUI_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8165984.1"
                     /translation="MSQFYSTGELANLAGVSIRTVQYYDQRGILIPTALTAGGRRLYT
                     DSDLEQLRMICFLRDLGFSIEQIRKVLAEENAAQVLELLLVDHIATAKEDLAAKEQQV
                     DIAVKILDRLRKQDPQSLDFLMDISLSMKNQKAWKKLQWKMGISIFLAIVSYVGLILL
                     SSYLQKFWLVYVAMGLFLPGFSWLVIYFRKQFIYLCPHCHQKFEPSFNEFALAGHTPR
                     TRKLTCSHCHQKSYCLELAKEDTVSRR"
     gene            complement(20854..21153)
                     /locus_tag="ACGEUI_001145"
     CDS             complement(20854..21153)
                     /locus_tag="ACGEUI_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFF8165985.1"
                     /translation="MMNMQNMMKQAQKLQKQMEQKQADLAAMQFTGKSAQDLVTATFT
                     GDKKLVGIDFKEAVVDPEDVETLQDMTTQAINDALTQIDETTKKTLGAFAGKLPF"
     gene            complement(21346..>21716)
                     /locus_tag="ACGEUI_001146"
     CDS             complement(21346..>21716)
                     /locus_tag="ACGEUI_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8165986.1"
                     /translation="AKKTFFASNLKYLRLKKNMEQLELANLLGRKSSSSISEWEKGKY
                     TPKSGLLSDIAAIFDVSLTALMEEDLTLENKGVSYQLPQRELAMISEQLSENNYTKWV
                     DFAQILLEQQAKEDRELDNH"
BASE COUNT         6778 a         3685 c         4547 g         6706 t
ORIGIN      
        1 gttagctatt ctatctcttt tacattactt ctaaaatatg ataaaatagg atgataaaaa
       61 ctttttgagg tagtaactat gactgtaaaa attaatacaa aagatggtct aatcgagcta
      121 tccgacgatg tgattgcaac tgtcgtggga ggatcagcaa cggaaatttt tggtgttgtt
      181 ggaatggcca gcaaaagtgc aataaaagac aattttcaat cactacttcg caaagaaaac
      241 tacgctaagg gtgttgtagt aaaatcaaca gatctgggta tttctgttga tgtctacact
      301 gtgatgagtt atggtgtgaa aatcagtgaa gtctctaaaa atatccaaga acgcgtgaaa
      361 tttaaccttg aaagtcagct tggtctcact gctgatatgg ttaatgttta cgtgcaaaat
      421 attaaggttg taggagaaaa ttagtgtcaa atattacaac aagcttattc caagaaatgg
      481 tccaagcggc agcgactcgc cttggcaaac aagcagaata tgttaattcc ttaaatgttt
      541 ttcctgttcc agatggtgat acaggaacaa acatgagcat gactatggac aatggtgcca
      601 aggaagttgc tgataaacct gcgtcaactg tgggagaagt tggacaaatg ttatcaaaag
      661 gtcttttgat gggagcgcgt ggaaattctg gagttattac ttcacaactt tttcgtggtt
      721 ttggtcaaag cattaaggga aaagatgagc tgacaggtaa agatttggca caagcctttc
      781 aagtgggtgt agaagttgcc tacaaagcag tgatgaaacc tgttgaagga acaattttaa
      841 ctgtttctcg gggtgcagcg actgctgcct taaaaaaagc agatcttaca gacgacgccg
      901 tggaagtcat gcaagctgct ttagacggtg ccaaaggtgc ccttgctaag acacctgatt
      961 tattaccagt ccttaaagaa gttggtgtgg ttgattcagg cggtcaaggt cttgtcttta
     1021 tctatgaagg tttcctatct gctttgaatg gcgactatgt gacttcagca gactttaaag
     1081 cgactcctgc taacatgtct gaaatgatta atgctgagca tcacaaatcc gttgttggtc
     1141 atgtggctac tgaagacatt acctacggtt attgtactga aatcatggtt gcccttaaac
     1201 aaggcccaac ctatgtgaaa gaattcaact atgatgagtt tcaaggctac cttagtggcc
     1261 ttggtgactc gctgttagtg gtcaatgatg atgagattgt taaagttcac gtccatactg
     1321 aagaccccgg acttgtgatg caagaaggtc ttaaatatgg ttcgctcatc aagattaaag
     1381 tggacaatat gcgtaaccag catgaagcac aggttcaaaa gactgatgtc gaaaaaaaca
     1441 aggctgaagt aaaagacttt ggcttgattg cagtagtagc tggagaaggt ttatctgaaa
     1501 ttttcaaggc gcaaggtgtt gattacgtga tttctggtgg tcaaaccatg aatccatcta
     1561 cagaagatat tgttaaagct attgaagcag ttaatgccaa acaagtcatt atcttgccaa
     1621 acaataaaaa tatctttatg gctgcccaat ctgctgcaga agtggtggat attccggctg
     1681 ctgttgttgc aacacgtact gttccgcaag gatttaccag cttgttggcc tttgatcctt
     1741 caaaatcttt agaggacaat gtagcagata tgtcaactag tctttctgac gtggtcagtg
     1801 gaagcgttac tttggcagtc cgtgacacca ccattgatgg tttagaaatc catgaaaatg
     1861 atttcttggg aatggttgat ggtaaaatca tcgtttcaaa cccagacatg gaagcaactc
     1921 taaaagctgc ctttgaaaag atgattgacg aagatagcga gattgtgact atctttgttg
     1981 gtgaagaagg tgatcaagac ttagcagaag aacttgctgg gtatttagga gagacttacg
     2041 aagatgtgga agttgagatc catcaaggag atcagcctgt ttacccatat ctcatgagtg
     2101 tagaataata gaatataata aaaaaagagg agttaggcaa aggtctgact ctttttgtct
     2161 atattgatgt cgtaatgagt ggttttagtg atggtaagcc cctaaaaaat gagggtaatc
     2221 catcacaaag ctaatggtta cgatttaatt tgacaaaacc tagaaaaaca ttataattgt
     2281 attataacaa taagtcaaga actgaaaacg gttcagttct aagatatgac aaggaggaga
     2341 ttttatgtta ggaccattta tctttattgc ttttggggtt attgtaatcc tagctattgt
     2401 tgccagtacc ctttatgttg ttcgtcaaca atctgttgcc atcgtggaac gttttggtag
     2461 ataccaaaaa acagccacga gtggtattca tatccgactt ccgtttggga ttgacaaaat
     2521 tgctgcgcgt gtgcagctgc gtcttttaca gtcagaaatc atcgttgaaa ccaaaaccaa
     2581 ggacaatgtg tttgtaactt tgaatgttgc cactcagtat cgtgttaacg aacaaaatgt
     2641 aacagatgct tactacaaat taatgaaacc tgaatctcaa atcaagtctt atattgaaga
     2701 tgccttgcgg tcatccgtgc cgaagttgac cttagacgaa ttgtttgaga aaaaagatga
     2761 aattgccttg gaagtacaac atcaagtggc agaagaaatg tcaacctatg gatatattat
     2821 tgtcaaaacc ttgattacca aggtggagcc tgatgctgaa gtcaaacagt caatgaatga
     2881 aatcaatgcg gctcaacgca aacgtgtagc tgctcaagag ttggcaaacg cagataaaat
     2941 taaaattgtg acagcagcag aagcagaggc tgaaaaagat cgattgcacg gggtagggat
     3001 tgcccaacag cgtaaggcca ttgtggatgg tttggcggag tctattcaag agcttaaaga
     3061 agcgaatatt tccttaaacg aagaacaaat catgtccatt ttgttgacca accaatacct
     3121 ggataccctc aatacctttg cggcaaaagg aaaccaaacc ctttttctac caaatacacc
     3181 aagtggggta gaggatatcc gtacacaagt tttatctgcc cttaaaacaa aataacgtgt
     3241 aaaagcttgg ctaaaaagtt gctcaatact ggatttagca gaaggctatc ataccttgtg
     3301 aagtggcaaa cgacgaaatg aatacattac ttttattgga gaaatgaaag taaaaaaagc
     3361 ctttttcatg attaacaaca tagtattgat taaatgttaa gttgtattga cttttaaatt
     3421 aaataagttt aaaatcaact taataagcaa aaaggagatt ttcaatgaaa tccaaaaaag
     3481 ttgttagtgt tatatcactt accttatccc tttttttggt gacaggttgt gctaaagttg
     3541 ataacaacaa atcagttaat cttaagcctg ctactaaaca aacctataat agctatagtg
     3601 atgaccaatt aagatcgcgt gaaaatacca tgtctgtttt atggtaccag cgagctgcgg
     3661 aaactcaagc gctttattta caggggtatc agttagcaac ggatcgctta aaagaacaac
     3721 tcaataaacc aacggataaa ccttattcaa ttgtattaga tattgacgaa acagttcttg
     3781 ataatagccc ttatcaagct aaaaatgttt tggaaggaac aggatttaca cctgaaagct
     3841 gggattattg ggtacaaaag aaagaagcaa aaccggttgc tggtgctaaa gactttttgc
     3901 aatttgcaga ccaaaatggt gttcaaattt actacatttc agacagatca actactcaag
     3961 tagatgctac aatggaaaat ctccaaaaag aaggtattcc agtacaaggt cgtgatcatc
     4021 ttctattctt agaaaaaggc gtaaaatcaa aggagagtcg tcgtcaaaag gtcaaagaaa
     4081 caactaatgt aacgatgcta tttggtgata atcttctaga ttttgctgat ttttcaaaaa
     4141 aatctcaaga agatagaaca gctttattat cagatttaca agaagagttt ggaagacgct
     4201 ttatcatttt ccctaatcct atgtatggtt catgggaagg tgccatttat aaaggtgaaa
     4261 agctggatgt gcttaagcaa ctagaggaac gccgtaaaag tttaaaaagc tttaaataaa
     4321 gtaattgaaa aagaggtgca aaagaaaatg taacctcttt ttaagtacta ttattttctg
     4381 aaaatagggt taatgactga aaaaaatgtt gattttgtga aatgattatt ttattatttt
     4441 catattttat ctttatgcta ggaattgtgg tataatggag gagtataaaa aatattaagg
     4501 agtctattca catggctaaa ttgactgtta aagacgttga tttgaaaggt aaaaaagtcc
     4561 tcgttcgtgt tgactttaat gtgcctttga aagacggcgt tatcactaac gacaaccgta
     4621 tcactgcggc tcttccaaca atcaagtata tcatcgaaca aggtggtcgt gctatcctct
     4681 tctctcacct tggacgtgtt aaagaagaag ctgacaaaga aggaaaatca cttgcaccgg
     4741 tagctgctga tttagctgct aaacttggtc aagatgttgt attcccaggt gttactcgtg
     4801 gttcaaaatt agaagaagca atcaatgctt tggaagatgg acaagttctt ttggttgaaa
     4861 acactcgttt tgaagatgtt gacggtaaga aagaatctaa gaatgacgaa gaacttggta
     4921 aatactgggc ttcacttgga gatggaatct tcgttaacga tgcatttggt acagcacacc
     4981 gtgctcacgc atcaaacgta ggtatttcag caaacgttga aaaagctgta gctggtttcc
     5041 ttcttgaaaa cgaaattgct tacatccaag aagcagttga aactccagaa cgtccattcg
     5101 tagctattct tggtggatca aaagtttctg ataagattgg tgttatcgaa aatcttcttg
     5161 aaaaagctga taaagttctt atcggtggtg gtatgactta cacattctac aaagctcaag
     5221 gtatcgaaat cggtaactca cttgtagaag aagacaaatt ggatgttgct aaagacctcc
     5281 ttgaaaaatc aaatggtaaa ttgatcttgc cagttgactc gaaagaagca aacgcatttg
     5341 ctggttacac tgaagttcgc gacactgaag gtgaagcagt ttcagaaggc ttccttggtc
     5401 ttgacatcgg tcctaaatca atcgctgagt ttgaccaagc acttactggt gctaaaacag
     5461 ttgtttggaa tggtccaatg ggtgtctttg aaaaccctga cttccaagct ggtacaatcg
     5521 gtgtcatgga cgctatcgtt aaacaaccag gcgttaaatc catcatcggt ggtggtgatt
     5581 cagcagctgc tgctatcaac cttggccgtg ctgacaaatt ctcatggatc agtactggcg
     5641 gaggcgctag catggaactt ctcgagggta aagtattacc aggtttggca gcattgactg
     5701 aaaaataata gtcattataa tatatagtaa agggcatgct gatgcatgtc tttttttagt
     5761 tgatcatgta gagttattga actttcccta acttcactta tcatttacta gtttttagat
     5821 atagtggtat ttggctgaca ctgcattttt caaaagttta tgacgatttc atatcaattg
     5881 gtttataaaa gatatcctaa tcataaataa aacgtttacc aaaagggttt tatggattga
     5941 tatgttaaaa tagatagtga agggataggt atgaaaacgt taagaaaatt attatctaat
     6001 tacaagtttg atattaaaaa gtttaaattg ggaatgagaa cattgaaaac aggcttatcg
     6061 gtctttttag ttttattggt ttttcattta tttggttgga aagggctcca aattggtgct
     6121 ctaactgctg tatttagttt aagagaagac tttgataaaa gcgtgcactt tggcttttct
     6181 agaattattg gtaatagtat aggtggccta ctctccttag tattttttgc attcaatgag
     6241 atatttcatc aagctttttg ggttacttta ttaattgttc ctatttgtac catgttatgt
     6301 atcatggtta atgttgcatg taataataaa tcaggtatta ttggggcagt ggctgctctt
     6361 ttgattatta cactgtctat tccaacagga caaacgttta tttatgtgac ttcgcgggta
     6421 tttgaaacat tttgtggcgt ctttgtagct attttagtta atactgatgt tgaacttatt
     6481 aaaaataaat ggtttaacaa aaaacacagt gatgttagat aatataacat atatacttga
     6541 caggttattt ttttcagcct ataataagga agatgaaagg agtttgtcat gaaagaaaaa
     6601 gaacttagac gctcaatggc tgtttttcct attggaacgg tgatgacatt aacagatctc
     6661 tctgcgagac aaattcgcta ttatgaagat caaggtttaa ttaagcctga acgaacgcaa
     6721 gggaatcggc gtatgttttc cttaaatgac atggaccgac ttttggaaat taaagatttt
     6781 ttgtctgagg gattaaatat tgcagctatt aaacgcgaat atgtcgagcg tcaaggtaag
     6841 ttaatgcaaa aacaaaaagc tctaacagat gcagatgtca gacggatctt acatgatgaa
     6901 atgctgacac aaagcggttt ttctactccc tcacagcata ttggtaattt tcgtatttaa
     6961 gatatgaatt gtataaaatt taaaggagac ctatttaatg gcaataacag tagctgacat
     7021 tcgtcgtgaa gtcaaagaaa aaaatgtaac gtttcttcgc ttgatgttca ctgatatcat
     7081 gggcgttatg aaaaatgtgg agattcctgc aactaaagaa cagttagaca aagtattgtc
     7141 taacaaggtt atgtttgatg gttcatctat cgaaggtttt gtacggatca atgagtcaga
     7201 tatgtacctt taccccgatt tagacacttg gattgttttt ccctggggag atgaaaatgg
     7261 agcagttgca ggtttaattt gtgatattta tacagcagaa ggaaagcctt ttgcaggaga
     7321 tcctagagga aatttaaaaa gagccctgaa acacatgaac gagatcggct acaaatcatt
     7381 taatcttgga ccagaaccag aatttttcct ttttaagatg gatgataaag gtaatccgac
     7441 acttgaagtt aacgataatg gtggttattt tgatttagcg ccaattgact tagcagacaa
     7501 cacgcgccgt gaaattgtga atattttaac gaaaatgggt tttgaagtgg aagctagtca
     7561 tcatgaagtg gctgttggtc aacatgagat tgattttaaa tatgcagatg ttttgaaagc
     7621 ttgtgataat attcaaattt ttaagctagt tgtaaaaacg attgcccgtg aacatggact
     7681 ttatgctact ttcatggcta aaccaaaatt tggaatagct ggatcaggga tgcactgtaa
     7741 catgtctttg tttgataacc aaggtaataa tgctttttat gatgaagctg ataagcgagg
     7801 gatgcagtta tcagaagatg cttattattt cttgggagga ctaatgaagc atgcttataa
     7861 ctacactgct atcactaacc ctacagtgaa ttcttataaa cgattagttc caggttatga
     7921 ggcacctgtt tatgtcgctt gggctggaag taatcgttca ccgcttatcc gtgttccagc
     7981 atcacgtggt atgggaacgc gtttggagtt acgttcggtt gatccgacag ctaatcctta
     8041 tttagccttg gctgttctct tggaagctgg attagatggt atcattaaca aaattgaagc
     8101 tccagaaccc gttgaagcta acatttatac catgacaatg gaagaacgaa atgaagcagg
     8161 cattattgat ttgccatcaa cgcttcataa tgccttaaaa gctcttcaaa aagatgatgt
     8221 ggtacaaaag gcactaggtt accatatcta cactaatttc ttagaagcaa aacgaattga
     8281 atggtcttcc tatgcaactt ttgtttctca atgggaaatt gaccattata ttcataatta
     8341 ttagacttat caggacctgc tcaagttgag tgggtctttt ggatgagcta ttgtatttga
     8401 gctagcaagc tattattagc tgatcggaca ccaattgatt ttccattata gaaaactaaa
     8461 aaccagtcca tgttctatga ggactggttt tagtttgata attatctgaa ctagaactga
     8521 tatgttaaag atatagacag atagtattaa gaaatgagtc ttatttgatt ttagtgctta
     8581 tcaggtgtta agaccatcgg aatgataatt ggttcacgct cagtcttttc atacaagaat
     8641 ggacgaagag cattgacgat agcaccgtta actgattgga tgctagcatc cttatttttt
     8701 aaagcaatac ggatagcgtt aaagagcaca cgttggcttt cgcggatcaa gtcacctgac
     8761 tcacgcatat aaatgaaacc acggctcaga atatctggtc cagctaaaat catttgtgtg
     8821 ttaaaatcaa cagttgcaac agctaagaca acaccatctt ctgacaaatc acggcggtcc
     8881 cgtaaaacgg ctgctccaat atccccgatg ccattaccat ccacgtagat gtcttgggca
     8941 ttaaagtgac cagcaatacg cgcactgtca ctggttaaag ctagaacgtc tccattttcc
     9001 ataataaaga tattttcttt tggaatacca atatccatcg ccagtcctgc atgaactttt
     9061 tgcatgcgat actcaccatg tactggcatg aaatattttg gtttaatcaa actaagcatg
     9121 agcttttgtt cttgctgtcc tccatgacca gaagtatgaa tattattgac ttttccatga
     9181 ataacatcaa caccagcttc ttggatggta ttgattaatt tattgacact tgtggtatta
     9241 ccagggattg gacttgatga gaaaatcaca gtatcaccag gctgtaaagt tacttgacga
     9301 tgagtgccat tagcgatacg agcaagtgct gccatagact caccttgact acctgtacac
     9361 ataattaaaa cttcactagc atgaagattt ttaagttcgc ttggctcaat gaaggtacct
     9421 tttggaactt taatatagcc aagttcaata ccgttaacaa ttgctttttc catagaacga
     9481 ccaaaaacgg caattttacg tcccgtttta acagcagctt ctgcagcctg ttgcaaacgg
     9541 tagatgtttg aagcgaagga agcaaaaata atacgaccat gaataccttc gataattttt
     9601 aaaatggatt ggccaacaac tttttcagag ttagtaaagg ttgggatttc agcattagtt
     9661 gagtctgata ataagcaaag gacaccttct tcaccaagcg ctgccatacg ttgtaaatct
     9721 gcaggatctc ctactggcgt gaagtcaaat ttaaaatcac cagtacagat aatcttacct
     9781 tgaggggtgt ggataacaat accgaccggt tctggaatgg aatgagtagt cttgaagaaa
     9841 gtgacgctca tgtttttaaa agttaactcg gtgttgtgat tgatttcgta aacagttgct
     9901 tcacgccaaa gaccatgttc ttccaattta ccacggataa gagcaagtgc taaaggtcct
     9961 gcgtagatag ggatattagc ctgtttgaga agaaatggaa taccaccaat gtggtcttcg
    10021 tgaccatgtg taataacaag agctttgacc cggtctaagt tatcgacaat gtaagaatag
    10081 tctggaataa cgtaatcaat tcccaaaaga tcatcttctg ggaatttgat accggcatca
    10141 acgataatga tttcgtcttg atactcaata ccataagtat tcttcccaat ctctcccaga
    10201 ccaccaatgg caaacacccc aacttcatta ggttttaaac tgatatttgt catcttagaa
    10261 ctccgttaat tcaaaaacac cggtctcttt ttcgtaatcg aggtgtttgt cagaaagtag
    10321 ctcgataaat tccacgttat aataggtgtt atcttcaacg agacgacgag ccttgatacg
    10381 gccatcgagt tcatcagtag catcaatatt aagatagagt gctttagtac tttcacgacg
    10441 tgggctttga tcctttgttt cttgatagaa aactttataa atcatttgtt tttaaattcc
    10501 tttcgttatg aatcaattat ccaaacaata ataaataggt caaaaggtag gtgttccatt
    10561 ttgacggttt tatatgttgt cattattgtg tagtagatta attcctgatt ctgtgcaatt
    10621 attacaatta taccataaac ttagctttta gtaaaaaatt taggtacatt tatggtatag
    10681 tctaaatcaa attaagagtc agcctttttg tttatgattc ttaatttgtg ttataattga
    10741 tactagaact tgcagtagaa aggcataaaa tgaagacact tgcatttgat acctcaaata
    10801 aaaccttgtc ccttgctata cttgatgatg agacacttct agcagatatg acccttaaca
    10861 ttcagaaaaa tcatagtgtt agccttatgc ctgctattga ttttttgatg acttgtactg
    10921 atcttaaacc tcaagattta gaaagaatag tggttgcaaa aggccctgga tcttacacag
    10981 gtttacgagt ggcagttgct actgcaaaaa cgttagcgta cagtttaaat attgcattgg
    11041 tcgggatttc gagtctatat gctttggctg cgtctacttg taaacagtat ccaaatactt
    11101 tggtggtgcc attgattgat gctagaaggc aaaatgcgta tgtaggttat tatcggcaag
    11161 gaaaatcagt gatgccacaa gcccatgctt cactagaagt tattatagaa caattagtag
    11221 aagaaggaca gatgattttt gttggggaga ctgctccttt tgctgagaaa attcaaaaga
    11281 aactacctca ggcgatacta cttccaaccc ttccttctgc ttacgaatgt ggtcttttgg
    11341 ggcaaagttt ggcaccagaa aatgtagacg cctttgtccc tcaatatctc aagagagtgg
    11401 aagctgaaga aaactggctc aaagataatg agataaaaga tgatagtcac tacgttaagc
    11461 gaatctaata tgaaaacagt tgaagaacaa gcaaaaaata tttatcagct tttggaaatg
    11521 gtttatggca cgtctccttg gactttagaa caagtattaa tcgatatacg gcgggatcaa
    11581 acagattatt tcctactata tgatcatgat aaattgctag gttttttagc tattcaagat
    11641 ttagctggtg aagttgaaat gactcaaatt gctatactac ctagtcatca agaacttggg
    11701 ctagcgtctc aattgatgac gcatttagat agcattgaaa gtgacatctt tttagaggtt
    11761 agagaatcta atcatagagc acagggttta tatcagaagt ttggttttaa atttattggc
    11821 aaaagaccag actactatag aaatcctata gaaacagctt tattgatgaa acgggaagga
    11881 aaacatgaca gataggtata ttttagcagt tgagagttct tgtgatgaga ccagtgtagc
    11941 gattttaaaa aacgaaagta ccctcttaag taatgtcatt gcaagtcagg ttgagagcca
    12001 caaacgcttt ggtggtgtgg tgccagaagt agctagtaga caccatgtcg aagtgattac
    12061 gacttgcttt gaggatgctt tgcaagaagc tggcatcagt gcttcagatt tatcagcagt
    12121 tgcggtaact tatggccctg gacttgttgg agccttgtta gttggattag cagctgcgaa
    12181 ggcttttgct tgggcaaatc atttaccttt gattccggtt aatcatatgg caggtcactt
    12241 aatggcagcg cgtgaacaaa agcctttagt ttatccttta atagcactgt tggtttcagg
    12301 aggacacaca gaattggttt atgtcccaga gccaggggat tatcatatta ttggcgagac
    12361 gcgtgacgac gctgttggtg aggcttatga taaagttggg cgtgtgatgg gcttaaccta
    12421 tccggcagga cgtgaaattg accagttggc tcataaaggt caagatactt atcatttccc
    12481 tcgtgctatg ataacagaag accacttaga attttccttt tcaggactca agtccgcttt
    12541 tatcaatctt catcataatg ctaagcaaaa aggcgatgaa ctgattctag aagatttgtg
    12601 tgcatctttt caagcagctg ttctagatat tttattggcc aaaacaaaaa aagctctgag
    12661 cagataccct gcaaagatgc ttgtagtggc tggtggtgta gcggcaaatc aaggattacg
    12721 agatagatta gcacaagaaa ttacacatat agaagttgtg attcctaaac taagactctg
    12781 tggagataac gcgggtatga ttgctttagc agcagctatt gagtatgaca aacagcattt
    12841 tgctaatatg agcctaaacg cgaagcctag tttagctttt gaccagtttc cagacagttt
    12901 tgtaataaac tagtcacact tgctagttag taagatttat aatggataag aatctccctt
    12961 tgaaaaaaca acccggtatc ttggcatacc gggttgtcat gatgataagt aattggcttc
    13021 tatggttact aataattcag ataattacct gcaaaataat tgtctgccta aggtgaagta
    13081 agcttaccag ctagctttag tgacgccagg aagctgtcct ttgtgagcta agtcacggaa
    13141 attgatacgg ctaacaccaa acttacgcat gtaagcatga ggacggccgt caattttgtc
    13201 gcggttcttc aagcggtttg gatttgaatc acgaggcaat ttgcgcagtg attcataatc
    13261 gcccttcgct tttaagtcac gacgaaggtc agcgtactgt tcaatcagtt ggagctgctt
    13321 ttggtattta gcaatttttg acttttttgc cattgctttc tccttcttaa tatttaataa
    13381 cctcagagtc gttatcagcg ctctgaatcc acttaactgg tatttaactg gttaagtgga
    13441 tatataatca ttatacaata aataaaagca aaagcaaata aaaaaaatta actagttatc
    13501 ttttttagcc ataatatcaa aaaactccac atctaagtgt gggagtcagg tattaataac
    13561 tgtttaaagg tgtttaatga gtgtcgcttt agcaactttg agattgaact tttaagtcaa
    13621 ataaatcagc aggaacgtag tgtttggttg agaccaagat gtctttacca ttgtagagag
    13681 tttcttggat taaaataaaa gcactatttt ctgatagagt atacttagtt gctgtgtaat
    13741 tgccagtctt ggtatagccg atttggttgc aggaatgaga agctttacca gattcataga
    13801 ttttctcttc taagaaagta agaaggggct cttcttgatt gagatttata gcatatttag
    13861 aaataacctc aatagggata aaagataaac tataagcaat agctttattt tggctatgat
    13921 accagcgatc aacaataatc acaacaggag tttcttgttt tagggatgct gtaatggcaa
    13981 cagtgggcac ttcaatccga tattccaact caacctttgt gatgtcagat gataggcttg
    14041 catatatggg gtgttgaaga tattcgaaac cttggtgata tttagtttca ggagtcttga
    14101 gtataaaatt tccctttcct ctgatatttt ttatgagatg atcctcttgg aggagggcta
    14161 gggatttacg tagtgtcatc cgactgacat tgagctgtct agcaagttca ggttctgatg
    14221 gcagttgcat gccaggacta tatgtaccat cttgaataag ttgaaaaata gtattatata
    14281 cttgcacatg tttaagtttt ttgagttttt tggttaagtc gttagtagac atatcactta
    14341 cctcattaat aatctttagt ggagtttaac ttgtctaaaa actattttag cactaacagt
    14401 attccttgat tagaattgta cccatctata gtgttagtat ctcacactca ggtacttttt
    14461 gtcaaaaaag agatgaaaaa caccttaaaa aagcgtttac aaaatagagt gtgtgtgata
    14521 ctatacaagt gtatcaaaaa ttagacaagt aaaatacagg aggtttttaa catgcaaaat
    14581 tattcaggtg aagtcggatt gcaatatcac ctacagattc gtccaggtga tgtaggtcgt
    14641 tatgttatca tgccaggtga tccaaaacgt tgtgcaaaaa ttgcagaaca ttttgataat
    14701 gcagttcttg tagcagacag ccgtgaatat gttacgtata caggtacatt aaacggtgaa
    14761 aaagttagtg ttacttcaac tggtattggt ggtccatcag cgtctattgc gatggaagaa
    14821 ttgaaacttt gtggcgctga tacctttatc cgtgttggaa catgtggtgg tattgacctt
    14881 gatgttaaag gcggagacat cgttattgca actggagcta tccgtatgga aggtaccagt
    14941 aaagaatatg ctccgattga atttcctgct gtagctgact tggaagtgac aaatgctcta
    15001 gttaatgcag ctaaaaagct tggttataca agtcacgcag gagtagttca atgtaaagat
    15061 gcattttacg ggcaacatga gccagaacgt atgccagtta gttatgaatt attaaataaa
    15121 tgggaagcat ggaaacgtct gggaacaaaa gcgtctgaaa tggaatctgc agctcttttt
    15181 gtagcagcaa gtcatctagg agttcgttgt ggatcagatt tccttgttgt tggtaatcaa
    15241 gaacgcaacg ctttgggtat ggataaccca atggctcatg atacagaagc agctattcaa
    15301 gttgctgttg aagcactccg cacacttatt gaaaatgata aatcacaata agcgaggttt
    15361 tggaagtcat catgcaattt atttatagta ttattggtat tttattggta ttaggaattg
    15421 tgtatgcaat ttctttcaat cgtaagagtg tttctctaag tttaattgga aaagctctta
    15481 tcgttcaatt cattattgcg ctaatcttag tacgtatccc actaggccaa caaattgtta
    15541 gtgttgtttc aactggagtt actagcgtaa tcaactgtgg tcaagctggt ttaaattttg
    15601 tgtttgggtc attagcagat agtggcgcaa aaactggttt tattttcgct attcaaacgc
    15661 ttggtaatat tgttttctta tctgccctag ttagtctact ttattatgta ggaatccttg
    15721 gatttgtagt aaaatggata ggtaagggcg ttggtaaaat tatgaaatcc tcagaggttg
    15781 agagttttgt tgctgtagct aatatgtttc ttggtcaaac agacagtcca atcttggtta
    15841 gcaaatacct aggtcgtatg actgatagtg agataatggt tgtgttggta tcaggtatgg
    15901 gaagtatgtc agtttctatt cttggtggct atattgcatt aggcattcca atggaatatc
    15961 tcttgattgc ttcaacaatg gttcctattg gcagtattct cattgctaaa atcttattgc
    16021 ctcaaacaga acctgttcaa aaaattgatg acattaagat ggataataaa ggtaataacg
    16081 ccaatgtgat tgatgcaatc gctgagggtg caagcacagg tgcacaaatg gctttctcaa
    16141 ttggtgctag tttgattgcc tttgttggtt tagtttcttt gattaatatg atgttaagtg
    16201 gattgggaat ccgcttagaa caaatctttt catatgtttt tgctccattt ggttttctta
    16261 tgggatttga ccacaaaaac attcttctag aaggaaacct tcttggaagt aagttgattt
    16321 taaatgagtt tgtttcgttc caacaattgg gtcacctaat caaatcttta gattatcgta
    16381 cagcattggt agcaactatt tcactctgtg gttttgctaa tttatcaagt ttaggtattt
    16441 gtgtttcagg tattgctgtt ctttgcccgg agaaacgtag caccctagct cgacttgttt
    16501 tccgtgcaat gattggtggt attgctgtaa gtatgcttag cgcctttatc gtcggtattg
    16561 taactctatt ctaaacgttg acaaaagaaa gaaggatagt aacgtggaag taaaagatat
    16621 tttaaaaacg gtagaccata ctttgctagc aacaacagca acgtggccag aaatccaaac
    16681 aattttagat gatgccatgg cttatgaaac agcttcagca tgtattccag cttcttacgt
    16741 caaaaaagca gcagaatacg tttcaggtaa attagctatt tgtactgtta ttgggttccc
    16801 aaatggctat agtacaactg cggcgaaggt ttttgaatgt caagatgcta ttcaaaatgg
    16861 tgctgatgaa attgacatgg tcattaattt gacagacgtt aaaaatgggg attttgatac
    16921 tgttgaagaa gaaattcgtc aaatcaaagc taaatgtcaa gaccatatct taaaagttat
    16981 cgttgagaca tgtcaattaa ctaaagaaga acttatcgaa ctttgtggag ttgtcacacg
    17041 ttcaggtgca gactttatta aaacctctac tggtttttcg acagcaggtg ctacatttga
    17101 agatgttgaa gtgatggcaa aatatgtcgg cgaaggtgtt aaaattaagg cagcaggtgg
    17161 aatctcatca ttggaagatg ctaaaacatt tattgcttta ggagcttcac gcttgggtac
    17221 tagccgtatc atcaagattg ttaagaacga agctacaaaa accgatagct attaacttaa
    17281 tcataccgat ttagcttgag actaaaacaa aaaactcata taataggagt taaaaagcct
    17341 actatcttag tgataatagg cttttttagc tatgaagact tccctgcgat ccatcctgaa
    17401 caaagagctg aggtaatatt aaaccctccg gtatgagcat taatatctaa gacctctcca
    17461 gcgaaataca ggccaggaac tttcttgctt tccaaggttt tagggttgat ttcttttaaa
    17521 tctacgcctc ctttggtcac aaatgattta gccaaagaca tttttcctgt aatgggtatc
    17581 tggaggtgtt tgagtttatc aagcaattcc ttttcttgtt ttggagagag ttgtttgacc
    17641 ttttcggggt aatcttctga taaaaaatca gctactcgtt caggcagtag gcctttaaga
    17701 gcatttttga tgtttttatc acgttgatca ctcagataag cggttaagtc atctgttgat
    17761 aagtgtggca aaaagtccag ctctgcaatt tctccacctt tcacaaaaga ggacagacga
    17821 agagctgcag gtcctgaaag gccaaagtgg gtgaagagta agtcgtgtgt gatgacgtgt
    17881 ttgtcataac taagagtaac gtcgtctagg gaaatacctt gcaagacttt gtgaggaaaa
    17941 tcagttaaca gagggctctc agcagcttct aaatcagtca cggtcagttt aaaatgccgt
    18001 gcaatatcat gtccaaaacc tgttgaaccc gttgaagggt aggacttgcc tcctgtggtc
    18061 acaatcaatt tttggcaggt gaaagtctgg tctgcagatt tgaggtagaa gaggtcatct
    18121 tgttttttaa cggaaaccac ttcagtgctg gttagcacct gacccccaag agccttgatt
    18181 tttttctcca gagcatcaat gattgtcctt gatttatctg ttgtggggaa catccgtcca
    18241 tggtcttctt ctttgagttt gacgccgttt tcctcgaaaa aggcaatgat gtcatggttg
    18301 tcaaactggg aaaaaacact atataaaaag cggccatttc caggaatgcc agccataagg
    18361 acatcaaggt taccactatt agtgacatta cagcgtccac caccggttcc cgctaatttt
    18421 ttgcctaagc ggcgattttt ttcaattaag agagttttat agccatagta gctgctggag
    18481 atggcggcca tcatgccagc agggccacct ccaataataa tcgtatcgta ttgagtcata
    18541 gccttatttt agcataaaag cgggggattt aatagtcagg taaatgaact aacatggcat
    18601 accgtttttt acgcatttaa acaatgcttt caagtaataa ttgatgcctt tggcacgact
    18661 gaaatgaggt caaattgaca gaatactcct attatggtac tatttttaat aagaatattc
    18721 agtgatagtg ataaagagga aaatatggga attgaaaaga cagttagtga actagctgat
    18781 attttgggag tgagtcgtca ggccgtcaat aatcgtgtta agtctttacc cgaagaagat
    18841 cttgacaaaa atgaaaaagg cgttactgtg gttaagcgca gtggtcttgt taagctggaa
    18901 gagatctata aaaaaacaat ttttgacgat gagcctatca gtgaagaaac gaaacaacgt
    18961 gagcttttag agattctcgt ggatgagaag aacactgaaa tcacgcgcct ttatgagcaa
    19021 ctcaaagcca aagatgctca acttgcctca aaagatgagc aaatgcgtgt taaagacgtg
    19081 cagatcgctg aaaaagataa acaattggat cagcagcaac aattaactgc taaagccatg
    19141 gctgataaag aaaccctaaa attagaattg gaagaagcta aagcagaagc caaccaagct
    19201 cgcttacaag tcgaagaagt tcaggctgaa gttggcccta aaaaaggctt tttcacccgc
    19261 ttgtttgcga aataatcaca aaaaatgacg aaggccctat cagggtcttt tttggaggaa
    19321 aacatgaaac acttagatac ttatattgcc tttgatttag aatttaacac tgtcaatgac
    19381 gttagtcata tcattcaagt atcagcagtc aaatatgacc accataaaga agttgacagc
    19441 tttgacacct atgtttacac agatgttccc ttacagagct ttatcaatgg cctaacagga
    19501 atcacatctg acaagattgc tgcggaacca aaagtagagg aggttatggc tgcgtttaaa
    19561 aattttgtgg gagagttacc actgattggt tataatgctc aaaaatctga cctgcctatt
    19621 ctagctgaaa atggcttgga tttaagggac caataccaga ttgacctctt tgatgaagct
    19681 tacgatagac gtagcgcaga cttaaatggg attgctaacc ttcgtctgca aacagtggca
    19741 acctttttag gcattaaggg tcgaggtcat aatagcttag aagatgccag aatgacagca
    19801 gtaatctata agtctttttt agaaacagat acaaacaaag catatctcag ccagcaagaa
    19861 gaggtaacca cagataaccc tttcgcagct ttgggagact ttttcgacta aaggctctgc
    19921 tatttttgaa aaataaaaaa attcctaggg ttagcccttg cttataacgc taggtcatgt
    19981 aagatagggg catatcaaga gggagtcggg tgcacacgag ttgcttttga tcactaaatg
    20041 atagaaagaa ggtgtggaaa tgtctcaatt ctattcgact ggagaattgg ccaacttagc
    20101 tggcgtttct attcgaactg tccaatacta tgaccaacgt gggattttga ttccgacagc
    20161 attgacagca ggtggacggc gactctatac ggattctgat ttagaacaat tacgcatgat
    20221 ttgctttctg cgcgatttgg gattttccat tgagcagatt cgcaaagtct tagcagagga
    20281 aaatgcagcc caggtgctgg aattgcttct agttgatcat attgcgactg ccaaggaaga
    20341 cttggctgca aaagagcaac aagttgacat agctgtcaag atacttgaca gacttcgaaa
    20401 acaagacccc cagtcactag attttctgat ggacatttcg cttagcatga aaaatcaaaa
    20461 agcttggaaa aaattacaat ggaagatggg tatcagtata tttttagcca ttgtttcata
    20521 tgtaggactt attttactat ctagttatct gcaaaaattt tggttagttt atgttgctat
    20581 gggactattt ctaccaggtt tcagttggtt ggtaatttat tttaggaagc aatttattta
    20641 tctctgtccc cattgtcatc agaaatttga acctagcttt aatgaatttg cccttgcagg
    20701 ccatacacca cgaaccagaa agttaacctg ctcccattgc catcaaaaat cgtattgctt
    20761 ggaactggca aaagaggaca ctgtctcaag aagataagca ctaactcata aaaaaactag
    20821 cctgagatcc ttctcaagct agtttttgtt tttttagaat ggtaatttac cagcaaaagc
    20881 gcctaatgtt ttcttagttg tttcatcaat ttgcgtaagg gcatcattaa tggcttgagt
    20941 ggtcatgtct tgaagcgttt ctacatcttc aggatctaca acagcttctt tgaagtcaat
    21001 cccaaccaat tttttatcac ccgtgaaagt ggcagtgaca aggtcttgag ctgattttcc
    21061 agtaaattgc atagcagcca aatccgcctg cttttgctcc atttgtttct gaagcttttg
    21121 tgcttgcttc atcatgtttt gcatgttcat cataagacta tactctcctt aatttaatac
    21181 taactattat aacacataaa aagaggcaat aaaaacaatg tcagttagta gactagcctg
    21241 tatcagtaag aaagtcgcta tggataagat ggattagcag caagggtctg gctaatgaca
    21301 gaataagtct agaaatgaac tatttttgtc atagactagt ccgacttagt ggttatctaa
    21361 ttcacgatct tctttagctt gttgttctaa aagaatttgt gcgaagtcaa cccacttagt
    21421 gtagttattt tcggataatt gttctgaaat catcgctaac tctcgttgtg gcaactgata
    21481 agagactccc ttattttcta aggttaaatc ttcttccatt agggcggtta aactaacatc
    21541 aaagatagca gcaatatcac ttaatagacc agactttgga gtatattttc ctttttccca
    21601 ctcactgatt gaagaagagc tttttcgtcc taggagatta gcaagttcta attgttccat
    21661 gttctttttt aatctaaggt actttaaatt acttgcaaaa aaagttttct tggcgg
//
LOCUS       DAXWIQ010000029        21714 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000029 DAXWIQ010000000
VERSION     DAXWIQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21714
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.029"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(178..414)
                     /locus_tag="ACGEUI_001147"
     CDS             complement(178..414)
                     /locus_tag="ACGEUI_001147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8165987.1"
                     /translation="MVERSYNNVGNFLKEYFGGFFEIINFDDLRLTMVDMVSLSSEYD
                     VIVTDMILEQTMDSEILFFNQMAPSVVANRLTDM"
     gene            complement(634..1101)
                     /gene="perR"
                     /locus_tag="ACGEUI_001148"
     CDS             complement(634..1101)
                     /gene="perR"
                     /locus_tag="ACGEUI_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HFF8165988.1"
                     /translation="MDIHSHQQALDAYENVLEHLREKHIRITETRKAIISYMIQSTEH
                     PSADKIYRDLQPNFPNMSLATVYNNLKVLVDEGFVSELKISNDLTTYYDFMGHQHVNV
                     VCEICGKIADFMDVDVMDIAKEAHEQTGYKVTRIPVIAYGICPDCQAKDQSDF"
     gene            complement(1153..1608)
                     /locus_tag="ACGEUI_001149"
     CDS             complement(1153..1608)
                     /locus_tag="ACGEUI_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFF8165989.1"
                     /translation="MIYSFQNPSEDILKAYLESAKTIAVVGLSDRKDTAAYGVAKFMQ
                     AMDYRIIPVNPKLAGQLILGEKVYASIKAIPFEVDIVDVFRRSEFLPEVARDFLAGQA
                     KVFWAQLGLENQEVQTILRSAGKEAIVMNRCLKIDYLQLVAKPECITNL"
     gene            complement(1795..4437)
                     /gene="polA"
                     /locus_tag="ACGEUI_001150"
     CDS             complement(1795..4437)
                     /gene="polA"
                     /locus_tag="ACGEUI_001150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFF8165990.1"
                     /translation="MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGF
                     HLMLDHMMKRVRPTHVLVAFDAGKTTFRTEMYADYKAGRAKTPDEFREQFSYIREMLT
                     ALGIAYYELEHYEADDIIGTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKK
                     GVAEFEEFTPAYLMEKMGLTPNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGS
                     LEGIYEHIDGFKTSKMKENLINDRDQAFLSKTLATINTASPITIGLDDIVYNGPDVAS
                     LSQFYDEMDFVQLKKGLASQMPQEPVAVISYQEVTNVSADLFSAEDIFYFETLRDNYH
                     REAIIGFAWGHGEQIYASTDLSLLATDSFKQVFQKPIATYDFKRSKVLLSHLGIELVA
                     PSYDARLANYLLSTVEDNELSTIARIFTDISLEEDDTVYGKGAKRAVPDKDVLLEHLA
                     RKVKVLLDSKSQMLDKLTAHEQLDLYQNIELPLANVLAKMEIEGIKVNRATLQDMAEQ
                     NKVIIEALTQEIYDMAGQEFNINSPKQLGSILFEKMQLPLEMTKKTKTGYSTAVDVLE
                     RLAPIAPIVAKILDYRQITKLQSTYVIGLQDYILADGKIHTRYVQDLTQTGRLSSVDP
                     NLQNIPIRLEQGRLIRKAFTPSHEDAVLLSSDYSQIELRVLAHISGDEHLIAAFNEGA
                     DIHTSTAMRVFGIDKAADVTANDRRNAKAVNFGIVYGISDFGLSNNLGITRKQSKSYI
                     DTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDINSRNFNVRSFAERTAINS
                     PIQGSAADILKIAMINLDKALQAGGFRAKMLLQVHDEIVLEVPNDELTAIKKLVKDTM
                     EAAVDLAVPLCVDESTGQSWYEAK"
     gene            complement(4568..6295)
                     /locus_tag="ACGEUI_001151"
     CDS             complement(4568..6295)
                     /locus_tag="ACGEUI_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFF8165991.1"
                     /translation="METILQTKLPVAQLVEQLTEWLTKTFSGLFDIMQVVGSFLMDWM
                     TKTLLFIHPLLFIVLVTAGMFFLAKKKWPLPTFTLLGLLFIYNQGLWKQLMNTFTLVL
                     VASLISVLIGIPLGIWMAKNATVRQIVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLAIRDIPTELIEASDAFGSTGKQKLFKVELPLAKNTIMAGVN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGSGFVSGLALVILAIVLDRMTQLF
                     NSKPQEKAKAGKTNKWIGLAALAVFLIAALGRGIMTMASGMADKGETVNIAYVQWDSE
                     VASTHVIAEVLKNEGYHVTLTPLDNAVMWQTVANGNADFSTSAWLPVTHGQQYQKYKS
                     KLDDLGPNLKGTKLGLAVPKYMTDVNSIEDLSKQADQKITGIEPGAGIMAAAKKTLKE
                     YHNLSSWELVAASTGAMTTSLDQAIKKKDPIVVTAWSPHWMFAKYDLKYLKDPKETFG
                     STENINTIARKGLKKDLPNVYKIIDKFHWTQKDMEAVMLDINKGMSPEAAAKKWVEAN
                     KSKVSSWTK"
     gene            complement(6311..7507)
                     /locus_tag="ACGEUI_001152"
     CDS             complement(6311..7507)
                     /locus_tag="ACGEUI_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8165992.1"
                     /translation="METILEVKHLSKIFGKKQKAALEMVKTGKNKSEIFKKTGATVGV
                     YDASFEVKKGEIFVIMGLSGSGKSTLVRMLNRLIEPSAGSILLEGKDISTMSADQLRE
                     VRRHDINMVFQSFALFPHKTILENTEFGLELRGVPKEERQRLAEKALDNSGLLDFKDQ
                     YPNQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDSMKQTI
                     IFISHDLNEALRIGDRIALMKDGQIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTTVELDGPQVALNRMHNEEVSMLMATNRRRQLVGSLTADAAIEARKKGL
                     PLSEVIDRDVRTVSKDTIITDILPLIYDSSAPIAVTDDNNRLLGVIIRGRVIEALANI
                     SDEDLN"
     gene            complement(7995..9086)
                     /gene="ulaG"
                     /locus_tag="ACGEUI_001153"
     CDS             complement(7995..9086)
                     /gene="ulaG"
                     /locus_tag="ACGEUI_001153"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFF8165993.1"
                     /translation="MTKVQDITRESWILNTFPEWGTWLNEEIEQEVVPADNFAMWWLG
                     NCGIWIKTPGGANVVMDLWSNRGKATKQVKDMVRGHQMANMAGARKLQPNLRAQPMVI
                     DPFMINELDYYLVSHYHSDHIDINTAAAIINNPKLNHVKFVGPYECGEVWKNWGVPKD
                     RIMILKPGDSFEFKDIKITAVESFDRTCLVTLPIQGADAQDGDLAGLAITDDDMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGRDYDIDVVLNNYGENPIGIQDKMTSVDLLR
                     MAENLRAKVVIPVHYDIWSNFMASTDEILELWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DQNRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            complement(9339..10985)
                     /locus_tag="ACGEUI_001154"
     CDS             complement(9339..10985)
                     /locus_tag="ACGEUI_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8165994.1"
                     /translation="MMILDKKSYDLLSYLLKLETPETVMAISHALNQSRRKVYYQLDK
                     INQALPKGVDQIISYPRLGILLTADQKAACRLLLEEVTDYNYVMKSDERRRLSSIYIA
                     VSTERVTIDKLMQINDVSRNTILNDLTELREELEDKQYKIQLHATKARGYYFGCHPMA
                     LIQYLYKLLVDVYQGGNTSFIDIFNRKLSEIQGLSVYFSKDILTYFHEYLFLSQASLG
                     KTINTQDSQFMLQILPFMLLSYRNMRLDSETKSALKQEFHLIWKRKEYHIAQDLAREL
                     YHNFKLHLDDIEVSMVAMLMLSFRKDQDHHVESQDYDDMRATISHFIDQLESRYQLHF
                     THKQDLLKQLTTHCKALVYRKAYGIFLVNPLTDHIKEKYEELFAMTQSCATILEQDWT
                     ISLTDDDIAYLTIHLGGELRHNNTEQEKTKLVIVSDDGIGIQKLLFKQCQRYLANGQI
                     EAVFTTEQYQSVYDLLAVDMIVATTDTLKTKIPMLIVNPILSDDDIIKLIRFSKQGRL
                     SEHSRFSTELTKAIEAVVKDESDRYALVSKIEKLIHRELL"
     gene            complement(11310..12014)
                     /locus_tag="ACGEUI_001155"
     CDS             complement(11310..12014)
                     /locus_tag="ACGEUI_001155"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFF8165995.1"
                     /translation="MAKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYDLLTPENMVVTDLDGNVVEGDLNPSSDLPTHVELYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTKAEVDGAYEQETGNVILEEFSKRGL
                     DPMAVPGIVVRNHGPFTWGKTPEQAVYHSVVLEEVARMNRLTEQINPRVEPAPRYIMD
                     KHYLRKHGPNAYYGQK"
     gene            complement(12016..12879)
                     /locus_tag="ACGEUI_001156"
     CDS             complement(12016..12879)
                     /locus_tag="ACGEUI_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFF8165996.1"
                     /translation="MARPIGIYEKATPKQFTWRERLQFAKDLGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGIRIPTICFSGHRRYPLGSNDPAIEAKSLKLMKQCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPETRARFIKNLRQSCDWAEEAQVMLSIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDAGNVSAWHNDLWSEFYNGHKSIAALHLKDTYAVTET
                     SKGQFRDVPFGQGCVDWQELFAVLKKTNYNGPFLIEMWSENCDTVEETKAAIKEAQDF
                     LYPLIEKAGLA"
     gene            complement(12884..13546)
                     /locus_tag="ACGEUI_001157"
     CDS             complement(12884..13546)
                     /locus_tag="ACGEUI_001157"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFF8165997.1"
                     /translation="MTHIPNLQVALDHSDLQGAVKAAVAVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFPEKIIVADTKCADAGGTVAKNNAKRGADWMTCICCATIPTMEAALKA
                     MKEERGDRGEIQIELYGDWTYEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KTLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAEDPAAAARAFKDEIKRI
                     WG"
     gene            complement(13637..14122)
                     /locus_tag="ACGEUI_001158"
     CDS             complement(13637..14122)
                     /locus_tag="ACGEUI_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8165998.1"
                     /translation="MNLKQAFIDNNSIRLGLSADTWQEAVQLAVQPLIDSKAVTSAYY
                     DAIIASTEKYGPYYVLMPGMAMPHAEAGLGVNRNAFALVTLTKPVTFSDGKEVSVLLT
                     LAATDPSIHTTVAIPQIVALFELDNAIERLVACQSPKEVLEMVEESKDSPYLEGMDLN
                     A"
     gene            complement(14245..14523)
                     /locus_tag="ACGEUI_001159"
     CDS             complement(14245..14523)
                     /locus_tag="ACGEUI_001159"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8165999.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEA
                     KGLASGYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEVL"
     gene            complement(14594..16027)
                     /locus_tag="ACGEUI_001160"
     CDS             complement(14594..16027)
                     /locus_tag="ACGEUI_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8166000.1"
                     /translation="MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAG
                     FVKATVGYLILNVGAGGLVTTFRPILVALAKKFELKAAVIDPYFGLAAANTKLEEMGF
                     ISVATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNA
                     FGAWAVGIICGLYWAISSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGAILAVLGPDIMSDVDLIGPGAFNPAKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVSELTNAFQGISSKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVIFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGILQVALGAVAVGLLGLTGGYHGNIDLVLPWLPFGYLFKFLGIAGYVLV
                     CIFLLAIPQLQFAKAKDKEAYYRGEAQ"
     gene            complement(16334..18835)
                     /gene="leuS"
                     /locus_tag="ACGEUI_001161"
     CDS             complement(16334..18835)
                     /gene="leuS"
                     /locus_tag="ACGEUI_001161"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFF8166001.1"
                     /translation="MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTSDQ
                     AEAVADYKRQASLKSDLARTDLAKEKTGVWTGSYAINPVNGKEMPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFNLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASA
                     IAKMVEWLEAEGVGNENVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANVTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK"
     gene            complement(19046..20185)
                     /locus_tag="ACGEUI_001162"
     CDS             complement(19046..20185)
                     /locus_tag="ACGEUI_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFF8166002.1"
                     /translation="MRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHL
                     YTRFSNPTTEALEDGLACLEKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGT
                     CQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELAQL
                     AKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYN
                     QLKLFQKNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYP
                     GLDSHPNHDTHLQQAKNGGAVLSFELASKEELMSFTHRIQLPILAVSLGGVESILSHP
                     ATMSHACLSSQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF"
     gene            20679..20966
                     /locus_tag="ACGEUI_001163"
     CDS             20679..20966
                     /locus_tag="ACGEUI_001163"
                     /inference="COORDINATES: protein motif:HMM:NF043072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoV family signaling peptide"
                     /protein_id="HFF8166003.1"
                     /translation="MKNKLFLVALATVTVLGPSLATPHHQTVHAAEQAKEPQTSSMTC
                     TNDGSVCAGWHSLSEGDSITSNSSSNTKTSLLNWLWSLITNLWNSAKNLFI"
     gene            21141..21308
                     /locus_tag="ACGEUI_001164"
     CDS             21141..21308
                     /locus_tag="ACGEUI_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoV family signaling peptide"
                     /protein_id="HFF8166004.1"
                     /translation="MKKKLSLFMIATAAVVSLSLATLGSHQTVSANDASYSNGFYGHT
                     GPDSWLLYTVW"
BASE COUNT         6451 a         4756 c         4133 g         6374 t
ORIGIN      
        1 actaagaatc tctttttcga gctttttgtt ttttaatttt tcaagttgaa taattaaacc
       61 ttgcttttct ttttcaaggt aatagactct actccttgta agtctaactt attcttaatc
      121 ttatttatcg ctcatttttt aaattccgat gctctcacag atattatcat tgtttagtta
      181 catgtctgtc aatctattcg ccaccacact aggggccatc tgattgaaaa ataaaatttc
      241 ggagtccatt gtttgctcaa gaatcatatc tgtcacaatt acgtcatact ctgaagacag
      301 tgacaccata tcaaccatgg tcagcctcaa gtcatcaaag ttgataatct caaaaaatcc
      361 tccaaaatac tcttttaaga agttgccaac attgttataa cttctctcaa ccactaatag
      421 tttcaagcga cgacgcgatt gatttgtaag aaagagattt tcccaggtga ggaacaaaca
      481 aatagaggag atgattgatg gttctttcct ttaattcctt atgatcccca gcaaataact
      541 ctttaacata agcaacaaag gtctggtatt gaactggggg tttttgattt gtaaaggcac
      601 tatatttata atgcctttat ttaagggtta actctaaaaa tcagattggt cttttgcttg
      661 gcaatccgga caaatcccgt aagcaataac tgggatacgc gtgaccttat aacctgtttg
      721 ctcatgggct tctttggcaa tatccataac atcaacatcc ataaagtcag caatcttccc
      781 acatatttca caaactacat tgacgtgttg atggcccata aagtcatagt aggtggttaa
      841 atcattgctg attttcaact ctgagacaaa accttcatca actaaaactt tcaaattatt
      901 atagactgtg gcaaggctca tgttaggaaa gttaggctgt aagtcacggt aaatcttatc
      961 ggcactaggg tgttctgtgg actgaatcat gtaagaaata atggctttac gtgtctctgt
     1021 aattcgaata tgtttttccc gtagatgctc taggacattt tcataggcat ctagggcttg
     1081 ctgatgtgaa tgaatgtcca taagctgcta ctcctatcct tttttccttt attatcacgc
     1141 acgtctctag tttcaaagat ttgtgatgca ttctggttta gcgactaatt gcaaatagtc
     1201 tatttttagg cagcgattca tcacaatagc ctctttgcct gccgaccgta aaatggtttg
     1261 tacttcttgg ttttcaaggc ctaattgcgc ccaaaagact ttggcctgac ctgctaaaaa
     1321 gtctctggca acttctggta aaaactcact tctgcgaaag acatctacaa tgtcaacctc
     1381 aaagggaatt gccttgatac tggcatagac cttctctccc aaaataagct gacctgctaa
     1441 tttgggattg actggaatga tccgataatc catagcctgc ataaatttgg cgacaccata
     1501 agctgctgtg tctttgcggt cagaaagacc aacaaccgca atggttttgg ctgactccaa
     1561 gtaagctttt aggatatcct ctgagggatt ttgaaacgaa taaatcactt gttcccctat
     1621 ctatttaata acctctacag ctgttcaata tagcaggata actcctaaag catggcttca
     1681 atcactttta accttatccg tcaaaaagct caaagcagct tcatgacttc tcctacaatt
     1741 ttttagttta ttataacata agttgtcaaa atgagaaata acaagtaaga aaacttattt
     1801 ggcttcgtac cagctctgcc cagtactttc atcgacacat aaaggaacag ctaaatcaac
     1861 agcagcttcc atggtgtctt tgacaagttt ttttatagca gtcaactcat catttggcac
     1921 ttccaaaacg atttcatcat gcacttgaag cagcatttta gctctaaaac cacctgcttg
     1981 caaggcctta tccagattaa tcatagcgat tttgagaata tcagctgcgc ttccttgaat
     2041 gggcgagttg atggctgtac gctccgcaaa agagcgaacg ttgaaattac gagagttgat
     2101 atccggtaat tcacggcgac gcttaaagag ggtctctaca tagcccttgt ctttagcctc
     2161 acgcaccaca ttttccatat aggctttaat cccaggatag cgttcaaaat aggtatcaat
     2221 gtaagacttg gactgtttac gggtgatacc aaggttattt gacaaaccaa aatcagaaat
     2281 cccatacaca attccaaagt taacagcctt ggcattacgg cggtcattgg cagtaacatc
     2341 tgctgccttg tcaatcccaa acacacgcat agccgttgag gtatgaatgt ctgccccttc
     2401 attaaaggca gcaatcaaat gctcatcccc tgaaatatgt gctagcacgc gcaattcaat
     2461 ctgtgagtaa tctgaactca acaagacggc atcttcatga gatggggtaa aggccttacg
     2521 aatcaaacgc ccctgttcca aacgaatggg aatgttttga agattagggt ccacactaga
     2581 caaacgccct gtctgggtga gatcctgcac atagcgggta tgaatcttgc catcagccag
     2641 aatataatcc tgtaaaccaa tcacataggt ggattgcaat ttagtaatct ggcggtaatc
     2701 caaaatttta gccacaattg gagcaatcgg tgccaaacgt tccaaaacat caacagctgt
     2761 agaataacct gttttggtct tcttagtcat ctcaagaggt agttgcatct tctcaaacaa
     2821 gatactgccc agctgtttag gcgaattaat gttaaattct tggccagcca tatcataaat
     2881 ctcttgggtg agcgcttcaa taatgacctt gttttgctct gccatatctt gcaaagtcgc
     2941 cctattaacc ttgatccctt caatttccat tttagcaagg acattagcca aaggcaattc
     3001 aatattctga tagaggtcta gttgctcatg ggcagtgagc ttgtctagca tttgagactt
     3061 gctatctaat aatactttga ccttacgagc taagtgttct agtaagacgt ctttatcagg
     3121 aacagcccgt ttagcacctt taccatagac cgtatcgtct tcttccaaag agatgtcagt
     3181 aaaaatgcgg gcaatggtgc taagctcatt gtcttctaca gttgacagaa ggtaattagc
     3241 tagtctagca tcatagctag gtgctaccaa ctcaattcct aagtggctca acaagacctt
     3301 gctacgttta aaatcataag tggcaatggg tttttggaag acctgcttaa aactatccgt
     3361 cgctaatagg ctaaggtctg tggatgcgta aatctgctca ccatgtcccc aagcaaaacc
     3421 aatgatggcc tcacgatggt aattatcccg aagcgtttca aagtagaaga tgtcttctgc
     3481 gctaaacaaa tcagcagaca cattggtcac ttcttgatag gaaatgacag ctactggctc
     3541 ttgaggcatc tggctagcaa gacctttctt gagttgcaca aaatccatct catcgtaaaa
     3601 ttgagagagg ctagcaacat ctggaccatt gtagacaata tcatctaagc caattgtgat
     3661 aggagatgct gtattgatag tggcgagcgt tttagatagg aaagcttggt cacggtcatt
     3721 gatcaagttt tctttcattt ttgaggtctt gaatccatca atatgctcgt aaatgccttc
     3781 caaactacca aattcatgaa ggagcttaag gcctgttttt tctccaattt ttgtcactcc
     3841 aggaatgtta tccgatttat ctcccatcaa ggctttgagg tcaataaact ggtttggcgt
     3901 caagcccatt ttttccatga ggtaagccgg tgtgaattct tcaaattcgg caaccccttt
     3961 tttagaaatt tcaaccacgg tattttcgtc agttaactgg atcaaatctt tatcaccact
     4021 gacaatggtc acatcaaaag gcacttctgt gcgctccgcc attttatcaa gcgtcccgat
     4081 aatatcgtct gcttcgtagt gttccaactc ataataagca atcccaaggg ctgtcaacat
     4141 ctcacgaata taagaaaact gctcacgaaa ttcatctggg gttttagcgc gacccgcctt
     4201 ataatcggca tacatttctg ttcggaaggt ggttttgccc gcatcaaaag ccaccagcac
     4261 gtgagtaggc cggactcttt tcatcatgtg gtctagcatc aaatgaaagc cataaatggc
     4321 attggtatgg agaccgctat gatttttaaa gcggtcaatt tgattgtaaa gggcaaaaaa
     4381 ggcacgaaag gcaacagagg agccatcaat taatagtaat ttattcttgt tttccatagc
     4441 cttattataa cacagaggga cgggcttttc tcctttaagc cattggtttt gctgataagt
     4501 aaatggttaa cctaaaaagc ctaggaaaat tcctagactt ctgtatttgc tatttagaca
     4561 aaagcactta ttttgtccaa cttgatactt tagatttgtt tgcctctacc cattttttgg
     4621 cggcagcttc tggactcatg cctttattga tgtcaagcat gactgcttcc atatcttttt
     4681 gggtccagtg gaacttatca atgattttgt agacatttgg aagatctttt ttcaagcctt
     4741 tacgagcaat ggtattgatg ttctcagtcg agccaaaggt ttctttagga tctttaaggt
     4801 acttcaagtc atacttggca aacatccagt gtggtgacca agctgtcacg acaataggat
     4861 cttttttctt gatcgcttga tccaaggaag tcgtcatggc tcctgttgat gccgccacta
     4921 actcccaact agatagatta tgatactctt ttagagtctt tttggctgct gccatgatac
     4981 ctgcacctgg ttcgatccct gtgatttttt gatcggcttg tttgcttaaa tcctcaatac
     5041 tattaacatc tgtcatgtat ttaggaacag ctaaaccaag tttggtccct tttaggttag
     5101 gtcctaaatc atctaatttt gacttgtatt tctgatattg ttggccatgg gtgactggca
     5161 accaagcact agttgaaaaa tcagcgttcc catttgcaac ggtttgccac atgacagcat
     5221 tgtcaagcgg tgtcaaggtt acatggtaac cttcgttttt caaaacttct gcaatcacat
     5281 gagttgaggc aacctcagaa tcccactgca cgtaagcgat gttaacagtt tcacccttat
     5341 ctgccatacc agaagccata gtcataatac cgcgtcctaa agcagctatc aaaaagacag
     5401 caagggctgc tagaccaatc catttattgg ttttgccagc tttggctttt tcttgaggtt
     5461 tgctattgaa caactgtgtc atacggtcaa gaacaatcgc caagataacc aaggcaagac
     5521 cactgacaaa tccactacca atatcagcgt gttgaagggc tgacaagact tcacgtccta
     5581 aaccaggagc accaatcatg gagcctgtta ctaccatgga aagggctaac atcatggtct
     5641 gattaacacc tgccataata gtatttttag ctaaaggcaa ttcaacttta aagagctttt
     5701 gtttcccagt tgacccaaag gcatctgagg cctcaatcaa ttctgttgga atgtcacgaa
     5761 tggccaaatt tgtaaaacga actgttgggg gcaaggcaaa gatgactgaa gcaaatactc
     5821 caggcaccat accaatacca aaaaaggcaa cggcaggaat caagtaaaca aaagccggca
     5881 tggtttgcat aaagtccaaa ataggattca caatctgacg gacagtggca tttttagcca
     5941 tccaaatccc cagcggaata ccaataagta ccgaaatcaa actagctact aacaccagag
     6001 taaaggtatt cattagctgc ttccataacc cttgattgta aataaagagc aatcctaaca
     6061 aggtaaaggt tggcaaaggc cattttttct tagcaaggaa aaacatccct gctgttacta
     6121 gaacaataaa aagtaacggg tggataaata aaagtgtttt ggtcatccaa tccatcaaaa
     6181 agctaccaac tacttgcata atatcaaata gacctgagaa agttttagtt agccactccg
     6241 ttagctgctc caccagttga gctactggta attttgtttg taatatcgtt tccaaaatgc
     6301 atcactcctt ttagttaagg tcttcgtctg agatattagc caaggcttca atcactcgtc
     6361 ctcgaataat gacacctaac agacgattat tatcatctgt cactgcaatc ggagcagatg
     6421 aatcatagat aagaggcaaa atatctgtaa taatagtatc ttttgagaca gttctcacat
     6481 cgcgatcaat cacttctgat agcggtaacc cttttttgcg cgcctctata gcggcatcgg
     6541 ccgtcaaact gccgactaat tggcggcggc gattcgtcgc catcaacata gacacctctt
     6601 cgttgtgcat acggttcaag gcaacttgag gtccatctaa ttcaacagta gttgttaacg
     6661 gtttgatcat gatattttgt gctgtcaaga ctttagaacg gtccacatcc tcaacgaatt
     6721 cacgcacaaa gtcattggct gggttagtca agatttcctc accagtacca atttgcataa
     6781 tttgtccgtc tttcatcaaa gcaatccgat caccaatccg caaggcttca ttcaagtcat
     6841 gactgataaa gatgatggtt tgtttcatgc tgtcttgcaa atcaagtaat tcatcttgca
     6901 tctcacgacg aatcaaagga tcaagcgctg agaatgcctc gtccattaag agaattttag
     6961 ggctattagc tagcgcacgg gctaggccga cacgctgctg catcccacca gatagttggt
     7021 ttgggtactg gtctttaaaa tctaataggc ctgaattatc aagggctttt tctgccaatc
     7081 gctggcgttc ttctttggga acgccacgta attccaaacc aaattcggta ttttccaaaa
     7141 tggttttatg aggaaagagg gcaaagcttt ggaaaaccat gttaatgtca tggcggcgca
     7201 cctcacgcag ctgatctgct gacatggttg agatgtcttt accttccagc aagatagatc
     7261 ctgctgaagg ttcaatcaaa cgatttagca tgcggacaag ggttgatttc ccacttcctg
     7321 atagccccat aataacaaag atttcacctt ttttgacctc aaaactagcg tcatagacac
     7381 ctacagtagc gcctgttttc ttaaaaatct cactcttatt tttgccagtc tttaccatct
     7441 caagagctgc tttttgtttt ttgccaaaaa ttttactgag atgtttgact tctaaaattg
     7501 tttccattcg ttttttctcc taggtaatcg tttatcatgt gaaataatga catagctttg
     7561 gcgaaatatt agccaatatt taacaaagta gctctacaca attaataaac gtgtggtcac
     7621 ttttttactt aaaaaaagaa tctgccgaaa cagaacataa ctatattatc acgtgtggtc
     7681 actttttgtc aaggacaata actcgatctg gtaatgccac aatgataggt gatcgctttc
     7741 attcttttag gtatggtctt ttaaccagta caatttttta tcacaactga tggctatttc
     7801 tttgctgatt aggctattgt tacattagaa atccttcttt tacctataat atctcttttg
     7861 cggccatcgt aaataacgac cacggtgact cttaaacatt tgctcatctc tccaaaaaaa
     7921 ctcacctaag acagtgtcct atgaagcgag ttagcttaat caggcactat catcttaggt
     7981 gagtagggtg ttgcttatag taaagcttta aattggatat tagaatcttc taagaagcag
     8041 tcatcaaatc cacgaggatg atggtattcg atacgatttt ggtcttgtgg ataggtgtac
     8101 ttgccaccaa cctcccagat aaagggatgg aaatcgtatt ggagacgttc tttgcgcatt
     8161 ttccacagtt caaggatttc atctgttgat gccataaagt ttgaccaaat gtcataatga
     8221 actgggataa cgactttagc acggagattt tctgccatac gaagaaggtc aacagaagtc
     8281 atcttgtctt gaattccaat tgggttttcc ccatagttat ttaacacaac gtcaatatca
     8341 taatcacgtc catgtttagc aaagtagttc gaaaaatgag aatccgcacc atgatagatg
     8401 gtaccacctg gtgtttcaaa aatatagtta acagctttgc gagccatgtc atcatctgtg
     8461 atagctaaac ctgctaaatc accatcttga gcatcagctc cttgtattgg gagagttacc
     8521 agacaggtgc ggtcaaacga ctctacagcg gtgatcttaa tatctttgaa ttcaaagcta
     8581 tcacctggct ttaagatcat aatacgatct tttggcacac cccaattttt ccaaacttca
     8641 ccacattcat aaggtcctac aaatttaaca tgattaagtt ttgggttatt gataatggct
     8701 gccgcagtat tgatatcgat gtggtcacta tgataatgag aaaccaaata ataatccaac
     8761 tcattaatca taaatggatc aatcaccatt ggttgggccc taagattggg ctgtaattta
     8821 cgagctcctg ccatattagc catttgatgt ccacgaacca tatccttaac ttgcttggtc
     8881 gctttcccac gatttgacca taagtccatg acaacattag caccgcctgg tgtcttaatc
     8941 cagataccac agtttcctaa ccaccacatc gcaaagttgt cagctggcac gacttcttgc
     9001 tcgatttctt catttaacca tgttccccat tctggaaacg tgttaaggat ccatgattca
     9061 cgtgtaatat cttgtacttt agtcattagg gtttcctccg aatttgttct tcatttctat
     9121 actttaagta tagactttct tgtcaatgat aaaaagacca agtgtcacac agatctgtgt
     9181 tcaaaagtgg actgactttt tcattagctc agtctaatac tgtctcattt tgcacactgt
     9241 atcttgcatc aatactgtat tcaaagctat attaaaaacc tcagttatat ttttctatgt
     9301 catagaaaac tgaggtactt tgacgactta acacttactc atagcagctc gcgatgaatg
     9361 agtttttcaa ttttagaaac caaggcataa cgatctgatt catcttttac aacagcttca
     9421 atagcttttg tgagctctgt agaaaagcgg gagtgctcac tcaagcgtcc ttgctttgaa
     9481 aagcgtatca atttgatgat atcatcgtca cttaaaatag ggttaacaat aagcatcggg
     9541 attttagtct ttagcgtatc tgtagtagca acaatcatat cgactgctaa aaggtcgtaa
     9601 acactctgat actgttctgt cgtaaaaaca gcttcaatct gaccatttgc tagatacctt
     9661 tgacactgtt tgaacaataa cttttgaatg cctatcccat catctgaaac aataacgagt
     9721 ttggtctttt cttgctctgt attattgtga cgcagctctc ccccaagatg gatagtcaag
     9781 taggcaatat catcgtctgt caagctaatc gtccaatctt gttccaaaat agtggcacaa
     9841 gattgagtca tagcaaacaa ttcttcgtat ttttctttaa tatggtctgt taagggatta
     9901 actaaaaaaa tcccataagc tttacggtaa acgagcgcct tacagtgggt cgtcaactgc
     9961 ttcagcaaat cctgtttatg cgtgaaatgt aactgataac ggctctccaa ctggtcaata
    10021 aagtgactaa tggttgctcg catgtcgtca taatcttgac tttccacgtg atgatcttga
    10081 tcttttcgaa aagacaacat caacatcgct accatactaa cttcgatatc atccaaatga
    10141 agcttaaaat tatggtaaag ctctctagct aaatcctgag caatgtgata ttctttacgt
    10201 ttccaaatca aatgaaattc ttgtttaaga gcagactttg tttctgaatc aagacgcata
    10261 ttacgataac ttaaaagcat gaaaggtaaa atctgcaaca tgaattggct atcttgggta
    10321 ttaattgtct ttcctaaact agcttgagat aaaaagagat actcatgaaa ataggtcaaa
    10381 atatctttag aaaaataaac cgataagcct tgtatctctg agagtttacg gttgaatata
    10441 tcaataaagc tagtattgcc accttggtaa acgtccacta acaatttgta caggtattga
    10501 ataagagcca ttggatgaca accaaaataa tatcccctag cctttgtcgc atgaagctga
    10561 atcttgtatt gtttgtcttc cagttcttcc cttaactcag tcaggtcatt taaaatcgtg
    10621 ttacgagaca catcattgat ctgcatcagt ttatcaatgg tcacgcgctc agtggaaacc
    10681 gcaatataaa tcgaggatag gcgtctgcgc tcatcacttt tcatgacata attataatca
    10741 gtcacttctt ctaataataa gcgacaagca gctttttgat cagctgttaa aagaatgcca
    10801 agtctaggat aagagataat ctgatctact ccctttggca aagcctgatt aattttgtca
    10861 agctggtaat aaactttacg acgtgactga tttaaagcat gagaaatggc cataactgtc
    10921 tcaggtgttt ctagtttaag taaataagac agcaagtcat aactcttttt atctaaaatc
    10981 atcatgttta caaacctcct ctctgaaaat ccatccttgc ttattttatc atgatttcag
    11041 tgactgcgca gccaagtttt gaaaaactca ccataataag attcgcttaa tatgattcga
    11101 gctaatcacc tcaactgatc caacaccaac accttatatc tggtctctaa gaactccaca
    11161 acatacgatt catccagttt gtgctcattg gcgtttaaat agttcatcga aaaggacaag
    11221 tcaaaaggac ttgcccaaac aatgattaac aaaaccttga actttctggc ttctagtgag
    11281 aacctgtgct aataagccat atcgtgacct catttttgac cgtaataagc atttgggcca
    11341 tgtttacgca aataatgctt atccataatg tatcgtggcg ctggctctac gcgtggattg
    11401 atttgttctg tcaagcgatt catcctagcc acttcttcta agacaaccga gtggtaaacg
    11461 gcttgctctg gggttttccc ccacgtaaat gggccatgat tgcgaaccac gattcctgga
    11521 accgccatag gatctaagcc tcgcttgcta aactcttcta aaatcacgtt gccagtttct
    11581 tgctcgtaag ccccatcaac ttctgctttt gtgagagaac gagcacacgg aactggtcca
    11641 tagaagtagt cagcatgcgt agtcccataa aacggaatat cacgacctgc ctgagcccaa
    11701 ccaacagctt ctgtggagtg agtgtggaca ataccgccca cttctggcca agctttataa
    11761 agttccacat gggttggcag atctgaagaa gggttcaaat caccttcgac cacattccca
    11821 tcaaggtctg taacaaccat gttttctggt gtcaagaggt cgtagtccac cccagaaggc
    11881 ttgatgacaa tacgtcctaa ttcacgacaa acttcagaga cattgcccca cgtaaatttg
    11941 acaagaccgt gttgtggtag agacttgtta gccgcacaga cacgttcacg catttcttgc
    12001 aagtttttcg ccatattatg ccaaccccgc tttctcaata agtgggtaaa ggaagtcctg
    12061 agcttctttg atagcagctt ttgtttcctc caccgtgtca caattttctg accacatttc
    12121 aatcaaaaaa ggcccattgt agttggtttt cttgaggacc gcaaacagct cttgccagtc
    12181 cacacaacct tgaccaaatg gcacatcacg aaactgccct tttgaggttt ccgtgacagc
    12241 ataagtatct ttcaagtgga gggctgcaat ggacttgtga ccattgtaaa attcactcca
    12301 caaatcatta tgccaggcag aaacattgcc tgcatcagga taaacaaaga gataaggcga
    12361 gtcaatctct ttttcaactg ccaagtattt ttcaatagag ttgataaaag ggtcatccat
    12421 aatctcaata gaaagcatca cttgggcttc ttcggcccag tcacaagatt gtctcaagtt
    12481 tttgataaaa cgagcacggg tttcaggcga tttttcttca tagtaaacgt cataaccagc
    12541 taattgaatg gtacgaacgc ctaagtcctg tgccaattca atacattgtt tcatcagttt
    12601 aagggacttg gcttcaatag caggatcatt tgagcctagt gggtaacgtc tatgaccaga
    12661 aaaacaaatg gtcgggatgc gaatgcctgt ttcataaatg gctttcacta aatcaagccg
    12721 ttcttcctta gtccactcta agcgcgctaa gcgagcatct gattcatcaa cagacatttc
    12781 cacaaaatca aaacctaagt cttttgcaaa ttgcaagcgt tcacgccagg taaactgttt
    12841 tggcgttgct ttttcataaa taccaatagg acgcgccata aagttacccc cagatacgtt
    12901 tgatttcatc tttgaaggca cgcgccgctg cagcaggatc ttctgcttca gtaatgcctc
    12961 gccctgcgat aaaggtaaag acatcaaccc cttcaaaaag tttcaaagtg tctacatcaa
    13021 gcccaccagt tactgacacg cggaagccca tgtcaatcaa tgttttcacc ttgttaaggt
    13081 ctttttcacc ccaagtttct ccagcaagaa gggcatcacg tgattggtgg taaatggctt
    13141 gggaaatccc agcgtcaagc cataattgag cttgctcata agtccaatca ccataaagtt
    13201 cgatttgaat ttccccacgg tcaccgcgct cttctttcat agccttgagg gctgcttcca
    13261 tggttggaat cgtcgcacag caaatgcagg tcatccagtc agcaccacgt tttgcattgt
    13321 ttttagcgac cgtaccgcca gcgtcagcac atttggtatc tgctacgata attttctctg
    13381 ggaagaggct gcgtaatacc tcaaccaact cgcttccgac ttgaagcaag cagaccgtac
    13441 cggcttcgat aacgtctact tcgtgaccca cagcaacggc tgctttaaca gccccctgta
    13501 agtcagaatg gtctaaagca acttgtaaat ttggaatatg tgtcatctct ttttcctaaa
    13561 actcttagcc accgtgcaaa ctggttaaga gttcttatcc tttccatcat tcgttagtat
    13621 cctgtgtaag gaaagcttaa gcattcaagt ccataccttc taagtaagga ctatctttag
    13681 attcttctac catttctaga acctctttag gactttggca ggccaccaag cgttcaatgg
    13741 cgttatctag ttcaaacaag gccacaattt gtgggatagc cactgttgtg tgaatagaag
    13801 ggtctgtagc ggccaacgtc aaaagaacag aaacctcttt accatctgag aaggtaactg
    13861 gttttgttag tgtaaccaaa gcaaatgcat tgcgattaac gcctaaacct gcttctgcat
    13921 ggggcatagc catacctggc atcaacacat aataagggcc gtatttttct gttgatgcaa
    13981 tgatggcgtc gtagtaagct gacgtcactg ctttgctatc aatcaaaggt tgaacagcta
    14041 attgaacagc ctcttgccaa gtatcagcac ttagccccaa tcggatggag ttgttgtcaa
    14101 taaaggcttg ttttaaattc ataaaaacta tctctttcta gtgtcttgct ttgcaacaca
    14161 caccattttt actagtttta tcctactaaa gagaaagagc tgaaaacctt cgtcacaact
    14221 ccttcgtttt gattcagaat tagcttatag cacttcttgt agtttggttt taatttcatt
    14281 atcatccatc aggttatcaa gaccaaccaa gtggcctttg gtacgaccat caagctcatg
    14341 aatcaagtga tttgatgcca ctacaatatc atagccagaa gctagacctt tagcttcacc
    14401 aactgagcaa gaagctgact gaatatctgt cacgccaagt tgacgaagcg cattttcaac
    14461 tttcattttg atcaccatag atgaccccat accattgcca catgctgtta agactttaac
    14521 cattgttttt tcctccgaaa atgttttatt attgttttgt taaaatagca gagctatttc
    14581 ttaagatgca gtgttattga gcttccccac ggtaataagc ttctttgtcc ttagcttttg
    14641 cgaattgcaa ttgtggaatg gcaagtaaga agatgcaaac aagcacatac cctgcaatac
    14701 caaggaattt gaagaggtac ccaaatggta accatggaag aaccaagtcg atattaccgt
    14761 gataaccacc tgtaagtcct aaaagaccta cagcaacagc accaagtgca acttgcaaaa
    14821 tacctgaaat aaatgataga gctaccgctg ctttccagcc accacgttta tctgcataaa
    14881 ccgcaattgc cgcattatca aagaatacgg gaacgaatcc tgtgataatt aagattggat
    14941 ttttaaagat aacaagaaga gcaatggtga ttaattgacc aatcaaacca aaggcaaatc
    15001 ctgaaagcac cgcatttgaa gaaccaaagc cataggaagc tgcaacgtca accgctggga
    15061 atgaccctgg aagcaatttg cttgagatcc cttgaaaggc attggtcaat tcagaaacaa
    15121 acatgcgaac accctgcatc aagataaaga gataaactga gaaggtgaga gaggtttgca
    15181 aaatatacat gaagaaagct tgcttagctg ggttgaatgc tccaggtccg attaagtcca
    15241 catcagacat aatgtctgga ccaaggacag ccaagatagc tccgaaaaag accaacatca
    15301 aagttgctga cgcaacaaca gtatcatgga aaatgtttaa gaaggttggt aattttaggt
    15361 tatcaaggtt ttcttctttt ttaccaaaga acggtgcaac cttatccaca aaccaaatcg
    15421 caaattgttg ttggtgacca atcgcaaacc caccgccacc tgtaaggcgt tgagttgctt
    15481 caacggtcat gtttgagctg atagcccagt aaagcccaca gatgataccg actgcccatg
    15541 ccccaaaagc attttggaat tgagggatta aaagaaggac aaagaccgaa atcgtcgctg
    15601 cctgttggac catgatgtga cctgtgatga aaagggtacg aactttagtg accttgcgaa
    15661 gtgcaaccaa aagaatgttg accccaaatc caatcaagag agctgtcgta gcaacactga
    15721 tgaagcccat ctcttctaac ttagtattag cagcagccaa accaaaataa ggatcaataa
    15781 cggcggcttt caattcaaat ttcttggcaa gagcaaccaa gatcggacga aaagtcgtta
    15841 caagaccgcc tgctccaacg ttcaaaatca agtaaccaac ggtagccttt acaaaaccag
    15901 caaagacctc ataaatgggt tttttaagga gtaaataccc gattaaaacc aaaagaccta
    15961 caaagaacgc tgggttttgc aaaatatttt gcgagaacca gtttagggga gctaggagca
    16021 tttccataag aactacctct tttcaaagtt aataaagata aagtgtttat atgttcatta
    16081 taggtaatga aagcgtttta aaaaagacca actatcacac aaagaagtgt tcaatattag
    16141 acttaagtta acttatttta aaatttaact acacttaata ataaggagaa aatcacccta
    16201 aacaacgatt aaaaaccaca agaaccttcg ttcttgtggt ttttctagat gagttgcttt
    16261 ttgaatagat cagctctttg gcattaactg tgacgtgacg caaataacca ctgaagggct
    16321 gtgtcacctc aaactatttg ataacaatat taacgagttt gtttggtact gcgatgactt
    16381 tgatgatgtc tttgcctgcg atttcggctt ggactttttc gttagcaagg gcaacttctt
    16441 gtaattcttc gcgtgaaagg tctttagcaa ctacaagttt agctttgacc ttacctttga
    16501 tttggacaac gatttccaca tcgttttcaa ccaatttgct ttcatcataa cttggccatg
    16561 gcacgtaaga gattgactca cctgaggctg tcaaggcttg ccagagttcc tcacctaagt
    16621 gcggtgcaaa tggtgcaatc aattgaacaa agcctttggc atagtcgcta aagagtttgt
    16681 cttccttgtt ggctgcattg acaaatacca tgagttgagc gatagctgtg ttgaatttca
    16741 tttggtcaac ttgctcagta acggccttaa cggtttcgtt gtaaaccttg tcaagggctc
    16801 cgctattttc ctcagtgatc tctttagtcg tgatcaaacg gtaaacacgg tccaagaact
    16861 tacgtgagcc ttcaagacct tcttcagacc aagcgatgga agcgtcaagt ggtcccatga
    16921 acatctcgta aacacgaagg gtgtcagcac cgtactgctc aacaacatca tctgggttga
    16981 cgacgttctt aagtgattta gacatcttag ctggtgcttg ttcaagctct tccccagttt
    17041 ccacatggaa gaagctgcca tcacgttttt caaccttgtc agtcgctaca agcgctccac
    17101 ggctatcgcg gtagcttgtc cccaaaatca tcccttggtt aaagagtttt tggaatggct
    17161 ctttggtagg tacaacaccg agatcgtaaa gaaccttgtg ccagaagcga gcgtagagaa
    17221 ggtgaagcac ggcatgctct gcaccaccga catagatatc aactggcaac cattgtttga
    17281 ggagttcctc atcagccagt ttttcggtgt tgtgtggatc gatgtaacga agataatacc
    17341 agcttgaacc tgcccattgt ggcatggtgt tagtctcacg acgaccttta acaccatctt
    17401 cacgggtaac ttccaaccag tctgtcacgt tagcaagcgg gctctcacca gtacctgaag
    17461 gacgaatatc cttagttact ggaagaacca atggcaattc actctcagga accgctgttg
    17521 atgtcccatc ttcccagtgg atgattggga ttggctcacc ccagtaacgt tgacggctaa
    17581 agagccagtc acggagacgg taagtgacat tttcattacc gacgccttct gcttcaagcc
    17641 attcgaccat tttggcaata gctgacgctt tgtcaagacc atctaggaag tcggagttga
    17701 tgtgaagacc gtcttctgta aaggcagcct cttcaacatt accaccttca agaacaggga
    17761 tgatgtctag gttaaattgt tttgcgaatt cccagtcacg ttcatcgtgg gctggaacag
    17821 ccatgatagc accagtaccg tagctagcaa ggacatagtc cgcaatccaa actggcattt
    17881 ctttgccgtt gaccgggtta atcgcatagc tacccgtcca gacaccagtt ttttctttgg
    17941 caaggtctgt acgagcaagg tctgatttga gacttgcttg acgtttgtag tcagccacgg
    18001 cttctgcctg gtcagatgtc gtaatagcat caaccaaggc atgctcagga gcaagaacgg
    18061 cgtaagttgc accaaaaagc gtgtcaggac gtgttgtaaa gacagtgaag tccttatctg
    18121 tatccttaac cttgaaggtt acattagcgc cagttgattt accaatccag ttgcgttgca
    18181 tgtccttgat tgattctggc cagtcaactt cttccaagtc ttctagcaag cgctctgcat
    18241 aggcagtgat tttgagcatc cattggcgca tgggttttct gacaactgga tagccgccac
    18301 gttctgatgt cccatctgga agaacctctt cgttggcgat agctgttccc aattcttcaa
    18361 cccagttaac gggaacttca gcttcgtagg ctagaccttt ttcataaagc ttggtaaaga
    18421 tccactgcgt ccacttgtag tagttagggt cagttgtgtt aacttcacgg tcccagtcgt
    18481 atgagaagcc aagagcatta atctggcgtt tgaagttagc gatgttttca gctgtaaact
    18541 cggctggatc attaccagta tccatagcgt actgctcagc aggaagtcca aaggcatccc
    18601 aacccattgg gtggaggaca ttgtgacctt gggcacgttt gaaacggcta agaatatcag
    18661 ttgctgtata gccttctggg tgaccaacgt ggagaccagc tcctgatgga tatgggaaca
    18721 tatctagggc ataaaactta ggttttgatg cgtctgttcc tgttttaaag gtgtgattat
    18781 ctgcccaaaa ggcttgccat ttgggttcaa tagcggtgtg atcgtaaaat gtcatgtgaa
    18841 atctcccatg taaaaaatct agtcttctta ttatagcatg agatgaagct tttaggaatg
    18901 gtcaagacat gactagcacc ttggcttttg agacttcaga cgattagcta tttttaaacc
    18961 ctgctttagc atcaaccatc ctcacaaaaa gctaataaaa aagccagact tgctctgtaa
    19021 acagagccaa tctggacaaa tagacttaaa aagcaagtgc ttgctcaaaa tcggccaata
    19081 agtcttcgat attttcaaca ccacaagaca agcgcagcaa accatcaaca accccttgtt
    19141 ccaaccgcgc ttgtgacgat aaacaagcat gagacatcgt tgctggatgt gacaaaatgg
    19201 actctactcc acctagacta acggctaaaa tcggcaactg aatacgatgg gtaaaggaca
    19261 tcagctcctc cttagaagcc aactcaaagg ataaaacagc accaccattc ttagcttgtt
    19321 gcaggtgggt atcgtgatta ggatgactgt ctaagccagg gtaatgtacc ttaagaactt
    19381 tggggtgctt cgctaaatag ttagcgagct gctgagcatt ttttaccgct tgttccatac
    19441 ggatccccat agttttcatg ccacgaagaa tcagccaagc gtcttcgacc ccaacaattg
    19501 ctccaaagtt tttttgaaaa agcttgagct ggttgtagat agcctcatta ttggtagcag
    19561 ctaaaccagc taccacatca ctatgaccat tgataaattt ggtcacactt tctactacaa
    19621 tatccacacc catagctaag ggttcttgat acaaactggt cataaaggta ttatcagcca
    19681 cagttagcac accataagct ttggcgagtt gcgcaagctc tgaaatatca caaattttca
    19741 agagcggatt tgacggtgtc tctagataaa tcatacgcgt attaggcctg atacttgctt
    19801 ttactgttgc taaatcagcc atatctacaa agcttgtttc aatctgataa ttgggtaaaa
    19861 tagctgtcgc aaattgacaa gtccctccat agacctccaa tgggaaaatc acatgatctc
    19921 cagcttttaa gagcatcaaa acagttgaaa tagcagccat accagatgca taagccaccg
    19981 cataagtcgc tttttctaaa caggctaacc cgtcttctaa agcttcagtc gttgggtttg
    20041 aaaagcgtgt gtacaaatgt ttttgactag gacaatacag ttctgaattg tggaacgtcg
    20101 aagtctgata gatgggaact gaagctgctc ctgtaaactc gtcaataacc gtatagccat
    20161 gtaataaggt cgtattttct ctcatttcgt gcacctccgc aatagcgtta actccaatga
    20221 actctctttt agacaatggt cccaccccaa tggttgcaca aaccaaaaag ctcttcaaaa
    20281 aagcgtgatc tgctaaccta taagaaaaat aaagaaaagt agtgggcacc ttttaaatgg
    20341 ataatgaatt tttccaataa tatcattttt agatcgatta cgtttattat acaaccttta
    20401 gctaagtttg caagcccttt tatcatcttt gaccaagctg ccaacacctc tccagcaagt
    20461 taaagtatag ctacctttgg ccccatcgtc tcaaaagctc aaattatcac actgacagtc
    20521 ctaccttgca tcgtcaccta ctcaatatgg caatactgcc atcaaaattt cattaaaagg
    20581 tcacaattta tttcatttaa tgagaaacag aacttgtttt caccatgcgt cttatagtat
    20641 accgctaaaa tactatttat ttttaggaga gaaatatagt gaaaaacaaa ttatttttag
    20701 ttgcccttgc gaccgtaact gtcctagggc cctctttagc aacccctcat catcagaccg
    20761 tgcatgcggc tgagcaggca aaagaacccc aaaccagctc aatgacgtgt actaacgatg
    20821 gatctgtatg cgctggctgg catagtttat cagaaggaga cagtataact tcaaattcat
    20881 cttcaaacac caagacttcc ctgttaaact ggttatggag cttaataaca aatttgtgga
    20941 attctgcaaa aaacttgttc atctaagatc tctataatca tatccatgga atttaaataa
    21001 ccatctcgtg gcggttttct aatcctaggt cgccacactt cttattcaga taaataataa
    21061 taaaaacatt aaaataacac tttatttatt aacaaaaagt tagtataata taatcacaat
    21121 acaaattaaa ggagttataa atgaaaaaga aattatcttt atttatgatc gcaactgctg
    21181 ctgtcgtaag ccttagcctt gcaacactag gaagccacca aactgtttca gcaaatgatg
    21241 ctagctatag taacggtttc tacggacaca ctggtcctga cagctggtta ctttacacag
    21301 tatggtaaac ctaataccgt aacatagaag ctatcttctt cgtaagacta cggttatgaa
    21361 aagcctagag ctgcgtaagc agttctaggc tttttgattg gctaaaacgt ttttactaac
    21421 gatccctcaa gattaaaaag aaaaggcata ccgcctataa aaggacaagc ccaaaacaca
    21481 agtcgtaaca gttgctagct aaccaccacc aaaaaagctg taacacctca aatataatta
    21541 taaccagatt aacattatta ataattaatg ttatttatat aagataaaga aatgctttag
    21601 tatcggcttt ctttgccttt ttcggtcaaa cacataacaa aaagattaaa atgtgatatt
    21661 gaatcattaa cataatatga gtataatata ggtatactat aatttaagga gtta
//
LOCUS       DAXWIQ010000030        21146 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000030 DAXWIQ010000000
VERSION     DAXWIQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21146
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.030"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(527..913)
                     /gene="rplQ"
                     /locus_tag="ACGEUI_001165"
     CDS             complement(527..913)
                     /gene="rplQ"
                     /locus_tag="ACGEUI_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFF8166005.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(928..1866)
                     /locus_tag="ACGEUI_001166"
     CDS             complement(928..1866)
                     /locus_tag="ACGEUI_001166"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFF8166006.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELEVEGP
                     AEVTAGDILTDSDIELVNPDHYLFTIAEGHSLRATMTVAKKRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEITTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKSEPEMMKVRNLGRKSLEEVKVKLADLGLGLKNDK"
     gene            complement(1912..2295)
                     /gene="rpsK"
                     /locus_tag="ACGEUI_001167"
     CDS             complement(1912..2295)
                     /gene="rpsK"
                     /locus_tag="ACGEUI_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFF8166007.1"
                     /translation="MAKPTRKRRVKKDIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2313..2678)
                     /gene="rpsM"
                     /locus_tag="ACGEUI_001168"
     CDS             complement(2313..2678)
                     /gene="rpsM"
                     /locus_tag="ACGEUI_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFF8166008.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDIR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNMNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2696..2812)
                     /gene="rpmJ"
                     /locus_tag="ACGEUI_001169"
     CDS             complement(2696..2812)
                     /gene="rpmJ"
                     /locus_tag="ACGEUI_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFF8166009.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2838..3056)
                     /gene="infA"
                     /locus_tag="ACGEUI_001170"
     CDS             complement(2838..3056)
                     /gene="infA"
                     /locus_tag="ACGEUI_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFF8166010.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(3174..3809)
                     /locus_tag="ACGEUI_001171"
     CDS             complement(3174..3809)
                     /locus_tag="ACGEUI_001171"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFF8166011.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGIAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPSCLVERLSGRIINRKTGETFHKVFNPPVDYKEDYYQREDDKP
                     ETVKRRLDVNMAQGEPILEHYRKLGLVTDIEGNQEITDVFADVEKALLELK"
     gene            complement(3959..5263)
                     /gene="secY"
                     /locus_tag="ACGEUI_001172"
     CDS             complement(3959..5263)
                     /gene="secY"
                     /locus_tag="ACGEUI_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFF8166012.1"
                     /translation="MFLKILKDALKIKTVRNKIFFTIFIILVFRIGTHITVPGVNAKS
                     LEQLSELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFAQSIGITAGFNTLSNVALVKTPDIKTYLLIGALLTTG
                     SVIVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAIATIREDYFVNVKASDLHSSYLI
                     VGILIIAVLAIVFFTTYVQQAEYKIPIQYTKLMQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFVQNGRDLPWLNRLQEIFNYQTPVGMIVYALLIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSVRPGRETEQFMSALLKKLATVGAIFLAFISLAPIAAQQALNL
                     SSSIALGGTSLLILISTGIEGMKQLEGYLLKRKYVGFMNTAE"
     gene            complement(5280..5720)
                     /gene="rplO"
                     /locus_tag="ACGEUI_001173"
     CDS             complement(5280..5720)
                     /gene="rplO"
                     /locus_tag="ACGEUI_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFF8166013.1"
                     /translation="MKLHELKAAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRIPKRGFTNINTKEYALVNLDQLNVFDDGTEVTPAILKDAGIV
                     RAEKSGVKVLGNGELTKKLTVKAAKFSKSAEAAIIAKGGSIEVI"
     gene            complement(5934..6116)
                     /gene="rpmD"
                     /locus_tag="ACGEUI_001174"
     CDS             complement(5934..6116)
                     /gene="rpmD"
                     /locus_tag="ACGEUI_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFF8166014.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVTAISHLVTVEDVK"
     gene            complement(6131..6625)
                     /gene="rpsE"
                     /locus_tag="ACGEUI_001175"
     CDS             complement(6131..6625)
                     /gene="rpsE"
                     /locus_tag="ACGEUI_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFF8166015.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDGNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMIEVPMVGTTIPHEVYTNFGGAKVLLKPAVEGS
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6644..7000)
                     /gene="rplR"
                     /locus_tag="ACGEUI_001176"
     CDS             complement(6644..7000)
                     /gene="rplR"
                     /locus_tag="ACGEUI_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFF8166016.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKDVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(7105..7641)
                     /gene="rplF"
                     /locus_tag="ACGEUI_001177"
     CDS             complement(7105..7641)
                     /gene="rplF"
                     /locus_tag="ACGEUI_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFF8166017.1"
                     /translation="MSRIGNKVITMPAGVELTNNNNVITVKGPKGELTREFNKNIEIK
                     VEGTEITVVRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKDLEMKGVGYRAQLQGTK
                     LVLSVGKSHQDEVEAPEGITFTVANPTSISVEGINKEVVGQTAAYIRSLRSPEPYKGK
                     GIRYVGEYVRLKEGKTGK"
     gene            complement(7844..8242)
                     /gene="rpsH"
                     /locus_tag="ACGEUI_001178"
     CDS             complement(7844..8242)
                     /gene="rpsH"
                     /locus_tag="ACGEUI_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFF8166018.1"
                     /translation="MVMTDPIADFLTRIRNANQVKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGKNGERVITNLKRISKPGLRVYAKRDDMPKVLNGLG
                     IAIISTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(8393..8578)
                     /locus_tag="ACGEUI_001179"
     CDS             complement(8393..8578)
                     /locus_tag="ACGEUI_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFF8166019.1"
                     /translation="MAKKSMIAKNKRPAKHSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RELAYKGQIPGVVKASW"
     gene            complement(8594..9136)
                     /gene="rplE"
                     /locus_tag="ACGEUI_001180"
     CDS             complement(8594..9136)
                     /gene="rplE"
                     /locus_tag="ACGEUI_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFF8166020.1"
                     /translation="MANRLKEKYTNEVIPALTEKFNYTSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEISFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(9160..9465)
                     /gene="rplX"
                     /locus_tag="ACGEUI_001181"
     CDS             complement(9160..9465)
                     /gene="rplX"
                     /locus_tag="ACGEUI_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFF8166021.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVIVEGVGMIKKH
                     QKPNTENPQGAIVEKEAPIHVSNVQVLDKNGVAGRIGYKVVDGKKVRYSKKSGEVLD"
     gene            complement(9544..9912)
                     /gene="rplN"
                     /locus_tag="ACGEUI_001182"
     CDS             complement(9544..9912)
                     /gene="rplN"
                     /locus_tag="ACGEUI_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFF8166022.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9937..10197)
                     /gene="rpsQ"
                     /locus_tag="ACGEUI_001183"
     CDS             complement(9937..10197)
                     /gene="rpsQ"
                     /locus_tag="ACGEUI_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFF8166023.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(10223..10429)
                     /gene="rpmC"
                     /locus_tag="ACGEUI_001184"
     CDS             complement(10223..10429)
                     /gene="rpmC"
                     /locus_tag="ACGEUI_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFF8166024.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(10439..10852)
                     /gene="rplP"
                     /locus_tag="ACGEUI_001185"
     CDS             complement(10439..10852)
                     /gene="rplP"
                     /locus_tag="ACGEUI_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFF8166025.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10856..11509)
                     /gene="rpsC"
                     /locus_tag="ACGEUI_001186"
     CDS             complement(10856..11509)
                     /gene="rpsC"
                     /locus_tag="ACGEUI_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFF8166026.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11522..11866)
                     /gene="rplV"
                     /locus_tag="ACGEUI_001187"
     CDS             complement(11522..11866)
                     /gene="rplV"
                     /locus_tag="ACGEUI_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFF8166027.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKKVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11882..12160)
                     /gene="rpsS"
                     /locus_tag="ACGEUI_001188"
     CDS             complement(11882..12160)
                     /gene="rpsS"
                     /locus_tag="ACGEUI_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFF8166028.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(12299..13132)
                     /gene="rplB"
                     /locus_tag="ACGEUI_001189"
     CDS             complement(12299..13132)
                     /gene="rplB"
                     /locus_tag="ACGEUI_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFF8166029.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVSLKSKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPDADIKVGNALPLANIPVGTVVHNIELKPGKGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(13150..13446)
                     /locus_tag="ACGEUI_001190"
     CDS             complement(13150..13446)
                     /locus_tag="ACGEUI_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFF8166030.1"
                     /translation="MNLYDVIKKPVITEKSMIALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVNVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(13446..14069)
                     /gene="rplD"
                     /locus_tag="ACGEUI_001191"
     CDS             complement(13446..14069)
                     /gene="rplD"
                     /locus_tag="ACGEUI_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFF8166031.1"
                     /translation="MANVKLFDQTGKEVSSVELNDAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVEGLSFAAPKTAEFAKVLSALSIDTKVLVLVEEGN
                     EFAALSARNLPNVTVATAATASVLDIVNADKLLVTKEAISTIEEVLA"
     gene            complement(14093..14719)
                     /gene="rplC"
                     /locus_tag="ACGEUI_001192"
     CDS             complement(14093..14719)
                     /gene="rplC"
                     /locus_tag="ACGEUI_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFF8166032.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVILVKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14935..15243)
                     /gene="rpsJ"
                     /locus_tag="ACGEUI_001193"
     CDS             complement(14935..15243)
                     /gene="rpsJ"
                     /locus_tag="ACGEUI_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFF8166033.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(15444..16736)
                     /locus_tag="ACGEUI_001194"
     CDS             complement(15444..16736)
                     /locus_tag="ACGEUI_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFF8166034.1"
                     /translation="MIYNNRRKIFSLALPSMIENILQMLMGMVDNYLVAQIGLVAVSG
                     VSIANNIISIYQSLFIALGAAVSSLIARSIGENNQNKQLNYMAGVLQVTLLLSVGLGL
                     LSVAGHHQVLEWLGAEASVTLVGGQYLSIVGGMIVSLGLLTSLGAIVRAQGYPKIPMQ
                     VSLLINVLNAIFSALSIYVWGFGLLGVAWATVLSRLVGVFLLCQFIPIKQVAKRLMRP
                     LDKIIFDLSLPAAGERLMMRAGDVLIIGIVVRFGTTALAGNAIGETLTQFNYMPGLAM
                     ATATIILVARQLGGGKVTEIRYIIREAFILSTLMMLVMGALTYLLGPSLLPLFTQNTD
                     AQRSAMIVLLFSLLGAPATAGTLVYTAVWQGLGKAKLPFYATTIGMWVIRIGLGYVIG
                     VVWQYGLIGVWMATVLDNTSRWFILSKHFKKYQEITFH"
     gene            complement(17124..18140)
                     /gene="adhP"
                     /locus_tag="ACGEUI_001195"
     CDS             complement(17124..18140)
                     /gene="adhP"
                     /locus_tag="ACGEUI_001195"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFF8166035.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPNIGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLKVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAVVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIVIFGAGGLGNLAVQYAKKVFNAHVVAVDINNDKLELAKEVGADILVNGKEIE
                     DVPGYIQEKTGGAHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIKVVGSLVGTRKDLEEAFAFGAEGLVAPVVEKVPVDTAPEVFDEMERGLIQGRKVL
                     DFIS"
     gene            complement(18392..21034)
                     /gene="adhE"
                     /locus_tag="ACGEUI_001196"
                     /gene_synonym="adhC"
     CDS             complement(18392..21034)
                     /gene="adhE"
                     /locus_tag="ACGEUI_001196"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFF8166036.1"
                     /translation="MTEGHNTVETTSVSVTIDALVQKGLAALEEMRKLDQEQVDYIVA
                     KASVAALDAHGELAKHAYEETGRGVFEDKATKNLFACEHVVNNMRHQKTVGIIEEDDV
                     TGLTLIAEPVGVICGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWVETPSLEATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDDFVAEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKPATWIAEQAGFTVPEGTN
                     ILAAECKEVSENEPLTREKLSPVIAVLKSESREDGVEKARQMVEFNGLGHSAAIHTAD
                     AELAKEFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNAVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTELMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVLTVPGFIAADTGMDVLTHATEAYVSQMANDF
                     TDGLALQAIKIVFDNLEKSVKTADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPASTP
                     EEAVESYAKAVYDLGCRVGIQMNFKAQGIDENEWKEHSRELAYLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYAERPGRRK"
BASE COUNT         6092 a         4471 c         3817 g         6766 t
ORIGIN      
        1 aattttgttg gaaacggctt aaagtgacag attttaaaag taaaaaagat attttcacct
       61 ccaaattaaa agaggatttt agaatactta ttaataaaat attgaatagg ggataaaact
      121 aacaatatag tttgagcatc tgtttgcgat gcaagttttt ttcggacatt taaacgaggg
      181 gattacaccg tttatttgcc catgagaggt ctttaaacac ctgcataagg ttttcttatt
      241 ctgatgacct tattgtctct gagaaaactt tataaacgca tcagaagagg cttctgtgtt
      301 attgttttgc tcagcttaaa agcggggggt gttcacctca taatacaaca aaacgcctgc
      361 ttataaaaag tttctatagc ttagatacct ttcataaaca agcgttacat attaacaata
      421 tgatgagtgt tcccgctagg aaaaatccta gcggtagacc agagctagac taagaataat
      481 ctctcgatta ctccatcatc ataacactca acaaaattga taaaaattaa actaattcaa
      541 taattgccat tggagcagca tctccacggc gtggttctgt tttaagaata cgagtgtatc
      601 caccgttacg ttctgcataa cgaggtgcaa tttctgagaa aagtttttga agagctgtcg
      661 ttgatgtgta tttatcggta gcttcatcat aattttctga tgcgatttcg ttacgaacgt
      721 atgcagctgc ttggcgacga gcatgaagat caccacgttt accaagagta atcatttttt
      781 caactgtttt acggatttct tttgcacgtg cttctgtcgt tacaattgat tcgttgatca
      841 aaagatctgt cgtcaaatca cgaagcattg ctttacgttg tgagctagtg cgtcctagtt
      901 tacggtaagc cattttgtcc tcctatatta tttatcgttt tttagtccga gacctaagtc
      961 agcaagttta accttaactt cttcaaggct cttacgtcca agattacgga ctttcatcat
     1021 ttcaggctca gatttttctg ttaaatcgaa gacagtgtta atgcctgcac gttttaaaca
     1081 gttatatgag cgtacggata aatcaagctc ctcgatggtg cggtcaagta ccttttcatc
     1141 gttcactttt tcagtttctt tcattacttc ggtagctttt gcaacctctg ttaaatcagt
     1201 aaacaggttt aagtgttcga tcaaaactcg agcagataga cctaaagcat cttcaggaat
     1261 aattgttccg tttgtcgtga tttcaatcgt taatttatca aagccatcat tgctacctac
     1321 acgggcaggt tcaacttgat aattaacttt tttaactggc gtatagattg aatctactgc
     1381 caatgtaccc acaggtgcat catctttttt gttgccttct gctggcacat aaccacgttt
     1441 tttagcaaca gtcattgttg cacgtagaga atgtccttca gcgattgtaa aaagataatg
     1501 atccgggtta acaagttcga tatcactgtc agtaagaata tcaccagctg ttacctctgc
     1561 tggtccttct acttcaagtt cgataatctt ttcgtcttcg acgtatgatt tcacagcaag
     1621 tcccttaaca ttaaggataa tttgcatgac atcttcacgt acacctggga ttgtatcaaa
     1681 ttcgtgtagt actccatcaa ttttaattga tgttactgct gcacctggaa gtgaagacaa
     1741 gagtacacga cgaagagaat tacctagagt tgttccgtag ccacgttcaa gtggttcgat
     1801 gacaaatcta ccgtaatctt tattttcatc aatttttgtt attattggtt tttcaaactc
     1861 aatcattttt tcacccctcg aaacgaaact tgtgtactat ttgttatatg attatacacg
     1921 acgacgtttt ggaggacgag caccattatg tggcacaggg gtcacatcac gaattgcagt
     1981 tacttcaaga cctgcagctg ctagagcacg aatagctgat tcacgacctg aaccaggacc
     2041 ttttacagtc acttcaacag tttttagtcc gtgttcttgt gcagattttg cagcagcttc
     2101 tgcagccatt tgagcagcaa atggagtaga tttacgagaa cctttgaaac caagagcacc
     2161 agctgatgac catgcaagag cattaccatg cacatctgta atcataacaa tagtgttatt
     2221 aaatgtagcg tgaatgtgtg caacaccaga ttcgatgtcc tttttcacac gacgtttacg
     2281 tgttggttta gccaattgtt ttacctccta ttttattttt tcttacctgc aatcgcaaca
     2341 gctttccctt tacgagtgcg agcattgttt ttagtatttt gtccacggac aggaagtcca
     2401 cgacgatgac ggattccacg gtatgaaccg atttccatca aacgtttaat gttcatgttt
     2461 acttcacgtc gaaggtcacc ttcaactttg attgcatcca cttcacgacg gatagcatct
     2521 tcttgatctg atgttaaatc tttaacacgg atatcttcag aaatacctgc agctgctaag
     2581 atttttttag atgttgcaag accaattccg taaacataag taagtgaaat tactacgcgt
     2641 ttatcatttg gaatatcaac tccagcaata cgagccattt tttctccttt ctattttatc
     2701 cttgacgttg tttgtgtttt ggatttgtcg gacaaattac cataacacga ccattacggc
     2761 gaataacttt acagtattcg caaattggtt taaccgatgg tcttaccttc atgttttaat
     2821 ccctccaatt atttcgatta tttaaagcgg tatgtgatgc gtccacgtgt caagtcataa
     2881 ggactcattt ctactgtaac acggtcacct actaaaatac gaatgtaatt tttgcggatt
     2941 tttcctgata cagttgctag aatttgatgt ccattttcca attcaacagt aaacattgca
     3001 tttggcatcg tttctactac tttgccttca atttcaatca cgtcttcttt tgccacgcaa
     3061 aagtaccccc taaatttcga tttgatgtcc tctgagcaca gaggtaacaa ttataagtca
     3121 gactaaacta gtatatcatt actaatctac ttatgcaagt agatttgtat aatttatttg
     3181 agttctagca acgctttttc aacatctgca aaaacgtctg ttatttcttg attaccttca
     3241 atatctgtaa caagaccaag cttacgatag tgttcaagaa taggttctcc ttgagccata
     3301 ttgacgtcca aacgacgttt aacagtttca ggcttatcat cttcacgttg atagtaatct
     3361 tctttataat ctactggtgg gttgaacact ttatggaacg tttcaccagt tttacgattg
     3421 ataatacgac cactcaaacg ctctacaaga catgatggat ccactttaat attaataaca
     3481 ccatctaagc ggagtcctag ttcttcaagt gttgcatcta aggcgtgtgc ttgttcaata
     3541 gtacgtggat acccatcaag caaaaaacct ttttctgcga tatcatcctc agctaagcgc
     3601 tcttttacaa tcccgtttgt gacttcatca ggaaccaatt cacctttatc aatataactt
     3661 ttagctaaac gtcccatttc ggtttgatta gccattgcgg cgcggaacat atcccctgtt
     3721 gagatgtgag caataccaaa ttcttcaaca atcttagctg cttgggtccc tttaccagct
     3781 cccggcaaac ccatgattaa aagattcatc ttttcctcct cttttaaaat aaatacatag
     3841 taggaaatta ttttccttgg tttttgtttt caaatggcaa caagaaatcg tttgatgata
     3901 accaagtctt attgtcattt aaaaacaaaa tagaaggttg acaatgtcaa cctgtattct
     3961 attctgctgt attcataaat ccgacatatt ttctctttag aagatatccc tcaagctgtt
     4021 tcataccttc gataccagtt gaaatcaaaa tgagcaaact agttccacct aaagcaatac
     4081 tagaagaaag gttgagagct tgttgtgctg ctattggcgc taaagagata aatgctaaga
     4141 agatagctcc tacggttgct agttttttta gcaatgcgga cataaattgt tctgtctcac
     4201 gtccagggcg aacacttggt atatatgagg aattcttctg aagattttct gctgtcttct
     4261 caggattaac ttgtacaaag gtatagaaga atgagaacaa tataatcaac aaggcgtaaa
     4321 ctatcattcc gactggagtt tgataattaa aaatttcttg caaacggttt aaccacggta
     4381 aatctctacc attttgaaca aaaggaataa tcgtacttgg gatagttgta atcgagctag
     4441 caaagataac gggaataacg ccggctggat ttacttttaa tggaagatat gaacttgtag
     4501 gtgcaccttg cattagcttt gtgtattgga ttggaatttt atattccgct tgttggacat
     4561 atgttgtaaa gaaaacaata gcaagaacag ctatgattaa aatcccaaca atgagataag
     4621 acgagtgtaa atcacttgcc ttaacattta caaaatagtc ttcacgaatt gttgcaattg
     4681 cacttggaat agacgaaata atacccgcaa agataatcat tgatacgcca ttaccaaatc
     4741 ctttatctgt gatttgttct ccaagccagg taacaataac gctacctgtt gtcagtaatg
     4801 ccccgatcag taaatatgtt ttgatgtctg gtgtcttaac aagtgcaacg ttcgacaaag
     4861 tattaaaccc tgcagtgata ccaatggatt gggcaaaagc caaaacaagt gaaatatagc
     4921 gcgtcgcttg atttaattta cgacgtccaa cctcaccttg tttgccccac tcaacaaact
     4981 taggtaaaat atccatctgc aataactgaa caacgataga agcagtaata tatgggctaa
     5041 cccccattga aaatactgag aaatttctca tcgcattacc actaactaag tttaacatat
     5101 ttaagaaagg gagttcactt aattgctcta agctcttggc atttacacca ggtacggtaa
     5161 tatgtgtccc aattcggaat acaagaatga taaatatagt aaagaaaatt ttattccgta
     5221 cagtctttat ctttaacgcg tcttttagta tttttaagaa cataatgagt atcccctcat
     5281 tagatgactt cgattgaacc acctttagca ataatagctg cttcagcaga ttttgagaac
     5341 ttagctgctt ttacagtcaa ttttttagtc aattcaccgt taccaagcac tttaacgcct
     5401 gatttttcag ctcggacaat accagcatct tttaaaatag ccggagtcac ttctgtacca
     5461 tcatcaaaaa cattcagttg atcaagattc acaagtgcat attctttagt attaatattt
     5521 gtaaagccac gttttggaat acgacggaac aatggtgttt gtccaccttc aaaacctaaa
     5581 cgtacaccgc caccgctacg agctttttga cctttttgac cgcgaccaga tgttttacca
     5641 ttacctgatg atgtcccacg accaacacgg ttacgtactt tacgtgagcc ttcagcagct
     5701 tttaattcat gaagtttcat tttttctcct ttttttgtaa aatgctagcg cctctatgag
     5761 ggaaaaggac tccctataga aactcgccta tacattaatg atacttaagt tatccaaaat
     5821 aacttaatac agatacgcac taaccgacta ctgtttgttg ccaaaaagca aactacaaga
     5881 gtcggttttg tgctcgtttt taattatgaa atcagagatt tcacagttat aacttattta
     5941 acatcttcaa cggtaaccaa gtgagaaata gctgtcacca taccacggat agcagcatta
     6001 tcttctttga ctactgaaga gtttaattta ccaagtccaa gagcaacaac agttttacgt
     6061 tgttctggct tacgtccgat aggagactta gtcaaagtaa ttttaatttg agccatgatg
     6121 atcccctttc ttatgctaag tcagaaactg agatgccacg aagtgcggca acttcttctg
     6181 cacgtttaag ttgtttcaat ccttcaacag ttgcacgaac aatgttgatt ggagtgtttg
     6241 aaccaagaga ttttgaagta atatcagcaa cacctgctaa ttcgatgacg gcacgaactg
     6301 caccaccagc agcaactcca gaaccttcta cagctggttt caataatact ttagctccac
     6361 cgaagttagt gtaaacttcg tgagggattg ttgtaccaac cattggtact tcgatcatgt
     6421 ttttcttagc agcttcaact gctttacgga tagcttctgg aacttcttga gctttaccag
     6481 taccaaatcc aacacgacca tttccatcac caacaactac aagagctgca aagcgaagac
     6541 gacgtccacc tttaacaact tttgtaacgc ggttaatcgc aacaacgcgt tcttcaagtt
     6601 caactgcatt atctttaaat gccattattt tgtgtcctcc ctattagaat ttcaatccgt
     6661 tttcacgagc tgcatcagcc aaggctttaa cacgtccgtg atagagatat ccaccgcggt
     6721 caaacaccac ttcagaaata cctttagcca ctgcacgttc agcaacaagt ttgccgacta
     6781 caacggcttg ttctgttttt gttcctttag aaacgtcttt atcaagagtt gatgcacttg
     6841 cgagcgttac acccgctacg tcatcaataa cttgagcgta gatgcctgta ttagaacgga
     6901 atacgttcaa acgtgggcga tcagcagttc cagagagttt accgcggacg cgacggtggc
     6961 gtttttggcg gattttgttt ttatctggtt tcgaaatcac aattttcacc tcttaatttt
     7021 aatcaatttg tctcattgac aagttgtcga ttgaacttat cgagcatgtt aaggtgcaat
     7081 acttcccata cccgactaag aacattattt acctgtttta ccttctttaa ggcgtacgta
     7141 ttcaccaacg taacggatcc ctttgccttt gtaaggctct ggtgaacgca agctacggat
     7201 gtaagcagct gtttgaccaa caacttcttt gttgattcct tcaactgaga ttgaagttgg
     7261 gttagcaaca gtgaaagtaa ttccttctgg agcttcaact tcgtcttggt gagatttacc
     7321 tactgaaagg acaagtttag taccttgaag ttgagcacgg taaccgacac ccttcatttc
     7381 aagatctttt ttgaaacctt cagaaacacc tacaaccatg ttattcaagt tagcacgggt
     7441 tgtaccatgg attgttttca tttcttttga gtcgttagga cgtacaactg tgatttcagt
     7501 cccttcaact ttgatttcaa tatttttgtt gaactcacga gtgagttcgc ctttagggcc
     7561 tttaacagta ataacattgt tgttatttgt taattcaacg cctgcaggca tagtaattac
     7621 tttattacca atacgtgaca tgtttatttt ctcctgttaa attgtcaagc cgcatagcga
     7681 ctagttttca cgggggtttg atagaatgat atacttcaat ttatatttca aaattgaaca
     7741 cgagctgtgg tctgtgtgaa aaagataaat cttccttgag tgtaaacacg ctacgtcaga
     7801 tttcctattt tcactttgtc cacgacgctc ttagtatctt aaattaccaa acgtaagcga
     7861 tcacttctcc accaacattc ttttggcgag cttctttgtc agtcaaaaga ccttctgatg
     7921 ttgaaatgat tgcaattcca agtccgttaa gaactttagg catgtcatca cgttttgcgt
     7981 aaacgcgaag acctggtttt gaaatacgtt ttaagttagt gataacacgt tcaccatttt
     8041 ttccgtattt taagaacaca cgaatgatgc cttgtttatc atcttcgata acttcaacat
     8101 tttttacaaa accttcacgt ttaagaatct cagcaatccc ttttttaatg tttgaagcag
     8161 gtacttctaa cacttcgtgt tttacttgat tagcattacg gatacgtgtt aaaaagtctg
     8221 caattgggtc agtcataacc attttttata ttctcctctt actagcagtt tgatgctatc
     8281 acttactagt taatgttgtc cttaaaggac tttggtatca gcttatgctc acaccgtttt
     8341 aattaagcac aagacttaat ctgaagaaca aattaagttt tgtgtcatat ttttaccaag
     8401 aagctttgac aacacctgga atttgacctt tgtaagccaa ttcacggaag caaacacgac
     8461 aaagtttaaa cttgcggtaa actgaatgtg gacgtccaca tttttcacag cgagtataag
     8521 cttgtgtaga gtgttttgca ggacgtttgt tcttagcaat catagatttt ttagccaatt
     8581 tatttacccc ctattatttt gcaaaaggca ttccaaggcc cttaagcaat tcacgtgatt
     8641 cttcgtcagt atttgcagta gtgacgatta caatatcaag accacgtact ttatcaacat
     8701 catcaaagct gatttctggg aagataagtt gttctttcac accgagagtg tagtttccgc
     8761 gtccatcaaa cgattttgtt ggaacgccat ggaagtcacg tacacgagga agtgaaacgc
     8821 taaccaattt gtctaggaat tcgtacatac gttcgccacg gagggtaact tttgcaccga
     8881 tcgctacacc ttcacgaaga cggaagccag cgattgattt cttagcttta gtaataagtg
     8941 gtttttgacc tgagataagt gctaattcag ctgcagcttt ctctaggttt tttgcgttgg
     9001 atacagcatc accaacaccc atattgagaa caattttctc aacttttggc acagccataa
     9061 ctgatgtgta attgaatttc tctgtcaacg caggaattac ttcgttagta tatttttctt
     9121 ttaaacgatt tgccattatg cttctccttt ccttcgtgat taatcaagca cttcgcctga
     9181 ttttttgctg taacgtactt ttttaccgtc aacaacttta taaccaatac gtccagctac
     9241 accattttta tctaatactt gaacattaga gacatggata ggtgcttctt tttcgacgat
     9301 agctccttga ggattttcag tgttaggttt ttggtgtttt ttaatcattc caacaccttc
     9361 aacaataact ttgttaactt ttggaagtgc tttaagaact acagcttcag tgcctttgtc
     9421 cttgccagca attacgcgaa ctttgtcgcc tttttttaca aacattctgg tttctcctat
     9481 tatttcttac gccccgatgg gcaccctagg aaaacctagg ggactacgtt tgttaatctg
     9541 agattaaagt acttctggtg caagtgatac gatcttcatg tagccaccct cacgcaattc
     9601 gcgtgcaact gggccaaaga tacgagttcc gcgaggagtt ttatcatcac ggatgattac
     9661 agcagcattg tcgtcaaatt tgatgtatga accgtctgga cggcgtgcac cagtttttgt
     9721 acgaacgata acagctttaa ccacatcacc ttttttaact gctccaccag gagtagcttg
     9781 ttttacagaa gcaacgatta cgtcaccgat gttagcgaat ttacgtcctg aaccaccaag
     9841 tactttgata gtcaagatct cacgagcacc gctattatca gcaactttca agcgagtttc
     9901 ttgttgaatc atttcaattt tctcctttta gtttgattag ataataacag ctttttccac
     9961 tacttccaca agacggaaac gttttgtagc tgaaagtggg cgagtttcca tgatacgaac
    10021 gatatcgcct tctttagcaa cgttgttttc gtcatgtgct ttatattttt tagaatagtt
    10081 gatacgttta ccatagactg ggtggttacg tttagtttca actacaactg tgattgtttt
    10141 gtccatcttg tcagacacta cgcgtccgta aagagtttta cgttgattac gttccattta
    10201 gaatttctcc tttcccaatc tattatttca tttctgattg cacagtttta acacgtgcaa
    10261 tttgtttctt aacttcgtca aggcgagcag tcttttcaag ttgacctgct gcagcttgga
    10321 aacgaagatc aaacaattct tttttgagtt cgttttcttt cttagcaagt tcttcttgag
    10381 acaatccacg aagctcttta acaaaatctt taatttcttg aagtttcatg tcttctcctt
    10441 attctgcttc acgttttacg aatttgcact taactggtaa tttgtggcta gcaagacgta
    10501 atgcttcgcg agcaatttct tcagaaacac cagcaatttc aaacatcact ttaccacgtt
    10561 taactggtgc tacccaacct tcaggtgccc ctttaccaga acccatacgt acaccgatag
    10621 cttttgcagt gtatgatttg tgtgggaaga ttttaatcca aactttacca ccacgtttca
    10681 tgtaacgagt catcgcaata cgggcagctt cgatttgacg gtttgtaatc cagtggctag
    10741 ttgtggcttg aagtccgtat tcaccgaatg aaacttcttt accaccttta gcctcaccgc
    10801 gcatttttcc acggaattcg cgacggtgtt taacacgttt aggtactaac atttgttatt
    10861 tgcctccttt agtgttttta cgagctggaa gaacttctcc acggtaaatc caaactttaa
    10921 cgccaagttt accataagtt gtgtcagctt cttcccaagc atagtcgata tcagcgcgaa
    10981 gcgtgtgaag tggaacagtt ccttctgaat aaccttcagc acgagcgata tcagcaccgt
    11041 tcaaacgacc agaaacttga gttttaatcc ctttagctcc tgcacgcatt gtacgttgga
    11101 ttgcttgttt ttgagcacga cggaaagcaa cacgttgctc aagttgacga gcaatgtttt
    11161 caccaacaag gtgagcatct aaatcaggtt gtttgatttc gatgatgttg atgtgtactt
    11221 gttttccagt taatttgtta agttgaccac gaagagcgtc aacgtttgcg ccacctttac
    11281 cgataaccat acctggttta gcagtgtgaa gtgaaacaat aactttgttt actgcacgtt
    11341 caatttcaat agttgaaact gatgcgtcag ctaattcttt attaatgaat ttacggattg
    11401 caagatcttc atgaaggtaa tccgcgtatt ctttttcagc ataccatttc gcatcccagt
    11461 cacggatgat tccgacacgc ataccaattg gatgtacttt ttgacccacg attttacctc
    11521 cttatttttc tgatacaact acagttacgt gagttgtacg tttgttgatt ggtgaagctg
    11581 aacctttcgc acgtggacgg aaacgtttca ttgttggtcc ttcgttagcg aatgtttcag
    11641 atactaccaa gtttgctttt tccaaaccaa agttgttttc tgcgttagca attgctgagt
    11701 taagagtttt ctcaataaca cgagctgctt tgtttggagt gaattttaag attgcgattg
    11761 cgtcagcaac tttcttacca cggataagat caagtactaa acgtgtttta cgaggtgaaa
    11821 cacgcactgt acgagccatt gctttagctg aagtaatttc tgccattgtg tcctcctcct
    11881 attaacgacg tgttttcttg tcgtcagctg cgtgaccttt gtaagtacgc gttggtgcaa
    11941 attcaccaag tttgtgacct accatgtctt cttggatgta aacaggtaca tgtttacgtc
    12001 cgtcataaac tgcgattgta tatccgatga aacttgggaa aatcgttgaa cgacgtgacc
    12061 aagttttgat tacttttttc ttttcgtcgt ttgcttgagc ttcaactttt ttcatcaaat
    12121 gctcatcgac gaaaggtcct tttttaagac tacgtcccat tttgtagttt tctcctttaa
    12181 ataatgtacc acagcggctt gaattcaaga gcaagactcc aaactctacc gagttggcgg
    12241 aataaatagc aaagctattg taacgctatt tcagcaagtt tttagcaact tactaaaatt
    12301 atttttcgtt acgacgacga acgataagtt tgtctgattt agctttcttg ttacgagttt
    12361 taagaccaag cgctggttta ccccatggag ttgatggcgc tttacgtcca actggtgctt
    12421 taccttcacc accaccgtgt gggtgatcgt tagggttcat tacagaacca cgaactgttg
    12481 ggcggatacc tttccaacgg ctacgtcctg ctttaccaat gttaacaagt gattgttgtt
    12541 cgttaccaac agtaccgata gttgcacggc atgtaccaag aatcatacga acttcgcctg
    12601 attgaagacg aacaagaacg tatttacctt cttgaccaag tacttgagca gaagctccag
    12661 ctgcacgaac aagttctcca cctttaccag gtttcaactc aatattgtga acaactgtac
    12721 cgacaggaat gtttgctaat ggaagtgcgt taccaacttt gatatctgca tctggaccag
    12781 aaacaatacg ttgacctact tcaagacctt taggtgcaat gatgtaagct ttcacaccgt
    12841 cagtgtaatg tacaagtgcg atgtttgcag tacggtttgg atcgtattcg attgttttaa
    12901 caactgcttc aacgccatct ttgttacgtt tgaagtcgat caaacggtaa tgacgtttgt
    12961 gtccaccacc ttggtgacga actgtgatgc gaccattgtt gttacgacca gctttgctct
    13021 taagagaaac aagcaatgat ttctcaggcg tgcttgttgt gatttccgcg aaatccaaag
    13081 aagtcatgtt acgacggcca tttgtcgttg gtttataaac tttaataccc acgttatttc
    13141 ctccttagat tattcagctt cagctgcgaa caactcgatt gctttagaat cagctgtaag
    13201 agtgatgata gcttttttag tttttgaagt gaaacctgtg taacgaccaa cgcgttttgc
    13261 ttttggttta acgttaacag tgtttacact tgcaacttta actccgtcaa aagcagcttc
    13321 aacagcttgt ttgatcaaaa gtttgtgtgc acgagtatca acttcgaaag tgtatttgcc
    13381 tgcttcaaga gcaatcattg acttctcagt gataactggt tttttgatta cgtcgtacaa
    13441 attcattatg caagaacctc ctcaattgta gagattgctt ctttagtaac aagaagtttg
    13501 tctgcgttca cgatatcaag aacacttgca gttgctgcag ttgcaacagt tacgtttgga
    13561 aggttacgtg cagaaagtgc tgcaaattca tttccttctt caacaagaac aagtactttt
    13621 gtatcaatgc taagagctga aagcactttt gcaaattcag cagtttttgg tgctgcaaat
    13681 gaaaggcctt ctacagctac aaatttatct tcagcaactt ttgctgagta aactgatttc
    13741 aacgcaaggc gacgaacttt ttgtggaagt ttgtatccgt atgaacgggg agttggtcca
    13801 aagacaacac caccaccacg ccattgtggt gagcggatag aaccttgacg agcgcgtcca
    13861 gttccttttt gacgccatgg tttacgtcca ccacctgata ctgctgaacg gtttttaacc
    13921 gcatgagtac cttggcgaag gctagcgcgt tggctaatta caacatcaaa aacaactgat
    13981 tcgtttggtt cgataccgaa gatagcgtcg tttaattcaa ctgagctaac ttctttacca
    14041 gtttggtcaa atagttttac gtttgccatt ttaactgttt tctcctttct tattatttag
    14101 cagctttaac tgctgacttg atagtgataa gagatttctt agcacctggt acgttacctt
    14161 taacaaggat aacgttcttt tctgggataa cttgtacaat ttcaaggttt tgaactgtta
    14221 cacggttacc acccatacgt cctgccaagc gtttgttttt gaaaacgcgg ttaggcgcaa
    14281 caggtcccat agaacctggg cgacgatggt aacgagaacc gtgagccata ggaccacgtg
    14341 attgaccatg gcgtttgata acaccttgga aacctttacc ttttgatgtc cctgtgacgt
    14401 caacaacatc accagcttca aattgttcta cagataattc tgcaccaact tctaagcctt
    14461 caatgttttt gaattcacga atgaagcgct taggagctgt gtttgctttt gcaacatggc
    14521 ctttggcagg tttgttactc aagacttcac gtttgtcatc aaaaccaacc tgaactgctt
    14581 cataaccgtc tgtttcaaca gttttaactt gaagcacaac gtttggagtt gcttcaatga
    14641 cagtaacagg gatgaattcg cctgattcag tgaagatttg agtcattccc acttttttcc
    14701 ctaagattcc ttttgtcatg agaaaatatt tccttttctt aataatattt caaaaagttt
    14761 tttacgagcg ttttttgtgc tcatagcaag atacaaagag cgtcaaactc aaaataaaaa
    14821 tagaaaatct aacacaaaag cttctgtttc gaggaagatt tatctttttc atgtagagtt
    14881 tagctcgtgt tcaagttcca tcaaacacta actgtacttg caaaatctca ccgattaaag
    14941 tttgatttct acgttgacac cacttggaag atcaagtttc atcaaagcat caacagtttt
    15001 ttgtgttgga ttgatgatgt ctaccaaacg tttgtgtgta cgcatttcaa attgttcgcg
    15061 agaatctttg tatttgtgag tcgcacgaat aattgtgtaa agactgcgtt cagttggaag
    15121 tggaactggt ccagcaactg tagcacctgt acgtgttgca gtttcaacga ttttttctgc
    15181 cgctgtatca agtgtacggt gttcgtacgc tttcaaacgg atacggattt ttttgtttgc
    15241 catcttttct cctctcgtct atttacgata ataggctagc tcctcaagaa aaccgacacc
    15301 tgttgcgtgg caatgcaacc gagcgtgtcg caacctcttg tatcattgct aaagctgtat
    15361 ttttacagca ccacaatata ataacagaat catttagtca ttgcaagctt tttaggcatt
    15421 tttttcaaaa aaaaactacc ttcctaatga aaggtaattt cttggtactt tttaaagtgt
    15481 ttagagagaa taaaccaccg actggtatta tctaacactg tcgccatcca gacccctatg
    15541 agtccatatt gccaaaccac accaatcaca taaccaaggc caatgcgaat cacccacatt
    15601 cctatggttg ttgcatagaa aggaagcttt gcttttccca aaccctgcca gacggctgta
    15661 taaactaaag tgcctgccgt tgcgggagct cctagtaaag aaaaaagtaa aacaatcatg
    15721 gcagaccttt gagcatcagt attctgtgta aatagcggca agagactagg acctagcaga
    15781 taggtcaagg ctcccataac cagcatcatg agggtggata aaataaaagc ttctctaata
    15841 atatacctta tctcagtcac cttaccgccc cccagttgcc tagccactag aataattgtt
    15901 gccgttgcca tggctaagcc aggcatgtaa ttaaactgag tcaaggtttc tccaatagca
    15961 ttacctgcca aggccgttgt cccaaagcga acaacaatgc caataattag gacatctcct
    16021 gctctcatca tcaaacgttc accagcagca ggtagtgata aatcaaaaat aattttgtcc
    16081 aaaggtctca tcaatcgttt cgcaacctgt ttaataggaa taaactgaca aagtaaaaac
    16141 acacccacta aacgagataa aaccgtcgcc caagcaacac caagaagacc aaatccccat
    16201 acatagatag acaaggcaga aaaaatggca ttgagtacgt ttattaataa gctcacttgc
    16261 ataggaatct taggataccc ttgagctcga acaatagctc ctaagctagt taaaagtcct
    16321 agactaacaa tcataccgcc gacaatggat agatattgcc ctccaaccaa tgttacagaa
    16381 gcttcagctc ctagccattc taacacctga tgatgtccag caactgataa caaccctaag
    16441 ccgaccgaaa ggaggagtgt tacttgcaag acacctgcca tatagtttaa ttgtttgttc
    16501 tgattatttt ctccaatact tcgagcaatc agactagata cagcagctcc cagcgcgata
    16561 aagagggact gataaatact gataatgttg ttagctatgg agacaccaga gactgctaca
    16621 agtccaattt gagccaccag gtaattatca accattccca ttaacatctg taaaatattt
    16681 tctatcatag aaggcagagc taaagagaaa atttttcttc tattattata tatcatttat
    16741 gtcccctaat aaaaaattct tgatcaaacg attttttcaa acacagtcac tggactcatt
    16801 cagttatttt ggtccattaa tgaattcata aagtctatct taatagcaga gtcccaagat
    16861 acacgacact tagtgttaca gtaacaattg accacatgcc aatccttaac atttacctaa
    16921 cgtttataac taagaagatg aagaacaaaa ggactccaaa cgaatttttt ctattatata
    16981 atatattctt catttagcaa ctttcaatgt atcaaaatag cactctcgag tcaaaaatgt
    17041 tttctgatac tcgagagtgc tattttattt ggaatcaagt ttaagcaact taggcttatc
    17101 tcatatttaa tacatttgta tgtttagctg atgaaatcta agaccttgcg cccttggatt
    17161 aaaccacgtt ccatttcatc aaagacttct ggagcagtgt caactggaac tttttcgaca
    17221 accggagcaa ccagaccttc tgcaccaaag gcaaaggctt cttccaagtc tttacgtgtc
    17281 cctacaagtg atccgaccac tttgatacca tctaaaacag tttttacgat agacagttcc
    17341 atgtattctg atggaagacc tacggctaca actgttccac cggcacggac actatcaatt
    17401 gcttggttaa aagcaacttt agaaacagcc gttaccacaa caccatgtgc tccaccggtt
    17461 ttttcttgaa tatagcctgg aacatcttca atttctttgc cgttaactaa aatatctgcg
    17521 ccaacttctt tagctaactc aagtttatca ttattaatgt ccactgcgac aacatgtgca
    17581 ttaaagactt ttttagcgta ttgaacagcc agatttccaa ggccaccagc cccaaagata
    17641 acaatccatt ggcctggagc agcacctgcc tctttaatag ctttataagt tgttacacca
    17701 gcacaagtaa tggaagaagc ttgagccggg tctaaacctt ctggaacttt aactgcataa
    17761 tcagctgtta ctactgcata ttcactcatt cctccatcaa cactgtatcc agcattttta
    17821 acactacgac aaagtgtctc acgtcctgta gtacagtatt cgcaatgacc acatccttca
    17881 aagaaccatg caatagagac acgatcacca acctttaagg atgttacgcc ttctccaatt
    17941 tcttctacta taccaatacc ttcgtgcccc aaaatacgtc ctggtacctg accaaaatcg
    18001 ccatgtgcaa cgtgtaaatc agtatgacaa acgccacagt attctacttt aacaagcgct
    18061 tcaccatgac caatatttgg aagatcatgc tcaacaactt cgacacctgt actagcttga
    18121 ttaacaacta cggctttcat atcagtcctc catctaataa tgactttttt gtaatcggtt
    18181 acaaataaat tgtacatctt ttcacaatga aagtcaatag aaacctgaaa agtttctgaa
    18241 acttaactgg ttaatcttat agctgtttgc ctatctgcaa aatacttaac tacattttct
    18301 gacactggat ataaatgata tatttagaaa ataaaatgat ttcttatgac tttaatcata
    18361 agaaatcatt tcctggtatc aacattaaaa atcacttacg tcgtcctgga cgttcagcat
    18421 aaccatagta agcatcttca ataatttctt gcatgtgatc aaccattgga agacgtgggt
    18481 tagcaggtga acattggtct tcataagcaa ggtaagccaa ttcacgagaa tgttctttcc
    18541 attcgttttc gtcgatacct tgagctttga agttcatttg aataccaact cggcaaccca
    18601 gatcataaac agctttcgca taagattcca ctgcttcttc tggtgtagaa gctggaagcc
    18661 ctaacaattt cgcaatgtct tggtattttt catccgcacg gtagtaatta tacttaggcc
    18721 atgtcgccgt tttagctgga cgagtcccat tatagcggat aacatacggc aaaagaattg
    18781 catttgtacg tccgtgtacg gtatggaatt gtgcgccaat tttatgcgcc attgagtgag
    18841 aaatacctaa gaatgcattg gcgaaagcca tacctgccat agttgacgca ttatgcatct
    18901 tttcacgtgc ttcaaaatcg gctgttttaa ctgatttttc aagattatca aaaacaattt
    18961 taatggcttg aagagctaaa ccatccgtaa agtcattggc catttgtgac acgtaagctt
    19021 cggttgcgtg tgtcaaaacg tccataccag tatcagcagc aataaagcct ggaacagtca
    19081 ataccaaagc tgggtcaaca atggctacag ttggtgttaa tgagtaatca gcgattggat
    19141 atttacgatt atttgctttg tcagagataa cggcaaatgg tgtcacctct gaacctgttc
    19201 cagaagttgt tgggatagca acaaattttg ttttcttacc aagttctggg aatttgaacg
    19261 cacgtttgcg gatatccatg aatttttgaa cgaggtcatg gaaatcaact tctggttgct
    19321 cgtagaagag ccacataact ttggctgcat ccatagggga tcccccgcca agggcaataa
    19381 tggtgtctgg tttgaaggta cgcattaatt cagtaccttt cataacagtt gtaatgtctg
    19441 gatctggttc tacttcagca aagatctgat aaacaacttt attgcgacga agatcaagtt
    19501 gttcaatgat acgatccaag aaaccaagct caaccatagc gtgatctgta acaatcatga
    19561 cacgttcaac gtcacgacat ttttgcaaat attgaattga atcacgttcg aagtatgttt
    19621 ttgaaggaac tttaaaccat tgcatattat ttctacgtct tcctactttt ttgatgttca
    19681 agagatttat agcactaacg ttatcaccaa ctgcgttacg tccatacgaa ccacaaccaa
    19741 gagtcaatga tggtaagaaa gcattgtaaa catccccgat accaccaaat gtagaaggtg
    19801 aattccagat gacgcggata gcacggattc tagttccaaa ttcttttgcc aattctgcat
    19861 ctgctgtatg aatggcagct gagtgaccga gtccattaaa ttcaaccatt tgacgagctt
    19921 tttcaacacc gtcttcacgt gattcagatt ttaaaacagc aatgactgga gataattttt
    19981 cacgtgttaa aggctcattt tctgaaactt ctttacattc tgcagcaaga atattggttc
    20041 cttctggaac agtaaatcca gcttgttctg caatccaagt agctggtttt cctacaatat
    20101 ttggatttaa ttttgcacca gcacagtttt tgctatttgc tttggcacca aaacagaatt
    20161 cttcaagaag ggctttttct tttttattta caaaataagt atggtaagat ttgaattctg
    20221 ctacaaaatc gtcatagatt tctttatcga tgataacagc ttgttcagag gcacagacca
    20281 taccattatc aaatgattta gacatcacaa tatcatgagc ggcttgacgg atattagctg
    20341 atttttcaac ataagctgga acgttaccag cacctacccc aagggcaggt ttcccacatg
    20401 agtatgccgc tttaaccatc gcatttccac ctgtcgcaag gatagtggca atgccgtcat
    20461 gattcatcaa agcatttgtt gcttcaagtg atggtgtttc aacccactgc acacaatttt
    20521 caggagcacc agcggcaata gcagcatcac gaacaatacg agcggcatgt gctgatgatt
    20581 cttgtgctga tgggtgaaag gcaaaaataa ttggattacg tgtttttaac gaaatcaaag
    20641 atttaaagat agctgttgaa gttgggttag ttgttggtgt aatcccacag ataacaccca
    20701 ccggctcagc aattaatgtc aaacctgtca catcatcttc ttcaataatt cctactgttt
    20761 tttgatggcg catgttatta accacatgct cacaagcaaa tagatttttt gttgctttat
    20821 cttcaaagac accgcgacct gtttcttcat aagcatgttt agcaagttca ccgtgagcat
    20881 ccaaagcagc aacagaagct ttggctacga tataatcaac ttgttcttgg tcaagcttac
    20941 gcatctcttc aagagcagct aaaccttttt gaacaagagc atcaattgtc acagaaacag
    21001 aggttgtttc aacagtatta tgtccttcag tcatttgaga ctcctttata ttgttaaaag
    21061 tttcacaatt ttgtttacaa aattattata gcactttttg aaattttgta aatcatttca
    21121 caataaattt gtgaagtttt tttcaa
//
LOCUS       DAXWIQ010000031        20829 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000031 DAXWIQ010000000
VERSION     DAXWIQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20829
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.031"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(<1..308)
                     /gene="cadX"
                     /locus_tag="ACGEUI_001197"
     CDS             complement(<1..308)
                     /gene="cadX"
                     /locus_tag="ACGEUI_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HFF8166037.1"
                     /translation="MKKDSICQVGVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQNILDTRREGKIIYYFIKDE
                     "
     gene            complement(320..934)
                     /locus_tag="ACGEUI_001198"
     CDS             complement(320..934)
                     /locus_tag="ACGEUI_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFF8166038.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKEGLSK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            963..1187
                     /locus_tag="ACGEUI_001199"
                     /pseudo
     CDS             963..1187
                     /locus_tag="ACGEUI_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002892741.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FanG protein"
     gene            complement(1227..1376)
                     /gene="rpmG"
                     /locus_tag="ACGEUI_001200"
     CDS             complement(1227..1376)
                     /gene="rpmG"
                     /locus_tag="ACGEUI_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8166039.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVT
                     FTEVK"
     gene            complement(1392..1574)
                     /gene="rpmF"
                     /locus_tag="ACGEUI_001201"
     CDS             complement(1392..1574)
                     /gene="rpmF"
                     /locus_tag="ACGEUI_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFF8166040.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            1794..3074
                     /gene="hisS"
                     /locus_tag="ACGEUI_001202"
     CDS             1794..3074
                     /gene="hisS"
                     /locus_tag="ACGEUI_001202"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFF8166041.1"
                     /translation="MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKD
                     VTLHLNSLGSPESRAAYRQALIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLA
                     VEKAPSILDYLDEESQAHFEAVKDMLEALDIPYVIDTNMVRGLDYYSHTIFEFITSVE
                     GSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMIIEKQGITLPIETEMDIYLA
                     VLGDGANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEA
                     GKAVIKNNRSRQEVEVSFEDMMTNFANISEQLLS"
     gene            3412..5160
                     /gene="aspS"
                     /locus_tag="ACGEUI_001203"
     CDS             3412..5160
                     /gene="aspS"
                     /locus_tag="ACGEUI_001203"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFF8166042.1"
                     /translation="MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREG
                     VMQLVINPEEVSSNVMATAERLRSEYVIEVEGFVEARQQANDKLATGMVELKVSALTI
                     LNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
                     IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSY
                     DVAMNSYGSDKPDTRFEMLLQDLTVTVKGNDFKVFSEAPAVKAIVVKGNADRYSRKDI
                     DKLTEFAKQFGAKGLAWVKVTDGQLAGPVAKFLTAIETELSSQLKLAENDLVLFVADT
                     LEVANNTLGALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     PESAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKEMQERMFKALGFTADEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SENQLEELSLQIESHD"
     gene            5153..6094
                     /locus_tag="ACGEUI_001204"
     CDS             5153..6094
                     /locus_tag="ACGEUI_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8166043.1"
                     /translation="MIKKTTYKKKVKYVISRGAKKVGLLHALRSISREKYAEKISASL
                     LYGILSSIAVNFFFQPGHVYSSGATGLAQVFSALSHRLLGYDFPIAFAFYLINIPLLI
                     LAWYKIGHQFTIFTFITVSMSSFFIQIMPQVTLTTDPLINAIFGGLVMGMGIGTGLKS
                     RISSGGTDIVSLTLRKRTGKDVGSLSLMVNGAILAFAGILFGWQYALYSMVSIFVSSR
                     VTDAIFTKQKKMQATIVTSHPERVIHMIHKRLHRGVTSINDAEGTYKHEQKAVLITIL
                     TCEEYPEFKWLMLKTDPQAFVSVAENVRIIGRFVEDD"
     gene            6114..6992
                     /locus_tag="ACGEUI_001205"
     CDS             6114..6992
                     /locus_tag="ACGEUI_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8166044.1"
                     /translation="MSIDQRLQLKKFCFIILGAAIYAFAFVYFYMANRIAANGLAGLT
                     LVGKALFEIDPSLIGYLINLPLVLLGARCFGKRAMVYTVTGILSLYFFVWLFQRFPLV
                     VDLDHDNLVVSLMAGIIGGLGGGLVFRNGGTIGGADIIAKLLEDKLGLQLNQALLGID
                     LFVMIVSLTYISLPQMMYALIASFMYGQIVRLVQQGGYSARGIFIVSEQAEKIARFIM
                     DELGRGVTYLTGEGAYSGRRKKVIYVALGRRDIRELKAFLTQVDPNAFITYFDVNEVN
                     SPEFLTIKSKYHKKRK"
     gene            7035..7907
                     /locus_tag="ACGEUI_001206"
     CDS             7035..7907
                     /locus_tag="ACGEUI_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8166045.1"
                     /translation="MAHHDKLTKLLKLFLIALGVAIYTFGFVNFNMANALAEGGVAGI
                     TLILHAHFGINPAYSSLLFNLPLFILGAKIFGKRSLALTIYGTVLMSAFIWMWQKVPI
                     ELGLENDMMLVAVVAGLFSGIGSGIVFRYGATTGGTDIIGRIAEEKFGAKLGQTLLLV
                     DALVLTASLTYVDLKHMLYTLVASFVFSQMISVVQNGGYTIRGMIIITKHSEAAAQAI
                     LTEINRGVTYLKGQGAYSGNDYNIMYVTLNPTEVREVKRILAGLDPDAFISIIDVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(7934..8242)
                     /locus_tag="ACGEUI_001207"
     CDS             complement(7934..8242)
                     /locus_tag="ACGEUI_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFF8166046.1"
                     /translation="MPSEKEILDALSKVYSEQVIQADDYFRQAIFELASQLEKEGMSS
                     LLATKIDSLINQYILTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(8330..10021)
                     /gene="argS"
                     /locus_tag="ACGEUI_001208"
     CDS             complement(8330..10021)
                     /gene="argS"
                     /locus_tag="ACGEUI_001208"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFF8166047.1"
                     /translation="MDTKTLIASEIAKVVPELEQDAIFNLLETPKNSDMGDLAFPAFS
                     LAKVLRKAPQMIASELAEQIDESQFEKVVAVGPYINFFLDKAKISSQVLEQVITAGSD
                     YAQQDEGQGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLAHIFAKMGYKPVKINHLGD
                     WGKQFGMLIVAYKKWGDEAAVQAHPIDELLKLYVRINAEAETDPTVDEEAREWFRKLE
                     DGDKEATELWQWFRDESLLEFNRLYDQLHVTFDSYNGEAFYNDKMDEVLDLLEAKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSVYVVGNE
                     QAAHFKQLKAVLKEMGYDWSDDMTHVAFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKEAVAHAVGVGAIKFYDLKTDRMNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKADFTPSATTTYSLADAESWEIIKLIQDFPRIIKRTSDNFEPSIMA
                     KFAINLAQSFNKYYAHTRILDDNSERDNRLALCYATATVLKEALRLLGVDAPNEM"
     gene            10312..10749
                     /gene="argR"
                     /locus_tag="ACGEUI_001209"
     CDS             10312..10749
                     /gene="argR"
                     /locus_tag="ACGEUI_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8166048.1"
                     /translation="MNKMERQQQIKRIIQAEHIGTQEDIKNHLQKEGIVVTQATLSRD
                     LREIGLLKLRDEQGKLYYSLSEPVATPFSPEVRFYVLKVDRAGFMLVLHTNLGEADVL
                     ANLIDNDAIEDILGTIAGADTLLVICRDEEIAKRFEKDLAAGL"
     gene            10767..11098
                     /locus_tag="ACGEUI_001210"
                     /pseudo
     CDS             10767..11098
                     /locus_tag="ACGEUI_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623648.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
     gene            11085..13640
                     /gene="mutS"
                     /locus_tag="ACGEUI_001211"
     CDS             11085..13640
                     /gene="mutS"
                     /locus_tag="ACGEUI_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFF8166049.1"
                     /translation="MAKTNISPGMQQYLDIKKDYPDAFLLFRMGDFYELFYEDAVKAA
                     QLLEIGLTSRNKNAENPIPMAGVPHHSAQQYIDVLIELGYKVAVAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSAKPDSANNFLVAVDFDGCRYGLAYMDVSTGEFCVTDLADFTS
                     VRSEIQNLKAKEVLLGFDLSEEEQTILVKQMNLLLSYEETVYEDKSLIDGQLTTVELT
                     AAGKLLQYVHKTQMRELSHLQALVHYEIKDYLQMSYATKSSLDLVENARTNKKHGSLY
                     WLLDETKTAMGMRLLRSWIDRPLVSKEAILERQEIIQVFLNAFIERTDLSNSLKGVYD
                     IERLSSRVSFGKANPKDLLQLGHTLAQVPYIKAILESFDSPCVDKLVNDIDSLPELEY
                     LIRTAIDPDAPATISEGSIIRNGFDERLDHYRKVMREGTGWIADIEAKERQASGINNL
                     KIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGQMLEAREESS
                     SLEYDIFMCIRAQVETYINRLQKLAKILATVDVLQSLAVVAETNHYIRPQFNDNHVIT
                     IQEGRHAVVEKVMGVQEYIPNSISFDQQTSIQLITGPNMSGKSTYMRQLALTVIMAQM
                     GSFVAADHVDLPLFDAIFTRIGAADDLISGQSTFMVEMMEANQAIKRASDNSLILFDE
                     LGRGTATYDGMALAQAIIEYIHDRVGAKTIFATHYHELTDLSTNLTSLVNVHVATLEK
                     DGDVTFLHKIAEGPADKSYGIHVAKIAGLPKSLLKRADEVLTRLETQSRSTEIISVPS
                     QVESSSAVRQGQLSLFGDEEKAHEIRQALEVIDVMNMTPLQAMTTLYELKKLL"
     gene            13769..15751
                     /gene="mutL"
                     /locus_tag="ACGEUI_001212"
     CDS             13769..15751
                     /gene="mutL"
                     /locus_tag="ACGEUI_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFF8166050.1"
                     /translation="MTNIIELPEVLANQIAAGEVVERPASVVKELVENAIDAKSSQIT
                     VEIEESGLKMIQVTDNGEGMSHEDLPLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     VASISKITIKTATKEVTHGSLLIATGGEIETLEAISTPTGTKIKVENLFYNTPARLKY
                     MKSLQAELAHIVDVVNRLSLAHPEVAFTLISDGRQLTQTSGTGDLRQAIAGIYGLNTT
                     KKMLAISNADLDFEVSGYVSLPELTRANRNYMTILVNGRYIKNFLLNRAILDGYGSKL
                     MVGRFPIVVIDIQIDPYLADVNVHPTKQEVRISKERELMALISTAISESLKEQDLIPD
                     ALENLAKSSTRHFSKPEQTQLPLQSRGLYYDPQKNDFFVKESAVSEKIPETDFYSGAV
                     DNSVKVEKVELLPHSEEVIGPSSVKHASRPQNTFTETDHPNLDLKNRQKLSQMLTRLE
                     NEGQSVFPELDYFGQMHGTYLFAQGKDGLFIIDQHAAQERVKYEYYRDKIGEVDSSLQ
                     QLLVPYLFEFSGSDFINLQEKMALLNEVGIFLEVYGHNTFILREHPIWMKEEEIASGV
                     YEMCDMLLLTNEVSIKTYRAELAIMMSCKRSIKANHSLDDYSARNLLLQLAQCQNPYN
                     CPHGRPVLINFSKADMEKMFRRIQENHTSLRELGKY"
     gene            15762..16982
                     /locus_tag="ACGEUI_001213"
     CDS             15762..16982
                     /locus_tag="ACGEUI_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFF8166051.1"
                     /translation="MQEFLNLPKQIQLRQLVRFVTITLGSSIFPFMAMYYTTYFGTFW
                     TGLLMMITSLMGFVGTLYGGHLSDALGRKKVIMIGSVGTTLGWFLTILANLPNAAIPW
                     LTFAGILLVEIASSFYGPAYEAMLIDLTDESNRRFVYTINYWFINIAVMFGAGLSGLF
                     YDHHFLALLVALLLVNVLCFGVAYYCFDETRPETHAFDHGKGLLASFQNYRQVFHDRA
                     FVLFTLGAIFSGSIWMQMDNYVPVHLKLYFQPTAVLGFQVTSSKMLSLMVLTNTLLIV
                     LFMTVVNKLTEKWKLLPQLVVGSLLFTLGMLLSFTFTQFYAIWLSVVLLTFGEMINVS
                     ASQVLRADMMDHSQIGSYTGFVSMAQPLGAILASLLVSVSHFTGPLGVQCLFAVIALL
                     GIYFTVVSAKMKKV"
     gene            16984..17580
                     /gene="ruvA"
                     /locus_tag="ACGEUI_001214"
     CDS             16984..17580
                     /gene="ruvA"
                     /locus_tag="ACGEUI_001214"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFF8166052.1"
                     /translation="MYDYIKGQLTKITAKYIVVEANGLGYMINVANPYSFTDSVNQLV
                     TIYLHQVIREDAHLLFGFHTEDEKDVFLKLISVSGIGPTTALAIVAVDDNEGLVNAID
                     NSDIKYLMKFPKIGKKTAQQMVLDLAGKFVEAPQETGHTKARSNKAGNTQLDEAIEAL
                     LALGYKAKELKKIRAFFEGTSETAEQYIKSALKLLMKG"
     gene            17590..18150
                     /locus_tag="ACGEUI_001215"
     CDS             17590..18150
                     /locus_tag="ACGEUI_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFF8166053.1"
                     /translation="MKRCSWVPKDNQLYCDYHDLEWGQPLDDDRDFFELLCLESYQSG
                     LSWLTVLKKRQAFRTVFHHYDIASVATFTSEEMADALENPSIIRHKLKLAATVNNAIA
                     VQKIQKEFGSFSTYLWNFVGGKPINNLVNQENLVPAQTELSIRLAKDLKKRGFKFLGP
                     TTVYSFMQASGLVNDHEEACVFNRIF"
     gene            18219..19490
                     /locus_tag="ACGEUI_001216"
     CDS             18219..19490
                     /locus_tag="ACGEUI_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFF8166054.1"
                     /translation="MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGD
                     NEERLLSVITTASQRSNLVILCGGLGPTKDDLTKQTLAKYLRKDLVYDEQACQKLDDF
                     FAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKPM
                     VNEELVPLLSKQYSTLYSKVLRFFGIGESQLVTVLSDFIENQTDPTIAPYAKTGEVTL
                     RLSTKTENQALADKKLGQLEAQLLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKY
                     DKTITAAESLTAGLFQSTLASFPGASQVFNGGFVTYSMEEKAKMLGLPLEELKSHGVV
                     SAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLI
                     SGRSRLDVRYIATLHAFNMVRKTLLKLENLL"
     gene            19579..20715
                     /gene="recA"
                     /locus_tag="ACGEUI_001217"
     CDS             19579..20715
                     /gene="recA"
                     /locus_tag="ACGEUI_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFF8166055.1"
                     /translation="MAKKLKKNEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGTTQIKGTGDQKDSSIGKETKIKVVKNKVAPPFKVAE
                     VEIMYGEGISRTGELVKIASDLDIIQKAGAWFSYNGEKIGQGSENAKRYLADHPELFD
                     EIDLKVRVKFGLLEESEEESAMAVASEETDDLALDLDNGIEIED"
BASE COUNT         6195 a         3593 c         4552 g         6489 t
ORIGIN      
        1 tcctcatctt ttataaaata atatataatt ttcccctctc ttctagtgtc caagatgttt
       61 tgattggcta gtttacgaag atggtgggag gcagatgcca tactgagatt taataaacag
      121 gctatatcgc agacacagag ttcttcgacg gcaaggagat aaaagatgat atttatctgt
      181 ttattatcgg taaattttga taaaatacga agtgattttt ggactttttc cttttcaagg
      241 tagttcgttg cggttgtaac attttgttga tttataacac ccacttgaca gatactatct
      301 tttttcataa ttttttctcc tagcctaaca cagcccatag catgtcaaag ctgttgtttt
      361 caatcaggat atacatcccc aatcctaaat aaacaacggc aataaaccat ctgctatatt
      421 tttccaaagt ttctccaaca gaagggactt gtgccaattt ttgggcagaa aaaaccaaga
      481 gataaatcat gactagaaag gtaagtaaag ccactatcaa attcgctaaa tttaaggtag
      541 taaaatatgg gacaaagaca ccaatattgt cagcaccaca acttgcaaaa gtaatcatag
      601 cgactagaaa aatcaggttt ttattatctt tgctcaaacc ttctttggca atagcttctc
      661 catcagaatc tcctaaaagc aaaactttga ggcctaggaa aattggaatc aaaccgagca
      721 aacctaaaat ctctttacta ggaatataat ctaagacaaa tgcaaaaagc aaacttagca
      781 atattagact aacagagcct agaaattgtc ctaaatagat gttaatgatg tcttttctgc
      841 tttttctttt ggcaaaaaat aacattagga taataagtaa gtctacggct gtcccagaat
      901 acaggattat tgaagtaaca acattttgaa tcataaaaca cctcattcaa atatattttt
      961 gaatgaattt taacattaaa ctttgtagat gtcaatttca gctacaccaa aatatagata
     1021 agaagttagt gtaccaaata taaaaaagcc ctgccatcca aatgataaca gggcttaact
     1081 tcaatatcca aatgatatat tcatctaaaa aaggtagagt aaaaggtaga gttcttattg
     1141 atttatatta caatgtaatg aacttcaaaa attcaaaaat cgcttaaaat caatgttttg
     1201 acgtctaatg acgtgtagtg aaccccttac ttaacctctg taaatgtaac gtgtttacgt
     1261 aattttggtg agtatttttt caattgaaga cggtctggag tgttacgttt gttttttgaa
     1321 gtaaggtaca agcgttcacc agattcttta tgttcaagtg taatatttac gcgcatggta
     1381 tctcccttct attatttagc ttcgttagct ttagcgattt tacgtccttt gtagtatcct
     1441 ttaagggata cacggtgaga acgtgagtaa tctccagtag tttcgtcgaa ttgtacagat
     1501 ggagctgtca atttgtagtg tgtacgacgt ttgtttttct tcgcttttga cgtgtgacgt
     1561 gcaggtactg ccattttctt gttcttcctt tcgatgtgta agttttgatt ttcatcaact
     1621 tcactatgat aacatatctt ttttgtaaag taaagttact tgacaaaact ttttattttt
     1681 acaatttcat ggtgaaaata ctatcaaact aaagcaaatg atatatgctt gtttaaaata
     1741 taagaaaaaa tggtagaatg tttaaagaga aaactaatca aagaggtaaa cttatgaaac
     1801 ttcaaaaacc aaaaggaacc caggatattc tgcctgggga tgctgcgaaa tggcagtatg
     1861 tggaatctgt tgcgcgtgat acgtttagcc aatataatta tggagaaatt cgtactccta
     1921 tgtttgaaca ctacgaggta atctcgcggt ctgtaggtga tacgactgat attgtgacga
     1981 aggaaatgta tgatttctat gataaaggag atcgtcacat taccttgcga ccagagggta
     2041 cagctccagt ggtgcgttct tatgttgaaa ataaattgtt tgcgccagag gtccaaaaac
     2101 ccgtcaaact ttattatatc ggttccatgt tccgctacga acgtcctcaa gctggtcgtt
     2161 tacgtgaatt tcaccagatt ggggtagaat gtttcggcgc tgctaatcca gcaacagatg
     2221 ttgaaaccat tgcgatggcc tatcacctct ttgaaaaatt gggcattaag gatgtcaccc
     2281 ttcatttaaa tagtttgggg agtcctgaga gtcgtgcagc ttatcgtcaa gccttgattg
     2341 actacttaac accaatgcgt gatcaattat ctaaagatag tcaacgtcgc ttggatgaaa
     2401 acccactgcg cgtgcttgat tctaaagaaa aagaagataa actagccgtt gaaaaagcgc
     2461 cttctatttt ggattactta gatgaggaaa gtcaggcgca ctttgaggcg gtcaaagata
     2521 tgcttgaagc acttgacatt ccttatgtga ttgacaccaa tatggtgcgt ggccttgatt
     2581 actatagcca cactattttt gaattcatca cgtctgtgga aggttctgac ttaactatct
     2641 gtgcgggtgg acgttatgat agtttggtag gttattttgg tgggcctgag actccaggat
     2701 ttggctttgg tcttggttta gaacgtctct tgatgattat tgaaaagcag ggcattacct
     2761 tgccaattga gacagagatg gatatttacc ttgctgtgtt aggtgatggt gctaatagca
     2821 aagctttaga attggttcaa gccattcgcc gtcaagggtt tacagctgaa cgagattacc
     2881 ttggccgtaa gattaaggct cagtttaaat cagccgatac ctttaaagca aaattggtaa
     2941 tgactttagg agaaagtgaa gttgaagcag gcaaagctgt gattaagaat aatcgtagtc
     3001 gtcaagaagt ggaagtcagt tttgaagata tgatgacaaa ctttgcgaac atctcagagc
     3061 aattattatc ttaatgattt tattgtcgtc ataagcttgt gttgatatga ttgtttagaa
     3121 gataatcact aaaacaggtg taacttctct ttttagaagg gagttgcacc tgtttgtttt
     3181 ctgtaagggg attgattagc tttcttagta ttctgtgagg acacttgata tgtcaaaata
     3241 ctgacggctc agtatttttg ctttatcttt agaaaaactc tttttaaatg ggtggggttt
     3301 ttaagggctt tttgctataa tagtagggaa tgaattaatt gatttatata gtgaaaggaa
     3361 ttggaaacga gcaggtttca caggattctg tttgttttag ggaatttatt tatgaaacgt
     3421 agcatgtatg cagggcgtgt tcgcgaagaa cacattggaa cgacaatcac attgaaagga
     3481 tgggtcagtc gtcgtcgtga cttgggtggt ctcatcttta ttgatttgcg tgaccgtgag
     3541 ggtgtgatgc aattagtcat taaccctgag gaagtttcca gtaatgtgat ggcaactgct
     3601 gaacgcttac gtagtgaata tgtgattgaa gtggaaggtt ttgttgaagc tcgacaacaa
     3661 gccaatgata agttggcgac aggaatggtt gagttaaaag tatctgcttt gaccatttta
     3721 aacacagcta aaacgacgcc ttttgaaatt aaagatgacg ttgaagtaag tgatgacacg
     3781 cgtttacgtt accgttactt agatttacga cgtccagaaa tgttagaaaa cttcaagcta
     3841 cgtgcaaaag tgactcattc tatccgtaat taccttgatg accttgagtt tatcgatgtc
     3901 gagacaccta tgttgacaaa atcaactcca gaaggtgcgc gtgactatct ggtaccaagt
     3961 cgtgttagcc aagggcattt ctatgctttg ccacaaagtc cgcaaataac caaacagtta
     4021 ttgatgaatg ctggctttga tcgctattat caaattgtga aatgtttccg cgatgaagat
     4081 ctacgtggtg accgtcaacc tgagtttaca caagttgact tggaaacgtc attcctttct
     4141 gaacaagaaa ttcaagacat cgttgaaggc atgattgcta aagtgatgaa agagacaaaa
     4201 gaaatagatg taaccctacc attcccacgt atgagctacg atgtagcaat gaatagctac
     4261 ggctctgata aaccagacac acgttttgaa atgcttttac aagacttgac agtaactgtc
     4321 aaaggaaatg acttcaaggt tttctcagaa gcaccagctg tcaaggcgat tgtggtgaag
     4381 ggaaatgcag atcgatactc tcgtaaagat attgacaaat taactgagtt tgccaaacaa
     4441 tttggtgcta aaggccttgc ctgggttaag gtgacagatg gtcaattggc aggcccagta
     4501 gccaagttct tgacagctat tgagacagag ttgtcaagcc aattaaaact tgctgagaat
     4561 gatttggttt tatttgtagc ggatactctt gaggttgcta ataatacatt aggtgccctt
     4621 cgtaatcgta ttgccaagga ccttgacatg attgatcagt cgcagtttaa tttcctttgg
     4681 gtggttgatt ggccaatgtt tgagtggtca gaagaagaag gacgttatat gtcagctcat
     4741 caccccttca ctcttccaac gccagaatct gctcatgagt tagagggtga tttggctaag
     4801 gtacgggcta ttgcttacga catcgtttta aatggttacg agcttggtgg tggtagcctg
     4861 cgtatcaatc aaaaagagat gcaagagcgc atgttcaagg ctcttggctt taccgctgat
     4921 gaagctaacg accaatttgg tttcttacta gaagcgatgg actacggctt tcctcctcat
     4981 ggtggcttgg caattggtct tgaccgtttt gtcatgttgc ttgctggaaa agacaatatt
     5041 cgagaagtta ttgccttccc taaaaataac aaagcctctg atccaatgac gcaagcacct
     5101 agtcttgttt ctgaaaacca attagaagaa ctcagtcttc aaatagaaag tcatgattaa
     5161 gaaaacaacc tataagaaaa aagttaaata tgtcattagt agaggggcca agaaagttgg
     5221 cctactccac gctctaagaa gtatttcaag agaaaaatat gcagagaaga tttcggcttc
     5281 tctgctttat ggcattctct ctagtattgc tgtgaatttt ttcttccagc ctgggcatgt
     5341 ttattcaagt ggagcaactg gtctagcaca ggttttttca gctcttagtc atcgtctttt
     5401 aggctatgat tttcccatcg cctttgcgtt ttatttgatt aatattcctt tgcttatttt
     5461 agcttggtat aaaattgggc atcaatttac catttttacc tttatcacag tcagcatgag
     5521 ttctttcttt attcaaatca tgcctcaagt gacgctgacg actgatcctc ttatcaatgc
     5581 tatttttggt ggtttggtta tgggaatggg aattggtaca ggtctcaaat cacgtatctc
     5641 tagtgggggg actgatattg tcagtttgac ccttagaaaa cgaacaggca aggatgtggg
     5701 cagtctctca ttgatggtta atggtgcaat tttagccttt gcagggattt tatttggctg
     5761 gcagtacgcc ctttattcta tggtctctat ctttgtatca agtcgtgtta cggatgccat
     5821 tttcaccaag caaaagaaaa tgcaggcaac tattgttacc agccatccag agcgtgtgat
     5881 tcatatgatc cataaacgtc tgcatcgcgg agtgaccagt atcaacgacg cagaagggac
     5941 ttacaagcat gaacaaaaag cagttttgat taccattttg acatgtgaag aatacccaga
     6001 attcaaatgg ctgatgttaa aaacagaccc acaagccttt gtttcagtgg ctgagaatgt
     6061 tagaattatc ggtcgttttg tggaagatga ttaagaaaaa cacaggaggt gtcatgtcaa
     6121 ttgaccaacg tttacagtta aagaaatttt gctttattat tttaggagct gctatctacg
     6181 cctttgcttt tgtttatttt tacatggcta atcgcattgc agccaatggt ttagctggtc
     6241 tcaccctagt tggtaaggca ctttttgaga ttgatccttc tttgataggc tatttgatta
     6301 atttgccttt ggtcttgctg ggagctagat gttttgggaa aagagccatg gtttacacag
     6361 taacgggtat tttatcactt tacttttttg tgtggctgtt tcaacgcttt cctttggtcg
     6421 ttgacttgga ccatgacaat ttagtcgttt ccttgatggc tggaattatt ggtggtctag
     6481 gtggcggtct tgtgtttcgc aatggtggaa ccatcggtgg agcagatatt attgctaagt
     6541 tattagaaga caagttagga ctacagctga atcaagctct tttaggcatt gatctttttg
     6601 tcatgattgt ctctttgact tatatttccc tgccgcaaat gatgtatgct ctgattgcta
     6661 gctttatgta tggtcaaatt gttcgcttgg tacaacaagg tggttattca gcccgtggca
     6721 tttttatcgt ttcagaacaa gcggagaaaa ttgcccggtt catcatggac gaattgggaa
     6781 gaggagttac ttatctaaca ggtgaaggag cctactctgg tcgccgtaaa aaagtgattt
     6841 atgtggcttt ggggcgaaga gatattaggg aattaaaagc ttttttaact caggttgatc
     6901 ccaatgcctt tatcacttat tttgatgtta atgaagtcaa tagcccagaa tttttaacta
     6961 ttaaaagcaa ataccataaa aagagaaaat aaatgagcat tagagttgcc tgcgaagaat
     7021 agtgaggata aaggatggca catcacgata aattgactaa actactaaaa ctgtttttga
     7081 ttgccctagg tgtagccatt tataccttcg gttttgttaa ttttaacatg gctaatgctt
     7141 tggctgaagg tggtgtggca ggaataacct tgattttaca cgcgcatttt gggattaatc
     7201 ctgcttactc ttctctctta tttaatcttc cactttttat tttaggggca aagatttttg
     7261 ggaagcgttc tttggctcta accatctatg gaacagttct gatgtccgct tttatctgga
     7321 tgtggcaaaa agttcctatc gaacttggct tggaaaatga catgatgttg gtggctgtag
     7381 tggcaggtct cttttcaggg attgggagtg gcattgtttt tcgctatggt gcaaccacag
     7441 gtggaacgga tattattggt cgcattgcag aagaaaaatt tggagctaag ctaggtcaaa
     7501 ctttgctttt ggtggatgct cttgtattga cagcttcgtt gacttatgtg gatttaaaac
     7561 acatgcttta cactttggtg gcaagctttg tttttagcca aatgattagt gtggttcaaa
     7621 atggtggtta cactatccgt ggaatgatta ttattaccaa gcattcagag gctgccgctc
     7681 aagctatcct aaccgaaatc aatcgtggag tgacttactt gaaaggtcaa ggagcttact
     7741 ctggtaatga ttacaatatc atgtatgtga ccttgaaccc aactgaagtt cgggaagtca
     7801 aacgtatttt agctggtttg gatccagatg cctttatctc cattattgat gtggacgaag
     7861 ttattagctc tgattttaaa attcgccgaa gaaattatga taaataaaaa tacaggtcaa
     7921 acagcctgta tttttatttt ggtcccccac ttaagaaact accaagcata atagctgata
     7981 tagcagttcc cttgtaatgg ctagcttttg tttttaccaa gcgactaaga tcaaatatgc
     8041 ttttaggagc atcaaattga tgtgttaaga tgtattgatt gataagtgag tcaatttttg
     8101 tagctaggag tgagctcatc ccctcttttt caagttgact ggcgagctcg aagatcgctt
     8161 gacgaaaata atcatctgct tgtattactt gttcgctata aactttacta agagcatcta
     8221 atatttcttt ttctgatggc atggcattct cctgtaacat attgtgctat tattataata
     8281 taaaaaacta agactgtcat aaatgtgaca gccttagtaa tgttattact tacatttcat
     8341 ttggtgcgtc aacaccgagt aagcgaagcg cttctttaag aacagtagca gttgcatagc
     8401 agagagctaa gcggttatca cgttctgagt tgtcgtcgag aatgcgagtg tgagcataat
     8461 atttgttaaa gctttgggct aagttaatag caaatttagc cataatagaa ggctcaaagt
     8521 tatctgatgt tcgcttgata atgcgtggga aatcttggat aagtttgata atttcccagc
     8581 tttcagcatc agctaaactg taagtggtgg ttgcagatgg cgtaaagtca gctttacgta
     8641 gaatagattg gatacgagcg tgagcgtatt gaacataagg tccagtttct ccttcaaagg
     8701 aaaccatggc ctcaaggtca aagtcatacc cattcatacg atctgttttg agatcgtaaa
     8761 acttgatggc accgacacca acagcgtgtg caacagcttc tttgtcagca aggtttggat
     8821 ttttcgcttc aatttgagaa gctgcacggt taatggcttc agcaaccgta ggttcaagaa
     8881 gaataacatt acctttacgt gttgataatt tagcaccgcc ttttgtgaca agtccaaaag
     8941 caacgtgtgt catatcatct gaccaatcgt aacccatttc ttttagcact gcttttaatt
     9001 gtttgaagtg agcagcttgc tcattaccaa caacataaac ggatttagca aagtcataag
     9061 tgcgtttacg gtaaagtgcg gcagctaggt cacgggtaat gtaaagagta gcgccatctg
     9121 attttttgat caaggcaggg tgttcaatac catatttttc aagattaaca acttgggcac
     9181 ctttagattc tacaagaagg tttttagctt ctaataagtc aagaacctca tccattttgt
     9241 cattatagaa ggcctcaccg ttgtaactat cgaaggtgac atgtaattgg tcataaagtc
     9301 ggttgaactc aagtaaactt tcatcacgga accattgcca taattcagta gcttctttgt
     9361 cgccatcttc caatttacgg aaccattcac gagcttcttc atcaacggtg ggatcagttt
     9421 cagcttcagc attgatacga acgtaaagtt ttaagagttc atcaatagga tgtgcctgaa
     9481 cagcagcttc atctccccat tttttgtagg caacaatgag cataccaaat tgtttacccc
     9541 agtcaccaag gtgattgatt tttacaggtt tatagcccat tttggcaaaa atgtgagcga
     9601 gactatcacc aataactgtt gagcggaggt gaccgattga aaatggctta gcgatgtttg
     9661 ggcttgacat gtcaatagca acatttcgcc cttgaccttc gtcttgctga gcatagtctg
     9721 aacctgctgt aatcacttgt tcaaggacct gtgaggaaat cttagcttta tcaaggaaaa
     9781 agttgatgta agggccgaca gctacgactt tttcaaattg gctctcatca atctgttcag
     9841 ctaactcgct agcaatcatt tgaggggctt tgcgaaggac ttttgctaag ctaaaagctg
     9901 gaaaggcaag gtctcccatg tcagaatttt ttggggtttc aagcaagtta aaaatagcat
     9961 cttgttctaa ttctggaaca actttagcta tttcacttgc gattagagtt ttagtatcca
    10021 tacagcgtct cctaaaaatt gtttgtcacc ctattctaac atatttcttt aaaataaacg
    10081 aggagaggat tgataaatta tactgaaact ttcaaggaaa tgaattgctt gaggttgttg
    10141 tttttctgaa aatgttataa aaagagaaat gaggtttctt ttcaacagtt ttcaagtgaa
    10201 attctagttg aatttgataa aaaattgctg aaaatagctg ggcgaagatg aaataataca
    10261 ctttttttgt tataatttga gaggataatt atacaattgg ggtgatatat catgaataaa
    10321 atggagcgtc aacagcagat taagcgaatc attcaagcag agcatattgg cacacaagaa
    10381 gatattaaaa atcaccttca aaaagaaggc attgtggtca cccaagcaac tctatctcgg
    10441 gacttaagag aaattggcct tttgaaattg agagatgaac aaggcaaact ctattatagt
    10501 ttgtctgaac ccgttgcaac accatttagt cctgaggtgc gtttttatgt tttgaaagtt
    10561 gatcgagcag gttttatgct ggttctccat accaatttag gagaggctga tgttctagct
    10621 aatttgattg acaatgatgc cattgaagat attctaggaa ccattgctgg tgcggatacc
    10681 ttgcttgtga tttgcagaga cgaagagatt gctaaacgct ttgaaaaaga tttggctgca
    10741 ggcctatgac tagctattaa gagtctttga cacaattgat tcttgctatc catgaattct
    10801 agtatcgttg cttataaaga agtgcagcaa tagctgtcta agctccatga ttttaaaaag
    10861 taagcttatc agatgaaacg ctatcaacaa gatgccctgc ttttcaaaaa aaatagataa
    10921 agaaaaggct gcgacagtat ctgcaagcag ggcaaaagaa ctagaagata ggctcagtca
    10981 tcagccatta attgatgatt atcgagaaaa gatgcaagat gcaagtgatg tgattcagta
    11041 tatcaccaaa cgtatagaag atcagttaaa caaggagtta acaaatggca aaaactaaca
    11101 tttctcctgg aatgcaacag tatctggaca tcaaaaaaga ttatccagat gcttttttgc
    11161 tttttaggat gggtgacttt tatgaattat tttacgagga cgctgtcaaa gcagcacaac
    11221 tcttagaaat tggtttgacc agtcgcaaca agaatgcgga aaatccaatt cctatggcag
    11281 gcgtgccaca tcattctgcc caacaataca ttgatgtgtt aattgagttg ggttacaagg
    11341 ttgctgtcgc agaacaaatg gaagacccaa agcaagctgt tggggtggtg aagcgtgagg
    11401 tcgttcaagt cataactcct ggaacggttg tggattcagc taagccagat agcgccaata
    11461 actttttggt agctgttgac tttgatggtt gccgttatgg attggcttat atggatgtat
    11521 ccacaggtga attttgcgtg acagatttgg cggactttac gagtgttcgt agcgaaatcc
    11581 aaaacctcaa ggcgaaagaa gtcttactag gttttgattt atctgaagaa gaacagacga
    11641 ttttggtcaa gcagatgaat ttgctgcttt cttatgaaga aacggtctat gaagataaat
    11701 ctttaattga cggccaattg acaacggtag aactgacagc ggcaggaaaa ctcttgcaat
    11761 acgttcacaa aacacaaatg cgagaactca gccacttgca agcattggtt cactatgaga
    11821 tcaaggatta tttgcagatg tcgtatgcca ctaagtcaag tttagatttg gtagaaaatg
    11881 ctaggactaa taaaaaacat ggaagtctct attggctgtt agatgaaacc aagacagcta
    11941 tggggatgag gcttttgcgc tcatggattg atcgaccttt ggtttctaaa gaagctattt
    12001 tagagcgtca agaaattatt caagtttttc tgaatgcttt tattgagcga accgatttaa
    12061 gcaatagttt aaaaggtgtt tacgacatcg aacgcttatc tagtcgcgtg tcttttggca
    12121 aggcaaatcc gaaagattta cttcaattgg ggcatacctt agctcaagtg ccttatatca
    12181 aagctatctt agagtctttt gacagtcctt gtgttgacaa acttgtcaat gatattgaca
    12241 gtttgcctga gttggaatac ttgattagaa cagccattga tccagatgca ccagcaacta
    12301 ttagtgaagg aagtattatc cgcaatggtt ttgatgagcg cttggaccat tatcgtaaag
    12361 taatgcgaga ggggacaggc tggattgcgg atattgaggc caaagagcgt caagcaagtg
    12421 gcattaataa cctaaaaatt gattacaata aaaaagatgg ttattatttt cacgttacga
    12481 attcaaatct tagcttagtt cccgagcatt ttttcagaaa ggcaacttta aaaaattctg
    12541 aacgttatgg aacagcagaa ttggctaaga ttgaaggtca gatgttagag gctagggaag
    12601 agtcatctag tttagaatac gatattttta tgtgtattcg agctcaagtt gaaacctata
    12661 ttaatcgttt acagaaactg gctaaaattt tggcaacggt agatgttttg caaagtttag
    12721 cagtcgttgc tgaaaccaat cattatatcc ggccgcagtt caatgataat catgtgatta
    12781 caattcaaga aggtcgtcac gcggttgttg aaaaggttat gggagtgcag gaatacattc
    12841 ccaatagtat ctcttttgac caacagacca gtattcagct gattacaggt ccaaatatga
    12901 gtggtaagtc gacttatatg agacagctgg ccttaacggt tatcatggcc cagatgggtt
    12961 catttgtggc tgctgatcat gttgatttac ctttatttga tgcgattttt acgcgtattg
    13021 gggctgctga tgatttgatt tctgggcaat caacctttat ggtggagatg atggaagcaa
    13081 accaagcaat caaacgcgca agtgacaact ctcttattct atttgatgaa ctgggacgag
    13141 gcacggcaac ttatgatggt atggctttag cccaggcaat tattgaatat atccatgata
    13201 gagttggtgc taagaccata tttgcaacgc attatcatga attgacagac ttgtcaacta
    13261 acttgacaag tctagtcaat gttcatgtag caacgcttga aaaagatggc gatgttacct
    13321 tccttcataa gattgctgag ggaccggcgg ataaatctta cggtattcat gtggcaaaaa
    13381 tagcaggact gccaaaatcc ctattaaaga gagcagacga agttctgacc cgtttagaaa
    13441 cacagtcacg atctactgag ataatatcag tcccttcaca agttgagtca agcagcgctg
    13501 ttagacaggg gcaattatcc ctttttggtg atgaagagaa agctcatgag attaggcaag
    13561 cactggaagt tattgatgtc atgaacatga ccccgcttca agcaatgaca actctttacg
    13621 aattgaaaaa gttgttatag tctttcagct gaaaatgaaa aagatgcttt ctatattgaa
    13681 ggcatctttt tgttctgtca aaaacggtcc gaggccttcg gctatttatt aagtgtgtta
    13741 taatagtcca taagaatgcg aggaaattat gacaaacatt attgaattac cggaagttct
    13801 cgccaaccaa attgcagctg gtgaagttgt agaaaggcca gcgagtgttg tcaaggaatt
    13861 ggttgagaat gctattgatg ctaaaagtag ccagattacc gttgaaattg aagagtctgg
    13921 ccttaagatg atacaggtta cagacaacgg tgagggaatg tctcatgaag atttaccttt
    13981 aagtctgcgt cgccacgcta ctagtaaaat taagagtcag agtgatttgt ttagaattag
    14041 aacacttggc ttccgtggag aggctttacc gtctgttgcc tctatcagta aaatcacgat
    14101 aaaaacagca acaaaagaag tcactcacgg ttctcttctt atagctactg gtggggaaat
    14161 tgagacactt gaagcgatct caactcctac tggaaccaaa attaaggttg aaaacctttt
    14221 ttacaacacg cctgctcgtc tcaaatatat gaaaagttta caggcagaat tagctcacat
    14281 tgtagatgtg gtcaaccggt tgagtttggc acatccagaa gttgctttca cactgattag
    14341 tgatggtcgc caattaactc agacatcagg aactggcgat ttacgccaag caattgcagg
    14401 gatttatggt ttgaatacta ccaaaaaaat gctggctatc tctaatgctg atttggattt
    14461 tgaagtttcc ggctatgtta gcttgccaga gttgacacgc gccaaccgta attacatgac
    14521 gattttggtt aatgggcgtt acatcaagaa tttcttgctt aatcgagcaa ttcttgatgg
    14581 ttacggttct aagctcatgg ttggacgttt cccaattgtt gtgattgata ttcagattga
    14641 tccctatttg gccgatgtca atgttcaccc cacaaaacaa gaggttcgta tttcaaaaga
    14701 gcgtgaatta atggctttaa ttagcacggc tatttcagag agtcttaagg aacaagattt
    14761 gattccagat gctctggaaa atttagccaa atcaagcaca cgacatttct ctaaaccaga
    14821 gcaaacacaa cttcctttac agtctagggg actttattat gatcctcaaa agaatgactt
    14881 ttttgtcaaa gagtcggctg tctcggaaaa aatacctgaa actgattttt attccggcgc
    14941 tgttgacaac agtgtaaagg ttgaaaaagt agagctgtta ccccactcag aagaagttat
    15001 aggaccttct tcggttaaac acgcaagtcg tccccagaat acctttaccg agactgatca
    15061 tcctaatctt gacttaaaaa acagacaaaa gttatctcaa atgctaaccc gtttggaaaa
    15121 tgaagggcaa tcagtgtttc ccgaattgga ttattttggt cagatgcacg gaacttatct
    15181 ctttgctcag ggaaaagatg gtttatttat aattgaccaa cacgctgccc aggagcgggt
    15241 taaatacgaa tattatcgtg ataagatagg tgaggttgac agtagtttac agcaattgtt
    15301 ggtgccctat ttgtttgagt tttctggttc tgatttcatt aacttacagg agaaaatggc
    15361 actcttaaat gaagttggta tcttcttaga agtttatggg cacaatactt tcattttgag
    15421 ggagcatcct atctggatga aagaagaaga gattgcatct ggtgtctatg aaatgtgtga
    15481 catgttactt ctaaccaatg aggtatctat taaaacttac cgagcagagt tagctattat
    15541 gatgagttgt aaacgatcta tcaaggcaaa tcatagcttg gatgattatt cagcaagaaa
    15601 tctgctactg caattggccc aatgtcaaaa tccttataac tgccctcatg gtagacccgt
    15661 attgattaat tttagtaagg cagatatgga aaaaatgttc cgtcgaattc aagaaaatca
    15721 cactagcctg cgagagctag gaaaatatta gagaggatga gatgcaagag tttttaaacc
    15781 ttcctaagca gattcagctg aggcaactgg tacgctttgt gaccattacc ttaggcagta
    15841 gtatctttcc ctttatggcc atgtattata cgacttactt tggtacgttt tggacaggcc
    15901 tcttaatgat gattaccagt ttgatgggat ttgttggaac tttatacggt gggcatctgt
    15961 cagatgctct tggtcgcaaa aaagtcatta tgattgggtc agtaggaaca acactaggct
    16021 ggtttctgac tattttagct aatttgccta atgcggctat tccttggtta acctttgcgg
    16081 gtattttatt ggtagagatt gcttctagtt tttatggtcc tgcctatgaa gctatgttga
    16141 ttgatttgac tgatgagagt aatcgtcgat ttgtttacac catcaattat tggtttatca
    16201 atattgccgt catgtttggt gcagggctat ctgggctttt ttatgaccat cattttttag
    16261 ccttgttagt agccttatta ctcgtcaatg tactttgttt tggcgttgct tactactgtt
    16321 ttgatgaaac tagaccagaa acacacgctt ttgaccatgg taaaggatta ctggcgagtt
    16381 ttcagaacta ccgtcaggtg tttcatgatc gtgcctttgt cttgtttacc ttaggtgcca
    16441 tcttttctgg tagtatctgg atgcagatgg ataactatgt gccagtccat ttgaaactgt
    16501 attttcagcc aacggctgtg ttaggtttcc aagtaactag ttctaaaatg ttatcattaa
    16561 tggttttaac taatacattg ctgattgtcc ttttcatgac agtagtaaat aaattaacgg
    16621 aaaaatggaa actattacct cagcttgtgg ttggttcttt actatttact ctagggatgc
    16681 tcttgtcatt tacctttacg cagttctatg ctatctggtt atcagttgtt ttgttaactt
    16741 ttggggaaat gataaatgtt tctgctagtc aagtcctacg tgctgatatg atggatcatt
    16801 cccaaatagg atcttataca ggttttgtgt caatggcaca acccctaggt gctattttgg
    16861 ctagtctact agtatctgtc agccatttta caggtccttt aggcgtgcaa tgcttatttg
    16921 cagtcattgc tttgctaggg atttatttta cggttgtttc tgcaaaaatg aaaaaggtgt
    16981 aatatgtacg attatattaa aggtcaattg accaaaatta cggcaaaata cattgtcgtt
    17041 gaagctaatg gattgggcta catgattaat gttgccaatc cttatagctt tacagatagt
    17101 gtcaaccaat tggtaaccat ttatctgcat caagtgattc gtgaggatgc tcacctgttg
    17161 tttgggtttc acacggaaga tgaaaaagat gtttttctga aattaatttc tgtatcaggt
    17221 attggtccga caacagctct tgctattgtg gcagttgatg ataatgaggg acttgtgaat
    17281 gccattgata acagtgacat taagtaccta atgaaatttc ctaaaattgg taaaaaaaca
    17341 gcgcagcaaa tggttcttga cttagctggc aaatttgtgg aggctccaca agagactggt
    17401 cataccaaag cgcgcagcaa taaagcaggc aatactcaac tggacgaagc gattgaagcc
    17461 ctccttgctc tcggttataa agcaaaagag ctgaaaaaaa ttcgtgcctt ctttgagggg
    17521 acctctgaga cggcagagca atacatcaaa tcagcattga aactgttaat gaaagggtag
    17581 gtgtttcaca tgaaacgttg ctcatgggtt cctaaggata accagcttta ctgtgattac
    17641 cacgatttag aatggggtca acccctggat gatgatagag atttttttga attgctgtgc
    17701 cttgaaagtt accagtcagg cttatcctgg ctgactgttt tgaaaaaacg ccaagctttc
    17761 agaactgttt ttcatcatta cgacattgca tcggttgcaa cctttacctc ggaggaaatg
    17821 gctgatgctt tagagaatcc atccattatc cgccacaagc taaagctagc agcaactgtc
    17881 aacaacgcta tcgcagtaca gaaaattcaa aaagagtttg gtagtttttc aacttatctc
    17941 tggaattttg ttgggggaaa gccaattaac aaccttgtca atcaagagaa tcttgttcct
    18001 gctcaaacag aattatctat tcgcttagct aaggatttaa agaaaagagg atttaaattt
    18061 ttagggccta caacggttta ttcttttatg caagcatcgg gtctggtaaa tgaccatgaa
    18121 gaggcttgtg ttttcaatag aatcttctaa ttcttcgtga attggaagat tttttgataa
    18181 aatgagatca gattacgaat agataagtag gtagggaaat gaaagctgaa ctgattgcag
    18241 taggtaccga aattttgact ggtcaaattg tgaataccaa tgctcaattt ctgtcggaaa
    18301 aaatggcaga gctaggtatt gatgtctatt ttcaaacggc tgttggggac aacgaggagc
    18361 gtttactttc agtgattaca actgctagtc agcgtagtaa cttggtaatt ttatgtggtg
    18421 gccttggtcc aacgaaagat gatttaacca aacaaacttt agcaaagtac cttaggaaag
    18481 acttggttta tgatgagcaa gcttgtcaga aactagatga cttttttgct aagcgcaagc
    18541 cttcatcacg gacaccaaat aatgagcgac aggcacaagt gattgaaggg tcaatccctt
    18601 tgccaaataa aactggtctt gcggttggtg ggttcatcac agtcgatggt attagttatg
    18661 ttgtcttacc gggtcctcca agtgaattga agccgatggt aaatgaagaa ttggtaccac
    18721 ttctgtcaaa acaatacagt acattgtatt caaaggtact acgctttttt ggtattgggg
    18781 aaagtcagtt ggtaacagtc ttgtcagatt ttattgagaa tcaaactgat ccaaccattg
    18841 ctccgtatgc taagactggc gaagtgactc ttcgcttatc aacaaaaact gaaaaccaag
    18901 ctctggcaga taaaaagtta ggtcagctag aagcgcagct actatcccga aaaactcttg
    18961 aaggtcaacc cttagctgat gtcttttatg gctatgggga ggataattcc ttagcgcgtg
    19021 agacatttga gctcttagta aaatatgata agacaattac agcagcagaa agtctaaccg
    19081 cgggattatt tcagtcaact ttggcgagtt ttccaggagc ttctcaagta ttcaatggag
    19141 gctttgtgac ttatagcatg gaagaaaaag cgaaaatgct aggccttcct ttagaggagt
    19201 tgaaatcgca tggcgttgtt agtgcttata cggccgaggg gatggcggag caagcaaggt
    19261 tattgactgg tgctgatatt ggggtaagtt taacaggtgt tgccggacca gatatgttgg
    19321 aggaacagcc tgcaggtaca gttttcattg gtcttgccac tcaaaataag gtagaatcaa
    19381 taaaggtttt gattagcggg cgaagtcgtt tggatgtgcg ctatatcgct actttacatg
    19441 cttttaatat ggtccgtaaa actttattaa aacttgagaa tttgctataa tgttatttag
    19501 tagctatagt aggatatggt ttacaattat gtagattaat cctgctgacg atggtttgtt
    19561 aaaaaagagg agaaagtatt ggcaaaaaaa ttaaaaaaga atgaagaaat cactaagaag
    19621 tttggtgacg aacgtcgcaa agcccttgat gatgctttga aaaatattga aaaagatttt
    19681 ggtaagggtg cagtgatgcg attaggagaa cgtgcagagc aaaaagttca ggttatgagt
    19741 tcaggaagtc tagctcttga tattgcgctt ggagctggtg gttatcctaa aggacgtatc
    19801 atcgaaatct atggtccaga gtcttccggt aaaacgactg tggctttaca tgctgtagca
    19861 caagctcaaa aagaaggtgg aatcgcagcc tttatcgatg ccgagcatgc gcttgatcca
    19921 gcttatgctg ctgcgcttgg ggttaatatt gatgaactcc tcttgtctca accagattct
    19981 ggagaacaag gacttgaaat tgcaggtaaa ttgattgatt ctggtgcggt tgatctggtt
    20041 gttgtcgatt cagtagcagc tttagtgcca cgtgctgaaa ttgatggtga tattggcgat
    20101 agccatgtcg gattgcaagc acgtatgatg agtcaggcca tgcgtaaatt atcagcttct
    20161 attaataaaa caaaaactat cgctatcttt attaaccaat tgcgtgaaaa agttggtgtg
    20221 atgtttggaa atcctgaaac aacaccaggt ggtcgagctt tgaaattcta tgcttctgtt
    20281 cggctggatg tgcgtggaac aactcaaatt aaaggaactg gtgaccaaaa agatagcagt
    20341 attggtaagg agaccaaaat caaggttgtt aaaaacaagg tcgctccgcc atttaaggta
    20401 gcagaagttg aaatcatgta tggggaaggt atttctcgta caggggagct tgtgaaaatt
    20461 gcttctgatt tggacattat ccaaaaagca ggtgcttggt tctcttataa tggtgagaag
    20521 attggccaag gttctgaaaa tgctaagcgt tatttggccg atcacccaga attgtttgat
    20581 gaaatcgacc ttaaagtacg tgttaaattt ggtttgcttg aagaaagcga agaagaatct
    20641 gctatggcag tagcatcaga agaaaccgat gatcttgctt tagatttaga taatggtatt
    20701 gaaattgaag attaaaaaga gcccttcagg gctttttaaa atgtattttt aaaggttagt
    20761 aggactactg ttcataggtg tgtgggtgaa tagaaaccat catttaaaaa gatgtgagag
    20821 agcagaact
//
LOCUS       DAXWIQ010000032        20732 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000032 DAXWIQ010000000
VERSION     DAXWIQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20732
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.032"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(115..1251)
                     /gene="dnaJ"
                     /locus_tag="ACGEUI_001218"
     CDS             complement(115..1251)
                     /gene="dnaJ"
                     /locus_tag="ACGEUI_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFF8166056.1"
                     /translation="MNNTEYYDRLGVSKDASQDDIKKAYRKMSKKYHPDINKEAGAEQ
                     KYKDVQEAYETLSDSQKRAAYDQYGAAGAQGGFGGGAGGFGGFDGGGFGGFEDIFSSF
                     FGGGGSRNPNAPRQGDDLQYRVNLSFEEAVFGVEKEVSYNREATCGTCLGSGAKPGTA
                     PVTCRKCHGSGVMTIDTQTPLGMMRRQVTCDICHGSGKEIKEPCQTCHGTGHEKQAHK
                     VSVKIPAGVETGQQIRLQGQGEAGFNGGPYGDLFVILNVLPSKQFERNGSTIYYNLDI
                     SFTQAALGDTVEIPTVHGDVEMAIPAGTQTGKTFRLKGKGAPKLRGGGQGDQHVTVNI
                     VTPTKLNDAQREALQAFAEASGEKMLHPKKKGFFDKVKDALEDI"
     gene            complement(1532..3358)
                     /gene="dnaK"
                     /locus_tag="ACGEUI_001219"
     CDS             complement(1532..3358)
                     /gene="dnaK"
                     /locus_tag="ACGEUI_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFF8166057.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPETVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVAEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIKSNDGLSEEEIDRMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKAAQESGNLDDMKAKLEALNEKAQ
                     ALAVKMYEQAAAAQQAAQGAEGAQANDSANNDDVVDGEFTEK"
     gene            complement(3539..4111)
                     /gene="grpE"
                     /locus_tag="ACGEUI_001220"
     CDS             complement(3539..4111)
                     /gene="grpE"
                     /locus_tag="ACGEUI_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFF8166058.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            complement(4114..5148)
                     /gene="hrcA"
                     /locus_tag="ACGEUI_001221"
     CDS             complement(4114..5148)
                     /gene="hrcA"
                     /locus_tag="ACGEUI_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015911815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFF8166059.1"
                     /translation="MITQRQNDILNLIVELFTQTHEPVGSKALQRTIDSSSATIRNDM
                     AKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQDIYHVIKAFDFEAFKLE
                     DMLQKASHILAEMTGYTSVILDVEPARQRLTGFDVVQLSNHDALAVMTLDESKPVTVQ
                     FAIPRNFLTRDLIAFKAIVEERLLDNSVIDIHYKLRTEIPQIVQKYFVTTDNVLQLFD
                     YVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLRQSLKEGEMASVQVADS
                     QEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLVNIIGKVLAAKLGDYYR
                     YLNSNHYEVH"
     gene            complement(5283..5852)
                     /locus_tag="ACGEUI_001222"
     CDS             complement(5283..5852)
                     /locus_tag="ACGEUI_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFF8166060.1"
                     /translation="MRKRLKFPYFLTLLACFLLLIVCPLLSSQRIASADKEVRVNYSQ
                     KQFITKMGKEVKPLAKYYGIRPSILIAQILLETHDGKTLLASKYHNLFSKKATPGQVA
                     ITLKSPKQTNQNVRYAIYKDDASAIRDYLRMLRQGKEVDKRLYRNLATEKGYKAPAKS
                     LQKYLHYTDKTYARRLIQVIESNDLTNYD"
     gene            complement(5849..6592)
                     /locus_tag="ACGEUI_001223"
     CDS             complement(5849..6592)
                     /locus_tag="ACGEUI_001223"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFF8166061.1"
                     /translation="MKYNSLNYLVQLLIVIVFLGGLYLFIKPEESVTPTQLNKKEIQQ
                     KDIKKTDRLRALPKVSVEDWELVLVNRDHITKEMSPELADINGISVDKRIEQATSDFL
                     AAAQAIDLQEHLISGYRSVDYQTELYQSYIKKEMANDPTLTQEAAEALVQTYSQPPGA
                     SEHHTGLAIDMSTVDTLNASDPSVAKAVQKIAPDYGFVLRFPEGKKTSTGVDYEDWHY
                     RYVGKASARYMAQHNLTLEEYIAALKEKR"
     gene            complement(6582..7289)
                     /locus_tag="ACGEUI_001224"
     CDS             complement(6582..7289)
                     /locus_tag="ACGEUI_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8166062.1"
                     /translation="MTKTRLYIARHGKTMFNTIGRAQGWSDTPLTKKGEEGIRELGLG
                     LKDATIPFKAAFSSDSGRTMQTIEIILRESENEFLPYTKDNRIREWCFGSLEGTYDSE
                     LFLGVLPRTKAFENRDNLRDVPYSELAESIVEVDTANWAEPWEVLRKRIWEGFEAIAL
                     SIQNAGGGNALVVSHGMTIGTFLWLIDPDRDKQYIDNGSVTVVEFDDGQFTIKTIGDM
                     SYRYRGREIIEERSDEI"
     gene            complement(7680..7919)
                     /locus_tag="ACGEUI_001225"
     CDS             complement(7680..7919)
                     /locus_tag="ACGEUI_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8166063.1"
                     /translation="MIWSLIVGAFIGMIAGKMTEKSQSMGCFTNIIAGLIGSAVGQRL
                     FGDWGIQLAGMAIFPSILGAAIVIVLVSAIFGKRY"
     gene            complement(8138..8353)
                     /locus_tag="ACGEUI_001226"
     CDS             complement(8138..8353)
                     /locus_tag="ACGEUI_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166064.1"
                     /translation="MIVADSNKLKYFVSEKKAVGLSQAAFLYVMLVTAYKKTIIYAKI
                     RLTGIALEPSGVSDGYLMNYHVCLHYY"
     gene            complement(8350..9789)
                     /gene="gatB"
                     /locus_tag="ACGEUI_001227"
     CDS             complement(8350..9789)
                     /gene="gatB"
                     /locus_tag="ACGEUI_001227"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFF8166065.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHKEMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIDIKLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNS
                     FSNVRKGLEFEVERQAKLLRSGGVIRQETRRYDEANKGTILMRVKEGAADYRYFPEPD
                     LPLYEIDDAWIDEMRAQLPQFPAQRRAKYEEELGLSAYDASQLTATKVLSDFFETAVS
                     LGGDAKQVSNWLQGEVAQFLNAEGKTIEEIALTPENLVEMIAIIADGTISSKMAKKVF
                     VHLAKNGGSARAYVEKAGLVQISDPAVLVPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELQKLRD"
     gene            complement(9789..11255)
                     /gene="gatA"
                     /locus_tag="ACGEUI_001228"
     CDS             complement(9789..11255)
                     /gene="gatA"
                     /locus_tag="ACGEUI_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFF8166066.1"
                     /translation="MSFNHKTIEELHDLLVAKEISATELTQATLEDIKSREEAVGSFI
                     TVSEEVALKQAAAIDAKGIDADNLMSGIPLAVKDNISTKEILTTAASKMLYNYEPIFN
                     ATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTV
                     KENAQLLNVIASSDVKDATSAPVRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKE
                     TVLASVKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRADDA
                     KNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFA
                     DYDLILGPTTPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVG
                     LQLIGPKYAEETIYQAAAAFEAVTDYHKQQPIIFGGDK"
     gene            complement(11255..11557)
                     /gene="gatC"
                     /locus_tag="ACGEUI_001229"
     CDS             complement(11255..11557)
                     /gene="gatC"
                     /locus_tag="ACGEUI_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFF8166067.1"
                     /translation="MKISEEEVRHVAKLSKLSFSESETTTFATTLSKIVDMVELLNEV
                     DTEGVAITTTMADKKNVMRQDVAEEGTDRALLFKNVPEKENHFIKVPAILDDGGDA"
     gene            complement(11789..12013)
                     /locus_tag="ACGEUI_001230"
     CDS             complement(11789..12013)
                     /locus_tag="ACGEUI_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HFF8166068.1"
                     /translation="MIRKGLAVSLARSVKPELKLGICGEHGGDPASIAFCHSQGLTYV
                     SCSPFRVPLTRLAAAQAAIKASGHSLTQDK"
     gene            complement(12552..13106)
                     /locus_tag="ACGEUI_001231"
     CDS             complement(12552..13106)
                     /locus_tag="ACGEUI_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFF8166069.1"
                     /translation="MRALISIDYTNDFVADDGKLSAGKSAQAIATKIAEVTKTAFDQG
                     DYIFFAIDCHDQNDSWHPESKLFAAHNIKGTTGRHLYGPLAEVYSYMKQHPRVFWIDK
                     RYYSAFSGTDLDIRLRERGITQLVLTGVLSDICVLHTAIDAYHLGYQLEIVKSAVASL
                     TKESYEWSLAHFEQVLGAKLINDY"
     gene            complement(13253..14035)
                     /gene="codY"
                     /locus_tag="ACGEUI_001232"
     CDS             complement(13253..14035)
                     /gene="codY"
                     /locus_tag="ACGEUI_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFF8166070.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLETELPYNTMASRLADIIDCNAC
                     IINGGGTLLGYAMKYKTNTDRVEEFFEAKQFPDTYVKAASRVYDTEANLSVENELTIF
                     PVESKDTYPGGLTTIAPIYGGGMRLGSLIIWRNDNEFSDDDLILVEISSTVVGIQLLN
                     LQTENLEDTIRKQTAVNMAINTLSYSEMKAVAAILGELDGNEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFAKLKEF"
     gene            complement(14253..15467)
                     /locus_tag="ACGEUI_001233"
     CDS             complement(14253..15467)
                     /locus_tag="ACGEUI_001233"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8166071.1"
                     /translation="MKIIEKSSKLEHVAYDIRGPVLDEANRMIASGEKILRLNTGNPA
                     AFGFEAPDEVIRDLIVNARLSEGYSDSKGIFSARKAIMQYCQLKGFPDVDIEDIYLGN
                     GVSELISISLQALLDNGDEVLVPMPDYPLWTACVSLGGGKAVHYLCDEEAGWYPDIAD
                     IKSKITSRTKAIVVINPNNPTGALYPKEILEDIVALAREHQLIIFADEIYDRLVMDGK
                     EHIAIASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKDHVKGYIEGLNMLANMRL
                     CSNVLAQQVVQTSLGGHQSVDALLLPGGRIFEQRNFIYEAINAIPGLSAVKPEAGLYL
                     FPKIDRQMYRIDDDEEFVLQLLKQEKVMLVHGRGFNWKDPDHFRIVYLPSVEELEDVQ
                     EKITRVLYQYRR"
     gene            15698..16150
                     /locus_tag="ACGEUI_001234"
     CDS             15698..16150
                     /locus_tag="ACGEUI_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8166072.1"
                     /translation="MSLKYKRILVAIDGSYESELAFNKGVNVALRNDATLLLVHVIDT
                     RALQSVATFDTYIYEKLEQEAKDVLDNFEKQAQIAGITNIKQIIEFGNPKNLLAHDIP
                     DRENADLIMVGATGLNTFERLLIGSSSEYIMRHAKIDLLVVRDSTKTL"
     gene            complement(16273..17661)
                     /locus_tag="ACGEUI_001235"
     CDS             complement(16273..17661)
                     /locus_tag="ACGEUI_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8166073.1"
                     /translation="MTVKAVFFDIDGTLLNDRKNIQKTTQKAIQQLKKQGIMVGLATG
                     RGPGFVQPFLENFGLDFAVTYNGQYILTRDKVLYQNQLPKSMIYKVIRYANEKKREIS
                     LGTASGLAGSRIIDMGTSPFGQVISSFVPKSWARTVEGSFKHLIRRIKPQSFRNLVTI
                     MREPIYQVVLVASQAETKKIQEKFPHIKITRSSPYSLDLISVDQSKIKGIERLGEMFG
                     FDLSEVMAFGDSDNDLEMLSGVGIGIAMGNAETVVKDGAHFTTDSNNNDGISKALAHY
                     GLIHFDIEKSFKSRDENFNKVKDFHRLMDSDTIETPRSYTISEAGYRSGFKVEEIVEF
                     LYAASKGDQQQFTQAIFDLHGAVDQAANKVQAKKHVETPLIGQVDALADLLYFTYGSF
                     VLMGVDPQPIFEAVHEANMAKIFPDGKAHFDPVTHKIQKPDYWQERHAPEVAIKKELD
                     KQLQKSLQRFDK"
     gene            17733..18698
                     /locus_tag="ACGEUI_001236"
     CDS             17733..18698
                     /locus_tag="ACGEUI_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFF8166074.1"
                     /translation="MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAAQDIQLT
                     VSDFLNLPSPHITPHHMLSIYHHIQERTDVFDGIVITHGTDTLEETAYFLDTMALPTN
                     IPVVLTGAMRSSNEVGSDGIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTH
                     TTNISTFQTPTHGPLGIIMKNDLLFFKTAEPRIRFDLRCISGTIPIIKAYAGMGDGSI
                     LSLLTPGSIQGLVIEALGAGNVPPLAVGEIEHLITLGIPVILVSRCFNGMAEPVYAYE
                     GGGAMLQEAGVMFVKELNAPKARLKLLIALNAGLTGQELKDYIEG"
     gene            complement(19047..19751)
                     /locus_tag="ACGEUI_001237"
     CDS             complement(19047..19751)
                     /locus_tag="ACGEUI_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8166075.1"
                     /translation="MISYLKADLFRIRQERLALFSCMLLVVIAVGVAYSFRKTPNQVI
                     MIQIINIITDFVTLFFLTPARIFFSDDYNYRTINNVVVKQQNRTLIIIYKLLVSFGLS
                     LLFISLTYLLSALAFHLFTGQAYYGIVIDRMIKQLPFYIAIIALCQFLFNILRKTYQS
                     ITAYILYVLLFDKVSATLANHFFKLDISPVLMFKHLSLSLGDKHLSSTSILVAFGLTL
                     CYLLGNVFLFSKRELK"
     gene            complement(19764..20666)
                     /locus_tag="ACGEUI_001238"
     CDS             complement(19764..20666)
                     /locus_tag="ACGEUI_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8166076.1"
                     /translation="MTQQSMLLSIEQVNKSYGKNQVLSDISFDIYKGEICGLVGQNGA
                     GKTTLMRILSGLIGKDSGQIKQLQPYRMGSIIESPTLYPNMTAHDNLYYAALQLRLAD
                     AKERIHEVLELIGLEKVSKKKKVKDCSLGMRQRLAIGLSILDFPEFLILDEPINGLDP
                     AGIKEMRQIILNLRDCYGITILISSHILSELDLVVDRYVIMHKGKVIKSMDKAELKAQ
                     VKVQIALHTSDDQLVKNRLLELGLRVETDGQMLLIKPTLSVMELIKIVLDLPVEIFDI
                     YHHQVSFEHYYLDLLGKDAASPLI"
BASE COUNT         6318 a         4373 c         3563 g         6478 t
ORIGIN      
        1 tttgacaaga gaaaaaaacg atatctataa aagaaaacgt tttctctaac tgttttgacg
       61 taaaaaaacg tataacttcg ggaaaattat cctgaaatta tacgtttgac atctttaaat
      121 atcctctaag gcatctttca ctttatcaaa aaaacctttt ttctttggat gtaacatttt
      181 ttcaccactc gcttctgcaa atgcttgcaa ggcttccctt tgagcgtcat tgagtttggt
      241 tggggtcaca atattaacgg taacatgttg atccccttga cctccaccac ggagttttgg
      301 tgctcctttg cctttgaggc ggaaggtttt acctgtttgt gtcccagcag ggatagccat
      361 ctcaacatcg ccatgaaccg ttggaatttc aacagtatct cctaaggctg cttgagtaaa
      421 agaaatatct aagttgtagt aaatagtgga accattgcgt tcaaattgct tacttggtaa
      481 gacattcaaa ataacaaata aatcaccata aggtccgcca ttaaagccag cttctccttg
      541 accttgtaaa cggatttgct gaccagtctc tacaccagct ggaatcttaa cagacacttt
      601 atgagcttgc ttttcatgac cagtaccgtg acaggtttga cagggttctt taatttcctt
      661 accactacca tgacaaatat cgcaggtgac ttgtcgacgc atcataccaa gtggtgtttg
      721 ggtgtcaatt gtcattacac ctgaaccatg gcacttacga caagttactg gagcggtacc
      781 aggttttgca cctgagccta aacaagtgcc gcaggttgct tcgcgattat agctaacttc
      841 tttttcaaca ccaaatacag cctcttcaaa agataaatta acacgatatt gcaaatcgtc
      901 accttgacga ggggcatttg ggtttcggct tccaccgcca ccaaagaagc ttgagaagat
      961 atcttcaaaa ccaccaaagc cacctccgtc aaaaccacca aagccgcctg ctccgccacc
     1021 aaagccgcct tgagcacctg cagcgccgta ttgatcataa gcagcacgct tttgtgaatc
     1081 acttaaggtt tcgtaagctt cttgaacatc cttatacttt tgctccgctc cagcttcttt
     1141 attgatatct ggatgatatt ttttagacat ttttcgataa gcttttttga tatcgtcctg
     1201 agaagcgtcc ttagaaaccc ccagacgatc gtaatattct gtattgttca taatgtaaga
     1261 taccaagctg taaaacgact taatgaaaaa taggaaatcc gacaaagata ctctcgtatc
     1321 taggaggtat ttctcttttt tcgcagagtc gtaggcgtgt tcaaatccgc aaaagagtct
     1381 ttacacgctt tagcctgtgt tcgaataatc gaacttgtat tcctttctta aaatattttg
     1441 acgtcagcat ttttacattt tatttagcca aaaacagagg tttggtacaa cctctgaatt
     1501 cggatattaa tgttactaga taactaaatc attacttttc tgtaaattcg ccatctacaa
     1561 catcatcatt atttgctgaa tcattagctt gtgcaccttc tgcaccttgt gctgcttgtt
     1621 gagctgctgc agcttgctcg tacattttaa cagccaaagc ttgcgctttt tcatttaatg
     1681 cttcaagttt agctttcatg tcgtcaaggt tgccagattc ttgcgcagct tttaactcgt
     1741 caagagctga ttgcgctgca tcgcgttctg tatcaaagcc tttaccttca gtttctttga
     1801 ttgttttttc agtagcaaag atagcttggt caacttcgtt tttaaggtca acttcttctt
     1861 tacgtttcgc atcggcttcg gcattagctt cagcgtcttt catcatgcga tcaatttctt
     1921 cttcagaaag tccgtcgttt gatttgataa cgatgtgttg ttccttttgc gtaccaaggt
     1981 ctttagcttt tacagaaaca ataccgtttt tatcgatatc aaatgttact tcaatttgtg
     2041 ggattccacg aggtgcagct gggatatcag tcaattggaa gcgaccaaga gtcttgttat
     2101 ctgctgccat tgggcgttca ccttgaagaa catggatatc aacagctggt tggttgtctg
     2161 ctgctgttga gaagacttgt gatttagatg ttgggatagt tgtattgcgg tcgatcaatt
     2221 tagtgaagac accacccatt gtttcaatac caagtgacaa tggtgttacg tcaagaagga
     2281 caacgtcttt cacatcccca gtgataacac caccttggat agcagcaccc atagcaacca
     2341 cttcatcagg gtttacagat ttatttggtt ctttaccagt ttcagctttt acagcttcga
     2401 caactgctgg gatacgagtt gatccaccaa caaggataac ttcatcaatt tctgacaatg
     2461 acaatcctgc atctgaaagg gcttgacgaa ctggagtttt cgtacgttct acaaggtcgc
     2521 gagtgagatc gtcaaattta gcacgagata agctcatctc caagtgaaga ggaccggcag
     2581 aaccagcagt gatgaacggt aatgaaattt gtgtttgtgt cacacctgaa agatcttttt
     2641 tagctttttc agcagcatct ttcaagcgtt gaagtgccat cttatcttgt gataagtcaa
     2701 tgccattttc tttcttaaat tcagccacta agaaatcaat aattttttgg tcaaagtcgt
     2761 caccaccaag tttgttatca cctgctgttg caagaacgtc gaagacacca tcacctaatt
     2821 caaggattga tacgtcaaat gtaccaccac caaggtcaaa aactaagatt ttttcatcct
     2881 tgtcagtctt atccatacca taagcaagtg cggctgctgt tggttcgtta acgatacgtt
     2941 ctacttcaag acctgcaatt ttaccagcgt ctttagttgc ctgacgttgt gcatcgttga
     3001 agtaagctgg aacagtaata actgcttttt ctactttttc tccaagatag tcttcagcat
     3061 aacctttaag gtattgaaga atcattgctg aaatttcttg aggagtatat tctttaccat
     3121 ttgcagaaac tttttcagaa gttcccattt tagatttaat agagattact gtttctgggt
     3181 ttgtcactgc ttgacgtttt gcagcatcac ccacgataat ttcaccattt ttgaatgata
     3241 ctactgaagg agttgtacga ttgccttctg ggttagcaat gatttttgat tcagtccctt
     3301 caagaactgc tactgctgag tttgttgtac ctaagtcaat accaataatt ttagacatgt
     3361 taatacctct tattttataa tactttatta ctttttattt caatttatct tagtaagcac
     3421 tcgaattatt ttacttccaa ttctaagcaa tcactatagt ttaatcagca agcgaaatag
     3481 cttctaactt gctatctagt ttactgtcat tacggacaaa aacgaaaact catttacctt
     3541 agttataaac aactaccatc gctggtctta gcagacgctc gtgcaattta tatccctttt
     3601 gaaagacttc tgcaatgcta tcggctggat gttcatcatc ggctgggagg gtctgaactg
     3661 ccatatgaaa gttgtggtca aacgagtcga cttctacttc ttctacgcct tcttccttta
     3721 gtgcttgaat aaggctatca cgagtcattt ctaaaccttt tttaacatca tctgtcaacc
     3781 cttcaacagc caaggcacgt tctagattgt caagcgatgg taagattgct tttgccagat
     3841 cctgagaacg atagcgttga agttgttgac gttcttcact agagcgacgc tgtatattct
     3901 gcatttctgc atgagctcgc aagtatttat tttcaaactc atcagcacgt tcgtttgcaa
     3961 gttctaactc tgatttttca ggagtttctt caataacttc ctcaactgtc tcttcagttt
     4021 cagtggcttc cacttcttct tgtaaatcgt cttttttaat ttcttctgac actgagtgcc
     4081 tccttttaaa taacttattg aatactgcca ttatcagtga acttcataat ggttagaatt
     4141 taggtatcga taataatcac ctaatttagc agccaatacc tttccaataa tgtttaccaa
     4201 actaacagac ctacgataat ccatatcaat aggaccaatc aaactcagga gtccaaaccc
     4261 acggtaagga atcaaaaatt tgtgagttaa aactgacaca tctgcaagtg cggcttcttg
     4321 actatctgcc acttggacag aagccatctc accttctttc agactttgcc tcaaagaaat
     4381 tgctacttgc tgttcattat ccaaaaactg atacgttgat agatctgaat acgttaaaga
     4441 atttaccttg ccagcaacaa ataccgtttc caaaaacagc tctgaaaaaa cataatcaaa
     4501 tagttgtaaa acattatccg tagtcacaaa atacttttga acgatctgag gaatttctgt
     4561 cctcaactta taatgaatat ctatgacact attatccagc aagcgctcct ctacgatagc
     4621 tttaaatgct atcaagtcac gtgtcaaaaa gtttcttgga atagcaaact gcactgtcac
     4681 tggcttagat tcatctaaag tcatgactgc tagagcatca tggttgctaa gctgaacaac
     4741 atcaaaccct gttaaacgtt gacgagctgg ctccacatct aaaataacgg atgtatagcc
     4801 cgtcatctcc gctaaaatgt gactagcttt ttgaagcatg tcttctaatt tgaatgcttc
     4861 aaaatcaaaa gcttttatga catgataaat atcttgctca tcaatactat ctagcctgag
     4921 ggagtgctct acaaaatact taaaaccagc tggacttggc atccgtccac tagaagtgtg
     4981 cgctttttcc aacaatccca acttctctag ttttgccata tcattacgaa tagtggcgct
     5041 actagaatca atcgttctct gtaaagcttt agaccctact ggttcatgag tttgtgtaaa
     5101 tagctcaacg attaaattca aaatatcatt ttgacgttgt gtaatcacaa accaaccact
     5161 ccctttcgtt tagcactcta tttagtagac tgctaattta taattctatt atactcttta
     5221 tgggagacat gtcaagaaaa aaactcaaaa aaattagcac tcttttataa agagcgctaa
     5281 tttcagtcat agtttgttaa atcgttactt tcaatgacct gaattagtcg cctagcatag
     5341 gttttatcag tataatgcaa atacttttgt aaacttttag ctggtgcttt ataccctttt
     5401 tctgtagcaa gattacgata caaacgctta tcgacttctt ttccctgccg aagcattcgt
     5461 aaataatctc taattgcact agcgtcatct ttataaatag catatctcac gttttggttg
     5521 gtttgtttag gggactttag ggtaatggcc acttgtcctg gagttgcttt cttgctaaaa
     5581 agattatgat acttagacgc tagtaatgtt tttccatcat gggtttccaa aagaatttga
     5641 gcaatcaaaa tagatggtct aatgccataa tattttgcca atggtttaac ttctttaccc
     5701 atttttgtaa taaattgttt ttggctgtag ttcaccctta cttctttatc tgcactagct
     5761 atcctttggc tactcagtaa gggacaaaca atcagcaata aaaagcaagc tagaagcgtt
     5821 aaaaaatacg gaaactttag tcgttttctc atcgtttctc ctttaaagca gcaatgtact
     5881 cttccaacgt taggttgtgc tgagccatat aacgagcaga cgccttgcct acatagcggt
     5941 aatgccaatc ctcataatca acccctgtac tagtcttttt tccttctgga aagcgcaaga
     6001 caaacccata atcaggtgca attttttgaa ctgctttagc tactgatgga tcactggcat
     6061 ttaaagtatc tacagtgctc atgtcaatag caagaccagt gtgatgttca ctagcccctg
     6121 gcggctgtga ataggtttgc accaaagcct ccgcagcttc ttgtgtcaat gttggatcgt
     6181 tagccatctc ttttttaata taagattggt aaagctctgt ttggtagtct acggaacggt
     6241 acccagagat aagatgttct tgcaagtcga tagcttgtgc tgctgctaaa aagtcactag
     6301 tggcttgctc aatgcgttta tccacagaaa taccattaat gtcagctaat tcaggactca
     6361 tttcttttgt aatgtgatcg cggtttacta aaaccaactc ccaatcttca acagatacct
     6421 taggtaaagc tcgtaaacgg tccgtttttt tgatatcttt ttgttggatt tcttttttat
     6481 tcaactgagt tggtgttact gactcttctg gttttataaa gaggtaaagc cctcctaaaa
     6541 aaacaataac aattagcaat tgcactaaat agtttaacga attatatttc atctgaccgt
     6601 tcttctatta tttcacgacc gcggtagcgg taactcatat ctccaatagt ctttatcgta
     6661 aattggccat catcaaattc aacaactgta acgctcccat tatcaatata ctgtttgtct
     6721 cgatctggat caatcaacca cagaaacgtt ccaattgtca taccatggct aaccacaaga
     6781 gcatttccgc cgccagcatt ttgaatagac aaggcaatag cttcaaaccc ctcccaaatg
     6841 cgcttacgaa gaacttccca tggttctgcc caattagcgg tatctacttc cacgatgctt
     6901 tctgccagtt cagaataggg aacatcacgt aggttatcac gattttcaaa ggcttttgtt
     6961 cttggaagga cacctagaaa taattctgaa tcataagtcc cttctagact cccaaaacac
     7021 cactcacgaa tacgattatc tttagtataa ggcaaaaatt cgttttctga ttctcttaaa
     7081 ataatttcta ttgtctgcat ggttcgacct gaatcacttg aaaacgcagc cttaaatggt
     7141 atagttgcat cttttaaacc taggccaagt tctcgaatac cttcttctcc tttttttgta
     7201 agaggtgtgt cactccaacc ctgagcgcga ccaatggtat taaacatcgt ttttccatgt
     7261 ctggcaatat acaaccttgt ttttgtcatt tcaacctcct aaactgtctt attaattatt
     7321 atagcattta aagaaaacac ttgcaaaaca cttcctgatg gctatttgag atcaaataga
     7381 ccactgactt attgtctatt ttattaaaaa caatgctatt gctacaattc aaatcatcac
     7441 ataaatttcc aaacgattac atttgtgtat ttcgctcaaa tatatataac aacagaccca
     7501 cactacaact aaactgacgt tacaaaatga tgtcaaaaca gtaacaaaat ctaactattt
     7561 gtcaagaaaa cgatattaaa ttttcacaca actatcttat aatgaggatg caaccaataa
     7621 aaagataaga cattttatgt tgcaaaaagg ctgcttggaa acttccatgc agcctttttt
     7681 taataccttt ttccaaaaat agctgaaaca agtacaatta caattgctgc tcccaaaatg
     7741 gacgggaaaa tagccatacc tgccaactga attccccaat ccccaaaaag cctttgccca
     7801 acagctgacc ctatgagacc tgcgataata ttagtaaaac aacccatgga ctggcttttt
     7861 tcagtcattt tacctgctat catgccgata aaggcaccta caattaaaga ccatatcatc
     7921 gttaacacct cctttcttgt aaaatccttt tagaagaact gctatccatt aaatgttctt
     7981 aactttagtt gattccttta ccttcttctt tagtaagtgt ttccgtgcct gagtaaaata
     8041 gaaaaaacaa ttccttctat taagatagat agaccgagaa cgactagaag tcttcacaca
     8101 ctaaataact taacaaactt tatcacacag ctaatcgcta ataatagtgt agacaaacgt
     8161 gatagttcat taaatacccg tcagaaactc ctgaaggttc taaagcaata cctgtcagtc
     8221 ttattttagc atagatgatg gtctttttat aggcggtcac taacatgaca tacaaaaagg
     8281 cagcctgtga aagaccgacc gccttttttt cagagacaaa atactttaac ttattgctat
     8341 cagcaactat caatctcgca atttttgcaa ttcttgtgcc aataattgtt gagcgacttg
     8401 tggattagct tgacctttgg ttgccttcat caagaagcct gtgaaagcct tatcagcatt
     8461 acgtttgcct gatttgaaat cagctactgc agcttcatta tcagcaaaga cttggtgaat
     8521 aattggtacc aaaacagcgg gatcagaaat ctgaacaagt cctgcctttt cgacgtaggc
     8581 acgtgctgag ccaccgtttt tagccaaatg cacaaagacc ttcttagcca ttttagaaga
     8641 gattgtacca tcagcaatga tagcaatcat ctccaccaaa ttttctggtg tcagagcgat
     8701 ttcttcaatg gttttgcctt ctgcatttaa gaattgggca acttctcctt gcaaccagtt
     8761 agagacttgc ttggcatcgc caccaagact tactgcagtt tcaaagaaat ctgacaagac
     8821 tttggtagct gttagctggc tagcatcata agctgagagg ccaagttcct cttcgtattt
     8881 agcacgacgc tgagctggga attgaggtaa ttgagcacgc atctcgtcaa tccaagcatc
     8941 atcaatctca tataatggca ggtctggttc tgggaaataa cgatagtccg ctgccccttc
     9001 tttgacccgc atcaaaatag tccccttatt cgcttcgtca tagcggcgcg tttcttggcg
     9061 tatcacacca cctgaacgca agagtttggc ttgacgttcc acctcaaatt caagcccttt
     9121 acggacattt gagaaggagt tcaagttttt caattcggtc tttgttccaa attgttcttg
     9181 cccgtaagga cgaagagaaa tattggcatc cacacgcatg gagccttctt ccatcttgac
     9241 atcagaaata cccgtatact ggatgatttc tttcagagct gtcaaataag cataggcttc
     9301 ttcaggagaa cgcatgtcgg cttctgacac aatctcaatc aaaggaactc cttggcggtt
     9361 aaggtccaca taagaataac catctgtgcc atgagtgttt ttaccagcat cttcttccaa
     9421 gtgtgcgcgt tcaatacgaa ttttcttggt tgaaccgtct tctagtttga tatcaatcca
     9481 gccgttgtag ccaatcggct catcaaactg tgaaatttga taagctttag gattatctgg
     9541 gtagaaataa tttttgcggt caaagtgcat ctctttatgg atgtccatat taagggcaag
     9601 cgctgctttt atgcccgcat caatcacccc tttgttcatg actggtaaga cccctgggaa
     9661 agaccaatca atcacattcg tgttggcatt tgggtcctca ccaaagtgag ctgacgaagg
     9721 tgagaaaatt ttggaattgg tatttaattc cacatggact tcaagcccaa taatggtttc
     9781 aaaattcatt acttgtcacc tccaaaaata atcggttgtt gcttgtgata atctgtcacc
     9841 gcttcaaagg ctgctgctgc ttgatagatg gtttcttcag cgtatttcgg accaattaat
     9901 tgaagcccta ctggcaagcc atcgacgaat cctgctggaa tagaaatacc tggcagacca
     9961 gccaagttaa ccggaatggt caataagtcg gccaaataca tggcaacagg atcgtggttg
    10021 agtgtgtcta agccaaaagc aaccgtcggt gttgttgggc ctagaataag atcataatcc
    10081 gcaaaaacct tgtcaaaatc ttgaataatc aaagtgcgca cttgaccggc ctttttgaaa
    10141 taagcgtcat aataacccga tgacaaacta aaggttccaa gcatgatacg acgtttcacc
    10201 tcatcaccaa agccttggct acgagtgtta acatagattt catccaagtt tttagcatcg
    10261 tcagcacgga aaccatagcg aatgccatca aaacgttgta agtttgaaga cgcttctgac
    10321 gaagcgataa tataataaac agccacaccg tacttgctat gtggaaggct gacttcctca
    10381 acagtagctc ctaaggcttc aaattgttta actgacgcaa gaaccgtttc cttaatctca
    10441 ggatcaatcc cttcgccaag atactcttta ggaagggcaa tcttcatgcc tttaatgtca
    10501 cgaccaatct tagatgtgta gtctgcaatg cggacaggcg ctgaagtggc atccttgaca
    10561 tcgctacttg caatcacatt taataattgg gcgttttctt taactgtcgg agcaaaaggt
    10621 ccaatctgat caagtgaact tccaaaagca atgagaccat agcgagaaac agtgccatag
    10681 gttggtttca aaccgacaac accattaaat gcagctggct gccgaataga accaccagtg
    10741 tctgaaccaa gagataaacg gacttgtcct gaagcgactg ctgttgccga accacctgat
    10801 gagcctccag gtacttttgt gtggtcccaa gcatttttcg ttttcttgaa ataagaggtt
    10861 tctgttgaac cacccatggc aaattcgtcc atgttggttt ttccaataac aatcatgtct
    10921 ttagcgtagg catttgctac tgatgtggca ttaaaaatcg gctcgtagtt atacaacatt
    10981 tttgaagcgg ctgtggttag gatttccttg gttgaaatat tatccttaac agctaacgga
    11041 atacctgaca tgaggttatc agcatcaata cctttggcat caattgccgc agcttgttta
    11101 agagcaactt cttctgaaac agtgataaac gatccgacag cctcctcacg agacttgatg
    11161 tcttcaagtg ttgcttgggt caattctgtt gctgaaatct ccttagctac aaggaggtca
    11221 tgcaactctt caatggtttt atgattaaaa gacattaggc atctcctccg tcatccaaaa
    11281 tagcaggtac cttgataaag tgattttctt tttcaggaac atttttaaag agcaaggcac
    11341 ggtctgttcc ttcttcagcg acatcttgac gcatcacatt tttcttatct gccatggttg
    11401 ttgtaatggc gacaccttcg gtatcaactt cgttcagcaa ttcaaccata tcaacgattt
    11461 tggataaggt cgttgcaaag gttgttgtct cactttctga aaatgataat tttgatagct
    11521 tcgcgacatg gcgaacttct tcttcagaaa ttttcatgat ttctcccatc caagatgatc
    11581 agaagtattc caattccaaa gaacagccct gctattctcc aaaggtagcg gaaaaactcc
    11641 tcacatcatt tttacatagt ctcaattatt atatcacaat tgctcccatt taccataggt
    11701 gactaatgca attctagaaa gccatacagc aaaaaacttc ctgactaagg tcagaaaacc
    11761 ttagtcagga agtgtgacaa actaaactct atttgtcttg ggtaagactg tggcctgaag
    11821 ctttgatggc agcctgagca gccgccaagc gagtaagcgg cactctaaat ggcgaacaag
    11881 aaacgtaggt caggccttgg ctgtggcaaa aggcaatgga agcaggatcg ccaccatgtt
    11941 ccccacaaat accaagtttc aactcaggct taaccgaacg ggctaaagag acggccaatc
    12001 cctttctgat caaaggtctg gaacggctcc ttatcaaaaa tacccctgtc aacgtattct
    12061 tccaagtatt ttcctacaaa catcacagaa aaagtaacgt tctttcaagt cggtaatatg
    12121 tttacgaagg tagtaaaggg gtgctttatt ctaatttacc aaatcctcaa cgattgtaac
    12181 aacatgtgat gtgataagcc taagataaaa atggtacaga ctctagcatt caagagtttg
    12241 aagctttatt tatattctaa tgctgaaaag actagagttt gtctaatggc tgtgagacga
    12301 tcctctaaac catcaactca aatcatttct caaacagcta aaatcctgtc tccttcaaaa
    12361 aaacatgtgt tctatctgac acagtccaaa gtttagttgc cttttagaca gcacaagaat
    12421 catcaggact aattctgacc agcatgtcac atacctcatc tacctaaccc atacatacgg
    12481 taaaaccaca tagagcaatg attacggtta ccaaactctt agtcactgaa tcaatagttt
    12541 ggtaggctct tttagtaatc gttaataagc ttagcaccaa gaacctgctc aaagtgagcc
    12601 aaagaccact cataagattc ttttgtaagg ctagcgacag ccgatttaac aatctctaat
    12661 tgataaccaa gatgataagc gtcaatagca gtatgtaaga cacagatatc agataaaact
    12721 cctgttaaca ctaattgagt gatacctctt tctcgtagtc taatatctaa atcagtcccc
    12781 gaaaaagctg aataataacg cttatcaatc caaaaaactc tgggatgttg cttcatataa
    12841 ctgtaaactt cagctagtgg accataaaga tggcgtccag tagttccttt gatattatgt
    12901 gctgcaaata gcttactctc aggatgccaa gaatcatttt gatcatgaca atcaatagca
    12961 aaaaaaatat agtctccttg atcaaatgcc gttttagtta cctctgctat tttagtggca
    13021 atagcctgag cagatttacc tgcacttaat ttaccatcat ctgctacaaa atcatttgtg
    13081 taatcaattg aaatcaatgc tctcatcact atctcctaaa cagggaaacc tctcccctta
    13141 tctcttatat atggcattgt atcagttttt attacaggta tcaagacatc tatttgcatc
    13201 taccattaac agcaaaaaac atctcttttc aagagatgtt gtctttttct atttaaaatt
    13261 cttttaattt agcaaaaata ccttcgttga taactttgag gtatgtccct ttcataccaa
    13321 gagaacgact ttcaataatc cctgcacttt ctagtttacg cagagcattg acaataacag
    13381 aacgggtaat accaatacga tcagcaataa cagaagctgt caatcgtcct tcgttaccat
    13441 ctaactctcc aagaattgct gcaacagctt tcatttctga ataagaaagc gtattaattg
    13501 ccatgttgac cgctgtttgt ttacggatgg tgtcttctaa gttttctgtc tgaagattta
    13561 atagttgaat ccccacaaca gtacttgaga tctcaaccaa aatcaaatca tcatcactaa
    13621 actcattgtc attacgccag ataatgagtg atccaaggcg catccctcca ccataaatcg
    13681 gcgcaatagt cgttagacct cctggataag tgtctttaga ctcaacaggg aatatagtca
    13741 attcattttc gacagaaagg ttagcttctg tatcgtaaac tcggctagca gcctttacat
    13801 aagtatctgg aaattgttta gcttcaaaaa attcctcaac acgatcagtg ttagttttat
    13861 atttcatggc ataaccaagc agtgtgccgc cgccattgat aatacaagca ttgcagtcaa
    13921 tgatatctgc taggcgagat gccattgtgt tatacggtaa ttctgtttct aggctatcta
    13981 cggaacgctg caaaatagat gtgattttac gagttttttc taataagtta ggcatttttt
    14041 tccttttcct ttgaattatt tgcgctttca ttataacaaa gctaactcgc ttttgcaagg
    14101 aattatcaga aaattttcta ttcttactac aattccaact attgatgcct tattatcact
    14161 tcatttaaag gagataagca ctagcttgtt agagcatagg gtcacattct tttaagaaac
    14221 gctattttta ttaatcaaag atgagcattg cattaacgtc tatactggta taagacacgt
    14281 gtaatctttt cttggacatc ttctaattct tctacacttg gtaggtaaac aatacggaag
    14341 tgatctggat ctttccagtt aaaaccacgt ccatgtacca acatcacttt ttcttgcttt
    14401 aataactgta agacaaattc ttcatcatca tcaatacgat acatctgacg atcaattttc
    14461 ggaaataaat aaagacctgc ttcaggttta actgccgata agccagggat agcattaata
    14521 gcttcataaa taaaattacg ttgctcaaaa atacgccctc ctggcagtaa cagtgcatca
    14581 actgattgat gacctcctaa ggaagtttga acaacttgtt gggctaaaac attggagcat
    14641 aaccgcatat tggcaagcat gtttaaccct tcaatgtaac ctttaacatg gtctttagga
    14701 cctgataaga ccatccatcc aacacgaaaa ccagcaatac gatgagattt ggaaagacca
    14761 ttcatagata cacaaaaaac atcaggtgcc aaacttgcaa tagcaatatg ctctttacca
    14821 tccatcacca aacgatcata gatttcatcg gcaaaaataa tcagctggtg ttctcttgcc
    14881 aaagcaacga tgtcttctaa aatctcctta ggataaaggg ctcctgttgg gttattagga
    14941 ttgatgacca caatagcttt ggtgcgactc gtgatttttg atttgatatc agcaatatca
    15001 ggataccaac cagcttcttc atcacaaaga taatggaccg cttttcctcc accaagactc
    15061 acacaagccg tccataaagg ataatccggc attggtacta aaacttcatc gccattatct
    15121 agcaaagctt gaagggatat ggatatcaac tctgacacgc catttcccaa ataaatatct
    15181 tcaatgtcaa catctgggaa gcccttgagt tggcaatact gcatgatagc cttacgcgct
    15241 gaaaaaatcc ctttactgtc agaatagccc tcacttagcc tcgcgttgac aattaaatca
    15301 cgaatcactt catctggtgc ttcaaaacca aaagcagcag gattgccagt atttaaacgc
    15361 agaatttttt caccactcgc aatcatgcga ttagcttcat ccaaaacagg ccctcgaata
    15421 tcataggcaa catgttctaa ttttgatgat ttttcaataa ttttcatagt ctcatacacc
    15481 tcgtattcca tattataaac caaaatactt aaaatagaaa gaatttatcc atcaaagctt
    15541 aggacagcta atcgttttaa cacaggtctt atgagacgat aatacagtct ttagtttagc
    15601 aaaaataatg gatcgctttc aaaatgactc aaaaaagagt ataatgaaaa aaagaataaa
    15661 agctattctt gtcaaactgt taaaggagga cagttctatg tcactaaaat ataaacgtat
    15721 cctcgttgct attgatggct cttatgagtc tgaattagct ttcaataaag gcgttaacgt
    15781 agcactacgt aacgacgcta ccttactact agtacacgtt attgataccc gtgccttgca
    15841 aagcgtagct acttttgata cttacatcta tgaaaaatta gaacaagaag ctaaggatgt
    15901 tttagataat tttgaaaaac aagcacagat agcaggaatt acaaacatca aacaaattat
    15961 tgaatttggc aaccccaaaa acttactagc tcacgatatt ccagatcgtg agaacgctga
    16021 tttaattatg gtaggagcta ctggtcttaa tacctttgaa cgcctcctta ttggctcatc
    16081 ttctgagtac attatgcgac acgctaagat tgacttactt gtagtccgag atagcactaa
    16141 aacactctag tctattacaa ttttcaaaca ccaaaaaaca agcagcatct acaacgaaat
    16201 tgcttgtttt tttgtgctga gctaagtcta gtcaatatct ttttatttct tgatatttaa
    16261 aagagtaaac ctctatttat cgaatcgttg tagtgatttt tgtaattgtt tgtctaactc
    16321 tttttttatg gctacttcag gtgcgtgacg ctcttgccaa tagtcaggtt tttgaatttt
    16381 atgggtaacg gggtcaaagt gtgccttgcc atctggaaaa attttagcca tatttgcttc
    16441 atgtactgct tcaaatatag gttgtggatc tactcccatc agtacaaaag aaccataagt
    16501 aaaataaagc aaatccgcta aggcatcaac ttgcccaatc aggggcgttt caacatgttt
    16561 tttagcttgt accttattag ctgcttggtc aacagcacca tgcaaatcga aaatagcctg
    16621 tgtaaattgt tgttggtctc ccttgcttgc tgcgtaaaga aactcaacaa tttcttctac
    16681 tttaaagcct gacctataac cagcttctga aatggtataa ctacgcggtg tttcaatagt
    16741 atctgagtcc atcagacgat gaaaatcttt gactttatta aaattttcat cacggctttt
    16801 aaagctcttc tctatatcaa aatggatgag accgtagtga gctaaggctt tggaaatccc
    16861 atcattatta ttagagtcgg tagtgaaatg ggctccatct tttaccacag tttcagcatt
    16921 tcccatagca ataccgatac caactcctga taacatttca aggtcattat ctgaatctcc
    16981 aaaagccatc acttcagaca agtcaaatcc aaacatctct cccaaacgtt caattccttt
    17041 tatttttgat tgatcaacag agatcagatc caaggaataa ggactacttc ttgtaatttt
    17101 tatatgaggg aacttctctt gtattttctt agtttcagct tgtgacgcta ccagaacaac
    17161 ctgataaata ggctcccgca taatagttac taaatttcta aaactctgag gcttgattcg
    17221 gcgaatcaaa tgcttaaaac taccctcaac tgttcttgcc caacttttgg gcacaaaact
    17281 actaatgact tgtccaaatg ggctagtccc catatcaata atacgcgatc ctgctaatcc
    17341 agaagctgtt ccaagagaaa tttctcgctt tttttcgttg gcatagcgaa tcactttgta
    17401 aatcatcgac tttggcaatt gattctgata taagacctta tctcttgtta agatatattg
    17461 cccattgtaa gtaacggcaa aatctaatcc aaaattttct agaaaaggtt gtacaaatcc
    17521 cggtccacga ccagttgcta aaccgaccat aatgccttgt tttttgagtt gttggatggc
    17581 tttttgcgtt gttttttgga tatttttacg atcattgaga agggtcccat caatatcaaa
    17641 aaagactgct tttactgtca aaaccttctt ttgcctcctg tgtttatgat agaatagatt
    17701 atatcataaa taaaaggaag tttgacaatc aaatgaaaaa aatcctagtc ttacacactg
    17761 gtggaaccat ttctatgcaa gctgataact ctggtagggt tgttccaaat caagataatc
    17821 ctatgacgaa aatacatgct gcagcccaag atattcagct taccgttagt gattttctaa
    17881 atcttccgag tcctcacatc acgcctcacc acatgttatc tatttatcat catattcaag
    17941 aaagaactga tgtctttgat ggtatagtca tcacacatgg aacagatacc ttagaagaaa
    18001 cagcttattt tttagatacc atggcattgc ctactaatat acctgttgtc ctaacaggtg
    18061 ctatgaggtc ttctaatgaa gttggtagtg atggcatata taattacctt accgcactta
    18121 gagttgcaag tagtgataaa gctaaagaaa aaggggtact tgttgttatg aacgatgaaa
    18181 tccatgctgc taaatatgtt actaaaacac atacaaccaa tatttcaact ttccaaacac
    18241 ccacacacgg accgcttggt atcatcatga aaaatgacct actttttttc aaaactgctg
    18301 aacctcgtat tcgttttgac ttgagatgca tttctggaac catccccatt atcaaagcat
    18361 acgcaggtat gggcgatggt agtatcttga gtctattaac tccaggtagc attcaaggtc
    18421 tagttatcga agctctaggg gcaggaaatg tcccaccatt agcagttggg gagatcgagc
    18481 acttaattac tttaggtatt cctgtcattc ttgtttcccg ttgtttcaat ggtatggcag
    18541 aacctgtcta tgcttatgaa ggtggtggtg cgatgctaca agaagcaggt gttatgtttg
    18601 taaaagaatt gaatgctcca aaagcccgtc taaagttatt aattgctctc aacgccggac
    18661 ttactggtca agagctaaaa gattatattg aaggataatc tagcttcctt tagaaatgtc
    18721 ccactaaaac atattagtgg gacatttcta gtactttaag ctttaaatta ggacatctcc
    18781 taaatgtcta gaataaacaa ctatgtttca aactatgaga ttgtgcactc ataatcattt
    18841 ctataacagt ttgagaccag tattccaata aatccaactc ttagcaatca gataccgggt
    18901 aaacaagtct ttattttgtc tcattaagta tgagcttact tgaataataa caaaactact
    18961 tgcttatcct tttttagtaa tgatgaagac gacctttttc taagaacttc tttgtctttt
    19021 aaaataaaca aagcacatat taccctttat tttagttctc gtttggaaaa aagaaagaca
    19081 ttgcctaaaa gataacaaag agttaaacca aaggccacta atatgctagt actagaaagg
    19141 tgtttatcac caagagacaa ggataggtgt ttaaacatca aaactgggct aatatctaac
    19201 ttaaaaaaat ggtttgctaa agtagcagat actttatcaa aaagaaggac ataaagtata
    19261 taagctgtga ttgactgata agttttacga agtatattaa acaaaaattg acacaaagca
    19321 ataatagcaa tataaaaagg cagttgttta atcatccgat ctatgactat tccatagtat
    19381 gcttgaccag taaataaatg aaatgctaaa gcactaagaa gataagttaa actaataaat
    19441 aaaagagata agccaaaact aactagcagt ttatagatta taattaaagt acgattttgt
    19501 tgtttgacca caacattatt aatcgtacgg tagttatagt catcactaaa aaagatacgg
    19561 gctggtgtta aaaagaaaag cgtcacaaaa tcagtaataa tattaatgat ttgtatcatg
    19621 atgacttggt taggtgtctt gcgaaaacta taggctacac caacagcaat gacaacaagt
    19681 agcatacaag aaaatagagc taaacgctct tggcgtatcc gaaataaatc tgctttaaga
    19741 taggaaatca taaaaacctc tttctaaatt aatggtgatg ctgcatcttt acctaacaaa
    19801 tcgaggtaat aatgttcaaa gctcacttga tgatgataaa tgtcaaaaat ctcaacaggc
    19861 agatctagaa caattttgat aagctccatt actgataaag ttggttttat tagtagcatc
    19921 tgaccatcag tctcaactct aagccccaat tctaaaagtc tattttttac cagctgatca
    19981 tcgcttgtat gaagggcaat ttgtactttt acttgagctt taagctctgc tttgtccata
    20041 ctcttaataa ctttgccttt atgcataatc acgtaacgat caacaactaa gtctaattct
    20101 gataaaatat gactggaaat caaaatcgta ataccatagc aatctcttag gttcaaaata
    20161 atctgacgca tttctttgat tcctgctgga tcaagaccat taatgggttc atccaagatt
    20221 aaaaattctg gaaaatccag tatcgaaaga ccaatagcca aacgttggcg catccctaaa
    20281 gaacaatctt tcaccttttt ctttttgctc actttttcta acccaataag ttccaaaacc
    20341 tcatgtatgc gctcttttgc atcagctaat ctcaattgca acgctgcata gtaaagatta
    20401 tcatgagctg tcatattggg ataaagtgtc ggtgactcaa taattgatcc cattcgatag
    20461 ggttgtaact gtttgatttg ccctgaatct tttccaatta ggccagataa aatacgcatt
    20521 aacgttgtct ttccagcacc attttgaccc acaaggccgc aaatttctcc tttgtaaata
    20581 tcaaaagaga tatcacttaa aacctgattt ttaccatagg atttatttac ttgctcaata
    20641 cttagtaaca ttgattgctg tgtcattttc cgctcctttt ttgtacttat cgatcaatac
    20701 aatatcttac acataataac atctgtaaac gg
//
LOCUS       DAXWIQ010000033        20585 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000033 DAXWIQ010000000
VERSION     DAXWIQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20585
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.033"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            554..943
                     /locus_tag="ACGEUI_001239"
     CDS             554..943
                     /locus_tag="ACGEUI_001239"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFF8166077.1"
                     /translation="MGTTDLVSCAVQASEYAYVPYSHFPVGAALKTKDGTIYTGCNIE
                     NVSFGLTNCGERTAIFKAISDGHKELVEIAIYGETMQPVSPCGACRQVMAEFFDPSSL
                     VTLIAKNGQTVEMTVGDLLPYSFTDLI"
     gene            1037..2089
                     /locus_tag="ACGEUI_001240"
     CDS             1037..2089
                     /locus_tag="ACGEUI_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8166078.1"
                     /translation="MNKKFIGLGLASVAVLSLAACGNRGASKGGASGKTDLKVAMVTD
                     TGGVDDKSFNQSAWEGLQSWGKEMGLQKGTGFDYFQSTSESEYATNLDTAVSGGYQLI
                     YGIGFALKDAIAKAAGDNEGVKFVIIDDIIEGKDNVASVTFADHEAAYLAGIAAAKTT
                     KTKTVGFVGGMEGTVITRFEKGFEAGVKSVDDTIQVKVDYAGSFGDAAKGKTIAAAQY
                     AAGADVIYQAAGGTGAGVFNEAKAINEKRSEADKVWVIGVDRDQKDEGKYTSKDGKEA
                     NFVLASSIKEVGKAVQLINKQVADKKFPGGKTTVYGLKDGGVEIATTNVSKEAVKAIK
                     EAKAKIKSGDIKVPEK"
     gene            2228..3760
                     /locus_tag="ACGEUI_001241"
     CDS             2228..3760
                     /locus_tag="ACGEUI_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8166079.1"
                     /translation="MTQHVIEMREITKKFGDFVANDHINLNVRKGEIHALLGENGAGK
                     STLMNMLAGLLEPTSGEIVINDKPVQIDSPSKSAKLGIGMVHQHFMLVEAFTVAENII
                     LGNEVVKNGCLDLNQASKDIKVLSEKYGLAINPSAKVSDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPAEIKELMTIMKNLVKEGKSIILITHKLDEIRAVADRVTVIRRGK
                     SIETVDVAGATSQDLAEMMVGRSVSFTTSKKAAEPKDVVLSIKNLEVDENRGVPAVKG
                     LSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVKSGSIMIKNNEVTHLSSRKITELS
                     VGHVPEDRHRDGLILDLSLAENTALQTYYKQPLSQNGILNYTKINDYARQLMKEFDVR
                     GANELVPARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIKERD
                     KGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVSPENTNKQELGILMAGGSIHKEEG
                     HV"
     gene            3753..4817
                     /locus_tag="ACGEUI_001242"
     CDS             3753..4817
                     /locus_tag="ACGEUI_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8166080.1"
                     /translation="MSKNAQKIAVPLISVLLGFLLGAIIMVIFGYDPIWGYEGLFQIA
                     FGSVKNIGEIFRSMGPLILIALGFTVASRAGFFNVGLSGQALAGWISAGWFALLNPDM
                     PRPLLILMTALIGMIAGGIAGAIPGILRAYLGTSEVIVTIMMNYIILYVGNAIVQRGY
                     PESVKQSIDSTIQVSDNASYQTHWLSALTNNSRINIGIFFAIIAIALIWFLLNKTTLG
                     FEIRSVGLNPHASEYAGMSSKRTIILSMIISGALAGLGGVVEGLGTFENVFVQGSSLA
                     VGFDGMAVSLLAANSPLGIFFSSFLFGVLNIGAPGMNIAGIPPELVKVVTASIIFFVG
                     SHYLIERYIIRPKKLVKGGK"
     gene            4819..5775
                     /locus_tag="ACGEUI_001243"
     CDS             4819..5775
                     /locus_tag="ACGEUI_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002268043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8166081.1"
                     /translation="MSLVTIFALLMSSMLIYATPLIFTSIGGTFSERSGVVNVGLEGI
                     MVMGAFSGIVFNLEFAETFGKATPWIAVLVGGIVGLIFSLIHAVATINFRADHIVSGT
                     VLNLLAPSFAVFLVKAMYGKGQTDNIQQSFGKFDFPGLSQIPVIGDIFFKNTSLIGYF
                     AIAFSFFAWFLLYKTRFGLRLRSVGEHPQAADTLGINVYLMKYYGVMISGFLGGIGGA
                     VYAQSISVNFAVTTILGPGFIALAAMIFGKWNPVGAMLSSLFFGLSQSLAVIGAQLPL
                     LEKIPTVYLQIAPYMVTIIILAAFFGQAVAPKADGINYIKSK"
     gene            complement(5988..7706)
                     /locus_tag="ACGEUI_001244"
     CDS             complement(5988..7706)
                     /locus_tag="ACGEUI_001244"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFF8166082.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDENKSSGLIQVIGEDVDMEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVEADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQTATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATIGDTLDIAQEKIANIETE
                     INTFVG"
     gene            complement(7882..8451)
                     /locus_tag="ACGEUI_001245"
     CDS             complement(7882..8451)
                     /locus_tag="ACGEUI_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8166083.1"
                     /translation="MNHRKSADISRIAIFFAIMLVIHFVSSLVFNIWPIPIKPTLVHI
                     PVIIASVLYGPRIGAILGGLMGIISVITNTIILLPTNYLFSPFVDHGTFASLIIAIIP
                     RILIGITPYYCYKLIPNQFGLIVSGIIGSLTNTIFVLSGIFIFFATVFDGNIKALLTA
                     IISSNAIVEMIISAIITFVLIPTLSRLKR"
     gene            complement(8509..9054)
                     /gene="coaC"
                     /locus_tag="ACGEUI_001246"
     CDS             complement(8509..9054)
                     /gene="coaC"
                     /locus_tag="ACGEUI_001246"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFF8166084.1"
                     /translation="MTKHITLAVSGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNAIHLDVMDEHDPKVINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     LVTSVALALPATTPKLIAPAMNTKMYQNPITQENIKRLSTIGFTEIPPKSSLLACGDK
                     GPGALADIDVILATIDTIWKL"
     gene            complement(9047..9733)
                     /gene="coaB"
                     /locus_tag="ACGEUI_001247"
     CDS             complement(9047..9733)
                     /gene="coaB"
                     /locus_tag="ACGEUI_001247"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFF8166085.1"
                     /translation="MKLIITSGGTTEPIDAVRGITNHSTGQLGKLITERFLQYHHDVT
                     LVTTKTATKPLPNKRLRIIEVETVNDLMAALKDQVPHHDILIHSMAVSDYTPVYMTDL
                     EQVSQADNLNCFLCEHNSEPKISSASDYQVLFLKKTPKVISYVKQWNPNIKLVGFKLL
                     VNVPQEELIKVARASLAKNHADYILANDLVDIQTGMHKALLISNNEVASADTKEAIAD
                     LLYERMTKHD"
     gene            9935..10771
                     /locus_tag="ACGEUI_001248"
     CDS             9935..10771
                     /locus_tag="ACGEUI_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HFF8166086.1"
                     /translation="MITIRDLASLPIFVYGDGNKKVPGALSPFVWTEVFLKTINQEPN
                     QLILHIWPMTRTVILGMLDRQLPYFELAKTEIGNNGYVPVTRNIGGLAVVADDGILNF
                     SLVIPDHFSESISISNAYLIMVDVIRESFSDYYQRIEYHEIKNSYCPGNFDLSIAGRK
                     FAGIAQRRIKKGIVVSIYLSVCGDQAARGQLIKDFYEAGTQGEVTKVNYPQIDPECMA
                     TLSELLETPFTVAEVLERLRLTLRQLGFSLTEKSPDQALLTNFDAVYERMQLEVVRKE
                     GK"
     gene            10768..11967
                     /locus_tag="ACGEUI_001249"
     CDS             10768..11967
                     /locus_tag="ACGEUI_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFF8166087.1"
                     /translation="MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDD
                     VSYALRRAASAPLQITGAAYVDPYGQLFEYGFSVTKDADISGLKELAQAMKAKGAKVV
                     LQLTHAGRFASHALTKYGFVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRA
                     IQAGFDGVEVSSAQRLLIQTFFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVIKQ
                     EAPDGFIFGFRATPEETRGNDIGYSIDEFLQLMDWVLNVAKLDYLAIASWGRHVFRNT
                     VRSPGPYYGRRVNQVVRDYLRNKLPVMATGGMNTPDKAIEALAHADFIGVSTPFVVDP
                     EFAHKIKEGCEESIHLRIRPADLKSLAIPQASFKDIVPLMDYGESLPKESRTLFRSLT
                     HNYKEIK"
     gene            11964..12962
                     /locus_tag="ACGEUI_001250"
     CDS             11964..12962
                     /locus_tag="ACGEUI_001250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFF8166088.1"
                     /translation="MKVSILDYGVIDKEKTPQEALLETRCLAQVADKLGFHRFWVAEH
                     HNIYAFAISSPELLMMHLADHTKQIRIGSGGIMPLHYSSFKIAEWIMTLEALHPNRID
                     LGIGNSLGTTLVQRALSSIHCKDSYSQVVTELYQYLNPDHLSPLPIFVNPRGNTYPQI
                     WTLSNSLETAELAGQLGLGYTFGIFPYIPKDPITEAKRVSAHYRKAFRPSKLLKIPKL
                     ILAVFIVLSDTDEKAEALAKPLDIWMLGQQDFNEFKTYPDVEEARNYHLTEKQREAIA
                     ANRSRMVIGSPHTVKKQLDRLIEACQADELLAIPLVPEFANRQRTLELLADLYVTI"
     gene            13004..13336
                     /locus_tag="ACGEUI_001251"
     CDS             13004..13336
                     /locus_tag="ACGEUI_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFF8166089.1"
                     /translation="MKKIANYLLIEKTDDRYTISMTPELQDDIGTIGYAEFTDNDHLA
                     VDDIILNLEASKTVMSVLSPLAGAVVERNEAATLTPTLLNSEKAEENWIVVLTDVDQA
                     AFDALEDA"
     gene            13329..14141
                     /locus_tag="ACGEUI_001252"
     CDS             13329..14141
                     /locus_tag="ACGEUI_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HFF8166090.1"
                     /translation="MPSSFDLLGEMIGLLQTEQLTSSWACPLPNALTKRQDLWRALIN
                     QRPALPLSKDYLNLEDAYLDDWRASFVPVSVKDCQKTNYTSLFLYHGDIRYLAVDAIV
                     NAANSELLGCFSPNHGCIDNAIHTFAGSRLRLACQAIMTEQGRKEAIGQAKLTSAYHL
                     PASYIIHTVGPRITKGHHVSPIRADLLARCYRSSLDLAVKAGLTSLAFCSISTGEFGF
                     PKKEAAQIAIKTVLKWQAEHPESKTLTTIFNTFTSEDKALYDTYLQKENNCE"
     gene            14134..15015
                     /locus_tag="ACGEUI_001253"
     CDS             14134..15015
                     /locus_tag="ACGEUI_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HFF8166091.1"
                     /translation="MSNWTTYPQKNLTQAEQLAQLIKEADALVVGIGAGMSAADGFTY
                     IGSRFETAFPDFIAKYQFLDMLQASLFDFEDWQEYWAFQSRFVALNYLDQPVGQSYLD
                     LKEILGTKDYHIITTNADNAFWVAGYDPHNIFHIQGEYGLWQCSQHCHQQTYKDDTVI
                     RQMIAEQKNMKVPGQLIPHCPECEAPFEINKRNEEKGMVEDADFHAQKARYEAFLSEH
                     KEGKVLYLEIGVGHTTPQFIKHPFWKRVSENPNALFVTLNHKHYRIPLSIRRQSLELT
                     EHIAQLISATKTIYQKS"
     gene            15062..16081
                     /locus_tag="ACGEUI_001254"
     CDS             15062..16081
                     /locus_tag="ACGEUI_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFF8166092.1"
                     /translation="MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDP
                     KVEIGKFQNAVVETNQEYLKEHHIPVVRRDTGGGAVYVDSGAVNICYLINDNGIFGDF
                     KRTYQPAIEALHHLGATEVEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDF
                     EAMEKALKPNRKKIESKGIRSVRSRVGNIREHLAPQYQGITIEEFKDLMVCQLLQIET
                     ISQAKRYDLTEKDWQQIDALTERKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIK
                     KGYIAACRIYGDFFGKADIAELEGHLIGTRMEKEDVLATLNAIDLAPYLGAITAEELG
                     DLIFS"
     gene            16209..17879
                     /locus_tag="ACGEUI_001255"
     CDS             16209..17879
                     /locus_tag="ACGEUI_001255"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFF8166093.1"
                     /translation="MKSDIEIAQSVALQPITDIVKKVGIDGDDIELYGKYKAKLSFEK
                     MKAVEANEPGKLILVTAINPTPAGEGKSTMSIGLADALNQMGKKTMLALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLQQGNDLGIDPRR
                     IIWKRVLDLNDRALRQVIVGLGSPVNGVPREDGFDITVASEIMAILCLATDLKDLKKR
                     LADIVVAYTYDRKPVYVRDLKVEGALTLILKDAIKPNLVQTIYGTPALIHGGPFANIA
                     HGCNSVLATSTALRLADYTVTEAGFGADLGAEKFLNIKVPNLPKAPDAIVIVATLRAL
                     KMHGGVAKSDLAAENCEAVRLGFANLKRHVENMRQFKVPVVVAINEFVADTEAEIATL
                     KALCEEIKVPVELASVWANGAEGGLALAKTVVRVIDQEAADYKRLYSDEDTLEEKVIN
                     IVTQIYGGKAVQFGPKAKTQLKQFAEFGWDKLPVCMAKTQYSFSDNPSLLGAPTDFDI
                     TIREFVPKTGAGFIVGLTGDVMTMPGLPKVPAAMAMDVAENGTALGLF"
     gene            17964..19541
                     /gene="cls"
                     /locus_tag="ACGEUI_001256"
     CDS             17964..19541
                     /gene="cls"
                     /locus_tag="ACGEUI_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFF8166094.1"
                     /translation="MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQ
                     SYAWMEQYRVWITILESVFAITIVLYLVNSDMDAISRMTWLILIMIAPLLGSLFLIYT
                     KLDWGYRGLKQRINHLVDLSAPYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIY
                     NNTRVTYFPTGETFFDSLKEQLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVE
                     VRVLFDGMNELSTLSSDYAKRLEQIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEV
                     SFTGGINLADEYINEVERFGHWKDAGLMLEGEATDSFLILFLQMWSITEKELIIDPYL
                     SDHSLKLPSDGYVIPYGDSPLDTDKIGKNVYIDILNHAKEYVYIMTPYLILDSEMEHA
                     LRFASERGVDIRIIMPGVPDKGVPYALAKTYYKALMSSGVKIYEYQPGFVHSKVFISD
                     NTKAVVGTINLDYRSLYHHFECATYLYRVSVIADIVNDFNEAQKQSLLMTSDHLTQRP
                     WYQKLIGLLVRTIAPLL"
     gene            19881..20219
                     /locus_tag="ACGEUI_001257"
     CDS             19881..20219
                     /locus_tag="ACGEUI_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HFF8166095.1"
                     /translation="MQIIKRDGHVADFDPDKIYQAIIKAARTVYVIDDTWRQNLTHVT
                     KKVVIDLEEAHVERPTINMVQSLVENRLMDAGYITIAEHYISYRLQRDLERNGYGDKI
                     VVHLRFEQIK"
BASE COUNT         6308 a         3482 c         4257 g         6538 t
ORIGIN      
        1 tctactcgag taaccttttt taatgtattt taactgaaaa aggctttcat aattttaaaa
       61 aaatgttaaa ataggtgtat aacaactgat taccaagaga ttattatcat tatcttctaa
      121 ggtgtcattg ttatggtgac aatcaaccat acgctactaa agataggtag cgttcgatca
      181 caggttgatc aagtgattga aaagtcaata tttataattt tacaaatatc tgtatcaatc
      241 catgttgaat agtctatgct tctaaggtgt cgacgttaat gtactgacgg gaccgttata
      301 gtctttcttt ttggagaaac gtcggatgcc agtgaactag ttgaagttaa agatgttaat
      361 tcaaacagtg ctgaagagat tgatatcgtt ttgaacggtc gtttacttca aagcaaagaa
      421 attaccagtc tatcggagat gatataagtg ctaaagttag atcacgttaa ttatctatca
      481 agtcgggtgc atatttcgtt aaagtatcta caggggtttc aatagatgta gctattatga
      541 aaggtgatgt taaatgggga ctactgattt agttagttgt gctgtacaag ctagtgagta
      601 tgcttatgtc ccttactctc attttccagt aggagcagct ttaaaaacaa aagatggaac
      661 gatctatact ggctgtaata ttgaaaatgt tagttttggt ctgacaaatt gtggagagcg
      721 aacagctata tttaaagcca tatctgatgg tcataaagaa ttggttgaaa ttgctattta
      781 tggagaaact atgcaacctg tatctccttg tggggcttgt cgtcaggtaa tggcagaatt
      841 ttttgatcct tcttctttag tcactttaat tgctaaaaat ggtcaaactg ttgaaatgac
      901 tgttggagat ttactcccct attcttttac agatttaata taatctgctt taatttctgt
      961 tatctagccg tcttggcttt atatttactt tcagcaactt atttgctaga aatctttttt
     1021 aggagggttc atattcatga acaagaaatt tattggtctt ggtttagcgt cagtggctgt
     1081 gctgagttta gctgcttgtg gtaatcgtgg tgcttctaaa ggtggggcat caggaaaaac
     1141 tgatttaaaa gttgcaatgg ttaccgatac tggtggtgta gatgacaaat cattcaacca
     1201 atcagcatgg gaaggcctgc aatcttgggg taaagaaatg ggccttcaaa aaggaacagg
     1261 tttcgattat tttcaatcta caagtgaatc tgagtatgca actaatctcg atacagcagt
     1321 ttcaggaggg tatcaactga tttatggtat cggctttgca ttgaaagatg ctattgctaa
     1381 agcagctgga gataatgaag gagttaagtt tgttattatc gatgatatta tcgaaggaaa
     1441 agataatgta gccagtgtta cctttgccga ccatgaagct gcttatcttg caggaattgc
     1501 agctgcaaaa acaacaaaaa caaaaacagt tggtttcgtg ggcggtatgg aaggaactgt
     1561 cataactcga tttgaaaaag gttttgaagc aggagttaag tctgttgacg atacaatcca
     1621 agttaaagtt gattatgctg gatcatttgg tgacgctgca aaaggaaaaa caatcgcagc
     1681 agctcagtat gcagcaggtg ctgatgttat ttaccaggca gcaggaggca ctggagcagg
     1741 tgtatttaat gaagcaaaag ctattaatga aaaacgtagt gaagctgata aagtttgggt
     1801 tattggtgtt gaccgtgatc aaaaagacga aggaaaatac acttctaaag atggcaaaga
     1861 agcaaacttt gtacttgcat catcaatcaa agaagtcggt aaagctgttc agttaatcaa
     1921 caagcaagta gcagataaaa aattccctgg aggaaaaaca actgtctatg gtctaaaaga
     1981 tggcggtgtt gaaatcgcaa ctacaaatgt ttcaaaagaa gctgttaaag ctattaaaga
     2041 agcgaaagca aaaattaaat ctggtgacat taaagttcct gaaaaatagt gtttaagaac
     2101 aagaagcgcg acctttgtcg gtcgctcttt ttaaactgag ataattttta tctcagtaaa
     2161 gtttgtgata ggtgatagat cataggcttt actgaaataa aagattgcat tgaaaggaaa
     2221 tgatcctatg acacagcatg tcattgaaat gagagaaatc acgaaaaaat ttggtgattt
     2281 tgttgcaaat gaccacatta acttaaatgt cagaaaaggt gagattcatg ccttattggg
     2341 tgaaaatggg gcaggtaaat caactttgat gaatatgcta gctggtctct tagagcctac
     2401 tagtggagaa attgtcatta atgacaagcc agttcaaatt gattcgccat ctaaatcagc
     2461 taagcttggt atcggaatgg tacatcaaca tttcatgtta gtagaagcct tcacggttgc
     2521 tgagaatatt attttaggaa atgaagtagt aaaaaatggg tgcttagatc ttaaccaagc
     2581 atcgaaagat attaaggtac tatccgaaaa atatggcctt gcaatcaatc catcagctaa
     2641 agtttctgat atttcagtag gagcacaaca acgtgttgag attttgaaaa ccttgtatcg
     2701 tggtgcagat attcttatct ttgatgagcc aactgccgtt ttaacacctg cagaaattaa
     2761 agaattgatg actatcatga agaatctcgt gaaagaagga aaatcaatta ttttaattac
     2821 ccataaactg gacgagattc gtgctgtcgc cgaccgtgtc acagttattc gtcgtggtaa
     2881 gagtatcgaa acagttgatg tggcaggagc aacttcacaa gatttagctg agatgatggt
     2941 gggacgctcg gtttccttta caactagcaa aaaagctgct gaaccaaaag atgtggtctt
     3001 atccatcaaa aatcttgaag tagatgaaaa tagaggggtt ccagcagtta aggggctatc
     3061 tctagatgtc cgagctggtg aaattgtagg gattgctgga attgacggaa atggtcaatc
     3121 tgagttaatt caagctatta caggtttacg taaagtcaaa tctggaagta ttatgattaa
     3181 aaacaatgaa gtcactcatt tgtcttcacg caaaattact gagttatcag tgggtcacgt
     3241 gccagaagat cgtcatcgag atggtttgat actagatctt tctttagctg aaaatacagc
     3301 cttacaaacc tattacaaac aacctctaag tcaaaatgga atcttaaatt atactaaaat
     3361 taatgactat gctcgtcaat taatgaaaga atttgatgtt cgcggagcca atgagctagt
     3421 accagctaga ggattttctg gtggtaacca acaaaaggcc attattgctc gtgaagtgga
     3481 ccgtgatcca gatttactta ttgttagcca accaacacgt ggtcttgatg taggagctat
     3541 tgaatatatc cataagcgtt tgattaaaga acgtgataaa ggtaaagctg tcctcgttgt
     3601 tagttttgaa cttgatgaaa tattgaattt atctgatcga attgctgtta ttcatgatgg
     3661 taaaattcaa ggtatcgtat cgccagaaaa taccaataaa caagaattgg gtattttaat
     3721 ggcaggtggt tcaattcata aggaggaagg tcatgtctaa aaatgcgcaa aaaattgctg
     3781 taccattgat ttctgtcttg ctaggatttt tacttggcgc tattattatg gttatctttg
     3841 gttatgatcc tatctggggg tatgaaggtt tattccaaat tgcctttggt agtgttaaaa
     3901 atattggaga aatattccgt tcaatggggc cattgatttt aatagcgctt ggctttactg
     3961 ttgctagccg tgcaggcttc tttaacgttg gtttgtctgg acaggcttta gctggatgga
     4021 tttcagcagg ttggtttgca ctcttaaatc cagatatgcc tcgtccatta ttgattctta
     4081 tgacagctct tatcggaatg attgctggtg gtattgctgg agctattcca gggatacttc
     4141 gtgcttatct tggtacaagt gaagttatcg ttacgattat gatgaactac atcattttat
     4201 atgtcggaaa tgctattgtt caacgtggtt atcctgagag tgtcaaacaa agtattgatt
     4261 caactattca agttagtgat aatgctagtt atcaaacgca ttggttatca gccctaacta
     4321 ataactctcg tattaatatt ggtattttct ttgccattat tgccattgcc ctaatctggt
     4381 tcttgcttaa caaaactact ttaggttttg agattcgttc agttggtctc aacccacatg
     4441 cttcagaata cgcagggatg tcatcaaaac gtactatcat tttatcaatg attatctctg
     4501 gagctcttgc aggtcttgga ggagtagttg aaggtctagg aacctttgaa aatgtttttg
     4561 tacaaggatc ttctttagca gttggttttg acggtatggc agttagctta ttagcagcaa
     4621 attcaccgct tggaatattt ttctcatcat tcctatttgg tgtcttaaat attggtgctc
     4681 caggaatgaa tattgcaggc attccaccag aacttgttaa agtagtaact gcatcaatca
     4741 ttttctttgt tggttcacat tacctcattg aacgttacat tattcgccct aaaaaattag
     4801 tgaaaggtgg taaataagat gagtttagta acaatttttg ccttattgat gtcatctatg
     4861 cttatttatg caacaccact cattttcaca agtattggag gaactttctc agagcgctca
     4921 ggcgttgtta atgttggttt agaaggtatc atggtcatgg gagcgttctc tgggattgtt
     4981 ttcaacttag agtttgctga aacatttgga aaagcaacgc cttggatagc cgttttagtc
     5041 ggtggtattg ttggtttgat tttttctttg attcacgctg tagcaaccat caactttcga
     5101 gctgaccaca ttgtcagtgg tacagtgttg aacttgttag caccttcttt tgctgtcttc
     5161 ttggttaaag ctatgtacgg taagggacaa acagacaaca ttcaacaatc ttttgggaag
     5221 tttgatttcc caggtttatc acaaattcct gtgattggtg atattttctt taaaaatact
     5281 agccttattg gttacttcgc cattgctttt tcgttctttg cttggttttt gctttataaa
     5341 accaggtttg gtctgcgttt acgatcagtt ggggaacacc ctcaggcagc tgacacactt
     5401 ggtattaacg tctatttgat gaaatactat ggtgtgatga tctcaggttt tcttggtgga
     5461 ataggtggag ctgtttatgc tcagtcaatc tccgttaact ttgctgtaac aactatctta
     5521 ggtcctggat ttattgcttt ggcagctatg atctttggta aatggaatcc agttggcgcc
     5581 atgctatcta gtcttttctt tggcttatcg caaagtttag ccgttattgg tgctcaatta
     5641 ccattactag aaaagattcc aacggtttac ttacagattg caccttatat ggtaacgatt
     5701 attattttag ctgctttctt tggtcaagca gttgcaccaa aagctgatgg aatcaactat
     5761 attaaatcta aataacgtta tattagtaag cctcttagtg aggtttaaca gcactcctaa
     5821 agcaagatag ttaggtctag ctttaagggt gcttttttgg gacttaccgc aagtttggat
     5881 tgccagtcac catcacaata gaagcaagaa tagactaaaa agaactaagt cagcggctta
     5941 gttctttttg cttgtctttt gtttttactg atagctctta agttgtttta gccaacaaag
     6001 gtattgattt ctgtttcaat gttagcaatt ttttcttgag cgatgtcaag agtatcacct
     6061 attgtggcaa tatagaactt gatttttggt tcagttcctg aagggcgaac tgcaatccat
     6121 gaatcatcag ctaacgtgta cttcaaaaca ttacttggag gagttgttaa gttagatatt
     6181 gttccatcat ttttagtagc ggtttgtttt tggaaatctt ctaataatac aatatcagta
     6241 ttgttaaatt gttttggacc attttcacgg aatttgtcca taatcttctt aatttcagca
     6301 gctccatcca cacctgaaag tgttactgaa atcgtttttt cagcaaaata gccatattct
     6361 ttgtaaattt catcaatacc atctgcaagt gttaaaccac gcgaacggta ataagctgcg
     6421 atctcagcaa ccaaaagaac tgcttgaata gcgtctttat cacggacaaa tggtttaata
     6481 agatatccga agctttcttc aaaaccaaac atataggtgt ggttatgttt ttcttcaaac
     6541 tcttgaattt tctcagcgat gaatttgaat ccagttaaaa cgttaaacat tgtagcgcca
     6601 taactttcgg caatttttgt tactaattct gttgatacga ttgatttagc gagagcagca
     6661 ttttctggaa gtgttcctgc tgttttatga gcttccagga tgtatttcgc aataatcgct
     6721 ccaatttgat ttccagaaag gtttttataa gaaccatctg gttggcgaat ttcaacgcct
     6781 aggcggtctg catcaggatc agtagctact aagacatctg cctcaacttc tcgtccgagt
     6841 tcttcagcaa gagcaaaggc agcttggctt tcagggttag gcgatttaac agtagaaaag
     6901 tctggatctg ccttagcttg tgattcgact acaacaacag attcaaaacc ggcttgagca
     6961 agggcacggc gtgtcaacat ctcaccagtt ccgtggagag gagtatagac aattttcatg
     7021 tcttttccaa agttattgat taaatcttga ttgatattaa catctttaac ctctctaagg
     7081 tattccatat caacatcttc accgataact tgaataagac ctgatgattt gttttcatct
     7141 aaatcagcaa gttcaactgc aaatggattt tcaatagcgc ggataaagtc tgttagtgca
     7201 tcagcatcag ctggtggtag ttgacctccg tcttgtccat agactttata accattgaaa
     7261 ggtgctggat tgtggcttgc agtaaccata ataccagcat aggcatttaa atggcgaaca
     7321 gcaaaagaga gttctggtgt tggacgaaga gcttcaaata cgtaagattt aattccatgc
     7381 tgtgccaaga cctgtgcaga ctcaaaagca aattctggag agaagtggcg cgaatcataa
     7441 gcgatagcta caccacgttt tttagcttcc tctcccttag tttcgattaa ttttgctaat
     7501 ccttcggttg cttgacggac aacataaata ttaatacgat ttgtaccagc accaatataa
     7561 ccacgcatac cagcagtacc aaattctaag tttgtgtaaa aagcatcttc ttttgttttt
     7621 tcatccattg atagtaattc ttgacgaaga tagtcaggaa gctgctcaaa atctaaccat
     7681 ttttgaaaat tttctgtgta agtcattgaa atactccttc aatatttata aacgctttca
     7741 ttatatcatt ttgaagggaa aaaaacacta attatttgca gctgtttaca aaactttttc
     7801 catattttca agtagctaat gtaagaaagt taaaataacc aagtttttaa aagagaaaaa
     7861 ctcggttatt gtgaaagcta attacctttt tagtcttgat agtgttggaa taagtacaaa
     7921 ggtaataata gctgagatga tcatctcaac aatagcgttt gatgagatga tggctgttag
     7981 tagtgcttta atatttccat caaaaactgt cgcgaaaaaa ataaatatcc cagataatac
     8041 aaatatagta tttgttagag aaccaatgat accggatact attaaaccaa actgattagg
     8101 aatcaattta tagcaataat aaggggtaat accaatcaga atacgtggga taatagcaat
     8161 tattaaagaa gcgaaagttc catgatcgac aaaaggtgaa aagagataat ttgtaggcag
     8221 tagaatgatg gtatttgtaa tgacacttat aatccccatt aatcctccta aaatagcacc
     8281 tatccttggg ccatataaga cagaagcaat aataactggg atatggacaa gcgttggttt
     8341 gattggaata ggccaaatat taaagacaag actgctcaca aaatgaatga ctaacatgat
     8401 ggcaaaaaag atagcaatac gagaaatatc tgctgattta cggtgattca ttttactctc
     8461 caaaaaatat aatacttaat tctaaaaagg tgatcagaaa aacgactgtt aaagtttcca
     8521 aatcgtatca atagtagcta agataacatc aatatcagcc aaagcaccag gtcctttatc
     8581 tccacaggct aataagctag attttggagg aatttctgta aaccctattg tcgataagcg
     8641 tttaatattt tcttgagtaa tagggttttg atacattttg gtattcatgg ctggggctat
     8701 taattttggt gtggtagctg gtagggcaag agcaacactt gttacaagat tatcagcgaa
     8761 tccatatgct agatgagcaa tagtattagc agaggcaggt gcaacaataa ataaatctgt
     8821 gcgttttgct aattcgatat ggttgataac ttttgggtca tgctcatcca taacatctaa
     8881 atgaatagca ttttttgata acacttgcag ggttaatggt gtaataaatt gtgtagcagc
     8941 ctgagtcatt atgatgtgaa catcatagcc aattttagtt aattgactcg taagatctgc
     9001 tgctttatag gcagatatac taccagaaac agctaaggta atatgtttag tcatgtttgg
     9061 tcatcctttc atataataag tcagcaattg cctctttggt atctgcagat gcaacttcgt
     9121 tattggatat caaaagagcc ttgtgcatac ctgtttggat atcaactaaa tcattggcga
     9181 gaatataatc agcgtgattt ttagctagac ttgctctggc tactttaatt aactcttctt
     9241 gaggcacatt aactagaagt ttaaagccga ctagtttaat attaggattc cactgtttaa
     9301 cataagagat aactttgggt gtttttttca aaaaaagaac ctgataatca gatgcagatg
     9361 agattttcgg ctcactgtta tgttcacata gaaaacaatt aaggttatca gcttgagaga
     9421 cttgttccaa atcagtcatg tagactggag tatagtcaga aacagccata ctatgaatta
     9481 gaatgtcatg gtgaggaacc tgatctttga gtgcagccat taagtcgtta actgtttcca
     9541 cttcaataat ccttaggcgc ttatttggca ggggtttagt ggctgtttta gtagtgacta
     9601 gggtaacgtc atgatgatat tgtaaaaatc gctctgtgat taattttcca agttgcccag
     9661 ttgagtgatt agtgatacct cgtactgcat ctataggttc agttgttcca cctgatgtta
     9721 ttattaattt catagtcata ttttatcaaa aaaggagctg atagtaaacg gaagataatg
     9781 acgccagaca ttgtcttgcg tttgtattaa tatggaatag taatagattt ctagtaatta
     9841 aaacgaagtt ctggaaagca agacactata gtagctaaaa tctaaatttt aaggtaaaat
     9901 agagcaagcg gtttcaagtg tgaaaggaag aattgtgata actattagag atttagcgtc
     9961 actgccgatt tttgtttacg gtgatggaaa taaaaaagtt ccaggagctt tgagtccttt
    10021 tgtgtggaca gaggtgtttt taaaaactat caatcaagaa cctaatcaac tgatcttaca
    10081 tatttggccg atgacaagaa cagttatatt aggaatgtta gatagacagc ttccctactt
    10141 tgagctggct aaaacagaga ttggcaataa tggttatgtt cctgttactc gtaatattgg
    10201 cggtttggct gttgtcgctg atgatgggat tttgaatttt tcgcttgtta ttccagacca
    10261 tttttcggaa tctatcagta ttagtaatgc ttatttaata atggttgatg tcattagaga
    10321 gagtttttcg gattattatc agcgtattga atatcacgaa attaaaaatt cttattgccc
    10381 tggtaatttt gatcttagta ttgcaggccg caagtttgca ggtatcgccc agcgtcgtat
    10441 caaaaaggga attgttgtgt ctatttactt gagcgtttgt ggggatcaag cagcgcgtgg
    10501 tcaactcatc aaggattttt atgaggccgg aacacaagga gaagttacca aggttaatta
    10561 ccctcaaatt gatccagaat gcatggcaac tctttcagaa ctccttgaga cgccttttac
    10621 agtggcagaa gtccttgaac gtcttcgatt gaccttgcga cagcttggct tttccctcac
    10681 ggaaaaaagt ccagatcaag ccttactgac taattttgat gctgtctatg aacggatgca
    10741 gctagaagtc gtcagaaagg aaggcaaatg aaagaaagat ttagtccttt atttgagcct
    10801 ttgactttgc caaatgggag tcaattggat aatcgctttg ttttgtctcc tatggtgacc
    10861 aactcatcta caaaagacgg ttatgtcacc caagatgatg tatcctatgc tttgagacga
    10921 gcagcttctg cccctctaca aattacagga gcggcctatg tggatcctta tggtcaattg
    10981 tttgaatatg gttttagtgt tacaaaagat gccgatattt ctggcttgaa agagctagct
    11041 caagcgatga aggcaaaggg agctaaagtt gtcttacaac tgacccacgc tggccggttt
    11101 gctagccatg ccttgactaa atatggcttt gtctatgggc ctagttacat gcagttgaga
    11161 tctccccaac ctcatgaagt caaaccttta acaggtcagc aaatagagga attgattgca
    11221 gcttacgctc aggccactcg gcgggcaatt caagcaggct ttgatggcgt ggaagtatct
    11281 tcggcgcagc gcttattgat tcaaacgttc ttttcgactt tttcgaacaa gaggacagat
    11341 agttatggtt gtcaaacact ttttaatcgc tctaagttaa ctctagctgt tttgcaagct
    11401 gttcaacagg tgattaaaca agaagctcct gatgggttta tttttggatt tcgtgctaca
    11461 ccagaagaaa ctaggggaaa tgatattggt tatagtattg acgagttttt gcagttgatg
    11521 gattgggtac ttaatgttgc aaaacttgat tatttagcga tagcaagttg gggccgtcat
    11581 gtatttcgaa atactgttcg ttctcctggc ccttattatg gaaggcgagt taatcaggta
    11641 gttagagatt atttaagaaa taagctacct gttatggcta cagggggaat gaacacacct
    11701 gacaaagcta ttgaagcact agctcatgct gattttattg gagtatctac accttttgtc
    11761 gtggatccag aatttgctca taaaatcaaa gaagggtgtg aagagagtat tcatttaagg
    11821 attcgacctg ctgatttgaa aagtttagcg attcctcagg cttctttcaa agatattgta
    11881 cctttgatgg actacgggga atcattgcct aaagaatcaa gaactttatt tcgtagtctt
    11941 actcacaatt ataaggagat taaatgaaag tcagtattct tgattatggg gtgattgata
    12001 aagaaaaaac accccaagaa gctttattag aaacgcgctg cttagcacaa gtagccgaca
    12061 aacttggttt tcatcgtttt tgggtagcag agcatcataa tatttatgcc tttgcaataa
    12121 gcagtccaga gctgttgatg atgcatttag cagatcatac caaacagatt cggataggtt
    12181 caggaggtat tatgcctcta cattatagta gctttaagat tgcagaatgg ataatgactt
    12241 tagaggcctt gcaccctaat cggattgatt taggtattgg caactcttta ggaactacgt
    12301 tagttcaaag agctctatca agtatccatt gcaaagacag ctatagtcag gttgtcacag
    12361 aattatacca gtatcttaat cctgatcatt tgtcaccact accgattttt gttaacccta
    12421 ggggtaatac ttaccctcag atttggacgc taagcaatag tttggagaca gcagaactgg
    12481 ctggtcaatt aggtttaggc tatacctttg gtattttccc ctatatacca aaagatccta
    12541 taacagaagc taaaagagta tcggcacatt atagaaaggc ttttaggcct tcaaagctat
    12601 taaaaatccc caagttaata ttagcagttt ttattgtcct ttctgatacc gacgagaaag
    12661 cagaagcttt ggctaaaccg ttggacattt ggatgcttgg tcagcaagat tttaatgaat
    12721 ttaagaccta ccctgatgtt gaggaggcta gaaactatca cttaacagag aaacaaagag
    12781 aagccattgc agcaaatcgt agtcgcatgg ttattggatc acctcacaca gtcaagaaac
    12841 aattggatag gctgatagaa gcttgtcaag ctgacgaatt attagccatc cccttagttc
    12901 ctgaatttgc caatagacaa cgaaccttag agctgctagc agacttatac gtgaccattt
    12961 aaccttacca aaaacaattc cctaaactga aaggaaagaa acaatgaaaa aaattgcaaa
    13021 ttacttatta attgagaaaa cagatgaccg ctataccatt agcatgacac cagaattaca
    13081 agacgatatt ggtactattg gttacgctga atttacggat aacgaccacc ttgccgttga
    13141 tgacattatc ttaaatctag aggcttctaa gactgtcatg tctgtcttgt caccattggc
    13201 tggggcagtt gttgagcgca atgaggcagc cacactaaca ccaacccttt tgaattcaga
    13261 aaaagctgag gaaaattgga ttgttgtgct aacagatgtt gatcaagcag cttttgatgc
    13321 cctagaagat gcctagttca tttgatttat taggggaaat gattggtctg ttgcaaacag
    13381 agcagcttac tagttcttgg gcctgtccct tgcctaatgc tttaacaaag cgacaagact
    13441 tgtggcgggc gctcattaac cagcgccctg ctttgccctt atcaaaagat tacttgaatc
    13501 tggaagatgc ttatctagat gattggcgtg cttcttttgt tcctgtatca gtcaaggatt
    13561 gccaaaaaac taattatacc agtctttttc tttaccatgg ggatattagg tatcttgctg
    13621 tggatgctat tgtcaacgct gctaatagtg agttattggg atgttttagt cctaaccatg
    13681 gctgtattga taatgcgatt catacgtttg ccggaagtcg tttacgctta gcttgtcagg
    13741 caatcatgac tgaacaaggc cgtaaggaag ccattggaca agcaaagctt accagtgctt
    13801 atcatctacc agcctcttat attattcata ccgtagggcc aagaattacc aaaggacacc
    13861 acgtttctcc gattcgagct gatttgttag cacgttgtta tcgttctagt cttgatttgg
    13921 cggttaaagc aggattgact agcctagctt tttgctctat ttcaacagga gaatttggct
    13981 ttcctaaaaa agaagccgct cagattgcta ttaagacagt tttaaaatgg caagcagaac
    14041 atccagaatc taagacctta actaccattt ttaatacctt tacatcagaa gacaaggcct
    14101 tatacgacac ttatttgcaa aaggagaata actgtgagta attggacaac ctatcctcag
    14161 aaaaacctca cccaggcaga acaactggcc caattaatca aagaagcaga tgcacttgta
    14221 gttggtattg gagctggcat gtctgcagct gatggtttta cttatatcgg gtcgcgtttt
    14281 gagacggcat tcccagattt tattgctaag tatcagtttt tggatatgct gcaagctagc
    14341 ctttttgatt ttgaagattg gcaagaatat tgggcttttc aaagtcgctt tgtcgctttg
    14401 aattatcttg atcagcccgt aggccagtct tatcttgatt taaaagagat tttgggaacc
    14461 aaagactatc atattattac tactaatgct gataatgctt tttgggtggc tggttatgat
    14521 cctcataaca tttttcacat tcaaggagaa tatggtttgt ggcaatgtag ccagcactgc
    14581 catcagcaaa cttataagga cgatacggtc atccgtcaaa tgatcgcaga gcaaaaaaac
    14641 atgaaagtcc caggtcaact gattcctcat tgccctgaat gtgaggcgcc ttttgaaatc
    14701 aataagcgta atgaagaaaa aggaatggtt gaagatgctg actttcatgc ccaaaaagca
    14761 cgttatgagg cttttttatc agaacataaa gagggtaaag tgctttatct agaaattggt
    14821 gtgggacata cgacgcctca gtttatcaaa cacccctttt ggaaacgtgt ctcagaaaat
    14881 cctaatgcct tattcgttac cttgaatcac aaacattacc gcatccctct gtcaataagg
    14941 cgacaaagcc tagaactgac agaacacatt gcacaattga ttagcgccac aaaaaccatt
    15001 taccaaaaat cgtaaccaaa gccaatagac ggttaattaa gagatagaga aggagactaa
    15061 catgtacctt attgaaccga ttcgtaacgg taaacgtatc accgatggag ctgttgctct
    15121 agccatgcaa gtttatgttc aagaaaatct atttttagat gatgatattt tatttcccta
    15181 ctattgtgat cccaaagttg aaattggaaa attccagaat gcagttgtag aaactaatca
    15241 agagtattta aaagagcatc atatccctgt tgttcgcaga gatactggcg gtggtgccgt
    15301 ttatgtagat tctggtgctg ttaatatttg ctacttgatt aatgataatg gtatttttgg
    15361 tgattttaaa agaacctatc agcccgctat tgaagccctt catcatttgg gagcaacaga
    15421 ggttgaaatg tctggacgga atgacttggt tattgatggg aaaaaggtat ctggcgctgc
    15481 gatgactatt gctaatggcc gtgtttatgg gggatattcc cttttgctag atgtggactt
    15541 tgaagccatg gaaaaagcat taaaacctaa tcgcaaaaaa attgaatcaa aaggcatcag
    15601 gtcagttcgt agtcgcgttg gaaacatcag agaacattta gcaccacaat accaaggtat
    15661 tacgattgaa gagttcaaag atttaatggt ttgccaattg ttacagattg agacgattag
    15721 ccaagctaag cgttatgatt taacggaaaa agactggcaa caaattgatg ctttaacaga
    15781 gcgcaaatat cacaattggg aatggaatta tggcaacgcg cctcaatacc gttaccatag
    15841 agatggccgt tttaccggtg gaacagtaga tattcattta gatattaaaa aaggctatat
    15901 tgcagcttgt cgcatttacg gagatttttt tggcaaggca gacattgcag aacttgaagg
    15961 tcacttgata ggaacacgaa tggaaaaaga ggatgtctta gctaccctca atgcaattga
    16021 tttagcccct tatttaggtg cgattacagc agaagaatta ggagatttga tattttcttg
    16081 ataaaaaaga tacctcagac cggtaaagtg tgattaaaaa cgaacaataa ttgttttaat
    16141 tcaaataaat gtacgtatga tatgaattgt gatataatga cgtcattacg taaaattgag
    16201 gagtcatcat gaaatcagat atcgaaattg cccaaagtgt cgccttacaa ccaatcactg
    16261 acattgttaa aaaggttgga atagacggcg atgacattga gctttatggc aaatataagg
    16321 ctaaattgtc ttttgagaaa atgaaagctg tcgaagctaa tgagcctgga aaattaatct
    16381 tggtaactgc tattaaccct actccagcag gagaaggaaa atcaaccatg tctattggac
    16441 tggcagatgc cttaaatcaa atgggaaaaa aaaccatgct tgccttacgt gaaccctccc
    16501 ttggtcctgt tatggggatt aaaggcgggg cagcaggtgg tggttatgcc caagtattgc
    16561 ctatggaaga cattaatctt cactttactg gtgacatgca tgccattaca accgctaata
    16621 atgcattatc tgctttgatt gacaatcatt tgcagcaagg aaacgattta ggaattgatc
    16681 ctcgccgcat tatttggaag cgcgtgttgg accttaatga tcgtgctctt cgtcaggtaa
    16741 ttgttggctt aggtagtcca gtgaatggtg tgccacgcga agatgggttc gatatcacag
    16801 tagcttctga aatcatggca attttatgtt tggcaactga cttaaaagat ctaaagaaac
    16861 gtcttgctga tatcgttgtg gcttatacct atgatcgtaa gcctgtctat gtgcgtgact
    16921 tgaaggttga aggagccttg acattaattc taaaagatgc tatcaagcca aacttggtgc
    16981 agactattta tggaacacca gctcttattc atggggggcc atttgctaat attgctcatg
    17041 gttgtaattc tgtccttgct acctcaacag ctttgcgatt agcagattac actgttaccg
    17101 aagcaggatt cggggccgac ctaggagctg aaaaattcct aaatatcaaa gtacctaatc
    17161 ttcctaaagc accagatgct atcgttattg ttgcaacctt gcgcgccctt aaaatgcatg
    17221 gaggtgtcgc caaatcagat ttagccgctg aaaattgtga ggctgttcgc ttaggatttg
    17281 ccaacttaaa acgtcacgtg gaaaacatgc gccaattcaa agttccagtt gtggtggcta
    17341 tcaatgaatt tgtagcagat accgaggctg aaattgctac cttaaaagcg ctttgtgaag
    17401 agattaaggt tccagtagaa ttagccagtg tttgggcaaa tggagctgag ggaggcttag
    17461 cattagctaa aacagtggtt cgtgtgattg atcaagaagc tgctgactac aaacgtctct
    17521 attcagatga ggatactttg gaagaaaaag tgatcaatat cgttacccag atttatggtg
    17581 gcaaagctgt tcaatttggc cccaaggcca aaacacaatt gaagcagttc gcagaattcg
    17641 gttgggataa attgcctgtt tgtatggcta agacccaata cagtttttca gataacccat
    17701 ccttgcttgg agcgccaacc gattttgaca ttaccattcg tgaatttgtt cctaaaacag
    17761 gagcaggttt tattgttggt ttaacaggtg atgtgatgac catgcctggt ttgcctaaag
    17821 ttccagctgc catggcaatg gatgtagcag aaaatggcac agccctcggt ctcttttagg
    17881 aaaaattgct cgaataattc gagcaagcgc tcctttcttc ccgctttact gtgatataat
    17941 aaggtaaatg agattttgga gggatgatta tcaaaaagaa agcaaaagta aaatatttgc
    18001 tccataaagg gaaacatgga tttttaagag gaatttttag tagaacaaca atcattgtat
    18061 tattaattat tttgcaatta gttttcttat ttcaatctta tgcctggatg gagcagtacc
    18121 gtgtttggat aacgattctt gaaagtgtct ttgctattac tattgtttta tatttagtta
    18181 atagtgatat ggatgctatt tcaagaatga cgtggttaat tttgattatg attgctccat
    18241 tactagggtc actgttttta atttatacaa aattggattg gggttacaga gggctaaaac
    18301 aaagaataaa tcatcttgta gatctgtctg caccttacct tagcgatgat gacgctatat
    18361 tagaggtatt aaaggatagc acatcaacca catatcattt agtacagtac ttagaaagaa
    18421 gtcgtggcaa ctttccgatt tataataata caagggtaac ttacttccca acaggtgaaa
    18481 ctttttttga tagtttaaaa gaacagttat tcttagctaa aaagtacatt tttcttgaat
    18541 tttttattat tgcagaaggc caaatgtggg gagaaatcct tagtattcta gaaaaaaaag
    18601 tcagtgaagg tgtagaagtt agggtgttgt ttgatggcat gaacgaacta tcaacgctat
    18661 catcagatta cgccaagaga ttagaacaaa tagggattaa agctaaatca tttttaccga
    18721 tttcaccctt tatctctacc tattacaatt atcgagatca ccgaaaaatt gtcgttattg
    18781 atggggaggt atcattcact ggaggtatta atctagcaga tgagtacatt aatgaagtag
    18841 agcgttttgg ccactggaaa gatgctggtt taatgcttga gggtgaagca acagacagct
    18901 ttttaatttt gtttttacaa atgtggtcta tcacagaaaa agaactgatt attgatcctt
    18961 atctttcaga tcattcttta aaacttcctt cagatggcta tgttattccc tacggtgatt
    19021 ccccgcttga tactgataaa ataggtaaaa atgtttatat agacatttta aatcatgcta
    19081 aagagtacgt ttatatcatg acaccttacc ttattttaga tagcgagatg gaacacgctt
    19141 tacggtttgc atcagaacgt ggagtagata tccgaattat tatgccagga gtgccagata
    19201 agggggtacc atatgcctta gctaaaacct attataaagc tttgatgtct tctggagtaa
    19261 aaatttatga gtatcaacca gggttcgttc attcaaaggt atttatctct gataatacaa
    19321 aagcagttgt cggaacaatt aacttagact atcgtagcct ttatcatcat tttgaatgcg
    19381 cgacttattt atatcgtgtg tcagttattg ctgatattgt gaatgacttt aatgaagctc
    19441 aaaagcaatc actattgatg acatcagatc atttgacgca acgtccttgg tatcaaaaat
    19501 tgataggatt attagtacga acaattgccc ctctcttgta attttcgtaa aatatgttat
    19561 aatatctgta tgcgatgagt cgattgtgac tgcaaagtca cttttgcgca ggggaggtca
    19621 ttaaggcggg aagccgactt tgataatctg tgtgaaccat tttatcacaa tcttatttat
    19681 taagaatgcc ccttggattg agatattctc aatccttttt ttaattttat tatcttagtt
    19741 aaaattcaga atgtatcagt gattactgtg tttcaaaata agaaagtagt ttttcgggta
    19801 tgagtgctat gacttgacaa atgctaataa catttgcata atgtaggtag agatgaaaac
    19861 ttatgaaaag agatgatctt atgcaaatta ttaagcgtga tggccacgtt gctgattttg
    19921 atccagataa aatttatcaa gcaattatta aagcagcaag gacggtctat gttattgatg
    19981 acacgtggcg acaaaactta acacatgtca ctaaaaaagt agttattgat ttagaagaag
    20041 cacatgtaga acgtcctact atcaatatgg tccaatccct tgtggagaat cgtttgatgg
    20101 atgctggata tattaccatt gcagaacatt acatttctta tcgtcttcag cgagatttag
    20161 agcgcaatgg ctatggtgat aagattgtgg ttcacttgcg ttttgaacaa ataaaataaa
    20221 aacgcttcta atggcatttt agtgtctttg aggggataag gtttaaatac ctaacagata
    20281 aaaatctgtt aggtattttt ttgctactat taggtgtatt ctatattaca tagaacttaa
    20341 gcattcaagc tttagtttaa ctttctgttt attttttgct acattttggg aaactcccct
    20401 cctttcttag ttgaggtgaa taaactttta tttgataaat tatatcctta gtcttaatga
    20461 aacagtctag gataatacta cttcgaagtg acagctagta gtttccaatt tttaagtagt
    20521 ctaaaattat gtttttttaa tttcaaaacc atgcttttta gagattttat aatacttatt
    20581 tatcc
//
LOCUS       DAXWIQ010000034        19544 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000034 DAXWIQ010000000
VERSION     DAXWIQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19544
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.034"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..183
                     /locus_tag="ACGEUI_001258"
     CDS             <1..183
                     /locus_tag="ACGEUI_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFF8166096.1"
                     /translation="DSLTRQVKNIYFLQNTKDDMLSFSDINVMPRTQDIEREFNVKEW
                     IGDGIEHIENPLIEWI"
     gene            451..870
                     /locus_tag="ACGEUI_001259"
     CDS             451..870
                     /locus_tag="ACGEUI_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFF8166097.1"
                     /translation="MSKAKAILKDLRNLDLYIASLIRRRDKIEASLLSSPKWSSDKVN
                     GGIKRKQDDVYVELIATAKDIEKKTAEAIRKQRELQNLIDSLENTDSQTILSMVYIDK
                     MTRWQVIDELNCSESTYFRLLRVATKELNNLTVNDSD"
     gene            979..1323
                     /locus_tag="ACGEUI_001260"
     CDS             979..1323
                     /locus_tag="ACGEUI_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFF8166098.1"
                     /translation="MSQLRADKKGTHRVAFDRNKKKLLKAATVCGICGKPVDKSLKYP
                     HPLSAAIDHIVPIAKGGHPSALENLQLTHWQCNRQKSDKLFANQASNEPKTIGNRNLP
                     QSRDWSSFAFKK"
     gene            1472..1827
                     /locus_tag="ACGEUI_001261"
                     /pseudo
     CDS             1472..1827
                     /locus_tag="ACGEUI_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1824..3092
                     /locus_tag="ACGEUI_001262"
     CDS             1824..3092
                     /locus_tag="ACGEUI_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFF8166099.1"
                     /translation="MNYMGMGYLRRKLALFKTGVDKRYRYYAMDDRDDTRSIVMPNNV
                     REMYRSVLEWTAKGVDSLADRIIFREFTNDDFNAWEIFKANNPDIFFDTAIQSALIAS
                     CCFVYIMPGAEDGLPKMQVIEASKATGILDPTTFLLTEGYAILESDSNGNPTLEAYFT
                     DKDIWYYPKKGKPYNIKNPTGHPLLVPIIHRPDAVRPFGRSRITKAGMYHQKAAKRTL
                     ERAEVTAEFYSFPQKYVLGMDPDAEPMEKWRATVSTLLEISKDEDGDKPTVGQFTTAS
                     MAPFMEHLKMYASLFAGGSGLTLDDLGFPSDNPSSVESIKAAHENLRAAGRKAQRSFS
                     SGFLNVAYIAVCLRDEFPYLRNQFMDTVIKWEPLFEADANMLTLVGDGAIKLNQAIPG
                     FMDADVIRDLTGVKGADKPIPAITEVTTDG"
     gene            3085..4578
                     /locus_tag="ACGEUI_001263"
     CDS             3085..4578
                     /locus_tag="ACGEUI_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166100.1"
                     /translation="MVDDVLPKLLKSVRQDFEKYFGESDVVTKAFAELQAKKVTYKTV
                     NEFAIEVGRLLSLALTGSVSSDKLPDGKMYYNIAKRLLDETMGRNYKLISGYAGDVQR
                     ILNENAQIGLKVQRPPLNRDKINRMVNRLDSENTFDDVKWLFGEPIVNFSQSIVDDTI
                     KANADLQYKTGMTPQVVRTESGNCCEWCREVVGTYSYPKVPKDVWRRHQRCRCTLDYD
                     PKNGKVQSAWSKIWRKKEKTQESIERVEKFKESALVESIKNDIAKLDMTKVGPSDIID
                     IGKRINYHFRVSEHIGDKEKLKEIFSNFREIGGEIPKNTWAKGSSKLVKDQLQEAFQN
                     YPTEWAAVPDGIGKKLKAIKRKRGYFDGYDEDLVIATNGTRKTTPYHEIGHMIELVNP
                     DLVRLEKAWVDKRTANEAEVRLKDIFPSSNYGIGEVTKKDDFISPYIGKYYSDAAEVF
                     TMGLQGIFVPEERFAKSFDKKTWKYDYKTINDDPEFLNFIIGLFVKV"
     gene            4584..4808
                     /locus_tag="ACGEUI_001264"
     CDS             4584..4808
                     /locus_tag="ACGEUI_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166101.1"
                     /translation="MEPKLHRQLRQKYDDAEKQYLEKFGEDSLDRVFFWEPDVYFDEW
                     KKVLPDATLELNKAINSGVAIGPDPENAIY"
     gene            4858..5037
                     /locus_tag="ACGEUI_001265"
     CDS             4858..5037
                     /locus_tag="ACGEUI_001265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166102.1"
                     /translation="MNKISLNVEMTWENKDEFQQIMNKVNETKEAYEKALEAAENFVP
                     KMSCATKLNKGEGDE"
     gene            5030..5296
                     /locus_tag="ACGEUI_001266"
     CDS             5030..5296
                     /locus_tag="ACGEUI_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166103.1"
                     /translation="MNKRIKKKRKLERAIVMLVAENAMQAEAIKNQNKEIMELKSIVQ
                     RNALATNEEFASVKAATLDNQSAIADIAIDVNYIKKNYKRKWGK"
     gene            5406..6821
                     /locus_tag="ACGEUI_001267"
     CDS             5406..6821
                     /locus_tag="ACGEUI_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFF8166104.1"
                     /translation="MVTKTKTKLGNQRPTQSVNLHFAKSLAHEAINYYKKTGLSCYPW
                     QVNMLIPIMAIDENGLWVHQKYGYAIPRRNGKTEVVYIVELWALHKGLKILHTAHRIS
                     TSHASFEKVKKYLEMSGYVDGEDFISNKAKGQERIEFKASGAVIQFRTRTSNGGLGEG
                     FDLLIIDEAQEYTSEQESALKYTVTDSDNPMTIMCGTPPTMVSTGTVFEAYRKDCLKG
                     NKRYSGWAEWSVPEMVKINDVSSWYISNPSMGFHLNERKIEAELGEDEIDHNIQRLGY
                     WPSFNQKSVISEKEWAKLKVEQVPELKSKLFVGIKFGQDGNNVSLSIAARTSENKVFV
                     ETIDCLSVRNGTQWIINFLKSADIAKVVIDGASGQELLAQEMKDQGLKKPELPKVAEI
                     ITANMMWEQGIMQETICHSDQPSLTAVVTNCEKRQIGSNGGFGYKSLYDDRDISLMDS
                     ALLAHWICYTTKPKRKQRTSC"
     gene            6902..7363
                     /locus_tag="ACGEUI_001268"
     CDS             6902..7363
                     /locus_tag="ACGEUI_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFF8166105.1"
                     /translation="MSEFKVIETQEELDTIVKARIAREREKYQDYDQLKTRVEELETE
                     NSSLQTALNDAKSNTDSYTEEISTLKNQIADYEAANLRTKVALQYGLPIDLADRLQGD
                     DEDGLKVDAERLASFIKPSQPQPPAKSNEPNIDSNADANYRALVQGLSTED"
     gene            7388..8299
                     /locus_tag="ACGEUI_001269"
     CDS             7388..8299
                     /locus_tag="ACGEUI_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFF8166106.1"
                     /translation="MGTETSKASLFDKHLVSDLINKVKGHSSLAKLSSQKPIPFNGSK
                     EFTFTLDSDIDVVAENGKKTHGGLSLEPVTIVPIKVEYGARLSDEFLYATEEEKIDIL
                     KAFNEGFAKKLARGIDLMAMHGINPRTKKASDVIGTNHFDSKVTQVVKFTESEDADAN
                     IEAAVNLIQGSEGVVTGLAMDTEFSTALAKVTNGEMGPKMYPELAWGANPDSINGLKS
                     SVNTTVGAGADEAESKDLVIIGDFESMFKWGYAKQIPMEIIKYGDPDNSGKDLKGYNQ
                     IYLRAEAYIGWGILDAKSFARVTKGEV"
     gene            8299..8499
                     /locus_tag="ACGEUI_001270"
     CDS             8299..8499
                     /locus_tag="ACGEUI_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166107.1"
                     /translation="MLYVNSKTGASFYSSVPVCGGDWTLVEQQTGEKAKTVAEIKRQL
                     DAAGIKYSSKAKKPELEALLPG"
     gene            8509..8931
                     /locus_tag="ACGEUI_001271"
     CDS             8509..8931
                     /locus_tag="ACGEUI_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp19/Gp15/Gp42 family protein"
                     /protein_id="HFF8166108.1"
                     /translation="MGNFATTDDVILLWRPLSVDELKRANALLKVVSDTLRMEADKVG
                     KDLDKTMVDKPYFVNVIKSVTVDIVARTLMTSTRGEPMAQESQSALGYTWSGTYLVPG
                     GGLFIKDSELKRLGLKKQRYGGIELYGEIERDNSCFSR"
     gene            8891..9229
                     /locus_tag="ACGEUI_001272"
     CDS             8891..9229
                     /locus_tag="ACGEUI_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166109.1"
                     /translation="MAKLKGITVALADKSISGKDPFGNPVTVDFDIKIENVLVAPATT
                     EDITNQLSLTGKKVEYVLAIPKGDEHDWENKEVRFFGKKWRTVGIPLEGIEELIPLDW
                     NKKVMVERYE"
     gene            9222..9458
                     /locus_tag="ACGEUI_001273"
     CDS             9222..9458
                     /locus_tag="ACGEUI_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166110.1"
                     /translation="MSKFKFKLNRAGVAELMKSSEMQQVLTTKATAIRERCGDGYVQD
                     IHVGKNRANAMVSTKTIKAKKDNSKNNTLLKAVR"
     gene            9459..9794
                     /locus_tag="ACGEUI_001274"
     CDS             9459..9794
                     /locus_tag="ACGEUI_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166111.1"
                     /translation="MIEVIIKKYLDEHLDVPSFFEHQEDEPARFIILEKTSGAKQNHL
                     LSSTFAFQSYAESLYEAALLNDKVKQVIEQLDVLPQVSGVHLNADYNFTDTATKRYRY
                     QAVFDINHY"
     gene            9806..10399
                     /locus_tag="ACGEUI_001275"
     CDS             9806..10399
                     /locus_tag="ACGEUI_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8166112.1"
                     /translation="MNKNDTKNVTSAKPKTGGAIYSAPLGTELPKDAKSELNTKFKNL
                     GYVSEDGVVNEDTRSSENIKAWGGDIVGAVQTEKEDKFTYKLIESLNVEVLKEVYGAV
                     NVTGDLSGGIQIKSNSKELEAHVIVVDMIMNGGILKRIVLPNAKVDEVGEIKYVDGEV
                     VGYETTLKCFPDKDGDTHREYIVKPGEANKKENSFEM"
     gene            10410..10673
                     /locus_tag="ACGEUI_001276"
     CDS             10410..10673
                     /locus_tag="ACGEUI_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166113.1"
                     /translation="MEILKGKTTSGFEYEIPKKRLKNFELVEAIAEEETDPTAVVKIV
                     NLLLGDAAKSLKEHVRDAEGIVDVEAIGVEIKEIFESQKDLKN"
     gene            10688..11059
                     /locus_tag="ACGEUI_001277"
     CDS             10688..11059
                     /locus_tag="ACGEUI_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5361 domain-containing protein"
                     /protein_id="HFF8166114.1"
                     /translation="MIAKDDDALTCDLAETYGIYDYRQLPAYQVAVFAVGLRSNSRIK
                     MALSGETEALDTVLLAGIYDNTNLLFWSKTKDGQSGQNKPKSVVEAISGSKSQKANDV
                     ISFVSGEDFENARKQLLGGDG"
     gene            11059..13416
                     /locus_tag="ACGEUI_001278"
     CDS             11059..13416
                     /locus_tag="ACGEUI_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166115.1"
                     /translation="MATELGQAYVQIMPSARGISGAISKQLDPEARSAGLSAGSLIGG
                     NLVKMIGGAIAAAGIGKMISSALSAGADLQQSFGGIDTLYKGAETAVKGFAKEAYKAG
                     ISANTYAEQAVSMGASLKQSLGGDAVAAAKAANMAIMDMADNSAKMGTDITSIQMAYQ
                     GFAKQNYTMLDNLRLGYGGTKEEMKRLLSDAEKLPAAMGKKFDLSNYADVVEAIHLVQ
                     DNMGIAGVAAEEAKTTFSGSLAAMKSSFTNVMAGLSLGDDIRPALRGLAETTSNFLFG
                     NFIPMVANIFKGLPSAIGTFIGAAAPIITSQFQGLMSSLGISIDLSPITAKFAQIGQN
                     LQPVFNGLKTAFSQLPSFFTSIGSAVAPVIDTIISGLARLDFSGFEALISAILPALQA
                     GFSNFAAIVGPAISGVVDSFVGMWNAAQPLISILSDALMPVFQILGSFLGGVVKGALM
                     GVSFAFDAVKVAIQLVTPIIDLLVQGLNFVQPVLSVIAEWIGVAIGMFGNLGTAGQGL
                     SAFIKSAWTNIQTAISTAGTIISTVIDYIKLAFSGAGSAVGVLKNIFSLAWMAMGDAI
                     NVAKGIISSVINGIKSAFSSFSSLVSSVGSAVNGVIDSISSTIRGLANIDISGAGAAI
                     MNGFLNGLKSAWGAVKSFVSGIANWIAEHKGPISYDRVLLKPAGKAIMGGLNTSLIDG
                     FKEVKSNVSGMADDLASTMTGKSLSLGIDAKPSVTADDLLSSNISTKTTVGSATSDLS
                     LFFVKVLALLQDILDKNTDVYLDKEKVSAILYEEFAKIMAREGIV"
     gene            13413..14108
                     /locus_tag="ACGEUI_001279"
     CDS             13413..14108
                     /locus_tag="ACGEUI_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166116.1"
                     /translation="MIPKVIIDDFDTSSIPNCVLTGYDVGDILSPSFVENEAYGMNGT
                     SRELESYNESKPTIMWHLSTFDDAVNLINHLDGLSKKIEFWHIPNSIYYYDCLSVKIN
                     AVTMSSWRVTLKLALYPFRYAKGVSDVVIAGNGNINNAGNVFSEPKIVVEGTGKGTLT
                     IGKQVMELNLSGKATIECKHGQQCVYDAEGNVKNSIRIRGSFFEIQPGTQGIAVSGGI
                     TRTIISPRWRYKV"
     gene            14105..15973
                     /locus_tag="ACGEUI_001280"
     CDS             14105..15973
                     /locus_tag="ACGEUI_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HFF8166117.1"
                     /translation="MISIKDDNTPLVAAFEDEITQEANSDYKLNFKYPAKHEYRPLIK
                     KGIILEADDLHGSQLFRIFEITKRHGYINVYANQVADDNTMHDFVKPKVTTQQAFGLA
                     QQVKELDLQLKDPKSDLWGKIKFNNKAMLVEYANKEMSSAIAQSAEQILLQVKSIDDE
                     RYSKFEQTLNGIKQTVKSESVESARTQLASMFDSRISGLDGKYSRLSQTIDSLSSRLD
                     DGVGNYSTLSQKVSGIDLRVSNAANDVSRLSQTAQGLQSQITNANQNYSSLSQTVQGL
                     QTTVRDNQSNATSRINQLSNLISTKVSKGDVETTIAQSYDKIAFAIRDKLPASKMSGS
                     EIISAINLDRSGVKITGKNITLDGNSYISNAVIKDAHIANMDAGKINTGYLNANRIAT
                     EAITGEKIKMDYAFFNKLTANEGYFRTLFAKDIFATSVQSVTLSASKITGGVLAATNG
                     ASQWDLNNANMTFNRDATINFNSKNNALVRKDGTHTAFVHFSNATPKGYRGSALYASI
                     GITSSGDGIDSASSGRFAGLRSFRYATGYNHTAAVDQTELYGDNVLIADDFSINRGFK
                     FRPHKMEKVLDMNDLYAAVVALGRCWGHLANVGWNTAHSNFTSAVSRELNNYITKI"
     gene            15985..16422
                     /locus_tag="ACGEUI_001281"
     CDS             15985..16422
                     /locus_tag="ACGEUI_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFF8166118.1"
                     /translation="MQLTIKNKDLNTLYRVLDKIKVTNMRANRGRAKLLAKVVDKFKE
                     YAKDEGDLIDLYAQKDKDGKFVIDEHKNIKLADPAKLDEFNGLLNELADEEIVIKGGE
                     YSKRFIDFLNFLEECEDEFTSSEIILIDNILEQFEESKKGEKP"
     gene            16419..17036
                     /locus_tag="ACGEUI_001282"
     CDS             16419..17036
                     /locus_tag="ACGEUI_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HFF8166119.1"
                     /translation="MRNWKVTGKYPQFDSTGAVASTHVIIAAEDGAVIPQLVKKDLTG
                     TNDKDIITAVLEEFKKSEYVEIAMGEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVS
                     AERTQRMINLQTIHMLTSGGKIDPDIYKGMLELIEPAKKGEYQAYDVFTVVDSTKEED
                     GEAGEGNLVFVHVNEAFEYDKQTLEELESEAKVTVIKYADLVKQD"
     gene            17046..17321
                     /locus_tag="ACGEUI_001283"
     CDS             17046..17321
                     /locus_tag="ACGEUI_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166120.1"
                     /translation="MATELIFGVGGFILAIVTTYNIFNAKSIKHATDITLLQSEVEHL
                     KIVTRQNARRLEEHAEQNKTLITMTEQIKNLTDDVKDLKDMMRGEIK"
     gene            17318..17545
                     /locus_tag="ACGEUI_001284"
     CDS             17318..17545
                     /locus_tag="ACGEUI_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFF8166121.1"
                     /translation="MINLKLRLQNKVTLMAILGAIFLLAQQLGIKLPSNIADIANTAV
                     TLLVLLGVVTDPTTKGLSDSEQALTYHEPKQ"
     gene            17661..18866
                     /locus_tag="ACGEUI_001285"
     CDS             17661..18866
                     /locus_tag="ACGEUI_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8166122.1"
                     /translation="MTFLDKIKQGCLDGWTTYKILPSLTAAQTILESGWGKHAPHNAL
                     FGIKADSSWTGKSFDTKTQEEYQAGVVTDIVDRFRAYDSWTDSIIDHGKFLNDNPRYQ
                     AVIGETDYKKACHAIKDAGYATASGYAELLIQLIEENDLQEWDEEAIVRKEKQMISSQ
                     CREVIEFFINLVNAGVGVDKDGFAGWQCTDVPCYAAKHWFGVDLWGNAIDLLDSAAAV
                     GWEVHRMPTDANPQTGAFFVQSVPYHQFGHTGVVIEDSDGYTMRTIEQNIDGNPDALY
                     VGAPARFNTRDFTGVTGWFYPPYQGDIVTQPVSTEPQTSDTIVETAKTGTFTLDVAEI
                     NIRRWPSLTSEVVGSYKQGDTISFDSEGYANGYYWISYVGGSGMRNYTAIGITDKDGN
                     IISLWGKLN"
     gene            complement(18936..19370)
                     /locus_tag="ACGEUI_001286"
     CDS             complement(18936..19370)
                     /locus_tag="ACGEUI_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166123.1"
                     /translation="MKRSKKASLLSVIVGMALAVIPIYIVNYDNELIVLLMSIFFNGI
                     IVSAFELIKTYDGLQEEYKKLTVDRWKNTILLAFNSLYFASILTNSLSNNLSTILSTN
                     RSDKVSAIFLTGCSILALLPFLLHRLIKREIEIENERKKLKK"
BASE COUNT         6546 a         3326 c         4289 g         5383 t
ORIGIN      
        1 gactctttaa caagacaagt taaaaacatt tattttttgc aaaatacaaa agacgacatg
       61 ctgagtttta gtgatattaa cgttatgcca aggactcaag atattgagcg agaatttaat
      121 gtcaaagagt ggattggtga tgggatagaa cacattgaaa atccactaat tgaatggata
      181 taaccaataa cggtatcacg cacgttcgac tcgtgcggtt ggtattgacc gaaattaaaa
      241 aatagaaacg agaacctcct tacaccaaaa caaaatctaa cgctggttat cggtcactgg
      301 ctattattta aggcgctaat actgacatcc tgcgcctgtg tcaaaataaa aaaaaggaag
      361 agagggcttt tctccacaaa aaagccagct cacagctgac tccttagtta ataatttgac
      421 aaatctatta tatcaaaaag gagctagtta gtgagcaaag ctaaggcaat tttaaaagac
      481 ttacgcaatt tagatttata tatcgctagt ttaatcagac gtcgagataa gattgaggct
      541 tcgttgcttt ctagccctaa atggtcgtct gataaggtga atggtggtat aaaacgtaag
      601 caagatgatg tttacgtaga gttgattgca actgccaaag atattgaaaa gaagactgct
      661 gaagctataa gaaaacaaag agagcttcaa aacctgattg atagccttga aaatacagac
      721 agtcaaacga ttttgagcat ggtctatatt gataagatga ctagatggca agtgattgat
      781 gagctaaatt gcagcgaaag cacctatttc agactgttaa gagttgcaac taaggaatta
      841 aataatttga cagtaaatga cagcgattga cagtgaataa cagtgcatga cagttttaaa
      901 gtgataatat agtattatca agaaatgagg gacaggtaag aaaatacccc cctcttttaa
      961 atttagaaag gcccatccat gtctcagtta agggcagata aaaaaggtac ccaccgggta
     1021 gcatttgaca gaaataaaaa gaagttacta aaggcagcca ctgtctgtgg tatctgtggc
     1081 aagccagtgg acaagtctct taagtatcca catccattaa gcgcagcaat agatcatata
     1141 gttcctatcg caaaaggtgg tcatccatca gcgcttgaga acttacagtt aactcactgg
     1201 cagtgcaaca ggcagaagtc tgataagttg tttgctaacc aagcaagcaa cgagccaaag
     1261 acaattggca acagaaacct tcctcaaagt cgagactggt catcttttgc atttaaaaag
     1321 tgattttagg caaatatatt gatttatact aaaagctctt agaagcgaaa tggggggtat
     1381 ctccctaccc ggtcggtcgg ccgagcttca cgccgtcact gtacattttt tctcacgtta
     1441 ggatttagaa aattttggag gttgaatttc attgaaaaag aaatgcttaa tttgcaaaaa
     1501 aacttccaag caaaaaccaa tagaacttta tattgctctg aagaatgccg taagaaaggc
     1561 aatcgtgaga aacaacgtaa attgatgaaa caaaaacggg ctgaacagag gaaagaaaaa
     1621 aagaaagtcc taaatcctaa cacagatgtg acagaaaagc ctaaaaaaat acgtaattta
     1681 gcgcagcact ataaaaaact aaaaaaggaa attttggcaa atgaatctga atttggtttt
     1741 actggaataa cacttattga aggaatagat gtacatgaag aaaactttgt agatttagtc
     1801 atgcaaaaaa taaaggagca gaaatgaatt atatgggtat gggctatctt cgtaggaagt
     1861 tagctctttt taaaactgga gttgataaaa gatatcgtta ttatgccatg gatgacagag
     1921 acgacacacg aagtattgtc atgccaaata atgtgcgtga aatgtacagg tctgtgttag
     1981 aatggaccgc taaaggggtt gatagccttg cggaccgtat tattttcagg gagtttacca
     2041 acgatgattt taatgcttgg gaaattttta aagcgaataa ccctgatatc ttttttgata
     2101 cagccataca gtcagcatta attgcatctt gttgctttgt gtacatcatg ccaggagcgg
     2161 aagacggctt acctaaaatg caagttatcg aagctagtaa agcgacgggg atacttgacc
     2221 caactacatt tttattaaca gagggttatg caattttaga gtctgactca aacggtaatc
     2281 ctacgttaga ggcctatttc acagacaaag acatctggta ttatccaaaa aaagggaaac
     2341 catataatat taaaaatcca acaggtcacc ccttgcttgt acctatcatt cacagaccag
     2401 acgcagttag accatttggt cgcagtcgca ttaccaaggc tggaatgtat catcaaaagg
     2461 cagcgaagag aacgcttgag agagcagagg ttacggctga gttttactca ttcccacaaa
     2521 aatatgtttt gggaatggat ccagacgctg aaccgatgga aaagtggcgt gctacggtgt
     2581 caacgttact cgaaatctca aaagacgagg atggcgacaa gccaacagtt gggcaattca
     2641 caacagcaag catggcccct ttcatggaac acttgaaaat gtatgcttcg ctatttgctg
     2701 gtggttccgg tctcactctt gatgaccttg gttttccatc tgacaaccca tcgtctgttg
     2761 agtcaataaa agcggcacat gagaatttga gagcagcagg acgcaaagct caacgttctt
     2821 tttcttctgg atttctaaat gtggcgtata ttgctgtttg tttaagagat gaatttcctt
     2881 atcttcgtaa tcaattcatg gatacagtaa ttaaatggga gcctctcttt gaagctgatg
     2941 caaatatgct aactttagta ggggatggag ctatcaaact taatcaagct atccctggtt
     3001 tcatggatgc agatgttatc cgtgatttaa cgggagtaaa aggggctgat aaacctatac
     3061 ctgctatcac ggaggtaaca actgatggtt gatgatgtct tacctaagct actaaaatct
     3121 gttcgacaag attttgaaaa gtattttggc gaaagtgatg ttgtaaccaa ggcttttgca
     3181 gaattgcaag ctaaaaaagt gacctataaa acagtcaatg agtttgctat tgaagtcgga
     3241 agacttttat ctttggctct gacaggttct gttagctcgg ataaattacc agacggtaaa
     3301 atgtattaca atatcgctaa gcgtctctta gatgagacta tggggcggaa ttacaaattg
     3361 atttcaggtt atgctggtga tgttcagcgg attttaaatg agaatgctca gattggtcta
     3421 aaggtgcaac gtccaccgct aaatcgagac aagattaaca ggatggtaaa tcgtttggat
     3481 agcgagaata catttgatga tgtaaaatgg ctgtttggtg aaccgattgt taatttttcg
     3541 cagtccattg tggatgatac catcaaggca aatgcggatt tacaatataa aacaggtatg
     3601 acgccacagg ttgtcagaac agaaagtggc aactgttgtg aatggtgtcg tgaggttgtt
     3661 ggcacttata gttatccaaa agtccctaag gacgtatgga gaagacatca gcggtgtcga
     3721 tgtacgcttg actatgaccc gaagaatgga aaagttcaga gtgcttggag taaaatctgg
     3781 cgaaagaaag aaaaaactca agaatctata gaaagggttg aaaaatttaa ggaaagtgct
     3841 ctagtagaat ctatcaaaaa tgatatcgct aagttagata tgacaaaggt tggtccgagt
     3901 gatataattg atatagggaa acgtatcaat tatcacttta gagtttcaga acatatagga
     3961 gataaagaga aattaaaaga aattttctca aattttcgtg aaatcggtgg agaaatacct
     4021 aaaaatactt gggcaaaagg ttcatctaaa cttgttaaag accagctaca agaggctttt
     4081 caaaattatc caactgagtg ggcagctgtt ccggacggta ttggtaaaaa actaaaagct
     4141 ataaaaagga agcgcggtta cttcgatgga tatgatgaag atttagttat tgctacaaat
     4201 ggaacaagaa aaacaacacc ttaccatgag ataggacata tgattgaact ggttaatcct
     4261 gatttagtaa gattagagaa agcctgggta gataaaagaa ctgctaatga agctgaggtt
     4321 cgcttgaaag atatttttcc aagttcaaat tatggtattg gggaagttac taagaaagat
     4381 gactttatat caccatatat tggtaaatac tacagcgatg cagctgaagt tttcactatg
     4441 ggattacaag gtatttttgt tcctgaagaa cgatttgcta agtcctttga caaaaaaaca
     4501 tggaaatatg actacaagac aatcaatgac gaccctgaat ttttgaattt tattattgga
     4561 ttatttgtga aagtgtgata aatatggaac caaaattaca tcggcaactg cgtcaaaaat
     4621 atgacgacgc tgaaaaacaa tatcttgaaa agtttggaga agactcgctt gatagagtat
     4681 ttttttggga gccagacgtt tactttgatg agtggaaaaa ggttctacca gatgcaacac
     4741 tggaattaaa caaagctatt aatagcgggg tggcgattgg tccagatcca gaaaacgcaa
     4801 tatattaagc acctagagca atctggtgct tttcttatgc ccaaaaagga gaaaaatatg
     4861 aataaaatat cattaaatgt cgaaatgact tgggaaaaca aagacgaatt tcaacaaatc
     4921 atgaataaag tcaatgaaac aaaagaagct tacgaaaaag ccttagaagc tgcggaaaat
     4981 tttgtcccta aaatgtcgtg tgcaacaaag ttaaataaag gagaaggcga tgaataagcg
     5041 aatcaaaaag aaacgtaaac tcgaacgggc tattgtgatg ctggttgcgg aaaatgctat
     5101 gcaagcagaa gcaattaaaa atcaaaacaa agaaatcatg gagctaaaat caatcgttca
     5161 acgaaacgct ctggcaacaa acgaagagtt tgcgtctgtt aaagctgcta ctttagataa
     5221 ccaatcagct attgcagata ttgcaattga cgttaactat attaagaaaa attacaagcg
     5281 aaaatgggga aaataaattt taaacgaatt cgaccccttt agaaatcaaa gtcgtagcaa
     5341 tacggctttt tattgtgccc tgtcgcatgg ctaaaaacta ggcagtacga ttgaaaggaa
     5401 taagtatggt tactaagacg aaaacaaagc ttggcaatca gcgacctact caatcggtaa
     5461 atttacattt tgctaaatct ctagcgcatg aagctattaa ttactacaaa aaaacagggc
     5521 taagctgcta tccatggcaa gtaaatatgc ttatcccaat tatggccata gatgaaaatg
     5581 gtctgtgggt tcatcaaaag tatgggtatg ctatcccacg gcgtaacggt aagacggaag
     5641 tagtctatat tgttgagctg tgggctttgc ataaaggttt aaaaatcttg catacagctc
     5701 atcgaattag cacatctcat gcatcattcg aaaaggtaaa aaaatacctt gagatgtcag
     5761 gttatgttga tggagaagac tttatatcaa ataaagccaa gggtcaagag cgtatagagt
     5821 tcaaagccag cggcgctgtt atccagttcc gaactaggac atcaaacggt ggacttggtg
     5881 agggatttga cttacttatc attgacgagg cacaagaata cacatctgag caagaatcag
     5941 cattgaagta cacagttact gatagtgata atccaatgac tattatgtgt ggaacgccgc
     6001 caacgatggt atctactggt acagtatttg aagcatatcg gaaagattgc ttaaaaggca
     6061 ataagcgtta ttctggttgg gctgaatggt cagttcctga gatggttaag attaacgatg
     6121 tatcttcctg gtatatttcc aacccatcta tgggattcca cctcaacgag aggaaaatcg
     6181 aagctgaatt aggtgaagat gagattgatc acaacatcca acgcttaggt tattggccat
     6241 cctttaacca aaaatcagtt atatccgaaa aagaatgggc aaaactcaaa gttgagcaag
     6301 tgccagaact caaaagcaag ctttttgtcg gtatcaagtt tggtcaagat ggcaacaacg
     6361 tatcactatc aattgcagca agaacatcag aaaataaggt atttgttgag actattgact
     6421 gtttatcggt cagaaatgga actcaatgga ttattaattt tttgaaatcg gctgatatcg
     6481 ctaaggttgt cattgatggt gcaagtggtc aagaattact tgctcaggag atgaaagatc
     6541 aaggtctaaa gaaaccagaa ttgcctaaag ttgctgaaat tatcacagct aacatgatgt
     6601 gggagcaggg gatcatgcag gaaaccattt gtcatagtga tcagccatct ttgacagcag
     6661 tagtcacaaa ctgtgaaaag aggcaaattg gctctaatgg tggttttggg tataaatcgc
     6721 tttatgatga tagagacatt agcttaatgg acagtgcatt gcttgcgcac tggatttgtt
     6781 acacaacgaa gccaaaaaga aagcaaagaa ccagctgtta aaaaacgaca tccgaaaggg
     6841 tgttttttta ctgctaaaaa atctaccgaa ctgccgggaa agcaggagaa aggacgttaa
     6901 tatgtcagaa tttaaagtta ttgaaacaca agaagagttg gacacgattg tgaaagctcg
     6961 cattgctcga gaacgtgaga aatatcaaga ttacgaccaa ctgaaaactc gtgttgaaga
     7021 actagaaacc gaaaacagca gcttacaaac tgctttgaat gatgctaaat caaacactga
     7081 tagctatacg gaagagatta gcaccctgaa gaatcaaatt gccgattatg aggcggcaaa
     7141 tttacggaca aaggtagcgt tacagtatgg cttaccgatt gatttagctg atcgtttgca
     7201 aggagatgat gaagatggac tcaaagtaga tgcagaacgc ttagcatcct ttattaagcc
     7261 atcccaacca caaccgccag caaaatcaaa cgaaccaaat atcgatagta atgcagacgc
     7321 aaattacaga gcgttagtgc aaggattaag tacagaagat tagaagatta attaaaggag
     7381 aaaaaatatg ggaacagaaa catcaaaagc gagcttattt gacaaacatt tagtatcaga
     7441 tcttatcaat aaagttaagg ggcatagctc actagctaaa ctatctagcc aaaaacctat
     7501 tccgtttaac ggatctaaag aatttacgtt tacattagat tctgatattg atgtggttgc
     7561 tgaaaacggt aaaaaaacac acggtggctt atcgctagag cctgttacta tcgtaccaat
     7621 caaggttgag tatggtgctc gtctttctga tgaattttta tatgcaacag aagaggaaaa
     7681 gattgatatt ttgaaagctt ttaacgaagg gtttgcgaaa aaactcgctc gtggtattga
     7741 cctaatggcg atgcatggta tcaatccacg tacaaaaaaa gcgtctgacg ttatcggcac
     7801 gaatcacttt gatagtaaag tgacgcaagt ggttaaattt acagagagcg aagacgctga
     7861 tgcaaatatc gaagcagctg ttaatctaat tcaggggtct gaaggtgtag tgacagggct
     7921 tgcgatggat acagagtttt caacagcact ggcgaaagtt accaatgggg agatggggcc
     7981 taaaatgtac cctgaacttg cttggggagc gaatccagat agcatcaatg gcttgaaatc
     8041 gtccgtcaat actactgttg gtgctggagc tgatgaagca gaatccaaag atttagtaat
     8101 tattggcgat tttgaaagca tgtttaagtg gggttatgca aaacaaattc caatggaaat
     8161 cattaaatac ggtgatcctg ataattcggg gaaagacctt aaagggtata accaaattta
     8221 cttacgtgct gaggcgtata tcggctgggg tattttggac gctaagagct ttgctcgtgt
     8281 cactaaagga gaagtataat gttatacgtt aacagtaaga ctggagctag tttttatagc
     8341 tccgtccctg tttgtggtgg agattggact ttggttgaac agcagacagg cgaaaaagca
     8401 aaaacagtag cagaaattaa acgtcagtta gatgctgctg ggataaaata tagctctaaa
     8461 gctaagaaac ctgaattaga ggcactcttg cctggttagg aggtaaaagt gggcaatttc
     8521 gcaacaacag atgacgtcat tttgttatgg cgtcccttat ctgttgacga attgaaacgt
     8581 gcaaatgcac tcttgaaagt cgtatcagat acattaagaa tggaagctga caaagttggc
     8641 aaagacttag ataaaacgat ggttgataag ccttattttg ttaatgttat taaatcggtt
     8701 acggttgaca ttgtagcgag aacgctgatg acatctactc gaggcgaacc aatggcccaa
     8761 gagagccaat cagctttagg atacacatgg tcaggcactt atttagttcc aggcggcggt
     8821 ctttttatta aggacagtga gttgaagcgt cttggactaa aaaaacaacg atatggagga
     8881 attgaacttt atggcgaaat tgaaagggat aacagttgct ttagccgata agtcgattag
     8941 cggaaaagac ccttttggaa atcctgtaac agttgatttt gatatcaaaa ttgagaatgt
     9001 gcttgttgca ccagcaacta ccgaagatat cactaatcag ttatctttga ccggaaaaaa
     9061 ggttgaatat gtcttggcta taccaaaagg agacgaacac gattgggaaa ataaggaagt
     9121 tagatttttc ggcaaaaaat ggcgcacagt aggaattcca ctagaaggta ttgaagagct
     9181 tataccacta gactggaata agaaagttat ggtggaaaga tatgagtaag tttaaattca
     9241 agcttaatag agctggtgtt gccgaattga tgaaatcatc agaaatgcag caggtattaa
     9301 ccactaaggc cacagccatc agagaacgtt gtggtgatgg ttatgtccaa gatatccatg
     9361 ttgggaaaaa tagggctaat gctatggtca gtactaaaac cataaaggcc aagaaagata
     9421 actcaaaaaa taacacactg ttgaaggcgg tgcgatgatt gattgaagta attatcaaaa
     9481 aatatttaga cgagcactta gatgtgccgt ctttttttga acatcaagaa gatgaacctg
     9541 cacgattcat catcttagaa aagactagtg gggctaagca aaatcatttg ctaagttcca
     9601 cgtttgcttt tcaaagttat gccgaatcgt tgtatgaggc ggctttactt aatgacaaag
     9661 taaagcaagt aattgagcag cttgatgtct tgccacaagt ttctggtgta catcttaacg
     9721 ctgactacaa ttttacagat acagcaacta agcgctatcg ttatcaagct gtatttgata
     9781 ttaatcatta ttaaaggaga tattgatgaa taagaatgat actaaaaatg taacatctgc
     9841 aaagcccaag accggtgggg cgatttattc ggcaccgctt ggtactgaat tgcccaagga
     9901 tgcaaaatca gaattaaata ctaaatttaa gaatttgggt tatgtatccg aggatggtgt
     9961 ggtcaatgaa gatacacgtt catccgaaaa tattaaagcg tggggtggag atattgtcgg
    10021 ggctgtacag acagaaaaag aggataaatt tacttataag ctgattgagt cactaaatgt
    10081 agaagtctta aaagaagttt atggcgctgt caatgttact ggagatttaa gtgggggaat
    10141 ccagattaaa tcaaattcaa aagagttaga agctcacgta attgttgttg acatgatcat
    10201 gaatggcggc attcttaaac gaattgtctt accaaatgca aaagtcgatg aggtaggtga
    10261 aattaagtat gttgacggcg aagtcgttgg ttatgaaaca acactaaaat gtttcccgga
    10321 caaggatggt gatacacacc gtgagtatat tgtaaaacct ggagaagcta ataaaaaaga
    10381 aaacagcttt gaaatgtaaa ggggagtaaa tggaaatctt aaaaggaaaa acaacatcag
    10441 gatttgaata cgaaattcct aaaaaacgat taaaaaactt tgaacttgtt gaagctattg
    10501 cagaagagga aactgatcca acagcggtag ttaaaatcgt taatttgtta cttggtgatg
    10561 ctgctaagtc tctaaaagaa catgtacgag atgcagaagg tatcgtagac gttgaagcta
    10621 tcggagtaga aatcaaagaa atttttgaaa gtcaaaaaga tttaaaaaac tagcaatcct
    10681 cgctcagatg atagcaaaag atgatgatgc attgacttgt gatttagctg aaacatacgg
    10741 catatatgat tacagacagc tacctgctta tcaggtggct gtttttgctg tcggtttgag
    10801 atctaactct aggataaaaa tggcattatc tggagagact gaggctttgg atactgtttt
    10861 gttagctggt atttatgata atactaattt gctgttttgg tctaaaacta aggatggtca
    10921 atctggtcaa aacaaaccta aatccgtggt ggaagctata tctggatcta aatcacaaaa
    10981 agctaatgat gtcatttctt ttgtgtctgg cgaggatttt gaaaatgcac gtaaacaatt
    11041 actaggaggt gatggctaat ggcaacagaa cttggtcaag cgtatgtgca aattatgcca
    11101 tccgctcgtg gaataagtgg agcaatctcg aagcaacttg atcccgaagc aaggtcggct
    11161 ggattgagcg ctggttcgct cattggtggt aatctcgtta aaatgattgg tggtgccatt
    11221 gcagctgctg gaatcggtaa gatgatttcg tctgccttgt ccgctggtgc tgatttgcag
    11281 caatcttttg gtggtattga cacattgtat aagggcgctg agactgctgt caaagggttt
    11341 gctaaagagg catacaaagc tggaatatca gcaaatactt atgcagagca agcagtttca
    11401 atgggtgcat ctctaaagca atcacttgga ggtgatgctg tcgcggctgc caaggctgct
    11461 aacatggcaa tcatggatat ggccgacaac tcggctaaga tgggtactga tatcacatca
    11521 atccaaatgg cttaccaggg atttgctaag caaaactata caatgcttga taacctaaga
    11581 cttgggtacg gcggcacaaa agaagagatg aagcgtcttt tatcagacgc tgaaaagtta
    11641 cctgccgcta tgggcaagaa gtttgatttg agtaattatg ctgatgtggt tgaggctata
    11701 cacttggtac aggataacat gggtatcgct ggagttgctg ctgaagaagc aaaaactaca
    11761 ttttcaggct cactagctgc tatgaagtcc tcttttacaa atgtaatggc aggtttatca
    11821 ctaggagatg atatcagacc ggctttacga ggactggctg agacaacttc taatttctta
    11881 tttggtaact ttattccgat ggtggcaaat atctttaaag gattaccatc ggcaattggt
    11941 acttttattg gagccgcagc tcctattatc acaagtcaat tccaaggtct aatgagtagc
    12001 cttggaatta gtattgattt aagtcctatt actgctaaat ttgcacagat tggccaaaat
    12061 ttacaacctg tttttaacgg tttaaaaacg gcttttagtc agttgccatc attttttact
    12121 agcattggta gtgcagttgc accagtaata gacactatta ttagcggatt agccagatta
    12181 gatttcagtg gttttgaggc tttaatttca gcaattttac cagccctaca ggcaggtttt
    12241 tctaacttcg ctgcgattgt tggaccagct atctcaggag ttgttgactc gtttgttggc
    12301 atgtggaatg cagcacaacc tttaatttcg atactaagtg atgccttgat gccagtattt
    12361 caaattttag ggtctttctt aggtggtgtt gtaaaaggtg cgcttatggg agttagcttt
    12421 gcctttgatg cagttaaagt ggctatacaa ctagtcacac caattattga cttgctggtt
    12481 caaggtctta attttgtaca acctgttctc agtgtcattg cagaatggat tggagttgct
    12541 atcggtatgt ttggtaattt aggcacagcc ggccaaggct tgagcgcttt tattaagagc
    12601 gcttggacta acattcagac tgcaatttca actgctggaa caatcatatc tacggttatt
    12661 gactacataa aattagcgtt cagtggtgcc ggttctgcag ttggcgtcct aaaaaacatt
    12721 ttctcacttg cttggatggc aatgggagac gcaataaatg tggctaaggg tatcataagc
    12781 tctgttataa atggcataaa atcagccttc agtagtttta gtagcttagt gtctagtgtt
    12841 ggttcagcag taaatggagt tatcgattca atctcaagca caattagagg tttggcaaac
    12901 attgatattt ctggagctgg tgccgcaatt atgaatggtt tcttaaacgg tttgaaatca
    12961 gcttggggag ccgtcaaaag ttttgtgagc ggtatcgcca actggattgc agaacataaa
    13021 ggacctatct cttatgatag agttctacta aaacctgctg gtaaagcaat tatgggtgga
    13081 cttaatacaa gcttgattga cggctttaaa gaggttaaat caaatgtctc tggcatggct
    13141 gacgaccttg caagcaccat gacaggtaaa agtctatctc tcggtatcga tgctaaacca
    13201 agcgtcacag ctgatgactt actatcaagc aatattagta ctaaaactac agtcggttct
    13261 gctacaagtg acttgtcatt attctttgtt aaggtgcttg ctctgttgca agatatcctt
    13321 gataaaaata cggatgtcta tctagacaaa gaaaaagtca gcgctatttt atacgaagaa
    13381 tttgccaaaa ttatggctag agaggggatt gtatgatacc taaagttatt attgatgatt
    13441 ttgacacatc ttcaatccct aattgtgttt tgaccggtta cgatgtgggg gatattctat
    13501 cccctagttt tgtcgaaaat gaagcttatg gcatgaatgg aactagtagg gaattggagt
    13561 catataatga atctaaacca accataatgt ggcatttgag tacttttgat gatgcagtta
    13621 atctaattaa tcatttagac ggccttagta aaaaaatcga attttggcac atacctaact
    13681 ctatttatta ctatgattgc ttatctgtca aaatcaatgc ggtaaccatg tcatcatggc
    13741 gtgtgactct caaacttgct ctttatccat ttagatacgc aaaaggtgtc tcagatgtag
    13801 taattgcagg caacggaaac attaacaatg caggaaatgt tttcagcgaa cctaagatag
    13861 ttgttgaggg tactggtaaa ggaacgctaa ccattggcaa acaggtcatg gaattaaatt
    13921 tgtcaggtaa agcaacgatt gagtgcaaac atggccaaca atgcgtctat gatgctgaag
    13981 gtaatgtgaa aaactcaatc cgaataagag gaagtttttt tgaaatacaa cctggcacac
    14041 aaggtattgc cgtcagtgga ggcattacta gaacaataat tagtccaagg tggaggtata
    14101 aggtttgata tcgattaaag atgataatac ccctcttgta gcagcctttg aagatgagat
    14161 tacacaggag gccaatagtg attacaaact aaattttaag tatcctgcta aacacgagta
    14221 tcgcccttta ataaaaaaag gaataatctt agaagctgat gatctgcatg gttctcagct
    14281 ttttaggatt tttgaaatta ctaagcggca tggctatatt aacgtttacg ctaatcaggt
    14341 cgctgatgac aatacaatgc acgactttgt aaagcctaaa gttaccactc aacaagcttt
    14401 tggactagct cagcaggtca aagagcttga tttacagcta aaagacccta agtcagattt
    14461 gtggggcaaa atcaagttca ataataaggc aatgctagtc gagtacgcca acaaagagat
    14521 gtcaagtgcc attgcgcaat cagctgagca gatattgtta caagtcaagt ctattgatga
    14581 tgaacgatat tccaaatttg agcaaactct gaatggtatc aaacaaactg tcaaaagtga
    14641 gtcagttgaa tccgcacgta ctcagctagc atcaatgttt gatagtcgta ttagtggact
    14701 tgatggcaaa tacagtcgtt tgagccaaac aattgatagt cttagcagtc gtcttgatga
    14761 tggtgttggt aactactcaa cgctatctca aaaggtaagt ggcattgatt tacgagttag
    14821 taatgcagct aatgatgttt ctcgattgtc tcagacagca caaggattgc agtcacaaat
    14881 cacaaatgca aaccaaaatt acagcagttt gtctcagact gtacagggac tacaaacaac
    14941 tgtacgtgat aatcaatcaa atgctacaag tcggattaat cagttaagta acctgatcag
    15001 cacaaaggta tcaaaaggtg atgttgagac aactattgct cagagttacg acaagatagc
    15061 cttcgcaatc agggataaac tcccagcaag caaaatgtct ggcagtgaga ttatctcggc
    15121 aatcaatctt gataggtctg gggttaaaat cactggaaaa aatatcactc ttgatggtaa
    15181 cagctacatc agcaacgctg tcatcaaaga tgctcatatt gctaacatgg atgccggtaa
    15241 gattaatact ggttatctta atgctaatag gattgcaacc gaggccatta ctggtgagaa
    15301 aattaagatg gactatgcct tttttaataa actcactgct aacgagggat attttaggac
    15361 gttgtttgcc aaagacatct ttgcaacatc agtccaatct gtaacactat cagctagcaa
    15421 aattactgga ggtgtattag ccgctacaaa tggggcaagt cagtgggacc taaataatgc
    15481 caatatgacc tttaatcgag atgccacaat taattttaat agcaaaaaca atgccttagt
    15541 acgtaaagat ggcacacata ctgcttttgt acattttagt aatgccacac caaaaggcta
    15601 tagaggctca gcgttatatg cctctatcgg cattacctca tcaggagatg gcatcgacag
    15661 cgcttcttcc ggtcgttttg cagggctaag gtcatttagg tacgctacgg gatataacca
    15721 tactgctgca gtcgaccaaa ccgagctata cggtgataat gtcttgattg cagatgactt
    15781 tagcatcaat cgaggattta aatttagacc acacaaaatg gaaaaagtgc tcgacatgaa
    15841 cgacttgtat gcggctgtag tagccttagg ccgctgttgg gggcacttgg ctaacgtcgg
    15901 ctggaatact gctcatagca attttacaag tgctgtgagt agggaattga ataactacat
    15961 cactaaaatt taacaggaga taatatgcaa ttaactatta aaaacaaaga tttaaacaca
    16021 ctatatcgtg tactagacaa aatcaaagtc acgaacatgc gagcaaaccg cggacgtgct
    16081 aagctactcg caaaagtagt agataaattc aaagagtacg ccaaggatga gggtgacctt
    16141 attgatctgt atgctcaaaa agacaaagat ggcaagtttg tcattgatga gcacaaaaac
    16201 atcaagctag cagaccccgc taaactcgac gagttcaacg gcctactcaa cgagctagct
    16261 gatgaagaaa ttgtgattaa agggggtgag tactccaagc gatttattga ctttttaaac
    16321 tttttagaag agtgtgaaga tgaatttaca tcatctgaaa tcattcttat cgacaacatt
    16381 ttggaacaat ttgaagaaag taaaaaagga gaaaaaccat gagaaattgg aaagtgacag
    16441 gaaaataccc acaatttgac agcacaggag cagtcgcaag tacacacgtc attatcgctg
    16501 cagaagacgg cgctgttatc ccgcaacttg ttaaaaaaga tttaacaggc acaaatgata
    16561 aagacattat cacagcagtg ctcgaagagt ttaaaaaatc tgaatacgtt gaaattgcaa
    16621 tgggcgaagc cgtgcaaaaa gtagacgacc ttgaaaaaat ctcacaggaa accgctaaga
    16681 ctgccaaaac tgctcaaaca gctgctggac tagctaaggt gtccgcagag cgtacacagc
    16741 gaatgattaa cttgcaaacc atccacatgt taacgagcgg cggcaagatt gatcctgaca
    16801 tttataaagg catgcttgag ctaatcgagc cagccaaaaa aggtgagtat caagcctatg
    16861 atgtctttac ggtggtcgac agtactaaag aggaagacgg tgaagcaggc gaagggaacc
    16921 tagtctttgt acacgttaat gaagcatttg agtatgacaa acaaacctta gaggagctag
    16981 agtcagaagc caaagtaaca gttatcaagt acgctgattt ggttaaacaa gattagaggt
    17041 gttttatggc aacagagttg atatttggcg tcggtggctt tattttagct atcgtcacga
    17101 cttacaatat ttttaatgca aaatccatca agcatgcgac agatattact ttgttgcaat
    17161 ctgaggtaga gcatttaaaa attgttacac gccaaaatgc tcggcggctt gaagagcacg
    17221 ctgagcaaaa taaaacgttg attacaatga cagagcaaat taaaaattta acagatgatg
    17281 ttaaagattt aaaagatatg atgagaggag aaatcaaatg atcaatttaa aattacgact
    17341 acaaaacaaa gtaaccttga tggctatttt gggagctatc tttttgttag cgcaacaatt
    17401 aggtattaaa ctaccatcaa acatcgcgga tattgccaac acagctgtaa cgcttttggt
    17461 attgctcggt gttgtcacag atccaaccac aaaaggcctg tcagacagtg agcaagcatt
    17521 gacttaccac gaaccaaaac aataggagga gccatgcgag caatcacacg attagcatta
    17581 gttatagcaa tcgcaatact gtatgtgccg atatctgttg ttgctctgat cttttatcca
    17641 tttttagata aggaggacag atgacctttt tagataaaat taaacaaggc tgtttagatg
    17701 gctggacaac gtacaaaatc ttgccatcct tgaccgcagc gcagacaatc ttagaaagcg
    17761 ggtggggcaa acatgcccca cacaacgctc tgtttggtat taaggcagat agctcttgga
    17821 ctggtaaatc atttgatacc aaaacccaag aggaatatca agcaggtgtt gtcacggata
    17881 ttgtggaccg atttagggcg tatgatagtt ggactgacag cattattgat cacggtaaat
    17941 ttttaaacga taatccacgg tatcaggctg tgattggtga gactgattac aaaaaagctt
    18001 gtcacgctat aaaagatgca ggttatgcca cagctagcgg ttatgcagag ttgcttatcc
    18061 aactaatcga ggaaaatgac cttcaggaat gggatgaaga ggctattgtc agaaaggaga
    18121 agcaaatgat tagttctcaa tgtcgagaag tcattgaatt ttttattaac ttggtaaatg
    18181 ctggggttgg cgtggataaa gatggttttg cgggctggca atgcacagat gtgccttgtt
    18241 atgcagcaaa gcactggttt ggtgttgatc tttggggaaa tgcgattgac ttactagata
    18301 gcgctgctgc cgtaggttgg gaagtccacc gcatgccgac agatgcaaat ccacagactg
    18361 gagcattttt tgtccaatca gtgccatatc atcaatttgg tcacacagga gttgtcattg
    18421 aggatagcga cggttatacc atgcgaacta tcgagcaaaa cattgatggc aatcctgatg
    18481 ctttgtatgt cggtgcacca gctcgtttta atactcgtga ttttaccggc gtgacaggat
    18541 ggttttaccc tccttaccaa ggagatattg tcacgcaacc agtcagcacc gagccacaaa
    18601 cgtctgacac tatcgtagag acagcaaaaa caggtacttt tacgcttgat gtcgcagaaa
    18661 tcaatatcag acgttggcca agtttaacta gcgaggtagt aggtagctat aaacaaggtg
    18721 atactatcag ctttgatagt gagggttacg ccaatggtta ctactggatt agctacgttg
    18781 gcggctcggg tatgcgaaac tacacggcta tcgggataac tgacaaagac ggtaacatta
    18841 tcagcctttg gggcaaatta aattaggaga taaagctcct ttagataaga caaaaccgct
    18901 ctcttaatcg agggcggttt tttgtgttta agtaattatt tttttaattt ttttctttca
    18961 ttttcaattt ctatttctcg ttttattaac ctatgaagga ggaatggaag tagtgctaaa
    19021 atagaacaac ctgtaagaaa tattgcactt actttgtcag aacgattagt tgataaaatg
    19081 gtcgataaat tatttgatag actatttgtc aaaatagaag caaaatataa tgaattgaaa
    19141 gccagtaaaa tagtattctt ccatctatct acagttagct ttttatattc ttcctgcaaa
    19201 ccatcatatg ttttaataag ttcaaaagcg gaaacaatta tcccattaaa aaaaatactc
    19261 attaaaagaa cgataagttc attgtcataa ttaacaatat aaattggtat cacagctaaa
    19321 gccattccaa caataacact aagtaacgat gcttttttgg accttttcat aaaatcaccc
    19381 ttttttttca atctactata catattactc gattcaatca tcctcaacaa aggccgttaa
    19441 aacagactaa tttctcaaag tcctatttaa gggactttct aaatagacgt tgttttttta
    19501 aaaaatagca cagatagtag cctatactaa tttttcttca aaat
//
LOCUS       DAXWIQ010000035        17794 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000035 DAXWIQ010000000
VERSION     DAXWIQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17794
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.035"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            124..345
                     /locus_tag="ACGEUI_001287"
     CDS             124..345
                     /locus_tag="ACGEUI_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8166124.1"
                     /translation="MTKSTLKELRTTKKMTQQELSHLTGISVRTIARYEKDTKKLRRA
                     KYEKLKGIAQALAVSVDDIFLGIDSEFMN"
     gene            complement(425..2707)
                     /locus_tag="ACGEUI_001288"
     CDS             complement(425..2707)
                     /locus_tag="ACGEUI_001288"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HFF8166125.1"
                     /translation="MRYNQFSYIPTSLERAAEELKELGFDLDLQKTAKASLESFLRKL
                     FFHYPDSDYPLSHLIAKNDMDALSFFQSEQELSKEVFDLLALQVLGFIPGVDFTEADA
                     FLDKLAFPIHFDETEIIKHIHHLLATRCKSGMTLIDDLVSQGMLTMDNDYHFFNGKSL
                     ATFDTSQLIREVVYVEAPLDTDQDGQLDLIKVNIIRPPSQKPLPTLMTPSPYHQGINE
                     VANDKKLYRMEKELVVKKRRQITVEDRDFIPLETQPCKLPIGQNLESFSYINSYSLND
                     YFLARGFANIYVSGVGTAGSTGFMTSGNYAQIESFKAVIDWLNGRATAYTSHSKTHQV
                     RADWANGLVCTTGKSYLGTMSTGLATTGVDGLAMIIAESAISSWYNYYRENGLVCSPG
                     GYPGEDLDVLTELTYSRNLLAGDYLRHNDRYQELLNQQSQALDRQSGDYNQFWHDRNY
                     LKNAHQIKCDVVYTHGLQDWNVKPRQVYEIFNALPSTINKHLFLHQGEHVYMHNWQSI
                     DFRESMNALLCQKLLGLANDFSLPEMIWQDNTCPQNWQERKVFGTSTIKELDLGQELL
                     LIDNHYGEDEFKAYGKDFRAFKAALFKGKANQALIDILLEEDLPINGEIVLQLKVKSS
                     ENKGLLSAQILDYGKKKRLGDLPIALTQSSIDNGQNFSREPLKELPFREDSYRVISKG
                     FMNLQNRNNLSSIETIPNNKWMTVRLPLQPTIYHLEKGDTLRVILYTTDFEHTVRDNS
                     NYALTIDLSQSQLIVPIASN"
     gene            complement(2729..3388)
                     /locus_tag="ACGEUI_001289"
     CDS             complement(2729..3388)
                     /locus_tag="ACGEUI_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8166126.1"
                     /translation="MNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILKDGIV
                     KQSVLSKYGMEFNLRYVTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSAFL
                     KDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIGAISTQIYDLMTLFGEELPNG
                     QILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIV
                     F"
     gene            3554..4750
                     /locus_tag="ACGEUI_001290"
     CDS             3554..4750
                     /locus_tag="ACGEUI_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8166127.1"
                     /translation="MEEKLFNKHFVAITVINFIVYMVYYLFTVIIAFVATRELGAQTS
                     QAGLATGIYILGTLLARLIFGKQLEVFGRRLVLRGGAIFYLLTTLAYFYMPTISMMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPARKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HIDFRMIIVLCSVLIGCVVVGAFAFPVKNMSLNAEQLAKTKSWTVDSFIEKKALFITA
                     IAFLMGIAYASVLGFQKLYTSEIHLTTVGAYFFVVYALIITITRPAMGRLMDAKGDKW
                     VLYPSYLFLAMGLFLLGSVSSGGSYLLSGALIGFGYGTFMSCGQAASIQGVDEHRFNT
                     AMSTYMIGLDLGLGAGPYLLGLIKDLALGSGVASFRHLFWLAAVIPLICTLLYLLKTK
                     TRQVVS"
     gene            4775..5082
                     /locus_tag="ACGEUI_001291"
                     /pseudo
     CDS             4775..5082
                     /locus_tag="ACGEUI_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645875.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5252..6100
                     /gene="gla"
                     /locus_tag="ACGEUI_001292"
     CDS             5252..6100
                     /gene="gla"
                     /locus_tag="ACGEUI_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaglyceroporin Gla"
                     /protein_id="HFF8166128.1"
                     /translation="MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLV
                     IAFGYGLGVMMPALMFGNVSGNHINPAFTVGLAVSGLFPWAHVLQYVVAQLLGAIFGQ
                     LVVVMVYKPYFMKTENPNHVLGSFSTISSLDNGQKDSHKASYINGFLNEFVGSFVLFF
                     GALALTKNYFGVELVGKLIEAGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSL
                     GGPTGPALNPARDFGPRLLHHFLPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAF
                     KYLYIR"
     gene            complement(6436..7332)
                     /locus_tag="ACGEUI_001293"
     CDS             complement(6436..7332)
                     /locus_tag="ACGEUI_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFF8166129.1"
                     /translation="MNEMLILRLIQALLVSAMLFIFFMLVKHLKKNKINPFKRFWTGF
                     WIGLLTDALDTLGIGSFATTTTCFKLTKLVTDDRQLPGTMTVAHVLPVLIQSLCFIFV
                     VKVEVLTLLAMAAAAFIGAYFGTHITKNWHAPTVQRILGSLLIIAAIIMIIRIIYHPG
                     EHLSDTIHGLHGIWLFVGIGFNFIVGVLMTMGLGNYAPELIFFSLMGLSPTVAMPVMM
                     LDAAMIMTASSSQFIKANRVSWDGFAGIVSGGIIGVLLAVFFLTNLDINSLKLLVIAI
                     VFFTGGMLIRSSFTSHKQQTNR"
     gene            7630..8382
                     /locus_tag="ACGEUI_001294"
     CDS             7630..8382
                     /locus_tag="ACGEUI_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA N-terminal domain-containing protein"
                     /protein_id="HFF8166130.1"
                     /translation="MTLMENITFKTPVLRVNDRDLNIAFYQNNLGLRLVSEENAIAIF
                     SSWGEGQECFVIEESPSVRTRAVEGPKKVNTIVIKTNQPKEIEQLLAHGAHYDALFKG
                     QNGYAFETISPEGDRFLLHAEQDIKHLQGTDLPSLEKDATFKGLTQFKFDIIVLNVIS
                     EERSKAFYRDLFSDQLPITMDFIQEEGPDLAIDPHIAWDLEILEFQVSKDYDMKVLKA
                     TLEEDGHKVYIDKKHKVLVLSDPSQIEVWFTK"
     gene            8367..9317
                     /locus_tag="ACGEUI_001295"
     CDS             8367..9317
                     /locus_tag="ACGEUI_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFF8166131.1"
                     /translation="MVYEMTLAVIKCIENHLHKKVYKGASLALFQSGRWQEYHIGTID
                     GRRPVDANLVYDLASVSKVVGVATICNILLNNGTLALDDPLKVYYPSIADATVTIRQL
                     LTHTSGLDPYIPNRDVLNAQQLRKALNHLTQKENKNFYYTDVNFLLLGFMLEELFSES
                     LDQIFDKTIFTPFGMYHTSFGPRPEAVPTLKGVSDGEVHDPKAKILKKHSGSAGLFST
                     LADLESFSNHYLNDPFSDCLWRNYSQQTIERSLGWNLDGDWISHTGYTGPFLMLNKKE
                     QTAAIFLTNRTYDEDDKSKWLKERQLLYNALKHDLTTPVS"
     gene            complement(9495..11822)
                     /gene="pflB"
                     /locus_tag="ACGEUI_001296"
     CDS             complement(9495..11822)
                     /gene="pflB"
                     /locus_tag="ACGEUI_001296"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFF8166132.1"
                     /translation="MATVKTNTDVFEKAWEGFKGTDWKEKASVSRFVQANYTPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEATRFPYDTRPTSIADIPAGFIDKENELIYGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEV
                     NLQYQALGEVVKLGDLYGVDVRRPAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFGRTKAYDALYSGDPTFITTSMA
                     GMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDQLPETFRRYCMKMSHKHSSI
                     QYEGVTTMAKEGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGY
                     DDVHRDYKVFNVVEPITSEVLEYDEVMANFEKSLDWLTDTYVDALNIIHYMTDKYNYE
                     AVQMAFLPTHQRANMGFGICGFANTVDTLSAIKYATVKTIRDENGYIYDYEVTGDFPR
                     YGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEASVSLLTITSNVAYSKQTGNSPVHR
                     GVFLNEDGTVNTSQVEFFSPGANPSNKAKGGWLQNLNSLAKLEFSHANDGISLTTQVS
                     PRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLNDVYDKIMNGEDVIVRISGYC
                     VNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK"
     gene            12031..13125
                     /gene="dinB"
                     /locus_tag="ACGEUI_001297"
     CDS             12031..13125
                     /gene="dinB"
                     /locus_tag="ACGEUI_001297"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFF8166133.1"
                     /translation="MLIFPLINDTSRKIIHIDMDAFFAAVEERDNPALKGKPVVIGKD
                     PRETGGRGVVSTCNYEARKYGIHSAMSSKEAYERCPKAIFISGNYEKYRTVGDQIRRI
                     FKRYTDVVEPMSIDEAYLDVTDNKLGIKSAVKIAKLIQHDIWKEVGLTCSAGVSYNKF
                     LAKLASDFEKPHGLTLVLKEDALCFLAKLPIEKFHGVGKKSVKKLHDMGIYTGQDLLA
                     VPEMTLIDHFGRFGFDLYRKARGISNSPVKSDRIRKSIGSERTYAKLLYQETDIKAEI
                     SKNVKRVAALLQDHKKLGKTIVLKVRYADFTTLTKRVTLPELTRNAAQIEQVAGDIFD
                     SLSENPAGIRLLGVTMTNLEDKVADISLDL"
     gene            complement(13218..13700)
                     /locus_tag="ACGEUI_001298"
     CDS             complement(13218..13700)
                     /locus_tag="ACGEUI_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166134.1"
                     /translation="MISYEKVRQALKTSTIAIIILNGLGVVLSLMGFAGIFYLQSQLK
                     NEAFRAQLTTEQLAQLQSSMTPFMIFLSVLNVLAIIAIIVFCAQNLSKLKQGLTVSYI
                     PYILGLILSVIGLVNQFTTTMSMVGTILILIQAALYGFAFYKAKTLNEKGDDTDQAML
                     "
     gene            complement(13791..16244)
                     /locus_tag="ACGEUI_001299"
     CDS             complement(13791..16244)
                     /locus_tag="ACGEUI_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFF8166135.1"
                     /translation="MEYVFTGTVDRIIFENQANFFKILLLAIEDTDSDIDDFEIIITG
                     TMADIIEGDDYTFWGELTQHPKYGQQLKLSRYQKIKPSSSGLVNYFSSDHFKGIGKKT
                     AEKIIALYGHNTIDHILEDPSKLETISGLSKANRQAFVAKLKLNYGTEQLIAGLVELG
                     LSNRFALQAFEKYKEEALDLVKENPYQLVEDLQGFGFKMADALAENLGIESDSPKRFR
                     AALLHCLLEESINRGDTYVQARQLLDFAITLLEDARQVECDPAAVAEQLSELIIEGKI
                     KNSDTKLFDASLYFAEEGIANNISRLLDTPLSQSFSHDTIQTTIQAVQKDFAITYDQV
                     QQEAITKALTSKVFLLTGGPGTGKTTVIRGILQAYANLHQIDLDKKDLPILLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDNDYQAMEDYLDCDLLIVDEFSMVDTWLANQLLG
                     AINSTTQVIIVGDSDQLPSVGPGQVLSDLLKVNSLPQIALQKIFRQSQESTIVNLADQ
                     MRRGILAADFRDKKADRSYFEAQAAFIPDMIQKIVLSAIKSGIPAEEIQILAPMYKGQ
                     AGINHLNQLMQELLNPLQGQTEFLFNDTHFRKGDKVLHLVNDAQLNVFNGDIGYITDL
                     IPAKYTESKQDELILDFDGSEVTYPRNEWLKLTLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRLLQRNVIYTAITRSKSKLILLGEYTAFEYAIKHEGDKRQTYLIERFQEQSDLASS
                     QPNQELKSKEQTSLFSNTATLEDDSQKSSSQSTNSNPTENSQSDNDDFRLTPENYSTI
                     DSMIGLTESDIALFFQKKS"
     gene            complement(16302..16895)
                     /gene="lepB"
                     /locus_tag="ACGEUI_001300"
     CDS             complement(16302..16895)
                     /gene="lepB"
                     /locus_tag="ACGEUI_001300"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8166136.1"
                     /translation="MKQFIKEWGPFTLFLILFGLSRLFLWQAVKVDGHSMDPTLAHGE
                     RLIVFNQARIDRFDIVVAQEEENGQKKEIVKRVIGLPGDTISYNDDTLYINGKKTVEP
                     YLAEYLKQFKNDKLQKTYAYNTLFQQLAETSDAFTTNSEGQTRFEMSVPKGEYLLLGD
                     DRIVSRDSREVGSFKKENLIGEVKARFWPLNKMTVFN"
     gene            complement(16906..17793)
                     /gene="rnhC"
                     /locus_tag="ACGEUI_001301"
     CDS             complement(16906..17793)
                     /gene="rnhC"
                     /locus_tag="ACGEUI_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFF8166137.1"
                     /translation="MKIDAILSKHLKKQLAPYTISSQNTYVAFAAKKNGVTVLLYKSG
                     KLVLQGNGANALAQELNLPVAKTVFEASNNSQDIPIIGSDEVGNGSYFGGIAVVASFV
                     DPKDHSFLKKLGVDDSKKLSDKTIQQIAPLLEKQIPHQSLLLSPKKYNELVGKSKPYN
                     AISIKVALHNQAIFLLLQKGIQPKQIVIDAFTSQSNYEKHLKKEKNHFPNPLTFQEKA
                     ESHYLAVAVSSIIARNLFLDNLDQLGQDLGYQLPSGAGSASDKVASQLLAAYGMSSLE
                     YSAKLHFANTHKAQALLTK"
BASE COUNT         5348 a         3175 c         3638 g         5633 t
ORIGIN      
        1 taaagcaaaa ggtatcgtaa aagatggtct aaaaactctc taaaaacttg acttcataaa
       61 aaccgaagtt tataataaac ttaacatgat taactaatct cattttagtg tggagggaaa
      121 attgtgacca aatcaacttt aaaagagtta agaacaacaa agaaaatgac acagcaggaa
      181 ttgtctcact taacaggtat ttctgtcaga acgattgcta ggtatgaaaa agatactaaa
      241 aagctaaggc gagcaaaata tgagaagcta aaaggtattg cacaagcatt agcggtttcc
      301 gttgatgata tttttttagg aatagattcg gaatttatga actaagacga tcgttttctg
      361 tttgctatta gtaagaccaa ttaacaaaaa agtgcttagt tatattgcta agcacttttc
      421 tttgttaatt tgatgctatt ggcacaatca attgagactg actgagatcg atagttaaag
      481 catagttgct gttgtcacgg acagtatgtt caaaatcggt tgtgtataag atgactcgaa
      541 gggtatctcc tttttctaga tgatagatgg ttggttgcaa aggaagtcta acagtcatcc
      601 atttattatt aggaatagtt tcaattgacg acaaattatt gcgattttgt aaattcatga
      661 aacctttcga aataacacgg taagaatctt ctctaaatgg tagctcttta aggggttccc
      721 tagagaaatt ttgcccattg tcaatacttg attgggtgag agcaatagga agatcgccca
      781 agcgtttttt cttaccatag tcaaggattt gtgcagatag aagtccttta ttttcacttg
      841 atttcacttt taactgtagg acaatttcac cgttaatagg taagtcctct tccagtaaaa
      901 tatcaatcag ggcttgattg gccttgcctt taaagagagc tgctttgaac gcacggaaat
      961 ctttaccgta agctttaaat tcatcttcac catagtgatt atcaattaaa agcagttcct
     1021 gaccgaggtc aagctctttg atggttgagg ttccaaagac tttacgctcc tgccagtttt
     1081 gtgggcaggt gttatcctgc cagatcattt caggaaggct aaaatcatta gctaggccta
     1141 agagtttttg acataaaagg gcattcatgc tttcgcgaaa atcaatggat tgccaattat
     1201 gcatgtaaac atgctcacct tgatgtaaaa agaggtgctt gttgattgtg gaaggaagcg
     1261 cattgaaaat ctcatagacc tgtcttggtt tgacattcca gtcttgtaag ccatgagtat
     1321 aaacgacatc acactttatt tgatgagcat tttttaagta attgcggtcg tgccaaaatt
     1381 gattataatc accagattgt cggtctaacg cctgagactg ttggtttagc aattcttgat
     1441 aacgatcatt atggcgaaga taatcgccag ccaataaatt acgcgagtag gtgagctcag
     1501 ttaggacatc tagatcctct ccaggatagc caccaggact acaaactaag ccattttcgc
     1561 ggtagtagtt gtaccaagaa gaaatagctg attctgcaat aatcatggct aatccgtcta
     1621 cgcctgtagt ggctaaacca gtagacatag ttcccaagta ggactttcca gtcgtacaaa
     1681 ctagaccatt tgcccaatca gctctcactt ggtgggtttt tgagtggctg gtgtaggctg
     1741 tggctcttcc atttagccaa tcaataacgg ctttaaagct ttcaatttga gcatagtttc
     1801 cactcgtcat aaagccagtg gaaccagcag ttcccacacc tgaaacatag atattagcaa
     1861 acccacgtgc gaggaaataa tcgttgaggc tataggagtt aatgtagcta aagctttcta
     1921 gattttgacc tataggaagt ttgcaaggct gtgtctcaag aggaataaaa tccctgtctt
     1981 cgacagtgat ttgtctcctt tttttaacga ctaactcttt ttccatacga taaagttttt
     2041 tgtcgttggc tacttcattg ataccttgat ggtatggact aggtgtcatc aaagttggta
     2101 atggtttttg agatggagga cgaatgatat taaccttaat aagatcaagt tggccatctt
     2161 gatcggtatc aagaggtgct tcaacgtaaa caacttctcg aatcagctgt gacgtgtcaa
     2221 aggtagctaa tgatttaccg ttgaagaagt ggtaatcatt gtccatagta agcattcctt
     2281 ggctgaccaa atcatcaatc agggtcatgc cagacttaca acgggtcgct aataagtggt
     2341 ggatatgctt gataatttct gtttcgtcaa agtggatagg aaaggccagt ttatcaagga
     2401 aagcatcggc ctctgtaaaa tcaactccag gaataaagcc aaggacttgc agggccagta
     2461 aatcaaaaac ctctttggat aactcttgct ctgattgaaa aaagctcaat gcgtccatgt
     2521 catttttggc gattagatga cttaaaggat aatcactatc agggtagtgg aagaagagtt
     2581 tacgtaaaaa gctttctagg ctagctttgg cagttttttg cagatccaga tcaaagccca
     2641 attcttttaa ttcttcagcc gctctttcta gactagttgg aatataggaa aattgattat
     2701 agcgcatagg agtttccttt ctagtggctt agaaaacgat attatctttt aatttatcaa
     2761 gattggtaat gataatatgt tgtttatcaa tgcgaatgat gtttttttct ttcaactgtt
     2821 ttagaatacg gctgacgctg ctagcggtag aaatgccaca gaatttaccg agttcctcat
     2881 tagtaatgac aaaattaatt aaaatttggc catttggtag ttcttcgcca aatagggtca
     2941 ttaagtcata tatttgagta gaaatggcac cgatacggcc gttagttaac atacattgca
     3001 tttttttcat ggatttttga agacgattat ggtaaaagtc cttcacatat ccttgtagtt
     3061 caatatcttc gtttatatct tttaaaaatg ctgagcggcg aaccttgtaa aaactagctt
     3121 tctctgactc gatgcgaaca ttataaggct ctcccatatc ttttgaataa ccagtgttga
     3181 gaacagaagt aatttcaagt cctgtaacat atctcaaatt aaattccatt ccatattttg
     3241 aaagaacact ctgtttcaca atgccgtctt ttaaaatata agtgaagtcc tcttcgagac
     3301 tttcaaaagt caagtacttg tgataacttt tttcaatgat aggaaagtta tgattatcaa
     3361 tataccgttg caaaatgtga tgattcacgc ttttctccaa atattctttt accaatgtat
     3421 aatttgtatc ttcaagagaa attaacattt gctaatgccc tttgatagta acacttatat
     3481 tataccattg aaatatatta tattctgctt tttttgtaaa atgtttatga aaatagagag
     3541 gagttcattt tcaatggaag aaaaattgtt taacaaacat tttgtggcca tcacagtaat
     3601 aaactttata gtctatatgg tctactatct ttttacagtt attattgcct ttgtggcgac
     3661 tagagagcta ggagctcaga ctagccaggc aggattggca acagggattt atattctagg
     3721 gactttatta gcccgtttga tttttggaaa gcagttggag gtgtttggtc gtcgtttggt
     3781 tttaagagga ggtgctattt tttacctctt aacgacctta gcctactttt atatgccaac
     3841 gattagcatg atgtacttgg ttcgtttctt gaacggattt ggttatggtg tagtatcaac
     3901 tgcgacaaat accattgtaa cagcctatat tccagctcgt aaaagaggag aaggaattaa
     3961 tttttatggc ttgtcaacca gtctagcagc agctattggc ccttttgtag gaacctttat
     4021 gttggataat cttcatattg attttcgcat gattattgtc ttatgtagcg tcttgattgg
     4081 gtgtgtggtt gttggtgctt ttgcttttcc agtcaaaaac atgtctttaa atgcggagca
     4141 attggctaaa accaaatcgt ggactgtgga tagtttcatt gagaaaaaag ccttgtttat
     4201 cactgcaatc gcctttttaa tggggattgc ctatgcttca gttttaggat ttcaaaaatt
     4261 atacacgtct gaaattcatt tgacaacagt aggcgcttat ttctttgtgg tctatgcctt
     4321 aatcattacc atcactcgtc cagcaatggg acgtttaatg gatgctaaag gcgataaatg
     4381 ggtcttgtac ccaagttatc tttttttagc aatgggactc ttcttgctag gtagtgtatc
     4441 gtctggagga agctatttgc tttcaggagc cttgattggc tttggttatg gcacttttat
     4501 gtcttgtggt caagccgcct ccatacaagg agtggatgag caccgcttta atacggctat
     4561 gtctacttat atgattggtc ttgatttagg tttaggtgct gggccttacc tcttgggctt
     4621 gattaaagat ttggcacttg gtagtggtgt ggcttccttt aggcacttat tctggttagc
     4681 tgctgtgatt ccactgattt gtaccttgct ttacttgtta aaaacaaaga caagacaggt
     4741 tgtgtcttaa ggacaagaaa aggagtttag tcttatactt aaatacaaac gtatcttaac
     4801 agctgtagat agttcctatg agccgggatt agctgtgggt aaagccatta ctatagcgct
     4861 ttgaaataat cctagtttgc tattggtaca tgtcaataaa aatgactcgc aaaagccatt
     4921 cgtgaaagag aaaatgtggc cttattttta gtaggtacaa ttggattaag cacgtttaga
     4981 tacatgacaa ttggattaac atcagaatat aggctttgtc atgttaagat tgatctgttg
     5041 atagtgagag ctaataaaaa atgttataat actatgaaat aaagtgataa agataaggcc
     5101 ttttatggtc cttttcttta ccgcttttat tattgtgtga aaataacact taatgatcat
     5161 tttatttatt attttattac ctttacattc atcaataagc ctgttaaaat ggcatttgtt
     5221 atattttatt atttggagga aaccagaaaa catggaaatg acatggactg tgaaatacat
     5281 cacagaattt atcgcaacgg cttttttgat tattttggga aatggagctg ttgctaacgt
     5341 cgacttaaaa ggaacaaaag gacataactc aggttggtta gtgattgctt ttggctatgg
     5401 cttaggagtt atgatgccag ctttgatgtt tggtaatgta tctggaaacc atattaatcc
     5461 tgctttcaca gttggactag cagtatcagg cttgttccca tgggctcacg tcttacaata
     5521 cgttgtggca caattacttg gagctatctt tggtcagttg gtagtggtaa tggtttataa
     5581 accttacttt atgaaaactg aaaatccaaa tcatgtttta ggctcattct caacgatttc
     5641 atctcttgat aatggtcaaa aagacagtca taaagcctct tatatcaatg gtttcttaaa
     5701 cgaatttgta ggttcatttg ttcttttctt tggtgctttg gctttgacta aaaactactt
     5761 tggggtagag ttagttggta aattgattga agcaggctac gaccaaacaa ctgcagcaac
     5821 acaaatttca ccatacgtga caggctcatt ggcggttgct cacattggta ttggtttctt
     5881 agttatggtc ttggttactt cattaggtgg accaactggg ccagcactaa acccagcgcg
     5941 tgattttggg cctcgtttgt tacaccactt cttaccaaaa tcagttcttg gtcaagccaa
     6001 aggtgattca aaatggtggt atgcatgggt tcctgtagtt gcacctatct tagcggcgat
     6061 agtagccgta gcagcgttta aataccttta tatcagataa aaaaagaact aaagcttctt
     6121 ttagactcaa gacaagctcg cttaaggaac ttgtcttttt tatatgagtt aagcgaaatc
     6181 taatacaaac ttgactgtct tgtatagcgt attttgtaaa ttttattaga cttcttgtaa
     6241 aaagcctctc ctcagataag atgactaatg cctctaaacg gtgttacaaa taaacgaggc
     6301 atctctaact tcctttatct ggtgttgaaa ctattttgca cggactaaat cactgataga
     6361 gtcaaaaaac aatttttcat ttttgttgta aaatattaaa aacgtctctg gttagtgatt
     6421 cccagaaacg ttatcttatc tatttgtttg ttgcttatga gaggtaaagg aggaacgaat
     6481 cagcattcca ccagtgaaga aaacgatagc gatgaccaga agtttcaagc tattaatatc
     6541 taaattcgtt aagaaaaaca cagctagtaa aacgccaata ataccaccag aaacaattcc
     6601 agcaaaacca tcccaactga cacggtttgc tttgataaac tggctactag aagctgtcat
     6661 aatcatagct gcatctagca tcataacagg catagcaact gttgggctaa gacccattaa
     6721 tgaaaagaaa atcaattctg gagcataatt gcctaacccc atagtcatca agactccaac
     6781 gataaaatta aaaccaattc caacaaaaag ccagatgcca tggaggccat gaatggtgtc
     6841 tgaaaggtgt tctcctggat ggtagattat tctaatgatc atgattatag ctgctatgat
     6901 taaaagacta ccaaggattc gttggacagt tggagcatgc cagttttttg taatatgagt
     6961 cccaaaataa gctccaataa aggctgctgc agccatagca agtaaagtta gaacctcaac
     7021 ttttacgaca aaaataaagc aaagagattg aatgagcacc ggaagcacat gagctacggt
     7081 catagtgcca ggcaattgtc tatcgtcagt gactaattta gttaacttaa agcacgttgt
     7141 ggtagtagca aaagacccta tcccaagggt gtcaagggca tctgttagca ggccaatcca
     7201 aaaccccgtc caaaagcgtt taaaaggatt gatcttattt tttttaagat gttttactaa
     7261 cataaagaaa ataaaaagca ttgccgaaac tagcaaagct tgtatcagtc taaggataag
     7321 catttcgttc ataaagacta gtttacaaaa aaaaacctta gccgtcaacg tgatttactt
     7381 cattaaaaca tgccactatg cttagaagag ctttagttcg atgtattttg ttaaaattaa
     7441 gctaaaatat taatattgca tgaaagggga cgccttcctt tttcccttac tgagcttagc
     7501 tgttattgta gtgaatgttt aaaaggctag tttgtcgctc tttatcaaaa atctagtagg
     7561 atgaatacgt ataatttctt gtcaaataga ggaattttgt ctataatagc agaggattag
     7621 gaggaaatta tgactttaat ggaaaatatt acctttaaaa ctccagtatt acgcgttaat
     7681 gatcgcgacc ttaatattgc attttatcaa aataatttag ggcttcgttt agtatctgaa
     7741 gaaaatgcga ttgctatctt ttcatcttgg ggagaagggc aggaatgttt tgtaattgaa
     7801 gagtcacctt cagttagaac gcgtgcggta gaaggcccta aaaaggttaa tacgattgtc
     7861 atcaaaacaa atcaacctaa agaaatcgaa caattattag ctcatggagc acattatgac
     7921 gctcttttta aaggtcaaaa tggctatgcc tttgagacta tttccccaga aggagaccga
     7981 ttcttgttgc atgctgaaca agatattaaa catctccaag gtactgattt accatcgctt
     8041 gaaaaagatg cgacttttaa aggtttaact caatttaagt ttgatattat tgttttaaat
     8101 gttattagtg aagaacgctc aaaagctttt tatcgggatc tgtttagtga tcagttacct
     8161 attactatgg attttattca agaagaaggt cccgatttgg caattgatcc ccatattgct
     8221 tgggatttag aaattttaga atttcaagtc tcaaaggact atgatatgaa ggtcctaaaa
     8281 gcaactttag aagaagacgg gcacaaggtc tacattgata aaaaacacaa agtattggtc
     8341 ctttctgatc caagtcagat cgaagtatgg tttacgaaat gactttggcg gtcattaaat
     8401 gtattgaaaa ccatcttcac aaaaaagtat acaaaggggc cagtctggct ctctttcaat
     8461 ctggtcgatg gcaagaatac catatcggaa caattgatgg aagacgacca gttgatgcta
     8521 acttagttta tgacttggct agtgtttcaa aagttgttgg tgtggccaca atatgcaata
     8581 ttttattgaa taatggtacg ttagcattag atgatccctt aaaggtatat tatcctagta
     8641 ttgctgatgc gactgtcact attcgacagt tattgactca taccagtggt cttgatcctt
     8701 atattcctaa cagagatgtt ttaaatgcac aacaattaag aaaagcactt aatcatctta
     8761 ctcaaaaaga aaataaaaac ttttattaca cagatgtcaa tttcctcttg ttaggcttca
     8821 tgttagaaga gctctttagt gaatctttag accagatatt tgacaagact atctttactc
     8881 cctttgggat gtatcatacg tcttttggcc ctcgtccaga agctgtacca acattaaagg
     8941 gtgtgagtga tggagaagtt catgatccca aagcaaaaat cttaaaaaaa cattctggat
     9001 ctgcggggct attttcaact cttgcagatt tagaaagttt tagcaatcat tatcttaatg
     9061 atccattttc agattgtctg tggcgcaact acagccaaca aacaattgag cggtcactag
     9121 ggtggaattt agatggcgat tggattagtc atacaggtta cacagggccg tttctaatgc
     9181 ttaataaaaa agagcaaacg gcagctattt ttttgaccaa ccgtacttac gacgaggatg
     9241 ataaaagtaa atggttaaaa gaacgtcagt tgctttacaa tgcactaaag catgatctga
     9301 caacccccgt atcgtaacgt ctaaaggggc agttttagtg atcttattac tgtttttaaa
     9361 gtttcttcta aaatagccat ttcatttgtc tgatgcgatt agagtgatta taagtgaaaa
     9421 aagaatagcc atctcatttt gaatcaaatg agatggctgt tcttttttat aagttattga
     9481 tcatttagat actactattt acttgaaata gcttcagcag catcgtccat ggaaagcact
     9541 tcgtggaaga cacgttgtgt caattcagtt ttttgttctg gtgtaaggta tttggtgttg
     9601 acacagtaac cagagatacg tacgataacg tcttcgccgt tcatgatttt gtcataaaca
     9661 tcgttaaggt ccataacgtt caagttaacg tgctgaccac cattttcaaa gtaaccgtca
     9721 agcactgtta ccaagttgtc aacttgttcg tcaaatgttt taccaagagc acgaggtgat
     9781 acttgagtag tcaatgagat accatcgtta gcatgtgaga attccagttt agcaagtgag
     9841 ttaaggtttt gtaaccagcc accttttgct ttgttagatg ggttagcacc tggtgagaag
     9901 aattccactt ggctagtgtt aactgtacca tcttcgttta agaagacgcc gcggtgaact
     9961 ggagagttac cagtttgttt agagtaagca acgtttgaag tgatggtaag aagtgatact
    10021 gaagcctcag cattcttgta aagtttgtgg cttgcaagac gtgtgtggta agcttccatc
    10081 aaccatttcg cgatgtcatc aacacggtcg tcatcttcac cgtaacgagg gaagtcacct
    10141 gttacttcgt agtcatagat gtagccattt tcatcgcgga tagttttaac agttgcgtat
    10201 ttaatagctg acaaggtatc aactgtgtta gcgaaaccac agataccgaa tcccatgtta
    10261 gcacgttggt gtgttggcaa gaaggccatt tgaactgctt cgtagttgta tttatcagtc
    10321 atgtagtgga tgatgttaag cgcatctaca taagtatctg tcaaccagtc aagagatttc
    10381 tcaaagttag ccataacttc gtcgtactca agaacttctg atgtgattgg ttcaacaacg
    10441 ttaaacactt tgtagtctct gtgaacatca tcgtaaccac cgttaagacc agtaagaagg
    10501 gctttaagaa cgtttacacg agcaccgaag tattggatgt tatgacgttg ttcttcattt
    10561 tctgggtcaa gtggtgacac acaacatgaa atacatgaca tttcaccata accttcttta
    10621 gccattgttg tcacaccttc atattggata gaagagtgct tgtggctcat tttcatacag
    10681 tagcgacgga atgtttctgg cagttggtca gtccaaagaa cagtcaagtt tggttctgga
    10741 gagttaccaa tattatcaag cgtgttcaag aaacggtagt ccatcttagt tacacggtga
    10801 cgaccatcgt tacccatacc agccatagaa gttgtgatga atgttgggtc acctgagtaa
    10861 agcgcatcgt aagctttcgt acgaccaaat tttacggtac ggagtttaag gacaaaatca
    10921 tcaacaaatt cttggatttc tgattctgta aaggtaccgc gagcaaggtc acgttctgca
    10981 aagatatcaa gaacgattgg cacacgtcca agagaagtag cagcaccgtt gatcacacga
    11041 catacagcca tgaaagcgat gtttacccat tggatagctt ctttaacgtt ttgtgctggg
    11101 cgacgaacat caactccgta aaggtcacca agtttaacca cttcaccaag agcttggtat
    11161 tgaaggttaa cttcttcgcg aagacgaatg gattcttcat cgatttcagt gatagcattc
    11221 cagtcattga ctttttcttg catcaagtag tcagcaccgt aaagagcaag acgagcgtaa
    11281 acaccgatga tacgtccacg tgagtaagca tctggaagac cagttacagt gtgggcatga
    11341 cgagcgcgac gaatatttga agtataagcg cggaagatac cgtcgttaac agttgtcaca
    11401 tacttagtaa agatttcatg tactgctggg tctggttcat agccgttttc tttaagggtc
    11461 gtttccgcca tacggatacc accttttggc atgaagttca atttgaacaa ttcatcgttt
    11521 tgaataccat aaattaattc attttcttta tcgatgaaac cagcaggaat gtcagcaatt
    11581 gaagttggac gagtatcgta tgggaaacgt gtagcttcat agtgtgcttt tgtttcttcg
    11641 ataacttttt tgatgtgaag tgaacgttca gtagcaccag ccaagaaaga ttcatcgcca
    11701 tcgtatgggg tgtagttagc ttgtacaaaa cgtgagacac ttgctttttc tttccagtca
    11761 gttcctttaa agccttccca agctttttcg aaaacatctg tattggtttt aacagttgcc
    11821 ataaatttct ccttaaattc tagtaacaaa gcgatctgtt acatttacaa ttctaagttt
    11881 aacacatagg aaacgttttc ccaagcaaaa ttcttgacaa aattaagatt cttttataat
    11941 acagattgtt gaaacagtca caaactgtat tagaatgtat ttttttggct ttagatagcc
    12001 taaaaggtat aatagagaaa ggagggactt atgcttattt ttccactgat taatgacacg
    12061 tcacgaaaaa tcatccatat tgacatggat gccttttttg ctgcagttga ggaaagggat
    12121 aaccctgctt taaaaggaaa gcctgttgtg attgggaaag atccaagaga aacaggtggt
    12181 cgcggagttg tttccacttg taattacgaa gcgagaaaat atggcattca ttcggccatg
    12241 agctctaagg aagcttatga gcgttgtccc aaagccattt ttatttcagg aaattatgaa
    12301 aagtatcgaa cagttggaga ccagatccgc cgtattttta agcgttatac tgatgtggta
    12361 gagcctatgt ccattgacga ggcttacctt gatgtgactg ataataagtt ggggattaag
    12421 tcagccgtca aaatagccaa gctgattcag catgatatct ggaaagaagt aggattgacc
    12481 tgttcagcag gtgtgtctta taacaaattt ttggctaaat tggctagtga ttttgaaaaa
    12541 cctcatggcc tcactctagt cttgaaagaa gatgccctgt gctttttagc caaactcccc
    12601 attgaaaagt ttcatggtgt tggtaaaaaa tcagttaaaa aactgcatga catggggatt
    12661 tatacaggac aggatttgtt ggcagttcct gaaatgacct tgattgatca ttttggtcgg
    12721 tttggttttg acctttaccg taaagcgaga ggcatcagca attctcctgt caagtctgat
    12781 cggatacgca agtcaattgg cagtgagaga acctacgcta aactgcttta tcaagaaaca
    12841 gacatcaagg cagagatcag taaaaatgtt aagcgcgtgg ccgctctctt acaagaccat
    12901 aaaaagttag gcaagaccat tgtgctcaaa gtgcgttatg ctgattttac caccttgaca
    12961 aaacgtgtca ccttgccaga attaaccaga aatgccgcac aaattgagca agtagctggg
    13021 gatatttttg acagcttaag cgaaaatcct gctggtatcc gcctgttagg ggtaaccatg
    13081 accaatttgg aggataaggt agctgatatt tccttggacc tataggagaa agagtgctct
    13141 atccgtccgc ctcattttgg agaaaaaaga agctgattag ggatttccct aatcagcttc
    13201 tttggtccat tattttatta cagcatagct tggtctgtat catcaccttt ttcgttaagg
    13261 gttttagcct tgtaaaaagc aaagccataa agagcagctt gtataaggat aaggatagtt
    13321 ccaaccattg acatggttgt tgtgaactgg ttaaccaagc cgataacaga aaggataagg
    13381 cctaagatat aaggaatata actaaccgtt agaccttgtt ttaatttaga taagttttga
    13441 gcacaaaaga caatgatagc aataatagca aggacattta agacagataa gaaaatcata
    13501 aatggtgtca tgctgctttg tagctgtgcc aattgctcag ttgttaattg ggcccgaaat
    13561 gcttcattct tgagttggct ttgtaagtag aaaatccctg caaatcccat taatgacagt
    13621 acaactccaa gcccgtttag aataataatg gcaatagtag acgttttgag tgcctgacgc
    13681 actttttcat aagaaatcat ataatcctcc tagagtcctt aatccgaaat tgtacaatgt
    13741 gtttaataca aaaatattat atcatagatt aatcaaaaat gtccgttttt ctaggatttt
    13801 ttctggaaaa agagggcaat atctgattct gtaagcccta tcatcgaatc gatagtcgaa
    13861 taattctcag gtgttaacct aaaatcatca ttatctgact gagagttctc agtggggtta
    13921 gagtttgttg attgagagga agatttttga gagtcgtcct caagggttgc cgtattagaa
    13981 aaaagggagg tctgctcttt tgattttagc tcttggttag gttgagagga ggctaagtcg
    14041 gattgttctt ggaagcgttc gatcaagtag gtttggcgtt tatcgccttc gtgtttaata
    14101 gcatactcaa aggcagtata ttctcccaac agaattaact tacttttaga ccgagtaatg
    14161 gccgtgtaaa tcacatttcg ttgcaagagt cggccgcttt ggcgtgtgat aggtaaaatc
    14221 accacttgaa actcactccc ttgcgattta tgaatgctca tggcataggc cagggttaat
    14281 tttagccatt catttctagg gtacgtgact tcgctaccgt caaaatctaa gattaattcg
    14341 tcttgcttag attcggtgta tttagcagga atcaaatctg taatataacc aatatctccg
    14401 ttaaagacat tcaactgagc atcgttgact aagtgcagga ctttatcacc tttacgaaaa
    14461 tgcgtatcat tgaacagaaa ttctgtttgt ccttgcaagg gattgagaag ttcttgcatg
    14521 agttgattaa gatgattgat gccagcttgt cctttgtaca taggtgctaa aatttgaatt
    14581 tcctcagcag ggatgccact tttaatagca gataggacaa ttttttgaat catgtcaggg
    14641 ataaaggctg cttgggcttc aaaataagaa cggtcagctt ttttatcacg aaagtcagca
    14701 gctaagattc ctcgacgcat ctggtctgcc aaattgacaa tagtagattc ttgggattga
    14761 cgaaatatct tttgcaaggc gatttggggc aggctattga cttttaaaag atctgacagg
    14821 acttgaccgg gtccaaccga aggaagctgg tcactatctc cgacaataat cacttgagtt
    14881 gttgagttaa tagctcctaa caactggttg gcaagccagg tatccaccat tgaaaattca
    14941 tcgacaatca gcaggtcgca gtctagataa tcctccatgg cttggtaatc gttgtcgcca
    15001 ttgaggccta agtgcctgtg gatggttgcg ctaggaagtc cagtcaactc attcatgcga
    15061 cgtgcagccc gacctgttgg agctgctaac aagataggaa ggtctttttt atccaaatca
    15121 atctgatgca ggttcgcgta agcctgcaaa atgcctcgaa taacagttgt ttttcctgtt
    15181 ccgggaccac ctgttaggag aaagaccttg ctggttaaag ccttagtaat ggcttcttgc
    15241 tgcacttggt cataggtgat ggcaaagtct ttttgaacgg cttggatggt tgtttgaatg
    15301 gtatcatgac taaatgactg acttaaagga gtatctaaga gacgagagat gttgttagca
    15361 atcccttctt cagcaaaata aaggctagca tcaaacagtt tggtgtcact gtttttgatt
    15421 ttgccctcta tgattaactc gcttagttgt tcagccacag cagcaggatc acattctact
    15481 tgacgtgcat cttcaaggag tgtgatggca aagtctaata attgtcgtgc ttggacataa
    15541 gtatctcctc gattgatgga ttcttccaag agacagtgaa ggagggctgc acgaaaacgt
    15601 ttcggagagt cgctttcaat ccctaagttt tcagcgagag catctgccat tttaaaccca
    15661 aaaccttgta gatcttcgac taactgatag ggattttctt taacaaggtc aagagcctct
    15721 tctttatact tttcaaacgc ttgaagggca aaacggttgc taaggccaag ttcgacgaga
    15781 ccagcaatca gttgctctgt gccgtaattc aattttagtt tagcgacaaa ggcttgacga
    15841 ttagccttag ataggccaga gatagtttct aatttgcttg ggtcttctaa aatatggtca
    15901 atggtattat gaccatatag cgcaatgatt ttctccgctg tttttttacc aatcccctta
    15961 aaatggtcgc tagagaaata attaaccaaa ccagatgaac taggtttgat tttttggtaa
    16021 cggcttagtt tgagttgctg tccatattta gggtgctggg tcaattcccc ccaaaaggtg
    16081 tagtcatctc cttcaataat gtcagccatc gttcctgtga taatgatttc aaagtcgtca
    16141 atgtcactgt ctgtatcctc aatggcaagg aggagaattt taaagaaatt agcctggttt
    16201 tcaaaaatga tacgatcaac agtacctgta aaaacgtatt ccatagtaac tctttctatt
    16261 tatacaagat aactgaaaac ctgcccaaag gcaggttttt tctaattaaa aacggtcatt
    16321 ttattgagtg gccaaaaacg agctttcact tcaccgataa ggttttcttt tttgaaacta
    16381 ccaacttcgc gactatccct ggaaacaata cgatcatcac caagaagaag gtattctcct
    16441 tttggaacac tcatctcaaa gcgtgtttgt ccctcagaat tagttgtaaa agcatcagat
    16501 gtttctgcta actgttggaa tagggtatta taggcgtaag ttttttggag tttatcgttt
    16561 ttaaattgtt ttagatactc agccaaatac ggctcaactg tttttttacc attaatataa
    16621 agtgtgtcat cattataaga aatggtatcg cctggcaatc caataactct ttttacgatt
    16681 tctttctttt gtccgttttc ttcttcctga gcaactacaa tatcaaagcg atcaattcta
    16741 gcttgattaa aaacgataag gcgttcgcca tgagctagag ttgggtccat agaatggccg
    16801 tctactttaa cagcctgcca caaaaaaaga cgagatagac caaagagaat taaaaagaga
    16861 gtgaatgggc cccattcttt aataaactgt ttcatatgat tccttttatt tagtgagaag
    16921 cgcttgcgct ttgtgagtgt tggcgaagtg aagtttagca ctatattcta agctagacat
    16981 accataagcg gctaagagtt ggctagcaac tttatctgaa gctgaaccag caccgcttgg
    17041 gagttggtaa cctaaatctt gacctaattg atcgagattg tctaaaaata gatttctagc
    17101 aatgatagag ctaacagcca cagccaaata gtgactttct gccttttctt gaaaggttag
    17161 aggatttggg aaatggtttt tttccttttt tagatgcttt tcataatttg attggctagt
    17221 gaaagcatca ataacaattt gtttaggttg aatgcctttt tgtaacaaca aaaaaatagc
    17281 ttggttatga agagcaactt tgatagaaat ggcattatat ggtttacttt taccaaccaa
    17341 ttcgttatat tttttaggtg ataggagaag tgattgatga ggtatttgtt tctctaacag
    17401 aggcgcaatc tgttgaatgg ttttatccga taatttttta gaatcatcaa ccccaagctt
    17461 tttcaggaag gaatgatctt taggatcgac aaaacttgcc acaacagcaa tcccaccaaa
    17521 ataagagcca ttacccacct catcactgcc gattataggt atatcttggc tattgttgct
    17581 tgcttcaaaa actgtcttag ctactggaag attaagctct tgagctaaag catttgcacc
    17641 gtttccctgg agaactaatt ttccggattt gtacagcaaa acagtaaccc catttttttt
    17701 agcagcaaag gctacatagg tattttggct actgatggtg tagggggcta gctgtttttt
    17761 taaatgttta ctcagtatag catctatttt caaa
//
LOCUS       DAXWIQ010000036        16414 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000036 DAXWIQ010000000
VERSION     DAXWIQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16414
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.036"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            18..98
                     /locus_tag="ACGEUI_001302"
     tRNA            18..98
                     /locus_tag="ACGEUI_001302"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52..54,aa:Tyr,seq:gta)
     gene            105..175
                     /locus_tag="ACGEUI_001303"
     tRNA            105..175
                     /locus_tag="ACGEUI_001303"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:137..139,aa:Trp,seq:cca)
     gene            187..259
                     /locus_tag="ACGEUI_001304"
     tRNA            187..259
                     /locus_tag="ACGEUI_001304"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:220..222,aa:His,seq:gtg)
     gene            268..339
                     /locus_tag="ACGEUI_001305"
     tRNA            268..339
                     /locus_tag="ACGEUI_001305"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Gln,seq:ttg)
     gene            359..442
                     /locus_tag="ACGEUI_001306"
     tRNA            359..442
                     /locus_tag="ACGEUI_001306"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:393..395,aa:Leu,seq:caa)
     gene            1300..2139
                     /gene="ispE"
                     /locus_tag="ACGEUI_001307"
                     /pseudo
     CDS             1300..2139
                     /gene="ispE"
                     /locus_tag="ACGEUI_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514767.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
     gene            2208..2651
                     /locus_tag="ACGEUI_001308"
     CDS             2208..2651
                     /locus_tag="ACGEUI_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HFF8166138.1"
                     /translation="MGILEKKLDNLVNTILLKAENQHELLFGACQSDVKLTNTQEHIL
                     MLLSQQRLTNTDLAKALNISQAAVTKAIKSLVKQDMLAGTKDTVDARVTYFELTELAK
                     PIASEHTHHHDETLNVYNRLLQKFSAKELEIVDKFVTVFAEELEG"
     gene            2655..3374
                     /locus_tag="ACGEUI_001309"
     CDS             2655..3374
                     /locus_tag="ACGEUI_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8166139.1"
                     /translation="MRYISVKNLSFQYESEPVLEGITYHLDSGEFVTMTGENGAAKST
                     LIKATLGILQPKAGRVTIAKKNKDGKQLRIAYLPQQVASFNAGFPSTVYEFVKSGRYP
                     RSGWFRHLNKHDEEHVQASLEAVGMWENRHKRIGSLSGGQKQRVVIARMFASDPDIFV
                     LDEPTTGMDSGTTDTFYELMHHSAHQHGKSVLMITHDPEEVKAYADRNIHLVRNQKLP
                     WRCFNIHEAETDDEKGGHGHA"
     gene            3367..4182
                     /locus_tag="ACGEUI_001310"
     CDS             3367..4182
                     /locus_tag="ACGEUI_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFF8166140.1"
                     /translation="MLDILFYDFMQRAVMAVVAISIFAPILGIFLILRRQSLMSDTLS
                     HVSLAGVALGVVLGISPTITTIIVVVLAAILLEYLRVVYKHYMEISTAILMSLGLALS
                     LIIMSKSHSSSSMSLEQYLFGSIITISMEQVVALFAIAAIILILTVLFIRPMYILTFD
                     EDTAFVDGLPVRLMSVLFNIVTGVAIALTIPAAGALLVSTIMVLPASIAMRLGKNFKT
                     VILLGIVIGFSGMLSGIFLSYFFETPASATITMIFISIFLLVSLGGMLKKRLF"
     gene            complement(4222..4605)
                     /locus_tag="ACGEUI_001311"
     CDS             complement(4222..4605)
                     /locus_tag="ACGEUI_001311"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFF8166141.1"
                     /translation="MTECIFCHQLKANQLLTQSQYFKVVFDIDPIQKGHLLLISKDHY
                     TSLTQLPKEGHYDLIDLQAALVAKLEQHLPISGVTSVSNDKELMDEGTHFHLHLIPRL
                     TNDSFWEGITINQENWDLTPFLKHL"
     gene            complement(4656..5912)
                     /gene="tyrS"
                     /locus_tag="ACGEUI_001312"
     CDS             complement(4656..5912)
                     /gene="tyrS"
                     /locus_tag="ACGEUI_001312"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFF8166142.1"
                     /translation="MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSDKIK
                     GQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDE
                     IAEIETQFNAARHERLAQKTLAREVVTLVHGEEAYKQALNITEQLFAGNIKNLSANEL
                     KQGLSNVPNYHVQSIDNHNIVEILVAAKISPSKRQAREDVQNGAIYINGDRVQDLDYQ
                     LSNDDKIDDQLTVIRRGKKKYAVLTY"
     gene            6016..8316
                     /gene="pbp1b"
                     /locus_tag="ACGEUI_001313"
     CDS             6016..8316
                     /gene="pbp1b"
                     /locus_tag="ACGEUI_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1B"
                     /protein_id="HFF8166143.1"
                     /translation="MVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVIFLFG
                     MMGFGMAFGYLASQIESVKVPSKESLVKQVESLTMISQMNYSDNSLISTLDTDLLRTP
                     VANDAISENIKKAIVSTEDEHFQEHKGIVPKAVFRATLASVLGFGEASGGSTLTQQLV
                     KQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAGVEEA
                     ARGIFGVSAKDLTVPQAAFLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQNVLFN
                     MYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYYTVLADAKKAMYSYLIKRD
                     KVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLLDDGT
                     GTVQMGNVLTDNATGAVLGFVGGRDYALNQNNHAFNTVRSPGSSIKPIIAYGPAIDQG
                     LMGSASVLSNYPTTYSSGQKIMHADSEGTAMMPLQEALNTSWNIPAFWTQKLLREKGV
                     DVENYMTKMGYKIADYSIESLPLGGGIEVSVAQQTNAYQMLSNNGLYQKQYIVDKITA
                     SDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLANADWI
                     GKTGTTENYTDVWLVLSTPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANAINQA
                     DPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGHTFSVGGEMTTSLWSQKGPGA
                     MTYRFAIGGTDADYQKAWGNFGFRKN"
     gene            8580..12146
                     /gene="rpoB"
                     /locus_tag="ACGEUI_001314"
     CDS             8580..12146
                     /gene="rpoB"
                     /locus_tag="ACGEUI_001314"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFF8166144.1"
                     /translation="MAGHEVRYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDSG
                     LKEVFEDVLPISNFTDTMELEFVGYEFKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGESDLVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLIARFFDARRYDLAAVG
                     RYKVNKKLNIKTRLLNQIIAENLVDAETGEILVEAGTEMTRSVIESIEEHLDGDLNKF
                     VYTPNDYAVVTEPVVLQKFKVVSPIDPDRVVTIVGNANPDDKVRALTPADILAEMSYF
                     LNLAEGLGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNDVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRATGTVTNEIV
                     WLTADEEDEYTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFSASVVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAQQ
                     NGKVVFSDAEKVEIRRQDGSLDVYHITKFRRSNSGTAYNQRTLVKVGDIVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEEALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGIVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWDTVREAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDIMHVDDLEKAREKQAQETQEVSETTDEK"
     gene            12237..15878
                     /gene="rpoC"
                     /locus_tag="ACGEUI_001315"
     CDS             12237..15878
                     /gene="rpoC"
                     /locus_tag="ACGEUI_001315"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020904830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFF8166145.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEPKSLLTEREYREKLQ
                     EYGHGSFVAKMGAEAIQDLLKRVDLAAEIAELKEELKSASGQKRIKAVRRLDVLDAFN
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVAKEYAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDKDEAVMAYRNGYAHLHSRVGIAVDSMPNKPWKDNQRHKIMVTTVGKI
                     LFNDIMPEDLPYLQEPNNANLTEGTPDKYFLEPGQDIQEVIDRLDINVPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     EINKAFRRGLMTDDDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLLIRAITDGKEVTETLEERLQGRYTRKSVKHP
                     ETGEVLIGADQLITEDMARKIVDAGVEEVTIRSVFTCATRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGNVVEIEEDASTRTKKVYVQGKTGMGEYVIPFTARMKVEVGDEVNRGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPQAMNEIEVIDHTEVSA
                     EAVFTAEAE"
     gene            16030..16395
                     /locus_tag="ACGEUI_001316"
     CDS             16030..16395
                     /locus_tag="ACGEUI_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFF8166146.1"
                     /translation="MYQVIKMYGDWEPWWFIDGWQDDIIDEQQFSDWQEALDYFNQEW
                     QRMKAIFPSYHSQKNLLATFWEKEDKRWCEDCDEDLQQFHSLLLLKNKDIVPSNNYIP
                     EFEQRNDSPQVAYLCKLNL"
BASE COUNT         4755 a         3043 c         3681 g         4935 t
ORIGIN      
        1 aaagtttaat attttacgga aagatagcga agaggctaaa cgcggcggac tgtaaatccg
       61 ctccttcggg ttcgggggtt cgaatccctc tctttccatc ctaaacgggc atagtttaaa
      121 ggtagaacta aggtctccaa aaccttcagt gtgggttcga ttcctactgc ccgtgttaga
      181 aatatggcgg gtgtggtgaa gtggttaaca catcagattg tggctctgac attcgtgggt
      241 tcgattccca tcactcgcct attttattgg ggtatagcca agcggtaagg caagggactt
      301 tgactccctc atgcgttggt tcgaatccag ctaccccagt ttaaatttta tatttaaagc
      361 cggcgtggcg gaattggcag acgcgctgga ctcaaaatcc agtgtccgca aggacgtgcc
      421 ggttcgaccc cggccgccgg tatacttgaa aaacaaggtt tgtaaacctt gttttttgtt
      481 ttgatcttaa gctgtagcta cgtagtacaa tctcttatat tactgataag gtctatttaa
      541 tgcgcaagat ttgtggtatg atgaatatga ttgagtattg atgctttatg ggattaccgg
      601 taaggttttg aaaaaggttt gtcactacta ggtgcttttt taactataga aagacagtta
      661 tggattgatg tattgtaact aatgttggag gttaatggtg gttgagtttt gagatattta
      721 cactgataat cgggtggaaa ctggtcgggt atcgggtaat ggagtgtggt tcttcgttta
      781 agcaaggaga ttatcgtttg gtggttcata tttgcttgtt aaatgttagg gatgagatgc
      841 tgagtcagca aggccatgaa gataaagctg actggctaga gccttgagat gtgacgtttg
      901 gtggaagtcc tttttggtga gatggcacag catatagtaa tattagatcc tgaggaagaa
      961 ttgggacttt cattaggctt gactggggta tgacctcact ttacaattca tttcgatgat
     1021 gggtttgatg atacgttttt agttcaagac tttgtagagt tggagaattt ggttctttat
     1081 gaagaagaag tgcaggtaat tcgttgagcc agtcgtgaag atattttagg tatgattgat
     1141 gaaggttcgt ttattcctta tctgaagtat aaaataaatt tgtgctttga gatggttggc
     1201 caatataaag tccatcaatc ttagaagtaa taatttttga attttttctg aggtttgtgt
     1261 atatttgata gtgtactgat gtaagaagta aggagtacta tgatggcaat caatgagaga
     1321 gcttctgtaa aaatcaagtt aggccttgat actaagaata agcgatgaga tggttaccac
     1381 gatttatcga tggtcatgat gagtattgat ctgtgtgact atgtgacggt tgaccatata
     1441 gatgacaata agattgtctt tgcctcaaac tgtccgaaaa tacctattaa ctaagataat
     1501 gatgtttata agatagttca gctaatgaaa catcgatttc aagttaaaag aggagtttcc
     1561 gtttacttgg aaaagcggat tcccatgtgt gctggtatgg gaggaggttc tagcgatgct
     1621 gttactatca gagcattaaa tcagatggct ccttactctt agccgaaaag atatgatgga
     1681 tattggtata ccgattggta gtgatgtgcc ttattgcttg ctttcaggtt gtgcccaagt
     1741 cactggtaaa ggtgaagtgg tttgtcgcat tttggggctg ttatcgtctt gggtggtttt
     1801 ggttaaacca gattttggta tctcgacttg aacatttttt ctagatatta attgtaaaat
     1861 catttcaaga gtgtctacta ctcatttggt tgcggcgatt gaagcgggta actataatga
     1921 tgggatattg actgaaatga acaatttgct ggaagatatt ttcattgcca agcgaccctt
     1981 tattcaaaaa ataaaagaga aaacactgca agctggtgct gctaatgctt tgatgacagg
     2041 aagtggaccg actgtttttg ctttgtgcca aaccgaaaaa caaactataa tagtttaaaa
     2101 ggactttgca aggaagtttt taaggtgaga atgttgtaat attttgttgg gattgcttct
     2161 ttggaagctt catgttataa ttaactggtt aatgaaagag gagatttatg gggattttag
     2221 aaaaaaaact tgataactta gtaaatacta ttttattaaa agcagaaaat cagcatgagt
     2281 tattatttgg agcttgtcaa agtgacgtta agcttactaa tacgcaagaa catattttaa
     2341 tgttactatc tcagcaacgt ctcactaata cagatttggc taaggcatta aatattagtc
     2401 aggcggcagt aactaaggct atcaagagtt tggtcaaaca agacatgtta gcaggaacta
     2461 aggatacggt tgatgctagg gtgacttatt ttgaattaac cgagttagct aagcctattg
     2521 cgtcagaaca tacccatcat catgatgaaa ccttaaatgt ttacaaccgt ttattacaaa
     2581 aattctccgc gaaagaatta gagattgtag ataagtttgt aacagttttt gctgaggaat
     2641 tagaagggta atccatgaga tacatatcag tgaaaaatct ctcctttcaa tatgaaagtg
     2701 agccagtttt agaagggatc acttatcatt tagatagtgg agaatttgtc accatgaccg
     2761 gtgaaaatgg tgctgcaaag tcaaccttaa taaaagcaac cttaggaatt ttacaaccaa
     2821 aggctggacg agttactatt gctaaaaaaa ataaagacgg taaacaatta agaattgctt
     2881 acttgccgca gcaagtagct agctttaacg ctggttttcc atccaccgtt tacgagtttg
     2941 tcaaatcagg tcgctaccca cgtagtggtt ggtttagaca tttgaacaaa cacgatgaag
     3001 agcatgtgca agcaagctta gaagcagtcg gcatgtggga aaaccgtcat aagagaattg
     3061 gtagtttatc aggtggtcaa aaacaacgtg tggttattgc ccgtatgttt gcttctgacc
     3121 ctgatatttt tgtgctagac gagccaacaa cgggaatgga tagcggtact actgatacct
     3181 tttatgaact gatgcaccac agtgcacatc aacatgggaa atccgttctg atgattaccc
     3241 atgacccaga agaagtgaag gcttatgctg atcggaacat tcatttagtc agaaaccaaa
     3301 aacttccttg gcgttgtttc aacattcatg aagctgaaac agatgacgaa aaaggaggtc
     3361 atggtcatgc ttgatatttt attctatgat ttcatgcaac gggcggtaat ggcggtagtt
     3421 gccattagta tttttgctcc gattttaggt attttcctta ttttacgtcg tcaaagtttg
     3481 atgagcgata cccttagtca tgtttctttg gctggggtag cgcttggggt agtccttggt
     3541 atttcaccaa ccatcactac tattattgtt gtggttttag ctgctatttt gttagaatac
     3601 ctgcgtgtag tttacaaaca ctacatggag atttcaacgg cgattttgat gtcacttggc
     3661 ttggccctat ctctgattat tatgagtaag tcgcatagtt catcaagcat gagtttagaa
     3721 caataccttt ttggatcgat catcacgatt agtatggaac aagttgtcgc cttgtttgct
     3781 attgctgcga ttattttaat cttgaccgtt ctcttcatta gaccgatgta cattctgacc
     3841 tttgatgaag atactgcttt tgtagatggt ttgcccgttc gcttgatgtc tgttctattc
     3901 aatatcgtca ctggggttgc tattgctttg accattccag cagcaggagc acttttggtt
     3961 tctaccatta tggtcttgcc agcaagtatc gcaatgagat tgggtaaaaa ctttaaaaca
     4021 gttatcttac tgggaattgt catcggtttt agcggtatgt tatctggtat tttcttatct
     4081 tatttctttg aaacgccagc tagtgccact attaccatga ttttcattag tattttcctc
     4141 ttagttagtc taggtggaat gcttaaaaaa cggttatttt aataagaaaa atcctcacat
     4201 gtttaacatg tgaggattta tttataaatg ctttaaaaag ggggtaaggt cccaattttc
     4261 ttgattaatg gtgatgccct cccaaaagct atcattagtt aaacgaggaa tcaaatgaag
     4321 gtgaaaatga gtcccctcat ccatcaactc cttgtcattg ctgacgctag taacgcctga
     4381 aatgggaagg tgctgctcta gcttagcaac tagagcggct tgtaagtcga tgagatcata
     4441 gtgcccttcc tttggtagtt gtgtcagact agtgtaatgg tctttggaaa tcagtaaaag
     4501 gtgccccttt tggataggat cgatatcaaa aacaaccttg aaatattgac tttgagtcag
     4561 tagctgattt gctttgagct gatggcaaaa gatacattct gtcataggta catctcctta
     4621 tgaaaaaacg agccagagct cgtttaatcg gttatctagt aagtgagaac agcgtatttt
     4681 ttcttaccgc ggcgaataac ggttaattga tcatcaattt tatcatcatt acttaattgg
     4741 taatctaaat cttgaacgcg gtctccgtta atgtagattg ctccattttg cacgtcttca
     4801 cgcgcctggc gttttgatgg ggaaatctta gcagctacta aaatctcgac aatattatga
     4861 ttgtctattg attgaacgtg ataatttggt acattgctca atccttgttt taattcattg
     4921 gcagaaagat ttttgatgtt tccagcaaat aactgctcgg taatgttaag agcttgttta
     4981 taagcttctt cgccatgaac caaggtaacc acttcgcgtg ccaaggtttt ttgagcgaga
     5041 cgctcgtgac gagcggcatt aaattgagtt tcaatttctg caatctcatc tagagataag
     5101 aaggtgaaga ttttcaagaa acgcactgca tcatcatcca tgacatttaa ccagaattgg
     5161 tacatctcgt aaggagacgt cttatcggca tcaagccaga cagcattacc ttctgattta
     5221 ccgaattttt tcccggttga gtcagtgatg agtggtacag tcatgacgtg cccagtttta
     5281 tcagcctttt tgcggagcaa ttcagtacca gctgtcatat taccccactg gtcagagccg
     5341 ccaatttgta aggttacatt atgcttgtca ttgagttcgt agaagtcgta gccctgcatg
     5401 atttggtaag caaactcagt gtaagaaatg cctgtttcaa tgcgtttttt aacagagtct
     5461 ttactcatca tgtagttaac ggtgaagtat ttaccgacat cacggaggaa gtcaataaag
     5521 ctgatttgcg agaaccagtc gtagttgttg acaagctctg ctttattatc accattttca
     5581 aaatcaagga aagtagacaa ctgcccttta atcttgtcac tccactccaa aactgtttct
     5641 ttggtttgaa ggctgcgctc tgcatcttta aaggaaggat caccaattaa acctgtggca
     5701 ccccccacaa gagcgtaagg tttgtgccct gctagttgca agcgacgaga tgttaagata
     5761 gccactagat ggcccagatg aaggctgtca gcggttggat cataaccggt ataataggat
     5821 acttgccctt ctgttaatgc tttgacaagg gcttgttcat cagtcgtttg aaagaccaag
     5881 ccacgagctt tgagttcttc aaaaatattc ataggctttc tccattttca ttatagtttc
     5941 gaatcattat atcaaaaaac gctactgcta tgcaagcttt atcaaagttt ggtataatga
     6001 taaatgaggt attttatggt gaaatggaac acgaaacaaa agcgtataag tcatcaaaga
     6061 ttaggtctct tggatttggg gccggtgcta ttacgtacgt tgagactact gtctaacttt
     6121 ttttatattg ttatcttcct ttttggaatg atgggatttg gtatggcatt tgggtatttg
     6181 gctagtcaga ttgaatctgt taaggtacca agtaaagaaa gtttagtcaa acaagttgaa
     6241 tcattaacga tgatttcgca aatgaactat tctgataata gtttaatttc tactttagat
     6301 acggatttac ttcgaacacc agtagctaat gatgcgattt cagagaatat caaaaaagct
     6361 attgtatcaa cagaagacga acattttcaa gaacataaag gtatcgtgcc aaaagctgtt
     6421 tttcgggcaa cattggcttc tgtattggga tttggagaag ctagtggagg ttcgacctta
     6481 acacagcaat tagtcaagca acaagttttg ggagacgatc ccacatttaa gcgcaagtct
     6541 aaggagatcg tttacgctct tgccttagag cgttatatgt ccaaagacaa tatcttatgt
     6601 gattatctta atgtttcacc ttttggccgt aacaacaagg gtcaaaatat tgctggtgtt
     6661 gaagaagctg cgcgtggcat ttttggcgtt tctgccaaag atttaacggt gccacaggca
     6721 gcatttttgg cgggtcttcc gcagagtcct attgtttact ctccttattt gtcaacggga
     6781 caactgaaat cagaaaagga catggcttat ggcatcaagc gtcagcaaaa tgttctcttt
     6841 aacatgtacc gtacaggtgt tctgtctaaa aaagaatacg aggactataa ggcttatccg
     6901 attcaaaagg attttattca accgggaagt gcaatagtaa ataatcacga ttacctttat
     6961 tacacggtgt tagcggatgc taagaaagcc atgtatagct atttgattaa gcgagataag
     7021 gtgtctagtc gtgacttgaa aaatgacgag actaaggctg cttatgaaga gagagcctta
     7081 acagaattgc aacagggtgg ctataccatc accacaacca ttaataagcc tatttacaat
     7141 gcgatgcaga cagcggcagc tcagtttggt ggcttgttag atgatggcac tggtacagtt
     7201 caaatgggaa atgtcttgac agacaatgcg actggtgctg tgttaggttt tgttggtggt
     7261 agagattatg ctctgaatca aaataatcat gctttcaata cagttagatc gccaggttct
     7321 agcattaaac cgataatcgc ttatggtcct gctattgatc aaggtttaat ggggagtgct
     7381 agcgttttgt ctaattaccc aacaacttac tcgagtggcc aaaaaatcat gcatgctgat
     7441 agtgaaggaa cagccatgat gccacttcaa gaggccctaa atacttcttg gaacatccca
     7501 gctttttgga cacagaaatt actgcgtgaa aaaggtgtcg atgtcgaaaa ttatatgaca
     7561 aaaatgggtt ataagattgc agactactcg attgaaagtt tacctctagg gggcggtatt
     7621 gaagtctcgg ttgctcaaca aaccaatgct taccaaatgc tttcaaacaa tggcttatat
     7681 caaaagcaat atattgtaga taagattact gctagcgatg gtacagtcgt ttacaaacat
     7741 gaaaataagc caattcgtat tttttctgca gcaacagcta cgattttaca agaattgttg
     7801 agaggtccga ttacttcagg cgctacgact actttcaaga accgtttggc ggctattaat
     7861 ccgtggcttg ctaatgctga ttggattggt aagaccggaa caactgagaa ttatacggat
     7921 gtttggctag tcctgtctac tccaaaagtt actttaggcg gttgggcagg acatgatgac
     7981 aatacctcat tagcgccatt aacaggatat aacaataatt ctaattatct tgcctattta
     8041 gctaatgcca ttaatcaggc cgatcccaat gttattggag tagggcaacg cttcaactta
     8101 gatccaggag tcattaaggc gaatgtcttg aagtcaacag gtttacaacc aggaactgtt
     8161 aatgtcaatg gacatacttt ttctgttggt ggagaaatga ccaccagtct atggtcccaa
     8221 aaaggaccgg gggctatgac ttaccgattt gctattggtg gcacggatgc cgattatcaa
     8281 aaagcctggg ggaacttcgg gttcagaaaa aattaggttg ccatttatcg ataaaaaggg
     8341 tataatagta ataactattt gtcgtccaat ctagctgaaa tattgtccag ttaggaagaa
     8401 cagcagttaa atcacactga taaagtcaga tttagctgct ctttttgtgt ctatttttag
     8461 aaaagatagg gtttgtaacc tatatttaaa tattctaaaa attaacattt agggcaaatg
     8521 ataacttagt tgcgatttgc tgaaacagca aagcttaaat agaagaagga gctaaaaact
     8581 tggcaggaca tgaagttcga tacggaaaac accgtacacg tcgtagcttt tcaagaatca
     8641 aagaagttct tgatttacca aatttgattg aaattcaaac tgactcattc caagatttcc
     8701 tcgattcagg tttgaaagaa gtatttgaag atgtacttcc tatttcaaac tttacggata
     8761 ctatggaact tgaatttgtt ggttacgaat ttaaagaacc taaatatacc cttgaagaag
     8821 ctcgtatcca cgatgcaagt tattctgcac caatctttgt taccttccgt ttggtcaata
     8881 aagaaactgg tgagattaaa actcaagaag tcttctttgg tgatttccca atcatgacgg
     8941 aaatgggtac ctttattatc aatggtggtg aacgtatcat cgtttctcag ttggtgcgtt
     9001 ctccaggtgt ttatttcaac gataaagttg ataaaaacgg taaagttggt tacggatcaa
     9061 cagtgattcc taaccgtggg gcttggttgg aattagagac tgactcaaaa gacattgcct
     9121 acactcgtat tgaccgtacc cgtaagattc cattcacaac cttggttcgt gcccttggtt
     9181 tctcaggtga tgatgaaatt gttgatatct ttggtgaaag cgaccttgtt cgtaatacca
     9241 ttgaaaaaga cattcacaaa aatccaagtg attctcgtac agacgaagcc cttaaagaaa
     9301 tttacgagcg tcttcgtcca ggtgagccaa aaactgcaga tagctctcgt agtcttttga
     9361 ttgcgcgttt ctttgatgca cgtcgttacg atttggctgc ggttggtcgt tacaaagtga
     9421 acaaaaaact taatatcaag actcgtcttt tgaaccaaat cattgctgaa aaccttgtgg
     9481 atgctgaaac tggcgaaatt ttggtagaag ctggaactga gatgactcgt agcgtcatcg
     9541 aatccatcga agaacacctt gacggtgatt tgaacaagtt tgtttacaca ccaaatgatt
     9601 acgctgtggt tactgaacca gttgtccttc aaaagttcaa ggttgtgtca ccaattgatc
     9661 ctgatcgtgt agtaactatt gttggtaatg ccaatccaga tgataaggtt cgtgcattga
     9721 cacctgctga tattttggca gaaatgtctt acttcttgaa ccttgctgaa ggtcttggaa
     9781 aagttgacga tattgaccac ttgggtaacc gtcgtatccg tgccgttggt gaattgcttg
     9841 ccaaccaatt ccgcatcggt cttgctcgta tggagcgtaa cgtgcgtgag cgtatgtctg
     9901 ttcaagacaa cgatgtgtta acaccacaac aaatcatcaa tatccgtcct gtcacagcag
     9961 ctgtcaaaga attcttcggt tcgtctcagt tgtcacagtt catggaccaa cacaacccat
    10021 tgtcagagtt gtctcacaaa cgtcgtttat ctgccttagg acctggtggt ttgacacgtg
    10081 accgtgctgg ttatgaggtt cgtgacgtgc attacacgca ctatggccgt atgtgtccga
    10141 ttgaaacacc tgaaggacca aacattggtt tgattaataa cttgtcttca tttggacatc
    10201 ttaataaata tggtttcatc caaacacctt accgtaaggt tgaccgtgcg actggtacgg
    10261 taactaacga gattgtttgg ttgactgccg acgaagaaga cgaatacaca gttgcacagg
    10321 ccaattcgaa actaaacgaa gatggcactt ttgctgaaga aatcgttatg ggtcgtcacc
    10381 aaggtaataa ccaagaattt tctgcaagtg ttgttgattt cgttgacgtt tcccctaaac
    10441 aggtagttgc tgttgcgacg gcatgtattc ctttcttgga aaacgatgac tccaaccgtg
    10501 ccctcatggg tgccaacatg caacgtcagg ctgtgccatt gattgatcca aaagcaccat
    10561 atgttggtac tggtatggaa tatcaagctg cccatgactc aggcgctgcg gtgattgctc
    10621 agcaaaatgg taaagttgtc ttttctgatg ctgaaaaagt ggaaatccgt cgtcaagatg
    10681 gctcgcttga tgtttaccac attaccaaat tccgtcgttc aaactcagga acagcctata
    10741 accaacgcac ccttgttaaa gtaggagaca ttgttgaaaa aggtgatttc atcgctgatg
    10801 gaccttctat ggaaaatggt gaaatggctc ttggacaaaa cccagtcgtt gcttacatga
    10861 cttgggaagg ttataacttt gaggatgccg ttatcatgag tgagcgcctt gtgaaagaag
    10921 atgtctacac atctgttcac ttggaagaat tcgaatctga aacgcgtgat acaaaacttg
    10981 gccctgaaga aatcactcgg gaaatcccaa atgttggtga agaagccctc aaagaccttg
    11041 acgaaatggg cattatccgt atcggtgctg aggttaaaga aggcgacatc ttagtaggta
    11101 aagtcacacc taaaggtgaa aaagaccttt ctgctgaaga acgtttactt cacgccatct
    11161 ttggggataa atcgcgtgaa gtgcgtgata cctcacttcg tgtccctcac ggtggtgatg
    11221 gtatcgttcg tgatgtgaaa atctttacac gcgctaacgg cgatgaattg caatcaggtg
    11281 ttaatatgct tgtgcgtgtt tacatcgctc aaaaacgtaa aatcaaggtc ggagataaaa
    11341 tggccggtcg tcacggaaac aagggtgtcg tttcacgtat tgtaccagtt gaagacatgc
    11401 cataccttcc agatggaaca ccagttgaca tcatgttgaa ccctcttggg gtgccatcac
    11461 ggatgaatat tggtcaggtt atggaacttc accttggtat ggctgctcgt aatcttggta
    11521 ttcacattgc aacacctgtc tttgacgggg cttcatcaga agacctttgg gacactgttc
    11581 gtgaagctgg tatggatagc gatgctaaga cggtccttta tgatggtcgc accggtgaac
    11641 catttgacaa ccgtgtatcc gttggtgtca tgtatatgat caaacttcac cacatggttg
    11701 atgataaact tcatgcccgt tctgtaggac catactcact tgttacccaa cagccacttg
    11761 gtggtaaagc tcaatttggt ggacaacgtt ttggtgagat ggaggtttgg gcccttgaag
    11821 cttatggtgc atcaaatgtt cttcaagaaa tcttgaccta caagtcagat gacgtcacag
    11881 gacgtttgaa agcctatgaa gcgattacta aaggtaaacc aattccaaaa ccaggtgtac
    11941 cagaatcctt ccgtgttctt gtaaaagaat tgcaatcgct tggtcttgat atgcgtgtgc
    12001 ttgacgagga tgataatgaa gtggaacttc gtgatcttga tgaaggtgaa gacgatgaca
    12061 ttatgcatgt tgacgatctc gagaaggcac gtgaaaaaca agctcaagaa actcaagaag
    12121 tttctgaaac aactgacgaa aaataagcaa tcaattctta ttaaataatt atttactggt
    12181 ctggggcaaa ggccccagga actggtaaag tcatcaaagg cagaaaggta aaactagtgg
    12241 ttgacgtaaa tcgttttaaa agtatgcaaa tcacattagc ctcaccaagt aaggtccgtt
    12301 catggtctta tggtgaagtt aaaaaacctg aaacaatcaa ctaccgtaca ttaaaaccag
    12361 aacgtgaagg actctttgac gaagtcatct ttggtccaac aaaagactgg gaatgtgcgt
    12421 gtggtaagta caaacgtatc cgttataaag ggatcgtttg tgatcgctgt ggggttgagg
    12481 taactcgtgc taaagtacgt cgtgaacgta tgggtcacat tgagttaaaa gctcctgtat
    12541 cacatatctg gtatttcaaa gggattccat ctcgtatggg attgactctt gatatgagtc
    12601 ctcgtgccct tgaagaagtg atctattttg cggcttatgt ggtcattgat ccaaaagata
    12661 caccgcttga accaaaatca ttattaacag aacgtgaata ccgtgaaaaa ctacaagagt
    12721 atggtcatgg gtcatttgtc gctaagatgg gtgctgaagc cattcaagat ctcttgaaac
    12781 gtgtggactt ggcagctgaa atcgctgaat taaaagaaga gttgaaatct gcttctggtc
    12841 aaaaacggat taaagcagtt cgtcgtttag acgtccttga tgcctttaac aaatctggaa
    12901 ataaaccaga atggatggtt cttaacatcc tgccggttat tccaccagat cttcgtccga
    12961 tggttcaatt ggatggtggt cgttttgcgg catcagactt gaatgacttg taccgtcgtg
    13021 tgattaaccg taataaccgt ttggcacgtt tgttagaact taatgcccct ggcatcattg
    13081 ttcaaaatga aaaacgaatg cttcaagaag ccgttgatgc tttgattgat aatggtcgtc
    13141 gtggtcgtcc aatcactgga ccaggaagtc gtccattgaa atcattgagc cacatgctta
    13201 aaggtaaaca agggcgtttc cgtcaaaact tgcttggtaa acgtgtagac ttctctggac
    13261 gttccgttat cgctgttggt ccaaccctta aaatgtatca atgtggtgtc ccacgtgaaa
    13321 tggctatcga gctttttaaa ccatttgtaa tgcgcgaaat tgttgccaaa gaatatgctg
    13381 gtaacgttaa agccgctaaa cgtatggttg aacgtggcga cgaacgcatc tgggatatct
    13441 tagaagaagt tatcaaagaa cacccagtcc tacttaaccg cgcaccgact cttcacagac
    13501 ttggtattca ggcttttgaa cccgttctta ttgacggtaa ggcacttcgt cttcacccac
    13561 ttgtgtgtga ggcctacaat gccgacttcg atggagacca aatggccatt cacgtgccac
    13621 tttcagaaga agcacaagct gaagctcgtc ttttgatgct tgctgcagag cacatcttga
    13681 accctaaaga tggtaaacca gttgttactc catctcagga tatggttctt ggtaactact
    13741 atcttacgat ggaagatgct ggtcgcgaag gcgaaggtat gattttcaag gataaagacg
    13801 aagctgtgat ggcatatcgt aatggttatg ctcatcttca tagtcgtgtg ggtatcgctg
    13861 ttgacagcat gccaaacaaa ccttggaaag acaatcaaag acataaaatc atggtgacaa
    13921 ctgttggtaa gattctcttt aacgatatca tgccagagga ccttccttac ctccaagagc
    13981 caaacaatgc caacttgaca gaaggaacac ctgataaata cttccttgaa cctggtcaag
    14041 acatccaaga agtgattgat cgcttagaca tcaatgtgcc atttaagaag aaaaacctcg
    14101 gtaacatcat tgcggaaacc ttcaaacgtt tccgtacaac agaaacatca gccttccttg
    14161 accgcttgaa agaccttggt tactaccact caacccttgc tggtttgaca gtgggtatcg
    14221 ctgacattcc tgttattgat aataaagctg aaatcattga tgctgctcac catcgtgttg
    14281 aagaaattaa caaagccttc cgtcgtggtt tgatgacaga tgatgaccgt tatgttgccg
    14341 ttacaacaac atggcgtgaa gctaaagaag cccttgaaaa acgtctgatt gaaacacaag
    14401 atcctaagaa cccaatcgtt atgatgatgg actcaggagc tcgtggtaac atctcaaact
    14461 tctcacagct tgctggtatg cgtggtttga tggctgctcc taacggacgc atcatggaac
    14521 ttcctatctt gtcaaacttc cgtgaaggtt tgagcgtttt ggaaatgttc ttctcaaccc
    14581 acggtgcacg taaagggatg accgatacgg cccttaaaac agccgactca ggttacctta
    14641 ctcgtcgttt ggttgacgtt gcccaagatg ttatcattcg tgaggacgat tgtggcactg
    14701 atcgtggtct tcttatccgt gctattacag atggtaaaga agttaccgaa acgcttgaag
    14761 agcgtcttca aggtcgttac acacgtaaat cagtcaaaca ccctgaaact ggtgaagtct
    14821 tgattggtgc tgaccaatta atcactgaag acatggctcg taagattgtt gatgcaggtg
    14881 ttgaagaagt gaccattcgt tctgtcttta cctgtgcgac tcgtcatggt gtctgccgtc
    14941 actgttatgg tatcaacttg gcaactggtg atgctgttga agtgggtgaa gcagttggta
    15001 ctattgctgc ccaatctatc ggtgagcctg gtactcagct taccatgcgt accttccaca
    15061 cgggtggtgt agcctcaaat accgatatca cccagggtct tcctcgtatt caagagatct
    15121 ttgaagcacg taatcctaaa ggggaagcgg tcattactga agtgaaaggg aatgtcgtcg
    15181 agattgaaga agatgcgtca actcgtacca agaaagtcta cgttcaagga aaaactggca
    15241 tgggcgaata tgtcatacca tttacagcac gtatgaaagt tgaagttggc gacgaagtta
    15301 atcgcggagc tgcccttaca gaagggtcaa ttcaaccgaa acgtctcctt gaagtgcgtg
    15361 ataccttgtc agttgaaacg taccttcttg cagaagtaca aaaagtttac cgtagccaag
    15421 gggtagaaat cggagacaaa cacgttgagg taatggttcg ccaaatgctt cgtaaagttc
    15481 gtgtcatgga tccaggtgat acagacctcc ttccaggtac acttatggat atttctgatt
    15541 tcacagatgc taacaaagat attgttatct ctggtggtat tcctgcgaca tctcgtcctg
    15601 ttcttatggg tattactaag gcttcccttg aaaccaattc cttcttatca gctgcatcct
    15661 tccaagaaac aactcgtgtt cttacagatg ctgctatccg tggtaaaaaa gatcatcttc
    15721 ttggtcttaa agaaaatgtt atcattggta aaatcatccc agctggtact ggtatggctc
    15781 gttaccgtaa cattgaacca caagcgatga atgagattga agtgattgat catacagaag
    15841 tctcagcaga agccgttttt acagcagaag cagaataatc tcatgaaagg gtccttaggg
    15901 gctctttttt gtctgagttt gttggcagta aaaagcattg acaatcagtc ttagtcccaa
    15961 tgagcagatg tttttcaaaa tacttatgat ttagctataa taatgcataa gaggaatggg
    16021 taatgtgtta tgtatcaagt aattaaaatg tatggtgatt gggagccttg gtggttcatc
    16081 gatggatggc aagacgatat tatagatgag caacaattta gtgactggca agaagcgctt
    16141 gattatttta atcaagaatg gcagcgcatg aaagctattt ttcctagtta tcatagtcaa
    16201 aagaatttgt tagctacttt ttgggaaaaa gaagataaaa gatggtgcga ggactgtgac
    16261 gaagatttgc agcagtttca ttctctttta ctccttaaaa ataaagatat tgtaccaagc
    16321 aataattata tccctgaatt tgaacaacga aatgattcac cacaggtagc ttatctttgc
    16381 aagttaaacc tttaagtgtt tgttaaaaac aaaa
//
LOCUS       DAXWIQ010000037        15829 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000037 DAXWIQ010000000
VERSION     DAXWIQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15829
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.037"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            65..241
                     /gene="rpsU"
                     /locus_tag="ACGEUI_001317"
     CDS             65..241
                     /gene="rpsU"
                     /locus_tag="ACGEUI_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFF8166147.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
     gene            complement(369..731)
                     /gene="mscL"
                     /locus_tag="ACGEUI_001318"
     CDS             complement(369..731)
                     /gene="mscL"
                     /locus_tag="ACGEUI_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFF8166148.1"
                     /translation="MVKELKAFLFRGNIIELAVAVIIGGAFGAIVTSFVNDIITPLIL
                     NPALKAANVENITQLSWNGVKYGSFLGAVINFLIIGTSLFFVVKAAEKAMPKKEKEAA
                     APTQEELLTEIRDLLAQK"
     gene            862..2676
                     /gene="dnaG"
                     /locus_tag="ACGEUI_001319"
     CDS             862..2676
                     /gene="dnaG"
                     /locus_tag="ACGEUI_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFF8166149.1"
                     /translation="MGFLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYL
                     GLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGM
                     SLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQ
                     LIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFHLSDQSNTIYDAFRNRIMFPLS
                     DDRGHIIAFSGRIWTAADMEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVF
                     LMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAK
                     SLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVD
                     NLQSQIVYVEKMAPLIAQSPSITAQHSYINKISDLLPNFDYFQVEQSVNALRIQDRQK
                     HQGQIAQAVSNLVTLPMPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTL
                     ELLYQRLKQQGHITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSKRAKLLA
                     ERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME"
     gene            2685..3794
                     /gene="rpoD"
                     /locus_tag="ACGEUI_001320"
     CDS             2685..3794
                     /gene="rpoD"
                     /locus_tag="ACGEUI_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFF8166150.1"
                     /translation="MTKQKEITTFNVQVAEFIRHHKKEGTAIDDDVTEKLVIPFALDA
                     DQIDDLLERLTDGGISITDKEGNPSSKYIVEEPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTSEEEKELAVAVAKGDLMAKQRLTEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMEMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLRDFIED"
     gene            4030..4368
                     /locus_tag="ACGEUI_001321"
     CDS             4030..4368
                     /locus_tag="ACGEUI_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFF8166151.1"
                     /translation="MSDTPKYTQDQVIAIKNRILEALETVIDPELGIDVVNLGLIYEI
                     RFNDNGYTEIDMTLTTMGCPLADLLTDYIHDALQDVPEVTKTEVKLVWYPAWTVDKMS
                     RYARIALGIR"
     gene            4506..5360
                     /gene="rfbD"
                     /locus_tag="ACGEUI_001322"
     CDS             4506..5360
                     /gene="rfbD"
                     /locus_tag="ACGEUI_001322"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFF8166152.1"
                     /translation="MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEA
                     VFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTD
                     YVFDGNKPVGQEWVETDHPDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKN
                     FVFTMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDT
                     TWYDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKA
                     FYQQGLKK"
     gene            5479..6633
                     /gene="cps2T"
                     /locus_tag="ACGEUI_001323"
                     /gene_synonym="wchF"
     CDS             5479..6633
                     /gene="cps2T"
                     /locus_tag="ACGEUI_001323"
                     /gene_synonym="wchF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta 1-4 rhamnosyltransferase Cps2T"
                     /protein_id="HFF8166153.1"
                     /translation="MQDVFIIGSRGLPAKYGGFETFVEELISHQSSKNIRYHVACLSD
                     TKHKVHFDYKGADCFYLNPPKLGPARVIAYDMMAITYALSYSDQHQIQNPIFYVLGNT
                     VGAFIAPFVKQIHNRGGRFFINPDGLEWKRSKWSRPVQAYLKFSEKQMTRQADLVISD
                     NIGIDRYLKQVYPWSKTCFIAYGTQTQPSRLATADSKVRAYFQTFDIREKDYYLILGR
                     FVPENNYETAIKEFMASSTKRDLVIICNHEGNAYFKQLLAETECDKDPRIKFVGTLYD
                     KELLAYIREQAYAYIHGHEVGGTNPGLLEALAHTNLNLVLGVDFNQSVAKSAALYWTK
                     QKGQLAELINQVDAGFDSDHLGKEAKAIIQEHYTWEKIVGEYEALFLNEH"
     gene            6623..7555
                     /locus_tag="ACGEUI_001324"
     CDS             6623..7555
                     /locus_tag="ACGEUI_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8166154.1"
                     /translation="MNINILLSTYNGERFLAEQIQSIQRQTVNDWTLLIRDDGSTDGT
                     QDIIRTFVKEDKRIQWINEGQTENLGVIKNFYTLLKHQKADVYFFSDQDDIWLDNKLE
                     VTLLEAQKHEMTAPLLVYTDLKVVTQHLAVCHDSMIKTQSGHANTSLLQELTENTVTG
                     GTMMITHALAEEWTTCDGLLMHDWYLALLASAIGKLVYLDIPTELYRQHDANVLGART
                     WSKRMKNWLTPHHLVNKYWWLITSSQKQAQLLLDLPLKPNDHELVTAYVSLLDMPFTK
                     RLATLKRYGFRKNRIFHTFIFRSLVVTLFGYRRK"
     gene            7558..8361
                     /locus_tag="ACGEUI_001325"
     CDS             7558..8361
                     /locus_tag="ACGEUI_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8166155.1"
                     /translation="MNFLTKKNRILLREMVKTDFKLRYQGSAIGYLWSILKPLMMFTI
                     MYLVFIRFLRLGGNVPHFPVALLLANVIWSFFSEATSMGMVSIVSRGDLLRKLNFSKH
                     IIVFSAVLGALINFLINLVVVLIFALINGVTISGYAYLSLFLFIELVVLVLGIALLLS
                     NVFVYYRDLAQVWEVLLQAGMYATPIIYPITFVLDSHPLAAKLLMLNPVAQMIQDFRY
                     LLIDRANVTIWQMSTNWFYIVIPYLVPFVILFIGIFVFKKNADRFAEII"
     gene            8361..9566
                     /locus_tag="ACGEUI_001326"
     CDS             8361..9566
                     /locus_tag="ACGEUI_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8166156.1"
                     /translation="MINREIAVKVQHVSKTFKLPTEATKSFRTTLVNRLRGIKGFTEQ
                     HVLKDINFDVYKGDFFGIVGRNGSGKSTLLKIISQIYVPEKGQVTVDGKMVSFIELGV
                     GFNPELTGRENVYMNGAMLGFTKDEVDDMYNDIVDFAELHHFMNQKLKNYSSGMQVRL
                     AFSVAIKAQGDVLILDEVLAVGDEAFQRKCNDYFMERKDSGKTTILVTHDMGAVKKYC
                     NRAVLIEDGLVKAYGEPFDVANQYSVDNTETAEDAMNAEKISVSDIAKDLKVSLISNP
                     RITPNDTITFEVSYEVLKDDFTYIAFSLTDIDRNIWVYNDNSLDYPTQGIGRKCISYQ
                     CQLSQLNDIKLKLEVTVRDKNGQMLLFSTAEQSPQIVIQRNDIDQDDLSALDSASGLY
                     QRNGKWVFH"
     gene            9591..10598
                     /locus_tag="ACGEUI_001327"
     CDS             9591..10598
                     /locus_tag="ACGEUI_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8166157.1"
                     /translation="MYKVSIICTNYNKAPWISDALDSFLSQVTDFEVEIIVIDDASTD
                     DSREILKSYQKKSSGKIKLLFNETNIGITKTWIKACLYAKGKYIARCDGDDYWTDSFK
                     LQKQVDVLEASKRSRWCNTDFDFVNSQGDLLYANAFSSGHTPLTDTYEKVLALKGMTM
                     ASTWLVDADLMRQVNQKINVDTPDDTFDIQLELFQLTQLTYIKDSTTIYRMTTDSDSR
                     PTDTPKMIYRIQKLLDTQLNYLKKYNQVDTKEVSELLLQQDAKQEIRIHELSCFIQEL
                     QQTIVDKTKQQETREVELQNVIEEQKNQLSELRQQYHAIINSRQWKYTSKLIAFIRRK
                     K"
     gene            10595..12340
                     /locus_tag="ACGEUI_001328"
     CDS             10595..12340
                     /locus_tag="ACGEUI_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HFF8166158.1"
                     /translation="MNRILLYVHFNKYNKISAHVYYQLEQMRSLFSKIVFISNSKVSH
                     EDLKRLKNHCLIDEFLQRKNKGFDFSAWHDGLIIMGFDKLEEFDSLTIMNDTCFGPIW
                     EMAPYFENFEEKETVDFWGITNNRGTKAFKEHVQSYFMTFKNQVIQNKVFQQFWQSII
                     EYENVQEVIQHYETQLTSILLNEGFSYQTVFDTRKAESSFMPHPDFSYYNPTAILKHH
                     VPFIKVKAIDANQHIAPYLLNLIRETTNYPIDLIVSHMSQISLPDTKYLLSQKYLNCQ
                     RLAKQTCQKVAVHLHVFYVDLLDEFLTAFENWNFHYDLFITTDSDIKRKEIKEILQRK
                     GKTADIRVTGNRGRDIYPMLLLKDKLSQYDYIGHFHTKKSKEADFWAGESWRKELIDM
                     LVKPADSILSAFETDDIGIIIADIPSFFRFNKIVNAWNEHLIAQEMMSLWRKMDVKKQ
                     IDFQAMDTFVMSYGTFVWFKYDALKSLFDLELTQNDIPSEPLPQNSILHAIERLLVYI
                     AWGDSYDFRIVKNPYELTPFIDNKLLNLREDEGAHTYVNFNQMGGIKGALKYIIVGPA
                     KAMKYIFLRLMEKLK"
     gene            12337..14811
                     /locus_tag="ACGEUI_001329"
     CDS             12337..14811
                     /locus_tag="ACGEUI_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFF8166159.1"
                     /translation="MIKDTFLKTNWLNISHHIILLVFGFYFSFYSLAKELVSSTAQPV
                     NYYAHLLNVSFVGYIISLIGLSYYLSRQVSRQLFLKTSFIVISYLIVSYWVQITQHLN
                     DKRFDIWSLTKNQFYQFQALPSLLIILVMATLIKILVAYFAIEKDRFGLLGYQGNTFS
                     VALILAVVPINDIHLLKLISSRFSELVTAGNSQIALLKISGLLIVLLVIFATIIYVVL
                     NALKHLKSNKPSFSVAATTSLFLALVFNYTFQYGVKGDEALLGYYVFPGATLFQIVAI
                     TLVALLAYVITNRYWPTTFFLLIPGTIISVVNDLKESMRSEPLLVTDFVWLQELGLVT
                     SFVKKSVIVEMVVGLAICIVVAWYLHGRVLAGKLFMSPVKRASAVLGLFIVSCSMLIP
                     FSYEKEGKILSGLPIISALNNDNDINWLGFSTNARYKSLAYVWTRQVTKKIMEKPTNY
                     SQETIASIAQKYQKLAEDINKDRKNNIADQTVIYLLSESLSDPDRVSNVTVSHDVLPN
                     IKAIKNSTTAGLMQSDSYGGGTANMEFQTLTSLPFYNFSSSVSVLYSEVFPKMAKPHT
                     ISEFYQGKNRIAMHPASANNFNRKTVYSNLGFSKFLALSGSKDKFKNIENVGLLTSDK
                     TVYNNILSLINPSESQFFSVITMQNHIPWSSDYPEEIVAEGKNFTEEENHNLTSYARL
                     LSFTDKETRAFLEKLTQINKPITVVFYGDHLPGLYPDSAFNKHIENKYLTDYFIWSNG
                     TNEKKNHPLINSSDFTAALFEHTDSKVSPYYALLTEVLNKASVDKSPDSPEVKAIQND
                     LKNIQYDVTIGKGYLLKHKTFFKISR"
     gene            14990..15685
                     /locus_tag="ACGEUI_001330"
     CDS             14990..15685
                     /locus_tag="ACGEUI_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8166160.1"
                     /translation="MKKLIIIPAYNESSNIVNTIRTIESDAPDFDYIIIDDCSTDNTL
                     AICQKQGFNVISLPINLGIGGAVQTGYRYAQRCGYDVAVQVDGDGQHNPCYLEKMVEV
                     LVQSSVNMVIGSRFITKEGFQSSFARRIGIKYFTWLIALLTGKKITDATSGLRLIDRS
                     LIERFANHYPDDYPEPETVVDVLVSHFKVKEIPVVMNERQGGVSSISLTKSVYYMIKV
                     TLAILVVRLKGNR"
     gene            15687..>15829
                     /locus_tag="ACGEUI_001331"
     CDS             15687..>15829
                     /locus_tag="ACGEUI_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HFF8166161.1"
                     /translation="MTVTASLFISFIVLTFVFFLINLIKKDKLAIKYSLLWFILALLI
                     LLFT"
BASE COUNT         5255 a         2562 c         3023 g         4989 t
ORIGIN      
        1 aaaaaaataa tgtaatataa ttgggaacgt gaaaatgtta caatatcttg aggaggtgaa
       61 acacatgtca aaaacagtag tacgtaagaa tgaatctctt gacgatgctc ttcgtcgctt
      121 caaacgttct gttactaaag ctggtactct tcaagaatca cgtaaacgtg aattctacga
      181 aaaaccttct gtaaaacgta aacgtaaatc agaagcagct cgcaaacgta aaaaattcta
      241 atggataaaa aataaaacag ttctcacaat tagaactgtt tttatttttt atccagaaga
      301 agcaagggag cttttttgat atcaaaaaaa gtagttagac atgcctaact actttatcaa
      361 aaatcgcttt atttttgagc caacaaatca cggatttctg tcaataattc ttcttgagta
      421 ggagctgcag cttctttttc ctttttaggc atagcttttt cagcggcttt tacaacaaag
      481 aaaagactgg taccgatgat aagaaaatta ataacggctc ctaaaaagct accatattta
      541 acaccattcc atgacaattg agtgatgttt tcaacatttg cagctttcaa agctgggttt
      601 aaaataagcg gtgtgataat atcgttcaca aaagaagtga caatagcacc aaaagctccc
      661 ccgatgataa ctgctacagc aagttcaata atgtttcctc taaacaaaaa tgcttttaat
      721 tctttaacca ttaaacattc ccccttttta atatctctaa gttaagcata gcataattag
      781 taaacaaaat ccaatggaat cacttcagtt tttaaattta caaaaaagag tttcggtggt
      841 ataataaaac gtaaaactaa aatgggattt ttatggggag gtgacgattt ggcaattgac
      901 aaagaaatga tttcccaagt aaaaaatagc gttaatattg tcgatgtcat tggagaagtg
      961 gtcaaacttt cccgatcagg gcggcattat ctcgggcttt gcccatttca taaggaaaag
     1021 acaccctctt ttaatgttgt tgaagacaga caattttttc actgctttgg ctgtggaaaa
     1081 tcaggggatg tttttaaatt tattgaggaa taccgccaag tccccttctt agaaagtgtt
     1141 cagattattg ccgataagac tggtatgtcg cttaatatac cgccaagtca ggcagtactt
     1201 gctagccaac acaagcaccc taatcacgct ttgatgacac ttcatgagga tgctgctaaa
     1261 ttttaccatg cagttttgat gaccactacc attggtcaag aagctaggaa gtacctttac
     1321 cagagaggct tggatgacca attaattgag catttcaata ttggtttagc cccagatgag
     1381 tcagattatc tttatcaagc tctttctaaa aaatacgagg aaggtcaatt ggttgcttca
     1441 ggattgtttc acttgtccga tcaatccaat accatttacg acgcctttcg aaatcgtatc
     1501 atgtttccct tatcagatga ccgagggcat attattgcct tttcaggacg tatctggacg
     1561 gcagctgata tggaaaagag acaggcaaag tataaaaatt caagaggaac agttcttttt
     1621 aacaaatctt atgaattgta tcatctggac aaggcaaggc ctgttattgc caaaacccat
     1681 gaagtgtttc taatggaagg gtttatggac gtgattgccg cttaccgttc cggctatgaa
     1741 aatgctgttg cttcaatggg gacggcattg actcaagaac atgtcaatca ccttaagcaa
     1801 gtcactaaaa aagttgtttt gatttatgat ggtgacgatg ctggacaaca tgctattgca
     1861 aaatcactag aattgcttaa agattttgtt gtcgaaattg tcagaatccc caataaaatg
     1921 gatcctgacg aatttgtaca acggcattcc ccagaagcat ttgcagattt gcttaagcag
     1981 tcacggatca gtagtgttga attttttatt gattacctaa aacctactaa tgtagacaat
     2041 ttgcaatcac aaattgttta tgtggagaaa atggcaccat tgattgctca atcaccatcc
     2101 atcacagctc aacattcgta tattaacaag atttctgatt tgttgccaaa ctttgactat
     2161 tttcaagtag aacaatcagt aaatgcatta aggattcaag ataggcaaaa acatcaaggt
     2221 caaatagctc aagccgtcag caatcttgtg accttaccaa tgccaaaaag tttgacagct
     2281 attgctaaga cagaaagtca tctcatgcat cggctcttac atcatgacta tttattaaat
     2341 gaatttcgac atcgtgatga tttttatttt gatacctcta ccttagaatt actttatcaa
     2401 cggctgaagc aacaaggaca cattacatct tatgatttgt cagagatgtc agaggaagtt
     2461 aaccgtgctt attacaatgt tttagaagaa aaccttccca aagaagtagc tcttggtgag
     2521 attgatgata ttttatccaa acgtgccaaa cttttagcag agcgcgatct tcacaaacaa
     2581 gggaaaaaag ttagagaatc tagtaacaaa ggcgatcatc aagcggctct agaagtacta
     2641 gaacatttta ttgcgcagaa acgaaaaatg gaatagagga aattatgaca aaacaaaaag
     2701 aaataacaac ttttaacgtt caagttgcgg agtttattcg ccatcacaaa aaagaaggaa
     2761 cagcgattga tgatgacgtg accgaaaaac ttgtcattcc atttgctttg gatgctgatc
     2821 agattgatga cttacttgag cggttaacag atggtggtat ctcgattacg gataaagaag
     2881 gtaacccatc atcaaaatac attgttgaag aaccaaaacc agaagaatta actgatgaag
     2941 aattaattgg tagtaattca gctaaagtaa acgaccctgt ccgcatgtac ctcaaagaaa
     3001 ttggtgtggt accgctttta acaagtgaag aggaaaaaga attggctgtt gcggttgcca
     3061 agggagattt aatggctaaa caacgtttaa cggaagcaaa ccttcgtctt gttgtctcaa
     3121 ttgcaaaacg ttatgttgga cgtggaatgc aatttcttga tttaattcaa gaaggtaaca
     3181 tgggactcat gaaagctgtt gataaatttg attattcaaa aggattcaaa ttctcaactt
     3241 atgcaacatg gtggattcgt caagctataa cacgcgcaat tgcagatcaa gcacgtacga
     3301 ttcgtatccc tgttcatatg gttgaaacca ttaataaatt ggttcgtgaa caacgtaacc
     3361 ttcttcaaga acttggtcaa gatccaacgc cagaacaaat cgcagaacgt atggaaatga
     3421 ctccagacaa ggttcgtgaa attctaaaaa ttgctcaaga acctgtttct ttagaaacac
     3481 caattgggga agaagatgat agtcacttag gtgattttat cgaagatgaa gttattgaaa
     3541 atccagtaga ttatacgacg cgtgttgttc ttcgtgagca attggatgaa gttttagata
     3601 cgcttactga ccgtgaagag aacgttcttc gcctacgctt tggccttgac gatggtaaaa
     3661 tgcgtacgtt agaggacgta ggtaaagtct ttaatgttac tcgtgagcgc attcgacaga
     3721 ttgaagctaa agcccttaga aaacttcgcc atcctagtcg tagtaaacaa ttaagagact
     3781 ttatagagga ctaaaatcgt ccagtggacg attttaggtc gttcctttga aataagaaag
     3841 gagcgaaaat cctaagtggc acttatcagc ggtacaccta caaattagaa atcggaccga
     3901 atgtggtggg tatttttaag ggtagacttg atatcttaaa caagcccaga aggcaattcc
     3961 tctaaatagg caaagcttta gtggtttttt tacttatcta cgacatattc aaaaaatgaa
     4021 aggaaagcca tgtctgatac accaaaatat acacaagatc aggtgatagc tatcaagaat
     4081 agaattcttg aagctttaga aactgttatt gatcccgagc taggtattga tgttgttaat
     4141 ctaggcttga tttatgaaat tcggtttaat gataacggtt atacagagat tgatatgaca
     4201 ctcacaacaa tgggttgtcc tttggcagac ttgttgacag attacatcca tgatgccctg
     4261 caagatgtgc cagaagtcac taagactgag gtcaaattag tctggtaccc tgcttggacg
     4321 gttgataaaa tgagccgcta tgctaggatt gcgctgggta ttcgctaatc attgatagta
     4381 aagggatgaa atattccctt ttttgatgta gaataaaagg aaatacaggt gaaagccttt
     4441 ggttaatgtt agagaacact aagatactga gaaaagtgtt ctacctatgg caaaaaggag
     4501 aactgatgat tttaattaca ggaagcaatg gtcaattagg gacagaactt cgatacttat
     4561 tagatgaacg aggtgttgat tatgtagccg ttgatgtcgc agaaatggac atcacaaatg
     4621 aagacaaagt cgaagctgtt tttgcgcagg tcaagccaac attggtctat cattgtgctg
     4681 cttatacggc agttgatgca gcagaagacg aaggaaaagc cttgaatgag gctattaatg
     4741 taacaggctc agaaaatatt gccaaggctt gtggaaaata tggggctacc cttgtttaca
     4801 tttcaacaga ctacgtattt gatggcaata aaccagtcgg tcaagaatgg gtagaaacag
     4861 atcatccaga ccctaagaca gaatatggcc gtacaaaacg tttaggagaa ctagctgttg
     4921 aacgatacgc agagcatttt tatattatcc gtactgcttg ggtatttgga aattatggta
     4981 aaaactttgt ctttaccatg gagcagttag cagaaaatca ctcccgtttg actgttgtca
     5041 atgaccaaca cggtcggcca acgtggacaa ggaccttggc agaattcatg tgttatttga
     5101 cagagaatca aaaagccttt ggttactatc acttgtcaaa tgatgctaag gaagacacca
     5161 cttggtatga ttttgccaaa gaaatcttaa aagacaaggc tgttgaagtg gtgccagttg
     5221 attcatcagc tttcccagca aaagccaaac gtcctttaaa ttcaaccatg aatcttgata
     5281 aggcaaaagc aacagggttt gttattccaa catggcaaga agccttgaag gcattttacc
     5341 aacaaggact gaaaaagtaa gtattctaaa aagagttggc tttctaaggc caactctttt
     5401 tcatgggttt aaggtcgcat tagccttcta attgtggtat aatatcactg attatatttt
     5461 agaatggggt agtatgcaat gcaggatgtt tttatcattg gtagcagagg gttaccagcg
     5521 aaatacggtg gctttgaaac ctttgtcgaa gagttgatta gtcatcaatc atctaaaaac
     5581 attcgttatc atgttgcttg tttaagcgat acgaagcata aggttcattt tgattataag
     5641 ggggcagatt gcttttacct taatccccca aaacttggtc ctgctagagt tattgcttat
     5701 gatatgatgg caattaccta tgccctgtct tatagcgacc aacaccagat tcagaaccct
     5761 attttttatg tgttgggcaa tactgtaggc gcttttattg cgccatttgt gaagcagatt
     5821 cacaatcgag gtggacgctt ttttatcaat ccagacgggt tagagtggaa acggtctaaa
     5881 tggtcaagac cagtgcaggc ctacctgaag ttttccgaaa agcaaatgac tagacaggct
     5941 gatttggtca tttcagataa tattggcatt gaccgctacc ttaaacaagt ttatccttgg
     6001 tctaaaacat gttttattgc ttatggaacg cagacacaac cttcacgttt ggcaacagca
     6061 gatagcaagg tcagagctta ttttcaaacc tttgatatta gagaaaagga ttactatctc
     6121 attttaggac gctttgttcc tgaaaataat tacgaaaccg ctattaaaga atttatggct
     6181 tcctcaacta agcgtgattt agtgattatt tgtaatcatg aaggcaatgc ttactttaag
     6241 caattacttg ctgagacaga gtgtgataaa gacccacgca ttaaatttgt tggtaccctt
     6301 tatgacaaag aactattagc ctacattcga gaacaggctt atgcctatat ccatggtcat
     6361 gaggtaggag ggactaatcc aggccttcta gaagcactag cccataccaa tcttaatttg
     6421 gttttaggag ttgattttaa ccagtcggtg gctaagtctg ctgcgcttta ttggaccaag
     6481 caaaaagggc aattagcaga gctgattaac caagttgatg caggttttga tagtgatcat
     6541 ctgggcaaag aagccaaagc tattattcaa gaacattaca cttgggaaaa aattgtaggg
     6601 gagtacgagg ctttattttt aaatgaacat taatatttta ctatccacct acaatggaga
     6661 acgcttctta gcagaacaaa ttcaaagtat ccaaaggcaa acagttaacg actggacctt
     6721 attgattaga gatgatggtt caactgatgg cacccaggat attattcgca catttgtcaa
     6781 ggaagacaaa cgaattcagt ggatcaatga ggggcagaca gaaaatctgg gagttattaa
     6841 gaatttttac accctcttaa agcaccaaaa agcagatgtc tattttttca gtgaccaaga
     6901 tgatatatgg ttagacaata aactggaagt gaccttattg gaggctcaaa aacatgaaat
     6961 gacagctcct ttattggttt atacagacct caaagtggta acccaacatt tagctgtttg
     7021 ccatgacagt atgattaaga cccaatctgg tcatgcaaac acaagcctac ttcaagaatt
     7081 aaccgaaaat accgtaacag gcggcacaat gatgattact catgctctgg ctgaggaatg
     7141 gaccacttgt gacggtttat tgatgcatga ctggtattta gctttactag cttctgctat
     7201 tgggaaatta gtttaccttg atattccaac agaattatac cgtcagcacg atgctaacgt
     7261 gttgggagct agaacttggt ctaaacgcat gaaaaattgg ttaacccccc atcatttagt
     7321 caacaaatat tggtggttga ttacttcaag ccaaaaacag gctcaattac tattagattt
     7381 accattaaaa ccaaatgacc atgaattggt aacggcctat gtttctttat tggatatgcc
     7441 ttttaccaaa cgcttagcta ccttgaaaag gtacggtttt agaaaaaatc gcatctttca
     7501 cacctttatt tttcgtagtt tagttgttac cttatttggt tacaggagaa agtaagaatg
     7561 aattttttaa caaagaaaaa ccgtatttta ctgagagaaa tggtaaaaac ggattttaaa
     7621 ttacgctatc aagggagcgc aattggctat ctatggtcta tcttaaaacc gcttatgatg
     7681 tttacgatta tgtacttggt atttattcgt tttcttcgcc tgggtggaaa tgtacctcat
     7741 tttccagtag cgcttttatt ggcaaatgtc atctggtctt ttttttcaga agcaactagc
     7801 atgggaatgg tatctattgt atctcgggga gacttgttgc gaaaattgaa cttttctaag
     7861 cacatcattg ttttttcggc agtgttagga gctttaatta attttcttat taatttggtt
     7921 gttgttttaa tttttgcgtt gataaatggt gtgactatat cagggtatgc ttatctctct
     7981 ctttttcttt ttatagaatt agttgtttta gtgcttggaa ttgcattgtt attgtcgaat
     8041 gtctttgttt attatcgtga cttagctcaa gtctgggaag tactattaca agcaggtatg
     8101 tatgccactc caatcattta tccgatcact tttgttttag atagccaccc tttggcggca
     8161 aagttgttga tgctaaatcc agtagcacaa atgattcaag attttcgtta tttattgatt
     8221 gacagggcca acgtaacgat ttggcagatg tcaaccaatt ggttttacat tgttattcca
     8281 tatttagtac catttgttat attatttatt ggcatctttg tctttaagaa aaatgccgat
     8341 agatttgcgg agattattta atgattaata gagaaattgc agtaaaagta cagcatgtta
     8401 gtaaaacgtt taaactgcct acagaagcaa ctaaaagttt tcgcacgact ctggttaatc
     8461 gcctaagagg gattaaagga tttactgaac aacatgtgct aaaagatatt aactttgatg
     8521 tttataaagg cgattttttt ggaattgttg ggcgtaacgg ctcaggaaaa tcaacgctcc
     8581 ttaaaattat ttcccagatc tatgttccag aaaaagggca ggttacagta gatgggaaga
     8641 tggtttcttt tatagagctt ggagtgggat tcaatccaga attaacagga cgagaaaacg
     8701 tctatatgaa cggcgccatg ctgggattta ccaaagacga agttgatgat atgtacaatg
     8761 atattgttga ttttgcagag ttgcatcatt ttatgaatca aaaattaaaa aattattctt
     8821 ctggcatgca ggtacgttta gcattttcag tggctattaa agcccaaggt gacgttctta
     8881 ttttagatga ggtgcttgct gtaggagatg aagcttttca gcgaaaatgt aatgattatt
     8941 ttatggaaag aaaagacagt ggtaagacga ctattttagt gacacatgat atgggagctg
     9001 ttaagaagta ttgtaaccgt gcagttctta ttgaagatgg tcttgttaaa gcatatggag
     9061 aaccatttga tgttgctaat cagtatagtg tagataatac agagacagct gaggatgcaa
     9121 tgaatgctga aaaaatatct gtatctgata ttgccaaaga tttgaaagtg tcattaatct
     9181 caaatccaag aataacacca aatgacacaa tcacatttga agtttcttat gaagttttaa
     9241 aagatgattt tacctacatt gccttttctt taacggatat tgatagaaac atttgggttt
     9301 acaacgacaa ttctttagat tatccaacac aaggtatagg tagaaaatgc atttcctacc
     9361 aatgtcaact atctcaactt aatgatataa aactaaaatt agaagtaact gtccgagata
     9421 agaacggtca gatgctactc ttttcaacag cagaacaatc accccagata gtgattcaaa
     9481 gaaacgatat tgatcaagat gatctgtcag ctctagattc agcaagtggc ttgtatcaac
     9541 gtaatgggaa gtgggttttt cattaatgag taatagctag aagggaattt atgtataaag
     9601 tttcaattat ttgcaccaat tataacaaag ctccttggat atctgatgca cttgacagtt
     9661 ttttaagcca agtgacagat tttgaggttg aaattattgt tattgatgat gcttctacag
     9721 atgactcacg agagatttta aagagttatc aaaaaaagtc ttcaggaaaa attaaattac
     9781 tctttaacga aactaatatt ggaataacaa agacttggat aaaagcatgc ctttatgcaa
     9841 aaggcaaata tattgctaga tgtgatggtg atgattactg gacggatagt tttaaactcc
     9901 aaaaacaagt tgatgtctta gaagcttcca aacggtcacg atggtgtaat actgattttg
     9961 actttgtaaa tagtcaaggc gacttattat atgctaatgc tttttcatct ggtcatacac
    10021 ctttgacaga tacttacgaa aaagttttag ccttaaaagg gatgacgatg gcatcgacat
    10081 ggttagttga tgctgattta atgagacaag taaaccaaaa aatcaatgta gatacaccag
    10141 acgatacttt tgatattcaa ctagagttat ttcaactgac tcaactgacc tatattaaag
    10201 actcaacgac gatttatcgc atgacaaccg attctgattc aagacctact gatacaccaa
    10261 aaatgattta tcgtattcaa aaactacttg atactcagtt aaattactta aaaaagtata
    10321 atcaggtaga tacaaaagaa gtgtctgaac tattattaca acaagatgct aagcaagaaa
    10381 tacgaattca tgagttgagt tgctttattc aagaattaca acaaactatc gtagataaaa
    10441 caaagcagca agaaacacgt gaagtggagt tgcaaaatgt catagaagaa caaaaaaatc
    10501 aactatctga attaagacaa caataccatg ccattattaa ttcacgtcaa tggaaataca
    10561 cgtctaagct cattgctttt attaggagaa aaaaatgaat cgtattttat tatatgttca
    10621 cttcaacaaa tacaataaaa taagtgcaca tgtttactat caattggaac aaatgagatc
    10681 actgttttct aagattgttt ttatctcaaa tagtaaagtg agtcacgaag atttaaaacg
    10741 tctcaaaaac cactgtctta ttgatgagtt tttgcaacgc aaaaataaag gttttgattt
    10801 ttctgcatgg cacgatggct tgattattat gggatttgac aagttggaag agtttgactc
    10861 attgacgatt atgaacgata cttgttttgg tcctatttgg gagatggctc cttattttga
    10921 aaattttgaa gaaaaagaga cagttgactt ttggggaatt actaataata gaggtacaaa
    10981 agcttttaaa gaacatgtcc aaagttattt tatgacgttt aaaaatcaag tcattcaaaa
    11041 caaagttttt caacaatttt ggcaatcgat aatagaatat gaaaacgttc aagaagtgat
    11101 tcagcattat gaaacgcaat taacatccat tctcttaaat gaaggcttta gttatcagac
    11161 ggtctttgat actagaaaag ctgagtcgtc attcatgcca catccggatt tttcatatta
    11221 taatccaaca gcaattttaa aacaccatgt gccttttata aaagtaaaag ctattgatgc
    11281 taaccagcat atcgctcctt acttattgaa tctgatacga gaaacaacca attatcctat
    11341 agatcttatt gtttcacata tgtcgcaaat ctctttgcca gatactaagt accttttatc
    11401 tcaaaaatac ctaaactgcc aacggttagc aaaacaaact tgtcaaaagg tagctgttca
    11461 tttacatgtt ttttacgttg atttgctaga tgaattttta acagctttcg aaaactggaa
    11521 cttccattat gatcttttta ttacaacaga tagtgatatc aaaagaaaag agatcaaaga
    11581 aatacttcaa agaaaaggta aaactgctga tatacgcgtt acgggaaata gaggaagaga
    11641 tatttatcca atgttattac taaaagataa actttctcaa tatgattata ttggtcattt
    11701 ccatacgaaa aaatcaaaag aagctgattt ttgggcagga gaatcttggc gaaaagaatt
    11761 gattgatatg ctcgtgaaac cagcagatag tattcttagc gcttttgaga cagatgatat
    11821 tggtattata atcgcagata ttccaagttt ttttagattt aataagattg tcaatgcttg
    11881 gaatgagcat ttaatcgctc aagaaatgat gtctttgtgg agaaaaatgg atgttaaaaa
    11941 acaaattgat tttcaagcta tggatacatt tgttatgagt tatggaacat ttgtatggtt
    12001 taaatacgat gctctaaaat cactttttga tttagaatta actcaaaatg atattccaag
    12061 tgaaccttta ccacaaaact ctatattaca tgcgattgag cggttacttg tctatatagc
    12121 gtggggggat tcttatgatt ttaggattgt taaaaatccc tatgagttaa caccttttat
    12181 tgacaataaa ctgttaaatt tacgtgaaga tgaaggagct catacctacg ttaactttaa
    12241 tcaaatgggc ggtatcaaag gagcattgaa atatattatc gttggtcccg ctaaagcaat
    12301 gaagtatata ttcttgcgtt taatggagaa acttaaatga ttaaagacac atttttaaaa
    12361 accaattggt taaatattag tcaccatatt atccttcttg ttttcggttt ttatttcagt
    12421 ttttacagtt tggcgaaaga actagtaagc tccacggcac aaccggtaaa ctattatgct
    12481 catttactaa atgtttcttt tgtgggatat attatatcac tgattggatt atcttattat
    12541 ttgagtcgcc aagttagtcg acagttgttt ttgaaaacta gttttattgt gatatcttat
    12601 ctaattgtca gctattgggt acaaataaca cagcacctga atgataaacg gtttgatatc
    12661 tggtcattaa ctaaaaatca attttatcaa tttcaagctc taccttcttt actcattatt
    12721 ttagtgatgg ccactttaat aaaaatattg gtagcatatt ttgcaataga aaaagataga
    12781 tttgggctat taggctatca aggtaatact ttttctgtag ctctgatttt agcagttgtg
    12841 ccaattaatg acatacatct gttaaaacta ataagttctc gattttctga attagtaaca
    12901 gcaggtaata gccaaattgc actgttaaaa ataagtggac tgttgatagt tttacttgtc
    12961 atatttgcaa caatcatata cgtggtttta aatgctctaa aacaccttaa gtcaaataaa
    13021 ccttcatttt cagtagcagc tactactagt ttgtttttag cattagtttt taactatacg
    13081 ttccagtatg gagtaaaagg tgatgaagca ttgctaggat attatgtttt ccctggagct
    13141 actctttttc agatagtagc tattacacta gttgctcttt tagcatacgt gataacgaat
    13201 agatattggc caactacctt ctttttgctt attccgggaa caattatttc tgttgttaat
    13261 gatttaaaag aatcaatgag aagcgagccg ttattagtaa ctgattttgt ttggttacaa
    13321 gaattaggtt tagtgacaag ctttgttaaa aaatcggtga ttgtagaaat ggttgtagga
    13381 cttgctattt gtattgtggt agcttggtat ctacatggcc gagttttagc agggaaatta
    13441 tttatgagcc ctgtcaaacg ggcaagtgct gtattaggtt tatttattgt atcttgtagt
    13501 atgttaatac cattttctta tgaaaaagaa ggtaaaatat tatctggtct tccgattatt
    13561 tcggctttaa ataatgataa tgacataaac tggttaggtt tttcaacaaa tgctaggtac
    13621 aaatctttag catatgtttg gacaagacag gtgaccaaga aaataatgga aaaaccgaca
    13681 aattatagcc aagaaacaat agcgagtatc gctcagaagt accaaaaatt agcagaagat
    13741 attaataaag acagaaaaaa taatattgct gaccaaacgg ttatttatct tttaagtgaa
    13801 agcttgtcag atcctgatag agtatcaaat gttactgtta gccacgatgt tttacctaat
    13861 atcaaggcaa tcaaaaatag cacaactgcg ggactcatgc agtcagactc ctacgggggt
    13921 ggaacggcta acatggagtt tcaaacgtta acaagcttac ctttttataa tttttcttct
    13981 tcagtatctg ttctttattc agaagtcttt cctaaaatgg ccaaacctca tacgattagt
    14041 gagttttacc aaggaaaaaa tcgtattgcg atgcatcctg ctagtgctaa caattttaat
    14101 agaaaaacag tttatagtaa tttaggtttt tccaaattct tagctctatc gggttctaag
    14161 gataagttta agaacattga aaatgtcggt ttattgacta gcgataaaac tgtctataat
    14221 aatattttat ctttaattaa tcctagtgaa agccaatttt tctcagttat tacaatgcaa
    14281 aatcatattc cttggtcatc cgattatcct gaagaaattg ttgctgaagg aaaaaatttc
    14341 acggaagaag aaaatcacaa cctaacaagt tatgctcggt tattatcgtt tactgataag
    14401 gaaacaagag catttttaga aaaattaaca caaattaaca agcctatcac agtggtgttt
    14461 tacggagatc atttacccgg tttatatcct gatagtgctt ttaacaagca tattgaaaat
    14521 aaatacctta ctgattattt tatttggagt aatggtacta acgagaaaaa aaatcatccg
    14581 cttatcaact caagtgattt tactgcagct ttatttgagc atactgattc aaaagtatca
    14641 ccttactatg ctttgttaac agaggtactg aataaagcta gtgtcgataa atcaccagat
    14701 agtcctgaag ttaaagctat tcagaatgat ttaaaaaata tccaatacga tgtgactata
    14761 ggaaaaggtt accttttgaa acacaaaact ttttttaaga tatcacgtta agaaaaatgc
    14821 taggctgtcc gagtataggc aagcatcaag gtttggtaat ttgctcaaat gattgtcaac
    14881 agagtgtcta ggaccaggtt taaaagatta taattcttaa ggtcttattc gtttataaaa
    14941 aatgaatact cttttaaaat cttaattgaa atgaatagga gaagttttcg tgaaaaagtt
    15001 aatcattatt cctgcttaca atgaaagcag taatattgtc aatactatac gtactattga
    15061 atcagatgcc ccggattttg actatatcat tattgatgat tgctcaacgg ataatacgtt
    15121 agcaatatgt caaaaacagg ggttcaatgt tatttctttg cccattaacc tgggaattgg
    15181 cggtgcggtg caaactggct atcgttatgc acaaagatgt ggatatgacg ttgcagttca
    15241 agtagatgga gatggtcagc acaatccatg ctatttggaa aaaatggttg aggtattagt
    15301 tcaatcttca gtaaatatgg taattggatc acgatttatc acaaaagaag ggtttcagtc
    15361 atcatttgct cgacgtatcg gcataaagta ttttacttgg cttattgcgc tactaacagg
    15421 aaaaaaaata acagatgcaa cgtcaggtct aaggttaatt gaccgttcat tgattgaacg
    15481 ttttgctaat cattatccag atgattatcc tgaacctgaa acagttgttg atgtattggt
    15541 tagtcatttt aaagtgaaag aaattcctgt tgtaatgaat gagcgacaag gcggtgtgtc
    15601 atctatctcg ttgacgaaat cggtctatta tatgattaaa gtgactttag ctattttagt
    15661 tgttagattg aaaggaaatc gttaaaatga cggtaacagc cagtttattt ataagtttta
    15721 ttgtattaac ctttgttttc tttttgatta acttaatcaa aaaagataaa ctagcgatta
    15781 agtactcttt attgtggttt attttagctt tattaatttt actatttac
//
LOCUS       DAXWIQ010000038        15628 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000038 DAXWIQ010000000
VERSION     DAXWIQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15628
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.038"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            21..674
                     /gene="grab"
                     /locus_tag="ACGEUI_001332"
     CDS             21..674
                     /gene="grab"
                     /locus_tag="ACGEUI_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G-related alpha2-macroglobulin-binding protein
                     GRAB"
                     /protein_id="HFF8166162.1"
                     /translation="MGKEIKVKCFLRRSAFGLVAVSASVLVGSTVSAVDSPIEQPRII
                     PNGGTLTNLLGNAPEKLALRNEERAIDELKKQAIEDKEATTAIEAASSDALEALADQT
                     DALQSEEAAVVKADNAASDALEALADQTDALQSEEAEVVQSDNAASDAWEKAATPIAL
                     DVKKTKDTKPVVKKEERQNVNTLPTTGEESNPFFTAAALAIMVSTGVLVVSSKCKEN"
     gene            912..1454
                     /locus_tag="ACGEUI_001333"
     CDS             912..1454
                     /locus_tag="ACGEUI_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFF8166163.1"
                     /translation="MDIWTKLAVFAFFETPKVILRPFRYEDHWDFYSMVNDTKNLYYV
                     FPEQKTKAASDYLLVHSFIKFPLGQWAIEDKATHQVIGSIRIEHYDAKTRCADIGYFL
                     NYAFWGQGIMTEVVIKLVYLSFHEFGLKTLRIITHLENKASQKVAKKAGFQLKTCFKG
                     SDRNTHKICIYKMYQLTNDR"
     gene            1463..2746
                     /gene="spxR"
                     /locus_tag="ACGEUI_001334"
     CDS             1463..2746
                     /gene="spxR"
                     /locus_tag="ACGEUI_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS-HotDog domain-containing transcription
                     factor SpxR"
                     /protein_id="HFF8166164.1"
                     /translation="MSKHQDILDYLEKLAIGKKVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETKPRSGTVRIEKKGRVRIDRLTYSEIARISDSEVLAGHAGLGHEFSRFSIGA
                     MTQQNIRRYLVKGGLLIVGDRETIQLLALENHNAILVTGGFPVSKRVIEMANNQRIPV
                     MVTHYDTFTVATMINHALSNIRIKTDLKTVEQVMIPITDYGYLCEDSSVEEFNTLIKK
                     TRQVRFPVLDYKRKVIGVVSMRDVVDQLPTTKLTKVMSKNPITARPNTSLANISQKMI
                     FEDLNMLPVTDEENNLLGMITRRQAMENLPNHQPNNPYTYSEQILSNLEETVDYYQVV
                     VEPTMIDSAGNMSNGVISEFLKEISIRALTKKHQKNIIIEQMMVYFLHAIQIEDELKI
                     YPKIITENRRSSTIDIEIFVDDQVIAKAIITTKIN"
     gene            2762..3622
                     /locus_tag="ACGEUI_001335"
     CDS             2762..3622
                     /locus_tag="ACGEUI_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HFF8166165.1"
                     /translation="MITLKSAREIEAMDRAGDFLAGIHIGLRDIIKPGVDMWEVEAYV
                     RRRCKEDNVLPLQIGVDGHMMDYPYATCCGLNDEVAHAFPRHYILKEGDLLKVDMVLS
                     EPLDKSIVDVAALDFDNVPEMKKWTGSYTGGLADSCWAYAVGTPSDEIKQLMDVTKEA
                     MYRGIEKAVIGNRIGDIGAAVQEYAESFGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLKEGMVLTVEPMINTGTWEIDTDIKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            3624..4589
                     /locus_tag="ACGEUI_001336"
     CDS             3624..4589
                     /locus_tag="ACGEUI_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFF8166166.1"
                     /translation="MAEKKWFDKVLSKWQYEPIQVFMRHLQSAEMDLSAIAVAYYLIL
                     TAFPLIVIAANIFPYLNIDIADLLRLMKQNLPKDIFRPASAIVENIFSKPSGSVLGVA
                     TLTGLWTMSRSLTSLQKAINKAYGASQHRDFFIGHLVGLLTSLIILFLLAFALIFSIF
                     SKAAIQVLDKHYHLSDNITTIFLLLIQPITVLIIFVGLMLLYFLLPNVKIKKIRYILP
                     GTLFTSFVMTFLSNLVGNYVVYNVERMVDIKMFGSVMIFIIMLWFIFLARILILGAIF
                     NATYQEMSLGKLEGRSGDMIAILKKTLGNDSDLSPSQSIEDSHTD"
     gene            4700..5983
                     /gene="aroA"
                     /locus_tag="ACGEUI_001337"
     CDS             4700..5983
                     /gene="aroA"
                     /locus_tag="ACGEUI_001337"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFF8166167.1"
                     /translation="MKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLK
                     GEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQGLNAPCQTLNMGNSGTSMRLIAGLL
                     AGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQPITY
                     TLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVG
                     PQQLTAQEITVPGDISSAAFWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQI
                     VYEDMNKKEQVTSIRVVYSNMKGTIISGGLIPRLIDELPIIALLATQAQGTTCIKDAQ
                     ELRVKETDRIQVVTDILNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAI
                     AALLVKQGQVHLDKEEAIMTSYPTFFKDLERLCHD"
     gene            5976..6467
                     /locus_tag="ACGEUI_001338"
     CDS             5976..6467
                     /locus_tag="ACGEUI_001338"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFF8166168.1"
                     /translation="MTKVLLGFMGVGKTTVSKHLSMHCKDMDAIIEAKIGMSIAAFFE
                     QHGEIAFRTIESQVLKDLLFANDNSIIVTGGGVVVLQENRQLLRKNHQHNILLVASFE
                     TLYQRLKHDKKSQRPLFLKYSKEAFYEFYQQRMVFYEGLSDLVIRVDHRTPEEVANII
                     EGY"
     gene            6675..8126
                     /locus_tag="ACGEUI_001339"
     CDS             6675..8126
                     /locus_tag="ACGEUI_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8166169.1"
                     /translation="MTKYPMGGLSHHEELRYFYLLRNLSYLSENEKKEFAFLKSKLEI
                     GRAYAPSKQHYRKSKRQEPYFEDDYYNDYSPNDLLEDDDVNHDSSFVPYGLPIYPKED
                     RYLNKKTKLTARRPIDAPQPIDEDDAFLTESVARCALPRSQKRKHKKKGCMKWFFNIL
                     GLLLMTVLMGLGLMFAKGVFDISTNKANYKPAVSQAFDGQETQDGTNILILGSDQRVT
                     QGSTDARTDTIMVVNVGNHAKKIKMVSFMRDTLINIPGYSYNDNSYDLKLNSAFNLGE
                     QEDHHGAEYVRRALKHNFDIDIKYYVMVDFETFAEAIDTLFPNGVKIDAKFATVGGVA
                     VDSVEVPDDLRMKNGVVPNQTIEVGEQRMDGRTLLNYARFRKDDEGDFGRTVRQQQVM
                     SAVMSQIKDPTKLFTGSAAIGKIYALTSTNVSFPFVVKNGVSVLGSGKNGVEHVTIPE
                     NGDWVDEYDMYGGQALYIDFDKYQKTLAKLGLR"
     gene            8208..9563
                     /gene="rlmD"
                     /locus_tag="ACGEUI_001340"
     CDS             8208..9563
                     /gene="rlmD"
                     /locus_tag="ACGEUI_001340"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFF8166170.1"
                     /translation="MLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVDNALPEEVIDMR
                     VLKVNKNSGFGKVEAYHYLSSARNADVNLTYLRTGIADLGHLTYEDQLTFKKKQVQDS
                     LYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKHSHDLIPISDYY
                     IQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGEMMLVLVTTRPK
                     VFRVDQVIEKIVEAFPAVVSIIQNINDKNTNAIFGKDFKTLYGKDTITDSMLGNNYAI
                     SAQSFYQVNTVMAEKLYQTAIAFSDLSKDDIVIDAYSGIGTIGLSFAKTVKAVYGVEV
                     IEAAVRDAQQNAALNGITNAYFVADTAEHAMATWAKDGIKPSVILVDPPRKGLTESFI
                     QASVAMGPQKITYVSCNPATMARDIKRYQELGYKLAKVQPVDLFPQTHHVECVVLLIK
                     E"
     gene            9602..10380
                     /locus_tag="ACGEUI_001341"
                     /pseudo
     CDS             9602..10380
                     /locus_tag="ACGEUI_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060407.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
     gene            10465..10866
                     /locus_tag="ACGEUI_001342"
     CDS             10465..10866
                     /locus_tag="ACGEUI_001342"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HFF8166171.1"
                     /translation="MSDLTQEMTLNVISIFDNYQIELAEKGHLILSTEVTETALNYYG
                     NAHGGYLFTLCDQVGGLVARTTGVESVTLQANANYLKAGHKGDKLMVEGRLVHGGRTT
                     QVVDVTIHNQTGALLTKTSLTMFVTGRRHNG"
     gene            complement(10973..12457)
                     /locus_tag="ACGEUI_001343"
     CDS             complement(10973..12457)
                     /locus_tag="ACGEUI_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFF8166172.1"
                     /translation="MKTIAFDSNKYLNLQRDHILERISQFDGKLYMEFGGKMLEDYHA
                     ARVLPGYEPDNKIKLLKELKEQVEIVIAINANNIEHSKARGDLGISYDQEVFRLIDKF
                     NTLDIYVGSVVITQYNNQPAADAFRKQLEKNGIASYLHYPIKGYPTDINHIISSEGMG
                     KNNYIKTSRNLIVVTAPGPGSGKLATCMSQMYHDQINGVKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLETYGKTAVNYNRDIEVFPVLNRTFERILSKSPYA
                     SPTDMGVNMVGFSIVNEEAAIEASKQEIIRRYYQTLVDFKAERVTESAVKKIELLMND
                     IGVTPDDRHVTVAAHQKAEQTGQPALALQLPNGQIVTGKTSELFGPTAAVIINAIKTL
                     AKIDKTTHLIEPEYVKPIQGLKVNHLGSHNPRLHSNEILIALAITAMTSEEANLAMKE
                     LGNLKGSEAHSTVILTEEDKNVLRKLGVNITFDPVYQHHKLYRK"
     gene            12831..14051
                     /locus_tag="ACGEUI_001344"
     CDS             12831..14051
                     /locus_tag="ACGEUI_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8166173.1"
                     /translation="MSTLSLDTTNKRALVAAIAASGTDDLNVMFLAFSMSSIMTDLGL
                     SGTQGGWIATITNLGMLVGGLLFGLLADRHHKFKVFKWTILLFSVATGLIYFTQSLPY
                     LYLMRFIAGIGVGGEYGVAIAIMAGIVPPEKMGRMSSLNGIAGQLGSISSALLAGWLA
                     PSLGWRGLFLFGLLPILLVIWMTLAIDDQKIWDHYGQEEEECSQPIKINELFKTKSLT
                     AQTLALMVMTTVQIAGYFGMMNWLPTIIQTSLNLSVKSSSLWMVATIVGMCLGMLYFG
                     QLLDCFGPRLIYSLFLLASSICVYLFQFANSMASMVIGGAIVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNVILNVGRALGGFSSVAIGSILDASGISMVMIFLASLYVISFGAMW
                     SIGQLKAERYQQLR"
     gene            complement(14171..14536)
                     /locus_tag="ACGEUI_001345"
     CDS             complement(14171..14536)
                     /locus_tag="ACGEUI_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HFF8166174.1"
                     /translation="MTIYSHTYETRPNHSAKAMGSGTLNVLATPALVAFMENASYLFA
                     QEQLDSDFTTVGSEIAIQHLAASAIGDPVTIIITALKEEGRKYDFRLEAFVGEQLIGK
                     ACHTRVRVNSKHFMAKVAE"
     gene            14651..15382
                     /locus_tag="ACGEUI_001346"
     CDS             14651..15382
                     /locus_tag="ACGEUI_001346"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFF8166175.1"
                     /translation="MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEY
                     KDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQQATVMELLDDTARQKAVFPVGRL
                     DKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVAGIMTEADKDYFARGISLKDHQC
                     LPAHLEVLASDLQQQTSLVKITIQEGKFHQVKRMVAACGKEVLDLQRLSMGPLKLDPS
                     LAEGEFRRLTPEELQSLAPYCQSSL"
     gene            complement(15431..>15628)
                     /locus_tag="ACGEUI_001347"
     CDS             complement(15431..>15628)
                     /locus_tag="ACGEUI_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFF8166176.1"
                     /translation="PDNGMMDSFFGILKSEMFYGLETTYQSLDKLEEAITDYIFYYNN
                     KRIKAKLKGLSPVQYRTKSFQ"
BASE COUNT         4850 a         2713 c         3323 g         4742 t
ORIGIN      
        1 aaaataaaaa aggagaaaca atgggaaaag aaataaaagt gaaatgcttt ttgcgtagat
       61 cagcttttgg attagttgcg gtgtcagcat cagtattagt cggttcaaca gtatctgctg
      121 ttgactcacc tatcgaacag cctcgaatta ttccaaatgg cggaacctta actaatcttc
      181 ttggcaatgc tccagaaaaa ctggcattac gtaatgaaga aagagccatt gatgaattaa
      241 aaaaacaagc tattgaggat aaagaagcta cgacagctat agaagcagca agttcagatg
      301 ccttagaagc attagcggat caaacagacg ctttacaatc agaagaagct gcggttgtta
      361 aagcggataa cgctgctagt gacgccttag aagcattggc ggatcaaaca gacgctttac
      421 aatcagaaga agctgaagta gttcaatcag ataacgctgc tagtgacgcc tgggaaaaag
      481 cagcaactcc aatcgcttta gatgttaaga aaactaaaga tacaaaacct gtagttaaaa
      541 aagaagaaag acaaaacgtt aatacccttc ctacaactgg tgaagagtct aacccattct
      601 ttacagctgc tgcgcttgca ataatggtaa gtacaggtgt gttagttgta agttcaaagt
      661 gcaaagaaaa ttagttgcta tttgttctag caaatgaaac aagggaatcg aaagattctc
      721 tttttctgat atttaaaaat gctacagttt ataatagctt gaaagactaa atgttctcaa
      781 actcattaga acaagcttta ataaaactaa cccagttgta tcaaaggata gataaagact
      841 aagactgtta tagaatattt gcaaaagatt tgttataatg gacccaatac aggtattgtg
      901 aaaggtataa tatggatatt tggacaaagc ttgcagtgtt tgcttttttt gagactccga
      961 aagtaatatt acggcctttt cgctatgaag atcattggga tttttacagc atggttaacg
     1021 acactaaaaa cctttattat gtttttccag aacaaaaaac taaggcagca agtgactatc
     1081 ttttagtaca tagttttata aagtttcctt taggtcagtg ggcaatagaa gataaagcaa
     1141 cccaccaagt aataggttct attagaattg agcattatga tgctaaaacg cgttgtgctg
     1201 atattggcta ttttttaaac tatgcctttt ggggacaagg aattatgaca gaagtcgtaa
     1261 taaaacttgt ttatttatct tttcacgaat ttggtctcaa aacgttgcgt attataactc
     1321 atttagaaaa taaggccagc cagaaagtag ctaaaaaagc aggttttcaa ctaaaaacct
     1381 gttttaaagg aagtgatcgt aatactcata aaatctgcat ttataaaatg taccaactaa
     1441 ctaatgatag gtgataatca ttatgagcaa acatcaagat attttagatt atttagaaaa
     1501 acttgctatt ggtaaaaaag tgagtgttag aagcatttca aaccacttaa aagtaagcga
     1561 tggaacagcc taccgtgcta ttaaagaggc agaaaatcga ggtattgtcg aaaccaaacc
     1621 aagaagtgga actgttcgta ttgaaaaaaa aggacgggtt cgtattgacc gtctgaccta
     1681 ttccgaaatt gctcggatca gtgattcgga agtgctagcg ggacatgctg gtttaggaca
     1741 tgaatttagt aggttttcaa ttggtgctat gactcaacaa aatattcgtc gttacttggt
     1801 caaaggaggg ttattaatag ttggggatcg tgaaacgatt caactcttag ctcttgaaaa
     1861 ccataatgcc attttggtga cgggcggatt tccagtatcc aaacgtgtta ttgaaatggc
     1921 aaataaccaa cgaatcccag ttatggtaac tcattatgat acctttactg tggcaacaat
     1981 gattaatcac gcgctatcta atattcgtat taaaacagat ctcaaaacag ttgaacaggt
     2041 aatgatacct attacagatt atggttacct ttgcgaagac agctctgttg aagaattcaa
     2101 tacattaata aaaaaaacac gacaagtcag gtttcctgta ttagattata aaagaaaagt
     2161 gattggtgta gttagtatgc gtgatgtggt ggatcagttg ccaacaacta agcttaccaa
     2221 ggtcatgtct aaaaatccaa taacggctag accaaatact agtcttgcta atattagcca
     2281 gaaaatgatc tttgaagatt taaacatgtt acctgtcact gacgaagaaa acaatttact
     2341 tggtatgatt actcgtagac aagcaatgga aaatttgcca aatcaccaac caaataaccc
     2401 ctatacttat agtgagcaaa tactttctaa ccttgaagaa acggttgatt attatcaagt
     2461 agtagttgag ccaacaatga ttgatagtgc tggtaacatg tcaaacggtg ttatttcaga
     2521 gttcttaaag gaaattagta tcagagccct cacgaaaaag caccaaaaaa atatcattat
     2581 cgaacaaatg atggtatact ttttacatgc tattcaaatt gaggatgaat taaaaatata
     2641 tcctaaaatc attactgaaa atagacgaag cagcaccatt gatattgaaa tttttgtgga
     2701 tgatcaagtg attgctaaag ctattattac aacgaaaata aattagagga gtaacaaaac
     2761 aatgataaca ttaaaatcag cccgtgaaat agaagcgatg gatagagcag gagacttctt
     2821 agcaggtatc catattggct taagggatat tattaagcct ggtgtggata tgtgggaagt
     2881 ggaagcatat gttcgtcgcc gttgtaaaga agacaacgtt cttcctttgc aaataggggt
     2941 tgatggtcac atgatggact atccttatgc gacctgttgt ggtctaaatg acgaagtagc
     3001 acatgccttt ccccgtcatt acattctgaa agaaggggat ttgcttaaag ttgacatggt
     3061 actcagtgag cctttggata aatcaattgt tgatgtagca gctttagatt ttgataacgt
     3121 acctgagatg aagaaatgga caggatctta taccggtggt ttagctgact cttgttgggc
     3181 ttatgctgtt ggaacaccgt ctgacgaaat aaagcaatta atggatgtca ccaaagaagc
     3241 catgtaccgt ggtattgaaa aagcagtaat tggcaatcgt attggtgata ttggcgcagc
     3301 tgttcaagaa tacgctgaaa gcttcggtta tggtgtcgtg cgtgatcttg ttggtcacgg
     3361 tgttggtcct acaatgcatg aagagccaat ggtaccaaac tatggaacag caggtcgtgg
     3421 acttcgtcta aaagaaggca tggtcttgac tgttgagcca atgattaata caggaacttg
     3481 ggaaattgat accgacatca agacaggttg ggctcataaa accctagatg gtggtttatc
     3541 ttgccaatac gaacaccaat ttgtgattac taaagatggt cccgttattt taacaagtca
     3601 aggagaagaa agaacttact aaaatggcag aaaaaaaatg gtttgataag gtactgtcca
     3661 agtggcagta tgaacccatt caagtcttta tgaggcattt acaaagtgca gaaatggatt
     3721 tatctgccat tgcggttgcg tattacctta tcttaacggc ctttccatta attgttattg
     3781 cagccaatat ttttccttac ctcaatattg atattgctga tttgttacgt ttaatgaagc
     3841 aaaatttgcc taaagatatt tttagaccgg cttctgcaat tgtagaaaat attttctcta
     3901 aaccgtctgg aagtgttttg ggagtagcga ctttgacagg attatggaca atgtctcgaa
     3961 gtttgacatc gctacaaaaa gctattaata aagcctatgg cgcttctcag catcgagatt
     4021 tctttatcgg acacttggtt ggtttgttga ccagtttaat tattttattt ttattagcct
     4081 ttgccttaat cttttcaata ttttctaaag ctgctattca agtgctggat aagcattacc
     4141 atttgagtga taatatcaca acgattttct tgctcctcat tcaacctatt acagttttga
     4201 ttatttttgt tggcttgatg ctactatatt ttttattacc caatgttaag attaaaaaaa
     4261 ttcgctacat tttaccagga accctattta catcttttgt gatgacattt ttgagtaatc
     4321 tggttggcaa ttacgttgtg tataatgttg agcggatggt agatattaaa atgtttggtt
     4381 cagttatgat ttttatcatc atgctatggt ttattttctt agcaagaatc ctgattttag
     4441 gagctatatt taatgcgacc tatcaagaaa tgtccttggg gaaattagaa ggtcgtagtg
     4501 gtgatatgat cgctatcctt aaaaaaacac ttgggaatga ttcagatcta tctccttcac
     4561 agtctatcga agatagtcac actgattgat acaaaaaaga taataagtcg tggagtgaga
     4621 aactattttc ttgcttcatt ttttatgagg atgaatagaa aatcattgat cttagcaaat
     4681 agaaaggcag atgaaacgga tgaaattgcg aacaaatgca ggaccattac agggaacgat
     4741 tcaagttcca ggagacaagt ccattagtca ccgggcagtc atattaggag ccgttgctaa
     4801 aggggaaaca cgagtaaaag gactcttaaa aggagaagat gtgctttcca cgattcaagc
     4861 ctttaggaat ttgggggtcc gtatcgaaga aaaagatgac cagcttgtga tagaaggtca
     4921 aggttttcaa ggattgaacg ctccttgtca gacacttaat atgggaaatt ctggaacctc
     4981 tatgcgcctc attgctggct tattggcagg tcaacctttt agtgtcaaaa tgattggcga
     5041 tgaaagctta tccaaacgtc ctatggatcg gattgtttat ccgttaaagc aaatgggggt
     5101 tgagatatct ggagaaacag ataggcaatt tcctccacta caattgcaag gtaatcgtaa
     5161 tttacaacca atcacatata ccttgcctat ctcttctgct caggttaagt cagctatttt
     5221 attagcagct ttgcaagcaa aaggaacaac acaggttgtt gaaaaagaga ttactcgaaa
     5281 ccatacagaa gaaatgattc aacaatttgg tggacgattg atagttgatg gtaaacgcat
     5341 tactttggtg ggaccccagc agctcactgc tcaagagata actgtccccg gagatatttc
     5401 tagtgcagct ttttggcttg tcgccggctt aattattcct ggttcggagc tccttttaaa
     5461 gaatgttgga gtcaatccga cacgaacagg gattcttgaa gtggttgaaa aaatgggagc
     5521 acagattgtc tatgaagata tgaataagaa agagcaagta actagtattc gagtggtata
     5581 ttctaacatg aaaggaacta tcatatcagg aggtcttatc cctcgattga ttgatgaact
     5641 gcccatcatt gcacttcttg caacccaagc acaaggaaca acctgtatta aagatgctca
     5701 agaattacga gtcaaagaaa cagatcgtat tcaagtagta actgatattc ttaacagtat
     5761 gggagctaat atcaaagcga cagcagatgg catgattatc aaaggcccta cggtactata
     5821 tggggctaac acaagtacct atggagatca tcgtattggt atgatgacag caattgcagc
     5881 cttgcttgtt aaacaaggac aggtccattt ggataaagaa gaagccatca tgacgtctta
     5941 tccaacgttt ttcaaagatt tagaaaggct gtgtcatgac taaagtgtta ttgggattta
     6001 tgggagtggg gaaaacaacg gtttcgaaac acctaagtat gcactgtaaa gatatggatg
     6061 ctattattga agctaaaatc ggtatgtcta ttgcagcttt ttttgaacag catggagaaa
     6121 tagcatttcg aacaattgaa agtcaagtgt taaaagatct attgtttgca aatgataata
     6181 gtatcatcgt aacaggtggc ggtgtagtgg ttttacaaga aaatcgacaa ttactgagaa
     6241 aaaatcacca gcataatatt ttattggtag cttcttttga aactttgtac cagcgtttaa
     6301 aacatgataa aaagtctcaa agacctctat ttcttaagta ctctaaggaa gcattttatg
     6361 aattttatca acaacgtatg gtattttatg agggactttc agatttagta atcagagttg
     6421 accatcgtac gcctgaagaa gttgccaaca tcattgaggg ttattagtag gtcattgtcg
     6481 taatctgata tttatggttt taaacaggta tctgtctaaa gcacttctta tggcaagttg
     6541 gctttattaa atattaggaa ataatcttga ttattctata atgattaaaa ttttgagagg
     6601 acaatctggc taatataggt tataatggta gtattcgtta aaagtgcaat tgtaatacta
     6661 agagatgata agatatgaca aaatacccca tgggtggcct tagtcaccat gaagaattac
     6721 gttattttta tctcctaagg aatttgagtt accttagtga gaatgaaaaa aaagagtttg
     6781 cttttttaaa atctaaattg gaaattggtc gagcttatgc tccttctaag cagcattata
     6841 ggaagtcaaa acggcaagaa ccttactttg aagatgacta ctataacgac tactcaccaa
     6901 atgatttact agaagatgat gacgtcaatc atgactcttc ttttgttcca tatggtttac
     6961 cgatatatcc taaagaagac agatatttaa acaaaaaaac aaaacttaca gctcgtagac
     7021 ctatagatgc tccgcagcct attgatgaag acgatgcttt tttaacagag tctgttgctc
     7081 gttgtgctct tcctagatct caaaaaagga agcataagaa aaaaggatgt atgaaatggt
     7141 tttttaacat tcttggctta ttgctaatga ctgtgcttat ggggttaggg ttgatgtttg
     7201 caaaaggggt ttttgatatt tctactaata aagctaatta caaaccagct gtttcccaag
     7261 cttttgatgg tcaagaaact caagatggta cgaacattct gattttgggt tctgaccaac
     7321 gagttactca aggttctaca gatgctcgca cagataccat tatggttgtc aatgtcggca
     7381 accatgctaa aaaaataaaa atggtctctt ttatgcgtga cactttgatt aacattccag
     7441 gatacagtta taatgataat tcgtatgatc ttaaattaaa tagtgcattt aatcttggag
     7501 agcaagaaga tcaccacgga gcagagtacg ttcgacgggc cttaaaacat aattttgata
     7561 tcgacattaa gtattatgtg atggtagatt ttgaaacatt tgcagaagct attgacacct
     7621 tgtttccaaa tggtgttaag attgatgcca aatttgcgac agtaggtggt gtagctgttg
     7681 actccgtaga agttccagat gacttgcgaa tgaaaaatgg ggttgttccc aaccaaacca
     7741 tcgaagttgg agaacaacgc atggatggta gaaccttgct caactatgct cgtttccgta
     7801 aagatgacga aggagatttc ggacgaaccg tccggcagca acaggttatg tcagctgtta
     7861 tgtcccaaat caaagaccca actaaactct ttacagggtc tgcggctatt ggtaaaatct
     7921 atgcgctgac ttcgaccaat gtttctttcc catttgttgt taaaaatggt gtttctgttt
     7981 taggaagtgg gaaaaatgga gttgagcacg tgaccatccc agaaaatggg gattgggttg
     8041 acgaatacga tatgtatgga ggacaagccc tttacataga ttttgataag tatcagaaaa
     8101 cgttagccaa attaggtctg cgctaagata tggtataatg gagagtaact aaagttgctc
     8161 ttcttttttg agcaatggtt agccctagaa aaggaaaaag aataagaatg ttgaaaaaaa
     8221 atgatattat ccaagttgct atttcagatt taagccatga gggagcaggt gtcgctaaac
     8281 acgatggttt tgttttcttt gttgataatg ccttgccaga agaagtgatt gacatgcgtg
     8341 tcttaaaggt caataaaaac agcggttttg gcaaagtaga agcctatcat tacctgtcgt
     8401 cagctagaaa tgcagatgtc aatttaactt atttgcgaac aggcattgca gaccttggac
     8461 atctaactta tgaagaccaa ctgactttca aaaagaaaca agttcaggac agcctctata
     8521 aaattgctgg aatcagtgac gtgacagtcg aaagcacgat tgggatgaca gaacctttag
     8581 cttaccgaaa taaggctcaa gttcctgtta gacgtgttaa tggtcagcta gaaacaggct
     8641 ttttccgcaa gcattctcat gacttgattc ctatttcgga ttattatatt caggacaaag
     8701 agattgatcg tctcattaat tttacccgtg atttattacg ccgctttgat attaaaccgt
     8761 atgacgaaac agaacaaaca ggtctcttga gaaatattgt tgttcgccga ggtcattact
     8821 ctggagaaat gatgctggtc ttagtgacta cacgcccaaa agttttccgt gttgaccaag
     8881 tcattgaaaa aatcgttgaa gcttttccag cagtggtgtc tattattcaa aatattaatg
     8941 ataagaacac caatgcaatc tttggtaaag atttcaaaac gctttacggc aaggatacca
     9001 ttactgatag catgcttgga aacaactatg ccatttctgc tcagtctttc taccaagtga
     9061 atacggttat ggcagaaaaa ctctatcaaa cagctatagc cttttcagac ctttctaaag
     9121 atgatattgt gattgacgcc tactcaggta tcggaaccat tggcctatca tttgctaaaa
     9181 ccgttaaagc tgtttatggt gttgaagtga ttgaagcagc tgttagagat gctcaacaga
     9241 atgccgctct taatggcatc accaatgcct actttgtcgc agataccgca gaacatgcca
     9301 tggcaacatg ggcgaaagat ggcatcaaac caagtgttat tctggtagac ccgccacgaa
     9361 aaggcttaac agaaagtttc atccaagcca gcgtggcaat gggaccacaa aaaattacct
     9421 acgtttcctg caacccagcc accatggcgc gtgacataaa gcgttatcaa gagttgggtt
     9481 acaaactggc caaagtacag cccgtagatt tgtttccgca aacgcatcac gtcgagtgcg
     9541 tagtgttgct aatcaaggag taaggtagct gcctccacat gacaaaaata aggagaaacg
     9601 aatgcctatt accaaacacg agattcccct tttagaatgt gatagtaatc catcagccgt
     9661 cattatctag ctatttagct tccagctaag tgtgtctatg ctttcttaga agacgagatt
     9721 gaagcttttg ctttagacca tcaaggtaaa aaagtagcag aattcatttc agccacaaag
     9781 gtttaccctg tttatcagat atcttataaa ggccaagaca tttgcctagc tcaagctccc
     9841 gttggctcag ctcctgcggc gcaattcatg gactggctga ttggttatgg ggttaaagaa
     9901 atgatcagta caggaacctg tggcgttttg gttcctatcg cagaaaaccg ttttttagtg
     9961 ccagttaagg ctttgcgaga tgaaggaaca agttatcatt acgtggcacc atctcgttat
    10021 attgatattg atcctaagat gcttagactg attgagaaaa cgcttttagc acaggggtta
    10081 gcttatcaag aggtcatcac ctggtcaaca gatggttttt accgagaaac caaagaaaaa
    10141 gtggcttatc gccaggaaga agggtgttcg gttgttgaga tggaatgttc tgccttagca
    10201 gcagtagctc aattaagagg catcctttgg gggcaactct tatttacagc agatactcta
    10261 gcagatgtag aggtttacga tcaacgcaac tggggcgctg attcctttag ttttgcgctc
    10321 catttatgct tggaagtgct caatacgcta gaaaaggatg gaaaggctac tgatttttga
    10381 aacatgagct ctgccttctg ttcctatttg tagtataatg aggcttgtat tgcttttaga
    10441 ctaagcttag aaaagagaaa aacaatgtca gatttaacac aagagatgac cttaaatgtt
    10501 attagtattt ttgataatta ccaaattgaa cttgctgaaa aaggacacct tattttaagt
    10561 actgaagtga ctgagacggc tttgaattat tatgggaatg cccatggtgg ttatcttttc
    10621 actctatgtg atcaagtagg tggtttggtt gctagaacta ctggcgtaga atctgtcacc
    10681 ttacaggcca atgctaacta tttaaaagca gggcacaagg gagacaagct gatggttgaa
    10741 ggtcgattgg ttcatggagg tcgaaccacg caggtggttg atgtgacgat tcataaccaa
    10801 acaggagctc ttctaacaaa aacaagcttg accatgtttg tgactggccg acgtcataac
    10861 gggtgattaa tgaatcatag aaggctaaaa aagtcccaac tctctgagaa ttgggacttt
    10921 tttcctttat tgagagatac cataaatcac ccgttagacg ggtggttatg gtttatttac
    10981 ggtaaagttt gtgatgttgg taaacagggt caaaggtaat atttacccca agttttcgga
    11041 gtacattttt atcttcttcg gttaagataa cggtagaatg ggcttcgcta cctttcaaat
    11101 tgccaagttc tttcatggct agattagctt cttcactagt catagcagta atggccaacg
    11161 caatcagaat ttcattggaa tgaagccgcg gattgtgact gcctaagtgg ttcactttga
    11221 ggccttgaat aggtttcacg tattcgggtt caatcaaatg agtggtttta tcaatctttg
    11281 ccaaggtttt aatcgcattg atgataacag ctgcagttgg tccaaagagt tcagatgttt
    11341 tacctgtcac aatctgccca tttggtagtt ggagagcaag ggcaggttgg cctgtttgct
    11401 ctgctttttg atgagcagct acagtaacgt gacgatcgtc aggggtgaca ccgatatcat
    11461 tcatcaagag ctcaattttt ttgacagcag attctgtgac tcgctctgcc ttgaaatcca
    11521 cgagggtttg gtaataacga cggatgattt cttgtttaga agcttcgata gcagcttctt
    11581 cattgacgat agaaaaacca accatgttaa cacccatatc ggttggtgag gcataaggag
    11641 acttgctcaa aatacgctcg aaggtccgat ttaagacagg aaagacctcg atatcacggt
    11701 tgtaattaac ggccgttttg ccataagttt ccaaatggaa ggggtcaatc atattcacat
    11761 catctaggtc tgccgtggct gcctcataag ccagattgac agggtgatga agaggaagat
    11821 tccaaactgg gaaggtttca aatttagcat agccagactt aaccccattg atttggtcgt
    11881 gatacatttg ggacatgcaa gtggctaatt ttccagaccc agggcctggt gctgtaacaa
    11941 cgataaggtt acgactcgtt ttgatgtaat tatttttccc catcccctca gatgagataa
    12001 tatgattaat atctgtcggg tagcccttga taggataatg caggtaagaa gcaatgccat
    12061 ttttttcgag ttgcttgcga aaggcatctg cagcgggttg gttattgtat tgagtaataa
    12121 cgacagaacc tacgtaaata tctaacgtgt taaatttgtc aattaagcga aagacttctt
    12181 ggtcataaga aatgcctaaa tcaccacgcg ctttagaatg ttcaatatta ttagcgttaa
    12241 tggcaattac aatttcgact tgttctttga gctctttgag taatttaatc ttattgtcag
    12301 gttcatagcc aggaagcaca cgggcagcat ggtaatcttc taacatttta ccaccaaatt
    12361 ccatgtaaag cttgccatca aattggctga tacgttctaa aatatgatct ctttgcaagt
    12421 ttaaatactt gttagaatca aaagcaatag tcttcatttt ttcacctctg taaagcatta
    12481 taacaaagaa attccaagaa agaaaaggtt ttttgcaggg atacctcatt ttgtgacttt
    12541 ttaaaaaaac ttaggcttgg ttcttagaag aaaaatgtat ccgctatgtt caaaagaact
    12601 tgacaattaa aatgagcttt actatactag taacaattaa atactacttt aaacatgttc
    12661 cgtgaagcat gaaagtctta acagtaaaga gagttttttt gtcatctctt attatttagt
    12721 gaactttgat ggacttgcgg acttgtttcc aagtcttttt ttgacctttg gacaaagggt
    12781 attcaattaa ttaaggtagt cagtgagcta ctattaagaa ggagacaatt atgtcaacat
    12841 tatcattgga caccacaaat aagcgagctt tagtggcagc tattgcagct tcaggaacgg
    12901 atgacttgaa tgtcatgttt cttgcctttt ccatgtcttc cattatgaca gacttgggtc
    12961 ttagtggcac tcagggtggc tggattgcga ccattaccaa tttaggaatg ttggtgggag
    13021 gcctcttatt cgggttgctg gctgataggc accacaagtt taaggtcttt aaatggacga
    13081 ttttactgtt ttcagtagca acaggattaa tttattttac ccagtctttg ccctacctgt
    13141 acctgatgcg atttattgct ggcataggag ttggaggtga gtacggtgta gccattgcca
    13201 taatggcagg gattgtacca ccagaaaaaa tgggtcggat gtcttcttta aatggtattg
    13261 ctggccagct tggctctatt agttcggctc ttttagcagg gtggctggct ccaagtctag
    13321 gctggcgtgg cctttttctc tttggattac ttccgattct cttggtgatt tggatgactt
    13381 tagccataga tgaccaaaaa atttgggatc attatgggca agaggaggaa gaatgcagtc
    13441 aaccaattaa aatcaatgaa ctattcaaaa caaaatcctt aacagctcaa acccttgctt
    13501 taatggtcat gacgactgtg cagattgcgg gctattttgg aatgatgaat tggttgccaa
    13561 ccattattca aacaagctta aacctttcag taaaaagttc gtctttgtgg atggtggcta
    13621 ctattgtcgg catgtgtttg gggatgctgt attttggtca gcttttagat tgctttggtc
    13681 cacgtctgat ttattccctc tttttattag catcatctat ttgtgtttac ctttttcaat
    13741 ttgccaattc catggcgagt atggttattg gaggtgctat tgtcggtttt tttgtcaatg
    13801 ggatgtttgc tggctacggg gctatgatta ccagacttta tccccatcac attcgatcca
    13861 cggctaataa tgttattttg aatgttggcc gtgctctagg aggattttcg tccgttgcta
    13921 ttggtagtat tttagatgcc tcaggtattt caatggtcat gattttccta gcttctctgt
    13981 atgtgattag ttttggagcc atgtggtcta ttggtcagtt aaaagcagaa cgctaccaac
    14041 aattgcgtta gcaaagagat gggtgttagt atttgagtta ctttcactca caaagctatt
    14101 ttattgattg agataaccaa aaaggtaagc acttagggat tgcttacctt tttggttctt
    14161 ttcttttgtt ttactccgct actttggcca taaaatgttt actgttcacc ctaactctag
    14221 tatgacaggc ttttccaatg agttgttctc ccacaaaagc ttccaagcgg aaatcgtatt
    14281 ttcggccttc ttctttaagg gctgtaatga taattgtaac aggatctcca atggctgaag
    14341 ctgccaaatg ttggatagct atctcgctgc caaccgttgt aaagtcactg tcgagctgtt
    14401 cttgggcaaa taagtaagaa gcattttcca taaaagcaac aagagcagga gtagctagga
    14461 cattcagcgt tcctgagccc atggctttag ccgaatgatt gggacgggtt tcataagtgt
    14521 gagaataaat agtcataagt taatctcctc gtttctataa gcctattata atatattaag
    14581 cgcttacagt catgaaacta ctgttttttt atgttatact taataatgaa ttttctttga
    14641 aagggttatg atgcgcttag ataaattttt agtggcaact ggtgttggaa ctaggagcca
    14701 agtcaaactt ctcttaaaaa agaaggctat tttcgtcaat caaaaggttg aaacctctgc
    14761 caaagcacac attgatgaat acaaggattt ggtgacgtat caaggcacgc ccttggtcta
    14821 tgaatccttt gtttattacc tgttaaacaa gccgtcaggg tatgtgtcag caacccagga
    14881 cagacagcag gctactgtta tggagttgtt agatgacacg gctaggcaaa aagcagtttt
    14941 tcctgtgggt aggttagata aggatacccg tggacttttg ctcttgacca ataatggaca
    15001 actagcccat gaccttttat cccctaaaaa acatgttacg aaagagtact tggctaaagt
    15061 agcaggcatc atgactgaag ctgataaaga ttattttgct agaggtattt ccttaaagga
    15121 tcaccagtgc ctacctgctc atttagaagt gcttgctagt gatttacagc agcaaacaag
    15181 tttggttaaa attaccatac aagaaggaaa attccatcag gtgaaacgca tggtagcagc
    15241 ctgtggaaag gaagtgcttg acttacagcg tctcagcatg ggaccattaa aactagaccc
    15301 tagccttgca gaaggggagt ttaggcgcct gacgcctgag gaattgcaga gcctagcacc
    15361 ttattgtcag agctcactat aaaaagaaac ctccccggtc agtactgacc ccaaaaagtt
    15421 ggacaaataa ttattgaaag gatttagttc tgtattggac aggactaagt ccttttagtt
    15481 ttgctttaat tcgtttgttg ttgtagtaaa aaatgtaatc tgtaatagct tcttcaagct
    15541 tgtcaagtga ttgataagtt gtctcgagtc cataaaacat ttcagatttg agaatgccaa
    15601 agaaggaatc catcatgccg ttatccgg
//
LOCUS       DAXWIQ010000039        15409 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000039 DAXWIQ010000000
VERSION     DAXWIQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15409
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.039"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            102..350
                     /locus_tag="ACGEUI_001348"
     CDS             102..350
                     /locus_tag="ACGEUI_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8166177.1"
                     /translation="MGLIWTLIVGALIGVIAGALTKKGGSMGWIANIAAGLVGSSVGQ
                     ALLGSWGPSLAGMSLIPSVIGAVIVVMITSFVLSKTNN"
     gene            411..998
                     /gene="amaP"
                     /locus_tag="ACGEUI_001349"
     CDS             411..998
                     /gene="amaP"
                     /locus_tag="ACGEUI_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFF8166178.1"
                     /translation="MSKLLKISYCLVGLVLLSVFGWVVGITGGYIYLPYSYRWLSWGM
                     DSFPNLLDSALSYYYFWTALVLFVITFLALLVIILYPRIYTEVQLRHKNKKGTLLLKK
                     SAIESYVATAIQTAGLMPNPTVTAKLYKRKFNIIVKGRLASRVAVADQISGVKEGIEK
                     GLTEFFGINYPVNFKVYVKDIADSDRKHITRNRVE"
     gene            1009..1197
                     /locus_tag="ACGEUI_001350"
     CDS             1009..1197
                     /locus_tag="ACGEUI_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFF8166179.1"
                     /translation="MEFYEKFKYPIIGGLVGLIIAILLMAFGLFKTLLAIIFIILGIY
                     GGLYAKKTGIIDQFLNRK"
     gene            1210..1749
                     /locus_tag="ACGEUI_001351"
     CDS             1210..1749
                     /locus_tag="ACGEUI_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8166180.1"
                     /translation="MTETYIKNTSKDLTSAIRGQLTYDDKVIEKIVGLALENVDGLLG
                     VNGGFFANLKDKLVNTESVRDGVNVEVGKKQVAVDLDIVAEYQKHVPTIYDSIKSIVE
                     EEVKRMTDLDVIEVNVKVVDIKTKEQFEAEKVSLQDKVSDMARSTSEFTSHQVENVKA
                     SVDNGVEKLQDQKAEPRVK"
     gene            1790..1990
                     /locus_tag="ACGEUI_001352"
     CDS             1790..1990
                     /locus_tag="ACGEUI_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFF8166181.1"
                     /translation="MSDEKYNAKLDQAGGKLKEGFGKISGDKSLETEGKVDKVTGKVK
                     EVIADAKDTVKGLAKGLDNKDK"
     gene            2001..2489
                     /locus_tag="ACGEUI_001353"
     CDS             2001..2489
                     /locus_tag="ACGEUI_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8166182.1"
                     /translation="MEKTQIKSTLTYDDKVIEKIVGHALENVGGLLAVTGGFFSNIKN
                     NLVNSESVTDGVSVEVGSKEVAVDLAIIVEYGKDIPAIAESIKAIVSQNVDSMTHLKV
                     VEVNVNVVDIRTKEEHEAASVTVQDRVTSAASSTSQFVSEQTEKLKDTISDTVNSDEA
                     AK"
     gene            2652..3293
                     /locus_tag="ACGEUI_001354"
     CDS             2652..3293
                     /locus_tag="ACGEUI_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8166183.1"
                     /translation="MISPKKEITSSKYQKIAISVAQRIANGEYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLKILTLKHGSGAIVLSKERAIEFINQYESTHSIAVLKEKIR
                     ETINDQGKAMEKMAVLVNDFLMQSQSVSKQYPLAPYEIICNQDSEHFGKSIGVLNIWH
                     QTGATIVAIEHAGQFIVSPGPYSVIEKGDHIYFVGDESVISRMKTFFNLRKGL"
     gene            complement(3436..3978)
                     /locus_tag="ACGEUI_001355"
     CDS             complement(3436..3978)
                     /locus_tag="ACGEUI_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8166184.1"
                     /translation="MTKRHTETKAYVKTALTTLLTEQSFETLTVSDLTKKAGINRGTF
                     YLHYTDKFDMMNHFKNDTLDDLYRLLNQAEIYTDTRQVLNQTLSYLIEHREFITALAT
                     ISYLKFPQLIKDFCYQFLTTITGFQDIVTNQYHIPYPYALEVYLASIESIISYWIANG
                     YQETTEEVADIILKAVTLDN"
     gene            4096..4797
                     /locus_tag="ACGEUI_001356"
     CDS             4096..4797
                     /locus_tag="ACGEUI_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8166185.1"
                     /translation="MAFIELKQVSKSYQIGETTVFANHEVSFEINKGELVVILGASGA
                     GKSTVLNILGGMDTVDAGQVIIDGKDIAHYTSKALTQYRRNAIGFVFQFYNLVPNLTA
                     KENVELAVEIVADALDPVTILKEVGLSHRLDHFPAQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDYQTGKQILTLLQDMAQTKGTTVVIVTHNAAIAPIADRVIFMHDAQVTK
                     TVINKEPASIETIDY"
     gene            4812..7448
                     /locus_tag="ACGEUI_001357"
     CDS             4812..7448
                     /locus_tag="ACGEUI_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFF8166186.1"
                     /translation="MIKKTLWKDILRAIKNSKGRFISLFFLMALGSFALVGLKVTGPD
                     MERTASRYLERHQVMDLTVLASHQFSQADKQELDTLKGAHLEYGHLLDVSLTSNQKSL
                     RLYSVPKKVSKPVLVKGSWPKRETDLVLSSSLAKNYQIGDELAVTSPMEGLLTTTHFQ
                     VVGFANSSEVWSKSNLGSSSTGDGSLYAYAFVNPNVFKSAFNLLRIRFSHLRLTNAFS
                     KDYQKKVTQNQAHLDNLLKDNGQKRYDDLQNQYDLALKNGRAALAKETVKLAASEENL
                     TFLEGSALQEAKHQIEQGKQALAKEEKQLEQVQATKDKLEKPSYLTYNRSTLPGGEGY
                     HTYATSTTSISNVGNIFPVVLYLVAALVAFTTMTRYVDEERTSSGLLKAIGYSNKDIS
                     LKFLIYGLLASFLGTTLGIIGGTYLLSTLISEILTGALTIGKTHLYSYWFYNGIAYLL
                     AMLSAVLPAYLIVKKELFLNAAQLLLPKPPSKGAKIWLEHLTFVWKALSFTHKVTIRN
                     IFRYKQRMLMTIVGVAGSVALLFAGLGIQSSLAKVVEHQFGDLTTYDILAVGSAKATA
                     TEQTDLASYLKQEPITGYQKVSYASLTLPVKGLPDKQSISILSSSATSLSPYFNLLDS
                     QEQKKVPIPTSGVLISEKLASYYKVKPGDQLILTDRKGQSYKVTIKQVIDMTVGHYLI
                     MSDTYFKNHFKGLEAAPAYLIKVKDKDSKHIKETASDLLTLKAIRAVSQNVNHIKSVQ
                     LVVTSLNQVMTLLVFLSILLAIVILYNLTTINIAERIRELSTIKVLGFYDQEVTLYIY
                     RETISLSLVGILLGIYLGKGLHTYIMTMISTGDIQFGVKVDAYVYLVPILVILSLLAV
                     LGIWVNRHLKKVDMLEALKSID"
     gene            7540..8136
                     /locus_tag="ACGEUI_001358"
     CDS             7540..8136
                     /locus_tag="ACGEUI_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFF8166187.1"
                     /translation="MENSIEAYLADSREKVTLGTVVDCFKGKAVSSKVVPGDVGLINL
                     SDMGTLGIQYHQLRTFQMDRRQLLRYLLEDGDVLIASKGTLKKVCVFHKQNRDVVASS
                     NITVLRPQKLLRGYYIKFFLDSPIGQALLDVADHGKDVINLSTKELLDIPIPVIPLVK
                     QDYLINHYLRGLTDYHRKLNRAEQEWEYIQNEIQKGLG"
     gene            8147..9103
                     /locus_tag="ACGEUI_001359"
     CDS             8147..9103
                     /locus_tag="ACGEUI_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFF8166188.1"
                     /translation="MGSVERTLKMTLATIVAILIAYQLHLDYAMSAGIIALLSVLDTR
                     KSSLVVARNRLLSFFLAFGIAMMCFSLFGFTTVGFMCYLLIIIPLLYHFQIEAGLVPI
                     TVLVTHLIAKKSIALPILSNEFMLFFVGTSVALLFNAYMGPQDQQIRYYHQKVESDLK
                     GILYRFESFLLEGKGQNEGLLIKNLDKILDEALKLVYRERHNQLFQQTNYQVHYFEMR
                     RQQNRLLGQMAINVNTLMRQSKESILLSHLFHETACQLSEQNPALTLIDDIEQLLETF
                     RHGDLPQTREEFERRAVLFQLLQDLERFILLKVEFYQDYQND"
     gene            9200..10540
                     /locus_tag="ACGEUI_001360"
     CDS             9200..10540
                     /locus_tag="ACGEUI_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HFF8166189.1"
                     /translation="MNEIKCPHCHTLFTINESEYSQLLEQVRGQAFDEELKKRLINEI
                     ALLEEKAKHQLHEVVAKKETAITSLTNQLEQIEKEQAYLRQEELAKKDQLIASLEAKL
                     DKLASQNALELANQLAEKDKEVVSLTNQLDKLALEKDATFQSKLATIEKERDGIKNQL
                     ALQAKESELSLASVRSDYEAQLKAANEQVEFYKNFKAQQSTKAIGESLELYAETEFNK
                     VRSYAFPNASFVKDNQLSSRGSKGDYIYREVDANGVEILSIMFEMKNEADTTKTKHKN
                     SDFFKELDKDRREKDCEYAVLVSMLEADNDYYNTGIVDVSHEYQKMYVVRPQLFIQLI
                     GILRNAALNSLHYKQELALVKEQNIDITHFEEDLDQFKNAFAKNYQSASNNFKKAIDE
                     IDKSIKRMEEVKRFLTTSENQLRLANNKLEDVSVKKLTRQNPTMREKFEALKDQ"
     gene            10656..11540
                     /gene="truB"
                     /locus_tag="ACGEUI_001361"
     CDS             10656..11540
                     /gene="truB"
                     /locus_tag="ACGEUI_001361"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFF8166190.1"
                     /translation="MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTEAGKVYEGQVTLGYSTTTEDASGEVVARSSLPAVLTEELVD
                     QTMTTFLGKITQTPPMYSAVKVNGRKLYEYARAGESVERPRREVTISLFERTSPLNFT
                     EDGLCRFSFKVACSKGTYVRTLAVDLGRALGVESHMSFLQRSASAGLTLETAYTLGEI
                     ADMVSKQEMSFLLPIEYGVADLPKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKV
                     IAILEKRDQEYKPKKVLI"
     gene            11569..12501
                     /locus_tag="ACGEUI_001362"
     CDS             11569..12501
                     /locus_tag="ACGEUI_001362"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFF8166191.1"
                     /translation="MEIEYIKDYRDINQEDDTVLILGYFDGLHRGHKALFDKAREVAN
                     KEGLKVVVFTFTESPKLAFSRFSPELLLHITYPKKRYEKFADYGVNKLYLVDFTSKFS
                     KVSSDHFITHYIKNLKAKHIVVGFDYKFGHNRTDSDYLTRNFEGQVYTIEEIKEDHRK
                     ISATWIRKLIQEGNVVKANHLLGYDLSTRGRVVHGDARGRTIGFPTANLAPIDNTYLP
                     ADGVYVTNVIVANKIYRSMTSLGKNVTFGGKELRLEVNIFDFDEEIYGEIIEIVWLDK
                     IRDMEKFEGIEDLTDRLEYDKRTALNWKKDSKLS"
     gene            12544..12948
                     /locus_tag="ACGEUI_001363"
     CDS             12544..12948
                     /locus_tag="ACGEUI_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8166192.1"
                     /translation="MVTLFLSPSCTSCRKARAWLVKHEVDFQEHNIITSPLSRDELMS
                     ILSFTENGTEDIISTRSKVFQKLDIDVEELSISDLIDLIAKNPSLLRRPIIMDQKRMQ
                     IGFNEDEIRAFLSRDYRKQELRQATIKAEIEG"
     gene            12950..13228
                     /locus_tag="ACGEUI_001364"
     CDS             12950..13228
                     /locus_tag="ACGEUI_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFF8166193.1"
                     /translation="MSGNYYYPLDLSWSTEEISSVLHFLNKVELAYEKKVDAKQLLDS
                     YKTYKTIVKSKAQEKQIDRDFQKVSGYSTYQVVKKAKAIEKGFFSLGN"
     gene            13218..14006
                     /locus_tag="ACGEUI_001365"
     CDS             13218..14006
                     /locus_tag="ACGEUI_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFF8166194.1"
                     /translation="METKYAFARQIIKEAGLFIKSKMSEQLDIQVKTQFDDLVTNVDQ
                     ETQQLLMDRIHQTYPCDAILAEENDVRHPINQGNVWVIDPIDGTVNFIVQGSQFAVMI
                     AYYEQGIGQFGLIYDVMADQLLAGGGDFEVTLNGDKLPAYQEKPLERSLIGCNAGMFA
                     RNDRNLAHLIAKTLGVRVYGGAGICMVKVMKQELLAYFSFIQPWDYAAAKVLGDKLGY
                     VLLTIDGYEPDFQTRQKIMFVPKCQLTRIASFLTKDSAITLEDQ"
     gene            14012..15319
                     /locus_tag="ACGEUI_001366"
     CDS             14012..15319
                     /locus_tag="ACGEUI_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFF8166195.1"
                     /translation="MSLPKEFINTYQAILGKELEDFLASFNQEPVNAFRINPLKNQLK
                     TFEHPIPNTLWGYYGKLSGKSPEHVSGLVYSQEPAAQMVAQVAAPQKGSRVLDLAAAP
                     GGKSTHLLAYLDNTGLLVSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFSH
                     YFDTIVFDGPCSGEGMFRKDPDAIQYWHHGYPAECAKLQKSILEDALAMLKPGGELIY
                     STCTWAPEENEDVVQWLLETYTFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQF
                     VAKLKDKRQEGQSTKLKAPKSNLIKDQLRLWKMFEKDHLNLTLSGTLQTFGDYLYLLP
                     DGLPALDGLKIARNGLELGVFKKKRFEPSYALGLALKPDEVRSSIELREEDFRPYVSG
                     NVITLPQSYPTGWYQLLINGNGLGFAKVVDKTIKNNFPKGLRF"
BASE COUNT         4967 a         2544 c         3198 g         4700 t
ORIGIN      
        1 ctttttgaaa gtcgactatt aaaagactat tgcttgaaga atgacagctg ttattcttcc
       61 ttggacaaaa ggtacaggag tatctaaaaa ggagaattat catgggactt atttggactt
      121 taatcgtcgg tgcgcttatt ggtgtgattg ctggagcact tactaaaaaa ggtggttcaa
      181 tgggctggat tgcaaacatc gctgctggtc tagttggttc ttccgttggt caagctttac
      241 ttggttcatg gggcccttct ttagcaggaa tgtctttgat cccgtcagtt attggtgccg
      301 ttatcgttgt tatgatcact tcttttgtac taagcaaaac taacaactag tttcctcttt
      361 atttagtaag tacttacaaa tagagaatgt taagtagaaa agaggtctgt atgtcaaaat
      421 tgttaaagat tagttattgt cttgtcggac ttgttctatt gtctgttttt ggatgggtag
      481 ttggaataac cggaggctat atatatttac cttatagtta tcgttggcta agttggggta
      541 tggatagttt tcccaatctc ttagattcag ctttatccta ttattacttc tggacagccc
      601 ttgtattatt tgttataaca tttcttgcct tattagttat tattttatac ccaagaattt
      661 atactgaggt ccagttacgc cataaaaata aaaaaggaac acttttatta aaaaaatcgg
      721 ctattgaaag ttatgttgcg accgctattc aaactgcagg tttgatgcca aacccaacag
      781 tcacagctaa attatataag cggaagttta acattatcgt taaaggacgt ttagcttctc
      841 gtgtagcagt ggcagatcaa attagtggcg tgaaagaagg gatcgaaaaa ggtttaaccg
      901 agttcttcgg aattaattat cctgttaatt tcaaagttta tgttaaagat attgctgatt
      961 ctgatcgtaa acatattacc agaaatcgtg tagaatagga gatctatcat ggaattttat
     1021 gaaaaattca agtaccctat tattggtggc ttggtggggc taattattgc catattgctc
     1081 atggcttttg gtttattcaa aacgctacta gcaattattt tcattatttt aggaatatat
     1141 ggtggcctgt atgccaaaaa aacaggtatt attgatcaat ttcttaatcg taaataaagg
     1201 agacttatta tgactgaaac ttatattaaa aacacatcta aagacttaac ttcagcaatt
     1261 cgtggccaat taacatatga tgataaagta attgaaaaaa ttgttggttt agcacttgaa
     1321 aatgttgatg ggttactggg tgtaaatggt ggtttttttg ctaacctaaa agataaatta
     1381 gtaaacaccg aatctgtacg tgatggcgtg aacgtggaag ttggaaaaaa acaagttgcc
     1441 gttgatcttg atattgtcgc tgaatatcaa aagcatgtgc cgactattta tgactctatt
     1501 aaatcgatcg ttgaagaaga agtgaagaga atgaccgatc tggatgtgat tgaagttaat
     1561 gttaaagtag tcgacattaa aacaaaagaa caatttgaag ctgaaaaagt aagtttacaa
     1621 gacaaagttt cagacatggc acgttcaaca tctgaattta cgagtcatca agttgagaat
     1681 gttaaagctt cagtggataa cggtgttgaa aaacttcaag atcaaaaagc tgagccacgt
     1741 gtaaaataat catatgtcgt caattaggta agctaaagga gggtttattg tgtcagatga
     1801 aaaatataat gcgaaattag atcaagctgg tggcaagtta aaagaaggat ttggtaagat
     1861 ttctggagat aaatctttag aaactgaagg taaagtagat aaagttactg gaaaagttaa
     1921 agaggttatt gcagacgcca aagatactgt aaaaggatta gctaaaggct tagataataa
     1981 agataaataa aggggacatg atggaaaaaa ctcaaattaa aagcactctt acttatgacg
     2041 ataaggtcat cgaaaaaatt gtcggtcatg ctttggaaaa cgttggcgga ttactagctg
     2101 taacaggtgg atttttctca aatatcaaaa ataatttggt gaattctgag tctgtaactg
     2161 atggtgtttc agttgaagtt ggtagcaagg aagtagcagt tgatttagca atcattgtag
     2221 aatacggtaa agatattcct gctattgcag aatctattaa agctattgtt tctcaaaacg
     2281 ttgacagtat gacacatttg aaagttgttg aagtcaatgt gaacgttgtt gatattcgta
     2341 ctaaggaaga acatgaagct gctagcgtta ctgttcaaga tcgtgtcact agtgcagctt
     2401 caagtacttc acaatttgta tcagaacaaa cagaaaaatt aaaagatact atctctgata
     2461 ctgtaaatag tgatgaggca gctaaataag caatcgtcta ggaagtatta ttgactggtg
     2521 gtgataagtt tgagctggta tttaccagct ctttttgtca cggttaaaaa gatacggact
     2581 attgtcatct ctctaaaaat atgatttaat tagatataaa tttttcaaat tattagaaag
     2641 gagtaaatta agtgataagt cctaaaaaag agataacaag ttcaaaatat caaaaaattg
     2701 ctatttcagt agctcagcgt atcgcaaatg gcgaatatga agtgggcgaa aagttaaagt
     2761 caagaactac tatcgcctcg acatttaatg tatcaccaga aacggctcgt aaaggcctga
     2821 atattttagc tgacttgaaa attttgactt taaaacatgg tagtggagct attgtgctgt
     2881 caaaagaaag agctattgag tttataaatc aatacgaatc aacccattcc atcgctgttt
     2941 taaaagaaaa aatacgtgag actattaatg atcagggaaa agcaatggaa aaaatggctg
     3001 ttttggttaa tgatttttta atgcaaagtc aatctgttag taagcaatac ccattagcac
     3061 cttatgagat tatttgtaat caagattcgg aacattttgg caaatctatt ggtgtgttaa
     3121 atatctggca ccagacagga gcaactatcg tcgctattga acatgctggt cagtttatcg
     3181 tttcaccagg tccttattct gttattgaaa aaggcgatca tatttatttc gttggtgatg
     3241 agtcagtcat ttcacgaatg aagacctttt ttaatttacg aaaaggatta taagtttgag
     3301 ggcccacctc aaactttttt tgttgctgtt gagcattatt aaaagaggca gatcatcatg
     3361 gtttgtcttt ccaaaatatc tgtttagaca gttgtttggg attgggagca tgatttcaga
     3421 ctcttttgat gttttttaat tgtctagagt aactgctttg agaataatat cggcaacctc
     3481 ttcagtggtt tcttgatagc cattagcaat ccaatagctg ataatgctct caatactggc
     3541 aaggtaaact tctaaagcat agggataagg aatatggtat tgattggtca caatgtcttg
     3601 gaaaccagta atagtagtca aaaattggta acagaaatcc ttgatgagtt gcggaaattt
     3661 gaggtaagaa atagtagcga gtgcggttat aaattctcta tgttctataa ggtaggacaa
     3721 ggtttgattg aggacttgtc gagtgtctgt gtagatttca gcctgattta acaaacggta
     3781 caaatcatcc agagtgtcat ttttgaagtg attcatcatg tcaaatttat cagtgtagtg
     3841 gaggtagaag gttcctctat tgataccagc ctttttagta aggtcagaga cagtaagagt
     3901 ttcaaaagat tgttcagtta gtaaggtggt taaggcggtc tttacataag ctttggtttc
     3961 agtatgtctt ttggtcattc tcaggtgctc cttaaatcaa cagttgcgtc agatttgttc
     4021 ttgttaaata cttagggcca ttatataata aggctataat taaatcaaca ctatgttcaa
     4081 ataatggagg ggcagatggc ttttattgaa ttaaaacagg tttcaaaatc ttatcagata
     4141 ggagagacga ctgtttttgc caaccacgaa gtgtcttttg agattaataa aggagaacta
     4201 gtggttattt tgggagcatc cggagctggt aaatcgacag tcctcaatat tttggggggc
     4261 atggacacag tagatgcagg tcaagtaatt attgatggca aagacattgc tcattacacg
     4321 tctaaggcct taactcagta tcgtcggaat gctattggtt tcgtttttca attttataat
     4381 ttggttccta atttgacagc taaagaaaat gttgaattag cagttgaaat tgtagcagat
     4441 gctttagatc ctgtgaccat tttaaaggaa gtaggactca gtcatcgtct ggatcatttt
     4501 cctgctcagc tctcaggtgg tgaacagcaa cgggtttcga tagcacgtgc cttagctaaa
     4561 aaccctaaat tgcttctttg tgatgaacct acaggtgccc ttgactacca aacaggaaag
     4621 caaattctaa ccctcttaca ggatatggca caaactaagg ggaccacggt agttattgta
     4681 acccacaatg ctgctattgc tccgatagct gatcgtgtca tttttatgca tgatgcacag
     4741 gttaccaaga cagtcattaa taaggaacct gcaagcattg agacaattga ttattaagag
     4801 gaaggtgtcg tatgataaag aaaaccttat ggaaggatat tttgagagct atcaagaata
     4861 gtaaagggcg attcatttcc ttatttttcc taatggcctt aggctccttt gctttagttg
     4921 gacttaaagt caccggtcca gatatggaac gaacagccag ccgttacctc gaaagacacc
     4981 aggtgatgga cctaacggta ctggcttctc atcaattttc ccaagccgat aaacaagagt
     5041 tagatacgtt aaaaggggct catttagaat atggtcattt acttgatgtc agtctaacta
     5101 gcaaccagaa atctcttagg ctctatagcg tgccaaagaa agtgtctaag ccagtcttgg
     5161 ttaaagggag ttggccaaag cgagagacag atttggtttt atcctcctca cttgctaaaa
     5221 actatcagat tggagatgaa ctagcagtca cctcacctat ggaaggtttg ctgacgacaa
     5281 cccattttca agtggttggt tttgcgaatt cttcagaagt ttggtccaag tccaatttag
     5341 gaagttcctc aaccggagat ggaagtctct acgcttatgc ttttgttaat ccaaacgttt
     5401 ttaaaagtgc ctttaacctg ctgcgcatcc gttttagtca tcttcgtctg acaaacgctt
     5461 tttccaaaga ctatcaaaaa aaggtaaccc aaaaccaagc tcatttggat aatctactta
     5521 aagataatgg ccagaagcgt tatgatgacc tccaaaatca gtatgacctt gccttaaaaa
     5581 atggcagagc agcacttgca aaggaaacag tgaaactagc tgcgagtgag gagaacttaa
     5641 cttttttaga aggctctgct ttacaagaag ctaagcatca gattgaacaa ggcaaacaag
     5701 cattagccaa ggaggaaaag cagttagagc aggtgcaggc tacaaaagat aagctagaaa
     5761 aacccagtta cctgacttat aatcgctcga ccctaccagg aggagaagga tatcatactt
     5821 atgcaacttc aacgacctcc atttcaaatg ttggaaatat ttttcctgtt gttctttatc
     5881 tcgtagctgc tctagtagcc tttaccacca tgacacgtta tgttgatgaa gaaagaacaa
     5941 gctctggtct attaaaagcc attggttatt ctaacaagga tatcagttta aagtttctta
     6001 tctatgggct tttagccagt tttttaggga caactttagg tattattggg ggaacttacc
     6061 tcttatctac cttgatttca gagatcttaa caggagcttt gactattgga aagactcacc
     6121 tttatagtta ttggttttat aatggcatag cttacttgct ggctatgtta tctgctgttt
     6181 taccagccta cttaattgtc aaaaaggaat tattcctcaa tgcagctcag ttattgctgc
     6241 ccaaacctcc tagtaagggg gcaaaaatct ggttggaaca ccttactttt gtctggaaag
     6301 ccctgtcctt tactcacaag gtgaccatac gtaatatttt tcgctataaa caaagaatgc
     6361 tgatgaccat tgtaggcgtt gcaggctcag tagccctttt atttgcaggc ttagggattc
     6421 agtcgtcatt agccaaagta gttgagcatc aatttggtga tttaacgact tatgatattt
     6481 tggctgtcgg ttcggccaaa gcgacagcga cagagcaaac tgacttagct agctatctta
     6541 aacaagaacc tattacaggg taccaaaagg tatcttatgc cagcttaacc cttcctgtaa
     6601 agggattacc tgataagcaa agtatttcca ttttatcaag ttcagctact tctcttagtc
     6661 cctattttaa tctgctggat agtcaggagc aaaagaaggt tcccattcca acctctggtg
     6721 ttttgatttc tgagaaatta gcctcctatt acaaggtaaa accaggtgat cagttgatat
     6781 tgactgatcg gaaaggacag tcttataaag tgacgattaa acaggttatt gacatgacag
     6841 ttggccatta cctgataatg tctgatacct attttaagaa tcattttaaa ggattggagg
     6901 ctgctcctgc ctatctgatt aaggtaaaag acaaagatag caagcacata aaggagacag
     6961 ccagtgactt gttaacctta aaagcgatta gagcagtttc acaaaacgtc aatcatatta
     7021 aatctgttca gctagtagtc acctctctta atcaggtcat gaccctcctt gtcttcttgt
     7081 ctattttatt agcaatcgtt atcctttata acttaacgac tattaatatt gctgagcgta
     7141 ttcgagaatt atccactatt aaagttctgg gattttacga tcaggaggtc accttatata
     7201 tttatcgaga aactatttcg ctatccctag taggcattct tttaggtatc tatttaggaa
     7261 aaggcctgca tacttatatc atgacaatga tttcaactgg ggatattcaa tttggtgtaa
     7321 aggttgatgc ttatgtttat ctagtgccaa ttctagtaat ccttagcttg ttagcggtat
     7381 taggtatctg ggttaatcgc catttaaaaa aggttgatat gttagaagct ttgaaatcca
     7441 tagattgatt ggtgaaaaga cagatataga cagcacttga aaagttaatc atattatgat
     7501 agtatctaaa tactataatt taatagaaag atactatgta tggaaaatag cattgaggcc
     7561 tatctagcag atagcagaga aaaggtaaca ttaggcacag ttgttgattg ttttaaagga
     7621 aaagcagtct caagtaaggt tgtgccaggt gatgtgggat tgattaattt atctgacatg
     7681 ggcaccttag ggattcaata tcaccaatta agaactttcc agatggatcg taggcagctc
     7741 ttgcgttacc ttttggaaga cggagatgtc ttaatcgcct ctaagggaac cctaaaaaaa
     7801 gtctgtgttt ttcataagca aaatcgagat gtggtggcat catcaaatat cactgtctta
     7861 cgccctcaaa aactgttaag gggctactat atcaagttct ttttggactc acctattggt
     7921 caagcgctct tggatgtagc ggaccatggc aaagatgtga tcaatctctc aacgaaagaa
     7981 ttgttggata ttccaattcc cgttattcct ttagtgaaac aggactattt gattaatcac
     8041 tacttgagag gattaacaga ttatcatcgt aaactcaatc gagcagaaca ggagtgggaa
     8101 tatattcaga atgaaattca aaaaggatta ggttaggagt cttgtcatgg gttcagttga
     8161 acgtacatta aaaatgacct tggctaccat tgttgccatt ttaattgctt accaacttca
     8221 tctggattat gcaatgtcag cggggatcat tgccctgtta agtgttttag acacccgtaa
     8281 gtccagtttg gtggtcgcta ggaatcgctt gctatctttt ttcctggctt ttgggattgc
     8341 gatgatgtgc tttagcttgt ttggttttac gacagtaggt tttatgtgtt atttgttaat
     8401 tattattcct ttgctctatc actttcagat agaggcagga ctggttccta taacagtact
     8461 tgtgacacat ttgattgcta aaaaaagtat tgcattacct attttatcga atgagtttat
     8521 gctctttttt gtagggacta gtgtcgcttt actatttaat gcttatatgg gtccacaaga
     8581 tcagcaaatt cggtactatc accaaaaagt tgaatcggat ctgaaaggta tcttataccg
     8641 ttttgaaagc tttctactgg aaggaaaagg tcaaaatgaa gggctcttga tcaaaaacct
     8701 tgataaaata ttagatgaag ctctaaaact agtttatcga gaaaggcata atcaactatt
     8761 tcagcagacc aattatcaag ttcattattt tgagatgcga cgacaacaaa atagactatt
     8821 gggacaaatg gctatcaacg taaacacgtt aatgagacaa agtaaggaaa gtattctttt
     8881 gtcacatctt tttcacgaaa ctgcttgtca gctaagcgaa caaaatccgg ctttaacctt
     8941 gattgatgac atcgaacaat tgcttgaaac ctttcgtcat ggtgatcttc ctcaaactcg
     9001 ggaggaattt gagcgacgtg cggttttatt tcagctctta caagacttag agcgctttat
     9061 cttattaaag gtagagtttt atcaggatta tcaaaatgac taaatctcta ttgcacactt
     9121 cttatctatg acagcttggg acttaacttc ctgagctttt ttgctataat aaaatgaaga
     9181 atctcagaat ggagacaaga tgaacgaaat aaaatgccct cattgtcata ccctatttac
     9241 tatcaatgag tcagagtata gtcaattatt ggaacaagtc agagggcaag cctttgatga
     9301 agaattgaaa aaacggctaa tcaatgagat agccttgctg gaagaaaaag cgaagcatca
     9361 attgcatgag gttgtcgcaa aaaaagaaac agctattacc agcttgacaa accaattaga
     9421 gcaaattgaa aaagaacaag cctacctcag acaagaagaa ctggctaaaa aagaccagct
     9481 tattgcttct ttagaagcga aacttgataa attagcctcg caaaatgctt tggaactggc
     9541 caatcaatta gctgagaagg acaaagaagt ggttagtttg accaatcagc tggacaaatt
     9601 agcacttgaa aaagatgcca cgttccagtc aaaacttgct acaatcgaaa aagaacgcga
     9661 tggaatcaaa aaccaattgg cgcttcaagc taaagaaagt gaattgtcgt tggcttcggt
     9721 gcgcagcgac tacgaagcac agttaaaagc ggctaacgag caagttgaat tttataaaaa
     9781 tttcaaagcg cagcaatcta ccaaagcaat cggagagagt ctagaactct atgctgaaac
     9841 agagtttaac aaagtaagaa gctatgcttt ccccaatgct agctttgtca aagataacca
     9901 attgtctagt cgtgggtcaa aaggggacta tatttaccga gaggttgatg ccaatggtgt
     9961 agaaatcctc tctatcatgt ttgagatgaa gaacgaggca gacaccacca aaacaaaaca
    10021 taaaaatagt gattttttca aggagttaga taaagaccgt cgcgaaaaag attgtgagta
    10081 tgctgttttg gtaagcatgt tagaagctga taatgattac tacaatacag ggattgttga
    10141 tgtcagtcat gagtatcaaa agatgtatgt ggttcgtcct caactcttta ttcagttgat
    10201 agggattctg cgaaatgcgg ccctgaatag tttacactat aagcaagagt tggccttggt
    10261 taaggaacaa aatattgaca tcactcactt cgaagaggat ttggaccaat ttaagaatgc
    10321 ctttgctaag aactatcagt ctgccagcaa caactttaaa aaggctattg acgagattga
    10381 taaatcgatt aaacgcatgg aggaagtgaa acgcttttta accactagtg aaaatcagtt
    10441 acgcttagct aataataaat tagaggatgt ttcagttaaa aaactgaccc gccaaaaccc
    10501 aaccatgcga gagaaatttg aagccctcaa ggaccagtaa gcctgaaagt gagtctttcc
    10561 ctgctaaaat tggttaagcg tttattttgg caaaactgat gataaagcgg tagaatagaa
    10621 tcagtaacaa ttactaatta gagagaaaaa tagacatgat aaatggaatc attaatttaa
    10681 aaaaagaagc cggcatgacc tctcatgacg cagtttttaa attacggaag ttattacaag
    10741 aaaaaaagat tggacatggg gggaccttag atcctgatgt ggtcggtgtt ctaccaattg
    10801 cagttggtaa ggctactcgt gtgatcgaat acatgacaga agctggtaaa gtttatgaag
    10861 gacaagtgac tctcggttac tcaacaacga ctgaggatgc tagtggagag gtagttgcga
    10921 ggtcctcttt gccagctgtg ctaacagaag aactcgttga tcaaacgatg acgactttcc
    10981 ttgggaaaat cacacagacc ccacctatgt attcagccgt aaaggttaat ggccgtaaat
    11041 tatacgagta tgcgcgtgct ggtgaaagtg tggaacgtcc tagaagagaa gtgactattt
    11101 ctctgtttga acgcaccagc cctttgaatt ttactgaaga cggtctttgc cgattctctt
    11161 ttaaagttgc ttgtagcaaa gggacttatg tgcggacctt agcagttgat ttaggtcgag
    11221 ctctaggggt agagagtcat atgtccttct tgcagcgatc agcttcagca ggattaaccc
    11281 tcgagactgc ctatactttg ggagaaattg ctgatatggt ttctaaacaa gagatgtctt
    11341 ttttgttacc gatcgagtat ggcgttgctg atcttcctaa aatggttatt gatgatactg
    11401 aattgactga aatttcattt ggtcgccgtc ttagtctccc tagtcaagag ccactgttag
    11461 ctgcttttca tggcgaaaaa gtaattgcta ttttagaaaa aagagatcaa gaatacaaac
    11521 ccaaaaaagt tcttatttaa atagtttgag taagtagaat ggataattat ggaaattgaa
    11581 tatatcaaag attatcgtga tattaaccaa gaagatgata cagtcttgat attgggatat
    11641 tttgatggat tacaccgtgg tcataaagcc ttatttgaca aagcaagaga ggttgcaaat
    11701 aaagaagggt taaaagtagt cgtttttacg tttacagagt ctccgaaatt ggctttttcg
    11761 cgtttttctc ctgagttatt gcttcatatt acttatccaa agaagcgtta tgaaaaattt
    11821 gccgattacg gtgtaaataa attatatttg gttgatttta cttctaaatt ctctaaggtc
    11881 tcatctgatc attttataac tcattatatt aaaaacctga aagcaaagca tattgttgtc
    11941 ggttttgact ataagtttgg gcataatcgt acagatagtg attatttaac cagaaatttt
    12001 gaaggacaag tttataccat tgaggaaata aaagaagatc atcgaaaaat ttctgctaca
    12061 tggataagga agcttattca agaaggtaat gtagttaaag caaaccactt gttaggctat
    12121 gacttatcga caaggggaag agtcgttcac ggggatgcgc gtggccgtac tattggtttc
    12181 ccaacagcca atctagctcc aattgacaac acatacttac cagctgatgg agtctatgtt
    12241 acaaatgtta ttgttgctaa taaaatctat cgctctatga cgagtttagg taaaaatgtg
    12301 acatttgggg ggaaagagtt gcgtctagaa gttaacattt ttgactttga cgaagagatc
    12361 tatggagaaa ttattgagat tgtttggtta gataagattc gagacatgga aaaattcgag
    12421 ggaatcgaag atttgactga tagattggaa tatgataaac gaacagcatt aaattggaaa
    12481 aaagatagca aattatctta aatttttgta taataaggta agtaaatttg atagaggaaa
    12541 aatatggtta ccttattttt atcacctagc tgtaccagtt gtcgtaaagc aagggcatgg
    12601 ttagtaaaac acgaagttga ctttcaagag cataatatta ttacaagtcc attaagccgt
    12661 gatgaattaa tgtcaatcct ctcttttaca gagaatggaa cggaggatat tatttcaaca
    12721 cgttcaaagg tttttcaaaa acttgatatt gatgtagaag agctttcaat ttcagattta
    12781 attgatttaa ttgctaaaaa tccaagtctt cttcgtcgcc ctattatcat ggatcaaaaa
    12841 cgcatgcaaa ttggttttaa tgaagatgaa attagagcgt ttttatcgag agattatcgc
    12901 aagcaagaac tacgtcaagc aacgataaaa gcagaaattg aggggtgata tgtcaggtaa
    12961 ttattattat ccacttgact taagttggag cactgaagaa atatcctcag tgcttcattt
    13021 tttgaataaa gtggaattag catatgaaaa aaaagtagat gcaaaacaac tcctagatag
    13081 ttataagact tataaaacca ttgtcaaaag taaagctcag gaaaagcaaa tcgatcgtga
    13141 ttttcaaaaa gttagtggtt attcgaccta tcaagttgtt aaaaaagcga aggcaattga
    13201 gaaaggattc ttttcccttg gaaactaaat acgcatttgc aaggcaaatc attaaagaag
    13261 cagggctttt tatcaagtca aaaatgtcag agcagctaga tattcaggtg aaaacacaat
    13321 ttgatgatct ggttaccaat gttgatcaag aaactcaaca gttactgatg gatcgtattc
    13381 atcagaccta tccttgtgat gccattttag ctgaagaaaa tgacgttcgt caccccatta
    13441 atcaaggaaa tgtttgggtc atcgatccca ttgatggaac ggttaacttt attgtgcagg
    13501 gatcacagtt tgcagtgatg attgcctatt atgaacaagg aattggtcaa tttgggctta
    13561 tttatgatgt gatggcagat caattactag ctggtggtgg tgattttgaa gttactttaa
    13621 acggtgataa gcttccagct taccaagaaa aacctttaga gcgtagctta attggctgca
    13681 atgcaggcat gtttgccaga aatgatcgta accttgctca tctaattgca aagactttag
    13741 gtgtaagggt ttatggtggt gcaggtatct gcatggttaa ggtcatgaag caggaattac
    13801 tggcctattt ttcatttatc caaccatggg attacgcagc agcaaaagta ctaggcgata
    13861 aactgggtta tgttttacta actattgacg gatatgagcc tgattttcaa acacgtcaaa
    13921 aaatcatgtt tgttcctaaa tgccaactca ctaggatagc gtcttttcta acaaaggact
    13981 ctgccatcac acttgaagat caatagaaat gatgtcatta ccaaaagaat ttattaacac
    14041 ttatcaagca atactaggca aagaattaga agatttcttg gcaagtttta accaagagcc
    14101 ggtcaatgct tttcggatta accccttaaa gaatcaacta aaaacctttg aacaccccat
    14161 tccaaatacc ttatggggat attatggtaa actatctggc aagtctccag agcatgtgtc
    14221 ggggttggtt tattcccaag aaccagcggc tcaaatggtg gcacaagtag cggctcctca
    14281 aaaggggagt cgtgttttag acttggcagc tgcaccagga ggaaaatcaa ctcatttatt
    14341 agcttattta gacaatacag gtcttttagt cagcaatgaa atttctaaga agcgcagcaa
    14401 ggttttagtt gaaaatatcg aacgttttgg cgctagaaat gttgtggtga ctaatgaatc
    14461 cgcagatcgt ttagccaagg tattttctca ttattttgat actatcgtct ttgatggacc
    14521 atgttctggt gaaggaatgt ttcgcaagga ccctgatgct attcaatatt ggcatcacgg
    14581 gtatcccgca gaatgtgcca agttgcaaaa atctatttta gaagacgcct tagccatgtt
    14641 aaaaccagga ggagaattga tatactccac ctgtacttgg gctcctgaag aaaatgaaga
    14701 tgttgtgcag tggctcttag agacttatac ctttttagaa ttggtcgatg ttccaaaact
    14761 aaatggaatg gttagtggta tcggacttcc agagactgct agaatgtacc ctcatcgtta
    14821 tcaaggggaa gggcaatttg tggccaagct aaaagataag cgtcaagaag gacaaagcac
    14881 taaactaaaa gcacctaaat ccaacttgat caaagaccag ttacgtcttt ggaaaatgtt
    14941 tgaaaaagac catctaaacc ttaccctatc aggaacacta caaacctttg gtgattacct
    15001 atacctcttg ccagatggtc ttcctgcttt agatggcctt aaaattgctc ggaacggttt
    15061 agaattaggc gtcttcaaga agaaacgctt tgaaccctct tacgccttgg gattagcttt
    15121 aaagcctgat gaggtccgat catctattga gcttagagag gaagacttta gaccttatgt
    15181 gtcaggtaac gtcattactt tgccccaatc ttatccaaca ggctggtacc aattactcat
    15241 taatggcaat ggtctaggat ttgccaaggt cgttgacaag accatcaaaa ataatttccc
    15301 taaagggtta agattttagc gtaaaaaata agattaaaaa gttaaatatt taaagaaaaa
    15361 atgctttcaa agcataggta gatatgttac tataaagaat atgtggcac
//
LOCUS       DAXWIQ010000040        14626 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000040 DAXWIQ010000000
VERSION     DAXWIQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14626
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.040"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            35..739
                     /gene="purC"
                     /locus_tag="ACGEUI_001367"
     CDS             35..739
                     /gene="purC"
                     /locus_tag="ACGEUI_001367"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFF8166196.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNKAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSK
                     RFGVEEGHVLETPIVEFYYKNDDLDDPFINDEHVKFLGIVNDEEIAYLKGETRRINEL
                     LKGWFAQIGLNLIDFKLEFGFDQEGTIILADEFSPDNCRLWDKNGNHMDKDVFRRDLG
                     NLTDVYQVVLEKLIAL"
     gene            863..4588
                     /locus_tag="ACGEUI_001368"
     CDS             863..4588
                     /locus_tag="ACGEUI_001368"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFF8166197.1"
                     /translation="MNKRIFVEKKADFGIKSASLVKELTHNLQLTSLKALRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLFGSDSQVKVNTAQLYLVNKDITEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPNLDFFETYQADDFATYKAEQGLAMEVDDLLFIQNYFKSIGCVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQTTYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRPNDVDAFIA
                     ACNKENIDAVVVATVTEKPNLVMTWNGEIIVDLERRFLDTNGVRVVVDAKVVDKDLTV
                     PEARTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFGQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEDLTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKDNFSQFEAIQAQHKIT
                     AASAAKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADAVKIG
                     QTQADFTVTVNGNDLAGASLLAAFEGKLEEVYPTEFEQTDVLEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEVAIAESVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNEKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLAGVEVGDIHAIPVS
                     HGEGKLVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQILFASAVKYFTGK"
     gene            4749..6203
                     /gene="purF"
                     /locus_tag="ACGEUI_001369"
     CDS             4749..6203
                     /gene="purF"
                     /locus_tag="ACGEUI_001369"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFF8166198.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNSSFMGKVKE
                     ALNTVKGGFAYLLMTENKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDRGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHSVDEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            6231..7253
                     /gene="purM"
                     /locus_tag="ACGEUI_001370"
     CDS             6231..7253
                     /gene="purM"
                     /locus_tag="ACGEUI_001370"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFF8166199.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLVSGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYIATGKNNPVKLEEVVSGVAEGCVQAGAALIGGETAEMPGMYGQDDYDLAGFAV
                     GVAEKSQIIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVKAALPLIKEELVKGIGHITGGGFIENIPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     ASVVIK"
     gene            7421..7975
                     /gene="purN"
                     /locus_tag="ACGEUI_001371"
     CDS             7421..7975
                     /gene="purN"
                     /locus_tag="ACGEUI_001371"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFF8166200.1"
                     /translation="MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKVAYEQAIVDLLDKHEIDLVCLAGYMKIVGETLLLAYERRIINIHP
                     AYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGVERKVI"
     gene            8159..9706
                     /gene="purH"
                     /locus_tag="ACGEUI_001372"
     CDS             8159..9706
                     /gene="purH"
                     /locus_tag="ACGEUI_001372"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFF8166201.1"
                     /translation="MTKRALISVSDKSGIVDFAKELKNLGWDIISTGGTKVTLDDAGV
                     ETIAIDDVTRFPEMMDGRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDITYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     GQTTFETRQRLAAKVFRHTAAYDALIAEYFTTQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHM
                     NPCGIGQADDIETAWDYTYKADPVSIFGGIIVLNREVDAATAKKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEATALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQDSIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(9764..10888)
                     /locus_tag="ACGEUI_001373"
     CDS             complement(9764..10888)
                     /locus_tag="ACGEUI_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8166202.1"
                     /translation="MKKFHRFLVSGVILLGFNGLVPTMPSTLISQQENLVHAAVLGDN
                     YPSKWKKGNGIDSWNMYIRQCTSFAAFRLSSANGFQLPKGYGNACTWGHIAKNQGYPV
                     NKTPSIGAIAWFDKNAYQSNAAYGHVAWVADIRGDTVTIEEYNYNAGQGPERYHKRQI
                     PKSQVSGYIHFKDLSSQTSHSYPRQLKHISQASFDPSGTYHFTTRLPVKGQTSIDSPD
                     LAYYEAGQSVYYDKVVTAGGYTWLSYLSFSGNRRYIPIKEPAQSVVQNDNTKPSIKVG
                     DTVTFPGVFRVDQLVNNLIVNKELAGGDPTPLNWIDPTPLDETDNQGKVLGDQILRVG
                     EYFIVTGSYKVLKIDQPSNGIYVQIGSRGTWVNADKANKL"
     gene            11141..12406
                     /gene="purD"
                     /locus_tag="ACGEUI_001374"
     CDS             11141..12406
                     /gene="purD"
                     /locus_tag="ACGEUI_001374"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFF8166203.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDL
                     VNIVVSEHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPTAQDHKRAF
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVEMIVRPVLEGMVAEGRPYLGVLYVGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPFDYEKGVPLPEKTDGDIITYYAGVKFSENSELLLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIRE"
     gene            12684..13175
                     /gene="purE"
                     /locus_tag="ACGEUI_001375"
     CDS             12684..13175
                     /gene="purE"
                     /locus_tag="ACGEUI_001375"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFF8166204.1"
                     /translation="MKTPIISIIMGSKSDWATMQKTAEVLDNFGIAYEKKVVSAHRTP
                     DLMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDLLYS
                     IVQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSG
                     ELI"
     gene            13162..14235
                     /gene="purK"
                     /locus_tag="ACGEUI_001376"
     CDS             13162..14235
                     /gene="purK"
                     /locus_tag="ACGEUI_001376"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFF8166205.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPASDSPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSACQLPQGTDLLRISQNR
                     IVEKDFLANKAGVTVAPYKVVTSSLDLGGLDLTKTYVLKTETGGYDGHGQKIIRSAED
                     LPEAQQLANSAQCVLEEFVNFDLEISVIVSGNGKDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKKTAVQIAKKLQLSGTLCVEMFTTADDIIVNEIAPRPHNSGRYSIEAC
                     DFSQFDTHILGVLGAPLPQIQLHAPAVMLNVLGQHVQQATDYVAKNPSAHLHMYGKLE
                     AKHNRKMGHVTVFAKDADEVKEF"
     gene            14262..>14626
                     /locus_tag="ACGEUI_001377"
     CDS             14262..>14626
                     /locus_tag="ACGEUI_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HFF8166206.1"
                     /translation="MANIQINRIKSKLTELFSSIIYMGNIKVDEDSEEYKKMWYSRAY
                     SAYSTFLLGAENVDEATKSVTDGFADNGIDAIYNDKNKKILYFIQTKFSNEANGSISE
                     GDTLKFIKGVKKITTSDFS"
BASE COUNT         4183 a         2885 c         3427 g         4131 t
ORIGIN      
        1 ttaaatgaaa acagaaagaa aggcgaataa tcaagtgact aaccagctta tttataaagg
       61 aaaagcgaaa gatatttata gtacaaaaga tgaaaatgtg attagaacgg tctataaaga
      121 ccaagctact atgttgaatg gagctcgtaa ggagactatt gatggcaagg gagcactaaa
      181 taatcagatt tcatctctta tttttgagaa attaaataag gctggtgtgg tgacacatta
      241 tattgagcaa atttcaaaaa acgaacaatt gaataaaaaa gttgatatta ttccacttga
      301 ggtggtgtta cggaatgtga cagcaggctc attttcaaaa cgttttggag tggaagaagg
      361 acatgttctt gaaacgccaa ttgtggaatt ttactacaaa aatgatgatc tggatgatcc
      421 ttttatcaat gatgagcatg tgaagttttt gggtattgtt aatgatgagg agattgctta
      481 tctgaaagga gagactcgcc gtattaatga actcttgaaa ggttggtttg ctcagattgg
      541 gcttaacttg attgacttca agttggagtt cggatttgat caagaaggta caattatctt
      601 agcagatgaa ttttcgcctg ataactgtcg cctatgggat aagaatggta accatatgga
      661 caaggatgtt tttagaagag accttggcaa cctgacggat gtctatcaag ttgtacttga
      721 gaagttgata gcattgtaac tagaagagga aggaattgaa tccgttcggg aaactccgaa
      781 aaaaagatgg aacttactga aatctcaatt tcagatgtcc tcctattttc cagtcgctcc
      841 cttgtcggac ttggtatctt atatgaataa acgtattttt gttgagaaaa aggctgactt
      901 tggtattaaa tcggctagtc ttgtgaaaga gttgacgcat aatctacaac tgacctcttt
      961 gaaggctttg cgtattgtgc aggtctatga tgtcttcaat ttggctgagg atttgctggc
     1021 gcgtgctgag aagcatattt tctctgagca ggtgacagac tgtcttttga cggaaactga
     1081 aatcactgcg gagcttgata aggttgcctt ctttgccatt gaggcgcttc ctggtcaatt
     1141 tgaccaacgt gctgctagtt cgcaagaagc tttgctatta tttggaagtg acagtcaggt
     1201 taaggtcaat acagcccagc tatacttggt caataaggat attacagaag cagagcttga
     1261 agccgttaag aactatcttt tgaaccctgt tgattcgcgt ttcaaggaca ttactttgcc
     1321 gcttgaagag caggctttct ctgtatctga taagacgatc cctaatcttg atttctttga
     1381 aacttatcaa gctgacgatt ttgcgactta taaggcagag cagggcttgg ctatggaggt
     1441 cgatgacctt ctcttcatcc aaaattattt caaatcaatc ggatgtgtgc caactgagac
     1501 tgagttgaaa gttttggata cttactggtc agaccactgc cgtcacacaa cctttgaaac
     1561 tgaattgaag aacattgatt tttcagcttc taaattccaa aaacaattgc agacaactta
     1621 tgacaaatat atcgccatgc gtgatgagct tggtcgttct gaaaagccac aaacacttat
     1681 ggatatggcg actatttttg gtcgttatga gcgtgccaac ggtcgtctgg acgatatgga
     1741 agtctcagat gaaatcaatg cctgctcagt tgagattgaa gtagatgttg atggtgtgaa
     1801 agagccttgg ctcctcatgt ttaagaacga gactcacaat cacccaacag aaattgagcc
     1861 attcggtgga gcggcgactt gtatcggtgg tgctattcgt gacccattgt caggacgttc
     1921 atacgtttat caggctatgc gtatttcagg cgcaggcgat atcacgactc cgattgcgga
     1981 aacacgtgct ggtaaattgc cacaacaagt tatttctaaa actgcggcgc acggctattc
     2041 ttcatatggt aaccaaattg ggcttgcgac aacttatgtg cgcgagtact tccaccctgg
     2101 cttcgtagcc aaacgtatgg agcttggagc tgtggttggt gctgcaccta aggaaaatgt
     2161 ggttcgtgaa aaaccagaag caggcgatgt ggtcatcttg ctcggtggta aaacaggtcg
     2221 tgatggtgtc ggtggtgcga caggttcatc taaggttcaa acggttgaat ctgtggaaac
     2281 agctggcgca gaggtacaaa aagggaatgc cattgaagaa cgtaagattc aacgtctttt
     2341 ccgtgatggc aatgtcactc gtcttattaa gaaatcaaat gacttcggtg caggtggtgt
     2401 ctgtgttgcc atcggtgaat tggctgacgg tcttgaaatc gatttggaca aggtgcctct
     2461 taaataccaa ggtcttaatg gtactgaaat tgcaatctca gaatctcaag agcgtatgtc
     2521 agtcgttgtt cgtccaaatg atgtggatgc cttcatcgca gcctgcaaca aggaaaatat
     2581 cgatgcagtc gttgttgcga ccgttactga aaaaccaaat cttgtcatga cttggaatgg
     2641 cgaaatcatc gttgatttgg aacgccgttt ccttgatacc aatggtgtcc gtgtcgttgt
     2701 tgatgctaaa gtcgttgaca aggacttgac agttccagaa gcacgcacaa catcagcaga
     2761 gacacttgaa gcagatacgc ttaaggtctt gtctgacctc aaccacgcta gtcaaaaagg
     2821 tcttcaaact atctttgact catctgttgg tcgttcaacc gttaaccacc caatcggtgg
     2881 tcgttaccaa atcacaccga cagaaagttc tgttcaaaaa ttgccagttc aacatggtgt
     2941 gacaacaact gcatctgtta tggctcaagg ttacaatcct tatattgcag agtggtcacc
     3001 ttatcacggt gctgcctatg ctgtcattga agcgacagct cgcttggtag caacgggtgc
     3061 tgactggtct cgtgcacgtt tctcttacca agagtacttt gagcgtatgg ataaacaggc
     3121 agagcgtttt ggtcagccag tatcagctct tcttggttct attgaggctc agattcaact
     3181 tggtttgcca tcaatcggcg gtaaggactc tatgtctggt actttcgaag acttgacagt
     3241 accaccaacc ttggtagctt tcggcgtgac aacagcggac agccgcaagg ttctctctcc
     3301 tgagtttaaa gcggctggcg aaaacattta ctatatccca ggtcaagcta tttcagaaga
     3361 tattgatttt gaccttatca aggataactt tagccagttt gaagctattc aagctcaaca
     3421 taagattaca gctgcctcag ccgctaaata cggtggtgtc ctagaaagtc ttgctctcat
     3481 gacttttggt aaccgtatcg gtgcttctgt tgaaattgca gagcttgaca gcagcttgac
     3541 agctcaactc ggaggttttg tctttacatc agctgaggaa attgctgacg cggtgaaaat
     3601 cggtcaaact caggcagact ttacagtcac tgtcaatgga aatgaccttg ctggcgctag
     3661 ccttctagca gccttcgaag gcaaattgga agaggtttac ccaacagaat ttgagcagac
     3721 agatgttctt gaagaagttc ctgctgtggt atcagatact gttatcaagg ctaaggaaac
     3781 aattgaaaaa ccagtggttt acattccagt cttccctggt accaactcag aatacgattc
     3841 agctaaggcc tttgaacagg ttggagctag tgtcaacttg gtaccatttg taaccttgaa
     3901 tgaggttgct attgctgagt cagttgacac tatggttgct aatattgcta aggcaaatat
     3961 catcttcttt gctggaggtt tctcagcagc ggatgaacca gatgggtctg ctaagtttat
     4021 cgtcaatatc ttgcttaacg agaaggtccg cgcagctatt gacagcttca tcgaaaaagg
     4081 tggccttatc atcggtatct gtaatggttt ccaagccctt gttaaatcag gtcttcttcc
     4141 atacggaaac ttcgaggaag ctggtgagac aagtccaact ctcttctata atgatgctaa
     4201 tcagcatgtt gccaagatgg ttgagactcg tatcgcaaat accaactcac cttggttggc
     4261 aggagttgag gtcggcgata ttcatgccat tccagtttca catggtgaag gtaaacttgt
     4321 tgtcagcgct tctgaatttg cagagctaag agacaatggt caaatctgga gccaatatgt
     4381 ggactttgac ggacaaccat ctatggattc taaatacaat ccaaacggct ctgtcaatgc
     4441 catcgaaggg attaccagca agaatggtca aatcatcggt aagatgggac actcagaacg
     4501 ctgggaagac ggactcttcc aaaatatccc tggtaacaaa gaccaaatcc tctttgcaag
     4561 tgctgtaaaa tactttacag ggaagtaaaa aggttcagtg aaccttttta gccctcgcct
     4621 tgaattagtg aagggcgagg ttagaaagaa tctcgcttaa tttttgagaa atagtaaaca
     4681 cgggcgataa gttgtgcgaa aaactttcta ttttcgcttt acttgacagc ctttgtatcg
     4741 aggtaaaaat gacatacgaa gtaaaatctc taaatgaaga atgtggagtc tttggtatct
     4801 ggggacatcc tcaggcagct caagtcactt actttgggct tcatagcctt caacatcgcg
     4861 gtcaagaagg agctggtatt gtttcaaatg acaatgggaa actctatggt tatcgaaatg
     4921 ttgggctcct ttctgaagtt tttaagaatc aatctgaatt agataattta actgggaatg
     4981 cggctattgg acatgttcgg tatgctactg caggttctgc agatattcgc aatattcagc
     5041 cttttcttta taaatttcat gacgggcaat ttgctttatg ccataatggt aatttgacaa
     5101 atgctatttc cttaaggaaa gaattagaaa agcaaggtgc aattttcaat gcctcctcag
     5161 atactgaaat tttgatgcac ttgattcgtc gaagccataa ctcaagtttc atgggaaagg
     5221 taaaagaagc tctaaataca gtaaagggtg gctttgccta tctactgatg acagaaaata
     5281 aactcattgc tgctcttgac cctaatgcct ttcgtccttt gtcaattgga caaatgcaaa
     5341 atggtgcctg ggttatttcc agtgagacct gtgcttttga ggtggtaggc gcaaaatggg
     5401 ttagagatgt tgaacctggc gaagttattc ttatagacga tcgtggtatt caatgtgatc
     5461 gttatactga tgaaacgcaa ctggcaattt gttcgatgga atatgtctat tttgcaaggc
     5521 cggattcaac tatacatggt gttaatgttc atacggctcg aaaaaatatg ggaaagcgtc
     5581 ttgcacaaga atttaaacag gatgctgata ttgtaattgg tgtcccaaat tcgtccttat
     5641 cggctgctat gggctttgct gaagaatccg gattaccaaa tgagatgggt cttgtaaaaa
     5701 atcagtatac gcagcgaacc tttattcaac cgacacaaga attaagggaa caaggtgttc
     5761 gaatgaaact atcagcggta tccggtgttg tcaaaggaaa gcgcgttgtt atgattgatg
     5821 actcaattgt aagaggaacg acttctagaa ggattgtcgg attattaaga gaagcaggag
     5881 ctagtgaagt acatgttgct atagctagtc cagaattaaa gtatccttgt ttttatggta
     5941 ttgatattca gactcgtcgt gagttgattt cagccaatca ctctgttgat gaagtgtgtg
     6001 atattattgg cgcagatagt ctgacctatc tctcactgga tgggttgatt gaatcaattg
     6061 gactcgaaac aaaagcacca aatggtggtt tatgcgtagc ttactttgat gggcattatc
     6121 caacaccgct ttatgattat gaagaagaat acctcagaag tttagaagag aaaacaagtt
     6181 tttatattca aaaagtgaaa taagctctgc taattatata aggagaaaac atgtctgaaa
     6241 aaaatgctta tgcaaaatct ggtgttgatg ttgaagctgg ttatgaagtg gttgaaagaa
     6301 taaaaaaaca cgttgctcgt acagaacgtg cgggagtcat gggagctcta ggtggctttg
     6361 gtgggatgtt tgacttgagt aaaacaggag ttaaagagcc tgtcttggtt tcagggactg
     6421 acggtgtcgg aacaaaactt atgcttgcta tcaagtacga caagcacgac acaatcggtc
     6481 aagactgtgt tgccatgtgt gtcaatgata ttattgcagc aggtgctgag ccattatatt
     6541 ttttagatta tattgcgaca ggaaaaaata acccagtcaa acttgaagaa gttgtttctg
     6601 gtgttgcaga aggttgtgtt caagcaggag cagcactcat tggaggtgag accgctgaaa
     6661 tgcctggaat gtatggtcaa gatgattatg accttgcagg ctttgctgtt ggtgtggctg
     6721 aaaaatctca aatcatcgac ggttcaaaag taaaagaagg cgatattctt ttgggacttg
     6781 cttcaagtgg tatccattca aacggttatt ctttggtacg tcgtgtcttt gctgactata
     6841 ctggtaaaga gctgcttcca gagcttgaag gcaaacaact taaggatgtc ctccttgagc
     6901 caactcgtat ctatgttaaa gcagctctgc cattaatcaa ggaagaacta gtcaaaggta
     6961 tcggccacat cacgggtggt ggctttatcg agaatattcc tcgtatgttt gcggatgatt
     7021 tggctgcgga aatcgatgag gacaaggtgc cagtgcttcc gattttcaag gcgcttgaaa
     7081 aatatggtga catcaagcac gaagaaatgt ttgaaatctt caatatgggt gtcggtctta
     7141 tgctagctgt tagtcctgaa aatgttaacc gcgtcaaaga gctcttggat gaaccagttt
     7201 atgaaatcgg tcgtatcatc aagaaagcag acgctagtgt ggtgattaaa taagtcacta
     7261 aattttataa gaaaggagtg tttagttagt ttgcctaact gaacacggga ttaaatttct
     7321 aggaaaaaag ataaaatgac ttagacgcaa gcgtcgttgt tcgttttcct atttttctac
     7381 gaaatttttc ggcaagccga atcatccctt tgtatcgaat atgaaaatcg ctgtttttgc
     7441 ttctggtaat ggttccaact ttcaggtcat agcagagcag tttccagtta gttttgtctt
     7501 ttcagatcat cgtgatgcct atgttttaga gcgtgctcag aatttagcca ttccaagctt
     7561 cgcttttgaa ctcaaggagt ttgagaataa ggtggcttac gagcaagcca tcgttgattt
     7621 gttggacaaa cacgagattg acttagtctg tctggcaggc tatatgaaga ttgttggaga
     7681 aaccttactc ttggcctatg agaggcgtat tatcaatatt cacccagcct acctgcctga
     7741 atttccaggt gcccacggta tcgaggatgc ttgggaagct ggtgttgacc agtctggcgt
     7801 gaccatccac tgggttgact ctggtgtgga taccggtcag gtcatccaac aagtgcgcgt
     7861 gccacgccta gcagacgata gcctagaaag ctttgaaacc cgtatccatg aaaccgaata
     7921 tcaactctac ccagctgtct tagatagctt gggagtggag aggaaagtaa tctagccttt
     7981 atcttaaaat tttaggtaaa gttaggaaag atagtaaaga agaattaacg ttccaaaaag
     8041 aaaggaattg aacccgtctg gaaggcacag aaaaaagata aacatctcct aggcgcaagc
     8101 gtcttcgtcg agtttcctat ttttccttgg ccttctagtc ggacttggta tcttaaatat
     8161 gactaaacgt gctttaatct cagtgtctga caagtcagga attgttgact ttgcaaaaga
     8221 attgaaaaac ttgggttggg atattatctc aactggtggg actaaggtta cccttgacga
     8281 tgctggtgtt gagaccattg ccatcgacga tgtgactaga ttcccagaga tgatggacgg
     8341 tcgtgttaag accctccacc caaacattca cggtgggctt ttggctcgtc gcgacgctga
     8401 cagccacctt caggctgcta aggacaacaa tatcgagctg attgacctcg tggttgtcaa
     8461 cctctatccc ttcaaggaga ctatccttcg tccagacata acctatgatt tggcggtgga
     8521 aaacatcgac atcggcggtc catcaatgct tcgctcagcc gctaaaaatc acgctagcgt
     8581 gaccgttgtg gttgacccag cggattatgc gactgttttg ggagaattgg ctgacgctgg
     8641 tcagacgaca tttgaaacgc gtcaacgctt ggcagctaag gtctttcgtc acacagcagc
     8701 ctacgacgct ttgattgctg agtacttcac aactcaagtg ggagaggcta agcctgaaaa
     8761 attaaccatc acttacgacc ttaaacaggc tatgcgttac ggagaaaatc cacaacaaga
     8821 cgctgatttc tatcaaaaag ccttgccaac agactactca attgcttctg ctaaacagct
     8881 caacggaaaa gaattgtctt tcaataatat ccgtgatgcc gatgcggcaa tccgtattat
     8941 ccgcgatttc aaagaccgtc caactgttgt tgctctcaaa cacatgaacc catgtggtat
     9001 cggacaggct gatgacattg agactgcttg ggattacact tataaagctg acccagtttc
     9061 aatctttggc ggaattattg tccttaaccg tgaagttgac gcagcgacag ccaagaagat
     9121 gcaccctatc ttcttggaaa tcatcatcgc accatcatac tcagaagaag cgctagctat
     9181 tctcacaaat aaaaagaaaa acttgcgaat ccttgagttg ccgtttgatg cccaagctgc
     9241 cagcgaagtg gaagctgagt acactggcgt agttggtgga cttttggtgc aaaaccaaga
     9301 cgttgtggct gaaaatccat ctgactggca agtggtgaca gaccgccaac caacagaaca
     9361 agaggcgact gcccttgagt ttgcctggaa ggctatcaag tatgttaagt ctaacgggat
     9421 tattattact aacgatcaca tgacgcttgg actcggtgca ggtcaaacca accgtgtcgg
     9481 ctcagtcaag attgctatcg agcaggctaa agaccacctt gacggtgccg ttctagcatc
     9541 agatgccttc ttcccatttg cggacaacat tgaagaaatc gctgccgcag ggatcaaagc
     9601 aatcatccaa ccaggtggtt cagttcgtga ccaagactct attgacgccg caaacaaaca
     9661 tggcttgacc atgatcttca caggtgtgag acattttagg cattaataaa aaaatgtggt
     9721 ctaattaaaa ccacattttt tattaaaaga aaatataagt taactataat ttgttagctt
     9781 tatcagcatt tacccatgtt ccacgagatc cgatttgaac ataaatacca ttacttggtt
     9841 gatcaatttt taatacttta taactaccag tgacgataaa atattcaccc acacggagaa
     9901 tttgatctcc taaaactttt ccttggttat ctgtttcatc taatggtgtg ggatcaatcc
     9961 agtttagtgg agttgggtct cctccggcta attctttatt aacgatcaaa ttattaacaa
    10021 gctgatctac acgaaaaacg ccagggaagg taacagtatc accgacctta atggaaggtt
    10081 ttgtattgtc attttgaacc acagactgtg cgggctcttt aatgggaata tagcgtcggt
    10141 ttccagaaaa actgaggtag ctaagccatg tataacctcc agcagtcacg actttatcgt
    10201 aataaacaga ttgacctgct tcatagtaag caagatcagg gctatcgata ctggtttgtc
    10261 ctttgactgg taatctggtt gtaaagtgat aagttccaga ggggtcaaat gaagcttgag
    10321 aaatgtgttt tagttgtctt gggtaggaat gacttgtctg agatgataag tctttaaaat
    10381 ggatataacc acttacctga gattttggaa tttgacgctt atggtatctt tcagggcctt
    10441 gtccagcgtt gtaattatac tcttcgatag tgacagtgtc tccacggata tcagctaccc
    10501 atgctacatg accgtaagca gcatttgact gataagcgtt tttatcaaac caagcgatag
    10561 cccctatgct tggtgtctta ttcacaggat aaccctgatt tttcgcgata tgtccccacg
    10621 tgcaggcatt accgtagcct ttaggtaact gaaaaccatt agcagagctt aaacgaaaag
    10681 ctgcaaaaga agtgcattgg cggatataca tgttccacga atcgattcca ttgccttttt
    10741 tccacttact cggatagtta tcgcctaaaa cagctgcatg aacaagattt tcctgttgcg
    10801 aaataagtgt agatggcata gtaggtacta gaccattaaa acctaaaagg attactcctg
    10861 agaccaaaaa acgatgaaat tttttcatta attacctcct gtaacttatt attcgaaagc
    10921 aagaaacaaa aaatgttatt tttagatgat tattttgaaa attggcttta cttgatatta
    10981 aataactgtt tgattggcta atagagtgtt ccttatttca agaattgtgg tctactatca
    11041 catttttaca atgtttcaat tattgataac taatacatta tgtaaaaaag cgaacgttta
    11101 tgatataata tctaattata atacgaaaga ggtggatgtt ttgaaattac ttgttgttgg
    11161 ttcaggtggt cgtgaacatg cgattgctaa gaagttgtta gcgtctaagg gtgtggatca
    11221 ggtttttgtg gcacctggta atgatggtat gaccttggat ggtctggact tggtgaatat
    11281 agtagtttcc gaacattcca ggttgattgc ctttgctaag gagaatgaga tttcttgggc
    11341 ctttattggt cctgatgatg cgctagcagc tggtatcgtt gatgatttca acagtgctgg
    11401 actcagagct tttggtccaa caaaggcagc agcggagcta gagtggtcaa aagactttgc
    11461 caaggaaatc atggtcaaat acaatgtccc aacagcagcc tatggcacat tttcagactt
    11521 tgaaaaagcc aaagcctaca tcgaagagca gggcgcacct atcgtggtca aggctgatgg
    11581 cttggcgcta ggtaagggtg tggtcgtggc tgaaaccgtt gagcaggcgg tagaggcggc
    11641 gcaagagatg cttctggaca ataagtttgg cgattcagga gcgcgtgtgg ttatcgagga
    11701 attcttggat ggcgaggagt tttccctctt tgcctttgct aatggcgata agttctacat
    11761 catgccgaca gctcaggatc acaagcgtgc ctttgacgga gacaaggggc caaacactgg
    11821 tggtatgggt gcctatgcgc cagttcctca tctacctcag agcgtggtag acacagcggt
    11881 tgagatgatc gttaggcctg tccttgaggg catggttgcc gaagggcgtc cttatctagg
    11941 tgtcctctat gtggggctta tcctgacagc tgatggacct aaggttatcg agttcaactc
    12001 acgtttcggt gaccctgaaa ctcagattat cctccctcgc ctgacttctg atttcgctca
    12061 gaatattgac gatatcatga tgggcattga gccttacatc acttggcaga aggacggtgt
    12121 gactttgggt gttgtcgttg cctcagaagg ctatccattc gattatgaga aaggtgtgcc
    12181 actgcctgaa aagaccgacg gcgatatcat cacctactac gcaggagtta agttttcgga
    12241 aaatagcgag ctacttctct caaacggagg acgtgtctat atgctcgtca ccacagaaga
    12301 cagcgtcaaa gcagggcagg acaaaatcta tacccaactg gcccaacaag acacaacagg
    12361 tctcttctac cgaaatgata tcggaagcaa agctataaga gaataagact aaccattcaa
    12421 atattagcgt cagcaatcag cgaccgaagg tcgctccaaa aagaaagaag aaaggaactg
    12481 aacacgggac taatttccca ggaaaaaaat aaaactacct ggacgcaagc gtcatcgtta
    12541 gttttctgcg tcgtaggcgt ctgatgctaa agcacctagt cgccctgacg tgggttcgtc
    12601 ttcgaaatca aagatttcgg acttacctca tatttttcta cggaaagttt cgacaagtcg
    12661 aaacgcccct ttgtatctta attatgaaaa ctccaattat ttctattatc atgggctcca
    12721 aatctgactg ggcaaccatg caaaaaacgg ctgaagttct agacaacttt ggcattgctt
    12781 acgagaagaa agtcgtctct gcccaccgta cgccagacct catgttcaag catgctgagg
    12841 aagcacgtgg tcgtggcatc aaaatcatca tcgcaggagc aggtggggca gctcacttgc
    12901 ctggtatggt ggcagctaaa acaacgctgc ctgttatcgg tgtgcctgtc aaatcacgtg
    12961 ccctttcagg tttggatttg ctatattcca ttgtgcagat gcctggcggt gtgcctgtgg
    13021 caaccatggc tatcggagaa gcgggagcga ctaacgctgc cctgacagcc ctccgtatcc
    13081 tctctatcga ggaccaaaat ctggctgatg cgctagctca tttccatgag gaacaaggaa
    13141 aaatcgcaga ggagtcaagt ggtgaactca tttaagacca ttgggattat cggtggtggt
    13201 cagctggggc agatgatggc cattgcggct atctatatgg gtcacaaggt catcacgctg
    13261 gatcctgcta gcgacagccc tgcctcccgc gttagcgagg taattgtggc gccttacgat
    13321 gatgttgagg ctttgggaca attggctgcg cgttgcgatg tgttgactta tgagtttgag
    13381 aatgttgatg cggacggttt ggatgccgtt gtgtcagctt gtcagctacc gcaggggacc
    13441 gatctgctcc gcatttccca aaaccgtatc gttgaaaaag actttctggc aaataaggct
    13501 ggtgtgacag tcgctcccta taaggtggtg acgtctagcc ttgacctagg ggggcttgat
    13561 ttgaccaaga cctatgtcct caagacagag acaggtggtt atgacggtca tgggcaaaag
    13621 attatccgct cagcagaaga tttgccagag gcgcagcaat tagctaactc agctcagtgt
    13681 gtcttggaag agtttgtcaa ctttgacctt gaaatctcag tcatcgtgtc tggaaatggc
    13741 aaggatgtga cggtctttcc cgttcaggaa aatatccacc gcaacaatat cctgtcaaaa
    13801 accatcgtac cagctcgcat ctcagatcaa ctggctgata aggctaagaa aacggcggtg
    13861 cagattgcca agaaactcca gctgtcagga actctctgtg tggaaatgtt tacgaccgca
    13921 gatgatatca ttgtcaatga aattgcccca cgcccccaca actcagggcg ctattctatc
    13981 gaagcctgcg acttttcaca gtttgacacc cacatcttgg gtgtgttggg cgcaccactt
    14041 cctcagattc agctccacgc cccagccgtc atgctcaatg tcctaggaca acatgtccag
    14101 caggcaactg actatgttgc caaaaaccct agcgcccacc tccacatgta tggtaaacta
    14161 gaagcgaaac ataaccgcaa aatgggacat gtgacggtgt ttgcaaaaga tgcggatgag
    14221 gtgaaggagt tttaaagagt taattaggag atgtttattt tatggcaaat attcaaataa
    14281 atagaataaa aagtaaattg acagaactgt ttagtagtat tatctatatg ggtaatatca
    14341 aagttgatga ggactctgaa gagtataaaa aaatgtggta tagtagggct tactcagctt
    14401 attcaacttt tctacttgga gcagaaaatg tagatgaagc aactaagtca gttacggatg
    14461 gatttgctga taatggcata gatgccatct ataacgataa aaataaaaaa attttatatt
    14521 ttattcaaac taaatttagc aatgaagcaa acggaagcat ttctgaaggt gatacattaa
    14581 aatttattaa aggggttaaa aaaatcacca cttcagattt ttcaaa
//
LOCUS       DAXWIQ010000041        14012 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000041 DAXWIQ010000000
VERSION     DAXWIQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14012
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.041"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(179..2194)
                     /gene="recG"
                     /locus_tag="ACGEUI_001378"
     CDS             complement(179..2194)
                     /gene="recG"
                     /locus_tag="ACGEUI_001378"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFF8166207.1"
                     /translation="MILTAPMSNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
                     IDAHLELKENLPATLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENKSETNGLPILYSKRAMETKISSLPFILTNAQKRSLDDILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIMTRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     YKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARKVSAAIVSDPNWIYEKQWQLVAQNIRKKEVYD"
     gene            complement(2569..4023)
                     /locus_tag="ACGEUI_001379"
     CDS             complement(2569..4023)
                     /locus_tag="ACGEUI_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8166208.1"
                     /translation="MERLGGADLDYCYHNHHDTAMISAEQLVFTYHDQKNPACQISTC
                     QIASGQFIVLCGPSGSGKSTFLKLLNGIIPDYYAGKYEGRLDVADCQAGRDSVETFSR
                     SVASVFQNPASQFFYREVQHELVFPCENQGLDAKVIMKRLWTLAEDFAFAELLNKDMF
                     GLSGGQKQRVAIATAIMQGTNIMLFDEPTANLDSAGIAAVKAYLTQLKAAGKTIIVAE
                     HRLHYLMDLADNFFYFKNGRLTDKLTTQNLLALTDEQRQDMGLRRLDLSDLKPVLAGK
                     IESQHYRPDDSLCIEHLTVRAGSKILRCIEQLSFAVGSISGITGSNGLGKSQLVYYIA
                     GILDDKKATIKFQGIPLSAKQRLSKTSIVLQEVSLQLFAESVSKEVNLGHERHPRTTE
                     VIERLSLTTLLERHPASLSGGEQQRVMIAASLLADKDILIFDEPSSGLDLLQMKALAN
                     LLMQLKTQHKVVILISHDEELLSSVCDTIYQMLP"
     gene            complement(3960..4640)
                     /locus_tag="ACGEUI_001380"
     CDS             complement(3960..4640)
                     /locus_tag="ACGEUI_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFF8166209.1"
                     /translation="MRLDVRTKLLLLVLANACFFFRVDGFLEFIIVIFLLLLLSALNK
                     KKLAFKLAVVYLLMIGLSVIPLSIFPSYLDHLLSFVSIAGRLVFPSLLAGLITIKTTT
                     IYELVHGLRKWRFPEVWLLTLAVMCRFIPMIRQECCVIHRSLKIRGIILTKWSILIRP
                     KQYLEYLMVPLLLSLIRSSQELTIASLTKGLAVNKGTSECFSSHLTWKDWGVQIWITV
                     IIIITILQ"
     gene            complement(4637..5230)
                     /locus_tag="ACGEUI_001381"
     CDS             complement(4637..5230)
                     /locus_tag="ACGEUI_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFF8166210.1"
                     /translation="MKQLTIKDIMITGAFAALYFICVGLGTFINIFFDRSGNMLYAPA
                     FAALLGGTIYFLLIAKLQTFGPITLLGLMMGGFFFLSGHFFAAMFPSIIFGLIADIIA
                     SLGKYKSRFFNDLSYMVFAFVNSGPILLMWLLKDAYVESLLARGKSMTYVNRIMYDFS
                     LGHVIWFVGIVLAGALLGSVFAHYLLKKHFIKSGLLS"
     gene            complement(5227..6897)
                     /locus_tag="ACGEUI_001382"
     CDS             complement(5227..6897)
                     /locus_tag="ACGEUI_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HFF8166211.1"
                     /translation="MAKPNRSTLSLVWRLLTLMKTLLPWIILAVGFAVMGFVITVSIP
                     TGIAYLGLLAIRQEVIPILALYLLIALAFLRGFVRYGEHYFGHFVAFHSLAAFRNLIF
                     KKLRALSPACLDSQDSGYLLKMIGEDIEALEVFFAHTIAPICTAILSAGLMFWYFCQS
                     SWQLALLALATYACLAIVIPIYFANILQVLLKSQNEGRKDYLSYFLESLRSVKDLLQF
                     QVLDEQFERLIKKSNHVNALDRNVAQAQFLQMALTFFWLGLMILAFSYMVFDGICHDS
                     LSFDKGLLTFIAFTASFSPFLELGRLPLGFKRAMNAARNIFDLLDENVIVDEGTKQID
                     RLRSVSFEDLSFAYPKRQELVFKDLTVTFQEKGIIGIKGESGSGKSTLVKLIMKWYNW
                     KTGDIFLNDRNSCLLNAAKLQDTIAYVPQTAQLFQQSIRENLIFGRQDISDESIWNLA
                     EACGMKDRLLACKEGLDTIIKSPSDFSAGEGQRLELMRALLKDASCYIFDEPTSNLDS
                     LNEAILLDLIKTHCQGLVFLISHRPSTLACVDHLFCVKNGSLKEVNKK"
     gene            complement(6890..8653)
                     /locus_tag="ACGEUI_001383"
     CDS             complement(6890..8653)
                     /locus_tag="ACGEUI_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFF8166212.1"
                     /translation="MMETEDTISRGKRKRLLKRLRERIAPKRYLLYVSAFLSWLQFVM
                     RMISFYLIAKTFSTFILGHAIALGRLAGLLLLLNVVGFVLAILGKQLQGIASQFARDS
                     LKQSFFEAFIDLDGQFDAHASDADILTLASQGIDSLDTYYGYYLSLSMRTKWNCTTIM
                     ILVFLIYPLAGLVFLGVLPLIPLSIVAMQKRSKQIMSHYWSSYMDVGNLFMDDLKGLN
                     TLYSYQATERYEQEFSGKAEQFRKATMSLLGFQLQAVGYMDAVMYLGIGLSGFLAVQA
                     LATGQLSFFNFLFFLLIATEFFTPIREQGYGMHLVMMNTKMADRIFSFLDSVPARKDN
                     KSKTAINFNQIDIQNISLAYEKKTVLSGVTMTLTKGQLTAIAGVSGQGKTSLAQLLLK
                     RQSATTGHILFDGLDSDNLSQETINQQVLYVSDQSTLLNRSIYDNLRLAANLSKKEIL
                     DWIDQHGLLSFINWLPDGLDTIVGENGNLLSPGQKQQVICARALLSKRSLYIFDEATS
                     SLDAENERIIDNLITRLAKTAIVIVITHKMSRLKGANQVLFLNTGQPACLGKPCDLYR
                     DQPTYRHLVDTQARLEASIYG"
     gene            complement(8650..9486)
                     /locus_tag="ACGEUI_001384"
     CDS             complement(8650..9486)
                     /locus_tag="ACGEUI_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8166213.1"
                     /translation="MSKNSSHIQVQNLSFAYEEKLVLDDLTFSIPKGKITTIMGANGS
                     GKSTLLQLLTKNLPLKQGQVWLEQEELATISLKAFAKKVAVVHQYHQVVEGLLVGELV
                     AMARLARRSFFKTTTKEDEERLAWALEVTALSDYVYRDIQHLSGGEQQRVWIAMALAQ
                     DTDIIFLDEPTTYLDIKYQLDILKLITKINQELGLTIVLVLHDINQALSLSDHLIGLK
                     DGRLYAQGQPRDLLDQQFIRDVFGVELPFVERNGQQFVLTSLVNETNGIRTQRRDRNK
                     NR"
     gene            complement(9483..10505)
                     /locus_tag="ACGEUI_001385"
     CDS             complement(9483..10505)
                     /locus_tag="ACGEUI_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFF8166214.1"
                     /translation="MTASDLQRDQSLLAKKRFRSGLYLVLLILSLLFLAVIALSLGGL
                     AVSYGAIVKGLFVAYDPQVALIYDLRFPRIVIALLAGAGIAVSGVLFQAVLKNPISDP
                     AIIGICSGASFMVLVSSLLLPQLLLYGPIVSFLGGGVSFLLIYGLAWKKGLNPIRLIL
                     TGIAINALFMGLSTALTSFFTSASPMVNALLAGHISQKTWADVGVLFPYTFIGLLLAL
                     LLSKTCNLLLLDDQVIRHLGIDATALRLGISLVAVLLASVATSIVGVVSFLGLIVPHM
                     SRLLVGSKHQILIPFSALLGAFVFLLADTLGRSLAYPLEISPAIIMSIVGGPYFIYLL
                     RRSDII"
     gene            complement(10507..11391)
                     /gene="isdE"
                     /locus_tag="ACGEUI_001386"
     CDS             complement(10507..11391)
                     /gene="isdE"
                     /locus_tag="ACGEUI_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HFF8166215.1"
                     /translation="MIKRCKGIGLALMAFFLVACVNQHPKTAKETEQQRIVATSVAVV
                     DICDRLNLDLVGVCDSKLYTLPKRYDAVKRVGLPMNPDIELIASLKPTWILSPNSLQE
                     DLEPKYQKLDTEYGFLNLRSVEGMYQSIDDLGNLFQRQQEAKELRQQYQDYYRAFQAK
                     RKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDLAGGENVYQSDEKEFLSANPEDMLA
                     KEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTAVKEGKVYDLDNTLFGMSAKLNY
                     PEALDTLTQLFDHVGDHP"
     gene            complement(11375..12250)
                     /locus_tag="ACGEUI_001387"
     CDS             complement(11375..12250)
                     /locus_tag="ACGEUI_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding Shp domain-containing protein"
                     /protein_id="HFF8166216.1"
                     /translation="MTKVVIKQLLQVIVVFMISLSTMTNLVYADKGQIYGCIIQRNYR
                     HPISGQIEDSGGEHSFDIGQGMVEGTVYSDAMLEVSDAGKIVLTFRMSLADYSGNYQF
                     WIQPGGTGSFQAVDYNITQKGTDTNGTTLDIAISLPTVNSIIRGSMFVEPMGREVVFY
                     LSASELIQKYSGNMLAQLVTETDNSQNQEVKDSQKPVDTKLGESQDESHTGAMITQNK
                     PKANSSNNKSLSDKKILPSKMGLTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGF
                     AVWVWKKRKKNDKTM"
     gene            complement(12447..>14012)
                     /gene="shr"
                     /locus_tag="ACGEUI_001388"
     CDS             complement(12447..>14012)
                     /gene="shr"
                     /locus_tag="ACGEUI_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein Shr"
                     /protein_id="HFF8166217.1"
                     /translation="FKQNNQLTKLDFFNNLLTQVEESVFPDVETLNLDVKFNQIKSVS
                     PKVRALIGQHKLTPQKHIAKLEASLDGEKIKYHQAFSLLDLYYWEQKTNSAIDKELVS
                     VEEYQQLLQEKGSDTVSLLNDMQVDWSIVIQLQKKASNGQYVTVDEKLLSNDPKDDLT
                     GEFSLKDPGTYRIRKALITKKFATQKEHIYLTSNDILVAKGPHSHQKDLVENGLRALN
                     QKQLRDGIYYLNASMLKTDLASESMSNKAINHRVTLVVKKGVSYLEVEFRGIKVGKML
                     GYLGELSYFVDGYQRDLAGKPVGRTKKAEVVSYFTDVTGLPLADRYGKNYPKVLRMKL
                     IEQAKKDGLVPLQVFVPIMDAISKGSGLQTVFMRLDWASLTTEKAKVVKETNNPQENS
                     HLTSTDQLKGPQNRQQEKTPTSPPSAATGIANLTDLLAKKATGQSTQETSKTDDTDKA
                     EKLKQLVRDHQTSIEGKTAKDTKTKKSDKKHRSNQQSNGEESSSRYHLIAGLSSFMIV
                     ALGFIIGRKTLFK"
BASE COUNT         4498 a         2899 c         2363 g         4252 t
ORIGIN      
        1 ataatatgat tattttagca ataaaaaagt tatactgaaa atgggttaat actgcttcac
       61 atagcagtct catcgtaaag cataagtaaa aaaggccaga aatagtgacc tctttatact
      121 ttataacagt ttatctcaaa actaactaaa tatttgtaaa acaacgactt gttttaagtt
      181 aatcataaac ttcttttttt ctaatatttt gtgccactaa ttgccactgt ttttcatata
      241 tccagttagg atcagaaaca atcgctgcag aaactttgcg tgcttcttct aaaataggat
      301 aatcctcaac gatatcagct acttgaaatt ctggaattcc agactgacga gtaccaaaga
      361 tttcaccaga accacgcatt tttaaatccg actcagctaa aacgaaacca tctgtcgttt
      421 ctgtcatgat tgtcattcgt tttttccccg aatcagtttt gggattagcc actaaaacag
      481 cgtatgattg tttatatcca cgaccaacac gcccacgaag ttgatgtaac tgacttaatc
      541 caaaacgatc ggcatccata ataatcatga tcgttgcatt tgggacattt acccctactt
      601 caataactgt tgtggatact aaaatatgac tttttttatc cttgaaatct tgcattatag
      661 catctttttc atcatttttc atacgtccat gtacaagagc aaccttagca attccttcaa
      721 aataagtaga taattcagca tgcaatgcta ctgcattctt taaatctaaa gcttctgatt
      781 cttcaatcaa cggtgaaatg acatacactt gagcatcttt ttgcaattca ccttttaccc
      841 attccaacac agtacctagc tgctcgtgtt tcacccagcg tgtcataata ggtttacgac
      901 cggcaggtaa ttcatcaata atagaaacat ccatttctcc aaaagctgtg attgctagag
      961 ttcggggaat tggggtggct gtcatcatta aaacatcagg attttctccc ttttcacgga
     1021 aaattctacg ctgtttaaca ccaaaacgat gttgctcgtc tgtaatgaca agccccagtt
     1081 tatggtactg taccgagtct tggataagag catgagttcc tacaatcata tcaaccgagc
     1141 catttgcaat agctgctaaa accgtacgct tgacagctgc tttcataccc gaagttaata
     1201 tagcgattga taaatctgga aataactctt gcagactaat gtagtgttgt tcagctagga
     1261 tttccgttgg aaccatcaaa gccgattgaa aacctgctgt ataagctgca tacattgata
     1321 gaccagcaat gactgtcttt cctgatccta catctccttg caataaacga ttcatatgag
     1381 ctcccgatga catatcagat aatatgtcat ctaaagagcg cttttgagca ttcgttagaa
     1441 taaaaggtaa agaggaaatc tttgtctcca tagcacgttt actataaaga ataggcaaac
     1501 catttgtttc agatttattt tcggctttca aaacttgaag gttcatttga aagtaaaata
     1561 attcttcaaa tttaatccga cggagcgctt gcttatactc tgtgatatct tttgggaaat
     1621 gcatagctaa acaagcctga ctacgaccca tcaatcggta tttttccaat aaagtagctg
     1681 gtaaattttc cttcaattcc aaatgcgcat cgatttcaaa agctgactta atagctttaa
     1741 tcaaagtaga ctgtgaaatt ccctgagcta cgcgataaac aggttgcatg tcatcttcaa
     1801 cttgagctaa aaccttcatc ccagtaatag ccgatttagt ggcatcccat ttaccaaaaa
     1861 cagctacctc ttgaccaagt tctattttat cagctaaata gggttgatta aaaaaactaa
     1921 catttaagac agcttcccct tgacgcaatt tgaaacttaa acggttacgt ttaaaaccat
     1981 aatattgtac attagctgga gtaacgacta agcctgtaat gactgctttt tcaccatcca
     2041 ctaaatcaaa aacagatttt gatttaaaat cttcatagcg aaacggataa taaagcagta
     2101 aatcttctac tgtataaata tctaatttct gaaatttttc tgctgatttt ggtccaaatc
     2161 cctttaagtt ggacatagga gctgttaata tcattatctt tttcccttca caaaaactct
     2221 tgtcagattg aagacaaagt ttgtcgtgat actttcctta ggtggtgctt acggcagcga
     2281 cgttctttgg aattccatgc ctttattttt gagcctgagg tctcaaaaat cccgagtggc
     2341 gtcaatacga gattgacgcc actcttttac cacagcaaaa agcatctgtt ttggctcact
     2401 ttgtgtgtaa aaatcggaaa ccgaatgtca aatcagcaga gctattcatg ctttttgagc
     2461 ataaaactgg ggttgtctac gttctgactc ttgtatctat tatacaagag ttcctattta
     2521 ttttcagata aaagcctttt agtactttta aacagttcta ggggctcgtt aaggtagcat
     2581 ttgataaatg gtatcgcaga cactacttag aagttcttca tcatgtgaaa tgaggatgac
     2641 aaccttatgt tgggtcttca gctgcatgag aagattagct aaagccttca tttgtaacaa
     2701 atcaagtccg ctacttggct catcaaaaat taagatatct ttatcagcca aaagactagc
     2761 ggcaatcatc actctttgtt gctcacctcc tgacaaacta gctggatgac gttctaaaag
     2821 agtcgttaaa gataaccgct ctattacttc tgtcgtccgt ggatgtcgtt catggcccaa
     2881 gttgacttcc tttgaaaccg attctgcaaa tagttgcaaa gaaacttctt gcaagacaat
     2941 actagtcttt gataaacgtt gcttggcaga taaaggtatg ccttgaaact taatagttgc
     3001 ttttttatcg tctaagatac cagctatata ataaactaat tgcgattttc caagcccgtt
     3061 tgatcctgta atgccagaaa tacttcctac agcaaaacta agctgttcaa tacatctaag
     3121 tattttgctt cccgctctaa ctgttagatg ctcaatacac aggctatcat ctgggcgata
     3181 atgttgactc tcaatcttac cagctaacac aggttttagg tcagacaagt ctaagcgacg
     3241 tagtcccata tcttgccgtt gttcatctgt caaggctagc aaattttgtg ttgttaactt
     3301 atctgttaat ctaccatttt taaagtagaa aaagttatct gccaaatcca tcaagtaatg
     3361 taagcgatgc tcggctacaa taatggtttt tcctgctgct tttaattggg taagataggc
     3421 tttaacagca gctatgcctg cactatctaa gttagcagtt ggctcatcaa aaagcataat
     3481 attagtacct tgcataatgg ccgttgcaat agcaactctt tgtttttgcc ctcctgacaa
     3541 tccaaacata tccttgttaa ggagctctgc aaaagcaaaa tcttctgcta aagtccaaag
     3601 acgcttcatg ataacctttg catctaaacc ttgattttca caaggaaaga ctagttcgtg
     3661 ttggacttcg cgatagaaaa actgactggc tggattttga aaaacagagg ccacagatct
     3721 agaaaatgtt tcaacactgt ctctgcctgc ttggcaatca gccacatcta gtctaccttc
     3781 atattttcca gcataataat caggaattat gccattaaga agttttaaaa aggtagattt
     3841 acctgaacca gaaggtccac acaaaacaat gaattgtcca gaggcaattt gacaagtcga
     3901 tatctgacaa gctggatttt tttgatcatg ataggtaaaa acaagttgtt ctgctgaaat
     3961 cattgcagta tcgtgatgat tatgataaca gtaatccaaa tctgcacccc ccaatctttc
     4021 catgttaagt gtgagctaaa gcattcactg gtgcctttgt tgactgctag ccctttagtt
     4081 agactagcga tagttagttc ttggctactt cttatcaaag ataaaagcaa tggaaccatt
     4141 aaatactcca agtattgttt aggtctgatc aaaatagacc attttgttaa aataatcccc
     4201 ctgattttta aagagcgatg aataacacaa cattcttgtc taatcattgg tatgaaacgg
     4261 cacataacgg ccaaggtcaa tagccagact tctgggaaac gccatttgcg taatccatga
     4321 accaattcgt agatggtagt tgtcttaata gtaattaaac ctgctagtaa agaagggaaa
     4381 actaaccttc ctgctattga gacaaaactc aataagtggt caagataaga agggaaaatc
     4441 gatagcggga ttacagatag accgatcatt aacagataaa caaccgccaa cttgaacgct
     4501 agctttttct tatttaaagc agacaaaagt aataagagaa aaataacaat tataaattct
     4561 aaaaagccat caacacgaaa aaagaagcag gcattggcaa gtaccaacaa aagcagtttg
     4621 gtgcgaacat caagcctcat gataacaatc ctgatttgat aaaatgtttc tttagtaaat
     4681 aatgtgcaaa aactgaccct aataaggctc ctgcaagaac aattcctaca aaccaaataa
     4741 catgccctaa actaaaatca tacataatgc gattgacata ggtcatactt ttaccgcggg
     4801 ctaacaaact ttcaacatag gcatctttca acaaccacat cagcaaaata gggccactat
     4861 tgacaaaagc aaaaaccata taagataggt cattaaaaaa ccttgacttg tattttccta
     4921 aagacgcaat aatatccgct attaaaccga aaataatgct gggaaacatc gcggcaaaaa
     4981 agtgaccact aaggaagaaa aaacctccca tcatcaatcc taataatgtt attggcccaa
     5041 atgtttgcaa tttagcaatc aaaagaaaat aaattgttcc ccccagtaaa gctgcaaagg
     5101 caggggcata taacatattt cctgagcgat caaaaaagat gttaataaac gtgcctagac
     5161 caacacaaat aaagtagagc gcggcaaatg cgcctgttat cataatatct tttattgtta
     5221 actgtttcat tttttattca cctcttttag ggaaccattt tttacgcaaa atagatgatc
     5281 aacacaagct agggttgatg gcctgtgaga aattaagaag accaatcctt gacaatgtgt
     5341 tttaattaaa tcaagcaaga tagcttcatt tagagaatct aaatttgacg tgggttcatc
     5401 aaaaatatag caactcgcat ctttcaataa agctcgcatc aactctaaac gttgaccttc
     5461 tccagctgaa aagtcagaag gactcttgat aatcgtatcc aaaccttcct tacaagctaa
     5521 cagtctgtct ttcataccac aagcctcagc taaattccaa atggactcat ctgaaatatc
     5581 ttgacggcca aagataagat tttcacggat agactgttgg aaaagctgcg ctgtttgtgg
     5641 cacataagca atagtatctt gtaatttagc agcatttagt aagcaactat tcctatcatt
     5701 aagaaaaata tctcctgtct tccaattata ccatttcata attaatttca ccaaagttga
     5761 tttaccagat cctgattcgc ctttaatgcc aataatcccc ttttcttgaa aggtaactgt
     5821 taaatcctta aaaaccagct cttggcgctt tggataagca aaactgaggt cctcaaaaga
     5881 aacactcctt aaacgatcta tttgttttgt gccttcatct actatgacat tttcgtctag
     5941 aaggtcaaaa atatttcgag ccgcattcat agcacgctta aaacctaatg gcaaacgtcc
     6001 tagttctaaa aaaggagaaa acgaagcagt gaaagcaata aaagttaata agcctttatc
     6061 aaatgataag ctgtcatggc atatcccatc aaaaaccata taagaaaatg ctaagatcat
     6121 taagccaagc cagaaaaaag tcaatgccat ttgcaaaaat tgggcttgcg ctacgttacg
     6181 atcaagagca tttacatgat tacttttttt tattaatcgt tcaaattgct cgtctaatac
     6241 ctgaaattgc aaaagatctt tgactgatcg taaactctct aaaaaataag acaggtaatc
     6301 ttttcgacct tcattttggc tttttagtaa aacctgtaaa atattagcaa aataaatggg
     6361 tataacaatg gctaaacaag cataggttgc caaagctaat aaggctaact gccaactact
     6421 ttgacaaaag taccagaaca ttaaacctgc agacaatatt gctgtacaaa taggtgcgat
     6481 tgtgtgggca aaaaaaactt ctaatgcctc aatatcttct ccaatcattt taagcaaata
     6541 accactgtct tgactatcca agcaagctgg agaaagagct cttagtttct taaagatcaa
     6601 gttcctaaaa gctgctaaac tatgaaaagc aacaaaatga ccaaaataat gctctccata
     6661 acgaacaaaa cctcttaaaa aggctagagc aatcaaaaga tataaagcta atattggaat
     6721 cacttcttgt ctaatagcta gtagtcctaa ataagctatt ccagttggaa tacttaccgt
     6781 gatgacaaaa cccataacag caaagccaac agccaagata atccaaggta gcagagtttt
     6841 cattagggtt aataaacgcc aaaccaggct taaggtagat ctgttaggtt tagccataaa
     6901 tacttgcctc cagtcttgct tgagtatcaa ctaggtgacg ataagttggt tggtcccgat
     6961 aaaggtcgca aggtttgcct aaacaagcag gctgccctgt gtttaaaaag agtacttggt
     7021 tagctccttt cagtcgagac atcttatgag taatcacaat aactattgct gtcttagcta
     7081 atctcgttat taaattgtca ataatacgtt cattttctgc atctagacta gatgttgcct
     7141 catcaaagat ataaagagac ctcttactta atagagctct tgcgcaaatc acctgttgct
     7201 tttggcctgg tgataacagg ttaccatttt caccaactat tgtgtcaaga ccatctggta
     7261 gccagttgat gaagcttaac aaaccatgtt gatctatcca atctaagatt tcttttttag
     7321 agagatttgc tgctaacctt aaattatcgt aaatgctacg gttcaataag gtagattggt
     7381 ccgatacata aagaacctgc tggttgatag tttcttgcga caaattgtca ctgtctaaac
     7441 cgtcaaataa aatatgccca gtcgttgcag actggcgttt caagaggagt tgagctagac
     7501 ttgtttttcc ttgtcctgac acccccgcga tagctgttag ttggcccttc gttaatgtca
     7561 tggtaacccc actaagcact gtttttttct cataagctaa agaaatattt tggatgtcaa
     7621 tctggttaaa gtttattgct gttttactct tgttatcttt tcttgctgga acactatcca
     7681 agaaactaaa gatacggtca gccattttgg tattcatcat gaccaagtgc atgccataac
     7741 cctgctcacg aatcggtgta aaaaattctg tagcaatcaa gagaaaaaat aggaaattaa
     7801 aaaaagataa ctggcctgtt gccaaagctt gaacggctaa aaaacctgac aaaccaattc
     7861 ccagatacat aactgcatcc atataaccaa ctgcttgtaa ctgaaagcca agcaaagaca
     7921 tggtagcctt tcgaaactgt tctgccttac ccgaaaattc ttgctcgtaa cgttctgttg
     7981 cttgataaga atacagtgta ttaagacctt ttaagtcatc cataaataaa ttaccaacat
     8041 ccatataaga agaccaataa tgactcataa tttgcttaga ccgcttttgc atagcaacga
     8101 ttgaaagtgg aatcagaggc agaactccta aaaagacaag gcctgccaat ggataaatta
     8161 aaaagactag tatcataata gttgtgcagt tccacttggt tctcatagac aaagacaaat
     8221 agtaaccata ataagtatcc aaactatcaa tcccctgtga agccaacgtt aaaatatcag
     8281 catcactggc atgagcatca aattgcccgt ctaaatcaat aaaagcctca aagaaagatt
     8341 gtttgagtga atctctagca aattgtgaag cgatcccttg cagttgcttg cctaatatag
     8401 caagaacaaa gcctaccaca tttaaaagta ggagtaaacc tgccaagcgt cctaaggcaa
     8461 tagcatgacc caaaataaag gtcgaaaacg ttttggcaat gaggtaaaaa ctgatcatcc
     8521 tcatcacaaa ttgtaaccaa gataaaaagg ctgacacata aagcaaatac ctttttggtg
     8581 caattcgttc acgtaatcgt tttaacagtc ttttcctttt tccccttgat attgtatctt
     8641 ctgtttccat catctgttct tgttcctatc tctcctttga gttctaatac cattggtttc
     8701 attgaccaat gaagtgagga caaactgttg accatttctc tcaacaaagg gcaattcaac
     8761 accaaagaca tctctgataa actgttgatc aaggaggtct cttggttgac cttgggcata
     8821 aagtcgccca tctttcagtc caataagatg gtctgaaaga gacaaggcct gattgatgtc
     8881 atgtaatact aacacaatcg ttaggcctag ctcttggttg attttagtaa tcagcttgag
     8941 aatgtcaagc tggtacttaa tatccaaata agtcgttggc tcatctaaaa aaataatatc
     9001 tgtatcctgg gcaagggcca tggcaatcca aaccctctgc tgttcaccac cagaaaggtg
     9061 ttggatatca cgataaacat aatcactcaa agccgttact tctaaggccc aagcaaggcg
     9121 ctcctcatct tctttggtag tagttttaaa aaaagaacga cgggcaagcc tagccatggc
     9181 taccaattca cccactaaca aaccttcgac cacttgatgg tattggtgta ccactgccac
     9241 ttttttcgca aaagccttga gagaaatcgt tgctaattct tcctgctcaa gccaaacctg
     9301 cccttgtttt aacggaaggt ttttggttaa taattgtaat aaggttgact tgccactgcc
     9361 atttgccccc ataatagtgg taattttccc ttttggaatg ctaaaggtca ggtcatctaa
     9421 aacaagtttt tcctcataag caaaggaaag gttttgcacc tgaatatgac tgctgttctt
     9481 actcatataa tatcagacct ccttagcagg taaataaagt aaggcccgcc tacaatactc
     9541 attataatcg cagggctaat ttccaatggg taagcaagac ttcttcctaa agtatcggct
     9601 agcaaaaaga caaaggctcc aagcaaagct gaaaaaggaa ttaagatttg gtgcttactt
     9661 ccaaccagca agcggctcat atgagggaca atcaagccta ggaaagaaac cactccaacg
     9721 atcgaagtgg ctaccgaagc caataaaaca gcaaccaagg aaattcccaa ccgtaacgct
     9781 gtagcatcga ttcctaaatg acgaatcacc tgatcatcta aaagaagaag gttacaagtt
     9841 tttgacaata acaaagctag taacaagcca atgaatgtat aagggaataa aacccctaca
     9901 tcagcccatg ttttttgact aatatgacct gctaaaagag cattgaccat gggactagct
     9961 gaggtgaaaa agctggttaa agcagttgat aaccccataa ataaagcatt aatagcaatc
    10021 cctgttagaa taagccgaat aggattaagc cctttcttcc atgctaaacc ataaatcagc
    10081 aggaaagaca ctccgcctcc taaaaaggat acgattggac catacagcag caactgcggc
    10141 agaagtaaac tagagactaa aaccataaaa ctagcaccac tacaaatccc tataatagca
    10201 gggtctgaaa taggattttt caacactgct tgaaaaagaa ctcccgaaac agctatccct
    10261 gctcctgcta ataaagcaat gacaattctt ggaaaacgca aatcataaat caaagccact
    10321 tgggggtcat aagccacaaa cagtcccttg acaattgccc cataagaaac cgccaaacca
    10381 ccaagggata aggcaataac cgccaaaaat aggaggctga gaattaaaag aactagataa
    10441 agaccacttc tgaaacgttt tttagccagt agggattgat ccctttgtaa atctgatgct
    10501 gtcatgttac ggatgatctc ccacgtggtc aaaaagctgt gttaaggtgt ccaaggcttc
    10561 tgggtagttc aatttagcac tcatgccaaa cagggtattg tccaaatcat agactttccc
    10621 ttccttgact gccgtaaaat gtttccaaat atcattttca gcaaattctt tgtcaaacat
    10681 cacttttacc ttgtctggaa tggcatgagc tgttcgtaaa atcaagtcag gctccttagc
    10741 cagcatgtct tcaggattag ctgatagaaa ttctttctca tctgactgat aaacattctc
    10801 accacctgcc aagtccaaaa gattccctac ataagattgg ttcgtcgcca ccaaataact
    10861 acctggcaag cccataagaa taagcacttt aggctttttc ttccccttac gtttagcttg
    10921 gaaagcacga taatagtcct ggtattgctg gcgcaattct tttgcttctt gttgacgttg
    10981 gaaaaggttc cctaaatcat caatggactg gtacatgccc tcaacacttc gtaagttcaa
    11041 aaaaccatac tcagtatcca atttttgata cttgggttcc aaatcttctt gtaaagaatt
    11101 gggactcaaa atccaagttg gtttcaaaga agcaatcaac tctatatcag gattcatggg
    11161 taaacccaca cgcttaacag catcatagcg tttaggaagg gtatataatt tactatcaca
    11221 aaccccaacg aggtctaaat ttaaacggtc acagatatca accacagcaa ccgaagtggc
    11281 tacaattctc tgctgttcag tctctttagc cgttttaggg tgctgattca cacaagctac
    11341 caaaaagaag gccattaagg ctagaccaat tcctttacat cgttttatca ttttttttcc
    11401 tctttttcca aacccataca gcaaaccctc ctaacatcag aaaaaaagtt ggaaagtagt
    11461 aaatcatgat tgataagtct tttttagacc gaaacttatc ttcttttttt aactctaaag
    11521 aagtcgttaa gcccatctta gaaggaagaa tttttttatc tgatagactc ttgttatttg
    11581 atgagttggc tttaggctta ttctgagtta tcatagcacc tgtgtgagac tcatcttgac
    11641 tttctcctag ttttgtatca acaggctttt gactgtcttt tacttcttga ttttgagaat
    11701 tgtctgtttc agtaacaagc tgggccaaca tattacctga atacttttgt attaactctg
    11761 aagctgacag gtaaaagacc acttctctgc ccataggttc aacaaacatg ctgcctcgaa
    11821 tgatactatt tacagtaggc aaactaatag caatatctag tgttgtccca ttggtatcag
    11881 tacctttttg ggtgatattg taatcaactg cctgaaagct tccagttcct ccaggttgta
    11941 tccagaactg ataattgccc gaataatcag ctaagctcat acgaaaagta agaacgatct
    12001 ttccagcatc agatacttct agcatggcat cactatagac agttccttca accatacctt
    12061 gaccaatatc aaaggaatgc tcccctccgc tatcttctat ctgcccagat atcggatgac
    12121 gataatttct ttgaataata catccataaa tttgaccttt atctgcatac acaagatttg
    12181 tcattgtcga taaacttatc atgaacacta ctattacctg caacaattgc tttattacta
    12241 cctttgtcat atgctcctct tgtctatctt ttcttaaaca tcttatttca ttttttaaat
    12301 gaatcccatt ttataacatc tccaaatgtg aaaaacacac ctatcttgcg actacttcaa
    12361 cctgtatcag gagtcctgtc ggaaataggt atgtttttag ctcttgctga ctagcttttt
    12421 tgttatttca cttggtacta agtttcttat ttaaataatg tctttcgacc aataatgaat
    12481 cccagagcta cgatcataaa gctagataga cctgcaatta agtgataacg agagctactt
    12541 tcttcaccat ttgattgttg attggaacga tgtttcttat cagatttttt agtcttagta
    12601 tcttttgctg ttttaccttc aattgatgtt tgatggtcac gcactaactg cttcaatttc
    12661 tctgccttat cagtatcatc tgtcttagaa gtttcttgag ttgattggcc ggttgctttt
    12721 ttagccagga gatcagttaa gttagcaata ccagtagctg ctgaaggagg acttgtaggt
    12781 gttttttctt gttgacgatt ttgaggtcct ttcaactgat ctgttgaagt tagatggcta
    12841 ttttcttgtg gattattagt ttctttgaca acctttgcct tctctgttgt aaggcttgcc
    12901 cagtctaaac gcataaaaac ggtttgaagg ccagaccctt ttgaaatggc atccatgata
    12961 ggcacaaaga cctgtaatgg cacaagtccg tctttcttcg cttgttcaat caatttcata
    13021 cgcagcactt ttggatagtt ttttccataa cgatctgcca atggtaggcc agttacatcc
    13081 gtgaaataag acacaacctc tgcctttttt gttcgaccaa ctggtttacc agctaaatct
    13141 ctttggtaac catctacgaa atagctcaat tcaccaaggt agcctaacat tttaccaacc
    13201 tttatacctc taaactcaac ttctaaatag gaaacacctt ttttaactac caaagtcact
    13261 cgatgattaa tagccttgtt tgacatggac tcagatgcta agtcagtttt taacatgctg
    13321 gcatttaaat agtaaatacc atcacgcaat tgtttttgat ttaatgctct aaggccgttc
    13381 tcaactaaat ctttctgatg tgaatgtggt cctttcgcca caaggatatc attagatgtc
    13441 aaatagatat gttctttttg agtagcaaat ttctttgtta ttaaagcttt gcgaatccga
    13501 tatgtacctg gatcttttaa agaaaactct cccgttaagt catctttcgg atcatttgag
    13561 agaagctttt cgtcaaccgt cacatactgt ccattggaag cttttttttg caactgaatc
    13621 acaatactcc aatcgacttg catatcatta agtaaagaaa ccgtatctga acctttttct
    13681 tgtaacaatt gttgatattc ttcaacagac actagttctt tatcaatggc agaatttgtt
    13741 ttttgctccc aataatacaa atctaaaaga ctgaaagctt gatgatattt tattttttcg
    13801 ccatctaagg aagcttcaag ttttgcaata tgtttttgtg gagtcagttt gtgttgtccg
    13861 ataagagctc ttacttttgg actcacactt tttatctgat tgaacttcac atctaaatta
    13921 agcgtttcaa catctggaaa tactgattct tcaacctgag taagcaagtt attgaaaaaa
    13981 tcaagttttg ttaattggtt attctgttta aa
//
LOCUS       DAXWIQ010000042        13533 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000042 DAXWIQ010000000
VERSION     DAXWIQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13533
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.042"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(310..1755)
                     /gene="gltX"
                     /locus_tag="ACGEUI_001389"
     CDS             complement(310..1755)
                     /gene="gltX"
                     /locus_tag="ACGEUI_001389"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFF8166218.1"
                     /translation="MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIE
                     DTDRKRHVEDGERSQLENLKWLGMDWDESPETHENYRQSERLALYQQYIDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEFIGMSADEKAKYIAEREAAGIVPTVRLAV
                     NESGIYKWTDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     PEAVFNFIALLGWNPGGEEEIFSREQLIALFDENRLSKSPAAFDQKKMDWMSNEYLKH
                     ADFETVYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEA
                     EKEVMAGETVSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGRDKSIEHIKNML"
     gene            complement(1937..2647)
                     /locus_tag="ACGEUI_001390"
     CDS             complement(1937..2647)
                     /locus_tag="ACGEUI_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HFF8166219.1"
                     /translation="MVDNRMRFTIDQNMQFPLVEIDLEHGGSVYLQQGSMVYHTENVT
                     LNTKLNGKGSGLGKLVGAIGRSMVSGESMFITQAMSNGDGKLALAPNTPGQIVALELG
                     EKQYRLNDGAFLALDGSAQYKMERQNIGKALFGGQGGLFVMTTEGLGTLLANSFGSIK
                     KITLDGGTMTIDNAHVVAWSRELDYDIHLENGFMQSIGTGEGVVNTFRGHGEIYIQSL
                     NLEQFAGTLKRYLPTSSN"
     gene            complement(2778..3275)
                     /locus_tag="ACGEUI_001391"
     CDS             complement(2778..3275)
                     /locus_tag="ACGEUI_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFF8166220.1"
                     /translation="MSYFEHFMAANQAYVALHGTAHLPLKPKTKVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFTNEGF
                     AKHIHEHLGVDVSGQDFLPFQDVEDSVREDMAKIRASSLISDDVVINGAVYDVDTGKM
                     TQVME"
     gene            complement(3464..4825)
                     /gene="radA"
                     /locus_tag="ACGEUI_001392"
     CDS             complement(3464..4825)
                     /gene="radA"
                     /locus_tag="ACGEUI_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFF8166221.1"
                     /translation="MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKN
                     ARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRVLGGGVVPGSLILIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
                     NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNARRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGE
                     IGLTGEIRRVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLNA
                     VFS"
     gene            complement(4906..5352)
                     /locus_tag="ACGEUI_001393"
     CDS             complement(4906..5352)
                     /locus_tag="ACGEUI_001393"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFF8166222.1"
                     /translation="MTKIRGFELVSSFTNPDLLPKRETTHAAGYDLSVAEAVTIAPGE
                     IKLVPTGVKAYMQDGEVLYLYDRSSNPRKKGIILINSVGVIDADYYGNEANEGHIFAQ
                     MQNITDHPVTLAVGERIVQGVFMPFLIADGDQARGERTGGFGSTGQ"
     gene            complement(5462..6229)
                     /locus_tag="ACGEUI_001394"
     CDS             complement(5462..6229)
                     /locus_tag="ACGEUI_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFF8166223.1"
                     /translation="MIDLQEILANMNPNQKINYDRVMQQMAKVWEKESVRPSILMHVC
                     CAPCSTYTLEYLTQFADITVYFANSNIHPKDEYHRRAYVTQQFVSEFNAKTGNTVQFL
                     EADYVPNEYVRQVRGLEEEPEGGDRCRVCFDYRLDKTAQKAVELGFDYFASALTISPH
                     KNSQTINDVGIDVQKVYTTKYLPSDFKKNNGYRRSVEMCEEYDIYRQCYCGCVYAAKM
                     QGIDLVQVKKDAKAFMADKDLDNDFTHIRFSYRGDEM"
     gene            complement(6347..8131)
                     /locus_tag="ACGEUI_001395"
     CDS             complement(6347..8131)
                     /locus_tag="ACGEUI_001395"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8166224.1"
                     /translation="MKTARFFWFYFKRYRFSFTVIAVAVILATYLQVKAPVFLGESLT
                     ELGKIGQAYYVAKMSGQTHFSPDLSAFNAVMFKLLMTYFFTVLANLIYSFLLTRVVSH
                     STNRMRKGLFGKLERLTVAFFDRHKDGEILSRFTSDLDNIQNSLNQSLIQVVTNIALY
                     IGLVWMMFRQDSRLALLTIASTPVALIFLVINIRLARKYTNIQQQEVSALNAFMDETI
                     SGQKAIIVQGVQEDTMTAFLKHNERVRQATFKRRLFSGQLFPVMNGMSLINTAIVIFV
                     GSTIVLSDKSMPAAAALGLVVTFVQYSQQYYQPMMQIASSWGELQLAFTGAHRIQEMF
                     DETEEVRPQNAPAFTSLKEAVAINHVDFGYLPGQKVLSDVSIVAPKGKMIAVVGPTGS
                     GKTTIMNLINRFYDVDAGSITFDGRDIRDYDLDSLRQKVGIVLQESVLFSGTITDNIR
                     FGDQTISQDMVETAARATHIHDFIMSLPKGYNTYVSDDDNVFSTGQKQLISIARTLLT
                     DPEVLILDEATSNVDTVTESKIQRAMEAIVAGRTSFVIAHRLKTILNADHIIVLKDGK
                     VIEQGNHHELLHQKGFYAELYHNQFVFE"
     gene            complement(8134..9840)
                     /locus_tag="ACGEUI_001396"
     CDS             complement(8134..9840)
                     /locus_tag="ACGEUI_001396"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8166225.1"
                     /translation="MLKDAILRYKWYALGSFFMVIGVVASSLLQPHYLKSVLSAVIQN
                     NQEKIYEVGKPLLIIALLGLMSGAINTVLAAKIAQGVSADMREKTFRKIQDFSYANIE
                     AFNAGNLVVRLTNDINQIQSLVMMMFQILFRLPILFIGAFIMAVQTFPQLWWVIVVMV
                     ILIALIMGLVMRQMGPRFGKFQRLMDKINRIAKENLRGVRVVKSFVQEQQQYTKFKET
                     SNDLLALNLSIGYGFSLMQPALMLVSYLAVYVSINVVSTMVETDPTVIGNIASFMTYM
                     MQIMFSIIVVGSMGMQVSRAFVSMARIRQILSTEPAMTFENEKEETISGSIVFDDVSF
                     TYPNDDEPTLKHISFAIEPGQMVGIVGATGSGKSTLAQLIPRLFDPQDGQILLGGKPI
                     KTLSQTTLRQSVSIVLQKAILFSGTIADNLRQGSAKADIDAMQKAAQIAQAKEFIDRM
                     DSRYESQVEERGSNLSGGQKQRLSIARGVINHPKILILDDSTSALDAKSEKRVQEALS
                     HKLEGTTTVIVAQKISSVVKADKILVLDQGQLIGEGTHAELVANNAIYREIYETQKGR
                     EE"
     gene            complement(9833..10282)
                     /locus_tag="ACGEUI_001397"
     CDS             complement(9833..10282)
                     /locus_tag="ACGEUI_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFF8166226.1"
                     /translation="MSQVIGDLRELIHQIEQISDEIAKKYDVEHLAGPQGYVLVFLAK
                     HQNQEIFVKDIEKQLRISKSVASHLVKRMVKNGFINVMPSQVDKRYKQVVLAQVGRDK
                     LPLLRECRKDIEHYFLKEITKEELLTAKKVIEQLKQNMLTYKGDNDA"
     gene            10580..11596
                     /locus_tag="ACGEUI_001398"
     CDS             10580..11596
                     /locus_tag="ACGEUI_001398"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFF8166227.1"
                     /translation="MTKQKVAILGPGSWGTALSQVLNDNGHDVRLWGNIPDQIEEINT
                     KHTNRHYFKDIVLDKNITATLDLGQALSDVDAVLFVVPTKVTRLVARQVAAILDHKVV
                     VMHASKGLEPETHERLSTILEEEIPAHFRSEVVVVSGPSHAEETIVRDITLITAASKD
                     IEAAKYVQSLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAVI
                     TRGLAEITRLGVKLGADPLTYSGLSGVGDLIVTGTSVHSRNWRAGAALGRGEKLEDIE
                     RNMGMVIEGIATTKVAYEIAQDLGVYMPITTAIYKSIYEGADIKESILGMMSNEFRSE
                     NEWH"
     gene            11629..12528
                     /gene="galU"
                     /locus_tag="ACGEUI_001399"
     CDS             11629..12528
                     /gene="galU"
                     /locus_tag="ACGEUI_001399"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFF8166228.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILIVTGKSKRSIEDHFDSNFELEYNLQAKGKIELLKLVDETTSINLHFIR
                     QSHPRGLGDAVLQAKTFVGNEPFVVMLGDDLMDITNPNVKPLTKQLIDDYEETHAATI
                     AVMRVPHEDVSNYGIIAPQAKAVKGLYSVDTFVEKPQPQDAPSDLAIIGRYLLTPEIF
                     SILEKQEPGAGNEVQLTDAIDTLNKTQRVFAREFKGKRYDVGDKFGFMKTSLDYALKH
                     PQVKDDLKAYIIQLGKALEKTKP"
     gene            complement(12627..13298)
                     /locus_tag="ACGEUI_001400"
     CDS             complement(12627..13298)
                     /locus_tag="ACGEUI_001400"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFF8166229.1"
                     /translation="MTQLLKRYPITIFLLGLTGLIFIAMQVVYGHLATGAQAIYQVGG
                     MFGLLVKAMPDQLWRLVTPIFIHIGFGHFFVNGLTLYFVGQIVEDLWGSRRFLLLYVL
                     SGVMGNAFTFWLTPETVAAGASTSLFGLFAAIVVLSFLGKNQALKDLGKSYQTLIVVN
                     LLMNLFMPNVSMAGHIGGVVGGALLSIVFPTKMRVITVKKTKRMLALVSYGIILVGVL
                     VLGFL"
BASE COUNT         4130 a         2951 c         2450 g         4002 t
ORIGIN      
        1 gagtactaaa ctagtctctt tcagtatata ccaaaaaaat attgtacact aattgattca
       61 taaaaaccat ttctttaaat cacactaatc aaaacctcac cgtttgctgt cattatcacg
      121 taagcatctc accacctctc tttcaaatcc aatctccaaa atagaaataa actatactat
      181 tttttacttt accaaggtag caagctcaga taacgataaa aaagactgaa gaacttaatc
      241 ttcaatcttt cgttgtcgtg actttttaac tccttaaagc gtttaaaaat aaaggtgaac
      301 gatacagcct tatagcatgt ttttgatgtg ctcaatggat ttatcacgtc ccaataaata
      361 aatcgtattt ggtaattctg ggccatgcat ttcaccagat actgcaatac gaataggcat
      421 aaagaggttt ttccctttga ttcctgtttc tttttgaaca gccttaattt gtgggaaaat
      481 attttctggt ttgaagtctt catctgacat agcttccaac ttagccttga aagcttgcaa
      541 gactgtcgaa actgtttcac ctgccatcac ttctttttcg gcttctgtta attcagggaa
      601 atcagaaaag aaaaggtcag tcaatggaat aatctcatct gcagatttta attgaggttt
      661 gtataattct actagcttct cagctttttc tgtcaaacgg cctgcctctt caaggaatgg
      721 tttgcagagg gcataaactg tttcaaaatc cgcatgttta agatactcat tgctcatcca
      781 atccattttc ttctggtcaa aggcagctgg cgacttgcta aggcgatttt catcaaaaag
      841 ggcaatcagt tgctcacgag agaagatttc ctcttctccg ccaggattcc agccaagaag
      901 ggcgatgaag ttaaagacag cttcaggcat ataacctttt ttgcggtaat cttcaatgaa
      961 ttggagggta ttggtgtcgc gttttgataa ttttttaccc gtttcagaat tgatgataag
     1021 agtcatatga ccaaactctg gcgcttccca gccaagagct tcatagacca tcaattgttt
     1081 tggcgtattg gcaatatggt catcgccacg gataacgtga gaaatttgca tatcatgatc
     1141 atcaacaaca acggcaaagt tataagttgg atagccatct ttcttttgaa taacccagtc
     1201 accaccgatg ttaccacctt caaactcgat atcgccttta accatgtcag tccatttgta
     1261 aataccggac tcattaacag caagacggac tgttggaacg atacctgctg cttcacgctc
     1321 agcaatgtac ttagcttttt cgtcagctga cataccaata aattcattga tatagcgagg
     1381 cgtttcgcca gctgcttctt ggcgttcacg ctctgctgcc aattcttcct cagtcacgta
     1441 agacttgtag gcttttcctt cagctaacaa ttgatcaata tattgttgat aaagtgctag
     1501 acgttcagac tggcggtaat tttcatgcgt ttctgggctt tcatcccaat ccattcctaa
     1561 ccattttagg ttttcaagtt gagaacgttc accatcttca acatgacgtt tacggtcagt
     1621 atcttcaata cgaatgataa aagtacctcc gtgacgacgg gcataaaggt aattaaatag
     1681 ggctgtgcgg gcattcccga tatgtaaaag ccctgttgga cttggtgcat aacgcacacg
     1741 aattggttta gacatgcttc ttctcctgtt acatatatca ctcagaaacc tgtggcacga
     1801 aaaacaagca acaaatgatg atttgagctt gccaagacct tttcagactt cttctaaaat
     1861 ctttcttatt atagcatatt ttacttgctt aggcgaaaaa aatcagccct aaaaagctga
     1921 ttttatgtta ttttgactaa tttgaactag ttggtagata acgtttaaga gtcccagcaa
     1981 attgctcaag gttaagactt tggatataaa tctcaccatg acctctaaag gtattaacaa
     2041 ctccttcacc tgtaccaatt gactgcataa agccattttc taaatgaata tcataatcaa
     2101 gttctctgct ccatgctaca acatgggcat tatcaatagt cattgtgcca ccatcaagtg
     2161 tgattttctt gatagatcca aacgagttag caagtaaggt accaaggcct tcagttgtca
     2221 tgacaaaaag tccgccttga ccaccaaaaa gtgcttttcc aatattttga cgttccattt
     2281 tgtactgtgc tgaaccatct agggctagaa aggcaccatc atttagacga tattgttttt
     2341 cacctagttc taaagcaaca atctgtcctg gtgtattggg tgctaatgct aatttaccat
     2401 ctccatttga catagcttgt gtaatgaaca ttgattcacc agaaaccatg gaacggccga
     2461 tggctccaac taattttcct aaaccagatc ccttaccatt caatttggta ttcaaggtta
     2521 cattctcagt atgataaacc atacttcctt gttgtaagta aactgatccc ccatgttcca
     2581 gatcaatctc aactagtgga aattgcatgt tttgatcaat cgtaaatctc attctattgt
     2641 cgaccatctt actctccttg tttgtatgaa taacttgata caattattat aacgcttccc
     2701 tatttctttg tctaatattt tcaataattt ttaaaaataa aaaagagctg aattcagctc
     2761 tacaacttgg ctactaacta ttccattact tgtgtcatct ttccagtatc cacatcgtag
     2821 acggcaccgt taataacgac atcatcagag ataagagagg aggctcttat cttagccata
     2881 tcctcgcgga cactgtcttc cacatcttgg aagggaagga aatcttgccc tgaaacatcc
     2941 acacctaagt gctcatgaat atgcttggca aagccttcat tagtaaaagt ctgcgcgcca
     3001 caatctgtat ggtggagaac cacaatctct ctcgttccca tttgctgctg agaaataacc
     3061 agcgaccgaa tcatgtcttc ggtgacacgt ccgccagcat tccttaaaat atgagcatct
     3121 cctagagcca gccccagagc ttgggccaca tgtagccgtg aatccataca agtcacaatc
     3181 gctactttag tcttgggttt taagggcaaa tgagctgtcc catgcaaggc cacataagcc
     3241 tgattggcag ccataaaatg ttcaaaataa gacatcaata aactcctttt gagaaacagc
     3301 agagctattt ttgctgttag tttgtcttat cttaacatct tttatggaaa atagctggat
     3361 taacctctta gacggtcggt atcgttcgaa aagttaaaaa aacaaatgat tgggatactt
     3421 tcttgacaaa tttcctcctc atttgctctt agtttctgat cctctagcta aaaactgcat
     3481 ttaaaacctg accaaccgta gtcactccga caacctcaat accttgaggg atatcaatgc
     3541 cttgcaaggc atttttagga gcatagacct tggtaaagcc aagctttgct gcttcattaa
     3601 tccgttgctc aatacgggtc actcgccgaa tctcacctgt taacccgatc tcgcctaaga
     3661 aagcttcttg agggctggtt ggtttttcct tataactgga tgcgatagcc acagcgactg
     3721 ccaaatcaat ggcaggttcg tctagtttga caccaccagc cgacttgagg taggcatctt
     3781 gattttgtaa gagtaaacca cagcgctttt ctaaaactgc cattattagg cttacgcgat
     3841 tgaaatcaag tcccgtagtg gttcgtctgg cattgccaaa aacggttggt gtcactaaag
     3901 attgcacctc tgctaagata ggacgacttc cttccatcgt tacaacgaca gccgatcctg
     3961 ttgccccatc taagcgttcc tctaaaaaga cttgactagg atttaagacc tctaccaagc
     4021 cgccagactg catttcaaaa atgccaatct cgttagtgga gccaaagcgg tttttcacag
     4081 cccgcagaat gcgaaaggtg tgatgccttt ccccttcaaa gtagagaacg gtatccacca
     4141 tatgttctaa catacgaggc ccagccaagg ttccttcctt ggtgacatga cccacaataa
     4201 aggttgcaat gttgttcgtc tttgcaagct gcatcaattc tgcagtgact tctctcacct
     4261 gactgactga tccttgaaca cctgtaatat cagggctcat gatggtctga atggagtcaa
     4321 taattaaaaa atctggttta atattttcaa tttctgtgcg aatagcttgc atattggttt
     4381 cggcatagag gtaaaattca ttgtcaatat cacccagacg ctcactccgc agtttaatct
     4441 gctctgctga ttcttcccca gacacataga gaaccgttcc cttattagcg agctgcgtgg
     4501 atacctgcaa gagtaaggtg gattttccaa tacctggatc accaccaatt aaaatcaagc
     4561 ttccaggaac cacaccccct ccaagaactc tattaaattc agacatgtca gtctgtgttc
     4621 tgtgataact aatattatcc acatctttta gtttaaccgg tctggacttt tctccagcta
     4681 gactaacacg ggcatttttg acttctttga cctctacttc ttccacaaaa gaagaccagg
     4741 ccgagcaatt tggacagcgc cctaaatact ttggcgactg gtagccacac tcctgacaga
     4801 taaaggttgc ttttttcttt gccatagtaa tctccctccc ctatacaaga caaacccact
     4861 cttaaagaat aggtctgctg tcataagcat ttttgttaaa tcgctttact gccctgtcga
     4921 accaaaacca cctgttcgtt cacctctggc ttggtcacca tcagcaatta aaaaaggcat
     4981 aaaaacaccc tgaacgatcc tctcacccac agctaatgtc acagggtgat ctgtaatgtt
     5041 ttgcatttgt gcaaaaatat gtccttcatt agcctcatta ccataataat ctgcatcaat
     5101 cacacctact gaattaatca ggataatccc tttttttcgt ggatttgacg aacgatcata
     5161 gaggtaaagc acctcgccat cttgcatata ggccttaacc cctgttggta ccaacttaat
     5221 ctcccctggg gcaatggtta cagcctcagc aacagacagg tcataaccag ctgcatgagt
     5281 cgtctcacgt tttggtagta aatcaggatt agtaaagctt gaaacaagct cgaaaccacg
     5341 aatttttgtc attatgatag gtatatcctt tcaagtcaat catatcctct tattatagac
     5401 tattcgtaaa agaatagcaa aaaagcggtc aactttaaac ggaccacttt taatcatgcc
     5461 atcacatctc atccccacga taactaaagc ggatgtgggt gaaatcatta tccaaatcct
     5521 tgtctgccat aaaagctttg gcatcttttt tcacttgaac caagtcaata ccttgcattt
     5581 tggcagcata gacacagcca caatagcatt ggcggtaaat atcatactcc tcacacattt
     5641 caacggatct gcgataaccg ttgtttttct tgaaatcact tggcaggtat ttggtggtgt
     5701 aaaccttttg cacatcgatg cccacatcat taatggtttg cgaattttta tgaggactga
     5761 tggttagggc actggcaaag tagtcaaaac caagttccac tgctttttga gcggtcttgt
     5821 ctagtcggta atcaaaacag acacggcaac ggtccccacc ttctggttcc tcttctaagc
     5881 ctcgcacttg tcgaacgtac tcattgggga catagtccgc ttcgagaaac tgaacagtat
     5941 tgcctgtttt agcgttgaac tcggagacaa actgttgtgt cacataagct ctgcgatggt
     6001 attcgtcctt agggtgaata ttagaattgg caaagtaaac cgtaatgtca gcaaactgcg
     6061 tcaggtattc tagggtgtaa gtggagcatg gggcgcagca gacatgcatt aaaatactgg
     6121 ggcgcacgga ttccttctcc caaaccttgg ccatctgttg catgaccctg tcataattta
     6181 tcttttgatt gggattcatg ttagcgagaa tctcttgtaa atcaatcatt gtggctactt
     6241 tctaattgtt ttttctcttg cttgctatta taccatatca gaaaagaaag cttgactgct
     6301 ttattgttca ccaaaaaact ccgctaagac gcggagctga tgccatctat tcaaagacaa
     6361 attgattgtg atacaattcg gcataaaagc ctttttgatg caatagctca tgatgatttc
     6421 cttgctcaat gaccttgcca tctttcaaca caataatgtg atcggcattt aaaatggttt
     6481 tgaggcggtg agcaatgaca aagctagttc gacctgccac gatagcttcc atggcccgtt
     6541 gaattttact ttcggtaacc gtatcaacat ttgaagtggc ctcatccaaa atcaacactt
     6601 cagggtcagt cagtagcgtc ctagcaatag aaatcaactg cttttgacct gttgaaaaga
     6661 cattgtcatc atctgagaca taggtattgt acccttttgg taaggacatg ataaagtcat
     6721 gaatatgggt cgcacgcgca gcagtttcaa ccatgtcttg actaatggtc tgatcaccaa
     6781 aacgaatatt atccgtaatg gttcctgaaa aaagaactga ctcttgcaac acaatcccta
     6841 ccttttgacg aagactatcc aaatcgtagt cacgaatatc acggccatca aaggtaatcg
     6901 aacctgcatc cacatcgtag aaacggttaa tcaagttcat aatagtggtc tttccagaac
     6961 ctgtcggtcc aaccacggca atcattttgc ccttgggtgc aacgattgac acatctgata
     7021 aaactttttg cccaggaaga tacccaaaat cgacgtggtt aatcgccact gcttctttta
     7081 agctggtgaa cgctggtgca ttttgtggac gaacttcttc ggtttcatca aacatttctt
     7141 gaatacggtg agcaccggta aaggccagct gcaattctcc ccaactagac gcgatttgca
     7201 tcatgggttg gtaatattgc tgggaatatt gtacaaaagt aaccactaaa ccaagcgctg
     7261 ccgctgctgg catagatttg tcactgagga caattgttga accgacaaaa atcacgatag
     7321 ccgtgttaat aaggctcatt ccattcatga ctggaaataa ttgtcctgag aacagacggc
     7381 gtttgaaggt ggcttgtcga accctttcat tatgctttaa aaaggctgtc atcgtatctt
     7441 cttggacacc ttgtacaata atagcctttt gtcctgaaat ggtttcatcc ataaaagcat
     7501 ttaaagcact gacttcttgc tgttggatat tggtgtattt tcttgccaaa cggatgttaa
     7561 tcactaaaaa aatgagagca actggggttg atgcgatggt taacaaagct aaacggctat
     7621 cttgcctaaa catcatccag accaggccga tgtaaagggc aatattagtc accacttgaa
     7681 tcaaggattg gttcagcgag ttttgaatat tatccaaatc actcgtgaaa cgagaaagaa
     7741 tctccccatc tttatggcgg tcaaaaaagg cgacggttaa acgttctaat ttaccaaata
     7801 agcccttgcg catgcggtta gtcgaatgtg agacaacacg tgtaagtaag aaactatata
     7861 ttagattagc taaaacagta aagaaataag tcatcaaaag cttaaacatc acggcattaa
     7921 aagctgataa atcagggcta aaatgtgtct ggccactcat cttagcaacg taataagcct
     7981 gaccgatttt tcccaactca gtcaaggact ctcctaagaa gacaggagct tttacttgta
     8041 aataagttgc taagataacg gcaacagcaa tgacagtaaa tgagaaacga tagcgtttaa
     8101 aataaaacca gaaaaaacgt gctgttttca tcactactcc tcccttcctt tttgtgtctc
     8161 ataaatttca cgataaatcg cattattagc caccaactct gcgtgtgttc cttctccaat
     8221 taactgtcct tggtcaagga ctaagatctt atctgcctta actaccgatg aaattttttg
     8281 agcaacaata actgtagttg taccttctaa tttatgactt aaggcttctt gaacgcgttt
     8341 ttcagatttt gcatcaagag ctgaagtgga atcgtctaat ataagaattt tgggatgatt
     8401 aatgacacca cgtgcaatag acagacgttg cttttgtccg ccagacaagt tactacctcg
     8461 ctcttcaact tggctctcat aacggctatc catacgatca ataaattctt ttgcttgcgc
     8521 gatttgagcg gctttttgca tagcatcaat atcagctttt gctgagcctt ggcgaaggtt
     8581 atcagctatc gttcctgaaa agagaatagc cttttgcaac acgatggaaa ccgattggcg
     8641 cagggttgtt tgacttaggg ttttaatcgg ctttcctcct aacaaaattt ggccatcttg
     8701 aggatcaaat aagcgaggga tgagctgtgc aagcgttgat ttcccagatc ctgttgcacc
     8761 aacgatgccg accatttgcc caggttcaat agcaaaagaa atgtgcttca aggttggctc
     8821 atcatcatta ggatatgtaa aactaacatc gtcaaataca atgcttcctg agatggtttc
     8881 ttccttttca ttttcaaatg tcatggcagg ttctgtagac aatatttgcc taattctagc
     8941 catagaaaca aaagcacgac taacttgcat ccccatggaa ccaaccacaa taattgaaaa
     9001 cataatttgc atcatataag tcatgaaaga ggcaatattc ccaatcactg ttggatctgt
     9061 ttcgaccatg gtcgaaacaa catttataga aacataaact gccaaataag acaccaacat
     9121 taaagccggc tgcattagtg aaaaaccata accaatagat aaatttaacg ccaaaagatc
     9181 attggacgtt tctttaaatt tagtgtattg ttgttgttct tgtacaaatg atttaaccac
     9241 gcgaacgcca cggagatttt ccttagcgat gcgattaatc ttatccatta accgttgaaa
     9301 tttaccaaaa cgaggaccca tttgacgcat caccaaaccc atgatgaggg caattaagat
     9361 aaccattaca acaatgaccc accataattg agggaatgtc tgaactgcca taataaacgc
     9421 gccaataaaa aggatcggca aacgaaagag aatttgaaac atcatcatga ctaaactttg
     9481 gatttgattg atatcattag ttagcctaac cacaagatta ccagcattaa aagcttcaat
     9541 attggcatat gaaaagtctt ggatttttcg aaaggtcttc tccctcatat cagcagatac
     9601 accttgagct attttagcag ctaaaaccgt attaatcgct ccagacatta accctaaaag
     9661 ggcaataata agtaatggtt tcccaacctc ataaattttc tcttgattat tttgaataac
     9721 agcgcttagc acagatttaa gataatgtgg ttgcaagaga ctacttgcaa caaccccaat
     9781 aaccataaaa aatgagccta aagcatacca tttataacgt aagattgcgt ctttaagcat
     9841 cgttgtctcc tttataagtt agcatatttt gcttgagctg ttcaattact tttttcgctg
     9901 tcagcaactc ttcttttgta atttctttta aaaaatagtg ctcgatatcc ttacgacact
     9961 cccgcaacaa aggcaattta tctctaccaa cctgcgctaa cactacttgc ttataacgct
    10021 tatccacttg ggaaggcatc acattgataa acccattttt gaccatacgt ttcactaaat
    10081 gactagcaac tgactttgag atacgaagtt gtttttcaat atctttgaca aatatttctt
    10141 gattttggtg tttagctaaa aaaacaagaa cataaccttg aggacctgct agatgctcta
    10201 catcatattt ttttgcaatc tcatcactaa tttgttcgat ttgatgtatc aattcacgta
    10261 aatcacctat cacttgtgac atctcttccc tccttatttt ctttaatagt ttctatgata
    10321 agtattttag ttctctagat aactattgtc aattaaaata tctttatttt taaaaataag
    10381 tttccgaact taaataacta aaaagaacag ggcaaagcca tgacaacagc tcacctgatg
    10441 aagcttatta aacaacatta ttaatatagc tagctctctt gggaacgcct tattagccaa
    10501 gaccatatac cttttctaat agagctaatc aaggtgttat gttataatag agtgactagc
    10561 tcttgaagga gaatttagaa tgacaaaaca aaaagtagct attttaggac ctggttcttg
    10621 gggaactgcc ctttctcagg ttcttaatga caatggccat gacgtccgtt tatggggaaa
    10681 tattcctgat caaattgaag agataaatac caagcatacc aatagacact acttcaaaga
    10741 cattgtgtta gacaaaaaca ttacggcaac tcttgattta ggtcaagccc tatcagatgt
    10801 tgatgcggtg ctttttgttg tgccaactaa agtaacacga ttagttgcta gacaagttgc
    10861 cgccatttta gatcataaag tagtggtcat gcatgcctca aaaggtctag aaccagagac
    10921 ccacgaacgt ttatcaacta tcctagaaga agaaattcca gctcattttc gtagtgaagt
    10981 ggtcgttgta tcaggtccta gtcatgctga agaaactatc gttcgcgata ttactctcat
    11041 cacggcagcc tcaaaagata ttgaagctgc aaagtatgtt cagtccctct ttagtaacca
    11101 ctacttccga ctttatacca ataccgacgt catcggagtt gaaacagctg gtgccctcaa
    11161 aaacattatt gctgttggag caggagctct ccatggactt gggtatggag ataatgccaa
    11221 agcagcggta attactcgtg ggttagctga aatcacccgt ctaggcgtca agcttggtgc
    11281 tgacccgtta acctacagtg ggttatctgg tgttggtgat ctaattgtaa caggtacttc
    11341 tgtccattca cgtaactggc gggctggagc tgcccttgga cgtggtgaaa aattagaaga
    11401 tattgaacgc aacatgggta tggttatcga aggtatcgca acaactaagg tagcctatga
    11461 aattgcccaa gacctcggag tttacatgcc aattacaaca gctatttata agtccatcta
    11521 cgagggcgct gatatcaaag aaagcatcct cggcatgatg tcaaatgaat ttcgttctga
    11581 aaatgaatgg cattaagcca tgcgtcacta aaataagagg aggtgctgat gaccaaagtc
    11641 agaaaagcta ttattcccgc agcaggttta ggcacacgtt ttttacctgc aaccaaggcc
    11701 cttgccaagg agatgctacc tattgttgat aaaccaacta ttcaatttat cgttgaagaa
    11761 gctctaaaat caggtatcga agaaattctt atcgttactg gaaaatccaa acgctccata
    11821 gaagatcatt ttgattctaa ttttgaatta gaatataatt tacaggctaa aggaaaaatc
    11881 gaacttctaa agttagtaga cgagaccact tctattaatc ttcactttat tcgacagagt
    11941 catccaagag gattaggaga cgcagttctt caagcaaaga cctttgttgg caatgaacct
    12001 tttgttgtca tgctaggtga tgatctgatg gatattacaa atccaaatgt aaaaccatta
    12061 accaaacaat tgatcgatga ctatgaagaa actcatgctg caactattgc cgtcatgagg
    12121 gttcctcacg aggatgtctc taactacggt atcattgctc ctcaagcaaa agcagtaaaa
    12181 ggactctata gtgtggatac ttttgttgaa aaaccacaac ctcaagatgc acctagtgat
    12241 ttggcaatta ttggacgtta cttgctcaca ccagaaattt ttagtatttt agaaaaacaa
    12301 gaacctggtg caggcaatga agttcaactg actgatgcta ttgataccct caataagaca
    12361 cagcgtgtct ttgctcgtga gtttaaaggg aaacgctatg atgttggcga taaatttggg
    12421 tttatgaaaa cttctttgga ttatgccctg aaacaccctc aagtcaaaga tgacttgaaa
    12481 gcctacatca tccaactggg caaagcacta gagaagacaa aaccataaaa ataaggaggg
    12541 aaaatccctc cttattttta tggtttttaa gtcatttcat cctactagcc atctgagaag
    12601 taacagtgct aaccactcac ttatttttat aaaaacccaa gcactaacac accaaccaaa
    12661 ataataccat agctcactaa agccaacatt cgcttggttt tcttcactgt tatcactctc
    12721 atcttagttg gaaaaactat actcagtaac gcaccaccca cgacaccgcc aatatggcct
    12781 gccatactca cgtttggcat aaaaagattc atcagtaaat tgactacaat taacgtctgg
    12841 tatgacttgc ccaaatcttt caacgcttgg ttcttaccca aaaaactcaa tactacaata
    12901 gcagcaaaaa gtccaaataa cgacgtcgat gctcctgcag ctactgtttc aggagtcaac
    12961 caaaaagtaa aggcattgcc cataacacct gataaaacat aaagtaataa aaaacggcgt
    13021 gagccccaga ggtcttcgac gatttgacca acaaaatata gtgttaatcc attgacaaaa
    13081 aaatgaccaa agccgatatg aataaatatc ggagtcacca atcgccacaa ctggtctggc
    13141 attgctttta ctagtaatcc aaacatacct ccaacttgat aaatagcttg agcaccagtt
    13201 gctaaatgtc catataccac ttgcattgct ataaaaatta agcctgttaa ccctaaaagg
    13261 aaaatagtaa taggatatcg ttttaataac tgtgtcatct aagataaacc tcctaaacag
    13321 caatatcata aatcgtttga tttggcaatc gtattggccc gctgataagc ttgattgcta
    13381 acaacatcca ctaataaagc ttgatcattt tgacgtttta agtctacatc catagttcgt
    13441 aaagactaga agagttcttt gagaatctct tttttcatca tcttcttatc ataccataaa
    13501 ttaggaaaaa ggttcaaggc tgacaagaaa cta
//
LOCUS       DAXWIQ010000043        13325 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000043 DAXWIQ010000000
VERSION     DAXWIQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13325
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.043"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            2..2176
                     /locus_tag="ACGEUI_001401"
     CDS             2..2176
                     /locus_tag="ACGEUI_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFF8166230.1"
                     /translation="MTHKIKVLLLAIMSIFLTCNIASAETIAIVSDTAYAPFEFKDSD
                     QIYKGIDVDIINEVAKRQSWDFSMSFPGFDAAVNAVQSGQASALMAGTTITNARKKVF
                     HFSEPYYDTKIVIATRKANAIKKYSDLKGKTVGVKNGTAAQAFLNNYKKKYDYTVKTF
                     DTGDLMYNSLSAGSIAAVMDDEAVIQYAISQNQDIAINMKGEPIGSFGFAVKKGSGYD
                     YLVNDFNTALKAMKADGTYQAIMTKWLGTDDKATTSQATGNPSAKATPTKDSYKIVSD
                     SSFAPFEFQNGKGKYVGIDIELIKAIAKQQGFKIEIANPGFDAALNAVQSSQADGVIA
                     GATITDARKAIFDFSDPYYTSNIILAVKAGKNIKNYEDLDRKTVGAKNGTSSYSWLKE
                     NAPKYGYNVKAFDDGSSMYDSLNSGSVDAIMDDEAVLKYAISQGRRFETPLEGISTGE
                     VGFAVKKGTNPELIEMFNNGLAALKKSGQYDDIIDKYLDSKKAATPSEKGADESTISG
                     LLSNNYKQLLAGLGTTLSLTLISFAIAIIIGIIFGMMAVSPTKSLRLISTVFVDVVRG
                     IPLMIVAAFIFWGVPNLIESMTGHQSPINDFLAATIALSLNGGAYIAEIVRGGIEAVP
                     AGQMEASRSLGLSYGTTMRKVILPQAVKLMLPNFINQFVISLKDTTIVSAIGLVELFQ
                     TGKIIIARNYQSFRMYAILAIIYLIMITLLTRLAKRLEKRLN"
     gene            2176..2916
                     /locus_tag="ACGEUI_001402"
     CDS             2176..2916
                     /locus_tag="ACGEUI_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8166231.1"
                     /translation="MTELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLETITSGKVMVDGFELSDPKTNIDKARENIGMVFQHFNLFPHMTVLENI
                     IFAPVELGKESKEVAKKHGMALLEKVGLSDKADAFPGSLSGGQKQRVAIARSLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGQF
                     LEDGTPEEIFDHPKHPRLIEFLDKVLNV"
     gene            complement(3064..3216)
                     /locus_tag="ACGEUI_001403"
     CDS             complement(3064..3216)
                     /locus_tag="ACGEUI_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166232.1"
                     /translation="MTEKNGATFLMAVGFFLIAISPMVGNKYLHIVTGLALTGLGYYL
                     TKHKRP"
     gene            complement(3213..4592)
                     /locus_tag="ACGEUI_001404"
     CDS             complement(3213..4592)
                     /locus_tag="ACGEUI_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8166233.1"
                     /translation="MSHFSDKFMEISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLL
                     NVFLRDIPNNMGWTGFAKAMQPVIDINGYVYFGTIGIMALFFAFAFGYNLAVMHKINP
                     LAGGLISFGSFIATLPQTLTISTPLENASANLISNLKEMGLSVVTAGGASSIETSQWG
                     AIALKYVGATGLFTALIIGFLSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTV
                     AIYASAIFAYLIFALTGSSLSDVISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLH
                     GHNVLAPVMDGIYMVALTENTAAYNTAHSAANLPYLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEHKTIAKLSAPMGVFNINEPITFGMPIVLNPTFVIPWLIVPPICASIA
                     YFATAIGLIPPVFLSVPWITPVGLYAYLATGGNIMAGLVSLFNLFVAFLIWAPFVILA
                     NKEKASDLA"
     gene            complement(4770..5219)
                     /locus_tag="ACGEUI_001405"
     CDS             complement(4770..5219)
                     /locus_tag="ACGEUI_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFF8166234.1"
                     /translation="MIISAEGDVSIHHCFEAMLISLQEDYRQNTLRQLSRDDIKAGIN
                     YVKHYGKYKERTVRVLITALEAPQLYTACFSSNHGIKELAYQLEALGENRTRITYSYD
                     YHPLDIFQKANQFIVGKLFKKSLERQSQAQLAALIQYAKQLQQPSSL"
     gene            complement(5216..5551)
                     /locus_tag="ACGEUI_001406"
     CDS             complement(5216..5551)
                     /locus_tag="ACGEUI_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8166235.1"
                     /translation="MTEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQ
                     EATNSLNIAHKSQTHLLSQEAAGDAVELSLLMVHGQDHMMTALAFIDLAKELVTVYDK
                     MSAIQRGES"
     gene            complement(5554..5865)
                     /locus_tag="ACGEUI_001407"
     CDS             complement(5554..5865)
                     /locus_tag="ACGEUI_001407"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8166236.1"
                     /translation="MKSIMLVCNAGMSTSMLVTKMQKAAQARDLEVSIWAVPVSEADN
                     EMAANSIDVLLLGPQVKFLLKDFKDKFEPDIKVDAINMADYGLMNGEKVLETALAMME
                     E"
     gene            complement(5888..7882)
                     /locus_tag="ACGEUI_001408"
     CDS             complement(5888..7882)
                     /locus_tag="ACGEUI_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8166237.1"
                     /translation="MLSKKELAIITFLIHHKEQFVSSATLAEVIGMSDRTVRKYLKEL
                     ISSLPEHGAHIISKQGRGYCLEIDHSMAFDIFWQESVTSKKRLADVTQVEESVDREDY
                     VLHKFFFEDAVQDFEELCQELYISRTTLKHVLAGIKERIIPYQLELEITHQHIQIRGK
                     EEDIRHFIMDYFFVTSFDNTLSTMVGNTFLEGINFAEIMIIVLDECRDAKLKLSDFVM
                     NNLVLHIALMVQRIRSGCPLELFSIPIAIRQSDEYQVALRILYRVEEVMGIRFPKEEA
                     NYIALHLKVKHSVGKHWQDDNTDEKLQDHLKACIAKISQLTGMTLETDTNLFQGLLAH
                     MMPLTIRLENHIQLTNPLTEEIKSQYPEIFTLTKQTFSDLLVCQKNDVSDDEWAYISL
                     HLMAAIERYSNRHKLRVLVVCATGYGSAMMLKNRLEKEFEGRLRIVDVISYYEITEER
                     LKTVDLIISSISLANLMFLTPVITVSVFLSNQDIETIRQFIGEQEGIKKEVSLPSQMS
                     LAKAEQLLTGVFSPNRFLYLDEKISKEDLLLRMIACLDEAGTETFVEDFYHQMVLREN
                     YSPVIYGEVLAFPHPANPMTYSEQVVVVICREPLEWDKAHQAVHFVFLLSPSKGHNHR
                     LKYVSPGLASFVNQVELQQALLEEPNYSKFMTVFTPLIHD"
     gene            complement(7988..9082)
                     /locus_tag="ACGEUI_001409"
     CDS             complement(7988..9082)
                     /locus_tag="ACGEUI_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFF8166238.1"
                     /translation="MVDLGFSLYPERYDVTKSKAYIDLCHSYGAKRLFMSLLQLAPAD
                     HQMFHCYAELIAYANQLGIRVIADVSPSFISQAGWSDQLIERAHAFGLAGLRLDEALP
                     LAEIVTLTRNPFGLKIELNMSTDKQLLMSLLATDAERSNIIGCHNFYPHEFTGLSWQH
                     FKDMSRFYHEHDIETAAFITAQSASEGPWLLAEGLPTVEDHRHLPIGLQVELMKAIGT
                     IDNILISNQFISEEELAACTQALARPVTTIKVRPIIDLTEVEEQIIGYPHCYRGDVSD
                     YVIRSTMPRLVYAQESIAPRDQSKEVKRGSIIIDNDRYHRYKGELQIALKNFTVSSKA
                     NVVAEVREDYLSLLDDLRPWQEFCLEIAPS"
     gene            complement(9091..10491)
                     /locus_tag="ACGEUI_001410"
     CDS             complement(9091..10491)
                     /locus_tag="ACGEUI_001410"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8166239.1"
                     /translation="MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFS
                     IESAKFHHQIGPEKTSTFYENYKGDIALLKETGHTIFRTSIQWSRLIPEGVGEVNPKA
                     VTFYREVFQDIIAQGIKLIVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFG
                     DLVNTWITFNEPIVPVECGYLGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHP
                     DHKISIVLNMTPAYPRSNAPEDVKAARIAELFQTKSFLDPSVLGVYPAELVSILEEAD
                     LLPQYSADELEIIKNNTVDFLGVNYYQPLRVQAPSKSQQEGDLLILDIYFEPYDMPGK
                     KVNPHRGWEIYEPGLYDIALNLKEHYGNIEWLVTENGMGVEGEEAFLADGQIQDDYRI
                     TFIEDHLIQLHKALGEGANCKGYLLWTFIDCWSWLNAYKNRYGLVALDLESQKRTIKK
                     SGYWFKALSESNGFDK"
     gene            10716..11411
                     /gene="pnuC"
                     /locus_tag="ACGEUI_001411"
     CDS             10716..11411
                     /gene="pnuC"
                     /locus_tag="ACGEUI_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFF8166240.1"
                     /translation="MAYLNYFTKTEWALWLSSVLAILISALLFGKQAPLALIASLIGV
                     TSLIFSAKANPIGQGLVIIFSIIYAYLSLRNSYYGELMTYLFMTLPMTIFSLFTWLNH
                     PFEGKKSQVTISRLTPTDRRCLFVFTILITLIFYSILASIQTAYLLVSTLSIATSFSA
                     VYLSYKRSPYFALAYGLNDLVLILLWMHAAQTDASQYAVVICFATFLINDSYTFYNWL
                     MLQRYQEKKVKKG"
     gene            complement(11416..11574)
                     /locus_tag="ACGEUI_001412"
     CDS             complement(11416..11574)
                     /locus_tag="ACGEUI_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166241.1"
                     /translation="MGDKPISFKDKDGNFVSAADVWNAEKLEELFNLLNPNRRLRLER
                     EKLKKDEA"
     gene            complement(11643..12956)
                     /gene="obgE"
                     /locus_tag="ACGEUI_001413"
     CDS             complement(11643..12956)
                     /gene="obgE"
                     /locus_tag="ACGEUI_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFF8166242.1"
                     /translation="MSMFLDTAKISVQAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFRVDEGLRTLMDFRYNRKFKAKSGEKGMTKGMHGRGAEDLIVFVPQGTTVRDAET
                     GKVITDLVEHGQEVVIAKGGRGGRGNIRFATPRNPAPEIAENGEPGEERQLELELKIL
                     ADVGLVGFPSVGKSTLLSVVSSAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYEDYVSINNELETYNLRLME
                     RPQIIVANKMDIPEAQENLKAFKKKLAAQYDEFDDLPMIFPISSLAHQGLENLLEATA
                     ELLAKTDEFLLYDESDLVDEEAYYGFAETEKDFEITRDDDATWVLSGEKLERLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDPVRIGKFEFEFVD"
     gene            complement(13013..13141)
                     /locus_tag="ACGEUI_001414"
     CDS             complement(13013..13141)
                     /locus_tag="ACGEUI_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFF8166243.1"
                     /translation="MAFGENGPRKKTTFEKVTMFVVILMVLVTVGGLIASALSVLM"
BASE COUNT         4041 a         2848 c         2425 g         4011 t
ORIGIN      
        1 tatgacgcac aaaataaaag tattgctgct tgcgataatg tctatttttt tgacatgcaa
       61 tattgcaagt gctgaaacta ttgctattgt ttcagataca gcttatgccc catttgaatt
      121 taaagactca gatcaaattt acaaaggaat tgacgttgat attattaatg aagtagccaa
      181 acgtcaatct tgggatttca gtatgagttt cccgggtttt gatgcagctg taaatgctgt
      241 tcaatctggt caagcgagtg ctctaatggc cggtacaacc attacgaatg ctcgtaagaa
      301 agtctttcat ttctcagagc catattacga taccaaaatt gtcattgcga cacgtaaagc
      361 caatgccatc aaaaaataca gtgacttaaa aggaaaaacg gtcggtgtta aaaatggaac
      421 agcggctcaa gcctttttga ataactataa aaaaaagtat gattatactg ttaaaacatt
      481 tgacacaggt gatcttatgt ataatagttt atctgctggt tctattgccg ctgttatgga
      541 tgatgaggcg gttatccaat acgcaatcag ccaaaaccaa gatattgcta ttaacatgaa
      601 aggagagccc attggaagct ttgggtttgc tgtcaaaaag ggaagcggat atgattatct
      661 agttaatgat ttcaatacag ctcttaaagc tatgaaagct gatggtacct accaagctat
      721 catgaccaag tggttaggca cagatgataa agctaccacc agtcaggcaa cgggaaatcc
      781 atctgccaaa gctacaccta caaaggacag ttataaaatt gtctctgatt cgtcttttgc
      841 accttttgaa tttcaaaatg gtaagggcaa atacgttggt attgacatag aattaatcaa
      901 agctattgct aaacaacaag gtttcaaaat tgaaatcgct aatccaggtt tcgatgctgc
      961 cttaaatgct gtgcaatcta gccaagcaga tggggtcatt gctggtgcaa ctattactga
     1021 cgctcgtaaa gctatctttg atttttctga tccttattat acttctaata tcattttagc
     1081 tgttaaagct ggaaaaaaca tcaagaacta tgaagactta gacagaaaaa cagtcggtgc
     1141 taaaaacggc acttcatctt actcttggtt aaaagaaaac gctcctaaat atggttataa
     1201 tgtcaaggca tttgatgatg gttctagcat gtatgatagc ttaaattcag gttctgtaga
     1261 tgctatcatg gatgatgagg cggttcttaa atacgctatc tctcaaggtc gtcgctttga
     1321 aacacctctt gagggcattt ctactggtga agttggtttt gctgtcaaga aaggaactaa
     1381 tccagaatta atcgaaatgt tcaacaatgg cttagctgct ctcaaaaaat ctggtcagta
     1441 tgatgacatt atagataaat accttgactc taagaaagct gcaactcctt ctgaaaaagg
     1501 tgctgatgag tctactattt caggcctatt atcaaataac tacaaacaac tattggcagg
     1561 acttggaacc acgctcagtt taacccttat ttcatttgct attgctataa ttatcgggat
     1621 catctttggg atgatggccg tgtcaccaac taaatcactt cgacttattt caacggtctt
     1681 tgtggacgtt gttcgaggga ttcctttgat gattgtggct gccttcattt tctggggagt
     1741 accaaacctt atcgagagta tgaccggcca ccagtcaccg attaatgatt tcttagctgc
     1801 tacaattgca ctgtcactta atggcggagc ctatattgct gaaattgttc gcggtggtat
     1861 cgaagctgtt ccagcagggc aaatggaagc tagtcgaagt cttggtttgt cttacggaac
     1921 cacgatgaga aaagtaattc tcccacaagc tgtgaaacta atgttaccta actttatcaa
     1981 tcagtttgtt atttcattga aggatacaac aatcgtctca gcaattggtt tagtggaact
     2041 cttccaaaca ggtaaaatca ttattgctag aaattaccag tcgttccgta tgtatgctat
     2101 tttagcaatt atttacctta tcatgattac actcttaaca agacttgcaa aacgtttaga
     2161 aaagaggctt aactaatgac agaattaaaa attgatgttc aagatttaca caaatcttat
     2221 ggtcaaaatg aagtcttaaa aggaattgat gccaaattct acgaaggcga tgtcgtttgt
     2281 atcattggtc cttctggttc tgggaaatca acctttctac gtaccctcaa cctcctagag
     2341 acgattacca gtggtaaggt catggtggac ggttttgaat tatcagatcc taagactaat
     2401 attgacaagg cacgtgaaaa tatcgggatg gtattccaac acttcaacct tttccctcac
     2461 atgactgttc ttgagaatat tatctttgct cctgttgaat taggtaaaga gtctaaagaa
     2521 gttgctaaaa agcatggcat ggcacttcta gaaaaagtcg gactttctga taaggccgac
     2581 gccttcccag gaagtttatc cggcgggcaa aaacagcgtg tcgccatcgc cagaagttta
     2641 gcaatgaacc cagatattat gctctttgat gaaccaactt ctgctcttga ccccgaaatg
     2701 gttggggacg ttttgaacgt tatgaaagac ttggcagaac aaggcatgac catgttaatt
     2761 gttactcacg aaatgggatt tgctcgccaa gtagctaacc gtgttatctt tacagacggt
     2821 ggtcaattct tggaggatgg tactcctgaa gagatttttg atcatcctaa gcaccctcgt
     2881 ctcattgaat ttttagacaa ggtcttaaac gtctaataga gcagacacca tactaacttc
     2941 caaaaaataa taaggcaagg aaacaggttt ccttgccttt tttggttact gttattattc
     3001 aaaccagcaa aatagggata ctcttatgac tctctccttc ataagagtgt cccctatgct
     3061 tttttatggg cgtttatgtt tagtgaggta ataacctaat cccgtaagag ctagtcccgt
     3121 cactatgtgt aagtatttgt taccaaccat cggactaatg gcaatgagaa aaaagccaac
     3181 agccattaaa aaagttgctc catttttttc tgtcatgcaa ggtcactcgc tttttcttta
     3241 ttggccaaaa tgacaaatgg tgcccagata aggaaggcaa caaagaggtt aaacagtgaa
     3301 accaaacctg ccataatatt acctccagta gctaggtaag cataaagtcc aacaggggta
     3361 atccaaggta ctgataagaa taccggtgga atcaaaccta ttgcagtagc aaaataagct
     3421 atgctagcac aaattggtgg cacaattagc catggaatca caaaggttgg attcaaaaca
     3481 attggcatcc caaatgtaat tggttcgttg atattgaaaa cacccattgg tgcggacaat
     3541 ttggcaatgg ttttatgctc ttctcgtttt gagaatatga agatagcaat aatcaatgcc
     3601 aaggttactc ctgaaccacc catttgagca taagcatcaa aggacccgcg tgtccaaagg
     3661 tatggtaaat tagcagcgct atgcgctgta ttgtaagcag ctgtattttc tgttaaagct
     3721 accatataaa taccatccat cactggggct aagacattat gaccatgcaa gccgaagaac
     3781 cagagcagtt gaactaagaa ggttaacaag ataactgaac caataccctg tgataagcct
     3841 agcaatggta attggatata ggttgaaatg acatcactta gggaagaacc tgttaatgcg
     3901 aaaatgaggt aggcgaaaat tgccgaagcg taaatcgcaa ctgttcctgg aataatggca
     3961 gcaaaggctt tattaaccgc aggtggtaca ttatctggta aggtaatggt aatattacgt
     4021 ttggtcaaag ccgcatacac aaaacttgat aaaaagccaa taattaaagc tgtgaacaag
     4081 cctgtggcac cgacatactt tagagctatt gctccccatt gactagtttc aatacttgaa
     4141 gctccacccg cagtaacgac tgaaagcccc atttctttca aattgctaat taggttagca
     4201 gaggcatttt caagtggtgt cgaaatggtt aaagtctgag gtagggtggc aataaatgag
     4261 ccaaaagaaa tcaatcctcc tgccaatgga ttaatcttat gcataacagc taaattgtaa
     4321 ccgaaagcaa aggcaaaaaa gagcgccatg ataccaattg tcccaaaata aacatagcca
     4381 ttgatgtcga taacaggttg catagctttt gcaaatcctg tccatcccat attgtttgga
     4441 atatcgcgta aaaaaacgtt caataagaca gcaactgaac ctgccatggt aataggcatc
     4501 attgaaataa aggaatcacg aatagctact aaatgccgtt gtgacccaat ttttcctgaa
     4561 atctccataa acttatcaga aaaatgcgac ataaatcttc tcctcttttt atttttttaa
     4621 tgtaacaaag gtaaccttaa aagcagcggt gtctgcctaa cacaattatc tcaaaatcat
     4681 ccttgttaaa cgtgatgctt agagataacc ggtaatcggc tcttatccaa atgaaccctt
     4741 ctctctcatt caaggtctgt gaataatctt cacaaagacg atggttgctg caactgttta
     4801 gcatattgga taagggctgc taactgagct tgagattggc gttctaggct ttttttgaac
     4861 agtttgccaa caataaattg atttgctttt tgaaaaatat ctagaggatg atagtcataa
     4921 gaataggtaa ttctagtccg attctccccc agagcctcta attgataagc caattccttt
     4981 atcccatgat tggaactaaa acaagcagta tataattgcg gagcttctag ggccgtaata
     5041 agcaccctca ctgtacgttc tttgtattta ccataatgtt taacatagtt tatccccgct
     5101 ttgatatcat ctcttgacaa ctgtctcaag gtattttgtc gataatcttc ttggagcgat
     5161 atcagcatgg cttcaaaaca atgatgaatg gaaacatctc cttctgctga aataatcatg
     5221 actcacctct ttgtattgct gacattttgt cgtaaacggt aaccaattcc ttggctaaat
     5281 caataaaggc taaggcagtc atcatgtgat cttgcccatg caccatcaat aaagacaatt
     5341 caaccgcatc accagctgct tcttgggaca gcaaatgtgt ctgcgattta tgggcaatat
     5401 ttaacgagtt tgtcgcttct tgaagtaagt gctgagcctc ctcaaactgc ccatctcgtg
     5461 ctgcatgaat agcctctacg gccttgccct tggcttcgcc tccatacatg attaagccca
     5521 taatagcttc taagttggat ggttccgtca tgcctactcc tccatcattg ctagggctgt
     5581 ttcaaggact ttttcaccgt tcataagacc ataatcagcc atgttaatgg catctacttt
     5641 aatatctggt tcaaacttat ctttaaaatc ctttagtaag aatttgactt gtggacctaa
     5701 caagagaacg tcaatgctat tagcagccat ttcgttgtca gcttcactca caggaactgc
     5761 ccaaatagaa acttcaaggt cacgcgcttg ggctgctttt tgcatcttag tcaccaacat
     5821 actagttgac ataccggcat tacaaactaa cataatagat ttcataaggt ttctcctttt
     5881 aatcgtttta atcatgaata agtggggtaa acactgtcat gaatttggaa taatttggtt
     5941 cttctagtaa cgcttgctga agctccacct gattgacaaa agatgccaaa cctggtgata
     6001 catattttaa cctatgatta tgccctttag acggggataa tagaaagaca aaatgaacag
     6061 cttgatgcgc cttgtcccac tcaagaggct ctcggcaaat aaccactact acctgttcag
     6121 aataagtcat gggattagca ggatgcggaa aggcaagcac ttctccatag atgactgggc
     6181 tgtaattttc cctcagtacc atttgatgat agaagtcctc tacaaacgtt tctgttcccg
     6241 cctcgtcaag acaagcaatc atccgtaaaa gcaagtcttc ctttgagatt ttctcgtcta
     6301 aataaagaaa acgattaggg ctaaaaacac ctgttagaag ctgctctgct tttgctaaac
     6361 tcatctgtga cggtaaactg acctctttct tgataccttc ttgttctcca ataaattgtc
     6421 gaatagtttc aatatcctga tttgacaaaa aaacactgac agtaatcact ggtgttaaaa
     6481 acatcagatt ggcaaggctg atagaggaaa taatcaaatc aacggttttc agtcgctctt
     6541 cggttatttc atagtaactg ataacatcga caatacgcaa acgtccttca aattcctttt
     6601 ccaagcgatt tttcaacatc atggcactac cataacctgt ggcacaaact actagaaccc
     6661 tcaatttatg acggtttgaa taacgctcaa tggccgccat taaatgcaaa ctaatataag
     6721 cccattcatc atctgagaca tcattctttt gacatacaag caaatccgaa aaggtctgct
     6781 tggtaagggt aaatatttca ggatactggc ttttaatttc ctcagttaaa ggattagtca
     6841 gctgaatatg gttttcaaga cgtatggtca agggcatcat gtgggctaat aaaccttgaa
     6901 ataaattggt atctgtttct aaagtcatgc ctgtcaattg actaatcttg gcaatacatg
     6961 cttttaaatg gtcctgcaac ttttcatcgg tattatcatc ttgccaatgc ttaccaacag
     7021 aatgtttgac ctttagatgc agtgcaatat aattagcctc ctctttagga aaacgtatcc
     7081 ccatcacttc ttccactcga tacaaaatgc gcagagctac ttgatactca tcagactgac
     7141 gaatagctat tggaatggaa aacagttcta atggacaccc cgatcgaata cgctgaacca
     7201 ttagggcaat atgcaagact agattattca tgacaaaatc agaaagtttc aacttagcat
     7261 cccgacattc atccaagaca ataatcatga tttctgcaaa attaatacct tcaagaaagg
     7321 tattacctac catggtggac aaggtattat caaaactagt aacaaaaaaa tagtccatga
     7381 taaaatgcct aatgtcctct tctttacctc ttatttgaat atgttggtga gttatttcaa
     7441 gctctaactg gtaaggtatt atgcgctcct taatacctgc taaaacatgt tttaaagttg
     7501 tccgactgat atacaattct tgacaaagct cttcaaaatc ctgaacggca tcttcaaaaa
     7561 agaatttatg aagcacataa tcttctcgat caaccgattc ttccacttga gtcacatctg
     7621 ccaaacgttt tttagacgtc acactttctt gccaaaagat gtcaaatgcc atagaatggt
     7681 caatttctaa gcagtaacct cgaccttgtt tagaaatgat gtgagctccg tgttcaggaa
     7741 ggctactgat cagttctttg aggtatttcc taaccgtacg gtcactcata ccgataacct
     7801 cagccagagt tgcactagag acaaactgct ctttgtgatg gattagaaat gttatgatgg
     7861 ccagttcttt ttttgataac attagtcctc cttactactt tagaaaacgc tttctaccct
     7921 cctttagtgt accaaatatt tcctgaaaaa ggtgaccatc attttccgca ttgattgtga
     7981 aaagaatcta tgatggggca atctccaaac aaaattcctg ccaaggtcgc aaatcatcta
     8041 acaaactaag atagtcctct ctgacctcag caaccacatt agcttttgaa ctgaccgtaa
     8101 aatttttcaa agcaatttgc agttctccct tatatcgatg atagcggtca ttgtcaataa
     8161 taatgctacc tcgtttgacc tccttagatt ggtctcttgg agcaatcgat tcctgagcat
     8221 agactagacg tggcatggtt gatcgaatca cataatcaga tacatcgcca cgataacaat
     8281 gcggatagcc aatgatttgc tcctcaactt ctgtcaaatc gatgataggc ctaaccttga
     8341 tcgtcgtgac tggtcttgct aaagcttgag tacatgctgc caattcttct tccgaaatga
     8401 attggttaga tatgaggata ttatcaatgg ttcctatcgc tttcattaat tctacttgca
     8461 ggccaatggg caagtgccta tggtcttcca ctgtcggcaa gccttctgcc agtagccaag
     8521 ggccttcaga tgctgactga gcggtaataa aagcagccgt ttcaatatca tgctcatgat
     8581 aaaaacgtga catatcctta aaatgttgcc atgacaaacc ggtaaattca tgtggataaa
     8641 aattatggca accaataata ttgctccgtt ctgcatctgt cgctagcaat gacatcaata
     8701 attgtttatc agtgctcata ttaagttcaa ttttaagccc aaagggattt cttgttaaag
     8761 ttacaatctc tgctagaggg agagcctcgt ctaagcgtaa gcccgctaac ccgaaggcat
     8821 gagctcgttc aataagctga tctgaccaac cagcttggct aataaagctt ggactaacgt
     8881 cagcaatcac tcgaatgcct aattgatttg cataagcaat gagctctgcg tagcaatgaa
     8941 acatctggtg atcagctggt gccagctgta agagactcat aaacaagcgc ttggctccat
     9001 agctatgaca taagtcaata taagctttac tcttggtaac atcataccgc tcagggtata
     9061 gagaaaatcc taaatcaacc atattgcctc ctatttatca aaaccatttg actcgctcaa
     9121 cgccttaaac cagtaacctg atttttttat ggttcgcttt tgactctcta aatccaaagc
     9181 taccaaacca tatcggtttt tataagcatt taaccaagac cagcaatcaa taaaagtcca
     9241 caagaggtag cctttgcagt tggctccttc tcccaaagcc ttatgaagct ggataagatg
     9301 atcctcgata aaggtgatgc ggtagtcatc ttgtatctgg ccatctgcta aaaaagcttc
     9361 ttcaccttcg acacccatac cattttccgt gactagccat tcgatgttac catagtgttc
     9421 ttttaaatta agggcaatat cataaagtcc tggttcataa atttcccaac cccggtgagg
     9481 attgactttt ttaccaggca tatcataagg ttcaaaataa atatctaaga taaggaggtc
     9541 cccctcttgc tgtgacttac taggtgcctg aacccgcaaa ggctgataat aattaactcc
     9601 caaaaaatca acagtattgt ttttgataat ctctaattca tcagcactat attgaggtaa
     9661 taaatcagct tcctctaaaa tggaaactaa ctctgctggg taaacaccca aaactgatgg
     9721 atctaggaag gatttggttt ggaaaagctc tgcgattcta gcagccttga catcttcagg
     9781 agcattactc cgaggataag caggtgtcat gttaagcaca atactaatct tgtggtcggg
     9841 atgaagctta tgacaagctt tgaccgccag tgaactggcc agttgtgtgt tgtaagccac
     9901 ttgaactgct gccttggcat ctactttaca agggtagtga taataaccta aatagccaca
     9961 ttcgactgga acaatcggtt cattaaaggt aatccaagtg tttactaaat caccaaaaag
    10021 ctcaaaacag gtttttgcgt aggtttcata agcccagaca gtagctttgt tttcccagcc
    10081 acctttttct tgcaaggcat aggggaggtc aaaatgatag aggttaacaa tcaatttaat
    10141 accttgagca ataatgtctt gaaatacttc tcggtaaaat gtcaccgcct ttggattaac
    10201 ctccccgact ccttctggaa tcaatcgaga ccactggatt gaggtccgaa aaatcgtgtg
    10261 gcctgtttct ttcaaaaggg caatgtcacc cttatagttt tcataaaagg ttgaggtttt
    10321 ttctggacca atttggtgat gaaacttagc tgactcaata ctaaaccagt aatcccagtt
    10381 gctaggtcct ttgccatctc caggaaccgt tccttcgctt tgaggcccag atgtggaact
    10441 tccccataaa aagccatctg gaaattgata acggtgtttt ctttgtttca tatcctttgt
    10501 cctttctttg ctcacttctc tttttattat aagaagaaaa acaggtgatg taaacgcttt
    10561 aaaaaatctg ttttttcctt atcatcacgg aaaataagtg atttaaagct tgacatattt
    10621 tattatctta aagagcctat ctaacctgcc ctgctacttc ttatcaaaac caagccccta
    10681 ttgacagatg ccttagccta tgtttgaata ggggtatggc ttatttaaac tactttacaa
    10741 aaaccgagtg ggcactttgg ttaagctctg tcttggccat tttaattagc gcccttttat
    10801 tcggtaagca agctccctta gctttaatag cttctttgat tggtgtgacc tctctcattt
    10861 ttagtgctaa agctaatcct attggtcaag ggctcgtcat tattttttcc attatctatg
    10921 cctatctttc attacgaaac agttactatg gagagttaat gacctatctt tttatgaccc
    10981 tccctatgac tattttttca ctttttactt ggttaaacca tccttttgaa gggaaaaaat
    11041 cacaagtgac tatttcacga ttaacaccca ctgatcgaag atgtcttttc gtttttacta
    11101 tactgatcac tcttattttc tacagcatct tggcttctat tcaaacagcc tatttacttg
    11161 tctccacttt atcgattgcc acttctttct cagcggttta tctcagctat aagcgcagcc
    11221 cttattttgc tttagcatac gggctcaatg atctagtcct cattctctta tggatgcatg
    11281 ctgctcaaac agatgccagc caatatgctg tggtcatctg ctttgccacc tttcttatta
    11341 atgatagtta cactttttat aattggctaa tgctccaacg ttaccaagaa aaaaaggtca
    11401 aaaaaggatg agggcctaag cctcatcctt tttcaatttt tcacgttcta agcgcaaacg
    11461 tctattggga tttaaaagat taaacaattc ttctaatttc tcagcattcc aaacatctgc
    11521 tgccgataca aaatttccat ccttatcctt aaaggatatc ggtttatcac ccataatcag
    11581 aaacctccat cttaaagata acacacaaga gcactatcac actgctattg taatagattg
    11641 ccttaatcca caaattcaaa ttcaaacttg ccaatacgaa caggatcccc atcttttgct
    11701 ccacgttcac gaagagcttc gtcaaccccc ataccgcgca attgacgggc aaatttcatg
    11761 atggactcat cacgttccat attggtcatg acaaagagac gttctaactt ctcaccagac
    11821 aagacccaag tagcgtcatc gtcacgtgtg atctcaaagt ctttttcagt ctcagcaaag
    11881 ccatagtaag cttcctcgtc caccagatca gattcatcat ataataagaa ttcgtctgtt
    11941 ttagcgagta attctgccgt tgcttctaac aagttctcta agccttgatg tgctaggctt
    12001 gaaataggga aaatcattgg caaatcatca aactcgtcat attgggcagc caattttttc
    12061 ttgaaggctt tcaagttttc ttgagcctct ggtatgtcca ttttattagc cacaataatc
    12121 tgtgggcgct ccatcaatcg caaattatag gtttccagct cgttattaat ggaaacataa
    12181 tcttcgtaag gatcacgacc ctcactagca gacatgtcaa tcacatgaag aataacccgt
    12241 gtccgctcga tatgacggag gaattgagtt cctaaaccaa ccccttggct agcaccttca
    12301 atcaaacctg gcagatctgc catagcaaag ctatcacctg actttgtacg aaccattcca
    12361 agattaggaa caatagtggt aaaatgatag gcaccaattt tcggtttagc tgatgacaca
    12421 acacttaaca aggttgattt cccaacagat gggaatccga ccaagccaac gtcagctaag
    12481 attttgagtt ctaactctaa ctgacgttcc tcacctggct caccattttc agcaatctct
    12541 ggcgcaggat tacgaggagt tgcaaaacga atattcccac ggccaccacg accaccctta
    12601 gcaatgacca cttcttgacc atgttctact aagtctgtaa ttacttttcc agtttcagca
    12661 tcacgaaccg ttgtcccttg aggaacaaag acaatcaaat cttctgcacc acggccgtgc
    12721 atgcctttgg tcataccttt ttcaccagat ttcgccttga atttacgatt ataccggaaa
    12781 tccatcaagg tacgcaaacc ttcgtccact cggaagataa ctgagccacc tttaccgccg
    12841 tctcctcccc aaggaccgcc atttgggacg tacttttcac gacgaaaagc taccatacca
    12901 tcgccaccac ggcctgcttg gacactgatt tttgccgtat ctaaaaacat actcatactt
    12961 tttttattct ctaactttct tttactttaa aaaaacgcct ttcagcgttt ccttacatta
    13021 atactgatag ggcactcgca ataagcccgc caacagttac aagaaccatt aggataacaa
    13081 caaacattgt cactttttca aaggttgttt ttttgcgagg tccattttct ccaaatgcca
    13141 cattaaatac ctcatctatt gatttttctt cttatatctt aacatgaaag atcgttttag
    13201 gcaaatatca gatgaatatt tacgctatgt aggctattac tgagtattgt atcttgtgaa
    13261 tttcatatga atatgataca ttagcatttg caaaataatg ttacagataa aaacctcctc
    13321 gtctg
//
LOCUS       DAXWIQ010000044        13188 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000044 DAXWIQ010000000
VERSION     DAXWIQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13188
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.044"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(7..1275)
                     /locus_tag="ACGEUI_001415"
     CDS             complement(7..1275)
                     /locus_tag="ACGEUI_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFF8166244.1"
                     /translation="MTTKYQTIISNIEQDIQKQRLKKGDKLPSIRVLSKVYYCSKDTV
                     QRALLELKYRHLIYAVPKSGYYVLGNVSMPDNVLNLSLEDYNNMAYEDFRLCLNEALS
                     AKDKYLFHYYHKTEGLEELREALLLYLAENSVYSNKDQLLITSGTQQALYILSQMPFP
                     NTGKTILLEKPTYHRMEAIVAQLGLPYQTISRHFNGLDLELLESLFQTGDIKFFYTIS
                     RFSHPLGLSYSTKEKEAIVRLAQRYQVYILEDDYLGDFVKLKEPPIHYYDTHHRIIYL
                     KSFSMSVFPALRIGALVLPSGLKPHFLTQKSLIDLDTNLLMQKALALYLENGMFQKNL
                     RFIKRYLKQRERQLALFLKQNCPDIHYQLTPTHLVIDYTTSDSYRNFTLDKSDRIIIT
                     GKKRYLSITINQQIQSKLNSLIKNTCGKSN"
     gene            1365..2231
                     /locus_tag="ACGEUI_001416"
     CDS             1365..2231
                     /locus_tag="ACGEUI_001416"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HFF8166245.1"
                     /translation="MKRIVVANDLVGVGKVALSASIPLMASCLLEQVLLPTCLLSSHT
                     GGGQNPVKHTTSSILDVFLTDWDNKRIKFDGIFIGYFTTTRDGQKLLEWLSRKQLPLI
                     LDPIMADNGKLYQGFSQKHIEIMIKLTQKAELVLPNISEACLLTQTAYLAKNYDEEDI
                     RQVARKLAKLGPKQVVISGISFEEGSIGFAYYQSDSDQLTFHFGKQFPYHFYGTGDIA
                     SAIITAGFYYQLPLTQILDLVVSFLEKVLQNTLTLKRDLILGVAYESHLNYLTKSFKK
                     LLEEKNVISKIS"
     gene            2209..2766
                     /locus_tag="ACGEUI_001417"
     CDS             2209..2766
                     /locus_tag="ACGEUI_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8166246.1"
                     /translation="MSSQKLANYIELSMYAALIFISVQVLRVPVGMQFIHLGNALVVI
                     AVLIYGSKKGALVASLGLGIFDVLNGYASVVWITILESLAICLILHLVYERAMQSSKK
                     PKAIITITVIAALSKLTINLIKYLLLAYIGTQLPLSVSFWVALGKIGGSFGTSFVTLV
                     AVPLLYPVIKNLKEKVTIGQAKRGF"
     gene            2900..4444
                     /locus_tag="ACGEUI_001418"
     CDS             2900..4444
                     /locus_tag="ACGEUI_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8166247.1"
                     /translation="MSILEVKQLSHGFGDRAIFENVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGHLQPDEGKVEWSKYVTAGYLDQHTVLESGQTVRDVLRTAFDELFKTENRIN
                     EIYASMADDKADIAVLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMESDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNHLDAEHIEWLKRYLQHYENAFVLISHDI
                     SFLNDVINIVYHVENQSLVRYTGDYYQFQAVYEMKQSQLEAAYERQQKEIANLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPNFEFKQARTPSRFIFQTKNLVIGY
                     DYPLTKEPLNITFERNQKIAIVGANGIGKSTLLKSLLGVIEPLEGHIVTGDFLEVGYF
                     EQEVTGVNRQTPLEVVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQAKVR
                     FCLLMNRENNVLILDEPTNHLDIDAKNELKRALKAYKGSILMVCHEPDFYNGWVTDTW
                     DFSKLT"
     gene            4809..6032
                     /locus_tag="ACGEUI_001419"
     CDS             4809..6032
                     /locus_tag="ACGEUI_001419"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HFF8166248.1"
                     /translation="MTNLTFYAKIGISEEEHDSFVKEHQQISVLQGSDWAKIKNQWQN
                     ERIGIYKEEKQVASLSLLIKLLPLGRSIIYIPRGPVMDYLDRDLVAFTMKTLKDYGKT
                     KKALFIKYDPAILLKQYALGQEEEEKPLALAAIKNLQEAGVHWTGLTMEIADSIQPRF
                     QANIYTQENLEMQFPKHTRRLIKDAKQRGVKTYRVSQSELHKFSKIVSLTEKRKNISL
                     RNEAYFQKLMTTYGDKAYLHLAKVNIPQKLDQYRQQLILINQDITRTQAHQKKRLKKL
                     EDQKASLERYITEFEGFTDQYPEEVVVAGILSISYGNVMEMLYAGMNDDFKKFYPQYL
                     LYPNVFQDAYQDGIIWANMGGVEGSLDDGLTKFKANFAPTIEEFIGEFNLPVSPLYHI
                     ANTMYKIRKQLKNKH"
     gene            complement(6074..7636)
                     /gene="guaA"
                     /locus_tag="ACGEUI_001420"
     CDS             complement(6074..7636)
                     /gene="guaA"
                     /locus_tag="ACGEUI_001420"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFF8166249.1"
                     /translation="MTEISILNDVQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TAQELREINPIGIVLSGGPNSVYADNAFGIDPEIFELGIPILGICYGMQLITHKLGGK
                     VVPAGQAGNREYGQSTLHLRETSKLFSGTPQEQLVLMSHGDAVTEIPEGFHLVGDSND
                     CPYAAIENTEKNLYGIQFHPEVRHSVYGNDILKNFAISICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHGLLRKDEGDQVMGMLG
                     GKFGLNIIRVDASKRFLDLLADVEDPEKKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGIALGMPEEI
                     VWRQPFPGPGLAIRVMGAITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            7803..9236
                     /locus_tag="ACGEUI_001421"
     CDS             7803..9236
                     /locus_tag="ACGEUI_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166250.1"
                     /translation="MLRKKRILGLFRLVELIFLGLLLSLVVSYLAWTNSFATLHNILA
                     TVGIVERSKDQQPRYHIGQAIQVQKSGPYHQWIGTINKQVEDIAENYRVSYHYEVVFP
                     IGKVTVSLPEHNLKEPDKPRFKKGDIVKLSSLTKKPHIKVYQGQLATIKQVKKCYDYS
                     LGGYQYDINLKDNLRLDGISEQDFVKPYYIRFNKGNSPEQNNRLLRKAFAYAKQHPNS
                     VISFPKGQFHIGSLPSQKDYFELPSDTAIIGHQTEFIIHGKMLWFGFPTGPKAEQGVR
                     NLVLTGVHFKANDLKKGDHFMIMADHGTDWHIYDNKFTMVHKRNSHIFDLGSLQNSLF
                     EKNQFIGYAPELVQDQQLLSKAQGHDFFSEVIQFDAAVHHFAWDGGLLSNIAPNYEAF
                     NQTRHLCHNITVSQNQFLPYIDPTGCLRAYSGSIGQHSSKVGVIRVLNNVFTSSIVTK
                     AKLTSWFMEPIHFPPNSPVIVAGNIIN"
     gene            9319..10017
                     /locus_tag="ACGEUI_001422"
     CDS             9319..10017
                     /locus_tag="ACGEUI_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8166251.1"
                     /translation="MLPAYIKIHDAIKKDIDLGIWPIGSRLPSERHLAEHFTVSRMTL
                     RQAITLLVEEGILERRIGSGTYVASHRVQEKMRGTTSFTEIIRSQGRQPSSKLLSYQK
                     QLASDTEVKELNLDKTDLVIRMERIRYADSVPLVYEIASIPEKFIKTVKRADITEHFF
                     HSLIANGYEIGKSKQTIYAKLASERVASYLEVAKGHAILALTQVSYFTDGKPFEYVRS
                     QYIGDRFEFYLENN"
     gene            10115..10447
                     /locus_tag="ACGEUI_001423"
     CDS             10115..10447
                     /locus_tag="ACGEUI_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFF8166252.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIADE
                     FGVSRQAVYDNIKRTEKILETYEMKLHMYSDYVVRSEIFDDMIAHYPHDEYLQEKISI
                     LTSIDNRE"
     gene            10462..12024
                     /gene="ffh"
                     /locus_tag="ACGEUI_001424"
     CDS             10462..12024
                     /gene="ffh"
                     /locus_tag="ACGEUI_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFF8166253.1"
                     /translation="MAFESLTQRLQDVFKHIRGKKKLSESDVQEVTKEIRLALLEADV
                     ALPVVKTFIKRVRERAIGHEIIDTLDPTQQILKIVNEELTSILGSETAEIDKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEENARPLMIAADIYRPAAIDQLKTLGQQINVPV
                     FDMGTDHSAVDIVRKGLEQARENHNDYVLIDTAGRLQIDEKLMGELRDVKALAQPNEI
                     LLVVDSMIGQEAANVAYEFNHQLSITGVVLTKIDGDTRGGAALSVREITGKPIKFTGI
                     GEKITDIETFHPDRMSSRILGMGDLLTLIEKASQEYDEKKSLELAEKMRENTFDFNDF
                     IEQLDQVQNMGPMEDLLKMIPGMAGNPALANIKVDENQIARKRAIVSSMTPAERENPD
                     LLNPSRRRRIAAGSGNSFVEVNKFIKDFNQAKSMMQGVMSGDMSKMMKDMGINPNNLP
                     KNMPAGMPDMSSLEGMMGQGGMPDLSGLGGDMDMSQLFGKGFKGKIGQFAMKQAMKRQ
                     ANKLKKAKKKRK"
     gene            complement(12141..12839)
                     /locus_tag="ACGEUI_001425"
     CDS             complement(12141..12839)
                     /locus_tag="ACGEUI_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFF8166254.1"
                     /translation="MFSGHQLKTARLSKGITQSELGRLLHVNKMTISNWEKGKNIPNE
                     KHLNALLHLFNVTSDYFDPNYRLLTPYNQLTISNKEKVIGYSERLLNHQIDKKSKDLI
                     DKPSQLYAYRVYESLSAGTGYSYFGDGNFDVVFYDEQLEYDFASWVFGDSMEPTYLNG
                     EVVLIKQNSFDYDGAIYAVEWDGQTYIKKVFREDEGLRLVSLNKKYSDKFAPYSEEPR
                     IIGKIIANFRPLEI"
BASE COUNT         4359 a         2184 c         2515 g         4130 t
ORIGIN      
        1 gaaaagttag ttgctctttc cacaagtatt tttgattagt gagtttaatt tgctttgtat
       61 ttgttgattg atagtaatag agagatagcg tttttttcca gttattatta ttctatcaga
      121 tttatctaat gtaaagtttc tataagagtc tgatgtcgtg taatcaatca ctaaatgagt
      181 aggggttagt tgatagtgaa tatcagggca attttgtttt aaaaagagag ccaattgacg
      241 ttcccgctgt tttagataac gcttaataaa gcgaagattt ttttgaaaca tcccattttc
      301 taaataaaga gctaatgctt tttgcattag taagttcgtg tctaaatcaa tcaatgattt
      361 ttgggttaaa aaatgaggtt ttaaacctga cggtaaaact agagctccga tacggagtgc
      421 aggaaaaaca ctcattgaaa aagattttag gtagatgatt cggtgatgag tgtcataata
      481 atgaattggt ggctctttaa gtttcacaaa atctcctaaa taatcatctt ctaaaatata
      541 aacctggtat cgttgtgcta gacgaacaat agcttccttt tccttagtgc tataagaaag
      601 tcccagagga tgtgaaaatc gtgaaatcgt gtaaaaaaat ttaatatcgc cagtttgaaa
      661 taaagattct aagagttcta aatcaaggcc attaaagtgt ctagagatag tttgataggg
      721 taatcctaac tgagcaacaa tagcttccat ccgatgataa gtaggctttt caaggagaat
      781 tgttttgccc gtattaggaa aaggcatttg tgataaaata taaagcgctt gttgcgtccc
      841 agaggtgatt aatagttggt ctttgttact gtaaacacta ttttcagcta agtaaagaag
      901 caaagcttcc cttagttctt ctagtccttc ggttttgtga taataatgaa agaggtattt
      961 gtctttagca cttaaggcct catttaagca gagtcggaaa tcttcataag ccatattatt
     1021 ataatcttca agacttagat taagaacatt atcgggcatg cttacgttac ccaaaacata
     1081 ataaccactt ttaggaacag cataaattaa gtgacgatat tttaattcta aaagagcccg
     1141 ctgaacagta tctttgctac aataataaac cttacttagt accctaattg aaggtagctt
     1201 gtcccctttt tttagtcgct gtttctggat atcttgttcg atattggaaa tgattgtttg
     1261 atattttgtt gtcataaatt gtccccatac agttaagtgt tataactatt gtatcttatg
     1321 cctctttttc ttacaatagt tattatagaa aagaggtaat agctatgaaa cggattgttg
     1381 tggcaaatga tctggttggt gttggtaagg tagccttgtc tgctagtatt cctttaatgg
     1441 cttcttgttt gttagagcaa gttttattac caacttgcct tttatcaagc catactggcg
     1501 gaggacagaa ccctgtgaag catactacat caagtatatt agacgttttt ttaacagatt
     1561 gggacaacaa aagaattaag tttgacggca tttttatagg ttattttacg acaacaagag
     1621 atgggcaaaa gctgctagaa tggctttcaa gaaagcaact tcccttaatt ttagatccaa
     1681 ttatggcaga taatggaaaa ctttatcaag gcttcagtca aaaacatata gagattatga
     1741 taaaacttac tcaaaaagct gagcttgttt tacctaatat tagtgaggct tgtctattaa
     1801 cacaaacagc ttatttagca aaaaattatg atgaagaaga tattagacag gtggctagaa
     1861 aattagctaa gcttggacct aaacaggttg tcattagcgg aatctctttt gaggagggca
     1921 gcattggttt tgcttactat cagtcagatt cagatcaatt aacattccat tttgggaagc
     1981 aattccctta ccatttctat ggtacaggag atatagcatc agctattata acagcaggat
     2041 tctattatca gctaccacta acacaaattc tagatcttgt tgtttcattt cttgaaaaag
     2101 tattgcaaaa tacccttaca ttaaaaagag atttaatctt aggtgttgct tatgaatctc
     2161 atttaaatta cttaactaaa tcgtttaaaa agttgttgga ggaaaaaaat gtcatctcaa
     2221 aaattagcta attatattga attgagtatg tatgccgctt taatttttat ttcagtacaa
     2281 gtgttgcgtg tccctgtcgg tatgcagttt attcatcttg gcaatgcatt agttgttatt
     2341 gcagtcttaa tctatggtag taaaaaagga gctctagttg ctagtcttgg tttagggatc
     2401 tttgatgttt tgaacggcta tgcttcagtc gtttggataa ctatcttaga atccttggct
     2461 atttgtttaa ttttgcattt ggtatatgaa agagctatgc aatctagtaa aaaaccaaaa
     2521 gccattataa caataacagt tattgctgca ttgtcaaaat taactattaa ccttataaaa
     2581 tacttactgt tagcttatat aggtactcag ctaccacttt cggtatcttt ttgggtagct
     2641 cttggaaaaa ttggtggtag tttcggaaca tcttttgtga ctttagttgc tgttcctctt
     2701 ttgtatcctg ttatcaaaaa cttaaaagaa aaggttacaa tcggacaagc aaaaagaggt
     2761 ttttgataca agtaccttat cgataaaaaa gcaaggtaca cttttagtca aatattgaca
     2821 aaaggttaca attttgccat aatgaaataa ctataaagcc gttttgacgg cttttttgat
     2881 gacagttaag gaagaaacga tgagtatttt agaagttaag cagttgagtc acggttttgg
     2941 ggatcgggct atttttgaaa atgtgtcatt tcgcctctta aaaggcgaac atattggact
     3001 agttggggca aatggtgaag gaaaatcaac ctttatgagt atagtcacag gacatttaca
     3061 gcctgacgaa ggaaaagtag agtggtcgaa gtatgtcact gcaggttacc tggatcaaca
     3121 tacagtgttg gaatcaggac aaaccgttcg tgatgtcttg cgaactgctt ttgatgagtt
     3181 atttaagacc gagaatcgta ttaatgagat ttacgcgtca atggcagatg ataaagctga
     3241 tattgctgtt ttgatggaag aagtaggtga gcttcaagat cgtttagaaa gtcgtgattt
     3301 ctatactttg gatgctaaga ttgatgaagt agcgcgtgcg cttggtgtta tggattttgg
     3361 aatggagtca gatgttacat ccttatcagg tgggcaacga acaaaggttt tattagccaa
     3421 attactatta gaaaaacctg atattctgct attagatgaa ccaactaacc atttggatgc
     3481 tgagcatatt gaatggttaa aacgctattt acaacattat gaaaatgctt ttgtgttgat
     3541 ttcgcatgat atttctttct taaatgatgt gattaatatt gtttatcatg ttgaaaatca
     3601 aagtttagtt cgctatactg gggattatta ccaatttcaa gctgtttatg agatgaaaca
     3661 atctcaactt gaagcagcct atgaacgtca acaaaaagag attgctaact tgcaggattt
     3721 tgtcaaccga aataaggctc gggtagcgac acgtaacatg gcaatgtctc gccaaaaaaa
     3781 acttgataag atggatataa ttgaacttca agctgagaaa ccaaaaccaa attttgaatt
     3841 taagcaagct agaactccca gtcgattcat ttttcaaaca aaaaatcttg tgattggtta
     3901 tgattaccca ttgaccaaag aacccttaaa tataacgttt gaaagaaatc aaaaaattgc
     3961 tattgttggg gccaacggta ttggaaaatc tactttgcta aaaagtttat taggtgttat
     4021 tgagccttta gaaggtcata ttgtcacagg ggatttttta gaagttggct actttgaaca
     4081 agaagtgaca ggtgttaacc gacaaactcc gctagaagta gtttgggatg cttttcctgc
     4141 cttaaatcag gcagaagttc gagcggcact agctcgttgc ggactaacat caaaacatat
     4201 cgaaagtcaa attcaagtac tttcgggtgg tgaacaagca aaagttcgtt tttgtttgtt
     4261 gatgaatcgt gaaaataacg tgcttatttt agacgaacca acaaatcatc ttgatattga
     4321 tgctaaaaat gagctcaaac gtgctttaaa agcatataag ggttctattt taatggtttg
     4381 tcatgaacct gatttctaca atgggtgggt aaccgatact tgggatttta gtaagttaac
     4441 ctaaactatt ccttaatttt ttacacagaa attactaaaa tcagtaaaga tatagaataa
     4501 atactgctag atatgtgatg gtttaggtaa ctgatcacca accaaaaaag atgtgactac
     4561 atcttttttt tatctaatgg aatggtgtaa agtcgtttaa taatagctta ttttttgatg
     4621 atcagttgtt catgaaattg ttggtgatta acaagtcatt taagatacaa acgttattac
     4681 taaaaagtga ttcaaatagt ttccctattt ttatgttttt ttaaaattta ttacgaataa
     4741 atagataaag acagtaaaga tgattagtaa gtacatctgc taagattacg aatagatggg
     4801 gacaataaat gacgaattta acgttttatg caaaaatcgg tatttcagaa gaagagcatg
     4861 actcttttgt taaagaacat cagcaaatta gcgttttaca aggtagtgat tgggcaaaaa
     4921 tcaaaaatca atggcagaat gagcgaattg gtatctataa agaggaaaag caggttgcct
     4981 ctttatcact tttgattaag ctattgccac ttggaagaag cattatttat attccaagag
     5041 ggccagtcat ggattatctt gaccgtgatt tggtggcatt taccatgaaa acactaaagg
     5101 attatggtaa aactaaaaag gccctcttta tcaaatatga tccagctatc ctgttaaaac
     5161 aatacgcact gggacaggaa gaagaagaaa aacctttagc tttagcagct attaagaatc
     5221 tccaagaagc tggtgttcat tggactggtt taacaatgga gattgcagat agtatccaac
     5281 ctcgtttcca agctaatatt tacactcaag aaaaccttga gatgcaattt cctaagcata
     5341 ccagacgttt aataaaagat gctaagcagc gtggtgtaaa aacatatcgt gtcagtcaat
     5401 cagaacttca caaattttcc aagattgtct ccttaacaga aaaacgtaaa aatatttctt
     5461 tgcgtaacga agcttacttt caaaagttga tgactactta tggggataag gcctacttac
     5521 atctagcaaa agtgaatatt cctcaaaaac tagatcaata ccgccagcaa ttaattctta
     5581 ttaaccaaga tattactcgc acccaagctc atcaaaagaa gcgtttaaaa aaattagaag
     5641 atcaaaaagc ttctttagaa cgttatataa ctgaatttga aggctttaca gaccaatatc
     5701 ctgaggaagt tgttgtagca ggtatattat ctatttctta tggaaatgtt atggaaatgc
     5761 tttatgctgg gatgaatgat gattttaaga agttttatcc tcagtatctg ctgtatccta
     5821 atgtttttca ggatgcttat caagatggta ttatttgggc taacatggga ggagtagaag
     5881 gctcgcttga tgatggactt accaaattta aggccaactt tgctccgaca atagaagaat
     5941 ttataggaga atttaatctc cctgtcagcc cactttatca tattgctaat accatgtaca
     6001 aaatacgaaa acagttaaag aataaacatt aaaagactcg ctcattttgg cgagtctttt
     6061 aatgtttatt ctgttattcc cattcaactg ttgcgggtgg tttacttgtg atgtcgtaga
     6121 cgatacggtt aacgtggtca acttcattta cgatacgtgt tgagattttt ttcaagacat
     6181 cccaaggaag ttgagcaaag tcagctgtca taccatcaat agacgtaata gcacgaatgg
     6241 cgatggtata atcataagta cgaccatctc ccatgacgcc tacagaacgg acacctgtgt
     6301 taactgtaaa gtattgccac acgtcacgat caagtccagc cttagcaatt tcttcacgaa
     6361 ggatagcgtc tgattcgcga acggtttcaa gtttttcttc agtaattgct cccatgacac
     6421 ggatagcaag tccaggacct ggaaatggtt ggcgccaaac aatttcttca ggcattccaa
     6481 gagcgattcc aagcgctcga acttcatctt tgaaaagagt gtttaagggc tcaatcaatt
     6541 caaactgcat gtcttcgggg agaccaccca cattgtgatg tgatttgatg gtttgagcag
     6601 tttctgttcc tgactcaatg atatcagtat aaagtgttcc ttgggcaagg aagtcaacac
     6661 cttttaattt gctggcttca tcatcaaaaa catagacaaa ttcattacca ataattttac
     6721 gttttttctc aggatcttca acgtctgcaa gaaggtctaa gaaacgtttt gaagcatcca
     6781 cacggataat atttaggcca aatttgcccc caagcattcc cataacttga tcgccctcgt
     6841 ctttacgaag aagaccgtga tcaacgaaaa tacaagttaa ttggtcaccg atagcttttt
     6901 gaagtagaac accaacaact gaagaatcaa ctccaccaga aagacctaga agaactttac
     6961 gatcgcctac agtttcacga attttagcaa tttccatgtc aataaaatta tccattgacc
     7021 aatcaccacg cgcgccacaa attgatatag caaagttttt aagaatgtca tttccataaa
     7081 cagagtgtct cacttctggg tggaactgaa taccgtaaag gtttttctca gtattttcaa
     7141 tagctgcata gggacagtca tttgagtctc caacaaggtg gaaaccttct ggaatttcag
     7201 taacagcatc accatggctc atcaaaacga gttgttcttg aggtgtgcct gaaaataatt
     7261 ttgacgtttc acgaagatga agggttgact gaccgtattc acgattacca gcttgtccag
     7321 caggaacaac tttaccacct aatttatggg tgattaattg cataccgtaa cagataccaa
     7381 gaatcggaat ccctagttca aagatttcag ggtcaatgcc aaaggcgtta tcagcgtaaa
     7441 cagagttagg ccctcctgat aaaacgatac ctatgggatt gatctcacga agttcttgag
     7501 cggtgatttt atggcttttt agttcggaga aaacaccaaa ctctcgaata cgtctagcaa
     7561 taagctgatt gtactggcta ccataatcaa gaacgataat tttttgaaca tcattcaaaa
     7621 ttgaaatttc agtcattata atcctttcat tctataccaa ccgtcaggtt gatagttgtc
     7681 aacttattgt agcatatttt catcgaaaag acattacaat ttctctagct cgttggaaaa
     7741 aaacgtaatc gttatgttac aatagaggct atgaaaggga attaagacaa cagggagggc
     7801 ctatgctaag aaaaaagaga attttgggtt tatttcgact tgttgaatta attttcctag
     7861 gattattgtt gagtttagtt gtttcgtacc ttgcgtggac caatagtttt gcaacacttc
     7921 ataatatatt agcaactgtc ggaatagtgg aacgaagcaa agatcaacag cctcgctatc
     7981 acattggaca agctattcaa gtacaaaaaa gtggtccata tcatcaatgg attggaacta
     8041 ttaataaaca ggtagaagat atagccgaaa attaccgagt gagttatcat tatgaagtgg
     8101 tatttccaat aggaaaagtc actgtttctt tgccagaaca taacctgaaa gagcctgata
     8161 aaccgaggtt taaaaaagga gatatcgtta aattatcttc attaactaaa aagccacata
     8221 taaaagtata tcaaggtcaa ttagcgacta ttaaacaagt taaaaaatgc tatgactatt
     8281 cgttaggagg atatcagtac gatattaatc tgaaagataa tctaagatta gatggaattt
     8341 cagagcaaga ttttgttaaa ccttattata ttaggttcaa taaaggaaat tcccctgagc
     8401 aaaacaatcg tcttttgcga aaagctttcg cttatgcaaa gcagcatcca aatagcgtta
     8461 tatcttttcc aaaggggcaa tttcacattg gctctttgcc ttcacaaaaa gattattttg
     8521 agcttccatc tgatacagct attattggtc atcagacaga gttcattatt cacggtaaaa
     8581 tgttgtggtt tggattccct acaggaccaa aggctgaaca aggtgttcgt aatctcgtgt
     8641 tgactggagt gcatttcaaa gcaaatgatt tgaaaaaagg agaccacttt atgattatgg
     8701 ctgatcatgg tactgattgg catatttacg ataacaaatt tactatggtt cataagcgta
     8761 atagtcatat ttttgattta ggatctctac aaaattcatt gtttgagaaa aaccaattta
     8821 ttggctatgc gccagaatta gtacaagacc aacagctgct atcaaaggct caagggcatg
     8881 attttttttc agaagtcatt cagtttgatg ctgctgttca tcattttgca tgggatggag
     8941 gtctacttag taatattgct ccaaactatg aagcatttaa ccaaactcga catctatgtc
     9001 acaatattac tgtaagccaa aatcaatttt taccttatat agatccgact ggttgcctga
     9061 gagcctatag tggttctatt ggtcagcatt cctcaaaagt aggagttatt agggttttaa
     9121 ataatgtttt tacctcatcc attgttacta aagcgaagct cactagttgg tttatggaac
     9181 ctattcattt tccaccaaat tcaccggtta ttgtcgcagg taatatcatt aattgatgcc
     9241 aaatacctca atatttacaa ttcaattttc atcaaaaata tgataaaata gatgaataac
     9301 cgacttgagg agtgtggcat gttaccagct tatataaaaa ttcatgatgc aattaaaaaa
     9361 gacattgatc tgggcatctg gccaattggt agtcgcttac caagtgaacg acatttagcg
     9421 gaacatttca ccgttagtcg aatgactcta cggcaggcta ttacattact agtagaagaa
     9481 gggattcttg aaaggcggat aggaagtggt acttatgttg ccagtcatcg tgttcaagaa
     9541 aaaatgcgag gaacaacaag tttcacagaa attatacgct cccaaggacg acaaccctcc
     9601 tctaaattat tatcctatca aaagcaatta gctagtgata ccgaagttaa agaattgaac
     9661 ttagacaaaa cagacttggt cattcgaatg gaacgtatcc gctatgcaga cagtgtccct
     9721 ttggtgtacg aaattgcttc aatcccagaa aaatttataa aaacggttaa acgagctgat
     9781 attacagaac acttttttca ctcattgata gcaaatggtt acgagattgg taaaagtaaa
     9841 cagacaatct acgctaagct agcaagtgag cgtgtagcct cctacttgga agttgctaaa
     9901 ggacatgcca ttttggctct aactcaggtg tcttacttta cagacggcaa accttttgag
     9961 tatgtccgca gtcagtatat cggtgaccgt tttgaatttt acttagaaaa taattagtaa
    10021 agaagtcatt tatgacttcc ttttttacac gaacagcaca aggcaattaa tttgctactg
    10081 ttaaaaacaa tcatagtatg ataaaatgaa cattatggaa attgaaaaaa caaacaggat
    10141 gaatgccctt tttgaatttt atgcagcttt gctaacagat aaacaaatga attatattga
    10201 actgtattat gccgatgatt atagcttagc tgagattgct gatgaatttg gtgtcagtcg
    10261 tcaggctgtc tatgataata ttaagcgtac ggaaaaaatt ttagagactt atgagatgaa
    10321 acttcatatg tattcggatt atgttgttcg aagtgaaatc tttgacgaca tgattgctca
    10381 ctaccctcat gatgagtatt tgcaagaaaa aatttccatt ctaaccagta ttgacaatag
    10441 agagtaagag gagttttgac tatggctttt gaaagcttaa cccaacgttt gcaagatgtg
    10501 tttaaacaca ttcgcggtaa aaagaaatta tcagaatcag atgttcaaga agtgacaaaa
    10561 gagattcgtc tagctctttt agaagccgat gtcgctttac cagttgttaa aacctttatt
    10621 aagcgcgtac gtgagcgcgc tattggccat gagattattg atactcttga tccaacacaa
    10681 caaattctta agattgttaa tgaagaatta acgagtattt taggctcaga aactgcagag
    10741 attgataaat ctccaaaaat tccaactatt atcatgatgg tcggtttgca aggggctggt
    10801 aaaacaactt ttgcaggaaa actcgctaat aaattaatta aagaagaaaa cgcgcgacca
    10861 ctgatgattg ctgccgatat ttaccgtcca gcggccattg atcagttaaa aacactgggg
    10921 caacaaatca atgtacctgt ttttgatatg gggacagacc actcagcagt cgatattgtc
    10981 agaaaaggtt tggaacaagc tcgtgaaaac cacaatgact atgtcttgat tgatactgcg
    11041 ggtcgcttgc aaattgatga aaaactgatg ggagagctgc gcgatgtcaa ggctttagca
    11101 caaccaaatg aaattctttt ggttgtagat agcatgattg gtcaagaagc tgccaatgta
    11161 gcctacgagt ttaatcatca gttgagcatc acaggggttg tgttaacaaa gattgatggt
    11221 gatacacgtg gaggtgcggc gctatctgtc cgtgaaatca ctgggaaacc aattaaattt
    11281 acaggtattg gtgaaaaaat cacagatatt gaaacatttc acccagaccg catgtccagt
    11341 cggattttgg gaatggggga tttgcttacc ctcattgaaa aagctagcca ggaatatgat
    11401 gagaaaaaat ccttagagtt agctgaaaaa atgcgtgaaa acacctttga tttcaatgat
    11461 tttattgaac agttagatca agtgcaaaat atggggccta tggaagacct cttgaaaatg
    11521 attccaggaa tggctggtaa cccagctttg gccaatatca aagttgacga aaaccaaatt
    11581 gctcgcaaac gtgctattgt ctcttcaatg acacccgctg aacgtgaaaa tcctgactta
    11641 ctaaacccga gtcgtcgccg tcgtattgca gctggttccg ggaacagctt tgtagaagtt
    11701 aataaattta tcaaagactt taaccaagcc aaaagcatga tgcaaggggt tatgtcgggc
    11761 gatatgtcta aaatgatgaa agatatgggt attaatccca ataatcttcc taaaaacatg
    11821 cctgctggta tgccagatat gtcttcctta gaaggaatga tgggacaagg tggtatgcct
    11881 gatttatctg gccttggtgg tgatatggat atgagtcaac tatttggtaa agggtttaaa
    11941 ggaaaaattg gccaatttgc catgaaacag gccatgaaac ggcaggctaa caaactaaaa
    12001 aaagcaaaga aaaaacgaaa ataaaagaga cgaaaatttt cgtctctttt tgatgtgaaa
    12061 ttcatttatc ttgtatacaa gattattaaa attacggaag accgtgttac ataccatgat
    12121 gagttcatta tcaatctaac ttaaatttct aagggcctaa aattagcgat aattttgcca
    12181 ataatgcgag gttcttcgct atagggagca aacttatcag aatatttttt atttaaggac
    12241 actagacgta atccctcatc ttcacgaaat acctttttga tatatgtttg cccatcccat
    12301 tcgactgcat aaattgctcc atcgtaatca aaactatttt gttttataag aacaacttca
    12361 ccatttaaat aagttggctc catagaatct ccaaaaaccc aagacgcaaa atcgtattct
    12421 aattgttcat cgtaaaagac aacatcaaag ttaccatcac caaaatagga gtaaccagta
    12481 ccagcagata aactttcata gacccgataa gcatataatt gtgatggttt atctatgaga
    12541 tctttagatt ttttgtctat ttgatgattt aacaatcgct ctgaatagcc aattactttt
    12601 tctttattag atattgtcag ctggttataa ggcgttagca atctatagtt tgggtcgaaa
    12661 taatcagatg tcacattgaa tagatgcaat aaggcattta aatgtttttc atttggtata
    12721 ttcttacctt tttcccaatt agatattgtc attttattga catgcaacaa tcttcccaat
    12781 tctgattgag ttattccctt tgataatcgt gctgttttta gttgatgtcc agaaaacatt
    12841 tggtagctcc tttagtaaag ttataacttt actaatagta tcaaaaaaat aaaacgaaag
    12901 caagtgtaac attttctaca attaattcat gtgatagtaa cactgtcaaa aagggggaac
    12961 acctaaaaca tagcgactag attcgtgtac tagcaggtct atatctgata aagagttaat
    13021 tactttaaag ataaaattat catttagttc tgagctaaac aaattctgtg cctgaagtta
    13081 ctaaaataat gatagtaaat tattactgaa tctaaaagaa gtgatgttag tatctgtaaa
    13141 aaagagatct gtctcgtaac atctttccga aaaactgtaa ttttcttg
//
LOCUS       DAXWIQ010000045        13007 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000045 DAXWIQ010000000
VERSION     DAXWIQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13007
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.045"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            458..1966
                     /locus_tag="ACGEUI_001426"
     CDS             458..1966
                     /locus_tag="ACGEUI_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8166255.1"
                     /translation="MLDYYLESDIIDQKILLAILLTEKELTMAAACSQTTLTALKIKQ
                     YLRQFNALFKGYLRIELIKSRIYCEVLNDKREAFFYDIFALSDTLKMLTFLLLDNPKH
                     KSIAVYTRKQGISQSKAYRLIHKLKHYLQDIGLNIVDNTVIGDELKIRYLIALLHKEY
                     GIILYDIQPADIETIHAFIFAAQKNLQPSAFLDRRFLFFDVLLMLTWKRHRYPVHLPH
                     LALFEHLKSLPIFDNIKTIAVDELAPRTRVTFSSDDFDYLFLIYLTTDNSFLSGYWTS
                     HQRQQLYHLITKDPDYHLLIHRLQALVGKYSDIHEHIPNLIPFFKRTLYNLQTLITFD
                     GYYFDQYQGNMLLLDKLETVIKDWLYDTGRQGSISSGHLHLMCLYLEQILESSIAPIN
                     ITVIESQETVGNVIANFITSTIPSYKVKLSRVNILSDNIYPYDKPVDLVVTSQKLLPF
                     LKELGVFPKETRLFGLSLDCIQQQREDLIKTILALHQNHYQKRLEELWRVPS"
     gene            complement(2315..3664)
                     /locus_tag="ACGEUI_001427"
     CDS             complement(2315..3664)
                     /locus_tag="ACGEUI_001427"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFF8166256.1"
                     /translation="MSHITFDYSKVLESFAGQHEIDFLQGQVTEADKLLREGTGPGSD
                     FLGWLDLPENYDKDEFARILTAAEKIKADSEVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTAKERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVIRVDN
                     PRKNVIIPELAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPAQDEF
                     TLGYTIYFFELAIAVSGYMNAVNPFDQPGVEAYKRNMFALLGKPGFEALSAELNARL"
     gene            complement(4120..4824)
                     /gene="speG"
                     /locus_tag="ACGEUI_001428"
     CDS             complement(4120..4824)
                     /gene="speG"
                     /locus_tag="ACGEUI_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeG"
                     /protein_id="HFF8166257.1"
                     /translation="MKTNILTIIILSCVFSYGSQLAYADENLKDLKRSLRFAYNITPC
                     DYENVEIAFVTTNSIHINTKQKRSECILYVDSIVSLGITDQFIKGDKVDVFGLPYNFS
                     PPYVDNIYGGIVKHSNQGNKSLQFVGILNQDGKETYLPSEAVRIKKKQFTLQEFDFKI
                     RKFLMEKYNIYDSESRYTSGSLFLATKDSKHYEVDLFNKDDKLLSRDSFFKRYKDNKI
                     FNSEEISHFDIYLKTH"
     gene            complement(4926..5024)
                     /gene="ffs"
                     /locus_tag="ACGEUI_001429"
     ncRNA           complement(4926..5024)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACGEUI_001429"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5224..6456
                     /locus_tag="ACGEUI_001430"
     CDS             5224..6456
                     /locus_tag="ACGEUI_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFF8166258.1"
                     /translation="MGVMMKQKIKILTVIGLMTVGMSACHNTSKPSNTDSVFSLTGKK
                     RQQIVKQVRQRYYFQQLSKTEQENYLTLYDSLAQFREIISLTPASKKSLIKTIDAFVM
                     DNPEFYWITSADYRFEFSDQTVFVTFPIPEDAKNIYQDLQAIGNDIVANTPSKDRYEQ
                     VKYFYEVIIRDTDYNKKAFEAYQSGSQAQVASNQDIKSVFIDHLSVCNGYAQAFQFLC
                     QKAGIPVAYIRGTGTSQQPQQSFAHAWNAVQINNTYYGVDVTWGDPVFDNHLSHQKQG
                     TINYSFLCLPDYLMALSHQPSKDIAFNTKERFENVWTIPSCTDDSLLYSKRHQSYIST
                     FDSDAILASLENQLLNRQEPLSLQFAHQDDYQQMVTDLTTNQTGYHNLFNQYWNNYTG
                     FTYGLLPETLSISFASRN"
     gene            complement(7070..7585)
                     /gene="tadA"
                     /locus_tag="ACGEUI_001431"
     CDS             complement(7070..7585)
                     /gene="tadA"
                     /locus_tag="ACGEUI_001431"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFF8166259.1"
                     /translation="MPYSLEEQTYFMQEALKEAEKSLQKAEIPIGCVIVKDGEIIGRG
                     HNAREESNQAIMHAEMMAINEANAHEGNWRLLDTTLFVTIEPCVMCSGAIGLARIPHV
                     IYGASNQKFGGVDSLYQILTDERLNHRVQVERGLLAADCANIMQTFFRQGRERKKIAK
                     HLIKEQSDPFD"
     gene            complement(7585..8364)
                     /locus_tag="ACGEUI_001432"
     CDS             complement(7585..8364)
                     /locus_tag="ACGEUI_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8166260.1"
                     /translation="MKLTTLGSWGAYPYQDAGTTSYLVTGHDGFQLLMDAGSRALNEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLRHYFQLYPKHLWQPKVLPIYGHDEDRNEFA
                     KLSIPDVSEGRAYDVTGVEQIGSFDITFLKTVHPVVCYAFRIVERATGQVLVFTGDSG
                     YFEGLADFAKEADLFLADVYLYEGNENHMAHLTSKEAGLMASQAGVKKLVLTHMPPVP
                     PDGINSKNHLEVLRQETQAYAGNIPVDLALPHKSWNLGSLS"
     gene            complement(8504..9043)
                     /locus_tag="ACGEUI_001433"
     CDS             complement(8504..9043)
                     /locus_tag="ACGEUI_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFF8166261.1"
                     /translation="MFTTKELVKVAMMTTLIIILGFIPAIPLGFIPVPIVLQNLGVML
                     AGLMLGGKKGTLSVFLFLVIGLFLPVFSGSRTTIPVLMGPSAGYVIAYLLVPIVFSLL
                     YRNWFSKSTPLAFLALLISGVVLVDVLGAIWLSAYTGMSLVTSLLSNLVFIPGDTIKA
                     IIATIIAVKYKDSFLNTKQ"
     gene            complement(9047..9358)
                     /locus_tag="ACGEUI_001434"
     CDS             complement(9047..9358)
                     /locus_tag="ACGEUI_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHY zinc finger protein"
                     /protein_id="HFF8166262.1"
                     /translation="MTDCFGIDLDQEYRCLHYHTPLDIVGLKCASCQTYYACYHCHDQ
                     LTDHAFVPTGHQETSPVICGHCRKLLSRAEYGCGCCPYCQSPFNPACHRHKDIYFLKE
                     S"
     gene            complement(9575..10717)
                     /gene="tgt"
                     /locus_tag="ACGEUI_001435"
     CDS             complement(9575..10717)
                     /gene="tgt"
                     /locus_tag="ACGEUI_001435"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFF8166263.1"
                     /translation="MTDYPIKYRLIKAEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKAIGSGIILSNTYHLWLRPGDELIARSGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSI
                     GGLAVGESHEEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSEGRLVIKNAKFAEDFTPLDHDCDCYTCQNYSRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKKVRQAIMDDNLLEFRQDFLERYGYNKSNRNF"
     gene            10936..11799
                     /locus_tag="ACGEUI_001436"
     CDS             10936..11799
                     /locus_tag="ACGEUI_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFF8166264.1"
                     /translation="MSIKAIKGQARDTLKNLSGKYLLFLIPTLLFMFHFGIEIHQGYV
                     LSSGIEVSLAASYFPLLLGLILSLFILSASFTMIDVVRHFRQKVSFAESTTAFSKEFF
                     GNLLVLAITKWLFFLIWSLIWFFGLFIFLSGLSAFLVNAKSGSSTVISLIFLLFGAVL
                     SLIGFGIYINRYYAYSLSEYLLYDEVKEGTYLGAIAVIETSVAMMKGYKWKLFFLQLS
                     FTGWFLLNIVTFGLLNIYLLPYFTTANVIFYDQLKKRFKDKDDPIEGEHLSLHQLNKN
                     PTPMMYDDQKD"
     gene            11885..13007
                     /locus_tag="ACGEUI_001437"
                     /pseudo
     CDS             11885..13007
                     /locus_tag="ACGEUI_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         4060 a         2602 c         2311 g         4034 t
ORIGIN      
        1 aactagctct ctcaaaaaag attaaaaata aaggcattct aaagaatcat ttaagaaaac
       61 tttaaggtta ctatcgtaga atgaaaacat cctaaaaagc ggtttaccta aaccgttggg
      121 agaataggtt ttccccttgt tttccctttt cataaatctc ctgggcacct atcttcttag
      181 gtgccctccc ttgtttaaac ctgaccagtt aaccggctgg ttaggttttt aaatttaaaa
      241 agtcaacagc aaaaatatga tttcacccgc gtgttcttag ctctctcaat tatgggctaa
      301 aaacacgcat caatcaatct attgatgaag tttgctgctt tttgatgatc tttattgtga
      361 ctatactggt aatcctattg attttagttg acttttacgg tagattcgct acaatatata
      421 ggattataca atttaattta aggagagaaa aattacattg ttagattatt atttggaatc
      481 cgatattatt gaccagaaaa tcctactggc tattttgttg acagaaaaag aactgacgat
      541 ggcagcagct tgttctcaaa caactttgac tgctttaaaa ataaagcagt atcttcgaca
      601 atttaacgcc ttatttaaag ggtacttacg tattgagctt attaaatctc gtatttattg
      661 tgaggtatta aatgataagc gagaagcatt tttttatgac atttttgcct tatcagatac
      721 tctaaaaatg ttgacattct tattactaga taatcccaaa cacaaatcaa tcgctgttta
      781 tacacgaaaa caagggatat cccagtcaaa agcttatcgt cttattcata aattaaaaca
      841 ttatttacaa gacattggtt taaatattgt agacaatact gttatcggag atgaacttaa
      901 aatacggtat ttgattgctc ttttacataa agagtacggc attatattat atgatataca
      961 acctgcagac attgaaacga ttcatgcttt tatctttgca gcacaaaaaa acttacaacc
     1021 ttctgccttt ttagatagac gcttcttgtt ttttgatgtg ctgttgatgt tgacttggaa
     1081 gcgccatcgc tatcctgttc atttgcctca tttagctctt tttgaacatc tcaaatcctt
     1141 gcctattttt gacaatatca aaaccattgc cgtggatgag ctagcacctc gaactcgtgt
     1201 cacattttcg tctgatgatt ttgactatct ctttttaatt tatttaacga ctgataactc
     1261 cttcttgagt gggtactgga caagtcatca gcgacaacaa ctttaccatt taatcaccaa
     1321 agatcccgat tatcatttac taatccatcg tcttcaggca ctagttggaa aatattccga
     1381 tattcatgag catatcccta acttaattcc ttttttcaaa agaactctct acaatttaca
     1441 aactttaatt acctttgatg gttattattt tgatcaatat caaggcaaca tgctactact
     1501 agataagctt gaaaccgtta tcaaagattg gctttatgac acaggccgtc aaggcagtat
     1561 tagttctggt cacttacact tgatgtgttt ataccttgag cagatcttgg aaagctctat
     1621 tgctccaatc aatattactg ttattgagag ccaagaaact gttggtaacg ttatagctaa
     1681 tttcatcacc tcaactatcc cttcttacaa agttaaactg tcacgggtca atatcttatc
     1741 tgataatatt tatccctatg ataaacctgt agatctggtt gtaaccagtc agaagctctt
     1801 gcctttctta aaagaactcg gtgtttttcc caaagagacc cgcttgtttg gtttgagttt
     1861 ggactgtatc caacagcagc gcgaagattt aatcaaaacc atcttagcct tgcaccagaa
     1921 ccactaccaa aaacgtttag aagaactttg gagagttcct tcttaatttt ttgacagtcc
     1981 ctcctagata ctttcacagt ctaggagggt ctttaaggtt gtcacttgca accttgataa
     2041 gctctctttt tagccattgc tatttgctta gaaacagaac tggaactgtt atagcggttc
     2101 aactcctctt gtaggatctt cttccctttt ttgaaggtct tatctgaaat ttggtataat
     2161 aaagtgcgct cccatgttct ggaagcgcac tttattatcc cttttactta accttgtatc
     2221 cactcgatga tggaagtgcc gccccaattc ttagattgct tggctaactt ttaagctctt
     2281 gtacaaggaa gaagtctttt tgactatctg ttgattagag gcgagcatta agctctgctg
     2341 ataatgcttc aaaacctggt tttccaagaa gcgcaaacat gttacgtttg taagcttcaa
     2401 cacctggttg atcaaatggg ttaacagcat tcatgtagcc tgaaacagcg atggcaagct
     2461 caaagaagta aatagtgtaa ccaagggtaa actcgtcttg agctggaaga gtgacaaaca
     2521 tgtttggcac gccgccatct gtgtgggcaa gaagcacacc atctgtagct ttcttgttaa
     2581 cgaaatcaac gtctttacct tgaaggtaac caagtccatc taggtcttct gctaattcag
     2641 gaatgataac atttttacga ggattatcaa cacgaataac tgtttcaaag agattacggt
     2701 aaccttcttg gataaattga ccaagtgagt gaaggtctgt tgagaagtta gctgaagttg
     2761 ggtaaatccc tttttgatct tttccttctg attcaccagc taattgtttc caccattcgc
     2821 caaagtactg aagagatggc tcgtagttag ccaagatttc agtgatgtag cctttacggt
     2881 aaagcacatt acgaacagca gcgtattggt aagcaatgtt ttcagagatt ttgtctgaag
     2941 aaaggtcttt acgagcagca tttgcccctt ccataagagc agtaatatct gcacctgatg
     3001 cagcaattgg aagcaaacca acagcagtca agactgagaa acgaccacca acattatctg
     3061 gcaccacaaa tgtttcccag ttgttagcgt cagcttctac cttaacagca cccttaactt
     3121 tgtctgtggt agcgtagata cgtttgttag cttcttcttg gccatatttt ttaaccaaaa
     3181 gttctttgaa cacgcgaaag gcaatagctg gttcagtggt tgtacctgat ttagagatca
     3241 cgtttactga gaactctttg tcttgaacgt attcaactaa atcggcaagg taagtagatg
     3301 aaatagagtt tccagcgtaa agaatttgtg gggctttgcg ctctttggca gtttgcaagt
     3361 tagcaaaatg atgatttaaa aaatcaatcg ctgctttggc accaaggtat gacccaccga
     3421 taccgatcac cacaagaact tcgctgtctg ccttaatctt ttcagcagca gtcaagatac
     3481 gtgcaaattc atctttatca tagttttctg gaagatcaag ccagcccaag aaatctgaac
     3541 ctggtccagt tccttcacgc aataacttgt cagcttctgt tacttggcct tgtaaaaaat
     3601 caatttcatg ttgtcccgca aatgactcga ggacttttga ataatcaaat gtaatatgtg
     3661 acatgagttt cgctccctta aaattttaag tttcattagt atgataacct tttctatcta
     3721 atatttcaat ggtttttagt ggttttcacc tgtgaaaaag ctaggttttt tgaaaaggtt
     3781 ttcgtttttt cagacataaa acatgtgtca atgcaagcat attctcatca tagtggtttt
     3841 ttaagagaaa ataggatttc atatttccta aaaatatcat gaaaaaaggc cctgaggccg
     3901 ctttaccgta ataacttatt ttaaggattt tatcatttca acacgggtaa taaccccatc
     3961 atgcatatga tcaatgcata aagccacacc tacaaaggca attgaagccc caatcacctg
     4021 taaatacttt ctcatgctat cttcttaaca ttacttactc tcattatcta aaaatggcaa
     4081 tcatttgaat acttaaaaag caaagggggg agaatagcac tagtgcgttt ttaagtagat
     4141 atcaaaatga ctaatttctt cactattaaa aatcttatta tctttatacc ttttaaagaa
     4201 actgtctcga cttaaaagct tatcatcctt attaaataaa tcaacttcat aatgtttact
     4261 atctttagta gcaaggaaaa ggctccccga tgtataacgc gattccgaat catagatatt
     4321 gtatttttcc attagaaatt ttcttatttt aaaatcaaat tcctgtaaag taaactgttt
     4381 cttttttatg cgaacagcct cagagggcaa ataagtttct ttcccatctt gatttaaaat
     4441 tcctacaaac tgtaatgatt tatttccttg attcgaatgt tttacaatac caccataaat
     4501 attatctaca taaggtgggg aaaaattata agggagacca aaaacatcga ccttatcccc
     4561 ttttataaac tgatcagtaa tgcctaaaga tacaatagaa tcaacataaa gaatacattc
     4621 cgatcttttt tgtttagtat taatatgtat gctatttgta gtaacaaatg caatttctac
     4681 attttcataa tcgcatgggg taatattata ggcaaatctt aaacttcttt ttaaatcttt
     4741 taaattttca tctgcataag ctaattgact tccatagcta aaaacacatg ataatatgat
     4801 aattgtcaaa atgtttgttt tcatttaaaa ctttttatgt cctgttttat acctcttagc
     4861 ctaaataaaa atatcaatcg gtttcaacaa ctgatgacac tatagaagga ggaaattact
     4921 ttagtaaagt caacaaaaaa agagcacaca cccgaaaatc gcttagggct gctggattcc
     4981 tcccctgacc cacttcacgc acaagtgttg ctccgcctat tattatatca tattacggac
     5041 agtttgcaag ttttccaact aagtaaaatc actttcaagt gagcatagct tcattttggg
     5101 acaagatgga ctgaatacct agaaaaatca tgaccctttc caaacacgat tttgcttttt
     5161 gaaaatcttt ccctctcccc attccagcta ttctctatgt tataataaga ttattagatt
     5221 agaatgggag ttatgatgaa acaaaagatt aaaatcttaa cagtaattgg ccttatgaca
     5281 gttggtatgt ctgcgtgtca caacactagc aaaccttcta ataccgattc tgttttttct
     5341 ttaaccggaa aaaagcgcca acaaatcgtc aaacaagtaa gacaacggta ttatttccaa
     5401 caattatcca aaaccgaaca agaaaattac ctcactttat acgatagttt agcacaattt
     5461 cgtgaaatca tcagtttgac gccggcttct aaaaaaagct taattaaaac aattgacgcc
     5521 tttgtgatgg ataatcccga attttactgg attacatctg ccgattatcg atttgagttt
     5581 tctgatcaaa cggtctttgt cacctttcct atcccagaag atgccaagaa catttatcaa
     5641 gacttgcaag caatcggtaa tgatatcgtc gcaaacacgc catcaaagga tcgctatgag
     5701 caggtcaaat atttttatga ggtcatcatt cgagacacag actacaataa aaaagccttt
     5761 gaagcctatc aatcaggcag tcaagcccag gtcgcttcta atcaagacat taaaagtgtt
     5821 tttattgatc atttatctgt ttgtaatggc tatgcccaag cctttcagtt tctttgtcaa
     5881 aaagctggca ttccagtcgc ctatatccgt ggaactggta catctcagca acctcagcaa
     5941 tcttttgcac atgcatggaa tgctgttcaa attaataaca cttattatgg cgttgatgtc
     6001 acttggggtg accctgtttt tgataaccat ctctcacatc aaaagcaagg aactatcaat
     6061 tacagttttc tatgcctgcc agattatcta atggccttat cacatcagcc aagcaaagac
     6121 attgctttta atactaaaga acgttttgaa aatgtttgga ctataccttc ttgtacagat
     6181 gattctttgc tttattctaa acgtcatcaa agctatatct caacgtttga cagtgacgcc
     6241 atccttgcaa gccttgaaaa tcagttgtta aatagacaag agccactgtc tctacaattt
     6301 gctcatcaag atgattatca acaaatggta actgatttaa cgacgaatca aacaggttat
     6361 cataaccttt tcaatcagta ttggaataat tatacgggct ttacctatgg actcttacca
     6421 gagactctta gtatttcatt tgctagtagg aactaggttt agacacatga aacacaaagt
     6481 taagatgatt atctttgtca aacattacaa gaaattgata tactaacaca attgaaggcg
     6541 attattattc taaccgtctt caatagatat aaccttttca gattgagcac atctaatgct
     6601 tgtaacatta gcaacagtga tgagctcttt tttaatgctt tagcaaaata tatcagctaa
     6661 gacaaaagat taaataactc aagccatgcc tataagtcac ttgtttttaa tccctgtgtc
     6721 agtgtcaatc tacgcaacag ataactgaca gctctgagcc ctagaagtct gaacttgata
     6781 agtttactaa aaacaaaaat aaggtgagcc attactatct gtttttagat agtaaatctt
     6841 tttaagaatg actaactcat ctagtcatca cgtgttttat ttttcgattg ttttcatctc
     6901 gattgggtct gaaaaagagc gcttctagta cttggaaacg cccttttgta tattctatct
     6961 tcgtatgttg aatctaacaa ccattggaaa aatggtctaa cgtttcactc agtattttga
     7021 caggctaccc ttcaccaaaa atgcatgtga taacaaactc cttaaaactc tagtcaaagg
     7081 gatctgactg ttccttgatc aaatgcttag ctattttttt gcgctctcgc ccttggcgga
     7141 aaaaggtttg catgatgtta gcacagtctg ctgctagcag gcctcgttca acttgcacgc
     7201 gatggtttaa gcgctcatct gtcaaaatct ggtaaaggct gtctactcca ccaaattttt
     7261 gattgctggc tccataaata acatgcggaa tccttgctag cccaatggca ccactacaca
     7321 tcacacaagg ctcgatggtc acaaagagtg tggtatctaa taaacgccag ttgccttcat
     7381 gagcgttagc ctcgttaata gccatcattt cagcgtgcat aatggcttgg ttactctctt
     7441 cacgcgcatt gtgcccacga ccaataatct caccatcctt gacaatgaca cagccaatgg
     7501 ggatttcagc tttctgtaag gatttttcgg cttcttttaa agcctcttgc atgaaataag
     7561 tttgctcttc taaactatat ggcatcaaga aagactcccc aagttccaag atttgtgtgg
     7621 taaggcaagg tcaacaggaa tattacctgc ataggcctgt gtttcttgcc tcaacacttc
     7681 taaatggttt ttggaattaa taccatctgg aggaaccggt ggcatgtggg tcaagacaag
     7741 cttcttaaca cctgcctggc tagccattag tcctgcttcc ttactggtca aatgggccat
     7801 atgattttca ttgccttcgt ataagtaaac atccgctaag aaaaggtctg cttcttttgc
     7861 gaagtctgcc aatccttcaa aataaccact gtctccagta aagacaagta cctgaccagt
     7921 cgctctttct acaattcgaa aggcataaca cacaactgga tgaactgttt taagaaaggt
     7981 aatgtcaaaa gaaccaatct gttcaactcc agtaacatca taggctctac cttctgaaac
     8041 atcaggaatg cttaacttag caaattcgtt tcggtcctca tcatgcccat aaatgggcaa
     8101 tactttaggc tgccacaggt gtttaggata aagttgaaaa taatggcgca acacgcctaa
     8161 atcggccacg tggtctggat ggtaatggct aataatcact gcatccaaat cgagaggact
     8221 gatttctttt tccagctcat tgagcgctcg actaccagca tccatcaata actgaaagcc
     8281 gtcatgtcct gtgaccaaat aagaggttgt gcctgcatct tgatagggat aggctcccca
     8341 agaccctaag gtggttaatt tcatctcaaa cccttctttc ttgctttaac tcttattata
     8401 acaaaaatta gtgtcaaaga ggattgagac ccaccaaaaa caggtcctag cagataaatc
     8461 tgccaagacc tgttcaacac aacttaacta tttgatgatt agattactgt tttgtattca
     8521 aaaaactatc tttgtattta acagcgataa tggtcgcaat gattgctttg atggtatctc
     8581 ctggaataaa gaccaagttt gataaaagcg atgtcacaag agacatgcca gtgtaggctg
     8641 acaaccagat agcacccaga acatcaacca gtacaactcc tgagattaaa agagctagga
     8701 atgctaatgg cgtgcttttt gagaaccaat tgcggtaaag taatgaaaag acaataggca
     8761 caagaaggta ggcaataaca tagccagcag atggccccat caatactgga atggttgttc
     8821 ttgagcctga gaaaacaggt aagaaaaggc caatcactaa aaataagaaa acagataagg
     8881 ttcccttttt accacccagc atcaatccag ccaacatgac acctaagttt tgtaaaacaa
     8941 tagggactgg gataaagcct agcgggatag caggaataaa gcctaaaata ataatcaaag
     9001 tggtcatcat agccacctta acaagttctt tggttgtaaa catcagttac gactccttta
     9061 aaaaatagat atccttgtgc ctatgacagg ctggattaaa gggactttga caataaggac
     9121 agcatccaca accatactca gctcgactca agagctttcg acaatgccca caaataacag
     9181 gagatgtttc ttgatgtcct gttggcacaa aggcatgatc tgttaactgg tcatggcaat
     9241 ggtaacaagc ataataagtc tgacaagaag cacatttgag cccaacaata tcaagcgggg
     9301 tatgataatg taggcaccga tactcttggt caaggtcaat tccaaaacag tccgtcatat
     9361 ctcatcctct tgtaagagtt taatcaaaag cttatgattt gtcaactcta tttttacaaa
     9421 agggtaactg ttttctgaaa aagaacacgt aaaacaagaa cttcccttta aagagtgacc
     9481 tatcagttga aagcaatagg acatccgtct atacaaaaaa actgaaacca aagacgatag
     9541 atcgccctca gttccaggat catagcattt ttaattagaa attacggttt gatttattat
     9601 aaccataccg ttcaagaaaa tcttggcgga attcaagtaa gttatcgtcc ataatagcct
     9661 gacgtacttt tttcatcaag ttaaccaaga aataaaggtt gtggtagctt gtcaaacgaa
     9721 taccaaaagt ttcgtccgct tttagcaagt gacgaatgta ggcacggcta tagttttgac
     9781 aagtgtagca gtcacagtca tgatcaagtg gggtaaaatc ttcagcgaat ttggcatttt
     9841 taatcacaag acgcccctca ctagtcatac aagttccgtt acgggcgata cgcgttggca
     9901 agacacagtc aaacatatcg acaccacgaa tcacaccatc aatcaagcta tctggtgctc
     9961 ccactcccat caggtaacga ggtttatttt ctggtaagag gggagtcgta aagtcaagga
    10021 cggcattcat ttcttcatgg ctctccccaa cagctaaacc accaatagaa taacctggga
    10081 aatccatagc caccaaatct gccgcagact gacgacgaag gtcctcaaat ccagctcctt
    10141 gcacaattcc aaataatcct tggtcatgag gacgacgatg ggctttcaag ccacgttctg
    10201 cccaacggct agtacgttca atggattttt tcacgtaatc ataaggttgg taaaattgcg
    10261 ggcactcgtc aaaactcatc atgatgtctg agccaaggtt attctgaatg gaaatggctt
    10321 tttcgggtga tagaaacatt tttgagccat tcaagtgatt tttgaaagtc actccttctt
    10381 cagtgatgtt tcgtgaatcg gctaaagagt aaacttgaaa accacccgaa tcggttaaga
    10441 ttggctgatc ccagttcata aacttgtgca gaccaccaga gcgagcaatc aactcatctc
    10501 ctggacgcaa ccagaggtga taagtgtttg ataaaataat ccctgatcca attgctttta
    10561 actcttctgg agactgtgtt ttaacagtag cctgagttcc cactggcata aacattggtg
    10621 tcggaaaggt cccgtgaggg gtgataattt cgcccaagcg tgcaccggtg tgcttttctg
    10681 ctttgataag gcgatactta atcggataat ctgtcatgtg ctttcttcct tgctatcaag
    10741 ttaaacagtc ttgagctttt ttgatctgcc tgagtttcca tcataatgaa gggcttcaag
    10801 atgacatgtt gttctagcgc ctatcatttt atcaaaaaat ccataatttg tcatgctttc
    10861 ccaaaaaagt atagtcaaaa taatggttag ctgtctcatt ttgttttatg gtataatgaa
    10921 ataaaggagt ctgttatgtc aattaaagca atcaaaggcc aggctagaga cacgttaaaa
    10981 aatctctctg gcaagtattt actattttta attccaaccc ttctctttat gtttcatttc
    11041 ggtattgaga ttcatcaagg ttacgtccta tctagtggta ttgaagtctc gctagcggct
    11101 agttatttcc cattactact aggacttatc cttagccttt tcattttatc agcatccttt
    11161 acgatgattg atgttgttag gcattttcgt cagaaagttt cttttgccga atcaacaaca
    11221 gccttctcaa aagaattctt tgggaacctg ctggttttag cgatcactaa gtggctattc
    11281 tttctcatct ggtcactaat ttggtttttt ggcctcttta ttttcctttc cggactttca
    11341 gcgtttttgg tcaatgctaa atcaggtagc tcaacagtca tttcactgat ctttttacta
    11401 tttggagcag ttttgtcctt aattggtttt ggtatttata tcaaccgtta ttacgcttat
    11461 agtctatcag aataccttct ctacgatgag gttaaagagg ggacttatct aggagctata
    11521 gccgtcattg aaacaagtgt agctatgatg aaaggttata aatggaagct ttttttccta
    11581 caacttagct tcaccggttg gtttctactg aatattgtta cttttgggtt acttaatatc
    11641 tatcttttac catattttac aacagccaat gtcatttttt atgatcaact caagaaacgt
    11701 ttcaaagaca aagatgaccc aattgaagga gaacatttaa gccttcatca gcttaataaa
    11761 aatcctactc ctatgatgta tgacgatcaa aaagactaaa taaatcctcc atccataaaa
    11821 atagcccttg aactgcaccc caaaagttag acacaaaatc taacgattgg ggtgctattt
    11881 ttttatgaaa tttaatcaag aaacgaaagt taagatttat gagttacggc aaatgggaga
    11941 gtccattaaa tccatatcaa aaaagtttga tatggcagaa tccgatctca aatatatgat
    12001 tcgcttgatt gataggtatg gggtaaccat tgttcaaaaa tgtaagaatc attattattc
    12061 tccagaactg aagcaagaga taataaataa agtcctgatt gacggccaat ctcagaaaca
    12121 aacatcctta gattatgctt taccaacttc tagtatgctc tcaaggtgga tagcgcaata
    12181 caagaaaaac ggttatacta ttcttgaaaa accaagaggg agaccgagta agatggggcg
    12241 taaacgtaag aaaaatttgg aagagatgac agaggttgaa cgtcttcaga aggaattaga
    12301 ataccctaga gcggagaatg ctgtgctaaa aaagctgaga gaataccgct tgagggacga
    12361 agtaaagctc aaagagcaac agaaatcatt caagcattaa ggaatcaatt tcccttagag
    12421 atgctacttg aaatccttga tttatcacgc tccacctatt actatcaagt gaagcgacta
    12481 gctcaaggag ataaggatat agaactaaag catgtgattc gagagattta tgatgagcat
    12541 aaaggtaatt acggttatcg tcggattcat atggagttgc gtaaccgagg atttgtggtc
    12601 aatcataaga aagttcaacg tctcatgaaa gtcatgggct tagcagcccg cattcgtcgg
    12661 aagcgcaagt attcttctta caaaggagag gttggtaaga aggctgataa tctgattaaa
    12721 cgtcacttcg aaggctctaa accttacgaa aaatgttaca ctgatgtgac ggagttggcc
    12781 ttacctgaag gaaaactcta cttatcacct gttcttgatg gctataacag tgagattatt
    12841 gatttcactc tatctcggtc tcctaacttg aaacaggttc aaaccatgct tgagaagact
    12901 tttccagcag attcatacag cggaactatt cttcatagtg accaggggtg gcaataccaa
    12961 caccagtcct accatgattt tttagagtct aaaggtattc tggcatc
//
LOCUS       DAXWIQ010000046        12084 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000046 DAXWIQ010000000
VERSION     DAXWIQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12084
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.046"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            148..2346
                     /gene="nrdD"
                     /locus_tag="ACGEUI_001438"
     CDS             148..2346
                     /gene="nrdD"
                     /locus_tag="ACGEUI_001438"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFF8166265.1"
                     /translation="MVSLEEDKVTVQPDIKVIKRDGRLVNFDSTKIYSALLKASMKVT
                     RMSPLVEAKLEAISDRIIAEIIERFPTNIKIYEIQNIVEHKLLAANEYAIAKEYINYR
                     TQRDFARSQATDINFSIDKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAELNFKKHMADAKKWIVETKRESYAF
                     EKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEHEIQKAILTIRINGL
                     GSEHRTAIFPKLIFTVKRGLNLEPDSPNYDIKTLALECATKRMYPDMLSYDKIIDLTG
                     SFKSPMGCRSFLQGWKDENGQDVTSGRMNLGVVTLNLPRIAMESNGDMDKFWELFNER
                     MLISKDALIYRVERVTEAKPANAPILYQYGAFGKRLEKTGNVNDLFKNRRATVSLGYI
                     GLYEVASVFYGGQWEGNPDAKAFTLSIVKAMKQACEDWSDEYGYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKSPTPFEKLDFEKDYPEAGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYNCQFEGDFTPTERGFTCPNCG
                     NNDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSTIKYPGL"
     gene            2528..2674
                     /locus_tag="ACGEUI_001439"
     CDS             2528..2674
                     /locus_tag="ACGEUI_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166266.1"
                     /translation="MGKYQLDYKGMQQVERFHEKHSKKKTDKKSRVQELKARFLEKSK
                     KQDN"
     gene            2703..3638
                     /locus_tag="ACGEUI_001440"
     CDS             2703..3638
                     /locus_tag="ACGEUI_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8166267.1"
                     /translation="MLNIGIVGLGAISQKAYLPYMRQLSDITWHLSTRNAAVRQQVGQ
                     LFGHAILYSDVKELSKTNLDGVFIHAATSAHAELASLFLNQGIPVFMDKPIADNYLMT
                     KNLYDLAKENQTFLMAGFNRRFTPRVKKLSSLSTKRKVAVEKNDLNRPGDMTFKLFDF
                     FIHPLDTALFLTEGTLLKGHFQYHLEAGLLSQVMVTLMTESMTTTASMNLQSGSRREV
                     MEVQRAEETYHLENLDELSIYKGTEKRVLGFASWDTTLHKRGFETMIDAFLEAISTGV
                     NPVSPESSLLSHWICQQIADSQLSYGELTVELPKD"
     gene            3647..4156
                     /locus_tag="ACGEUI_001441"
     CDS             3647..4156
                     /locus_tag="ACGEUI_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8166268.1"
                     /translation="MEIRRPTLKDKDAVLSMINEFLEQKSATDGLWHFNVNDFNYETW
                     LEDSLRQEMGLSSQGVPAIQYVAFDERSQAIGFLNLRLRLNERLLEKGGHIGYSVRPS
                     QRGKGYAKEMLKQAVSYAISKNITTILVTCDETNIASRAVIVANGGILEDSRGGTERY
                     WIEEQRGDK"
     gene            4156..4770
                     /gene="nrdG"
                     /locus_tag="ACGEUI_001442"
     CDS             4156..4770
                     /gene="nrdG"
                     /locus_tag="ACGEUI_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFF8166269.1"
                     /translation="MAEKCWNNPKPKEWQAEELSQGRIIDYKAFNFVDGEGVRNSLYV
                     SGCLFHCKGCYNAATWSFKAGMPYTQELEEQIMTDLAQPYVQGLTLLGGEPFLNTGIL
                     IPLIKRIRRELPEKDIWSWTGYTWEEMMLETPDKLEMLSLIDILVDGRFDITKKNLML
                     QFRGSSNQRIIDVQKSLAAKEVIIWDKLNDGDQTFEQISREDLL"
     gene            complement(4944..5675)
                     /gene="yaaA"
                     /locus_tag="ACGEUI_001443"
     CDS             complement(4944..5675)
                     /gene="yaaA"
                     /locus_tag="ACGEUI_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFF8166270.1"
                     /translation="MLTFLIPTAKEMTIPKESHPHLLPQDSQAILKIMAAMTTEDLAK
                     SYRIKEESAKKEQQRWQDMASQQSLAYPAYQLFNGLMYRHIKRDKLTTQEQAYLTQQV
                     YITSSFYGIIPANHPIAEHRHDFHTRIKIEGQSLKSYWRPCYNQFAKEHPQVISLLSS
                     EFDDVFSKDCKQLWISPKFMAEKEGQFKTHSTISKKARGAFLTACMENNCQTVDSLKS
                     LVFAGFYYHPDLSTDHEFVYIKKEA"
     gene            complement(5717..6574)
                     /locus_tag="ACGEUI_001444"
     CDS             complement(5717..6574)
                     /locus_tag="ACGEUI_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8166271.1"
                     /translation="MIYPYNSTISLGTVSLNVTDLAKMTTFYTSIIGLQVLSQDTTSR
                     QLTTDGKTVILELRQTPLPGDKAYGLYHTAFLVPDRHSLGLVLNHFLTRSISLEGAAD
                     HGHSEAIYLSDPEGNGIEIYHDKAVEHWDIRDNGQIIGVTEPTDTKSILEQLTDIPKH
                     FLLAQDTRIRHVHLSVKNALASSLLYQKVFDLGDKMTIPSASWIASGNYYHHLAFNHW
                     SAPYLKKHQEGAPGLAFLTIHIETPLLFSATLKKARLHGLAILQEDSSSFTTEDEEGI
                     RVNVILGQD"
     gene            6833..7099
                     /locus_tag="ACGEUI_001445"
     CDS             6833..7099
                     /locus_tag="ACGEUI_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFF8166272.1"
                     /translation="MLRDLLKGTLRFGQLKSSIGSVSQKVLTAQLRAMEADGLVHREV
                     YAEVPPRVEYSLTETGLSLAPVIEAMSDWGQTYQEKHADLFEEG"
     gene            complement(7378..8091)
                     /gene="treR"
                     /locus_tag="ACGEUI_001446"
     CDS             complement(7378..8091)
                     /gene="treR"
                     /locus_tag="ACGEUI_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFF8166273.1"
                     /translation="MTKYERIYKDLETKINKDFYKEGDFLPTEIELSQQYQASRDTVR
                     KALSLLTKAGLILKKQGRGTQVIRHHQIMFPISELTSYQELVSYSNLDSKTNVIAIDK
                     LIVDETLSKLTGFSKNSLVWRVTRQRVVEGVASVLDIDYLSKTLVPIMTREIAEHSIY
                     QYLEKELHLAIDFALKEVTIDQITDRDKILLDLGSDQHVVSVKSKVYLSNNNQFQFTE
                     SRHKLEKFKFLDFARRRPK"
     gene            8302..10326
                     /gene="treP"
                     /locus_tag="ACGEUI_001447"
     CDS             8302..10326
                     /gene="treP"
                     /locus_tag="ACGEUI_001447"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFF8166274.1"
                     /translation="MGKFEQDAKSLLTAIGGKENIKVVTHCATRMRFVLNDNNKANVK
                     EIEKISVVKGTFTNAGQFQVIIGNDVPVFYNDFTAVSSIEGVSKEAAKSAAKSNQNAL
                     QRVMTMLAEIFTPIIPAIIVGGLILGFRNILESVPFEFLGQQVEKGKLVFDAAGDPVW
                     NTIVRVSPFWSGVNHFLWLPGEAIFHFLPVGITWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYAVAGTPAAEIAKNWVWDFGFFTINRIGYQAQVIPALLAGLSLAYLEIFWRKRI
                     PEVVSMIFVPFLSLIPALILAHTVLGPIGWTIGKGISFVVLAGLTGPVKWLFGAIFGA
                     LYAPLVITGLHHMTNAIDTQLIADTATRTTGLWPMIALSNIAQGSAVFAYYLMNRHEE
                     REAEISLPAAISAYLGVTEPALFGVNVKYVYPFVAGMIGSGIAGLLSTTFNVQANSIG
                     VGGLPGFMAINVKYMIPFFICMAVAIVVPMFLTFFFRKSHIMTKTEDEAKLPETPVSD
                     APVATAPHKTMQGTVITLTSPLTGEVKALSEAVDPVFAQGVMGQGALLQPTEGVLVAP
                     CDAEVSVLFPTKHAICLVTTEGLELLMHIGMDTVNLDGQGFEALVKQGDQVKAGQTLI
                     QFDIAAISEAGYATETPLVVTNQDVFTVTVEGSLPRQIKVNDKLAVAVKK"
     gene            10393..12021
                     /gene="treC"
                     /locus_tag="ACGEUI_001448"
     CDS             10393..12021
                     /gene="treC"
                     /locus_tag="ACGEUI_001448"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFF8166275.1"
                     /translation="MTIDKKKVVYQIYPKSYKDTTGNGVGDLLGIIDKLPYLQELGID
                     MIWLNPFYPSPQRDNGYDVSDYTAVNPDFGTMADFENLVKAAKEHQIELMLDMVLNHC
                     STDHEWFQKALAGDPYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHVREELAKVVNFWRDKGVKGFRFDVINLIGKDEELVDCPVNDGKPAYTDRP
                     ITHTYLHDLNQASFGQDDSFMTVGEMSATTIDNCLLYTAPEREELSMAFNFHHLKVDY
                     ENGQKWTIMAFDFAALRDLFHAWGEGMSQGNGWNALFYNNHDQPRALNRFVDVTHFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDFDSMDDYVDVESLNAYSSLLVSGKSAE
                     EAFAIIKAKSRDNARTPMQWDASEHAGFTTGKPWLEVGKSYRDINVETEKEGRIFPFY
                     QRLIALRKELPIIAEGDYRAAFKDSQAVYAFERHLGDQCLLVLNHFYADEVELELPPR
                     YQHGQVLISNYEKVSICEKVILKPYQTLAILADN"
BASE COUNT         3540 a         2051 c         2773 g         3720 t
ORIGIN      
        1 gtagataaac agataacgac aaaaagtttt attgtttatc agaaaaagta atttaatatc
       61 aatatattgt gttcgagcaa aaaacttgac acaatatatt gattttttta gttgtcgtga
      121 tataatagtt ccgtaaggag atttgatatg gtaagtttag aagaagacaa ggtgactgtt
      181 caacctgata ttaaagtgat taaacgagat ggtcgccttg ttaattttga tagtacaaaa
      241 atctatagtg ctttattaaa agcaagcatg aaagtaactc ggatgtcgcc acttgttgag
      301 gctaaattag aggctatttc tgatcgcatt atagcagaaa ttattgagcg ttttccaact
      361 aatatcaaaa tttatgaaat ccaaaatatt gtagagcata agcttcttgc agctaatgaa
      421 tatgctattg caaaagaata cattaattat cgtactcagc gtgactttgc acgttcacaa
      481 gcaacagata tcaatttttc tattgataaa ttaattaata aagatcaaac agttgttaat
      541 gaaaatgcta acaaagatag cgatgttttt aatactcaac gagatttaac tgctggaatc
      601 gtagggaaat cgattggttt aaaaatgtta ccttcgcatg ttgctaatgc tcatcaaaaa
      661 ggagatatcc attaccatga tttggattac agtccttata caccgatgac gaactgctgt
      721 ttaattgact ttaagggcat gttagccaat ggctttaaaa ttggtaatgc tgaagtggaa
      781 agtcccaagt ctattcaaac tgcaacagct cagatctcac agattattgc gaatgtagca
      841 tcaagtcagt acggcggatg cacagctgat cgcattgacg agtttttagc cccatatgcg
      901 gagcttaact ttaaaaaaca tatggctgat gctaagaaat ggatcgttga gactaagaga
      961 gaaagctatg cttttgaaaa gactcaaaaa gatatttatg atgcgatgca gtctttggag
     1021 tatgaaatta atacgctctt tacgtctaat ggtcaaacac catttacttc tttaggattt
     1081 ggtttgggga cgtcttggtt tgaacatgag attcaaaaag ctattttgac cattcggatt
     1141 aatggtcttg gtagtgaaca tcgcacggct attttcccta aattaatttt cacggttaaa
     1201 cgtggcttga atttagaacc agattcacca aactatgata ttaagacttt ggctttagaa
     1261 tgtgcgacta agcggatgta cccggatatg ttatcttatg ataaaattat tgatttgaca
     1321 ggatctttca aatctccaat gggatgccgc tctttccttc aaggctggaa agatgaaaat
     1381 gggcaagatg tgacctcagg ccgtatgaat cttggggttg tcaccctcaa tttacctcgc
     1441 attgccatgg aatcaaatgg cgatatggat aagttttggg agctgtttaa tgagaggatg
     1501 ctaattagta aggatgcttt aatttatcgt gtcgaacgtg tcacagaagc aaaaccagca
     1561 aatgctccta ttctttatca atatggtgct tttggaaagc gtttggagaa gacagggaat
     1621 gtaaatgatc tctttaagaa tcgtcgtgca acagtctctc ttggctatat tggtctttat
     1681 gaagtggcgt ctgtttttta tggtggtcaa tgggaaggta atccagatgc taaagctttt
     1741 accttgtcaa ttgtcaaggc aatgaaacag gcctgtgagg attggtcaga tgaatatggt
     1801 tatcatttct ctgtttattc gactccatca gaaagtttga cagatcgctt ttgtcgttta
     1861 gatacggaaa aatttggcat tgtgacagat attacggata aagaatacta tacaaattct
     1921 tttcactatg atgtgcgtaa gagtccgaca ccttttgaaa aattagattt tgaaaaagat
     1981 tatccagaag caggtgcttc aggtggtttt atccactact gtgagtatcc tgttttgcaa
     2041 caaaatccaa aggccttgga agcggtttgg gactatgctt atgatcgtgt ggggtatttg
     2101 ggaaccaata cgcctattga taaatgctat aattgccaat ttgaaggcga ttttacccca
     2161 acagaacgtg gttttacttg cccaaactgt ggcaataatg accctaaaac agttgatgtg
     2221 gtcaaacgta catgtggtta cttggggaat cctcaggccc gcccaatggt taacggtcgc
     2281 cataaggaaa tctctgcgcg tgtaaaacat atgaatggtt ctactataaa atacccaggc
     2341 ctgtaaactc aagctggggg agagcccttt tgatggaaat atgatatgct taaaaggttg
     2401 cttcatgcaa gcaatctttt attgtttaat ggtttcttag caattgtgag ataagaataa
     2461 ctaatccaaa atgactaatt agctgttagc aatcggacta taagaagaaa gagataagga
     2521 agaaataatg ggaaaatatc aattagacta taaaggaatg cagcaggtag aacgctttca
     2581 tgagaagcat tctaaaaaaa agacggataa aaaatcccgc gttcaagaac ttaaggctcg
     2641 gtttttagag aagtcaaaaa aacaggataa ctaaagtatc cagtaaagaa agaaggttga
     2701 gtatgcttaa tattgggatt gtgggactag gtgctatttc acaaaaagcc tatttaccat
     2761 atatgagaca gctgagtgac attacttggc atctatcaac gcggaatgca gcagtacgtc
     2821 agcaggtcgg tcagttattt ggtcacgcta ttctttacag tgatgtcaag gagttgtcaa
     2881 aaacaaactt agatggggtc tttattcatg cggccacatc ggcccacgca gagttggcta
     2941 gtttattttt aaatcagggg atcccagtct ttatggataa acctatagct gacaattacc
     3001 tgatgacaaa gaacctctat gacttagcca aagaaaatca gacttttctg atggcaggat
     3061 ttaacaggcg ttttacacct cgtgtcaaga agttgtcaag tttgtcaact aagcgtaaag
     3121 tggctgttga aaaaaatgac ttgaaccggc caggagatat gacttttaag ctctttgatt
     3181 tctttatcca tcctttagat acagccttat tcttgacaga agggacctta ctaaaagggc
     3241 attttcaata tcatctggaa gcaggcttac tgagtcaagt tatggtaacc ttgatgaccg
     3301 aaagcatgac gactacagct tctatgaatt tacaatcagg gagtcgccgt gaggtaatgg
     3361 aagttcaacg ggctgaagaa acttatcatt tagagaactt agacgagtta tctatttata
     3421 aaggtactga aaaaagggtg cttggctttg cttcttggga cactaccttg cacaaaagag
     3481 gttttgaaac aatgatcgat gcctttttag aagctattag cacaggcgtc aatcctgtca
     3541 gccctgagtc tagtctttta agccactgga tttgtcaaca gattgctgac tctcagcttt
     3601 cttatggaga gttaacggta gagttgccta aggattagga attgatatgg aaataagacg
     3661 accgacttta aaagataaag acgctgtatt gtcaatgatt aacgaatttt tagagcagaa
     3721 aagtgctaca gatgggttat ggcattttaa cgtcaatgac tttaactatg aaacatggtt
     3781 ggaagattcc ttgcgacagg aaatgggttt gtccagtcaa ggagtgcctg ctattcaata
     3841 cgtcgcattt gatgagagaa gtcaggctat cgggttctta aatcttcgtt tacggttaaa
     3901 tgagagatta cttgaaaaag gtgggcatat tggttactct gtccgtccca gccagcgtgg
     3961 gaaaggttat gccaaggaga tgcttaaaca agctgtcagc tatgcgattt ccaagaatat
     4021 cacgacaatt ttggtaactt gtgatgagac taatattgca agccgagcag ttattgtggc
     4081 taacggcggt atcttggaag atagtcgagg tggaacggaa cgttattgga ttgaagaaca
     4141 aagaggcgat aagtgatggc agaaaagtgt tggaataatc ctaagccaaa ggaatggcag
     4201 gcagaggagt taagtcaagg ccggattatt gactataagg cttttaactt tgttgatggt
     4261 gaaggggttc gtaattccct ttatgtgtct ggttgtttat tccattgcaa ggggtgttat
     4321 aatgctgcga cttggtcttt taaggcggga atgccttata cgcaagaact tgaagagcag
     4381 ataatgacgg atttggcaca gccttatgtt cagggactga cacttttagg tggagaaccc
     4441 tttttgaata cgggtatctt aattcctctg attaagcgta ttcggcgtga attgccagaa
     4501 aaagatattt ggtcttggac gggttatact tgggaagaaa tgatgcttga aacaccagac
     4561 aaactggaaa tgctgtcctt aattgatatt ctggtagatg gtcgttttga tatcacaaaa
     4621 aagaacctca tgttgcaatt tagaggctct tctaaccagc gaattatcga tgttcaaaag
     4681 tctttggctg ctaaagaagt gattatctgg gataagttaa acgatgggga ccaaaccttt
     4741 gaacaaatta gtcgggagga tttgctttaa gaagctgact tatccacatg atttttttga
     4801 aaaggtcatg tgaaaacagg tcctttaagg gtgaattggg gagaaagtaa ttgttattcc
     4861 ctggttttcc ccacattatc ctcatagtta tccacaggtt gtgtataagt tgtagaattg
     4921 tgaataaaga ctaaaaaaga ggcctaggcc tcttttttga tatagacaaa ttcatgatct
     4981 gttgataggt ctggatggta gtaaaaacca gcaaagacaa ggcttttaag gctgtcaaca
     5041 gtttggcaat tattttccat acaggcagtg agaaatgcac ctcgtgcctt ttttgaaatg
     5101 gtggaatggg ttttgaattg gccctctttt tctgccataa acttgggact tatccacaat
     5161 tgtttacagt ctttggaaaa gacatcgtca aattcactgg ataaaaggga aatcacttgg
     5221 ggatgctctt tggcaaactg attataacag ggccgccagt aagacttgag gctttgtcct
     5281 tcaattttta ttctggtgtg aaaatcatgg cggtgctctg cgataggatg attagcggga
     5341 atgataccgt aaaaggatga ggtgatgtaa acctgctggg tgaggtaagc ctgttcttgg
     5401 gtagtgagct tatcgcgctt gatatggcgg tacataaggc cattgaacag ttggtaggca
     5461 gggtaagcca agctttgttg agaggccatg tcttgccagc gttgctgttc ttttttagct
     5521 gactcttctt tgatacgata tgatttggcc aagtcctccg tggtcatagc tgccatgatt
     5581 tttaaaatgg cttggctgtc ttgtggaagg aggtgtgggt gagattcctt agggatcgtc
     5641 atttcttttg cggttggaat taaaaaggtt agcatagtgt tattgtagta aagttacttg
     5701 ctttctgtca agaaaactag tcttgtccaa ggatgacgtt cacacggata ccttcctcgt
     5761 cttctgtcgt gaaagaagaa gaatcttctt gtaaaatagc taggccgtgt aatcttgctt
     5821 ttttgagagt ggctgaaaaa agcaggggcg tctcaatgtg aatggtcaag aaggctaagc
     5881 ctggagcacc ttcttggtgt tttttgaggt aaggggctga ccagtgattg aatgcaaggt
     5941 gatgatagta gttccctgaa gcaatccagc tagcacttgg aatggtcatt ttatcgccta
     6001 aatcaaaaac cttttgatag agaagtgaag aagccagagc atttttgaca ctcagatgga
     6061 catggcgaat gcgtgtgtct tgagctagta aaaagtgctt ggggatatct gtcaactgct
     6121 ccaatatgct tttggtatcc gtgggctcag tcacaccaat aatttgacca ttgtcgcgga
     6181 tatcccaatg ctcaacagct ttgtcatgat aaatttcaat cccattaccc tcaggatcgc
     6241 tgaggtagat agcttcgcta tggccgtgat cagctgctcc ttctaaagag atacttctgg
     6301 ttaagaaatg attgagaaca agtcccaagc tatgacgatc aggaactaaa aaggctgtgt
     6361 gataaagacc ataagcttta tcgcctggta aaggtgtttg tcgtaactct aagataacgg
     6421 ttttaccgtc tgtagtcaat tgacgagatg tcgtgtcttg tgagagaacc tgtaaaccga
     6481 taatacttgt gtaaaaggtt gtcatctttg ctagatcggt aacatttaga gaaaccgttc
     6541 ctaaagagat ggttgagttg taagggtata tcatcctatt acctccaaaa actaattaaa
     6601 tctatcataa tcaatcagtt tcaaaaagaa aagtaggcac aaataagtgg tataggcact
     6661 aaaaagtaac tcatttgtca gccctatctt gtttctgttc tctctaatag tctataatgg
     6721 gttataagtt acaaaaagat actaagagga gctgctgcct atgctgaaaa aaagaccggc
     6781 ttgtcccgta gaaacaacct tatcggtaat cgggaataaa tagaaattat taatattaag
     6841 agacttgcta aaggggacct tgcgttttgg gcagttgaag tcctcaatag gttcggtcag
     6901 tcaaaaggta cttactgccc aattaagggc tatggaagca gacggattgg ttcatcggga
     6961 agtgtatgca gaagtgccac ctcgggtgga gtattcctta acagaaactg ggttgagctt
     7021 agctcctgtt attgaagcga tgtcggattg gggccaaact taccaagaaa aacatgccga
     7081 tttatttgaa gaaggctaga ggaaagagtt gtccgagcag actgagactt agagaagctg
     7141 agagttttta aaaaatgata ttatcacatt ctttacagtt tgataaaatt actttttttc
     7201 tacaaaaaga cttgcgctca tctcacgaat atgataatat aatatcaagt ctgtttgaga
     7261 aaacagaagc tcaggtcaag taagtgcaca cttacaataa cacagttgat aatggggaaa
     7321 ctcagagtca agaaaaagaa aagcaagcct tgcattttgc aaggcttttt tctgtcttta
     7381 ttttggtctg cgccgagcaa aatctaaaaa tttaaatttt tccagtttat ggcgactctc
     7441 ggtgaattgg aattggttat tattggaaag gtagactttg gatttgacgg aaacaacatg
     7501 ttggtctgat ccaaggtcaa gtaaaatttt atcacgatcg gttatttggt caatggtaac
     7561 ctctttgagg gcaaagtcaa tggcgagatg gagttccttt tccaaatatt gataaataga
     7621 atgctctgcg atttcccttg tcattattgg caccaaagtt ttactaaggt aatcaatgtc
     7681 taagacggaa gccacaccct caacaacgcg ttggcgtgta acacgccaga ccaagctgtt
     7741 tttgctaaaa ccggtcaatt ttgaaagtgt ctcatccaca atcagtttat caatggcaat
     7801 gacgttggtc ttagaatcta gatttgagta agagacgagt tcctgataac tagttagttc
     7861 tgagataggg aacatgatct gatgatgtct aatcacttgg gtgccacgtc cttgtttttt
     7921 gaggataaga cctgcctttg tgagaagtga gagagccttg cggacggtat ctcggctagc
     7981 ttggtattgt tgactcaatt ctatttcagt aggtaaaaaa tccccttctt tgtaaaagtc
     8041 tttgtttatt tttgtttcga ggtctttata aatacgttca tactttgtca tagggtaaat
     8101 tacctttcca tttgtctatt tttttacaga caacaccttt tattttacca aaataatggc
     8161 ttttttggca caaacaactt gtagacaaat ttgcaatcga ttacaataaa ggtgtcagtt
     8221 agattctatc taagaatttg aatatttcat tttgacgttg cttgttaaaa gcaactagaa
     8281 caaaggacta ggggatgtca catgggaaaa tttgaacagg atgctaagag tcttctaact
     8341 gctattggtg gtaaagaaaa catcaaggtt gtcacacact gtgcaacgcg tatgcgtttt
     8401 gttttgaatg ataataataa ggcaaatgtc aaagagattg aaaaaatctc tgtagttaaa
     8461 gggacattta ccaatgctgg gcagtttcag gtaatcattg gtaatgatgt tccagttttt
     8521 tataatgact ttacagctgt ttctagtatt gaaggggtgt ctaaagaagc tgccaaatca
     8581 gcagctaaaa gtaatcaaaa tgccttacaa cgggtgatga ccatgttggc tgagattttc
     8641 acacctatta ttccggcgat tattgttggg gggcttattt taggtttccg taatattttg
     8701 gagagtgtgc cttttgaatt tcttgggcag caggtcgaaa aagggaaatt agtttttgat
     8761 gcagctgggg atcctgtttg gaatacgatt gtgagggtat ctcctttctg gtcaggggtt
     8821 aaccatttct tgtggttacc aggggaagct attttccact tcttaccagt tgggattact
     8881 tggtctgtga cgcgtaagat gggaaccact caaattttag ggattgtcct tggtatctgt
     8941 ttggtgtcac cacaattatt gaatgcctat gcggtggcag gaacgcctgc tgctgagatt
     9001 gccaaaaact gggtttggga ttttggtttc tttaccatta atcgtattgg gtatcaggca
     9061 caggttattc cagccctttt agctggtctg tcccttgctt atcttgaaat tttctggcgt
     9121 aaacggattc cagaagtggt ttcaatgatt tttgtgccat tcctttcttt gattccagct
     9181 ttgattttag cgcatacggt attggggcca atcggttgga ctattggtaa agggatttcc
     9241 tttgttgtgt tagctggatt gactggtcct gttaaatggc tattcggtgc tatctttggt
     9301 gccttgtatg ctccgctagt tattactggt ttacatcaca tgacaaatgc cattgatacc
     9361 caattaattg ctgatactgc aactcgtaca actggtttgt ggccaatgat tgctctttca
     9421 aatatcgctc aagggtcagc cgtttttgct tactatttaa tgaatcgtca tgaagaacgt
     9481 gaggctgaaa tatcgcttcc tgcagcaatt tctgcttacc ttggggtaac tgagcctgcc
     9541 ttatttgggg ttaatgttaa atacgtttat ccctttgtag ccggaatgat tggctcaggt
     9601 attgcgggtc tcttatcaac aacctttaat gttcaggcaa attctattgg tgttggtggt
     9661 ttaccaggtt tcatggctat caatgtgaag tacatgattc cattcttcat ctgtatggca
     9721 gtagccattg tggtgccgat gtttttaacc ttctttttcc gtaaatcaca tatcatgact
     9781 aagacagaag atgaagctaa actacctgag acacccgttt cggatgctcc tgtagcaact
     9841 gctccacata agactatgca aggaacagtt atcactttaa caagcccttt aacgggtgaa
     9901 gttaaagcgt tgtctgaagc tgttgatcct gtctttgcac agggagttat gggccaaggt
     9961 gctcttcttc aaccgacaga aggggtgtta gtagcgcctt gtgatgctga agtatcggtc
    10021 ttgttcccaa ctaaacacgc tatttgtttg gtgacgactg aaggtttgga attattgatg
    10081 catattggca tggatacggt taacttagat ggtcaaggat ttgaagcttt ggtgaagcaa
    10141 ggtgatcagg ttaaggctgg acaaacattg attcaatttg atatagcagc aatttctgaa
    10201 gctggatacg ccactgaaac gcctcttgtg gtgactaatc aagatgtttt tacggtaact
    10261 gttgaaggta gtttaccgcg tcagattaag gttaatgata agttagcagt agcggtgaaa
    10321 aagtaggttt actaagagaa aacgttcagg aactgagatc agttcctgaa actattttag
    10381 ggaggaaaac tcatgacaat tgataaaaag aaagtcgtct atcaaattta cccaaaatcc
    10441 tataaggaca cgactggaaa tggtgtggga gacttgctag ggatcattga taaattgcct
    10501 tacttacaag aactaggaat agatatgatt tggttgaacc ctttctaccc tagtccacaa
    10561 cgagacaatg gctatgatgt ttcagattat acggcggtca accctgattt tggaacaatg
    10621 gctgattttg aaaatttggt aaaagctgct aaggagcatc agattgagtt aatgttggac
    10681 atggttttga atcactgttc cacagaccac gagtggttcc aaaaagcttt agcaggagac
    10741 ccttattatc aggatttctt tatcttgaga gatcagccga ctgattgggt ttccaaattt
    10801 ggtgggaatg cttgggcgcc ttttggagat acaggcaaat actacttaca cttgtttgat
    10861 gtgacacagg ctgacttgaa ttggcggaac ccacatgttc gtgaggaatt ggctaaagtg
    10921 gttaattttt ggcgagataa aggagtgaag gggttccgtt ttgatgtgat taacctgatt
    10981 gggaaagatg aagagctggt ggattgtccg gtcaatgatg gtaagccagc ttatacggat
    11041 cgtcctatta ctcacactta tcttcatgat ctcaatcaag ccagttttgg tcaggatgat
    11101 tcgtttatga cagtagggga aatgtctgca acgactattg acaactgtct tttatacacg
    11161 gctcccgaac gtgaggagct atccatggct tttaatttcc accatctaaa agttgattat
    11221 gagaacggtc agaaatggac tattatggct tttgattttg cagcgctgcg agacttattc
    11281 catgcttggg gtgaaggcat gagtcagggc aatgggtgga atgccttgtt ctacaataac
    11341 catgatcaac cacgtgccct gaatcgtttt gttgatgtaa cacatttccg aaacgaaggt
    11401 gcgacgatgt tagcagcttc catccatttg tcacgaggaa cgccttacat ttatatgggt
    11461 gaggagattg gcatgcttga tccagacttt gatagtatgg atgattatgt ggatgtggaa
    11521 agtctcaatg cttactcaag cttattagtc tcaggtaaaa gtgcagaaga agcctttgcc
    11581 attatcaagg ccaagtcaag ggacaatgcc agaacaccaa tgcaatggga tgctagtgaa
    11641 catgctggct ttacgactgg taagccttgg ttagaggttg gcaaatctta tcgagacatc
    11701 aatgtcgaaa cagaaaaaga gggacgtatt tttcctttct accaacgctt gattgctttg
    11761 cggaaggaac tccctattat tgctgaaggg gactatcggg ctgcttttaa agatagtcag
    11821 gctgtctatg cctttgaacg ccatttaggt gaccagtgtt tgcttgttct caatcatttc
    11881 tatgctgatg aggtcgaact ggaattacct ccacgttatc aacatggaca ggtcttaatc
    11941 agcaactatg agaaagtttc tatttgtgaa aaagtgatac tgaaacctta tcagacactt
    12001 gctatcttag ctgataacta actaaaagcc tgaggttagg cctcaggctt ttgctgattg
    12061 tcacgaaaaa agaagttttc tcat
//
LOCUS       DAXWIQ010000047        11669 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000047 DAXWIQ010000000
VERSION     DAXWIQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.047"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            5..78
                     /locus_tag="ACGEUI_001449"
     tRNA            5..78
                     /locus_tag="ACGEUI_001449"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Asn,seq:gtt)
     gene            158..231
                     /locus_tag="ACGEUI_001450"
     tRNA            158..231
                     /locus_tag="ACGEUI_001450"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:192..194,aa:Arg,seq:acg)
     gene            257..808
                     /locus_tag="ACGEUI_001451"
     CDS             257..808
                     /locus_tag="ACGEUI_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFF8166276.1"
                     /translation="MSCCFFVVLFILGIVKKKRRLKMSIETRAAFEKVKPIILKLKRH
                     YYIQLWDRDDWLQEGHIILLQLLERYPELIEEEERLYRYFKTKFSSYLKDLLRRQESQ
                     KRQFHKLAYEEIGEVAHAIPSRGLWLDDYVAYQEVIASLENQLNSQERMQFQALIRGE
                     RFKGRRALLRKISPYFKEFAQQL"
     gene            993..1742
                     /gene="truA"
                     /locus_tag="ACGEUI_001452"
     CDS             993..1742
                     /gene="truA"
                     /locus_tag="ACGEUI_001452"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFF8166277.1"
                     /translation="MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKI
                     IIHGAGRTDAGVHAYGQVIHFDLPQEQEVEKLRFALDTQTPEDIDVVNIEKVADDFHC
                     RYQKHLKTYEFLVDNGRPKNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAA
                     GTSVQNKVRTITKATVSRDEKTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQ
                     VKVILSSKNRQLAGPTISGNGLYLKEICYEN"
     gene            1732..2496
                     /locus_tag="ACGEUI_001453"
     CDS             1732..2496
                     /locus_tag="ACGEUI_001453"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFF8166278.1"
                     /translation="MKTDYIVTISGNDILSGGGLYADLATYIRYDLQAFVAVTCLTTR
                     SEEGFSLFPVAKEIFRDQLNSFTNAPISAIKIGLLPNAEMCEIVLDFIKGHLGIPVVL
                     DPVLACKEIDDVKIVPLRQEILQLLPYVTVVTPNLVEAQLLSQKEIVSLKDMQEAAKY
                     FYQLGAKQVVIKGGNRFSQKKAIDLFYDGKEIVTLECPVLEKNNIGAGCTFASSIASQ
                     LVKKKTPLEAVKNSKELVYQAILQSDRYGVKQSYAK"
     gene            2486..2956
                     /locus_tag="ACGEUI_001454"
     CDS             2486..2956
                     /locus_tag="ACGEUI_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8166279.1"
                     /translation="MQNSKIRQMSLTGILTALVVVLGRFVMLPTPTGFLTLLDAGIYA
                     VSFSFGSAQGAIVGGLSGFLIDLVAGYPQWMFHSLIAHSVQGYFAGWRGRKRWLGVVI
                     GSFIMIFWYFLGSLMLGYGLSGSLAGIWGNVMQNTLGLFVGFIIFKAILRQKKR"
     gene            2970..3530
                     /locus_tag="ACGEUI_001455"
     CDS             2970..3530
                     /locus_tag="ACGEUI_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFF8166280.1"
                     /translation="MLNNLEKQTREIVIDVVERSAIQPGNLFVLGLSSSEILGSRIGK
                     QSSLEVGQIVVEVVLDELNKRGVHLAVQGCEHVNRALVVERHVAESKQLEIVNVVPNL
                     HAGGSAQMAAFQLMSDPVEVEEVIAHAGLDIGDTAIGMHIKRVQIPLIPCQRELGGAH
                     VTALASRPKLIGGARADYNMDIIRKS"
     gene            complement(3595..4440)
                     /locus_tag="ACGEUI_001456"
     CDS             complement(3595..4440)
                     /locus_tag="ACGEUI_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFF8166281.1"
                     /translation="MTFILHYLEQSHIENIGLTIFKKLVSLIILLLFFAILKRVTNYL
                     FEKTINKSFAYSRQSEARKKTLSKLTHNILNYLLYFLLIYWILSLFGIPVSSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILFENQFEVGDNVTISDIEGSVFGVGIRTTQIRGFD
                     GTLHFIPNRSITVVSNKSRGNMRALIEIPLYSTVNLSQVTRIIDEVNQKELPNHPQIV
                     GKPNILGPQNNSNGQFTFRIAIFTENGEQFKIYHTFYRLYQEALLKEGIQLPTAISYS
                     ITSKS"
     gene            4761..6044
                     /gene="tig"
                     /locus_tag="ACGEUI_001457"
     CDS             4761..6044
                     /gene="tig"
                     /locus_tag="ACGEUI_001457"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFF8166282.1"
                     /translation="MSTSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGF
                     RKGHMPRPVFNQKFGEEVLYEDALNIVLPEAYEAAVTELGLDVVAQPKIDVVSMEKGK
                     EWTLSAEVVTKPEVKLGDYKNLVVEVDASKEVSDEDVDAKIERERQNLAELIIKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEDQLVGAKAGDEVEVNVT
                     FPESYQAEDLAGKAAKFMTTIHEVKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKEL
                     EAAQETAYDDAVEGAAIELAVANAEIVDLPEEMIHEEVNRSVNEFMGNMQRQGISPEM
                     YFQLTGTTQEDLHNQYSAEADKRVKTNLVIEAIAKAEGFEATDSEIEQEINDLATEYN
                     MPADQVRSLLSADMLKHDIAMKKAVEVITSTASVK"
     gene            6261..6836
                     /gene="rpoE"
                     /locus_tag="ACGEUI_001458"
     CDS             6261..6836
                     /gene="rpoE"
                     /locus_tag="ACGEUI_001458"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFF8166283.1"
                     /translation="MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQN
                     YLGKSDAGIRHALPFFYTDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     AQKRKKKRVNAFMDGDEDAIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDS
                     ELNEIIPEDDFEEVDINEEDEEDEEDEEPVL"
     gene            7093..8697
                     /locus_tag="ACGEUI_001459"
     CDS             7093..8697
                     /locus_tag="ACGEUI_001459"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFF8166284.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKEKIKRAASTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSENVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPVE
                     QGIKNKLAQFCDVNSEAVIESRDVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTE
                     WSAMVDKVMNLRKTTKIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDV
                     TVDNAADLLGDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGICLGMQLTCVEFA
                     RHVLNMEGANSFELEPSTKYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAY
                     NNQEVVQRRHRHRYEFNNKFRPEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYH
                     PELQSRPNRPEELYTAFVTAAIKNSN"
     gene            8865..9791
                     /locus_tag="ACGEUI_001460"
     CDS             8865..9791
                     /locus_tag="ACGEUI_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8166285.1"
                     /translation="MKTIRIAKYLGILFLLITLISVGASFYFFHVAQIREEKSFINNK
                     KRSTNNPLYPAEQSFDALPYEKRQLTNRGLKQVGWYLPAAQKTKKTAIVVHGFTNDKE
                     DMKPYAMLFHDLGYNVLMPDNEAHGESEGNLIGYGWNDRLNVMAWTDQLIKENPESQI
                     TLFGLSMGAATVMMASGERLPAQVTSLIEDCGYASVWDELKFQAKAMYNLPAFPLLYE
                     VSALSKIRAGFSYGEASSVKQLAKNKRPTLFIHGDKDDFVPTKMVYDNYKATKGPKEI
                     LIVKGAKHAKSFETNPEQYQKKIAAFLKKVEK"
     gene            9845..9930
                     /locus_tag="ACGEUI_001461"
     tRNA            9845..9930
                     /locus_tag="ACGEUI_001461"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9879..9881,aa:Leu,seq:aag)
     gene            10137..11018
                     /locus_tag="ACGEUI_001462"
     CDS             10137..11018
                     /locus_tag="ACGEUI_001462"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFF8166286.1"
                     /translation="MAIVSAEKFVQAARENGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVCQSLITNLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKTAEVVKIAHAKGVSVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPENWEGLALDHLEKLTAAVPGFPIVLHGGSGIPDDQI
                     KEAIRLGVAKVNVNTESQIAFSNATREFARNYEANEAEYDGKKLFDPRKFLAPGMKAV
                     QGAVEERIDVFGSANKA"
     gene            11400..11588
                     /gene="rpmB"
                     /locus_tag="ACGEUI_001463"
     CDS             11400..11588
                     /gene="rpmB"
                     /locus_tag="ACGEUI_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFF8166287.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTILVDGKP
                     KKVWASARALKSGKVERI"
BASE COUNT         3812 a         1816 c         2530 g         3511 t
ORIGIN      
        1 tttatccgcc atagctcagt tggtagtagc gcatgactgt taatcatgat gtcgtaggtt
       61 cgagtcctac tggcggagta gagagaggta ggaagcgtcc tatctttttt tgcattttag
      121 agaagagtgt ggtaagatag aggcaagata tgcgttggca cccttagctc aactggatag
      181 agtacctgac tacgaatcag gcggttagag gttcgactcc tctagggtgc ataatgaaaa
      241 aaacaacagg acattaatgt cctgttgttt ttttgtggtt ctctttatac tagggatagt
      301 taaaaagaaa aggagactca aaatgtcgat agagacaaga gcagcgtttg aaaaagttaa
      361 gcccattatt ttgaaattaa agcgacacta ttatattcaa ttgtgggata gagatgactg
      421 gttacaagaa ggacatatta tcttattaca gttactagaa aggtacccag agttaattga
      481 ggaagaagag cgtttatatc gctattttaa aacaaaattt tcatcttatt tgaaagattt
      541 attacgccgt caagaaagtc aaaagcgtca gttccataag ttagcatatg aagagatagg
      601 ggaggttgca catgccattc catcgagagg gttatggcta gacgactatg tggcttatca
      661 agaggtaata gctagcttag agaaccaatt aaattcgcaa gagcgtatgc agtttcaagc
      721 acttatcagg ggcgaacgtt tcaagggaag acgtgctcta cttaggaaga tcagtcccta
      781 ttttaaggaa tttgcacagc agttgtaagc aagacctata aggaattggg ggtaaatcta
      841 tgaaaaaagg agagagtttg gaggaaatag ctttcttata gcttttaaat cgctagtagc
      901 ttagagtaag cttcaatacg tctttcattt gtttgggtac agtttgatta aaaatagttt
      961 ctcttacgtt taagaaatgt taaaatagta acatggtaag atacaaagca acgatttcct
     1021 atgatgggac tcttttttcg ggttttcaaa gacaaagaca tttacgtacg gttcaagaag
     1081 agattgaaaa aacactctat aaattaaata acggtacaaa aattataatt catggtgcag
     1141 gacgtacgga tgcaggagta catgcttatg gacaggtgat ccattttgat ttgcctcaag
     1201 aacaagaagt tgaaaaattg cgatttgctc ttgatacaca gactcctgaa gatattgatg
     1261 ttgttaatat agaaaaagtg gctgatgatt ttcattgtcg ttatcagaag catttaaaaa
     1321 cttatgaatt tttagttgat aatggtcgac ctaaaaatcc gatgatgcga cactatacca
     1381 ctcattaccc ttatactctt aatattaaac ttatgcaaga agctattaac ggtctggtag
     1441 gtacacacga ttttactggt tttacagcag caggaacttc ggtacaaaat aaggtgcgaa
     1501 cgattaccaa agcaacggta tctcgggatg aaaaaactga ttttttggtg tttacttttt
     1561 caggaaatgg ttttctctat aagcaggtga ggaatatggt gggaactttg ctgaaaattg
     1621 gtaatggtca aatgccagtt gaacaagtta aagtgatttt gagctccaaa aatcgtcagc
     1681 tagctggacc aacgatatct ggaaatgggc tttacttgaa ggagatttgt tatgaaaact
     1741 gattatatcg tgactatatc tgggaacgat attttaagtg gaggtggtct ctatgctgat
     1801 ttagcgactt acataaggta tgacctacag gcctttgtag cggtaacttg tttaactact
     1861 agatctgaag agggctttag tctatttccg gtagctaaag aaattttccg tgatcagttg
     1921 aatagcttta cgaacgctcc aatttctgct attaaaattg gtttgttacc taatgcggaa
     1981 atgtgtgaaa tagttttgga ttttataaag gggcatctgg gaatcccagt ggttttagat
     2041 cctgttctag catgtaaaga aatagatgat gttaaaatag ttcctctaag acaggagatt
     2101 ttacaattat tgccttatgt gactgttgtt actccaaatt tggtagaggc tcagctatta
     2161 tctcaaaaag agattgtttc tttaaaagat atgcaagaag ctgctaaata tttttatcaa
     2221 cttggggcaa aacaggtggt tatcaagggt ggtaatcgat ttagtcagaa aaaagctatt
     2281 gatctttttt atgatggtaa agagattgtt accttggaat gtcctgtact tgagaaaaat
     2341 aatattggcg caggctgtac ttttgcatct agtatcgcta gtcagttagt aaagaaaaaa
     2401 acgccactag aagctgtgaa aaactcaaaa gagttagtct accaagctat tttacaatct
     2461 gatagatacg gagtaaaaca aagttatgca aaatagtaag atacgacaaa tgagtttaac
     2521 agggatattg acagcattgg ttgttgtttt aggaagattt gtcatgctac ctactccaac
     2581 aggtttttta acgctgttgg atgcaggaat ttatgctgtc agcttttcct ttggatcagc
     2641 tcaaggagct attgtgggag ggttatcagg ttttttaatc gatttagtgg caggataccc
     2701 tcagtggatg tttcatagtt tgattgctca cagtgtccaa gggtattttg cgggatggag
     2761 agggcgcaag cgttggcttg gtgttgtgat aggttctttt atcatgattt tttggtactt
     2821 tttaggctct cttatgcttg gttatggcct ttcgggatct ttagccggta tttggggaaa
     2881 cgtcatgcaa aatacattag ggctttttgt tggttttatt atctttaagg ccatattaag
     2941 gcaaaaaaag agataaggag agtgagaagt tgttgaataa tttagagaaa caaacaaggg
     3001 aaatcgtcat tgatgttgta gaacgctctg ctattcaacc aggtaatctt tttgttctag
     3061 gtctttcatc tagcgaaatt ttgggtagta gaattggaaa acaatctagt ctagaagtag
     3121 gtcaaatagt tgttgaagtt gtgttagatg aattaaataa gcgtggtgtt catttggctg
     3181 tacaaggttg tgaacacgtc aatcgtgcct tggtagtaga gaggcatgtg gcagaatcta
     3241 agcagttaga gattgtcaat gtggttccga atcttcatgc tggtggcagt gctcagatgg
     3301 ctgcttttca acttatgtca gaccctgtcg aagtagaaga agttattgca cacgctggac
     3361 ttgatatagg tgatactgct ataggaatgc atattaagcg tgttcagatc cctctgattc
     3421 cttgtcaacg agagctagga ggtgctcatg tgacagcttt ggctagtcga ccaaaattaa
     3481 ttggtggagc tagggctgat tacaatatgg atattatccg taaatcctaa accctttttt
     3541 ctggaataaa acaattggtg ttttttctag aaaaaaggat tttttaagat tatattaaga
     3601 ttttgaggtg atagaatagc tgattgcagt cggtaattga atgccttctt tgagtaaagc
     3661 ttcttgataa agtctataaa aagtatggta aatcttaaat tgttctccat tttctgtgaa
     3721 aatagcaatt ctaaaagtga actgtccatt agagttattt tgaggcccta aaatatttgg
     3781 tttgccaacg atttgaggat ggtttggcaa ttccttttga ttcacttcat caatgattcg
     3841 agtgacttgg ctcaaattaa cagtggaata aagaggaatt tcaattaagg cacgcatatt
     3901 accacgagat ttgttgctga caacggtgat gctacggttt gggatgaaat gtagtgttcc
     3961 atcgaatcca cgaatttgag tagttctaat tccaacacca aagacactgc cttctatgtc
     4021 agagatagta acgttatctc ctacttcaaa ttgattctca aataagataa aaaagccatt
     4081 gacaacgtca gataaaaatc cttgtgcacc taacccgatt gctactccag caattccagc
     4141 tcctgctaga agactagaga caggtatgcc aaataagcta agtatccaat aaatgagaag
     4201 aaaatataat aagtaattta aaatgttgtg agtgagctta gagagtgttt ttttgcgagc
     4261 ttcactttgt cttgaataag caaacgattt gttaatggtt ttttcaaaaa gatagttagt
     4321 aacacgtttt aaaatagcaa aaaagagtaa tagaataatg agagacacta gctttttaaa
     4381 aatcgttaag ccaatatttt cgatatgaga ctgctctagg taatgtaata taaatgtcat
     4441 gagaaatcct ttctttgata gtttatcaaa aaaaaaccaa tcttgaaaag agataaatta
     4501 atattttaac caaagttatg ataattgttt tatcataaag aatgccatag tcatccgttg
     4561 tgataatgct tgaaaatagt cagttagtaa gcgaaaaagt ctaacattaa cctttataaa
     4621 ttgaaaataa ctgttagtag actaatgaca ggtagatact cttaatagcg cgtttgattt
     4681 cacgcataaa aagctttaaa aacgctttaa attatgataa aatgaactgt atcgaaatta
     4741 ttttaaggag aaatgactta atgtctacat catttgaaaa caaagctaca aatcgtggcg
     4801 tgattacatt tacaatcagt caagataaaa taaaaccagc tcttgataaa gcttttaata
     4861 aaatcaaaaa agacttgaat gcaccaggtt tccgtaaagg acacatgcct cgtccagtct
     4921 tcaatcaaaa atttggtgaa gaagttcttt atgaagatgc tttgaatatt gtattgccag
     4981 aagcttatga agcagctgtg acggaacttg gtcttgatgt ggttgcacaa ccaaaaatcg
     5041 atgttgtgtc aatggaaaaa gggaaagagt ggacactttc tgctgaagtt gtgacaaaac
     5101 ctgaagtgaa acttggtgat tacaaaaacc tagttgtaga agttgatgct tcaaaagaag
     5161 tttcagatga agacgtggat gctaaaattg agcgcgaacg tcaaaacctt gcggaactca
     5221 ttattaaaga cggtgaagca gctcaaggtg acactgttgt gattgacttt gttggttcag
     5281 ttgatggtgt tgagtttgat ggcggtaaag gagataactt ctctcttgaa cttggttcag
     5341 gacaatttat cccaggtttt gaagatcaac tagttggtgc taaagctggc gatgaagtag
     5401 aagttaatgt cacattccca gaatcttacc aagcagaaga tcttgcaggt aaagccgcta
     5461 aatttatgac aactattcac gaagtcaaaa caaaagaagt accagagctt gatgatgagc
     5521 ttgcaaaaga tattgatgaa gatgttgaca cacttgaaga cttaaaagta aaatatcgta
     5581 aagaacttga agcagctcaa gaaactgctt atgatgatgc tgttgaagga gctgcgattg
     5641 aattagcagt tgcaaatgct gaaattgttg atttacccga agaaatgatt catgaagaag
     5701 tcaaccgttc agtgaatgaa tttatgggca acatgcaacg tcaaggaatc tcacctgaaa
     5761 tgtacttcca attgactggt acaactcaag aagatttaca taaccaatat tcagctgaag
     5821 ctgacaaacg tgttaaaaca aaccttgtta ttgaagcaat tgctaaagca gaaggttttg
     5881 aagcaacaga tagtgaaatt gaacaagaaa ttaatgacct tgcaacagaa tataacatgc
     5941 cagctgacca agttcgttct cttctttcag cagatatgtt gaaacatgat attgcaatga
     6001 aaaaagcagt tgaagtgatt acaagcacag caagcgttaa gtaatcttat taaggaacaa
     6061 aacgtacaag ccggattaga acgtctttct agtctggttt ttctttttct aaagaaataa
     6121 taaggaatta gttaaacgtt attttaatga attagcttta aagatttgtc aagaagaagt
     6181 gatatgagtt gataaaagtt ttgacgaatt acttttttta gcgtaaaatg gtagagaatg
     6241 ataaaataag gagaactgcc ttgaaattag acgtatttgc aggacaagaa aaaagcgagc
     6301 tttccatgat tgaagtggcg cgtgccattt tggaagaacg cggtcgcgac aatgagatgt
     6361 actttagcga tttagtcaat gagattcaaa attacttagg aaaatctgat gcaggtattc
     6421 gtcatgcatt accatttttc tatactgact taaatacgga tggctcattt attcctctag
     6481 gtgaaaacaa atggggactt cgttcgtggt atgctattga tgaaattgat gaagaaatta
     6541 ttactctaga agaagatgaa gatggcgcac aaaaacgtaa gaaaaaacgc gttaatgctt
     6601 tcatggatgg agatgaagac gctattgatt atagagatga tgatccagaa gatgaagatt
     6661 tcacagaaga atcagctgag gttgaatatg atgaagaaga tccagatgat gaaaaatcag
     6721 aagtggagtc atatgattca gaattgaatg aaattatccc tgaagatgat tttgaagaag
     6781 ttgatatcaa tgaagaagac gaagaagatg aagaagatga agagccagtt ctctaatgtc
     6841 tttagttcag aaataaaaat gtaattatca tttgacaaat tgtctattat agtttatatt
     6901 attattcggg cacctcttta tgaggtcagg caaaagctct ctagttaata gggggctatt
     6961 tttgttttct agaataatgc cagtttgcct ctatatttag aaaactaagg tgatacctaa
     7021 ccaatgtgga accaactaat agggaattaa caagaaagca atgcttttat tcagcttata
     7081 taaaggagta ctatgacgaa atacattttt gtaactggtg gtgttgtatc atcaattggc
     7141 aaaggaattg ttgcggcaag tcttggccga ttgttaaaaa atcgtggact taaggtgacg
     7201 attcaaaaat ttgatcctta tattaatatt gatccaggga cgatgagccc ctatcagcat
     7261 ggagaagtat atgtgacaga tgacggtgct gaaaccgact tggatttggg tcactatgaa
     7321 cgttttattg atatcaatct taataaatat tccaatgtta caacaggtaa aatttatagt
     7381 gaagtccttc gtaaagaacg taaaggcgag taccttggag cgactgttca ggtgattcct
     7441 catatcacag atgcccttaa agaaaaaatc aaacgcgcag cttcaacaac ggactctgat
     7501 gtaattatta ctgaggtcgg ggggactgtt ggcgatattg aaagtttacc gttcttagaa
     7561 gctcttcgtc agatgaaagc tgatgttggt tctgaaaatg tgatgtatat tcacaccacc
     7621 ttgttgccat atttaaaggc agcgggtgaa atgaaaacta agccaacgca acattccgtt
     7681 aaagaattgc gtggtttagg gattcaaccg aatatgttgg ttattcgtac ggaagagcca
     7741 gttgagcagg ggattaaaaa taaattggct cagttctgtg atgtgaattc agaagctgtt
     7801 atcgagtcac gtgatgtaga acacttgtat caaattccat tgaacttgca agctcaatca
     7861 atggatcaga ttgtttgtga tcatttgaaa ttgaatgcgc cacaagctga tatgaccgag
     7921 tggtcagcta tggttgacaa ggtgatgaat ttaagaaaaa caacaaaaat tgccttggtt
     7981 ggtaaatatg tggaattgcc agatgcctac ttatctgttg tggaagcctt gaaacattct
     8041 ggttatgcca atgataccgc aattgaccta aaatgggtca atgctaacga tgtgacggtg
     8101 gataatgcag ctgacttgtt gggagatgcg gatgggatta ttgtaccagg tggttttggt
     8161 caacgtggta cagaaggcaa gatccaagcg attcgctatg cccgcgaaaa tgatgtgcca
     8221 atgcttggta tttgtcttgg catgcagttg acttgtgttg aatttgcccg ccatgtcctc
     8281 aatatggaag gtgcaaactc ttttgaatta gagccaagta ctaaataccc catcattgat
     8341 atcatgcgtg atcaaattga tattgaagat atgggaggta cgcttcgtct gggattgtac
     8401 ccatgtaaat tgaaaccagg ttcaaaagca gcaatggctt acaataatca agaggtggtg
     8461 caacgtcgtc atcgtcaccg ttatgagttc aacaacaaat tccgtccaga atttgaagca
     8521 gctggttttg ttttctcagg ggtatcacct gacaaccgct tagttgaaat tgtagaattg
     8581 aaagagaaaa aattctttgt agcagctcaa taccacccag aattgcaaag ccgtccaaat
     8641 cgtcctgagg agctatacac cgcttttgtt acagctgcta ttaaaaatag caattagtgg
     8701 ttttaagggt aagtttgtgg cattctactt ggctggcaga tactgataaa gattactagc
     8761 tattaaaggg atcttttcta taaaaatagc attagaaaaa gaaaaacgtg atatttttag
     8821 gttagcactt tctttttcag tcattttttt gtaaaatact tggtatgaaa actattcgaa
     8881 tagcaaagta tttaggaatt ctattccttt tgataactct tattagcgta ggtgctagtt
     8941 tttatttctt tcatgttgca caaataagag aagagaaatc gtttattaat aataagaaac
     9001 ggagtacaaa taatccatta tacccagctg aacagtcttt tgacgcttta ccttacgaaa
     9061 aacgtcaact aacaaatcgt gggttaaaac aagtggggtg gtacttacca gctgctcaaa
     9121 aaacaaaaaa gacagctatt gttgttcatg gttttacgaa tgacaaagaa gatatgaagc
     9181 catatgccat gctttttcat gatttgggct ataatgtctt aatgccagac aatgaggccc
     9241 atggggaaag tgaagggaac ttgattggtt atggctggaa tgaccgcctt aatgtcatgg
     9301 cttggacaga ccaactgatt aaggaaaacc ctgaaagcca aatcacactc tttggcttat
     9361 ctatgggtgc tgcaacagta atgatggcaa gtggtgagcg attgcctgcg caagtcacct
     9421 ccctcatcga agattgcggt tatgccagtg tttgggacga attgaagttt caggccaagg
     9481 ctatgtacaa cttgcctgcc tttcctttac tctatgaagt ctctgcctta tctaagattc
     9541 gagcaggttt tagttacgga gaagcgagct cagtgaaaca gctggctaaa aataaacgtc
     9601 caactttatt tatccacggt gataaggatg attttgttcc tacaaaaatg gtttatgaca
     9661 attataaggc cacgaaaggt cctaaggaaa tcttgattgt taaaggggca aaacacgcca
     9721 aatcctttga aacaaaccca gaacaatacc agaaaaaaat tgccgctttt ttgaaaaaag
     9781 ttgagaaata agtattgaca gttaaactca cagttgctat aataatttat gttactgatg
     9841 agtagcgggg atggcggaat tggcagacgc gcaggactaa ggatcctgtg accgctttag
     9901 gtcgtgtggg ttcaagtccc actctccgca taatgtaaga ggtgaatcat tgattcgtct
     9961 cttttttttg gttaaatgaa ggataagaag gcatagattt actggagaaa acttttgata
    10021 atctgctttt ggaaaacgct ttaagcttgt gaaaggctag aaatttatgg aaaattgtgc
    10081 tagaatgaac aatgtaaacg gggagttatc ccgaaaaata agaggaggcc ttacaaatgg
    10141 caatcgtttc agcagaaaaa tttgtccaag cagctcgtga aaacggatat gctgttggtg
    10201 gatttaacac aaacaactta gagtggactc aagcaatctt gcgtgcagca gaagctaaac
    10261 aagctccagt tcttatccaa acatcaatgg gtgcagcaaa atacatgggt ggttataaag
    10321 tatgtcaatc tcttattact aaccttgttg agtcaatggg tatcactgtt cctgttgcta
    10381 tccaccttga tcatggtcac tacgaagatg ctcttgagtg tatcgaagta ggttacactt
    10441 caatcatgtt tgacggttca caccttccag ttgaagaaaa ccttgctaaa actgcagaag
    10501 ttgttaaaat tgcacatgct aaaggcgttt cagttgaagc tgaagttggt acaatcggtg
    10561 gtgaagaaga cggtatcatc ggtaaaggtg agctcgctcc aatcgaagat gctaaagcaa
    10621 tggttgaaac tggaattgac ttcttggcag ctggtatcgg taacatccac ggtccatacc
    10681 cagaaaactg ggaaggtctt gctcttgacc acttagaaaa attaacagca gctgtaccag
    10741 gtttcccaat cgtattgcac ggtggttctg gtattcctga cgatcaaatc aaagaagcaa
    10801 tccgtcttgg tgttgctaaa gttaatgtta acactgaaag tcaaatcgct ttctctaacg
    10861 caactcgtga atttgctcgt aactacgaag ctaacgaagc agaatatgat ggtaaaaaat
    10921 tatttgaccc acgtaaattc ttggctccag gtatgaaagc tgttcaaggt gctgttgaag
    10981 aacgtatcga tgttttcgga tcagctaaca aagcataatt tagagtatta aacaccctaa
    11041 agccctcttg agggcttttt tagatataat tgacacccta aaatggatac cagtttctgt
    11101 gaaataatct ttgattgtga acaaacaaag cataatttag agtattaaaa tctggaccct
    11161 gctatctatg gtaagtcttt ttgttattgt taaagaggtt aagcgtaatg ttaatatgtt
    11221 ttagctaaaa aatgtagtga aaaatgagta cgtagactat tgtaatagta attccgtaaa
    11281 attgttgaaa aagaaaatgg gaaatacctt gtcaaattaa gcaccctatg atagaataga
    11341 taggtatgtg gtgctagcac atccgtaaac atattctacg taggaggaaa tagatcaaaa
    11401 tggctaaagt atgttatttt acaggacgta aaaccgtttc tggaaacaac cgttcacacg
    11461 cgatgaacca aacaaaacgt acagttaaac caaaccttca aaaagttact atccttgttg
    11521 atggtaaacc taaaaaagtt tgggcttctg ctcgtgcgct taaatctggt aaagtagaac
    11581 gcatctaata gaaataagcc ttagggcttt ttttattagc ttatttctat ccataagcta
    11641 gtcaaggtaa taaacgctat cgttagcta
//
LOCUS       DAXWIQ010000048        11520 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000048 DAXWIQ010000000
VERSION     DAXWIQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11520
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.048"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            75..1298
                     /locus_tag="ACGEUI_001464"
     CDS             75..1298
                     /locus_tag="ACGEUI_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFF8166288.1"
                     /translation="MPSMKHILKSLSILLIGFLGGLIAIITFNNLYPHSPSKINSGKA
                     TTSNMVFNNTTNTTKAVKAVQNAVVSVINYQDNPSSSLSNPYTKLFGEGRSKENKDAE
                     LSIFSEGSGVIYRKDGNSAYVVTNNHVIDGAKRIEILMADGSKVVGELVGADTYSDLA
                     VVKISSDKIKTVAEFADSTKLNVGEVAIAIGSPLGTQYANSVTQGIVSSLSRTVTLKN
                     ENGETVSTNAIQTDAAINPGNSGGPLINIEGQVIGINSSKISSTPTGSNGNSGAVEGI
                     GFAIPSTDVIKIIKQLETNGEVIRPALGISMVNLNDLSTNALSQINIPTSVTGGIVVA
                     EVKEGMPASGKLAQYDVITEIDGKTVNSISDLQSSLYGHDINDTIKVTFYRGTTKKKA
                     DIKLTKTTQDLTKTQ"
     gene            1387..2163
                     /locus_tag="ACGEUI_001465"
     CDS             1387..2163
                     /locus_tag="ACGEUI_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFF8166289.1"
                     /translation="MTKELLIDLPIEDIVTNPYQPRIQFNQRELQDLATSIKSNGLIQ
                     PIIVRKSDIFGYELVAGERRLKASKMAGLKKVPAIIKKISTLESMQQAIVENLQRSNL
                     NAIEEAKAYQLLVEKKHMTHDEIAKYMGKSRPYISNTLRLLQLPAPIIKAIEEGKISA
                     GHARALLTLSDDKQQLYLTHKIQNEGLSVRQIEQLVTSTPSSKLSKKTKNIFATSLEK
                     QLAKSLGLSVNMKLTANHSGYLQISFSNDDELNRIINKLK"
     gene            2396..3751
                     /gene="dnaA"
                     /locus_tag="ACGEUI_001466"
     CDS             2396..3751
                     /gene="dnaA"
                     /locus_tag="ACGEUI_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFF8166290.1"
                     /translation="MTENEQIFWNRVLELAQSQLKQATYEFFVHDARLLKVDKHIATI
                     YLDQMKELFWEKNLKDVILTAGFEVYNAQISVDYVFEEDLMIEQNQTKINQKPKQQAL
                     NSLPTVTSDLNSKYSFENFIQGDENRWAVAASIAVANTPGTTYNPLFIWGGPGLGKTH
                     LLNAIGNSVLLENPNARIKYITAENFINEFVIHIRLDTMDELKEKFRNLDLLLIDDIQ
                     SLAKKTLSGTQEEFFNTFNALHNNNKQIVLTSDRTPDHLNDLEDRLVTRFKWGLTVNI
                     TPPDFETRVAILTNKIQEYNFIFPQDTIEYLAGQFDSNVRDLEGALKDISLVANFKQI
                     DTITVDIAAEAIRARKQDGPKMTVIPIEEIQAQVGKFYGVTVKEIKATKRTQNIVLAR
                     QVAMFLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMISQDESLRIEIETIKNKI
                     K"
     gene            3906..5042
                     /gene="dnaN"
                     /locus_tag="ACGEUI_001467"
     CDS             3906..5042
                     /gene="dnaN"
                     /locus_tag="ACGEUI_001467"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFF8166291.1"
                     /translation="MIQFSINRTLFIHALNTTKRAISTKNAIPILSSIKIEVTSTGVT
                     LTGSNGQISIENTIPVSNENAGLLITSPGAILLEASFFINIISSLPDISINVKEIEQH
                     QVVLTSGKSEITLKGKDVDQYPRLQEVSTENPLILKTKLLKSIIAETAFAASLQESRP
                     ILTGVHIVLSNHKDFKAVATDSHRMSQRLITLDNTSADFDVVIPSKSLREFSAVFTDD
                     IETVEVFFSPSQILFRSEHISFYTRLLEGNYPDTDRLLMTEFETEVVFNTQSLRHAME
                     RAFLISNATQNGTVKLEITQNHISAHVNSPEVGKVNEDLDIVSQSGSDLTISFNPTYL
                     IESLKAIKSETVKIHFLSPVRPFTLTPGDEEESFIQLITPVRTN"
     gene            5117..5314
                     /locus_tag="ACGEUI_001468"
     CDS             5117..5314
                     /locus_tag="ACGEUI_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HFF8166292.1"
                     /translation="MYQIGSFVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            5644..6759
                     /gene="ychF"
                     /locus_tag="ACGEUI_001469"
     CDS             5644..6759
                     /gene="ychF"
                     /locus_tag="ACGEUI_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFF8166293.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASRGEGLGNKFLANIREIDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEDEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ANPDGIDYVKQIRDFAATENAEVVVISARAEEEISELDDEDKEEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LMTYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            6829..7398
                     /gene="pth"
                     /locus_tag="ACGEUI_001470"
     CDS             6829..7398
                     /gene="pth"
                     /locus_tag="ACGEUI_001470"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFF8166294.1"
                     /translation="MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNF
                     KAQIGSTFINHEKVYFVKPTTFMNNSGIAVKALLTYYNIDITDLIVIYDDLDMEVSKL
                     RLRSKGSAGGHNGIKSIIAHIGTQEFNRIKVGIGRPLKGMTVINHVMGQFNTEDNIAI
                     SLTLDRVVNAVKFYLQENDFEKTMQKFNG"
     gene            7401..10904
                     /gene="mfd"
                     /locus_tag="ACGEUI_001471"
     CDS             7401..10904
                     /gene="mfd"
                     /locus_tag="ACGEUI_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFF8166295.1"
                     /translation="MDILELFSQNKKVQSWHSGLTTLGRQLVMGLSGSSKTLAIASAY
                     LDDQKKIVVVTSTQNEVEKLASDLSSLLDEELVFQFFADDVAAAEFIFASMDKALSRI
                     ETLQFLRNPKSQGVLIVSLSGLRILLPNPDVFTKSQIQLTVGEDYDSDTLTKQLMTIG
                     YQKVSQVISPGEFSRRGDILDIYEITQELPYRLEFFGDDIDSIRQFHPETQKSFEQLE
                     GIFINPASDLIFEVSDFQRGIEQLEKALQTAQDDKKSYLEDVLAVSKNGFKHKDIRKF
                     QSLFYEKEWSLLDYIPKGTPIFFDDFQKLVDKNARFDLEIANLLTEDLQQGKALSNLN
                     YFTDNYRELRHYKPATFFSNFHKGLGNIKFDQMHQLTQYAMQEFFNQFPLLIDEIKRY
                     QKNQTTVIVQVESQYAYERLEKSFQDYQFRLPLVSANQIVSRESQIVIGAISSGFYFA
                     DEKLALITEHEIYHKKIKRRARRSNISNAERLKDYNELAVGDYVVHNVHGIGRFLGIE
                     TIQIQGIHRDYVTIQYQNSDRISLPIDQISSLSKYVSADGKEPKINKLNDGRFQKTKQ
                     KVARQVEDIADDLLKLYAERSQQKGFSFSPDDDLQRAFDDDFAFVETEDQLRSIKEIK
                     ADMESMQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHYENFKA
                     RFENYPVEVDVLSRFRSKKEQAETLERVRKGQIDIIIGTHRLLSKDVVFSDLGLIVID
                     EEQRFGVKHKETLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLENNPGLVREAIIREMDRGGQIFYVYNKVDTIEKKVAELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFIENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIASKQGKTTVRQKGNTEINLQIDAYLPDDYIADERQK
                     IDIYKRIREIQSREDYLNLQDELMDRFGEYPDQVAYLLEIALLKHYMDNAFAELVERK
                     NNQVIVRFEVTSLTYFLTQDYFEALSKTHLKAKISEHQGKIDIVFDVRHQKDYRILEE
                     LMLFGERLSEIKIRKNNSVFK"
     gene            11066..11338
                     /locus_tag="ACGEUI_001472"
     CDS             11066..11338
                     /locus_tag="ACGEUI_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFF8166296.1"
                     /translation="MRLDKYLKVSRLIKRRSVAKEVADKGRIKVNGILAKSSTNIKLN
                     DHIEISFGNKLLTVRVIEIKDSTKKEDALKMYEIISETRITLNEEA"
     gene            11325..>11520
                     /locus_tag="ACGEUI_001473"
     CDS             11325..>11520
                     /locus_tag="ACGEUI_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFF8166297.1"
                     /translation="MKKPSIVQLNNHYIKKENLKKKFEEEESQKRNRFMGWILVSMMF
                     LFILPTYNLVKSYVDFEKQNQ"
BASE COUNT         4024 a         1806 c         2101 g         3589 t
ORIGIN      
        1 ttcttttttt tatttttaag gtataattaa gcaaagttct ataagctatg gacaaataat
       61 tcggaggaca aaacatgcca agtatgaaac atatcttaaa atccttaagt attttactaa
      121 ttggattttt aggaggatta atagcaatta ttacattcaa taatctctac ccacattctc
      181 catcaaaaat aaattcaggt aaggcaacaa ctagtaatat ggtttttaac aacacaacca
      241 atactactaa agctgtcaaa gctgttcaaa atgcagttgt atcagttatt aattatcaag
      301 ataacccttc ttcatccctt tctaaccctt atacaaaact ctttggagaa gggcgttcaa
      361 aagagaataa ggatgctgaa ttatctattt ttagtgaagg atctggggtc atttatcgaa
      421 aagatggcaa ctccgcttac gttgttacta ataaccatgt tatcgacgga gctaaacgaa
      481 ttgaaattct tatggcagac ggatctaaag ttgttggtga attagttgga gctgatactt
      541 attcggattt agctgttgtt aagatctctt cagataagat aaaaacagta gctgaatttg
      601 cagattctac aaaactaaat gttggagaag ttgctattgc tatcggcagc ccactaggaa
      661 cacaatacgc taattctgtt actcaaggaa tcgtctctag tttaagtcgt actgtaactt
      721 taaaaaacga gaatggtgag actgtctcaa caaatgctat tcagacagat gctgctatta
      781 accctggaaa ctctggtgga ccactaatta atattgaggg gcaagtaatc ggaattaatt
      841 caagtaaaat ttcttctacc ccaactggta gcaacggtaa tagtggtgct gttgaaggaa
      901 ttggttttgc tatcccatct actgatgtta ttaaaattat taaacaacta gaaactaatg
      961 gtgaagttat cagacctgct cttggaatat ccatggtcaa tttaaatgac ttatccacaa
     1021 atgctcttag tcaaattaat attccaacta gtgtaactgg tggtattgta gtagcagaag
     1081 ttaaggaagg aatgccggca tctggcaaac ttgctcagta cgatgtgatt acagaaattg
     1141 atggcaaaac agttaattca attagtgatt tacaaagtag tctatacgga cacgatatta
     1201 atgatactat taaagtaact ttttatagag gaactacaaa gaaaaaagca gatatcaaat
     1261 taacaaaaac tactcaagat ttgactaaaa cgcagtaaac aagctaccta aatactatta
     1321 cattttatca cataaaacag tccaaaggac tgttttatgt ttcttattct atccaggaac
     1381 tcccttatga caaaagaatt attgatagac ctaccaattg aagatattgt tacaaatccc
     1441 tatcagccaa ggatacaatt taatcaaaga gaactccaag acctagcaac ttctattaaa
     1501 tcaaacggcc tcattcaacc tattattgtt cgaaaatctg acatttttgg ctatgagtta
     1561 gttgctggag aacgacgact taaagcttca aaaatggctg gtctaaaaaa agttccagct
     1621 atcatcaaga agatctccac actcgagagc atgcaacaag ctatagttga aaatttacaa
     1681 cgttctaacc ttaacgctat cgaagaagct aaagcctatc agttattggt tgaaaaaaaa
     1741 cacatgactc acgatgagat tgctaaatat atgggaaaat caagacctta tattagcaat
     1801 accttacgtc tgttacaact cccagcacct atcattaaag caattgaaga aggaaaaatt
     1861 agtgcgggac acgcgcgtgc tcttttaact ttgagtgatg ataagcaaca actgtacctc
     1921 actcataaaa tacaaaatga aggcctaagt gttaggcaaa ttgagcaact ggttacttct
     1981 actccaagtt cgaagctatc taaaaaaact aaaaatattt ttgccacttc tttagagaaa
     2041 caattggcta aatcattggg actctctgtc aatatgaagc tgacagcaaa ccatagtggg
     2101 taccttcaga tatctttttc caatgatgat gaattaaaca gaattatcaa caagctaaag
     2161 tagcttgttg atattctgtt ttttcttttt tagttttcca catgaaaaat agttgaaaac
     2221 aatagcggtg tccccttaaa atggcttttc cacaggttgt ggagaaccca aattaacagt
     2281 gttaatttat tttccacagg ttgtggaaaa actaactatt atccatcgtt ctgtggaaaa
     2341 ctagaatagt ttatggtaga atagttctag aattatccac aagaaggaac ctagtatgac
     2401 tgaaaatgaa caaatttttt ggaacagggt cttggaatta gctcagagtc aattaaaaca
     2461 ggcaacttat gaattttttg ttcatgatgc ccgtctatta aaggtcgata agcatattgc
     2521 aactatttac ttagatcaaa tgaaagagct cttttgggaa aaaaatctta aagatgttat
     2581 tcttactgct ggttttgaag tttataacgc tcaaatttct gttgactatg ttttcgaaga
     2641 agacctaatg attgagcaaa atcagaccaa aatcaaccaa aaacctaagc agcaagcctt
     2701 aaattctttg cctactgtta cttcagattt aaactcgaaa tatagttttg aaaactttat
     2761 tcaaggagat gaaaatcgtt gggctgttgc tgcttcaata gcagtagcta atactcctgg
     2821 aactacctat aatcctttgt ttatttgggg tggccctggg cttggaaaaa cccatttatt
     2881 aaatgctatt ggtaattctg tactattaga aaatccaaat gctcgaatta aatatatcac
     2941 agctgaaaac tttattaatg agtttgttat ccatattcgc cttgatacca tggatgaatt
     3001 gaaagaaaaa tttcgtaatt tagatttact ccttattgat gatatccaat ctttagctaa
     3061 aaaaacgctc tctggaacac aagaagagtt ctttaatact tttaatgcac ttcataataa
     3121 taacaaacaa attgtcctaa caagcgaccg tacaccagat catctcaatg atttagaaga
     3181 tcgattagtt actcgtttta aatggggatt aacagtcaat atcacacctc ctgattttga
     3241 aacacgagtg gctattttga caaataaaat tcaagaatat aactttattt ttcctcaaga
     3301 taccattgag tatttggctg gtcaatttga ttctaatgtc agagatttag aaggtgcctt
     3361 aaaagatatt agtctggttg ctaatttcaa acaaattgac acgattactg ttgacattgc
     3421 tgccgaagct attcgcgcca gaaagcaaga tggacctaaa atgacagtta ttcccatcga
     3481 agaaattcaa gcgcaagttg gaaaatttta cggtgttacc gtcaaagaaa ttaaagctac
     3541 taaacgaaca caaaatattg ttttagcaag acaagtagct atgtttttag cacgtgaaat
     3601 gacagataac agtcttccta aaattggaaa agaatttggt ggcagagacc attcaacagt
     3661 actccatgcc tataataaaa tcaaaaacat gatcagccag gacgaaagcc ttaggatcga
     3721 aattgaaacc ataaaaaaca aaattaaata acatgtggaa aagaatatct tttatgaaat
     3781 agttatccac aagttgtgaa catccattta gtcttggatt ctctcgttta tttagagtta
     3841 tccactatat acacaagacc tactactact acttattatt atacttatta aataaaggag
     3901 ttctcatgat tcaattttca attaatcgca cattatttat tcatgcttta aatacaacta
     3961 aacgtgctat tagcactaaa aatgccattc ctattctttc atcaataaaa attgaagtca
     4021 cttctacagg agtaacttta acagggtcta acggtcaaat atcaattgaa aacactattc
     4081 ctgtaagtaa tgaaaatgct ggtttgctaa ttacctctcc aggagctatt ttattagaag
     4141 ctagtttttt tattaatatt atttcaagtt tgccagatat tagtataaat gttaaagaaa
     4201 ttgaacaaca ccaagttgtt ttaaccagtg gtaaatcaga gattacctta aaaggaaaag
     4261 atgttgacca gtatcctcgt ctacaagaag tatcaacaga aaatcctttg attttaaaaa
     4321 caaaattatt gaagtctatt attgctgaaa cagcttttgc agccagttta caagaaagtc
     4381 gtcctatttt aacaggagtt catattgtat taagtaatca taaagatttt aaagcagtag
     4441 cgactgactc tcatcgtatg agccaacgtt taatcacttt ggacaatact tcagcagatt
     4501 ttgatgtagt tattccaagt aaatctttga gagaattttc agcagtattt acagatgata
     4561 ttgagaccgt tgaggtattt ttctcaccaa gccaaatctt gttcagaagt gaacacattt
     4621 ctttttatac acgcctctta gaaggaaatt atcccgatac agaccgttta ttaatgacag
     4681 aatttgagac ggaggttgtt ttcaataccc aatcccttcg ccacgctatg gaacgtgcct
     4741 tcttgatttc taatgctact caaaatggta ctgttaagct tgagattact caaaatcata
     4801 tttcagctca tgttaactca cctgaggttg gtaaggtaaa cgaggattta gatattgtta
     4861 gtcagtctgg tagtgattta actatcagct tcaatccaac ttaccttatt gagtctttaa
     4921 aagctattaa aagtgaaaca gtaaaaattc atttcttatc accagttcga ccattcaccc
     4981 taacaccagg cgatgaggaa gaaagtttta tccaattaat tacaccagta cgaacaaact
     5041 aagtaagaaa aagctccctt ttaggagttt ttttgttatt ataaatatta acgataatat
     5101 aagtggagaa aagggaatgt atcaaattgg atcatttgtt gaaatgaaaa aacctcatgc
     5161 ttgtgtaatt aaagaaactg gtaaaaaggc taatcaatgg aaggtactga gagtaggagc
     5221 tgatattaaa attcagtgta ctaactgtca gcatgtgatt atgatgagtc gttatgattt
     5281 tgagcgaaaa ttaaaaaaag tactgcaacc atgaaagtcc ttttaaataa ataattctag
     5341 ctatatttgc aaccaatact ttgctaaaaa attgctagta tgccgttgga aaattagcta
     5401 ttctaatgtt atcgaaagaa gaaagatggc cattgacaat gacgaaagtt gtagcacagt
     5461 tacttagttt ctcggcaaac tatatcaaaa tgagaataat acaccaaact ttgataattt
     5521 aaatgaaggt gaaagagtgg cataatgata agcaaatctg aaattttctt cttattcata
     5581 ccgctttttc ttctattttt tggtataata atcttgattg aaatttgaat ggagattcgc
     5641 taaatggctt taacagcagg tattgtaggc ttaccaaatg ttggtaaatc aaccttattt
     5701 aacgcaatta caaaagcagg agcagaagct gctaattatc cttttgcgac tattgatccc
     5761 aatgttggta tggtagaggt tccagatgag cgtctgcaaa aattgacaga attgattaca
     5821 cctaaaaaaa cagttccgac aacctttgaa tttactgata ttgcaggtat cgttaaaggt
     5881 gcttctagag gggaaggtct aggaaataaa tttttagcta atattcgtga aatcgatgct
     5941 attgttcatg tggtacgtgc ttttgatgat gaaaatgtca tgcgtgaaca aggtcgtgag
     6001 gatgctttcg ttgatcctat agcagatatt gacactatta atcttgaatt aatcttagct
     6061 gatttagagt caatcaataa acgttatgcg cgtgttgaaa aaatggcacg aactcaaaaa
     6121 gataaagaat cagtagcaga gtttaatgtt ttgcaaaaga ttaaacctgt tttggaagat
     6181 gggaaatcag ctaggacaat tgagtttaca gaagatgaag caaaagttgt taaaggtctc
     6241 tttttattaa caactaaacc tgttttatat gtagctaatg tcgatgaaga taaagttgct
     6301 aatccagatg gtattgatta tgtcaaacaa attcgtgact ttgcagctac tgaaaatgct
     6361 gaagtagttg ttatctcagc gcgtgcagaa gaagaaattt cagagcttga cgatgaggat
     6421 aaagaagaat ttttggaagc tatcggtctt actgaatcag gcgttgataa attaaccaga
     6481 gcagcttatc atctcttagg ccttggaacc tattttacag caggtgaaaa agaggttcgt
     6541 gcttggacgt ttaagcgtgg tataaaagct ccacaagctg ctggtattat ccattcagat
     6601 tttgaaagag gttttattcg tgcagtaacc atgtcttatg atgatctaat gacctacggt
     6661 tcagaaaaag ccgtcaaaga agctggacgc ttgcgtgaag aaggaaaaga atatgttgtc
     6721 caagatgggg acatcatgga attcagattt aatgtgtaat tatattaaaa caatatcaga
     6781 aggttggaag aacattccag cccttttggc attttagaaa gagaaaatat ggtaaaaatg
     6841 attgttggtc tgggaaatcc aggctctaaa tatgaaaaaa caaagcacaa tattggtttt
     6901 atggctattg acaatattgt caagaacctt gacgttacct ttacagatga taaaaatttt
     6961 aaagcacaga taggaagtac ttttattaat catgaaaaag tttactttgt gaaacctact
     7021 acttttatga ataatagcgg catagcagta aaagcattac taacctacta taatattgac
     7081 ataacagatt taattgttat ctatgatgat ttagacatgg aagtcagtaa attacgttta
     7141 cgtagtaagg gttcagcagg aggacataat ggcattaagt caatcattgc ccacattgga
     7201 actcaggaat ttaaccgaat caaagttggt attggacgac ctttaaaagg tatgactgtt
     7261 attaaccatg tgatgggcca attcaatacc gaagataata ttgctatttc gttaactctt
     7321 gacagagttg tcaatgctgt caagttttat ttacaagaaa atgattttga aaaaacaatg
     7381 cagaaattta atggataatc atggatattt tagaattatt tagtcagaat aagaaagtcc
     7441 aatcctggca ctctggatta accaccttag gaagacaact ggtaatgggg ttatcgggtt
     7501 caagtaaaac attggctata gcttccgctt atttagatga tcaaaaaaaa atagttgtgg
     7561 ttacatcaac tcaaaatgag gttgaaaaat tagccagcga tttatctagt ttacttgatg
     7621 aagaacttgt tttccaattt tttgcagacg atgtggctgc agcggaattt atctttgcgt
     7681 caatggataa agctctatca agaatagaaa ccctgcaatt tttaaggaat cctaaatctc
     7741 agggcgtttt aattgttagt ttatcaggct taagaatttt attgccaaac ccagatgttt
     7801 ttacaaagag tcagattcaa ctaacagttg gagaagatta tgatagtgat actcttacta
     7861 aacaactgat gacaattggc tatcagaagg tctcacaggt tattagtcca ggagaattta
     7921 gccgtcgagg ggatatttta gatatctatg agattacaca agaattgcct tatcgattgg
     7981 aattttttgg cgatgatatt gatagtatta ggcaatttca tccagaaact caaaaatctt
     8041 ttgaacaact agaaggtatt tttattaatc cagcaagtga tcttattttt gaggttagtg
     8101 attttcaacg tggcattgag caattagaaa aggctctaca aacagcacaa gatgataaaa
     8161 aatcttattt agaagatgta ttagctgttt caaaaaacgg ttttaaacat aaggatatcc
     8221 gtaaatttca atcattattt tatgaaaaag agtggtcatt attagattat attcctaagg
     8281 gaacgccaat cttttttgat gattttcaaa aactagttga taaaaatgca agatttgatt
     8341 tagagattgc taatctcttg acagaagatt tacagcaagg aaaggctctt tccaatctta
     8401 actactttac agataattat cgagagctta ggcactataa gccagcgacc ttcttttcaa
     8461 attttcataa gggacttgga aatatcaaat ttgatcagat gcatcagcta actcagtatg
     8521 ccatgcagga attttttaat caatttcctt tgttgattga tgagattaaa cggtatcaaa
     8581 aaaatcaaac aacggttatt gtacaggtag agtctcagta tgcttacgaa cgacttgaaa
     8641 aatcttttca agattaccaa tttcgccttc ctttagtgag tgctaatcaa attgtttcac
     8701 gtgaatcaca aattgtaatt ggagctatct ccagtggttt ttattttgct gatgaaaagt
     8761 tagcgttaat cacagagcat gaaatttatc ataaaaagat caaacgacgc gctagacgat
     8821 ctaatattag caatgctgag cgtttgaaag attacaatga gttagcagta ggtgattacg
     8881 tggttcataa tgtccatggt atcggtcgct ttcttggaat tgaaacgatt cagattcagg
     8941 gaatccatcg agattatgtt actattcaat atcaaaattc agaccgtatt tctcttccaa
     9001 ttgaccaaat tagtagctta tcaaagtacg tttctgctga tggaaaagaa cctaaaatta
     9061 ataaactcaa tgatggtcgt tttcaaaaga caaagcaaaa ggtcgctaga caagtagagg
     9121 atattgctga tgaccttcta aaattatatg ctgaaagaag tcagcaaaaa ggattttcat
     9181 tttcaccaga tgatgacttg cagcgcgctt ttgatgatga ttttgctttt gtagaaacag
     9241 aagatcaact taggtctata aaggaaatta aagctgatat ggagagcatg cagcctatgg
     9301 atcgtctttt agtaggcgat gtaggatttg gtaagacaga agtagctatg agggcagcct
     9361 ttaaagcggt gaatgatcac aaacaagtag ctgtcttagt tccaaccaca gtcttggccc
     9421 agcagcatta tgaaaacttc aaagcacgct ttgaaaatta ccctgttgag gttgacgtct
     9481 taagtcgttt ccgtagtaaa aaagagcaag ctgaaacact agaacgtgta cgaaaaggtc
     9541 aaattgacat tattatcgga acccatcgac tcctatcaaa agatgtggtc ttttctgatt
     9601 taggattgat tgtgattgat gaggaacaac ggtttggtgt taagcataaa gaaactttaa
     9661 aggaattaaa aactaaggtt gacgtcttaa ccttaacagc tactccgatt cctagaacct
     9721 tacacatgtc tatgttaggt atccgagatt tatcggttat tgagacccca ccaaccaatc
     9781 gttatcctgt tcaaacctat gttttggaaa ataatccagg tctcgttaga gaagctatca
     9841 ttcgtgaaat ggatcgcgga ggacaaattt tttacgttta caataaagtt gacactattg
     9901 aaaagaaagt tgcagagcta caagaattag tcccagaagc ttctataggt tttgttcatg
     9961 ggcaaatgag tgaaattcaa cttgaaaata ccttgattga ctttataaat ggtgattatg
    10021 atgtccttgt ggctacaaca atcatcgaaa caggagttga catttctaat gtaaacactt
    10081 tgtttattga gaatgctgat catatgggat tgtcaacttt atatcaacta cgagggcgcg
    10141 tcggaagaag taatcgtatt gcttatgctt acctgatgta tcgtcctgat aaggtcctaa
    10201 cagaggtttc tgaaaagcgt ttagaggcta ttaaaggctt tactgaatta ggttcaggct
    10261 tcaagattgc tatgcgagat ttgtctatac gaggagcagg taatatttta ggggcttctc
    10321 agagtggctt tattgattcg gtcggttttg aaatgtattc tcagttattg gaacaggcta
    10381 ttgctagcaa gcagggaaaa acgactgttc gtcaaaaagg caatactgaa atcaatcttc
    10441 agattgatgc ctatttacca gatgattata ttgcagatga gcgccaaaaa attgacattt
    10501 acaagcgtat tcgagaaatt caatcaagag aagattatct caatttgcaa gatgagctta
    10561 tggatcgttt tggagagtat cctgatcaag ttgcctattt gttagagata gctctgctaa
    10621 aacattatat ggacaatgcc tttgcggaat tagttgaacg caaaaataat caggtcattg
    10681 ttcgatttga agtaacttct ttaacttatt tcttgactca ggattatttc gaagccttat
    10741 cgaaaaccca tcttaaagct aaaattagtg aacatcaggg gaaaatcgat atcgtctttg
    10801 acgttcgcca tcaaaaagat tatagaattt tagaagaatt gatgttattt ggagaaaggc
    10861 ttagtgagat aaaaatcaga aaaaacaatt cagtttttaa ataataattt gaaacaactt
    10921 ttagactgaa tagtattaat gacaatggaa cgaactagct ctttatactt ttgtcaaaaa
    10981 tctaagatct ttgctgcgaa attaggatct tttctaccat ttaaaaaata aaaatgataa
    11041 aatgaagagg attgtaggag aaattatgag actagataaa tatctaaagg tatcgcgcct
    11101 tattaaacgt cgttcagtag caaaagaagt tgcggataag ggacgaatta aagttaatgg
    11161 gatacttgct aaaagttcaa cgaatataaa actaaatgat cacattgaaa ttagttttgg
    11221 aaataaatta ctgacagtaa gagtcattga aataaaagat agtacaaaaa aagaagatgc
    11281 tcttaagatg tatgagataa tcagtgaaac aaggataaca ttaaatgaag aagcctagta
    11341 ttgttcaatt aaataatcat tatattaaga aagagaatct caaaaaaaaa tttgaagaag
    11401 aagaatctca aaaaagaaat cgttttatgg gatggatcct tgtaagtatg atgtttttat
    11461 ttattttgcc aacttataat cttgtcaaaa gttatgttga ttttgaaaag caaaatcaac
//
LOCUS       DAXWIQ010000049        11001 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000049 DAXWIQ010000000
VERSION     DAXWIQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11001
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.049"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(<1..730)
                     /locus_tag="ACGEUI_001474"
     rRNA            complement(<1..730)
                     /locus_tag="ACGEUI_001474"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1116..2189)
                     /locus_tag="ACGEUI_001475"
     CDS             complement(1116..2189)
                     /locus_tag="ACGEUI_001475"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFF8166298.1"
                     /translation="MKILVAIDSFKGSVTSPELNTSVAQALLSVDKQLVIETRAIADG
                     GEGSLVALSQTVAGRWHQVKTIDLLRRPIKVAYYRHAKQAFIESASIIGIDKITSNSV
                     TYAQATSYGLGLAVKDAIQKGATQIEIMLGGTGTSDGGKGFLESLNYDFMTGRSYLDT
                     LASPVTLLGLTDVTNPYHGPQGFAAVFGPQKGGSLSQIEETDQIASNFAKKVFCQTTI
                     DLQTIPGSGAAGGLGGAIVLLGGTLTSGFSRIAELLNLDNSLQSCDLVITGEGCLDTQ
                     SQSGKVPVAIARMAKKYQVPTIALCGSVKIETGLAAEDFLAVFSIQQQPISLEAAIDK
                     TTTLSNIKILAANLMLLIAQFNK"
     gene            2540..5518
                     /locus_tag="ACGEUI_001476"
     CDS             2540..5518
                     /locus_tag="ACGEUI_001476"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFF8166299.1"
                     /translation="MGNQTKTELELEKELIHLLETGESQWTYRKELKTEDALWDNFFK
                     ILAQNNTQYLNEEPLTASEKEQIKNQLNFVNYYEAAKWLAGENGIAKVQVQREDAKLG
                     TIRLEVVKADNVAGGTSVYEIANQVAFSGSRDRRGDVTLLINGLPMIQIELKSQNHQC
                     IEAFNQVKKYDKEGQFRGIFSTLQMFVVSNKTDTRYIAAAKENKLNPNFLTQWVDQNN
                     KPQKDLFAFAKEVLSIPRAHQMVMTYSVIDDDKKALILLRPYQIHAIEAVAEASRHRK
                     SGYIWHTTGSGKTLTSYKVARNILQIPAVEKSIFVIDRKDLDNQTASAFQSYAQNDIF
                     DVDETEDTRQLIKNLESSDRRVVVTTIQKLNAMISQMESYDTPKFKKLKERLAHLNVV
                     FVVDECHRAVTPERQRYLTNTFRNSRWYGFTGTPIFVENKRAQLGDLAQTTEQQYGKC
                     LHQYTVKEAIHDKAVLGFQVEYKTTIPDMPEDSIPEEAYDHEEHMLAVLDSIINQSRK
                     KLGFNNGIGQTFEGLLTVKSIARAQAYYDLMKKVKAGETDLVISKKVKEKLPDFPKVA
                     ITYSITENDNASISRQDKMTKNLEDYNHLFGTNFTIDNLQGYNRDLNDRLARKKDKFK
                     DRHEQLDLVIVVDRLLTGFDAPCLSTIFIDRQPMKPQHIIQAFSRTNRIFESRKHYGQ
                     VVTFQTPLRFKEAVDKALSLYSNGGENDVLAPSWEEEKARFFEKVTVLKNIVPDPDAF
                     PTIESAQTAFLKQYAKAFQAFDKLFASVQVYSDFNETLLSEVGLSDEVIDTYKGTYQN
                     VIAEIRKRREDDEAIPEINIDYELESVQMDDINYHYILTLIQAFVDQEQEALQERLND
                     NPMDQYIQDLAKSNPAMADSLAELWQDIQKEPKAYEGKSIVYELDNLIGDKIQRAIKH
                     FADQWKADPDKLAFVATNYHSANSTKQVGMSTLKESLDYQAYKEKQGDSAMNKLKYKS
                     QFERELVQFIRDQIQPLKGH"
     gene            5531..6730
                     /locus_tag="ACGEUI_001477"
     CDS             5531..6730
                     /locus_tag="ACGEUI_001477"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFF8166300.1"
                     /translation="MTKSKQPQYRFDGFEGEWEEKELGDIVQITMGQSPSSQNYTTNP
                     SDYILVQGNADIKNGYVFPRVWTTQITKQADKGDIILSVRAPVGDVGKTNYHVIIGRG
                     VAAIKGNEFIFQILKYLKEIGYWKRISTGSTFDSISSSDIKYAKIQIPSLPEQEAIGE
                     LFQMVDQLIQLQDQKLATLKEQKQTFLRKMFPAQGQKVPEIRLQGFKGEWEEKKLREV
                     STHRSGTAIEKYFDSEGEFKVISIGSYGTNNLYVDQNIRAVSNELTNSKLVASGELTM
                     VLNDKTANGAIIGRCLLITENNKYVVNQRTEIIRPDINISSYYLFHYLNGEFRNGIIK
                     IAQGGTQIYVNYSSVEQLKINIPTLKEQEAIGNFFQTLDQQIAQSEEKLTELKALKQT
                     LLNRLFV"
     gene            6743..8323
                     /locus_tag="ACGEUI_001478"
     CDS             6743..8323
                     /locus_tag="ACGEUI_001478"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFF8166301.1"
                     /translation="MAEKTTSLRQALWHSADQLRGQMDANDYKNYLLGLIFYKHLSDK
                     LLLAVCDNLEKHFNTFTEAQKIFEDAYQDEGLKDDLISVVTGDLGYFIEPTLTFEKLI
                     QDVYHNTFQLESLAQGFRDIEQSGEDFENLFEDIDLYSKKLGSTPQKQNQTISNVMKT
                     LNEIDFEAVDGDTLGDAYEYLIGEFASESGKKAGEFYTPQAVSHLMTQIVFLGREDQK
                     GMTLYDPAMGSGSLLLNAKKYSNQSDTVSYYGQEINTSTYNLARMNMMLHGVAIENQH
                     LSNADTLDADWPTDEPINFDGVLMNPPYSLKWSATAGFLTDPRFSSYGVLAPKSKADF
                     AFLLHGFYHLKNTGTMAIVLPHGVLFRGAAEGKIRQKLLEQGAIDTIIGLPSNIFYNT
                     SIPTTIIILKKNRTNKDVFFIDASKEFDKGKNQNTMTDNHIKKILDAYKSRDNSDKFS
                     YLASFDEIIENDYNLNIPRYVDTFEEVPVKPLPELAKQLSDIDQEIAKTNAKLDQLMK
                     QLVGTTKEAQDELDTFRN"
     gene            complement(8533..8733)
                     /locus_tag="ACGEUI_001479"
     CDS             complement(8533..8733)
                     /locus_tag="ACGEUI_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166302.1"
                     /translation="MPDMKSFLNLVKQKLFKPGLKKLVKLHNSQNVNICLYINDWNYF
                     MVRTMVFVEGSKVVLKISLNRV"
     gene            complement(8876..9481)
                     /locus_tag="ACGEUI_001480"
     CDS             complement(8876..9481)
                     /locus_tag="ACGEUI_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8166303.1"
                     /translation="MKILLIDDHRLFAKSIQLLFQQYDEVDVIDTITSHFNDVTIDLS
                     KYDIILLDINLANISKENGLEIAKELIQSTPHLKVVMLTGYVKSIYRERAKKVGAYGF
                     VDKNIDPKQLISILKKVDSGKKYFEQIESQDYVESLTDQEIAILNLSKKGFSIKEIEE
                     TLQISRRTVFNHLTHIYSKLLVNNKQEAIYKAEQLGYFMDF"
     gene            complement(9462..>11001)
                     /locus_tag="ACGEUI_001481"
     CDS             complement(9462..>11001)
                     /locus_tag="ACGEUI_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166304.1"
                     /translation="KSKFFGTAIILLQQLIALLILVYNRENLSLLFSEKVALVMTLID
                     TAFIWLATILRQKQGDIFKRIISIISLTIWQYLVSVLTRGTPLFLSSGLQIILLYCYT
                     VEITNLILYGHKQFKDKLDKGLLIIIFVSITSLFVNRILFNFLFLMVFTILHLYPLLV
                     IVLYYRSFRQQISVVRRSLILFSLLLLVILGSELYGEMLDVNQAFNNLGWYLFPLIMS
                     VIYYFKTIHDKLSFVTQRWLGDYKARIELLFLLLILCWIVLMKVLVKDFLLFFIVVDA
                     STLFCLVVISCIFYYLENSKQNFDYENRRLNYFMKSEENMRVEFSNYLHDDVLQNIIA
                     IKNLFSLENSNITHGFIVNELNDLVSGIRDEMDTYHPIVPANQTMKENIQSLFDDIVK
                     SRKSNTLLYFNCSDNMVVPSPYGDIVYRFIKELINNAIKYGDGKDIRLSLTIQSDIII
                     IEESNQVVEKVHSINYGRGLKSFQETLAAFDGDLELQMDTKQFTIRILLPIDWKLCYE
                     DFIN"
BASE COUNT         3640 a         2161 c         1855 g         3345 t
ORIGIN      
        1 caccggtgtt cctccatata tctacgcatt tcaccgctac acatggaatt ccactctccc
       61 cttctgcact caagttctcc agtttccaaa gcgtacattg gttgagccaa tgcctttaac
      121 ttcagactta aaaaaccgcc tgcgctcgct ttacgcccaa taaatccgga caacgctcgg
      181 gacctacgta ttaccgcggc tgctggcacg tagttagccg tccctttctg gttagttacc
      241 gtcacttggt ggattttcca ctcccaccat cattcttctc taacaacaga gctttacgat
      301 ccgaaaacct tcttcactca cgcggcgttg ctcggtcagg gttgccccca ttgccgaaga
      361 ttccctactg ctgcctcccg taggagtctg ggccgtgtct cagtcccagt gtggccgatc
      421 accctctcag gtcggctatg tatcgtcgcc ttggtgagcc tttacctcac caactagcta
      481 atacaacgca ggtccatctc atagtggagc aattgcccct tttaaattac taacatgcgt
      541 tagtctctct tatgcggtat tagctatcgt ttccaatagt tatcccccgc tatgaggtag
      601 gttacctacg cgttactcac ccgttcgcaa ctccttgaac cggtgcaagc accagttctc
      661 agcgttctac ttgcatgtat taggcacgcc gccagcgttc gtcctgagcc aggatcaaac
      721 tctctttaat ttgcgtttga acttactcgt tccttctgtc gctgacagat ttatttctcg
      781 tttcttgacg ggtaatatgt ctccatatca ccctcacgtt tggttcgtct tattcagttc
      841 tcaaaggtct taacgtctcc taagagacaa cttctttatt ctatcaggtt tctcatagcc
      901 tgtcaactac tttttcatct tttttcaata agtgtgttac aggaacctct ctgacagctt
      961 tattagtata cgatatcttt actgtcattg tcaactcttt tttacaaaat gtttctgttt
     1021 ttcatactat gctaatgagt tagctcgtgc gtctccttct tcataggtta cttttaacca
     1081 ccaaatgaat gctacacaat cgaaaataga tgagtttact tattaaactg agctatcaat
     1141 agcataaggt tagctgctaa tattttgata ttagaaagtg ttgttgtttt atctatcgct
     1201 gcttctaagg aaattggctg ctgttgtatt gaaaaaacag ccaaaaagtc ttctgctgct
     1261 agtcctgtct caatctttac agaaccacat agagcaatcg ttggaacttg atatttttta
     1321 gccattcttg caatcgcaac aggtaccttg ccagattgtg attgtgtatc caggcatcct
     1381 tctccagtaa taactaaatc acaagactga agggaattat ccagatttag tagctctgca
     1441 atcctgctaa aaccactagt taaggtgcct cctaatagga caatggcacc tcctaatcct
     1501 ccagctgctc cacttcctgg tattgtctgt aaatcaatgg ttgtttggca aaaaactttt
     1561 ttagcaaagt tacttgcaat ttgatcagtt tcttcaattt gactaagact cccacctttt
     1621 tgagggccaa agaccgcagc aaatccttga gggccatgat atggattggt cacatcagtc
     1681 agccctaata gcgtaacagg tgatgctaaa gtatcaagat aagatcttcc tgtcatgaaa
     1741 tcatagttta aactttccaa aaaccctttg ccaccatcag aagtgcctgt gcctcctagc
     1801 atgatttcga tttgagtagc tcctttttga atggcatctt taactgccaa acccaaaccg
     1861 taggaagtgg cttgtgcata ggttactgaa ttggaggtga tcttatcaat accaataata
     1921 ctcgcagatt caataaaagc ttgctttgcg tgacgataat aagcgacttt aatcgggcgt
     1981 cttaatagat caatggtttt tacttgatgc cacctccccg ccacggtttg tgagagggct
     2041 actaaacttc cttctcctcc atcagcgata gctcttgttt caataactaa ctgcttatcg
     2101 acagaaagaa gggcttgagc tacactagtg ttaagttctg gagacgttac cgatccctta
     2161 aaagaatcta tggctaccaa aattttcata tcttcaataa attccttttc tgtatattgt
     2221 agcagattat catcctttaa aaccctatta atagtcaagc tagatctgtc ttaagttttt
     2281 tatctatttt gagaactcga cctttttact agtgttgtct tattactttc ttcaccttaa
     2341 attattgtca agcaaactca aaaatacaag aactttctcg attgcattta catagtcata
     2401 gctttgttta gctgctagga tcttaagcat tcaactaatc aaaaaaaaga ctgtttggaa
     2461 ggtttcaata gcttcgccat tctaaaaagg atccgccaat gatataatat tactatatca
     2521 tgtcgaatgg ggattaaaaa tgggaaatca aactaaaacg gaacttgaac ttgaaaaaga
     2581 gttgatccat ctcttagaaa caggtgaaag tcaatggaca tatcgtaaag aattaaaaac
     2641 tgaagatgct ttatgggata atttctttaa gatcttagcg caaaataaca cgcaatatct
     2701 gaacgaggag cctttaacag ctagtgaaaa agagcagatt aagaatcaac ttaactttgt
     2761 caattactat gaagcggcta aatggttagc tggtgaaaac ggcattgcta aagtacaagt
     2821 gcaacgcgaa gatgcaaaac taggaacgat tcgtttagaa gtcgttaaag cagacaatgt
     2881 ggctggtgga acttctgttt atgagattgc caatcaagtt gctttctctg gttctcgtga
     2941 tagacgaggt gatgtgacac ttctcatcaa tggcttacct atgatccaaa ttgaattaaa
     3001 atctcagaat catcaatgca ttgaagcttt taaccaagtc aaaaagtatg ataaagaagg
     3061 ccagtttaga ggtatctttt caaccttaca aatgtttgtg gtctcaaata aaacagatac
     3121 aaggtatatc gctgcagcta aagaaaataa attaaaccct aacttcttga cccaatgggt
     3181 agatcaaaac aacaaaccac aaaaagatct ctttgccttt gccaaagaag tcctatctat
     3241 ccctcgtgct caccaaatgg tcatgactta ctctgtcatt gatgatgata aaaaagcact
     3301 aattctcctt cgtccttatc agattcatgc aatcgaagcc gtcgctgaag cgagtcgtca
     3361 ccgcaagtct ggctatatct ggcacacaac aggttctgga aaaaccttaa cttcctataa
     3421 agtggctcgc aatatccttc aaataccggc cgttgaaaaa tccatctttg tcattgaccg
     3481 aaaggattta gataatcaga ccgcttctgc ttttcaatcc tatgcgcaaa atgatatctt
     3541 tgatgtcgat gagacagaag atacgagaca actcatcaaa aatttagaat ccagcgatcg
     3601 tcgtgttgtg gtcaccacca ttcaaaaact caatgccatg atcagtcaga tggaaagtta
     3661 tgacactcca aaattcaaga aactaaaaga gcgtcttgca cacttaaatg ttgtctttgt
     3721 tgtggacgaa tgccaccgtg ctgtcacgcc agaacgtcaa cgttatctca cgaatacttt
     3781 tcgtaacagt cgctggtatg gctttaccgg cactcctatc tttgtggaaa ataagcgtgc
     3841 tcaattaggt gatttagccc aaacaacgga acagcaatac ggcaaatgtc tccatcaata
     3901 caccgttaaa gaagccattc atgataaagc ggttctaggc tttcaagttg agtataagac
     3961 aaccattcct gatatgcctg aagacagtat tccggaagaa gcttatgatc atgaagaaca
     4021 tatgttagct gtcttagata gtatcattaa tcaatcacgc aagaaacttg gttttaataa
     4081 tggaattggt cagacattcg aaggtttact aaccgttaag agtattgctc gtgcccaagc
     4141 ctattatgat ctgatgaaga aagttaaggc tggtgaaacg gatttagtca tttctaagaa
     4201 ggtcaaagaa aaactaccag actttccaaa ggtagctatt acttattcta tcactgaaaa
     4261 tgacaatgcg tctatcagcc gacaagataa aatgaccaaa aatctagaag actataatca
     4321 cctctttggc actaacttta ccatcgataa cttacaaggc tataacagag atttaaatga
     4381 tcgcttagcc cgtaaaaaag ataaattcaa agaccgtcat gaacaattag acctcgtgat
     4441 tgtggttgac cgtctcttaa caggttttga tgcgccttgt ttatcaacga tctttattga
     4501 tcgccagcct atgaaacctc agcacattat tcaagctttc tcacgaacca accgtatctt
     4561 tgaaagtaga aaacattacg gtcaggttgt gactttccaa acaccacttc gttttaaaga
     4621 agctgtcgac aaagcgctta gcctctactc taatgggggt gaaaacgacg ttttggcccc
     4681 ttcttgggaa gaagaaaaag caagattctt tgaaaaagta acagtattaa aaaatatagt
     4741 tcctgatcca gacgctttcc caactattga gagcgcacag acagcttttc tcaaacaata
     4801 cgccaaagcc tttcaagcgt ttgataaact ctttgcatct gtacaggtct atagtgattt
     4861 taatgaaaca ctgttgagtg aggttgggtt atcagatgaa gtgattgata cttataaagg
     4921 cacttaccaa aatgtcattg cagaaatacg aaagcgaaga gaagatgacg aagccatccc
     4981 agagatcaat atcgactatg agctagaaag tgttcagatg gatgatatca actatcacta
     5041 tatcttgacc ttaattcaag cctttgtcga tcaagaacaa gaagctcttc aagaaaggct
     5101 caatgacaat cctatggatc aatacatcca agatttggca aaatcaaacc ctgctatggc
     5161 tgactcacta gctgagttat ggcaagatat ccaaaaagaa cccaaagcct atgaagggaa
     5221 aagcattgtt tatgagttag ataacctcat tggtgataag atccaaaggg ccatcaaaca
     5281 ctttgcggac caatggaagg cagatcctga caaattagcc tttgtggcga ctaattatca
     5341 tagtgctaac tctaccaaac aagtgggcat gtcaacctta aaagaatccc tagactacca
     5401 agcctataaa gaaaaacaag gtgattctgc catgaataaa ctcaaataca agagccaatt
     5461 tgaacgggag ttagttcaat tcattagaga tcaaatccaa cccttaaaag ggcactagga
     5521 aaggaaagag atgacaaaat ctaaacagcc tcaatatcgc tttgacggtt ttgaagggga
     5581 gtgggaagaa aaagaactag gggatattgt tcaaattaca atgggccaat ctccatcaag
     5641 tcaaaattat actacaaacc cttctgatta tattctagta caaggaaatg ccgatataaa
     5701 aaatggatat gtttttccgc gtgtctggac aacacaaatt acaaaacaag ctgacaaagg
     5761 tgatattatt cttagtgtta gggctccagt tggtgatgtt ggaaagacaa attatcatgt
     5821 tattattggg cgtggagttg ctgctataaa aggtaatgaa tttatctttc aaatattaaa
     5881 atacttgaaa gaaattggtt attggaaaag aattagtaca ggttcaactt ttgatagcat
     5941 tagctctagt gatatcaaat atgctaaaat tcaaattccc tccctccctg aacaagaagc
     6001 cattggcgaa cttttccaaa tggttgatca attgattcag ttgcaggatc aaaagctagc
     6061 aacccttaaa gaacaaaagc aaaccttcct caggaaaatg ttcccagctc aaggacaaaa
     6121 agtccctgaa atccgtttac aaggttttaa aggagagtgg gaagagaaga aattgaggga
     6181 agtatctact catagaagtg gaacggcaat tgaaaaatat tttgattcgg aaggtgaatt
     6241 taaagtcatt tcaataggta gttatggaac taataacctc tatgtagacc aaaatattag
     6301 agcagtctca aatgaactta ctaattctaa attagttgcc tccggtgaat taacaatggt
     6361 actcaatgat aagacagcga atggggcaat aatcggaaga tgtctactta ttacagaaaa
     6421 taataaatac gttgttaatc aaagaacaga aattattaga ccagatatta atatttcctc
     6481 ttactatctt ttccattatt taaatggcga atttcgtaat ggtatcatta aaattgctca
     6541 aggtggcact caaatctatg taaattattc atctgttgaa caattaaaga taaatatacc
     6601 tacattaaaa gaacaagaag ccattggtaa tttcttccaa actttagacc aacaaattgc
     6661 tcaatctgaa gaaaaactaa cagaactcaa agcactcaaa caaacattac tcaatcgttt
     6721 attcgtataa gaaaggactc acatggcaga gaaaacaact tcactccgtc aagcactatg
     6781 gcattcggcc gaccaattac gtggccaaat ggatgccaat gactataaaa actatcttct
     6841 tggacttatt ttttacaaac acttatctga taaattactc ttggctgttt gtgataatct
     6901 agaaaaacac ttcaatacct ttacagaggc tcaaaaaatc tttgaagatg cttatcaaga
     6961 tgaaggccta aaagatgacc tcatttctgt tgttactggt gatttaggtt actttatcga
     7021 accgacactg acctttgaaa aactcatcca agatgtttat cacaatactt tccaattaga
     7081 atcattagct caaggttttc gcgatattga acaaagtggt gaagactttg aaaacttatt
     7141 tgaagatatt gacctctact ctaaaaaatt aggctctact cctcaaaaac aaaatcaaac
     7201 catttctaat gtcatgaaaa cattaaatga aattgacttt gaggcagtag atggggatac
     7261 tttaggtgat gcttatgagt acttgattgg tgaatttgcc agtgaatcag gaaaaaaagc
     7321 aggtgagttc tataccccac aagctgtctc tcacttaatg actcagattg tctttttagg
     7381 tcgtgaagat caaaaaggaa tgaccctcta tgaccctgct atgggatctg gatcactcct
     7441 tttaaatgct aagaaatata gtaaccaatc cgataccgtt tcttactatg gtcaagaaat
     7501 caatacttct acttataact tggctcgtat gaacatgatg cttcatggcg ttgccatcga
     7561 gaaccaacac ttaagtaacg ctgatacctt ggatgcagac tggccaacag atgaaccaat
     7621 caactttgat ggggtgttga tgaacccacc ttattctttg aaatggtcag cgactgctgg
     7681 tttcttaacg gatccacggt tttcaagtta tggtgtatta gcaccaaaat caaaagccga
     7741 ttttgccttt ttacttcatg gtttctatca tttaaaaaat acaggaacta tggcgattgt
     7801 cttaccacac ggtgtccttt tccgtggagc tgcagaaggc aagattcgcc aaaaactact
     7861 cgaacaaggg gctatcgata ccattatcgg actcccatca aatatctttt ataatacaag
     7921 catccctacg actattatca tattaaagaa gaacagaacc aacaaagatg tcttctttat
     7981 cgatgcttca aaagagtttg ataagggtaa aaatcaaaac acaatgacag ataaccatat
     8041 caaaaagatc cttgatgctt ataagagtcg tgataattct gataagtttt catatttagc
     8101 ctcatttgat gaaatcattg aaaatgacta taacttaaat atcccacgct atgttgatac
     8161 ctttgaggaa gtcccagtta aaccactgcc agaattagct aaacagctca gtgacattga
     8221 tcaagaaatt gccaaaacaa atgccaaact agatcaactc atgaaacaac tagtcggaac
     8281 aaccaaagaa gcacaagacg aattggatac ttttagaaac taaaagacaa aaaaacacca
     8341 cagtagatga ttactactgt ggcgttttat ttaaaattga gtagtgaacc tttattgagt
     8401 acttatcaaa caagattctc cttcttcacc tataaatcaa aatagaatca tctcatctaa
     8461 ctttgtccgt ttctttgttt tatttaaaat agttatttga agttacaatg atttctcaaa
     8521 cgtttagaaa tcttaaacac ggttaagaga aattttcagg acaacttttg atccttccac
     8581 aaaaaccatg gtgcgaacca taaagtagtt ccaatcgttg atatataagc atatattaac
     8641 gttctgggag ttgtgaagct ttacaagttt ttttagacct ggtttaaaca acttttgttt
     8701 gactaaattt aaaaagcttt tcatatcagg catttcccta ctttttataa aagcgttatc
     8761 tcaatcaatt tcaatcaaaa agaactaaaa aaacaatgag tttaaatctg ggtatttaac
     8821 aggaaatccc tagcaacgac acaagtaaaa cttatgtcgt ttgattatct gcaactcaga
     8881 aatccataaa ataccctaat tgttcggctt tgtagatagc ctcctgctta ttatttacca
     8941 aaagctttga gtagatgtgg gtcaaatgat taaatactgt tctcctacta atttgtaacg
     9001 tttcctctat ttcttttata gagaatcctt tcttactcaa atttaagata gctatttcct
     9061 ggtcagttaa agattcaaca taatcctgag attcgatttg ttcaaaatac ttttttccag
     9121 aatccacttt ttttaagata gaaatgagtt gtttagggtc tatattttta tctacaaaac
     9181 cgtatgctcc tacttttttt gccctctctc tatagattga tttaacatat cctgtcaaca
     9241 taacaacttt caaatgagga gtagactgta tgagttcttt tgctatctcc aatccatttt
     9301 ctttagaaat atttgctaaa ttaatatcaa gcaatataat atcgtactta gacaaatcaa
     9361 tcgtcacatc gttaaaatga gaagtaatcg tatctatgac atctacttca tcatactgtt
     9421 ggaataacag ttggatactc tttgcaaata gtctgtggtc atcaattaat aaaatcttca
     9481 taacacaatt tccaatctat gggaagtaat attctgatag taaattgttt agtatccatt
     9541 tgtagttcta gatctccatc aaaagctgct agtgtttctt gaaatgattt caatcctctg
     9601 ccataattta tagagtgtac tttttccact acttgattgc tctcctcaat gataataata
     9661 tcagattgaa tcgttaaaga taaacgaatg tcttttccat ctccatattt tatagcattg
     9721 tttattagct cttttataaa cctataaaca atatctccat atggggatgg cactaccata
     9781 ttatctgaac agttaaaata caataaggta ttactttttc tacttttaac aatatcatca
     9841 aaaagagact gtatgttttc tttcattgtt tgattagcgg gaactattgg gtggtaagta
     9901 tccatttcat ctctgatacc agaaactaaa tcattcaact cattaacaat aaaaccatgt
     9961 gttatattac tattttctag agaaaacaag ttttttatcg ctatgatatt ttgtaaaaca
    10021 tcatcatgca aataatttga gaactccact ctcatgtttt cctctgattt cataaaatag
    10081 ttcagtcttc tattttcata gtcaaaattt tgtttggagt tttctaaata ataaaagatg
    10141 caagaaatta ccactagaca aaacaacgtg cttgcgtcaa caactataaa aaatagcaaa
    10201 aaatctttaa ctaatacctt catgagtaca atccaacaaa ggataagaag tagaaataat
    10261 agttctatac gagctttgta atcacctaac catctttggg taacaaaact caatttatca
    10321 tgtatggtct taaaatagta tattacactc attataaggg gaaacagata ccatcccaaa
    10381 ttattaaagg cttgattaac atctagcatt tctccataaa gttcactacc aagtattacc
    10441 agcaaaagta aagaaaatag tatcaatgaa cgtctaacaa ctgatatttg ttgcctaaag
    10501 gacctataat atagcactat aactagtaac ggatacaggt gtaaaatagt aaaaaccatc
    10561 aggaataaaa aattaaataa aattctatta acaaaaaggc tagttataga aacaaaaata
    10621 attattaaca agcccttatc taacttatct ttaaattgtt tgtgcccata taatattaaa
    10681 ttagtaatct caacggtata acaatagagc aatataatct gtagtccact gcttaaaaag
    10741 agaggagttc ctcttgtcag cacagatact aagtattgcc aaattgttaa ggagatgata
    10801 gaaataatcc ttttaaaaat atcaccttgt ttttggcgta atattgttgc cagccaaata
    10861 aaagctgtat caataagggt cattacaaga gctacttttt cagagaaaag aagagacagg
    10921 ttctctcgat tatagaccaa aattaacaag gctattaatt gttgcaacaa gattattgca
    10981 gtcccaaaga acttactctt t
//
LOCUS       DAXWIQ010000050         9715 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000050 DAXWIQ010000000
VERSION     DAXWIQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9715
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.050"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..1217
                     /locus_tag="ACGEUI_001482"
     CDS             <1..1217
                     /locus_tag="ACGEUI_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFF8166305.1"
                     /translation="TVYFIKKIGFLEKTAMLTVGFGSLDNKLTVDDESRDAPAGIAHF
                     LEHKLFEDESGGDISLKFTQLGAETNAFTTFNQTSFFFSTASKFQENLELLQYFVLSA
                     NITDESVSREKKIIGQEIDMYQDDADYRAYSGILQNLFPKTSLANDIAGSKASIQKIT
                     KILLETHHTYFYQPTNMSLFIVGDIDIDETFLAIQRFQTTLSYPDRKRVTVDPLHYYP
                     VIKSSSVDMDVTTAKLVVGFRGYLTLTQHSLLTYRIALKLFLSMLIGWTSKIYHTLYE
                     DGKIDDSFDVDVEIHHNFQFVLISLDTPEPIAMSNYIRQKLATIKISKEFTNEHLNLL
                     KKEMYGDFIQSLDSIEHLTHQFSLYLSDSDKETYFDIPKIIERLTLKDVVTIGKAFFE
                     KADASDFTVFPK"
     gene            1267..2292
                     /locus_tag="ACGEUI_001483"
     CDS             1267..2292
                     /locus_tag="ACGEUI_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8166306.1"
                     /translation="MSEKSLGEVLRESRVGKNITLDDIELKTGISSHYLLAMELDQFK
                     IIPEEKFDQFLKEYADIVELDFNTLKRRYRYQVNSKKDSDIKSVTEIVEEKLSKKRLQ
                     EKLLSHQSMGYGQPLEVSKNKETLVIPVKTVGLDSKSPLKTISPVSQFKSDNRLIPKK
                     NVSRMRRNSTDSRSKTSLFPIVILGTIAFAIISVIILGVWQQFEKSQRAKEAEVALLK
                     ASEQSTNGLKNNSNDRKTQVTTEGSGNYLIATVTKSKETVDISVSLTEAQSSWISLTN
                     SEIGEGGITLTQDSPTYTATLPAEITESLLTLGVTKGVSVTIDGQPVDLSPLTSTDLS
                     YITFKIQ"
     gene            2306..2848
                     /gene="pgsA"
                     /locus_tag="ACGEUI_001484"
     CDS             2306..2848
                     /gene="pgsA"
                     /locus_tag="ACGEUI_001484"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFF8166307.1"
                     /translation="MIKKENIPNLLTLVRIAMIPFFLFITSSSNKVGWHIFAAVIFAI
                     ASFTDYLDGYLARKWHVASNFGKFADPLADKMLVMSAFIMLVGLGLVPAWVSAVIICR
                     ELAVTGLRLLLVETGGKVLAAAMPGKIKTATQMLSIILLLCHWIFLGNVLLYIALFFT
                     IYSGYDYFKGASFLFKDTFK"
     gene            2850..3689
                     /locus_tag="ACGEUI_001485"
     CDS             2850..3689
                     /locus_tag="ACGEUI_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8166308.1"
                     /translation="MSAIIELKKVTFNYHKDQEKPTLDGVSFHVKQGEWLSIIGHNGS
                     GKSTTIRLIDGLLEPESGSIIVDGDLLTITNVWEIRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIAHEDIKERVNHALELVGMQNFKEKEPARLSGGQKQRVAIAGAVAMKPK
                     IIILDEATSMLDPKGRLELIKTIKNIRDDYQLTVISITHDLDEVALSDRVLVMKDGQV
                     ESTSTPEQLFARGDELLQLGLDIPFTTSVVQMLQEEGYPVDYGYLTEKELENQLCQLI
                     SKM"
     gene            3665..4507
                     /locus_tag="ACGEUI_001486"
     CDS             3665..4507
                     /locus_tag="ACGEUI_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HFF8166309.1"
                     /translation="MSINLQNVSYTYQAGTPFEGRALFNINLDILDGSYTAFIGHTGS
                     GKSTIMQLLNGLHVPTTGIVSVDKQDITNHSKNKEIKSIRKHVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGVSPEEAEALAREKLALVGISENLFEKNPFELSGGQMRRVAIAGIL
                     AMQPKVLVLDEPTAGLDPKGRKELMTIFKKLHQSGMTIVLVTHLMDDVANYADFVYVL
                     DKGKIILSGKPKTIFQQVSLLEKKQLGVPKVTKLAQRLVDRGIPISSLPITLEELREV
                     LKHG"
     gene            4500..5300
                     /locus_tag="ACGEUI_001487"
     CDS             4500..5300
                     /locus_tag="ACGEUI_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFF8166310.1"
                     /translation="MDKLILGRYIPGDSLIHRLDPRSKLLAMIIYIVIIFWANNVVTN
                     LLMLTFTLAVVFLSKIKLSFFLNGVKPMIGIILFTTLFQMFFSQGGKVIFSWWFISIT
                     DLGLSQAILIFMRFVLIIFFSTLLTLTTTPLSLSDAVESLLKPLTRFKVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIQKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGEGRTKYRQLDWQLKDSLAIGIVSLLGLLLFFLKTSL"
     gene            5672..6286
                     /locus_tag="ACGEUI_001488"
     CDS             5672..6286
                     /locus_tag="ACGEUI_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFF8166311.1"
                     /translation="MFKKENLKQRYFNFGLVALALTILAIIFAFSSKNADTKSYAKKS
                     ESKMVTIDKAPKNNHAITKEESKEKAKSIASEPIPTVENSVAPTVTEEVPVVQQEVTQ
                     TVQQVSSVAYNPNNVVLSNGNTAGIVGSQAAAQMAAATGVPQSTWEHIIARESNGNPN
                     AANASGASGLFQTMPGWGSTATVEDQVNAALKAYSAQGLSAWGY"
     gene            complement(6699..7571)
                     /gene="sdaAA"
                     /locus_tag="ACGEUI_001489"
     CDS             complement(6699..7571)
                     /gene="sdaAA"
                     /locus_tag="ACGEUI_001489"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFF8166312.1"
                     /translation="MFYTIEELVKQADQQFNGNIAELMIATEVEMSGRNREDIIKIMS
                     RNLQVMKAAVTEGLTSTKSISGLTGGDAVKMDNYIKKGNSLSDTTILNAVRNAIAVNE
                     LNAKMGLVCATPTAGSAGCLPAVLATAIEKLDLSEKEQLEFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSAAAMSAAALVKAAGGTSHQASQAIAFVIKNLLGLVCDPVAGLVEV
                     PCVKRNALGASFALVAADMALADIDSQIPVDEVIDAMYQVGSAMPTAFRETAEGGLAA
                     TPTGRRYSVEIFGE"
     gene            complement(7583..8254)
                     /gene="sdaAB"
                     /locus_tag="ACGEUI_001490"
     CDS             complement(7583..8254)
                     /gene="sdaAB"
                     /locus_tag="ACGEUI_001490"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFF8166313.1"
                     /translation="MNTQKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGDIPD
                     EVTFHLYNSFAKTYRGHGTDKALVAGIMGMGTDNPDIKNSLEIAHQKGIKIYWDILKD
                     SNAPHPNTVKISVKKADKTLSVTGVSIGGGNIQVTELNGFSVSLSMNTPTIVTVHKDI
                     PGMIAKVTDILSSNNINIATMNVTRESAGEKATMIIEVDSRECQEAANQIAKIPHIYN
                     VNFFD"
     gene            8552..9673
                     /gene="mnmA"
                     /locus_tag="ACGEUI_001491"
     CDS             8552..9673
                     /gene="mnmA"
                     /locus_tag="ACGEUI_001491"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFF8166314.1"
                     /translation="MTDNSKIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADKIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVKRDENGTVHMLRGADNGKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKSEVREIAERAGLATAKKKDSTGICFIGEKNFKQFLSQYLPAQ
                     KGRMMTIDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNQPWFVVGKDLSQNILYVGQ
                     GFYHEALMSNSLDASVIHFTREMPEEFTFECTAKFRYRQPDSHVAVHVRGDKAEVVFA
                     EPQRAITPGQAVVFYDGKECLGGGMIDMAYKNGQPCQYI"
BASE COUNT         3123 a         1619 c         1846 g         3127 t
ORIGIN      
        1 taactgttta ttttataaaa aaaatagggt ttttagaaaa aacagctatg ttgacagtag
       61 gtttcggttc tcttgacaat aagttgacag tcgatgacga atcacgagat gctccagcag
      121 gtattgcaca ctttttagaa cataaactct ttgaagatga atcaggtgga gatatttcat
      181 taaagtttac acagctagga gcagagacaa atgcctttac aactttcaac caaacgagtt
      241 tcttcttttc aacagctagt aaatttcagg aaaatttgga actccttcaa tattttgttc
      301 tttctgctaa tataacagat gaatctgtta gtagagaaaa aaaaataatc gggcaagaaa
      361 ttgatatgta tcaagatgat gctgactacc gtgcatatag tggaatcctt cagaacttat
      421 ttccaaaaac aagtttagct aatgatattg ctggctcaaa agcatccatt caaaagatca
      481 cgaaaatatt attggaaacg catcatactt atttttacca acctactaat atgagtctat
      541 tcattgtcgg tgatattgat attgacgaaa cttttttagc tattcaaagg tttcaaacca
      601 cattatccta tccagataga aaaagggtaa ctgttgatcc tttacactac tatcctgtca
      661 ttaaatcatc ttctgttgac atggatgtta ctactgccaa gctggttgtt ggttttcgag
      721 ggtatctgac cttaactcag cattcactat taacgtatcg tattgccttg aagctatttt
      781 tatcaatgct tataggttgg acttctaaaa tctatcatac gctatatgaa gatggtaaaa
      841 tagacgattc atttgatgtg gatgttgaaa ttcatcataa ttttcaattt gtattaatat
      901 cacttgatac accagagcct atcgcaatgt ctaactatat ccgtcaaaaa ttagctacta
      961 taaagatatc aaaagagttt acaaatgagc acttaaatct attaaaaaaa gaaatgtatg
     1021 gtgactttat tcagagttta gactctatag aacatttaac ccatcagttc agtctatacc
     1081 tgtctgattc tgataaagaa acatattttg atattccaaa aattatagaa agactcactc
     1141 taaaagatgt tgttacaatt ggaaaagcat tttttgaaaa agctgatgct tcagacttca
     1201 cagtcttccc aaaatagatg aaaatctgtt ataatagggt tactatatta tttttgaggt
     1261 acctgtatga gtgaaaaaag tcttggtgaa gtgttaagag aatcgcgtgt tggaaaaaat
     1321 atcacactag atgatataga attgaagaca gggatttctt cccattactt gctggcaatg
     1381 gaattggatc agtttaaaat tattccggaa gaaaaatttg atcaattttt aaaagaatat
     1441 gctgacattg ttgagttaga tttcaatact ttaaaaagac gataccgtta tcaagttaat
     1501 tccaaaaaag acagtgatat aaaatctgta actgagattg ttgaagagaa gttaagtaag
     1561 aaacgattac aagagaagtt gttatcacat cagagcatgg ggtatggtca accgttagaa
     1621 gtttctaaaa acaaagaaac cttagttatc cctgtaaaga ctgttggttt agatagcaaa
     1681 tcgccactaa aaacgatatc gcctgttagc caatttaaat cagataacag gttaattcct
     1741 aaaaaaaatg tgtcacgtat gaggcgaaat agcactgaca gtcgttctaa aacgtcacta
     1801 tttcccatag ttatattagg aacgattgct tttgcaatta tcagtgttat cattttaggt
     1861 gtttggcaac aattcgaaaa gagtcaaaga gctaaagaag ctgaagttgc tttgcttaaa
     1921 gcctctgagc agtctactaa cggtttaaaa aataattcaa atgatcgcaa aactcaagtc
     1981 acaactgagg ggtcgggtaa ttatttaata gcgactgtta ctaaatctaa agagaccgtt
     2041 gatatcagtg tgtctttaac cgaggcacaa agtagttgga tttctctaac aaattcagaa
     2101 attggtgaag gtggtataac attgactcaa gactcgccaa cttatacagc gactttacct
     2161 gcagaaataa cagaatcatt gttgacactt ggtgtgacaa aaggtgtatc tgtaacaatt
     2221 gatggacaac ctgttgatct atcaccttta acaagtactg acttaagtta tatcacgttt
     2281 aagatccaat aattaaggta gactaatgat aaaaaaagaa aatattccta atctattaac
     2341 tcttgttcga atcgcaatga ttcctttctt tctttttatt acttcctcat ctaataaggt
     2401 ggggtggcat atttttgcag ctgtgatttt tgcaattgcc agttttaccg attatctaga
     2461 tgggtatctt gcgcgtaagt ggcatgtagc cagtaatttt ggaaagtttg ccgatcctct
     2521 agcagataag atgcttgtca tgagtgcctt tatcatgtta gttggacttg gcttagttcc
     2581 tgcgtgggtg tcagctgtta ttatttgccg agaattggca gtaactggtc ttcgattact
     2641 acttgttgaa accggaggaa aggtccttgc agctgctatg ccgggaaaaa tcaaaacagc
     2701 gacacaaatg ttgtctatta ttttattact ttgccattgg atattcctag gaaacgtctt
     2761 actctatatt gctctttttt tcactattta ttctggatat gattatttta aaggagcaag
     2821 ctttcttttt aaggatacgt ttaaataaca tgtcagctat tattgaactt aaaaaagtta
     2881 catttaatta ccataaagac caagaaaaac caacattaga tggcgtatcg tttcatgtga
     2941 aacaaggtga gtggctgtct atcattggcc ataatggatc tggtaaatca acgactattc
     3001 ggttaattga tggattattg gaaccagaat cagggtctat tattgttgat ggagatctac
     3061 taaccataac taatgtttgg gaaattcgtc ataaaattgg tatggtcttt caaaaccctg
     3121 ataatcagtt tgttggagca actgttgaag atgatgttgc ttttgggctt gaaaataaag
     3181 gtattgcaca tgaagatata aaagagaggg ttaatcatgc tttagagtta gtcggcatgc
     3241 agaactttaa agaaaaagaa ccagcccgtt tatctggtgg ccaaaaacag cgcgtagcta
     3301 ttgcaggtgc agttgctatg aagcctaaaa ttattatttt agatgaagct actagtatgc
     3361 ttgaccctaa aggacgatta gagttaatca aaactataaa aaacatccgt gacgactacc
     3421 agctgactgt tatttccatt actcatgact tagatgaagt tgctcttagt gatagagttt
     3481 tagtgatgaa agatggtcaa gtggaatcaa catcaacacc agaacaatta tttgcaagag
     3541 gggatgaact actccaatta ggtcttgata tcccttttac aacatctgtt gtacagatgc
     3601 ttcaagaaga aggttatcct gttgactatg gatatctcac agaaaaggaa ttagaaaatc
     3661 agttatgtca attaatctcc aaaatgtaag ttatacttat caagcaggga ctccttttga
     3721 agggcgtgcc ctttttaaca ttaatttaga tattttagat ggttcttata cggcttttat
     3781 cggacatact ggatccggga aatcgaccat tatgcaattg ctaaatggtt tacacgttcc
     3841 gacaacggga attgtcagcg tagataaaca agatataacg aatcactcta aaaataaaga
     3901 gattaaatct attcgaaaac atgtaggatt agtttttcaa tttccagaaa gtcaactatt
     3961 tgaagagaca gttttgaaag atgttgcttt tggccctcaa aattttgggg tctctccgga
     4021 ggaagcagaa gctttagcgc gtgaaaaact agctcttgtt gggatttcag aaaatctttt
     4081 tgaaaagaat ccatttgaac tctcaggggg tcaaatgcgt cgtgttgcta ttgcgggtat
     4141 ccttgctatg cagccaaagg ttttagtttt agatgaaccg actgctggtc ttgatcctaa
     4201 agggcgtaaa gaattaatga ctatttttaa aaagttacac caatctggca tgactattgt
     4261 tttagtaacc catctgatgg atgatgtggc caattacgca gattttgtat atgttcttga
     4321 caaaggtaaa attattttat caggaaaacc caaaacaatt tttcaacaag tcagtttgct
     4381 tgagaaaaaa cagttaggag ttcctaaggt gactaaactt gctcaaaggc ttgttgatag
     4441 aggaatacct atctcttctt tgcctattac cttagaggag ctaagagagg tactaaaaca
     4501 tggataagct gattcttggt cgttatattc ccggagattc tctcattcat cgtcttgacc
     4561 caagaagtaa attattagcg atgattattt atattgtcat tatcttttgg gctaacaatg
     4621 ttgtgacaaa tttgttgatg ttgactttta ctttagcagt tgtttttttg tcaaaaatca
     4681 agttgtcttt ctttttaaat ggggttaaac caatgattgg tattatcctc ttcacgactt
     4741 tatttcagat gtttttctca caaggaggaa aagtaatttt tagttggtgg ttcattagca
     4801 taactgattt aggattgagc caagctattt taatttttat gcgtttcgtt ctaattattt
     4861 tcttttcaac gctgttaacg ttgacgacaa cacctttaag cctatctgat gcagttgaat
     4921 cattattaaa acctttaaca agatttaaag ttccagctca cgagatcgga ctaatgcttt
     4981 ctttaagttt acgttttgtg cctactttaa tggatgatac cacgcgtatt atgaatgcac
     5041 aaagggcccg tggagttgat tttggagagg gaaatttaat tcaaaaagtt aaatccatta
     5101 ttcctatttt aatccctctt ttcgcttcaa gctttaaaag agccgacgca ttagctattg
     5161 ctatggaagc tagaggttat cagggaggag aagggcgaac taaatatcgt caattagact
     5221 ggcagctaaa agatagttta gctattggaa tagtttcttt attgggtttg ctactttttt
     5281 tcttgaaaac ctctttgtaa tattttttag cacaaaactt ttctttatta gtaagaagtg
     5341 aattgtacct caaaagttag atacaatatc taatgattga ggtacttttc ttcatttgag
     5401 gaattgaaaa ccgctatctc aactatatta atcctcccaa taataacaaa tcgaacgaaa
     5461 tctaaaagga ctgagtcctg ttcaatacag aactaaattc ttagtttaat gatattatcc
     5521 cgctttttaa tgttgctaag aagaagagtt ttatgatttt ttacaacttc ttataatatc
     5581 aatgtagttg taatattttt aatatatctt caataacgag gtaataatta aaagatatta
     5641 tggataacgg agaaaagtga ggtattattg aatgtttaag aaagaaaatt taaaacaacg
     5701 ttattttaat tttggattag tagcgttagc tctaacaata ttagccatca tttttgcctt
     5761 ctcaagtaaa aatgctgata ctaagtctta tgctaagaag tcagaaagta aaatggtaac
     5821 aatcgacaag gctccaaaaa ataatcatgc tattactaaa gaagaaagca aagaaaaagc
     5881 aaagagcatt gcttcggagc ctattcccac agtagaaaac tctgtagctc cgacagtaac
     5941 agaggaagta ccggttgttc agcaagaagt gactcaaact gttcagcagg tatcttcagt
     6001 agcctataat ccaaacaatg tggtactttc caatggaaat actgctggta ttgtaggaag
     6061 tcaagcggcg gcacagatgg cagcagcaac aggtgttcca caatcaactt gggaacatat
     6121 aattgcgcgt gaatctaatg gaaatcctaa cgcagctaat gcttctgggg catcagggtt
     6181 gttccagaca atgccaggtt ggggttctac agcaacggtt gaagatcaag tcaatgcagc
     6241 cttgaaagcc tatagtgcac aaggtttatc agcttggggt tactaaataa acatggctgg
     6301 aagaaataaa acctattgtt agtatttact aacaataggt ttttggtatt taattgtctt
     6361 tatgatttgt agtgatgaat tagtctcaaa acaaaacatt gccttatcgg gaatatagct
     6421 cgtattattt aggaaaactg accagcactt aagtgcctct gatatttaag gcagttatac
     6481 tgataacaag caggaaaaaa actcttaaat gtttataagg tcattagcaa ttttcagtat
     6541 tttacactac tacacttttg agttttaaca atttactaga agttgtttct aaggggaaac
     6601 aacatagctt gatagctatc tattagtcat tttagtgaat atatagcaga aaaataaaac
     6661 gctttcaaat atttctaaga atttttctaa ctatctattt attcgccaaa tatttctacg
     6721 ctgtaacgtc gtccagttgg agtagcggct aaaccacctt ctgctgtttc acggaaagca
     6781 gttggcatgg cagagccgac ttgatacata gcatcaataa cttcgtcaac aggaatttga
     6841 gaatcaatat cagctaaagc catatcagct gcgacaaggg cgaaacttgc tcctagggca
     6901 ttgcgtttga cacaaggcac ttctactagg ccggcaacag gatcacagac aagtcctaat
     6961 aaatttttga taacaaacgc aatagcttga ctagcttgat gagatgttcc accggctgct
     7021 ttaacaagag cagctgcact catagcagct gcagaaccaa cttctgcttg gcatccacct
     7081 tctgcaccag aaatagaggc gttatttcca ataacaagac caaaagctcc tgcagtaaat
     7141 aaaaactcta attgttcttt ttctgataag tcaagttttt caatagctgt tgcgagcact
     7201 gctggtaagc atcctgcact tcctgcagtg ggtgttgcac agacaagtcc cattttagca
     7261 tttaattcgt ttacagctat agcatttcta acagcattaa gaatagtagt atcagaaagg
     7321 ctgttccctt ttttaatgta gttatccatt ttgaccgcat cgccccctgt caaaccactg
     7381 atagatttgg ttgaggttag tccttctgtt acagctgctt tcataacttg taaattgcgt
     7441 gacatgattt tgataatatc ttctcgattt cttccagaca tctccacttc cgtggcaatc
     7501 atgagttcag caatatttcc attaaattgt tggtcagctt gtttgacaag ttcttcaata
     7561 gtataaaaca tagctttcct ttctagtcaa agaagttgac attataaata tgaggaattt
     7621 ttgcaatttg gttggctgct tcttggcact ctctagaatc gacttctata atcatagtag
     7681 ctttttcacc agcggattca cgtgtaacat tcattgtggc gatattaata ttattggaag
     7741 ataggatatc agttaccttt gcgatcattc cgggaatatc tttgtgtaca gtaactatag
     7801 taggagtatt catggataac gagacagaaa aaccatttag ttctgttact tgaatgtttc
     7861 ctcctccaat tgaaacacca gtaacactaa gtgttttgtc agcttttttg acactgattt
     7921 taacagtatt gggatgggga gcattactat cttttaagat atcccaatag atttttatcc
     7981 ctttttgatg agcaatttct agagaatttt taatatcagg attatcagta cccataccca
     8041 taattcctgc aacaagagcc ttatcagtac catgtcctct gtaggtttta gcaaatgaat
     8101 tataaaggtg aaacgtcact tcatcaggaa tatctccaaa gatggaatgg actaccttac
     8161 caattctgac ggcaccagct gtgtgactac ttgatggacc aatcattact ggcccaatga
     8221 tgtcaaaaac tgattgaaat ttttgtgtat tcataggcaa ctccgttttt tatttctatg
     8281 tttattataa caaaaaaata tcaaaaaaca gcaatataat tataaaaaat tatttttata
     8341 ataaaaaatt attgagcaat aaaatgattg gcattccacg cagtaattgt tataataagg
     8401 tttgcgctga tggcgcttca aaatcgacct tcaaatcgac ttttgaaaac gacgaccttc
     8461 aatcgtacgt taactgataa gaaaaagaat cataatattc ataatatgat gattatcttt
     8521 ttctatctta ttattgatgg gagaattttt tatgacagat aactcaaaga tacgtgttgt
     8581 cgttggtatg agtggtggcg ttgattcatc tgtaacagct ctacttttaa aggagcaagg
     8641 ttatgatgtt attggtgtct tcatgaaaaa ctgggacgat acagatgaat ttggtgtttg
     8701 cacagctact gaagattaca aagatgtagc agccgttgct gataaaatcg gtattcctta
     8761 ctattcagtc aattttgaaa aagaatattg ggacagagta tttgaatatt tcttggccga
     8821 ataccgagca ggtcgtactc caaatcctga tgtcatgtgt aataaggaaa taaaatttaa
     8881 agctttcctt gactatgcca tgactttagg tgcagattat gtagcaacgg gacactatgc
     8941 tcaagtcaag cgtgatgaaa atggtacggt tcacatgtta cgtggagctg ataatggcaa
     9001 agaccaaacc tactttttaa gtcaactatc acaggaacaa ctacaaaaga ctttattccc
     9061 ccttggtcat ttgcaaaagt cagaagttag agaaatcgct gagcgagcag ggcttgcaac
     9121 ggctaaaaag aaagattcaa caggtatttg ttttattggt gaaaagaatt tcaaacagtt
     9181 tttgagccag tacttaccgg cacaaaaagg tcgcatgatg actattgatg gtcgcgatat
     9241 gggagaacat gccggcctta tgtattatac tattggtcag cgtgggggtc tgggcattgg
     9301 tggtcagcat ggtggggata atcaaccttg gtttgttgtc ggtaaagatt tgtcgcaaaa
     9361 tattttatac gttggtcaag gtttctacca tgaagcccta atgtcaaata gtcttgacgc
     9421 ctcagtcatc cattttacac gtgagatgcc agaagagttc acatttgaat gtactgctaa
     9481 atttcgctat cgtcaacccg attcacacgt ggcggtgcat gttcgaggag ataaggcaga
     9541 agttgttttt gcagagccac agcgcgcgat tactcctggt caagctgttg ttttctatga
     9601 cggaaaagaa tgccttggtg gtggtatgat tgacatggct tataaaaatg ggcagccttg
     9661 tcagtacatt taatgaatct tttattaaga taaaaagaaa gggacattca acaat
//
LOCUS       DAXWIQ010000051         9591 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000051 DAXWIQ010000000
VERSION     DAXWIQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9591
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.051"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(155..1222)
                     /locus_tag="ACGEUI_001492"
     CDS             complement(155..1222)
                     /locus_tag="ACGEUI_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HFF8166315.1"
                     /translation="MRRAENNKHSRYSIRKLSVGVTSIAIASLFLGKVAYAVDGIPPI
                     SLTQKTTATTSENWHHIDKDGLIPLGISLEAAKEEFKKEVEESRLSEAQKETYKQKIK
                     TAPDKDKLLFTYHSEYMTAVKDLPASTESTTQPVEAPVQETQASASDSMVTGDSTSVT
                     TDSPEETPSSESPVAPALSEAPAQPAESEEPSVAASSEETPSPSTPAAPSTPAAPETP
                     EEPAAPSQPAESEESSVAATTSPSPSTPAESETQTPPAVTKDSDKPSSAAEKPAASSL
                     VSEQTVQQPTSKRSSDKKEEQEQSYSPNRSLSRQVRAHESGKYLPSTGEKAQPLFIAT
                     MTLMSLFGSLLVTKRQKETKK"
     gene            complement(1319..4813)
                     /gene="scpA/B"
                     /locus_tag="ACGEUI_001493"
     CDS             complement(1319..4813)
                     /gene="scpA/B"
                     /locus_tag="ACGEUI_001493"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HFF8166316.1"
                     /translation="MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQA
                     VETPQPTAVSEEAPSSKETKTPQTPDDAEETIADDANDLAPQAPAKTADTPATSKATI
                     RDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKEDLEKAK
                     KEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRLEGA
                     MPEAQLLLMRVEIVNGLADYARNYAQAIIDAVNLGAKVINMSFGNAALAYANLPDETK
                     KAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSPD
                     KQLTETATVKTADQQDKEMPVLSTNRFEPNKAYDYAYANRGMKEDDFKDVKGKIALIE
                     RGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRKDGLLLKENP
                     QKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAKL
                     SGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYFS
                     PRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELYY
                     QATVQTDKVDGKLFALAPKALYETSWQKITIPANSSKQVTIPIDVSQFSKDLLAPMKN
                     GYFLEGFVRFKQDPTKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDA
                     KDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGTF
                     AKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGNV
                     VWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPISS
                     GAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPVYRERIAYTYMDED
                     LPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLEG
                     HSNKPEQDGSDQAPDKKPETKPEQDGSGQAPDKKPETKPEQDGSGQTPDKKPETKPEK
                     DSSGQTPGKTPQKGQPSRTLEKRSSKRALATKASTKDQLPTTNDKDTNRLHLLKLVMT
                     TFFLGLVAHIFKTKRTED"
     gene            5247..6633
                     /locus_tag="ACGEUI_001494"
                     /pseudo
     CDS             5247..6633
                     /locus_tag="ACGEUI_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611311.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(7031..7876)
                     /locus_tag="ACGEUI_001495"
     CDS             complement(7031..7876)
                     /locus_tag="ACGEUI_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis inhibitor protein"
                     /protein_id="HFF8166317.1"
                     /translation="MNIRNKIENSKTLLFTSLVAVALLGATQPVSAETYTSRNFDWSG
                     DDWSGDDWPEDDWSGDGLSKYDRSGVGLSQYGWSKYGWSSDKEEWPEDWPEDDWSSDK
                     KDETEDKTRPPYGEALGTGYEKRDDWGGPGTVATDPYTPPYGGALGTGYEKRDDWRGP
                     GHIPKPENEQSPNPSHIPEPPQIEWPQWNGFDGLSSGPSDWGQSEDTPRFPSEPRVTE
                     KPQHTPQKNPQESDFDRGFSAGLKAKNSGRGIDFEGFQYGGWSDEYKKGYMQAFGTPY
                     TPSAT"
     gene            complement(8065..9393)
                     /gene="emm"
                     /locus_tag="ACGEUI_001496"
     CDS             complement(8065..9393)
                     /gene="emm"
                     /locus_tag="ACGEUI_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein"
                     /protein_id="HFF8166318.1"
                     /translation="MAKNNTNRHYSLRKLKTGTASVAVALTVLGAGFANQTEVKANGD
                     GNPREVIEDLAANNPAIQNIRLRHENKDLKARLENAMEVAGRDFKRAEELEKAKQALE
                     DQRKDLETKLKELQQDYDLAKESTSWDRQRLEKELEEKKEALELAIDQASRDYHRATA
                     LEKELEEKKKALELAIDQASQDYNRANVLEKELETITREQEINRNLLGNAKLELDQLS
                     SEKEQLTIEKAKLEEEKQISDASRQSLRRDLDASREAKKQVEKDLANLTAELDKVKEE
                     KQISDASRQGLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEESKKLTEKEKA
                     ELQAKLEAEAKALKEQLAKQAEELAKLRAGKASDSQTPDTKPGNKAVPGKGQAPQAGT
                     KPNQNKAPMKETKRQLPSTGETANPFFTAAALTVMATAGVAAVVKRKEEN"
BASE COUNT         2396 a         1970 c         1945 g         3280 t
ORIGIN      
        1 catcttttat cacagcaact gaagagataa gcaaaagaca attgttgaca gctatttgtc
       61 ctaacttctt tttgatgagg cagcacatct aaaaaaatag agagaaccaa attggttctc
      121 tctattaaat aagctcttat cagtttaagt aatactattt tttagtttct ttttggcgtt
      181 ttgtgactaa aagactgcca aatagagaca tcaaagtcat agtagctata aagagtggct
      241 gtgctttttc acctgttgaa ggcaagtact tacctgactc atgggcccta acctgtcttg
      301 acaatgagcg atttggagag taagactgtt cttgctcttc ttttttatca gaagatctct
      361 ttgaagttgg ttgttgaacg gtttgttctg aaacaagtga agaggctgct ggtttttcag
      421 ctgctgaaga tggcttatca gagtctttag taacagctgg tggcgtctga gtctctgatt
      481 cagctggagt tgatggagac gggcttgtcg tagctgctac tgaagattct tcactctcag
      541 ctggttgaga tggagctgct ggttcttcag gagtttctgg agccgctgga gttgatgggg
      601 ctgctggagt tgatggagat ggggtttcct cagaagatgc tgctactgaa ggttcctcac
      661 tctcagctgg ttgagctgga gcctcagata aagctggggc cactggactt tccgaagatg
      721 gggtttcctc aggagaatca gtcgtaactg atgttgaatc acctgtcacc atcgaatctg
      781 aagctgatgc ctgtgtctcc tgcacgggtg cctcaactgg ctgagtagta gactcagtag
      841 acgctggaag atccttaacg gctgtcatat actcactatg atacgtgaat aatagcttat
      901 ctttgtctgg tgcagtttta attttttgtt tatacgtttc tttttgtgct tcagataaac
      961 gtgattcttc tacttctttt ttaaattcct ctttggcagc ttctaagctt atacctaaag
     1021 gaataaggcc atccttatca atatgatgcc aattttctga tgtagtggct gtagtctttt
     1081 gagtaagaga gattggaggg atgccatcta cggcataggc aacctttcct aaaaagagac
     1141 tcgcaattgc tatactcgtt accccaacgc tcagtttgcg aatggaatag cggctgtgtt
     1201 tgttattttc tgctctacgc atattgtcct cacatatcta tttttatact gctatgcctt
     1261 cttttgaaaa gaagactgat ctaactcatc atcaatagta gccatctgat cagttgtact
     1321 aatcttcagt gcgttttgtt ttaaagatat gagctactaa tcccaagaag aaagtggtca
     1381 taactaactt aaggagatgt aaacgatttg tatccttgtc attagtcgtt ggtaactgat
     1441 cttttgttga tgcttttgta gctaaagcac gcttagaaga tcgtttctct agagtacgag
     1501 aaggttgacc tttttgagga gttttacctg gtgtttgacc tgaactatct ttttctggtt
     1561 tagtttctgg ttttttatct ggtgtttgac ctgaaccgtc ttgttctggt ttagtttctg
     1621 gttttttatc tggtgcttga cctgaaccgt cttgttctgg tttagtttct ggttttttgt
     1681 ctggtgcttg atctgaaccg tcttgttctg gtttattaga gtggccttcc aatagcttag
     1741 tcactggtgt ataagtgatg ttaccagcca tatcttcaac aacataagta aagtctgaca
     1801 ttttcaatgg aacagtagcg ccttccattg tttcagcctc ttcaggaaga gtaaaggtac
     1861 catcttcatt tggagaaata tactctgttg ttggcagatc ctcatccata taagtgtaag
     1921 caatacgctc acggtaaacc ggttggctgg tttttggttt agatgcaagt gtcaaacgac
     1981 gatcttctgt tgagaatgtt gccgatgttg cgacttcagg tgtcgtattg tctacaatca
     2041 catcaaaatc agtgtgttgt tcttttgcac ctgagctaat cggagtgtag cgaacacgat
     2101 aggtgtatgt tccgttagca acaactttgc cgtctttatc tttaccgtcc caacgcgttt
     2161 tttcaaaacg ggttgaacca agtgtgcttg ccaagtcatt gttgtagttt ttaacaactt
     2221 gctcggttac ctcacttgtc caaacaacat ttccttcttt gtccaagact tcagccacaa
     2281 ggtttttagc attacgcaag aaagtacctt ggaattggac ataatctctg ttaccgtccc
     2341 catttggaga gatcgcagca tatggcttgc cattagcgtg acggtggata tagtagtggc
     2401 tatcatcgtc ttgttttgca aaagtacctg caaaaatggt ttctgtgatc tctgaagatt
     2461 cgatatcctc tatgttttca accccttctt tgacagcttt aataatcgtc catggattag
     2521 actctgtagt aagtgctgta aagttatttt tcagagcgta aaactgtaat ccatcaccat
     2581 ctaattggtc tttggcatca ctatttgctt catgatagta gctgctaccg tctttgctat
     2641 catagattgg tttttctaag gctgacagat tgccaaaatc acctcggaaa ccaatatagg
     2701 gaatactcat aagctcttct tttgtaggat cttgtttgaa acgaacaaaa ccttctaaga
     2761 aatagccatt tttcattggg gcaagcaagt ccttgctaaa ttgactaaca tcgattggaa
     2821 tggtgacttg tttgctgcta ttggctggaa ttgtgatttt ttgccatgat gtctcataca
     2881 atgctttagg agccaaggca aagagttttc catctacttt atctgtttga acagttgctt
     2941 ggtaatacaa ctcttgaggt ttatcagatt tgttgtgaac tgttactgtt acttcaaatt
     3001 tatcagaaac attgttcagg tgaaccttgc ttgaggtatt atccttatct gtcacataca
     3061 tcgttgctgc tgaagctttt ttagcatcga ctgctcctgc tccttgttgg cgaggagaaa
     3121 aataagcttt ttcatcttca tcatataagg cagttgctga gctcatcaat acttttttag
     3181 ctaaatcaag acgctctgat ggtgtcatat caggatactg tgtctcatat tgcttttgca
     3241 acagtcccat gatacccgct actaatggcg cagacatact agttccagaa agtttggcat
     3301 acttgttgtt agccactgat gacaaaatat cttggccggg tgctgcaata tctggcttaa
     3361 tattgccgtc agctgtcaga ccccagcttg agaagcggct tagtttggtg ccacttgctg
     3421 ttggcaatac cttaggtgtc gcattgaagg tgatggtttt ttggggattc tcttttaata
     3481 agagaccatc ttttcgactg ataaaggccg caggcatctg atcaacattt ggcaattcaa
     3541 tcgggaagcc cttgtcctga ttgtcataga tcaagactcc tacagcacca gcttttttag
     3601 cgtttgcaat cttatctttg aaatcaatat cgccacgttc aataagggca atcttacctt
     3661 tgacatcctt aaaatcatcc tctttcatcc cacgattagc ataagcatag tcgtaagcct
     3721 tgtttggctc aaaacggttt gttgaaagaa caggcatttc tttatcttgc tgatcggctg
     3781 ttttgaccgt agcagtttca gtgagctgtt tatctgggct gtaagaagca actgtcaatg
     3841 ttgagtctgc cgctgcaggt gtcccaacca ccccataatc aggatgatct gctagaggta
     3901 gacgggtctt gcccccaaag ctactatcat taccagctga ggtcacaatg ctaacacctt
     3961 ttgatttggc atagtcaaag gcttttttgg tttcgtctgg aaggttggca taggctagtg
     4021 cagcattacc aaagctcata ttaatcacct tagctcccaa gttgacagca tctatgatag
     4081 cttgagcgta gttacgagca tagtctgcta gtccatttac aatttcgaca cgcatcaaaa
     4141 gcaattgagc ctcaggcatc gcaccttcta ggcggtaagg ttctttcgtt tcagatggag
     4201 catttcctga caagatccct gacacgtgtg tgccgtgctc ttgatcgaca gcggttttac
     4261 catctttact atagtcgtgg taataagcaa ccttatcatt gacccactcg ccataggtaa
     4321 taccgtgctc ttttttagct ttttcaagat cttcttttga ttggtaacgt gctttggttt
     4381 tgtctgttaa gcgccacgct tcatgatttt tatcaaaacc agcatcaatc actgcaacaa
     4441 cagtcccagc tcctttgcct gctttttcct gcagggtttt gacctgagaa gggtcgttca
     4501 aatccctaat agtcgctttt gaggttgctg gtgtatcagc agttttagca ggagcttgag
     4561 gggctagatc attagcgtca tctgctattg tttcttctgc gtcatcagga gtttgtgggg
     4621 ttttggtttc ctttgatgat ggtgcttcct cagaaaccgc tgttggttgt ggggtttcta
     4681 cagcttgttc ggtagcagga gtgtcttctg tcacagtatt tgctttaatg tctgattgtg
     4741 cattgagcaa gatgctcgta gacatgagcg caatggcaag tttatcaaat ggtaattttt
     4801 gttttttacg caatgtgtcg tcctttcctg atgagagact ttgtctttat gaccattaag
     4861 aaaagagaga atcaaacgat tctctcctag ctgtccatct gtgcgtagag aaataaggat
     4921 aacgtctttt ttaaagagca aacaggggtt atttgcatat gatacagcta tttttcttgc
     4981 ttttgtcata atgattaaat gtatctcatt tatcaattat attaataaca tatttattat
     5041 aaaacaaaga cgattattag taaaggtcag ctttttaaga gttgcttagt ttgtgacctc
     5101 cttatcctca ttcttttaga cataaaagag agaaccgatc ggttctctct tcagactgaa
     5161 gacaaagtcc ttagaatcgc ttttctaggg gctttttatg atatttagga gtataatata
     5221 agaaaaagga cttgtgaggt tctcctatgt tacacaaaga aaaacctgat tataaccgca
     5281 atcagtacgg tttctatacc cttgaccagt tagtgcctga tgaacatttc ctgcgccaag
     5341 tggaagctgt gattgacttc gacttcatct atgacctagt agaagacacc tattcgtctg
     5401 ataatggtcg ccctagcctt gatcctgtca tgttggttaa aatccctctg attcagtgct
     5461 tttatggcat tcgttccatg cgtcagacca tcaaggatat tgaagtgaat actgcctatc
     5521 gctggttttt aggtttgact ttggatgaca aggtgccaca tttcacaacc tatggcaaaa
     5581 attattcccg tcgctttcaa gaaaaaggct tgattgagtc catttttacg catattgttg
     5641 ggctatgtat caatacaggc ctgattgacc cgacggagat tttcatagat gggactcata
     5701 ttaaggctgc ggctaataat cgcaagttca tcaaccgaga ggtggaaaaa caagccaagt
     5761 tcatgagtga acagctagaa atcgaaatca atcgggatag ggagaagcat ggaaaaaagc
     5821 cgctagggcc cgccaaagag ctagagcccg tcgctaagaa aatctccaca accgatccag
     5881 aaagtggctg gtttcacaaa ggtgatcata aggaagtctt cgcttattct gctcaggtgg
     5941 cttgtgacaa gtatggatgg gcgcttgctt atagtgttga ggctggcaat atccatgata
     6001 gtcaggcttt tcctgcactt tttgccaagc ttgaaccctt gaaaccacag tttattattg
     6061 cggattccgg ctataacgtt cccagcattg ccaagtttct gattgataag gagatcactc
     6121 ctgtccttcc ttacacacga ccaagaggaa agaaagacct tctacgtccg aaagaattga
     6181 tttatgatga gcactttgat tgtgtcatct gccctgaaca tcaagccttg acctaccgca
     6241 ctacgaccag agaaggctac cgagaataca aaagtgaccc tgtaatttgt gccaattgtc
     6301 ccctgttatc tgtatgtacg accagtaaga actaccaaaa agttgtcaca cggcatattt
     6361 ggaaagacta tttggcacag ccaaggaata ccacaatctc tgctatacga gagagaaagg
     6421 caagtccaaa atggaagata cggttggact gactttggca tgcttaaatc tcaaaaaact
     6481 ggtcaaaagg atggcgggag agccttttta ttttgttcag atgaggtggt ttaagcacgg
     6541 aaacagccat ttttatctaa aaacactaaa aaagacaaac accaaatcga tgtttgtctt
     6601 caatctgttc tagagacttt ttctttattc tgagcgatag ttgtcataca ttgctttttt
     6661 agatgagtac actgcactct tttataatgg ttttagtgtc cgtggaataa agagttcagc
     6721 attatactca ctattgttaa cttcactatc actttattca atgtatcata gtctaaccaa
     6781 gcttgctagt ttccccaatg atagtcacca gcaattcagg tagtggcaat atgctacccg
     6841 tctcctaagc aagaatcttt ctcggactca gatagtccat agcgcagcgc atcaatcact
     6901 cctatcagaa attttagcaa gacataaata acattttaca gccctacatc acctatggaa
     6961 tcagaatttt tcgtaaaaat ccctaagaag cgtggcaaaa gcagcactaa actattccta
     7021 tcgcatccct ttacgttgct gatggtgtat atggtgtacc gaaggcttgc atgtaacctt
     7081 ttttatattc gtctgaccag ccaccatact ggaaaccttc aaaatcaata cctctacctg
     7141 agttttttgc tttcaagcca gctgaaaacc ctctatcaaa atctgattct tgtggatttt
     7201 tttgaggagt atgttgcggt ttttctgtca cacgaggttc acttgggaaa cgaggggtgt
     7261 cctcagattg gccccaatca gaggggccag atgataatcc atcaaagcca ttccactgag
     7321 gccactcaat ctgaggaggt tcaggaatat gagatgggtt tggtgattgt tcgttctcag
     7381 gtttaggaat atgtccaggt cctctccaat catcacgttt ttcataccct gtacctaatg
     7441 ctcctccata tggtggagtg taagggtcag ttgccaccgt accaggtcct ccccaatcat
     7501 cacgtttttc ataccctgta cctaatgctt ctccatatgg tggtctcgtt ttatcttctg
     7561 tctcatcttt tttatcgcta gaccagtcat cttcaggcca atcttcaggc cattcttctt
     7621 tatcgctaga ccagccatat ttagaccagc catattgaga caaaccaact ccagaccggt
     7681 catatttaga caaaccatct ccagaccagt catcttcagg ccagtcatct ccagaccagt
     7741 catctccaga ccagtcaaaa ttgcgtgatg tatacgtttc ggctgaaact ggttgtgtag
     7801 ctcctagtag agccacggct acaagggatg taaatagtag tgttttacta ttttcaatct
     7861 tatttctaat attcattagt attctctcct taataaatta tttcgttaat gttttcatta
     7921 taaaacaaag acgattatta gtaaaggtca gctttttaag agttgtttag tttgtgacct
     7981 ctccttaacc tcattcttct atacataaaa gagagaaccg actggttctc tcttgatgtt
     8041 cactcagtat tacaaagtga tagcttagtt ttcttctttg cgttttacaa ctgctgctac
     8101 tccagctgtt gccataacag taagggctgc cgctgtgaag aatgggttag ctgtttcacc
     8161 tgttgatggt aactgtctct tagtttcctt cattggtgct ttgttttggt taggttttgt
     8221 acctgcttgt ggtgcttgac ctttacctgg aacagctttg tttcctggtt ttgtatcagg
     8281 ggtttgtgag tctgatgctt ttccagctct tagttttgcg agttcttcag cttgtttcgc
     8341 taattgttct ttgagtgctt ttgcttctgc ttcaagtttt gcttgtagtt cagctttttc
     8401 tttttctgtt aatttcttgc tttcttcaag ctctttgtta agtttttcaa gagcagctaa
     8461 tttgctgttt gcttcttcta aagctttttc aacttgtttc ttagcttcac gtgatgcgtc
     8521 caagtcacgg cgaaggcctt gacggcttgc gtctgagatt tgtttttctt ctttaacctt
     8581 atcaagttca gcagtcaagt ttgctaaatc tttttcaacc tgtttcttag cttcacgtga
     8641 tgcgtccaag tcacgacgaa ggctttgacg acttgcgtct gagatttgtt tttcttcctc
     8701 aagttttgct ttttcgatcg ttagctgctc tttttcagat gaaagttgat caagttcaag
     8761 ttttgcattg cctaaaagat tacgattaat ctcttgttct ctagtaatcg tttctaactc
     8821 tttttctaag acgttagctc tattatagtc ctgactcgct tggtctatcg ctaattcaag
     8881 agctttcttt ttctcttcta actctttttc taaagcggta gctctatggt agtcccgact
     8941 tgcctggtct atcgctaatt caagagcttc ctttttctct tctaactctt tttcaagtct
     9001 ttgtctatcc caacttgttg attcctttgc taagtcatag tcttgttgta gttcttttaa
     9061 tttagtttct aaatctttac gctggtcttc taaggcttgt tttgcttttt caagttcttc
     9121 agctctctta aaatctcttc ctgcaacttc cattgcattc tctaatctcg cttttaagtc
     9181 cttgttttcg tgacgtaaac gtatattttg tattgcggga ttgtttgctg caagatcttc
     9241 tataacttcc ctaggattac catcaccgtt agccttaacc tctgtttgat tcgcaaaacc
     9301 tgcccctaaa acagtcaaag ctaccgctac tgaagccgtt cctgttttta attttctaag
     9361 cgaatagtgt ctattcgtgt tatttttagc catttttatg ctccttatgc tatcattttt
     9421 taggaagaaa ccctcatttt cagggtttaa ctctaataat tctattgtaa ataataaaag
     9481 ccaaaaggta aagaccagct tttttagctt ttgccacctt tttgacctaa atgctattaa
     9541 tgatgaatct gtttttgagt tgctacagtt aggctgtcta agttgtggag g
//
LOCUS       DAXWIQ010000052         9590 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000052 DAXWIQ010000000
VERSION     DAXWIQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9590
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.052"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            202..852
                     /locus_tag="ACGEUI_001497"
     CDS             202..852
                     /locus_tag="ACGEUI_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFF8166319.1"
                     /translation="MKERLEHYSTSSIYSGFEVYLPLVLQTITDVIIAENIKSKKETG
                     FKLYEHFTSRIKSEASMIEKCQRKQLPLTSKSALKIIKDSIGIRIICGFIDDIYRMVD
                     LLKSIPGMSVNTEKDYILNAKPNGYRSYHLILELETHFPDILGEKKGCYFIEVQLRTI
                     AQDSWASLEHQMKYKHQVANAEMITRELKRCADELASCDVTMQTIRQLIQETTEEE"
     gene            855..1523
                     /locus_tag="ACGEUI_001498"
     CDS             855..1523
                     /locus_tag="ACGEUI_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8166320.1"
                     /translation="MKILLAEDEWQMSNVLTTAMTHQGYDVDVVFNGQEAIDKAKDNA
                     YDIMILDIMMPIKSGIEALKEIRASGNCSHIIMLTAMAEINDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMERRVESFTPQVLQFAGVTLNINEQELSAGNAIRLASKEGKLMAFL
                     MLNQGKYLDTKTLYQHVWSDQEDYDTSIVWVYISYLRQKLLAIQANVIIAGDKDTSYC
                     LLEK"
     gene            1532..2764
                     /locus_tag="ACGEUI_001499"
     CDS             1532..2764
                     /locus_tag="ACGEUI_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8166321.1"
                     /translation="MFNRIRIRFIMIASIAIFIILSSIVGIINTARCYQSQQEINRIL
                     HLISSNKGKLPGTTESSKRLGTKLSEDSLSQFRYYSVIFNANGHLLSSNTANISALDR
                     EEAQYFARLFAKSGEEKGSYRHQDSVYSYLITQLPNEEKLVVILDTTFYFRSVGDLLA
                     VSVMLAFGGFIFFVVLVSLFSGMVIKPFVQNYEKQRRFITNAGHELKTPLAIISANNE
                     LVELMTGESEWTKSTSDQVKRLTGLINQMITLARLEEQPDVVLHMVDFSAIAQDAAED
                     FKSLVLKDGKRFDLTIQPNIMIKAEEKSLFELVTILVDNANKYCDPKGLVKVSLTTIG
                     RRRKRAKLEVSNTYLEGKSIDYSRFFERFYREDESHNSKEKGYGIGLSMAESMVKLFK
                     GTITVNYKNDAIVFTVVI"
     gene            3058..3936
                     /gene="mvk"
                     /locus_tag="ACGEUI_001500"
     CDS             3058..3936
                     /gene="mvk"
                     /locus_tag="ACGEUI_001500"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFF8166322.1"
                     /translation="MNENIGYGKAHSKIILIGEHAVVYGYPAIALPLTDIEVVCHIFP
                     ADKPLVFDFYDTLSTAIYASLDYLQRLQEPIAYEIVSQVPQKRGMGSSAAVSIAAIRA
                     VFSYCQEPLSDDLLEILVNKAEIIAHTNPSGLDAKTCLSDHAIKFIRNIGFETIEIAL
                     NGYLIIADTGIHGHTREAVNKVAQFEETNLPYLAKLGALTQALERAINQKNKVAIGQL
                     MTQAHSALKAIGVSISKADQLVEAALRAGALGAKMTGGGLGGCMIALADTKDMAEKIS
                     HRLKEEGAVNTWIQML"
     gene            3918..4862
                     /gene="mvaD"
                     /locus_tag="ACGEUI_001501"
     CDS             3918..4862
                     /gene="mvaD"
                     /locus_tag="ACGEUI_001501"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFF8166323.1"
                     /translation="MDPNVITVTSYANIAIIKYWGKENQAKMIPSTSSISLTLENMFT
                     TTSVSFLPDTATSDQFYINGILQNDEEHTKISAIIDQFRQPGQAFVKMETQNNMPTAA
                     GLSSSSSGLSALVKACDQLFDTQLDQKALAQKAKFASGSSSRSFFGPVAAWDKDSGAI
                     YKVETDLKMAMIMLVLNAAKKPISSREGMKLCRDTSTTFDQWVEQSAIDYQHMLTYLK
                     TNNFEKVGQLTEANALAMHATTKTANPPFSYLTKESYQAMEAVKELRQEGFACYFTMD
                     AGPNVKVLCLEKDLAQLAERLGKNYRIIVSKTKDLPDV"
     gene            4855..5862
                     /locus_tag="ACGEUI_001502"
     CDS             4855..5862
                     /locus_tag="ACGEUI_001502"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFF8166324.1"
                     /translation="MSNYCVQTGGKLYLTGEYAILTPGQKALIHFIPLMMTAEISPAA
                     HIQLASDMFSHKAGMTPDASYALIQATVKTFADYLGQSIDQLEPFSLIITGKMERDGK
                     KFGIGSSGSVTLLTLKALSAYYQITLTPELLFKLAAYTLLKQGDNGSMGDIACIAYQT
                     LVAYTSFDREQVSNWLQTMPLKKLLVKDWGYHIQVIQPALPCDFLVGWTKIPAISRQM
                     IQQVTASITPAFLRTSYQLTQSAMVALQEGHKEELKKSLAGASHLLKEIHPAIYHPKL
                     VTLVAACQKQDAVAKSSGSGGGDCGIALAFNQDARDTLISKWQEADIALLYQERWGEN
                     D"
     gene            5855..6844
                     /gene="fni"
                     /locus_tag="ACGEUI_001503"
     CDS             5855..6844
                     /gene="fni"
                     /locus_tag="ACGEUI_001503"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFF8166325.1"
                     /translation="MTNRKDDHIKYALKYQSPYNAFDDIELIHHSLPSYDLSDIDLST
                     HFAGQDFDFPFYINAMTGGSQKGKAVNEKLAKVAAATGIVMVTGSYSAALKNPNDDSY
                     RLHEVADNLKLATNIGLDKPVALGQQTVQEMQPLFLQVHVNVMQELLMPEGERVFHTW
                     KKHLAEYASQIPVPVILKEVGFGMDVNSIKLAHDLGIQTFDISGRGGTSFAYIENQRG
                     GDRSYLNDWGQTTVQCLLNAQGLMDQVEILASGGVRHPLDMIKCFVLGARAVGLSRTV
                     LELVEKYPTERVIAIVNGWKEELKIIMCALDCKTIKELKGVDYLLYGRLQQVN"
     gene            complement(7074..8351)
                     /locus_tag="ACGEUI_001504"
     CDS             complement(7074..8351)
                     /locus_tag="ACGEUI_001504"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFF8166326.1"
                     /translation="MTKTNLNWSGFSKKTFEERLQLIEKFKLLNAENLNQLKTDVLLP
                     IQTANQMTENVLGRLALPFSIAPDFLVNGSTYQMPFVTEEPSVVAAASFAAKLIKRSG
                     GFKAQTLNRQMIGQIVLYDIDQIDNAKAAILHKTKKLIALANKAYPSIVKRGGGARTI
                     HLEEKGEFLIFYLTVDTQEAMGANMVNTMMEALVPDLTRLSKGHCLMAILSNYATESL
                     VTTSCEIPVRLLDHDKTKSLQLAQKIELASRLAQVDPYRATTHNKGIFNGIDAVVIAT
                     GNDWRAIEAGAHAYASRNGSYQGLSQWHFDQDKQVLLGQMTLPMPIASKGGSIGLNPT
                     VSIAHDLLNQPDAKTLAQLIASVGLAQNFAALKALTSSGIQAGHMKLHAKSLALLAGA
                     TQDEIAPLVNALLADKPINLEKAHFYLSQLRQS"
     gene            complement(8338..9513)
                     /locus_tag="ACGEUI_001505"
     CDS             complement(8338..9513)
                     /locus_tag="ACGEUI_001505"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFF8166327.1"
                     /translation="MTIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSV
                     APITEDIITLAASAADQILTDEDRAKIDMVILATESSTDQSKASAIYVHHLVGIQPFA
                     RSFEVKQACYSATAALDYAKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIAL
                     LVTANPRILALNEDNVAQTRDIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQK
                     RENLQLSDLAAVCFHIPFPKLALKGLNNIMDNTVPPEHREKLIEAFQASISYSKQIGN
                     IYTGSLYLGLLSLLENSKVLQSGDKIGFFSYGSGAVSEFYSGQLVAGYDKMLMTNRQA
                     LLDQRTRLSVSKYEDLFYEQVQLDDNGNANFDIYLTGKFALTAIKEHRRIYHTNDKN"
BASE COUNT         3024 a         1721 c         1940 g         2905 t
ORIGIN      
        1 aacgtaatcg ttatagatca agtttactat gattttgttt gtaattcaat ggtattttta
       61 aaaaataacg tacaaaaaat atatgaaagc gatttaaaaa caaacgatac agatcatttg
      121 aagttatata gttagaaata aagataaccg atcattgcag agatttaagc atttgttgag
      181 aaaatttgtt ataataagcg tatgaaagaa agactagaac attattctac cagctctata
      241 tatagcggat ttgaagtgta cttgccgttg gtactacaga caataacaga tgtgattatt
      301 gctgagaata tcaaaagcaa aaaagaaacg gggtttaaac tatacgaaca ctttacatcc
      361 cgtattaaat cggaagcttc gatgattgaa aaatgtcaac gcaaacagct tcctctaaca
      421 agcaaatctg cgttaaaaat cattaaagat agtatcggta ttcgcattat ttgtggtttt
      481 attgatgata tttatcggat ggtagacttg ctcaaaagca ttcctggtat gtcagttaac
      541 actgaaaaag attatatttt aaatgccaag cctaatggct atagatcata tcatctcatt
      601 ttggaattag aaacacattt tccagatatt ttaggggaaa aaaaaggatg ttattttata
      661 gaagtgcagt tgcgaaccat tgctcaagac tcttgggcta gcttggaaca tcaaatgaag
      721 tacaaacatc aagttgctaa tgcagaaatg attacaagag agttaaagcg ttgtgcagac
      781 gagttggcat cttgtgatgt taccatgcaa accattcgtc aattgattca agaaacgacg
      841 gaggaagagt aaccatgaaa attttgttag ctgaagacga atggcagatg tctaatgttt
      901 tgacaactgc catgactcat caaggttatg acgttgatgt ggtttttaac ggtcaagagg
      961 caattgataa agccaaagac aatgcatatg atattatgat tttagatatc atgatgccga
     1021 ttaagtctgg cattgaggct ttaaaggaga ttcgcgcttc aggaaattgc tcacatatta
     1081 ttatgctgac ggctatggca gaaattaatg accgtgttac cggactagat gcgggagctg
     1141 atgattatct gactaaacct ttctcattaa aagaactttt agctagatta aggtcgatgg
     1201 aaagacgagt agaaagtttt actcctcagg tattacaatt tgctggtgtc acattaaata
     1261 ttaatgagca agaattgagt gccggtaatg ctatacgatt agcttctaaa gaaggtaaat
     1321 taatggcctt tttaatgtta aatcaaggaa agtacttgga tactaaaacg ctttaccaac
     1381 atgtttggtc agaccaagaa gattatgata cttctattgt ttgggtttat atttcttatc
     1441 ttcgtcaaaa attattagct attcaagcaa atgtcattat tgcaggtgat aaagatacta
     1501 gctattgttt attagaaaaa taggaggtga catgtttaat cgtatccgca ttcgttttat
     1561 catgattgct tctattgcca tttttattat tttaagttca attgttggta ttattaacac
     1621 ggcacgctgt taccaaagtc aacaagaaat taaccgtatt ttacatttaa tttcctcaaa
     1681 taaaggtaaa ttacctggca ctacggaaag ctctaaacga cttggaacga aactgtctga
     1741 agatagccta agccagtttc ggtattatag tgtcattttt aatgctaatg gacatctact
     1801 ttcttctaat actgctaata tttcagcttt agatagagaa gaagcccaat attttgctag
     1861 actttttgct aagtctgggg aagaaaaagg cagttaccgt caccaagata gtgtttattc
     1921 gtacttgatt actcagctgc caaatgaaga aaaactggtt gtaattttag atactacctt
     1981 ttattttcgt agtgtaggag atttattagc tgtttcagtg atgttggcct ttggaggatt
     2041 tatctttttt gttgttttag tgagtctttt ttccggtatg gtcatcaaac cttttgtcca
     2101 aaattatgag aagcagcgtc gctttattac taatgcaggc cacgagttaa aaacaccact
     2161 agccattatc tcagccaata atgaattggt cgaattaatg actggagaat cagaatggac
     2221 caagagtaca agtgatcagg tgaagcgatt gacaggcttg attaatcaaa tgattacttt
     2281 ggctcgtttg gaggaacagc cagatgtggt attacatatg gtagactttt cagctattgc
     2341 tcaagatgca gctgaagatt ttaagagtct ggttttgaaa gatggtaaac gttttgattt
     2401 gacgattcaa ccgaatatta tgattaaagc ggaggaaaag tcattgtttg aacttgtgac
     2461 aattttagtt gataatgcca ataaatattg tgatcctaaa ggcttggtca aggtatcttt
     2521 aaccactatt ggaagaagac gaaaacgagc gaaattagaa gtttctaata cctatttgga
     2581 aggaaaatcc attgattaca gccgcttctt tgaacgcttt tatcgcgaag acgaatccca
     2641 taatagcaaa gaaaaaggtt atggaattgg tttatctatg gcagaaagta tggttaagtt
     2701 atttaaagga acgataactg taaattataa aaacgatgct attgttttta cagtggtgat
     2761 ttgacttaaa catttttcag tacctttagc tagtaataat gtctaggtaa aggtaataaa
     2821 cgatgcttaa ccagcccatg aggctggttt ttaacgttta atttagtaga agagtgatta
     2881 aagaaggtag tttgaaataa gaaaattttt gttatgatct taaaaagagt agatagtctt
     2941 tattgttaat ttcagtgaag aaatgatttt aaaaagatat atcatcgcat tttctaatca
     3001 tttagtgtaa aatagtaagg taaaagttta gaagaattag taatgaaaag acaaaaaatg
     3061 aacgaaaaca ttggatatgg taaggcacac agtaagatca ttttgatagg tgagcatgct
     3121 gttgtgtatg gctacccagc tattgctttg cctttaacag atattgaggt ggtttgtcat
     3181 atttttccag ctgataagcc attggtcttt gatttttatg atactctatc aacagccatt
     3241 tatgcttcct tggattattt gcagcgacta caagaaccaa ttgcttatga gattgtttca
     3301 caagtgccac aaaagcgtgg tatggggtct tcggcagccg tttctattgc agctattaga
     3361 gccgtttttt cttattgtca agaacccctc tcggatgatt tgttggaaat tttagtcaac
     3421 aaggcagaaa ttattgccca caccaatcct agtggtttag atgccaagac atgccttagt
     3481 gaccatgcca ttaagtttat ccgaaacatt ggctttgaaa ctatcgaaat cgccttaaat
     3541 ggttatctta tcattgcaga tacagggatt cacggtcata cacgtgaggc cgtcaataag
     3601 gtagcacagt ttgaggaaac gaatttgccc tatctggcta aactaggagc tttgacacaa
     3661 gcccttgaaa gagcgattaa ccaaaaaaat aaggtggcta tcggtcagct catgacacaa
     3721 gcgcactctg ctttaaaggc cattggcgtt agcatcagta aagctgacca acttgtggag
     3781 gctgccctaa gagcaggtgc tttaggcgct aagatgacag gcggtggctt gggtggctgt
     3841 atgattgccc tagcagatac caaagacatg gccgaaaaaa tcagtcacag attaaaagaa
     3901 gaaggagccg taaacacgtg gatccaaatg ttataaccgt aacatcctat gctaacattg
     3961 ctattatcaa atattgggga aaagagaatc aagctaagat gattccttcg acctctagca
     4021 tttctttgac tttggaaaac atgttcacca caaccagcgt ttccttctta ccagatactg
     4081 caaccagtga tcagttttac attaacggta tcttgcaaaa tgacgaagaa cataccaaaa
     4141 tttctgctat cattgaccaa tttcgccaac ctggtcaggc ttttgtaaag atggaaactc
     4201 aaaataatat gccaacagct gcaggtttat catcaagttc cagtggctta tcagccttgg
     4261 ttaaagcctg tgaccaatta tttgacaccc agctagatca gaaagcttta gctcaaaagg
     4321 ctaagttcgc ctcaggatca tcttctcgtt ctttttttgg cccagtcgct gcttgggaca
     4381 aagatagtgg tgctatttac aaggttgaga ctgacttgaa aatggccatg attatgctgg
     4441 tcttaaatgc tgcgaaaaag ccaatttcta gccgagaggg catgaagtta tgccgcgata
     4501 cctcaactac atttgatcaa tgggtagaac aatcggcaat cgattaccaa catatgctta
     4561 cctatctcaa aactaataat tttgagaaag ttggtcagtt aacagaagct aatgccttgg
     4621 ccatgcatgc cacgacaaag acagccaatc ctcctttttc ttacctaaca aaagagtctt
     4681 accaggctat ggaggctgtg aaagaactgc gtcaagaagg ttttgcttgt tattttacca
     4741 tggacgcagg tccaaatgtg aaggtcctat gtttagaaaa agacttggct caactagcag
     4801 aacgacttgg caagaactac cgtattatcg tttcaaaaac aaaggattta ccagatgtct
     4861 aattattgtg tgcaaacagg tgggaaacta tacctcacag gcgaatatgc tatcttaaca
     4921 ccaggacaaa aagccttaat tcactttatt ccattgatga tgacagcaga aattagccca
     4981 gcagcccata ttcaattagc ttcagatatg ttttcccata aagcgggcat gacacccgat
     5041 gcctcttatg cactgattca agcaacggtt aaaacctttg ctgattatct aggacagtca
     5101 attgaccaac tggagccatt ttccctaatt ataacgggaa aaatggagcg cgatggcaaa
     5161 aaatttggca ttggttcaag tggtagcgtc accctcttaa ccttaaaggc cttatcggcc
     5221 tattatcaga ttactttaac cccagagtta ctctttaaac tagcggctta taccttgctt
     5281 aagcaaggag ataatgggtc tatgggggat attgcctgta tcgcttatca gactttagtc
     5341 gcctatacct cctttgaccg agaacaggtc agtaattggc tgcagaccat gcccttaaaa
     5401 aaacttctcg tcaaagattg gggttaccat atccaagtca ttcaaccagc cctgccttgt
     5461 gactttttgg ttggctggac taaaatacct gctatttcaa ggcagatgat tcaacaagtg
     5521 acagcgagca ttaccccagc tttcttaaga acaagttacc agctaacgca atcagctatg
     5581 gtagctttgc aagaaggtca taaggaagaa ctcaagaaaa gtttagcagg agcaagtcat
     5641 ctcctaaaag agattcatcc agctatctac catcctaagc tagtaacctt ggtagctgct
     5701 tgtcagaagc aagatgctgt tgctaaatct tcaggttctg gtggtggaga ttgtggcatc
     5761 gcgcttgcct ttaatcagga tgctagagat acccttattt ccaaatggca agaagctgat
     5821 atcgcattac tttatcaaga aaggtgggga gagaatgact aaccgtaaag atgatcacat
     5881 caaatatgct ctcaagtacc aatcgcctta taatgctttt gatgacatag aactcataca
     5941 ccattcctta cctagctatg atttgtctga tattgatctc agtactcatt ttgctgggca
     6001 agacttcgac tttccctttt acatcaatgc catgacagga ggaagtcaaa aaggcaaagc
     6061 tgtcaatgaa aaattggcca aagtagcagc agcaacaggg attgtcatgg tgacagggtc
     6121 ttatagcgct gctttaaaaa atcctaacga cgattcctat cgtttacatg aggtggcaga
     6181 taacttgaaa ctagccacga atattggtct agataaacct gtggcgctag gacaacaaac
     6241 ggttcaagaa atgcagcccc tctttttaca ggttcatgtg aatgtgatgc aagagttgct
     6301 gatgccagag ggtgagcgcg tctttcatac ctggaaaaaa cacctcgctg aatacgctag
     6361 tcaaatacca gttcctgtca ttctcaaaga agttggtttt ggcatggatg tcaatagtat
     6421 caagctagca catgacctag gcattcaaac ctttgatatt tcaggtagag gaggaacttc
     6481 atttgcttac attgaaaatc aaagaggggg agaccgctct tacttaaacg attggggaca
     6541 aaccactgtt cagtgcttac tgaatgcaca aggactgatg gaccaagtgg aaatcttagc
     6601 ttcgggtggt gtcagacacc ccttggacat gattaagtgt tttgtcttag gagcacgtgc
     6661 agtgggactc tcacgcaccg ttttagaatt ggtcgaaaaa tacccaaccg agcgtgtgat
     6721 tgctatcgtt aatggctgga aagaagaatt aaaaatcatt atgtgtgctc ttgactgtaa
     6781 aactattaaa gaattaaagg gagtcgacta cttactatat ggacgcttgc agcaggtcaa
     6841 ttaggcttca tcacctctac aatagtgtca cctactacaa agatgataga gcttttttag
     6901 aacataatat aaaatattta taaatacctc atcaaactca gaatagagta gatgagtaaa
     6961 tagagtatgt tatggagact atttctaaga cttgtatgta tagaaataaa gattaaacac
     7021 ccatgtaaac caattgttta catgggtgtt tgccttatat tagttgttta aatctaagac
     7081 tgtcttagct gggataagta aaaatgtgct ttttctagat ttattggttt atcagctagt
     7141 aaagcattaa ctaaaggagc aatttcgtct tgggttgccc ccgccaaaag agctaatgat
     7201 ttcgcatgta gtttcatgtg accagcttgg atgccagatg aggtcagagc ttttagtgca
     7261 gcaaagtttt gagctaaccc cacagatgca atcaattggg ctaatgtttt ggcatcaggt
     7321 tgattaagaa gatcatgtgc gatagaaaca gtagggttaa gcccgataga tcccccctta
     7381 ctagcaatag gcatagggag ggtcatttgg ccaagcagaa cttgtttatc ttggtcaaaa
     7441 tgccactgac taagtccttg atagctacca tttcttgagg cataagcatg ggcccctgct
     7501 tcaatagcac gccagtcatt tcctgtggct attaccactg catcaatgcc attaaaaata
     7561 cctttattat gagtagtagc ccggtaagga tctacttgag ctagtcggct ggctagctct
     7621 attttttgag ctaactgtag ggattttgtt ttatcgtgat ctaaaaggcg cacgggaatc
     7681 tcacaactag tagtaacaag cgactctgtt gcgtaattag ataaaatcgc cattagacaa
     7741 tgccccttag acagtcttgt taaatcagga acaagagctt ccatcatagt attgaccata
     7801 tttgctccca tagcttcttg ggtatcaaca gtcagataga aaatcaaaaa ttctcctttt
     7861 tcttccaaat gaatggttct agcgcctcca cctcttttaa caatggaagg ataagcttta
     7921 tttgccaatg caattagctt ttttgtttta tgaaggatgg cggctttagc gttatctatt
     7981 tggtcgatat cgtaaagaac aatttgacca atcatttgtc ggtttagggt ttgagcttta
     8041 aaaccacctg agcgtttgat tagttttgct gcgaaagatg ctgcagcaac aacagaaggt
     8101 tcttccgtga caaaaggcat ctgataagtt gaaccgttga caagaaaatc aggagctatg
     8161 ctaaagggca aagccaatcg tcctaagaca ttttcagtca tttgattagc tgtttggata
     8221 ggcaaaagaa cgtctgtttt gagttgattt aagttttcag cattaagtag tttaaatttt
     8281 tcgataagtt ggaggcgttc ttcgaatgtt ttctttgaaa aaccgctcca gttaagatta
     8341 gtttttgtca ttggtatgat agatccttcg atgctccttg atggctgtta gagcaaattt
     8401 tccagttaag taaatgtcaa aattggcatt accattatca tctaattgga cttgttcgta
     8461 gaaaaggtct tcgtatttag aaacagaaag acgtgttcgt tgatccagta aagcttgtcg
     8521 attagtcatt aacattttat cgtacccagc aactaactga ccggaataaa attcacttac
     8581 ggcaccggaa ccatagctaa aaaaaccaat tttatctcca gattgtaata ctttactatt
     8641 ttcaagtaaa gataacaatc ctagataaag tgagccagta taaatatttc caatttgttt
     8701 gctataacta atagaagctt gaaaggcttc tattagtttt tccctgtgtt caggaggtac
     8761 tgtgttatcc ataatgttat ttagaccttt tagggctaac ttgggaaatg gaatatggaa
     8821 acaaactgca gcaagatcag ataactgaag gttttctctt ttctgataag cttgccacgt
     8881 ggtttctaag caattgagat attgcttggt agagtaaata ccatcaacat aaggtgtgaa
     8941 actatagtta ggcctccaaa agtccataat atccctagtt tgagccacat tatcttcatt
     9001 tagagcaaga atacgagggt tagcagttac caaaagagca atactaccag agccttgagt
     9061 cgattcccca ggagatccta caccgtatct agcaatatca ctagcaataa caaggacacg
     9121 agaatctggc ttagaagcca catgcagttt agcatagtct agagcagcag ttgcgctata
     9181 gcaggcttgt tttacttcaa aggaacgtgc aaaaggctgg ataccaacta agtgatgtac
     9241 atagatagct gatgcctttg actgatcagt actcgattca gttgccaaaa taaccatatc
     9301 aatcttagct cgatcttcgt cggttaaaat ttgatctgct gcagaagcag ctaaagtaat
     9361 aatgtcttca gtgattggtg ccacactaaa agactcaatg agtagccctt gactaaattt
     9421 tgctggatcc acttggcgtg caagcgctaa atcttccaat ttcaagacat attgactggt
     9481 tgcaaaacca atcttatcaa ttccaattgt catatttgtc cttcttttag ttatttttat
     9541 agtcttaccc attctatcac attttactgc aaaattagac tgataaagtt
//
LOCUS       DAXWIQ010000053         9541 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000053 DAXWIQ010000000
VERSION     DAXWIQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9541
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.053"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            90..1010
                     /gene="lmb"
                     /locus_tag="ACGEUI_001506"
     CDS             90..1010
                     /gene="lmb"
                     /locus_tag="ACGEUI_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HFF8166328.1"
                     /translation="MKKGFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVDVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGRLDPKHKDSYTKNAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
     gene            1023..3500
                     /locus_tag="ACGEUI_001507"
     CDS             1023..3500
                     /locus_tag="ACGEUI_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HFF8166329.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGSATKDNQIAYIDD
                     SKGKAKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYRFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPAPGRRKAPIPDVTP
                     NPGQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEED
                     GLIFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTEDDDSGSDH
                     SKPSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHF
                     HYIGFGELEQYELDEVANWVKAKGQADELAAALDQEQGKEKPLFDTKKVSRKVTKDGK
                     VGYMMPKDGKDYFYARDQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSS
                     LPMHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRDQIATIKYVMQHPEVRPDIWSKPG
                     HEESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFATPDGYIFDPRDVLAKE
                     TFVWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPKEKQQ
                     ADKSNENQQPSEASKEEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIF
                     QPEGVQFYNKNGELVTYDIKTLQQINP"
     gene            3669..3869
                     /locus_tag="ACGEUI_001508"
     CDS             3669..3869
                     /locus_tag="ACGEUI_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFF8166330.1"
                     /translation="MSEEKLKSKIEQASGGLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDAKEAVEGAVDAVKEKLK"
     gene            complement(3950..4576)
                     /locus_tag="ACGEUI_001509"
     CDS             complement(3950..4576)
                     /locus_tag="ACGEUI_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8166331.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEIDLEVAPGQILGIFGQSGCGKTS
                     LSRVLAGFLQPKSGEVLVDGSHLPNKAFRPVQLIQQHPEQTMNPLWPMKKSLEEAYYP
                     SQDLRDAFGIQEKWLKRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIIISHEFDMLEKLCDACYMIEENRTQLFKHT"
     gene            complement(4560..5363)
                     /locus_tag="ACGEUI_001510"
     CDS             complement(4560..5363)
                     /locus_tag="ACGEUI_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8166332.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEVKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNAAVTGDMIYRGQSLTSKRIKQLRGKEMTLIPQSVN
                     YLDPSMKVKHQVRLGISENAKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDTVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGGGEQLQTEFARRLWRTLPQQDFLKGVTHDLRG"
     gene            complement(5375..6196)
                     /locus_tag="ACGEUI_001511"
     CDS             complement(5375..6196)
                     /locus_tag="ACGEUI_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8166333.1"
                     /translation="MILKRRTMVLWQLGIAISLILSILALNLYFYRTPLETNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVFLATVFGVLAGLGNSLID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYDLKN
                     KAFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(6193..7170)
                     /locus_tag="ACGEUI_001512"
     CDS             complement(6193..7170)
                     /locus_tag="ACGEUI_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8166334.1"
                     /translation="MKRTTIIIIWKIIRCVTLIFGVSVLTFVLLKQSPVDPVMASVNY
                     DTSLTPAQYKAIAHHYGLDKPALVQYFIWLKNVIQGDLGTSLVYRQPVSDIIRSRAGA
                     SFILMGLSWILSGLIGFILGTLSAFHQGKLLDRVVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRVKHLMLPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETQWQIFKHHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(7283..8911)
                     /locus_tag="ACGEUI_001513"
     CDS             complement(7283..8911)
                     /locus_tag="ACGEUI_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8166335.1"
                     /translation="MSKYLKYFSIITLFLTGLILVACQQQKPQTKERQRKQRPKDELV
                     VSMGAKLPHEFDPKDRYGVHNEGNITHSTLLKRSPELDIKGELAKTYHLSEDGLTWSF
                     DLHDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELADKKVKGTRLLDIPSNDVRGLSLPYVKK
                     GVITDSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDKTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGDLDAAFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHHPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSSDLDKANEYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGVHSHGHDWSLLTNIAEWTWDESTK"
     gene            9090..9356
                     /locus_tag="ACGEUI_001514"
     CDS             9090..9356
                     /locus_tag="ACGEUI_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166336.1"
                     /translation="MTYFRLKKELNWSLRNKGIVVYEGNQKLQIAINTGKNNSEKVFF
                     DTFLEVVRAAATFSTAKNICFGLGLLRSQKQEIGYHISRVVHVF"
BASE COUNT         3144 a         1842 c         1880 g         2675 t
ORIGIN      
        1 aaaagatggt agttgccatt gacaaagcat tgataaagga gtaaaattaa ctggttaata
       61 actggttaaa ttataattga ggaggtacta tgaaaaaagg tttttttctc atggctatgg
      121 tcgtgagttt agtaatgata gcagggtgtg ataagtcagc aaaccccaaa cagcctacgc
      181 aaggcatgtc agttgtaacc agcttttacc caatgtatgc gatgacaaaa gaagtatctg
      241 gagacctcaa tgatgtgagg atgatccaat caggtgcagg cattcattcc tttgaaccgt
      301 ctgtaaatga tgtggcagct atttatgacg cggatttgtt tgtttaccat tcacatacct
      361 tagaagcttg ggcaagggat ctagacccta atttaaaaaa atcaaaggtt gatgtgtttg
      421 aagcgtcaaa acctttgaca ctagatagag tcaaagggct agaagatatg gaagtcacac
      481 aaggcattga tcctgcgaca ctttatgacc cacatacctg gacagatccc gttttagctg
      541 gtgaggaagc tgttaatatc gctaaagagc taggacgttt ggatcctaaa cacaaagaca
      601 gttacactaa aaatgctaag gctttcaaaa aagaagcaga gcaactaact gaagaataca
      661 ctcaaaaatt taaaaaggtg cgctcaaaaa cattcgtgac gcagcacacg gcattttctt
      721 atctggctaa acgattcggc ttgaaacaac ttggtatctc gggcatttcc ccagagcaag
      781 agccctctcc tcgccaattg aaagaaattc aagactttgt taaagaatac aacgtcaaga
      841 ctatttttgc agaagacaat gtcaatccca aaattgctca tgctattgcg aaatcaacag
      901 gagctaaagt aaagacatta agtccacttg aagctgctcc aagcggaaac aagacatatc
      961 tagaaaatct tagagcaaat ttggaagtgc tctatcaaca gttgaagtaa aggagattat
     1021 tagtgaagaa aacatatggt tatatcggct cagttgctgc tattttacta gctactcata
     1081 ttggaagtta ccaacttggt aagcatcata tgggttcagc aacaaaggac aatcaaattg
     1141 cctatattga tgatagcaaa ggtaaggcaa aagcccctaa aacaaacaaa acgatggatc
     1201 aaatcagtgc tgaagaaggc atctctgctg aacagatcgt agtcaaaatt actgaccaag
     1261 gctatgtgac ctcacatggt gaccattatc atttttacaa tgggaaagtt ccttatgatg
     1321 cgattattag tgaagagttg ttgatgacgg atcctaatta ccgttttaaa caatcagacg
     1381 ttatcaatga aatcttagac ggttacgtta ttaaagtcaa tggcaactat tatgtttacc
     1441 tcaagccagg tagcaagcgc aaaaacattc gaaccaaaca acaaattgct gagcaagtag
     1501 ccaaaggaac taaagaagct aaagaaaaag gtttagctca agtggcccat ctcagtaaag
     1561 aagaagttgc ggcagtcaat gaagcaaaaa gacaaggacg ctatactaca gacgatggct
     1621 atatttttag tccgacagat atcattgatg atttaggaga tgcttattta gtacctcatg
     1681 gtaatcacta tcattatatt cctaaaaagg atttgtctcc aagtgagcta gctgctgcac
     1741 aagcctactg gagtcaaaaa caaggtcgag gtgctagacc gtctgattac cgcccgacac
     1801 cagccccagc cccaggtcgt aggaaagccc caattcctga tgtgacgcct aaccctggac
     1861 aaggtcatca gccagataac ggtggctatc atccagcgcc tcctaggcca aatgatgcgt
     1921 cacaaaacaa acaccaaaga gatgagttta aaggaaaaac ctttaaggaa cttttagatc
     1981 aactacaccg tcttgatttg aaataccgtc atgtggaaga agatgggttg atttttgaac
     2041 cgactcaagt gatcaaatca aacgcttttg ggtatgtggt gcctcatgga gatcattatc
     2101 atattatccc aagaagtcag ttatcacctc ttgaaatgga attagcagat cgatacttag
     2161 ccggccaaac tgaggacgat gactcaggtt cagatcactc aaaaccatca gataaagaag
     2221 tgacacatac ctttcttggt catcgcatca aagcttacgg aaaaggctta gatggtaaac
     2281 catatgatac gagtgatgct tatgttttta gtaaagaatc cattcattca gtggataaat
     2341 caggagttac agctaaacac ggagatcatt tccactatat aggatttgga gaacttgaac
     2401 aatatgagtt ggatgaggtc gctaactggg tgaaagcaaa aggtcaagct gatgagcttg
     2461 ctgctgcttt ggatcaggaa caaggcaaag aaaaaccact ctttgacact aaaaaagtga
     2521 gtcgcaaagt aacaaaagat ggtaaagtgg gctatatgat gccaaaagat ggcaaggact
     2581 atttctatgc tcgtgatcaa cttgatttga ctcagattgc ctttgccgaa caagaactaa
     2641 tgcttaaaga taagaaacat taccgttatg acattgttga cacaggtatt gagccacgac
     2701 ttgctgtaga tgtgtcaagt ctgccgatgc atgctggtaa tgctacttac gatactggaa
     2761 gttcgtttgt tatccctcat attgatcata tccatgtcgt tccgtattca tggttgacgc
     2821 gcgatcagat tgcaacaatc aagtatgtga tgcaacaccc cgaagttcgt ccggatatat
     2881 ggtctaagcc agggcatgaa gagtcaggtt cggtcattcc aaatgttacg cctcttgata
     2941 aacgtgctgg tatgccaaac tggcaaatta tccattctgc tgaagaagtt caaaaagccc
     3001 tagcagaagg tcgttttgca acaccagacg gctatatttt cgatccacga gatgttttgg
     3061 ccaaagaaac ttttgtatgg aaagatggct cctttagcat cccaagagca gatggcagtt
     3121 cattgagaac cattaataaa tctgatctat cccaagctga gtggcaacaa gctcaagagt
     3181 tattggcaaa gaaaaacgct ggtgatgcta ctgatacgga taaacccaaa gaaaagcaac
     3241 aggcagataa gagcaatgaa aaccaacagc caagtgaagc cagtaaagaa gaagaaaaag
     3301 aatcagatga ctttatagac agtttaccag actatggtct agatagagca accctagaag
     3361 atcatatcaa tcaattagca caaaaagcta atatcgatcc taagtatctc attttccaac
     3421 cagaaggtgt ccaattttat aataaaaatg gtgaattggt aacttatgat atcaagacac
     3481 ttcaacaaat aaacccttaa ccaaaagaag atctcattgt taaagcactg ctttgtcaaa
     3541 gcaagttacg gtgattttga agtcatttta tgtaacgagt agtgataaaa gttggataat
     3601 agcggttttc ttttgcaaag aaatggtatc catgttagaa tgataaaaaa aagaggagga
     3661 ttctaatcat gtcagaagaa aaattaaaat caaaaattga gcaagcatct ggtggcctaa
     3721 aagaaggtgc tggtaagttg acaggtgata aagagctaga agcaaaagga tttgttgaaa
     3781 aaacaattgc taaaggcaaa gaacttgcag acgatgcaaa ggaagcggtt gaaggtgctg
     3841 tagatgccgt taaggaaaaa ttaaaataat taaaaaacca gctgtaagct ggttttttta
     3901 atggtgtgat tttgttagga aaagactcaa taggtaataa ccgtaggtgt taggtatgct
     3961 tgaacagttg agtacgattc tcttcaatca tatagcacgc gtcacaaagt ttttctaaca
     4021 tatcaaactc atgactaata atgatgagac ctaaatttct atcctttaca atctccaaca
     4081 ggcttttcca tacactagct tgtgtaatgc tatccaacat agtagtcatt tcatctgcaa
     4141 taaggtattt cgtctctgga tgtaatgaac gcacaatcga aaagcgttgc aattcccctc
     4201 ccgagagttc actaggacga cgctttagcc atttttcttg aatgccaaaa gcatctcgca
     4261 aatcttgact tggatagtag gcttcttcca aacttttttt cataggccat aaaggattca
     4321 tggtttgttc aggatgttgt tggataagtt gtacaggtct aaatgctttg ttaggcaaat
     4381 gactgccatc aactaatact tcaccagatt taggctgcaa aaaaccagca agcactctgg
     4441 ataaactcgt ttttccacac ccactttgtc caaatatccc taagatttga ccaggtgcta
     4501 cctctaaatc aatctcctta aaaagccatt gatctttttt atgataaaag ccaagctttt
     4561 tagcctctaa ggtcatgagt aactcctttc aaaaagtctt gctgagggag tgtgcgccat
     4621 aaacgtctcg caaattctgt ttgtaattgc tccccacctc cgctaaaaaa gctagctggg
     4681 gctgtttcaa tagcttttcc ctctttaaaa atagtaatac gatccgcaat ttgactagct
     4741 gctacaatat catgggtgat aaatatgacg cttattcctt tatctgcaaa ggagcgtagt
     4801 tgatctaaaa ccatttgcag agcatctgga tgtaatccag gggtgggctc atccgcaata
     4861 atcaaagaaa ccgtatcact aatacacgtt gtaaacaaaa cacgtcggag cattccgcca
     4921 gaaagttgga aaggatacaa gtcaccatca ctttctttta atccaaactg ttgaaacaat
     4981 ccttcttgag tagccttagc attttctgag atacccaagc gcacctgatg cttgactttc
     5041 atagatggat ctaaataatt aacggattgt ggaatcaacg tcatttcttt tcctcgcaac
     5101 tgtttgatgc gtttagaagt tagtgattga ccacgataaa tcatatctcc tgttacagct
     5161 gcatttttag gaagaatatc cataatagca tgtgctaata aactcttgcc tgaaccacta
     5221 gcacctataa tagctaataa ctcacctttt ttaacttcta aattcagcgc ttggatcggt
     5281 gtggattgaa atggttttaa aaatcttccg tattgagtga aggtgatgga gaggtcttta
     5341 atgcttaata atgtttctgt catatctcta cctcctaaaa atgatccgtt tgagggtaaa
     5401 agagtttctt taaagattct ccgatagtat caaaggcatt gacaaccaaa ataagataaa
     5461 ggcctggaaa aatcaccaac caccaattgc caagagagat atgcttagct gcctctgaca
     5521 aaatgatacc aaccgaaggt tgttcggcag aaaggccaaa tcctaagaaa gtcatggatg
     5581 cttcatgcaa gatgacgtgc ggaaataaga ggataaaccc aatgaaaatt tgagaagcaa
     5641 tcaaaggcag gatatgatgc ctcacaatat aataaggcgt ttttcccatg cttttagaga
     5701 gctggacaaa ggctttattc tttagatcat agacttcatt gcggataagc cttgctagag
     5761 agggccaatg ggtaacagct gttgcaatga taaccccttg agcccctttc ccaacaacaa
     5821 aagaaatgag aatcataaaa atcaaatgag gcataccaat aaacaaatca accaaccagg
     5881 ctattatttt atcaataagg ctatttccta gacctgcaag cactccaaaa acggtcgcaa
     5941 gaaagactcc cataagggcg cctaataagc cgacttgtaa agagaaataa agccctttaa
     6001 tcgttctgac aaacatatcc ctacctaaac catctgtccc aaaaagatgg tttaaggaag
     6061 gagcgaggtt gcgtaaagct gcattggttt ccaaaggcgt cctatagaaa taaaggttta
     6121 aggctagaat actaagaatg agagaaatgg cgatacccag ttgccataaa accatcgttc
     6181 gacgtttcaa tatcatactt ttctccttaa ctgtggattg atgatgctat ttaaaatatc
     6241 cgcaataaga ttgcccgcaa aaacaaataa tgtccctatc atcacaatag ctagaaggag
     6301 cggtgtatca cttttaagtc ctgcttcagt gagggtagac cctaagccag gatatgagaa
     6361 aacttgctca gcaagaacag atcctccaaa caattctcca aaataggaaa aatgcagtgt
     6421 aatagctggt acgatagcat ttctaaggca atgatgttta aaaatttgcc actgtgtttc
     6481 cccacgcgct ctggcaaata agacatattc actagaaagc accgacatca ttttagttct
     6541 cgtatgaagg gtgacattgg caatgcctag aatacttagc gtgaaaacag gtaacataag
     6601 gtgcttaact cgatcagcta acgtaatatc ttgactcaaa gtgcctatcg gggaagaaat
     6661 accaatcggg aaccacccca gctggacaga aaagattaat aaaaaaatga gtccaatcca
     6721 aaacgttggt actgatatct gaaggtatga aaaccacctg acaactcggt caagtaattt
     6781 cccttgatgg aaagcggata acgttcctaa gataaatcca ataagacccg ataagatcca
     6841 agagagtccc ataagtatga aagaagcacc tgcccgtgat ctaataatat cactaacagg
     6901 ttgccgataa acgagcgagg tccctaaatc tccctgtatc acatttttca accaaataaa
     6961 atattggact agagctggct tatccaagcc atagtggtga gcaatcgctt tgtactgagc
     7021 aggggttagt gatgtgtcat aattgacact tgccatgact ggatctactg gagattgttt
     7081 taacaaaacg aaggtcaaaa cagatacccc aaaaataagc gtgacacatc tgatgatttt
     7141 ccagatgata ataatggtag tacgtttcac aatgaaatct ccttaatcga taagaaaagg
     7201 agccgaggaa acgatcagca cagttttgtg tatcttgttt tctcagactc caaacttaaa
     7261 gtcgctgtat taactaaacg cgttacttag ttgattcatc ccaagtccac tcggcaatgt
     7321 tagtcaataa tgaccaatca tgaccatgac tgtggacgcc ttgtttacct acattgatac
     7381 gtttatcacc aatataagta tggttaaggc tcaccaacca tacatttggc aaatctccaa
     7441 gagtagaagc acctgttttg ccatcccact gcgctaactt ccaatattcg ttagctttgt
     7501 caaggtcaga agatgtcatt gctttgtcaa ggtacttagt cacggtagga ttgttataaa
     7561 acgtaatatt ggtccaacct ttccctgcta ggcttggatg atgcgattca taaaattgct
     7621 gcgcgtgatg acgtcctccg gcataaagta aggctgagtc atgtgacttc gttgccattt
     7681 catcccagtt actagctttg agtttaatag taatccctag ggccttggct tgctctgcta
     7741 cttcaacggc taagttcgct cgcaattgat cattagtagg gtagtacaga tcaaacgctg
     7801 catcaaggtc acctttttta cggctaccgt ctgcttgttc tttccatccc gcttttgtca
     7861 ataattgctt agctttagct actttattat ctttaatggc tgtttttgga ttccaaaatg
     7921 gtgttttatc aataattgaa taagctggtt taccataacc atttaaaacc gtatcgagaa
     7981 ctttttgcct atttaaacca atagtcaagg cttttcggat tgctggatca ctagtgacat
     8041 catttcctac aggataacca tcaggagaat cagtgatgac gccctttttc acataaggta
     8101 atgataagcc gcgcacatca tttgatggaa tatcaaggag gcgggtgcct ttgacttttt
     8161 tatcagcaag ttctggcgtt gcgtagatca tatcaacatc accagattct aaagctgcta
     8221 gtgctgtgtt ttcatcaagt aagacccaag tccatttttt aaagtatggt tttttcccat
     8281 gccaataagg gttacgaaca aaaatagctt gttctccagc cttatattct tttaccatgt
     8341 aaggtcctga accgatagga ttgctcttat acttatcatt gtaatgtttt ttagggacga
     8401 ttgggatttc agtcaactgt gctgtaaatg tcgaatgcgc ctcagtcaaa tggatattga
     8461 cctgattttt cccaactact tcaacgttct taatgaaggt tagatcccaa gcctttccat
     8521 ctgctttcaa catatcataa gtaaacttaa catcatcagc agtaacaggc tcaccatttg
     8581 agaatttaaa atcatcatgc aagtcaaacg accaagtcag cccatcttca gagagatggt
     8641 atgttttagc aagctctcct tttatatcta gttcaggaga acgtttcaat agagtgctat
     8701 gagtgatatt cccttcattg tggactccat aacggtcctt tggatcgaat tcatgaggga
     8761 gctttgcccc catagaaacg acaagttcgt cttttggacg ttgtttgcgc tgacgttctt
     8821 ttgtttgagg cttttgttgt tgacatgcaa ctaaaataag cccagtcaaa aataacgtga
     8881 taatagagaa gtattttagg tattttgaca caatcaaatc tccttatttc taattaataa
     8941 ataataatag taacgactcc atctcattat agcaataaac aaataggagg gcaattgttt
     9001 aactggttaa ttagaatttt tgttattttc aggaagatac tcagggcttt tttataagtt
     9061 gatgtattaa caaaagataa agaggtttca tgacttattt tagattaaaa aaagaattaa
     9121 actggtcact aagaaataaa ggaattgtag tttacgaagg gaatcaaaaa cttcagatag
     9181 ccattaatac aggtaaaaat aacagtgaaa aagtcttttt tgatactttt cttgaggtgg
     9241 ttcgggcggc agcgactttc tccacagcaa aaaacatctg ttttggtctt ggcttacttc
     9301 gttcgcagaa acaggaaatc gggtatcaca ttagcagagt agttcatgtt ttttgagcat
     9361 cacactgggt ctgtctacgt tctaaaagct tttgtcattt ttgtatcata ccccctacaa
     9421 gtagaaacag cataataaag aaaatttata aacaaaaatg gctagtatta tcaaggaatt
     9481 tatcaatttt tgttttttaa aattaatata gaaagtatag cattctagaa caaatttaga
     9541 a
//
LOCUS       DAXWIQ010000054         9538 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000054 DAXWIQ010000000
VERSION     DAXWIQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.054"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(<1..615)
                     /locus_tag="ACGEUI_001515"
     CDS             complement(<1..615)
                     /locus_tag="ACGEUI_001515"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFF8166337.1"
                     /translation="MLVLYVSSKLIKRRRMKFMYSALLGNPVEHSISDELFEYLNHNI
                     GVLDEYKHKKILVDSVCLEDKIKHLINDPKCIGLNITLPYKREVMRYIDNLDRVAEIT
                     GSVNNIYKSGNIITGTNTDWKGIYNTLVFFNISHITKCCVLGTGGTARAAIFTLQKLQ
                     ISPTVVYREPISENTRSLIKSFPDLKYITYKELADQQILNDMEII"
     gene            complement(684..755)
                     /gene="shp3"
                     /locus_tag="ACGEUI_001516"
     CDS             complement(684..755)
                     /gene="shp3"
                     /locus_tag="ACGEUI_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP3"
                     /protein_id="HFF8166338.1"
                     /translation="MKKISKFLPILILAMDIIIIVGG"
     gene            835..1686
                     /gene="rgg3"
                     /locus_tag="ACGEUI_001517"
     CDS             835..1686
                     /gene="rgg3"
                     /locus_tag="ACGEUI_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Regulator gene of glucosyltransferase 3; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg3"
                     /protein_id="HFF8166339.1"
                     /translation="MKSKLGATLRKVRKGKQISLCFVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLILHDEDYTNSETFANLVQYIRKQYSSQNINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSSIIPSDDELLQLTDYLFKVEKWGYYEILLLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSRNKTNKRIVTQLAINCFILSINKEEFSNCSYLIDEIK
                     TLLDNELNFYEQTVFLYATGYFEFKRQLETGVEKMKQAIQVLDILNEDKLKLHYADHF
                     DKLVNKD"
     gene            complement(1939..5478)
                     /gene="smc"
                     /locus_tag="ACGEUI_001518"
     CDS             complement(1939..5478)
                     /gene="smc"
                     /locus_tag="ACGEUI_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFF8166340.1"
                     /translation="MFLKEIELEGFKSFADKTKIEFDKGVTAVVGPNGSGKSNITESL
                     RWALGESSAKNLRGGKMPDVIFAGTQNRNPLNYAKVAVVLDNSDHFIKTAKKEIRVER
                     HIYRNGDSDYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQIKLNQTQDNLDRLEDIIYELDTQLAPLEKQAKVAKQFLEL
                     DANRKQLQLDILVKDIDIAQERQTKDTEALAALQQDLASYYAKRQSMEEDYQKFKQKK
                     QVLSQESDQTQTTLLELTKLIADLEKQIELVKLESGQEAEKKAEAKKHLEQLQEQLDG
                     FQAEEKQCTEQLLHIDQQLCDVKQQLNELSNALERFSSDPDQLMETLREEFVLLMQKE
                     AALSNQLTALKAHLDKEKQARQHKAQEYQLLVTKLDQLNDESQKAQAHYKAQKEQVEM
                     LLQNYQEGDKRVQELERDYQLNQERLFDLLDQKKGKEARKASLESIQKSHSQFYAGVR
                     AVLQSQKKLGGIIGAVSEHLSFDSDYQTALEVALGANSQHIIVTDEAAAKRAIAYLKK
                     NRQGRATFLPLTTIKARSLSEHYHRQLATCEGYLGTAESLIRYDDSLSAIIQNLLSST
                     AIFETIDQANIAARLLGYKVRIVTLDGTELRPGGSFSGGANRQSNTTFIKPELEQISE
                     ELTRLVEQLKITEKEVAALQSDLIAKKEELTQLKLAGDQARLAEQRAQMAYQQLQEKQ
                     EDSKALLAALDQSQTTHSDESLLAEQARIEEALTAIAKKKNALTCDIDDIKENKDLIR
                     QKTQNIHQALSQARLQERDLLNEKKFEQANQSRLRTQLKQCQQNILKLESILNNNVSQ
                     DSIQRLPQWQKQLQDATEHKSGAQKRLVQLRFEIEDYEARLEETAEKITKESEKNDTF
                     IRRQTKLETHLEQVANRLRAYAKSLSEDFQMTLADAKEVTNSIDHLESAKEKLHHLQK
                     TIRALGPINSDAINQYEEVHERLTFLTSQKTDLTKAKNLLLETINSMDSEVKARFKVT
                     FEAIQKSFKETFTQMFGGGSADLVLTETDLLSAGIEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDFLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGITMQESGVSKIVSVKLKEAQEMTN"
     gene            complement(5479..6171)
                     /gene="rnc"
                     /locus_tag="ACGEUI_001519"
     CDS             complement(5479..6171)
                     /gene="rnc"
                     /locus_tag="ACGEUI_001519"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFF8166341.1"
                     /translation="MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNE
                     RLEFLGDAVLQLIISEYLFAKYPKKTEGDMSKLRSMIVREESLAGFSRFCSFDAYIKL
                     GKGEEKSGGRRRDTILGDLFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVKD
                     YKTCLQEFLQTKGDVAIDYQVISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQD
                     AAKNALAQLSEV"
     gene            complement(6615..7424)
                     /locus_tag="ACGEUI_001520"
     CDS             complement(6615..7424)
                     /locus_tag="ACGEUI_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8166342.1"
                     /translation="MNESGFKYSILASGSTGNCFYLETPKKRLLIDAGLTGKKITSLL
                     AEIDRKPEDLDAILITHEHSDHIKGVGVMARKYHLDIYANEKTWQLMDECNMLGKLDA
                     SQKHIFQRDKVLTFGDVDIESFGVSHDAIDPQFYRIMKDNKSFVMLTDTGYVSDRMTG
                     IIENADGYLIESNHDIEILRSGSYPWSLKQRILSDLGHLSNEDGAGAMIRSLGYNTKK
                     IYLGHLSKENNIKELAHMTMVNQLAMADLAVGTDFTVHDTSPDTACPLTDI"
     gene            complement(7428..8780)
                     /gene="vicK"
                     /locus_tag="ACGEUI_001521"
     CDS             complement(7428..8780)
                     /gene="vicK"
                     /locus_tag="ACGEUI_001521"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HFF8166343.1"
                     /translation="MTRDIIGNLSTFELAILILLVFVAFYFIHLAVRDYRNARIIRMM
                     SHKIRDLINGRYTDIIDEKADIELMELSDQLNDLSDVFRLTHENLAQEKNRLASILAY
                     MSDGVLATDRSGKIIMINETARKQLNLSKEEALKKNITDLLEGDTSYTYRDLVSKTPV
                     VTVNSRNDMGEFVSLRLRFALNRRESGFISGLVVVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALKEDIAPSFIKVSLDETNRMMRMISDLLNLSRIDNQVT
                     QLAVEMTNFTAFITSILNRFDLVKNQHTGTGKVYEIVRDYPITSVWIEIDNDKMTQVI
                     ENILNNAIKYSPDGGKITVRMKTTDTQLIISISDQGLGIPKTDLPLIFDRFYRVDKAR
                     SRAQGGTGLGLAIAKEIIKQHHGFIWAKSDYGKGSTFTIVLPYEKDAAIYEEWEEDVD
                     "
     gene            complement(8773..9483)
                     /gene="yycF"
                     /locus_tag="ACGEUI_001522"
     CDS             complement(8773..9483)
                     /gene="yycF"
                     /locus_tag="ACGEUI_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFF8166344.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYDIVTAFDGREAVTIFEEE
                     KPDLIILDLMLPELDGLEVAKEIRKTSHVPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTETIETAVAEENASSGTQELTIGNLQILPDAFVAKKHGQEVE
                     LTHREFELLHHLANHMGQVMTREHLLEIVWGYDYFGDVRTVDVTVRRLREKIEDTPSR
                     PEYILTRRGVGYYMKSYD"
BASE COUNT         2873 a         1926 c         1494 g         3245 t
ORIGIN      
        1 tatgatttcc atatcattta aaatttgttg atcagccaat tccttatatg taatatactt
       61 taaatcaggg aaactcttta tcaaacttct tgtattctca ctaattggct ccctgtatac
      121 aactgttggt gagatttgta gtttttgtaa agtgaaaata gcagctctag cagtaccgcc
      181 agtacctaaa acacaacatt ttgttatatg gctaatatta aagaaaacta atgtattata
      241 aatacctttc caatcagtat tagttcctgt aattatattt ccagatttat aaatattgtt
      301 aacagatcca gttatctctg ctactctgtc taaattgtca atatacctca ttacctctct
      361 tttataaggt aaagttatat ttaatccaat acattttgga tcatttatca gatgtttgat
      421 tttatcttct aaacacactg agtctaccaa aattttttta tgtttatatt catccagaac
      481 tcctatgttg tgatttagat attcaaataa ttcatctgat atagaatgtt caactggatt
      541 accaagcaat gcagaataca taaatttcat cctcctacgt ttaattagtt tactactaac
      601 ataaagtaca agcattacac agagctaacc attacctata agtctttaga tactctaaag
      661 acttaagtgt acacctaagt aacttaccca ccaacaataa tgataatatc cattgctaaa
      721 attaaaatcg gcaaaaattt tgaaattttc ttcataagcg gacttccttt cagtttttat
      781 ttattgtaca ataaaaaaaa acggaattga tttttgttgt gaaagtggga aaatatgaaa
      841 tcaaaacttg gtgccacact tagaaaagtt cgaaaaggaa agcaaattag tctgtgcttc
      901 gtggcagatg aacacctttc aaaatcacaa atctctcgtt ttgagcgcgg ggagtcagag
      961 atatcgtgta tccgactgat caatattcta gacaaattac acattacttt ggatgagttt
     1021 cttattttac atgatgaaga ctataccaat tccgaaacat ttgctaacct ggtacagtat
     1081 attcgtaagc aatattcatc acaaaatatc aataatatag catgtttact ttccgacact
     1141 tctgattaca ctctgaattc ttttgagaaa acaatggtaa agtccatttt acacacaatg
     1201 gattcaagca tcattccatc agatgatgaa ttacttcaac taacagatta tctctttaaa
     1261 gttgaaaaat ggggctacta tgaaatcctt ttgttgggta actgtgtacg aacaattaac
     1321 tacaactcct acttcctatt gacaaaggag atgttaaata attacattta ctcttcacgg
     1381 aacaaaacta ataaacgaat tgtcactcag cttgctataa attgtttcat tcttagtatc
     1441 aataaggagg aattttctaa ttgctcctat ttaattgatg agataaaaac cttgttagat
     1501 aatgaattaa acttctatga acagacagtt tttctctatg caacaggtta ctttgaattt
     1561 aagcgccaat tagaaactgg agttgaaaag atgaaacaag ccatacaagt tcttgatatt
     1621 ttaaatgaag acaagttaaa attacattac gctgaccatt ttgataaact agtcaacaaa
     1681 gattaagtaa aatcattctt taccttttaa cgaaacgcca atcaagggtg tctcaataaa
     1741 aaatcatcta ctctgacaac ttttctcttt tacctatcga cagctaaaaa gccttggaaa
     1801 ctgctatttc caaggctttc tttgtactct attgtatcaa ttagcttatt ccgatttctg
     1861 ttgctattag tttaatcatg aaatctccta acttatggcc gaacatccaa aaaaacagaa
     1921 ggaactccct tctgtttttt aattggtcat ctcttgagct tcttttaatt ttacagatac
     1981 aattttagag acccccgatt cctgcatggt aattccataa atactatctg ctgcagccat
     2041 tgttccttta cgatgggtca cgacaataaa ttgactatct ttgtcaaagc gattgaggaa
     2101 atccccaaag cgcttaacat ttgcttcatc aagagccgct tctacctcat ctaaaatcac
     2161 aaacggaatc gttttaacac gtataatagc aaacaataaa gccagtgctg acaaagcttt
     2221 ttctccgcct gacatcaagt taagagattg aattttctta ccaggaggct gcacagaaat
     2281 ctctatacca gcagacaaga ggtctgtttc cgttaaaacc aaatcagcag aacccccacc
     2341 aaacatctga gtaaaggttt ctttaaaact tttttgaatt gcctcaaaag tcactttgaa
     2401 acgagccttc acttcgctat ccatactatt aatagtctct agcaaaagat ttttagcttt
     2461 tgtcagatct gtcttttgac ttgttaaaaa agtcaaacgc tcatgcactt cctcatactg
     2521 attaatagca tcactattaa ttggtcctag tgcacgaata gtcttttgta aatggtgtaa
     2581 tttttctttg gcactttcta agtgatcaat actatttgtt acctcctttg cgtctgctag
     2641 tgtcatttga aaatcttctg ataaactttt agcataggct cgtaagcgat tagccacttg
     2701 ctctaaatgt gtctctaact tggtttgacg acgaataaat gtgtcatttt tttcgctttc
     2761 tttggttatt ttttctgctg tttcttctaa gcgggcctca tagtcctcta tttcaaatct
     2821 taattggact aaacgttttt gagcaccaga cttatgctct gtggcatctt gtaactgttt
     2881 ttgccactgg ggtagtcttt gaatgctgtc ttgacttacg ttattgttta gtatgctttc
     2941 aagtttgaga atattttgct ggcattgctt cagctgagtt cttagacgag attgattagc
     3001 ttgctcaaat ttcttttcat taagtaaatc tctttcttgc aaacgagctt gagataaagc
     3061 ttggtgaatg ttttgtgttt tttgcctaat caaatctttg ttttctttga tatcatcaat
     3121 atcacaagtt aaggcatttt ttttcttagc aatagctgtt aatgcttctt caatacgagc
     3181 ttgttctgcc aataaagatt catcagaatg agtcgtctga ctttggtcaa gggcagctaa
     3241 caaagccttt gaatcttctt gtttttcttg caattgctga taagccattt gagctctttg
     3301 ttcagctaag cgtgcctggt caccggctaa tttcaattgt gtcagttctt ctttttttgc
     3361 tatcaaatcg ctttgcaagg cagctacttc tttttcagtt atttttaatt gctcaactaa
     3421 tcgcgttaat tcctcactga tttgttctaa ctctggtttg ataaaagttg tattgctttg
     3481 tctattagct cctcctgaaa aagaaccacc tggtcgtaat tctgttccat caagagtaac
     3541 aattcttacc ttatatccta ataagcgcgc tgcaatatta gcttgatcaa tagtttcaaa
     3601 gatagcagta ctagataaaa gattttggat aatagctgaa agactatcat catagcgaat
     3661 caatgattct gcagttccca gataaccttc acaagtcgcc aattgacgat gatagtgttc
     3721 tgataatgat ctagccttta tagtagttaa aggtaaaaaa gttgctcgtc cttggcgatt
     3781 ttttttcaaa taagcaattg ctctttttgc tgcagcttcg tctgttacaa taatatgctg
     3841 actattagca cctaatgcaa cttcaagagc tgtttggtaa tcagaatcaa aagaaaggtg
     3901 ttcactgact gcacctatga tccctccaag ctttttttgt gattgaagga cagctcgtac
     3961 tcccgcatag aattgactat gacttttttg aatagattct aagctagcct tacgagcttc
     4021 ttttcctttt ttttgatcca acaagtcaaa tagtctttct tgattcagtt ggtaatctct
     4081 ttctaattcc tgaacgcgtt tatcaccttc ttgataattt tggagcaaca tttcgacttg
     4141 ctctttttga gctttataat gagcttgagc tttttgagac tcgtcattaa gttgatcaag
     4201 cttagtaaca agtagctgat attcttgagc cttatgttga cgagcctgtt tttctttgtc
     4261 taaatgggct tttaaagctg tcagctgatt tgataaggcc gcctcttttt gcatcaataa
     4321 tacaaactct tctcttagag tttccattaa ttggtcggga tcacttgaaa aacgctctaa
     4381 tgcattgctc aattcgttta actgttgttt gacatcacaa agttgttgat caatatgcaa
     4441 taattgttct gtacactgct tttcttctgc ttgaaaacca tccaattgtt cttgtaactg
     4501 ttcgagatgt tttttggctt ctgccttttt ctctgcttct tggccacttt ctagcttaac
     4561 caattcaatt tgcttttcca agtcagctat tagcttggtt aattctaata atgttgtttg
     4621 ggtttgatct gactcttgac taagaacctg ctttttttgc ttgaattttt ggtaatcttc
     4681 ttccatagat tgacgcttag cgtaataaga tgctaagtct tgttgtaaag cagctaaagc
     4741 ttcagtatct ttagtctgtc tttcttgagc tatatcaata tctttgacta gaatatctag
     4801 ttgtagttgc ttacgattgg catcaagttc taaaaattgt ttagctactt tagcttgctt
     4861 ttcaagtggt gccaactgcg tgtctagctc ataaataata tcctctagac ggtctaaatt
     4921 atcttgagtt tgatttaatt taatctgagt ttctttttta cgtgttttat attttaaaac
     4981 accagctgct tcttcaaaaa tggcgcgcct ttcttcaggt ttactattga aaatttcctc
     5041 tacgcgacct tgagaaataa tggagaaaga atcacgacca agccctgtat ccataaacaa
     5101 atcatgaata tcacggagcc ttaccttgcg accatcaatc agatagtcac tatctccatt
     5161 tcggtaaata tgcctttcga cacggatttc tttcttggca gtttttataa aatgatcgga
     5221 attatcaaga acaaccgcta ccttagcata gtttagagga ttacggtttt gtgtcccagc
     5281 aaaaatgaca tctggcatct tgcccccacg cagattttta gcacttgatt ctcctaaagc
     5341 ccaacgtaaa ctttctgtaa tattactttt acctgaacca tttggtccta cgactgcagt
     5401 tactccctta tcaaattcaa ttttagtctt gtccgcaaag gacttgaacc cttccaactc
     5461 aatttctttt aaaaacatct aaacctcact aagttgggca agggcatttt tagcagcatc
     5521 ttgctccgct aattttttag acttacctaa acctttacta aggacagcac cattaacaac
     5581 tatcgatacc tcaaattgtt tagcatgggc aggtcctttc tcactaatga cttgataatc
     5641 aatagccaca tcaccttttg tttgcaaaaa ctcttgaaga caagttttat agtctttaac
     5701 cctttcaaaa ttcccttttt ccacctgagg aatcatgact tgtttcaaga agcgacgtac
     5761 tgcatcaatc cccttatcta ataaaagagc acctaaaaag gcttcaaata agtctcctaa
     5821 aatagtatca cgacggcgac cacctgactt ttcctcacct tttcctagtt tgatataagc
     5881 gtcaaaagaa caaaatcgtg aaaaacctgc caagctttcc tcacgtacaa tcatggaacg
     5941 taacttagac atatctcctt ctgtcttttt aggatacttt gcaaataaat actctgaaat
     6001 aatcaattgt agaacagcgt ctcctaaaaa ttccaagcgt tcattatgtg aaacgtttag
     6061 gagacgatgc tcgttagcgt aagaggtgtg ggtaaaagct gtttctagta aagtcaaatc
     6121 attaaattga atatcaaaag atgttgagag taactcttca agctgtttca taaaaaatcc
     6181 ctttcatatt tgtcagtaga ccttatttag tataccaaaa atccacctag aaaaccgact
     6241 ctaagtggat ttttcgtgat ttattaaaca tcttaaatac ccgtcaaagg aaactttctc
     6301 atacaatcaa aagactctac actatctgag gggaatttcc cactacagtc ccactacaga
     6361 tagtatagag ctatttttgt ttttagtctg aacaactaaa aaactacctc atctttataa
     6421 tcctcaacac ctttgataaa tagatcagac aagccaaaac tacaggctaa tttaagtcta
     6481 ttgatgctct aaaggatgac caatgagagt tcgttgaact cagaagacaa tacgctttaa
     6541 gacatcaaaa aatagccctg actggtatat caccaatcag cgctactttg catattctct
     6601 tttagtttct tatctcaaat atcagttaat ggacaagcag tatctggaga ggtatcatgg
     6661 accgtaaagt ctgtacctac tgctaaatct gccatagcca gttgattgac catcgtcata
     6721 tgcgctaact ctttgatgtt attttcttta ctcagatgac ctaggtatat ttttttggta
     6781 ttgtacccca ggcttctaat catggctccc gctccatctt catttgataa atgtcctaaa
     6841 tcagataaaa tacgctgctt taagctccaa ggataagagc ctgatctcaa aatttcaata
     6901 tcatgattgg attcaatcaa gtagccatct gcattttcaa taatacctgt catcctatca
     6961 ctgacatatc ctgtatctgt caacatcaca aaagacttat tatctttcat aatccgataa
     7021 aattgaggat caatagcgtc atggctgact ccaaaacttt cgatatcaac atctccaaag
     7081 gtcaaaactt tatccctttg gaaaatatgt ttttgtgagg catcaagctt acctagcata
     7141 ttgcactcat ccatcagctg ccatgttttt tcattagcat agatatccaa atggtatttg
     7201 cgggccataa cgcctacacc cttaatatgg tcagagtgct catgggtaat caaaatagca
     7261 tccaaatcct caggtttgcg gtcaatttca gcaagaagac tggtaatttt cttaccagta
     7321 agccctgcat caatcaataa gcgtttttta ggtgtttcta aataaaaaca atttcctgtg
     7381 gacccagatg ctaaaatact gtatttaaaa ccactctcat tcattaatta gtctacatct
     7441 tcctcccatt cttcatagat ggctgcatct ttttcataag gcaagacaat agtaaaggtc
     7501 gatcctttac catagtcact cttagcccag ataaagccat ggtgctgctt gatgatttct
     7561 ttagcaatgg caaggcctag accggtccct ccttgggcac gacttcttgc cttgtctaca
     7621 cgatagaacc gatcaaaaat aagaggcaaa tctgttttag ggatacctag tccttggtct
     7681 gaaatggaaa taattaattg ggtatctgtt gttttcatac ggactgtaat ttttccacca
     7741 tctggagaat acttaatggc attgttcaaa atattctcga taacctgtgt cattttatca
     7801 ttatcaattt caatccagac agaggtaata gggtaatctc ttacaatttc atagactttt
     7861 cctgtacctg tatgctgatt tttaaccaaa tcaaatctgt ttaaaataga agttataaaa
     7921 gcagtaaaat tagtcatctc tactgctaat tgggttactt gattatcaat ccgagacagg
     7981 tttaaaagat ctgaaatcat acgcatcatc cgattagttt catcaagaga aacttttatg
     8041 aaacttggag caatatcttc tttaagtgca ccttcatcaa gagcctccaa gtaggattta
     8101 accgaagtta aaggggtcct taattcatga cttacattag agacaaaaag acggcgttca
     8161 cgttcttctt tttcctgttc tgtggtgtca tggagcacca caaccaagcc cgaaataaaa
     8221 ccactctctc tcctattcaa cgcaaagcgc aagcgtaatg agacaaactc acccatatca
     8281 tttcggctat taacagttac cactggtgtt ttggatacca aatcacggta ggtatatgaa
     8341 gtatcacctt ctaacaaatc tgtaatgttt ttctttagtg cctcttcttt acttaaattt
     8401 aattgcttgc gagctgtctc gttaatcatg atgattttac cagaccggtc tgtagcaagt
     8461 acaccatctg acatataagc caaaatactt gccaagcgat ttttttcttg ggcaagattt
     8521 tcatgcgtca agcgaaaaac atctgacagg tcatttaact ggtctgaaag ctccattaac
     8581 tcaatgtctg ctttttcgtc gattatatca gtatagcgac cattaatcaa gtctcggatt
     8641 ttatggctca tcatccgaat aatacgtgca tttcggtaat cacgcaccgc aagatggata
     8701 aagtaaaaag caacaaaaac aagtaagata aggatcgcta attcaaatgt ggataagttt
     8761 ccaatgatat ctctagtcat aagatttcat gtagtaccca acaccacgtc ttgttaaaat
     8821 atactcagga cgacttggtg tgtcttcaat tttttcacgg aggcgacgaa cagtcacatc
     8881 aaccgtgcgc acatcgccaa aataatcata tccccaaaca atttccaata agtgttctcg
     8941 tgtcatcacc tgccccatat ggttagctag atgatgcaat agttcaaatt cacgatgggt
     9001 caactctacc tcttgaccat gttttttagc aacaaacgca tctggtaaaa tctgtaaatt
     9061 accaatggtc agttcctgtg tacctgaaga agcattttct tctgcaacag ccgtttcaat
     9121 agtttcggta cgacgcagat gagccttgac acgcgccagc aattcccgat tagaaaaggg
     9181 cttggtcacg taatcatcag ccccaatttc aagtccaata accttgtcaa actcactatc
     9241 tttagccgac aacataataa tcgggacatg actggtttta cgaatttcct tggctacttc
     9301 aagaccgtcc aactcaggga gcatcaaatc aagaataatt aaatctggct tttcttcttc
     9361 aaaaattgtt accgcttcgc gtccatcaaa agctgtaaca atgtcataac cttcttttgt
     9421 caaattaaac ttaataatgt cagaaatcgg tttttcatca tccacaataa gtattttttt
     9481 catgtctata cctcagaaag ttcttgtaat tgatattaca atttgtgatt tttgtggt
//
LOCUS       DAXWIQ010000055         9334 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000055 DAXWIQ010000000
VERSION     DAXWIQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.055"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(105..1364)
                     /locus_tag="ACGEUI_001523"
     CDS             complement(105..1364)
                     /locus_tag="ACGEUI_001523"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFF8166345.1"
                     /translation="MRKIIINGGKALSGEVAVSGAKNSVVALIPAIILADDIVILDGV
                     PAISDVDSLIEIMELMGATVNYHGDTLEIDPRGVQDIPMPYGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFEAMGVEVSYEGENMNLSTNGQKIHGAHIYM
                     DTVSVGATINTMVAATKAQGKTVIENAAREPEIIDVATLLNNMGAHIRGAGTDIITIQ
                     GVQKLHGTRHQVIPDRIEAGTYIALAAAIGKGVKITNVLYEHLESFIAKLEEMGVRMT
                     VEEDAIFVEKQESLKAITIKTSPYPGFATDLQQPLTPLLLKADGRGTIIDTIYEKRIN
                     HVPELMRMGADISVIGGQIVYQGPSRLTGAQVKATDLRAGAALVTAGLIAEGKTEITN
                     IEFILRGYASIIAKLTALGADIQLIED"
     gene            complement(1538..2734)
                     /gene="metK"
                     /locus_tag="ACGEUI_001524"
     CDS             complement(1538..2734)
                     /gene="metK"
                     /locus_tag="ACGEUI_001524"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFF8166346.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAE
                     TCVYTGSVHVFGEISTTAYIDINRVVRDTIAEIGYTEAEYGFSAESVGVHPSLVEQSG
                     DIAQGVNEAFESREGDTDDLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLA
                     ELRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEATNDQIRQDVIEKV
                     IKTVIPADYLDDDTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSG
                     KDATKVDRSASYAARYIAKNLVAAGLVTKAEVQLAYAIGVAQPVSVRVDTFGTSTVPE
                     AVLEAAVRQVFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWERLNKVDALVE
                     AVKTVL"
     gene            complement(3271..5649)
                     /locus_tag="ACGEUI_001525"
     CDS             complement(3271..5649)
                     /locus_tag="ACGEUI_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFF8166347.1"
                     /translation="MKTKKVIILVGLLLSSQLTLIACQSRGNGTYPIKTKQSRKGMTS
                     NKIKPIKKSKKTNKTHKGVAGVDFPTDDGFILTKDSKILSKTDQGIVVDHDGHSHFIF
                     YADLKGSPFEYLIPKGASLAKPAVAQRAASQGTSKVADPHHHYEFNPADIVAEDALGY
                     TVRHDDHFHYILKSSLSGQTQAQAKQVATRLPQTSSLVSTATANGIPGLHFPTSDGFQ
                     FNGQGIVGVTKDSILVDHDGHLHPISFADLRQGGWAHVADQYDPAKKAEKPAETHQTP
                     ELSEREKEYQEKLAYLAEKLGIDPSTIKRVETQDGKLGLEYPHHDHAHVLMLSDIEIG
                     KDIPDPHAIEHARELEKHKVGMDTLRALGFDEEVILDIVRTHDAPTPFPSNEKDPNMM
                     KEWLATVIKLDLGSRKDPLQRKGLSLLPNLETLGIGFTPIKDISPVLQFKKLKQLLMT
                     KTGVTDYRFLDNMPQLEGIDISQNNLKDISFLSKYKNLTLVAAADNGIEDIRPLGQLP
                     NLKFLVLSNNKISDLSPLASLHQLQELHIDNNQITDLSPVSHKESLTVVDLSRNADVD
                     LATLQAPKLETLMVNDTKVSHLDFLKNNPNLSSLSINRAQLQSLEGIEASSVIVRVEA
                     EGNQIKSLVLKDKQGSLTFLDVTGNQLTSLEGVNNFTALDILSVSKNQLTNVNLSKPN
                     KTVTNIDISHNNISLADLKLNEQHIPEAIAKNFPAVYEGSMVGNGTAEEKAAMATKAK
                     ESAQEASESHDYNHNHTYEDEEGHAHEHRDKDDHDHEHEDENEAKDEQNHAD"
     gene            5853..6794
                     /gene="birA"
                     /locus_tag="ACGEUI_001526"
     CDS             5853..6794
                     /gene="birA"
                     /locus_tag="ACGEUI_001526"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFF8166348.1"
                     /translation="MKTSEKIYQLLSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQ
                     GIQIDSLKHKGYRMVQGDILLPKTISQGLGMPVTYTPHSQSTQLDAKQGIEAHNSAPR
                     LYLAPSQEAAKGRLDRQFFSASTGGIYMSMYLKPNVPYADMPPYTMMVASSIVKAISR
                     LTGIDTEIKWVNDIYLGNHKVAGILTEAITSVETGLITDVIIGVGLNFFVTDFPEAIA
                     QKAGSLFTEKPTITRNDLIIDIWKLFLSIPVKDHVKVYKEKSLVLNKQVTFIENSQEK
                     RAIAIDLTDQGHLIVQFENGDLQTLRSGEISLSSWEN"
     gene            complement(6769..6972)
                     /locus_tag="ACGEUI_001527"
     CDS             complement(6769..6972)
                     /locus_tag="ACGEUI_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 family protein"
                     /protein_id="HFF8166349.1"
                     /translation="MTIRQFLFMAFVCAFETYFFNDLLLSGNYLFALFWGLLLFRDLR
                     RVHTINQLTKTILKTANSPKKKD"
     gene            complement(7067..8737)
                     /gene="dnaX"
                     /locus_tag="ACGEUI_001528"
     CDS             complement(7067..8737)
                     /gene="dnaX"
                     /locus_tag="ACGEUI_001528"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFF8166350.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVESGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVDGEPCNQCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKQKAIREHLAWVLDKEGIAYEVDALNLIARRAEGGMRDALS
                     ILDQALSLSPDNQVAIAIAEEITGSISILALGDYVRYVSQEQATQALAALETIYDSGK
                     SMSRFATDLLTYLRDLLVVKAGGDNQRQSAVFDTNLSLSIDRIFQMITVVTSHLPEIK
                     KGTHPRIYAEMMTIQLAQKEQILSQVNLSGELISEIETLKNELAQLKQQLSQLQSRPD
                     SLARSDKTKPKTTSYRVDRVTILKIMEETVRNSQQSRQYLDALKNAWNEILDNISAQD
                     RALLMGSEPVLANSENAILAFEAAFNAEQVMSRNNLNDMFGNIMSKAAGFSPNILAVP
                     RTDFQHIRKEFAQQMKSQKDSVQEEQEVALDIPEGFDFLLDKINTIDD"
     gene            complement(8737..9234)
                     /locus_tag="ACGEUI_001529"
     CDS             complement(8737..9234)
                     /locus_tag="ACGEUI_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFF8166351.1"
                     /translation="MNKSKKIEQYQLMIAQAKELFANESNALANLSNASALLNMTLPN
                     SVFTGFYLFDGQELILGPFQGRVSCVHIKLGKGVCGESAQSRRTIIINDVKQHANYIS
                     CDAAAMSEIVVPMVKEGHLIGVLDLDSSLVADYDEVDQEYLEAFVDLFLEKTTFTFNM
                     FGVKN"
BASE COUNT         2750 a         1995 c         1681 g         2908 t
ORIGIN      
        1 cttttttatg acagttaaaa agccataaaa tgcaacaaag tatgcaccta agtatcatta
       61 gcctaagcac atgtaatata aaaaagagaa gcgttagctt ctctttagtc ttcgattaat
      121 tgaatatctg cccctagtgc tgttaatttt gcaatgatac tagcatagcc acgtaagata
      181 aattcaatat tagtaatttc tgtcttgcct tcagctatta gaccagcagt cactagagcc
      241 gctccggcac gtaaatcagt ggctttgacc tgagctcctg tcaggcgaga cggcccttga
      301 taaacaattt gaccaccaat aactgagata tctgctccca tgcgcatcaa ctctggcaca
      361 tggttaatgc gcttttcata tatagtgtca ataatcgttc cacgaccatc tgcttttaac
      421 aacaagggcg ttaatggctg ttgcaaatca gtggcaaaac cagggtaagg agaggtttta
      481 attgtaatag ccttgaggga ttcttgcttt tcaacaaaga tagcatcctc ttctaccgtc
      541 atacggacac ccatttcttc taacttagca atgaaactct ctaaatgctc ataaagaaca
      601 ttagtgatct taacaccttt tccaatagct gccgccaaag cgatataagt ccctgcttca
      661 atacgatcag gaataacttg gtgtctagta ccatgcaatt tctgaacacc ttgtatcgta
      721 ataatatctg tacctgctcc acgaatatgc gcacccatgt tattcaaaag agtagccaca
      781 tcaataatct ctggctcacg tgcagcgttt tcaataaccg ttttaccttg agcttttgtt
      841 gccgcaacca tagtattaat tgtagcaccg acactaacgg tgtccatata gatatgagcg
      901 ccatgaattt tttgaccatt tgtcgataaa ttcatatttt caccctcata agacacttca
      961 actcccatag cctcgaaggc cttcaaatgc aagtcaattg gtcttggtcc taaatcgcaa
     1021 ccaccaggta agccaacaac tgcttgtcca aaacgaccta aaagactacc gtagaaataa
     1081 taggaagctc gaagactatt aatctttccg taaggcatcg ggatgtcttg cacccctcta
     1141 gggtcaattt ctaaagtatc accatgatag tttactgttg ctcccataag ctccatgatt
     1201 tcaatcagac tatctacatc actaatagca ggtacaccat caagaatcac aatatcatcg
     1261 gctaagatga ttgctggaat aagcgcaaca acactgtttt ttgcccctga aactgccact
     1321 tctccagata aggcttttcc gccattaata ataattttac gcatacattc aaaactttct
     1381 tatactagaa ttcaaccttt tcattttatc ataaatcatg aagcctgaca attattgacg
     1441 tcttaatttt taatcatccc ttttaaaggc ttatttaata acaaaaaagc agagtaagat
     1501 tttttcactt actctacttt tttaataaag ttgtcgttta taatactgtt tttacagctt
     1561 ctactaaagc atcaacctta tttaaacgct cccatggcaa atcaatatct gttctcccca
     1621 tgtgaccata ggcagctgtt tgtttgtaaa tcgggcgttt taaatcaagc atttgaataa
     1681 tgcctgctgg ccgtaaatca aagacttgac gaacagcagc ttctaaaaca gcttcaggaa
     1741 ccgttgaggt tccaaatgta tccacgcgaa cagacacagg ctgagccacc ccaatcgcat
     1801 aagctagttg gacttcagct ttggtcacaa ggcctgctgc cacaaggttt ttagcaatat
     1861 agcgagcagc gtaagaagcc gaacggtcca cttttgtcgc atcttttcct gaaaaagcac
     1921 cgccaccatg acgtgaatag cctccatagg tatccacaat aatcttacgt ccggtcaatc
     1981 cagagtctcc ttgcggacct ccaataacaa aacgtcctgt tggattaatg aagaactttg
     2041 tgtcatcatc taaataatct gctggaatta cagtcttgat cactttttca atcacatcct
     2101 gacgaatttg gtcattagta gcttctggat catgctgagt agaaataacg actgtatcca
     2161 cacgaacagg tttatcgtgt tcatcgtact caactgtcac ttgagattta gcatctggac
     2221 gtagataact aatctcacct gacttacgta attccgccaa gcgacgcact aattggtgag
     2281 ataaggaaat tggtaaaggc atcaattctg gtgtttcatt gatggcaaaa ccaaacatga
     2341 gcccttggtc acccgctcca atatggctaa ggtcatcagt atcaccttct ctgctttcga
     2401 aagcttcatt aaccccctga gcgatatctc cagattgttc tacaagtgat ggatgtactc
     2461 ccactgattc tgctgaaaag ccatattctg cttctgtata accaatttct gcaatggtgt
     2521 cgcggaccac acggttaata tcaatgtatg ctgttgttga gatttctcca aaaacatgaa
     2581 cagaacctgt gtaaacacaa gtttctgcag ccacatgggc ttctggatct tctgctaaaa
     2641 tggcatctaa aatagcatct gaaatttggt ctgcaatctt atctggatgc ccttcagata
     2701 cggactcaga cgtgaaaagt ttacgttctg acataaaaat gtccccccta atatgttgat
     2761 tcttcctttt tcagtcacat tgaccacaaa gcaagaagtt gcctacccct ttcttcctag
     2821 ctgattgagc aagtcaagtg gccaaaacca acttagctaa tcagttacgg ttttactagt
     2881 aaggcaacta gctagtccac ctagtggcta gcgtccatta accagacaaa gaatgtctga
     2941 caatagcgtt gagttacaaa gaatccggtc tccttgttga gaggatgtca ccacaaaata
     3001 agccttagac gaagtaattt agctaaaggg agaacgactg ttccccaaat gaagtcaaca
     3061 acgtctcaaa aacatttcgt tcaacagacc gtcttatcgg gactcaataa caatctatta
     3121 taacatgttt tagccattta ggtgccattc ttttcactca ttatcctatt tatttcaaaa
     3181 aaacctcaag gttattcttg aggttctctt gatagtcaac aatggtgtca ttaggacgat
     3241 tcagttggat cgggttattc ttagattagt ttagtcagca tggttttgct catctttagc
     3301 ttcattttca tcctcatgtt catggtcgtg atcatctttg tctctgtgct cgtgagcatg
     3361 accttcttca tcttcatagg tatgattatg gttgtagtca tgtgattccg atgcttcttg
     3421 agcactttct ttcgccttag tagccatagc tgctttttct tcagctgttc cattacctac
     3481 catagaacct tcgtaaaccg ctgggaagtt tttcgcaatg gcttctggaa tatgttgctc
     3541 gttcaattta aggtctgcta atgagatatt gttatgacta atatcaatgt tagtaactgt
     3601 cttattgggt ttagataggt tgacatttgt taattggttt ttagacacgc ttaaaatgtc
     3661 aagtgctgta aaattattaa caccttctag agaagtcaac tggttgcctg tcacatccaa
     3721 gaaagtaagt gacccttgct tgtctttaag cacaagcgat ttaatttggt taccttctgc
     3781 ttctactctg acaatgacgc tacttgcttc aataccttca agagattgca attgcgcacg
     3841 gttaatagat aggctagata gattaggatt atttttcaag aaatccaaat gagaaacctt
     3901 ggtatcattg accattaacg tttctaattt gggtgcttga agtgttgcta agtcaacatc
     3961 agcatttctt gataaatcaa caaccgtcaa tgattcttta tgagaaacag ggcttaaatc
     4021 tgtaatctga ttattatcaa tgtgcaattc ttgcaattga tgtaacgatg ccagtgggct
     4081 taaatcagaa atcttattgt tactcaatac gaggaatttg agatttggta attgaccaag
     4141 cggcctaata tcttcaatac cattatcagc agccgctact agagttaagt ttttatattt
     4201 gctcaagaaa ctaatatctt tgagattgtt ttgtgaaata tcaatgcctt ctaactgtgg
     4261 catattatcc aaaaatctat aatcagtcac ccctgttttt gtcattaaca actgtttcaa
     4321 ttttttaaat tgcaaaacag gtgagatatc tttgattggt gtaaagccaa ttcctaaagt
     4381 ttctaagttg ggtaacagtg aaagtccttt acgttgcaaa ggatctttac ggctgcccaa
     4441 gtcaagtttg ataaccgttg ctaaccattc tttcatcata ttcggatctt tttcatttga
     4501 tgggaatggg gttggagcat cgtgagtgcg aacgatatcc aaaatcactt cttcatcaaa
     4561 ccctaaggca cgcaaggtat ccattccaac cttatgtttt tccaattcac gggcatgctc
     4621 aatagcatgt ggatctggaa tgtcttttcc gatttcaata tcagataaca tcaatacgtg
     4681 tgcgtggtca tggtgagggt attccaaacc aagtttaccg tcttgtgttt ccacacgttt
     4741 aatagttgat ggatcaatcc ccaatttttc tgccaaataa gctaattttt cttggtattc
     4801 cttttcacgt tcagatagct ctggtgtctg atgggtttct gctggctttt ctgctttttt
     4861 agcgggatcg tattgatctg ccacatgtgc ccagccaccc tgacgaaggt ccgcaaaaga
     4921 aataggatgt aagtgaccat cgtggtccac taaaatactg tcttttgtta ccccaacaat
     4981 accttgaccg ttaaattgaa aaccatctga ggttgggaaa tgcaagcctg gaataccatt
     5041 agctgtagct gttgaaacaa ggctactggt ttgtggcaag cgagtagcaa cctgtttagc
     5101 ttgtgcctgt gtctgacctg ataagcttga cttcaaaata taatggaagt gatcatcgtg
     5161 gcgaaccgtg tagcctaaag catcttcagc cacaatatcc gctgggttaa attcataatg
     5221 gtgatgagga tctgctactt tagaagtccc ttgactagct gctcgctgag caacagctgg
     5281 cttagctaaa cttgctcctt ttggaataag gtattcaaat ggacttccct ttaaatcggc
     5341 ataaaaaata aaatgcgaat gaccatcatg gtcaacaacg attccctgat ctgtttttga
     5401 taagattttt gagtctttgg ttaaaataaa cccatcatct gtaggaaaat cgacacccgc
     5461 cacacctttg tgagtcttgt ttgtcttttt gcttttttta atcggtttaa ttttgtttga
     5521 cgtcattccc ttacgtgatt gtttcgtttt aatgggatat gtaccattac ctcgtgattg
     5581 acaagctatc aaagtcaact gagatgataa caatagacca actaaaataa taactttttt
     5641 cgttttcatt caaccctcct ttttatttaa ctggttaagt atatcacatc acttgttttt
     5701 cctcaagctc tttttaggat aactattagt tgttgacgag ttggagctct gaagcttcaa
     5761 caaacaatag tttctgttgt tagtagcatt acacaaaaaa actaactttt cttttccttt
     5821 gaaaataaga tcttttataa tatactatac atatgaaaac atcagaaaaa atttatcaat
     5881 tactgtcaca aaccgatgac ttcgtcagtg gggaatatct agcagatcaa ctcagtatct
     5941 ctcgtacatc cgtttggaaa tccatcaaaa gtctcgaaaa tcaggggatt caaattgatt
     6001 ctttaaaaca caagggctac cgcatggtac aaggtgatat tttattaccc aaaaccatta
     6061 gccaagggct tgggatgcca gtcacctata cccctcacag ccaatctacc caattagatg
     6121 ccaaacaagg aatcgaagct cataacagcg ctccaaggct ttacctagct cctagccaag
     6181 aagctgccaa aggacgcctc gatcggcaat ttttttcagc tagcaccggt ggtatttaca
     6241 tgtccatgta cctaaaaccc aatgtccctt atgctgatat gcctccttat acgatgatgg
     6301 ttgcttccag tattgtcaaa gctatctcgc gattaacagg cattgataca gaaatcaaat
     6361 gggtaaacga catctatcta ggtaaccata aagttgcagg gattctgaca gaagccatta
     6421 cttctgttga aactggttta atcaccgatg tcattattgg ggttggtctc aacttttttg
     6481 tcaccgactt cccagaagcg attgctcaaa aggcaggttc tctctttacc gaaaaaccga
     6541 ctatcacgcg caatgacctt atcattgaca tctggaaatt attcttatct attcctgtca
     6601 aggaccatgt caaagtttac aaagaaaaat cacttgttct taataaacaa gtgaccttta
     6661 tagaaaatag tcaagaaaaa cgtgctattg cgattgatct cacagatcaa ggacacctca
     6721 ttgtacagtt tgaaaatggt gaccttcaaa ccttgcgaag cggtgaaatc agtctttctt
     6781 cttgggagaa ttagccgttt ttaaaatggt tttagtcagt tggttaatgg tatgcactct
     6841 tcttaaatcc ctaaagagca acaagcccca aaataaggcg aagagataat ttccagagag
     6901 taacaggtca ttaaaaaaat aggtttcaaa ggcacataca aaggccataa aaagaaattg
     6961 tcgaatagtc atagctttat tataacaaaa aaatactgta ctatgaagaa aagtaacgag
     7021 ataaaacgtg ggtaagggca aaaccttacc cttatgtatt tcagttttag tcgtcaatag
     7081 tatttatttt atcgagcaaa aaatcaaacc cttctggaat atcaagcgct acttcttgtt
     7141 cttcttgaac actgtctttt tgcgatttca tttgctgagc aaattcctta cgaatatgct
     7201 gaaaatctgt ccttggtact gccagaatat tgggagaaaa accagcagct ttactcataa
     7261 tgttaccaaa catatcatta agattatttc ggctcatgac ttgttctgca ttaaaggcag
     7321 cctcgaaagc caaaatcgca ttctcactat ttgctaagac aggctcagag cccatcaata
     7381 aggctctgtc ttgggcagaa atgttatcta gaatttcatt ccaagcattt tttagagcat
     7441 ctagatattg tcgagattgt tggctatttc gaaccgtttc ttccatgatt ttcaaaatgg
     7501 taacccgatc aaccctgtag cttgtggttt taggtttcgt tttatcagat cttgccagtg
     7561 aatcaggacg cgattggagc tgcgacaatt gttgtttaag ttgtgccaac tcatttttga
     7621 gcgtttcaat ctctgagatt aactctcctg acaagtttac ttgggacaaa atctgctctt
     7681 tctgagctaa ttggatagtc atcatttcgg cataaatccg aggatgggtt ccctttttga
     7741 tttcagggag atgactagta acaactgtta tcatttggaa tatacgatct atcgagagag
     7801 acaaattggt atcaaaaaca gctgactgac gttgattgtc gccgccagct ttaaccacca
     7861 ataaatcacg cagataggtc aataaatctg tcgcaaagcg gctcatgctc ttcccactat
     7921 cataaatggt ctctaaggct gccagagctt gcgtagcctg ttcttgggag acatatcgaa
     7981 catagtcacc cagagcaagt atggaaatag aacctgtaat ttcttcggca attgcaatgg
     8041 cgacctgatt atctggtgac aagctcaaag cctgatctaa aatagataaa gcatcacgca
     8101 tgcctccttc tgctcgcctt gcaatgagat ttaaagcatc cacctcatag gcaatacctt
     8161 ctttgtccaa aacccaggct aaatgctctc gaatagcttt ttgcttaata gctttgaatt
     8221 caaagcgttg cacacgagat aaaatagtgg ctggaatttt atgcaattcc gttgttgcca
     8281 agataaagac aacattttct gtcggttctt ccaaagtttt caaaagcgca ttaaaagccc
     8341 ctgttgataa catgtgaacc tcatcaataa tataaacctt gtaagtcgca cgacttggcg
     8401 cataggttga tttgtctcga atgtcacgaa tttcatcaac accattattc gaggcagcat
     8461 caatttcaat cacatcttcc aagcttccat tcgtgatatc tcggcaaata tcgcattgat
     8521 tacagggttc accatcgact tggttaggac aattcatggc ctttgcaaaa atctttgccg
     8581 cacttgtttt cccagtccct ctaggacctg aaaaaagata agcatggcta atcttgccag
     8641 attcaactgc ctgctttaaa gttgtggaaa taaccgattg tcccaccatt tcgtcaaacg
     8701 tttggctccg gtatttccga taaagagctt gatacatcag tttttaactc caaacatgtt
     8761 aaaggtaaac gtggtctttt caagaaatag atccacaaag gcctctaagt attcttgatc
     8821 aacctcatca taatcagcta caagactaga atccaagtcc agcaccccaa tcaaatggcc
     8881 ttctttcacc ataggcacaa caatttcact catggctgcc gcatcacagg aaatatagtt
     8941 ggcatgctgc ttcacatcat ttataataat ggttcgacga gactgagctg attcgccaca
     9001 aacaccttta ccaagcttga tatggacgca agaaacccta ccttgaaaag ggccaagaat
     9061 taattcctgc ccatcaaaaa ggtaaaatcc cgtaaaaaca gaattgggta gtgtcatgtt
     9121 taacagggcg ctggcatttg acaaattagc caaagcattt gattcattcg caaacagctc
     9181 ttttgcctgc gctatcatta gttggtattg ctcaattttt ttagacttat tcataccttt
     9241 tattatagca tatctgggtc tattcgagta ttttttacga ctatctttcg cttcagtcag
     9301 tttttaaaat ggtttttgag gttgtttatg atat
//
LOCUS       DAXWIQ010000056         8641 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000056 DAXWIQ010000000
VERSION     DAXWIQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8641
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.056"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..1996
                     /locus_tag="ACGEUI_001530"
     CDS             <1..1996
                     /locus_tag="ACGEUI_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFF8166352.1"
                     /translation="LKSSVLSILTSFTLLVTDVQADQVDVQFLGVNDFHGALDNTGTA
                     YTPSGKIPNAGTAAQLGAYMDDAEIDFKQANQDGTSIRVQAGDMVGASPANSALLQDE
                     PTVKVFNKMKFEYGTLGNHEFDEGLDEFNRIMTGQAPDPESTINDITKQYEHEASHQT
                     IVIANVIDKKTKDIPYGWKPYAIKDIAINDKIVKIGFIGVVTTEIPNLVLKQNYEHYQ
                     FLDVAETIAKYAKELQEQHVHAIVVLAHVPATSKDGVVDHEMATVMEKVNQIYPEHSI
                     DIIFAGHNHQYTNGTIGKTRIVQALSQGKAYADVRGTLDTDTNDFIKTPSANVVAVAP
                     GIKTENSDIKAIINHANDIVKTVTERKIGTATNSSTISKTENIDKESPVGNLATTAQL
                     TIAKKTFPTVDFAMTNNGGIRSDLVVKNDRTITWGAAQAVQPFGNILQVIQMTGQHIY
                     DVLNQQYDENQTYFLQMSGLTYTYTDNDPKNSDTPFKIVKVYKDNGEEINLTTTYTVV
                     VNDFLYGGGDGFSAFKKAKLIGAINTDTEAFITYITNLEASGKTVNATIKGVKNYVTS
                     NLESSTKVNSAGKHSIISKVFRNRDGNTVSSEVISDLLTSTENTNNSLGKKETTTNKN
                     TISSSTLPITGDNYKMSPIMTILALISLGGLNAFIKKRKS"
     gene            complement(2078..2488)
                     /locus_tag="ACGEUI_001531"
     CDS             complement(2078..2488)
                     /locus_tag="ACGEUI_001531"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFF8166353.1"
                     /translation="MIREIITDHFLLQQKAQVAKKEDLWIGQDLQDTLAFYRQECLGM
                     AANMIGEQKRIVIVSMGFIDLVMFNPVMVSKKGIYQTKESCLSLSGYRKTQRYDKITV
                     EYLDHNWRPKRLSLTGLTAQICQHELDHLEGILI"
     gene            complement(2744..2962)
                     /locus_tag="ACGEUI_001532"
     CDS             complement(2744..2962)
                     /locus_tag="ACGEUI_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166354.1"
                     /translation="MALLFYYYVRKNQVKKLSQLKNLTEIFRKFIILTINNISAIKAF
                     QTAGVLFGPIKAANARESSIWFENLTKQ"
     gene            3205..3282
                     /locus_tag="ACGEUI_001533"
                     /pseudo
     CDS             3205..3282
                     /locus_tag="ACGEUI_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3380..5257)
                     /locus_tag="ACGEUI_001534"
     CDS             complement(3380..5257)
                     /locus_tag="ACGEUI_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8166355.1"
                     /translation="MSHFLVEKLTKTVGDKTVFQDISFIIHDFDRIGIIGVNGTGKTT
                     LLDVLSGRLGFDGDHSPFSKANDYKIAYLTQDPEFNDAASVLDTVLSADVKAIQLIRQ
                     YELLMANYTEDKQESLESLMSEMDRLDAWSIESDVKTVLSKLGITDLEQKVGDLSGGM
                     RRRVQLAQVLLGAADLLLLDEPTNHLDIDTIAWLTTYLKTAKKTVLFITHDRYFLDHV
                     ATRIFELDKAGLTEYQGNYQDYVRLKAEQDERDAANLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFSDLKKEVHQDSSADKLEMTFETSRIGKKVIHFEDLSFAYGDRQLIKD
                     FNLIIQNKDRIGIVGDNGVGKSTLLNIINGDLKPTSGKLDIGDTIRVGYFSQQLKDMD
                     ETKRVINYLQEVADEVKTSVGTTSISELLEQFLFPRSSHGTLIAKLSGGEKKRLYLLK
                     LLIEKPNVLLLDEPTNDLDIATLKVLENFLANFAGPVITVSHDRYFLDKVATKILAFE
                     EGDIRVFYGNYSDYLDEKVFEKETVEADLAKTTVTEEVPLPQKEERKRMSYLEKQEWA
                     QIEDKIATIEANIEEIENQMLTVVSDYGQLAQLQKELDQRNNDLLLAYERFEYLSGLD
                     I"
     gene            complement(5254..6462)
                     /locus_tag="ACGEUI_001535"
     CDS             complement(5254..6462)
                     /locus_tag="ACGEUI_001535"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFF8166356.1"
                     /translation="MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIH
                     DVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENGGEYEITTFRTEDIYVDYRRPSQ
                     VSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLKQKRLRAVGKAEERFEEDA
                     LRIMRGFRFAASLDFDIEAITFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGIS
                     AMIACQAYDYLPGLKQQEAGLNHLIVSLKDNFTFSDYHQAWAYVMISLAIEDPKSFLK
                     AWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKKMASLVEDLRKAQSLSVDMDR
                     INTLDQALVIHDKHDIVLNGSHLIKDFGMKSGPQLGLMLEKVELAIVEGRLDNDFTTI
                     EAFVREELAT"
     gene            complement(6587..7435)
                     /locus_tag="ACGEUI_001536"
     CDS             complement(6587..7435)
                     /locus_tag="ACGEUI_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8166357.1"
                     /translation="MKLAVITDSTATLPTDLKQDKAIFSLDIPVIIDDETYFEGRNLS
                     IDDFYQKMADSQNLPKTSQPSLSELDNLLGLLSSKGYTHVIGLFLAGGISGFWQNIQF
                     LAEEHPEIEMAFPDSKITSAPLGSMVKNVLDWSRQGMTFQAILNKLQEQIDGTTAFIM
                     VDDLNHLVKGGRLSNGSALLGNLLSIKPILRFDEEGKIVVYEKVRTEKKAMKRLVEIL
                     NDLIADGQYNVFIIHSKAQDKADYLKRLLQDSGYQYDIEEVHFGAVIATHLGEGAIAF
                     GVTPRL"
     gene            complement(7435..7815)
                     /locus_tag="ACGEUI_001537"
     CDS             complement(7435..7815)
                     /locus_tag="ACGEUI_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFF8166358.1"
                     /translation="MQLVREKEFVNQYHYDARNLEWEKENGTPETNFEVTFQLIDKDE
                     QQKETVIVSVLQFVIVKEEFVISGVISQMVRILDRLVDKPSEFTQEEVESLAAPLLDM
                     VKRLTYEVTEIALDRPGIHLEFKN"
     gene            complement(8043..8219)
                     /locus_tag="ACGEUI_001538"
     CDS             complement(8043..8219)
                     /locus_tag="ACGEUI_001538"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFF8166359.1"
                     /translation="MIKPYGFKYGLVGEVLRRIERLGFTFERLELRQASSKYLAKHDE
                     ALVINLFILDLKLT"
BASE COUNT         2727 a         1781 c         1369 g         2764 t
ORIGIN      
        1 tttaaaaagt agtgtattga gtatcctgac tagttttact ctattagtta cagatgttca
       61 agcagatcaa gttgatgtgc aattccttgg cgtcaatgat tttcacggcg ctcttgataa
      121 taccggaaca gcttacacac caagtggtaa aataccaaat gctgggacgg ctgctcaatt
      181 aggtgcttat atggatgacg ctgagataga cttcaagcaa gcaaatcaag acggaacaag
      241 tatacgtgtt caagctggag atatggtcgg agccagtcct gctaactctg cacttttaca
      301 agatgagcct actgtcaaag tctttaacaa aatgaaattt gaatatggca ctcttggtaa
      361 tcatgaattt gacgaaggac tagatgaatt taaccgtatc atgacaggtc aagcgcctga
      421 tcctgaatca acaattaatg atatcaccaa acaatatgag cacgaagctt cgcatcaaac
      481 catcgtcatt gctaatgtta ttgataaaaa aaccaaggat atcccctatg gttggaaacc
      541 ttatgctata aaagacatag ccattaatga caaaatcgtt aagattggct tcattggtgt
      601 tgtgactaca gagattccaa atctcgtttt aaagcaaaac tatgaacact atcaattttt
      661 agatgtagct gaaaccattg ccaaatatgc taaagaacta caagaacaac atgttcatgc
      721 tattgtggtt ttagctcatg ttcctgcaac aagtaaagat ggtgttgttg atcatgaaat
      781 ggctacggtt atggaaaaag tgaaccaaat ctatcccgaa catagcattg atattatttt
      841 tgcaggacat aatcatcaat acactaatgg aactatcggt aaaacacgta tcgttcaagc
      901 cctctctcaa ggaaaagctt atgcagatgt ccgtggtacg ctagatactg ataccaatga
      961 ttttattaaa actccatcag caaatgttgt tgctgtagca ccaggtatca aaacagaaaa
     1021 ttcagatatc aaagctataa taaatcatgc taatgatatt gttaaaacag ttactgaacg
     1081 aaaaatcgga actgcaacta attcttcaac tatttctaaa acagaaaata ttgataaaga
     1141 atctcctgtc ggtaacttag caacaacggc tcagcttact attgctaaga aaacttttcc
     1201 aactgttgac tttgctatga ccaataatgg tggtattcga agtgacctag ttgtcaaaaa
     1261 tgaccggacc atcacctggg gagctgcaca ggctgtacaa ccatttggta atatccttca
     1321 agtcattcaa atgactggtc aacacattta cgatgtccta aatcagcaat acgatgaaaa
     1381 ccagacctat tttcttcaaa tgtcaggttt aacatacact tatacagata atgatcctaa
     1441 gaactctgat acccccttca agatagttaa ggtttataaa gacaatggtg aagaaattaa
     1501 cttaacaact acttacaccg ttgttgtcaa cgactttctt tatggtggtg gtgatggctt
     1561 ttcagcattt aaaaaagcta aattaatcgg agctattaac acagatactg aagctttcat
     1621 cacatatatc acaaatttag aagcatcagg taaaactgtt aatgctacta taaaaggggt
     1681 taaaaattat gtaacttcaa accttgaaag ttcgacaaaa gttaatagtg ctggtaaaca
     1741 cagtatcatt agtaaggttt ttagaaatcg tgatggcaat acagtgtcta gtgaagtcat
     1801 ttcagacctt ttgacttcta ctgaaaacac taataacagc cttggcaaaa aagaaacaac
     1861 aacaaacaaa aatactatct ctagttccac tcttccaata acaggggaca attataaaat
     1921 gtctcctatt atgacaatcc ttgccttgat aagcttaggt ggactaaacg cttttattaa
     1981 aaaaaggaaa tcctagcgtt tactagtaat cgatcaccct tatataatca ggcatctttg
     2041 ccaaaaaaac ctaaagcgtc ctactctagg ttttttgtta aattaaaata ccttctagat
     2101 gatctaattc gtgctggcat atttgagcag ttaagcctgt taagcttaat cgctttggtc
     2161 gccaattatg gtccaaatac tcaacagtaa tcttatcata ccgctgtgtt ttgcgatagc
     2221 cagataacga caaacaagat tctttagttt ggtaaattcc ctttttagaa accatcacag
     2281 gattaaacat aaccaaatca ataaatccca tactcacaat aacaatgcgc ttttgttcac
     2341 caatcatatt ggcagccatc ccaagacatt cctgtctata aaaggctaag gtatcttgta
     2401 aatcttggcc aatccataga tcttctttct tggcgacttg cgctttttgc tgtaataaaa
     2461 aatgatctgt tatgatttct ctaatcattc ttatctcctt aaaccttctt tttaattcat
     2521 aaactacatg ttcaatggac tattatcact atatgataga cttagtatca tctgtttaaa
     2581 acatcaggtg attccttatt tcaagatatc ttgggcaact ttatgaaatt ctgcaatatg
     2641 agtaccaaat aaaaattgac ctgcatcagc agattgttaa taagttctca tcacaatatt
     2701 atcgaattta ctgtctgctt cttcaaatgt ctaagagaga tagttattgc tttgtgagat
     2761 tttcaaacca gatactgctt tctcttgcat ttgcagcctt gataggtcca aacaaaacac
     2821 ctgctgtttg aaatgcttta atagctgata tgttgttaat agttagtatt ataaattttc
     2881 tgaatatttc tgtcaagttt ttcagttgac ttaacttttt tacttgattt ttcctcacgt
     2941 aataatagaa aagaagcgcc ataatgtaaa ctacacccca aacgttagat aaaaaaatct
     3001 aacgtttggg gtgtttttgt atgacattaa gctatgaaga taagctagat atttagcgct
     3061 aaaaaaaccg agggaactcc gtttgaaaga agcatctctg caaagaagaa gacaaaaaac
     3121 cattataaag aatggacaaa gacgagttct gattaaattt tcttttaaga acggctaaat
     3181 tgactcgttc tacctactat tattatcgat tattgcaaca ataaccgtat taaaataaaa
     3241 cgaaaaggac ttagtcctct acaacacaga actaaatcct ttacttcaaa tagagtagcc
     3301 aacttttggg gctcagtgca agagcccttc ttttctatta tcacttgttt attataaaca
     3361 taacattacc atagtatact tatatgtcta gtccactcaa atactcaaac cgttcatacg
     3421 ccaataataa atcattattg cgctgatcga gttccttttg taattgagct aactggccat
     3481 aatcactaac tacagttaac atttggtttt caatttcttc aatattagcc tcaatagttg
     3541 ctattttatc ttctatctga gcccattctt gtttttcaag gtaggacatg cgctttcttt
     3601 cctctttttg aggaagtggc acttcttcag tgactgttgt tttagctaag tctgcctcaa
     3661 ctgtttcttt ttcaaaaact ttttcatcaa ggtaatcact gtaattgcca taaaaaacac
     3721 gaatatcgcc ttcttcaaag gctaaaatct tagtagctac tttgtctagg aaataacgat
     3781 cgtgactaac cgtaatgact ggtcctgcaa agttagctaa aaagttttct aagaccttca
     3841 aggtggcaat gtctaaatca tttgttggct catcaaggag aagaacattt ggcttttcaa
     3901 tgagcagttt tagcaagtag agccgttttt tctccccacc agataattta gcaatcagcg
     3961 tgccatgact tgatctcgga aaaagaaatt gttctaaaag ctcactgata ctcgttgttc
     4021 ccacacttgt tttaacctca tcagccactt cttgaaggta attaatgacc cgtttggtct
     4081 catccatatc tttgagctgc tgagagaaat aacctacacg aatagtatct ccaatatcaa
     4141 gcttcccaga agttggtttg agatcaccgt taatgatatt taacagggta gattttccga
     4201 cgccgttatc tccaacaatt ccaatacgat ctttattttg aataatcaag ttaaaatcct
     4261 taatcaactg tcggtcgcca taagcaaaag aaaggtcttc aaagtgaatc accttcttcc
     4321 caattcggct cgtttcgaaa gtcatctcca atttatccgc agaactgtct tggtgaactt
     4381 cttttttcaa gtcactgaaa cgattgattc tagcctgctg tttggttgca cgcgcctgtg
     4441 gttgcgtcct catccaggct aattcttgtt tatagagttg cttcttcttg tgtaaattag
     4501 cagcatctcg ttcgtcttgt tctgctttta agcgcacata gtcttggtaa ttgccttgat
     4561 attctgtcag tcccgcttta tccaattcaa aaatacgggt agccacatga tcaaggaaat
     4621 aacggtcatg ggtaataaaa aggacggttt ttttagctgt cttgagatag gttgttaacc
     4681 aagcgatggt gtcaatatcg agatggttgg tcggctcatc caacaagagt aagtcagcag
     4741 ctcctagcaa cacttgagct aactggacac gtcttctcat accacctgat aggtctccca
     4801 ctttttgttc caaatccgtg atgcctaatt ttgataggac tgttttgaca tcactttcaa
     4861 tagaccaagc atctaatcga tccatttcag acatgaggga ctctaagctt tcttgtttat
     4921 cctcagtata attcgccatc aatagttcgt actgacgaat cagttgaata gctttaacat
     4981 cagctgataa taccgtatct aaaacagaag ctgcatcgtt aaattcaggg tcttgtgtta
     5041 agtaagcgat tttgtaatca ttggctttag aaaatggtga atgatctccg tcaaaaccaa
     5101 gccttcccga caacacatct aaaagagttg tttttcctgt tccattaacc ccaataatcc
     5161 caatacggtc aaagtcatga ataataaagg aaatatcttg aaagacggtc ttatctccca
     5221 ccgtttttgt taatttctcc accaaaaaat ggctcatgtt gctaattcct ctctaacaaa
     5281 tgcttcaatt gtagtgaaat cattatctag gcgcccctct acaatcgcta attcaacttt
     5341 ctcaagcata agccctagct gaggacctga tttcatccca aaatccttaa tcaggtgaga
     5401 accattaagg acgatatcgt gtttgtcatg aataaccagt gcctgatcta aagtattaat
     5461 acgatccatg tcaacggata agctttgagc ttttcggagg tcttccacta gactcgccat
     5521 tttcttgccg tactggtaaa tatccaattt ttcaaaagat ctttcctgac gaattctata
     5581 aagagcaatc aactttgtga cataacgctg aaaatcattt gaagtcttcc atgcttttag
     5641 gaagctttta ggatcctcaa tggctaaact aatcatcaca taagcccaag cttggtgata
     5701 atcactaaag gtaaagttat ctttcaaaga aactatcaga tgattcaagc ctgcttcctg
     5761 ctgcttcaag cctggtaaat agtcgtaagc ctgacaagca atcatggcag aaatcccttt
     5821 tcgccagtgc ggtgccatca ataatttatc aaattcagta aaagaacgtt ccacagaaat
     5881 cttttccaat agaggtgaat ggctcctcat agcttcaaaa gttatagctt caatgtcaaa
     5941 atccagactc gccgcaaatc gaaagcctct cataatgcgt agtgcatctt cttcaaaacg
     6001 ttcctctgct ttacccactg ctcgtaaccg tttttgcttt aaatcaatga gtcctctaaa
     6061 tttatcaata acttgaccat tttcatccaa ggccaaggca ttgacggtaa agtctcttcg
     6121 ttttaagtct tcctctaaag agcgcacaaa agaaacctga ctaggacggc gataatcaac
     6181 atagatatct tctgtcctaa aggttgtaat ttcatactca ccgccatttt caagcacgag
     6241 cactgtacca tgttcaatgc caacatcgac agttctgtta aaaatcgctt tagtctcttc
     6301 aggataggaa ctagttgcaa tatctacatc atgaatggga cgctcgagta aaacatctct
     6361 gacgcttcct cccacaaaat aggcttcata accagcttct tttattttag tcaatatggg
     6421 taaagccttc tgaaactcag aaggcattgt cattaatttc atacgttgtt ttctaaccta
     6481 tcatccaact agcccaatct aaagtgtgat taggctaatt gggttctatc acattttgtt
     6541 attgttttag tgattcttgc cattaactat tttttctaat cgacaattaa agcctcggtg
     6601 ttacgccaaa tgcaatggct ccttctccta aatgagtagc aatcaccgct ccaaaatgaa
     6661 cctcttcaat gtcatattga taaccactgt cttgtaagag tcgtttcaaa taatcagctt
     6721 tgtcttgtgc ttttgagtga ataataaaca cattatactg accgtctgct atcaagtcat
     6781 ttaaaatctc aaccaagcgt ttcatggctt tcttttctgt tctcactttt tcataaacaa
     6841 cgatttttcc ctcttcatca aagcgtaaaa taggtttaat actcaaaaga tttccaagaa
     6901 gagcagaccc atttgacagg cgtccgcctt taaccagatg attaaggtca tcaaccataa
     6961 taaaggctgt cgtcccatca atctgttctt gcagcttgtt taaaatagct tgaaatgtca
     7021 ttccttgacg tgaccaatct agcacatttt ttaccatact acctaaagga gcagatgtga
     7081 tcttactgtc tggaaatgcc atttcaatct caggatgttc ttcagctaaa aattgaatat
     7141 tttgccaaaa gcctgagatt cctccagcta agaacaagcc aataacatgt gtataacctt
     7201 tagaagataa aagtcctaaa agattatcta gttcagataa acttggctga cttgtcttgg
     7261 gaagattttg ggagtcagcc atcttttgat aaaaatcatc tatactcaaa tttcgacctt
     7321 caaaataggt ctcatcgtca atgataactg gtatatcaag gctaaaaata gctttatctt
     7381 gtttcagatc tgttggtagt gttgctgtac tgtcagtaat tactgctaat ttcattaatt
     7441 tttaaactcc aaatgaatac ctggacgatc caaggcaatt tctgtgactt cataagtcaa
     7501 tcgcttaacc atatctagca aaggagcagc taaggattca acttcctctt gcgtaaattc
     7561 gcttggttta tccactaaac gatctaaaat acgaaccatt tgtgagatga ccccactaat
     7621 aacaaattct tctttgacaa taacaaattg caatacagat acaattactg tttctttttg
     7681 ctgctcatct ttatcaatca actgaaaagt cacttcaaaa ttggtctctg gtgttccgtt
     7741 ttctttttcc cactctaaat ttctagcatc ataatggtac tggttgacaa attctttttc
     7801 acgaactaat tgcatttttt attctccttg gtatcaatat gatccattat agcatagatt
     7861 tgaaaattat taaagtagcc cctaaactac tattttcgca taacgtttta aacagtgcta
     7921 tctctcgaac agatatctat tttttcacct atttatattt agtcgtcctt atactgacat
     7981 tataccaaaa aaacacccgt tagtggtctt gtactgcacc tcaattaaaa cagaaccact
     8041 ggtcaggtaa gttttaagtc caggataaaa aggtttatca ccaaggcctc atcatgctta
     8101 gccaagtatt tcgaactagc ttgtcttagc tctaatcgct caaacgtaaa ccctaggcgt
     8161 tcaatccgcc gcaaaacctc tccaactaac ccatacttaa acccataggg cttaatcatc
     8221 gctccgccct tcttctttta tccattataa aataaaaacg ttttaacaga caaaaaatag
     8281 gaaagtacca gactattgga tttatttttt tatctcaaac tccattaacg atgttgagtt
     8341 aaaggcattc aaaaccacat agatcgtaag ctaacaagat tttttatcaa ttgaacctat
     8401 cgtccccagt tatacacgac ctcttctgga tgaggtcttt ttttatttgt ctaatttcat
     8461 caacgcttct tcccctcttt cttctatcac agctctccta ttgctcactt cctcatgcag
     8521 caacatatca tcaaaaaacc gttcattttt aagcacaacc actactgtat ctcttgtact
     8581 gtctccattg caatcgcatg tttaatttta ttaaaaatta tttaaaaaaa ttaaatatta
     8641 t
//
LOCUS       DAXWIQ010000057         8626 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000057 DAXWIQ010000000
VERSION     DAXWIQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.057"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            21..236
                     /locus_tag="ACGEUI_001539"
     CDS             21..236
                     /locus_tag="ACGEUI_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8166360.1"
                     /translation="MSQQHKKWSALVEQRLKEKGWTRVDLALAAGLRSSGTITDLLTK
                     GKGSVDLKVRVSKILSIREPWELFENE"
     gene            298..573
                     /locus_tag="ACGEUI_001540"
     CDS             298..573
                     /locus_tag="ACGEUI_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFF8166361.1"
                     /translation="MDSRLLQMLDEFEAGLIDRKIKVMKMINNETEIYPLELNKKQVS
                     KMFGVDPKTFDARFNSHKDFPRIETGGREKYPRDLAIEWYHRNWSIT"
     gene            570..740
                     /locus_tag="ACGEUI_001541"
     CDS             570..740
                     /locus_tag="ACGEUI_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166362.1"
                     /translation="MNKLELFLLATTVILAVIARVQHEVIKKHNSPENKRRIFREVAL
                     ENSKKWSAERYV"
     gene            733..936
                     /locus_tag="ACGEUI_001542"
     CDS             733..936
                     /locus_tag="ACGEUI_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166363.1"
                     /translation="MSKWELQKQLLANATDFESTVEALFELNELGEISDEELLLNLRS
                     SYRKYSEEKLCIIDKLIKLGGAE"
     gene            933..1319
                     /locus_tag="ACGEUI_001543"
     CDS             933..1319
                     /locus_tag="ACGEUI_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166364.1"
                     /translation="MMTTFEKIIKNLNRIERELVKPEAERRDSLPILSAQLSAIKQDL
                     KLMLWIELPALNNVEKYEALYQGNDFKFSETFLERQATRKAFWRRLAKETFKEDQDKR
                     NFVKLAEKEFKGATLSWQEFLWGKKK"
     gene            1465..1668
                     /locus_tag="ACGEUI_001544"
     CDS             1465..1668
                     /locus_tag="ACGEUI_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166365.1"
                     /translation="MNYLEYALAYLERELEIIDDEVIEVELPGGDWEFVPNPYYEEGL
                     HDSPHYRSQVAKDILDIKGLLGR"
     gene            1756..2055
                     /locus_tag="ACGEUI_001545"
     CDS             1756..2055
                     /locus_tag="ACGEUI_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166366.1"
                     /translation="MIEVTFKAESMAGVFDAMREALDMPKTVTKDVVEEKPAPKKKEE
                     SVTLTLADIKKMAKAKVEEGKSKDIKEVLKEMNVAKVGELEEYQFAEFVEKLEAL"
     gene            2055..3209
                     /locus_tag="ACGEUI_001546"
     CDS             2055..3209
                     /locus_tag="ACGEUI_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFF8166367.1"
                     /translation="MPVENHALLSASSAHRWLYCPMLPRLEADYPSRDTVYTQEGTSA
                     HELSEIKLMYKSGKITKRKFNTLTKAFKENSDFYNEEMEEMTELYTDIVMEHLNAYED
                     AEIELEKRIDFSDWVPGGFGTSDVVILADGVIEIIDLKYGKGMPVSANQNPQMGLYAL
                     GAYASYDMVYDFDRIKMTIIQPRLDSVSSVDIYVEELLYWADNVVLPMAAQADAGIGD
                     WNLSEKVLQWSPVAAKLVPRAQENWELIDKYDYQEPVYLSDEAVAEILDKASAIKKWV
                     ESVEAYALKEALSGKEVPGYKIVEGRSNRVLTNKEAAATILEKNGFEDIYKPKELLSM
                     GALEKMVGKKRFEDMMAFILDKPQGKPVLVKNSDKRPALNSLEQAIKEFE"
     gene            3213..3611
                     /locus_tag="ACGEUI_001547"
     CDS             3213..3611
                     /locus_tag="ACGEUI_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166368.1"
                     /translation="MKIYKVKVKFRQTCHKKFKGKKYSYFSFEELRVGDLVVVETVYG
                     PSVAKVTEVVDANELFTATSYVISKVDTSLLAGKKELMATALTVKANIDAETAEFAAK
                     YKDAYYLGLFDQYKNQNPELAELLTQLKEL"
     gene            3622..4179
                     /locus_tag="ACGEUI_001548"
     CDS             3622..4179
                     /locus_tag="ACGEUI_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFF8166369.1"
                     /translation="MTPSTTKVVTGKVRLSYAALLEPKAFEGQEAKYSTVILIPKTDK
                     VTIKKIKDAQKAAYEAAKDNKLKGVKWERVKTTLRDGDEEMDTEEHPEYAGHMFMSVS
                     SKTRPQIIDRYKNPVDSADEVYSGVYARVSLNAYAYNTAGNKGISCGLNNVQIVAKGD
                     YLGGRSSADADFDEWNEEEDEDDIL"
     gene            4222..6144
                     /locus_tag="ACGEUI_001549"
     CDS             4222..6144
                     /locus_tag="ACGEUI_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFF8166370.1"
                     /translation="MRHLNIDIETYSSNDIKNGVYKYADAEDFEILLFAYSIDGGEVE
                     CLDLTRQSLPEDIKDMLFDDKVRKHAFNAQFERVCLSRYLGLPYYLDPAQWQCTMVLA
                     QELGLPASLEKCALYLKLAQEKDAAGKNLIKYFSVPCKPTKANGGRTRNLPEHDPGKW
                     QMFINYCMQDVVVEMAIAEKLESVPVHDREWDYYVCDQRINDRGVALDKELVASALYC
                     KDVKMESLSGELKALTGLDNPNSRAQLLPWLKEHGYSANGLTKADVEQELKKAEGELK
                     RVLELKLQTAMSSLKKYEAMERAMCSDGRVHGLLQFYGASRTGRWAGRVVQVQNLARN
                     YIKDLDDAREYVKKRDIDAVEILYDSLNDTLKQLVRTALVAKDGYTFYVSDFSAIEAR
                     VIAWFAGEQWRLDVFSTHGKIYEASASQMFGIPIEEIDKELRQKGKISELALGYQGGP
                     GALKQMGALNMGVKEEELQGLVDDWRRANKKIVQFWKDVQRAAIKAIKSRAPIKLGKI
                     RFRYRKGFLFITLPSGRNLAYARAKVEPGDYGDKIIYEGQGDRAYFTAQETYGGKLVE
                     NIVQATARDILAEALLRIEAAGYDVVFHVHDEAIIEGSGLIIEEVNDLMAQAPEWAEG
                     LPLNSEGYVTKYYMKD"
     gene            6149..8533
                     /locus_tag="ACGEUI_001550"
     CDS             6149..8533
                     /locus_tag="ACGEUI_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFF8166371.1"
                     /translation="MKQEKLIVKSSPLQELHIATGSSRTAKTWKNITLTWQELVERLE
                     KPTVTQETFAEYQKMSRAEKGQAKDVGGFVGGWLKQGKRKNENVQSRSLVALDADSPS
                     KDFLDRLDLLADYAFVLYSTHSHSKKAAKYRLIIPTDRLMMPDEYEPVARYLANQLGM
                     SNFDDTTYQSVRLMFWPSHSRDADFTFKYNDEAFLSVDEVLDTYPDWHDSSFWPESPT
                     HAVKRQREAKKQGDPLSKKGLIGAFCRNYDIRQAIATFLSEVYEEGTTPDRYTYTDGS
                     TANGLVIYDDVFAYSHHGTDPVGDTLVNAYDLVRIHKFGEQDSEAKDNTPTNKLPSSK
                     AMNAFVSDLPEIKDHLMAEALGDFDEELPVEDDRSWLEIDERGEPEVNSYLLATQIIK
                     EVPIYWDGLEFLRYDAKKGIWLPNAEEYLKSYISTKKLGKITKIRHISETVVAIKAQA
                     FSSEVFTESDLNKIVLANGVYDLRDNNFKTKFDPELHARSSHPVVYDPEATCETFEGF
                     LRETVGAENIDFIFEWFGYNFYREYTIQKMLFIYGSGGTGKSTLINILREMIGADNYS
                     AVTLQYLMQERFAKIGLYRKTANFDTDAKPQYLADGAALKMLTGEDTIHADRKNKEPI
                     NFYNYAKLSFAMNELPPMRDFSGGLKRRMMILEMDKVLTQEVKAKYPLDKIMSEVPGI
                     FNRAMEGLRKALSKRDFSISASMRSSVEKWEKGNDVVAMFLEDECELGEDFKVPVRDV
                     YPAYKFYCQDSGYKPLAKNAFNHRLRELNYENKNVKSDGKQAKNWVGFRLKSEF"
BASE COUNT         2802 a         1529 c         2094 g         2201 t
ORIGIN      
        1 tatatcagaa aggagagcag atgagtcaac aacacaaaaa atggtcagct ctagttgaac
       61 agcgattaaa agaaaaaggt tggacaagag tcgacttggc acttgcggct ggtttgcgga
      121 gttcaggaac gattactgac ttacttacta aaggaaaagg aagcgttgac cttaaggtta
      181 gggtttcaaa aattcttagt atccgtgagc cgtgggagtt gttcgaaaat gaatagtaca
      241 caaaaaagcc actgctggaa cagtgactta gctaaaacat attaaaggaa ttttatcatg
      301 gatagcagat tattacaaat gctagatgag tttgaagcgg gccttattga tcgcaaaatc
      361 aaagtcatga aaatgattaa caatgagacg gaaatttatc cgctagaact caataaaaag
      421 caagtcagta agatgtttgg ggttgaccct aaaacatttg acgcaagatt taacagccac
      481 aaagatttcc cgcgtattga aacggggggt cgggaaaaat acccacgaga tttagccatc
      541 gagtggtatc acagaaattg gagtataaca tgaacaaatt agaactattt ttattagcaa
      601 caaccgtcat cttagcggtc attgctaggg tgcaacacga agtcatcaaa aaacacaatt
      661 cgccagagaa taaacgcaga atttttaggg aagtggcttt agaaaacagc aagaaatgga
      721 gtgcagaacg ttatgtctaa atgggaatta caaaagcaac tgttggctaa tgcgacagat
      781 tttgagagta cagtagaagc tctgtttgag ttaaatgaat tgggtgaaat ctcggacgag
      841 gagctcttgt taaatttgag aagtagctac aggaaatact cagaggaaaa actttgtatt
      901 attgacaaac tcattaagct tggaggcgca gaatgatgac aacttttgaa aagattatca
      961 agaaccttaa tcggattgag agggaattag ttaagcccga agctgaaaga cgagatagct
     1021 tacctatcct atctgctcag ttgagtgcta tcaagcaaga tttaaaattg atgctttgga
     1081 ttgaacttcc ggcgttaaat aacgtagaaa aatatgaagc tctttatcaa ggaaatgatt
     1141 ttaaattcag tgaaactttc ttagagcgtc aagcgacgag aaaagccttt tggagacgat
     1201 tagctaaaga aactttcaaa gaagaccagg ataagaggaa ctttgttaag ttggctgaga
     1261 aagaatttaa aggtgcaact ttgtcatggc aagaattttt gtggggtaaa aagaaatgaa
     1321 catatcacta aaatttattg atgaaaccct agctggttta aatgatattt tacgccaggg
     1381 ggggctctct tgttcccaaa gccaagccct ggcggacgca gtttttatat taacagcttt
     1441 aaaacaagta attgaggaga gaaaatgaac tatttagaat atgcactagc ttatcttgaa
     1501 cgtgagctag aaattattga cgacgaagtt atcgaagttg agttgccggg cggagattgg
     1561 gagtttgtgc ctaaccccta ctacgaggaa ggtttacatg atagcccaca ctatcgtagt
     1621 caggttgcta aagacattct tgatattaaa ggacttttgg ggaggtgagg ctattgattg
     1681 catcaaaaaa gacggcttag gaaaccgtca acaattatac acaattaaat tataacacag
     1741 aaagagagaa acacaatgat tgaagtaact tttaaagcag agagcatggc aggtgttttc
     1801 gatgctatgc gtgaagctct ggatatgccg aagacagtaa ccaaggatgt tgttgaagaa
     1861 aaaccagctc ctaaaaagaa agaagagtct gtaactttga cattagctga tattaagaaa
     1921 atggctaaag ccaaggttga agaagggaag tcaaaggaca tcaaggaagt cttgaaagag
     1981 atgaacgtcg caaaagttgg tgaacttgaa gaataccagt ttgctgagtt tgttgagaag
     2041 ttggaggccc tctaatgcca gtagaaaatc acgcactact gtctgcttct agtgctcaca
     2101 gatggcttta ttgccctatg ctgccgagat tagaagcaga ctaccctagc cgcgataccg
     2161 tatacactca agagggcaca agcgcccatg agctttctga aatcaaactg atgtacaagt
     2221 ctggtaaaat caccaagcgt aaatttaaca cgttaaccaa ggcttttaag gaaaactcag
     2281 acttctacaa cgaagagatg gaagagatga cggaacttta cacagatatt gttatggagc
     2341 acctaaatgc ttatgaggat gccgaaattg aacttgaaaa acggattgat tttagcgact
     2401 gggtgcctgg cggttttggg acttcggatg tcgtcatttt ggcggatggg gtcattgaaa
     2461 ttattgatct taagtatggc aagggtatgc ctgtgtctgc taaccaaaac ccgcagatgg
     2521 ggttgtacgc tctgggagct tacgcttcct acgatatggt ttatgacttt gaccgtatca
     2581 agatgacaat tattcagccg cgtttagatt cggttagttc tgttgacatt tacgtagagg
     2641 agcttctgta ttgggcggat aatgttgtct tgcctatggc cgctcaagca gacgcaggta
     2701 ttggcgactg gaacctaagt gaaaaagtat tgcagtggtc tcctgtcgca gctaaattgg
     2761 tgccaagagc gcaagaaaac tgggaattaa ttgataaata tgactatcag gagcctgtct
     2821 atttatctga tgaagccgtc gcagagatcc ttgacaaagc ctcagctatc aagaagtggg
     2881 ttgagtcagt tgaagcctat gccctgaaag aagcactctc aggaaaagaa gttccaggct
     2941 ataagattgt cgaaggtaga agtaatcgtg tgcttactaa taaggaggca gctgcgacaa
     3001 tacttgagaa aaacggcttt gaagatatct acaaaccaaa agagctactg tccatggggg
     3061 ctctagagaa gatggtcggt aagaagcgtt ttgaggacat gatggccttt atccttgata
     3121 aaccacaagg taaacctgtc cttgtcaaaa atagcgacaa gcgacccgcg ttaaatagct
     3181 tagaacaagc aataaaggag tttgaataga atatgaagat ttataaagta aaagttaagt
     3241 ttagacaaac atgccataaa aaattcaagg gtaagaaata cagctacttt agttttgaag
     3301 agttgcgggt aggtgacctt gtggttgtcg aaactgttta tggccctagc gtagccaagg
     3361 taacagaagt tgttgatgcc aatgagcttt ttacagctac cagctatgtt atttccaaag
     3421 tggatacttc acttttagcg ggcaagaaag agttaatggc taccgccttg actgtaaaag
     3481 caaatattga tgcggaaacg gctgagtttg cagcgaaata taaagatgct tattatttag
     3541 gcctgtttga tcagtacaaa aaccaaaatc cagaattagc agagttatta acacaattaa
     3601 aagaattata ggagaagaat tatgacacca agcacaacta aagtagtaac cggaaaagta
     3661 cgcctaagct atgcagcttt actagagcct aaagcctttg aaggtcaaga ggctaagtat
     3721 tcaacagtta tcttaattcc aaaaacagac aaagtcacaa tcaagaaaat taaagacgcg
     3781 cagaaagctg cttatgaagc tgccaaggac aataaactca aaggggttaa atgggagcgc
     3841 gttaagacaa cgcttcgtga cggtgacgaa gagatggata ctgaagagca cccagagtac
     3901 gctggacaca tgttcatgtc agtgtcaagc aaaactaggc cgcagattat cgaccgttac
     3961 aaaaaccccg ttgactccgc agacgaagtt tactcaggag tatacgcacg agtatcatta
     4021 aatgcctatg cctacaatac tgcgggtaac aagggtatct cttgtggtct gaataacgtt
     4081 cagattgttg ctaaaggaga ctaccttggc ggtcgttcgt cagctgatgc agactttgac
     4141 gagtggaacg aagaagagga cgaagacgac attttatagt agagggcctc tttagaggct
     4201 cctcattttt aaaggagagg tatgagacat ttaaatattg atattgaaac ctatagttcg
     4261 aatgacatca aaaatggggt ttacaagtac gctgacgctg aagattttga gattttactt
     4321 ttcgcttact ctatagatgg cggagaagta gagtgccttg atttgacaag gcagtctcta
     4381 cctgaagaca tcaaagatat gttatttgat gataaagtcc gaaagcacgc ctttaatgcc
     4441 caattcgaaa gagtttgtct cagtcgttac ctcggcctac cttactattt agatcccgca
     4501 caatggcagt gtacgatggt acttgcacaa gagctgggtc tgcctgcaag cctggaaaaa
     4561 tgtgcgctgt atttaaaatt agcgcaagaa aaagacgcag caggtaaaaa tctcattaaa
     4621 tatttttcgg taccctgcaa gcctacaaaa gccaacgggg gaagaacgag gaacctgccc
     4681 gagcatgatc ctggaaaatg gcaaatgttt attaactact gtatgcaaga cgttgttgtt
     4741 gagatggcaa ttgccgaaaa actagagtca gttcctgtgc acgaccgtga gtgggattac
     4801 tacgtctgcg accagagaat caacgacaga ggcgtggcac ttgataaaga gttagttgct
     4861 tcggctttgt attgtaaaga tgttaagatg gaaagtttgt ctggtgaact aaaggctcta
     4921 acaggacttg ataatcctaa tagcagggcg cagctgctac cgtggctaaa agaacacggt
     4981 tattcggcta atgggctgac taaagctgat gttgaacagg aacttaagaa ggccgaagga
     5041 gagcttaaga gagttttaga acttaaacta caaaccgcga tgtcaagtct aaaaaaatat
     5101 gaagctatgg aaagagctat gtgctcagac ggacgagttc atgggctact tcagttttac
     5161 ggggctagcc ggacaggaag atgggcgggc agagttgtcc aagtacagaa cttagctagg
     5221 aattatataa aggatctaga tgatgctaga gagtatgtta aaaagcgtga tattgatgct
     5281 gtggagattt tatacgatag ccttaacgac actttaaagc agctcgtgcg aacggcactc
     5341 gtggctaaag atggctatac cttctatgtc tctgacttct cggcgattga ggctagggtg
     5401 attgcatggt ttgctggaga gcagtggagg cttgacgtgt tttctacgca cgggaaaatc
     5461 tacgaggcgt ctgccagtca gatgttcgga attccaattg aggagattga caaggaacta
     5521 cgccaaaagg gcaaaatttc agagttagca cttggctatc aaggaggtcc tggagcgctt
     5581 aagcagatgg gggctctaaa tatgggagtc aaggaagagg agcttcaagg gttagttgat
     5641 gactggcgca gggccaataa gaaaatcgtc caattttgga aagacgtaca aagagccgcc
     5701 atcaaagcca tcaaatcgag agcaccgata aaacttggaa aaatacgatt tagataccgg
     5761 aaaggtttcc tctttataac attgcctagc ggtaggaact tagcttatgc aagagccaag
     5821 gttgagccag gcgactatgg agacaaaatc atctatgagg gccaaggaga tagggcctat
     5881 ttcacagcgc aagagacgta cggcggtaag cttgtcgaaa atatcgttca ggcgacggcc
     5941 agggatattc tagctgaagc gcttctgagg attgaagctg caggctatga tgttgttttc
     6001 cacgttcatg atgaggctat tatcgaaggt tcaggcctga taatcgaaga ggttaatgat
     6061 ttgatggctc aggctcctga atgggcggag ggtcttcctt taaatagcga aggctatgta
     6121 acaaagtatt atatgaagga ttagatagat gaagcaagaa aaactaatag taaagtcttc
     6181 tcccctgcaa gagcttcata tcgcaacagg tagttcgcga acagctaaga catggaaaaa
     6241 tatcacgcta acttggcagg agctggttga gaggttagag aaacctacag tcacccaaga
     6301 gacgtttgcg gagtaccaga agatgtctcg agcagaaaaa gggcaagcaa aagacgtagg
     6361 gggctttgtc ggtggatggc taaagcaagg taaacggaaa aatgaaaacg ttcaaagtag
     6421 gtccttggtt gcgcttgacg cagatagccc aagtaaagat ttcttagata ggctagacct
     6481 gcttgcagat tatgcattcg tactctacag tactcacagc cactcaaaaa aagctgctaa
     6541 gtaccgtctt attatcccta ctgaccgttt aatgatgcct gatgaatatg agccagtcgc
     6601 tagatatttg gctaaccaac taggcatgtc aaactttgat gacacgactt atcaaagcgt
     6661 acgcttgatg ttctggccga gccactcaag agatgccgac tttacgttta aatataacga
     6721 cgaggctttt ctgagtgttg atgaggtgct tgacacatac ccggactggc atgactcaag
     6781 cttctggcca gaaagcccga cgcacgctgt taaaagacag cgtgaagcta aaaaacaagg
     6841 tgacccgctt agtaaaaaag gacttattgg agccttttgt cgtaactatg acattagaca
     6901 ggccattgca acattcttat ctgaggttta tgaagaagga acgactcctg ataggtacac
     6961 ctacactgat ggctcaaccg caaacggctt agttatctat gatgatgtct tcgcttatag
     7021 ccatcacggg acagatcccg tgggggatac gcttgtaaat gcctatgacc ttgttcgtat
     7081 ccataaattc ggagagcaag atagcgaggc taaagataat actcctacta ataagttgcc
     7141 gtcaagtaaa gcgatgaatg cttttgtctc tgacttaccc gaaattaaag accatttaat
     7201 ggcggaggct ttaggcgatt tcgatgaaga gttaccagtc gaagatgaca gaagctggct
     7261 tgaaattgat gagaggggcg aaccggaggt caatagttat ttgctagcaa cgcagattat
     7321 taaggaggtt ccgatttatt gggatggctt agaattttta cgctacgacg ctaaaaaagg
     7381 catctggttg ccaaacgctg aggaatattt gaagagttat atctcaacta agaaactcgg
     7441 taaaattact aagattaggc acattagcga aaccgtcgta gcgattaaag cacaggcttt
     7501 ttcaagtgaa gtgtttaccg agagtgatct taacaagata gtgctagcga atggagtcta
     7561 tgacctgagg gataacaact ttaagactaa gtttgatcca gaattgcatg ccaggtcaag
     7621 ccatcccgtt gtctatgacc ccgaggcgac ctgtgaaacc tttgagggtt ttcttagaga
     7681 gaccgtcgga gctgaaaata tagatttcat ctttgagtgg tttggctata acttttatcg
     7741 tgaatacact attcaaaaaa tgctattcat ctatggtagc gggggcactg gcaaatcaac
     7801 actaattaat attttacgtg aaatgatagg cgctgataat tattcagccg tgacactgca
     7861 gtacctgatg caagaacgct ttgcaaaaat cggcttatat cgtaagacag ctaacttcga
     7921 taccgatgct aaacctcaat acttagcaga tggcgcagca cttaaaatgt tgacggggga
     7981 ggatactatt cacgcagacc gtaagaataa agagccgatt aacttttaca attatgctaa
     8041 gctgtctttt gccatgaatg agctcccacc tatgcgagat ttcagcggag gacttaaacg
     8101 ccgcatgatg atcctcgaga tggataaggt tttaacgcag gaagttaagg cgaaataccc
     8161 gctagataag attatgagcg aggtgcctgg tatctttaac agagcgatgg aggggcttag
     8221 aaaggcctta agtaagagag atttcagtat tagtgccagc atgaggtcaa gtgtcgagaa
     8281 atgggaaaaa ggcaacgatg tcgtagctat gttccttgaa gacgagtgtg aacttggcga
     8341 agacttcaaa gttcctgtta gggatgtcta cccagcttat aaattctatt gtcaggattc
     8401 aggctacaaa cctttggcaa aaaatgcatt taaccatcgg ctaagagagc taaattatga
     8461 aaataaaaac gttaaatctg atggaaagca agccaaaaat tgggtcggct ttaggttaaa
     8521 aagtgagttt taggggttac ttttttggaa ttagagtttg gtaaccctag aaaaaagtta
     8581 ccaagttacc ggttttttca aactttaaaa ataaaaaatt taaaaa
//
LOCUS       DAXWIQ010000058         8618 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000058 DAXWIQ010000000
VERSION     DAXWIQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8618
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.058"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(432..1838)
                     /gene="lacG"
                     /locus_tag="ACGEUI_001551"
     CDS             complement(432..1838)
                     /gene="lacG"
                     /locus_tag="ACGEUI_001551"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFF8166372.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLSEEFGVNGIRISIAWSRIFPTGKGEVNPKGVEYYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSDGDFLNRENIEHFVNYAEFCFKEFSEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLFKDSGYSGEIGVVHAL
                     PTKYPFDANNPDDVRAAELEDIIHNKFILDATYLGKYSDKTMEGVNHILEVNGGELDL
                     REEDFAALDAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     KAPVDVPKTDWDWIIFPQGLYDQIMRVKADYPNYKKIYITENGLGYKDEFVDNTVYDG
                     GRIDYVKKHLEVISDAISDGANVKGYFMWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            complement(1926..3623)
                     /locus_tag="ACGEUI_001552"
     CDS             complement(1926..3623)
                     /locus_tag="ACGEUI_001552"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFF8166373.1"
                     /translation="MNGLIAQIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIENLLMTPYNYSMGILGLFVAGTTAKALTDSMNRSLPSTNQ
                     INFISTMLAAIVGFLLMAANPAKDGGFLTGFMGTKGLLTAFIAAFITVNVYKACVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTLSVVLLYVIELIVHTTLGVNVAESIGKLLAPLFQ
                     AADGYVGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAETNLQLLQAGEHADKIL
                     TSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPL
                     VLNPIFFIPFILAPIVNVWIFKFFIDTLGMNSFTANLPWTTPAPLGLILGTNFQVLAF
                     ILAALLIVVDVIIYYPFLKVYDEQILAEEAAGTNSSDALKEKVAANFDTKKADAILEK
                     SAAKETKEITDQTNVLVLCAGGGTSGLLANALNKAAKEYGAPVTAAAGSYGAHREILP
                     QYQLVILAPQVASNYEDMKVETDKLDIKLAKTEGAQYIKLTRDGQGALDFVKAQFEN"
     gene            complement(3623..3940)
                     /locus_tag="ACGEUI_001553"
     CDS             complement(3623..3940)
                     /locus_tag="ACGEUI_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8166374.1"
                     /translation="MNREETTLLGFEIVAYAGDARSKFLEALKAAQEGDYAKAEELIA
                     AGSDCLNDAHNAQTSLLQKEAAGDDLAYSVTLMHGQDHLMTTILLQDLMKHMIELYKR
                     GAK"
     gene            complement(3964..4947)
                     /gene="lacD"
                     /locus_tag="ACGEUI_001554"
     CDS             complement(3964..4947)
                     /gene="lacD"
                     /locus_tag="ACGEUI_001554"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8166375.1"
                     /translation="MTITLTENKRKSMEKLSVDGVISALAFDQRGALKRMMAQHQTKE
                     PTVEQIEELKSLVSEELTPFASSILLDPEYGLPASRVRSEEAGLLLAYEKTGYDATTT
                     SRLPDCLDVWSAKRIKEAGAEAVKFLLYYDIDGDQDVNEQKKAYIERIGSECRAEDIP
                     FYLEILTYDEKIADNASPEFAKVKAHKVNEAMKVFSKERFGVDVLKVEVPVNMKFVEG
                     FADGEVLFTKEEAAQAFRDQEASTDLPYIYLSAGVSAKLFQDTLVFAAESGAKFNGVL
                     CGRATWAGSVKVYIEEGPQAAREWLRTEGFKNIDELNKVLDKTASPWTEKM"
     gene            complement(4951..5880)
                     /locus_tag="ACGEUI_001555"
     CDS             complement(4951..5880)
                     /locus_tag="ACGEUI_001555"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFF8166376.1"
                     /translation="MILTVTLNPAIDVSYPLDELKCDTVNRVVDVTKTPGGKGLNVSR
                     VLNEFGETVKATGCVGGESGDFIINHLPDSILSRFYKISGDTRTCIAILHEGNQTEIL
                     EKGPMLSVDEIDGFTHHFKYLLNDVDVVTLSGSLPAGMPDDYYQKLIKIANLNGKKTV
                     LDCSGNALEAVLKGDSKPTVIKPNLEELSQLLGKEMTKDFDALKEVLQDELFDGIEWI
                     IVSLGADGVFAKHKDTFYNVDIPKIKIVSAVGSGDSTVAGIASGLANDEDDRALLTKA
                     NVLGMLNAQEKTTGHVNMANYDKLYQSIKVKEV"
     gene            complement(5928..6443)
                     /gene="lacB"
                     /locus_tag="ACGEUI_001556"
     CDS             complement(5928..6443)
                     /gene="lacB"
                     /locus_tag="ACGEUI_001556"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFF8166377.1"
                     /translation="MKIAVGCDHIVTYEKIAVVDYLKSQGHKVIDCGTYDNVRTHYPI
                     FGKKVGEAVASGEAELGVVICGTGVGITNAVNKVPGIRSALVRDMTSAIYSKEELNAN
                     VIGFGGKIIGGLLMNDIIDAFLAAEYKPTEENKKWIEKMDSLQHASQDQNNPHFFDEF
                     LEKWDRGEYHD"
     gene            complement(6478..6906)
                     /gene="lacA"
                     /locus_tag="ACGEUI_001557"
     CDS             complement(6478..6906)
                     /gene="lacA"
                     /locus_tag="ACGEUI_001557"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFF8166378.1"
                     /translation="MAIIIGADKAGQELKEVIKDYLKEGKYEVVDVSENEVRDFVDTT
                     LAVVKEVNASDDNLGIVIDAYGVGSFMVATKIKGMVAAEVSDERSAYMTRGHNNARII
                     TLGSEISAPGIAKNIIKGFVEGKYDGGRHQVRVDMLNKMC"
     gene            7353..8126
                     /locus_tag="ACGEUI_001558"
     CDS             7353..8126
                     /locus_tag="ACGEUI_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004270277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8166379.1"
                     /translation="MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLA
                     DKGLLIRTHGGAQYLDYSSAKDEGHEKTHTEKKVLQTTEKKQIAQRAKNIIKDGETVF
                     IGPGTTLEHLAVELKNRNIRVITNSLPVFLILHPSTTIDLILIGGECREITGAFVGAI
                     AINNLETLTFSKAFVSANAVYNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESY
                     DFYNFYQLDQVDLIVTDKNVTTDIIEKYKKFSKIIIADK"
BASE COUNT         2705 a         1677 c         1407 g         2829 t
ORIGIN      
        1 gagtaaaaag tgcaaaaatg acttttttca tattttttag taagattttt gagatgtcac
       61 ataagaggtc actttataac aaatagccct ctgatataaa aaacaaaact cgttctttac
      121 agctatttta caatctaatc gcttctcgtt ataacatcaa tctctaatca gaaaatggtt
      181 actttactta tttaatcagg tcaggagttt gaatgacaga agtcaaatat ctgaaaaagg
      241 atgttaaata aaactttcaa aacccatttt catcaactaa aaacacttat aacccgatct
      301 tacaactaat ggattaccat caacagcaaa taatactatg cttgaaaaat gtcaacatca
      361 tctacttttt gtgactataa ttcagtaaac gaacatgacg tgtaaaaatg tattaaagaa
      421 gtatctcaaa atcattcaat cacttgagtt tctgctactt ttttatacca gtaggcactc
      481 ttcttaggat aacgttcttg agtttcaaaa tcaacataga agagaccgta acgtttttca
      541 tagccatttg accatgaaaa gacatccatc agtgaccaca taaagtatcc tttaacattt
      601 gcaccatcag aaatagcatc agaaataact tctaagtgtt ttttcacata atcgatacgt
      661 ccaccatcat agacagtatt atctacaaac tcatctttgt agccaagacc attctctgta
      721 atgtaaattt tcttgtaatt aggataatcg gctttgacac gcatgatttg atcatataag
      781 ccttgtggga agataatcca gtcccagtcc gtttttggaa catcaacggg tgcttttctt
      841 cgaccaacac ccttgatttg gtatttagag ctgccttttt cacccttgcc attgtgaatg
      901 atttcagtct caccatcaaa agcttgcatc caatcactca tatagtagtt aataccaagg
      961 aaatcattca aatcttttgc ggcgtctagt gcggcaaaat cttcttcgcg aagatcaagt
     1021 tcaccgccat tcacctcaag gatatggtta acaccttcca ttgttttatc tgaatactta
     1081 ccaagataag tagcatcaag gataaattta ttatggatga tatcttcaag ttcagctgct
     1141 ctaacatcat caggattgtt agcgtcaaat ggatacttag ttggaagtgc atggacaaca
     1201 ccaatttcac ctgaataacc actatcttta aagagtttga ctgcacgagc atgagagacc
     1261 atcatgttat ggtgtgattg gaaaacttta gcaagatcat attggatacc tggagggaat
     1321 ttaccaacta agtattggcc atcaccaata ggcccaattt cgttaaatgt tgtccaatag
     1381 ttaacttctg agaattcttt aaaacaaaat tctgcataat ttacaaaatg ttcaatgttc
     1441 tcacgattga ggaagtcacc atccgagtgg agagcttctg gggtatcaaa atggtgaagt
     1501 gtaacaaaag gctcaacatg acgcttatga cactctgcaa aaagattgtg gtagtattct
     1561 actcctttag ggttaacttc tccttttcct gttggaaaaa tacgagacca ggcaatagag
     1621 atacggatgc cgttgacacc aaattcttca ctaagtttca aatcgacagg gtaacgatta
     1681 taaaaatcac ttgctggctc agctgtgtac caatagttgt cttctaagta tttatcccaa
     1741 gctactggtc ctttaccatc tgtgtgggta gcgccttcag cctggtaagc agctgtagca
     1801 ccaccaaaaa taaaatcttt aggtaatgtt ttagtcatct cagtttctcc ttgaatatct
     1861 ttcaaaaata aagttcccaa aacaattttt gtgtttgaga actttacgac atttaaactg
     1921 ttttcttaat tttcaaactg tgcttttaca aaatcaagag ctccttgacc atcacgggtc
     1981 aatttgatgt attgagcccc ttctgtttta gctaatttaa tatcaagttt atcagtttcc
     2041 actttcatat cttcatagtt agaagctact tgaggtgcaa ggataacaag ttgatattgt
     2101 ggtaaaatct cacggtgagc accataactt cctgctgctg cagtgacagg tgcaccatat
     2161 tctttagctg ctttattcaa agcatttgca agcaaaccac ttgtaccacc accggcacaa
     2221 agaacgagaa cattagtttg atcagtgatt tctttggttt ctttagctgc tgatttttca
     2281 agaatagcat cagctttttt agtatcaaag tttgctgcta ctttttcttt gagggcatct
     2341 gatgaattcg tacctgctgc ttcttcagca agaatttgct catcatatac tttcaagaat
     2401 gggtagtaga tgatcacatc aacgacaatt aacaaagctg ccaaaatgaa tgctagaact
     2461 tggaagtttg taccaagaat gagacctaat ggagctggtg tagtccaagg taagtttgca
     2521 gtgaaactgt tcattccaag tgtgtcaatg aagaatttga aaatccatac gttgacaatc
     2581 ggtgccaaga taaatggaat aaagaaaatt gggttcaaaa caagtggagc accaaacaaa
     2641 atgggttcgt taacaccaaa gaatgttgga acaactgatg cacgtccaat ggctttgtta
     2701 cgttttgatt ttgttaacca catgaacatg aatggaacga cgagagttgc accagtacca
     2761 cccatggtaa cgatgaacat ttgagtccct gaagtcaaaa tcttgtcagc atgttcacca
     2821 gcttgaagta attgaaggtt ggtttcagca tttgcgtaag taatcgctgc aatggctggt
     2881 tcaacaatag atggtccatg aataccaaca aaccagaaga aagcaaatgc accgaagata
     2941 atagtgattc caacataacc atctgctgct tggaacaatg gtgcaagaag tttaccaatt
     3001 gactcggcaa cgttaacgcc taaagtagta tgaacaatca attcaatcac ataaagaagt
     3061 acaactgata atgtaaatgg aatcaaatct ttaaaaactt gagatatatt tggtggcacc
     3121 tcttctggca tgcgaatagt aacattgttt ttcacacagg ctttatagac atttacggta
     3181 ataaaagctg cgatgaaggc cgttaacaaa ccttttgttc ccataaatcc agttaagaaa
     3241 ccaccgtctt ttgctggatt tgctgccata agtaaaaaac ctacaatagc agcaagcatg
     3301 gttgaaataa agttgatttg gttagtgcta ggcaatgagc ggttcattga atcggttaat
     3361 gcttttgcag tcgtaccagc cacaaacaaa cctaaaatac ccattgagta gttgtatggc
     3421 gtcatcaaga gattctcaat atccttactc caatggaagc cccatgcatt tggaacatag
     3481 gcaatcaaga taaagatact tgaaaacaag ataacaggca taccagcgat aaaaccatca
     3541 cgaatagcgc gaagatagat gtttcgagaa attttctcga agaaaggttt ccctttctca
     3601 atctgagcaa ttaatccatt cattattttg ctcctctctt ataaagttca atcatgtgtt
     3661 tcattaaatc ttgtaataaa atggtcgtca ttaaatgatc ttgaccatgc attaaagtga
     3721 cactataagc taagtcatct ccagctgctt ctttttgtag tagacttgtc tgagcattgt
     3781 gagcatcatt caagcagtca ctacctgcag cgattaattc ttctgcttta gcataatctc
     3841 cttcttgcgc tgctttaagc gcttccaaaa attttgaacg ggcatcacca gcataggcta
     3901 cgatttcaaa acctaaaaga gtagtttctt ctctattcat tgtagtttcc tccgaaaata
     3961 atgttacatt ttttcagtcc aaggacttgc agttttatct agtactttgt ttaattcgtc
     4021 aatattttta aaaccttctg tacgcaacca ttcacgagct gcttgtggtc cttcttcgat
     4081 atagactttt acagatcctg cccaggtagc acgtccacat aagacaccgt tgaatttagc
     4141 acctgattct gctgcaaaca ccaaagtatc ttggaacaat ttggcagaaa caccagcact
     4201 caaataaatg tatggtaaat cagtagaagc ttcttggtca cggaaagctt gagctgcttc
     4261 ttcttttgta aagagaacct caccatctgc aaagccttct acaaatttca tatttactgg
     4321 aacttcaact ttcaacacgt caactccaaa gcgctctttt gagaaaactt tcattgcttc
     4381 gttaacttta tgagctttaa cttttgcaaa ctcagggctt gcattgtcag caattttctc
     4441 atcataagtt agaatttcta agtagaatgg aatatcttct gcacggcatt cagaaccaat
     4501 acgttcaatg taagcttttt tctgctcgtt aacatcttga tctccatcaa tatcatagta
     4561 aagaaggaat ttaactgctt ctgctccagc ttctttaata cgttttgcag accaaacatc
     4621 taaacaatca ggtaagcgac tagttgttgt tgcatcgtat cctgttttct catatgccaa
     4681 caacaagcct gcttcttctg aacgaacacg acttgctggc aagccatatt caggatcaag
     4741 tagaattgag gatgcaaatg gtgttagttc ttcagaaact aaacttttaa gctcttcaat
     4801 ttgttctact gttggctctt ttgtttgatg ttgagccatc atacgtttga gagctccacg
     4861 ttggtcaaaa gctaaggcag aaataacacc atctacactt agtttttcca tgcttttgcg
     4921 tttgttttca gttaaagtaa ttgtcatttt ttatacctct ttgactttaa tagattgata
     4981 taacttatcg taatttgcca tgttaacatg cccagttgtt ttttcttgcg catttaacat
     5041 ccctaagaca tttgcttttg ttaataaagc tcgatcatct tcatcattcg ctaaacctga
     5101 agcaatacct gccactgtag aatcccctga acctacagcg cttacaattt tgattttagg
     5161 aatgtcaacg ttataaaacg tatccttgtg ctttgcaaat acaccatcag caccaagaga
     5221 aacgattatc cattcaattc catcaaatag ttcatcttgt aagacttctt ttaaggcatc
     5281 aaaatctttt gtcatttctt tacctagaag ttgagaaagt tcttcaagat taggtttgat
     5341 aactgttggt ttactatcac cttttagtac tgcttctaga gcatttccag aacaatccag
     5401 aaccgttttc ttaccattaa gattggcaat cttgattaac ttctgatagt aatcatctgg
     5461 cataccagca ggcaaactgc ctgagagcgt cactacgtca acatcattca gtaaatactt
     5521 aaaatgatga gtaaatccat caatttcatc tacacttaac atcggtcctt tttctaaaat
     5581 ctccgtttga ttaccttcgt gtaaaatggc tatacaagtt ctagtatcac ctgaaatttt
     5641 atagaagcga cttaaaatcg aatcaggaag atgattaatg ataaaatcac cgctttctcc
     5701 acctacacaa cctgtagctt tgactgtctc accgaattca ttaagaactc gggaaacatt
     5761 caagccttta ccaccgggtg tctttgtaac gtctacaact cgattgacag tatcgcattt
     5821 tagctcatcc aaagggtagg agacatcaat agctggattt aaagtaactg ttaaaatcac
     5881 aatagctcct ttttctatat atatccctat agtaatttat tcaaatatta gtcgtgatat
     5941 tcaccacggt cccatttttc taggaattca tcaaagaagt gtgggttgtt ttgatcttgg
     6001 ctagcatgtt gcaagctatc cattttttca atccattttt tattttcttc agttggtttg
     6061 tattccgcgg ccaagaaagc atcgataata tcattcatca ataaaccacc gataattttt
     6121 ccaccaaaac caataacgtt tgcgtttagc tcttcttttg agtagatagc agatgtcata
     6181 tctctaacga gtgctgaacg aattccagga actttgttta cagcatttgt gataccaaca
     6241 ccagtaccac agataacaac acctagttct gcttcaccac ttgctactgc ttcaccaact
     6301 tttttaccaa aaattggata gtgtgtgcga acattatcat acgtaccaca atcaataact
     6361 ttatgacctt gtgattttag gtaatccaca acagcgattt tttcataagt aacaatatgg
     6421 tcacaaccta cagcaatttt catttttgtt ctccttgttt taacctattt caatcaatta
     6481 gcacatttta ttaagcatgt cgacacgaac ttgatgacgt ccaccatcat atttaccttc
     6541 gacaaagcct ttgataatgt tctttgcaat tcctggagca ctaatttcag aaccaagcgt
     6601 gatgattcga gcattgttgt gtcctcttgt catgtatgct gagcgctcat ctgatacttc
     6661 agctgccacc attcctttga ttttagtagc taccatgaaa gaaccaacac catatgcatc
     6721 aatgacaata cctaagttat catcggatgc attaacttct tttacaacag ctaaagttgt
     6781 atctacaaag tcacgtactt cgttttcact aacatcaacg acttcgtatt taccttcttt
     6841 aaggtaatct ttgataactt ctttaagttc ttgacctgct ttgtcagcac cgataataat
     6901 tgccatttta tttctcctat gatttagaat aagtaaatgt ggaaaaccta cgtagatagt
     6961 taattaacga tttgcaacat cttttgaaat aacgctttca ttatttctat gacctaatca
     7021 tacacccacc aaacataaaa gtcaacattt ttttgtttta tttttataaa aaataaacaa
     7081 aatatgttta tttttttcat ttttacgtta ttatcctaat gtttcttgtg tttctttttc
     7141 atttcataaa tccctttaac agccactcgt aacctatcat atattacgaa acccttatct
     7201 ctatttattg taaacgtttt atttaaaaca ttttctattt tgttaatttt tgttttttct
     7261 aatgtttatg tttgttttgt ttgatttttt tattgatatt atgttatgat acctttaata
     7321 gttataccct aagacactga taggagataa gaatgaaaaa gaaagagcgt catgaaaaaa
     7381 ttctcgatat cttaaaagtt gacggtttta taaaagtaaa agatattatc gatgaaatga
     7441 acatctcaga tatgaccgct agacgagatc tcgatacatt ggcagataaa ggattactta
     7501 tcaggaccca tggtggtgct caatatttgg attattcttc agcaaaggac gaaggtcacg
     7561 agaaaacaca cactgaaaag aaggtcttac agactaccga aaaaaaacaa attgcacaaa
     7621 gagctaaaaa cattattaaa gatggcgaaa ctgtctttat aggtccaggg acaacattag
     7681 agcatctagc tgtcgaattg aaaaatagaa atatcagagt tattacaaat agccttcctg
     7741 tttttttaat tttgcacccg agcactacta ttgatttgat tttaattggt ggtgaatgtc
     7801 gtgaaattac tggtgctttt gttggtgcta ttgccattaa taacctagaa acattaacat
     7861 tttcaaaagc ttttgtcagc gcaaacgcag tttacaataa ttctattgcg acgtacagtg
     7921 atctcgaagg agaagttcaa agggttgcat tgaataatgc tgttgagaaa atccttctag
     7981 ttgatagtac caagtttgaa agttatgact tctataattt ttaccaacta gatcaagtgg
     8041 acttaattgt gactgataaa aatgtgacaa ctgatatcat agaaaagtac aagaaattct
     8101 caaaaatcat aatcgctgac aagtaaagga cctactcata ttaaaaagca cttcagggtt
     8161 tagatatatc ccctgtcaag taaacaggga gcatatctga aagtagtgtt tgtgttataa
     8221 agaagtttct tgctaactta ttttctgttt tttttcagac ttatcagtct tgtactgttc
     8281 ctttttctga tgaataacgt ggaaataggc agacagctat atatttcacc tctagaatgg
     8341 ttgccattac tgtcatcaag acatgcttgt tctttatcgt atggagtaaa gctttctttt
     8401 agccatcaac atacctctta agtagatttt ggttcgttac catgtctact taagaggtgt
     8461 cttcaaagcg ctttaaaacg ttgctataac agtatattaa cgttttgaga attgtgaagc
     8521 tttacgagct tttttaagac ctggtttttg gttgatattc tgatatacat aaagatactt
     8581 ataaaatcaa cctattcctg gttcaatcaa tcaaaatc
//
LOCUS       DAXWIQ010000059         8405 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000059 DAXWIQ010000000
VERSION     DAXWIQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.059"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            66..2585
                     /gene="secA"
                     /locus_tag="ACGEUI_001559"
     CDS             66..2585
                     /gene="secA"
                     /locus_tag="ACGEUI_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFF8166380.1"
                     /translation="MANILRKVIENDKGELRKLEKIAKKVESYADQMASLSDRDLQGK
                     TLEFKERYQKGETLEQLLPEAFAVVREAAKRVLGLFPYRVQIMGGIVLHNGDVPEMRT
                     GEGKTLTATMPVYLNAIAGEGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PAEKREAYNCDITYSTNSEVGFDYLRDNMVVRQEDMVQRPLNFALVDEVDSVLIDEAR
                     TPLIVSGAVSSETNQLYIRADMFVKTLTSVDYVIDVPTKTIGLSDSGIDKAESYFNLS
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEDGEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKEGVRIQEESKTSASITYQNMFRMYKKLAGMTGTAKTEEEEFREVYNMRIIP
                     IPTNRPIARIDHTDLLYPTLESKFRAVVEDVKTRHAKGQPILVGTVAVETSDLISRKL
                     VEAGIPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGSDRIKAFLDRMKLDE
                     EDTVIKSGMLGRQVESAQKRVEGNNYDTRKQVLQYDDVMREQREIIYANRRDVITANR
                     DLGPEIKAMIKRTIDRAVDAHARSNRKDAIDAIVTFARTSLVPEESISAKELRGLKDD
                     QIKEKLYQRALAIYDQQLSKLRDQEAIIELQKVLILMIVDNKWTEHIDALDQLRNAVG
                     LRGYAQNNPVVEYQAEGFKMFQDMIGAIEFDVTRTMMKAQIHEQERERASQRATTAAP
                     QNIQSQQSANTDDLPKVERNEACPCGSGKKFKNCHGRKSFS"
     gene            2701..3057
                     /gene="acpS"
                     /locus_tag="ACGEUI_001560"
     CDS             2701..3057
                     /gene="acpS"
                     /locus_tag="ACGEUI_001560"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFF8166381.1"
                     /translation="MIVGHGIDLQEISAIEKVYQRNPRFAQKILTEQELAIFESFPYK
                     RRLNYLAGRWSGKEAFAKAIGTGIGRLTFQDIEILNDVRGCPILTKSPFKGNSFISIS
                     HSGNYVQASVILEDKK"
     gene            3054..4154
                     /gene="alr"
                     /locus_tag="ACGEUI_001561"
     CDS             3054..4154
                     /gene="alr"
                     /locus_tag="ACGEUI_001561"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFF8166382.1"
                     /translation="MISSFHRPTVARVNLQAIKENVASVQKHIPLGVKTYAVVKADAY
                     GHGAVQVSKALLPQVDGYCVSNLDEALQLRQAGIDKEILILGVLLPNELELAVANAIT
                     VTIASLDWIALARLEKKECQGLKVHVKVDSGMGRIGLRSSKEVNLLIDSLKELGADVE
                     GIFTHFATADEADDTKFNQQLQFFKKLIAGLEDKPRLVHASNSATSIWHSDTIFNAVR
                     LGIVSYGLNPSGSDLSLPFPLQEALSLESSLVHVKMISAGDTVGYGATYTAKKSEYVG
                     TVPIGYADGWTRNMQGFSVLVDGQFCEIIGRVSMDQLTIRLPKAYPLGTKVTLIGSNQ
                     QKNISTTDIANYRNTINYEVLCLLSDRIPRIY"
     gene            4241..5752
                     /locus_tag="ACGEUI_001562"
     CDS             4241..5752
                     /locus_tag="ACGEUI_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8166383.1"
                     /translation="MNKNKLLRVAMLLSLLAPTAESMTVLAQDVMLETHKATTNETSD
                     SSSKEENNKNAAPTTSDKTDQGPLDASAETNSNSLVNADDKKRSDSSQSAIGSSDNKA
                     EAENQVDDKSTDHSKSTDHSKPTDQPKPSPSKVDTAPASSLSKQLPEARTPIQSLSPY
                     VSDLDLSEIDIPSVNTYAAYVEHWSGKNAYTHHLLSRRYGIKADQIDSYLKSTGIAYD
                     STRINGEKLLQWEKKSGLDVRAIVAIAMSESSLGTQGIATLLGANMFGYAAFDLDPTQ
                     ASKFNDDSAIVKMTQDTIIKNKNSNFALQDLKAAKFSRGQLNFASDGGVYFTDTTGSG
                     KRRAQIMEDLDKWIDDHGGTPAIPAELKVQSSASFASVPAGYKLSKSYDVLGYQASSY
                     AWGQCTWYVYNRAKELGYQFDPFMGNGGDWKYKVGYALSKTPKVGYAISFAPGQAGAD
                     GTYGHVSIVEDVRKDGSILISESNCIGLGKISYRTFTAQQAEQLTYVIGKSKN"
     gene            6241..>8405
                     /gene="shr"
                     /locus_tag="ACGEUI_001563"
     CDS             6241..>8405
                     /gene="shr"
                     /locus_tag="ACGEUI_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein Shr"
                     /protein_id="HFF8166384.1"
                     /translation="MKKISKCAFVAISALVLIQATQTVKSQEPLVQSQLVTTVALTQD
                     NRLLVEEIGPYASQSAGKEYYKHIEKIIVDNDVYEKSLEGERTFDINYQGIKINADLI
                     KDGKHELTIVNKKDGDILITFIKKGDKVTFISAQKLGTTDHQDSLKKDVLSDKTVPQN
                     QGTQKVVKSGKNTANLSLITKLSQEDGAILFPEIDRYSDNKQIKALTQQITKVTVNGT
                     VYKDLISDSVKDTNGWVSNMTGLHLGTKAFKDGENTIVISSKGFEDVTITVTKKDGQI
                     HFVSAKQKQHVTAEDRQSTKLDVTTLEKAIKEADAIIAKESNKDAVKDLAEKLQVIKD
                     SYKEIKDSKLLADTHRLLKDTIESYQAGEVSINNLTEGTYTLNFKANKENSEESSMLQ
                     GAFDKRAKLVVKADGTMEISMLNTALGQFLIDFSIESKGTYPAAVRKQVGQKDINGSY
                     IRSEFTMPIDDLDKLHKGAVLVSAMGGQESDLNHYDKYTKLDMTFSKTVTKGWSGYQV
                     ETDDKEKGVGTERLEKVLVKLGKDLDGDGKLSKTELEQIRGELRLDHYELTDISLLKH
                     AKNITELHLDGNQITEIPKELFSQMKQLRFLNLRSNHLTYLDKDTFKSNAQLRELYLS
                     SNFIHSLEGGLFQSLHHLEQLDLSKNRIGRLCDNPFEGLSRLTSLGFAENSLEEIPEK
                     ALEPLTSLNFIDLSQNNLALLPKTIEKLRALSTIVASRN"
BASE COUNT         2763 a         1458 c         1763 g         2421 t
ORIGIN      
        1 cctttcttga actgcctgca tatttatgtt aaaatgaaat gattagatga tcgaaaggaa
       61 agagaatggc caatattcta cgcaaagtta tcgaaaatga caaaggcgaa ctaagaaaat
      121 tagaaaaaat tgcaaaaaaa gtagaatcct acgcagacca aatggcgtca ttgtcggata
      181 gagatctaca aggaaaaaca cttgaattta aagaacgcta tcaaaaaggt gagaccctcg
      241 aacaactatt acctgaagct ttcgcagttg ttcgtgaagc agcaaaacgt gtgttgggct
      301 tgttccctta tcgtgttcaa attatgggag gaattgttct tcataacggg gatgtccctg
      361 aaatgcgtac tggtgaagga aaaactctta cagccaccat gccagtttat ctcaatgcta
      421 ttgctggtga gggtgttcac gttattactg ttaatgagta tctttcaacg cgtgacgcta
      481 ccgaaatggg tgaagtgtat agctggttag gtctttcagt tgggatcaac ctagctgcta
      541 agtctcctgc tgaaaaacgt gaagcttata attgtgatat tacttattca accaactctg
      601 aagtcggttt tgattacctt cgtgacaaca tggttgttcg ccaggaagac atggtacaac
      661 gcccactcaa ttttgccttg gttgatgaag tggactcagt cttaattgac gaggcaagga
      721 cacctttaat tgtttctggt gctgttagct ctgaaaccaa tcaattgtat attcgtgctg
      781 acatgtttgt aaaaactctg acgtcagtgg attatgttat tgatgtgccc actaaaacga
      841 ttggattaag cgattcaggt attgataaag ctgagagtta ctttaacttg agcaatcttt
      901 acgatattga aaatgtagcc ttgactcact ttatagacaa cgcccttcgt gcaaactata
      961 ttatgttgtt agatatcgat tatgtggtca gcgaagatgg tgagattctt attgttgacc
     1021 aatttacagg tcgtacgatg gaagggcgtc gcttctcaga cggcttgcac caagctattg
     1081 aggcaaaaga aggcgttcgt attcaggaag aatcaaaaac cagcgcctca atcacttacc
     1141 aaaatatgtt tcgtatgtac aaaaaattgg caggtatgac tggtactgcg aagaccgagg
     1201 aagaagaatt tcgcgaagtt tataacatgc gtattattcc aattccaacc aatcgtccaa
     1261 ttgcccgtat tgatcacact gatttgcttt acccgacctt agaatcaaag tttagagcag
     1321 ttgtggaaga cgttaaaacg cgtcatgcaa aaggacaacc tatccttgta ggtacggttg
     1381 ctgttgaaac tagtgatttg atttcacgca agctggttga agcagggatt cctcatgagg
     1441 ttttgaatgc taaaaatcac tttaaagaag ctcaaatcat tatgaatgct gggcaacgtg
     1501 gtgctgttac aatcgcaact aacatggctg gtcgtggtac tgatatcaag cttggtgaag
     1561 gtgttcgtga acttggtggg ttatgtgtca ttggtacaga gcgtcatgaa agccgtcgta
     1621 tcgataacca gcttcgtgga cgttcaggtc gtcaaggaga tccaggtgaa tcccaattct
     1681 atctttcatt agaagatgat ttgatgagac gtttcggctc tgatcgtata aaagcctttt
     1741 tggatcggat gaaattagat gaagaagaca ctgttattaa atcaggtatg ttagggcgtc
     1801 aagtggaatc tgctcaaaaa cgggttgaag gaaataacta tgatacccgt aaacaggtct
     1861 tacaatatga tgatgtgatg cgtgaacagc gtgaaattat ctatgctaac cgtcgtgatg
     1921 tcataactgc taatcgagat cttggcccag aaattaaagc tatgatcaag cgtactatcg
     1981 accgtgctgt agatgctcat gcacgtagca atcgtaaaga tgctatagat gctattgtaa
     2041 cttttgctag gacctcgctg gtaccagaag agtctattag tgctaaagag ttacgtggtt
     2101 taaaagatga tcaaatcaaa gagaagcttt atcaaagagc gcttgctatc tacgaccagc
     2161 aactatcaaa acttcgtgat caagaagcta ttattgaatt gcaaaaagta ctcattttga
     2221 tgattgtgga taacaaatgg actgaacaca ttgatgcctt agatcaactt cgtaatgctg
     2281 ttggacttcg ggggtatgct caaaataacc ctgtggtgga ataccaagca gaaggtttca
     2341 agatgttcca agatatgatt ggtgctatcg agtttgatgt aacacgtacc atgatgaaag
     2401 cacaaattca cgaacaagaa cgtgaacgtg caagtcaacg tgccacaaca gcagcacctc
     2461 aaaacatcca atcgcagcag tcagctaaca ccgatgattt acccaaagtg gagcgtaacg
     2521 aagcttgtcc atgtggttca ggcaaaaaat tcaaaaattg tcacggtcgt aaatccttct
     2581 cataagaacg agacacaaca ggacaaagtt aggtatatag aaagaagctg agaataattc
     2641 tcagcttctc actgtttaaa tcttctaaca ttaagatgaa ataaggtttg gtggaaagta
     2701 atgattgttg gacatggaat agacttacaa gagatttcag ctattgaaaa agtttatcaa
     2761 agaaatcctc gctttgctca aaaaatttta actgagcagg aattggctat ttttgagagt
     2821 tttccttaca aacgtcgact caactatttg gcaggacgct ggtcaggtaa ggaagctttt
     2881 gctaaggcta tagggacagg tattgggcgg ttaacctttc aagatattga gattttaaat
     2941 gatgttagag gatgtcccat tctgacaaaa tctccgttta aaggaaatag ttttattagc
     3001 atctcacata gtggcaatta tgtacaggct agtgttattt tggaggataa aaaatgattt
     3061 caagtttcca tcgcccaaca gttgcaaggg tcaacttaca agctattaag gagaatgttg
     3121 ccagtgttca aaagcatatt ccactagggg taaaaacgta tgcagttgtc aaggctgatg
     3181 cttatggtca tggtgctgtc caggtgtcaa aagcactcct acctcaagtg gatgggtact
     3241 gtgtgtcaaa tcttgatgag gctttgcaat tacgtcaagc aggtattgat aaagagattt
     3301 taattcttgg ggttttgctg ccaaatgaat tagagttagc agttgctaat gctattactg
     3361 ttacaatcgc tagtttagac tggatagctt tagctagact ggagaaaaaa gaatgtcaag
     3421 gcttaaaagt tcatgtaaaa gttgattctg gtatggggcg gatcgggctt cgttcttcaa
     3481 aagaagtcaa tttattgatt gatagtctaa aagagttggg tgctgatgta gaaggtattt
     3541 tcactcattt tgccacagct gatgaggcag atgatactaa atttaaccag cagttacagt
     3601 tttttaaaaa gctgatagct ggacttgagg ataagcctcg tttagtacat gctagtaatt
     3661 cagccacaag tatctggcat agtgatacca tttttaatgc tgttcgttta ggaattgtca
     3721 gttatggttt gaatccaagt ggttctgatc taagcttacc gtttccactg caagaggctt
     3781 tatctctaga atctagctta gtgcatgtca agatgatttc agctggtgat acagtcggtt
     3841 atggagctac ttatactgcc aaaaagtctg aatatgtagg gactgtccca atcggttatg
     3901 cagatggctg gaccaggaac atgcaaggct tttcggtgtt agttgatgga caattctgcg
     3961 aaattatagg gcgtgtatcg atggatcaac tgaccatacg acttcccaaa gcatatcctt
     4021 taggaacaaa agtcactttg attggcagca atcagcaaaa aaatatttct acaacagata
     4081 tcgcaaatta ccgtaataca atcaattatg aagttctatg ccttttaagt gaccgtattc
     4141 ctcggatata ttaagaaaat catgtaaaaa taattgcatt gtgcttatct ttaatgttat
     4201 aataaagtga ggacattaga aagaagtgac taattaatat atgaataaaa acaaactatt
     4261 aagagttgcc atgctactaa gtctcttagc cccgacagca gaaagcatga cagtgctggc
     4321 tcaagatgta atgcttgaga cgcataaagc aactacaaat gaaaccagtg attcttcttc
     4381 aaaagaggaa aataataaaa atgcagcacc tacaacatca gataaaactg accaaggtcc
     4441 ccttgatgct tctgcagaaa caaactctaa tagtcttgtt aacgcggatg ataaaaaaag
     4501 aagcgattct agtcagtctg ctataggctc ttcggacaac aaggcagaag cagaaaacca
     4561 ggtagatgat aaatcaactg atcattcgaa atcaactgat cattcgaaac caactgacca
     4621 gcccaaacca tcaccatcta aagttgatac ggcacctgct tcttcattgt cgaaacaact
     4681 gccagaggca agaactccta ttcagtcgtt gtccccttac gtatcagatt tagatttgag
     4741 tgagatagat atcccttctg tcaacacata cgcggcatat gtagagcatt ggagtggtaa
     4801 aaatgcctat acccaccatc ttttatctcg ccgttatggt attaaagctg accagattga
     4861 tagttactta aaatcaacag gcattgccta tgacagcaca cgtattaatg gtgagaagct
     4921 attgcaatgg gaaaagaaaa gtgggctgga tgttcgagct atcgtagcta ttgcgatgtc
     4981 tgagagttct ttaggaactc aagggattgc aactttgctt ggagctaata tgtttggcta
     5041 tgcagctttt gatctagatc cgactcaagc aagtaagttt aatgatgata gtgctattgt
     5101 caaaatgaca caagacacca ttattaaaaa caaaaatagc aattttgcac ttcaagattt
     5161 aaaagcggct aagttttcac gaggtcaatt aaactttgca agtgacgggg gtgtttattt
     5221 tactgatact actggtagtg gtaaacgtcg cgcacaaatt atggaagacc tggataagtg
     5281 gattgatgac catggtggca caccagccat tccagccgaa ttgaaagtgc agtcatcagc
     5341 tagttttgca tctgtgccag caggttataa gctctctaag agttatgatg tcttgggtta
     5401 tcaagcttcg agttatgctt ggggacaatg cacttggtat gtgtataatc gcgccaaaga
     5461 attgggttac caatttgatc cttttatggg aaatggtgga gattggaagt ataaagtagg
     5521 gtatgccctt tcaaagactc caaaagtagg ttatgctatt tcatttgcac cagggcaagc
     5581 gggcgctgat ggcacttatg gccacgtatc aattgtagaa gatgttagaa aagatgggtc
     5641 tattcttatt tcagagtcta actgtatcgg cttaggtaag atttcttatc gtacctttac
     5701 agctcagcag gctgaacagc taacatatgt tattggcaag agtaaaaact aataactaat
     5761 gacttttctt aaggcttaat tttcttagcg ggagttaggc cttttaaact gttggtgtaa
     5821 cacgataaag tttcaagtca ataaatgatt ttgagacaat agcacgatta gggctattgt
     5881 ctttttttat tgtgatgaat atgcagtagt gacatctcat gttgtctcct aacacaatta
     5941 tggagcttat aacgtgttac atgttttgtg aatgaagtaa tgattgaatc agctctagta
     6001 ttaactatcc taaggctagc tttatctcag taatattatt gtagaggaat tcttttatca
     6061 gagatatcct cattatcaat ccaggattat tactagtgtc ttagctcaca tagtggagct
     6121 atttaacaga caattggacc attggaaaca atcttgacaa aataatcaca aagtgataga
     6181 ttaagttcag ttaatataaa agttagctta ttcataaaat ttttagaaag ttgattgaaa
     6241 atgaaaaaaa tcagcaaatg tgcgtttgtg gcaatatctg cccttgttct cattcaggct
     6301 actcaaactg taaaatcaca agagccttta gttcagtcac aactcgtgac aacagtagct
     6361 ttaactcagg ataatcgact tttagttgaa gagataggcc cttacgctag tcaatcagct
     6421 ggaaaagagt attataaaca tattgaaaag attattgttg ataatgatgt ctatgaaaaa
     6481 agcctggagg gcgagcgaac ctttgatatt aactaccaag ggattaagat caatgctgac
     6541 cttattaaag acggtaagca tgaattgact attgttaata aaaaagatgg tgatatccta
     6601 attaccttta ttaaaaaggg cgataaagtg acctttattt cagctcaaaa attaggaaca
     6661 acagatcatc aggattcatt aaaaaaagat gtgctcagtg ataaaacagt gccacaaaac
     6721 caaggcacac aaaaagttgt taaatctggg aaaaatactg ctaacttgtc attaataaca
     6781 aaattgagtc aagaagatgg tgcaatttta tttccagaaa ttgatcgtta ttctgataac
     6841 aaacagataa aagcattgac tcagcaaatc acaaaggtta cagtcaatgg tacagtttat
     6901 aaagatctta tttcagattc tgtaaaagat actaatggct gggtctcgaa tatgacaggg
     6961 cttcatcttg gaacaaaagc tttcaaagat ggagaaaata caatcgtgat atcctcaaaa
     7021 ggatttgaag acgttactat taccgttacc aagaaagatg gtcaaatcca ttttgtatct
     7081 gccaaacaaa aacaacatgt gactgctgaa gacagacaat caacaaagtt ggatgtcacc
     7141 actttggaaa aagctatcaa agaagcggat gcgattattg ctaaagaaag caacaaagac
     7201 gcggtcaaag atctggctga gaaacttcaa gtcatcaagg attcttacaa agaaatcaaa
     7261 gatagtaagc tactcgccga tactcatcga ctgttaaaag ataccatcga gtcttatcaa
     7321 gcaggtgagg tttctattaa caatctcaca gaaggaacct atacgctaaa ctttaaagct
     7381 aataaagaaa actcagaaga gtcctccatg cttcaaggtg cttttgataa aagagccaaa
     7441 ttagtggtta aagcagatgg tacaatggaa atttccatgc ttaatactgc tttgggacaa
     7501 tttttgattg acttttctat tgaaagcaaa gggacctacc cagcagcagt gcgtaaacaa
     7561 gttggccaaa aagatatcaa tggtagctat attcgaagcg aatttaccat gcctattgat
     7621 gatttggata aattacacaa aggtgctgtt ttggtatcag ccatgggagg tcaagaaagt
     7681 gatttaaacc actatgacaa atacaccaaa cttgacatga cctttagtaa gaccgttacc
     7741 aaaggctgga gtggttatca ggtagaaact gatgataaag aaaaaggggt tgggactgaa
     7801 cgtcttgaaa aagttttagt taaacttggc aaagatttag acggcgatgg taaattatca
     7861 aaaacggaat tagaacagat tcgaggcgag ttgcgtctag accattacga gttaactgat
     7921 atttctttat tgaaacatgc taaaaatatt acagaactac atctggatgg aaaccaaatt
     7981 acggaaattc caaaagagtt atttagtcaa atgaagcaac ttcgatttct taacttaaga
     8041 agtaatcatt taacttatct agacaaagat acatttaaaa gcaatgctca attaagagaa
     8101 ctctacttat caagtaactt tattcactct cttgaaggag gactattcca gtcgcttcat
     8161 cacctggagc aacttgatct ttccaagaat cgtattggcc gactttgtga taacccattt
     8221 gaaggattgt ctcgtctgac ttcattaggt ttcgcagaaa atagtcttga ggagatacct
     8281 gaaaaagcgc tagagcctct aacatcactt aattttatcg acttatctca aaataattta
     8341 gcactactgc caaaaacaat agaaaaattg cgcgccttaa gcactattgt ggcaagtaga
     8401 aatca
//
LOCUS       DAXWIQ010000060         7940 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000060 DAXWIQ010000000
VERSION     DAXWIQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.060"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            16..882
                     /locus_tag="ACGEUI_001564"
     CDS             16..882
                     /locus_tag="ACGEUI_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFF8166385.1"
                     /translation="MKMKKKFFLLSLLALSTFFLSACSSWIDKGESITAVGSTALQPL
                     VEAVADEFGSSNLGKTVNVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKDGIDASKLVD
                     HQVAVAGLAVIANPKVKVSNLSSQQLQKIFSGEYTNWKQVGGEDLAISVINRAASSGS
                     RATFDSVIMKGVNAKQSQEQDSNGMVKSIVSQTPGAISYLSFAYVDSSVKSLQLNGFK
                     ANAKNVATNDWPIWSYEHMYTKDKPTGLTKEFLDYMFSDEVQQNIVTHMGYISINDME
                     VVKSHDGKVTKR"
     gene            893..1828
                     /gene="pstC"
                     /locus_tag="ACGEUI_001565"
     CDS             893..1828
                     /gene="pstC"
                     /locus_tag="ACGEUI_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFF8166386.1"
                     /translation="MENQELAKKLASPSKNSRLETFGRTITFLCLALIVFIVAMILIF
                     VAQKGLSTFFVDKVNLFDFLFGKEWQPSVKNAAGIPYLGALPMITGSFLVTILSAIIA
                     TPFAIGAAVFMTEISPKYGAKLLQPAVELLVGIPSVVYGFIGLQVIVPFMRSIFGGTG
                     FGILSGVCVLFVMILPTVTFMTTDSLRAVPRHYREASMAMGATRWQTIWRVVLNAARP
                     GIFTAVIFGMARAFGEALAIQMVVGNSAVMPSSLTTPAATLTSVLTMGIGNTVMGTVQ
                     NNVLWSLALVLLLMSLAFNSLVKLITKERKRNYER"
     gene            1818..2705
                     /gene="pstA"
                     /locus_tag="ACGEUI_001566"
     CDS             1818..2705
                     /gene="pstA"
                     /locus_tag="ACGEUI_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFF8166387.1"
                     /translation="MNAKKVDKVATGTLYTIAGIIVAILASLILYILVRGLPHISWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSTGAGIYLAEYAKKGPVTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLLHVH
                     HTQREAGLALGLSRWETVFYVVIPEALPGMVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATLVSAGSAAVLLIFILIF
                     NFSAHFIGKKLHSKMTAAK"
     gene            2721..3524
                     /gene="pstB"
                     /locus_tag="ACGEUI_001567"
     CDS             2721..3524
                     /gene="pstB"
                     /locus_tag="ACGEUI_001567"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFF8166388.1"
                     /translation="MTEYNWNERHIITFPEETLALATKDLHVYYGAKEAIKGIDMQFE
                     KHKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGEILYQGIDVNRKDMNVYEIRKH
                     LGMVFQRPNPFAKSIYKNITFAHERAGVKDKKVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGNLIEYDKTRNIFQNAQCQSTNDYVSGHFG"
     gene            3537..4295
                     /gene="pstB"
                     /locus_tag="ACGEUI_001568"
     CDS             3537..4295
                     /gene="pstB"
                     /locus_tag="ACGEUI_001568"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFF8166389.1"
                     /translation="MTEPILQIRDLSVYYNQKKTLKDVSLDLYPNEITALIGPSGSGK
                     STLLRSINRMNDLNPEVTITGSIVYNGHNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRLKGIRDKSILDNAVESSLKGASIWNEVKDRLHDSAVGLSGGQQQRVCIA
                     RVLATSPRIILLDEPTSALDPISAGKIEETLLLLKKDYTLAIVTRSMQQASRLSDRTG
                     FFLEGDLLECGPTKAMFMNPKRKETEDYISGKFG"
     gene            4363..5016
                     /gene="phoU"
                     /locus_tag="ACGEUI_001569"
     CDS             4363..5016
                     /gene="phoU"
                     /locus_tag="ACGEUI_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFF8166390.1"
                     /translation="MLRMKFEEELDKLHNQFYSMGTEVLAQINKTVRAFVSHDRELAK
                     EVIEEDDTINNFETKLEKKSLEIIALQQPVSNDLRMVITVLKASSDIERMGDHAASIA
                     KATIRMKGEERIPVVEEQINLMGKAVKQMVEEALNAYINADDTKAYEIAASDEIIDQY
                     FRNIQTLAVEEIRKSPDAVFAGKEYFQVLMYLERIGDYARNICEWIVYLKTGKIIEL"
     gene            5221..7758
                     /locus_tag="ACGEUI_001570"
     CDS             5221..7758
                     /locus_tag="ACGEUI_001570"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HFF8166391.1"
                     /translation="MKTVEHLIETFVPENYNIFLDINRQTKTFTGNVAINGEALDNHV
                     AFHQKDLDIKSILLDNEAVIYQVDNDNEVVRVELPETGMMTLVIEFSGSITDNMTGIY
                     PSYYTKNGEKKEVISTQFESHFAREAFPCIDEPQAKATFDLSLTFDQEIGEIALSNMP
                     EVNIDRREETGLWTFDTTLRMSSYLLAFALGELHGKTVESKKGTTVGVYATTAHPLSS
                     LDFSLDIAVRVINFYEDYFGVHYPIPQSLNIALPDFSSGAMENWGLITYREIYLLVDE
                     NSTVQSRQQVALVIAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIEAIEPSWK
                     IIEDFQTGGIPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRF
                     IGDRDFAIGLHHYFEKYQYRNTVGRDLWNILSDTSGKDVAAFMDAWLEQPGYPVLTAR
                     LENDQLILSQKQFFIGKGEEKGRLWPIPLNTNWHGLPETLTEAEMVIPNFSQLAAENE
                     GALRFNIDNTAHYITDYQGSLLDALVTELAQLDNTSALQVIQERRLLADSGLISYAEL
                     VDLIAQLDDSKSYMVAEAVQQVVSGLKRFIDEGSLAEKSFNRLVTTIYQEDFNQHGFE
                     KKADESDEDEMVRQVALGRLWLAENQTIIDGLRTIFEAYQNNIASIPAAVRRLVLANQ
                     MKYFETDSLVDIYFETYVATTDNNLRSDLTVAFSQTKQPTTIRRILVSLKDKDIIKPQ
                     DLSYWYNALLGQSFTQDIIWEWARENWDWIKSALGGDMSFDKFVIYPASNFKTPKHLA
                     EYKSFFEPKLDDMAISRNITMGINEIEARVALITKEKEAVIAALSHY"
BASE COUNT         2414 a         1390 c         1631 g         2505 t
ORIGIN      
        1 aataggaaat aaataatgaa aatgaaaaaa aaattctttt tgttaagtct tttggcccta
       61 tcaactttct ttttatccgc atgttctagc tggattgata aaggtgagtc aataaccgct
      121 gtaggatcaa cagcactaca acccttagta gaagcagtag ctgatgaatt tggaagcagt
      181 aatctaggca agactgtcaa tgttcaaggt ggtggttcag gtacagggtt gtctcaagtt
      241 caatcaggag ctgtccaaat tggaaatagt gatgtctttg cggaagaaaa agatggtatt
      301 gatgcttcta aattagttga tcatcaagta gctgttgcag gacttgcagt tattgccaat
      361 cctaaagtca aggtttccaa tctcagtagt cagcagttgc aaaagatttt ttcaggagaa
      421 tataccaatt ggaaacaagt tggaggagaa gatcttgcga tttcagtgat caaccgagca
      481 gcaagttctg gctcacgagc aacctttgac agtgttatca tgaaaggggt caacgctaaa
      541 caaagtcaag agcaagactc caatgggatg gttaaatcga ttgtttcaca aacaccaggt
      601 gccatttctt acctttcctt tgcctacgtt gattcatctg ttaaatcttt gcaattaaat
      661 gggtttaagg caaatgctaa gaacgtggct acaaatgatt ggccaatctg gtcctacgaa
      721 cacatgtata ccaaagataa accaacaggg ttgaccaagg aatttcttga ttatatgttt
      781 tcagatgaag tacaacagaa cattgttaca catatgggat atatttcgat aaatgatatg
      841 gaagtggtca aatctcatga tggaaaagta acaaaaaggt aatggaggaa ttatggaaaa
      901 tcaggaattg gctaaaaaat tagcctcacc atcaaagaat tcaaggcttg aaacctttgg
      961 ccgcaccatt acgtttttgt gtctggcttt gattgttttt atcgttgcga tgattctgat
     1021 cttcgttgct caaaaagggt tgtcaacctt ctttgttgac aaggttaatc tttttgattt
     1081 cttgtttgga aaggagtggc aaccaagtgt aaaaaatgcg gccggcattc cttatcttgg
     1141 tgctctgcca atgattacag gatccttttt ggttaccatt ttatctgcta ttattgccac
     1201 cccatttgcc attggcgcag ccgtttttat gactgaaatc tcacctaaat atggcgctaa
     1261 attattacag cctgcggttg agcttttggt tggaattcct tcggttgttt atgggtttat
     1321 cggtttgcaa gtgattgttc cttttatgcg ctctatcttt ggtggcacag gttttggaat
     1381 cctatctggg gtctgtgtct tgtttgttat gattttacca acagtgactt ttatgacaac
     1441 agacagtctg cgggcggtgc ctcgtcatta ccgcgaagcg tctatggcta tgggagcaac
     1501 acgttggcaa accatttggc gtgttgtcct taatgcagct cgtccaggaa tttttaccgc
     1561 tgttattttt ggaatggcaa gagcttttgg agaagccttg gctatccaaa tggtagtcgg
     1621 taactctgct gtaatgccaa gttcactaac gacaccagcc gcaaccttaa cgtctgtctt
     1681 gacaatgggt attggtaaca cggttatggg aacggtccaa aataatgtgc tttggtccct
     1741 tgccctagtc ttattattaa tgagtctggc cttcaattcc cttgtcaaat taatcacgaa
     1801 agaaagaaag agaaattatg aacgctaaaa aagtcgataa agtagcaact ggtactcttt
     1861 ataccattgc tggaattatt gtagctattt tagcttcctt aattctatat atccttgtcc
     1921 gtggcttgcc acacatcagc tggtcctttt taacaggaaa atcgtcttcg tacgaagctg
     1981 gtgggggaat tggtatccag ttgtataatt ccttcttcct attgattgtt actttaatta
     2041 tttccattcc tttatcaact ggagcgggga tttacttggc tgaatatgcc aaaaaaggac
     2101 ctgttaccaa ctttattaga acctgtattg agattctgtc ttccctacca tctgtggttg
     2161 tggggctctt tggttacttg attttcgttg tgcagtttga atatggcttt tctattattt
     2221 caggggctct tgctttgacg gtctttaatc ttcctcaaat gacccgtaat gttgaagata
     2281 gtttactgca tgttcatcat acccaaagag aagcaggatt agctttaggc ctgtcacgct
     2341 gggaaacggt tttttatgtg gttatcccag aagctctccc gggaatggta acaggtattg
     2401 tcttagcttc aggtcgtatt tttggtgaag cggcagcgct tatctatact gctggtcaat
     2461 cagcaccagc tctggattgg tcaaattgga atccacttag tgttactagt ccaatctcta
     2521 ttttccgtca atctgagacc cttgctgttc atatttggaa agtcaatagc gaagggacaa
     2581 ttccagatgc tactcttgta tccgcaggaa gtgcagcagt gctattaatt tttatcctta
     2641 ttttcaactt ttcagctcac tttattggga agaaacttca ttctaaaatg acagcagcga
     2701 aataagatat tggagaaatc atgacagaat acaattggaa tgaacgtcat atcatcactt
     2761 ttcctgaaga aacgcttgct ctagctacca aggatttaca tgtttactat ggtgcgaaag
     2821 aagctatcaa agggattgat atgcagtttg aaaaacataa aataacagcc ttgattggtc
     2881 catctggttg tggcaaatca acttatcttc gtagtcttaa ccgcatgaac gataccattg
     2941 atattgctcg ggttacaggt gagattcttt atcagggcat tgatgttaat cgaaaagaca
     3001 tgaatgttta cgagattcgc aagcacttag gaatggtttt tcaaagaccc aatcctttcg
     3061 ctaaatccat ttataaaaac attacctttg cccatgaaag agctggtgtg aaagacaaaa
     3121 aagttttaga tgaaattgtt gagacttcac tgaaacaagc cgcgctttgg gatcaagtta
     3181 aagatgacct tcacaaatca gccttcacct tatcaggggg tcagcaacaa cgcttgtgta
     3241 ttgctagagc catttctgtc aaacctgata ttctattaat ggatgaacca gcctctgccc
     3301 ttgacccgat tgcaacgatg cagctggaag aaaccatgtt tgaattgaag aagaattaca
     3361 cgattattat tgtgacccac aatatgcaac aagcagctcg tgccagtgat tacactgcct
     3421 tcttctattt aggcaacctc attgaatatg ataagacacg taatattttc caaaatgcgc
     3481 aatgtcagtc aacaaacgac tacgtttcag ggcactttgg ttagaaaggt aatatgatga
     3541 cagaacctat tttacagatc agagaccttt ctgtttacta caatcaaaag aaaacgttaa
     3601 aagatgtctc tttggacctt tatccaaatg aaataacagc tttaattggt ccttctggat
     3661 ctgggaaatc aacgctcttg cgttctatca accgtatgaa tgatttgaat cctgaagtaa
     3721 ccattacagg ctctatcgtc tataacggac acaatattta tagtccaagg acagatacgg
     3781 ttgatttacg caaagaaatt ggtatggtat tccaacaacc caacccattt ccgatgtcta
     3841 tttatgagaa cgtggtttac ggccttcgct taaaaggtat cagagataaa tcaatcttag
     3901 acaatgctgt tgaaagctct cttaaagggg cttccatttg gaatgaagtc aaagatcgtt
     3961 tacatgattc tgcggttggc ttatcaggcg gacagcaaca gagagtttgc atagctagag
     4021 ttcttgctac cagccccaga ataatccttt tagatgaacc tacctcagct cttgatccta
     4081 tctcagctgg gaaaattgaa gaaacattat tgttattaaa aaaagattat acactggcta
     4141 ttgtgacccg ctcaatgcag caagcctcac gtttatcaga tcgaacaggt tttttcttgg
     4201 agggtgactt acttgagtgt ggtcctacaa aagccatgtt tatgaatcct aagcgcaaag
     4261 aaaccgaaga ctatatttct ggtaaatttg gttgagaaag atgtatatgg ttgacccctc
     4321 tgtgttttta ttataatgac tagattgtag gaagaaaaaa tcatgttaag aatgaaattt
     4381 gaagaagaat tagataaatt gcataaccag ttttattcaa tgggaacgga agtattggca
     4441 caaatcaata aaactgttcg tgcctttgtt agtcatgatc gtgaattggc caaagaagtt
     4501 atcgaagaag atgacactat taataatttt gagacaaagc ttgaaaagaa atcgctggaa
     4561 attattgccc tacaacagcc agtttcgaat gatcttagaa tggttattac tgttttaaag
     4621 gcatcaagtg atattgaacg tatgggtgac catgctgctt cgattgccaa ggccactatt
     4681 cgtatgaaag gtgaggagcg tattccagtg gttgaagaac aaatcaacct tatgggaaaa
     4741 gctgtgaaac agatggtaga agaagccctt aatgcatata tcaatgctga cgatacaaaa
     4801 gcttatgaaa tcgcagcttc tgatgagatt atcgatcaat atttccgtaa tatccaaacc
     4861 ttagctgttg aagaaatccg taagtctcca gatgctgttt ttgcaggaaa agaatatttt
     4921 caagtattaa tgtatttgga acgtattggt gactatgctc gcaacatttg tgaatggatt
     4981 gtttatctta aaactggaaa aattattgaa ttataagaaa ttatgctata atgagactgg
     5041 gaaacagctt gccagtccct ggttttatac gaaattgtgc aagttagaaa atgaactaac
     5101 aagaaaagtt ttctttgctg aatctgattt tgttaggtta acaaatatat gtagacagag
     5161 atgatactta ggtatcatct gttgatctat tatgatttgt tattagaatt aaggagttat
     5221 atgaagacag tagaacatct catcgaaaca tttgtgcctg aaaattataa catcttttta
     5281 gatattaatc gtcagactaa aacatttacg gggaatgttg cgattaatgg tgaagcttta
     5341 gataatcatg tggctttcca ccaaaaagat ttggacatta agtctatttt gctcgataat
     5401 gaagctgtta tttatcaagt ggataacgac aatgaagttg tgcgtgttga attacctgaa
     5461 acaggaatga tgacgcttgt cattgaattt tcaggtagca ttacagataa tatgacaggt
     5521 atttaccctt catattacac taaaaatggt gagaaaaaag aagtcatttc aactcagttt
     5581 gaaagccatt ttgcacgtga agctttccca tgtatcgatg aaccacaagc aaaagctact
     5641 tttgatttaa gcttgacctt tgatcaagaa attggtgaga tagcgttatc gaatatgcca
     5701 gaagtcaata ttgaccgtcg cgaggagact ggtctttgga catttgatac gacactaaga
     5761 atgtcatctt acttgctagc gtttgcgcta ggagaacttc atggaaagac tgtagaaagc
     5821 aaaaaaggaa caactgttgg tgtgtatgcg acaacagcgc atcctcttag ctcgcttgat
     5881 ttttcattgg atattgcagt tcgtgttatc aacttttatg aagactattt tggagtacat
     5941 tacccaatcc cgcaatctct taatattgcg cttccagatt tttcatctgg tgctatggaa
     6001 aattggggtt taatcactta tcgtgaaatt tatttattag tagatgaaaa ttcgactgtt
     6061 caaagccgac aacaagttgc acttgtaatc gcccacgaga tagcacacca atggtttgga
     6121 aacttggtta caatgaaatg gtgggacgat ctgtggttaa atgagagttt tgccaacatg
     6181 atggaatatg tctctatcga ggctattgag ccgtcttgga aaataataga agatttccaa
     6241 acaggcggta ttcctcttgc tttaaaacgt gatgctactg atggtgttca atcagtgcat
     6301 gttgaagtca atcatccaga tgagataaat acattgtttg atcctgctat tgtctatgca
     6361 aaaggtagtc gtttgatgca tatgttacgc cgttttattg gtgatcgtga ttttgccatt
     6421 ggcttacatc actactttga aaaatatcaa tatcgaaata ctgtcgggcg tgatttatgg
     6481 aatattttat cagatacttc aggcaaagac gtagctgctt ttatggatgc ttggcttgaa
     6541 caaccaggtt atccagtttt aacagctcgc cttgaaaacg atcaattgat tttgagtcaa
     6601 aaacaatttt ttattggaaa aggtgaagaa aaaggtcgcc tttggccgat tcctttaaac
     6661 actaattggc atggccttcc tgaaactttg acagaggcag aaatggtcat tccaaacttt
     6721 agtcaattgg cagcagaaaa tgaaggagct ctacgtttca atattgataa tacagctcat
     6781 tacattacag actaccaagg atcattatta gatgccttag tcactgaact tgcccaactt
     6841 gataatactt ctgctttaca agttattcaa gaacgtcgtt tattggcaga tagtggcttg
     6901 atttcctatg ctgaattggt tgacttaatt gctcaattgg acgattctaa gtcttatatg
     6961 gtagcagaag cggttcaaca agtggtctct ggtttgaaac gcttcattga tgaaggaagc
     7021 ttagcagaaa aatcctttaa tcgcttggta accaccatct atcaagaaga ctttaatcag
     7081 catggttttg agaaaaaagc tgacgaatca gatgaagatg agatggtacg tcaggttgcc
     7141 ttgggccgct tgtggctagc agaaaatcag acgattattg acggattgag aactattttt
     7201 gaagcatatc aaaacaatat tgcaagtatt ccagcagctg ttcgtcgctt ggttttagcc
     7261 aaccaaatga aatattttga aacagatagc ttggttgata tttattttga gacttatgta
     7321 gcaactactg ataataattt acgcagtgat ttaacggttg cttttagtca aacaaaacaa
     7381 ccaactacta ttagacgtat tcttgttagt ttaaaagata aagatattat taaacctcag
     7441 gatttatctt attggtacaa tgctttgctt ggacaaagct ttactcaaga tatcatttgg
     7501 gaatgggcta gagaaaattg ggattggatt aaatctgcac ttggtggaga tatgagcttt
     7561 gataaatttg ttatttatcc agcaagtaac tttaaaacac caaaacatct agcagaatat
     7621 aaatctttct ttgaacctaa gctagatgat atggctatta gccgtaatat taccatggga
     7681 attaatgaaa ttgaagcacg tgttgctttg attactaaag aaaaagaagc agttattgca
     7741 gctctcagtc attactagac cataagcaaa agagttgggg gactttccag ctcttttttg
     7801 tagtgagcta gcttcctttc ttagttccgt ctataactaa atgattatcc atagtttaat
     7861 tcaaaaggag attgcacttt aaagcgaagg ctattagcct cttaaatact tttttaccag
     7921 gtggtgaata agaacaatta
//
LOCUS       DAXWIQ010000061         7823 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000061 DAXWIQ010000000
VERSION     DAXWIQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.061"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(<1..272)
                     /locus_tag="ACGEUI_001571"
     CDS             complement(<1..272)
                     /locus_tag="ACGEUI_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166392.1"
                     /translation="MTIDEALQNLRDNFNKIMNVLKNDWKALLFLAIAIFGMMVTVSY
                     FSYRDARQYYESQITGLRTQLSRTQKQLKRASEDRARQTKRIAELT"
     gene            complement(269..439)
                     /locus_tag="ACGEUI_001572"
     CDS             complement(269..439)
                     /locus_tag="ACGEUI_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166393.1"
                     /translation="MKRYIEVKDEWKSATDHLNDFIDKNKYAKVTVVGYQVVQLSPYG
                     RDLTYILAEVEE"
     gene            complement(436..672)
                     /locus_tag="ACGEUI_001573"
     CDS             complement(436..672)
                     /locus_tag="ACGEUI_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HFF8166394.1"
                     /translation="MIEELVHKPKHYNHGEIDLIESWYKTYPWDQFVAIMESHIDKYI
                     KRHRYKGNATQDLEKALEYTKRLIEYWLIESVEE"
     gene            complement(672..917)
                     /locus_tag="ACGEUI_001574"
     CDS             complement(672..917)
                     /locus_tag="ACGEUI_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166395.1"
                     /translation="MKRHRQWHNDIKYTPRSYDNLLPYDISELLIAHRCKIKMSDDVL
                     ADKIGIYTWQLKALLERRILPNESVCKTIVNYLREVE"
     gene            complement(914..1270)
                     /locus_tag="ACGEUI_001575"
     CDS             complement(914..1270)
                     /locus_tag="ACGEUI_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166396.1"
                     /translation="MTLVDDFYKQMEPSIKAFLDDNITIADKEEADRVYRSVKYYKKL
                     NRLPPPDVLEWFQRIYTTKEMIMLIKQSYRLKQKKTDEDDKIYEKWMFKNYGDVKLVK
                     KIKRTDALERARRMGL"
     gene            complement(1267..1707)
                     /locus_tag="ACGEUI_001576"
     CDS             complement(1267..1707)
                     /locus_tag="ACGEUI_001576"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFF8166397.1"
                     /translation="MVKFIIPIEPKPQKRPRFSRWSGAYEDGDMMAWRKQVTDYVKNN
                     YEGPYFDDGLKVDVTFYLKAPELVSKKPSERAKDKTKQKYQDYINELLYVPKKPDLDN
                     LEKAVYDSISKSEVVWTDDNIIVEHTTRKLYSPNPRIEVKINEL"
     gene            complement(1707..1910)
                     /locus_tag="ACGEUI_001577"
     CDS             complement(1707..1910)
                     /locus_tag="ACGEUI_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166398.1"
                     /translation="MANRYRQRIYAVYDGDSFVDVGTKHELAERLGVTADTIVFIASP
                     AHKKRRPDGRHAEFIGYEDELEE"
     gene            complement(1916..2341)
                     /gene="ssb"
                     /locus_tag="ACGEUI_001578"
     CDS             complement(1916..2341)
                     /gene="ssb"
                     /locus_tag="ACGEUI_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8166399.1"
                     /translation="MINNIVLVGRMTKDAELRYTASQVAVATFTLAVNRRFKEQNGER
                     EADFINCVIWRQSAENLANWAKKGALIGVTGRIQTRNYENQQEQRVYVTEVVAESFQM
                     LESRNSQQQSGQDNSSQNDNSQPFGNSNPMDISDDDLPF"
     gene            complement(2334..3008)
                     /locus_tag="ACGEUI_001579"
     CDS             complement(2334..3008)
                     /locus_tag="ACGEUI_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFF8166400.1"
                     /translation="MRKSESITEYAKAFCKAQLEVKQPLKDKDNPFFKSKYVPLENVT
                     EAITKAFANNGISFSQDPTTNAENGYIDVATLVMHTSGEWVEYGPLSVKPTKNDVQGA
                     GSVITYAKRYALSAIFGITSDQDDDGNEASKPNKTNQSQKPTNKTSKGTSFQTPKISN
                     IQVETYKSDLSDIAKATNQNVKELTKWLTDTLKVKSLEDLRTEQIVSTDDLINKLKKR
                     AGQKND"
     gene            complement(3009..3491)
                     /locus_tag="ACGEUI_001580"
     CDS             complement(3009..3491)
                     /locus_tag="ACGEUI_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFF8166401.1"
                     /translation="MAYLYELEGIAAYLESLDLDDETFQNTLDSIDFQSDLENNIEYF
                     VKMLKNAQADVEMYKAEKEAFYKKQKQAEAKVEKYKETIRRAMELSQKKKVDAGMFKV
                     SLRKSKKVEILDETKIPLDYMQEKIEYKPMKSEISKALKSGIDISGVELIETESLQVK
                     "
     gene            complement(3513..3767)
                     /locus_tag="ACGEUI_001581"
     CDS             complement(3513..3767)
                     /locus_tag="ACGEUI_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166402.1"
                     /translation="MVGIRINGELVTFDSNFRDALIFTIDCLRGSEEPMLKQTYQTFK
                     DYTDEDLMDYIETEFDVWPELIVNRKIDSKWSTKQHILDD"
     gene            complement(3748..4101)
                     /locus_tag="ACGEUI_001582"
     CDS             complement(3748..4101)
                     /locus_tag="ACGEUI_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166403.1"
                     /translation="MVMPLDELEKRIFCYLPIGSNMMVNSKEICRTFDIKDPKTLRDI
                     IHGMVMKGYLIGSSRRKGGGYYLIDNDDERAEAISSLESQVRKEQKRINILKNGNLNE
                     FVEIANEVRNGWNQN"
     gene            complement(4242..5024)
                     /locus_tag="ACGEUI_001583"
     CDS             complement(4242..5024)
                     /locus_tag="ACGEUI_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8166404.1"
                     /translation="MILGDEDALAKIAISYQQNTKKEDAVCEKHGCRYITVLKTGLTV
                     CPDCHREELDNQNALHVQKQYERELENKRLYYLKKLSIMDDELENASFDNFRADTAKH
                     KEMLYWAKTMANDWFSGGKGNIIMTGKAGRGKSHLAYSIIRGLSDKTKKLGLLVNVTD
                     LLSEIKRDFSKEAFWMDKLKDVDYLVLDDLGAEKVSDWSTSIIYSLLNKRTNTIITTN
                     LTPAEIRKIYGERIASRIRKGCDKSHIMEFDGMEDERMKLWN"
     gene            complement(5011..5841)
                     /locus_tag="ACGEUI_001584"
     CDS             complement(5011..5841)
                     /locus_tag="ACGEUI_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFF8166405.1"
                     /translation="MADNKKYYYLKLKENFFESDEAIILESMPDGYIYSNILLKLYLR
                     SLKNDGLLMFNNLIPYNAQMLATITRHQIGTVEKAIQIFRDLQLIEILDNGAIYMTNI
                     QNFVGKSSTDADRKRIEYAKAKQLGQISMKCAEKSPPEIEIELDKERELDKKIDINID
                     KELEVEEEIKDSSSAANVNNLKIISDYFQQEIGILSPNQFEQLSDYITITKMEVDVVK
                     EAITRAADNSKRSFGYVNSILRNWRQNGILTMVKVEEEQRQFQSRKSKQEEVSEYDTW
                     "
     gene            complement(5855..6043)
                     /locus_tag="ACGEUI_001585"
                     /pseudo
     CDS             complement(5855..6043)
                     /locus_tag="ACGEUI_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            6277..6516
                     /locus_tag="ACGEUI_001586"
     CDS             6277..6516
                     /locus_tag="ACGEUI_001586"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166406.1"
                     /translation="MQNEADDYYGLSMPNYYMPLDYQWESSPFKSDTEVYPSSRENFS
                     YYLDEKPVKIKITTDKRIHQFRKYQLFFPHFDNNS"
     gene            6647..6856
                     /locus_tag="ACGEUI_001587"
     CDS             6647..6856
                     /locus_tag="ACGEUI_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166407.1"
                     /translation="MGPNYFHVQFKLGEKVSYNTPSAEGREVIPIKGAEVTKMIFADG
                     NELLSVIHNETVDVYASFPIVLEYH"
     gene            complement(6966..7166)
                     /locus_tag="ACGEUI_001588"
     CDS             complement(6966..7166)
                     /locus_tag="ACGEUI_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8166408.1"
                     /translation="MTRFAIQRLREEKKMSQEELANKSGVSRTTISLIETDKSTTVKL
                     STLQKLAVALDVPIGYFFKHNV"
     gene            7240..7626
                     /locus_tag="ACGEUI_001589"
     CDS             7240..7626
                     /locus_tag="ACGEUI_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166409.1"
                     /translation="MFDYFKLYIIVLETFVETKPDDAYSLFDNLSLNSEFVKFSKDLD
                     KNIVISGTLEVIDNLLDDCLIKGKRRPTKDITFYFFDGVTTTGYLYLQSLKDNNFSSR
                     LKNVLKEEGIPLTPTSITRTIAKLTL"
     gene            complement(7615..>7823)
                     /locus_tag="ACGEUI_001590"
     CDS             complement(7615..>7823)
                     /locus_tag="ACGEUI_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166410.1"
                     /translation="RPKKYPYSQKNYPAPKLHNVIETDNQFLIDDKRIHYVIEDSVKT
                     KTLGDGYVEVTLSIIAKSFTKSQG"
BASE COUNT         2041 a         1595 c         1279 g         2908 t
ORIGIN      
        1 tgcgtaagtt ccgcaattcg ctttgtctgt ctagctctat cttcgctagc acgtttaagc
       61 tgcttttgtg tcctgcttag ctgtgtacgt agtcctgtga tttgcgactc gtaatattgt
      121 cgtgcgtcgc gatagctaaa atacgacacg gttaccatca tcccaaatat tgcgattgca
      181 agaaacaata gtgctttcca atcgtttttt aggacattca ttattttatt aaagttatca
      241 cgtaaatttt gcaacgcttc atctatcgtc attcttccac ctctgccaaa atataagtca
      301 aatctcttcc gtaaggagaa agttgtacga cttgataacc aacaactgtc acttttgcgt
      361 acttgttttt gtcgataaaa tcgttcaggt ggtctgttgc acttttccat tcgtccttaa
      421 cttcaatata tctcttcatt cttccacgct ttcgattaac caatattcaa tgagacgctt
      481 tgtatattcg agtgcttttt ctaaatcctg agtcgcattg cctttgtatc tatgtcgttt
      541 aatgtattta tcaatgtgac tttccatgat tgcgacaaac tggtcccatg ggtacgtctt
      601 ataccacgac tcaatcaaat caatttctcc gtgattataa tgctttggtt tatgtactaa
      661 ttcttctatc atcactccac ctctctcaaa taatttacta tcgttttaca cacgctctca
      721 tttggcaata ttctgcgttc taagagcgct tttaattgcc aagtataaat gcctatctta
      781 tctgctaaaa cgtcatcaga catctttatt ttgcatctgt gagctattaa cagctctgat
      841 atatcgtaag gcaacagatt atcgtaagat ctaggtgtat atttaatatc gttatgccac
      901 tgtctgtgtc ttttcataga cccattctcc gagctctttc tagtgcgtcc gtgcgtttga
      961 tttttttaac gagcttaacg tcaccgtagt ttttaaacat ccacttttcg taaatcttgt
     1021 catcctcatc tgtctttttt tgtttaaggc ggtaagactg cttgattaac attatcattt
     1081 cctttgtcgt gtaaattcgt tggaaccact ccaatacatc aggtggcggt aatctgttta
     1141 gttttttata gtatttgaca gatctataga ctctgtcagc ttcttctttg tctgcgatgg
     1201 taatgttatc gtctaaaaac gctttaattg acggctccat ttgtttgtaa aaatcatcta
     1261 ctagtgtcat aattcgttta tttttacctc gattctggga tttgggctat acagcttacg
     1321 tgtagtgtgc tcgacaatga tgttgtcatc tgtccacaca acctctgatt ttgatatgct
     1381 gtcatatact gctttttcaa ggttgtccaa atcgggcttc tttggcacat ataagagctc
     1441 atttatataa tcctgatact tttgtttagt cttatctttg gcacgctctg acggcttttt
     1501 agacaccaat tctggtgctt ttagataaaa agtaacatca acttttaaac cgtcgtcaaa
     1561 ataaggccct tcgtaattgt ttttaacata gtctgtgacc tgcttccgcc atgccatcat
     1621 atctccatcc tcgtaagctc cactccatct gctaaagcgt ggacgttttt gcggttttgg
     1681 ctcaatcggt attataaact taaccactac tcctccaatt cgtcttcgta tcctataaac
     1741 tctgcgtgtc tgccatctgg tctcctcttt ttatgagctg gcgacgctat aaaaactatt
     1801 gtgtcagcgg taacaccaag tctttctgct agctcatgct ttgtgccaac atctacaaaa
     1861 gagtctccgt cgtagaccgc atatatcctt tgtctatatc tgttagccat atcatttaaa
     1921 acggcagatc atcgtctgaa atatccattg ggtttgaatt gccaaacggt tgactgttat
     1981 cgttttgcga agagttatct tgaccagatt gttgctggct atttcgactt tccaacattt
     2041 ggaaactctc tgcaacaact tccgtcacat agacacgttg ttcttgttgg ttttcgtagt
     2101 tacgtgtctg aatacgaccc gtaactccga tcaaagcacc ttttttagcc cagttggcta
     2161 aattttcagc agactgtcgc cagataacac agttaatgaa atctgcttct ctctccccgt
     2221 tttgctcttt aaatctgcgg tttaccgcaa gtgtgaacgt agctacagct acttgactcg
     2281 ctgtatagcg aagctctgcg tccttggtca tgcgaccaac tagcacaatg ttgttaatca
     2341 tttttttgcc ctgctctctt ttttaattta ttaatcaaat catcagtcga tacaatctgt
     2401 tctgtgcgta aatcttccag tgatttaact tttaaggtat ctgttagcca ttttgttagc
     2461 tctttgacat tttgatttgt ggcttttgca atatcgctta aatcagattt ataagtctct
     2521 acttggatat tgctgatttt cggggtttgg aagcttgttc cttttgacgt tttatttgtt
     2581 ggtttttgcg attgatttgt tttgttgggt ttgctagctt cattgccgtc atcatcttga
     2641 tcgcttgtta tcccaaaaat tgctgatagt gcgtagcgtt ttgcgtaagt gataaccgaa
     2701 ccagcgcctt gtacatcatt ttttgttggt ttaacactta aaggtccgta ttccacccat
     2761 tcgccactcg tgtgcatgac tagcgttgcg acatcgatat aaccgttttc tgcgtttgtt
     2821 gttggatcct gcgaaaaaga tatcccgtta ttagcaaagg cttttgtaat cgcttccgtc
     2881 acgttttcta gaggcacgta tttgcttttg aaaaaggggt tgtctttatc ttttagcggt
     2941 tgctttacct ctagctgagc tttacaaaag gctttagcat attctgttat actttctgat
     3001 ttcctcattt actttacctg caaactttct gtttcgatta gttcaactcc agatatatca
     3061 attccagatt tcaaagcttt cgagatctca gatttcattg gtttgtattc aattttttct
     3121 tgcatgtaat caagaggaat ttttgtttcg tccaaaatct ctactttttt gctttttcgc
     3181 aaagacacct taaacattcc agcgtcaact tttttctttt ggctcaattc cattgcacgc
     3241 ctgattgtct ctttgtattt ttccactttt gcttctgctt gcttttgctt tttgtaaaaa
     3301 gcttcttttt cggctttata catttcgacg tcagcttgag cattttttaa cattttgaca
     3361 aaatactcaa tgttattttc taagtctgat tgaaaatcaa tgctgtcaag cgtattttga
     3421 aaggtttcgt cgtctaaatc taaactttcc agataagcgg cgattccctc aagttcatat
     3481 aaataagcca tgttatttcc tctttctgta ttttaatcat ccaaaatgtg ttgtttagtg
     3541 gaccacttgc tgtcaatctt gcgattaacg attagctctg gccatacatc aaattctgtc
     3601 tcgatgtagt ccatcaagtc ttcgtctgtg tagtctttaa aggtttgata agtctgtttt
     3661 agcataggtt cttcgctgcc tctaagacag tcaatcgtaa agataagcgc atctctaaaa
     3721 ttgctatcaa aggttacgag ctcaccgtta attctgattc caaccatttc gcacctcgtt
     3781 agctatttct acgaattcgt tcaagtttcc gttttttaaa atgtttatcc gcttttgttc
     3841 ttttctaact tgactttcaa gtgagctgat agcttctgca cgctcatcat cgttgtcaat
     3901 tagatagtac cctccgcctt ttcgacggct agaacctatc aaatagcctt tcattaccat
     3961 tccgtggata atgtctctca aggttttcgg gtctttgatg tcaaatgttc gacaaatttc
     4021 ctttgaatta accatcatgt tacttcctat cggcaaataa caaaatattc ttttttctaa
     4081 ctcatctagt ggcattacca tattaaatct ctcctaaaac gtgacttttg agcactctct
     4141 ccacgctctc aattcttcaa ttttctttgt tcggacatct tcatctagcg ccatgatttt
     4201 tgccgcatgt tcctcggata gcccgaaaaa tgttgttagt gtcaattcca tagcttcatc
     4261 ctttcgtcct ccatgccatc aaattccatg atatgacttt tgtcgcagcc ttttctgata
     4321 cgggatgcaa ttctctctcc ataaattttt ctaatttcgg caggtgtcag attggtagta
     4381 atgattgtgt ttgtgcgctt gttaagcaag ctgtaaataa tgcttgtgga ccagtcacta
     4441 actttttcgg cacctagatc atctagcacc agataatcga catcttttag cttatccatc
     4501 caaaatgctt ccttgctaaa gtctcgcttt atctctgaca gtaagtcagt gacatttaca
     4561 agcaagccta attttttcgt tttatcagac aatcccctaa tgatgctata agctaaatga
     4621 cttttgcctc gtcctgcttt gccagtcatg atgatgtttc ccttaccacc actaaaccaa
     4681 tcgttagcca ttgtctttgc ccaataaagc atctctttgt gtttagccgt atcagcccta
     4741 aaattgtcaa atgatgcatt ttccaactcg tcgtccataa ttgataattt tttgagatag
     4801 tacagtcgct tgttttcaag ctctctctca tattgctttt ggacgtgcaa agcgttttga
     4861 ttgtctaatt cttctctgtg acaatctgga catactgtca aaccagtctt gaggactgtg
     4921 atgtatctac aaccatgttt ttcacagaca gcatcttctt tcttggtgtt ttgttgataa
     4981 gatatagcga ttttagcaag tgcgtcttcg tcaccaagta tcatattccg atacctcttc
     5041 ttgtttagat tttctagatt ggaattgcct ttgttcttct tcaactttaa ccatcgtaag
     5101 gattccgttt tgtctccaat ttcgcaatat agagttaacg tatccaaaag aacgtttaga
     5161 attgtcagca gctctcgtta tcgcttcctt aacaacatcc acttccattt ttgtgatggt
     5221 gatatagtct gacaactgtt caaactgatt cggagataag atgccaattt cttgttgaaa
     5281 ataatcagat ataattttta agttatttac attagcagca gaagatgaat cttttatctc
     5341 ttcttctact tctaactctt tatctatatt tatatctatc tttttatcta actctctctc
     5401 tttatctaac tctatctcta tctctggtgg agatttctcc gcacatttca tggagatttg
     5461 tccaagttgt ttagcctttg catattcaat tctttttctg tcagcatctg tgcttgattt
     5521 accaacaaaa ttttgaatat ttgtcatata tatcgcaccg ttatcgagaa tttcgattaa
     5581 ctgtaggtct ctaaaaattt gaatagcctt ttcgacagtg ccaatctgat gccttgtaat
     5641 tgtggcaagc atttgtgcgt tgtaaggaat aagattgtta aacattaata agccatcatt
     5701 ttttaaactt cttagatata acttgagcaa aatattgcta tagatatagc cgtcaggcat
     5761 actttctaaa atgattgctt catcactttc aaagaaattt tcctttaatt ttaagtaata
     5821 atactttttg ttatctgcca tttatctccc ttcttcaaaa atagctttca catttcttat
     5881 cttttaacgt ctcaataatt ccatctaaaa catctgatcg gtttttaagc tcattgtact
     5941 ctttgacaga tattgtgata aaatctttgt tatcttttgt agtatcgcta tatccgagca
     6001 ggtaagcgac cgatacatca aaatgatctg ctagtaattg cgctaattgt tgctgtgatt
     6061 gctcttgttc actctatcta ctacaacatg gttaagataa aattatctga ttgttacatt
     6121 tcaagagtag ataaaggtta cgattggatg tatgatttta gtatcagtaa cttatcgaat
     6181 gtttcagtca ttattaaaaa aattgaactt tacaacaaag atggaaaact aataagcgat
     6241 aatggcttca atccctttca aaaatacgaa gctgacatgc agaatgaagc tgatgattat
     6301 tatggattgt caatgccgaa ttattatatg ccattagatt accaatggga gtcatcgcca
     6361 tttaagtcag atactgaagt atatccatct agtcgagaaa atttctctta ctatttagat
     6421 gaaaagccag ttaaaatcaa aatcacaact gataagcgta ttcatcaatt ccgaaaatat
     6481 cagttattct ttccccattt tgacaataat agttaagata gcgatattcg ttaataaaat
     6541 taagataagt gctgccgtta gtagcatttt ttcttttctc cttttattag ttttgttcct
     6601 cctgcgtgct ataataaagc tatcattacg aaaggaggaa aaaagcatgg gtcctaatta
     6661 ttttcatgtc caatttaagt taggcgaaaa agtatcgtac aacacgcctt cagcagaagg
     6721 aagagaagtc atccctatca aaggtgctga agttacgaaa atgattttcg ccgatggtaa
     6781 cgaattgtta agcgttattc acaacgaaac cgttgacgtt tacgctagct tcccaattgt
     6841 tcttgagtat cattaattcg ttatttctaa accagcaatc gcccgctact gcgtttgttg
     6901 gtttttcttt tccgtaaaaa attcgatttg cttgcatagt gtcctttcca gttttgttta
     6961 acacgttaaa cattatgttt aaaaaaatat ccaataggta cgtctaaagc gactgccaat
     7021 ttttgaagag tgcttagctt aaccgtagta gatttatcgg tttcaataag agatatagta
     7081 gttcgtgaga ctccagattt attggctagc tcttcttgag acattttctt ttcttcacgc
     7141 aatctttgaa ttgcaaacct cgtcattccc ccacctcctt tctatctgtt tttagttcct
     7201 ccaatctgct ataatatggg cagaaaggag gtgaatgtga tgtttgacta tttcaaactt
     7261 tatataattg tcttggaaac cttcgtggaa accaaaccag acgacgctta tagcttattt
     7321 gataatctaa gtcttaacag tgaatttgta aaattctcta aagacttaga taaaaacatt
     7381 gtaatatcag gcactttaga agtaattgat aatttacttg atgattgctt gataaaagga
     7441 aaacgtcgcc caacaaaaga tattactttc tattttttcg atggcgtaac aacaactggt
     7501 tacttatatc ttcaaagtct taaagataat aatttttcga gccgtttaaa gaatgtcctt
     7561 aaagaagaag gtatccctct tacaccaact tctatcacaa ggactatcgc caaactaacc
     7621 ttgtgatttt gtaaaactct tggcaattat ggaaagtgtt acttcaacat agccatcgcc
     7681 aagggttttt gtctttacag aatcttcaat aacataatga atccttttat catctattaa
     7741 gaactgatta tcggtttcaa tgacgttatg aagcttcggc gctggataat ttttttggct
     7801 atacggatat tttttggggc gca
//
LOCUS       DAXWIQ010000062         7555 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000062 DAXWIQ010000000
VERSION     DAXWIQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.062"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..820
                     /gene="cdaA"
                     /locus_tag="ACGEUI_001591"
     CDS             <1..820
                     /gene="cdaA"
                     /locus_tag="ACGEUI_001591"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFF8166411.1"
                     /translation="LLSLFADPWLLAVHLLDILIVAYLIYRFIKALTGTKIMSLVQGV
                     IFFLVLRFIAEWIGFTTITYLMNQVITYGVIAGVVIFTPEIRAGLEKFGRSTQVFLQK
                     QYVSSESALVDALIKSVAYMGPRKIGALIAIEQTQTLQEYIATGIPLNADISSQLLIN
                     IFIPNTPLHDGAVIVGQNKIVAACAYLPLSESKAISKEFGTRHRAAIGLSENSDALTI
                     IVSEETGAISVTRKGQFLHDLSTDEFETVLRTYLMSNSNVTLPWYKKILGGKSK"
     gene            817..1773
                     /locus_tag="ACGEUI_001592"
     CDS             817..1773
                     /locus_tag="ACGEUI_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HFF8166412.1"
                     /translation="MKRFLNSRPWLGMVSVFFAILLFLTAASSNHNNSSSQIYSPIET
                     YTHSLKDVPIDMKYDSDKYFISGYSYGAEVYLTSTNRIKLDSEVNNDTRNFKIVADLT
                     HSHPGTVSVNLRVENLPSGVTATVSPDKISVTIGKKESKVFPVRGSVDAKQIANGYEI
                     SKIETGVNKVEVTSDESTIALIDHVVAKLPDDQVLDRNYSSRVTLQAVSADGTILASA
                     IDPAKTNLSVAVKKITKSVPIRVEAVGMMDDSLSDIQYKLSKQTAVISGSREVLEDID
                     EIIAEVNISDVTKNTSKTVSLSSSQVSIEPSVVTVQLTTTKK"
     gene            1827..3182
                     /gene="glmM"
                     /locus_tag="ACGEUI_001593"
     CDS             1827..3182
                     /gene="glmM"
                     /locus_tag="ACGEUI_001593"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFF8166413.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETERPKV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGNDGFKLADDQELEIEALLDAPEDTLPRPSAEGLGTLVDYPEGLRKY
                     EKFLVTTGTDLSGMTVALDTANGAASVSARDVFLDLNAEIAVIGEKPNGLNINDGVGS
                     TRPEQLQELVKETGADLGLAFDGDSDRLIAVDETGEIVDGDRIMFIIGKYLSEKGLLA
                     HNTIVTTVMSNLGFHKALDKQGINKAITAVGDRYVVEEMRSSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLAKVMKETGKSLSELAAEVTIYPQKLVNIRVENSMKERAMEVPAI
                     ANIIAKMEDEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRTEIGCD
                     N"
     gene            3316..3957
                     /locus_tag="ACGEUI_001594"
     CDS             3316..3957
                     /locus_tag="ACGEUI_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166414.1"
                     /translation="MTKQQIEKLADLLPPFAATLYLDMIPEFQEGYASYVYQTPKQDI
                     QKRRIKQLTKNFERLLDTPSFYVMPVTRKTAEQLLADKGLAYHLLSTVAANQVFAAAV
                     NEDQMLFQVLRNGARVAFVILQEKGEFLEMTLGILETVTENEVPAAIVEAVQTYLLAD
                     MQEKLPSLSIMVSQESSKLFYQEQGFVLLAKGCKPGNENDSDVCQLVKYFSKP"
     gene            4020..5150
                     /gene="hemW"
                     /locus_tag="ACGEUI_001595"
     CDS             4020..5150
                     /gene="hemW"
                     /locus_tag="ACGEUI_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFF8166415.1"
                     /translation="MSKKPTSAYVHIPFCTQICYYCDFSKVFIQNQPVDAYLKALIQE
                     FDSYGIRDLKTLYIGGGTPTAITAKQLEYLLNHLERNLNLDDLEEFTIEANPGDLTPE
                     KIAVLQRSAVNRISLGVQTFNNKQLKQIGRSHNEEQIYSTIANLKTAGFHNISIDLIY
                     ALPGQTLDQVKENVAKALALDIPHLSLYSLILEHHTVFMNKMRRGKLNLPTEDLEAEM
                     FEYIISEMEANGFEHYEISNFTKPGFESRHNLMYWDNVEYFGCGAGASGYLNGIRYQN
                     RVPIQHYLKAVEAGNARLNEEVLRKEEMMEEELFLGLRKKTGVSIQRFQEKFGMSFEE
                     RYGNIVRELQNQGLLVKDDAFVRMTKKGLFLGDSVAERFILD"
     gene            5160..5912
                     /locus_tag="ACGEUI_001596"
     CDS             5160..5912
                     /locus_tag="ACGEUI_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFF8166416.1"
                     /translation="MGLSYQEELTLPFELCDVKSDIKLPLLLDYCLMVSGRQSAQLGR
                     SNNNLLVDYKLVWIVTDYEITIHRLPHFQETITIETKALSYNKFFCYRQFYIYDQEGG
                     LLVDILAYFALLNPDTRKVATIPEDLVAPFETDFVKKLHRVPKMPLLEQSIDRDYYVR
                     YFDIDMNGHVNNSKYLDWMYDVLGCEFLKTHQPLKMTLKYVKEVSPGGQITSSYHLDQ
                     LTSYHQITSDGQLNAQAMIEWRAIKQTESETD"
     gene            5912..6676
                     /locus_tag="ACGEUI_001597"
     CDS             5912..6676
                     /locus_tag="ACGEUI_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFF8166417.1"
                     /translation="MPYKGYLIDLDGTIYQGKNRIPAGERFIKRLQERGIPYLLVTNN
                     TTRTPEMVQSMLANQFHIETSIETIYTATMATVDYMNDMNRGKTAYVIGETGLKSAIA
                     AAGYVEELENPAYVVVGLDSQVTYEMLAIATLAIQKGALFIGTNPDLNIPTERGLMPG
                     AGALNALLEAATRVKPVFIGKPNAIIMNKSLEVLGIQRSEAVMVGDNYLTDIMAGIQN
                     DIATILVTTGFTRPEEVPTLPIQPDHVLSSLDEWRL"
     gene            6676..7308
                     /locus_tag="ACGEUI_001598"
     CDS             6676..7308
                     /locus_tag="ACGEUI_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFF8166418.1"
                     /translation="MVENTKLLCSWVWLLALAILITIYSTWLWYPLEVDHLKLEQVVF
                     MSKDAILHNYNGLLNYLTNPFVTRLEFANFHSSADGLKHFADVKWLFHLTQVVFLGLL
                     YPTLKTFTQRLKTKRFWLLQKPLILAALFPLMIGLMASFIGFEHFFTLFHQVLFVGDS
                     SWLFDPLKDSVIWILPEVFFLHCFLFFMIVYEIILWSLVGLARWQRLNVE"
BASE COUNT         2314 a         1327 c         1609 g         2305 t
ORIGIN      
        1 tttattaagc ttatttgcgg atccttggct gctggcagtg catcttttag atattctaat
       61 tgttgcctat ttgatttatc gttttattaa agcattgact ggaactaaaa tcatgtcttt
      121 agttcaaggg gtcattttct ttttggttct tcgttttatt gcagagtgga ttggctttac
      181 gacaatcaca tacttaatga accaagtcat tacttatggt gtaattgcag gagtagtcat
      241 ttttacccct gaaattcggg caggactgga gaaatttggt cgttctacgc aagttttttt
      301 acaaaaacag tacgtcagca gtgaatctgc tttggtcgat gctttgatta aatctgtggc
      361 ctatatgggt ccacgcaaga taggtgcatt gattgctatt gaacagactc aaaccttaca
      421 agaatatatt gcgacaggaa ttccgctgaa tgccgacatt tcaagtcaac tactcatcaa
      481 tatttttatt ccgaatacgc cgcttcatga tggagctgtt atcgttggtc agaataagat
      541 tgtggcagcc tgtgcttact tgcctttgtc agaatcaaag gcaatttcca aagaatttgg
      601 gacccgtcat agagcggcta ttggattatc tgaaaactct gatgcactaa ctatcattgt
      661 ttcagaagaa acaggagcta tttcagtaac tcgcaaaggc caatttttac acgatttaag
      721 cacagatgaa tttgaaacgg tacttaggac ctacctgatg agtaattcaa atgttacttt
      781 accatggtat aagaaaattc taggagggaa atctaaatga aacgattttt aaatagtcgt
      841 ccttggttag gaatggtatc agtctttttt gcgattctcc tttttttaac tgcagcatcg
      901 agcaatcata ataactctag ctcgcaaatc tatagtccga ttgaaaccta tacgcatagc
      961 cttaaagatg taccaattga tatgaagtat gatagtgaca aatattttat cagtgggtat
     1021 tcttatggtg cggaagttta tctaacctcc actaatcgta tcaaattaga ttctgaagtg
     1081 aacaatgata cacgtaattt taagattgtg gcggatctaa cacatagtca tccaggtaca
     1141 gtaagtgtca acctaagagt agaaaacctt ccttctgggg tgacagctac cgtatctcca
     1201 gataaaatat cagtcacaat tggcaaaaaa gaatctaagg tatttcctgt tagaggaagc
     1261 gttgatgcta aacaaattgc aaatggttat gaaattagca aaattgaaac gggggttaat
     1321 aaggttgaag tgaccagtga tgaatctacc attgctttaa ttgatcacgt tgtggcaaag
     1381 ttacctgatg atcaagtgtt agataggaat tatagtagtc gagtgacctt acaagctgtc
     1441 tcagctgatg gaactatttt agcaagtgca attgatcctg caaaaacaaa tttatcagtt
     1501 gctgtaaaaa aaataacgaa atcagtcccg attagagttg aggcagtggg gatgatggat
     1561 gatagcttat cagatattca atacaaattg tcaaaacaaa cagctgttat ctcaggtagt
     1621 cgagaggtct tagaagatat tgatgaaatt attgcagaag ttaacatttc tgatgtcact
     1681 aaaaatacta gtaagaccgt gagtttgtct tctagtcagg tgtcaattga gccgtcagta
     1741 gtgaccgttc agttgacaac caccaaaaaa taatggataa taactaggaa aattgaatgg
     1801 aggaagccat taggcttata taaattatgg gaaaatattt tggaacagat ggtgttcgtg
     1861 gcgaagcaaa tgttgagctg acacctgagt tagcttttaa actgggccga ttcggtggtt
     1921 atgttttaag tcaacatgag accgaaagac ctaaagtttt tgtggcgcgt gacacccgta
     1981 tttcaggaga aatgttggag tcagccttga ttgctggtct actatctgtt ggtattgaag
     2041 tttataaact tggcgtttta gcgacacctg gtgtctctta tttagttaga acagaaaaag
     2101 caagtgcagg tgtgatgatt tctgcaagtc ataaccctgc gcttgacaat ggcatcaaat
     2161 tctttgggaa tgatgggttt aagttagctg atgaccaaga attggaaatt gaagctctct
     2221 tagatgctcc ggaggacacg cttcctcgcc cgagtgcaga aggacttgga accttggtag
     2281 attatccaga aggacttcgt aaatatgaaa agttcttggt aacaactggt actgacttaa
     2341 gcggtatgac agttgcctta gacactgcta atggagctgc ttcagtgtca gcacgtgatg
     2401 tctttttaga tttaaatgca gaaattgctg tcattggtga aaaaccaaat ggtcttaata
     2461 ttaatgacgg tgttggttca acgcgtccag aacaacttca agagttagtt aaggaaacag
     2521 gagcagatct tggtttagcc tttgatggcg atagcgaccg tctgattgct gtggacgaaa
     2581 ctggcgagat tgttgacggt gatcgtatta tgtttatcat tggtaaatat ttgtcagaaa
     2641 aagggcttct tgctcacaat accattgtca caacggtgat gtctaacctc ggttttcata
     2701 aagctttaga taaacaaggt attaataaag caattacagc agttggagac cgctatgtgg
     2761 ttgaagaaat gcgtagctca ggatacaatc tcggtggtga acaatcaggc catgtcatta
     2821 tcatggacta caataccact ggagatggtc agttgacagc tatccaatta gccaaggtga
     2881 tgaaggaaac tggaaaatca ctttcggaat tagctgctga agtaaccatt taccctcaaa
     2941 aactggtgaa tattcgtgtg gaaaacagca tgaaagaacg tgctatggaa gttccagcta
     3001 ttgcgaacat cattgcaaaa atggaggatg aaatggctgg taatggtcgt attctcgtga
     3061 gaccaagtgg tacagaacct cttcttcgtg tcatggcaga ggcgccaaca gatgccgaag
     3121 ttgattatta tgtcgatact attgctgatg tcgtccgtac agaaatcgga tgcgataact
     3181 aacgagttga gtacaaacaa gaaaccttat ttctaaggct tctgtcaaca attggaaaga
     3241 ggcttggggc gtttagccca agtctctttt tataaattgt tataaattgc taaagtagaa
     3301 gagaaaggat attggatgac aaaacagcaa atcgaaaaac tagcggactt actgcctcct
     3361 tttgcagcca cactttactt agacatgatt cctgaatttc aagaaggtta tgcttcttat
     3421 gtttatcaaa caccaaaaca agatattcaa aagcgccgca tcaagcagtt aactaaaaat
     3481 tttgaacgat tactagatac cccatctttt tatgtgatgc ctgtgacaag aaaaacagca
     3541 gagcaattgc tagcagataa aggcctagct tatcatttgt tatcgacagt tgcagctaat
     3601 caagtgtttg cggctgctgt taacgaggat cagatgcttt ttcaagtgct tcgaaatgga
     3661 gcaagagtag cctttgtgat tcttcaagaa aagggggaat ttctagaaat gactttaggg
     3721 attcttgaaa cagtgacaga aaacgaggtt ccagcagcta ttgtggaggc tgtccagaca
     3781 tatcttctgg cagatatgca agagaaactc ccttctttaa gcattatggt cagtcaagaa
     3841 tcaagcaagc ttttttacca agaacaaggg tttgttttgc tagctaaggg ttgcaagcca
     3901 ggaaacgaaa atgattctga cgtttgtcag ctcgtaaaat acttttccaa gccatgaaga
     3961 atcaagggaa atggtttatt catagaaaca ttttatcatc aaagtggtat aataagacca
     4021 tgtcaaaaaa acctacttca gcctatgtgc acattccttt ttgcacacaa atttgttatt
     4081 attgcgattt ttcaaaagtc tttatccaaa atcaacctgt tgatgcttat ttgaaagcct
     4141 tgattcaaga atttgactct tatggcattc gagacctaaa aaccctttat attggaggag
     4201 gtacgcctac agctatcact gctaaacagt tggagtatct cctaaatcac cttgaacgaa
     4261 acctcaattt agatgatctt gaagaattca caattgaggc aaatcctggt gatttgacgc
     4321 cagaaaaaat tgcggtgctt cagaggtcag ctgtcaatcg gatttccttg ggggtgcaga
     4381 cttttaataa caagcaatta aagcagattg gccgtagcca taatgaagaa caaatttata
     4441 gcaccattgc caatttaaaa actgcaggct ttcacaatat ttccattgat ttaatttatg
     4501 ccttgccagg tcaaacctta gatcaagtca aggaaaatgt tgctaaagct ttggcacttg
     4561 atattcccca ccttagtctt tatagcctta ttttggaaca tcacactgtt tttatgaaca
     4621 aaatgcgacg tggtaaactt aacttaccaa cggaagactt ggaagcggaa atgtttgagt
     4681 acattatttc agaaatggaa gctaatggtt ttgagcatta tgaaatttcc aattttacca
     4741 aaccaggttt tgaaagtcgc cataacttga tgtattggga taatgtggag tattttggat
     4801 gtggtgcagg ggcttcaggt taccttaatg gcattcgtta tcagaatcgg gtgcccatcc
     4861 agcattatct taaagcggtt gaagcaggga atgctagact aaatgaagaa gtgctgcgca
     4921 aagaggaaat gatggaagag gaactctttt tgggcttacg taaaaagaca ggagtctcca
     4981 tccagcggtt ccaagaaaaa tttggtatgt cttttgagga gcgttatgga aatattgtta
     5041 gggagcttca aaaccaagga ctgcttgtca aagatgacgc ctttgtcaga atgactaaaa
     5101 agggtctttt cttaggtgat tctgttgctg agcggtttat attagattag gagagtatta
     5161 tgggattaag ttatcaggag gagttgacac ttccttttga attatgtgat gtcaaatcag
     5221 atataaaatt gcccctttta ttagactatt gtttgatggt ttctggtaga cagtctgcgc
     5281 aattaggacg aagtaacaac aatcttttag ttgattacaa gcttgtttgg attgtaacgg
     5341 attatgagat cactattcat cgcttgccac attttcaaga aaccatcacc attgaaacaa
     5401 aagccctttc ctataataaa tttttttgtt atcgccaatt ttatatttat gatcaagagg
     5461 ggggtctttt agtggatatc ttagcctatt ttgctttgtt aaacccagat acgcgaaaag
     5521 tggcaactat tccagaagat ttagtagcgc catttgagac tgattttgtt aaaaagctac
     5581 accgtgttcc taaaatgcct cttttagaac aatcaattga tcgtgattat tatgtgcgtt
     5641 attttgatat tgatatgaat ggtcatgtca acaacagtaa atatttagat tggatgtatg
     5701 atgtgttggg gtgtgagttt ttaaaaacgc atcagcctct taagatgact ttgaaatatg
     5761 ttaaagaagt ctcaccaggc ggtcaaatta cttccagtta ccatttggac caattaactt
     5821 cttaccatca aatcacctca gatgggcagc tgaatgccca agccatgatt gaatggcgag
     5881 cgattaaaca aacagaaagc gagacagact agtgccttat aagggttatt taattgattt
     5941 agacggaacg atttaccaag ggaaaaaccg tattccagca ggggagcggt ttatcaaacg
     6001 cctacaagaa agaggcattc cctatttatt agtgactaat aataccacaa gaacacccga
     6061 aatggtgcaa agcatgctgg ctaatcagtt tcatattgag acaagcattg agaccattta
     6121 tacggctacc atggcaaccg tcgattacat gaatgacatg aaccgtggca agacagctta
     6181 cgttattgga gaaacagggc tcaaatcagc tattgcagca gcaggttacg tagaagaact
     6241 tgagaatccc gcttacgtgg tggttggttt agacagtcag gtgacctatg agatgttggc
     6301 tatagccacc ttggctattc aaaaaggtgc cctctttatt gggacaaatc ctgatttaaa
     6361 cattccaacc gaacgtggtt tgatgccagg agcaggtgca ttgaatgcct tactagaagc
     6421 tgccacacgt gtaaaacctg tttttattgg caagcccaac gctatcatta tgaataaatc
     6481 actagaggta ttgggcattc agcgctctga ggctgtcatg gttggcgata attacttgac
     6541 tgatatcatg gcaggtattc aaaatgatat tgcaacgatc ctcgtgacaa caggctttac
     6601 cagacctgaa gaggtaccaa ccttaccaat tcagccagat catgtcctat ccagccttga
     6661 tgagtggagg ttgtgatggt agaaaacact aaattactat gtagctgggt atggttatta
     6721 gcgttagcta ttctcataac gatctattca acgtggctgt ggtatcctct tgaggtggac
     6781 catctaaagt tagaacaagt tgtttttatg agcaaggatg ctatcttgca taactataac
     6841 ggactactca attacctcac caatcctttt gtgacaaggt tggaatttgc taattttcac
     6901 tcatcggcag acggactcaa gcattttgca gatgtgaagt ggctttttca cctaactcaa
     6961 gtcgtttttc ttggccttct ttacccaacc cttaagactt tcacacaaag gttaaaaacc
     7021 aaacgtttct ggctccttca aaaaccttta atactagcag ctttgtttcc tcttatgatt
     7081 ggtctaatgg ctagtttcat tgggtttgaa cattttttta ctctttttca tcaggtgctt
     7141 tttgtaggag atagcagctg gctctttgat cccttgaaag attctgtgat ttggatcctt
     7201 ccagaagtct ttttcttgca ctgtttcctt ttctttatga tagtttacga aatcatcctg
     7261 tggagcttag taggtttagc aagatggcag cgcctaaatg tagaatgata aaaggattaa
     7321 gagattaatt tccctaaaaa tgataaaaca agcgttttga aagcgcttgt ttttttggtt
     7381 tgcagtcaga gtagaataga agtatcaaaa aaagcaccga ctcggtgcca ctttttcaag
     7441 ttgataacgg actagcctta ttttaacttg ctatgctgtt ttgaatggtt ccaacaagat
     7501 tattttataa cttttataac aaataatcaa ggagaaattc aaagaaattt atcag
//
LOCUS       DAXWIQ010000063         7249 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000063 DAXWIQ010000000
VERSION     DAXWIQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.063"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(94..366)
                     /locus_tag="ACGEUI_001599"
     CDS             complement(94..366)
                     /locus_tag="ACGEUI_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFF8166419.1"
                     /translation="MSDAFTDVAKMKKIKEDIRAHEGQLVELTLENGRKREKNKIGRL
                     IEVYSSLFIIEYSDSSDTPGAIDNSYVESYTYSDILTEKTLIRYLD"
     gene            complement(383..1744)
                     /gene="dnaB"
                     /locus_tag="ACGEUI_001600"
     CDS             complement(383..1744)
                     /gene="dnaB"
                     /locus_tag="ACGEUI_001600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFF8166420.1"
                     /translation="MPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDDFY
                     KYAHKIIFRAMITLSDRNDAIDATTIRTILDDQDDLQSIGGLSYIVELVNSVPTSANA
                     EYYAKIVAEKAMLRDIIARLTESVNLAYDEILKPEEVIAGVERALIELNEHSNRSGFR
                     KISDVLKVNYEALEARSKQTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGKTA
                     FVLNIAQNVGTKQKKTVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNV
                     TIAQGALAEAPIYIDDTPGIKITEIRARSRKLSQEVDGGLGLIVIDYLQLITGTKPEN
                     RQQEVSDISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADI
                     VAFLYRDDYYRKECDDAEEAVEDNTIEVILEKNRAGARGTVKLMFQKEYNKFSSIAQF
                     EER"
     gene            complement(1780..2232)
                     /gene="rplI"
                     /locus_tag="ACGEUI_001601"
     CDS             complement(1780..2232)
                     /gene="rplI"
                     /locus_tag="ACGEUI_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFF8166421.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATSQSI
                     GELKGKQKAEEKAQAEILAEAQAVKAVLDEDKTRVQFQEKVGPDGRTFGSITAKKISE
                     ELQKQFGVKVDKRHIVLDHPIRAIGLIEVPVKLHKEVTAEIKLAITEA"
     gene            complement(2229..4205)
                     /locus_tag="ACGEUI_001602"
     CDS             complement(2229..4205)
                     /locus_tag="ACGEUI_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFF8166422.1"
                     /translation="MKKFRFETIHLIMMGLILFGLLALCVSIMQSKILILLAIFLVLL
                     FVVALLWYQKEAYQLSDLAHIELLNEQTEDNLKTLLDNMPVGVVQFDQETNAVEWYNP
                     YAELIFTTEEGFIQNGLIQQIITEKRREDISQTFEVSGNKYTSYIDVSSGIFYFFDSF
                     VGNRQLADASMLRPVVGIISVDNYDDITDDLSDADTSKINSFVANFIDEFMESKRIFY
                     RRVNMDRYYFFTDFKTLNDLMDNKFSVLEEFRKEAQDAQRPLTLSIGISFGEENHSQI
                     GQVALENLNIALVRGGDQIVIRENADHTNPIYFGGGSVSTVKRSRTRTRAMMTAISDR
                     IKMVDNVFIVGHRKLDMDALGSAVGMQFFAGNIIENSFAVYNPDEMSPDIERAIERLQ
                     ADGKTRLISVSQAMGLVTPRSLLVMVDHSKISLTLSKEFYEQFQNVIVVDHHRRDDDF
                     PDNAILTFIESGASSAAELVTELIQFQNAKKCLNKIQASVLMAGIMLDTKNFSTRVTS
                     RTFDVASYLRSKGSDSVEIQNISATDFEEYKQINEIILQGERLGDSIIVAAGEKNHLY
                     SNVIASKAADTILSMAHVEASFVLVETASHKIAISARSRSKINVQRVMEKLGGGGHFN
                     LAACQLTDISLPQAKYLLLKTINMTMKETGEVES"
     gene            complement(4296..6194)
                     /gene="mnmG"
                     /locus_tag="ACGEUI_001603"
     CDS             complement(4296..6194)
                     /gene="mnmG"
                     /locus_tag="ACGEUI_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFF8166423.1"
                     /translation="MTHEFTESYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KSLYAREMKHTVEKQANLTLRQTMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTA
                     LRGEIILGELKYSSGPNNSLASVTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTE
                     IQPGDDKPNHFSFMSKDADYLKDQIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRDTEEVYVQGLSTSLPEDVQKDLIHSIKGL
                     EKAEMMRTGYAIEYDIVLPHQLRATLETKLISGLFTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGRDIGLVDDERWKAFEIKKNQFDNELKRLNSIKLKPIKETNDRVQDLGFKPLTD
                     AMTAKEFMRRPEIDYATAVSFVGPAAEDLDAKIIELLETEIKYEGYIRKALDQVAKMK
                     RMEEKRIPTNIDWDAIDSIATEARQKFKKINPETIGQASRISGVNPADISILMIYLEG
                     NGKAHRKY"
     gene            complement(6318..6635)
                     /locus_tag="ACGEUI_001604"
     CDS             complement(6318..6635)
                     /locus_tag="ACGEUI_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFF8166424.1"
                     /translation="MIVRNGKNFLTRDADDQYYTIGGTSLVGEKTHETVLRETLEEVG
                     IRAKVNQLAFMVENHFDIDDVFWHNIEFHYLVSPLEDPKLEMIENASDRFVNGFQSIN
                     WIK"
BASE COUNT         2240 a         1501 c         1114 g         2394 t
ORIGIN      
        1 ttttcaaaat aaaaagaata caggcagaat aatcttctat cgctaataat tatatcaatt
       61 tattgtaaag aaaaagcctc tatgaggcaa aaatcaatct aaataacgaa ttaatgtttt
      121 ttctgtcaaa atatctgaat aggtgtatga ttcgacataa gaattatcaa ttgctcctgg
      181 agtatcagag ctatcactat actcaatgat aaagagtgaa ctgtaaactt caataagcct
      241 accaatttta tttttttccc gtttccgtcc attctcaagg gttaattcta ctaactgtcc
      301 ttcatgggct cttatgtcct ctttaatttt tttcattttt gcaacatctg taaatgcatc
      361 gctcattttt tgtctctctt tcttatcttt cttcaaactg ggctatactt gagaatttgt
      421 tgtattcttt ttggaacatc agtttgactg ttccacgcgc cccagctcta tttttctcga
      481 ggataacttc aattgtgtta tcttcaacag cttcttcagc atcatcacat tctttacggt
      541 aataatcgtc ccggtataag aaggctacaa tatcggcatc ctgctcaata gatcctgatt
      601 cacgaatatc tgataaaact ggtcgtttat cttgcctttg ctcaacgcca cgagaaagct
      661 gacttagggc aataactggt actttcaatt ctttagctag gattttaagc tgtcttgaaa
      721 tatctgaaac ctcttgctga cgattttcgg gtttagttcc tgtaatcaac tgtaagtagt
      781 caattacaat gagacctaaa ccaccatcca cttcttgaga caatttccgt gatcttgcgc
      841 ggatttcagt aattttaatc ccgggcgtat cgtcaatata aatcggtgct tctgccaaag
      901 ctccctgagc aattgttaca ttattccaat cctgatctgt gagttgccct gttcttaaac
      961 tgtgcgaatc aaccattcct tctgctgcaa gcatacgatc tactaaactt tcagcaccca
     1021 tttccaaaga aaaaatagca acagtctttt tttgcttagt ccccacattt tgcgcaatat
     1081 taagaacaaa ggcagtcttc cccactgctg gccgagcagc taaaataact aattgatctg
     1141 ggtgtaaacc tgttgtaatc ttgtcaaggt ctctaaaacc agttggtaaa cctgtaacat
     1201 ttgaagtctg cttagaacgt gcttctaaag cctcgtaatt aacttttagc acatctgaaa
     1261 ttttgcgaaa cccactacga ttactatgtt cattgagttc aattaaagct ctctcaactc
     1321 cagcgataac ctcttctggt tttaaaattt catcataagc taggttgaca gattctgtca
     1381 acctagcaat aatatcacgc aacatagctt tctcagctac aattttagca taatattctg
     1441 cattagcact agttgggaca ctattaacta gttcaacaat ataggataag ccaccaatac
     1501 tttgcagatc atcttgatca tctaggattg ttcttatagt ggttgcatca atggcatcat
     1561 tacgatcgct gagggtaatc attgcccgaa agataatttt atgagcgtac ttataaaaat
     1621 cgtctggact gataaattct ctcactgcaa tcagcttatc aggtgagata aagattgacc
     1681 caagaacaga ttgctctgct agtaaatctt ggggttgaac tcgtaattca gctacttcag
     1741 gcaacttcat aacctccaaa tcttgtgatg ttttttatat tatgcttctg tgatagccaa
     1801 tttaatctca gctgttactt ccttatgtaa tttcacagga acttcaatta gaccaatggc
     1861 gcggatagga tgatctaaaa caatatgacg tttatcaact tttacaccaa attgcttttg
     1921 caattcttct gaaatctttt tagctgtgat ggaaccaaat gtacgtccat ctggaccaac
     1981 tttttcttga aattgaacac gtgttttgtc ctcatccaaa acagccttga cagcttgagc
     2041 ttctgctaat atttcagctt gagctttttc ttcagctttt tgttttcctt ttaattcacc
     2101 gatgctttga ctagttgctt ctttagctaa atttttctta ataaggaaat tttgagcata
     2161 gcctgttggg acctctttga tttctccttt tttacctttt ccttttacat ctgctaaaaa
     2221 aataactttc atgattctac ttctcctgtt tctttcattg tcatattaat agttttcaat
     2281 aataagtact tcgcttgggg aagactgata tccgttaact gacaggcagc aagattaaag
     2341 tggcctcctc cacctaattt ttccatcaca cgttgaacat tgattttact acgacttcta
     2401 gctgaaatag caatcttatg agaagctgtt tcaactaata caaagctagc ctcaacgtga
     2461 gccattgaca gtatcgtatc tgcagcttta ctagcaataa cattgctata aagatgattt
     2521 ttttctcctg cagctacaat gatactgtca ccaagacgtt ctccttgtaa aataatttca
     2581 tttatttgct tatattcttc aaaatctgtt gcggatatat tttgaatttc aacactatca
     2641 cttcctttgc ttcttaagta actagcgaca tcaaaggtac gacttgtcac tcgcgtagaa
     2701 aaatttttag tatcaagcat aatacctgcc attaaaacac tggcttggat cttatttaaa
     2761 cattttttag cattttgaaa ttgaattaac tcagtgacga gctccgcagc actacttgct
     2821 ccactttcaa taaaagttaa gatagcatta tcaggaaaat catcatctct tctgtgatga
     2881 tcaactacaa taacattttg aaactgttca taaaattctt tagaaagtgt taaagatatc
     2941 ttagaatggt caaccattac taaaagtgat cttggagtca ctaaacccat tgcttgagaa
     3001 acactgatta gacgagtctt tccgtcagcc tgcaatcgct caatagccct ttcaatatct
     3061 ggactcatct catctggatt gtaaacggca aaactatttt caataatgtt acctgcgaaa
     3121 aattgcatac caacagctga acctagtgca tccatatcaa gtttcctatg tccaacaata
     3181 aaaacattgt ccaccatctt gattcgatct gaaatagctg tcatcatagc acgggtacgt
     3241 gttcttgaac gtttaactgt agaaacagac cctcccccga aataaattgg attcgtatga
     3301 tccgcatttt cacgaatgac aatctgatca ccaccacgaa caagcgcaat attaagattc
     3361 tcaagagcta cctgtccaat ctggctatga ttttcttcac caaaagaaat tcctatgctg
     3421 agtgttaaag ggcgctgagc atcttgagct tctttacgaa actcttctaa gacagaaaat
     3481 ttattatcca tcaaatcatt taatgtttta aagtctgtaa agaaatagta gcgatccata
     3541 ttaacacgac ggtaaaaaat ccgttttgac tccataaact catcaatgaa gttagctaca
     3601 aatgaattaa tttttgaggt atctgcatct gataaatcat cagtgatatc atcataatta
     3661 tcaaccgaaa taatgccaac aaccggtctt aacatactag catcagctaa ttgcctgttt
     3721 cccacaaagc tatcaaaaaa ataaaaaatt cctgatgata catcaatata agaagtgtat
     3781 ttattaccag aaacttcaaa tgtttgagaa atatcttcac gacgtttttc cgtaataatt
     3841 tgctgaatca aaccgttttg tataaaaccc tcttcagttg taaaaattaa ttctgcataa
     3901 ggattatacc attctacagc gttggtctct tgatcaaatt gaactactcc aacaggcata
     3961 ttatcaagta gcgtttttag gttatcttct gtttgttcat ttaagagctc aatatgagct
     4021 aagtctgata attgataagc ttctttttga taccataaaa gagcaacaac aaatagcaaa
     4081 acgagaaaaa tagctaataa tattaatatt tttgattgca taatactgac acaaagtgcc
     4141 agcagtccaa ataaaatcaa tcccatcata atcaaatgaa tggtttcaaa acgaaatttt
     4201 ttcatcttta taacctctta agcacacatt ataccataaa aaagccctga aatacagagc
     4261 ttccacttgc tttgagatta caatttcatt tccatttaat attttcgatg agcttttccg
     4321 tttccttcta aataaatcat caagatactg atgtccgcag gattgactcc tgaaatacga
     4381 ctagcttgtc caattgtttc aggattaatt ttcttaaatt tctgacgcgc ctcagttgca
     4441 atagaatcaa tagcatccca atcaatattt gtaggaatac gtttttcttc catgcgtttc
     4501 atcttagcaa cctgatctaa agctttgcga atatagcctt cgtacttgat ttctgtttct
     4561 aaaagttcaa tgattttagc atctaaatct tcagccgcag gaccaacaaa agagactgca
     4621 gtcgcataat caatttctgg acgacgcatg aattctttag cagtcatcgc atcggttaac
     4681 ggtttgaagc ctagatcttg aacgcggtca ttggtttctt taatcggttt taatttgata
     4741 ctgttcagac gttttaattc gttatcaaat tggtttttct taatttcgaa ggccttccaa
     4801 cgctcatcat caaccaaccc aatgtcgcga ccaatctctg tcaaacgcat atcagcgttg
     4861 tcatgacgta aaatcaaacg atactcagct cgcgatgtca acaagcgata aggttccaag
     4921 gttcctttgg taaccaaatc gtcaatcata acgccaatat aggcatcgct ccgtttcaaa
     4981 ataagttctg gcttaccttg aactttcaaa gcagcattga ttccagcaat aagtccttgg
     5041 ccagcagcct cttcataccc agaagttcca ttagtttgcc cagccgtaaa gagtcctgaa
     5101 attagcttgg tttcaagtgt ggcacgtagc tggtgaggta aaacaatatc atattcaata
     5161 gcataaccag tgcgcatcat ttcagccttt tcaagtcctt tgatcgaatg aatcaaatct
     5221 ttttggacat cttctggaag actcgttgac aacccttgaa catacacttc ttctgtatca
     5281 cgcccctctg gctctaagaa aagttgatga cgttctttat cagcaaagcg aacaatctta
     5341 tcttcaatag atgggcagta acgagggcct acacctttaa caatgccaga aaacatcggt
     5401 gctctgtaaa ggttctgatt aatgatatca tgactagttt gattcgtata agtcaaccag
     5461 cagggaattt gatctttgag ataatcagca tcttttgaca taaatgaaaa atgattaggt
     5521 ttgtcatcac ctggctgaat ttccgtttga tcgtaattaa tagaagatgc tttgacacga
     5581 ggaggcgttc ctgttttgaa tctgccaatt tctaagccga gtttttttaa attatctgct
     5641 aaggttactg atgccaaact attattaggc ccagaagaat atttgagttc tcctaaaata
     5701 atttcgccac gcagagctgt tcctgttgtc actacaactg ccttagctgc aaatttttgt
     5761 cctgtggctg ttaaaacacc tacaactcgt ccatcttcaa ctagaatatc atcaatcatc
     5821 gtttggcgaa gagtcaaatt agcctgtttt tcaaccgtat gtttcatttc acgcgcatac
     5881 agggatttat ctgcttgagc tcggagagcg cgtacagctg gtcccttacc tgtattaagc
     5941 attttcatct ggatataagt tttatcaata tttttaccca tttcgccgcc aagagcatca
     6001 atctctctaa ccactatccc tttggcagat cctccaatag aaggattaca cggcataaaa
     6061 gccaacatat caaggttgat tgttgccaat aaagttttac aacccatacg actagtggcc
     6121 aaactggctt caacaccagc atgacctgca cctatgacaa tgacgtcata gctttctgta
     6181 aattcatgtg tcattttttt aaaatccttt atttcagtct attcatttaa aatcatacgc
     6241 tttagtttag ccttcaatac ggacaatatg tcctggccat ttagccaact cggtttgtaa
     6301 aaactcagga acaagattca tttgatccag ttgattgatt ggaatccatt cacaaacctg
     6361 tcgcttgcat tctctatcat ttctagttta gggtcttcta aggggcttac caaataatga
     6421 aattcaatat tatgccagaa aacatcatcg atatcaaaat gattctcaac cataaaagcc
     6481 agttgattaa ccttagctcg aataccaact tcctctaagg tttcacgtaa aacagtttca
     6541 tgcgtttttt cacctaccaa acttgtacca ccaatggtgt aatattggtc atctgcatct
     6601 ctagttaaaa aattcttacc atttctaaca atcaagcact agctcgtaaa ctaaagttat
     6661 ctgacctcta gtgattctaa attccagtcc tattagccat ctcatttcta gagctaaacc
     6721 ataaaaaggg ccaaaaccct agaagattgc aggacaaaaa aacctacaac tttcatgagc
     6781 tttgaccatc attccggttg ttactatcta ttttaaaaaa aatgattagt gattgtcaat
     6841 attgatttca tcacagacct ttacagtaat gtcaatgttt ttacaactct gtcaatctat
     6901 tgtaatgttt ttaaaatgat tacaaaagta tcactatgtc taatctagcg aggcgagaaa
     6961 taaaaatgtc atcagtatta tcatacgatt ttttgatatc ttctaaagta gtaaaataca
     7021 gatactgttg tttctaagtc tacaagtaag aacgccgata acaaaaccat aaaagtataa
     7081 ctaggaattt ccaataattt tttacacatt tagtattaga taataggtac taataatcat
     7141 gaaataatct gttttacgaa atacctaatc atcaaaatat ctgttgtaat actagagaaa
     7201 gtgagtctca tgatcgtatg atttctacag taactaataa agtaatgac
//
LOCUS       DAXWIQ010000064         7232 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000064 DAXWIQ010000000
VERSION     DAXWIQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.064"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            591..674
                     /locus_tag="ACGEUI_001605"
     CDS             591..674
                     /locus_tag="ACGEUI_001605"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFF8166425.1"
                     /translation="MDYCNNKRIKIKLKGLSPLQYKTKSFT"
     gene            complement(712..1635)
                     /locus_tag="ACGEUI_001606"
     CDS             complement(712..1635)
                     /locus_tag="ACGEUI_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8166426.1"
                     /translation="MSEKLVEVKDLEISFGEGKKKFVAVKNANFFIKKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSSGQILYDGKVINGRKSKSEANELIRKIQMIFQDPAASLNE
                     RATVDYIISEGLYNFNLFKTEEERKEKIKNMMAEVGLLSEHLTRYPHEFSGGQRQRIG
                     IARALVMNPEFVIADEPISALDVSVRAQVLNLLKRMQAEKGLTYLFIAHDLSVVRFIS
                     DRIAVIHKGVIVEVAETEELFNNPIHPYTQSLLSAVPIPDPILERQKELVVYHPDQHD
                     YTLDKPSMVEIKPNHFVWANQAEIEKYQKEL"
     gene            complement(1628..2698)
                     /locus_tag="ACGEUI_001607"
     CDS             complement(1628..2698)
                     /locus_tag="ACGEUI_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8166427.1"
                     /translation="MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFV
                     GESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQELTDLKTNKEWAKIRGSKIATIFQ
                     DPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
                     SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITH
                     DLGVVASIADKVAVMYAGEIVEFGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAI
                     PGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAKTWLLHPEAPKVQKP
                     EVIQDLHQKILRKMSQQEEGNV"
     gene            complement(2707..3633)
                     /gene="oppC"
                     /locus_tag="ACGEUI_001608"
     CDS             complement(2707..3633)
                     /gene="oppC"
                     /locus_tag="ACGEUI_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009569326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFF8166428.1"
                     /translation="MESIDKSKFRFVERDSEASEVIDTPAYSYWKSVFRQFFSKKSTV
                     FMLVILVTVLMMSFIYPMFANYDFNDVSNINDFSKRYIWPNAEYWFGTDKNGQSLFDG
                     VWYGARNSILISVIATLINITIGVVLGAIWGVSKAFDKVMIEIYNIISNIPSMLIIIV
                     LTYSLGAGFWNLILAFCITGWIGVAYSIRVQILRYRDLEYNLASQTLGTPMYKIAVKN
                     LLPQLVSVIMTMLSQMLPVYVSSEAFLSFFGIGLPTTTPSLGRFIANYSSNLTTNAYL
                     FWIPLVTLILVSLPLYIVGQNLADASDPRSHR"
     gene            complement(3633..5135)
                     /locus_tag="ACGEUI_001609"
     CDS             complement(3633..5135)
                     /locus_tag="ACGEUI_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8166429.1"
                     /translation="MKKYILNRIMRSLVSVVLVTALTYTIVYTLVPTSLIFKQDPNYN
                     KMTTTPDKKVAYENLTFQRMGYVNYFSSKELKDNASKVDSSVTTEATSANKAIYEKYI
                     DSLGNGWQLKRFPTSKQFYAIRNIPIYERVWNFFSNLVVIDHPWKIQDKDNPKLARYI
                     RLEKDKSVGWSLVGSGTKHKYLLYTNGKFPYLHQNFVTLNLGTSYPTYSNIPVLQVIS
                     QGQGRTALQDVTFPSGVTKKSSVDIYSRSYKNPKSLDDITKVNYGKGDSYTKTINNYV
                     DPSMIHNSFVIGFFGVMFSYIVGLPLGLFMARFKNTYFDSFSTATMTFMLALPSIAVI
                     YVVRFLGGMVGLPDSFPMLGASDPKSYILPALILGILNIPTTVIWFRRYLVDLQASDW
                     VRFARSKGLSESEIYRGHLFKNAMVPIVSGVPASIILAIGGATLTETVFAFPGMGKML
                     IDSIKSANNSMIVGLTFIFTVLSIVSLLLGDIVMTLVDPRIKLSTKKGGK"
     gene            complement(5200..7170)
                     /locus_tag="ACGEUI_001610"
     CDS             complement(5200..7170)
                     /locus_tag="ACGEUI_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8166430.1"
                     /translation="MKKSKWLAAVSVAILSVSALAACGNKNASGGSEATKTYKYVFVN
                     DPKSLDYILTNGGGTTDVITQMVDGLLENDEYGNLVPSLAKDWKVSKDGLTYTYTLRD
                     GVSWYTADGEEYAPVTAEDFVTGLKHAVDDKSDALYVVEDSIKNLKAYQNGEVDFKEV
                     GVKALDDKTVQYTLNKPESYWNSKTTYSVLFPVNAKFLKSKGKDFGTTDPSSILVNGA
                     YFLSAFTSKSSMEFHKNENYWDAKNVGIESVKLTYSDGSDPGSFYKNFDKGEFSVARL
                     YPNDPTYKSAKKNYADNITYGMLTGDIRHLTWNLNRTSFKNTKKDPAQQDAGKKALNN
                     KDFRQAIQFAFDRASFQAQTAGQDAKTKALRNMLVPPTFVTIGESDFGSEVEKEMAKL
                     GDEWKDVNLADAQDGFYNPEKAKAEFAKAKEALTAEGVTFPVQLDYPVDQANAATVQE
                     AQSFKQSVEASLGKENVIVNVLETETSTHEAQGFYAETPEQQDYDIISSWWGPDYQDP
                     RTYLDIMSPVGGGSVIQKLGIKAGQNKDVVAAAGLDTYQTLLDEAAAITDDNDARYKA
                     YAKAQAYLTDNAVDIPVVALGGTPRVTKAVPFSGGFSWAGSKGPLAYKGMKLQDKPVT
                     VKQYEKAKEKWMKAKAKSNAKYAEKLADHVEK"
BASE COUNT         2250 a         1403 c         1338 g         2241 t
ORIGIN      
        1 actttttttg tactttttta tttgcccgcg ttgtaaaatg aagtgcaaca aaaaagagaa
       61 aaagccttga actacacccc aaacgttaga caaaaaaatc taacgttcgg ggtatttttt
      121 atgaaattaa gtgatgaaag tacgaaatag acatcgttaa gaaagaaaaa aatatctact
      181 atccaccaga gataaagcaa gaaataatgg ataacgtttt gatagaaggc ccgccacaat
      241 accaagtatc acttgtttat ggcttgttac atgcaggaac tctcccaagt tgaatagcac
      301 aatataagaa aaacagttat actgttgaga aaacaagagc aagaccatca caaacaggac
      361 gtaaaccaaa agaaacctga agaaatgact aaattagagc ggcttcaaga agaaaatgaa
      421 tatctcagaa ctgaggttgc ttacttaaaa aaaccgagga actccgtttg aaagaagcat
      481 ctctgcaaag aagaagacaa aaaaaccatt ataaaaaatg gacaaagacg agttctgatt
      541 agattttctt ttaagaacgg ctgtattgac tcgttctacc tactattatc atcgattatt
      601 gcaacaataa acgtatcaaa ataaaactaa aaggacttag tcctctacaa tacaaaacta
      661 aatcctttac ttaaaataga gtagccaact tttggggtca gtgcatctct tttacaattc
      721 tttttgatat ttttcaattt ctgcttggtt tgcccaaaca aagtgatttg gtttgatttc
      781 aaccattgat ggcttatcta atgtataatc atgttggtct ggatgataga caacaagttc
      841 tttttgacgc tctaaaattg gatctgggat aggcacggct gataacaaag attgggtgta
      901 gggatgaatt gggttattaa acagttcttc tgtttctgca acttctacaa taaccccttt
      961 atggataacc gcaatacgat ctgaaataaa gcgaacgact gaaagatcat gggcaatgaa
     1021 gagataagtc aaaccttttt cggcttgcat tcttttaaga agattcaaga cctgtgcgcg
     1081 aacggaaacg tccaaagctg aaatcggttc atcagcaata acgaattcag ggttcattac
     1141 tagggctcta gcgataccga tccgctgacg ttgacctcct gaaaattcat gagggtagcg
     1201 cgtcaaatgc tctgatagca aaccaacttc ggccatcatg ttcttaattt tttctttacg
     1261 ttcttcctct gttttaaaca gattaaagtt ataaagacct tctgaaatga tgtagtcaac
     1321 ggttgcccgt tcattcaaac tagcagcggg atcttggaaa atcatttgaa ttttacgaat
     1381 gagctcattg gcttctgatt ttgattttct gccattaatt actttcccat cgtataaaat
     1441 ttgacctgaa ctagtatcgt tcaaaccaat aatagcacga ccaattgttg ttttaccact
     1501 cccagattct ccaactaaag aaaaggtttc tcctttttta ataaagaaat tagcattttt
     1561 aactgcaaca aattttttct ttccttctcc gaaggaaatt tctaggtctt ttacttcgac
     1621 taatttctca gacatttcct tcctcctgtt gtgacatttt tcttaagatt ttttgatgca
     1681 aatcttgaat gacttctggt ttttgaactt ttggagcctc tgggtgtaaa agccatgttt
     1741 tggcccaatg agtctcagat acgttaatcg ccggaggtgc tttttcaaag tctaaaacca
     1801 tagcatattc tgagcgaagt gcaaaggcat ctccgataat tggtgaataa agtgatggag
     1861 gcgttcctgg aatagcgtaa agttcaccag attcatctgc caactgcggt aagctagaca
     1921 gcaaactcca tgtatagggg tgtcttggat catagaaaat ctcttcgact gttccaaatt
     1981 caacaatttc tcctgcatac atgacagcca ctttatctgc aatgcttgcc acaacaccta
     2041 aatcgtgcgt aataaagata atggtgaaat gatattctcg ttgaagcgac ttcaataact
     2101 caacgatttg agcttgaata gtcacatcaa gggctgttgt tggctcatca cagataagaa
     2161 tatctgggcg acaagctaaa gcaatagcga taacaatacg ttgacgcatt cctcctgaat
     2221 actcaaatgg gtaatcttca aagcgttttt tggcatttgg gatacccact ttattcatgt
     2281 aatcaagggc catttcttta gctttggcat gacttacttt ttggtgctta ataatcactt
     2341 ctgtgatttg gctaccgata gttttaatgg gactaagact ggtcattggg tcttggaaaa
     2401 tcgttgcgat ttttgagccg cgaatctttg cccactcttt atttgttttt aaatctgtca
     2461 attcttgccc acgatagaca attgaaccat tagcaatgcg tccattagac tccaacatcc
     2521 ctgtaaaggt ctttgttaaa acagatttac ctgagcctga ttcccctaca aaagcaagga
     2581 cttctccttc aacaagttcc agtgagacgt tacggatagc tgttaaaaca cgatcacgca
     2641 catcaaattc taccaccaca tctttggcag ttaagattac attattttct tttgtcattt
     2701 taactcctat ctatgtgaac gtgggtcact ggcatcagcc aagttttgtc cgacaatgta
     2761 tagtggtaac gatactaaaa tcaatgttac taagggaatc caaaagaggt aggcatttgt
     2821 tgttaagttg cttgaataat tagcaataaa acgtcctaaa cttggagtgg tggttggtaa
     2881 accaatccca aagaaggata agaaggcctc agaagataca taaactggta gcatttgtga
     2941 caacatagtc atgataactg aaaccaattg aggcaggagg ttcttaacag caatcttgta
     3001 cattggtgtt cccaaagttt gactagcaag gttgtattct aaatcacggt aacgcaagat
     3061 ttgaacacgg atggagtagg cgacaccaat ccatccagtg atacagaaag ctagaatcaa
     3121 attccaaaat cctgcaccta atgaataggt caaaacaatg ataataagca tagaagggat
     3181 atttgagata atgttataaa tttcaatcat aactttatca aatgctttag aaactcccca
     3241 tatggctcct aacactaccc caatggtgat attaattagt gtcgctataa ctgagattaa
     3301 aatagaatta cgtgccccat accaaacacc atcaaacaga gattgcccat ttttgtcggt
     3361 tccaaaccag tactctgcat ttggccaaat ataacgcttt gaaaagtcat tgatattact
     3421 aacgtcatta aagtcgtagt tggcaaacat tggataaata aagctcatca tcaagactgt
     3481 cactaaaatt acgagcataa agactgtaga ttttttagaa aaaaactgac gaaacactga
     3541 tttccagtaa gaataagcag gggtatcaat cacttcggag gcttcactat cgcgctcaac
     3601 aaatcgaaat ttagatttat caatcgattc cattacttac ctcctttttt tgttgacaat
     3661 ttaatacgtg gatcgacaag ggtcatgaca atatctccta gtaaaagtga aacaattgac
     3721 aagacagtga agataaaggt taatccaaca atcatagaat tgttagcaga tttaatagaa
     3781 tcaattaaca ttttacccat acccgggaag gcaaagacag tctctgttaa tgttgcacca
     3841 ccaatagcaa ggataatact tgcaggaacc cctgacacaa ttgggaccat ggcatttttg
     3901 aagagatgcc ctctgtaaat ttcagattct gataatcctt ttgaacgcgc aaaacgtacc
     3961 caatcgctgg cctgaaggtc aacaagatag cgacggaacc agataaccgt agttggaata
     4021 tttaaaattc ctaaaataag ggctggtaaa atataagatt taggatcaga tgcccccagc
     4081 attgggaaac tatctggtaa accaaccatg ccacctagga agcgaacaac atagataaca
     4141 gcaatacttg gtaaagcaag catgaaggtc atggtcgctg ttgagaaact gtcaaaatag
     4201 gtatttttaa accgagccat aaataaacca agaggcaaac caacgatata ggaaaacata
     4261 acaccaaaaa aaccaattac aaaagaattg tgaatcattg atgggtcgac ataattattg
     4321 atggttttag tgtaggaatc tcccttacca tagtttacct tggtgatatc atccaatgat
     4381 tttggatttt tgtagctacg gctatagata tccactgacg atttcttagt gacacctgat
     4441 gggaaggtca catcttgaag agctgttcgt ccttgccctt gtgaaatgac ctgaagaaca
     4501 ggaatattgc tgtatgttgg ataagatgtc cctaagttta aggtaacaaa gttttggtga
     4561 agataaggaa attttccgtt agtatagagg agatacttat gttttgtccc cgaaccaaca
     4621 agtgaccagc caactgattt atctttttct agacgaatat acctagctaa ttttggatta
     4681 tctttatcct gaatcttcca agggtgatca ataacaacta gatttgagaa aaagttccaa
     4741 acacgttcgt aaatagggat attgcgaatt gcataaaact gtttgctagt ggggaagcgt
     4801 tttagctgcc agccattgcc aagagaatca atatacttct catagatagc tttgttagca
     4861 ctagttgctt ctgtcgttac actactatct actttagaag cattatcttt taattctttg
     4921 cttgaaaaat aattgacata acccatacgt tgaaaagtta gattttcata cgctactttt
     4981 ttatctggtg ttgttgtcat tttattataa ttagggtctt gtttaaagat aaggctggta
     5041 ggtactagag tatatacgat ggtgtatgtt aaagccgtta ccagaactac cgacactaac
     5101 gatcgcatga tacggtttaa aatatatttt ttcatagttg tcctcaatag ttcaaaagaa
     5161 ctccctcaca taaacgagaa agttcttttg aacatcgttt tatttttcaa cgtgatcagc
     5221 taacttctca gcatattttg catttgactt agcctttgct ttcatccatt tttcttttgc
     5281 tttttcgtat tgttttactg tgacaggttt gtcttgaagt ttcattcctt tatatgctag
     5341 aggaccttta gaccctgccc aagagaagcc cccgctaaat ggaacggctt tagtaactcg
     5401 tggagtgcca cccaatgcca caactggaat atctacggca ttatctgtaa ggtaggcttg
     5461 tgcttttgcg taagctttat agcgcgcatc gttgtcgtct gtaattgctg ctgcttcatc
     5521 aagaagagtt tggtaggtat caaggcctgc agctgccaca acatccttat tttgacctgc
     5581 tttgattcca agtttttgga taacagatcc accacctact ggactcatga tgtcaaggta
     5641 ggtccgtgga tcttgatagt ctggtcccca ccatgatgaa atgatatcgt agtcttgttg
     5701 ttctggggtc tcagcataga agccttgggc ttcgtgagtt gatgtttctg tttcaagaac
     5761 attgacaatg acattctctt taccaagaga tgcttcaaca gattgtttga aagactgggc
     5821 ttcctgaaca gttgctgcgt ttgcttggtc aacagggtaa tctaattgaa ctgggaaggt
     5881 tacaccttca gctgttaaag cttctttggc ttttgcaaac tcagcttttg ctttttcagg
     5941 attatagaaa ccatcttgag catcagctaa gttaacgtct ttccattcat ccccaagttt
     6001 tgccatttcc ttttcaactt ctgaaccaaa atcactttct ccaatggtca caaatgttgg
     6061 tgggacaagc atgttacgta aggcttttgt tttggcatct tgacctgcag tttgtgcttg
     6121 gaatgacgct cggtcaaaag caaactgaat agcttgacga aaatccttgt tgttaagagc
     6181 tttcttaccg gcatcttgtt gtgcagggtc tttcttagtg tttttgaaag aagtacggtt
     6241 caaattccat gttaaatgac ggatatctcc agtcaacatt ccgtaagtaa tgttatcagc
     6301 atagtttttc ttagctgatt tgtaggtagg gtcatttggg taaagtcgtg caacgctgaa
     6361 ctcacccttg tcaaagttct tgtagaacga acctgggtct gaaccatctg agtaagtcaa
     6421 tttaacagat tctatcccaa cattcttagc atcccagtag ttttcatttt tatggaattc
     6481 catagatgat tttgaggtga aggcgctcaa gaagtaagca ccattaacaa ggattgatga
     6541 tggatcggtt gtaccaaaat ctttaccttt tgacttcaaa aatttcgcat taactgggaa
     6601 aagcacacta taagttgttt ttgaattcca gtagctttca ggcttgttca aagtatactg
     6661 aacagtttta tcgtcaaggg ctttgacacc aacttcttta aaatctactt caccattttg
     6721 gtaagccttt aagtttttta ttgaatcttc aacaacgtaa agagcatctg atttatcgtc
     6781 aaccgcgtgc ttcaaaccag tcacaaaatc ttctgctgtt actggggcat attcttcacc
     6841 atcagccgta taccaagaga caccatcgcg aagagtataa gtataagtca gaccgtcttt
     6901 tgaaaccttc caatctttag caagtgatgg tactaaatta ccatactcat cgttttccaa
     6961 aagaccatca accatttgtg ttatcacatc agtcgttcca ccgccattag tcaaaatata
     7021 atccaatgat tttggatcgt taacaaaaac gtacttgtag gtttttgtag cttctgagcc
     7081 acctgaagca tttttattac cacaagctgc caaagcggat actgacaaga tcgcaacact
     7141 tacagctgcc aaccatttac ttttcttcat aaaagtcacc cctaaacttt atttttcatt
     7201 ttttggcaat tgctaaatga aattggttaa tt
//
LOCUS       DAXWIQ010000065         7065 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000065 DAXWIQ010000000
VERSION     DAXWIQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.065"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            163..1398
                     /gene="arcA"
                     /locus_tag="ACGEUI_001611"
     CDS             163..1398
                     /gene="arcA"
                     /locus_tag="ACGEUI_001611"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFF8166431.1"
                     /translation="MTAQTPIHVYSEIGKLKKVLLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRDEGIEVLYLETLAAESLVTPEIREAFIDEYLSEANIRGRA
                     TKKAIRELLMAIEDNQELIEKTMAGVQKSELPEIPASEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGTGVSLNHMFSETRNRETLYGKYIFTHHPIYGGGKVPMVYDRNETTR
                     IEGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHL
                     DTVFTMVDYDKFTIHPEIEGDLRVYSVTYDNEELHIVEEKGDLAELLAANLGVEKVDL
                     IRCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKIHGSEL
                     VRGRGGPRCMSMPFEREDI"
     gene            1498..1926
                     /locus_tag="ACGEUI_001612"
     CDS             1498..1926
                     /locus_tag="ACGEUI_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8166432.1"
                     /translation="MADKFDANDETRTVYAVVYDNDQPVSTGQFLAETKIEARLTRIV
                     TLADYCGCGYGAKVTEALETYTRREGFYQLTIHSELTAQTFYENLGYQSYGPKCLEDG
                     EYCQSLAKTILKWEKNMDIAMLIAIVGGLLGCYLYLTKNN"
     gene            1966..2979
                     /gene="argF"
                     /locus_tag="ACGEUI_001613"
     CDS             1966..2979
                     /gene="argF"
                     /locus_tag="ACGEUI_001613"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFF8166433.1"
                     /translation="MTQVFQGRSFLAEKDFTRAELEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEIVTLAEGYAKESGARILITEDA
                     DEAVKGADVLYTDVWVSMGEEDKFKERVELLQPYQVNMDLVQKAGNDKLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            3149..4642
                     /locus_tag="ACGEUI_001614"
     CDS             3149..4642
                     /locus_tag="ACGEUI_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFF8166434.1"
                     /translation="MTEEKKRGFRIPSSYTVLFIIIAIMAVLTWFIPAGAYETAKGGG
                     VISGTYKTVASNPQGFFDILMAPVRGMLGVEGTDGAIQVSFFILMVGGFLGVVNKTGA
                     LDTGIASVVRKNKGREKMLIAILIPLFALGGTTYGMGEETMAFYPLLIPVMIAVGFDS
                     IVAVAIILIGSQIGCLASTINPFATGVAADAAGVSIADGMIWRVIQWVILVGMSIWFV
                     YNYASKIEEDPSKSLVADKEEEHKELFQLQNSGEDLNKRQRNVLTIFTLTFVIMILSL
                     IPWEDFGIKFFTNINTWLTTMPILGGVIGKTMGAFGTWYFPEITMLFIMMGVLVAIVY
                     RMSEEDFFSSFLTGAGEFLGVAMICAIARGIQVIMNGGMITATILHLGETSLSGLSSQ
                     VFVILAYIFYLPMSFLIPSTSGLAGATMGIMAPLGQFSNVPAHLVITAFQSASGILNM
                     ISPTSAIVMGALALGRVDLGTWWKFIGKFIVMVMLVSVLLLVVATFF"
     gene            4659..5990
                     /locus_tag="ACGEUI_001615"
     CDS             4659..5990
                     /locus_tag="ACGEUI_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HFF8166435.1"
                     /translation="MESYITPKHQEACVAAIKQIVSYPSVCHEGENGTPFGQAIQDVL
                     EATLDLCQGLGFHTYIDPEGYYGYAELGDQKEVLAILCHLDVVPEGDRQLWKTDPFDC
                     VEADGCLFGRGTQDDKGPTMMALFATKALIDAGVTFNKRIRFIFGTDEETLWRCMNRY
                     NDVEEQATFGFAPDSSFPLTYAEKGLLQAKLVGKGSDKLSLEIGQAYNVVPARASYQG
                     DKLEALQKELDKLGFEYVVKEGELTVYGLAQHAKDAPDGINAMIRLAKALVVLEPNKT
                     LDFLANVIDEDGRALNIFGRIEDEPSGKLSFNAAGLTLTKDKAEIRLDIRIPVLADKE
                     KLVQQLSQKAQEYGLTYEEFDYLAPLYVPLDSELVTTLLSVYRKKTGDQSPAQSSGGA
                     TFARTMKNCVAFGALFPDAVQTEHQENEHIVLADAYRAMDIYAEAIYRLTR"
     gene            6010..6960
                     /gene="arcC"
                     /locus_tag="ACGEUI_001616"
     CDS             6010..6960
                     /gene="arcC"
                     /locus_tag="ACGEUI_001616"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFF8166436.1"
                     /translation="MTKQKIVVALGGNAILSTDASAKAQQEALISTSKSLVKLIKEGH
                     EVIVTHGNGPQVGNLLLQQAAADSEKNPAMPLDTCVAMTEGSIGFWLVNALDNELQAQ
                     GIQKEVAAVVTQVIVDAKDPAFENPTKPIGPFLTEEDAKKQMAESGASFKEDAGRGWR
                     KVVPSPKPVGIKEANVIRSLVDSGVVVVSAGGGGVPVVEDATSKTLTGVEAVIDKDFA
                     SQTLSELVDADLFIVLTGVDNVYVNFNKPDQAKLEEVTVSQMKEYITQDQFAPGSMLP
                     KVEAAIAFVENKPNAKAIITSLENIDNVLSANAGTQIIAG"
BASE COUNT         2108 a         1355 c         1513 g         2089 t
ORIGIN      
        1 ataaaaataa aaacaacgct caaaaaatgt aagcgattac tcaaaatagc tgtgagaaat
       61 accctatttt tatctttttt aaggagtaca ctaggaatat aagcaagagt taagaatctt
      121 gacggtgaat tcacaaaaac caattagaaa gaggtaattc ttatgactgc tcaaacacca
      181 attcatgttt attctgaaat tggaaaatta aaaaaagtgt tgctccacag acccggtaaa
      241 gaaattgaaa atcttatgcc agactacttg gaacgcttgt tgtttgatga tattccattc
      301 ttagaggatg cccaaaaaga acatgatgct tttgcccaag ctttgcgtga cgaaggtatt
      361 gaagtcttgt accttgagac cttagctgca gaatctcttg tcactccaga aattcgcgaa
      421 gctttcatcg atgaatacct aagtgaagct aatatccgtg gaagagctac taaaaaagcc
      481 attcgtgaat tgttgatggc tatcgaagac aatcaagaat tgattgaaaa aaccatggca
      541 ggagttcaaa aatctgaact tccagagatc ccagcatctg aaaaaggctt gactgacttg
      601 gttgaatcca attacccatt tgccatcgac ccaatgccaa acctttattt cacacgggac
      661 ccattcgcaa ctatcggtac aggtgtttct cttaaccaca tgttctcaga aacccgtaac
      721 cgtgaaaccc tttatggtaa atacattttc actcatcacc caatttatgg tggtggcaaa
      781 gtgcctatgg tttatgaccg taatgaaacc actcgcattg aaggtgggga cgaacttgtt
      841 ctttcaaaag atgtgcttgc ggttggtatt tctcaacgta cagatgctgc ttctattgaa
      901 aaattgttgg ttaacatctt taaacaaaac cttggcttca agaaagtatt ggcctttgaa
      961 tttgcaaata accgtaaatt tatgcactta gacactgtct ttaccatggt tgactatgac
     1021 aaattcacca ttcacccaga aattgaaggc gaccttcgtg tttactctgt cacttacgac
     1081 aatgaagaac ttcatatcgt tgaagaaaaa ggtgatttag cagaacttct tgctgctaac
     1141 cttggtgttg aaaaagttga ccttatccgt tgtggtggtg acaacttagt agcagcaggt
     1201 cgtgaacaat ggaacgatgg ttctaatacc ttgaccatcg caccaggtgt ggttgtggtt
     1261 tataaccgta acaccattac caatgccatt cttgaatcta aaggcttgaa gttgatcaag
     1321 attcacggaa gtgaattggt tcgcggtcgt ggtggacctc gttgtatgtc aatgccattt
     1381 gaacgtgaag atatttaata agctatggta aaggtggtta taggtcagaa gcctttttaa
     1441 agggcagcta gtgtttatct tcgcttctgt gtctttgtcc ttcaatgaaa ttttgtcatg
     1501 gcagataaat ttgatgccaa tgacgaaaca agaacggttt atgcagtcgt ttatgacaat
     1561 gaccagcccg tttcaacagg acaattttta gctgaaacga aaatagaagc acgattgaca
     1621 cgcattgtaa ccttagcaga ttattgtggt tgcggttatg gtgccaaagt cactgaagcg
     1681 ctcgaaactt ataccagacg agaaggcttt taccaactaa ccattcacag tgaactgaca
     1741 gcacaaacct tttatgaaaa cctaggttat cagtcctatg gtcccaagtg tttagaagat
     1801 ggtgagtatt gtcagtccct tgcaaaaacc attcttaaat gggagaagaa tatggacata
     1861 gcaatgctaa ttgcgattgt tggtggtcta ttaggctgct atctctatct cacaaaaaat
     1921 aattaatcaa aacagattac agttaacact cgaaggagac aatagatgac acaagtattt
     1981 caaggacgta gcttcctagc agaaaaagat tttacacgcg ctgaattaga ataccttatt
     2041 gatttttcag cacatttgaa agatttgaaa aaacgtggtg tgcctcatca ctacttagaa
     2101 ggtaaaaaca ttgccctctt gtttgaaaaa acatcaactc gtacgcgtgc agcttttaca
     2161 acagcagcca ttgacctagg tgctcaccca gaatacctcg gtgccaatga catccaactt
     2221 ggtaaaaaag aatcaacaga agacactgct aaagtattgg gtcgtatgtt tgatgggatt
     2281 gaattccgtg gctttagcca acgcatggtt gaagaattgg cagaattctc aggtgtgcca
     2341 gtttggaatg gtttgactga tgaatggcac ccaacccaaa tgcttgctga ctatttaaca
     2401 gtgaaagaaa actttggcaa acttgaaggc cttacccttg tttactgtgg tgacgggcgt
     2461 aataacgttg ccaattccct tcttgtgact ggtgctatcc ttggtgttaa tgttcatatc
     2521 ttctcaccaa aagaactttt cccagaagaa gagattgtga cattggctga aggctatgct
     2581 aaagaaagtg gcgctcgtat ccttatcaca gaagatgctg acgaagctgt taaaggcgca
     2641 gacgtgcttt acacagatgt ttgggtatca atgggtgaag aagacaaatt caaagaacgt
     2701 gttgaattgc ttcaaccata tcaagtcaac atggaccttg ttcaaaaagc aggaaatgac
     2761 aagcttatct tcttgcactg cttgccagct ttccatgaca caaataccgt ttatggtaaa
     2821 gacgttgctg aaaaattcgg cgtgaaagaa atggaagtca ctgatgaagt cttccgcagc
     2881 aaatacgctc gtcattttga ccaagctgaa aaccgcatgc acactatcaa agcggttatg
     2941 gcagcaaccc ttggtaacct ctttattcca aaagtgtaat aaggactggt actccttaac
     3001 tcatttcaca tatcaatgtg acccctttaa tttaacaatt taactactta aaaacgtact
     3061 ttaactacat gacaagtcgt tctggttttc gcgtccttga ccggaactag aacggcttat
     3121 ttttatgaaa aggtaagagg taatcattat gacagaagaa aaaaaacggg gttttagaat
     3181 tccttcttct tacaccgttc tttttatcat tatagccatc atggcagtgt taacttggtt
     3241 tattccagct ggcgcctacg aaaccgctaa gggcggtggt gttatttcag gaacctacaa
     3301 aacagtagca tccaatcctc aaggattttt tgacatcttg atggctcccg ttcgaggcat
     3361 gttaggtgtt gaaggcactg atggtgctat tcaagtgtct ttctttatct taatggttgg
     3421 gggcttttta ggagttgtca acaaaacagg tgctcttgac acagggattg cgtcagtggt
     3481 tcgtaaaaac aaaggtagag aaaaaatgct gattgctatt ttaatccctt tgtttgctct
     3541 aggtggaacg acctatggta tgggggaaga aaccatggcc ttttacccac ttttaattcc
     3601 tgttatgatt gcggttggtt ttgacagtat tgttgctgta gccattattt tgattggttc
     3661 tcaaattggg tgtctagcct ccactattaa cccatttgca actggtgtag cagctgatgc
     3721 tgctggtgtc agcattgcag atggaatgat ttggcgtgtt atccaatggg ttattcttgt
     3781 cggcatgtct atttggtttg tttacaatta cgctagtaag attgaagaag acccaagtaa
     3841 atcattggtt gcagacaagg aagaagagca taaggaactc ttccagttgc aaaattctgg
     3901 agaagactta aacaagcgcc aacgaaacgt tttgaccatt tttactttga cctttgtcat
     3961 tatgattctt agtttgattc cgtgggaaga ttttggcatc aaattcttta ctaatatcaa
     4021 tacttggtta accacaatgc ctatcttagg gggagttatt gggaaaacca tgggagcatt
     4081 tggtacttgg tatttcccag aaatcaccat gctctttatc atgatgggtg tcttagttgc
     4141 tattgtttat cgtatgagtg aagaagactt ttttagctct tttttgactg gtgcaggtga
     4201 atttttaggt gttgccatga tctgtgctat tgcacgcggt attcaagtta tcatgaatgg
     4261 tggtatgatt acagccacta tcttacactt aggtgaaaca agtctttctg gtttatcttc
     4321 tcaagttttt gtgatattag cttatatttt ctacctccca atgtccttct tgattccatc
     4381 aacatcagga cttgctgggg ctacaatggg aattatggca ccgcttggac aattctcaaa
     4441 tgtccctgct caccttgtta ttacagcctt ccagtcagct tctggaatct taaacatgat
     4501 ttctccaact tcagcaatcg ttatgggagc acttgcgctt ggtcgcgttg accttggtac
     4561 ttggtggaaa ttcattggta aatttattgt aatggtgatg cttgtaagcg tgctattact
     4621 tgtagttgca acattctttt aacagatagg aggttcctat ggaatcctat atcacaccta
     4681 aacatcaaga ggcctgtgta gctgccatta aacagatcgt ttcttaccct tctgtttgtc
     4741 atgaaggaga aaatggcaca ccttttggac aggccattca agatgttctc gaagcaacct
     4801 tagacttatg tcaaggtctt ggttttcata cttatattga tcctgaaggt tactatggct
     4861 atgctgagtt aggtgaccaa aaagaggtct tggctattct ttgccatttg gacgttgttc
     4921 cagaaggaga tcgtcagtta tggaagacag atccttttga ctgtgttgag gcagacggct
     4981 gtttatttgg tcgaggcact caagacgata aagggcctac tatgatggcg ctatttgcca
     5041 ccaaagcctt gatagatgca ggcgttacct ttaataaacg cattcgtttt attttcggaa
     5101 cagatgaaga aactttgtgg cggtgtatga atcgctacaa tgatgtagaa gaacaggcta
     5161 cctttgggtt tgctcctgat tcaagtttcc ctttaaccta tgccgaaaaa ggcctacttc
     5221 aggcaaaatt agtggggaaa ggttctgata aactgtcact tgaaatcgga caagcctaca
     5281 atgttgttcc tgcaagagcc tcttatcaag gtgataagct tgaggcttta caaaaagaac
     5341 tagataagct tggctttgag tacgtcgtca aagagggaga attaacggtt tatggtctcg
     5401 cccaacatgc taaagatgct cccgatggca tcaacgctat gattaggctt gccaaggctt
     5461 tagtggtcct tgaacctaac aaaacccttg attttcttgc taatgtcatt gatgaagatg
     5521 gcagagcact taatattttt ggcagaatcg aagatgaacc ttcaggcaaa ttaagcttta
     5581 atgccgcagg gcttacctta accaaagaca aggcagaaat tcgcctagat attcggattc
     5641 cagttttggc agataaggaa aaattagtcc aacaattaag ccaaaaagca caagaatacg
     5701 gcctaaccta tgaagaattt gattatttgg ccccattata tgtgccatta gacagtgaat
     5761 tggtaacaac cttgttatct gtctatcgga aaaaaacagg tgatcaaagc cctgcccaat
     5821 catcaggagg ggcaaccttt gctagaacaa tgaagaattg tgttgccttt ggtgcccttt
     5881 tccctgatgc tgttcaaacg gaacaccaag aaaatgaaca tattgtttta gcggatgctt
     5941 accgcgctat ggatatttat gcggaagcta tttaccgact aacgcgataa caagatatga
     6001 ggagtaatta tgacgaaaca aaaaatcgta gtcgcacttg gaggaaatgc tattctttca
     6061 acagatgcgt cagcaaaagc acaacaagaa gccttgattt ccacatcaaa atcactggtt
     6121 aaattaatta aagaaggtca tgaagtcatc gtgacccacg gcaatggccc acaagttggt
     6181 aatctcttgt tacaacaagc agcagcagat tctgaaaaga atccggctat gcctttggat
     6241 acttgtgttg ccatgacaga aggtagcatc ggtttttggt tagttaatgc cctcgataat
     6301 gaattgcaag cccaaggtat tcaaaaagaa gtggcagctg ttgtcactca agtgatcgtg
     6361 gatgccaaag acccagcttt tgaaaaccca acaaaaccaa ttggtccatt tcttaccgaa
     6421 gaagatgcca aaaaacaaat ggcggagtct ggtgcaagtt tcaaagaaga tgcaggtcgt
     6481 ggctggcgta aggttgtgcc atcaccaaaa ccagttggta ttaaagaagc caatgtcatc
     6541 agaagtctcg ttgattctgg cgttgttgtg gttagtgctg gtggtggcgg tgtgcccgtt
     6601 gtggaagatg ctactagcaa gaccctgact ggtgtggaag ctgttattga caaggacttt
     6661 gctagtcaaa ccctttcaga attggtggac gcagacctct ttatcgtttt gacaggagtt
     6721 gataatgttt acgtcaactt caacaaacct gaccaagcca aattggaaga agtgactgtc
     6781 tctcaaatga aagaatacat cacacaagac caatttgcac caggtagtat gttaccaaaa
     6841 gtggaagctg ccattgcttt tgttgaaaac aaaccaaatg ctaaagccat tatcacatcg
     6901 ctcgaaaata ttgacaatgt actatcagca aacgctggaa cacaaattat cgcagggtaa
     6961 tcaagaggat ttttagttct gtttaaaaga ctcatttaat ggtattttct gaagagtacg
     7021 actgacataa agagttgata ctgtttttaa aggaaaagag tgtgc
//
LOCUS       DAXWIQ010000066         6567 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000066 DAXWIQ010000000
VERSION     DAXWIQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.066"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..1346
                     /locus_tag="ACGEUI_001617"
     CDS             <1..1346
                     /locus_tag="ACGEUI_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFF8166437.1"
                     /translation="ANSEYMFLLLSIIVYYMTKIYIFSFLSDITLPVWKQLTILALAL
                     FFNQFPYLSPLLIDPLLFLVVLRQETKQLFSLKALFLAVAPSVLVDLLSRFMGTIVIP
                     YLFLSSGIYLGHIIFDLLAYLLIFPSFAIINYMIGKDYKMICQSGYSKRSHNFYQTLL
                     IFVLVYYVDIFVILGFTDPFLHFHHSLFVPTPYKLLFLMFILLLVYLLSYFNHSSKEY
                     LKNELRREQQAYMTNLEIYGKHLEKLYRDVRAFQSDYLSRIERLGQAIKSESITQIQD
                     IYAQTVHEANDYWDDKHYNISKLRKINISSIKSLLSAKIISAEKSGIDLNVEVPDNIK
                     ETYIPELDLLLLMSIFCDNAIEAALEAQQPHMSIAYFLLGDYQMFVVTNTTKKKVDIN
                     KIFEEGYSSKGSERGIGLSNAQRILKKYPYLSLRTKSFDKEFSQTLTMPKEEVDR"
     gene            1343..2626
                     /locus_tag="ACGEUI_001618"
     CDS             1343..2626
                     /locus_tag="ACGEUI_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFF8166438.1"
                     /translation="MMVIYSMALFYATIFINSWVIFAKVSAIKLSWKRVSIIGISFVI
                     ANMIFDKVILIDQLFFIIVSLLSAPKKKLFEHMFNGFFTILIVELLFRVIGSFFLPAV
                     LGFSIGQINNNLKLLELCYLFVLPIFYLFSYIFSIDLSLIRFISEDKMKKWVFWMNTA
                     MFSYYFFAHFLVTVQSGFLALYFQYRSILVFIYLAIFIWVIVKLDRFAKDQLSQKLTQ
                     AQNERIAYLENYNQSIEQLYREIRTVKHDSENILISLKDSIDSGDIDLITRVYDTVIQ
                     QSATSMMRTNYEISSLDNIKEAVIRSIMNSKLLEAQYLGIELYIEIPDVIDHLPIKLI
                     DLIVLFTGLVDNAIETAKGSRRPFLSIAYFKQDNKQLFIIENSTKTNRVDIAKRFDAQ
                     QQNSAHFLTVLDSYPQITLSTKSDHYRLRQLLEMR"
     gene            2630..3370
                     /locus_tag="ACGEUI_001619"
     CDS             2630..3370
                     /locus_tag="ACGEUI_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8166439.1"
                     /translation="MMNIFILEDDFIQQTRIESIVVGILKETRIPCNQLEVFSTPQKL
                     FESIQERGDHQLYFLDIEIGEYTRCGLELAAAIRQKDPNAVIVFVTTHSEFAPISFKY
                     KVSALDFIDKAGGQKQFKEQIEECIRYTYDMMSSRESKDMFLFETPQTRLKLPYKDIL
                     YFATATTPHKVCLWTQTERLEFYGNLSEIQAVAPKLFLCHRSYLVNLDKVVRIDKSKQ
                     LLYFENGDSCMVSRLKMKCLFERWEALR"
     gene            3910..4269
                     /gene="rnpA"
                     /locus_tag="ACGEUI_001620"
     CDS             3910..4269
                     /gene="rnpA"
                     /locus_tag="ACGEUI_001620"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFF8166440.1"
                     /translation="MKKTYRVKREKDFQAIFKDGKSTANRKFVIYHLNRGQDHFRVGI
                     SVGKKIGNAVTRNAVKRKIRHVIMALGHQLKSEDFVVIARKGVESLEYQELQQNLHHV
                     LKLAQLLEKGFESEEKH"
     gene            4235..5062
                     /locus_tag="ACGEUI_001621"
     CDS             4235..5062
                     /locus_tag="ACGEUI_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFF8166441.1"
                     /translation="MRKVLRVKKNIKIARIVPLVLLLVACGRGEVTAQSSSGWDQLVY
                     LFARAIQWLSFDGSIGVGIILFTLTIRLMLMPLFNMQIKSSQKMQDIQPELRELQRKY
                     AGKDTQTRMKLAEESQALYKKYGVNPYASLLPLLIQMPVMIALFQALTRVSFLKTGTF
                     LWVELAQHDHLYLLPVLAAVFTFLSTWLTNLAAKEKNVMMTVMIYVMPLMIFFMGFNL
                     ASGVVLYWTVSNAFQVVQLLLLNNPFKIIAERQRLANEEKERRLRERRARKKAMKRK"
     gene            5074..5988
                     /gene="jag"
                     /locus_tag="ACGEUI_001622"
     CDS             5074..5988
                     /gene="jag"
                     /locus_tag="ACGEUI_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFF8166442.1"
                     /translation="MVLFTGKTVEEAIETGLQELGLSRLKAHIKVISKEKKGFLGFGK
                     KPAQVDIEGISDKTVYKADKKATRGVPEDINRQNTPAVNSADVEPEEIKATQRLEAED
                     TKVVPLMSEDSPAQTPSNLAETVTETKAQQPSIPVEESEVPQDAGNDGFSKDIEKAAQ
                     EVSDYVTKIIYEMDIEATVETSNNRRQINLQIETPEAGRVIGYHGKVLKSLQLLAQNF
                     LHDRYSKNFSVSLNVHDYVEHRTETLIDFTQKVAKRVLESGQDYTMDPMSNSERKIVH
                     KTVSSIEGVDSYSEGNDPNRYVVVSLQR"
     gene            6303..6437
                     /gene="rpmH"
                     /locus_tag="ACGEUI_001623"
     CDS             6303..6437
                     /gene="rpmH"
                     /locus_tag="ACGEUI_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFF8166443.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLAARRRKGRKVLSA
                     "
BASE COUNT         2062 a         1080 c         1271 g         2154 t
ORIGIN      
        1 tggctaatag cgaatatatg tttttattat tatcaattat tgtctattat atgacaaaaa
       61 tttatatttt ttcctttcta tctgatatca cattaccagt ttggaaacaa ctaacgattt
      121 tggctttagc cctatttttc aatcagttcc cgtatttgtc gccattgcta attgatcctc
      181 tattattttt agttgtttta aggcaagaaa ccaagcaatt attttctttg aaagctcttt
      241 ttttggctgt tgcacctagt gttttggtgg atttgctatc tcgatttatg ggtacaatcg
      301 ttatccccta tctttttttg tcgagtggta tttatctggg tcatattatc tttgacttgc
      361 ttgcttatct gctgattttt ccaagttttg ctattattaa ttatatgatt ggaaaagatt
      421 acaagatgat atgccaatcc ggatattcga aacgatcgca taatttctat caaacattgc
      481 tgatttttgt tttggtttat tatgtggaca tttttgtcat tttaggattt acggacccct
      541 ttttacattt tcatcattct ctttttgttc ccacacctta taagttattg tttttgatgt
      601 ttatacttct tttagtttat cttctatctt attttaatca tagttctaaa gaatacctta
      661 aaaatgagtt gagaagagaa caacaagctt atatgactaa cttagagatt tatgggaaac
      721 atcttgagaa actctatcga gatgtaagag cttttcaaag tgattacctg agccgtatag
      781 agcgtttagg tcaagccatt aagagtgaat ccattactca gattcaagat atttatgccc
      841 aaacggttca tgaagctaac gactactggg atgataaaca ctataatatc tcgaagctta
      901 ggaaaattaa tatctcatcg attaagagct tattgtcagc taaaattatc agtgcggaaa
      961 aatcaggtat tgatttgaat gtggaagtgc cagataatat aaaagagact tacattcctg
     1021 agttggactt actattgttg atgtctattt tttgtgataa cgccattgag gcagctcttg
     1081 aagcacaaca acctcatatg tcaattgctt actttttgtt aggtgactat cagatgtttg
     1141 ttgtgacaaa tacgactaaa aaaaaggtag acattaacaa gatctttgaa gaaggctatt
     1201 cttcaaaagg ttcagaacgt ggtatcggcc tttctaatgc gcagcgtatt ctcaaaaagt
     1261 atccttattt atccttgcgg actaaaagtt ttgataagga atttagccaa accttgacca
     1321 tgccaaagga ggaggtggat agatgatggt tatatacagt atggctttat tctatgctac
     1381 catctttatt aacagctggg tcatttttgc aaaggtttct gctattaaac taagctggaa
     1441 acgggttagc attataggca ttagttttgt aattgctaat atgatatttg acaaagtcat
     1501 tttgattgac caactgttct ttattattgt tagtttgtta agtgctccga aaaagaaatt
     1561 atttgagcat atgtttaatg gtttctttac tatcttgatt gtcgaattac tgtttcgcgt
     1621 catcggatct ttctttttac cagctgtctt aggatttagc attggtcaaa ttaataataa
     1681 ccttaagctt ttagagttat gttatctttt tgtattacca atattttatc tgtttagcta
     1741 cattttcagt attgacttaa gcttaataag gtttatcagt gaagataaga tgaaaaagtg
     1801 ggtattttgg atgaatactg ccatgttttc ttactacttt ttcgcccatt ttttggtaac
     1861 tgtgcaaagc ggatttcttg cactatattt ccagtaccgg tctattttag tatttattta
     1921 tttagctatt tttatttggg ttattgtgaa attagatcga tttgctaaag atcagctgtc
     1981 acaaaaatta acacaagcac aaaatgaacg tattgcgtat cttgaaaatt acaatcaatc
     2041 gattgagcaa ctttaccgag aaatcagaac agttaaacat gattctgaaa atattttaat
     2101 tagtctaaaa gatagtatcg atagtggtga tatagatctg attactagag tttatgacac
     2161 ggttattcag caatcggcaa cttctatgat gcgcacaaat tatgaaatat cttctttaga
     2221 caatatcaaa gaggctgtga ttcgcagtat catgaattcc aaattgcttg aagctcagta
     2281 tttgggtatt gaactttata ttgagattcc agatgttatc gatcatttac ctattaagtt
     2341 aatcgactta attgtacttt ttactggact agtggacaat gctattgaga cagccaaagg
     2401 cagtagacgt ccttttttat ctattgcata ctttaaacaa gataacaagc aattatttat
     2461 tattgaaaat agcacaaaaa ccaatcgtgt ggatatagcc aaacgctttg atgctcagca
     2521 gcagaactca gcccactttt tgactgtttt agatagttac ccccaaataa cgctttcgac
     2581 taaaagtgac cattatcgat taagacaatt gttagaaatg agataaagta tgatgaacat
     2641 ttttattctt gaggatgatt ttatccagca gacacgcata gaatctatcg tagtaggcat
     2701 cttaaaagag actagaatac catgtaatca gcttgaagtg ttctcgactc ctcaaaaatt
     2761 atttgagagt atccaagaaa gaggagacca tcagctgtat tttttagata ttgaaattgg
     2821 tgaatatact cgttgtgggc ttgaattagc tgcagctatt cgacaaaaag atcctaatgc
     2881 cgttattgtg tttgtcacca ctcattcaga atttgctcct atttctttta aatataaggt
     2941 atccgctcta gattttattg ataaagcagg tggtcaaaaa cagtttaaag aacaaatcga
     3001 agaatgtatc cgatatactt acgatatgat gtctagtcgt gagtcaaagg acatgttttt
     3061 atttgagact ccacagacaa gattaaaatt gccctataag gatattttat actttgctac
     3121 agccacaact cctcacaaag tttgtttatg gactcagaca gaaagattag agttttatgg
     3181 taatctatct gagattcaag cagtagcacc gaaacttttt ttgtgccata gatcctacct
     3241 tgtgaatcta gataaggttg ttcgaattga taagagtaag cagctccttt atttcgaaaa
     3301 tggtgattct tgtatggtat cacgattaaa aatgaaatgt ctgtttgaac ggtgggaggc
     3361 tcttcgctaa agttaagaga gcaataacat tttaggacga aaaagcaaca tttcaggaaa
     3421 tgatgctctt attgaaacat ttttcgctat aataattagt gtaaataaag atttacgaag
     3481 tcatgagttt atcgagcaat caatccccaa ttcaatcaaa atattgtttt ctctctctcc
     3541 ataaacaaat agtatttttg atatgatggc tcggcagaca aaatgacgat gtcagttgtc
     3601 tttgtttgga tatcacatta gctgattgta aaatcagtat taaagcccgg cttgccgggt
     3661 tttttgatag gtaaatacag ggataagtag ctgacgtctt gtggattctg actttttatg
     3721 gttgttttct gaaggttatt cctttgtcga aagaagaact atactacgaa aagatggagt
     3781 gacaagaggc agttgaagca caaaaactag aaatagtaca gaaagaggct taatctgaaa
     3841 gggttagggc tttttaattt tttaggggat gtggtataat ggtaataatt tagatattgg
     3901 agttaaattt tgaagaagac ctatcgtgtc aagcgtgaga aagatttcca agccatattt
     3961 aaggatggaa aaagtacagc aaatcgaaaa tttgtcattt atcatttaaa tagaggccaa
     4021 gaccacttcc gtgtgggtat ttctgtcggt aaaaaaatag gaaatgcagt caccagaaat
     4081 gcagtcaaac gaaagatacg tcatgttatc atggcattag ggcatcagct gaagtcagag
     4141 gatttcgtgg tgattgcccg taagggtgtc gagtctttgg agtatcaaga gcttcaacaa
     4201 aatttacatc atgttttaaa gttagcacaa ttgcttgaga aaggttttga gagtgaagaa
     4261 aaacattaaa attgcaagaa tagtcccctt agttctatta ttagtggcct gtggtcgtgg
     4321 tgaggtaacg gcacaatcat ctagtggttg ggaccagttg gtttacttat ttgccagagc
     4381 aattcaatgg ctttcctttg atggttcaat tggtgttggc attattcttt ttacccttac
     4441 tatccgtctc atgctcatgc ctttgtttaa catgcaaatc aaatcaagcc agaagatgca
     4501 agacatccaa cctgagctta gagaattgca aaggaaatac gctggtaaag acacacaaac
     4561 gcggatgaag ttggctgaag aaagtcaagc cctttacaaa aaatatgggg ttaaccctta
     4621 tgctagtctc ttacccctct taattcagat gccagttatg attgccttat tccaagcctt
     4681 gacacgggta tctttcttaa aaacagggac tttcttgtgg gtggaattgg cacaacatga
     4741 tcatttgtac cttttaccag ttttggcagc tgtcttcact ttcttgtcca cttggttgac
     4801 caatctagcg gctaaagaaa aaaatgtcat gatgactgtt atgatttatg tgatgccgct
     4861 aatgatcttt ttcatgggct ttaacttggc tagtggagta gtgctctatt ggacggtctc
     4921 caatgccttt caagtggtac aactactgtt attaaacaat ccttttaaga ttattgcgga
     4981 aagacaacgt cttgccaatg aagaaaaaga acgccgtctt cgtgaacgtc gtgctcgtaa
     5041 aaaagcaatg aagagaaaat aggagtttag gttatggtat tatttacagg aaaaacagtc
     5101 gaagaagcta tcgaaacagg acttcaagag ttagggttat cacgccttaa agcgcatatc
     5161 aaagtcattt caaaagagaa aaaaggtttc ctcgggtttg gtaaaaagcc agcccaagtt
     5221 gatatcgaag gtatcagcga caagacggtt tataaggctg ataaaaaagc cactcgaggt
     5281 gttccagagg atattaatcg tcaaaatact cccgcggtca attctgctga tgtagaacct
     5341 gaagaaataa aagccactca aaggttagaa gcagaagata ccaaagtggt tcctctcatg
     5401 tctgaagata gtccagcgca aaccccttct aatcttgctg agacagtcac tgaaacaaaa
     5461 gcacaacaac cgtcaatccc agtcgaagaa tcagaagtgc cccaagatgc tggtaatgat
     5521 ggtttcagca aagatattga aaaagctgct caggaagtgt ccgattatgt gactaaaatt
     5581 atctatgaaa tggatatcga agctaccgtt gaaaccagca acaatcgtcg ccaaatcaat
     5641 ttgcagattg aaacgccaga ggcgggacgt gtgattggtt accatggtaa ggtcttgaaa
     5701 tccttgcagt tgttagctca aaatttcttg cacgaccgct attcaaaaaa tttttcagta
     5761 tccttgaatg tccatgatta tgtggaacat cgaacagaaa ccttgattga ctttacgcaa
     5821 aaagttgcca aacgcgtttt ggaatctggt caagattaca ccatggatcc catgagtaac
     5881 agtgagcgta aaatcgttca taaaaccgtt tcatccattg aaggggttga tagttattct
     5941 gaaggcaatg accctaaccg ctatgtggta gtcagtcttc aacgttagat cctaaaaaga
     6001 atctttggta gttagtttag ctatcgaaga ttttttgtat ggccagctta accgtttgtt
     6061 ttactgatct cattgattat tgttgctatt gataaggttt caacggtaag gcaaaggaac
     6121 agtgtcaaag aagagacact ttatgaaatg agcggcctct taaatgagag agaaactgtt
     6181 gaaaaacgaa aataaatctt gacagtagac cctctttaag atagaatatt atagtatgtt
     6241 tagtaactga tatcacaaat agccggctaa acgaatacaa aatctatgag gaggtattca
     6301 ctgtgaaacg tacttatcaa ccaagtaaaa tccgtcgtca acgtaaacac ggattccgtc
     6361 accgtatgtc aactaaaaac ggacgtcgcg tgcttgctgc acgtcgtcgt aaaggacgta
     6421 aagtattatc agcttaattg caaaaaatac aactaaagta aaccaagagt gctcgaggct
     6481 cttggttttt taacgttttc taatcggaaa tttgcaatgt ataagtatgg tgtcaactcc
     6541 ttaccttttt atttgttgta tgaaaga
//
LOCUS       DAXWIQ010000067         6335 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000067 DAXWIQ010000000
VERSION     DAXWIQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.067"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..198
                     /locus_tag="ACGEUI_001624"
     CDS             <1..198
                     /locus_tag="ACGEUI_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFF8166444.1"
                     /translation="PDNGMMESFFGILKSEMFYGLETTYQSLDKLEEAITDYIFYYNN
                     KRIKAKLKGFSPVQYRTKSFQ"
     gene            complement(498..1370)
                     /gene="nadC"
                     /locus_tag="ACGEUI_001625"
     CDS             complement(498..1370)
                     /gene="nadC"
                     /locus_tag="ACGEUI_001625"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFF8166445.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDRIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGV
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            1594..2904
                     /locus_tag="ACGEUI_001626"
     CDS             1594..2904
                     /locus_tag="ACGEUI_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFF8166446.1"
                     /translation="MIHLIIISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KALLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPDALYMILYLIAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHSLIGAQAVNWGASGGANLITSGSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYIRYSKGRTNSSLTIDQPPILS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRQTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWAIIIMLCGMSLLMSLAVKSGLVTLIGHLITTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPTLFPIIATISAQSPHIDIRLLTTATIIGALSTNISPFSSAGSL
                     IQLSLPHIEERSLAFKKQILLGVPISLSLALLTIWILMLLASLS"
     gene            2982..4132
                     /locus_tag="ACGEUI_001627"
                     /pseudo
     CDS             2982..4132
                     /locus_tag="ACGEUI_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558257.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            4259..4414
                     /locus_tag="ACGEUI_001628"
                     /pseudo
     CDS             4259..4414
                     /locus_tag="ACGEUI_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4461..4991)
                     /locus_tag="ACGEUI_001629"
     CDS             complement(4461..4991)
                     /locus_tag="ACGEUI_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFF8166447.1"
                     /translation="MSNFISPVYEIKSIPIEKISPNDYNPNSVAPPEMKLLYDSIKSD
                     GYTMPIVCYYDKEEDRYSIVDGFHRYRIMLDYSDIYERESGRLPVSVIDKSLDYRMAS
                     TIRHNRARGSHDVDLMSQIVKDLHECGRSDNWIAKHLGMDKDEILRLKQITGLASLFK
                     DHEFNQSWEPEELLNE"
     gene            complement(4984..6318)
                     /locus_tag="ACGEUI_001630"
     CDS             complement(4984..6318)
                     /locus_tag="ACGEUI_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFF8166448.1"
                     /translation="MKKYLNQNVYDALIERLHFLFNEFPIVYISFSGGKDSGLLLNIL
                     LDFRDKYYPDREIGVFHQDFEAQYSLTTKYVQETFTSLEGRKKVSLYWVCLPMATRTA
                     LSSYEMFWYPWDDKTEDIWVRPMPSQDYVINLENNSITTYRYKMNQEDLAKQFGRWYK
                     QIHGNQKTVCILGNRASESLHRYSGFINKKYGYQKECWITKQFKDVWTASPLYDWSVE
                     DIWHAYYKFSYSYNELYDLFYKAGLKPSQMRVASPFQDYAVDSLNLYRIIDQETWVKL
                     LGRVQGVNFSNIYGRTKAMGYKSIALPKGHSWKSYTQFLLSTLPVRLRNNYVRKFNKS
                     IDFWHKTGGGLAEETINELIEKGYRIARNGISNYTSFKHSRVIFLDQIPDDTDDIVTT
                     KDIPSWKRMCFCILKNDHICRTMGFGLTREQQKIVAFLKEKYKTSLKELESE"
BASE COUNT         1970 a         1355 c         1017 g         1993 t
ORIGIN      
        1 ccggataacg gcatgatgga atccttcttt ggcattctca aatctgaaat gttttatgga
       61 ctcgagacaa cttatcaatc acttgacaag cttgaagaag ctattacaga ttacattttt
      121 tactacaaca acaaacgaat taaagcaaaa ctaaaaggat ttagtcctgt ccaatacaga
      181 actaaatcct ttcaataatt atttgtccaa ctttttgggg tcagtacact cctagttaac
      241 gactttgatt gattttatta tgatgaatcg tgatttctga aaacaaaatt ttcttcatgg
      301 atttggcgtg gaacttttac aataaaaaca ctaacctctc cctatcgttt tttcaggtga
      361 atgcttggaa ttggtataat aagcggacac gaatcaggtg aaaacaggga aagtatttga
      421 aaaggcgttt aaactatctg acataaaact cgttctagag gaaagacatc ctttaaaaag
      481 ttagtttatt ttgcaactta gacatcaagg taggttaaac ccttcatgga aaaatcgaga
      541 ctcttagcac tatgggttaa actaccactg gagacgtaat caatcgctaa accacgaaaa
      601 cggctaatag tggtcatatc aatatttcca gaacattcaa tccgagaacg tcctgcaatt
      661 agagtaatgg cctgttcaat ctgttccaat gacatattat ccaacatgat aatatcaaca
      721 cccgccgccg cagcttcttc ggcagcagca aggctttcca cttccacctc gaccattttc
      781 acaaaagggg cataggcacg cgcttgagca atcgcctttt gaacactacc tactgccgca
      841 atgtgattgt cttttagcat gatagcatct gataaattaa agcgatgatt atagccaccg
      901 ccaactctca cggcatattt ctcaaaaaga cgtaaattag gagtggtttt tcgagtatca
      961 aataccttaa tgcgatcatc gcctaaggct tctacataag cagctgtcat cgaagcaatc
     1021 cctgataaat gttgtaaaaa attcaaggca acgcgttcac atgttaagag gcttctcacc
     1081 gagcctatga tttctaaaac caaatcgcca ctagtcaaac gatccccatc cttaaattga
     1141 tgaggattct ggaaggtcac ctcggcatca aatagggtaa aaaccctttg aaaaacggtt
     1201 agccccgcta aaacaccagc ttccttggca aaaagcgaca ccttggcttg gccatgatga
     1261 tcaaaaatgg cattggtact gtaatcttcg gaatgaacat cttctcgcaa ggctgctttc
     1321 aatgtatcat ctatttgaaa aggggttaaa tcagttgaaa tgattgacat cactaagtcc
     1381 tctattttta atatcacttg taagcactag aggcaattgc tattaacttc cgtaggaaat
     1441 cacaaacctg aatacctcat tttaaatccc aacaagtgtc aagacaattg taattttttt
     1501 gtgtataata gtcaagtacc aataggaaac aggtggtctt ttgagcgcca aaattgccaa
     1561 cctattttca tattctaact aaaggacaat tcaatgattc atttaataat aattagcgcc
     1621 attgctctag ccattggaat tggttaccgc accaaaatca atattggcct gctggctatt
     1681 gctttttctt acctcatcgc aaccactctc atgggattaa gccccaaagc acttcttcat
     1741 ttttggccaa cctcactctt ttttaccatt tttagcgtct ctctctttta taacgttgca
     1801 acaactaacg gcactcttga tgttttggct caacacattc tctaccgcac acgcacccac
     1861 cctgacgccc tctacatgat tttatacctg atagcaaccc ttttgtctgc tttaggtgct
     1921 ggatttttca ctactatggc cgtttgctgt cctctagcga ttaccctctg tcaaaaagcg
     1981 gacaaacact ctttgattgg agctcaagcc gtcaattggg gagcttcagg aggggctaat
     2041 ttgataacca gtggctcagg cattgtcttt caaggcctgt ttaagcaaat gggatgggaa
     2101 gagcaagctt tttcacttgg caatcatatc tttatcgtca gcattatcta cccactcatc
     2161 gtcttgcttt tattatcttg ttacatccgc tacagtaagg gaagaacaaa ctcatccttg
     2221 actattgacc aaccaccaat cctcagcaag gtccaaagac aaaccacctt gctcatgatc
     2281 agcagcatgg ttttggtttg gctttttccc ttgttgcatc tcattttccc aaacatcgca
     2341 tggatagcta cttatcgaca aacatttgat attggctttg tctcaatact catggtctgc
     2401 ctcgccttaa ggctcaaatt agggaagcaa gaagccattt tggctaaggt tccctgggct
     2461 ataattatca tgctctgcgg catgagctta ctaatgtctc tagcggtcaa gtcaggttta
     2521 gttacactga tagggcacct cataacaaca accattcctc atttctggtt acctctgttc
     2581 ttttgtgtga tagccggagt gatgtcactc tttagttcca ccctatccgt tgtagcccca
     2641 actctctttc ccatcattgc taccatcagt gctcaaagtc ctcacattga tattcgctta
     2701 ctcacaacag ccactattat tggcgctcta tctactaata tctctccttt ttcatcagct
     2761 ggttccctca ttcaactctc ccttccccat atagaggaac gcagtcttgc tttcaaaaaa
     2821 caaattcttc tgggtgtgcc catcagctta agtttggcac tcttgaccat ctggattctc
     2881 atgctgctag cttccttaag ttagatttat tgtctaactt aaggttggac ttggtataat
     2941 aaagtagaca caaaattaag tggaaacgag gaaaagtcat tatgccaaga aaaacctttg
     3001 ataaagcctt caaactctct gctgtaaaac tcatccttga ggaagagtag cctgtaaaaa
     3061 tggctagttc aactttaaaa attcatccca atagtcttta tcgatggatt caagaatatg
     3121 aaaaatatgg agaaagtgcg ttcccaggac atgggagcgc acttcgtcat gctcaatttg
     3181 agacaaaaaa acttgaaaaa gagcacaaac tgctacagga ggaattagct cttctaaaaa
     3241 agttccaggt ctttttgaag ccaaaccgga aataaaattt cagtttctga aggaaaatag
     3301 tgttagcttg aatagtcatc acgcttgtca aactcttcaa gtctcccgtt caggtttcta
     3361 tgcttattta aaacgtcgtc cctcttcaag gcatgttgag aatgaagctt tgaaggaaat
     3421 gattaaagcc attttctatg aacataaaga gcgttacgga agtgtacgaa ttacccaaga
     3481 actttgtaga cgtggcatcc atgtcaatta taaacgtgtt ggtagactcc ttcatcagct
     3541 aggtctctat gctaaaggaa gtcgatatca atataaatac tataaccgtc gacgttcttc
     3601 attaactcgt cccaatcttg ttaatcaatg tttccaagcg actggtaaga ataaattatg
     3661 gctaggagac ctaacctaca ttcctacaca agaaggaata ctttatgtgt ctgtctttat
     3721 agacgtttat agtagaaaga ttgttggttg ggcaatgggc cgacgtatgc aagacaagtt
     3781 ggtaacagaa gctttcaatc aagcttataa tagagaaaag cctaaagaag gggtaatcgt
     3841 ccatacagat cagggttctc aatataccgg agcatgattt caggacctac ttagacagaa
     3901 aaaatgtaag tccaggatga gtcgaaaagg taatccatac gataatgcac tcatggaatc
     3961 cttttataaa acattaaaaa gagagcttgt caatgatgct catttcgcaa ctattgagca
     4021 agctcagctt gagattttta aatatagtga gacttactat aaccctaaac gtctgcattc
     4081 agctttaggg tatctctcac ctgttgaatt tgaaaaaata gttactcatt agcttaactt
     4141 tatgtctatt ttttcttgac aagtccaggg tatgaagtcc ctttatacac atactttcaa
     4201 aacagctctc aatcctctgt ccgctacaaa aatacgtcac cgctgccctc cagctgcctc
     4261 attcaacgac aaacttgaag ccacaaacag actcatcaag ggcattaagt acaatgcctt
     4321 tagatttcag aactttgaca actttaaaac caaaatcctc atcgctttga acatccaaaa
     4381 agagagaacc aatctgattc tctctcgttg ttaatgataa gtcacccact acagttgaca
     4441 atgagtctct tacttcctgt ttactcattt aatagctctt ctggttccca agattgatta
     4501 aactcatggt ccttaaaaag agaagccaat cctgttattt gtttcagtct taaaatttca
     4561 tctttatcca ttcctaaatg ttttgcaatc cagttatcac ttcgcccaca ctcatgaaga
     4621 tcctttacaa tctgactcat caaatcaaca tcatgacttc ctctagctct attatgtcga
     4681 atggtagaag ccattcgata gtctaatgac ttatcaataa cagaaacagg taatctccca
     4741 ctttcccttt cataaatatc tgaataatct agcattatac gatatctatg aaaaccatca
     4801 acaatactat accgatcctc ttccttatcg taatagcaaa caatcggcat agtataacca
     4861 tcagatttta tactatcata caataatttc atttcaggcg gagcaactga attcggatta
     4921 taatcatttg gagaaatctt ttcaattgga attgatttaa tctcatagac tggactaata
     4981 aaattactca ctttctaact cctttaaact cgtcttatat ttctctttta aaaaggctac
     5041 tattttttgt tgctctcttg tcaaaccaaa ccccatagtt cgacaaatat gatcgttttt
     5101 taaaatacag aaacacattc gtttccagct tggtatatcc tttgttgtta caatatcgtc
     5161 tgtatcatca ggaatttgat ctaaaaaaat aacccttgaa tgtttaaaag aggtataatt
     5221 tgaaatacca tttctagcaa tacgatatcc tttttcaatt agttcattaa ttgtttcctc
     5281 cgcaagtcct ccgcctgttt tatgccaaaa gtctattgat ttattaaatt ttctaacata
     5341 attatttctt aacctcactg gtagcgttga taaaagaaac tgagtgtacg acttccacga
     5401 atgacccttt ggtaaagcaa ttgatttata tcccattgct ttagtacgtc cataaatatt
     5461 cgaaaagtta actccttgaa cacgtcccaa aagctttacc caagtttctt gatctattat
     5521 tctataaaga tttaggctat caacagcata gtcttgaaaa ggagatgcaa ctctcatttg
     5581 tgatggtttt aaacccgctt tatagaatag atcataaagt tcgttataag aataagaaaa
     5641 cttatagtaa gcatgccata tgtcttccac tgaccaatca tataagggag atgctgtcca
     5701 gacatctttg aattgtttgg taatccagca ttccttttga taaccatact ttttattgat
     5761 aaaaccacta tatctgtgta aggattctga agcacggttt cctaatatac aaactgtttt
     5821 ttgatttccg tgaatttgtt tataccacct accaaattgt ttcgctaaat cctcctgatt
     5881 cattttataa cgataagtag taattgaatt attttccaaa ttaataacat aatcttgaga
     5941 tggcattggt cttacccaga tatcttctgt tttgtcatcc cacggatacc aaaacatttc
     6001 ataactacta agtgcagttc tagtagccat cggtaagcac acccaataca aagaaacttt
     6061 ttttctacct tctaaagaag tgaatgtttc ttgaacatat tttgttgtta aactgtactg
     6121 tgcttcaaaa tcttgatgaa acacacctat ttctctatcc ggataatatt tatctctaaa
     6181 atccagtaaa atattaagaa gtaacccact atcttttcca cctgagaagg aaatatagac
     6241 aataggaaat tcattaaaca aaaaatgtaa tctttctatc aatgcatcat aaacattttg
     6301 atttaaatac tttttcacta aacttcctca ttatg
//
LOCUS       DAXWIQ010000068         6007 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000068 DAXWIQ010000000
VERSION     DAXWIQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.068"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..186
                     /locus_tag="ACGEUI_001631"
     CDS             <1..186
                     /locus_tag="ACGEUI_001631"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166449.1"
                     /translation="MIDRLTLQNNFLMVNCSLCEITAEKYEARQQVVIYSSNLCCLGE
                     NPLHESGFFKPLFPQKI"
     gene            202..813
                     /gene="rpsD"
                     /locus_tag="ACGEUI_001632"
     CDS             202..813
                     /gene="rpsD"
                     /locus_tag="ACGEUI_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFF8166450.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVDPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(967..1500)
                     /locus_tag="ACGEUI_001633"
     CDS             complement(967..1500)
                     /locus_tag="ACGEUI_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8166451.1"
                     /translation="MVNRKENTKQAILKAMVMLLKTESFDDITTVKLSKRAGISRSSF
                     YTHYKDKYEMIDYYQQTFFHKLEYIFEKKYQNKEQAFLEVFEFLQREQLLSSLLSANG
                     TKEIQAFIINKVRLLITTDLQDKFSTEELSQTEKEYQSIYLAHAFFGVCQSWIAKGKK
                     ESPQEMTQFVLKMLTST"
     gene            1635..3908
                     /locus_tag="ACGEUI_001634"
     CDS             1635..3908
                     /locus_tag="ACGEUI_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFF8166452.1"
                     /translation="MLEELKTLIKNPKLMITMIGVALVPALYNLSFLGSMWDPYGRVN
                     DLPIAVVNHDKPAKRADKSLTIGNDMVDKMSKSKDLEYHFVSAKQAQEGLKEGDYYMV
                     ITLPEDLSQRAATLLNTEPQKLTIRYQTSKGHGMVAAKMGETAMAKLKESVSQNITKT
                     YTSAVFSSMTDLQSGLKEASAGSQALASGAKTAQAGSQTLSTNLAALTGASQQFQQGT
                     GRLTSGLTTYTDGVNQVKNGLGTLSTDIPNYLNGVSRLSQGASQLNQGLSQLTQATTL
                     SDEKAKGIQSLIVGLPVLNQGIQQLNTELSTLQPPNLNADELGNSLGAIAQAAKQVIA
                     EETAAQNEELSALQATSVYQSLTAEQQGELAAALSQSDKSQTVSAAQTILSSVQTLST
                     SLQSLSQEDQSKQLEQLKEAVAQIANQSNQALPGASSALTELSTGLAKVNGSLNQQVL
                     PGSNQLTTGLAQLNRYNTAIGSGVIKLSEGANALSSKSGELLDGSHQLSEGATKLADG
                     SSQLSQGGHQLTSGLTELSTGLSTLNGSLAKASQQLSLVSVTDKNAKAVAKPLVLNEK
                     DKDGVKTNGIGMAPYMIAVSLMVVALSTNVIFANSLSGRPVKDKWDWAKQKFVINGFI
                     STMGSIVLYLAIQLLGFEARYGMETLGFIMLSGWTFMALVTALVGWDDRYGSFASLVM
                     LLLQVGSSGGSYPIELSGAFFQKLHPFLPMTYVVSGLRQTISLSGHIGVEVKVLTGFL
                     LAFMVLALLIYRPKKTV"
     gene            complement(4026..5105)
                     /locus_tag="ACGEUI_001635"
     CDS             complement(4026..5105)
                     /locus_tag="ACGEUI_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFF8166453.1"
                     /translation="MKNISRKCFMTSVVCIILGGILLGTGYATGGLQDIKHQTAPKKV
                     IKTFDQITALDIDSSASTITVETGPVQRPTVTYYTHPKLIDPIVTTVTGKTLSLSQTP
                     KDVVITGGIEILGFTLNNSRQEKNYRSITITVPEKTSLNEVKASNVPHTTLSNLTVQD
                     MQFDGNLTLLHTKVKKATITGMLEATKSQLTNLELKADYSFSNLTDSSVENGTISLGN
                     GQLTTKDTTLKAVNIQSLHPGGIEAERTTLENVTFTVSKSKEEEENDYYDNDAIFTAH
                     ALTLKGTNTITGGDIDVDITLTKAKAIAYRARTENGKVSLGSQLTPAKIGKESTSDVI
                     SYVAENKAATGNLTVNLNKGDITIK"
     gene            complement(5102..5689)
                     /locus_tag="ACGEUI_001636"
     CDS             complement(5102..5689)
                     /locus_tag="ACGEUI_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFF8166454.1"
                     /translation="MTRTEYLAELDKYLRKLPREDYHEAMEYYIEYFDEAGPDKESQV
                     IDDLGNPKEAASEIISNVLGKHLATPEKTPKNRATIIGLTILSLFAAPIALPVLLALI
                     LFIIACLMLGLTAIFAAYIFGLAGILVAGVTLFESLTLIGSSIPAQAMGIGSALLCFG
                     GGLLIWIIATAFLRFSGRAFVAFIKWISNKKGAQA"
     gene            complement(5676..6002)
                     /locus_tag="ACGEUI_001637"
     CDS             complement(5676..6002)
                     /locus_tag="ACGEUI_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8166455.1"
                     /translation="MYFPVSATLIEFLILAIVEKNDSYGYDISQTIKLVANIKESTLY
                     PILKKLEKAGFLTTYSQEHQGRKRKYYAVTSSGRAQLIFLKKEWQSYKFALDGIIEGS
                     LRHDKD"
BASE COUNT         1799 a         1130 c         1181 g         1897 t
ORIGIN      
        1 atgatagata gactgacttt acaaaacaac tttcttatgg taaactgttc tttgtgcgaa
       61 ataacagcag aaaaatatga agctcgtcaa caggtggtta tctattcaag taacctttgc
      121 tgtttaggcg aaaaccccct gcacgaatca ggatttttta aaccgttatt tcctcaaaaa
      181 atttagagga ggacatgtaa tatgtcacgt tatactggtc catcatggaa acaatcacgt
      241 cgccttggtt tatcacttac aggcacaggt aaagaattgg cacgtcgtaa ctacgttcct
      301 ggtcaacacg gtccaaacaa ccgtagcaaa ctctcagaat atggtttgca gttagctgag
      361 aaacaaaaac ttcgtttctc atacggttta ggtgaaaaac aattccgtaa cttgttcgtt
      421 caagctacaa aaatcaaaga aggaactctt ggtttcaact ttatggttct tttagaacgt
      481 cgtcttgaca acgttgttta ccgtctaggt ttagcaacta ctcgtcgtca agcacgtcaa
      541 ttcgttaacc acggtcacat ccttgttgac ggtaaacgcg ttgatatccc ttcataccgc
      601 gttgatccag gtcaagtgat ttcagttcgc gaaaaatcaa tgaaagtacc tgcaatcctt
      661 gaagctgttg aagctactct tggacgtcca gctttcgtat catttgatgc tgaaaaactt
      721 gaaggttcat tgactcgcct tccagaacgc gatgaaatta acccagaaat caatgaagca
      781 cttgtcgttg aattctataa caaaatgctt taattttaag attaaactta caaaaagcct
      841 atttaatagg ctttttttgt atctcataac aaatttatcc tgatcccttc attttactat
      901 ggcttctaaa aaagttatca ctaaggctga tgacttgtaa aatgatactt tcgtaagggt
      961 tattatttag gtactcgtaa gcattttgag aacgaattgt gtcatttctt gaggtgattc
     1021 ttttttacct ttagcaatcc aactttgaca gacaccaaaa aaagcatggg ctaagtagat
     1081 actttggtat tctttttccg tttgagataa ctcttcggta ctgaacttgt cctgtaaatc
     1141 agtagtgatg agtaggcgaa ctttattgat gataaaggcc tgtatttctt tagtaccgtt
     1201 agcagacaaa agggaagaga gaagctgttc tctttggaga aattcaaata cttcaaggaa
     1261 ggcttgttct ttattttgat attttttctc aaaaatatat tctaatttgt gaaaaaaggt
     1321 ttgctgataa tagtcaatca tttcatattt atctttatag tgtgtgtaaa aactagagcg
     1381 actaattcca gctcttttag ataatttaac tgtggtaata tcgtcaaaac tttcggtttt
     1441 tagtaacata accattgctt ttaaaattgc ttgcttcgta ttttctttac ggttgaccat
     1501 ctaccatctc tccttttgaa cacttttaga cataatgtcc aaaagtgaat ttttttctct
     1561 tgtaaattta aatttaaatt gtataatata ttttatcata atttttgaac agtatgtcca
     1621 ttttaggaga aaatatgtta gaagaattga aaacacttat taaaaatcca aaattaatga
     1681 ttacaatgat tggtgtggcc ctagtgcctg ccttatataa tttatccttt ctaggctcaa
     1741 tgtgggatcc ttatggtcgg gtcaatgacc ttcccattgc tgttgttaat catgataagc
     1801 ctgcaaagag agctgataag tcattgacaa ttgggaatga tatggtggac aagatgtcta
     1861 aaagtaaaga tttagagtat cattttgtct cagctaagca agctcaagag ggacttaaag
     1921 aaggtgatta ttatatggtt attactttac ccgaagatct ttctcaacgg gcagcaacct
     1981 tattaaatac cgaaccccaa aaattaacta tccgttacca aacgagtaaa ggacatggaa
     2041 tggtcgctgc taagatgggg gaaacagcga tggctaagct gaaagagtct gtttcgcaaa
     2101 acattacgaa gacttatacc tcagcagttt ttagcagtat gacagacctc caatcaggat
     2161 taaaagaagc ctcagctgga agtcaagcac ttgcttcagg agcgaagact gctcaggcgg
     2221 ggagtcaaac actttcgacg aacttagcag ccttaacggg tgctagccaa cagtttcaac
     2281 aaggtactgg tcgattgaca tcaggtttga ctacctatac agatggtgtc aaccaagtca
     2341 agaatgggtt aggaacatta tcaacggaca tccccaatta tctgaatggg gtttctaggt
     2401 tatcccaggg agcttctcag cttaatcagg gtctttcaca gttgacacaa gcaacaacac
     2461 tttctgacga gaaagctaag ggaattcaat ccttaattgt aggactacca gtcctaaatc
     2521 aaggcattca gcaactaaat acagagctat caacattgca accccctaac cttaatgctg
     2581 atgagttagg taatagctta ggagctatcg ctcaagctgc caaacaagtc attgctgaag
     2641 agactgccgc tcagaatgaa gaattatcgg ctctccaagc tactagcgtt taccaatcat
     2701 taactgctga acaacaagga gagttagctg cggccctcag tcaatctgat aaaagtcaga
     2761 ccgtatctgc agcccaaact attttaagtt ctgttcaaac tttgtcaaca agtttacagt
     2821 ctctctctca agaagatcag tcaaaacagt tggagcaact taaggaagct gttgcacaga
     2881 ttgctaatca atccaatcaa gctttgccgg gagcaagttc tgctttaact gaattatcaa
     2941 cgggattagc aaaggtaaat ggtagcttaa atcaacaagt tctaccagga agtaatcaat
     3001 tgacaacagg attagcacaa ttaaacaggt ataatactgc cattggttct ggggtaataa
     3061 aactctcaga aggtgccaat gccttgtcat ccaagtccgg agaattacta gatggtagcc
     3121 atcaattatc agaaggtgct actaaactag ctgatggtag ttctcaattg agtcagggtg
     3181 gtcatcaatt aacgagcgga ttgactgaat tatcaacagg attgtcaacc ttaaatggtt
     3241 ccttagccaa agcctctcag cagttatcgc ttgtttctgt gactgataaa aatgctaaag
     3301 ctgtcgcaaa acctcttgtg ttaaatgaga aagacaaaga tggtgttaag acgaatggga
     3361 tcgggatggc accttatatg attgctgttt ctctaatggt tgtggccctt tcaaccaacg
     3421 tcatttttgc taattcttta tctggtcgtc cggtcaaaga taaatgggat tgggctaaac
     3481 aaaaatttgt tattaatggt tttatttcga ctatgggatc cattgttctc tacttagcta
     3541 ttcaattatt agggtttgaa gcccgttatg gtatggaaac cttaggattt attatgctaa
     3601 gtggttggac gtttatggct cttgtcacag ctttggtcgg ttgggatgat cgatatggct
     3661 cttttgcttc tttggtcatg ttattgcttc aggttggctc ttcaggtggc tcttacccca
     3721 ttgagttaag tggagcattc ttccaaaagt tacatccttt cttaccaatg acttatgtgg
     3781 tatctggttt acgacaaacc atttcattat caggtcatat tggagtagaa gtgaaagtct
     3841 taactggttt cttactggca tttatggtat tagcactact catttatcgt cccaagaaaa
     3901 cagtctaaat aaaataacct caaaaaaaaa acagcatcta aggcagttac actagatgct
     3961 gttttttgtc ttattttgac aataacttga caatttctgt caagtttttt attgtcttgt
     4021 taagattatt tgatagtaat gtctccctta ttgagattaa ccgttaaatt tccagtagct
     4081 gctttattct cagccacata agaaataaca tctgaagttg attccttacc aatcttagct
     4141 ggtgtcagct gtgagccaag ggagacttta ccattttcgg tccttgctct gtaggcgatg
     4201 gcctttgctt ttgtcaaggt tatgtcaaca tcaatatcac caccagtaat agtattagtc
     4261 cctttaaggg taagggcatg agcggtgaag atagcatcat tgtcatagta gtcgttctcc
     4321 tcttcttctt tgcttttaga aacagtgaag gtcacatttt caagggttgt tctctcggct
     4381 tctataccac cagggtgtaa tgattgaata ttgacagctt ttagagtggt atctttagta
     4441 gttagttgtc catttcctaa gctgatggtc ccattttcca cactagaatc agtcaggttt
     4501 gaaaaagaat agtcagcttt taactcgaga tttgttaact gacttttagt ggcttccaac
     4561 ataccagtga tagtagcttt cttgactttg gtatgtaaaa gagtaagatt gccatcgaat
     4621 tgcatatctt ggacagttag atttgacaaa gtggtatgtg ggacattact tgccttaact
     4681 tcattgaggc tagttttttc aggaacagta atggtaatag aacggtagtt cttctcttga
     4741 cgactattat tgagagtaaa acctaaaatt tcaattccac cagtgatgac aacgtcttta
     4801 ggtgtttgcg aaagagatag ggtcttacct gttactgttg tgacgatagg atcaataagt
     4861 ttaggatgtg tgtaataggt cactgttggt ctttgaacgg gtcctgtctc tactgtaata
     4921 gttgaggcag aactgtcaat atcaagagca gttatttggt caaatgtttt gatgaccttt
     4981 ttgggagctg tttggtgttt aatgtcctga agtcctccag ttgcatagcc agtccctaga
     5041 agaatgcctc ctagaataat acataccaca ctggtcataa agcatttgcg tgagatattt
     5101 ttcatgcttg agctcctttc ttattactga tccacttaat aaaggcaaca aaagcccgtc
     5161 ctgaaaaacg aaggaatgct gtggcaataa tccaaataag aagaccacca ccaaaacaca
     5221 acaaggcgct gccgatgccc atagcttgtg caggaataga tgagccaatc aaggtcaagc
     5281 tttcaaacag ggtcactcct gcgacaagaa tacctgcaag cccaaaaatg taggcggcaa
     5341 agatagctgt gagcccgagc attaggcagg caataataaa gagaatcaac gccagcaata
     5401 cgggtaaagc aatcggagca gcaaagaggg acaaaatggt taatccaata attgttgccc
     5461 gatttttggg agttttctcg ggagttgcca agtgttttcc tagaacattt gaaatgattt
     5521 cactggcagc ttccttagga tttcctaaat cgtcgataac ctggctttct ttgtcaggac
     5581 cagcttcatc aaaatattca atataatatt ccatcgcttc atggtagtct tctctaggta
     5641 gtttgcgcaa gtatttatct agttctgcta gatactcagt ccttgtcatg tcttaagctc
     5701 ccttcaataa tcccgtctaa ggcaaattta tagctctgcc attctttttt tagaaaaatc
     5761 agctgtgctc tccccgaaga agtgacagca taatatttac gctttcgtcc ctgatgttct
     5821 tgactataag ttgtcaaaaa acctgctttc tccaattttt taagaatagg atagagagtg
     5881 gattctttga tattagccac tagtttaatg gtttgactaa tatcgtaacc gtaagaatca
     5941 tttttttcga caatggctaa aatcagaaat tcaatcaagg tagccgatac tggaaaatac
     6001 attttag
//
LOCUS       DAXWIQ010000069         5387 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000069 DAXWIQ010000000
VERSION     DAXWIQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.069"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(<1..1207)
                     /locus_tag="ACGEUI_001638"
     CDS             complement(<1..1207)
                     /locus_tag="ACGEUI_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFF8166456.1"
                     /translation="MKNNNKWIIAGLASFLFPLSIIFIILLSMGIYYNSDKTILASDA
                     FHQYVIFAQNFRNIMHGSDSFFYTFTSGLGINFYALMCYYLGSFFSPLLFFFNLTSMP
                     DAIYLFTLIKFGLIGLAACYSFHRLYPKISAFLMISISVFYSLMSFLTSQMELNSWLD
                     VFILLPLVILGLNKLITENKTRTYYLSISLLFIQNYYFGYMIALFCILYALVCLLRLN
                     DFNKMFIAFVRFTAVSICAALTSALVILPTYLDLSTYGENLSPIKQLVTNNAWFLDIP
                     AKLSIGVYDTTKFNALPMIYVGLFPLMLSVIYFTLESIPLKIKLANACLLTFIIISFY
                     LQPLDLFWQGMHSPNMFLHRYAWSFSIVILLLACETLSRLKEVTQIKAGFAFIFLIIL
                     TSLPYSFSQQ"
     gene            complement(1290..2909)
                     /locus_tag="ACGEUI_001639"
     CDS             complement(1290..2909)
                     /locus_tag="ACGEUI_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8166457.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEEERAIDVVIMGNEQLYNIMKEKDAI
                     YMKADFSEEDGVRAAELEGIFAELGGWEAESEASQLLQNLNIPEDLHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSISWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKQSSELAARLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREMGNDFLTVENLSVTIDGEKI
                     IDNISFILRPGDKAAIIGQNDIQTTALMRALADDIDYEGTIKWGVTTSRSYLPKDNSK
                     DFATEESILEWLRQFASKGEDDDTFLRGFLGRMLFSGDEVKKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLILDDPTNHLDLESISSLNDGIKDFKESVIFASHDHEFIQTIANHIVVI
                     SKNGVIDRIDETYDEFLDNPEVQARVAELWK"
     gene            complement(2988..3860)
                     /locus_tag="ACGEUI_001640"
     CDS             complement(2988..3860)
                     /locus_tag="ACGEUI_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8166458.1"
                     /translation="MKKRGIDLLLVTIGSFITAIGFNTMFVDNHIASGGMVGIAVVIK
                     ALFGISPSLFLMASNIPLLLMCYFFLRKQNFIKTLYGSWIYPIAIRSTNSLPTLTHNQ
                     LLAAIFGGIICGIGLGMVFWGNSSTGGTGILTQILHKYSPLSLGVAMTIVDGISVLMG
                     FIALSADDVMYSTIGLFVIGYVISVMENGFDSSKNVMIISKDYQAIREYITTVMDRGV
                     TKLPIRGGYTTSDKIMLMAIVSSHELPTLQEKILEIDDTAFIVVMPAAQVMGRGFSLT
                     KQYKREDKDVLLPM"
     misc_binding    3983..4224
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4279..5301
                     /gene="trpS"
                     /locus_tag="ACGEUI_001641"
     CDS             4279..5301
                     /gene="trpS"
                     /locus_tag="ACGEUI_001641"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFF8166459.1"
                     /translation="MTKPIILTGDRPTGKLHLGHYVGSLKNRVFLQNENKYKMFVFLA
                     DQQALTDHAKESELIQESIGNVALDYLSVGLDPKQSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPSGFLVYPVSQAADITAFKANLVPVGNDQKPMIE
                     QTREIVRSFNHTYHTDCLVEPEGIYPENEKAGRLPGLDGNAKMSKSLGNGIYLSDDAD
                     TVRKKVMSMYTDPNHIKIEDPGQIEGNMVFHYLDIFARKEDQADIEAMKEHYQIGGLG
                     DVKTKRYLLDILERELAPIRERRLEYAKDMGEVFRMLQEGSQKARTVAAKTLSEVKSA
                     MGINYF"
BASE COUNT         1876 a          906 c          981 g         1624 t
ORIGIN      
        1 attgttgaga aaagctataa ggaagagatg tcagtataat gaggaaaata aaagcaaaac
       61 ctgcttttat ttgagtcact tcttttagtc gagagagagt ctcacatgcg agtaatagga
      121 taactatgga aaaagaccaa gcgtagcgat gcaaaaacat atttggtgag tgcatcccct
      181 gccaaaaaag atcaagtggc tgtaggtaaa aacttattat aataaaagtt aacaagcagg
      241 cattggctaa ttttattttt aaagggatac tttctaaagt aaaataaata acactaagca
      301 ttaggggaaa taatcctacg taaatcatag gcagagcatt aaacttggta gtatcgtaca
      361 ctcctattga gagcttagca ggtatatcca aaaaccaagc attgttcgta actaactgtt
      421 ttatcgggga tagattctct ccataagttg acaaatctag ataggtagga agtattacta
      481 gagcacttgt taaagcagca catattgaca cagctgtaaa cctaacaaaa gcgataaaca
      541 ttttgttaaa atcattgaga cgtaaaagac aaactaaggc gtaaagaata caaaaaagag
      601 caatcatgta gccaaagtag taattttgaa tgaataataa tgatatcgaa agataataag
      661 ttctggtttt attttctgtg ataagtttat ttaatccaag tataacaagt ggaagaagaa
      721 tgaaaacatc taaccaagaa tttagttcca tttgacttgt caagaagctc attaagctat
      781 aaaaaactga gatggaaatc atcaagaaag cactgatttt tggatataat ctatgaaaag
      841 aatagcatgc agctaatcct attaacccaa attttatcaa ggtaaacaaa tagatagcat
      901 ctggcataga ggttaaatta aaaaagaaaa gtaatggaga aaagaaactg ccaagataat
      961 aacacattaa agcataaaaa tttatcccta gtccgcttgt aaaggtataa aaaaaactat
     1021 cagaaccgtg catgatgtta cgaaagttct gcgcaaaaat aacatactga tgaaaagcat
     1081 cactagctag aattgtttta tcactattat aataaatgcc catcgataga aggatgataa
     1141 atataatact aagagggaac aaaaaactag caagtccagc aattatccat ttattattat
     1201 ttttcataaa ttatatggtc tttcgtttac aaattttaaa aaagaggcta ggaagagatc
     1261 ttcttagcca cctcatttat cataaggttt tatttccaaa gttcagcaac tcgggcttgg
     1321 acttctggat tgtcaaggaa ttcgtcataa gtttcatcta tacggtcgat aacaccattt
     1381 tttgaaataa caacgatgtg gttagcaatc gtttgaataa attcatggtc atgactggca
     1441 aagataacag attctttgaa gtctttgatt ccatcattaa gacttgaaat agattccaaa
     1501 tctaagtgat tagttgggtc atcaagaata agaacgtttg atttcaatag catcaactta
     1561 gaaagcatga cacgcacttt ttcgccaccg gaaagaacgt taacagattt tttaacttcg
     1621 tctccagaga aaagcatccg tcctaagaac ccacgtaaaa aggtatcatc atcttcacct
     1681 tttgaggcaa attgacgtaa ccattcaaga atagattctt ccgtggcaaa atcttttgaa
     1741 ttgtctttag gcaagtaaga acgacttgtt gtcactcccc atttaatggt tccttcgtag
     1801 tcaatatcat ctgctaaagc acgcataaga gcagttgtct gaatatcatt ttgaccaata
     1861 atagcagctt tgtcacctgg acgtaaaatg aaactgatat tatcaatgat tttctctcca
     1921 tcgatcgtta cagaaagatt ttcaacagtg aggaagtcat ttcccatttc acgctctgct
     1981 ttaaaattaa taaatgggta tttacggctt gagggaacaa tttcttctaa ttcaatctta
     2041 tcaagcattt ttttacgaga ggttgcttgt tttgatttag atgcgtttgc tgagaaacgg
     2101 gcaacgaatt cttgtaattc tttgattttc tcttctgctt tagcattgcg gtcagcttgt
     2161 aaacgagcgg ccagctcaga agattgtttc cagaaatcgt agttacccac aaagagtttg
     2221 attttaccaa agtcaaggtc ggccatgtga gtacatactt tattcaagaa gtgacggtca
     2281 tgggaaacaa cgatgactgt attttcaaaa tcaatcaaga aatcttccaa ccatgaaata
     2341 gattgaatat caagaccatt ggttggctcg tcaagtaata aaacgtcagg cttaccaaag
     2401 agcgctttag caagaaggac tttcacttta tctccattag ccagttcact catattttgg
     2461 tagtgaaggt cttctggaat atttagattt tggagaagtt gagaagcttc actttctgcc
     2521 tcccagccac ctaattcagc aaagatacct tctagctcag cggcacgaac cccatcttct
     2581 tctgaaaaat ctgctttcat atagatagcg tctttttctt tcatgatatt gtagagttgt
     2641 tcattaccca tgattacaac atcaatcgca cgttcttctt cataatcaaa atgattttgg
     2701 cgaagaacag ataagcgttc gtcaggtcct aaagagatat gaccagtact tggttcaata
     2761 tctcctgcaa ggattttaag aaaagttgat tttccggcac cattggcacc gattaagccg
     2821 taagtattgc cggctgtgaa tttgatatta acatcatcaa atagttttcg atcactaaag
     2881 cgtagtgata catcagaaac tgtaagcaat ttgtcacctc attttattta tctaagcttc
     2941 attgtaccta aaatagtccc ctttttcaat cagaaaaggg ctaatcacta cataggcaat
     3001 aaaacgtctt tgtcttcacg tttgtattgt ttagtcaaac taaatcctct tcccataact
     3061 tgagctgctg gcatgacaac aataaaggct gtatcgtcaa tttcgaggat tttttcttgg
     3121 agagtaggta attcgtgact tgatacaata gccattaaca taattttatc agaagtagta
     3181 taaccaccgc gaataggaag tttagtcact ccacgatcca taacagttgt gatatattct
     3241 cgaatagctt gataatcttt ggaaataatc ataacatttt tggatgagtc aaagccattt
     3301 tccataacgc taatcacata accaattacg aaaagtccaa tcgtagaata catcacgtca
     3361 tctgcagaaa gtgcaataaa tcccataagg acactaatac catctacaat tgtcattgca
     3421 actccaagag ataaaggaga gtatttatga agtatttgtg ttagaattcc agttcctcct
     3481 gtcgaagagt ttccccaaaa aaccattcct aaaccaatgc cacagataat accaccaaaa
     3541 atagctgcta acaactgatt gtgtgtcaga gtaggtaatg aattggttga acggatggca
     3601 ataggatata tccaagaacc ataaagtgtt ttaataaaat tttgttttct aagaaagaag
     3661 taacacatta ataataaggg aatattacta gccattaaaa aaagcgacgg actaatgcca
     3721 aatagagctt ttataacaac tgcaatacca accatacccc ctgatgcaat atgattgtcg
     3781 acaaacattg tattgaaacc aatagctgtt atgaatgagc caattgtgac aagtagtaaa
     3841 tcaatccctc gttttttcat gcttttaatc tcctttataa ctggttaaaa atagtagact
     3901 cattatctct taaatgtact gagaccgtca agtcatattt tcaaaaaatc tggtgaactt
     3961 gacaagaagt ttaaaatttc atagaataaa tctaaattag aagagtattc ttttcatgtt
     4021 tttagagagt tagtggttgg tgcaagctaa caacagaaaa ggtgaaatgg actaatgatt
     4081 atttgaaagg aaaccataac tttcaggttg gcacccctta ccgtgcagct tctttcattg
     4141 agaagattga gatagtatct cgttttgata aagcagatgc taattgaggt ggtaccgcgt
     4201 attacttgta ataacgccct cacgttttaa tagcgtgggg actttttgct atatcaagag
     4261 atagttaggg agaaaatgat gacaaaacct attattttaa ctggagatag accaactgga
     4321 aaattacatt taggtcatta tgtcggaagt cttaaaaatc gtgtcttttt acaaaatgaa
     4381 aacaagtata agatgtttgt tttcttggcc gatcaacagg cactgacgga tcatgctaaa
     4441 gaatccgaat taattcaaga atctattgga aatgttgcct tggattacct ctctgtcggt
     4501 ttggatccga agcaatcaac gatttttatt caaagtcaga ttccagagct agctgaattg
     4561 agcatgtatt atatgaatct ggtatcatta gcacgtttgg aaagaaatcc cactgttaaa
     4621 accgaaattg ctcagaaagg ttttggcgaa agtattccat ctggtttttt ggtttatcca
     4681 gtatcacagg ccgctgatat tacagcattt aaagcgaatt tagtacctgt aggtaacgac
     4741 cagaaaccga tgattgaaca aacacgtgaa attgtgagaa gttttaatca tacttaccat
     4801 acagactgtt tagtagaacc tgaaggtatt tatccagaaa atgaaaaagc tggacgctta
     4861 cctggtcttg atggcaatgc caaaatgtct aagtcattgg gaaatggaat ctatctctca
     4921 gatgatgcag ataccgttcg caaaaaagtg atgagcatgt atactgatcc aaatcatatt
     4981 aaaatagaag atcctggtca aattgaaggg aatatggtct ttcattattt ggatattttt
     5041 gctagaaaag aagatcaagc tgatatcgaa gcaatgaaag agcattatca aataggtggt
     5101 ttaggagatg tgaaaacgaa acgctacctt ttagatattt tagaacgtga attagcacct
     5161 attcgtgaaa gacgtttgga gtacgctaaa gatatgggag aggtgttccg tatgttacaa
     5221 gaaggtagtc aaaaagcaag aactgtggca gccaagactt tatcagaagt gaagtcagca
     5281 atgggtatta attattttta atgttttaga gaaaaagcta agttagtaat gataagataa
     5341 ttactattat caaaacagga cctaatttgt catttcttta caggata
//
LOCUS       DAXWIQ010000070         5382 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000070 DAXWIQ010000000
VERSION     DAXWIQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.070"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(260..574)
                     /locus_tag="ACGEUI_001642"
     CDS             complement(260..574)
                     /locus_tag="ACGEUI_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166460.1"
                     /translation="MPRLISQQILSEYVKSLEVEADKVVIPGQNTPVFSWDRDGNGNL
                     SIDTPLLKVRGESLATQADLTTISLTPGPKGELWQTSVERLDIKVDKHISLTFKNGVR
                     ID"
     gene            complement(709..1092)
                     /locus_tag="ACGEUI_001643"
                     /pseudo
     CDS             complement(709..1092)
                     /locus_tag="ACGEUI_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1197..1469)
                     /locus_tag="ACGEUI_001644"
     CDS             complement(1197..1469)
                     /locus_tag="ACGEUI_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166461.1"
                     /translation="MELVKTIQIGDDIYPPIPDLFGIQENQEFNLYKSTDGTLVLSPS
                     DSKLSADHQSISSDEQMTTAVEQARLDEIATSVLSRHLDAFKELTE"
     gene            complement(1488..1793)
                     /locus_tag="ACGEUI_001645"
     CDS             complement(1488..1793)
                     /locus_tag="ACGEUI_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8166462.1"
                     /translation="MKEYQVTMSDDAKADLLSIYHYVRDELCAPQAADDLLERLSQGM
                     LSLSIFPERCAIIEDLIGKGYTFRQLIVKKYRIVYHVLEDEVIIVAVVYGSRHMNNW"
     gene            complement(1793..2095)
                     /locus_tag="ACGEUI_001646"
     CDS             complement(1793..2095)
                     /locus_tag="ACGEUI_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFF8166463.1"
                     /translation="MAKTGTLNLRVDDSVKSAADDILKRLGIPMSTAIDMFLNQIILT
                     GGIPFDVSLPEAPQRVNVDYMSQEKFYDKLITSFEDAKTGNRQDVREFLFQLKENA"
     gene            complement(2150..2308)
                     /locus_tag="ACGEUI_001647"
     CDS             complement(2150..2308)
                     /locus_tag="ACGEUI_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFF8166464.1"
                     /translation="MRQAPGGNIKPDKEKSVEKIDGIVGLILGLDRCICRQGDEASVY
                     DERGILSF"
     gene            2342..3235
                     /locus_tag="ACGEUI_001648"
     CDS             2342..3235
                     /locus_tag="ACGEUI_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFF8166465.1"
                     /translation="MTNMLDFPYHHEFIEFLGRWTHILIALPKRHHGQVDTAIPRYLL
                     VTIREKTGIVKLTTVQNTAFTTQSNQLKKQYGQFLLTESNNCCACPGCDRPLILTSGN
                     LAAENYEVSAIEKDKDAEALNLIALCPDGFLTYQADRRKKIVTTLKNVKKILVSAHNS
                     QQAISDMKLDSGIVSVLTSLNKLKCDEYNISYDSKQLTDKISPENNRTLYQLVKNQVI
                     DNYLTIQKIIVNLDKQGKIDYEKIQYQTRSMYKKLKAAKHNNLAIFNTISEKLHLATL
                     QDIYFCQMIVSYFIQKCEVLE"
     gene            3235..3459
                     /locus_tag="ACGEUI_001649"
     CDS             3235..3459
                     /locus_tag="ACGEUI_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 7"
                     /protein_id="HFF8166466.1"
                     /translation="MQLPNKLYSYQKSTLAYLPRVLNEIKSRNSNVKDIFHAISEELD
                     DPTDFLSIMDCLYALNAIEMSNEGEVKLCL"
     gene            3450..5108
                     /locus_tag="ACGEUI_001650"
                     /pseudo
     CDS             3450..5108
                     /locus_tag="ACGEUI_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904398.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
     gene            5098..5307
                     /locus_tag="ACGEUI_001651"
     CDS             5098..5307
                     /locus_tag="ACGEUI_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8166467.1"
                     /translation="MRFSYNKLWKLLIDKGWTKTKLRQEAGISSSTIAKLGKGENITT
                     DILLEICIALDCKIEDIVEIVDNNI"
BASE COUNT         1753 a         1129 c          915 g         1585 t
ORIGIN      
        1 taaaattacc ctagagtatt aaaattccca ctcaaactcc tagtcccttt gtttcaagct
       61 atttagaact tttattgtat tatccacttt attccgattt ctgttgctat tagttttatc
      121 atacaatcta acctttaccc tttttaggga gtgcaaaatt caaatgaatc gtgcatctca
      181 actcccaata taacttcttt cattatacca cagataaact aaaaaaggac gtgccgaatt
      241 gatacgccct tgccttttat caatcaatcc gtacgccatt tttgaaggtt aggctgatgt
      301 gcttgtctac ttttatatcc aacctttcaa cacttgtctg ccagagttct ccctttggac
      361 caggagttaa agagatggtt gtaaggtcag cttgtgtcgc tagactttcc cctctcacct
      421 ttaggagcgg tgtgtcaatg gaaagattcc cattcccatc cctatcccaa ctaaagactg
      481 gagtattctg tcctggaata acgaccttat cagcttcaac ttctaggctc ttcacatact
      541 ctgatagaat ctgttgggag ataagccttg gcatccatgc tgaactagag acggtcaact
      601 catcaatctt agctttgtga agtgctgcaa ttcgtgcttc taaggcatcc gttgccagat
      661 aatcaagagc agctttctta tcagaacgct cgccttctgc tttagcagta gcgataccat
      721 accactttta tctcgtataa tctgtacata ggcatttccc catatcaaaa gatggctcat
      781 gattgtctct cgaaagataa aggaggacat ctctggattg ggttcatcat gaagaagaaa
      841 gtacagtggg tggtccagct ttttctcctt cccattttct gtccgttcat agatgtgaat
      901 aggaagtgac gccaccgctt cagcaagaac acgcacacag gcatagactg ccgtcgtctg
      961 catagtctta aactcatcca cattctcgcc actagtcgtg cgaccaaaga ggtaggaaaa
     1021 gtcctgacct tcataactgt tttggggctt atctctagcc ctttttcttc caattaaatc
     1081 aagtagtccc ataagccctc cttattttgg ggtacaaaaa aagcacctcc tttgaagtgc
     1141 tttcggtata tttctttcaa agtaatcaac agtctttctt acaaaaccta gtattctcat
     1201 tctgtcaatt ccttaaatgc gtcaagatgt cgtgataata ctgaagtcgc aatctcatct
     1261 aaccttgctt gttcaacagc tgttgtcatc tgctcatcac tgcttatact ttggtgatcc
     1321 gctgatagtt tgctatcaga tggacttaac accaaagttc catcagttga tttataaagg
     1381 ttaaactcct gattctcttg aatgccaaat aggtcaggta tcggaggata gatgtcatca
     1441 ccaatttgta tcgtctttac gagttccata catcactctc ccttacttta ccaattattc
     1501 atatgacgtg atccatagac gaccgcaaca attataactt catcttccag aacatgatat
     1561 acaatgcgat attttttgac aatcagttgt ctgaaggtat agccttttcc aatcagatcc
     1621 tcaatgatgg cacaacgttc aggaaaaatg gatagtgata acattccttg ggataacctc
     1681 tcaagaagat catccgctgc ctgtggtgca cagagttcat cacgaacata gtgatagatg
     1741 ctcagcaaat ctgctttagc atcatccgac atggtgacct gatattcttt cattaagcat
     1801 tttccttcaa ttgaaataga aattctctga catcctgacg gttgccagtt ttcgcatctt
     1861 caaagctggt gataagctta tcataaaact tctcctgact catgtaatca acattaactc
     1921 gttgaggcgc ttcaggtaga gaaacatcaa atgggatacc cccagtcaaa ataatctgat
     1981 tcaaaaacat atcaatcgca gttgacatag gaatacctaa gcgtttcaaa atatcatctg
     2041 ccgcactttt tactgaatca tcaactcgta aattcaaagt tcccgtttta gccatggtta
     2101 tcctcctttt ttgtaacgac attgtatcac gtttttagag ttgactcaac taaaaactca
     2161 atatccctcg ctcatcatag acgctagctt catcaccttg gcggcaaatg caacggtcaa
     2221 gccccaggat aagacctaca ataccgtcaa tcttctcaac agatttttcc ttatctggct
     2281 tgatgttgcc tccaggggct tgtcgcatca ccacgttttg tcccatccat ttaaggactg
     2341 gatgaccaac atgttagatt ttccctacca tcatgagttt atagagttcc ttggacggtg
     2401 gactcatatc cttatagccc tgcccaaaag gcaccatggt caagtcgaca ccgcaattcc
     2461 aaggtatctt ctggtaacca tcagagagaa gacagggatt gtaaaattaa ccactgtgca
     2521 aaatacagcg ttcactaccc agtcgaatca actcaaaaaa caatacgggc agttcttgtt
     2581 aacagaatct aataactgtt gtgcatgccc aggctgtgat agaccactca ttctaaccag
     2641 tggcaactta gcagcagaaa attacgaggt ttcagccatt gaaaaagata aggacgctga
     2701 agcactgaat ctgatagccc tctgcccaga tggtttttta acctatcaag cagataggcg
     2761 caaaaaaatt gtaacaactc taaaaaatgt taaaaagatt ctagtctctg cacataacag
     2821 ccaacaagcg atttctgata tgaagttaga cagtggaatt gtttcagttt tgactagtct
     2881 caataaacta aaatgtgatg aatataatat ctcctatgac tcgaagcaat tgactgataa
     2941 gatatcacct gagaacaatc gtacgcttta tcaattggtt aaaaaccaag ttattgataa
     3001 ctacttaacc attcaaaaaa tcatcgtaaa tttggacaaa caaggaaaaa ttgattacga
     3061 gaaaatccaa tatcaaacgc gatcgatgta taaaaagtta aaagcagcca agcataataa
     3121 tctggctatt ttcaatacta tttcggaaaa actccatctg gctacactac aggacatcta
     3181 cttctgtcag atgattgtct cttattttat ccaaaaatgc gaggtacttg aataatgcaa
     3241 ttaccaaata aactttactc ctatcaaaaa agcacccttg cttatttgcc tagagtgcta
     3301 aatgaaatca aaagtagaaa ctcaaacgtc aaagatatct ttcacgctat ctcagaagag
     3361 ttggatgatc ctacagattt tctatccata atggattgct tgtatgcatt gaacgctatt
     3421 gaaatgtcta atgaaggaga agtaaaatta tgcttataga aatgtggtct ccagtattta
     3481 agaaacacgg agaaattcgg gaaccaatct gcttccacca tgggttaaac gtgattatgg
     3541 ggatggattt agctgataac tctatcggca aatcctcctc actattagcc attgatttca
     3601 tctttggagg taatagctat ctaaaatcaa ttgcggttaa aaaattaggt gatcatccta
     3661 tctacttctg ctttcaattt gagaaaaagt tttatttttc aagagatacg gccaacccag
     3721 atatcattct agtgtgtgac aagagctact ctccaaccgg tgaaacaatg gacctaggaa
     3781 tttttcttaa caaacttaag aaacgatatc atttagattc tccagagcta tcattccgat
     3841 tagctatgag tggcttcttt aggattgcag gaaaaagtaa tcaaaatacc gatttcccct
     3901 tacaagttta ttcgagccaa aaatcttctg aatctattac tactctcatc caactgttta
     3961 atttgtatga taacattgca cgatacaaag aacgtctgaa agataagagc gaccagttaa
     4021 caacttttcg taatgctcgc aggtatgcct tcatctccaa cctggttggc ggtaaaaagc
     4081 aatttgaagc aaatgtctca gaaataaaaa gattggaata tgatttagca catctccaag
     4141 atactcatca agataaaatt gactctgatg atatttaaaa aaatcaacaa aaactccaac
     4201 ttagaaatac taagctggag ttagaaagca gtcttcggga caagcaaaga cgtcttaagc
     4261 tattggacat cagtatcgaa tttggcttat atcctactga atctgactta acagagcttc
     4321 aacaatactt cccagatact aatctcaaaa aattatatga agttgaagct taccacaaaa
     4381 aattggaaac tattttagat tccgaattct caactgaacg tgaatcccta atagctgaga
     4441 tcgacgaatt agagagtcaa ttaacaactc taaaccaaga acttcaagaa ctaggaaaca
     4501 tcccaaatct ttcaagtgag tacttagaaa actactctaa actaacagct acaatcaatg
     4561 cactaaaaga acaaaacgag gcctacttaa aggagtctgg tctatccaaa gaaaaatcag
     4621 aaacggacgc tgacttgaaa cgcagtacag aagatatcct tctagatcta gaagtaaaaa
     4681 tcaacagtaa gatgcgtgaa gtcaataaca ttctatatcc agatattcgc aaagctcctc
     4741 aaattaacct aaatgctcac aatagctact ccttctatac accagatgat gatggtacag
     4801 gaactaagtt taaagggatg attgtattcg acttagtaat gctctacctt actaatctgc
     4861 cagcactcgc acacgattca ttactcttga gtaatgttag ctaccaagcg actgaagcac
     4921 ttttgaagct atatgaccag tcaaggtcat tgaacaaaca agtcttttta gtcttcgata
     4981 aagccagctc ttattctcca gatgctaatc aactcttgtc agaaaatacg gtattgagac
     5041 tctctagtaa cggaaatgaa ctctacggta tttcatggaa caaaggagaa aactcagatg
     5101 agatttagtt acaataaatt atggaaactt cttattgata aaggttggac caaaacaaag
     5161 ctcaggcaag aagctgggat tagttcttcc acgatcgcaa aattgggaaa aggggaaaat
     5221 atcactactg atattttatt ggaaatttgt attgcacttg attgtaagat tgaagatata
     5281 gtagaaattg tagataataa tatctagaaa taaaaaaagc actaagttga taagatgtgt
     5341 aagtcagatc aacttagtgc ctttactttg aaaaaaaata at
//
LOCUS       DAXWIQ010000071         5140 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000071 DAXWIQ010000000
VERSION     DAXWIQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.071"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..614
                     /locus_tag="ACGEUI_001652"
     CDS             <1..614
                     /locus_tag="ACGEUI_001652"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166468.1"
                     /translation="LEKIMDLLKNTNFLIPLISAIVSVSSIFISNWLGYRSQIRKLKF
                     DEEKEIYLTLYVPLIKWMNSQSFNNKSYYWLVAFPRYTTNAQDFLTGLLLKNFEKLPV
                     SVAMRYSEYTLNSATSLHFYRNTEYDYDYETFAKKASELFDLIIEQLLTEGTILSQKL
                     SLPNLSKSTLENFLADKKNYIGPRFLSLETHNKPLRPERPLPF"
     gene            complement(644..802)
                     /locus_tag="ACGEUI_001653"
     CDS             complement(644..802)
                     /locus_tag="ACGEUI_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166469.1"
                     /translation="MNWKKLMLGNLEHTFTSHDGKEKTSIEFEGGLLPALLVLGGITW
                     LAAWFITK"
     gene            1159..1983
                     /locus_tag="ACGEUI_001654"
     CDS             1159..1983
                     /locus_tag="ACGEUI_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFF8166470.1"
                     /translation="MKLGEIIKNFREEKKLSMDRFAEKSGLTKGYISMLEKNEHPKSK
                     KPIIPTEETLLKVAKGMGVDIDFVLSKLDSDQEIQINISPKNMLNMDNPSTPTTPKVE
                     LIPSTLQKINSTSSQLEHSRQIIVLDTAETLLEQQKEIKNNEDTIAELFSYNYYDHAA
                     SAGTGQYLNDVQVEKIELPVDYDADFVIPVYGDSMEPKYHSGDYVFVKLSVELTDGDI
                     GVFEYYGDAYIKQLLINDEGAFLHSLNSKYEDIPIDRDSDFRIIGEVVGSYSGNHS"
     gene            2019..2912
                     /locus_tag="ACGEUI_001655"
     CDS             2019..2912
                     /locus_tag="ACGEUI_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HFF8166471.1"
                     /translation="MENEVLHATHEGKLVLDNTTLDVAVLNNGQRIITQAGVFKALDR
                     PSRGNTRVIGIPTFMDAKNLQPLISKDLRGVINKIEYRDLNGRIQLGFDANILPLVSD
                     LYLKAREIGAIKSEAQMRTAQKAEMLVRSLAKVGVTALVDEATGYQYERERFELQKIF
                     KAYISEELLKWQKMFPDDFYMEIFRLNGWSYDVQSIKRRPGVVGKWTNELIYKRLPDG
                     VLEELKRRTPKTKKGQYKARFFQTLTPDIGHPDLNAQIYKVLGIMRISSNWKDFKEKF
                     NVMTSREDGIIELDLEFDEDK"
     gene            3033..4121
                     /locus_tag="ACGEUI_001656"
     CDS             3033..4121
                     /locus_tag="ACGEUI_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8166472.1"
                     /translation="MKIKSYKKENGETAYKFLLYAGYVNGKRKYIRREGFKTKQAARE
                     TLISLQAELDKPKSSMTFGALTDQWLKEYEKTVQCSTYLKTERNINKHILPKLDKVKI
                     GDINPLLIQRLTEEWCNDLKYGGKILGLVRNILNLAVRYGYINNNPALPITPPKIKRK
                     RKMNNNFYTLDQLKQFLELVEKTDNIEKIALFRLLAFTGIRKGELLALTWDDLNGNTL
                     SINKAVTRTQVGLEIDVTKTKSSDRLISLDDETLEILLELHETFPTSTLMFQSESGGI
                     MTPSLPRKWLLQIIKGTDLPQITIHGFRHTHASLLFESGLSLKQVQHRLGHGDLQTTM
                     NVYTHITQSAIDDIGTKFNQFVTNKQLD"
     gene            4484..5104
                     /locus_tag="ACGEUI_001657"
     CDS             4484..5104
                     /locus_tag="ACGEUI_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HFF8166473.1"
                     /translation="MTKKHLLTLLLISFFTSFLVACSTTKDKEPQPSDSEIITPRLHQ
                     AAHQDKRANFEKIKLATVDSSFTGGTSLEELISLFGEPSQHDPKTAGEVTIDAYTWQF
                     DQVTLTVNLYQNSSIVKTISNFTFARELGLSQKEYQQLQKGMSYEDVKKILTEPDNYS
                     QASSSDHQTLQAIWVSGLKTDTSGANISLVFENNQLTEMSQVGLEE"
BASE COUNT         1832 a          881 c          880 g         1547 t
ORIGIN      
        1 gattggagaa aatcatggat ttattgaaaa ataccaattt tttaattccg ttaatttcag
       61 caatagtctc tgtttcttct atttttataa gcaattggct tggttataga agtcaaatca
      121 ggaaattaaa atttgatgaa gaaaaagaaa tatacctaac cctctatgta ccgttaataa
      181 agtggatgaa ttcgcaatca ttcaataaca aaagttatta ttggctagtg gcgttcccta
      241 gatatacaac aaatgcacaa gattttctga caggcttgtt gttaaaaaat tttgaaaaat
      301 taccagtatc agttgctatg agatactctg aatacaccct aaattctgca acctctttac
      361 atttttatcg taacaccgaa tacgattatg attatgaaac atttgccaaa aaagcatctg
      421 aattatttga tctcattatt gaacaattgc taacagaagg gacaatatta tcccaaaagc
      481 taagcttacc aaacttatcc aaatccactt tagagaactt tttggcggac aagaaaaact
      541 atatcggtcc cagattttta tcactagaaa cccataacaa acccctaaga cctgaaagac
      601 cactgccatt ttaactcccc cctttccgcc ccttgtgggc tttttatttt gtaataaacc
      661 aagctgctaa ccaagtgata ccacctaaca ccaacagcgc tggcaataag ccaccttcaa
      721 attcgatgct tgttttttct ttgccatcat gactagtaaa cgtgtgttct aagttgccaa
      781 gcattagctt tttccaattc atgcaacctc tcctttcatt cttgcggaga tacagccaat
      841 gtgctaaact aaacttaccc cgttaggggg agaggacttc ttagccctct aattatcctc
      901 accactctat tgagtagtga atcttaagct taaaccaaag aatcttgatt tcgacttcta
      961 gttctttgcg tttaggcttt ttgtttagcc tagatttcat cagctgtacc tcctttcgtt
     1021 ttgcttaatc ccttaagctt gattatagtt taacacgtta aacattcatt gtcaagcgtg
     1081 ttaaacaaaa atattgaatt attttttgtt gtgttgtata atgtattaaa caatcattct
     1141 aggaagaggt ttttaacatt gaaattaggg gaaataataa aaaatttcag ggaagaaaaa
     1201 aagttatcaa tggataggtt tgctgaaaaa tctggtctta ctaaggggta tatttcaatg
     1261 cttgaaaaaa acgagcatcc gaaatctaaa aaaccaatta tccctacaga agaaactttg
     1321 ttaaaagtag caaaagggat gggggttgat attgattttg ttttgagtaa attagattcc
     1381 gatcaagaaa tacagattaa tatttctcct aagaatatgt taaatatgga taatccctcc
     1441 actcccacca ccccaaaagt cgaacttatc ccatctactc tacaaaaaat aaactctact
     1501 tcttctcaac tagaacacag tagacagata attgttttag atacagctga gactttattg
     1561 gaacaacaga aagaaattaa aaacaacgaa gatactattg ccgaattatt ttcttacaac
     1621 tactacgacc acgcagcttc agctggtaca ggtcagtatc taaatgatgt acaagtagaa
     1681 aaaattgagt taccagtcga ttatgacgca gactttgtta tccctgttta tggtgattcc
     1741 atggaaccga agtatcactc tggggattat gtatttgtta agctatccgt agagcttaca
     1801 gatggcgata taggcgtctt tgaatactat ggtgacgctt atatcaaaca gctacttata
     1861 aatgacgagg gggcatttct gcacagttta aatagcaagt atgaagatat accgatagat
     1921 agagatagtg attttaggat tattggtgaa gttgttggaa gttattcggg aaatcatagc
     1981 taacgaagct acggttgtga atgagtttag gagaaaatat ggaaaatgaa gttttacatg
     2041 caactcacga gggtaaatta gtcttagata atacaactct tgatgtagca gttttaaata
     2101 acggtcagcg aatcatcacc caagctggtg tttttaaagc attggacaga cctagtagag
     2161 gcaatacccg tgtgatcgga atacctactt ttatggatgc aaaaaacctt caaccattga
     2221 tatcaaagga tttaaggggt gtgatcaaca aaattgaata tagggattta aatggtagga
     2281 ttcaattagg ttttgatgct aacattttac cgctagtatc agatttatat ttaaaagcta
     2341 gagaaattgg agctatcaaa tcagaagctc agatgagaac tgcacaaaaa gcggaaatgt
     2401 tagttcgctc acttgcgaaa gttggtgtta ctgctttagt agatgaagct actggttatc
     2461 aatatgagag agaacgtttt gagcttcaaa aaatatttaa agcatatatt agtgaagaat
     2521 tgctaaaatg gcaaaagatg tttccagatg atttttatat ggaaattttt agattgaacg
     2581 ggtggagcta cgatgttcaa tcaattaaaa ggcgtccagg tgttgttggt aaatggacaa
     2641 acgaattgat ttataaaaga ctgcctgatg gagtgttaga agaattaaaa agaagaactc
     2701 caaaaacaaa gaaaggtcaa tataaagcta gatttttcca aactttaaca ccagatatag
     2761 gacatcctga tttaaacgct caaatttata aagttcttgg gattatgaga atttcatcta
     2821 actggaaaga tttcaaagag aaatttaatg ttatgacatc tagagaagac ggtataattg
     2881 aattagattt agaatttgat gaagacaaat aaaaaaagcc ccacgctctc aaagtttggc
     2941 gactctgagc gtgaggcaag acagtataag aaacaaccat taaaaaggtc gttttcttgt
     3001 acccatttta acaaattgaa agatggtatg caatgaaaat taaatcatat aaaaaggaaa
     3061 atggtgaaac tgcttataaa tttcttttgt atgccggtta tgttaatgga aaaagaaaat
     3121 atattaggcg agaaggtttc aaaactaagc aggctgcaag ggaaacctta attagtttac
     3181 aagctgaact tgataaacct aaatcaagta tgacatttgg agcattgaca gatcaatggc
     3241 taaaggaata tgaaaaaacc gttcagtgca gtacctactt aaaaacagaa agaaatatta
     3301 ataaacatat tttgccaaaa cttgataaag tgaagattgg agacatcaac ccactactta
     3361 tccagcggct tactgaagaa tggtgcaacg atttaaaata tggaggaaaa attcttgggc
     3421 ttgttaggaa tatcttaaat ctagctgtta gatacggata tatcaataac aatccagctt
     3481 tgccaattac acctccaaaa ataaaaagga aaagaaaaat gaataataat ttttatacac
     3541 ttgatcaact taaacaattc cttgaactag ttgaaaaaac tgacaacatt gaaaaaatag
     3601 ccttgtttag attattagca tttactggaa tacgaaaagg ggagcttctg gcactaactt
     3661 gggatgattt gaatggtaat actctatcaa ttaataaagc tgtcacacgt actcaagttg
     3721 gactagaaat agatgttacg aagacaaaat cgagcgatag attaatcagc ttagatgatg
     3781 aaactttgga aattttacta gaacttcatg aaacttttcc tacttctact cttatgttcc
     3841 aatctgaatc aggtggaatt atgacgccaa gtttaccacg aaaatggcta ttgcaaatta
     3901 tcaaagggac agacttacca caaatcacaa ttcatggttt caggcacact catgcaagct
     3961 tacttttcga atcaggtcta tccttgaaac aggtgcaaca tagattaggg catggagatt
     4021 tacagacaac tatgaacgta tatactcaca tcacgcaatc ggcaattgat gacattggaa
     4081 ctaaattcaa tcaatttgtt actaacaagc aactagattg acaactaatt ctcaacaaac
     4141 gttaatttaa caacattcaa gtaactccca ccagctccat caatgcttac cgtaagtaat
     4201 cataacttac taaaaccttg ttacatcaag gttttttctt tttgtcttgt tcatgagttt
     4261 ccgttatccc tatagccata ccatcacgct taccgatact ccatcaatac cactcaaaac
     4321 cttgtcatat cagatctttt tgaccatttt tttcatgaat atctaaaaaa aagaatcaaa
     4381 atagtactaa attccccata gcgcatgcgc tatggggaat catagctata tcttgtttat
     4441 aatttatgat ataatacagc aaaaatagtt taggaaataa catatgacaa aaaaacactt
     4501 actaacactt cttctcatct ctttttttac tagctttttg gtagcttgtt caacaacgaa
     4561 agataaagag cctcaaccgt ctgattcaga aatcattact ccccgactac accaagccgc
     4621 tcatcaagat aaacgcgcta actttgaaaa aattaaactt gcgactgttg attcctcatt
     4681 tacaggaggg acaagccttg aagaacttat ttcactcttt ggagagccta gccaacatga
     4741 tccaaaaaca gcaggcgaag taacaatcga cgcttatact tggcagtttg atcaagttac
     4801 tctcactgtt aatctttatc aaaatagtag tattgttaaa accatctcta attttacctt
     4861 tgcaagagag ttaggcttat cgcaaaagga ataccaacaa ttacaaaaag gaatgtctta
     4921 tgaagacgtt aaaaagatct taacagaacc tgataattat agccaagcgt catctagtga
     4981 tcatcaaact ttgcaagcga tttgggttag tggcttaaag acagatacaa gcggagctaa
     5041 tatttctctc gtttttgaaa ataatcagtt aacagaaatg tctcaggtag gacttgaaga
     5101 ataaaaaatg agtaatatat tttgctaaaa agattgattt
//
LOCUS       DAXWIQ010000072         5111 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000072 DAXWIQ010000000
VERSION     DAXWIQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.072"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            37..192
                     /locus_tag="ACGEUI_001658"
     CDS             37..192
                     /locus_tag="ACGEUI_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type A2 lanthipeptide"
                     /protein_id="HFF8166474.1"
                     /translation="MSFMKNSKDILTNAIEEVSEKELMEVAGGKKGSGWFATITDDCP
                     NSVFVCC"
     gene            275..1900
                     /locus_tag="ACGEUI_001659"
     CDS             275..1900
                     /locus_tag="ACGEUI_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HFF8166475.1"
                     /translation="MIKRNELKLEYQQHQFYEYFNSIFDYDILDTSIRETSEVLRKKT
                     HKLFYSEFENQLFETIMFLSMKTLVLDINHFSKEIENKSEAYEQYIQQIREENGINHF
                     FDRYPYLLKQINKEVGLIEESYSLLFDRFLEDLSEIKSCFNISEPLSNVAFSLGDSHS
                     KKQTVVKIAFKEKSVYYKPKSYHSHSILLELTSLLKSSNIPSFSLPKSLVKADYCWQL
                     GVAYTSSNKDEVAKIYFKYGVLAAFSEIFSITDLHMENVIVSGGDLYLIDVETFFQRK
                     LNVQNQNFEGITVDTYQRIYETSLSNGLFPVQFEKNSAPNVSGISGKGGKRKKGKYEL
                     INKNRGDMKLVKVDYFQEDGFNIPTLNGKVVEPLDYANEIISGFRECYIFLLSQRSKI
                     KEIVEGFPELKSRVPFRNTSDYGKFLQASTNPKYLFSEKKRKNLFSILYETKHIEHFI
                     VDNEIKDLMNGDIPYFSMDTRGNVYNSVGTLIGNLGDTTSLFDSITILNDERLKFTCE
                     LLEIVLKKPIKHWEREKGKSYQFLSISSEHYLL"
     gene            1956..2435
                     /locus_tag="ACGEUI_001660"
     CDS             1956..2435
                     /locus_tag="ACGEUI_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8166476.1"
                     /translation="MFDGDVMYNILLGRESVTEKQVIEICKRVSLYEDIRSMPMKFHT
                     PLFRDNPSLSGGQKQRISLARELVTTPRILVLDEPTSALDVKTERIIQKNVEALHCTR
                     VLVTHRLNTVEKADKILIMDNGKIIDYGNHHYLYKNNKDYCDLYDSYMNKYQEEEVK"
     gene            2432..3169
                     /locus_tag="ACGEUI_001661"
     CDS             2432..3169
                     /locus_tag="ACGEUI_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8166477.1"
                     /translation="MKQLVLKDVCKKYPNQLNYALDHINLIVEKGEFVAVMGRSGSGK
                     TTLLNVTSIIDKIDSGNIYCADKEISTFSDKEATNFRKNDIGFVFQDYMLLDSLTIRE
                     NISVALSLKNIDSSKIDDLINSYAKRFNLYEQLKKYPYQLSGGQRQRVSIIRAIIKEP
                     EIIFADEPTGALDLKSSEETMMILSEINKTEKVTILMVTHDVLSASYADRVILLKDGK
                     LHMEIDKKDCGESFYDVISQALSDRGE"
     gene            3171..5078
                     /locus_tag="ACGEUI_001662"
     CDS             3171..5078
                     /locus_tag="ACGEUI_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFF8166478.1"
                     /translation="MIWSITKSNIKKNFSLYRIYFLATIGLLSIFIAFLNFISDKIIT
                     EKIGDSGQALVIANGSLIFLIVFLVVFLIYFNNFFVKKRSQELGVLAILGFSKRELTK
                     LLTLENLVILVLSYLVSLLLGPTLYFLAVLAITHLLNLTMEVQWFITVNEIIESLGIL
                     VVVFLINVITNGLIISKQSLIEFVNFSRKAEKKIKIRKVRAIIAITALLLSYILCLAT
                     VFSSTRNMLLSIGMVPVSLLIIVLVVLGTVFTIRYGLAFVVSLLKENKKRLYRPLSNI
                     IYPKFNYRIATKNKLLTVLGGLLTVTVSVAGMMVMLYAYSLNGIERLTPSAIEYNVES
                     ENGQVNVTTILENDQVSLVDVGLLRLNTIPEVTITDSGQTIPYFDIINYSDYKELMKA
                     QGRTNSIEGSKSLPLLINYYPTEISLGKTFNLGNAYDVTVKQVSTNNVFSFSTSVTTL
                     VVSDKLYAKLSSRFPEKEMTIRTFNGTSIRSSEAFYNQFSMVPDVISSYSKEHTVKTA
                     NIATYIFITFLSILFIICTGSILYFTSLIEIMENKEEYGYLSKLGYSKKMIHRILRYE
                     TGILFLIPVFIGIVNGGMLLIYYKYLFMDTLVAGNIIMLSLLLCLLFFLIIYGTFYVL
                     TLRLVTSIIKN"
BASE COUNT         1788 a          680 c          929 g         1714 t
ORIGIN      
        1 gtagaaaata gtcactacat actgatagaa aggagaatga gttttatgaa aaattcaaag
       61 gatattttga ctaatgctat cgaagaagtt tctgaaaaag aacttatgga agtagctggt
      121 ggtaaaaaag gttccggttg gtttgcaact attactgatg actgtccgaa ctcagtattt
      181 gtttgttgtt aattaaaaca atctggacaa gcagactaaa aatttcctgt ttagactcag
      241 tctaggcagg atttttaagt agtggtagga aaaaatgata aaaagaaatg aattaaagtt
      301 agagtatcag caacatcagt tttacgaata ctttaactca atttttgact atgacatatt
      361 agatacttct attagagaaa ccagtgaagt gttaaggaaa aagacacata agttatttta
      421 tagtgagttt gaaaatcagt tatttgaaac gatcatgttc ctttcgatga aaacactagt
      481 tttagatata aatcattttt ccaaggaaat tgaaaataag tcagaggcat atgagcagta
      541 tatacagcag ataagagaag aaaacggaat aaatcatttt tttgacaggt atccgtattt
      601 gctaaaacaa attaataaag aagtaggact gatagaggaa tcatattctt tattatttga
      661 tagattttta gaagacttat ctgagataaa gtcgtgcttt aacattagtg agcccctaag
      721 caatgttgcc ttttcacttg gtgacagtca ctctaaaaag caaacagtcg ttaaaattgc
      781 gtttaaagag aaatctgttt actacaagcc taaaagttat cattcacaca gtatcttgtt
      841 agaacttact agtttattga aatctagtaa tataccctca ttcagtcttc ctaaaagctt
      901 agttaaagca gattattgtt ggcaacttgg tgtagcttat acaagttcca ataaagatga
      961 agtagcgaaa atttacttta agtatggtgt tttagcagca ttttctgaaa tattttctat
     1021 tacggactta catatggaaa atgtgattgt ttcaggcgga gatttatatt taattgacgt
     1081 tgaaacattt tttcaaagaa agttgaatgt acaaaatcag aattttgaag ggattactgt
     1141 agacacctat cagagaattt atgagacatc actttcaaac ggtttatttc ctgtacaatt
     1201 tgagaaaaac agcgctccta acgtttctgg aataagtgga aaaggtggaa aaagaaaaaa
     1261 aggaaaatat gaactgataa ataaaaatcg tggagatatg aagttagtaa aagttgacta
     1321 ttttcaagaa gatgggttca atattcccac attaaatggg aaagtggtag agcctcttga
     1381 ctatgctaat gagatcattt caggatttag agagtgctac atatttcttc tatcacaacg
     1441 ttcaaagatt aaagaaatag tagaaggttt tccagaattg aaaagcagag tcccatttcg
     1501 aaatacttcc gactatggaa aatttttgca agcttctacg aatccaaaat atcttttctc
     1561 agaaaaaaag aggaagaatt tattttcgat actatatgag acaaaacata ttgaacattt
     1621 tattgttgat aatgaaatta aagatttgat gaatggtgat attccttact tttcaatgga
     1681 taccagagga aacgtctata attctgtagg gacattaatt ggaaatctag gagatacaac
     1741 ctcgttattt gatagtataa ctattttgaa cgatgaaaga ttgaagttca cttgtgaatt
     1801 gcttgaaata gtcttgaaaa aaccgattaa gcattgggag agggaaaagg gcaaatctta
     1861 tcagttttta tcaatctcct cagaacatta ccttctctaa aattgataaa actgatttta
     1921 ggaatatatc ggcttttgta cctcaagatt ctccaatatt tgatggggat gtgatgtata
     1981 acattttgct aggaagagaa tctgttacag aaaaacaagt tattgaaatc tgtaaaaggg
     2041 tatcactata tgaggatatc aggagtatgc caatgaagtt tcatacccca ctttttcgag
     2101 acaatccatc actatctggg gggcaaaaac aacgaatttc tctagcaaga gagttagtaa
     2161 ctacccctag aatcttagtt cttgatgaac ctacatcagc tttagatgta aaaactgaaa
     2221 gaataatcca aaaaaatgtt gaggctttac attgtacgag ggttttggtt acccatagac
     2281 ttaatacagt tgaaaaagct gataagattt taataatgga taatggcaaa attattgact
     2341 atggaaacca tcattatttg tacaaaaata ataaggatta ttgtgactta tatgactcgt
     2401 atatgaataa atatcaggag gaagaggtaa aatgaaacaa ttagtattaa aagatgtttg
     2461 caaaaaatat cccaatcaac tgaattatgc tctagaccat atcaatttga ttgtggaaaa
     2521 aggagaattt gtagctgtca tggggcgtag cggtagtggg aaaacgacgc ttttaaatgt
     2581 tacttcaatt attgataaaa ttgatagtgg gaatatttat tgtgcggata aagagattag
     2641 cacattctct gataaggagg caactaattt tagaaaaaat gatattggtt ttgtttttca
     2701 agattatatg ctgttagata gtctaaccat tagggagaat atttctgttg ctttgtctct
     2761 aaaaaatatt gattcttcaa agattgatga tttgataaat agctatgcaa aaagatttaa
     2821 tttatatgaa cagttgaaga aatatccgta tcaactttct ggaggtcaaa gacagagggt
     2881 cagtattatc agagcaatca ttaaagaacc agagattatt tttgctgacg aaccaactgg
     2941 agcacttgac ttgaaatctt ctgaggaaac aatgatgatt ttgagtgaaa tcaataaaac
     3001 agaaaaggta actattttaa tggttactca tgacgtttta tcagcttctt atgctgacag
     3061 agttatactc ctcaaagatg ggaaacttca catggagatt gataaaaaag attgtggaga
     3121 gtcgttttac gatgtaatta gtcaagcttt atccgataga ggagagtaat atgatttggt
     3181 caattacaaa atctaacatt aaaaaaaatt tttcgttata tcgtatctat tttctagcta
     3241 cgattggttt attaagtatt tttatagctt ttctaaattt tatctcagat aaaatcatta
     3301 cagaaaaaat tggggatagt ggtcaagctc tagttatcgc taatgggtca ttgatttttt
     3361 tgattgtatt tttggtggta ttcttaattt acttcaataa tttctttgta aaaaaacgta
     3421 gtcaagagct tggagtctta gcaatactag ggttttcaaa aagagaatta acaaaattac
     3481 taactttaga aaatcttgtt attctagttc tgagttactt ggtaagttta ttgctgggac
     3541 cgactttata ttttttagct gtactggcaa ttactcatct attgaattta acaatggaag
     3601 ttcagtggtt tattacagtt aatgagatta tagagtcttt aggaatatta gtcgtagttt
     3661 ttctgattaa tgtcatcaca aatggactta tcattagtaa acagtctttg attgaatttg
     3721 ttaatttctc aagaaaggct gagaaaaaaa ttaagataag aaaagtcaga gctattattg
     3781 ctatcactgc attgctattg tcatatattt tatgtttggc gacagtgttt tcatccacac
     3841 gaaatatgct attaagcata gggatggtac cggtttctct attgataatt gtcttagttg
     3901 ttttaggaac agtgttcacc atcagatatg gattggcttt tgtagtttcg ttgttaaaag
     3961 aaaataaaaa aaggttatac cgtcctctgt ctaatatcat ctatcccaaa tttaactatc
     4021 gtattgcaac aaaaaataaa ttattaacag tcttgggagg tcttttaaca gtaaccgttt
     4081 cagttgccgg aatgatggta atgctctatg cttattctct taatgggata gagaggttga
     4141 ctccatctgc catagaatat aatgttgaat cagaaaacgg tcaagtcaat gttacaacta
     4201 ttttagagaa cgaccaagtg agcttggttg atgttggcct gttgcgattg aatactatcc
     4261 cagaggtgac tatcacagac tctgggcaaa caatacctta ttttgatata attaactaca
     4321 gtgactacaa agagttaatg aaagctcaag gcagaacaaa ttctattgaa ggtagtaagt
     4381 cactcccatt gttaataaat tattatccaa cagaaattag ccttggaaaa acctttaact
     4441 taggaaatgc atatgatgtt actgtaaaac aagtatcaac gaataatgtt tttagttttt
     4501 ctacaagtgt cacgaccctg gttgtttctg ataaattata tgctaaactt agttctcgtt
     4561 ttccagagaa agaaatgaca attaggactt ttaatggaac ttcgattagg tcaagtgaag
     4621 cattttacaa tcagtttagt atggttcctg atgttatcag tagttatagt aaggaacaca
     4681 cagtaaagac tgctaatatt gcgacttata tctttataac tttcctatcc atactcttta
     4741 ttatttgtac aggtagtatt ctgtacttta caagcctcat cgaaatcatg gaaaataaag
     4801 aagaatatgg ctatctaagt aagctaggtt atagtaaaaa aatgattcat cggattcttc
     4861 gatatgaaac aggtatactt ttccttattc ctgtattcat tgggattgta aatggtggta
     4921 tgttgcttat ttactataaa tatttattca tggatacatt ggtagcaggc aatatcataa
     4981 tgttatcttt attgctttgt ctgcttttct tcttgataat atatggcaca ttttatgtat
     5041 tgacattgcg gttagtgaca tccataatca aaaattaatt ttaaaaatct tttaacttta
     5101 acgattaagt a
//
LOCUS       DAXWIQ010000073         4643 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000073 DAXWIQ010000000
VERSION     DAXWIQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.073"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            32..421
                     /locus_tag="ACGEUI_001663"
     CDS             32..421
                     /locus_tag="ACGEUI_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HFF8166479.1"
                     /translation="MTKKELFENFQKNWMRLLSPFEIEDIDKWIDEDNMPVEVVNEAL
                     KETVIYNAPNLRYLNRVLNNWKRQGIDTVEKVEFARLQFENKKLSQNKNQQSNVPSWS
                     NPDYKEPDLKEFALGSMDGIEDGSGDF"
     gene            402..635
                     /locus_tag="ACGEUI_001664"
     CDS             402..635
                     /locus_tag="ACGEUI_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166480.1"
                     /translation="MDQEIFNFFNKQIKKDFGKTASKETFAKFASYCAEGIEKNGVKP
                     IFNWINLYAFGTGMTTAEADRLRIERYKQENVL"
     gene            632..772
                     /locus_tag="ACGEUI_001665"
     CDS             632..772
                     /locus_tag="ACGEUI_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166481.1"
                     /translation="MTKQHRETLIWYRASHQERERLLDFGLVDKSQYMTLLRQLRKKY
                     AI"
     gene            781..987
                     /locus_tag="ACGEUI_001666"
     CDS             781..987
                     /locus_tag="ACGEUI_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166482.1"
                     /translation="MTPEQAEKAKIRAKQELETFSIYLDQAIDELGGVLTSREVFLAA
                     GITYLGAGQTDIHAAVEGLCEQIQ"
     gene            1043..1372
                     /locus_tag="ACGEUI_001667"
     CDS             1043..1372
                     /locus_tag="ACGEUI_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166483.1"
                     /translation="MNIKEKIVVLRNTEDGSFLKSFKNKKDVLAYNVELTDSIQLASF
                     LPEEAYNIQKEKIDNLAETLGCDVVVIEASYDLKFIDGEDVPELTKEQKVKSMVNGMF
                     EQVFGGE"
     gene            1375..2304
                     /locus_tag="ACGEUI_001668"
     CDS             1375..2304
                     /locus_tag="ACGEUI_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFF8166484.1"
                     /translation="MANQLSTYTHKQFFNAPTIQKAFDDVWKGAGTQFAVSILSVLQG
                     SQSLKSASNESIYAAAMKAAVLNLPIEPSLGRAYLVPYKGQAQFQLGYKGLIELAQRS
                     GQYKNINAGIVYKSQLISYNPLFEELILDFSKPQDEIVGYFAAFKLLNGFEKVSFWTV
                     EKVTAHGKKFSKSFASGPWKTDFDAMAQKTILKDILSKYGPLSVEMQKAIEEDNQDST
                     ISTPKDITPQEANSLDDLIGHQNENKDAPNNLKDVTEDLHDEEEKTLADENKTALEDT
                     SYPADEIPDFDQETGEIKASEGNLFDNLGDLMP"
     gene            2301..3098
                     /locus_tag="ACGEUI_001669"
     CDS             2301..3098
                     /locus_tag="ACGEUI_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFF8166485.1"
                     /translation="MTKLDLLGKDYYSRESAIKYWSISQYKRFRECEARALAELRGGW
                     TDTRDNTALLVGNYVHSYFESKAAHEEFKAQNDSEMISTRGTTKGQLKKDYLVAEQMI
                     EALKNDSNFMAIYQGEKEAAITGFLGEVEFKGKIDCLNVERGYFVDIKTTKGPIDDTI
                     WNREERVRWFEAYGYILQMAAYKTMLEAKYNKPFKPIIYAVTKETPPDTRAIRIQNVD
                     AMQNELDDLAQNIKHLDDVKKGLEKPKPCGHCEYCRANQLTQRVMIF"
     gene            3108..3275
                     /locus_tag="ACGEUI_001670"
     CDS             3108..3275
                     /locus_tag="ACGEUI_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166486.1"
                     /translation="MTKYYVSAKIANLDIGAETEAENQHMAPIAFKETYDHLLRFGSH
                     KLIILGVEELN"
     gene            3453..3794
                     /locus_tag="ACGEUI_001671"
     CDS             3453..3794
                     /locus_tag="ACGEUI_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166487.1"
                     /translation="MEQIKITGTGTALILDRVNRIFAISGSLTMQWDFISGFKKIDDE
                     PSLDEDGELFETAYDLILAAKPKTKINLTSSYFAKEHKKDIDEIIKVFSFIEDNKRNI
                     FETLGIRGVLE"
     gene            3791..4303
                     /locus_tag="ACGEUI_001672"
     CDS             3791..4303
                     /locus_tag="ACGEUI_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166488.1"
                     /translation="MSNLVLSLDISTSGTGWALFKGSDLIQSGVLKHKSKSYFERGRY
                     MASQLRLIQSRALKKYDCYFSTIAVEKNSVMGPNQQSMLKIGIVTGIILGRLIADNVV
                     FINVSTWRKYWKFSYKDRSKKSMKLQSKIKVEQEFNKSVKDDEADAILIGSYYVNQGY
                     LDELETHDYY"
     gene            4387..>4643
                     /locus_tag="ACGEUI_001673"
     CDS             4387..>4643
                     /locus_tag="ACGEUI_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166489.1"
                     /translation="MTEEQMIDCLLYELVKKDKAIKKKSIIIAALTVMLIVVSGLCVS
                     LKSYYEPQIYGLRTQLSRTQKQLKRASEQNQRQTKRIADLT"
BASE COUNT         1705 a          779 c          993 g         1166 t
ORIGIN      
        1 taagatgatg ctgctagaag ggagtcgata aatgactaaa aaagaacttt ttgaaaattt
       61 tcagaagaat tggatgaggc ttctttcgcc gtttgaaata gaggatatcg ataaatggat
      121 agatgaagac aatatgcctg tagaagttgt taatgaagcg ttgaaagaaa cagttatata
      181 caacgcaccg aaccttagat acttaaacag agttctaaac aattggaagc ggcaagggat
      241 tgatacagtc gagaaagtcg aatttgctag attgcaattt gagaataaaa agctcagtca
      301 aaataaaaat caacaatcca acgtccctag ctggtccaat ccagactaca aagaaccaga
      361 tttaaaagaa tttgctctag gaagcatgga cggtatagaa gatggatcag gagattttta
      421 atttttttaa caaacaaatc aaaaaagatt ttggtaaaac ggcgagtaaa gagacttttg
      481 ctaagtttgc tagttactgc gccgaaggaa tcgaaaaaaa tggagttaag ccaattttta
      541 attggataaa cctatacgct tttggaaccg gtatgacaac agcagaagca gaccgattaa
      601 gaatagagcg atataaacag gagaatgtgt tatgacaaaa cagcatagag aaacgcttat
      661 ctggtacaga gcaagccatc aagagcgtga gagattgctt gatttcgggc ttgttgataa
      721 atcacagtac atgacactat tgcggcaatt gcgcaagaaa tatgcgattt aggagaaaat
      781 atgacaccag aacaagcaga aaaagcaaaa atcagagcta aacaagaact tgaaacgttt
      841 agcatatacc ttgatcaggc aattgatgag ctcggtggag ttctaacttc acgagaagtc
      901 tttttagcag cgggaataac atatcttggc gcaggtcaga cagatataca tgctgcagtc
      961 gagggattat gtgagcaaat ccaatgattt taaatttagt gaagactggg agagcaacta
     1021 aaacacagga agaggaaatg aaatgaacat caaagaaaaa attgtagtgc taagaaacac
     1081 tgaagacgga agttttttaa agagtttcaa aaacaaaaaa gatgtacttg cttataatgt
     1141 ggaacttaca gatagcattc aactggcatc atttttacca gaagaagctt acaacataca
     1201 aaaagaaaaa attgataatt tggcagaaac gcttgggtgt gatgttgtag ttatcgaagc
     1261 atcatatgac ctaaaattta ttgatggcga ggatgttcca gagttaacaa aagagcaaaa
     1321 agttaaaagt atggtaaacg gaatgtttga gcaggttttt ggaggtgaat agagatggca
     1381 aatcaattat caacatacac acacaaacaa ttttttaacg caccaacaat tcaaaaagct
     1441 tttgatgacg tttggaaagg cgccgggaca cagttcgcag taagcatctt gtcagtacta
     1501 caaggtagtc aaagtttgaa atcggcatct aatgaatcta tttatgcagc agctatgaaa
     1561 gcagccgtgt taaatctgcc tattgagcca agcttaggaa gggcctattt agttccgtac
     1621 aaaggtcaag cacaattcca attaggctat aaagggttga ttgagttagc tcagcgcagc
     1681 ggacaataca aaaatattaa tgcaggtatc gtctataaat cacaattaat ttcttacaac
     1741 cctttgttcg aggaattaat ccttgacttt agcaagccac aagatgaaat tgtagggtat
     1801 tttgccgctt ttaaactttt aaatggattc gagaaagttt ctttttggac agtagaaaaa
     1861 gtaactgcac atggaaagaa attttcaaaa tcgtttgcta gcggtccttg gaaaacagac
     1921 tttgatgcaa tggctcaaaa aactatttta aaagatattt tgagtaaata tggtccgtta
     1981 tcagttgaga tgcagaaagc tatcgaggaa gataatcaag attcaacgat ttctacccca
     2041 aaagacatta ccccacaaga agcaaatagc cttgacgacc taattggtca ccagaacgaa
     2101 aataaggatg ctcctaacaa tttaaaagac gtaactgaag atttacatga cgaagaagaa
     2161 aaaacgctcg cagacgaaaa taagacggct ttagaagata cgtcttatcc ggctgatgaa
     2221 atccctgatt tcgaccaaga gacaggcgaa attaaagcta gcgaaggcaa cctctttgat
     2281 aaccttgggg acttaatgcc atgacgaagt tagacttgct cggaaaggac tattatagcc
     2341 gtgaatcagc gattaaatac tggtccatta gtcagtacaa gcgttttaga gagtgcgagg
     2401 caagggcgct tgctgaatta cgagggggtt ggacagatac cagagataac actgcgctgc
     2461 tcgtcgggaa ctatgtccac tcttactttg agagtaaagc agctcatgaa gaattcaaag
     2521 cccaaaacga ctctgaaatg atttcgacca gaggaacaac taaaggtcag ctcaaaaaag
     2581 actatttagt cgcagaacag atgattgaag cacttaaaaa tgacagtaac ttcatggcca
     2641 tataccaagg agaaaaagaa gcagcaatca caggatttct tggcgaggtt gaattcaagg
     2701 gtaaaatcga ctgcttgaat gttgaacgtg gctattttgt agacatcaaa acaacaaaag
     2761 ggccgattga cgacacaatc tggaatagag aagagcgtgt cagatggttt gaagcttatg
     2821 gatatatttt gcagatggct gcttacaaaa ccatgctaga agctaaatac aataagccgt
     2881 ttaagccgat tatttatgca gtgactaaag aaacgccgcc agacacgaga gccatcagaa
     2941 tccaaaatgt agatgctatg caaaatgagt tagacgattt agcccaaaac atcaagcatt
     3001 tagatgatgt taaaaaaggc ctagaaaaac ctaagccttg cggtcattgc gagtattgta
     3061 gagccaatca attaacacaa agagtaatga ttttttagga gatagaaatg actaaatact
     3121 atgtatcagc taagattgca aatttagaca ttggtgcaga aactgaagca gaaaatcaac
     3181 acatggctcc gatagcgttt aaagaaacgt acgaccattt acttagattt ggaagccata
     3241 agctgataat attaggcgtc gaagaactca attaacaacc tattgcaaag tgaagctcga
     3301 cctttgcagt atcaatattt tccgagtgag aaaggaaagt tggaatatcg tcaagttaac
     3361 aggatcgatg atatgaagaa ttgctacact cgtccttgcc aatgctcaca cacaatttta
     3421 gggcgagtgt ggattttaaa aaggtgaaaa atatggaaca aatcaaaatt acaggaacag
     3481 gaacagcact aattttagat agagtaaacc gaatctttgc catctctggc agtttgacta
     3541 tgcaatggga ttttattagt ggttttaaaa agattgacga cgaaccgtca cttgatgaag
     3601 atggagagtt attcgaaaca gcctacgacc tcattcttgc agctaaaccc aaaactaaaa
     3661 tcaatctaac atcttcatac tttgctaaag agcacaagaa agacatagac gaaatcataa
     3721 aagtattctc atttatcgaa gataacaaga gaaatatctt tgaaaccctt ggcattcgcg
     3781 gggtgcttga atgagcaatc tagttttatc gttagacatc tcaacatctg gaacagggtg
     3841 ggccttattt aaaggctcag atcttatcca gagtggtgtc ttaaaacata agagtaaatc
     3901 ctactttgag cgcggccgct atatggctag tcaattaagg ctaatccagt cacgagcatt
     3961 aaaaaaatac gattgctatt ttagtacaat cgcagtcgaa aaaaactcgg ttatgggacc
     4021 taaccagcaa tccatgctta aaatcggtat tgttacagga attatcttag gaagattaat
     4081 agctgataac gtcgtgttta tcaatgtatc aacgtggcgt aagtattgga agtttagcta
     4141 caaagaccga agcaagaagt ccatgaaatt acagtcaaaa ataaaagtgg agcaagaatt
     4201 taataaatcg gtcaaagacg acgaagcaga cgctattttg attggctcat actatgtcaa
     4261 tcaaggctat cttgatgaat tggagacaca tgactactac taaaaacgaa gcgatgcggt
     4321 ttattagaca gctagagtta gctagcggca agcgcgtaaa cgagtactac atgaaggggt
     4381 aaacggatga ctgaagaaca gatgattgat tgcttgcttt atgagttagt aaaaaaagac
     4441 aaagcaatca aaaagaaaag catcattatc gctgcactaa cagttatgct gattgtcgta
     4501 tcagggcttt gcgtatcgct taaaagctat tatgaaccgc aaatatacgg actacgtaca
     4561 cagctaagca ggacacaaaa gcagcttaaa cgtgcgagtg agcaaaatca gagacagaca
     4621 aagcggattg ctgatttaac gaa
//
LOCUS       DAXWIQ010000074         4616 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000074 DAXWIQ010000000
VERSION     DAXWIQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.074"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(293..656)
                     /locus_tag="ACGEUI_001674"
                     /pseudo
     CDS             complement(293..656)
                     /locus_tag="ACGEUI_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1077..1610)
                     /locus_tag="ACGEUI_001675"
     CDS             complement(1077..1610)
                     /locus_tag="ACGEUI_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFF8166490.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGVSYYCNLASPYMMDTEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEIHKKEMQYSADMDFILKENVKILVDWINHEKGPFS
                     KAYITIWYKRYLELKNR"
     gene            complement(1690..2466)
                     /gene="recX"
                     /locus_tag="ACGEUI_001676"
     CDS             complement(1690..2466)
                     /gene="recX"
                     /locus_tag="ACGEUI_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFF8166491.1"
                     /translation="MKITKIEKKKRLYLIELDNDESLYVTEDTIVRFMLSKDKVLDND
                     QLEDMKHFAQLSYGKNLALYFLSFQQRSNKQVADYLRKHEIEEHIIADIITQLQEEQW
                     IDDTKLADTYIRQNQLNGDKGPQVLKQKLLQKGIASHDIDPILSQTDFSQLAQKVSQK
                     LFDKYQEKLPPKALKDKITQALLTKGFSYDLAKHSLNHLNFDQDNQEIEDLLDKELDK
                     QYRKLSRKYDGYTLKQKLYQALYRKGYNSDDINCKLRNYL"
     gene            2581..3936
                     /gene="rlmD"
                     /locus_tag="ACGEUI_001677"
     CDS             2581..3936
                     /gene="rlmD"
                     /locus_tag="ACGEUI_001677"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFF8166492.1"
                     /translation="MVVKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIFC
                     QITAVKRNFAEAKLLTVNKASKNRVKPACSVYETCGGCQIMHLAYPKQLDFKDDVIRQ
                     ALKKFKPTGYEQFEIRPTLGMKKPDHYRAKLQFQLRSFGGTVKAGLFSQGSHRLVPID
                     NCLVQDQLTQDIINKITQLVDKYKLPIYNERKIAGIRTIMVRKAQASDQVQIIVVSSK
                     EVRLANFIGELTKAFPQVKTVALNSNRSKSSEIYGDETEILWGQEAIHEEVLDYGFAL
                     SPRAFYQLNPQQTEVLYGEVVKALDVGSKDHIIDAYCGVGSIGFAFAGKVKSVRGMDI
                     IPEAIEDAQKNAKAMGFDNAYYEAGKAEDIISKWYKQGYRADAVIVDPPRTGLDDKLL
                     KTILHYQPKQMVYVSCNTSTLARDLVQLTKVYDVHYIQSVDMFPHTARTEAVVKLQKR
                     V"
BASE COUNT         1404 a          724 c          965 g         1523 t
ORIGIN      
        1 ctatttagta actttggtta aagtaatcga actaacttgg ttgattgtat tttaatgata
       61 aaaagcttaa atttgttgta taacaaattt aagcttttta ttgtttattt ttaattgcag
      121 attggggttt aaaagacact ttacaatgct ctcaagtcat gtgttgttgt aggttgtatg
      181 catatattac aaatcccatt ataaaagaaa ttgtttaatt aatggatagt aatggatggt
      241 atggagatct taatgtggtt ttaatggtaa ttttagcagt tatagtaaga caaaaggtga
      301 cttggaaaag tttatgcaga cgattcttaa ctcccacaat atcttctgtc agattttatt
      361 agacttcttc tcagtgtcat ttttgtctgt tttacgaacg tgcaggctat tcaattgctt
      421 cttagtttct aaaggattaa accgagtata agcgtagcca ttatctgcga aaaaacctga
      481 agacgacgag aatagacatt aatcgtttta aggataatct cagggttatg gacggttttc
      541 aagtttccaa gaagatgatt gaggccgatg gtatcgtttt gtcttgaatg attccttatt
      601 ttagtggttt cattttcaat acacgacgtc aagcgtccta catactttga taaaattcta
      661 cctaaaagag gtgtcttgtc agttttggtt acaacatcaa gtgccaaaag actttgcgac
      721 ttaacaagac acttctactt taacgcaaag aaaaagtagt gagtactctc tttcgtcgga
      781 gatttcctcg ctactaaact tagtattttt tgatactttc cttaggtagt gcttaaggca
      841 gcgactttct ttggaattcc atgccttatt tttgagatcc taggctcaaa aatgccaagt
      901 ggcgtcaaca ttaacattga cgccactttt accacaacga aaaacatctg ttttggctca
      961 ctccacacgc agaaatagga aattggatgt caaatcagca gagctgttca tgctttttga
     1021 gcctaaaact gagtttgtct gcgttctgag cactccttgg gagtgctgga caactcttaa
     1081 cgattcttca gttcaagata acgtttgtac caaatagtga tataggcctt ggaaaatggt
     1141 cctttttcat gattaatcca atcaaccaat attttgacat tttcttttag gataaagtcc
     1201 atatcggctg aatactgcat ctctttttta tgaatctcgt attcatcaac atcaagtaat
     1261 ctcttttcac catccgcaaa gactttgaca tctaaatcgt aatcaatgta tttaagggcc
     1321 tcagtgtcca tcatgtatgg actagctaag ttacagtaat aagagacgcc attatctcga
     1381 atcatggcta taatgttaaa ccagtatttt ttatgaaagt aaacgattgc aggttcgcgg
     1441 gtcacccatc gtctaccgtc actttctgtg acaagagtgt gatcattaac tccaatgagg
     1501 gcattttcag ttgtttttag taccatagta tcgcgccaag tacggtgcaa actaccatca
     1561 tgcttataac tttgaattgt aataaagtcg ccttctttag gtaatttcat gccttaccaa
     1621 ctttctacaa ttaaacttat cctctatagt ctatcataaa tgtgtgaaaa aatcacttct
     1681 tttgtcagtc tataaataat ttcttaactt gcaattaatg tcgtcgctgt tgtagccttt
     1741 tcggtagaga gcctgataga gcttttgctt taaggtataa ccatcatatt tgcgactgag
     1801 tttacgatat tgtttgtcta attctttgtc aagaagatct tctatttctt gattatcttg
     1861 gtcaaaatta aggtgattga ggctatgttt agctagatcg tatgaaaagc ctttggtcag
     1921 taatgcttgg gtgattttat ctttcaaggc ttttggtggc aatttttctt gatatttgtc
     1981 aaagagtttt tggcttactt tttgagcgag ttggctaaag tcagtttgag ataagatagg
     2041 atcaatgtca tgacttgcaa tgcctttttg taataatttt tgttttaaga cttggggacc
     2101 tttatcacca tttaactgat tttggcgaat gtaggtatca gccaatttgg tgtcgtctat
     2161 ccattgttct tcttggagtt gagtgatgat gtcagcaata atgtgttctt caatctcatg
     2221 cttgcgcagg taatcagcaa cttgcttgtt gctgcgttgt tgaaaggaaa gaaaataaag
     2281 ggctaaattt ttgccgtagg acagttgggc aaaatgtttc atgtcttcaa gctgatcatt
     2341 gtcaaggact ttatctttac tcaacataaa ccgaacaata gtatcttctg ttacataaag
     2401 ggattcgtca ttatccaatt cgataaggta gaggcgtttt ttcttttcaa ttttagtgat
     2461 tttcatactt tgattatatc aaaaatggta aaatagaaga cgaaaatact gctaaaaatg
     2521 tgttgcttgt gagtgctttt ggtggtgaga ttaggatact agaggaagta aggggaagag
     2581 atggtcgtaa aggttaaaca gaaaattcct ttaaaaatta agcgaatggg aattaacggt
     2641 gaggggattg gtttttatca gaaaactttg gtatttgtcc ctggtgcttt aaagggagaa
     2701 gatatttttt gccaaatcac ggcggttaaa cggaattttg cagaggccaa actgttaacg
     2761 gtaaacaagg cttctaagaa tcgtgtcaaa ccagcttgtt ctgtttatga aacttgtggt
     2821 ggttgccaaa tcatgcatct agcctatcct aaacagctag atttcaaaga tgatgtgatt
     2881 aggcaggccc ttaaaaagtt caaaccaact ggctatgaac aatttgagat tagaccgact
     2941 ttaggaatga aaaagccaga tcactatcgg gccaaattgc agtttcagct gcggtcattt
     3001 ggcgggacgg tcaaggctgg tttattttcc cagggaagtc atcgcttggt tcctattgac
     3061 aattgcttgg tacaggatca actaacgcaa gatattatca ataagattac gcagttagtg
     3121 gacaagtaca agttgcccat ttacaatgag cgcaagattg ctgggattcg gacgataatg
     3181 gttcgtaagg cacaggctag tgatcaggta cagattattg ttgtgagtag caaagaagtt
     3241 aggttagcga atttcattgg cgaattgact aaggcatttc ctcaggttaa aacggttgcc
     3301 ttgaatagta atcgttctaa atcgagtgag atttatgggg acgaaacgga aattctatgg
     3361 ggacaagagg cgattcatga agaagtttta gactatggat ttgcgttatc accgagagct
     3421 ttttaccaac ttaatccaca gcagacagaa gttttgtatg gtgaggtggt gaaggccttg
     3481 gatgtaggga gcaaagatca tattattgat gcctattgtg gggttggctc gattggcttt
     3541 gcttttgcag ggaaggtcaa gtctgtgcgg gggatggata ttatcccaga agcgattgaa
     3601 gatgcacaaa aaaatgccaa agccatgggc tttgacaacg cttactatga ggcagggaag
     3661 gcagaggata ttatttctaa atggtataaa caaggttacc gcgcggatgc cgtcattgtg
     3721 gacccgccaa gaactggttt agatgataaa ttattaaaaa cgattcttca ttaccagcct
     3781 aaacagatgg tttatgtgtc ttgtaatacc tccactttgg cccgcgactt ggtgcagcta
     3841 acaaaggttt acgatgttca ctatatccaa agtgtagaca tgttccccca tacagctagg
     3901 accgaggctg tcgtgaagtt acaaaaacgt gtatgattga gtggcaataa caactcaagt
     3961 tttataagct caggtaatgg gtttgagcaa tagcttgaca tagtagggat gtcaaaaacg
     4021 gtttactaag catagtcata ttattgataa ctctatgttt attttagaaa cattcatggt
     4081 taaaaaagtt tgttagtttg tcactaagta gggtctattt gaagagcaag tacacacaga
     4141 caatattagg ataattggtt gttgaaaaaa agctgttttt tgctataaat tagaaaactg
     4201 agtggtcagg aggttacatt accagaagtt attttagtat gctaatatga acgatgatat
     4261 agaggattta tcctgattta atcttttaag ctgtaagaaa cctttgcaaa aactgtaggc
     4321 tgtgtgagtc tttatgtgtt agatattaaa gataaatgca acactttttt ataatgagat
     4381 caacaactga taaatctgag ttgataacca gtaaaatgat cggaatgtag aaaagttact
     4441 taatcaagct tttcgtaatt taactgttag aatgacagaa cttatgataa aataaagaat
     4501 atgattaaaa tgaaaaagct cagtttatct attacggagt tcgaaatgaa aagaaaatcg
     4561 agatgtgata acatattttg aacgagaagg agaaggataa atgttagaag attttc
//
LOCUS       DAXWIQ010000075         4503 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000075 DAXWIQ010000000
VERSION     DAXWIQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.075"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(139..234)
                     /locus_tag="ACGEUI_001678"
     CDS             complement(139..234)
                     /locus_tag="ACGEUI_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFF8166493.1"
                     /translation="MCEAIFTTIIAPLLVGIILLLIQKWLDDSAD"
     gene            870..1061
                     /locus_tag="ACGEUI_001679"
     CDS             870..1061
                     /locus_tag="ACGEUI_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166494.1"
                     /translation="MPDNTIAIEKIKKYLLDNNLKQVDLAVTYGKEPQDVANILAGRK
                     KDPASNRFVLKVISDLKIR"
     gene            1073..1789
                     /locus_tag="ACGEUI_001680"
     CDS             1073..1789
                     /locus_tag="ACGEUI_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFF8166495.1"
                     /translation="MNQIINITVNDNHEPVVSGRDLHKVLEIKTAYKDWFPRMAEYGF
                     EEGQDFSSFLSKSTGGRPSQDHVLKLDMAKEIAMLQRNEKSKQVRKYFIQVEKDFNSP
                     EKIMARALLMADKKVHKLEAQIEADRPKVLFADAVSASKSSCLIGELAKILKQNGINI
                     GQNKLFQWLRANGYLISRRGESWNQPTQKSMQLGLFELKKTAINHSDGHTTTNVTPKV
                     TGKGQQYFINKFLNQEYLPV"
     gene            1821..2087
                     /locus_tag="ACGEUI_001681"
     CDS             1821..2087
                     /locus_tag="ACGEUI_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166496.1"
                     /translation="MEEKKFFTTYEMDLMNSVVTLQKSILTQTEHLSDQLTKKLHHLE
                     DYNSPIDDEAIRLAEVTAEFYKLLIKSPSVGATVKEIVSEGHKR"
     gene            complement(2022..2828)
                     /locus_tag="ACGEUI_001682"
     CDS             complement(2022..2828)
                     /locus_tag="ACGEUI_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HFF8166497.1"
                     /translation="MLNIDTQFVDTIGKILSDYVSHSEITRMGEVLGYPQNDQNSGFN
                     KHKRVHNLMSDILNRTQNTDNIKLVIEYVCNPLRYINEVSIFEQLRTAINIPLSLKGL
                     IVSDSGQIVTTTTSKTLSEAKKRFETLDSRLKELKVHPHVLKFCTQELLQENYFHAVF
                     EASKGVFHRIRLLTGSAMDSASLIDQCFKPGEPIVIINGNKLQTLDEQSEYKGLKNLL
                     LAIAHLYRNSKAHKLKYYNPDNLNDALTALTLMSLAHNLLDSCSNTRRLD"
     gene            complement(2970..3209)
                     /locus_tag="ACGEUI_001683"
     CDS             complement(2970..3209)
                     /locus_tag="ACGEUI_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166498.1"
                     /translation="MDTKEFMKVVSKHINQNFNVDNQLVEFVVAELNQMNAPITQKQA
                     QHIVNILEYVSKSTSKSTIAAMTNALLELGVLKGD"
     gene            3376..3561
                     /locus_tag="ACGEUI_001684"
     CDS             3376..3561
                     /locus_tag="ACGEUI_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8166499.1"
                     /translation="MWGKIEALLIEKKMTKYELSQKAGLNQNCLIDLKKGRKKSLKFD
                     DVVKIADVLGVSLDEFR"
     gene            3639..3950
                     /locus_tag="ACGEUI_001685"
     CDS             3639..3950
                     /locus_tag="ACGEUI_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFF8166500.1"
                     /translation="MPKAEITYRAVDVNETASHGDYKHLIQQWEGLTVATAKQWATEM
                     RDHPDFKQFVLNPTHRIVFIDYEGFKLFVQWKSRNRYKTKKETLSEMLENIKFEKRVG
                     A"
     gene            3952..4137
                     /locus_tag="ACGEUI_001686"
     CDS             3952..4137
                     /locus_tag="ACGEUI_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166501.1"
                     /translation="MNKLELFLLVTTIILAIITRVQHEVIKKHNSPENKRRIFREVAL
                     ENSKGWREKRSGGKVVS"
     gene            4237..4476
                     /locus_tag="ACGEUI_001687"
     CDS             4237..4476
                     /locus_tag="ACGEUI_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8166502.1"
                     /translation="MVILRNKEDWRVYPEELARRSRDSVRVVRTCLEELERVGYVRTY
                     RKSLGRGKGIQYYRFCSDKKISEQAFKKMISDFTT"
BASE COUNT         1576 a          748 c          877 g         1302 t
ORIGIN      
        1 agttctttga caattgaata tcgtatgtga tatgatatag gggaagtgaa gatagtttcg
       61 tcgcgcatag gcgaacataa aaaccccttg caaaatccta caaatgcaag gggttttttc
      121 tattttgcaa tagaagcact aatcagcact gtcatcaagc catttctgga ttaataacag
      181 gattattcca accaatagcg gtgcgataat agttgtgaag atagcttcgc acatagacgt
      241 cacctcctta gctaaggcag gtatagtagt gccagtcaat attatatcat gtacgttatc
      301 gattggatag cgtacttttt tgtttaaaag tataaaacac cacctagctg ctatctagat
      361 ggtgctacgg aaattattct gctcgaaatg aaaatgctag agtgtccgat actctagcac
      421 ccaccaattt tgattagctc tctgcccatc ggacagacaa aacaaatctt cgctggattg
      481 ggaaccagca ctgcatggtt tataggtagc aacaacgact ctatacccct aagtcgaata
      541 acggcaatat ttttacgtgt catgccacca ctccatctat ctctaattta aagtgataga
      601 cattttcgac ttagattcat tcctagtttt gcgcgtctag tccgcttcgg ctccgagtag
      661 agcaatgtgt ctatttacct atgagagcac agtacctttc aaaaattctg ctaacaataa
      721 catcagctcc tttctgtaat aaaggtactt tcattataag attgtaagtc aaaaaaatca
      781 agatggttac aaaaattatt gagcagaata acgatttccg tatattttat taatattttc
      841 tacttatttt tgaaaaagga ggtatacaaa tgcccgataa cacaatcgcc atcgaaaaaa
      901 ttaaaaagta tttgcttgat aacaacctta aacaagttga tctagcagtt acttatggca
      961 aagaaccgca agatgtggcg aacattcttg ctggacgaaa aaaagaccca gcatcaaatc
     1021 gctttgtttt aaaagttatt tcagatttaa aaatcagata gaaaggcatc aaatgaatca
     1081 aataatcaat attacagtaa acgataacca tgaaccagtt gtcagtggtc gggacttgca
     1141 caaagtgctt gaaattaaaa cagcttataa agattggttt ccaagaatgg ccgaatatgg
     1201 ctttgaagaa ggtcaggact ttagctcatt tttgagcaaa agtacaggag gtcgccctag
     1261 tcaagaccat gtacttaaac tagacatggc taaagaaatt gcgatgttgc aacggaatga
     1321 aaaatcaaag caagtccgca aatactttat tcaggtcgaa aaggacttta atagtcccga
     1381 gaaaatcatg gcaagagccc tgctgatggc agacaagaaa gtacataaat tagaggctca
     1441 gattgaggct gaccgtccaa aggtgctatt tgctgatgct gtaagtgcta gcaaatcatc
     1501 ttgtctgatt ggtgagctag ctaaaatcct gaaacagaat gggatcaata ttggtcagaa
     1561 taagctattt cagtggttac gagccaacgg ctacctaatc agtcgccgcg gtgagtcttg
     1621 gaatcagcca acgcaaaaaa gtatgcaact tggattgttt gaactcaaaa aaacagccat
     1681 caatcactct gatggtcaca ctacaacaaa tgtaacccca aaagttactg gtaaggggca
     1741 acaatacttt attaataaat tccttaatca ggaatatcta ccagtttaga aacgtaacaa
     1801 aactaacgaa gggagaaaat atggaagaga aaaaattttt tactacatat gaaatggatc
     1861 tgatgaattc agttgtaact cttcaaaaat caatcttaac gcaaacggaa catttgtcag
     1921 atcaactaac aaaaaagctt catcacctag aagactacaa tagtccgatt gatgatgaag
     1981 caattagatt ggctgaggta acagcagaat tttacaagtt gttaatcaag tctcctagtg
     2041 ttggagcaac tgtcaaggag attgtgagcg agggacataa gcgttaaggc cgtaagagcg
     2101 tcattgagat tatctggatt gtagtatttg agtttgtgag ctttagagtt gcgataaagg
     2161 tgagcgattg caagtagcaa attttttaat cccttatact cactttgttc gtctagagtc
     2221 tgcaatttat taccgttgat aatcacaatg ggttctccgg gtttgaaaca ttgatctatc
     2281 agactagctg agtccattgc tgagcccgtt agtagacgaa tacgatgaaa aacaccttta
     2341 cttgcttcaa atacggcatg aaaatagttt tcttgtaaga gctcttgagt gcagaatttt
     2401 aaaacgtgag ggtgtacttt taattctttt aacctactat caagtgtttc gaagcgtttt
     2461 ttggcctctg ataaagtttt tgaagtagta gtagtaacaa tttgtccgct atctgataca
     2521 atcaaacctt ttagcgagag cggaatgtta atggcggttc ttaactgttc aaaaattgaa
     2581 acttcattaa tgtatcttaa aggattacag acgtattcaa tcacaagttt tatattatct
     2641 gtattctgtg tcctatttaa tatatcggac atgagattgt gcacccgttt gtgcttgtta
     2701 aatccagaat tttggtcgtt ttggggataa cctaaaactt cgcccattcg ggttatttca
     2761 gaatgcgaaa catagtcaga tagtatttta ccgattgtat caacgaattg ggtgtcaata
     2821 tttaacataa caaacctcgt ttttatttaa attataccac agaaaggaga accacatgag
     2881 accaaaacga tatccgtata gcggagagaa aaagagagcc tatcataaga tagaccctga
     2941 actgataaaa accggtaaag ttgattgcat caatctccct taagtacgcc aagctctaac
     3001 aatgcatttg tcatagctgc gatagtagat ttagaggttg acttagagac atactccaaa
     3061 atattaacga tatgctgagc ttgcttttgt gtaataggtg cattcatttg attaagttcc
     3121 gcaacgacaa attcaactaa ttggttgtca acattgaaat tttggttgat atgttttgag
     3181 acaactttca taaattcttt tgtatccata attaatctcc tttcttgctt atttgactaa
     3241 acaagattag aagaatagag gtctctcgag agatcttgtt gaatcatacg aatattatat
     3301 caagactata ttgaaaaatc aatatattgt atataaaaag ttatcaacaa ctacatgttg
     3361 tgcttaggag gaattatgtg gggtaaaatc gaagctctat taattgagaa aaaaatgacc
     3421 aaatatgaac tgtcacaaaa ggctggattg aaccaaaatt gcttgattga tctaaagaaa
     3481 ggaagaaaaa aatcattgaa atttgatgat gtcgttaaaa tcgccgatgt cttaggtgtc
     3541 agcttggacg aattcagata acaaaaaaag tccgacggca atcggactca aaactagatt
     3601 aatttactta attataccac aagagagagg atattaatat gccaaaagca gaaattactt
     3661 atagagcagt tgatgttaat gaaacagctt ctcatggtga ttataaacac ttgattcaac
     3721 aatgggaggg gttgacagtt gctactgcaa agcagtgggc gacagaaatg cgtgatcatc
     3781 cagactttaa acagtttgta ttaaatccaa cgcatagaat cgtattcatt gattacgaag
     3841 gattcaaact gtttgtacag tggaaatctc gcaatcgtta taaaacaaaa aaagagacac
     3901 tgtcagaaat gcttgaaaat attaaattcg aaaaaagagt aggagcataa catgaacaaa
     3961 ttagaactat ttttattagt aacaacgatc atcttagcaa tcatcactag agtacaacac
     4021 gaagtcatta aaaaacataa ttcgccagag aataaacgca gaatttttag agaagtggct
     4081 ttagaaaaca gcaaagggtg gagagagaag cgttctggag gaaaggtggt cagctgatgc
     4141 agtacatttt tcaaaaatac gacaagtagc tatacatccg ttagcaacga atttatagat
     4201 gattgtgaat tatccctaaa atcaaaaggc ctgcttatgg taattcttag aaataaagaa
     4261 gattggagag tatatccaga ggaattggct agaaggtcca gagatagcgt gagagttgta
     4321 aggacttgct tagaagaatt agagagagtc ggatatgtga gaacttacag aaaatcttta
     4381 gggcgaggga aaggcataca atattataga ttttgctcag ataaaaagat atccgaacaa
     4441 gcatttaaaa aaatgattag tgattttaca acttaacgtt ttacaaagtt gtattttaca
     4501 aag
//
LOCUS       DAXWIQ010000076         4439 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000076 DAXWIQ010000000
VERSION     DAXWIQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.076"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..290
                     /locus_tag="ACGEUI_001688"
     CDS             <1..290
                     /locus_tag="ACGEUI_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFF8166503.1"
                     /translation="NKIIIDISSLSLKNNRLDNLEKAIEMSQKIMAKIQDWNRMPILK
                     LIEWKYFLIKQKDIIKAEQSFMKACLFAQMTADQYLENKLIQEWEKDVKSY"
     gene            374..469
                     /locus_tag="ACGEUI_001689"
     CDS             374..469
                     /locus_tag="ACGEUI_001689"
                     /inference="COORDINATES: protein motif:HMM:NF038244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system DWW-type pheromone"
                     /protein_id="HFF8166504.1"
                     /translation="MLKKYKYYFIFAALLSFKVVQELSAVDWWRL"
     gene            516..1514
                     /gene="ruvB"
                     /locus_tag="ACGEUI_001690"
     CDS             516..1514
                     /gene="ruvB"
                     /locus_tag="ACGEUI_001690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023079556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFF8166505.1"
                     /translation="MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEA
                     AKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAVEKAGDLVAILNELE
                     PGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPR
                     IANRLLKRVRDYAQIIGDGIITAQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGG
                     PVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQKAYRHLGYPYQNT"
     gene            1652..2089
                     /locus_tag="ACGEUI_001691"
     CDS             1652..2089
                     /locus_tag="ACGEUI_001691"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFF8166506.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMKSIVSSDVMMIESRATSDWEHG
                     NPIHSGTQSILKTYQINYDITKCSKQITITDFNTFDYIIGMDSDNVKNLKEMSQHQWD
                     SKIYLFREGGVPDPWYTNDFEETYQLVRKGCQDWLSRLMSKEY"
     gene            2112..2513
                     /locus_tag="ACGEUI_001692"
     CDS             2112..2513
                     /locus_tag="ACGEUI_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFF8166507.1"
                     /translation="MIDMQDIVKKWSITRAKLEIVSVIVILVCAISVFSVRISNKTSL
                     TYDKGRMHYTGYVINHKMNGEGKLVYPNGDIYEGTFKDGLFEGKGTFTAKTGWLYNGE
                     FHKGQANGKGVLKAKNNKVYKGIFKQGIFQK"
     gene            2510..4285
                     /locus_tag="ACGEUI_001693"
     CDS             2510..4285
                     /locus_tag="ACGEUI_001693"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFF8166508.1"
                     /translation="MRIKWFSFVRVTGLLLVLLYHFFKNVFPGGFIGVDIFFTFSGYL
                     ITALLIDEYTKKESIDIIGFLKRRFYRIVPPLVLMILLTIPFTFLIKKDFIANIGSQI
                     TAVLGFTTNIYEILTGSSYESQFIPHLFVHTWSLAIEVHFYLFWGVFVWLLARRKETQ
                     KQLRGLLFLISLGIFAISFLSMFIRSFMTSNFSLIYFSSLSHSFPFFLGAMFATITGI
                     NETTVRFQKNVRLWPRQYVLAAMIGAFTLLLVLTVTLDFNHITTYLFGFALASLFASI
                     MIYAARVLHEQTPDVQEPKAITYIADISYGIYLFHWPFYIIFSQLMSHILAVILTVFF
                     SILFATVSYYIVEPLVQGRKPNLLGLEIDCSPYYKWIVGGSLALALLTLGTCMIAPKV
                     GKFEKQLLVSSLQQAQSNMERTHTLAAGDANALSDVGIIGDSVALRSSAAFSQIMPQA
                     QLDAAVSRNFKEAFDLFNNQIKSKSLSKTVVLAVGVNSLDNYSQAVQSFIEALPKGHR
                     LVLVSPYNAKNASQVAEARDYGLKLSKKYKYVTIADWYKVAVEHPDIWYGSDGVHYSE
                     DSQGAELYVSTIQTAVEKSAKKPAK"
BASE COUNT         1409 a          672 c          892 g         1466 t
ORIGIN      
        1 ttaataagat tatcattgat atttcatcac taagcttaaa aaataaccga cttgataatt
       61 tagaaaaagc gattgaaatg agtcaaaaaa ttatggcgaa aattcaagat tggaatagaa
      121 tgcctatttt aaaactaata gaatggaaat attttttaat caaacaaaaa gatataataa
      181 aagcagaaca atcatttatg aaggcatgtt tgttcgccca aatgacagct gatcagtatt
      241 tggaaaacaa gttaattcaa gagtgggaaa aagatgttaa aagttattaa caggacaaaa
      301 atgtcatttt taaatattta atagtaagat ataattccta atataaaaaa taattattaa
      361 ggagttttta tccatgttaa aaaagtataa gtactatttt atattcgcag ctctactatc
      421 ttttaaagta gttcaagagc ttagtgctgt tgactggtgg cgattataat aaaagtaggc
      481 cactgctctc ttttacttta tgttataata gtcttatggc tagaatttta gacaataatg
      541 taatgggaaa tgaggagttt tcggatcgga ctcttcgccc tcaatatcta cacgaatata
      601 ttggacaaga taaagttaag gaacaattcg ctatttttat agaagcagct aaaagacgtg
      661 acgaatcttt agaccatgtt ttattatttg gacctccagg actgggaaaa acaacaatgg
      721 catttgtgat tgccaatgaa ttgggagtta atttaaagca aacttcaggt cctgccgttg
      781 agaaagctgg agatttggta gctattttaa atgaattaga accaggggat attttgttta
      841 ttgatgaaat tcaccgcatg ccaatgtctg tcgaggaagt actatatagt gctatggaag
      901 acttctatat tgatattatg attggcgctg gagatactag tcgtagtatt catttggatc
      961 tgcctccatt tacattgatt ggtgctacta cacgtgcagg catgttatct aatcccctca
     1021 gagctcgttt tggtatcact ggtcacatgg aatactatca agaaaaggat ttgacagaga
     1081 ttgtggagcg tacagctact atttttgaaa taaaaataga ccatgaagct gcacggaaat
     1141 tagcttgtcg cagtagagga acgccccgca ttgctaatcg attactaaaa cgagtgcgtg
     1201 actatgctca aattattggt gatggcatca taacggctca aataactgat agagctttga
     1261 ctatgttaga tgttgaccgc gaaggtctcg attacattga tcagaaaatt ttacgaacga
     1321 tgattgagat gtatcaggga gggccagttg gtttggggac attatctgtg aatattgccg
     1381 aggagcgtaa tacagttgaa gagatgtatg aaccctactt aattcaaaaa ggctttctca
     1441 tgcgaacacg aacaggtcgt gtagcgacgc aaaaggctta tcgtcatctt ggctatccct
     1501 atcaaaacac ttaatgattt ttcaaatcta agaaaattaa tctgaacctg ctttttgtgt
     1561 tcggattttt tgataagttg acagaaaatt attagattga tttctccgat atcagttaga
     1621 aatttgatat aataattcaa aggagtattt aatgaaaaaa gtatgttttg tctgtctggg
     1681 taatatttgt cgaagcccca tggctgagtt tgtaatgaag tcaatagtat caagtgatgt
     1741 tatgatgata gaaagtcgtg ctacttctga ttgggagcat ggaaacccta ttcactcagg
     1801 tacacagtcc atattaaaaa cttaccaaat taattatgat ataactaaat gctccaagca
     1861 gataactata actgatttta acacctttga ctatattatt ggtatggata gcgataatgt
     1921 gaaaaatctc aaagagatgt cgcaacatca atgggactct aaaatctacc tgtttagaga
     1981 agggggtgtt cctgatcctt ggtacactaa cgattttgaa gaaacctacc aattagttag
     2041 aaaaggatgc caggattggc tttctcgttt aatgagtaaa gagtattgat attagaagtt
     2101 gagtaaaaga aatgatagat atgcaagata tagtaaaaaa atggagtatt acacgggcga
     2161 agttggaaat tgtctcggtg attgtcatat tggtttgtgc aatttctgtt tttagcgtaa
     2221 gaatttcgaa taagacaagt ttgacctatg ataaagggcg tatgcactat actggttacg
     2281 tgattaacca taaaatgaat ggagaaggga agcttgtcta ccccaacggt gatatctatg
     2341 agggtacttt taaggatggc ctatttgaag gaaaaggaac ttttactgct aagacgggct
     2401 ggttatataa cggggagttt cataaaggac aagcaaatgg caaaggtgtg ttaaaagcaa
     2461 agaataataa agtctataaa gggattttta aacaggggat atttcaaaaa tgagaataaa
     2521 atggttttcg tttgttaggg taacaggact tttactggtt ttactctacc acttttttaa
     2581 aaatgtattt ccaggaggtt ttattggtgt tgacattttc ttcacctttt caggatatct
     2641 aatcacagcc ctcttgattg atgaatacac aaaaaaagag tctatcgata ttatcggttt
     2701 tttaaaaaga cgcttttatc gaattgttcc tcctttggtg ttaatgattt tattgaccat
     2761 tccttttacg tttttaatta agaaggattt cattgctaat ataggaagtc agattacagc
     2821 tgttttagga tttaccacta atatttatga aatattgacg ggaagtagtt atgaaagcca
     2881 atttattcca catctctttg ttcatacgtg gagcttagct attgaagttc atttttactt
     2941 gttttggggt gtatttgttt ggttattagc tagacgcaag gagactcaga aacaactgag
     3001 aggactactg tttttgattt ctttgggaat atttgctatt agtttcttga gcatgtttat
     3061 aagatctttt atgacctcaa atttttccct gatttatttt tcgagtcttt ctcacagttt
     3121 tcccttcttt ttaggggcga tgtttgccac gataacaggt attaacgaga ctactgttcg
     3181 atttcaaaaa aatgtacgtt tatggccgcg ccagtatgtc cttgctgcta tgattggagc
     3241 atttacactt ttgcttgtgc taacggtcac attggatttt aaccatatca cgacctatct
     3301 atttggcttt gctttggcta gcttattcgc atcaattatg atttatgcag cgcgtgttct
     3361 tcatgagcaa acaccagatg tacaagagcc taaagctatc acttatatag ctgatattag
     3421 ctatgggatt tacctcttcc attggccatt ttacattatt tttagccaat taatgtctca
     3481 tattttggca gttattctaa cagttttctt ttcaatcttg tttgccactg tgtcttacta
     3541 tattgtggaa cctttggtcc aagggagaaa accaaacctg cttggtttag aaattgactg
     3601 ttctccttac tataagtgga ttgtaggagg gtctctcgcc ttagcattat taactttagg
     3661 aacttgtatg atagcgccta aagttggtaa gtttgagaaa caattgttgg tcagttccct
     3721 acaacaggct caaagtaata tggagcggac acatacacta gctgcaggag acgctaatgc
     3781 tttgagtgac gtaggtatta ttggtgattc tgtagcatta cgctctagtg cagcattctc
     3841 acaaattatg ccacaagcgc aactggatgc agcggtcagt cggaatttta aagaagcttt
     3901 cgatttattt aataaccaaa ttaaatctaa gagtctatct aagactgttg tcttagcagt
     3961 aggtgttaac tcgcttgaca actattctca agcagttcag tcttttattg aggcattgcc
     4021 aaaaggtcat cgtttagttt tggtttctcc atataatgcc aaaaatgcca gtcaggttgc
     4081 agaagctaga gactatggtc ttaaattgtc caaaaaatac aagtatgtca ctattgcaga
     4141 ctggtataaa gttgctgttg aacatccaga tatttggtat ggcagtgatg gtgttcatta
     4201 tagtgaagat agtcaaggag cagaactata tgtctccaca atccagactg ctgttgagaa
     4261 gtcagctaaa aaaccagcta aataaagtta aaaaatgtct aagagataag tcagatccat
     4321 tgtgctctgg ctttttcttg tgaacaaatg atgatggtaa aatagtagta ctttgaggtt
     4381 tttaaaagaa agcggtttca gaaataaaac ggcttttttt agatatattt ctgaaaaaa
//
LOCUS       DAXWIQ010000077         4281 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000077 DAXWIQ010000000
VERSION     DAXWIQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.077"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..776
                     /locus_tag="ACGEUI_001694"
     rRNA            <1..776
                     /locus_tag="ACGEUI_001694"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            828..900
                     /locus_tag="ACGEUI_001695"
     tRNA            828..900
                     /locus_tag="ACGEUI_001695"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:861..863,aa:Ala,seq:tgc)
     gene            1199..4101
                     /locus_tag="ACGEUI_001696"
     rRNA            1199..4101
                     /locus_tag="ACGEUI_001696"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4187..>4281
                     /gene="rrf"
                     /locus_tag="ACGEUI_001697"
     rRNA            4187..>4281
                     /gene="rrf"
                     /locus_tag="ACGEUI_001697"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1205 a          890 c         1213 g          973 t
ORIGIN      
        1 agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccgtaaac gatgagtgct
       61 aggtgttagg ccctttccgg ggcttagtgc cggagctaac gcattaagca ctccgcctgg
      121 ggagtacgac cgcaaggttg aaactcaaag gaattgacgg gggcccgcac aagcggtgga
      181 gcatgtggtt taattcgaag caacgcgaag aaccttacca ggtcttgaca tcccgatgcc
      241 cgctctagag atagagtttt acttcggtac atcggtgaca ggtggtgcat ggttgtcgtc
      301 agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag cgcaacccct attgttagtt
      361 gccatcatta agttgggcac tctagcgaga ctgccggtaa taaaccggag gaaggtgggg
      421 atgacgtcaa atcatcatgc cccttatgac ctgggctaca cacgtgctac aatggttggt
      481 acaacgagtc gcaagccggt gacggcaagc taatctctta aagccaatct cagttcggat
      541 tgtaggctgc aactcgccta catgaagtcg gaatcgctag taatcgcgga tcagcacgcc
      601 gcggtgaata cgttcccggg ccttgtacac accgcccgtc acaccacgag agtttgtaac
      661 acccgaagtc ggtgaggtaa cctattagga gccagccgcc taaggtggga tagatgattg
      721 gggtgaagtc gtaacaaggt agccgtatcg gaaggtgcgg ctggatcacc tcctttctaa
      781 ggaaatggaa cacgtttatc gtcttattta gttttgagag gtcttgtggg gccttagctc
      841 agctgggaga gcgcctgctt tgcacgcagg aggtcagcgg ttcgatcccg ctaggctcca
      901 tcaggataca atcctactaa acttaataca agtgaagttg aacacgcaac tcacttccta
      961 ggaaaatgga caatcttccc ttgtgtgcaa ggcccacatg gtcagattcc taattttcta
     1021 cagaagtttc gctaaagcga gcgttgctta gtatcctata taatagtcca ttgaaaattg
     1081 aatatctata tcaaattcca cgatctagaa atagattgta gaaaagtaac aagaaaataa
     1141 accgaaaacg ctgtgactat ttaataagtt ttctagttta aaaaaactag gttaataagg
     1201 ttaagttaat aagggcgcac ggtggatgcc ttggcactag aagccgaaga aggacgtgac
     1261 aaacgacgaa atgctttggg gagctgtaag taagcgctga tccagagatg tccgaatggg
     1321 ggaacccact aactaatggt tagtatccat aactgttaag gttatgagaa ggaagacgca
     1381 gtgaactgaa acatctaagt agctgcagga agagaaagca aacgcgattg ccttagtagc
     1441 ggcgagcgaa acggcaggag ggcaaaccga agtgtttaca cttcggggtt gtaggactgc
     1501 gacgtgggac tttaaaagga tagaagaatt acctgggaag gtaagccaaa gagagtaaaa
     1561 gcctcgtatt taaaattctt ttgagcccta gcagtatcct gagtacggcg agacacgtga
     1621 aatctcgttg gaatctggga ggaccatctc ccaaccctaa atactctcta gtgaccgata
     1681 gtgaaccagt accgtgaggg aaaggtgaaa agcaccccgg gaggggagtg aaatagaacc
     1741 tgaaaccgtg tgcctacaac aagttcgagc ccgttaatgg gtgagagcgt gccttttgta
     1801 gaatgaaccg gcgagttacg atatgatgcg aggttaagtt gaagagacgg agccgtaggg
     1861 aaaccgagtc ttaatagggc gatttagtat catgttgtag acccgaaacc atgtgaccta
     1921 cccatgagca ggttgaaggt gaggtaaaac tcactggagg accgaaccag ggcacgttga
     1981 aaagtgcttg gatgacttgt gggtagcgga gaaattccaa acgaacttgg agatagctgg
     2041 ttctctccga aatagcttta gggctagcgt cgatgttaag tctcttggag gtagagcact
     2101 gtttgggtga ggggtccatc tcggattacc aatctcagat aaactccgaa tgccaacgag
     2161 atatcatcgg cagtcagact gcgagtgcta agatccgtag tcgaaaggga aacagcccag
     2221 accaccagct aaggtcccaa aataactgtt aagtggaaaa ggatgtgggg ttgcacagac
     2281 aactaggatg ttagcttaga agcagctatt cattcaaaga gtgcgtaata gctcactagt
     2341 cgagtgaccc tgcgccgaaa atgtaccggg gctaaaacag tttaccgaag ctgtggatga
     2401 cacaaaagtg tcatggtagg agagcgttct atgtgtgaag aaggtgtacc gtgaggagcg
     2461 ctggaacgca tagaagtgag aatgccggta tgagtagcga aagacaggtg agaatcctgt
     2521 ccaccgtaag actaaggttt ccaggggaag gctcgtccgc cctgggttag tcgggaccta
     2581 aggagagacc gaaaggtgta tccgatggcc aacaggttga tattcctgta ctagagtata
     2641 tagtgatgga gggacgcagt aggctaacta aaccggacga ttggaagagt ccggctaagc
     2701 agtgaggtgt aagatgagtc aaatgcttat ctttataaca ttgagctgtg atggggagcg
     2761 aattgtagta gcgaagttag tgatgtcaca ctgccaagaa aagcttctag cgtttaatga
     2821 tactctaccc gtaccgcaaa ccgacacagg tagtcgaggc gagtagcctc aggtgatcga
     2881 gagaactctc gttaaggaac tcggcaaaat gaccccgtaa cttcgggaga aggggtgctg
     2941 acttaggtca gccgcagtga ataggcccaa gcaactgttt atcaaaaaca cagctctctg
     3001 ctaaatcgta agatgatgta tagggggtga cgcctgcccg gtgctggaag gttaagagga
     3061 gggtttagcg caagcgaaga tctgaattga agccccagta aacggcggcc gtaactataa
     3121 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acccgcacga aaggcgtaat
     3181 gatttgggca ctgtctcaac gagagactcg gtgaaatttt agtacctgtg aagatgcagg
     3241 ttacccgcga caggacggaa agaccccatg gagctttact gcagtttgat attgagtatc
     3301 tgtaccacat gtacaggata ggtaggagcc attgacttcg ggacgccagt ttcgaatgag
     3361 gcgttgttgg gatactaccc ttgtgttatg gctactctaa cccagatagg ttatccctat
     3421 cggagacagt gtctgacggg cagtttgact ggggcggtcg cctcctaaag agtaacggag
     3481 gcgcccaaag gttccctcag attggttgga aatcaatcgc agagtgtaaa ggtataaggg
     3541 agcttgactg cgagagctac aactcgagca gggacgaaag tcgggcttag tgatccggtg
     3601 gtaccgaatg gaagggccat cgctcaacgg ataaaagcta ccctggggat aacaggctta
     3661 tctcccccaa gagttcacat cgacggggag gtttggcacc tcgatgtcgg ctcgtcgcat
     3721 cctggggctg tagtcggtcc caagggttgg gctgttcgcc cattaaagcg gcacgcgagc
     3781 tgggttcaga acgtcgtgag acagttcggt ccctatccgt cgcgggcgta ggaaatttga
     3841 gaggatctgc tcctagtacg agaggaccag agtggactta ccgctggtgt accagttgtc
     3901 ttgccaaagg catcgctggg tagctatgta gggaagggat aagcgctgaa agcatctaag
     3961 tgcgaagccc ccctcaagat gagatttccc atgattttat atcagtaaga gccctgagag
     4021 atgatcaggt agataggtta ggagtgtaag tgtagcgata catgtagcgg actaatacta
     4081 atagctcgag gacttatcca aaaagaaata ttgacaacgt tacggattct tgttagacta
     4141 tagatattca attttgagtg ggttatgaaa atagcacatt tacaagttaa gtgacgatag
     4201 cctaggagat acacctgtac ccatgccgaa cacagcagtt aagccctaga acgcctgatg
     4261 tagttggggg ttgccccctg t
//
LOCUS       DAXWIQ010000078         4003 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000078 DAXWIQ010000000
VERSION     DAXWIQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.078"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..868
                     /locus_tag="ACGEUI_001698"
     CDS             <1..868
                     /locus_tag="ACGEUI_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFF8166509.1"
                     /translation="YHVTDDLKPFQVKDLVSGHLERIQEEVEKKYPDAGFNSDKYNGL
                     KESNSLLSDGFEVKSGNLIFDKKLTIPLTTLFDVINPDFLANSDRAAYDNYRTYKEQH
                     PKKLVALTFDDGPDPTTTPQVLDILAKYQAKGTFFMIGSKVVNNENLTKRVSDAGHEI
                     ANHTWDHPNLTNLSVSEIQHQVNMTNQAIEKACGKKPRYLRPPYGATNATVQQSSGLT
                     QMLWTVDTRDWENHSTDGIMTNVKNQLQPGGVVLMHDIHQTTINALPTVMEYLKAEGY
                     ECVTVSELYAHQ"
     gene            complement(979..2064)
                     /locus_tag="ACGEUI_001699"
     CDS             complement(979..2064)
                     /locus_tag="ACGEUI_001699"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8166510.1"
                     /translation="MITKFPPQWQEKLDQVAFTHLTPIQEQAFQPIVDGKNFLGISPT
                     GTGKTLAYVFPSLLALTPKKSQQLLILAPNTELAGQIFEVTKDWAQPLGLTAQLFISG
                     TSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMSVNTIVLDEYDELLGDSQYDFV
                     QKISHYVPRDHQMVYMSATNKVDQTSLAPNTFCIDLSEQTNDAIQHFYLMVDKRERTD
                     LLRKFTNIPHFRALVFFNSLSDLGATEERLQYNGAAAVSLASDINVKFRKTILEKFKS
                     HQLSLLLATDLVARGIDIDNLDYVIHFDVARDKENYTHRAGRTGRMGKSGIVITFVSH
                     PEDLKKLKKFAKVSEISLKNQQLHFIN"
     gene            2162..2788
                     /gene="udk"
                     /locus_tag="ACGEUI_001700"
     CDS             2162..2788
                     /gene="udk"
                     /locus_tag="ACGEUI_001700"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFF8166511.1"
                     /translation="MLKKPIIIGVTGGSGGGKTSVSRAILDSFPNARIAMIQHDSYYK
                     DQSHMSFEERVKTNYDHPLAFDTDFMIQQLKELLAGRPVDIPIYDYKKHTRSNTTFRQ
                     DPQDVIIVEGILVLEDERLRDLMDIKLFVDTDDDIRIIRRIKRDMMERGRSLESIIDQ
                     YTSVVKPMYHQFIEPSKRYADIVIPEGVSNVVAIDVINSKIASILGEV"
     gene            2868..3131
                     /locus_tag="ACGEUI_001701"
     CDS             2868..3131
                     /locus_tag="ACGEUI_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFF8166512.1"
                     /translation="MLLIWLGLLFVWNELVFVIYAFDKRKAIKKKRRISERKLLVITV
                     LFGGFGALLAAKKYHHKTRKWYFVITCYTSILLTLLVTYIFMK"
     gene            complement(3184..3993)
                     /locus_tag="ACGEUI_001702"
     CDS             complement(3184..3993)
                     /locus_tag="ACGEUI_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HFF8166513.1"
                     /translation="MKQITQQKWLRYGLFIALVLNGIELELLGLTANNLSFKEAFALI
                     LTISLLGIYLIPFAAAIFYLSKKFHMNLNVIIVSCLSGLYISGFLASCGNHLVGQFWS
                     YIIPSKDALKLWGDALTAPIVEEPIKASSAILVITLFPRLTLKEKLVVALLSGMGFQL
                     TEDIRYLIQAKSIDSLVPTAIERISTAVTSHWVHTAIFTIGAYLLLKGSNLFSKQQQI
                     FWLLSPLVLHFIWNSPLTSIPGMTVLLGTLILLIFGDLFQKINTLDDDVLF"
BASE COUNT         1318 a          634 c          802 g         1249 t
ORIGIN      
        1 atatcacgtt acagacgact tgaaaccgtt tcaggtaaag gatctagtat caggacattt
       61 agaaagaata caagaagaag ttgaaaaaaa atatccagat gctggtttta atagcgataa
      121 gtataatggc ttaaaagaat ctaattcttt attaagcgat ggctttgagg taaaatcggg
      181 aaaccttatt tttgataaaa agctaacgat acctttgacg acattatttg atgttattaa
      241 tccagatttt ttagcaaata gcgatagagc tgcgtatgat aattatagga cctacaaaga
      301 acagcatccc aaaaaactag ttgcattaac gtttgatgat ggtccagatc cgacgacgac
      361 tcctcaagtt ttagatattt tggcaaaata ccaggctaag ggaactttct ttatgatagg
      421 ttcaaaggtt gtgaataatg aaaaccttac taaacgtgtt agcgacgctg gccatgaaat
      481 tgctaatcat acttgggatc atcctaatct gactaatctt tcagtcagcg agattcaaca
      541 tcaagttaat atgacgaacc aggctattga aaaagcttgt ggcaagaaac ctcgctattt
      601 acgccctcca tacggagcta caaatgcaac tgttcagcaa tcctcaggat taacacaaat
      661 gctttggacg gttgatacaa gagattggga aaatcatagc actgatggca ttatgactaa
      721 tgtcaaaaac caattgcaac cgggaggagt tgttttaatg catgatattc atcaaacaac
      781 tatcaatgct ttgccgacag ttatggaata tctaaaagca gaaggatacg aatgtgtgac
      841 tgtatcagaa ctctatgcgc atcagtaagt aatatcgtta tgtttcttta gaattgatat
      901 actaaacaat ccaattactt ttgaagtaat tggattgttg tttttagtag cccttgatta
      961 agttttgttt tttaacaact agttgatgaa atgaagttgt tgatttttga gggagatttc
     1021 agagacttta gcaaattttt tcaatttttt gaggtcttca gggtggctaa caaaggtaat
     1081 aacgatacca gattttccca tacggccggt tcgtccagct ctgtgagtgt aattttcctt
     1141 atcacgagca acatcaaaat gaatgacata gtcaagatta tcaatatcaa tgccccgagc
     1201 aacaagatcc gtagctaaca ataaggagag ttggtgggat ttgaattttt cgagaatggt
     1261 tttacggaat ttgacattaa tgtcactcgc taaggaaacc gcagcagctc cattatattg
     1321 gagacgttct tccgtagcac ccaaatcaga taaactgttg aaaaagacca aagctctaaa
     1381 atgaggaata ttagtaaatt ttcgtaataa atctgtacgt tcgcgcttgt caaccatgag
     1441 gtaaaaatgc tgaatagcat cgttggtttg ctctgaaaga tcgatgcaga atgtatttgg
     1501 agctaaggaa gtttgatcca ccttattagt agcactcatg tataccattt gatgatctcg
     1561 aggaacatag tggctaattt tttgaacaaa atcatattgg gaatcaccta atagttcgtc
     1621 atattcatct aaaacaatgg tgttgacaga catcatcttg atttttttca atttaatcag
     1681 ctcaaaaatg cgtccagggg ttccaatcag gatttcagga ccttttttga ggcgttcgat
     1741 ttgacgtttt tgactggttc cagaaataaa gagttgtgcc gttaagccta gtggctgtgc
     1801 ccaatccttt gtgacttcaa aaatttggcc agctaattcg gtattagggg ctaaaatgag
     1861 tagttgttgg gactttttag gcgtcagtgc caatagacta ggaaagacat aagctaaggt
     1921 cttgcccgtc ccagtagggc taataccaag gaaattttta ccatcaacaa taggttgaaa
     1981 ggcctgttct tggattggcg ttaaatgggt aaatgcaact tggtcaagtt tttcttgcca
     2041 ttgaggggga aatttagtga tcatgcgtct tccttttact attgtttcta tattcattat
     2101 agcattatag tctcaatttt ggtaaaatag tgagaaagac tttttagatt tggagtttaa
     2161 aatgcttaaa aaaccgatta ttattggtgt aactggtggt tcaggtggcg gtaaaacaag
     2221 tgtgtcacgc gctattttgg acagttttcc aaatgctcgt attgcaatga ttcaacacga
     2281 ttcttactat aaagatcagt ctcacatgag ttttgaagaa agggtcaaga ccaattatga
     2341 ccatcctcta gcttttgata cagattttat gattcagcaa ctaaaagaat tattagctgg
     2401 tagacctgta gatattccta tctatgatta caaaaagcat acccgcagta atacgacctt
     2461 tcgtcaggat cctcaggatg ttattattgt tgaaggtatt cttgtcctag aagatgagcg
     2521 tttgcgagat ttgatggata tcaagctctt tgtagacaca gatgacgata ttcgtattat
     2581 tcgtcgtatt aagcgtgaca tgatggaacg tggcagaagc ttagaaagta ttattgatca
     2641 gtacacaagc gtggtaaagc ctatgtatca tcagtttatt gagccaagta aacgctatgc
     2701 tgatattgtt attccagagg gagttagtaa tgtcgttgct attgatgtta ttaatagtaa
     2761 aattgcgagt attcttggag aagtatagtg gaagtggctg agtagattat ttaaatctgc
     2821 tcttttattg ttgtgctctt catagagaaa gggatcataa aatagaaatg ttattaattt
     2881 ggcttggatt actatttgtt tggaatgaac tcgtgtttgt catatatgcc tttgataaaa
     2941 gaaaagccat aaaaaagaaa cggcgtatta gtgaaagaaa attattagtg ataacagttt
     3001 tgtttggagg ttttggagca ttacttgcag ctaaaaagta ccaccataaa accagaaagt
     3061 ggtactttgt catcacttgt tatactagta ttttgttgac attactagta acttatatct
     3121 ttatgaagtg agtcacttaa aagaccctta atcagtatta tttagtcaag gctcttttat
     3181 agtttaaaaa agcacatcat cgtcaagggt gttgattttt tgaaaaagat caccaaagat
     3241 taaaaggatt aatgttccta gtaaaactgt catgccagga atgcttgtta gaggagagtt
     3301 ccagatgaaa tgtagaacca gaggacttaa taaccagaaa atttgttgct gttttgaaaa
     3361 aaggttagaa ccctttaata ataagtatgc tccaatggta aaaatagctg tatgtaccca
     3421 atgtgaagtc acagcagtgc taatacgttc aatagcagtc ggtacgagac tatctataga
     3481 ttttgcttga atcaaataac ggatatcttc tgttagttga aagcccatgc cagaaagtaa
     3541 agcaacaact aatttttctt taagagtaag tctaggaaat aaagtaatta ctaaaatagc
     3601 cgaagaagcc ttaatgggct cttcgacaat aggggcagtt aaagcatctc cccaaagctt
     3661 taaagcgtct ttagaaggga tgatgtatga ccaaaattgt cctacgagat ggttcccaca
     3721 agaagctaaa aaacctgaaa tataaagtcc gctaagacag ctaacaataa taacgttcag
     3781 gttcatatga aattttttac ttaaataaaa aatagctgct gcaaatggaa ttaagtaaat
     3841 gcctaataga ctaatggtta gtattagagc aaaagcttct ttaaaggaaa ggttgttagc
     3901 agtcagtcct aacaattcaa gttcaatacc atttaaaact aaagcaataa aaagaccgta
     3961 tcgtaaccat ttctgttgag tgatttgttt cacgaaaacc tcc
//
LOCUS       DAXWIQ010000079         3986 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000079 DAXWIQ010000000
VERSION     DAXWIQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.079"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            325..588
                     /locus_tag="ACGEUI_001703"
     CDS             325..588
                     /locus_tag="ACGEUI_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFF8166514.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADAEDAIAAIEETMTKEGLA"
     gene            593..2326
                     /gene="ptsP"
                     /locus_tag="ACGEUI_001704"
     CDS             593..2326
                     /gene="ptsP"
                     /locus_tag="ACGEUI_001704"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFF8166515.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARL
                     DVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMVLADPEMISQVKETIRAKQTNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGD
                     VIAVNGITGEVIIDPSEDQVLAFKEAGAAYAKQKAEWSLLKDAHTETADGKHFELAAN
                     IGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTECSTAEEV
                     LELSKEYVSED"
     gene            2511..3938
                     /locus_tag="ACGEUI_001705"
     CDS             2511..3938
                     /locus_tag="ACGEUI_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019322885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HFF8166516.1"
                     /translation="MAKQYKNLVNGEWKLSENEITIYAPATGEELGSVPAMTQAEVDA
                     VYASAKKALSDWRALSYVERAAYLHKAADILVRDAEKIGAILSKEVAKGHKAAVSEVI
                     RTAEIINYAAEEGLRMEGEVLEGGSFEAASKKKIAIVRREPVGLVLAISPFNYPVNLA
                     GSKIAPALIAGNVVALKPPTQGSISGLLLAEAFAEAGIPAGVFNTITGRGSVIGDYIV
                     EHEAVSFINFTGSTPIGEGIGKLAGMRPIMLELGGKDSAIVLEDADLALAAKNIVAGA
                     FGYSGQRCTAVKRVLVMDKVADQLAAEIKTLVEKLSVGMPEDDADITPLIDTSAADFV
                     EGLIKDATDKGATALTAFNREGNLISPVLFDHVTTDMRLAWEEPFGPVLPIIRVTTVE
                     EAIKISNESEYGLQASIFTTNFPKAFGIAEQLEVGTVHLNNKTQRGTDNFPFLGAKKS
                     GAGVQGVKYSIEAMTTVKSVVFDIQ"
BASE COUNT         1243 a          722 c          875 g         1146 t
ORIGIN      
        1 aaagctaaaa agcaagctat aaacttatct gaaacacaac atattgtgtt gaaataaaaa
       61 acagaaacct aaatattgtg tcaatctgga ttcttatggt tagagctcta gcgttttggt
      121 gatagaagtt aagcagtttt aataaagcat taaagtctga aaaataaatt gtgaaaaacg
      181 tgaaaataaa agaaaataat ttcacatttt ttgtcgttta aatatttaat cttgaaaata
      241 aaaaagcatt gtgctataat cggttgttgt aagggttatc atgtgatggc tcaaaaatac
      301 acaacttaaa aggagaacca tactatggct tcaaaagact ttcacattgt tgcagaaaca
      361 ggtattcatg cgcgtccagc gactttgctt gttcaaacag ctagcaaatt tgcttcagac
      421 atcactcttg actacaaagg taaagcagta aatctaaaat caatcatggg tgttatgagt
      481 cttggtgttg gtcaaggtgc agatgttact atctcagctg aaggtgcaga tgctgaagat
      541 gccattgcag caattgaaga aacaatgact aaagagggat tggcataaga ctatgacaga
      601 aatgcttaaa ggaattgcag cctcagacgg cgttgctgtt gctaaagcat atctactagt
      661 tcagccggat ttgtcatttg agactgttac agtcgcagat acaaatgcag aagaagctcg
      721 ccttgatgtt gcactccaag ctgcacaaga cgagctttct gttatccgtg aaaatgcagt
      781 agaaagctta ggtgaagaag cagcagccgt ttttgatgcc catttgatgg ttcttgctga
      841 tccagaaatg atcagccagg ttaaagaaac gattcgcgca aaacaaacga atgcagaaac
      901 aggtcttaaa gaagtgactg acatgttcat caccatcttt gaaggcatgg aagataaccc
      961 atacatgcaa gaacgtgcag cggacatccg cgacgttgca aaacgtgtgt tggctcacct
     1021 tttaggtgta aaacttccaa atccagctac aatcaatgaa gaatcaatcg ttatcgcaca
     1081 cgatttgaca ccttcagata ctgctcaact taacaaacaa tttgtaaaag catttgttac
     1141 aaatatcggt ggtcgtacaa gtcactcagc tatcatggca cgtacacttg agatcgctgc
     1201 ggtacttgga acaaatgata ttacaaaacg tgttaaagat ggtgatgtga ttgccgttaa
     1261 tggtatcact ggtgaagtga ttatcgatcc aagcgaagat caagtacttg cttttaaaga
     1321 agctggtgcg gcttatgcca aacaaaaagc agagtggtct ctccttaaag atgcgcacac
     1381 tgaaacagct gatggcaaac actttgaatt ggctgctaat atcggtacgc ctaaagacgt
     1441 tgaaggtgtt aatgacaatg gtgctgaagc tgttggcctt taccgtactg agttcttgta
     1501 catggattct caagacttcc caactgaaga cgaacaatac gaagcttaca aggcagtgct
     1561 tgaaggcatg aatggcaaac ctgttgtggt tcgtacgatg gatattggtg gcgacaagga
     1621 acttccttac tttgaccttc caaaagaaat gaatccattc cttggtttcc gtgctcttcg
     1681 tatttccatc tctgaaactg gggatgccat gttccgcaca caaatgcgtg cgcttcttcg
     1741 tgcctctgtt cacggacaac ttcgtattat gttcccaatg gttgcgcttc ttaaagaatt
     1801 ccgtgctgca aaagcagtct ttgacgaaga aaaagcaaac ttgcttgcag aaggcgttgc
     1861 ggttgctgat gacatccaag ttggtatcat gattgagatt cctgcagctg ctatgcttgc
     1921 agaccaattt gctaaggaag ttgatttctt ctcaattgga acaaacgacc ttatccaata
     1981 cactatggca gcagaccgta tgaacgaaca agtatcatac ctttaccaac catacaaccc
     2041 atcaatatta cgtttgatca acaatgtgat caaagcagcg cacgctgaag gtaaatgggc
     2101 aggtatgtgt ggtgagatgg caggtgacca acaagctgtt ccacttcttg tcggaatggg
     2161 cttggatgag ttttctatgt cagcaacttc agtacttcgt acgcgtagtt taatgaagaa
     2221 acttgactct gctaagatgg aagaatatgc aaatcgtgcg cttacagaat gttcaacagc
     2281 agaagaagtt cttgaacttt ctaaagaata cgtttctgaa gattaaaggg ttaaagagtt
     2341 ggtttaaacc agctcttttt tgctagctaa aaatacaatt actttttggt gtgaaatcat
     2401 ttgagttatc aaataattat cagaattttc agatatttag tatagccaaa tcttgaatta
     2461 tatcggaaaa gtgatagaat aagattacta tcataaaagg agataaaacg ttggcaaaac
     2521 aatataaaaa tttagtgaac ggtgaatgga aactatcaga aaacgagatt accatttacg
     2581 caccagcaac aggtgaagag ttaggatcag ttccagcgat gacgcaggca gaggtagatg
     2641 ctgtttacgc ttcagctaaa aaggctctat cagattggcg cgctttgtct tatgtggaac
     2701 gtgcagctta ccttcataaa gcggctgata ttttagtacg tgatgctgaa aagatcggcg
     2761 cgattctttc aaaagaagta gccaaaggtc acaaggcagc tgtcagtgaa gttattcgta
     2821 ccgctgaaat cattaattat gcagcagaag aagggcttcg tatggaaggt gaagttcttg
     2881 aaggtggtag cttcgaagct gcaagtaaga agaagattgc tattgttcgt cgtgaaccag
     2941 ttggtttagt tcttgccatc tcacctttta attatcccgt taacttggca ggttctaaaa
     3001 ttgctccagc tcttattgca ggaaatgttg ttgctcttaa accaccaaca caaggctcta
     3061 tttctggttt gttactagca gaagcttttg cagaagctgg tattccagca ggtgtcttta
     3121 ataccattac agggcgaggt tctgttatcg gtgattatat cgttgagcac gaagcggtta
     3181 gctttatcaa ctttacaggt tctactccaa ttggggaagg aatcggtaaa ttagcgggta
     3241 tgcgaccaat tatgcttgag cttggcggta aggattctgc tatcgttttg gaagatgcag
     3301 atcttgcttt agcagcgaaa aatattgtag ccggtgcttt tggttactca ggccaacgtt
     3361 gtacagcggt taaacgtgtt cttgtgatgg acaaggtggc ggatcaattg gcggctgaga
     3421 ttaaaacact tgttgaaaaa ctaagtgtcg gaatgcctga agacgatgct gatattacac
     3481 cattaattga tacatcagct gctgattttg ttgaagggtt gattaaagat gcaactgata
     3541 agggagctac tgctttgaca gcctttaatc gtgaaggcaa tcttatttca cccgttctct
     3601 ttgatcatgt gacaactgac atgcgtttgg catgggaaga gccgttcggc ccagtattac
     3661 caattattcg tgtaaccact gtagaagaag ccatcaagat ttctaatgag tctgaatatg
     3721 gtttgcaagc ttctattttt acaactaatt tcccaaaagc ttttggcatt gctgagcaat
     3781 tagaagttgg aactgttcac cttaacaata aaacacaacg tggaacagat aatttcccat
     3841 tcttaggcgc taaaaaatca ggtgcagggg tacaaggagt taaatattct atcgaagcta
     3901 tgacaactgt taaatctgtt gtatttgata tccagtaata aaaaaaataa acaaaaagtt
     3961 aggttaactt aactttttgt ttattt
//
LOCUS       DAXWIQ010000080         3893 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000080 DAXWIQ010000000
VERSION     DAXWIQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.080"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            59..538
                     /gene="rlmH"
                     /locus_tag="ACGEUI_001706"
     CDS             59..538
                     /gene="rlmH"
                     /locus_tag="ACGEUI_001706"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFF8166517.1"
                     /translation="MKVKLICVGKLKERYLKDGISEYQKRLSRFCQFEMIELTDERTP
                     DKASFADNQLIMSKEAQRIHKKIGERDFVIALAIEGKQFPSETFSELISGVTVKGYST
                     ITFIIGGSLGLDSIIKKRANMLMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK"
     gene            complement(695..768)
                     /locus_tag="ACGEUI_001707"
     tRNA            complement(695..768)
                     /locus_tag="ACGEUI_001707"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(732..734),aa:Arg,seq:tct)
     gene            933..1697
                     /locus_tag="ACGEUI_001708"
                     /pseudo
     CDS             933..1697
                     /locus_tag="ACGEUI_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1892..1963
                     /locus_tag="ACGEUI_001709"
     tRNA            1892..1963
                     /locus_tag="ACGEUI_001709"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1925..1927,aa:Glu,seq:ttc)
     gene            1997..2070
                     /locus_tag="ACGEUI_001710"
     tRNA            1997..2070
                     /locus_tag="ACGEUI_001710"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2031..2033,aa:Asn,seq:gtt)
     gene            complement(2567..>3893)
                     /locus_tag="ACGEUI_001711"
     CDS             complement(2567..>3893)
                     /locus_tag="ACGEUI_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFF8166518.1"
                     /translation="LLGYTISLFSFRNSQIPSTFISAFILIFSLLESGLNTYYQLQGI
                     NKEWGFPSRQIYNSQLKDINNLVNSVSKNSQPFFRMERLLPQTGNDSMKFNYYGISQF
                     SSVRNRLSSSLLDRLGFQSKGTNLNLRYQNNTIIMDSLLGIKYNLSEGPPNKFGFTKL
                     KTSGNTTLYQNHYSSPLAILTRNVYKDVNLNVNTLDNQTKLLNQLSGKSLTYFNLQPA
                     QLISGANQFNGQISAQASDYQNSVTLNYQINIPKHSQLYVSIPNIIFSNPDAKEMRIQ
                     TDNHNFIYTTDNAYSFFDLGYFADAKVATFSFVFPKNKQISFKEPHFYSLSIESYLEA
                     MNSIKQKNVHTYAKSNTVITDYNSKTKGSLIFTLPYDKGWSAQKDGKNLPVKKAQGGF
                     LSVTIPKGKGRVILTFIPNGFKLGLSLSCVGIIAYMLLYKYIDIKSKLL"
BASE COUNT         1335 a          610 c          687 g         1261 t
ORIGIN      
        1 aaaaataact tgtggataaa ttgtgtaaaa agtaatgttc ttgtgataca attaagctat
       61 gaaagtgaaa ctaatttgtg ttggaaaact aaaagaaaga tacctcaaag atggtatttc
      121 agaatatcaa aaaagacttt cccgtttttg tcaattcgaa atgattgagt taactgatga
      181 aagaacacca gataaagcaa gttttgctga taatcaattg attatgagta aagaagctca
      241 gcgaatccat aaaaaaattg gggaacgtga ttttgtaatt gcattagcta ttgaaggaaa
      301 acagtttcct tcagagacat ttagtgaatt aatatcaggg gttactgtta agggatattc
      361 aactatcacg tttattattg gtgggagtct tggattagat tcaattatta aaaaaagagc
      421 taatatgcta atgagttttg gattgttaac cttacctcat cagttaatgc gtctagtcct
      481 tactgagcaa atttatcgtg cttttatgat tacacaaggc agtccttatc ataagtaaga
      541 gagtgtgaac tggaataact aactcttacc actttattag ataatagttc ggtataatta
      601 aaagcaaata ttaaaaaaag acgtactaaa tcctatcttt tcaatacact aaggtaaata
      661 caaaaaaagc actaaaacag tgctttttta tctttgatcc cagcaggatt cgaacctgcg
      721 accgttcgct tagaaggcga atgctctatc cagctgagct atgagacctt aaccacatca
      781 ttttaacaaa aattatctaa aactgtcaat atttattttg aaaaaactag aaaataacat
      841 tatatcccgc gttataaaat gaagtacaac aaaaaagaca tgttcgtctg atatactaga
      901 attccccaat tcagtatgga aagcagatga acatgtctaa tattaattct accagaaaat
      961 catcctattc tcatctttca gccacagaac ggggtgaaat tgctgcttat cttaaaatgg
     1021 gaaagaaacc cgttgagatt gctcgacttc tgggtagtca ccgttctaca atctgccgag
     1081 aaataaaacg tggttcagta gatcaggtaa aggataagaa tggaaaacaa accttcttca
     1141 acgcctattt cgctgatagt agacaacgtg tctatgaaac caatagattt gtctaagatg
     1201 gttagtatca gaaaacgttc taagaaagtc atgaagacga ataagaagac tttaggtaaa
     1261 tctatcgaag aacgtccaga atatattaat gatcgttctg aatttgggca ttgggagatt
     1321 gatttggctc tcggcaagaa aaccaaaagt gaagctgtta tgttgacttt ggtagagcgc
     1381 cagacacgct atgcactata gaagacaagc agtcccaaac cattaaccgt gctgtcacgc
     1441 atctcatcag tcagtatcct attgcatcca tcacagcgga taatggttct gagtttagtt
     1501 tactctcaga cttaaaagct gttgacgttt actttgcaca tccctattct tcacatgaga
     1561 gaggaacaaa cgagaacttc aatggtctcc tcagagaata tgtccctaaa ggaatctcac
     1621 ttaatccact aacctctgaa gaacttgaca atgatattac tgccatcaat gagcgcctaa
     1681 gacgacttct tcaatagcaa tcctcaaaat tcctgtttga gctatcccga acagcttaac
     1741 ctctggaact ctagttatct atgaactcgt tgcacttgac ttgacaactt gcggtttaaa
     1801 aaaaatcaaa ttttttttaa aaaagtatag acaaaaaaaa tcaatctgct ataatagtaa
     1861 gagtgttaag gagatatcct ccaaaacttc tggtccgttg gtcaaggggt taagacaccg
     1921 ccttttcacg gcggtaacac gggttcgaat cccgtacgga ctatcgcaat tgtcattttg
     1981 gcaatttttt tctttttccg gcatagctca gttggtagta gcgcatgact gttaatcatg
     2041 atgtcgtagg ttcgagtcct actggcggag tgataagata tttcctaaga taatcttagg
     2101 atttttttat ttagtaaaac agatactcag gtcaaatgct caatttacta attattaaac
     2161 cactaaatac agcattgtga actaacagga ttgtattttg cattaaaata gaagttctgt
     2221 tctttaattg tatagatgat aacattaagt ttaattacaa acattaactg aagcttaatt
     2281 ttttacattt tgaaagcatg tctcccattc aatactgatg aatataagat tattgtaagg
     2341 agtcaaaaga ttgaaatcgt atcagcgatg attattgtca tttcaatctt tgaaagagag
     2401 tagtttaaca ttaaccctca cgaaaaatct gacctttata ttatttgagt ttattagtaa
     2461 aatcgataaa agcatctcaa gtattagaat atttgtctag ctcttgagat acttttagta
     2521 tattgactca tttgccaaat tgataaaaaa atctgatttt ttcggactaa agtaatttag
     2581 actttatatc tatgtactta tacaaaagca tataagcgat aattcctaca caagatagag
     2641 ataaccctaa tttaaaacca ttaggaataa aggtaaggat aacacgtccc tttcctttag
     2701 gaatagtaac tgatagaaat cctccttgtg cttttttgac tggaagattt ttcccatctt
     2761 tttgtgctga ccaaccttta tcgtaaggaa gtgtaaaaat aagagaacct ttcgtttttg
     2821 aattataatc agtgattacc gtattacttt tagcgtaagt atgaacattt ttttgtttaa
     2881 tgctattcat tgcttcaagg taagattcaa tagacaaact ataaaaatga ggttccttaa
     2941 aactaatttg tttatttttt ggaaaaacaa acgaaaatgt agcaactttg gcatcggcga
     3001 aatatcctaa atcaaaaaaa gagtaagcgt tatctgtagt atatatgaaa ttatgattat
     3061 ctgtctgaat acgcatctct ttagcatcag gatttgaaaa tataatattg ggtatgctaa
     3121 catagagttg actatgttta gggatgttaa tttgataatt aagggtaacg gagttttgat
     3181 aatcagaagc ttgtgcagat atttgtccgt taaattgatt agcaccagaa ataagttgag
     3241 ctggctgtaa gttaaaatag gttaaagatt tcccacttag ttggttaagt aatttggttt
     3301 ggttatcaag ggtattgaca tttaggttga catctttgta aacattacgt gttaatatag
     3361 ctaaagggct actatagtga ttttgataaa gagtagtatt cccgctagtt tttagttttg
     3421 taaatccaaa tttatttgga ggtccttcgc taagattata ttttatacca agtagactgt
     3481 ccataataat agtattgttt tggtatctaa ggtttaaatt tgtgccttta gactgaaatc
     3541 ccaatcgatc caataaagaa ctagatagtc tatttcttac agaggaaaat tgtgaaatgc
     3601 cgtaataatt aaatttcatg ctatcgttcc ctgtttgggg aagtagcctt tccattctaa
     3661 aaaaaggttg actatttttt gacacagagt tgacaaggtt gttaatatcc tttaattgac
     3721 tattatatat ctgtcgtgat gggaatcccc actccttatt aattccttga agctggtagt
     3781 aggtgtttaa ccctgattca agaaggctaa agataagtat gaaagcagaa ataaaagtag
     3841 atgggatttg agaatttcta aacgaaaata gtgaaatagt ataacctaat aat
//
LOCUS       DAXWIQ010000081         3826 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000081 DAXWIQ010000000
VERSION     DAXWIQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.081"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            154..1068
                     /gene="hasC"
                     /locus_tag="ACGEUI_001712"
     CDS             154..1068
                     /gene="hasC"
                     /locus_tag="ACGEUI_001712"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     HasC"
                     /protein_id="HFF8166519.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILVVTGKAKRSIEDHFDSNFELEYNLQAKGKNELLKLVDETTAINLHFIR
                     QSHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNASAKPLTKQLMEDYDKTHASTI
                     AVMKVPHEDVSSYGVIAPQGKAVKGLYSVDTFVEKPQPEDAPSDLAIIGRYLLTPEIF
                     GILERQTPGAGNEVQLTDAIDTLNKTQRVFAREFKGNRYDVGDKFGFMKTSIDYALEH
                     PQVKEDLKNYIIKLGKALEKSKVPTHSK"
     gene            1376..1789
                     /gene="yaaA"
                     /locus_tag="ACGEUI_001713"
     CDS             1376..1789
                     /gene="yaaA"
                     /locus_tag="ACGEUI_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFF8166520.1"
                     /translation="MIYKLFTEFITLQALLKELGIIQSGGAIKGFLAETTVLFNGEDE
                     KRRGKKIRVGDKISLPDQDLIITIVEPSQEEKEQFAEEMAEKTRVAALVKQMNQANKK
                     TSSKHNNRQSTTKKSLRATKKTKGKPTAPVRFPGI"
     gene            1791..2897
                     /gene="recF"
                     /locus_tag="ACGEUI_001714"
     CDS             1791..2897
                     /gene="recF"
                     /locus_tag="ACGEUI_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFF8166521.1"
                     /translation="MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIY
                     FLSLTRSHRTRADKELIHFDHSTVSLTGKIQRISGTVDLEINLSDKGRVTKINALKQA
                     KLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
                     NSYLKSAQQIDAAFLAVLDEQLASYGARVMEHRIDFINALEKEANTHHQAISNGLESL
                     SLSYQSSVVFDKKTNIYQQFLHQLEKNHQKDFFRKNTSVGPHRDELAFYINGMNANFA
                     SQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQTKLLETVIKENVQT
                     FITTTSLDHLSQLPEGIRIFHVTKGTVQIDSDIH"
     gene            complement(2952..3815)
                     /locus_tag="ACGEUI_001715"
     CDS             complement(2952..3815)
                     /locus_tag="ACGEUI_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhaT/GlcU family sugar-proton symporter"
                     /protein_id="HFF8166522.1"
                     /translation="MEGIFYALIPMFTWGSIGFVSNKIGGKPSQQTLGMTFGALLFSL
                     AVWLIVRPEMTLQLWLFGILGGFIWSIGQTGQFHAMQYMGVSVANPLSSGSQLVLGSL
                     IGVLVFHEWTRPMQFVVGSLALLLLIVGFYFSSKQDDANAQVNHLHNFSKGFRALTYS
                     TIGYVMYAVLFNNIMKFEVLSVILPMAVGMVLGAITFMSFKISIDQYVIKNSVVGLLW
                     GIGNIFMLLAASKAGLAIAFSFSQLGAIISIVGGILFLGETKTKKEMRWVVTGIICFI
                     VGAILLGVVKS"
BASE COUNT         1326 a          742 c          675 g         1083 t
ORIGIN      
        1 catttttact ttaaaagaac tgcagttggt ggtatgattg agcactatga aatagtcgaa
       61 aaatcatatg aaaacgtaac atgatgtcaa atgaattccg ttctgaaaac gaatgtcact
      121 aagccataga ttataaaaag tgaggagtta ctgatgacca aagtcagaaa agccattatt
      181 cctgctgcag gtctaggaac acgtttttta cctgctacca aagctcttgc caaagagatg
      241 ttgcccatcg ttgataaacc aaccatccag tttatcgtcg aagaagcgct aaaatctggc
      301 atcgaggaaa tccttgtggt gaccggaaaa gctaaacgct ctatcgagga ccattttgat
      361 tcaaactttg aattagaata caacctccaa gctaagggga aaaatgaact gttgaaatta
      421 gtggatgaaa ccactgccat taaccttcat tttatccgtc aaagccaccc aagagggctg
      481 ggagatgctg tcttacaagc caaagccttt gtgggcaatg aaccctttgt ggtcatgctt
      541 ggagatgact taatggacat tacaaatgca tccgctaaac ctctcaccaa acaactcatg
      601 gaggactatg acaagacgca tgcatccact atcgctgtga tgaaagttcc tcatgaagat
      661 gtgtctagct atggggttat cgctcctcaa ggcaaggctg tcaagggcct ttacagtgta
      721 gacacctttg ttgaaaaacc acaaccagaa gatgcgccta gtgatttggc tattattggt
      781 cgttacctcc taacccctga aatttttggt attttggaaa gacagacccc tggagcaggt
      841 aacgaagtgc aactcacaga tgctatcgat accctcaata aaactcagcg tgtctttgca
      901 cgagaattta aaggcaatcg ttacgatgtt ggggataaat ttggattcat gaaaacatct
      961 atcgactatg ccttagaaca cccacaggtc aaagaggact tgaaaaatta cattatcaaa
     1021 ctaggaaaag ctttggaaaa aagtaaagta ccaacacatt caaagtaatg tgttggtact
     1081 ttactttttt ccttgtttat agcaacttaa gtttatatct tgaaaattgt caacgttcca
     1141 aaatataaaa gttttgtgtt atacaacttt actagaggga catattaata ataggattta
     1201 tacgtagaaa aatgataaac ataagtacag cttttgttaa aactaattaa caagaacttc
     1261 aaaactaaat agttatttgg acgagtatga tactttataa atagctctta ttcagctatt
     1321 attatttatt tctgttttat gctataatga cttgaatgac aaaatggaga tgacaatgat
     1381 atacaaatta tttacagaat ttatcaccct acaagccctt cttaaagaat taggaattat
     1441 ccaaagtggt ggcgctatca agggatttct cgctgaaaca accgttctat ttaatggtga
     1501 agatgagaaa cgtcgaggca aaaaaatcag agtaggggat aagatttctc tacctgacca
     1561 agacttaatt atcactattg ttgagcctag tcaagaagaa aaagaacaat ttgccgaaga
     1621 aatggctgaa aagacacgtg tagcggctct agtgaaacaa atgaaccaag ctaacaaaaa
     1681 gacttcttct aaacataaca atcgtcaatc tactaccaaa aaatcactaa gagcaactaa
     1741 gaaaactaaa ggtaaaccaa ctgcacctgt ccgcttccca ggtatctagt atgtggatta
     1801 aagagttaga gttaaaacat tatcgcaatt atgaccacct gcttgcctca ttttcgtcgg
     1861 gtttaaatgt ctttatcggt aataatgctc aaggaaaaac caattttctc gaggcaatct
     1921 attttctatc acttactaga agtcatcgca cgagggctga taaagaattg attcattttg
     1981 atcactcaac tgtttctctt actggtaaga ttcagcgtat tagtggtact gttgatttag
     2041 aaatcaatct atcagataaa ggacgtgtga caaagattaa tgcgctaaag caagcaaaat
     2101 tatctgatta tattggaacg atgatggttg tgctttttgc accagaagat ttgcaactag
     2161 ttaagggagc acctagcctt cgtcgcaaat ttatcgacat tgaccttggt caaattaagc
     2221 ctgtttactt atctgagtta tcccattata accacgtgct taaacagcgt aatagctatt
     2281 taaaaagtgc ccaacaaatt gatgccgctt ttctagctgt tctagatgag caattagcta
     2341 gctatggagc ccgtgtcatg gaacatcgta ttgattttat caatgcgtta gagaaagaag
     2401 caaatactca tcaccaagcc atctcaaatg gtttagaaag tctctctctc tcctatcagt
     2461 cgtcagttgt ctttgacaaa aaaactaata tttaccaaca atttttacac caacttgaaa
     2521 aaaatcatca gaaagatttc ttccgtaaaa acactagcgt tggtccacat cgcgatgaat
     2581 tagcctttta cattaacggg atgaatgcta actttgctag tcaaggtcag caccgcagcc
     2641 ttatcctttc cttaaaaatg gctgaagtta gtttaatgaa ggccttgact ggagataatc
     2701 ccattctttt actagacgat gtcatgagcg agctagacaa tacacgacaa acaaagttac
     2761 ttgaaacagt aattaaagaa aatgtccaaa cctttatcac aacaacaagt ttggatcacc
     2821 tgtcacagtt gccagaaggt atccgtattt ttcacgtgac aaaaggaacc gttcaaattg
     2881 attctgatat tcactaataa catcaaaaaa gggttcagtt aaactgagcc ttttttgata
     2941 ttatgtggcg attaagattt gaccacacct aataaaatag cacctacgat aaaacaaata
     3001 attcctgtga caacccaacg catttctttt ttggttttgg tttcgcctag gaataaaatg
     3061 cctccgacaa tcgaaatgat agcacctaat tgtgagaaac taaaggcaat agcaagtcct
     3121 gcttttgatg cagctaaaag cataaaaata ttcccaatgc cccaaagtaa tccaacaacg
     3181 ctatttttaa tcacatactg gtcaatggaa attttaaatg acatgaaagt tatagctcct
     3241 aaaaccatac ctactgccat gggtaaaatg actgacaaaa cttcaaattt catgatgtta
     3301 ttaaataata cagcatacat cacgtaacca attgttgaat aagtgagcgc cctaaatcct
     3361 tttgaaaaat tatgaaggtg attgacttgt gcattagcat catcttgttt actagaaaag
     3421 taaaaaccaa caatcagaag taatagtgct agacttccta ctacaaattg cataggtctt
     3481 gtccattcat gaaaaacgag gacaccaatc aaacttccca gaacaagttg agagccactt
     3541 gacaaaggat tggcaacgga gactcccatg tattgcatgg catgaaattg accagtttga
     3601 ccaattgacc agataaaacc acccaatatt ccaaaaagcc aaagttgcag tgtcatctca
     3661 ggtctaacaa ttaaccaaac agctaatgaa aataataaag ccccaaaagt cattcctaga
     3721 gtttgttgcg aaggttttcc tccaattttg ttactaacaa atccaatgct tccccatgta
     3781 aacatgggaa taagtgcata aaaaatacct tccaaaatgt tatctc
//
LOCUS       DAXWIQ010000082         3597 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000082 DAXWIQ010000000
VERSION     DAXWIQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.082"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..1913
                     /gene="ftsH"
                     /locus_tag="ACGEUI_001716"
     CDS             <1..1913
                     /gene="ftsH"
                     /locus_tag="ACGEUI_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFF8166523.1"
                     /translation="ITGFQFYLKGTSTQSQQISYSKLIKHLKAGDIKSLSYQPSGSII
                     EVKGKYEKPQKVTVNSGLSFLGGRASTQVTEFSSLVLPSDTILKEMTAAADKNGTELT
                     VKQESSSGTWITFLMSFLPIVIFAAFMMMMMNQGGGGARGAMSFGKNKAKSQSKGNVK
                     VRFTDVAGAEEEKQELVEVVDFLKNPKKYKSLGARIPAGVLLEGPPGTGKTLLAKAVA
                     GEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAKKAERAIIFIDEIDAVGRRRGAGM
                     GGGNDEREQTLNQLLIEMDGFEGNENIIVIAATNRSDVLDPALLRPGRFDRKVLVGRP
                     DVKGREAILRVHAKNKPLANDVNLKVVAQQTPGFVGADLENVLNEAALVAARRNKIKI
                     DASDIDEAEDRVIAGPSKKDRTISQKEREMVAYHEAGHTIVGLVLSNARVVHKVTIVP
                     RGRAGGYMIALPKEDQMLLSKEDLKEQLAGLMGGRVAEEIVFNAQTSGASNDFEQATQ
                     IARAMVTEYGMSEKLGPVQYEGNHAMMPGQISPEKAYSAQTAQMIDDEVRELLNQARN
                     QAADIINENRDTHKLIAEALLKYETLDAAQIKSIYETGKMPVDLETDDNEAHALSYDE
                     IKNKMTESESAE"
     gene            2238..>3597
                     /locus_tag="ACGEUI_001717"
     CDS             2238..>3597
                     /locus_tag="ACGEUI_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8166524.1"
                     /translation="MTIFRKKKKYSNKTEMQRHFKVIDLVFLGLGSMVGTGIFTVTGI
                     GAAKYAGPALTISIIISAIAIGILALFYAEFASRMPSNGGAYSYVYATLGEFPAWLVG
                     WYIIMEFLTAISSVAVGWGSYLKGLLANYNIVLPNALNGTFNLKNGTYIDILPVLVMF
                     FVTGIVLMNSKLALRFNSFLVILKFSALALFIFVGIFFIDHNNWSHFAPYGVGQITGG
                     KTGIFAGASVMFFAFLGFESISMAVDEVKEPQKTIPKGIILSLIIVTALYIVVTTILT
                     GIVHYTKLNVPDAVAFALRNIRLYWAADYVSIVAILTLITVCISMTYALARTIYSISR
                     DGLLPKSLYTLTKKNKVPQNATLVTGLLAMICAGIFPLSSLAEFVNICTLAYLIILSG
                     AIIKLRRIEGEPKANEFKTPLVPFLPMLAIIICLSFMSQYKAFTWIAFAIATIIGTLI
                     YLA"
BASE COUNT         1142 a          552 c          725 g         1178 t
ORIGIN      
        1 tcatcacagg atttcaattt tatcttaagg gaaccagcac acaaagccaa cagattagtt
       61 attccaaatt aattaaacat cttaaggctg gagatataaa atctttaagt tatcagccta
      121 gcggtagtat tattgaagtt aaagggaaat atgagaaacc tcaaaaagtg acagttaatt
      181 caggactatc atttttaggt ggaagagctt caacacaggt aacagagttc tcatctttag
      241 tattacctag tgataccatc ttgaaagaaa tgacagcagc agctgataaa aatggaacag
      301 agcttacagt taagcaggaa agttcaagtg gtacttggat taccttctta atgagctttt
      361 tacctattgt tatttttgct gctttcatga tgatgatgat gaatcaaggt ggtggaggag
      421 ctcgtggtgc tatgagtttc ggcaaaaata aggcaaaatc tcaatcaaaa ggtaatgtta
      481 aagtgagatt tacagacgtt gctggtgccg aagaagaaaa acaagaatta gtagaagttg
      541 ttgatttttt gaaaaatcct aaaaaatata aatcactagg agcacggatt ccagcgggag
      601 ttcttctaga ggggcctcca ggtactggta aaacattact agctaaagct gtagcaggtg
      661 aagctggagt gccatttttc agtatttcag gttcggattt tgtcgaaatg tttgtcggtg
      721 taggtgctag tcgtgttcgt tctttatttg aggatgctaa aaaagctgag cgtgcgatca
      781 tctttatcga tgaaattgac gctgtaggtc gtcgtcgtgg tgctggcatg ggaggcggta
      841 acgatgaacg agaacagacc cttaaccaat tactcattga aatggatggt tttgagggta
      901 atgagaacat tatcgttatt gctgcaacta accgtagtga tgttttagat ccagctttac
      961 taagaccagg tcgttttgat cgtaaggttt tagttggtcg ccctgatgtt aaaggacgtg
     1021 aagccatttt acgtgttcat gctaaaaata aaccacttgc aaatgacgtt aatttgaaag
     1081 tagtagccca acagactcca ggttttgtag gtgctgatct tgaaaatgtt ttaaatgaag
     1141 ctgcgttggt tgctgctcgc agaaataaaa ttaaaattga tgctagtgat attgatgaag
     1201 ctgaagatcg tgttattgct ggaccatcaa agaaagatcg cactatttcg caaaaagagc
     1261 gagaaatggt tgcttatcac gaagctggtc atactattgt tggtttagtt ttatctaatg
     1321 cgcgtgtagt tcacaaggta actattgtac ctcgtggacg agcaggtggt tatatgatcg
     1381 ctcttccaaa agaagatcaa atgcttcttt ctaaagaaga tctgaaagag caattagcag
     1441 gattgatggg tggtcgtgtt gctgaagaaa ttgtttttaa tgctcaaaca tcaggtgcgt
     1501 caaatgactt tgagcaagct actcagatag caagggccat ggttacggaa tatggtatga
     1561 gtgaaaagct tggaccagtt caatatgaag gtaaccatgc gatgatgcca ggacagatct
     1621 ctcctgaaaa agcttactct gcccaaacag ctcaaatgat tgatgatgag gttcgtgaac
     1681 tcttaaatca agctcgtaat caagcagctg atatcatcaa tgagaatcga gatactcata
     1741 aattgattgc tgaagctctt ctcaaatatg aaactctaga cgctgcacaa attaaatcaa
     1801 tttatgaaac tggtaaaatg ccagttgatt tagaaactga tgataatgaa gctcatgcgt
     1861 tatcttatga cgaaattaaa aataaaatga cagaatctga atctgcagaa tgatataaaa
     1921 aagctgaact tatagattaa gttcagcttt tttatcaaca caatatcctt gtaaacgtta
     1981 tgaattgtaa taggtgccta attgctagaa atgagaagat gacatacttt ttttctttat
     2041 tacttaaagt gagaaagact ttggtattta aaactagaaa attgttaaag ccatttgaaa
     2101 aatctattag tgttttctta gaataataga agaacagaag atagcaaaag tttcagtcaa
     2161 ccaataagat gaaatttaca atcattacta actatgatac acttagtaga aaatcattta
     2221 ctatagaagg tgataacatg actatttttc gtaaaaaaaa gaaatattcc aataaaacag
     2281 aaatgcaaag gcacttcaaa gttattgatt tagttttttt aggcctaggt tcaatggttg
     2341 gaacaggtat ctttacagtt actggcatag gcgctgctaa gtatgcaggt cctgctttaa
     2401 caatatcgat tatcatttcc gcgattgcta ttggaatttt agccttattt tatgcagaat
     2461 ttgcgtctcg tatgccttct aacggtggtg cttatagtta tgtttatgca accttgggag
     2521 agtttccagc atggcttgtt ggctggtata ttattatgga atttttaaca gctatatcta
     2581 gtgttgctgt tggctgggga agttatttaa aaggattact tgccaattat aatatagtat
     2641 taccaaacgc tttaaatggc acgttcaatc ttaagaatgg aacttacata gatattcttc
     2701 ctgttttagt gatgtttttc gtgacaggga ttgtcctaat gaattcgaaa ttagccctga
     2761 gattcaatag ttttttagtt attttaaaat tttcagcact ggctttattt atctttgtag
     2821 gcattttttt tattgatcac aataattggt ctcattttgc tccatatggt gtaggacaaa
     2881 ttactggagg taaaacaggt atctttgctg gtgcttctgt tatgttcttt gcatttttag
     2941 gatttgaatc tatttcaatg gcagttgatg aagtgaaaga accacaaaaa acaatcccta
     3001 aaggcatcat tttatctttg attattgtaa cagcactcta tatagttgtc accactattt
     3061 taacgggaat tgtacactac actaaattaa atgttccaga cgcggtggct tttgctttaa
     3121 gaaatattag actttattgg gcagctgact atgtgtcgat agttgctatt ttgactctta
     3181 ttacggtatg tatttctatg acctatgcat tagctagaac aatttacagt attagtagag
     3241 atggtttatt acctaaatca ctttatactc tcactaaaaa aaataaagtg cctcaaaatg
     3301 ctactcttgt cactggacta cttgctatga tttgtgcggg aatatttcct ttatcgagtc
     3361 tagcagagtt tgttaatatt tgtactttag catatttaat tattctgtcg ggtgcaatta
     3421 ttaaactcag aagaatagaa ggagaaccaa aagcaaatga atttaaaact cctctagtac
     3481 cattcctacc aatgttggca attatcattt gtctatcatt tatgagccaa tataaagctt
     3541 ttacttggat tgcttttgct attgccacaa ttattggaac attaatctac ttagctt
//
LOCUS       DAXWIQ010000083         3473 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000083 DAXWIQ010000000
VERSION     DAXWIQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.083"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(52..807)
                     /gene="recO"
                     /locus_tag="ACGEUI_001718"
     CDS             complement(52..807)
                     /gene="recO"
                     /locus_tag="ACGEUI_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFF8166525.1"
                     /translation="MQLTESLGIVLFNRNYREDDKLVKIFTEVAGKQMFFVKHISRSK
                     MSSIIQPLTIADFIFKLNDTGLSYVVDYSNVNTYRYINNDIFRLAYASYVLALADAAI
                     ADNESDSHLFTFLKKTLDLMEEGLDYEILTNIFEIQILDRFGISLNFHECAICHRTDL
                     PLDFSHRFSAVLCSEHYYKDNRRNHLDPNVIYLLSRFQKITFDDLRTISLNKDIKKKL
                     RQFIDELYHDYVGIKLKSKTFIDNLVKWGDIMK"
     gene            complement(993..1955)
                     /locus_tag="ACGEUI_001719"
     CDS             complement(993..1955)
                     /locus_tag="ACGEUI_001719"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFF8166526.1"
                     /translation="MSYSDLKLFALSSNKELAEKVASAMGIQLGKSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAEKISVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRH
                     GLVGEDVVVVSPDHGGVTRARKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVL
                     DTIYLPKERLIDKIEQISIADLVAEAIIRIHEKRPLSPLFEIGN"
     gene            complement(2208..3404)
                     /gene="pcsB"
                     /locus_tag="ACGEUI_001720"
     CDS             complement(2208..3404)
                     /gene="pcsB"
                     /locus_tag="ACGEUI_001720"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan hydrolase PcsB"
                     /protein_id="HFF8166527.1"
                     /translation="MKKRILSAVLVSGVTLGAATTVGAEDLSTKIAKQDSIISNLTTE
                     QKAAQNQVSALQAQVSSLQSEQDKLTARNTELEALSKRFEQEIKALTSQIVARNEKLK
                     NQARSAYKNNETSGYINALLNSKSISDVVNRLVAINRAVSANAKLLEQQKADKVSLEE
                     KQAANQTAINTIAANMAMAEENQNTLRTQQANLVAATANLALQLASATEDKANLVAQK
                     EAAEKAAAEALAQEQAAKVKAQEQAAQQAASVEAAKSAITPAPQATPAAQSSNAIEPA
                     ALTAPAAPSAGPQTSYDSSNTYPVGQCTWGAKSLAPWAGNNWGNGGQWAYSAQAAGYR
                     TGSTPMVGAIAVWNDGGYGHVAVVVEVQSASSIRVMESNYSGRQYIADHRGWFNPTGV
                     TFIYPH"
BASE COUNT         1102 a          698 c          557 g         1116 t
ORIGIN      
        1 ttttattata gcatattctg aaaccaattc ccataaacga aagagaccct atcatttcat
       61 aatatctccc cacttaacta aattatcaat gaatgtttta ctttttaatt tgattcctac
      121 atagtcgtga tacaactcat caatgaactg acgaagcttc tttttgatgt ctttattcaa
      181 tgaaatagtt ctcaaatcat caaatgtgat tttttgaaat cgactcaaca agtagataac
      241 atttggatct aaatgattac gtcggttgtc tttgtaataa tgttcagaac aaagtacagc
      301 tgaaaaacga tgggaaaaat caagcggtaa atcagtgcga tgacaaatgg cacactcatg
      361 aaagtttaga ctaataccaa aacgatctaa tatctgaatt tcaaaaatat ttgtcaaaat
      421 ttcataatct aggccctctt ccatcaaatc aagtgttttt ttcaaaaacg taaacaaatg
      481 cgaatctgat tcattatctg caatcgcagc atcagctaat gctaagacat aactagcata
      541 ggctaatcga aaaatatcat tattaatata ccgataagtg ttaacgttac tatagtcaac
      601 aacataagac aagcctgtat catttaactt gaaaataaaa tcagcaatcg ttagtggttg
      661 aatgattgag gacattttgg aacgactaat atgtttcacg aaaaacatct gcttacctgc
      721 tacttcagta aatattttga ctaatttatc atcttctcga taattcctat taaaaagaac
      781 gatgcctagt gattctgtta gttgcatatt acctcgtcgt atttttctct taccttttat
      841 cttagtttac aataaactgt tgttatcttt cttattatac ccaaaatcct ttagaattgc
      901 atagttcatc ccacaataat taataccaca ttacccttac attttattca agtcaaaaag
      961 cccgagatct attatctcag gcttcgttaa gtttaattgc ctatctcaaa taaaggtgac
     1021 aacggacgtt tttcatgaat acgaataatc gcttctgcaa ccaaatcagc aattgaaatt
     1081 tgttcaattt tatcaataag gcgttctttt ggcagataaa tagtatcaag aacaattagt
     1141 ttttcgatag ccgaacgttg aatattatca agtgctggtc cagaaagaac gggatgcgta
     1201 catgacgcat acacagctgt tgcccccgct tcagcaagcg catctgctgc atggcaaata
     1261 gtccctgcgg tatcaatcat atcgtcaatc aagatacatt tcttccccga aacatttccg
     1321 ataatattca tcacttcact ggtgttcatc ttatccacac tacgacgctt atcgataatt
     1381 gcaattggtg tttggaggaa ttgtgctaat ttacgcgcac gagtcacacc accatggtca
     1441 ggactaacaa cgacaacatc ttcacccact aaaccatgac gatcaaaata gtctgcaatt
     1501 aaaggtgcac ccatcaagtg atcaactgga atatcaaaga acccttgaat ttgagcagca
     1561 tgtaaatcaa ccgttaataa gcgatctaca ccagcaactt ctaacatatt agcaactaat
     1621 tttgaagtaa taggttcacg agaacgcgct ttacggtctt ggcgtgcata gccgtagtaa
     1681 ggcatcacaa ctgaaatttt ttcagcacta gcacgcttta aagcatcgac cataatcaaa
     1741 atttccatta aattatcatt gacaggtgag ctagttgatt gcaaaataaa gacatgatga
     1801 ccacgaattg attcttcaat atttacttgg atttcaccat ctgaaaactg gcgaaccgta
     1861 gactttccaa gttgaatacc cattgcagac gcaacttttt ctgctaattc cttgtttgac
     1921 gataaagcaa acaattttaa atcagaataa gacatttttt cctccaaagt tttaatccta
     1981 taccatttta acgcttttca agtgaattat caaatgaaat tgaaaaaccg ttaaagaatg
     2041 tattaaatat accttttatc gactttatat acttctctta tattatacat aagcgattgg
     2101 ccaaacaact ctcttataaa ttaactatat cgtcaatact ctcagaacta ttccacaata
     2161 ggagtaatca aaaaggttag aacaactgtt ctaacctaaa taatatgtta gtgtggataa
     2221 ataaatgtaa cacctgttgg attaaaccag ccacggtggt cagcaatgta ctgtctacca
     2281 ctgtagttag actccatcac acgaatactt gaggcacttt gaacctcaac tacaacggcg
     2341 acatgtccat aaccaccatc gttccaaacg gcaatcgcac ctaccatcgg cgttgaacca
     2401 gtacgataac cagctgcttg agcactataa gcccattgac caccatttcc ccaattattt
     2461 cctgcccaag gagctaaaga tttagctccc catgtgcatt gtccaactgg ataagtatta
     2521 gaagaatcat atgatgtttg tggtcctgca gaaggagctg ccggagccgt gagtgcagct
     2581 ggttcaatag cattactact ttgcgctgcc ggagtagctt gtggtgctgg agtaatagca
     2641 gattttgctg cttcaacaga tgctgcttgt tgtgcagcct gttcttgtgc cttaacttta
     2701 gcagcctgtt cttgtgctaa ggcttcagca gcagcttttt ctgcagcttc tttttgagct
     2761 accaaattag ctttatcttc agtagcagat gctaattgga gagctaaatt tgcagttgca
     2821 gctaccaaat tagcttgttg agtacgtaat gtattttggt tttcttcagc cattgccata
     2881 ttagcggcaa tggtattaat agctgtttgg ttagcagctt gcttttcttc aagggaaact
     2941 ttatcagctt tttgttgttc taacaattta gcgttagcag agacagctct attaattgct
     3001 actaaacggt ttacaacatc agaaattgat ttagaattca aaagtgcatt aatataacca
     3061 gaagtttcat tgtttttata agcactacga gcttgatttt ttaatttttc attacgagca
     3121 acaatttgac ttgttagagc cttaatttct tgctcaaatc gctttgaaag cgcctcaagt
     3181 tctgtatttc ttgcggtcag tttatcttgt tcagattgta gtgaacttac ttgagcctgt
     3241 aacgctgaaa cttgattctg tgcagctttt tgctctgtag tcagatttga gataatagaa
     3301 tcttgcttag caatcttagt acttaaatcc tccgctccta cagttgtagc tgctccgagg
     3361 gtaacaccac ttacaagaac tgctgataaa attctttttt tcataataaa aaaactactc
     3421 cttttcgata agacatctta tattttacca aaaaaaaaag tatttaatat tac
//
LOCUS       DAXWIQ010000084         3243 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000084 DAXWIQ010000000
VERSION     DAXWIQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.084"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            1..903
                     /locus_tag="ACGEUI_001721"
                     /pseudo
     CDS             1..903
                     /locus_tag="ACGEUI_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            903..1733
                     /locus_tag="ACGEUI_001722"
                     /pseudo
     CDS             903..1733
                     /locus_tag="ACGEUI_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1730..2332
                     /locus_tag="ACGEUI_001723"
     CDS             1730..2332
                     /locus_tag="ACGEUI_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166528.1"
                     /translation="MTTKTYFMRSFNLILYLLLIAPICYVFGWLFNLVSVQLSTVTFV
                     NLDTVKNVTDTISSVCYGFALISLLLALLSGLKMIQRLNTDSLGNYCQSIYRTFLLRH
                     FLFQRERVQKVSNFEHQTVTTINPIHNGFNRAVRKCIVDIRKDAITVFLKVPRDQQGQ
                     KILKDTGVQLKEEIVIQHTDYYFSSPVRVRNQLWFIGQKR"
     gene            complement(2808..3092)
                     /locus_tag="ACGEUI_001724"
     CDS             complement(2808..3092)
                     /locus_tag="ACGEUI_001724"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8166529.1"
                     /translation="MMLSKSLQVPLEQLIAEDIPKMTLIIKDEEIKQCDRNTHIMLFG
                     TLLIAISIYPIMSWLGWWGYGPVAILWLIIMRIALRIERFKKTYDVQTYR"
     gene            complement(3096..3140)
                     /locus_tag="ACGEUI_001725"
                     /pseudo
     CDS             complement(3096..3140)
                     /locus_tag="ACGEUI_001725"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1081 a          607 c          530 g         1025 t
ORIGIN      
        1 tttgaagaaa attattcaca agaaatacct actcgtatca aaaatttaag aaaaaaacac
       61 aatattacac aaagcgaact aggcaatgca ggtcaagtta gtcaagttga aagtggtaag
      121 cgaccaatta cgagttcgat gttagtttat ttaaatgctc taaccgcttc gaactatacg
      181 tatatcgttt ttggagagtt agatgagttt attgagaatc tgtttcatta ttttttcagt
      241 tctattttat atagagattt agaggctgta gatgaaaacc tatattcttt catgagtgat
      301 gatttaattt caatccaatc aagttgtcta agtattgcca agacatttgc aaatttcaat
      361 attcaaagaa aaagatttat gatttctact gaaactgaaa tggatacttt tcacaagaaa
      421 gatgacattg acgtatgggt tggtggaaaa agttataacc ccgctaggag ttttaggaat
      481 aatccaataa atgaattaac tgttattgat tttgaagaaa tggctgatat tcttttgttg
      541 atgttgagag ataatctaat taggtcgttt gaaattaatg tctgcaacac actgttcgaa
      601 ttagataaaa atggtgaacc gacaacattt aatctagata aaattgactc aataatcaat
      661 aagtggtggt ctgagaatgt ctcaacagag ataataccca acctcataaa aaaacttaga
      721 gaaaatccct tgtttaatat cggatttatg gtaaatgata tattagaacg aatgtataaa
      781 gaggacatac caaagtctta ccttacttct gttccattag ttatttccaa aaaagcaagg
      841 tcaactttat cgattaagtc aaacggaagt caaaaaatag ataaatcaaa atttgcacag
      901 attttgtccc tttagagacg gatgacttac acattcaacg tggattattc ttcccttgga
      961 gaaaggggat ttcagaacgc ttggcgattc ctgatttaga taaattttca tctaacttac
     1021 aacaggggaa aattcctgtc ataaagcact tcgaactcaa tttagataag gggagtcact
     1081 gggctattgc tggaaattca ggttctggga aaccgtacgc tctcacttac ttcttgagtg
     1141 tcctgaaacc taagtccggc ctaattatca ttgacccaaa attcgatacc cctagccaat
     1201 gggcaagaga aaataaaatt gccgttatcc acccagtaga aaaccattca aaatctgatt
     1261 tcgtttcaca agtcaatgaa caattaaacc agtgtgcaac tctcattcag aaacgacaag
     1321 ctatcttgta tgataatccc aatcatcaat ttacccatct aactattgtt attgatgaag
     1381 tcctcgctct atcagaggga gtcaataaga atatcaaaga agcttttttc tccctactat
     1441 cacaaattgc cttgttggga catgctacta aaatccatct attcttggga agccagcgtt
     1501 ttgaccataa cactattccc atttcagtga gggaacagct aaacgtatta ttgcaaattg
     1561 gaaatatcaa tcagaaaacc actcaattct tatttccaga cctagatcca gagggaattg
     1621 tcattccaac aggacacgga acaggtatca ttcaagttgt cgataatgaa cactcctatc
     1681 aagttctacc cttactctgt ccaacctact atactaagcg aggtatccta tgaccactaa
     1741 aacctacttc atgagaagct tcaatctcat attatactta ctgcttattg ctccaatctg
     1801 ctatgtgttc ggttggctgt tcaatcttgt ttcagttcaa ttatctaccg tcacatttgt
     1861 caatctggat acggttaaaa acgttactga taccattagt tctgtatgtt atggattcgc
     1921 tcttatttct ttacttctag ccttattatc tggattaaag atgatacaac gcttgaatac
     1981 agatagcctt gggaactact gccaatctat ttatcgcacc tttctattaa gacacttcct
     2041 tttccagcgt gaacgagtac agaaggtaag caactttgaa caccagaccg ttaccactat
     2101 caatccaatc cataacgggt ttaaccgagc tgtacgtaag tgtatcgtag atatacgaaa
     2161 agatgccatt actgtatttc taaaagttcc tagagaccaa caggggcaaa aaatcctaaa
     2221 ggatacggga gtacaactga aagaagagat tgtcatccaa cacacagact attatttctc
     2281 ttctcctgtt cgagttcgta accaattgtg gtttattggt caaaagcgtt aattccttgg
     2341 ggctgtggct tgcgttaagc aaaagccaga cagccccttt tttatattct tatctatcat
     2401 gacctattgg ggttactacg acaacgaaag ttagaagctg tgggtattta tttatcttga
     2461 aaagattgct gaactaacgc aataactact ctaccattca ctctaccttt tattaagcaa
     2521 ctatcaataa atattgatac aataaggttt ataacagcta aaatagaaaa tacttaacct
     2581 ttacagaagt aaaataattg agatgagaaa agcctatgaa atgaacgttt caaaggcttt
     2641 tttcgtgttt aaaaaaacaa atgaaaaaac aaaatagaga gtagggttat taacactgtc
     2701 agtcctgtgt acgcaataac aatccatgct ttttgataag gatattttgc tttttcttta
     2761 atcttatcaa tctgatctaa gctctcaccc tttgctatag cttcaagtta tcggtaagtc
     2821 tgaacatcat atgtcttttt aaatcgctca attcttaagg cgatacgcat gattatcaac
     2881 cataagatag caaccggacc atatccccac caacctaacc aagacattat aggataaata
     2941 ctgatagcaa ttaggagtgt cccaaataac ataatatgcg tattcctatc acattgttta
     3001 atctcctcat ccttaataat taaagtcatt tttgggatat cttctgctat tagttgctct
     3061 aaaggaacct gtaaggactt acttaacatc aaaagatttt gcaggtctgg atagctttta
     3121 ccgttttccc agtttgagat agtcttcttg aaacatacat catttcagca agctcttctt
     3181 gtgacatctg taattttttt caataataag ataactggtt tttaaagccc ataataaatc
     3241 tcc
//
LOCUS       DAXWIQ010000085         2632 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000085 DAXWIQ010000000
VERSION     DAXWIQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.085"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            19..929
                     /locus_tag="ACGEUI_001726"
                     /pseudo
     CDS             19..929
                     /locus_tag="ACGEUI_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993827.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(997..2403)
                     /locus_tag="ACGEUI_001727"
     CDS             complement(997..2403)
                     /locus_tag="ACGEUI_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFF8166530.1"
                     /translation="MNNIKTKKRFMDRYIDGFMKWMPESLFICFILTFLVVTMSVLMT
                     DSPFIGTEKTGGIIYGWVNGFWGLLSFAMQMTILLATGNAVASSPPAHKMFKSLAKLP
                     QTRTQIFIFSIVVGSIFGFLHWGLGMMVAIVFGKELLVQARQKGIKVHTPLFVATLFF
                     TFLPATSGLSGAAVLYSATPDYLRNSVADAYKQVVPESVPLTESVLNLPFISLLVVCM
                     LEPLCFALLAHPKDETKIMELDDEIYHHSLDTASHVVIARNTPAEKMNASRLVMYLVG
                     GAIVSYSLYHFSVVGLSGLDLNCFNFLFLGLGLLLCGQQGPEYYGSLFKDGVMSSWGL
                     VLQFPFYAGIFGIIQSTGLGLEISHFFVAISNGTTWPVFAYLYSALLNIAVPSGGSKF
                     VIEAPYIVPATIEVGNDLGKILQAYQLGDATTNLIVPFWALSYLSNFKLKFNQIVAYT
                     IPCVLVVTGIAIIYLFVF"
BASE COUNT          913 a          554 c          442 g          723 t
ORIGIN      
        1 ttatcgaaag aggaaaatat gaatatctca agcttggctt attttattga gattgttaat
       61 tgtgacttta acttaaccaa agcagcccaa aaaagtcata tttcgcaacc cgcccttagt
      121 agttatatca agactatcga aaaagaagaa ggagtcgttt tattccagcg tcaaaaaggg
      181 cgtctagttg gactaacttc agtcggcgaa cgcttttttg aaaatgctaa ggtggttatc
      241 gcatcacatg agcaattaat ggctgatttg cggagtgaat ccaaaaaata caaaggagaa
      301 atttcaatcg gtattcctca actcatctct ggcattgttt tttctgagct cattccaaaa
      361 ctcatcgaag aaaatcccga agttaaattt catattgtag aaacgggtgc ttatgaactg
      421 caaaaaaact gtttctaaaa gaagtcgatt ttgcgatttt attaacaccc actacactag
      481 atgctactat tgctgatatc gacattctcc taagagatca actctgcctt tatatgagtc
      541 acaaaacctt tcaagagacg tttgatagta atcctaacat ctctatcaaa gcattacatc
      601 gtcaaaactt agtaactttt gataaaacct ttatgattaa tcatcagctt accaccttat
      661 ttgaagaagc aaatagtctt cctgtagtca aatgttattc tgcctcgtgg gattttttat
      721 taaattgcac gcgctattcc tcttacctaa caatcctacc aagacctatt actcattttg
      781 ctcatatgga cggtttagtg gaggtccaac tgaccgaaca tccaaaatgg gaagttgttc
      841 tagcctctct caagcacaac aaaacaagcc atttaaaaca ttatatcaag cacaccatct
      901 tagattattt catgaaccag cgcttctaat tgctggactc agaccttaaa aaaacgcctg
      961 aaggaaacaa tcccaagcgt ttcatgtagt tatttcctaa aatacaaata ggtaaatgat
     1021 ggcaatacct gtgaccacca agacacaagg aatagtataa gccacaattt gattgaattt
     1081 tagtttgaag tttgagaggt aagataaggc ccaaaaagga actatcaagt ttgttgtggc
     1141 atctcctaat tgataagcct gcaaaatctt acctaagtca ttaccaactt cgatagttgc
     1201 agggacgata tagggagctt caatcacaaa ttttgagcct cctgatggta ccgcaatatt
     1261 cagcaaagcc gagtaaagat aagcaaagac cggccatgtt gtcccatttg aaatagcgac
     1321 aaagaaatgg gaaatctcta atcctaaacc tgtactttga atgattccaa aaataccagc
     1381 gtaaaatgga aactgaagca ccaagcccca agatgacatg accccatctt tgaataagga
     1441 accataatat tctggtcctt gttgaccaca aagaagcaag cctaagccta aaaataaaaa
     1501 gttaaaacaa ttgagatcta gaccagacaa gcccaccacc gaaaaatgat aaaggctata
     1561 gctaacaatg gccccaccaa caagatacat cactaagcga gatgcgttca tcttttcagc
     1621 aggtgtgttt cttgcaataa caacatgtga agcggtatca agactatggt gatagatctc
     1681 atcatcaagt tccatgattt tagtttcatc ttttgggtgc gccaataaag caaagcaaag
     1741 cggttccagc atacacacca ctaaaagact gataaatgga agattcaaaa ctgattctgt
     1801 cagaggaaca ctttcaggaa caacctgttt ataagcatct gcgacactat ttcgcaaata
     1861 atctggagta gccgaataaa gcaccgcagc accagataga ccagaagtag ctggtaaaaa
     1921 ggtaaaaaat aaagtagcaa caaacaaagg cgtatgcacc ttaatccctt tttgcctagc
     1981 ctgaacaagc aactctttcc caaacacaat agcaaccatc ataccaagac cccagtgtaa
     2041 aaagccaaag atagagccta ctacaatgga aaagataaaa atctgagtcc tggtttgagg
     2101 cagtttggca agggacttaa acatcttatg agcaggtgga gaactcgcta cagcattccc
     2161 tgtcgcaagt aaaatagtca tctgcatggc aaaggacaac agcccccaaa aaccatttac
     2221 ccagccatag ataatcccgc ccgtcttttc cgtaccaata aagggactat ccgtcatcaa
     2281 cacagacata gtaacgacta aaaaggttaa aataaaacag ataaaaagcg attctggcat
     2341 ccatttcata aagccatcaa tatagcgatc cataaacctt tttttggttt tgatgttatt
     2401 catgttgtca attcctttca cattgtccaa aagcactttt gttcttttga ccagtaacct
     2461 gtcgataata ttaataagcg tttctttagt tcatttagtt atcaataagt ttaaatccac
     2521 aaaaaacact tttgtcccct ttttattatg acagcgtttt cataaacaac tatgattttt
     2581 ttattgcttc cataaccctt atttatagca aaaagaatgg cgcttcactc at
//
LOCUS       DAXWIQ010000086         2542 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000086 DAXWIQ010000000
VERSION     DAXWIQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.086"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..188
                     /locus_tag="ACGEUI_001728"
     CDS             <1..188
                     /locus_tag="ACGEUI_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HFF8166531.1"
                     /translation="LGIHYVELGKGIIDYKTIIHEIHEHFIDLIFSLEVHEKEIDKKA
                     EVVRKSYLTLNEYINGG"
     gene            190..924
                     /gene="cofE"
                     /locus_tag="ACGEUI_001729"
     CDS             190..924
                     /gene="cofE"
                     /locus_tag="ACGEUI_001729"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HFF8166532.1"
                     /translation="MNLSIFGLKNIPYLKEGDSIEKLIEESIKTSEFFIEDNDVLCIA
                     SKVVSIAEGQVMSLNEIQVSDVAKEIHRNIPRKDPRIIEIMLNLVNRDLSRLDIKKNY
                     IGCRLENGLKLTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSD
                     GREDKRGATQVAIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGT
                     GIPAVLIRGLDYKWSENTSIKEALNE"
     gene            917..1903
                     /locus_tag="ACGEUI_001730"
     CDS             917..1903
                     /locus_tag="ACGEUI_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFF8166533.1"
                     /translation="MSRNQDCKNILIIGFGKIGKIKAYKWLSRGYEVYVKDLKFSSMK
                     YLNTNSDVLDDLSRQYFTIEICTPTNHHLTLLKTVITRYVYSYISIEKPLCNRLEDLE
                     EMKSLVESHPHLAKKIFASEQYFFSKILEKLLQVDCFSLDKIKEITVDFSKDRIEDNE
                     NGRFLDKEILGYGIEIPHIIAVISYLGISLEEFKKGIFVNAIYIKDLEKHDYSIEILS
                     CINQTVIKIISNLGSFAIREGKIEGGDAKTVRRVTIDDKTILFDPHPKLERYKSELVT
                     GTNTFQIHDDMMDTYISLLEGNAIPEDCYINNAIEITKLLINLYSECEKIYL"
     gene            1913..>2542
                     /locus_tag="ACGEUI_001731"
     CDS             1913..>2542
                     /locus_tag="ACGEUI_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFF8166534.1"
                     /translation="MDLKITNGFYDPSHLSYEVVERKGLGHPDTLADGIAEQIEIDYS
                     LYCLDKFGVIPHHNFDKIIIRGGHSVQDFGGSDFIEPIKIIFLGRASKKCFNSSIPLF
                     KIQKKAATKYLNRILPNLDVENYVEFETLTSDFTTKTNWFSPEAIEDLPEYLDVPKAN
                     DTATMISYWPLTISEELALMIEGYFYKLDKNELPTPRFTKMGGDIKVMVV"
BASE COUNT          950 a          308 c          493 g          791 t
ORIGIN      
        1 atttaggaat acattatgta gaactaggta aaggaattat cgattataaa acaatcattc
       61 atgaaatcca tgaacatttt atagacttaa tttttagttt agaagtacat gaaaaagaaa
      121 tagacaaaaa ggcagaagta gtgaggaagt cttatctgac actaaatgag tatattaatg
      181 gaggctaaaa tgaatttgag tatatttggt ttaaagaata ttccttattt gaaagaaggg
      241 gatagtatag aaaagcttat tgaagaaagc attaaaactt cagaattttt tatcgaagac
      301 aatgatgtgc tttgcatagc ttctaaagtt gtatctatcg cagaaggcca ggtgatgtct
      361 ttaaacgaga tacaagtgag tgatgtagca aaagaaatcc acaggaatat tcctcgtaaa
      421 gatcctagaa taattgaaat tatgctaaat ttagtcaata gagatctgtc acgtttagat
      481 atcaaaaaaa attacattgg ttgtcgcttg gaaaatggtt taaagttgac aagtggtgga
      541 atcgatagaa aatcagtaga tgaagtattt cttttaccta ataatccgga tgcttctgca
      601 aaaagaattt cggaatatct taaaaaatca cttggaaaaa atgttgcagt tgttataaca
      661 gatagtgatg gtagagagga taaaagaggt gctactcaag tagcaattgg tatttatggt
      721 attcatcctt tgagaaaaac agaagtaata gattcacaag gagagacaat aaaattccaa
      781 gaagagactt tgtgtgatat gattgcagct tgtgctggct tagtaatggg gcaacgtgga
      841 actggaattc cagctgtcct cattaggggc ttggattata aatggtctga aaatactagt
      901 attaaggaag cattaaatga gtagaaatca ggactgtaag aatattctaa tcattggttt
      961 tggaaaaatt gggaaaatta aagcgtacaa gtggctatca cgcggatatg aggtatatgt
     1021 caaagatttg aaattctcca gcatgaaata ccttaataca aatagtgatg tgttggatga
     1081 tttatcacga caatatttta ctattgagat ttgtactccg acaaatcacc accttacgct
     1141 attgaaaacc gttataacaa ggtatgtcta ttcttatatt agcattgaaa aaccactgtg
     1201 taataggtta gaagatttag aagaaatgaa gtcgctagta gaaagtcacc cacatctagc
     1261 aaaaaaaatt tttgccagtg aacaatactt cttttcgaaa attttggaaa aactgttaca
     1321 agtagattgt ttctctctag ataaaatcaa ggaaataaca gttgattttt ctaaagatag
     1381 gattgaggat aacgagaatg ggagattttt agataaagag atattgggct atggaataga
     1441 gattcctcat atcattgctg tcatttctta tttaggaatt tcattagaag aattcaaaaa
     1501 aggaatcttt gtgaatgcta tttatataaa agacttagag aaacacgatt attcaataga
     1561 aatcttatct tgtattaatc aaacagtgat taaaattatt agtaatttgg gaagttttgc
     1621 aataagagag ggcaagattg aaggaggtga tgctaaaaca gtaagaagag ttaccattga
     1681 tgacaaaaca atactatttg atccacatcc taaactcgag aggtataaga gtgaattggt
     1741 aactgggaca aatacgtttc aaatacacga tgatatgatg gacacgtata tcagtttgtt
     1801 agaaggaaat gctattcctg aagattgcta tatcaataat gcaattgaga taacaaaatt
     1861 attaattaac ttatattcag aatgtgaaaa aatttattta tgaggtgtaa ctatggattt
     1921 aaaaattaca aacggctttt atgacccaag tcatttatca tacgaagtag tagaacgcaa
     1981 aggcttaggt caccctgata ccttagcaga tggaattgct gagcaaattg aaattgatta
     2041 ctcactttat tgtttggaca agtttggtgt tattccacat cataattttg ataagattat
     2101 tatccgaggg ggacattcag ttcaggattt tggtggaagt gactttattg aaccaattaa
     2161 aattattttt ctaggtaggg cgagcaaaaa atgttttaat agttcaattc cactttttaa
     2221 aattcagaaa aaggctgcaa ctaaatattt aaatagaatt ttacctaatt tagatgttga
     2281 aaattatgta gaatttgaga cattgacctc agattttact acaaaaacta attggttctc
     2341 gcctgaagca atagaagatt taccggagta tctagatgta ccaaaggcaa atgatacagc
     2401 tacaatgata agttattggc cattaacaat ttctgaagaa ttagcattaa tgattgaagg
     2461 gtatttttat aaattagata agaatgaatt accaacgcca agatttacta aaatgggtgg
     2521 tgatattaaa gtaatggtcg ta
//
LOCUS       DAXWIQ010000087         2445 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000087 DAXWIQ010000000
VERSION     DAXWIQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.087"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(<1..298)
                     /locus_tag="ACGEUI_001732"
     CDS             complement(<1..298)
                     /locus_tag="ACGEUI_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HFF8166535.1"
                     /translation="MVQIFLSNLLKIKNTSISKLILIFPIICVMLSLLFSALGGENIL
                     RLTAETTVNQWGVIWINVIIAVNSGLLNKLELDSNKYSVFLSRDVDLKKVEYARV"
     gene            complement(304..993)
                     /locus_tag="ACGEUI_001733"
     CDS             complement(304..993)
                     /locus_tag="ACGEUI_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8166536.1"
                     /translation="MLKIQNLKKSYGKRTILNNVNMNIPKGKVYALIGPNGAGKSTIM
                     KILTGLVSKTSGSIIFEGREWSRRDLRKIGSIIEEPPLYKNLSAYDNMKVVTTMLGVS
                     ESTILPLLNKVGLGNIDKRPVKQFSLGMKQRLGIAISLINSPKLLILDEPTNGLDPIG
                     IQELREIIESFKSEGMTIMISSHILSEVEHLADFIGFIYEGKIILEKEYDGSENLEEL
                     FNNQILFEKRR"
     gene            complement(1102..1623)
                     /locus_tag="ACGEUI_001734"
                     /pseudo
     CDS             complement(1102..1623)
                     /locus_tag="ACGEUI_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1807..1947)
                     /locus_tag="ACGEUI_001735"
     CDS             complement(1807..1947)
                     /locus_tag="ACGEUI_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic streptin"
                     /protein_id="HFF8166537.1"
                     /translation="MNNTIKDFDLDLKTNKKDTATPYVGSRYLCTPGSCWKLVCFTTT
                     VK"
     gene            complement(2095..>2445)
                     /locus_tag="ACGEUI_001736"
     CDS             complement(2095..>2445)
                     /locus_tag="ACGEUI_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8166538.1"
                     /translation="TSFYGDEENLIRALSNLLVNAIRFMPVLDKKIEVILSESGEQIH
                     FEIWNNGERFSDSTLKKGDKLFYTEDYSRGNKHYGIGLAFVKGVAIKHGGNLQLNNPA
                     RGGASAIISIKKKI"
BASE COUNT          809 a          466 c          291 g          879 t
ORIGIN      
        1 gaactctggc atattctacc ttttttaaat caacatccct tgataaaaaa acagaatact
       61 tattagaatc taattctaat ttatttaaca aaccagaatt tacagcaata ataacattaa
      121 tccaaataac tccccactga tttactgttg tttccgcagt taaacgtagg atattttcac
      181 cacctaatgc tgaaaacaat aaagataaca tcacacaaat aatagggaaa ataagaatca
      241 acttgctaat actcgtattt tttattttta ataaatttga taaaaaaatt tgtaccatag
      301 cccctacctc ctcttttcaa ataaaatttg attattgaat aactcttcaa gattctcaga
      361 gccgtcatat tctttttcca gaataatctt tccttcatag ataaatccaa taaaatcagc
      421 tagatgttca acttctgaca gtatatggct tgaaatcata attgtcattc cttctgattt
      481 aaatgactct ataatttccc ttaattcttg aattccaatt gggtccaagc cattagtagg
      541 ttcgtctaat ataagtagtt taggtgaatt tattaaagag atagctatac ctaaccgttg
      601 cttcattcca agagaaaatt gttttactgg tctcttgtca atatttccta gaccaacttt
      661 atttaataat ggaagtatag tgctttctga aacaccaagc attgttgtaa ctaccttcat
      721 attatcgtaa gcactcaaat ttttataaag tggtggttct tcaataatac tcccgatttt
      781 tcgcagatcc cgacgtgacc attctctacc ttcaaaaata atagaaccac ttgtcttact
      841 aactaagcct gtcaaaattt tcataatagt tgattttcca gcaccatttg gaccgattaa
      901 agcatacact tttcccttag gaatattcat attgacatta ttcaatatgg ttctcttacc
      961 ataagatttt tttagatttt gtattttcaa catattagtt actcccattc tatatattgc
     1021 tgagcgttta gtgtatagta agcaaaacaa tacactttct cttcaaactc tctatcaatc
     1081 ccgtttaatc gattcatcga tagttgtaaa atgaaactta ccatcactat atcttatata
     1141 aatatcctcc aatcgcagtt cattctctga atcccatgat gtggtaaaaa atgaagtatt
     1201 ataagactca tcactatata actttgataa ttcacccaat ccaacatttt cacgcagtgt
     1261 caattcatac tttaaaaaat cttggaacaa tgcggatatt ctcttgtagt atgacttttt
     1321 ctgtattgaa caaagattaa taccatttat atatatattg ccttcataat tatcgtaaag
     1381 accgcaggct aatttgaaaa tcgtactttt tccagaacca tttccaccaa caattgctaa
     1441 cctctctcct gctttaattg aaaaacttat atcgtttatt acattatcct taccattata
     1501 tgaaaaagaa accctttcaa attcaactga ctgaattgta tcaaccgtac tttcgattat
     1561 agggttatgt actaactcat catgcgtaaa atcatgcaat aactccatgt acaatgaacc
     1621 atttagggag aaaccagcct cttttacgag atgaattgtt tttattagat tctttagctg
     1681 agctttaatc atgtataact tccttatcta ctaataaagg agcaaactaa aatcagtcta
     1741 ctcccctaaa tttataaata tataaaatta tttgaaatta taatgtttat gataatgata
     1801 actttattat ttaacagttg ttgtaaagca aactaatttc caacaagaac caggggtaca
     1861 taggtaacgg ctaccaacat aaggtgtagc agtgtctttt ttatttgttt tcaaatcgag
     1921 atcaaagtct ttaattgtgt tattcatttt ctagttcctc tcttacgttt ttgcatccga
     1981 tatatttaca tgattattat gacaccccaa gttcagaatt tgttcagaaa aaaagtattt
     2041 tagccaaaaa tttacgattt tttgcctgaa atactccagt ttcaatcaaa tctattaaat
     2101 tttctttttt atcgagatta tggcgctagc acctcctcta gctggattat tcaattgaag
     2161 attcccaccg tgtttgatag caacgccttt aacaaatgcc aaaccgattc cataatgttt
     2221 gttacctctg ctataatctt ccgtgtaaaa taatttatct ccctttttca gtgtactatc
     2281 actgaaacgc tcaccattat tccatatttc aaaatgaatt tgctctcctg actcagacag
     2341 tataacttcg atttttttat ctaatacggg cataaatctt attgcattaa ctaaaaggtt
     2401 agaaagagct cttatcagat tttcctcatc tccataaaaa gaagt
//
LOCUS       DAXWIQ010000088         2354 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000088 DAXWIQ010000000
VERSION     DAXWIQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.088"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..151
                     /locus_tag="ACGEUI_001737"
     CDS             <1..151
                     /locus_tag="ACGEUI_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFF8166539.1"
                     /translation="NELSKSTKKEKQLAERLKDDNFVKKYARAKYYLSREGEMIYPIP
                     GLLPK"
     gene            148..273
                     /locus_tag="ACGEUI_001738"
     CDS             148..273
                     /locus_tag="ACGEUI_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0009 family protein"
                     /protein_id="HFF8166540.1"
                     /translation="MMMDNIVKKIEAFLAFSDKKLAELQQENQKVKEESQYIQKN"
     gene            286..1572
                     /locus_tag="ACGEUI_001739"
     CDS             286..1572
                     /locus_tag="ACGEUI_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFF8166541.1"
                     /translation="MRKLLAAMLMTFFLTPLPVISTEKKLIFSKNAVYQLKQDVVQST
                     QFYNQIPSNPNLYQETCAYKDSDLTLPAGRLGVNQPLLIKSLVLNKESLPVFELADGT
                     YVEANRQLIYDDIVLNQVDIDSYFWTQKKLRLYSAPYVLGTQTIPSSFLFAQKVHATQ
                     MAQTNHGTYYLIDDKGWASQEDLVQFDNRMLKVQEMLLQKYNNPNYSIFVKQLNTQTS
                     AGINADKKMYAASISKLAPLYIVQKQLQKKKLAENKTLTYTKDVNHFYGDYDPLGSGK
                     ISKIADNKDYRVEDLLKAVAQQSDNVATNILGYYLCHQYDKAFRSEIKALSGIDWDME
                     QRLLTSRSAANMMEAIYHQKGQIISYLSNTEFDQQRITKNITVPVAHKIGDAYDYKHD
                     VAIVYGNTPFILSIFTNKSTYEDITAIADDVYGILK"
     gene            1569..>2354
                     /gene="tilS"
                     /locus_tag="ACGEUI_001740"
     CDS             1569..>2354
                     /gene="tilS"
                     /locus_tag="ACGEUI_001740"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFF8166542.1"
                     /translation="MMTYQEIFNEIKNKAYFKNHRHVLIAVSGGVDSMNLLHFLYLFQ
                     DKLKIRIGIAHVNHKQRSESDSEEAYLKCWAKKHDIPIYVSNFEGIFSEKAARDWRYA
                     FFKSIMLKNNYSALVTAHHSDDQAETILMRLIRGSRLRHLSGIKSVQPFANGQLIRPF
                     LTFSKKDLPEIFHFEDSSNRELSFLRNRVRNNYLPLLKQENPRFIQGLNQLALENSLL
                     FQAFKELTNHITTTDLTEFNEQSKSIQYFLLQDYLEGFPDLDLK"
BASE COUNT          881 a          349 c          371 g          753 t
ORIGIN      
        1 taatgaattg tcaaagagta caaaaaaaga aaaacaatta gcagaacgac taaaagatga
       61 taattttgtc aaaaaatatg ctagggcaaa atactattta tcgcgtgaag gagaaatgat
      121 ttatcctatt ccaggactat taccaaaatg atgatggaca atattgtaaa aaaaatagaa
      181 gcatttcttg ctttttctga taaaaaatta gcagagctgc aacaggaaaa tcaaaaagtt
      241 aaagaagaaa gtcagtatat tcaaaaaaac tagaaaggag tcaatatgag aaaattatta
      301 gcggctatgt taatgacttt ttttctgact cctttaccag tgattagtac agaaaaaaaa
      361 cttatatttt caaaaaatgc tgtttatcaa ttgaaacaag atgtcgttca atcaacacaa
      421 ttctataatc aaataccctc taatccaaat ctttatcaag aaacgtgtgc ctataaagac
      481 agtgatttaa ctctaccagc aggaagatta ggtgtaaatc aaccattact tattaaatcg
      541 cttgtgctta acaaagaatc tttaccggtt tttgagttag ctgatggtac ctatgttgag
      601 gctaatcgac aattgattta tgacgatatt gtacttaatc aagtagatat agatagctat
      661 ttttggacac aaaagaaact taggctttat tcagcccctt atgttttagg tacgcaaaca
      721 attccttctt cttttttatt tgctcaaaaa gttcatgcca ctcaaatggc acaaacaaac
      781 catggaactt attatcttat tgatgataag ggctgggcat cacaagaaga tctagttcaa
      841 tttgataacc gcatgttaaa agtccaggaa atgctcttac aaaaatataa taacccaaat
      901 tattcaattt ttgtaaagca actcaacaca caaacaagtg ctggtattaa tgctgataaa
      961 aaaatgtatg ctgcaagtat ctcgaagtta gcaccacttt atattgttca aaaacaatta
     1021 caaaaaaaga aattagcaga gaataaaact ttgacttata ctaaagatgt taatcatttt
     1081 tatggagact atgatccatt gggaagtggt aaaattagta aaatagctga taataaagat
     1141 tatcgtgttg aagacctact gaaagctgta gcacaacaat cggataatgt agcaactaat
     1201 attttaggtt attatctatg tcatcagtat gataaagctt tccgctcaga gataaaagct
     1261 ttatcaggta tcgattggga tatggagcag cgcttattaa cttctcgttc agctgcaaat
     1321 atgatggaag ctatttatca tcaaaaaggc caaattattt cttacctttc aaataccgaa
     1381 tttgatcaac aacgtatcac aaaaaatatt actgttccag ttgcacataa aattggtgat
     1441 gcttatgatt ataaacatga cgttgctatt gtttacggta atactccatt tattttgtct
     1501 atttttacaa ataaatcaac ctatgaagat attacggcta ttgcagatga cgtttatggt
     1561 attttaaaat gatgacctat caagaaattt ttaatgaaat aaaaaataaa gcctacttta
     1621 aaaatcatag acatgtgtta atagccgtat ctggtggagt agattcaatg aatttattgc
     1681 actttttata cctgtttcaa gacaaattga aaattcgtat tggtattgct catgttaatc
     1741 acaaacaaag atcagaatca gattcagaag aagcttattt aaagtgttgg gctaaaaaac
     1801 atgacatacc tatttatgtt tctaattttg aaggaatatt ttcagaaaaa gcagcacgtg
     1861 attggcgcta tgcttttttt aagagtatta tgttaaaaaa taactattca gctttagtaa
     1921 cagctcacca ttcagatgat caagcagaaa ctattttgat gaggctaatt agaggaagcc
     1981 gattgaggca cttgtcgggt ataaaaagcg tgcaaccatt tgccaatggt caacttataa
     2041 gacctttttt aactttctca aaaaaggact tgccagaaat ttttcatttt gaagattctt
     2101 caaatagaga attatcattt ttaagaaata gagttagaaa taattattta ccactgttaa
     2161 agcaggaaaa tcctaggttc attcagggat taaatcaatt agcacttgaa aatagcttac
     2221 tttttcaagc ttttaaggag ctaacaaatc atattactac cactgattta acagaattta
     2281 atgagcaatc taagtcaatt caatactttt tgttacaaga ttatttagaa ggttttccag
     2341 atttagattt aaaa
//
LOCUS       DAXWIQ010000089         2303 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000089 DAXWIQ010000000
VERSION     DAXWIQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.089"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..1138
                     /gene="hasA"
                     /locus_tag="ACGEUI_001741"
     CDS             <1..1138
                     /gene="hasA"
                     /locus_tag="ACGEUI_001741"
                     /EC_number="2.4.1.212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hyaluronan synthase HasA"
                     /protein_id="HFF8166543.1"
                     /translation="TYLVIKLGLSFLYEPFKGKPHDYKVAAVIPSYNEDAESLLETLK
                     SVLAQTYPLSEIYIVDDGSSNTDAIQLIEEYVNREVDICRNVIVHRSLVNKGKRHAQA
                     WAFERSDADVFLTVDSDTYIYPNALEELLKSFNDETVYAATGHLNARNRQTNLLTRLT
                     DIRYDNAFGVERAAQSLTGNILVCSGPLSIYRREVIIPNLERYKNQTFLGLPVSIGDD
                     RCLTNYAIDLGRTVYQSTARCDTDVPFQLKSYLKQQNRWNKSFFRESIISVKKILSNP
                     IVALWTIFEVVMFMMLIVAIGNLLFNQAIQLDLIKLFAFLSIIFIVALCRNVHYMIKH
                     PASFLLSPLYGILHLFVLQPLKLYSLCTIKNTEWGTRKKVTIFK"
     gene            1174..>2303
                     /gene="hasB"
                     /locus_tag="ACGEUI_001742"
     CDS             1174..>2303
                     /gene="hasB"
                     /locus_tag="ACGEUI_001742"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase HasB"
                     /protein_id="HFF8166544.1"
                     /translation="MKIAVAGSGYVGLSLGVLLSLQNEVTIVDILPSKVDKINNGLSP
                     IQDEYIEYYLKSKQLSIKATLDSKAAYKEAELVIIATPTNYNSRINYFDTQHVETVIK
                     EVLSVNSHATLIIKSTIPIGFITEMRQKFQTDRIIFSPEFLRESKALYDNLYPSRIIV
                     SCEENDSPKVKADAEKFALLLKSAAKKNNVPVLIMGASEAEAVKLFANTYLALRVAYF
                     NELDTYAESRKLNSHMIIQGISYDDRIGMHYNNPSFGYGGYCLPKDTKQLLANYNNIP
                     QTLIEAIVSSNNVRKSYIAKQIINVLEERESPVKVVGVYRLIMKSNSDNFRESAIKDV
                     IDILKSKDIKIIIYEPMLNKLESEDQSVLVNDLENFKKQANI"
BASE COUNT          793 a          366 c          386 g          758 t
ORIGIN      
        1 aacctatcta gttattaaac ttggattatc tttcctttat gagccattta aaggaaagcc
       61 acatgactat aaagttgctg ctgtaattcc ttcttataat gaagatgccg agtcattatt
      121 agaaactctt aaaagtgtgt tagcacagac ctatccgtta tcagaaattt atattgttga
      181 tgatgggagt tcaaacacag atgcaataca attaattgaa gagtatgtaa atagagaagt
      241 ggatatttgt cgaaacgtta tcgttcaccg ttcccttgtc aataaaggaa aacgccatgc
      301 tcaagcgtgg gcatttgaaa gatctgacgc tgacgttttt ttaaccgtag attcagatac
      361 ttatatctat ccaaatgcct tagaagaact cctaaaaagc ttcaatgatg agacagttta
      421 tgctgcaaca ggacatttga atgctagaaa cagacaaact aatctattaa cgcgacttac
      481 agatatccgt tacgataatg cctttggggt ggagcgtgct gctcaatcat taacaggtaa
      541 tattttagtt tgctcaggac cattgagtat ttatcgacgt gaagtgatta ttcctaactt
      601 agagcgctat aaaaatcaaa cattcctagg tttacctgtt agcattgggg atgatcgatg
      661 tttaacaaat tatgctattg atttaggacg cactgtctac caatcaacag ctagatgtga
      721 tactgatgta cctttccaat taaaaagtta tttaaagcaa caaaatcgat ggaataaatc
      781 tttttttaga gaatctatta tttctgttaa aaaaattctt tctaatccca tcgttgcctt
      841 atggactatt ttcgaagtcg ttatgtttat gatgttgatt gtcgcaattg ggaatctttt
      901 gtttaatcaa gctattcaat tagaccttat taaacttttt gcctttttat ccatcatctt
      961 tatcgttgct ttatgtcgta atgttcatta tatgatcaaa catcctgcta gttttttgtt
     1021 atctcctctg tatggaatat tacacttgtt tgtcttacag cccctaaaac tttattcttt
     1081 atgcaccatt aaaaatacgg aatggggaac acgtaaaaag gtcactattt ttaaataata
     1141 tgtgcatcga gtagttagag aaggagtaat tttatgaaaa tagcagttgc tggatcagga
     1201 tatgttggat tatcactagg agttctttta tcacttcaaa acgaagtcac tattgttgat
     1261 attcttccct ctaaagttga taagattaat aatggcttat caccaattca agatgaatat
     1321 attgaatatt acttaaaaag taagcaatta tctattaaag caactttaga tagcaaagca
     1381 gcttataaag aagcggaact ggtcattatt gccacaccta caaattacaa cagtagaatt
     1441 aattattttg atacacagca tgttgaaaca gttatcaaag aggtactaag cgttaatagc
     1501 catgcaactc ttatcatcaa atcaacaatt ccaataggtt tcattactga aatgagacag
     1561 aaattccaaa ctgatcgtat tatcttcagc cctgaatttt taagagaatc taaagcttta
     1621 tatgacaact tatatccaag ccgaattatt gtttcttgtg aagaaaacga ttctccaaaa
     1681 gtaaaggcag acgcagaaaa atttgcactt ttattaaagt ctgcagctaa aaaaaataat
     1741 gtaccagtac ttattatggg agcttcagaa gctgaagcag taaaactatt tgccaatact
     1801 tatttagcgt taagggtagc ttattttaat gagttagaca cttacgcaga atcgagaaaa
     1861 ttaaatagtc acatgattat tcaaggaatt tcttatgatg atcgaatagg aatgcattat
     1921 aataacccat catttggtta tggaggttat tgtctaccta aagatacgaa gcaattattg
     1981 gcaaattaca ataatattcc tcaaacgcta attgaagcta tcgtttcatc aaataatgtg
     2041 cgcaagtcct atattgctaa gcaaattatc aacgtcttag aagagcggga gtccccagta
     2101 aaagtagtcg gggtttaccg tttaattatg aaaagtaact cagataattt tagagaaagt
     2161 gctatcaaag atgttattga cattcttaaa agtaaagaca ttaagataat tatttatgag
     2221 ccaatgttaa acaaacttga atctgaagat caatctgtac ttgtaaatga tttagagaat
     2281 ttcaagaaac aagcaaatat tat
//
LOCUS       DAXWIQ010000090         2127 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000090 DAXWIQ010000000
VERSION     DAXWIQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.090"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            127..2118
                     /gene="uvrB"
                     /locus_tag="ACGEUI_001743"
     CDS             127..2118
                     /gene="uvrB"
                     /locus_tag="ACGEUI_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFF8166545.1"
                     /translation="MIDKRDDKPFKLKSKYKPSGDQPQAIESLVDNIEGGEKAQILLG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSAVSLRPGQEISRDTLLNQLVDIQFERNDIDFQRGCFRVRGDVVEVFPASRDEHAFR
                     VEFFGDEIDRICEIESLTGKTIGEVDHLVLFPATHFVTNDEHMEQSIAKIQAELAEQL
                     QLFESEGKLLEAQRLRQRTEYDIEMLREMGYTSGVENYSRHMDGRSPGEPPYTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDQARKQMLVDYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGEYEMSQTNTIIEQIIRPTGLLDPEIDVRSSMGQMDDLLGEINQRVARD
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNVDGHVIMYADKMTDSMQ
                     RAIDETARRREIQIAYNKAHGIVPQTIKKDIRGLISISKTSHNDISKEEMDYESMSRG
                     ERKEAINALQKQMQEAAELLDFELAAQMRDLILELKLMD"
BASE COUNT          707 a          356 c          462 g          602 t
ORIGIN      
        1 atttgttatt tatatgtcat aaaacataac atgtaaatga catgtttttt acgcttattg
       61 gaggtgattc tggcttatct atgttaaaat agtctataag agagtaagac tattagaggg
      121 tataaaatga ttgataaacg tgatgataaa ccatttaaac taaaatcaaa gtataaaccg
      181 tctggtgatc agccacaagc tattgaaagc ctagttgata atatcgaggg cggagaaaaa
      241 gctcagattc tcctaggtgc tacaggaacg ggaaaaacat acaccatgag ccaagtaatt
      301 agtaaagtaa ataaacctac cttagtgata gctcacaata aaaccttagc aggacaatta
      361 tatggtgaat ttaaagaatt ttttccagac aatgctgtgg aatattttgt ttcctattat
      421 gattactacc aacctgaagc atatgtccca tcaagcgaca cctatatcga aaaagatagt
      481 tcagtcaatg atgaaatcga taaacttcgc cattcagcca cctcttcttt acttgagcgt
      541 aacgatgtca tagtggtagc ttctgtatct tgtatttatg gactaggttc gccaaaagag
      601 tacgcagatt cagcagtcag cctacgtcca ggtcaggaga tatcacgtga cactttgcta
      661 aatcaattag tagatattca atttgagcgt aatgatattg attttcaacg cggctgtttt
      721 cgtgtacgtg gcgatgttgt tgaggtcttt ccagcatcca gggatgaaca tgcttttcgg
      781 gtggaatttt tcggggatga gattgaccgt atatgtgaaa tcgaaagttt aactggtaaa
      841 actattggag aagttgacca tttggtttta tttccagcta ctcactttgt aacaaatgat
      901 gagcatatgg agcaatcaat agccaaaatt caagcagagt tagcagagca gttacagtta
      961 tttgaatctg aaggtaaact actagaagca cagcgattac gccaaagaac ggaatatgat
     1021 attgaaatgt tacgtgaaat gggctatact agcggagtcg aaaactattc ccgtcatatg
     1081 gatggcaggt caccaggaga acctccttat accttgttag atttcttccc agaagatttt
     1141 ttaattatga ttgatgaaag tcacatgaca atgggacaaa tcaaaggcat gtataatggt
     1201 gaccaagcca gaaaacaaat gctagttgac tatggttttc gattgccatc agctcttgat
     1261 aatcgtccct taagacgcga ggaatttgaa agtcatgtgc accaaattgt ctatgtttca
     1321 gcaacgcctg gtgaatacga aatgtcacag actaatacaa ttattgagca aattattcgt
     1381 ccgactgggc tacttgatcc tgaaattgat gtaaggtcaa gtatggggca aatggatgat
     1441 ttgttgggag aaattaacca gcgcgtagcg cgcgatgaac ggacgttcat cacaacctta
     1501 actaaaaaaa tggcagaaga tttgactgat tatctaaaag agatgggtgt caaggtcaag
     1561 tatatgcaca gtgatattaa aaccttagag cgaacagaaa ttattcgtga tttacgactt
     1621 ggtgtttttg atgtcttgat tggaatcaat ctgctccgtg aagggattga tgtgccagaa
     1681 gttagtcttg tagccatttt ggatgctgat aaagagggtt tcctccgtaa tgaacgcggc
     1741 ttgattcaga ccatcggtag agcagctcga aacgtagatg gtcatgtcat tatgtatgcg
     1801 gacaagatga cagattctat gcagcgagct attgacgaaa cggctcgtcg tcgtgagatt
     1861 caaatagcat ataacaaagc gcacggcatc gttccacaaa ctataaaaaa agacatcaga
     1921 ggccttatta gtataagtaa aacaagccac aatgatatat caaaagaaga gatggattat
     1981 gaaagtatgt caagaggaga acgaaaagaa gcgatcaatg ctctgcaaaa acaaatgcaa
     2041 gaggcagctg aattacttga tttcgaattg gcagctcaaa tgcgagactt gattttagaa
     2101 cttaaattaa tggactaggt tttgaat
//
LOCUS       DAXWIQ010000091         2070 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000091 DAXWIQ010000000
VERSION     DAXWIQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.091"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..404
                     /locus_tag="ACGEUI_001744"
     CDS             <1..404
                     /locus_tag="ACGEUI_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFF8166546.1"
                     /translation="LTTTGSCIDFGEEGAVGRGNKTHGIISSFRPNTMEAPAGKNCTY
                     FVGKVWGFLSDTIAKEIYEAFNTPCQIIMQLNIGSKLYRPTHLFIQTEESVDQERVLE
                     IVNRHLNNGKQNTNLILSTQHFIPKTNVYDG"
     gene            388..1356
                     /locus_tag="ACGEUI_001745"
     CDS             388..1356
                     /locus_tag="ACGEUI_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFF8166547.1"
                     /translation="MSMMDKTVLYFGLVEISFDVPDFIFQKIKEQNNKIRYYSKSINI
                     LGSVKFINSNQQFIEYDGNGNILVYGRLKYFKEGISLYYIGEYLAECLLIEHSDTLLI
                     HAAAVYNPLSGHSILLLGDKGAGKTTVAIRLCIEHGYHLIGNDQVIFGSNSGILLTYA
                     GTSFFKIRRTAVLSDNLLFKLFSKFFNRSLQFNKASWDDKISIFPKELGIRTCNFSTE
                     ISKIYYIKTDKKEKQIYHSIWSDSLASLYLNELLGRHISGQVACFQSDNGKYFSTMPL
                     INYEKNNRVREQIVKGIFAKQKLFKITAATPAKIAEAIIENVKKNS"
     gene            1411..>2070
                     /locus_tag="ACGEUI_001746"
     CDS             1411..>2070
                     /locus_tag="ACGEUI_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8166548.1"
                     /translation="MFVIGIPTLNEADNISRLVKQIDEYAVNLGKEIIIINSDSKSTD
                     GTPQIFLETKTYNTKVSIVSEAKGKGYNVRNIFEYAINHVPNFSGLILIDGDVVSMKK
                     MWLEKMFIAIESGNDLIIPNYARKSFEGNATNHFIYPMLVKIFKRDMPYQCISGDFGF
                     SRGLIKDLTLKCNWHKYTLGYGIDIFLTLTAILKSYKIKEIDLQSKIHKKSFEKIEKI
                     FL"
BASE COUNT          773 a          241 c          368 g          688 t
ORIGIN      
        1 atttaactac tacaggttct tgtattgatt ttggagaaga aggtgcagtt ggtcgaggta
       61 ataaaactca tggtatcata tcaagtttta ggccaaatac tatggaagca ccagctggaa
      121 aaaattgtac ttattttgta ggaaaggttt ggggattttt gtctgatact attgccaaag
      181 aaatttatga agcattcaat actccctgtc aaatcattat gcaattaaat attggatcaa
      241 aattgtatag accaacccat ttatttattc agacagaaga aagtgttgat caagaaagag
      301 tattagaaat tgttaacagg catttaaata atggtaaaca aaatactaat ttaatcttgt
      361 caactcaaca ttttattcct aaaaccaatg tctatgatgg ataaaactgt tttatatttt
      421 gggttggttg aaatttcttt tgatgtgcct gactttatat ttcaaaaaat aaaagagcag
      481 aataataaaa ttagatatta tagcaaatca ataaatatat taggtagtgt aaaatttatt
      541 aacagcaatc agcaatttat agaatatgat ggcaatggaa atattttagt ttatggaaga
      601 ttaaagtatt ttaaagaagg aatttcatta tactacattg gagagtactt agctgaatgt
      661 ctcttgattg aacatagtga caccttatta attcatgcag cagcagttta taatccttta
      721 tctggccatt caattttact attaggagat aaaggtgctg gaaaaactac ggttgcaata
      781 aggctttgta tagaacatgg gtatcacctt attggtaatg accaggtaat atttggttcg
      841 aactctggaa tactactaac ttatgcaggg acatctttct ttaaaataag aaggacagct
      901 gttttgtcgg acaatttact gtttaaatta tttagcaaat tttttaatcg ttcattacaa
      961 tttaataagg caagttggga tgataaaatt tcaattttcc caaaggagtt gggaatacga
     1021 acttgtaatt tttctactga aatctcaaaa atttattata taaagacaga caagaaagaa
     1081 aagcaaattt atcattcaat ttggagtgat agtttagcaa gtctatatct aaatgagcta
     1141 ttgggaaggc atatttctgg acaggttgca tgttttcaat cagataatgg aaaatatttt
     1201 tctacaatgc ctttgataaa ttatgaaaaa aataatcggg tacgagaaca gatagtgaag
     1261 ggtattttcg ctaaacaaaa attatttaaa attacagcag ctacgccagc taagattgca
     1321 gaagcgatta tagaaaatgt taagaaaaac tcatagtgtg ggtgaatttg gggattaata
     1381 tgaagtattg aaaaaggagt aagagtaatt atgtttgtga taggcattcc aactttaaat
     1441 gaggcagata atatcagtag attagttaaa caaattgatg agtatgctgt aaatttaggg
     1501 aaagaaatta ttattattaa ttcggatagt aaaagtacag atggaactcc gcagattttt
     1561 ttagaaacca aaacttataa caccaaagtt tcgatagtat cagaagctaa aggaaaaggt
     1621 tataatgtga gaaatatttt cgaatatgca ataaatcatg tgccgaattt ctccggttta
     1681 attttgattg atggagacgt tgtctcaatg aaaaaaatgt ggttagaaaa gatgtttata
     1741 gcaattgaat ctggaaatga tcttataatc ccaaattatg ctagaaaatc ttttgaagga
     1801 aatgcgacaa accattttat ttatcccatg ttagtgaaaa ttttcaaacg tgatatgcct
     1861 tatcaatgca tctctggtga ttttggattt tcaagaggat tgataaaaga tttaactttg
     1921 aaatgtaatt ggcacaaata tacattaggt tacgggatag atattttttt aaccttaaca
     1981 gctatattaa agtcatacaa aattaaggaa atcgatttac aatcaaagat tcataaaaaa
     2041 agctttgaga aaattgaaaa aattttttta
//
LOCUS       DAXWIQ010000092         2031 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000092 DAXWIQ010000000
VERSION     DAXWIQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.092"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            128..1420
                     /gene="purB"
                     /locus_tag="ACGEUI_001747"
     CDS             128..1420
                     /gene="purB"
                     /locus_tag="ACGEUI_001747"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFF8166549.1"
                     /translation="MLERYSRPEMAAIWTEENKYHAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEEIDELFNPIYYTKRVDDIFKRLGI"
     gene            1552..>2031
                     /gene="rgg4"
                     /locus_tag="ACGEUI_001748"
     CDS             1552..>2031
                     /gene="rgg4"
                     /locus_tag="ACGEUI_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Rgg4/ComR"
                     /protein_id="HFF8166550.1"
                     /translation="MLEHFGGKVKVLRLEKRISREDLCGDESELSVRQLARIELGQSI
                     PSLSKVIFIAKALNVSVGYLTDGADLELPKRYKELKYLILRTPTYMDDGKLQVREEQF
                     DEIFEDYYDKLPEEEKIIIDCLQATLDTLLSENTNFGIDLLQEYFNQIKTKVRFRQND
                     "
BASE COUNT          652 a          344 c          463 g          572 t
ORIGIN      
        1 tggaagaatt gataagagcg aaggaagagt tatacccaga agcttatcca gctaatatat
       61 ttaaaaatgg taaacgtact aaagaattaa aagaaaaaat gtctttttaa taagataagg
      121 ggaaaaaatg ctagaacgtt attcacgccc tgagatggcg gcaatttgga cagaggaaaa
      181 taaataccat gcttggttgg aggtcgagat tttggctgac gaggcatggg ctgagttggg
      241 tgagattcct aaggaggatg tggctaagat tcgtgagaag gcggattttg acattgaccg
      301 cattcttgaa attgagcagg acacgcgtca cgatgtggtg gctttcacgc gtgcggtttc
      361 tgaaacgctt ggtgaggagc gcaagtgggt gcactacggt ttgacctcga ctgacgtggt
      421 ggacactgcc tatggttacc tctacaagca agctaacgac attatccgtc gcgatcttga
      481 gaatttcacc aatatcgtgg cagacaaggc gcgtgagcac aaaatgacca tcatgatggg
      541 tcgtacccac ggtgttcacg ccgagccaac gactttcggt cttaagttgg cgacttggta
      601 cagcgagatg aaacgtaata ttgagcgttt tgaacatgct gccgcaggtg tggaagctgg
      661 taagatttca ggtgccgttg gtaactttgc caacatccca ccttttgtgg aagaatatgt
      721 ctgtgacaaa ttaggcattc gtccgcaaga aatttcaaca caagttcttc cacgtgacct
      781 tcacgcagaa tattttgcag tgcttgcaag tattgcaact tctatcgaac gtatggcgac
      841 agagattcga ggtctgcaaa agtcagaaca acgtgaagtt gaagaattct ttgccaaagg
      901 tcagaaaggt agctctgcta tgcctcacaa acgcaaccca atcggttcag agaacatgac
      961 agggctagcg cgcgtgattc gtggtcacat ggtgacagct tatgagaacg tgtcactttg
     1021 gcatgagcgt gacatttcgc actcatcagc tgagcgtatc atcacacctg acacaactat
     1081 cttgattgac tacatgctca accgctttgg taatatcgtt aagaacttga ctgtcttccc
     1141 ggaaaatatg atgcgcaata tggaatcaac ttttggtttg atttatagtc aacgtgttat
     1201 gctcaaattg attgaaaaag gaatgacacg agaagaagct tatgacttag ttcaacctaa
     1261 gacagcttat tcctgggaca atcaagtgga tttcaaacca cttttagaag aagacaccaa
     1321 agttacctct tgtcttacac aagaagaaat tgatgaacta tttaatccga tttattacac
     1381 aaaacgtgtt gatgatattt ttaagcgttt agggatttaa tataaaaaaa agaaggtgat
     1441 tcccttcttt tttatttaaa aaaacgaata ttttctttaa aaatattaaa aaggttagtt
     1501 tttaattaga cgctgtgata taataaagaa aacagttgtt ggagagaaac catgttagaa
     1561 cattttggtg gaaaagtaaa agtgttaaga cttgaaaaga ggattagtcg cgaggacttg
     1621 tgtggggatg agtctgaact ttctgttcgt caattagcac ggatagaact aggtcaatcc
     1681 ataccaagtt taagtaaggt tatttttatt gcaaaagcct taaacgttag tgtcggttac
     1741 ttaactgatg gtgctgattt agaactacct aagcgttaca aagaattaaa ataccttatc
     1801 ttaaggacac caacttacat ggatgatgga aaattacaag tacgagaaga gcagtttgat
     1861 gaaatttttg aggattatta tgataaatta ccagaggaag agaaaataat cattgattgt
     1921 ttacaggcaa ctttagatac tttattgagt gagaatacta actttggcat tgacttactt
     1981 caagaatatt ttaatcaaat aaagactaag gtacgcttta ggcaaaatga t
//
LOCUS       DAXWIQ010000093         1794 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000093 DAXWIQ010000000
VERSION     DAXWIQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.093"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(<1..1481)
                     /locus_tag="ACGEUI_001749"
     CDS             complement(<1..1481)
                     /locus_tag="ACGEUI_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HFF8166551.1"
                     /translation="MIELNDEFIRKETIELANDGPRVHTTQYETKVPRLHKCYLLFFS
                     IIISSLTIAVPFLTDAANGLQSQNLYIGMMLTKGQLPYSAAFTTGGLFYFVIIALSYY
                     LGSTLWLVFVQVFCFYLSGLYLYKLINYMTGFQKVALTFSISYYLLSVSLGFGGLYPT
                     QLAMPFILISAWFLTKYFACLVKDEAFILFGFVGALAMLIDPSTLIFWSFACVTVFSY
                     NISQKHLARGFYQLLASIFGMILVFYTAGYFILNLQVLNPYLSQTMIYPFTFFKSGNL
                     SLLFGLAIQLFFALGLGLLTGMENVIRRFKNNSDRVVKWLFVMVILESILVAIFSQDY
                     RPYHLLPLLPFGLILTAIPVGYQYGIGLGQSSHRRRHGKNGVGRVMMIYLKRHFYLPI
                     LIVGTILICSTYCFISSIPLNQERDHIASYLEQKLNKTQSIYVWDDTSKIYLDSKAKS
                     VSQFSSPDINTQKESHRKILEDELLENKAAYIVVNRYKNLPKI"
BASE COUNT          686 a          306 c          273 g          529 t
ORIGIN      
        1 atgattttag gcaggttttt atagcgatta acaacgatat aagcagcctt attttctaat
       61 agttcatctt ctaatatttt tcgatgactc tctttttgcg tattgatgtc aggagaacta
      121 aattgagaaa cagatttagc tttactgtcc aaataaattt tagaagtatc atcccaaaca
      181 taaatagatt gagttttatt tagtttctgt tctaaataac tagcaatatg atcacgctcc
      241 tgattaagag gaatactact aatgaaacaa taagtagaac agattagtat tgtccctaca
      301 attaaaattg gcaaataaaa gtgtctctta agataaatca tcattactcg accaacacca
      361 tttttaccat gacgtctgcg atgactactc tgacctaatc ctataccata ctgatagcca
      421 acaggaatag cagtcaaaat taatccaaaa ggtaaaagag gtaaaagatg ataggggcga
      481 tagtcttgtg aaaatatagc cacaagtata gattctagaa tgaccatgac aaatagccac
      541 ttgacgaccc tatcagaatt gtttttaaat cgcctaatga cattctccat tcccgtcaaa
      601 agaccaagcc ccaaagcgaa gaacaactga atagccagtc caaaaagcaa cgataagttt
      661 cctgatttaa aaaaagtaaa aggataaatc atcgtttgtg ataaataagg atttagcact
      721 tgtaagttca aaatgaaata tcctgctgtg taaaaaacta aaatcattcc aaaaatcgaa
      781 gctagcagtt gataaaaacc tcttgcaaga tgcttttggc ttatattata agaaaaaact
      841 gtcacacaag caaaagacca aaagataagg gtactcgggt caattagcat tgcaagagca
      901 cctacaaagc caaaaagaat aaatgcctca tctttcacta aacaggcaaa atacttagtt
      961 aaaaaccaag ccgatattaa tataaatggc atagccagtt gagtaggata caatccccca
     1021 aaaccaagac taacagataa taaatagtag ctaattgaaa aagttaaagc cactttttga
     1081 aagcctgtca tataattaat gagtttataa agatataaac cagataagta aaaacaaaac
     1141 acctgaacaa agactagcca aagtgtcgat cccaaataat aacttaatgc aatgataaca
     1201 aagtaaaaga gccctcctgt tgtaaaagca gcactatagg gaagttgccc cttcgttaac
     1261 atcattccta tatataagtt ttgggactga agaccattgg ctgcatctgt taaaaaaggt
     1321 actgcaattg ttaaagaact gataataatg ctaaagaaaa gcagatagca tttatgaagc
     1381 cgaggtactt tagtttcata ttgagtggtg tgtactcgtg ggccatcgtt ggccagttct
     1441 atcgtctctt ttcggatgaa ttcatcgttt aactcgatca ctatttttct ccttgaaata
     1501 tcatattctc taacgattca ataaaagcaa tatttcttca tatcactagt ctaacatagc
     1561 tattatccat tattttttag tatgagataa ataacatgac tttgtattgc acatagcaaa
     1621 gtgacagtac ataaacaaag taagttgaaa aatgatatta tcgtcaaaga atattataaa
     1681 acaagtataa cagaatcttt tatttagtca atttctgata tattttcaca tactgtaata
     1741 attctctaaa tatatgcaaa ggtttagcct tataattatt aataaattta tcat
//
LOCUS       DAXWIQ010000094         1784 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000094 DAXWIQ010000000
VERSION     DAXWIQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.094"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..281
                     /locus_tag="ACGEUI_001750"
     CDS             <1..281
                     /locus_tag="ACGEUI_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HFF8166552.1"
                     /translation="QEELNTDTNKFSGIVFFRLNPENYGKIFNGVSYDAPKTSLIFEE
                     KIYKKEGTVLPNPINWSNERVGYVCLENKEYAVLEKEMKEILKSLSIE"
     gene            311..1225
                     /locus_tag="ACGEUI_001751"
     CDS             311..1225
                     /locus_tag="ACGEUI_001751"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFF8166553.1"
                     /translation="MCSPSYPGTFHYQKRYKRGVEFLQNKGFTIVEGNLTGKEGYRGE
                     ISGGVQDRAEELNQLIPRVDVIMTSIGGYNSNSVLKYIDYDLFKQKSPIFIGYSDTTA
                     LALALYKKTGCITYLSQSVISNFGEFEPFNELNYFYFDFMLQSKCETLMVQIPDVWTD
                     EWINWETYERTKKTNKNEWIIFNKGEFNGTLIGGNLDTIVGIIGTEYMPKITEDTILL
                     LEDVYTDLGRLYRNFTTLALHGIFDKIGGLIISKFETIGENSDVINDIINEFVGHRKI
                     PILLNFDCGHTHPSCLMPIGGKITLSLS"
     gene            1238..1438
                     /locus_tag="ACGEUI_001752"
     CDS             1238..1438
                     /locus_tag="ACGEUI_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166554.1"
                     /translation="MNNRESKLTGFYDVFEEEVEKINHIHNVFVEESEKFGFQQMKIA
                     MVEHRKKYLDATDVHYTKYKNI"
     gene            1558..>1784
                     /locus_tag="ACGEUI_001753"
     CDS             1558..>1784
                     /locus_tag="ACGEUI_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166555.1"
                     /translation="MLFRDRIPTYKGYRRQFNQILLGAWGIESAYVDAEIIVATWRGL
                     QRFKGIKVEFIQLSNKNIFDVLEKDLSKKLR"
BASE COUNT          676 a          217 c          352 g          539 t
ORIGIN      
        1 tacaagaaga gttaaataca gacactaata agttttcagg aattgtattt ttcagactca
       61 atccagagaa ttacggaaaa atatttaatg gagtgagtta cgatgcacct aaaacttcac
      121 taatatttga agaaaagata tataaaaagg aaggaactgt attgccaaat ccgattaatt
      181 ggagtaatga gagagtagga tatgtctgtt tggaaaacaa ggaatacgct gtgctagaaa
      241 aggagatgaa agaaatatta aaatcattgt caatagagta aatagtgaaa gaaacatcaa
      301 atctataggt atttgttcac cctcctatcc ggggacattt cattatcaga aacgatataa
      361 gagaggagta gaatttttac aaaataaagg atttactata gtagagggaa atttaacagg
      421 aaaagaaggt tatagagggg aaatttcagg aggagttcaa gacagagctg aagaattaaa
      481 tcaacttatt ccgcgtgtgg atgtcatcat gacttccata ggtggctaca attctaattc
      541 tgtattaaag tatatagact acgatctatt taaacagaaa tctcctatct ttattggata
      601 ttctgacaca acagctcttg ctttagcctt atataaaaag actggatgta taacttacct
      661 gtctcagagc gttatctcta attttggaga gtttgaaccg tttaatgaat taaattattt
      721 ctattttgac ttcatgcttc agagtaagtg cgaaacctta atggtacaaa taccagatgt
      781 ttggacggat gagtggataa attgggaaac ctatgaaaga accaaaaaaa caaataaaaa
      841 tgagtggatt atttttaaca aaggtgaatt taatggtact ttgatcggag gaaatctcga
      901 taccattgta ggaataatag gaactgagta tatgccaaaa ataacagagg ataccatact
      961 cttgttagaa gatgtatata cggatctagg aagactatat aggaatttca caacgcttgc
     1021 cttacatggt atttttgata aaataggagg acttattatt tcaaaatttg aaacaatagg
     1081 ggaaaatagc gatgttatta atgatatcat taatgaattt gtagggcata gaaagattcc
     1141 tattttattg aattttgatt gtggtcatac ccatcctagc tgtttaatgc ctattggtgg
     1201 taaaattacg ttgagtttaa gctaaaagag gtggtatatg aacaatagag aaagtaaatt
     1261 aacgggtttc tatgatgttt ttgaggaaga agtagaaaaa ataaatcata ttcataatgt
     1321 ttttgtagag gagtcagaaa aatttggctt tcaacagatg aagattgcta tggtagagca
     1381 tagaaaaaaa tacttagatg caaccgatgt tcattataca aaatacaaaa atatttgaag
     1441 ttcatagagt aaaagaagag tcagtatacg ccctccaatc agatttggca cttagtatgt
     1501 cgccgttgta tagctaactt accacaacaa atacctgtaa agtggattca agacagagtg
     1561 ttatttagag atagaattcc tacatataaa ggttatagaa gacaatttaa tcaaatatta
     1621 ttaggtgcat ggggaataga atcagcttat gtggatgcag aaataatagt tgcaacttgg
     1681 cgaggcttgc aacgatttaa aggaattaaa gtagaattta ttcaactgtc taacaaaaat
     1741 atatttgatg ttttggagaa agatttatct aaaaagttaa gatt
//
LOCUS       DAXWIQ010000095         1782 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000095 DAXWIQ010000000
VERSION     DAXWIQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.095"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            251..>1782
                     /gene="mga"
                     /locus_tag="ACGEUI_001754"
     CDS             251..>1782
                     /gene="mga"
                     /locus_tag="ACGEUI_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor transcriptional regulator Mga"
                     /protein_id="HFF8166556.1"
                     /translation="MYVSKLFTSQQWRELKLISYVTENADAIGVKDKELSKALNISML
                     TLQTCLTNMQFMKEVGGITYKNGYITIWYHQHCGLQEVYQKALRHSQSFKLLETLFFR
                     DFNSLEELAEELFVSLSTLKRLIKKTNAYLMHTFGITILTSPVQVSGDEHQIRLFYLK
                     YFSEAYKISEWPFGEILNLKNCERLLSLMIKEVDVRVNFTLFQHLKILSSVNLIRYYK
                     GHSAVYDNKKTSQRFSQLIQSSLEFQDLSRLFHLKFGLYLDETTIAEMFSNHVNDQLE
                     IGYAFDSIKQDSPTGCRKVTNWVHLLDELEIRLNLSVTNKYEVAVILHNTTVLKEEDI
                     TANYLFFDYKKSYLNFYKQEHPHLYKAFVAGVEKLMRSEKEPISTELTNQLIYAFFIT
                     WENSFLKVNQKDEKIRLLVIERSFNSVGNFLKKYVGEFFSITNFNELDALTIDLEEIE
                     KQYDVIVTDVMVGKSEELEIFFFHKMIPEAIIDKLNAFLNISFADSLPLDKPINPLDF
                     HR"
BASE COUNT          628 a          332 c          311 g          511 t
ORIGIN      
        1 aaaaagagca taaatggcat aaaaaatggg taaaaatcca atttttgggt cacgttttat
       61 aatatgtttt ataaataatg aacaaaaaga aataaatgcg tgatcgtgac tgttttttgg
      121 attttaatga aagaatttga aaggcatttt atggtatact ttttcttaat ataggtcgta
      181 ctgacttaac gaaatacctc acgaaataac aagaaaatca agaaaattaa aaagaagggt
      241 atacaaggta atgtatgtaa gtaagttgtt tacaagtcaa cagtggagag aactaaaatt
      301 aatctcatac gtaacagaaa acgctgacgc cattggcgtc aaagataaag aactcagcaa
      361 agctctcaac atctccatgt taacattgca aacatgtctc actaacatgc aatttatgaa
      421 ggaagtggga ggaattacct ataaaaatgg ttatattaca atctggtacc atcaacattg
      481 cggtttgcaa gaagtctatc agaaagctct gagacactct caatcattta aattgctgga
      541 gacccttttt ttcagagact tcaactcttt ggaagaatta gctgaagagc tctttgtgag
      601 cctttcaaca ctcaagcgtc tcatcaaaaa aaccaatgcc tatttgatgc atacctttgg
      661 tattactatt ttaactagtc ccgtacaagt atcaggagat gaacaccaga ttcgactatt
      721 ttacctcaag tatttttcgg aagcctataa aatctcagag tggccttttg gggaaatcct
      781 aaacttaaag aactgtgagc ggctactcag tttaatgata aaagaggtcg atgtgagggt
      841 taattttaca ttatttcagc accttaaaat actaagctca gttaacttga ttcgttatta
      901 caagggacac tctgccgtct acgacaacaa aaaaaccagt caacgttttt ctcagctcat
      961 ccaatcatcc ttagaattcc aagacctttc acgcttgttt cacctcaaat ttggactata
     1021 cctagatgag actaccatag ctgagatgtt ctctaatcac gttaatgacc aattagaaat
     1081 cggttatgcg tttgatagca tcaaacaaga ctcaccaacg ggctgtcgaa aagtgaccaa
     1141 ctgggttcat ctccttgatg agttagaaat caggctcaat ctcagcgtca ccaacaaata
     1201 cgaagtagct gtcatccttc ataacactac cgtattgaaa gaagaagata tcacagctaa
     1261 ttacctattt ttcgattaca aaaaaagtta cctcaacttt tacaagcaag aacaccctca
     1321 tctttataaa gcatttgtag caggtgtaga aaaactgatg cgttcagaga aagaacctat
     1381 cagcacagag ttgactaacc aattgatcta cgcctttttc atcacttggg aaaatagttt
     1441 cctaaaagta aatcaaaaag atgaaaaaat tcgtcttctg gtgattgaaa gaagttttaa
     1501 cagtgttggt aatttcctaa aaaagtacgt cggagagttt tttagcatca caaacttcaa
     1561 tgagctagat gctctgacca tcgatctaga agagattgaa aaacagtatg atgtgatcgt
     1621 gacagatgtt atggtaggaa aaagcgaaga gctagaaatt ttctttttcc acaaaatgat
     1681 tccagaagcg attattgaca agctcaatgc gtttttaaac atcagctttg cagacagctt
     1741 gccactagac aaacccatca accccttgga ctttcatcgc aa
//
LOCUS       DAXWIQ010000096         1724 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000096 DAXWIQ010000000
VERSION     DAXWIQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.096"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            135..473
                     /locus_tag="ACGEUI_001755"
     CDS             135..473
                     /locus_tag="ACGEUI_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFF8166557.1"
                     /translation="MKPPFTVTNTMLNKVVEISKIIGNLELQVQKDLKLRKENRIQSI
                     HSSLAIEQNSLTVEQITAIIDGKRVLGNPREIREVKNAYEAYEEILTLTPYDESHFFK
                     MKKFQQYIYR"
     gene            497..1321
                     /locus_tag="ACGEUI_001756"
     CDS             497..1321
                     /locus_tag="ACGEUI_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase A"
                     /protein_id="HFF8166558.1"
                     /translation="MGLGSDSLPVKISLFDVDTYLADSMQFHLEYILRLFPKSGVHYL
                     MPTFRGEAVDHRHLSQFYHSECEIEGDLHYVMNLVDRYIKFLVSEILRECRDSIFAIT
                     KDVSHLESFIDRKIPIITFEEACTILENEIDSLEYNDEHHFYVLTDVGEKKLMEHFEG
                     VVWVKNYPCKSVPFYQRFSTDHLFAYNADLLLGIGETVGCGERCETYEEVVENMNYLQ
                     VDASEYSWYLEMKKTRPMKSSGFGLGVERLIAWILKCDDVRKVQLISRENGIDYLP"
     gene            1335..>1724
                     /locus_tag="ACGEUI_001757"
     CDS             1335..>1724
                     /locus_tag="ACGEUI_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HFF8166559.1"
                     /translation="MKKNILIVGGGPEITICYDLINNSTEYNFFSVSEIPTMYPELFV
                     ETIETSFRNFGKTIEQIKELKYSIDAVACWDEALTHIADDISKELGLNPISSLDSQSF
                     RFKDRMRMVCEAGGLKMPKYKIINQFSD"
BASE COUNT          631 a          198 c          339 g          556 t
ORIGIN      
        1 cgttaaaaag ttagtaatcg ttaaagaatt taaaataaat tgtaatagtt tatcgcttgt
       61 tttatcgctt gaagaacgag cgataaattt atttttatgg tataaataag aaaaaagatt
      121 gtaaggagaa aatgatgaaa ccacctttta cagttacgaa taccatgtta aataaggtag
      181 tagagatttc taaaattatt ggtaatttag aattacaagt acagaaagat ttaaaattaa
      241 gaaaagaaaa tcgtattcag tctattcact catcactcgc tattgaacaa aattctttaa
      301 cagtagaaca aataacagcc attattgatg ggaaaagagt gttagggaac ccccgtgaga
      361 taagagaagt aaaaaacgct tatgaagctt atgaagaaat tttaacgcta actccctatg
      421 atgaatcaca tttttttaaa atgaagaaat ttcaacagta catttaccga taacaacatc
      481 gtcaatcaca agtcctatgg ggttggggag tgattcgcta cctgtaaaaa tttccttatt
      541 tgatgttgat acctatcttg cagattcgat gcagtttcac ttagagtata tacttagact
      601 ttttccaaag tcaggagttc attacttaat gcctactttt agaggagaag cagtagatca
      661 tcgacattta tcgcaatttt atcatagtga gtgtgagata gagggagatt tacactatgt
      721 gatgaattta gtggatagat atattaagtt tctggttagt gaaattctta gagagtgtcg
      781 agatagtatt tttgctataa caaaagatgt aagtcatttg gaaagtttta ttgatcgaaa
      841 gataccaatt attacgtttg aagaggcttg tactatattg gaaaatgaaa tagattcatt
      901 ggaatataat gatgaacatc atttttatgt gttaacagat gttggggaaa agaaattaat
      961 ggaacatttt gagggagtag tttgggtaaa aaattatccc tgtaaaagtg tcccttttta
     1021 tcaaaggttc tcaacagatc atctatttgc ctataatgca gatttgttgt taggaatagg
     1081 cgaaactgta ggttgtggag aaagatgcga aacatatgaa gaagttgtag aaaatatgaa
     1141 ttatttacaa gtagatgctt ctgaatatag ttggtattta gaaatgaaaa aaacaagacc
     1201 tatgaaatct tcaggatttg gtttaggagt agagcgttta atagcgtgga tattgaagtg
     1261 cgatgatgtt cgaaaagttc aattaatcag tagagaaaat gggattgatt atttaccata
     1321 aaggaggaag aaaaatgaaa aagaatattt taattgtagg tggaggtcca gagataacta
     1381 tatgttatga tttaattaac aatagtacgg agtataattt cttttcagta tcagaaattc
     1441 ctacgatgta tccagagttg tttgtagaaa caattgaaac cagctttaga aattttggaa
     1501 agacgattga acaaattaaa gagctcaaat attctattga tgctgttgct tgttgggatg
     1561 aagctttaac gcatatagct gatgatattt caaaagaatt aggattgaac cctatttcat
     1621 cgctagattc tcaatcattc aggtttaaag atcgaatgag aatggtatgt gaagctggtg
     1681 gactaaaaat gcccaaatat aaaattatca atcaattttc agat
//
LOCUS       DAXWIQ010000097         1714 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000097 DAXWIQ010000000
VERSION     DAXWIQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.097"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            127..768
                     /locus_tag="ACGEUI_001758"
     CDS             127..768
                     /locus_tag="ACGEUI_001758"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFF8166560.1"
                     /translation="MKDLLKQYQAKPLGEEKRYAVFLPLILVNDDWHVLYEVRSQHIS
                     QPGEVSFPGGQVENQETLQEAAIRETVEELTVDASQIQLWGEIDYLVQSSRTIHCFVG
                     QLVVDDWKSIQPNEEVDKVFIVPLRQLLFTDPVYYRLEVTPIETTDFPFDRIRNGKYY
                     QFSQEYRSIPFYENLEETIWGMTAQFTKCLTDILNDESIAKKNPSDIEKSKGR"
     gene            complement(815..1657)
                     /locus_tag="ACGEUI_001759"
     CDS             complement(815..1657)
                     /locus_tag="ACGEUI_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFF8166561.1"
                     /translation="MVSFISRVFKGMIIALGFILPGVSGGVLAAILGIYERMISFLAH
                     MRDNFIENVLFFLPVGIGGILGIALFSFPVEFLLKHYQVSVLWGFAGAIVGTIPSLIK
                     ESTKQSQRDKADWLWLVLTFVISGLGLYFLNDLIGTLPANFLTFILAGALIALGVLVP
                     GLSPSNLLLILGLYGPMLIGFKSLDLLGTFLPIAIGGVLAILAFSKSMDYALQHHHSK
                     VYHFIIGIVLSSTLLILIPNSSSPESISYSHAGILTWLMAFVLFALGIWLGLWMSQLE
                     EKYK"
BASE COUNT          619 a          300 c          333 g          462 t
ORIGIN      
        1 atattttata tataaaaaaa taacatatat atcaaaaatc actaactagg agtaataagt
       61 tgaccaaaaa gctaggattg gttatgatga agataagatt aacggaaaga gaaattaggg
      121 aagactatga aggacttatt aaaacagtat caggcaaaac cactaggaga ggagaagcgg
      181 tacgccgtat ttttacctct tattcttgtc aatgatgact ggcatgtctt atatgaagtc
      241 agaagtcagc atatttcaca accgggggaa gtttctttcc cagggggaca agtagaaaat
      301 caggagacac ttcaagaagc tgctatccga gaaacagttg aggaattgac tgttgatgcg
      361 tcccagatcc agctatgggg tgaaattgat tatttagtac agtcttctag aactattcat
      421 tgtttcgttg gtcaattagt cgttgatgac tggaaaagca tacagcctaa cgaagaagtt
      481 gacaaggtct ttatagtacc tttacgtcag ctacttttca ctgatcctgt ctattatcgt
      541 ttagaagtca ctcctattga gacaacggat tttccttttg accgtattcg taatgggaaa
      601 tactaccaat ttagtcaaga gtaccgtagt attccgtttt atgaaaatct agaagaaacc
      661 atatggggaa tgactgccca gtttactaag tgtttaaccg atattctaaa tgatgaatca
      721 attgcaaaga aaaacccctc agatattgaa aaatctaagg ggagatagag cactagtttg
      781 ttggcgtcaa ctttttgtca acaaactgta aaggttattt gtatttttct tctaattgac
      841 tcatccaaag tccgagccaa ataccaagag caaataacac aaaggccata agccaggtta
      901 agatacctgc atgactgtag gaaatggatt cgggactact actatttgga ataagaatta
      961 atagcgtgct ggataataca ataccaatga taaagtggta aacttttgaa tgatggtgct
     1021 gtaaagcata atccatactt ttagaaaaag ccaagatggc taggactcct ccgatagcaa
     1081 taggtaagaa agtccctaat aaatccaaag atttgaaccc tattaacata ggaccataga
     1141 gtcctaagat aagtaagaga tttgacgggc ttagtccagg gaccagtact cctaaagcaa
     1201 ttaaggcacc tgctaaaata aaggttaaaa agttagctgg taaggtaccg attaagtcat
     1261 ttaagaagta aagacctagt cctgaaataa caaaggtaag gactagccat agccagtcag
     1321 ctttgtctct ttgagactgc ttggttgact ccttaatcag actaggaata gtgccgacaa
     1381 tagcgccagc aaatccccat aagacgctaa cttgatagtg cttaagcaaa aactcaacgg
     1441 ggaaagaaaa gagagcaata cctaaaatac caccaattcc tactggtaag aaaaataaga
     1501 cattttcaat aaagttatcc ctcatatgag ctaaaaagga aatcattcgc tcatagattc
     1561 ctaaaatggc tgcgagaaca ccaccggata caccaggtaa aataaaacct agtgcaataa
     1621 tcatgccttt aaaaactctt gaaataaatg aaaccattgt tgcctcctga aaagaatgat
     1681 tttattgtat cataaattta aagaattgct gaca
//
LOCUS       DAXWIQ010000098         1686 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000098 DAXWIQ010000000
VERSION     DAXWIQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.098"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            125..1606
                     /gene="guaB"
                     /locus_tag="ACGEUI_001760"
     CDS             125..1606
                     /gene="guaB"
                     /locus_tag="ACGEUI_001760"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFF8166562.1"
                     /translation="MSNWDTKFLKKGYTFDDVLLIPAESHVLPNEVDLKTKLADNLTL
                     NIPIITAAMDTVTGSKMAIAIARAGGLGVIHKNMSITEQAEEVRKVKRSENGVIIDPF
                     FLTPEHKVSEAEELMQRYRISGVPIVETLANRKLVGIITNRDMRFISDYNAPISEHMT
                     SEHLVTAAVGTDLETAERILHEHRIEKLPLVDNSGRLSGLITIKDIEKVIEFPHAAKD
                     EFGRLLVAAAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIYQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAASDIVFQMLGGIRSGMGY
                     VGAGDIQELHENAQFVEMSGAGLIESHPHDVQITNEAPNYSVH"
BASE COUNT          483 a          292 c          382 g          529 t
ORIGIN      
        1 ctgatagtga cttcagctgt aggaatattt tattgacaaa tgattttagc gtgatatgat
       61 agtaacaata aaaaatagac cagggtaata ttccctaaaa ataaaagaaa agaggattat
      121 ctggatgtca aattgggaca ctaaattttt gaaaaaaggc tacacttttg acgatgtctt
      181 gcttattccg gcagagagtc atgttcttcc aaatgaagtt gatttaaaga caaaactcgc
      241 agacaatttg acattaaata ttcctattat tacagcagct atggatacgg ttactggtag
      301 taaaatggct attgcaattg cgcgtgcggg tggtttaggt gttattcata aaaacatgtc
      361 tattactgag caagcagaag aagttcgcaa ggtaaaacgt tctgaaaatg gtgtcatcat
      421 tgatccattc ttcttaacac ctgaacacaa ggtttcagaa gctgaagaat taatgcagcg
      481 ctaccgtatc agtggtgtgc caattgttga aactttagca aaccgtaagt tggtcggtat
      541 cattactaat cgtgatatgc gttttatttc tgattacaat gcaccaatct ctgaacatat
      601 gactagtgag cacctcgtta cggctgctgt tggtacagat cttgaaactg cagaacgtat
      661 tcttcatgaa caccgtattg aaaaacttcc tttggttgat aatagcggtc gtctgtctgg
      721 tttaatcact atcaaagata ttgaaaaagt aatcgaattc ccacatgctg ctaaagatga
      781 atttggtcgt cttctggtcg ctgctgcggt aggtgttacc tcagatactt ttgagcgtgc
      841 cgaggccttg tttgaagctg gtgcagatgc gattgttatt gacacagctc atggtcattc
      901 ggctggtgtt cttcgtaaaa ttgctgaaat tcgtgctcat ttcccaaatc gtactttgat
      961 tgctggaaat attgccactg ctgaaggtgc gcgtgctctt tatgatgcag gtgttgatgt
     1021 tgttaaagtg ggtattggtc ccggatctat ctgtaccact cgtgtggttg caggtgtcgg
     1081 tgttcctcaa gtgactgcta tttacgatgc tgctgctgtt gctcgtgaat atggcaaaac
     1141 gattattgct gatggtggta tcaagtattc tggcgatatt gttaaggctc ttgcagccgg
     1201 cggtaatgct gttatgcttg gatcaatgtt tgcaggtact gatgaagctc caggtgaaac
     1261 tgaaatctac caaggccgta agttcaaaac ttatcgtgga atgggttcca ttgcagctat
     1321 gaagaaagga tcaagcgatc gttacttcca aggttccgtc aatgaagcta ataagctagt
     1381 tccagaaggt attgaaggcc gtgttgctta taaaggagct gcttcagata ttgtcttcca
     1441 aatgttaggt ggtatccgct caggtatggg ctatgttggc gcaggtgaca ttcaagaatt
     1501 acatgaaaat gctcaatttg ttgaaatgtc aggtgcaggc ttgattgaaa gtcatccaca
     1561 tgatgtccaa atcacaaatg aagcaccaaa ctattctgtg cactgataat ttaatttagt
     1621 aactttaagg gctgacatat tgtgccagct cttttttata ttaaaaaatg ataaaatata
     1681 ataaca
//
LOCUS       DAXWIQ010000099         1655 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000099 DAXWIQ010000000
VERSION     DAXWIQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.099"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(76..381)
                     /locus_tag="ACGEUI_001761"
     CDS             complement(76..381)
                     /locus_tag="ACGEUI_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFF8166563.1"
                     /translation="MTHNHENDHQHEVITLVDEQGNETLFEILLTIDGREEFGKNYVL
                     LVPAGSEEDESGEIEIQAYSFTENEDGTEGDLQPIPEDSDAEWDMIEEVFNSFLDEN"
     gene            complement(393..812)
                     /gene="ruvX"
                     /locus_tag="ACGEUI_001762"
     CDS             complement(393..812)
                     /gene="ruvX"
                     /locus_tag="ACGEUI_001762"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFF8166564.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEEKAEFGFTR
                     LEELVKQYQVEQFVIGLPKNMNNTNGPRVDASITYGNHIEHLFGLPVHYQDERLTTVE
                     AKRMLIEQADISRGKRKKVIDKLAAQLILQNYLNRNF"
     gene            complement(809..1078)
                     /locus_tag="ACGEUI_001763"
     CDS             complement(809..1078)
                     /locus_tag="ACGEUI_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFF8166565.1"
                     /translation="MGFTDETVRFKLDDGDKRQISETLTAVYHSLDEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDVK"
     gene            complement(1191..1589)
                     /gene="spx"
                     /locus_tag="ACGEUI_001764"
     CDS             complement(1191..1589)
                     /gene="spx"
                     /locus_tag="ACGEUI_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HFF8166566.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHKLAYKEQNLGKEPLTKEEILA
                     ILSKTENGVESIVSSKNRYAKALDCDIEELSVSEVIDLIQDNPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNIENTEARLRAAL"
BASE COUNT          497 a          338 c          242 g          578 t
ORIGIN      
        1 cctgcttatt tattcgaaaa taacattaaa aaaatagccc tcacggctac tttttaacat
       61 agtgtcacta acaatttagt tctcatctag aaaactatta aatacctctt caatcatatc
      121 ccattcagca tctgaatcct caggaatcgg ttgtaaatcc ccttcagtac catcttcatt
      181 ttcagtaaat gaataggctt ggatttcaat ttcaccagac tcatcttctt cagatcccgc
      241 tggaaccaag agaacataat ttttaccaaa ctcttcacga ccatcaattg ttaataaaat
      301 ttcaaacaag gtttcatttc cttgttcatc cacaagtgtg ataacttcgt gttggtggtc
      361 attttcatga ttatgtgtca ttttttatct ccttaaaaat tccgatttaa atagttctgt
      421 aaaatgagtt gtgcagctaa tttatcaatg actttttttc gcttaccacg actaatgtca
      481 gcttgctcaa taagcatacg ctttgcctca acagtcgtca atcgttcatc ttgataatgg
      541 actggaagtc caaagaggtg ctctatgtgg tttccataag ttatacttgc atcaacacgt
      601 ggaccattgg tgttattcat attttttggc aatccaataa caaactgttc aacttggtac
      661 tgcttaacaa gctcttctaa acgtgtaaag ccaaattctg ctttttcctc atcaatcttg
      721 ataatttcaa gtccttgggc tgtaaagcca agaggatcac taatagctac accaaccgtt
      781 ttcgagccaa catcaagtcc cattattctc atttaacatc aattccattt ccttgtaaat
      841 agtagcgaac aagttcttct acaatttcat cacgttcata tttacgaatc tgatttcgag
      901 catcattata acgaggaaca taagctgggt ccccactcaa gacataacca acaatttgat
      961 taattggatt ataacccttt tcatcaagtg aatgatagac tgctgttaat gtttcactaa
     1021 tttgtcgttt gtcgccatca tccaacttaa agcggactgt ttcatctgta aatcccatac
     1081 ttacaccttc tttctaaaat taagtgctat gctttattat agcttatttc tattaaataa
     1141 acaagaattt caagcgaaga aggtatttaa aaaaagctct acaagagctt ttagagtgca
     1201 gcacgtaatc gtgcttcagt attttcaata ttacgaatag agcgaggtaa aaaagcacga
     1261 atatcgtctt ctttgtagcc tacttgcaga cgtttatcat caatcaaaat gggactttta
     1321 aggatgcgcg ggttatcttg aatcaaatca ataacctcac taacacttaa ttcttcaata
     1381 tcgcaatcga gagctttggc ataacgattt ttagatgaaa caatgctctc cactccattt
     1441 tcggtttttg ataaaattgc taaaatttct tctttagtta gcggttcttt tcctaagttc
     1501 tgttctttat aagcaagctt atgggcattt agccaagttt tggctttctt gcagctcgta
     1561 caacttgaaa tcgtgtaaat tttaatcatg ttgctgactc actttctatt tcgtgatagc
     1621 tttattgtag cacagtttct tatctataaa tatag
//
LOCUS       DAXWIQ010000100         1615 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000100 DAXWIQ010000000
VERSION     DAXWIQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.100"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..674
                     /locus_tag="ACGEUI_001765"
     CDS             <1..674
                     /locus_tag="ACGEUI_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8166567.1"
                     /translation="AIDDDKEILKLMKTALEIENYHVITCQEIELPIVFDDFKGYDLI
                     LLDIMMPNISGTEFCYKIREEVHSPIIFVSALDGDNEIVQALNIGGDDFIVKPFSLKQ
                     FVAKVNSHLKREERAKIKNEAEERVKRSFPPIEIYLEERMLYIDKQPLFLTYREYEIL
                     ELLSRHPYKVFTKEEIYEQVYSDEASALFHSISEYIYQIRMKFSSFGINPIKTIRGIG
                     YKWDV"
     gene            667..>1615
                     /locus_tag="ACGEUI_001766"
     CDS             667..>1615
                     /locus_tag="ACGEUI_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFF8166568.1"
                     /translation="MFKKYSIKNRVWRAVVEIVFGVCITVLMIAIISLSFSKLNIVTS
                     HNVGEEFYIKDKQSIKQLNNYMKTLGLDYVVFDRKTDKAMEGKYLSKEFSLFNEVAEE
                     KNNLTFNSVHYDLYTNINYNIVIRYNEIPEFSNHYLRNVSYNMLTFYILGIGTSISIV
                     VALTRFVKEISLNFKEIKKLANKMGIEVLSENENYSKIIEFDDILRTLHIKGDNLKSL
                     IEREILEKQDLSFQIAALSHDIKTPLTVLKGNLELLELTTLNKNQEGYIVSMNNSISV
                     FEGYFNSLISYTRMLSEDRSVKLILVEELLSELHFEVDDL"
BASE COUNT          586 a          182 c          293 g          554 t
ORIGIN      
        1 tagccataga tgatgataaa gaaattctga agcttatgaa gactgctttg gagattgaaa
       61 attatcatgt tataacctgt caagagatag aattaccaat agtttttgat gattttaaag
      121 gatatgattt gatcttattg gatatcatga tgcctaatat aagtggaact gagttttgtt
      181 ataaaattcg ggaagaagtt cattctccaa ttatttttgt tagcgcttta gatggcgata
      241 atgaaattgt ccaagcttta aatatagggg gagacgattt tattgtgaaa ccatttagct
      301 taaaacaatt cgtagctaaa gttaactctc atttgaagag agaagagaga gcaaagataa
      361 agaatgaggc tgaggagaga gtgaagcgta gttttccacc tatagaaatc tatctagagg
      421 aacgtatgtt atatattgat aaacagccgt tattcctgac ttatagagag tacgaaattt
      481 tagaattact gtcacgtcat ccgtataaag ttttcacaaa agaagagata tatgaacaag
      541 tatatagcga tgaagcttca gcattgtttc attctatttc agaatatatc tatcagatta
      601 gaatgaaatt ttcaagtttt ggaattaatc caataaaaac tattcgcggg attggttata
      661 aatgggatgt ttaaaaaata ttcaataaaa aatcgtgttt ggcgcgccgt cgttgaaatt
      721 gtctttggag tttgtatcac tgttttaatg attgcgataa tatcgctttc tttttcaaag
      781 ttaaatatag tgacatctca taatgtggga gaagaatttt atattaaaga taaacagtca
      841 atcaaacagt tgaacaatta tatgaagaca ttgggattag attatgttgt ttttgataga
      901 aaaacagata aagctatgga aggaaaatat ttatctaaag aattttcttt atttaacgaa
      961 gttgcagaag aaaaaaataa tctgactttt aattctgtac attatgattt atatactaat
     1021 atcaattata atattgttat aagatataat gagataccgg agttctctaa ccattacctt
     1081 agaaatgttt catataacat gttgacattt tatattctgg gaataggaac gagtatcagc
     1141 attgtagttg ctttaacacg gtttgtaaaa gaaatttctt tgaatttcaa ggaaatcaag
     1201 aagttagcaa ataaaatggg gatagaagtc ctttctgaaa atgaaaatta ttctaaaata
     1261 atcgagtttg atgatatact tagaacttta catattaaag gtgataattt gaaaagtttg
     1321 attgagcgag aaatattgga aaagcaagat ctatcctttc aaattgcagc actttcgcat
     1381 gatataaaaa cacctttaac ggttttaaaa ggaaatcttg agttattaga attgactact
     1441 ttaaataaaa atcaggaagg ttatattgta tctatgaaca atagtatttc tgtttttgaa
     1501 ggctatttca attctttaat atcttataca cgcatgcttt cagaggatag atcagtgaaa
     1561 ctaattttag ttgaggagct attaagtgag ttacattttg aagtcgatga cttat
//
LOCUS       DAXWIQ010000101         1607 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000101 DAXWIQ010000000
VERSION     DAXWIQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.101"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            25..945
                     /gene="coaA"
                     /locus_tag="ACGEUI_001767"
     CDS             25..945
                     /gene="coaA"
                     /locus_tag="ACGEUI_001767"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFF8166569.1"
                     /translation="MSNEFINFEKISRESWKTLHQKAKALLTQEELKSITSLNDNISI
                     NDVIDIYLPLINLIQVYKIAQENLSFSKSLFLKKDIQLRPFIIGISGSVAVGKSTTSR
                     LLQLLLSRTHPNSQVELVTTDGFLYPNQFLIEQGLLNRKGFPESYNMELLLDFLDSIK
                     NGQTAFAPVYSHDIYDIIPNQKQSFNNPDFLIVEGINVFQNQQNNRLYMSDYFDFSIY
                     IDADSSHIETWYIERFLSILKLAKRDPHNYYAQYAQLPRSEAIAFARNVWKTVNLENL
                     EKFIEPTRNRAELILHKSADHKIDEIYLKK"
     gene            999..1247
                     /gene="rpsT"
                     /locus_tag="ACGEUI_001768"
     CDS             999..1247
                     /gene="rpsT"
                     /locus_tag="ACGEUI_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFF8166570.1"
                     /translation="MEVKTLANIKSAIKRAELNVKANEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSSIDKAESKGLIHKNKASRDKARLAAKLG"
     gene            complement(1372..>1607)
                     /locus_tag="ACGEUI_001769"
     CDS             complement(1372..>1607)
                     /locus_tag="ACGEUI_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8166571.1"
                     /translation="TTDKNLLISVIDNGPGITDEEKKKIFDRFYRVDKARTRQTGGFG
                     LGLALAQQIVMSLKGNITVKDNDPKGSIFEVKL"
BASE COUNT          560 a          303 c          231 g          513 t
ORIGIN      
        1 actgtcaaat gattaggagc taccatgtca aatgaattta ttaactttga aaaaatttct
       61 agagagtctt ggaaaacatt acatcaaaag gcaaaagctc tcctcacaca agaggaactt
      121 aaaagtatta ctagcttaaa tgataacatc agtattaacg atgtcattga tatctacctt
      181 ccattaatta atctgatcca ggtttacaaa attgcgcaag aaaatctttc tttttctaaa
      241 agcctttttc taaaaaaaga cattcaatta aggcctttta ttattggaat ttctggttca
      301 gtagctgttg gtaaatcaac aactagtcgt cttttacaac ttttattatc acgaacgcac
      361 cccaatagtc aagtcgaact ggtaacgact gatggttttt tatatcctaa tcagtttctt
      421 attgaacaag gactgttaaa tcgtaaagga tttccagaat cttataacat ggaacttctc
      481 ttagattttc tagattccat caaaaatggt caaactgctt ttgcacccgt ctattctcat
      541 gatatttacg atattatccc aaatcaaaaa caatcattta ataaccctga cttccttatc
      601 gtcgaaggta ttaacgtttt tcaaaatcaa caaaacaatc gtttatatat gagtgattac
      661 tttgattttt ctatttatat tgatgcagac agcagtcaca tcgaaacctg gtacattgaa
      721 cggtttctaa gcattttaaa attagcaaaa cgtgaccctc ataattatta tgcccaatat
      781 gcacaattac ctcgatctga ggctatagca tttgcgcgca acgtttggaa aaccgttaat
      841 ttagaaaatc ttgaaaaatt cattgaacct acacgtaacc gtgctgaatt aattttacat
      901 aaaagtgctg atcataaaat tgatgaaatt tacctcaaaa agtgataaag ggttgccaaa
      961 cgcatatatt ttctatataa taatgtagtt agattaattt ggaggtgaaa acattggcaa
     1021 atattaaatc agctattaaa cgcgctgaac ttaacgttaa agcaaacgaa aaaaactcag
     1081 cacaaaaatc agctatgcgt actgctatca aagcatttga agctaaccca tctgaagagc
     1141 ttttccgcgc tgcttcttca agcatcgata aagctgaatc aaaaggtttg attcacaaaa
     1201 acaaagcaag ccgcgataaa gcacgccttg ctgcaaaact tggctaataa atatccaatg
     1261 aaaactccag ctggagtttt tttatacggt aaaaactcaa aaaatacaat aaagcgttga
     1321 cagttaaaaa taactttaaa agggctgacc ttattggtcg ccctttttag cttatagttt
     1381 gacttcaaaa atactacctt taggatcatt atcctttact gtaatatttc cttttaaaga
     1441 catcacgatt tgctgagcta aagccaaccc caagccaaat ccacctgttt gccgtgttct
     1501 agctttgtca actcgataaa aacgatcaaa aatctttttc ttttcttcat ctgttatccc
     1561 tggaccatta tcaataacag aaattaataa atttttgtcc gttgttt
//
LOCUS       DAXWIQ010000102         1490 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000102 DAXWIQ010000000
VERSION     DAXWIQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.102"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            93..1163
                     /gene="xerS"
                     /locus_tag="ACGEUI_001770"
     CDS             93..1163
                     /gene="xerS"
                     /locus_tag="ACGEUI_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFF8166572.1"
                     /translation="MRRELLLEKIETYKAIMPWYVLDYYQSKLAVPYSFTTLYEYLKE
                     YKRFFDWLMDADLTQAPKIADIDLSTLEHLTKKDLEAFVLYLRERPSLNTYSTKEGLS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGDETLAFLDYVDKEYEQKLSNRAKSSFRKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDVNLNMMIIEVIRKGGKRDSVNVAGFAKGYLESYLAVRQRRYKAEKQDLAFFLTEY
                     RGVPNRMDASSIEKMVGKYSEDFKIRVTPHKLRHTLATRLYDATKSQVLVSHQLGHSS
                     TQVTDLYTHIVNDEQKNALDNL"
BASE COUNT          476 a          240 c          282 g          492 t
ORIGIN      
        1 ctcttgtaga gaatgaatgt cataaaatac atgagacaat ttacgaaaat tcatttttga
       61 aaaaattgtt attgttaagg aattatgatg atatgagacg cgaattatta ctagaaaaaa
      121 ttgaaaccta caaggctatc atgccctggt atgttttaga ttattaccaa tccaaattag
      181 ctgttccata cagctttacc accttatatg agtatttaaa agaatacaaa cgcttttttg
      241 attggctgat ggatgctgat ttaacgcagg cgccaaagat tgctgatatt gacttgagca
      301 cgcttgagca ccttaccaag aaggatttag aggcctttgt gctttatttg cgggaacgac
      361 cttctctcaa cacctattcc accaaagagg ggctttctca aaccactatt aatcggacct
      421 tgtctgcctt atcaagcctt tacaagtact tgactgaaga ggttgaaaat gaccaagggg
      481 agccttattt ttatcggaat gttatgaaaa aagtgtcaac aaaaaagaaa aaagaaaccc
      541 tggcttccag agcggaaaat attaagcaga aactgttttt aggtgatgaa actctagctt
      601 tcttggatta tgtggataag gaatatgagc aaaaattgtc taatcgtgcc aaatcttctt
      661 tccgtaaaaa taaggagagg gatttggcta ttattgctct actacttgct tctggtgttc
      721 gtttgtctga agcagtgaat cttgatttaa aagatgtcaa tctaaatatg atgattatcg
      781 aagttattcg caaaggcgga aaacgtgatt cagtcaatgt agcaggtttt gcgaaaggtt
      841 atttggagtc ttatttagct gttcgtcaga gacgttacaa ggctgaaaaa caggatcttg
      901 ctttcttttt aacagaatac cgtggcgttc caaatcgaat ggatgcttct agtatagaaa
      961 agatggttgg caagtactct gaggatttta aaattcgtgt gactcctcac aaattgcgtc
     1021 atacgctagc gacaagacta tatgatgcta ctaaatctca ggtgttagtt agtcatcaat
     1081 taggccattc ctctactcaa gtcacggatc tttataccca tattgtcaat gatgagcaaa
     1141 aaaatgcttt agataacctt taatgttaca taattgtttt tatgtaacat taaagataaa
     1201 aatagcttca aaattaactt taatagatgg ctactattaa ccgtgacagt ttggtgtcag
     1261 gccgttttcc aaactactca agccacttag aaaaaggaat attactgcta actttgtccc
     1321 ttctttgaat gacttttttg tgatgtttat agattaaatc tagtagggtt ctcatcaaaa
     1381 agatgagata agcctgtttt tagtatgtca gtacttttat ttagtatgat aatacgttat
     1441 tttgtaaagt agcttttagg attattcttg ttaaactact taatgtcatt
//
LOCUS       DAXWIQ010000103         1368 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000103 DAXWIQ010000000
VERSION     DAXWIQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.103"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            44..1063
                     /gene="ideS"
                     /locus_tag="ACGEUI_001771"
     CDS             44..1063
                     /gene="ideS"
                     /locus_tag="ACGEUI_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin G-degrading enzyme IdeS"
                     /protein_id="HFF8166573.1"
                     /translation="MRKRCYSTSAAVLAAVTLFVLSVDRGVIADSFSANQEIRYSEVT
                     PYHVTSVWTKGVTPPANFTQGEDVFHAPYVANQGWYDITKTFNGKDDLLCGAATAGNM
                     LHWWFDQNKDQIKRYLEEHPEKQKINFNGEQMFDVKEAIDTKNHQLDSKLFEYFKEKA
                     FPYLSTKHLGVFPDHVIDMFINGYRLSLTNHGPTPVKEGSKDPRGGIFDAVFTRGDQS
                     KLLTSRHDFKEKNLKEISDLIKKELTEGKALGLSHTYANVRINHVINLWGADFDSNGN
                     LKAIYVTDSDSNASIGMKKYFVGVNSAGKVAISAKEIKEDNIGAQVLGLFTLSTGQDS
                     WNQTN"
     gene            1181..>1368
                     /locus_tag="ACGEUI_001772"
     CDS             1181..>1368
                     /locus_tag="ACGEUI_001772"
                     /inference="COORDINATES: protein motif:HMM:NF014875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin beta-grasp domain-containing protein"
                     /protein_id="HFF8166574.1"
                     /translation="MDKDIVTIQEFDFKIRKFLMESKEIYLTKSPYIRGSLEIHSKNR
                     KHEKINLYDAKPNSTRSDV"
BASE COUNT          494 a          226 c          254 g          394 t
ORIGIN      
        1 tataatttat ttatcataaa attatataga aaaaggtatt cttatgagaa aaagatgcta
       61 ttcaacttca gctgcagtat tggcagcagt gactttattt gttctatcgg tagatcgtgg
      121 tgttatagca gatagttttt ctgctaatca agagattaga tattcggaag taacacctta
      181 tcacgttact tccgtttgga ccaaaggagt tactcctcca gcaaacttca ctcaaggtga
      241 agatgttttt cacgctcctt atgttgctaa ccaaggatgg tatgatatta ccaaaacatt
      301 caatggaaaa gacgatcttc tttgcggggc tgccacagca gggaatatgc ttcactggtg
      361 gttcgatcaa aacaaagacc aaattaaacg ttatttggaa gagcatccag aaaagcaaaa
      421 aataaacttc aatggcgaac agatgtttga cgtaaaagaa gctatcgaca ctaaaaacca
      481 ccagctagat agtaaattat ttgaatattt taaagaaaaa gctttccctt atctatctac
      541 taaacaccta ggagttttcc ctgatcatgt aattgatatg ttcattaacg gctaccgcct
      601 tagtctaact aaccacggtc caacgccagt aaaagaaggt agtaaagatc cccgaggtgg
      661 tatttttgac gccgtattta caagaggtga tcaaagtaag ctattgacaa gtcgtcatga
      721 ttttaaagaa aaaaatctca aagaaatcag tgatctcatt aagaaagagt taaccgaagg
      781 caaggctcta ggcctatcac acacctacgc taacgtacgc atcaaccatg ttataaacct
      841 gtggggagct gactttgatt ctaacgggaa ccttaaagct atttatgtaa cagactctga
      901 tagtaatgca tctattggta tgaagaaata ctttgttggt gttaattccg ctggaaaagt
      961 agctatttct gctaaagaaa taaaagaaga taatattggt gctcaagtac tagggttatt
     1021 tacactttca acagggcaag atagttggaa tcagaccaat taaagcgtat caataagaaa
     1081 gattaccttc aacagatgat ggtattctaa ggactaatac tattgcaggg aattttttta
     1141 tagatgggca acagcaaaaa cattaataaa tcctataaaa atcgataagg atattgttac
     1201 gattcaagag ttcgatttta aaataagaaa atttttgatg gaatcaaaag aaatttattt
     1261 gactaagtct ccttatataa gggggagttt agaaattcac agtaaaaata ggaaacatga
     1321 aaaaatcaat ttatatgacg caaaacccaa tagcaccaga tctgatgt
//
LOCUS       DAXWIQ010000104         1323 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000104 DAXWIQ010000000
VERSION     DAXWIQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.104"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            111..>1323
                     /locus_tag="ACGEUI_001773"
     CDS             111..>1323
                     /locus_tag="ACGEUI_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFF8166575.1"
                     /translation="MKIVQGVQLHLIKTKQFKTNHITFRFSGDLNQKTVAKRVLVAQM
                     LATANECYPTVRQFREKLARLYGASLSTNVLTKGLVHIVDIDITFIQDRYACNGEKIL
                     DEMIQFLKDILFSPLLSIAQYQPKVFETEKNNLINYIESDREDSFYYSSLKVKELFYC
                     NKNLQMSEYGSPELIAKETAYTSYQEFHKMLNEDQIDIFILGDFDDYRVVQLIHQFPL
                     DNRNKNLNFFHLQNSVNIIKESIEKRAVHQSILQLAYHFPSVFGQRDYYALVLLNGLL
                     GSFAHSRLFIKIREEEGLAYSIGCRFDSYTGLFEIYTGIDSQHRTKTLQLIIQELNAI
                     KMGRFSEQLIKKTRSMLLNNALLSEDYNKNIIERIYRSSYIDSSYSIKNWIKGVNEVN
                     KADIIKVANLLK"
BASE COUNT          482 a          198 c          207 g          436 t
ORIGIN      
        1 ttttactaac actagaatcc cgacaattaa gtcaaaaaaa gttatcactc acctaacctg
       61 aataaaaatg gtataatcgt aaggatcaca ctatttatta aggaaacatt atgaaaattg
      121 ttcagggagt acaacttcat ctgattaaga ccaaacagtt taaaaccaat catattacct
      181 ttcgtttttc aggtgatttg aaccaaaaaa cagtagcaaa aagagtctta gtggcacaga
      241 tgttagcaac agctaatgag tgttatccaa ctgttaggca gtttagagaa aaattagcac
      301 gactttatgg agctagcttg tcaactaacg tgttaacaaa agggttagtt catattgtgg
      361 atatcgatat tacttttatt caagatagat atgcttgcaa tggggaaaaa atactagatg
      421 aaatgattca gtttttaaaa gatatcttgt tctcacccct attatccatc gcccagtatc
      481 agcctaaagt ttttgagact gaaaaaaata atttgattaa ctacatcgag tcagatagag
      541 aggattcttt ttactacagc tctcttaagg taaaagaatt gttttattgt aataagaatc
      601 tacaaatgtc agaatatggt agcccagaac taattgcaaa agagactgcc tatactagct
      661 atcaagaatt tcataaaatg ctcaatgaag atcagattga tattttcatt ttaggagatt
      721 ttgatgatta tcgcgttgtt cagctaattc atcaatttcc acttgataac cgaaataaaa
      781 acctaaattt ctttcattta caaaattctg tcaatattat taaagaatct attgaaaaaa
      841 gagcagttca tcaatctatt ttacaattag cttatcactt cccttcagtt tttggacaac
      901 gtgactatta tgctttagtt cttttaaatg ggttgttagg ctcttttgca cattcccgtc
      961 tctttataaa aattagagaa gaagaaggac ttgcttatag tatagggtgt cgttttgata
     1021 gttatacagg attatttgaa atttatactg gaattgacag tcaacatcgc actaaaacgt
     1081 tacaactaat catccaagaa ttaaatgcca ttaaaatggg acgattctca gaacaattga
     1141 ttaaaaagac cagatcgatg ttgttgaata atgccttatt atcagaagat tataataaaa
     1201 atataattga aagaatatac agatcttctt acattgattc ttcttattct attaaaaatt
     1261 ggattaaagg ggttaatgaa gttaataaag cagatatcat taaagtagct aatcttttaa
     1321 agc
//
LOCUS       DAXWIQ010000105         1283 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000105 DAXWIQ010000000
VERSION     DAXWIQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.105"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(16..258)
                     /locus_tag="ACGEUI_001774"
     CDS             complement(16..258)
                     /locus_tag="ACGEUI_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFF8166576.1"
                     /translation="MTRQEIFERLINLIQKQRSYLSVAITEQTHLKNDLAVDSIELVE
                     FIINVEDEFHIAIPDEDVEDMVFMRDILDYLVQRLT"
     gene            complement(251..1258)
                     /gene="plsX"
                     /locus_tag="ACGEUI_001775"
     CDS             complement(251..1258)
                     /gene="plsX"
                     /locus_tag="ACGEUI_001775"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFF8166577.1"
                     /translation="MKRIAIDAMGGDNAPKAIVEGVNQAIEAFSDIEIQLYGDQTKIN
                     SYLIQSDRVAIIHTDEKIMSDDEPAKAVRRKKKASMVLAAKAVKEGKADAIISAGNTG
                     ALLAVGLFVVGRIKGVDRPGLLSTIPTVTGLGFDMLDLGANAENTAKHLHQYAILGSF
                     YAKNVRGIANPRVGLLNNGTEETKGDPLRKATYELLTADNTISFVGNVEARELMSGVA
                     DVIVSDGFTGNAVLKSIEGTAISIMGQLKQIINSGGIKTKIGASLLKSSLYEMKKTLD
                     YSSAGGAVLFGLKAPVVKSHGSSDVKAIFSTIKQVRTMLDTNVVGQLVEEFAKETQVN
                     D"
BASE COUNT          391 a          286 c          176 g          430 t
ORIGIN      
        1 tttttattat tctaactaag tcaaacgctg aactaaataa tctaaaatgt ctctcataaa
       61 taccatatcc tcaacatctt catcaggaat agcaatatga aattcatctt caacattaat
      121 aataaattca actagttcaa tagagtcaac cgctaaatca ttttttaaat gtgtttgttc
      181 tgtaatagct acactcaaat agcttctttg cttctgaatt aagttaatca gcctttcaaa
      241 aatctcctgt ctagtcattt acttgagtct ccttagcaaa ttcttctact aattgtccga
      301 ccacgttagt gtctagcatc gtacgtactt gttttatcgt tgaaaaaata gcttttacat
      361 cactagaacc atgagacttt acaacaggtg cctttaaacc aaataaaact gcacctccag
      421 cactagagta gtcaagagtc ttcttcatct cgtaaagact acttttaagt agactagctc
      481 caattttagt cttaataccg ccgctattaa ttatctgttt cagttgcccc ataatgctaa
      541 tagctgttcc ttctattgat ttcaacactg cgtttcccgt gaaaccatct gatacaataa
      601 catctgcaac acctgacata agttctcgcg cctcgacatt accaacaaaa gatattgtat
      661 tatctgctgt taaaagttca taagtggcct tacgaagggg atctcctttt gtttcttctg
      721 taccattgtt taatagacca acacgtggat tagcaatccc acgtacattc ttagcataga
      781 aagagcctaa aatagcatat tgatgtaaat gttttgccgt attttcagca ttagctccaa
      841 gatccaacat atcaaatcct aatccggtta ctgtgggaat agttgataat aatcctggac
      901 gatcaactcc cttgatacgc cccacaacaa acaaacctac agctaaaaga gcacctgtat
      961 tccctgctga aataatggca tctgcttttc cttctttcac tgccttagct gctaaaacca
     1021 ttgaagcctt ctttttacgg cgaacagctt ttgcaggttc atcatctgac ataatttttt
     1081 catctgtatg aataatagca acacgatctg attgaataag ataggagtta atctttgtct
     1141 gatcaccgta aagttgaatt tcgatatctg aaaatgcttc tattgcttga ttaacaccct
     1201 caacaatagc tttaggagcg ttatctccac ccatagcatc tattgcaatt ctcttcattt
     1261 ccactccttt aattatatct tgt
//
LOCUS       DAXWIQ010000106         1123 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000106 DAXWIQ010000000
VERSION     DAXWIQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.106"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(<1..1122)
                     /locus_tag="ACGEUI_001776"
     CDS             complement(<1..1122)
                     /locus_tag="ACGEUI_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8166578.1"
                     /translation="MYRIGDVMFDFANNTFLAGLNPASLSLVAVYQSLESVIGVLFNL
                     FGGVIADSFKRKKIIITTNILCGTACLVLSFLTKEQWLVYAIVLTNVILAFMSAFSSP
                     SYKAFTKEIVKKDSISQLNSLLETTSTVIKVTVPMVAIFLYKLLGIHGVLLLDGLSFL
                     IAALLISFILPVNDEVVIKEKVTIREIFNDLKIGFKYVYSHKSIFIITVLSALVNFFL
                     AAYNLLLPYSNQMFGEISTGLYGTFLTAEAIGGFIGAILSGFVNKELSSMRLILFLSL
                     SGLMLMLAPPFYIMFHNAIILALSPALFSLFLSIFNIQFFSLVQKDVDNDFLGRVFGI
                     IFTITILFMPIGTGFFSVALNPNNSFNLFIIGSCITTLSL"
BASE COUNT          449 a          185 c          165 g          324 t
ORIGIN      
        1 taatgacaat gtagtaatgc acgaaccaat aataaaaaga ttaaagctat tgttaggatt
       61 taatgccacc gagaaaaagc cagtcccaat aggcataaaa agaatagtta ttgtaaagat
      121 aataccaaac actctcccta aaaaatcgtt atcaacatct ttctggacaa gagaaaaaaa
      181 ttgaatatta aagatggaaa gaaataaact gaataaagct ggtgacaaag ctaaaataat
      241 tgcattatga aacataatat aaaatggggg agctaacatt aacatcagtc ctgataaaga
      301 tagaaacaat atcaatctca tacttgataa ttctttattc acaaaaccac ttaatattgc
      361 cccaataaat ccaccaattg cttctgctgt taaaaaagtg ccataaagtc cagttgaaat
      421 ttctccaaac atttgattac tatagggcaa caataaatta taagcggcta gaaaaaaatt
      481 aacaagtgca gaaagaacgg taataataaa gatggactta tggctgtaaa catatttaaa
      541 tcctattttt aaatcgttaa atatttctct gattgtcacc ttctccttta tcaccacttc
      601 gtcattaaca ggtaaaataa aggaaattag taaagcagct attagaaatg atagtccatc
      661 caatagtaat acaccgtgta taccaagtag cttatataag aaaattgcta ccataggaac
      721 tgttactttg attacagtac ttgttgtctc tagcaatgaa ttaagttgtg atatactatc
      781 ttttttaaca atttcttttg taaatgcttt ataggatgga ctagaaaaag cactcataaa
      841 tgccaagata acattagtta gtacaatggc ataaaccaac cattgctctt ttgttaggaa
      901 cgagagtact aagcaagctg tgccacataa gatatttgta gtaataataa tttttttccg
      961 cttgaaactg tctgcaatga ctccaccaaa taaattaaat agaacaccta tgacactttc
     1021 tagagactga tatactgcaa ctaatgatag agacgctgga ttaagcccag caagaaaagt
     1081 attgttagca aaatcaaaca taacatcgcc aattcgatat ata
//
LOCUS       DAXWIQ010000107         1116 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000107 DAXWIQ010000000
VERSION     DAXWIQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.107"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..>1116
                     /locus_tag="ACGEUI_001777"
     CDS             <1..>1116
                     /locus_tag="ACGEUI_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HFF8166579.1"
                     /translation="KEIEKALSDFNYRIEIIVCTSSNQNISSNSQEVIDDFLQGVNSD
                     GSDDVVTYRHIKLNDVYDHMANQGVAPKIDFEDVVVHNWGRPTLYNDSYKVYYGWLSA
                     TDIVNWKEQYGDRIFEKNIRNFKQNTDVNNGIKIISEKVEKNLKGVSTTDYINLKLIG
                     ASIINGAQTTGSLFEAYHSEDIDLTNVSVQVQIIVLGEDIDNIGPKITKLSNTQNRIE
                     NKDFAAQDKEQERLMKDLAIDGKQYVYRQGVELPNSDEGCDLDSATVALGCYLDDVAI
                     STQMKRAYGSIFDNTNKPPYKLIFNSGTSAYKLWNCVEVYRELQNIEGEYQQDPNNQS
                     KKLMSIHGNRFILHLIFQKLKNNPSLDFENGYITTFP"
BASE COUNT          427 a          137 c          205 g          347 t
ORIGIN      
        1 aaaagaaata gaaaaagctt tatcagattt taattatcga atagaaatta tagtttgtac
       61 ttcctctaat caaaatatat cttcaaatag tcaagaagta atagatgatt ttttgcaggg
      121 tgtaaattct gatggtagtg atgatgtagt aacatacaga catataaaac ttaatgatgt
      181 ttatgatcat atggctaatc aaggagtggc tcctaaaata gatttcgagg acgttgtcgt
      241 tcataattgg ggcagaccta cattatacaa tgacagttat aaagtctatt atggatggtt
      301 atctgctact gatatagtta actggaaaga gcaatatgga gatcgaatat ttgaaaagaa
      361 tattcggaac tttaagcaaa atacggatgt taataacgga ataaaaataa tttctgaaaa
      421 agttgaaaaa aatctaaaag gtgtttcaac aacggattat atcaatttaa aattgattgg
      481 agcaagtata attaatggag cgcaaacaac gggttcttta tttgaagcat accattctga
      541 agatattgat ttgacaaatg tttcagtcca agttcaaata atagtattag gagaagatat
      601 tgataatatc ggacctaaaa taactaagtt atcgaatact caaaatagaa tagaaaataa
      661 agattttgct gctcaggata aagaacaaga aagattgatg aaagatcttg caattgatgg
      721 taaacagtat gtgtatcgtc aaggtgtcga gctacctaat tctgatgaag gttgtgatct
      781 ggatagtgca acagtggctc taggttgcta tttagatgat gttgcaattt ctacacagat
      841 gaagagagca tatggtagca tttttgacaa tactaataaa cctccatata agctaatctt
      901 taattccgga acttctgcat ataaattatg gaattgcgtt gaagtgtatc gcgaattaca
      961 aaacatagaa ggggagtatc aacaagatcc aaataatcaa tctaaaaagt tgatgtctat
     1021 tcatggaaat agatttattc ttcatttgat tttccaaaag ttaaaaaata acccaagttt
     1081 agatttcgag aatggatata ttacaacttt tccaaa
//
LOCUS       DAXWIQ010000108         1113 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000108 DAXWIQ010000000
VERSION     DAXWIQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.108"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            334..573
                     /locus_tag="ACGEUI_001778"
     CDS             334..573
                     /locus_tag="ACGEUI_001778"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8166580.1"
                     /translation="MHNHLFHYTRQVRRHVKVITIGILSAHYYEITMLFLQAKIILDC
                     FHIPQRLRQAITRFRIRIINTSAIKPIAYRTLKLY"
     gene            complement(1019..1106)
                     /locus_tag="ACGEUI_001779"
     tRNA            complement(1019..1106)
                     /locus_tag="ACGEUI_001779"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1070..1072),aa:Ser,seq:gct)
BASE COUNT          393 a          232 c          131 g          357 t
ORIGIN      
        1 agtcagacct tgttttttta taagctaact atcgaaaact ggtcatctat aaatcttagt
       61 attccatcac tcaatcacaa ttcataaaga ctcgcaaact ttcacaactt ttaattctat
      121 actttctgta gtaggtaaat ccacctcaaa gattatagag tctttttaaa catatataaa
      181 aagttccata tgacgatcta taatgaaaag cgaccaaact atcatttcag aaagactcat
      241 atagaataat ccgattatag tataatttca ttaggaatct aaaaccttag catcacggtt
      301 ttaaattatg taaatgctgg ggacgcaaaa aacattcata atcatctctt tcattatacc
      361 agacaggtta gaaggcatgt caaagttatt accataggca tactcagtgc ccactactat
      421 gagattacta tgctttttct acaggctaaa attatcctcg attgttttca tatcccccaa
      481 cgtcttagac aagctataac tcgctttcgt atccgaatca taaatacttc tgctataaaa
      541 cctatcgcgt atagaactct caaactttac tgaaaactca tacagcatga ttcatggaag
      601 ctcatcaata atagctttat tgcccaattt ttcgtacgcc cataactaac atctagaaaa
      661 gaaaagctct tactcacaag atctctgaat ttcaataatt attcctaact ctatcaactc
      721 ttgctcttcc catttttcag acaattttta aaatttttct aaaagaaaag aacaagatta
      781 gcaatatact taaactcaac tactctaagg ccaaattaga aacagtcaac aaccttatca
      841 aagccatgaa gttaaatgtt ttactcctct acagtagtca aagagcctat cattttagtt
      901 atcgaaataa tgaacaattt aaatattcta tttagttggt tttgaagatt acaaaaaaag
      961 acgataaccg tcttctttcc cctttattta caattcatcc attaatatgt ttacaaaacg
     1021 gagaataagg gattcgaacc cttgcgccag ttacccgacc taacgattta gcaaaccgtc
     1081 ctcttcagcc tcttgagtaa ttctccaaat ata
//
LOCUS       DAXWIQ010000109         1018 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000109 DAXWIQ010000000
VERSION     DAXWIQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.109"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(<1..>1018)
                     /locus_tag="ACGEUI_001780"
     CDS             complement(<1..>1018)
                     /locus_tag="ACGEUI_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFF8166581.1"
                     /translation="NKLKKEILSDNYNHFFHFFAVFTGIFVIMTIIILQIMRFGVYSS
                     VDSSLVSVSNNASSYANRTMARISSFYFDTENNIIKALPDSDSSKLLGTPAANTDIIL
                     FSANGTILNAFDAFSNYQNFHLDKRRLGSIETTSLMNFYGQEEKYHTITVGVHIKNYP
                     AVAYMMAVVNVEQLDRANERYERIIIIVMSVFWLISILASIYLAKWSRKPILESYEKQ
                     KMFVENASHELRTPLAVLQNRLESLFRKPNETILENSEHLASSLDEVRNMRILTTNLL
                     NLARRDDGINPQWTHLDTDFFNAIFENYELVAKEYGKIFYFQNQVNRSLRMDKALLKQ
                     LITIL"
BASE COUNT          356 a          183 c          142 g          337 t
ORIGIN      
        1 taaaatcgtt attaattgtt ttagtaaagc cttatccatt cttaacgatc tattgacttg
       61 gttctgaaaa taaaatattt ttccatattc tttagcaact agttcataat tctcaaaaat
      121 agcattaaaa aaatctgtat ctaaatgagt ccactgtgga ttaatgccat catctcgtct
      181 tgctaaattt aataaattag ttgttaagat gcgcatgttg cgaacctcgt ctaaactaga
      241 agcgagatgc tcactatttt ctaatatcgt ttcgttgggc ttacgaaaaa gcgattccag
      301 acgattctgt aagaccgcca aaggggtcct taattcatga ctagcatttt caacaaacat
      361 tttttgtttt tcatagcttt ctaaaatagg ttttctgctc cacttggcta aataaatact
      421 tgctaaaata gaaattagcc aaaaaacact cataactata ataataatgc gctcataacg
      481 ttcattagcg cggtctaatt gttccacatt tactactgcc atcatatagg caactgcagg
      541 ataatttttg atatgaaccc ctacagttat cgtatggtat ttttcttctt gtccataaaa
      601 attcattaaa ctggtggttt caatactccc caaccggcgt ttatctaaat gaaaattttg
      661 atagttagaa aacgcatcaa aagcatttaa aattgttcca ttagcactaa acaaaatgat
      721 atctgtatta gctgcaggcg ttcctaataa cttagaacta tctgaatcag gcagcgcctt
      781 aataatgtta ttttcagtat caaagtaaaa agaagatatt ctagccatcg tacgattagc
      841 atagctactt gcattattac taacagaaac taaactactg tcaactgacg aataaacgcc
      901 aaaccgcata atctgtaaga taataatagt cataatgaca aaaatacctg taaaaaccgc
      961 aaaaaaatga aaaaagtggt tatagttatc tgataaaatc tcttttttta atttattc
//
LOCUS       DAXWIQ010000110          935 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000110 DAXWIQ010000000
VERSION     DAXWIQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.110"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..360
                     /gene="tilS"
                     /locus_tag="ACGEUI_001781"
     CDS             <1..360
                     /gene="tilS"
                     /locus_tag="ACGEUI_001781"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFF8166582.1"
                     /translation="EEKMLEYDSNLCYRDYYFSFMPKSNEDQGQVSIPLFSLSSIKLR
                     SRQSGDYISFGHFSKKIRRLFIDEKFTIAERQNAIIGEQDEQIIFVLIGNKTYLRKAC
                     KHDIMLAKLYIDKLEKG"
     gene            365..907
                     /gene="hpt"
                     /locus_tag="ACGEUI_001782"
     CDS             365..907
                     /gene="hpt"
                     /locus_tag="ACGEUI_001782"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFF8166583.1"
                     /translation="MLEQDIQKILYSENDIIRKTKKLGEQLTKDYQEKNPLMIGVLKG
                     SVPFMAELMKHIDTHVEIDFMVVSSYHGGTSSSGEVKILKDVDTNIEGRDIIIVEDII
                     DTGRTLKYLRDMFKYRKANTIKIATLFDKPEGRVVKIEADYVCYNIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEVYSK"
BASE COUNT          362 a          109 c          165 g          299 t
ORIGIN      
        1 gaagaaaaaa tgttagaata cgatagtaac ttatgctaca gagattatta tttttcattt
       61 atgccaaaaa gtaacgaaga tcaaggacag gtaagtatac ctttatttag tttatcgtct
      121 attaaattaa gaagtcgcca atctggagat tatatttctt ttggtcactt ttcaaaaaaa
      181 ataagacgtt tatttataga tgaaaaattc acaatagctg aacgtcaaaa tgctattatt
      241 ggagagcaag atgagcagat tatatttgta ttgataggta ataaaactta tttgagaaaa
      301 gcttgtaaac atgatataat gttggctaaa ctgtatattg ataaattaga aaaagggtga
      361 cagcatgctc gagcaagata ttcaaaaaat tctttactct gaaaatgata ttattcggaa
      421 aactaaaaaa ttaggagaac aattaacaaa agattatcaa gaaaaaaatc cgcttatgat
      481 tggtgtatta aaaggatcag ttccttttat ggcagaacta atgaaacaca tcgatactca
      541 tgttgaaatt gattttatgg tagtatcaag ttatcatggc ggtacatcta gcagtggtga
      601 agttaaaatt ttaaaagatg tagataccaa tattgaagga agagatatca taattgttga
      661 agatattatt gacaccggtc gcaccttaaa atatttacga gatatgttta agtaccgtaa
      721 agctaatact atcaaaattg caacactgtt tgataaacct gaaggtcgtg ttgttaaaat
      781 tgaagcagat tatgtttgtt acaatattcc aaatgaattt atcgtaggct ttggtttaga
      841 ttacgcagaa aattatcgca atcttcctta cgtaggtgta ttaaaagaag aagtctattc
      901 aaaatagaaa aaggtcaatg tttactgtat gaaaa
//
LOCUS       DAXWIQ010000111          883 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000111 DAXWIQ010000000
VERSION     DAXWIQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.111"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            22..822
                     /gene="sda3"
                     /locus_tag="ACGEUI_001783"
                     /gene_synonym="mf3"
     CDS             22..822
                     /gene="sda3"
                     /locus_tag="ACGEUI_001783"
                     /gene_synonym="mf3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptodornase Sda3"
                     /protein_id="HFF8166584.1"
                     /translation="MSKSNRRTWQGLVVILIAILTTFTTSTVTAARKIRNFPDTTEIL
                     LGTKATETPGILPFTGSYQLVLGDLDNLQRPTFAHIQLKDQDEPNIKRKGLKFNPPGW
                     HNYKLTDANGKTTWLMDRGHLVGYQFSGLNDEPKNLVTMTKYLNTGFSDKNPLGMLYY
                     ENRLDSWLALHPNFWLDYKVTPVYHKNELVPRQVVLQYVGIDENGDLLQIKLGSEKES
                     VDNFGVTSVTLDNVSPLAELDYQTGMMLDSTQNEEDSNLETEEFEEAA"
BASE COUNT          315 a          153 c          161 g          254 t
ORIGIN      
        1 taataaaagg aggacatgga tatgtctaaa tcaaatcgtc gtacttggca aggtttagtt
       61 gttattttaa tagctattct caccactttt accacaagta ctgttacggc agccagaaaa
      121 attagaaatt tccctgatac cacggaaatt ttgttaggaa cgaaggcgac tgagacacca
      181 ggaatcttac cattcactgg tagctaccaa ttagttttgg gcgatcttga caatctgcaa
      241 aggccaacct tcgcacacat ccagctaaaa gatcaagatg agcctaatat taaacgaaaa
      301 ggacttaaat tcaatcctcc tggctggcat aattacaaat tgactgacgc taatggaaaa
      361 acaacttggt taatggaccg tggccattta gttggttacc aatttagcgg cttaaatgac
      421 gagcctaaaa acctagttac aatgacaaaa tatcttaata ctggctttag tgacaaaaat
      481 cctttaggaa tgctctatta tgaaaataga ttagatagct ggttagctct acaccctaac
      541 ttctggctag actataaagt tactcctgtt tatcataaaa atgagttagt tcctcgccaa
      601 gtagttctac agtatgttgg aattgatgaa aatggagatc tacttcaaat taagttaggt
      661 agtgaaaaag aaagtgtaga caactttgga gtaacatcag ttacattaga taacgtatct
      721 cctttagctg aattggatta ccaaacagga atgatgctag attcaactca aaacgaagaa
      781 gatagtaatt tagaaaccga agagtttgaa gaagcggctt aacataaact tatacttaat
      841 aaaaatatct agattgtgaa cattatccca aatgttagat tat
//
LOCUS       DAXWIQ010000112          835 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000112 DAXWIQ010000000
VERSION     DAXWIQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.112"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            5..802
                     /locus_tag="ACGEUI_001784"
     CDS             5..802
                     /locus_tag="ACGEUI_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8166585.1"
                     /translation="MRFGFLLCLLLYPLAACNKSEQLNHYERIKKTRKLVVAVSPDYA
                     PFEFKALVNGKDTIVGADVQLAQAIADELDVDLELSPMSFDNVLSSLQTGKADLAISG
                     ISHTKERAKVYDFSIPYYQAENAIVMRASDAKVTKNISDLNGKKVAAQKGSIEEGLVK
                     IQLPKANLISLTAMGEAINELKAGQVYAVTLEAPVAAGFLAQHKDLALAPFSLKTSDG
                     DAKAVALPKNSGDLTKAVNKVIAKLDEQERYKSFIAETIALTKNTMK"
BASE COUNT          288 a          136 c          162 g          249 t
ORIGIN      
        1 aaaaataaga tttggttttt tgctttgcct cctactttat cctttagcag cttgtaacaa
       61 gtctgaacaa cttaatcact acgaaagaat taaaaaaaca agaaaattag tcgttgctgt
      121 aagccctgat tatgctcctt ttgaatttaa agctttagtt aatggaaaag atactattgt
      181 tggagcagat gttcaattag ctcaggctat tgctgatgaa ttagacgttg atcttgagct
      241 ttcaccaatg agttttgata atgttttgtc tagtttacag actggtaaag ctgatcttgc
      301 tatttcagga atctcacata ctaaagaaag agctaaagta tatgattttt caattcctta
      361 ctatcaggca gaaaacgcta tagtgatgag agcatctgat gctaaagtga ccaaaaatat
      421 tagtgaccta aacggtaaga aagtagccgc tcaaaaaggt agtatcgagg aaggtttagt
      481 taaaatacaa ttaccaaagg ccaatctgat ttctttgact gctatgggag aagccattaa
      541 tgaactaaaa gcaggacaag tctatgcagt taccttagaa gcacctgtag ctgctggttt
      601 tttagcccaa cataaggatc tggctttagc accctttagc ttaaaaacca gtgatggaga
      661 tgccaaagca gtggcccttc ctaaaaatag tggagactta accaaagccg ttaataaggt
      721 tattgctaag ttagatgagc aagaacggta caagtcattt attgcagaga caatagcact
      781 aacgaaaaat actatgaagt aataaggtta ataaaaactt tattagtatc cgttg
//
LOCUS       DAXWIQ010000113          756 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000113 DAXWIQ010000000
VERSION     DAXWIQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.113"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            187..>756
                     /locus_tag="ACGEUI_001785"
     CDS             187..>756
                     /locus_tag="ACGEUI_001785"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8166586.1"
                     /translation="MTKMYYDENPDSLHDIHEVKVELLNHPFTFLTDSGVFSKKMVDF
                     GSQVLLKTLNFKENERVLDLGCGYGPLGISLAKVQRVDATLVDINNRALDLARKNATN
                     NQVAVTIFQSNIYENISGHFEHIISNPPIRAGKRVVHSIIEKSIDFLVVNGDLTIVIQ
                     KKQGAPSAKAKMATIFGNVEILRKDKGYYV"
BASE COUNT          284 a          103 c          138 g          231 t
ORIGIN      
        1 aaaattaata caattaactc aaaaaagcca cacattccta gtacaattat cctgctatat
       61 tctaacaaat agttaaagtt cggtttttat ttgtaatata gataaaattg ttaaaaaaca
      121 agcgtcttgt aaaccatttc aaagtatgat agaatgaaaa agtaccttaa aaagatagga
      181 aaaatgatga caaagatgta ttatgatgaa aatccagata gcctacacga tattcatgaa
      241 gtaaaagtag agctgcttaa tcatcccttt acttttttga cggattcagg tgtcttttca
      301 aagaaaatgg ttgatttcgg tagccaagta ttattaaaga cactaaattt caaggagaac
      361 gaacgtgtac tggacttagg ttgcggctat ggccctcttg ggattagtct agccaaggtt
      421 caaagggtag acgcaacctt agtggacatc aataatcgtg ctcttgattt agctagaaaa
      481 aatgcaacta ataaccaagt ggcagtaact atttttcaat caaatattta tgaaaatatt
      541 agtggccatt ttgagcatat tattagtaat cctcctatta gagcaggaaa gagagtagtt
      601 catagtatta ttgaaaaaag catagatttt ttagtggtta atggtgactt aacgattgtg
      661 attcaaaaaa aacaaggagc tcctagcgct aaagcaaaaa tggcaactat ttttggtaat
      721 gttgagatat taaggaaaga taaaggttat tatgta
//
LOCUS       DAXWIQ010000114          743 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000114 DAXWIQ010000000
VERSION     DAXWIQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.114"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(<1..637)
                     /locus_tag="ACGEUI_001786"
     CDS             complement(<1..637)
                     /locus_tag="ACGEUI_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8166587.1"
                     /translation="MIKILLVEDDLSLSNSIFDFLDDFADVMQVFDGDEGLYEAESGI
                     YDLILLDLMLPEKNGFQVLKELREKDIKIPVLIMTAKEGLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRTGKFADKNISFGNLVVDLARKEVKVEGKVVELLGKEFDLLVY
                     LLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYISKIRKKLKGTCFVNRLQT"
BASE COUNT          251 a          158 c           77 g          257 t
ORIGIN      
        1 aagtttgaag cctgttaaca aaacaggtac cttttaattt tttccttatt ttagaaatgt
       61 aaacttcaac aaccgaaatc gtagtatcac tatcgaatcc ccataaacga tcaaaaattt
      121 gtgtctttgg taaaataacg ttttgatttt gcaaaagata caccaacaaa tcaaattctt
      181 tgccaagtaa ttcaactact ttcccttcaa ccttcacctc ttttcgagct aagtcaacca
      241 ctaaattgcc aaaactaata tttttatctg caaatttccc tgttcttttt aacagtgctt
      301 gaattcgcat ttttaactct tctaaataga acggctttgt cagataatca tcagcaccta
      361 attcaaatcc gtggccttta tcatctaaac cctctttagc tgtcatgatc aatacaggaa
      421 ttttaatatc tttttctcgc aattctttta agacttgaaa gccatttttc tccggcaaca
      481 taaggtctaa caaaatcaaa tcataaatac cactttctgc ttcgtataaa ccttcatcgc
      541 catcaaaaac ttgcatgacg tctgcaaaat catctaaaaa atcaaaaatg gaatttgata
      601 agctcaagtc atcttctact aataatattt ttatcatgtc tctacctcaa ttgttcttaa
      661 ataaagtaac tatcacttca tttaacttag tctcatttct gataacaatt tctaacgaga
      721 ctatactaca aaaagcttaa ggt
//
LOCUS       DAXWIQ010000115          734 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000115 DAXWIQ010000000
VERSION     DAXWIQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.115"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(<1..671)
                     /locus_tag="ACGEUI_001787"
     CDS             complement(<1..671)
                     /locus_tag="ACGEUI_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFF8166588.1"
                     /translation="MINPSKQNQTIFLWNMLGSLSTAVISVILLMVVTRLLTSADSDI
                     YAFAYSFANMMVVVGLFQVRNYQATDINEKYSFSQYLVARLMTCLLMLAITVIYLTLT
                     KTDSYKSTIVFLVCFYRSTDAFSDLYQGMFQQHERLDIAGKSLAYRNTLIFMVYTAII
                     LYSKNLTLALVAVCIVSLVFIMYYDIGHSKKFQKLMFSELLSNISFQNSLKLLKESFP
                     LFLNG"
     gene            complement(664..>734)
                     /locus_tag="ACGEUI_001788"
     CDS             complement(664..>734)
                     /locus_tag="ACGEUI_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HFF8166589.1"
                     /translation="QNDKVKRLTQEVALMKKEKTND"
BASE COUNT          266 a          118 c          104 g          246 t
ORIGIN      
        1 aaaccgttta gaaaaagtgg aaaactttct tttaataact ttaaactgtt ttgaaatgaa
       61 atattactta ataattcact aaacatcaac ttttgaaatt ttttcgaatg accaatgtca
      121 taatacatta taaaaactaa tgaaactata cagacagcta ctaatgctaa agtaagattt
      181 ttggaatata atattattgc tgtgtacacc ataaaaataa gagtatttct gtaggctagc
      241 gatttacctg caatatccag ccgttcatgt tgttggaaca ttccctgata taaatccgaa
      301 aaagcatctg tcgatctata gaaacagact agaaagacaa ttgtactctt gtagctatct
      361 gtcttagtta acgttaaata aattactgtt atggctaaca ttaataaaca agtcatcagt
      421 cttgccacta agtactgact aaaggaatat ttttcattaa tatctgttgc ttgataatta
      481 cgaacctgaa aaagtccaac aactaccatc atattagcaa aagaataggc aaaggcgtaa
      541 atatctgaat cagccgaagt tagtaaccta gtaaccacca taagtaatat aactgatatt
      601 acagctgttg ataaacttcc taacatattc cataaaaaaa tcgtttggtt ttgttttgaa
      661 ggattaatca ttggttttct cttttttcat aagggccact tcttgggtca gacgttttac
      721 tttatcattt tgaa
//
LOCUS       DAXWIQ010000116          734 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000116 DAXWIQ010000000
VERSION     DAXWIQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.116"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..>734
                     /locus_tag="ACGEUI_001789"
     CDS             <1..>734
                     /locus_tag="ACGEUI_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFF8166590.1"
                     /translation="FFDIDNKKIDLINKRQSPLKEAAINKLLCKAKNINATSSEELAY
                     KDATFIILSLPTNLKFNKLDTSIIEISVSNILKINKKATIVIKSTVPIGFTEYLRNRF
                     HYNDIIFSPEFLREGSTIHDQLYPSRTIVGNESRNSQLFLDILTDISVEKDSPSLLVG
                     SSEAEAIKLFSNAYLAQKIAFFNELDTFAEMQNLDSKKIIEAMGYDQRIGNSHNNPSF
                     GFGGYCLPKDIKQLEYHFKEIPAPIIT"
BASE COUNT          266 a          111 c          114 g          243 t
ORIGIN      
        1 ttttttgata ttgataataa aaaaattgat ttaataaata aaaggcaatc ccctcttaaa
       61 gaagcagcta taaacaaact tttatgtaag gcaaaaaata ttaatgcaac ttcttctgaa
      121 gaattagcat ataaggatgc gactttcata atcttgtctt tgccaaccaa cctaaaattt
      181 aataagcttg atacttccat tatcgaaatt tctgtaagta atattttaaa gataaacaaa
      241 aaggctacaa ttgtaataaa gtcgacagtt ccaattggtt ttacagaata tttaaggaat
      301 cgatttcact acaacgatat cattttttca cctgagttcc ttagggaagg atcaactatt
      361 catgatcaat tgtatccttc gagaactata gttggaaatg aatctagaaa ttctcaatta
      421 ttcttagaca tactaacaga tatatcggtt gaaaaagact cgccatcttt attagttggc
      481 tcttctgaag cagaagcgat aaagttattt tcgaatgcat acttggcaca aaaaattgct
      541 ttttttaatg agttggatac gtttgctgaa atgcaaaatt tggactcaaa aaaaattatt
      601 gaggctatgg gatatgacca gagaatagga aattcgcaca ataatccttc tttcggtttt
      661 ggtgggtact gtcttcctaa ggatattaag caattagagt atcattttaa agaaattcca
      721 gcaccaatta ttac
//
LOCUS       DAXWIQ010000117          703 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000117 DAXWIQ010000000
VERSION     DAXWIQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.117"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..397
                     /locus_tag="ACGEUI_001790"
     CDS             <1..397
                     /locus_tag="ACGEUI_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HFF8166591.1"
                     /translation="VISFVLPTPSLISIGRILLTILLIWLTTLWQIPFILWLSRKINV
                     YFAMIINIISPLIIGTSFSLLNKWYLFPYDWSLKLLEPMTRMRINSIPFGAEFVPDYS
                     QIFISLFLGIAFFILLTNLFAISFKKQVK"
     gene            400..>703
                     /locus_tag="ACGEUI_001791"
     CDS             400..>703
                     /locus_tag="ACGEUI_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8166592.1"
                     /translation="MKSYLCSNFLKIKNTSYLLIHILAALLFPLLLFFYWSQRGGLQS
                     RTVLFSYFQIVGVLLPFVASIVCIQLKNLEESSGKYKYLLGYSQSNYKPFIVELVFL"
BASE COUNT          212 a          105 c          102 g          284 t
ORIGIN      
        1 tgtaattagt tttgtcctac ctactccaag tcttattagt ataggtagga tactattaac
       61 tatcttgtta atttggttga ctacactatg gcaaatcccg tttattttat ggctatcaag
      121 aaaaattaat gtgtattttg ctatgattat taatattata tctccactaa ttattggtac
      181 aagtttttct ctcttaaata aatggtattt gttcccttat gattggtcgt tgaagttgct
      241 tgagccaatg acaagaatga gaataaatag tatacctttt ggagcggagt ttgttccaga
      301 ctactcacag atttttatat cattgttcct aggaattgct tttttcatct tactgaccaa
      361 tctatttgct atctctttca aaaaacaggt gaaatagtca tgaaatcata tttatgttct
      421 aattttttaa aaatcaaaaa tacttcatat ttactaatac atattttagc tgcactatta
      481 tttcccttac ttctattttt ctattggagt caacgtggag gtttacaaag caggactgta
      541 ttattctcct actttcaaat agtaggtgta cttcttcctt ttgttgcaag tattgtgtgc
      601 attcaactaa aaaatttgga agaatcatct ggaaaatata aatatttatt gggttactca
      661 cagtcgaatt ataagccatt tattgtagaa ttagtatttc tat
//
LOCUS       DAXWIQ010000118          676 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000118 DAXWIQ010000000
VERSION     DAXWIQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.119"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            128..283
                     /locus_tag="ACGEUI_001792"
     CDS             128..283
                     /locus_tag="ACGEUI_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166593.1"
                     /translation="MDEKNPLEQFEEFCKNNSFEYVKGWVDGMQSVLNILSMELSKKN
                     GSTSEVD"
     gene            complement(264..>676)
                     /locus_tag="ACGEUI_001793"
     CDS             complement(264..>676)
                     /locus_tag="ACGEUI_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166594.1"
                     /translation="EKFNFWLTIATAVLPAAISAIISFLASRYKNRSDIESLEKKFNH
                     EMTTIEKNHSNDLERLEKQYQHDFEAMEKNHFRELEKMKEAHTLELENIQKQSEAEVN
                     NTVAQGLMTMLGSAMTPALSKYLEENDVFNQPPK"
BASE COUNT          210 a          102 c          115 g          249 t
ORIGIN      
        1 ttactatacc ttgattttag aatatagttt gtaaaatgtc aatactaaag ttgaaaaact
       61 tcgcgattat tttcgcttta cttttaataa aaaaactccc gattgggagt agaaaggaga
      121 gagtagtatg gatgaaaaaa atccactcga acagtttgaa gagttttgca agaataattc
      181 atttgagtat gttaaaggct gggttgatgg catgcaatca gttttaaata ttctctcaat
      241 ggaattatct aaaaaaaatg gatctacttc ggaggttgat tgaatacatc gttttcttct
      301 aaatatttag agagcgctgg tgtcattgct gagcctaaca tggtcattag tccttgagca
      361 acagtattgt ttacttctgc ttctgactgc ttttgaatat tttctaactc taaagtgtga
      421 gcttctttca ttttctcaag ttctcgaaaa tgattttttt ccatagcttc aaaatcatgt
      481 tgatactgtt tttcaagcct ttctaggtca ttactatgat ttttttcgat agtagtcatc
      541 tcatgattga actttttttc taacgattca atatcagatc tgttcttata tcttgaagct
      601 aagaagctaa taatagcaga aattgcagct ggcaaaacag ctgttgcaat agttaaccag
      661 aaattaaatt tttcca
//
LOCUS       DAXWIQ010000119          613 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000119 DAXWIQ010000000
VERSION     DAXWIQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.120"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            55..>613
                     /gene="speA"
                     /locus_tag="ACGEUI_001794"
     CDS             55..>613
                     /gene="speA"
                     /locus_tag="ACGEUI_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeA"
                     /protein_id="HFF8166595.1"
                     /translation="MENNKKVLKKMVFFVLVTFLGLTISQEVFAQQDPDPSQLHRSSL
                     VKNLQNIYFLYEGDPVTHENVKSVDQLLSHDLIYNVSGPNYDKLKTELKNQEMATLFK
                     DKNVDIYSVEYYHLCYLCENAERSACIYGGVTNHEGNHLEIPKKIVVKVSIDGIQSLS
                     FDIETNKKMVTAQELDYKVRKYLTDN"
BASE COUNT          241 a           83 c          102 g          187 t
ORIGIN      
        1 taataacctt tttaaatcta gaggagaacc cagatataaa atggaggaat attaatggaa
       61 aacaataaaa aagtattgaa gaaaatggta ttttttgttt tagtgacatt tcttggacta
      121 acaatctcgc aagaggtatt tgctcaacaa gaccccgatc caagccaact tcacagatct
      181 agtttagtta aaaaccttca aaatatatat tttctttatg agggtgaccc tgttactcac
      241 gagaatgtga aatctgttga tcaactttta tctcacgatt taatatataa tgtttcaggg
      301 ccaaattatg ataaattaaa aactgaactt aagaaccaag agatggcaac tttatttaag
      361 gataaaaacg ttgatattta tagtgtagaa tattaccatc tctgttattt atgtgaaaat
      421 gcagaaagga gtgcatgtat ctacggaggg gtaacaaatc atgaagggaa tcatttagaa
      481 attcctaaaa agatagtcgt taaagtatca atcgatggta tccaaagcct atcatttgat
      541 attgaaacaa ataaaaaaat ggtaactgct caagaattag actataaagt tagaaaatat
      601 cttacagata ata
//
LOCUS       DAXWIQ010000120          604 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000120 DAXWIQ010000000
VERSION     DAXWIQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.121"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            42..362
                     /locus_tag="ACGEUI_001795"
     CDS             42..362
                     /locus_tag="ACGEUI_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8166596.1"
                     /translation="MKKTLLLSTAVLTLAGTVALLTHSVDASTYSYYSTSHFLRQDGR
                     ETLREAKYAAELQIRNLLSQYHINGKEYNNYFRYYYRQAMQARNIDEVNKIIEDLEKN
                     LQAQ"
BASE COUNT          220 a           89 c           95 g          200 t
ORIGIN      
        1 ttataattta tagcacatgt aattaaaaaa ggaaggtttt tatgaaaaaa acactattat
       61 taagtactgc tgttttgacg ctagccggta ctgtcgccct attaactcat tctgtagatg
      121 catcgactta ttcttactac agcactagtc attttttaag acaagatgga agagagactc
      181 ttagagaagc taaatatgcg gcagagttac aaattagaaa cttgttaagt cagtatcata
      241 ttaatggaaa agaatataat aattatttca gatattatta tagacaagct atgcaagcta
      301 gaaatataga cgaagttaat aaaatcattg aagatttaga aaaaaacttg caagcacaat
      361 aaagtaaaga ttaatccaga tatatatagt agcagagtaa ttctcgctgc tttttatttc
      421 aacgctttac gtttatggat acctcgcaca gtaatgacaa gcgctctttt tggtactttt
      481 attgtcgtaa gaaatgaaga aacgattatt ttattagcca tagctaccct ctagccataa
      541 aaatacctct taagtagatt ttgattattt aacctgtcta cttaagaggt atcatatcat
      601 attt
//
LOCUS       DAXWIQ010000121          536 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000121 DAXWIQ010000000
VERSION     DAXWIQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.122"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(19..92)
                     /locus_tag="ACGEUI_001796"
     tRNA            complement(19..92)
                     /locus_tag="ACGEUI_001796"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Ile,seq:cat)
     gene            complement(113..186)
                     /locus_tag="ACGEUI_001797"
     tRNA            complement(113..186)
                     /locus_tag="ACGEUI_001797"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(150..152),aa:Met,seq:cat)
     gene            complement(192..265)
                     /locus_tag="ACGEUI_001798"
     tRNA            complement(192..265)
                     /locus_tag="ACGEUI_001798"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(229..231),aa:Pro,seq:tgg)
     gene            complement(272..345)
                     /locus_tag="ACGEUI_001799"
     tRNA            complement(272..345)
                     /locus_tag="ACGEUI_001799"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(309..311),aa:Arg,seq:acg)
     gene            complement(362..446)
                     /locus_tag="ACGEUI_001800"
     tRNA            complement(362..446)
                     /locus_tag="ACGEUI_001800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(410..412),aa:Leu,seq:taa)
     gene            complement(455..526)
                     /locus_tag="ACGEUI_001801"
     tRNA            complement(455..526)
                     /locus_tag="ACGEUI_001801"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(492..494),aa:Gly,seq:gcc)
BASE COUNT          145 a          155 c          122 g          114 t
ORIGIN      
        1 taatgattat ctatgatatg gaccttgtag gactcgaacc tacgaccgct cggttatgag
       61 ccgagtgctc taaccagttg agctaaaggt cccaagtcat ccataaaaat aatagcggcg
      121 aaggggatcg aacccccgac ctcccgggta tgaaccggac gctctagcca gctgagctac
      181 accgccatta atcgggaaga caggattcga acctgcgaca ccttggtccc aaaccaagta
      241 ctctaccaag ctgagctact tcccgaactg atgcacccta gaggagtcga acctctaacc
      301 gcctgattcg tagtcaggta ctctatccag ttgagctaag ggtgctattt caaaaaatta
      361 atgccgagga ccggaatcga accggtacga tgtaaaccat cgcaggattt taagtcctgt
      421 gcgtctgcca gttccgccac cccggctcct caaaagcgaa cgacgggatt cgaacccgcg
      481 acccccacct tggcaaggtg atgttctacc actgaactac gttcgcaact aatctc
//
LOCUS       DAXWIQ010000122          506 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000122 DAXWIQ010000000
VERSION     DAXWIQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.123"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(<1..>506)
                     /locus_tag="ACGEUI_001802"
     CDS             complement(<1..>506)
                     /locus_tag="ACGEUI_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFF8166597.1"
                     /translation="KYAIELMTTLGEVALGSQTIFNILFMPAFVMNLLILFFRPHITQ
                     MAIALIRGQIKEFNKIQVQLFAYLGVFSLIALVGSGLFGIPFLSILYGTNLTDYWVDF
                     MLIMLGGSIGSFATVIDNILTAMRKQQLLLIPYTGGFLISLLITNLFVMKYHILGAAL
                     SFLITMLV"
BASE COUNT          208 a           91 c           66 g          141 t
ORIGIN      
        1 caaaccaaca ttgttattaa aaagctcaaa gcagctccta aaatatgata tttcataaca
       61 aaaagattgg taattaataa cgaaatcaaa aatcctcctg tatatggaat aagaagaagt
      121 tgctgttttc tcatagcggt taagatatta tcgataactg tcgcaaaact accaatcgag
      181 cctcctagca taatcaacat aaaatctacc caataatccg taaggttagt accatatagt
      241 attgataaaa agggaatacc aaacaaacca cttccaacta acgctatcag tgaaaaaact
      301 cctaaataag caaacaattg aacttgtatc ttattaaact cttttatttg acctctgatt
      361 aaagcaatag ccatctgtgt aatatgaggt ctaaaaaata aaattagcaa attcataaca
      421 aaggctggca taaataaaat attaaaaatt gtttgagagc ccaaagcaac ttcacctaaa
      481 gtcgtcatta gttcaatagc gtattt
//
LOCUS       DAXWIQ010000123          504 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000123 DAXWIQ010000000
VERSION     DAXWIQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.124"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
BASE COUNT          161 a           66 c           93 g          184 t
ORIGIN      
        1 gtaatcattt tttgatttac agttattatc atgtgttatc tggcgactgt cctgtttaca
       61 agtaagttca caagtatggg ataaaatagt ataaactata gtaaatactg aatagtcaaa
      121 gaaaaatatc ggcataggtc agaagagctg atatgtttaa tactttttgc ggatatctac
      181 tgtatttaaa tgtatttgat atccttgtca aggatatacg tctaaacaag ttcaaaacga
      241 aatcatttgt gattatctta tcaggagttg actcttgata agatttttgt atatcagtac
      301 tgtggtacgt ttagatagct tggcagtgaa tttatagagt gtcaaacaac gagtgttttg
      361 tgttacacta accattagaa gagtctattg ttgagcatat caaggtgtta tcttttcgag
      421 ttgaggtatc atatctagtt gtaatgtcaa acatatctag tatatttaac tatcacgcca
      481 aacgcatgca gaaacttttg tttt
//
LOCUS       DAXWIQ010000124          447 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000124 DAXWIQ010000000
VERSION     DAXWIQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.125"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            34..107
                     /locus_tag="ACGEUI_001803"
     tRNA            34..107
                     /locus_tag="ACGEUI_001803"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:68..70,aa:Ile,seq:gat)
     gene            130..201
                     /locus_tag="ACGEUI_001804"
     tRNA            130..201
                     /locus_tag="ACGEUI_001804"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:163..165,aa:Glu,seq:ttc)
     gene            212..301
                     /locus_tag="ACGEUI_001805"
     tRNA            212..301
                     /locus_tag="ACGEUI_001805"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:248..250,aa:Ser,seq:tga)
     gene            311..384
                     /locus_tag="ACGEUI_001806"
     tRNA            311..384
                     /locus_tag="ACGEUI_001806"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:345..347,aa:Met,seq:cat)
BASE COUNT          106 a           97 c          121 g          123 t
ORIGIN      
        1 ccaatagtaa taatttttcc aagctattag cttgggcgcg tagctcaggt ggttagagtg
       61 cacgcctgat aagcgtgagg tcggtggttc gagtccactc gtgcccatta tctataatta
      121 tattattatg gtccgttggt caaggggtta agacaccgcc ttttcacggc ggtaacacgg
      181 gttcgaatcc cgtacggact atattatatt tggaggatta cccaagtccg gctgaaggga
      241 acggtcttga aaaccgtcag gcgtgtaaaa gcgtgcgtgg gttcgaatcc cacatcctcc
      301 ttttaactat cgcgggatgg agcagttagg tagctcgtcg ggctcataac ccgaaggtcg
      361 taggttcaaa tcctgctccc gcaatacata agtatgtagt tgttggctcg gtagctcagt
      421 tggtagagca atggattgaa gctccat
//
LOCUS       DAXWIQ010000125          425 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000125 DAXWIQ010000000
VERSION     DAXWIQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.126"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
BASE COUNT          145 a           51 c           87 g          142 t
ORIGIN      
        1 gtgggttaga tgccagtttg gatttttgat atttgtgttg agtttaaaag ataaaaacac
       61 ttaatgaaaa tctgtttttt cttagaaagg ggaccagtat gattgagacg ctaatgagta
      121 cgttggaaat ggttagaaaa aaatcctagt aggactgcaa tcgaagatga ctttgaggaa
      181 tataaaaaga gaaatctacc aatctttgtc agtgaagatg atagtgtgat ctattataga
      241 ccagggttca tttttaatga attctatccc aatatttcag gctggagaca agaactaaag
      301 actaagctta ctagtatttc tttaatttct ttacatttta ttggtgttgt tgacgaacaa
      361 aaaaaggggg gaatatctag gttctttttc tgatacatct gaattcttaa gtagacatcc
      421 aaaaa
//
LOCUS       DAXWIQ010000126          423 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000126 DAXWIQ010000000
VERSION     DAXWIQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.127"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(<1..422)
                     /locus_tag="ACGEUI_001807"
     CDS             complement(<1..422)
                     /locus_tag="ACGEUI_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFF8166598.1"
                     /translation="MDEIHRILGIDEVYKAPKRLTDILFDKDSREDIFRQFLKYETDV
                     SYDWFMQYFEEEQADRKNKKQDFTPKSVSTLLSKIISGNQYYEVAVGTGGILIQAWQE
                     QRLNDSPFTYRPSKYWYHVEELSDKAVPFLLFNMSIRG"
BASE COUNT          114 a           87 c           71 g          151 t
ORIGIN      
        1 atccctctta tagacatatt aaagagtaga aacggtactg ctttgtccga taactcttct
       61 acgtgatacc agtatttact cggacgataa gtaaatggac tgtcatttaa tcgttgttct
      121 tgccatgctt ggataagtat cccacccgtc ccgactgcta cctcgtagta ttgattacca
      181 cttattattt tagataatag tgtactaacc gatttaggcg taaagtcttg ctttttattt
      241 ttgcggtcag cttgttcttc ctcaaaatat tgcataaacc agtcgtaaga tacatctgtt
      301 tcatatttca aaaactgtct aaatatatcc tcacgactat ctttatcaaa gagtatgtcc
      361 gtaagtcgtt tgggtgcttt ataaacttcg tcgatgccta gtatgcgatg tatctcatcg
      421 atc
//
LOCUS       DAXWIQ010000127          419 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000127 DAXWIQ010000000
VERSION     DAXWIQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.128"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..353
                     /locus_tag="ACGEUI_001808"
     CDS             <1..353
                     /locus_tag="ACGEUI_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFF8166599.1"
                     /translation="GIGMTRNTIDKDGQLSQENRYFIFSFKPDVLTFANCVRGHWQIE
                     SMHWLLDVVYHEDHHQTLDKRAAFNLNLIRKMCLYFLKVMVFPKKDLSYRRKQRYISV
                     HLEDYLVQLFGERG"
BASE COUNT          134 a           63 c           92 g          130 t
ORIGIN      
        1 gtggtattgg gatgactcgt aacacgattg ataaggatgg tcagctgagt caagagaatc
       61 gttattttat ctttagcttt aagccggatg tcctcacatt tgccaattgt gtacgaggtc
      121 attggcagat agagagtatg cactggttat tggacgttgt ttatcatgaa gatcatcatc
      181 agacattgga taaaagagcc gcatttaacc taaatcttat ccgaaaaatg tgcttatatt
      241 ttctcaaagt gatggtattt cctaaaaaag acctcagtta tcgtcgcaaa caacggtata
      301 tttctgtcca tttggaagat tatttagtcc aattatttgg agaaagaggc taagcagatg
      361 attgatttaa aaaggaatct tttcaaaatt gatgtccggg cacaaaagga tgaagagaa
//
LOCUS       DAXWIQ010000128          409 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000128 DAXWIQ010000000
VERSION     DAXWIQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.129"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
BASE COUNT          150 a           59 c           70 g          130 t
ORIGIN      
        1 ttgaaggaaa aaagaggtct tcaccatggt taagcaaatg atcttagtac taaggttaaa
       61 agattatcgt gaaataaagt tttgacacaa gtgtaattaa tagaggagct tgaagtatcc
      121 ggtaaaacta tcttgaagtg aacaaacgag taaacctccc ctgataaata caatatacta
      181 gccatttcag agccattaag agtttccagt gaagtgcttt tgatagagag ctaagaaaaa
      241 aacagtcaag gtttaagaac tgcaaattag catttaaatt attttttgtt aatgacagtt
      301 gtctttatta ccataactat aacttcttat aatatagtct tcttaattca tcagtggtgg
      361 ttcgtctcat atctgacctt aaaatattaa actgatcatc agatgatag
//
LOCUS       DAXWIQ010000129          332 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000129 DAXWIQ010000000
VERSION     DAXWIQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.130"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(54..135)
                     /locus_tag="ACGEUI_001809"
     tRNA            complement(54..135)
                     /locus_tag="ACGEUI_001809"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(99..101),aa:Leu,seq:tag)
     gene            complement(141..213)
                     /locus_tag="ACGEUI_001810"
     tRNA            complement(141..213)
                     /locus_tag="ACGEUI_001810"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(178..180),aa:Lys,seq:ttt)
     gene            complement(245..317)
                     /locus_tag="ACGEUI_001811"
     tRNA            complement(245..317)
                     /locus_tag="ACGEUI_001811"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(282..284),aa:Asp,seq:gtc)
BASE COUNT           87 a           95 c           68 g           82 t
ORIGIN      
        1 tctgattaca aatcagatgc tctaccaact gagctaagcc ggctaatctt ttatgcgggt
       61 taagggactt gaacccccac gccgttaagc gccagatcct aaatctggtg cgtctgccaa
      121 ttccgccaaa cccgctttta tgacccgtac tgggctcgaa ccagtgaccc attgattaaa
      181 agtcaattgc tctaccaact gagctaacga gtctttcctt caaaaaatat gactactctt
      241 tgaacggtcc cgacgggaat cgaacccgcg atcttcgccg tgacagggcg acgtgataac
      301 cgctacacta cgggacctgt attttccaat ac
//
LOCUS       DAXWIQ010000130          327 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000130 DAXWIQ010000000
VERSION     DAXWIQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.131"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(69..>327)
                     /locus_tag="ACGEUI_001812"
     CDS             complement(69..>327)
                     /locus_tag="ACGEUI_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase 2 family protein"
                     /protein_id="HFF8166600.1"
                     /translation="NFESHSRQFIKSSDILSSNDIENLKLRALFLLQEEKQYLHGLSE
                     VEWDGILSNTINNIYRYSSEEHSLYLLPLYLLRVYNYLKYN"
BASE COUNT          118 a           60 c           35 g          114 t
ORIGIN      
        1 taagctaaac atattgaaac atattccacc aatcacactc cttctcaaga ttaataaaaa
       61 ttgaaaactt agttgtactt tagataattg tatactctca ataaataaag tggtaataaa
      121 tataacgaat gttcctcact actatatcta taaatattgt taattgtatt agacaaaatt
      181 ccgtcccatt ctacctctga cagcccatgt agatactgtt tttcttcctg taataaaaac
      241 aacgctctta actttaaatt ctcgatatca ttgctggata ggatatcact ggacttaata
      301 aactgtcttg aatgactttc aaaattt
//
LOCUS       DAXWIQ010000131          312 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000131 DAXWIQ010000000
VERSION     DAXWIQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.132"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            <1..>312
                     /locus_tag="ACGEUI_001813"
     CDS             <1..>312
                     /locus_tag="ACGEUI_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFF8166601.1"
                     /translation="LIVKPTSFLASIGVKKVYNFSELQQAVSQMLNVKFPVYIASGVY
                     ELGELYNLEPRVLVEEFIDGEEYSLESVVRNGIYTPLGITKKIVDEKLFMDEIGHIFP
                     SN"
BASE COUNT          115 a           34 c           63 g          100 t
ORIGIN      
        1 ttaatagtga aaccaacctc ttttttagca agtataggag tgaaaaaagt ttataatttt
       61 tcagaattac aacaagcagt ttctcaaatg cttaatgtaa aatttccggt ttacatagct
      121 tcaggggtat atgaattggg ggaattatat aatttagaac ctagagtttt ggtggaagag
      181 tttattgatg gagaagaata tagcttagag agcgtggtaa gaaatggaat ctatacacct
      241 ctagggataa ctaaaaaaat agtggatgaa aaattattta tggatgaaat aggccatatt
      301 ttcccttcaa at
//
LOCUS       DAXWIQ010000132          304 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000132 DAXWIQ010000000
VERSION     DAXWIQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.133"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
     gene            complement(110..292)
                     /locus_tag="ACGEUI_001814"
     CDS             complement(110..292)
                     /locus_tag="ACGEUI_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HFF8166602.1"
                     /translation="MLTYDEFKQAIDNGYIAGDTVAIVRKDGQIFDYVLPHEKVKNGE
                     VVTKEKVEEVLVELSR"
BASE COUNT           85 a           81 c           35 g          103 t
ORIGIN      
        1 aaataacgaa actttatata attgcttcga atcaactatg taagttttaa acttatgccc
       61 cctatttacc cctttaataa aactacttct cagggggcaa agaaaatact tatctcgaaa
      121 gctccaccag cacctcttcc actttttcct tagtcacaac ttctccattc tttactttct
      181 catgtggcaa cacataatca aaaatctgtc cgtctttacg cacgatcgct actgtatcgc
      241 ctgcgatata tccattgtca atcgcttgct taaactcgtc gtatgttagc atttttaccc
      301 tcct
//
LOCUS       DAXWIQ010000133          265 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS32720
            SAMN39934860-rid21391653.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXWIQ010000133 DAXWIQ010000000
VERSION     DAXWIQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39934860
            Sequence Read Archive: SRR27958661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002131022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002131022.1
            Genome Coverage       :: 336x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 16:20:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 1,814
            CDSs (total)                      :: 1,772
            Genes (coding)                    :: 1,718
            CDSs (with protein)               :: 1,718
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 26 of 54
            Pseudo Genes (internal stop)      :: 20 of 54
            Pseudo Genes (multiple problems)  :: 18 of 54
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39934860-rid21391653.denovo.135"
                     /strain="MGAS32720"
                     /serotype="M1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /geo_loc_name="United Kingdom: Scotland"
                     /lat_lon="55.8617 N 4.2583 W"
                     /collection_date="2018"
                     /collected_by="Scottish Microbiology Reference Laboratory"
                     /note="genotype: emm1"
BASE COUNT          100 a           51 c           34 g           80 t
ORIGIN      
        1 acttttttaa acttaccttt tgctttcaaa aatcctaact tcaactattt gcaaatatga
       61 aaaaaactcc ccctacctgc ggaaatgata tactaataga aggatgactg accattatag
      121 agtgcatgct ttagaacttg gcttaactga agagaaaact actaacttta tgccccaaat
      181 ataaaaaaag aaccttgtcg ctatcaataa acatcgatag caacaagatt tttctagacg
      241 aattatttaa cagcgtcttt taaag
//
